Sodium channel protein
|
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30003510 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Sodium channel protein vs. Ensembl Human
Match: SCN5A (sodium voltage-gated channel alpha subunit 5 [Source:HGNC Symbol;Acc:HGNC:10593]) HSP 1 Score: 867.84 bits (2241), Expect = 0.000e+0 Identity = 525/1181 (44.45%), Postives = 744/1181 (63.00%), Query Frame = 2
Query: 2126 NNINEPEMKKR-ISALSVF-----ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLY----------------------------STYNHIGKKLMKIIRRHRQIKY-----PFPWDSNESNPINIQAAIIKMN------------KNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDS--NNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKI-------DKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
+ EP ++R +SA+SV E E K R + + WECCP + +K+ V +V+DPF +L IT+CIVLNT+FM+++ Y+ + ++ NL F IFT E K+IA P YF + WN+FD +IV+LS++EL S+ NLS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y ++ D+G +PRW+ DF H+F+I+FR+LCGE++E+MWDCM V+G C+ FL M++GNLV+LNLFLALLLSSFS+++L DE ++N LQ AL R+ R + +VK + + Y+ + K+ ++ ++ P + P+ + A+ + + E E S+ + ++ + + N + PD+ + P DC + VR C CC+ K WW +R + + IV+H +FETFI+ MI+ SS L ED +L RK +K +LE+ D FT +F++EM+LKW A G KYF +AWCWLDF IV V+L+S + G K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF +C++ ++ L+ ++V NKS C L T + W ++NFDNV GYL+L Q+ATFKGW DIM AVD QP++ N + YIYFV+FIIFG+FFTLNLFIGVIIDNFN QKKK+GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + ++I+ Q FD+ IM I LN + M +E+ Q E + L +N +FV IF EC++K+ ALR YYF WN+FDF++VI SI V+ + SPTL RVIR+ RIGR+LRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIY+I G++ F+ V + GID FNF+TF +M+ LFQI+TS GW+ +L ++N C + CGS + +L+ +Y++ISFL+++NMYIA+ILENFS AT++ L++DD+D+FYE WE++DP AT F+E LS+F + EPL I +PN+I L +++P+ G I C+D++ TK LGE
Sbjct: 651 DGFEEPGARQRALSAVSVLTSALEELEESRHKCPPCWNRLAQRYLIWECCPLWMSIKQGVKLVVMDPFTDLTITMCIVLNTLFMALEHYNMTSEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSR-----MSNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRD-----SDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTA-PDEDREMNNLQLALARIQRGLRFVKRTTWDFCCGLLRQRPQKPAALAAQGQLPSCIATPYSPPPPETEKVPPTRKETRFEEGEQPGQGTPGDPEPVCVPIAVAESDTDDQEEDEENSLGTEEESSKQTPEDSCSEGS-TADMTNTAELLEQIPDLGQDV----KDPEDCFTEGC----VRRCPCCAVDTTQAPGKVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSQCESLNLTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKINILAKINLLFVAIFTGECIVKLAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPTLPNSNGSRGDCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYSVLSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGE 1810 HSP 2 Score: 333.183 bits (853), Expect = 1.922e-91 Identity = 182/368 (49.46%), Postives = 254/368 (69.02%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENW-----QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
L+A K LP G+ P ++ G+ LEDLDPFY+ ++TF+V+N KTIFRFS+T A ++ SPF+P+R AA++IL HSLF+ ++ TI+ NC+ M ++ ++ VE FTAIYT E ++K LARG ++ FT+LRD WNWLDF VI +AY+ N LG LSALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D +VLTVF LS+FALIGLQ++ G L HKCV+ F + + E D L+W Y ++PEN+ + + C N + TC E Y C++ ENP+ GYTSFD+F + L FRLMTQD WE LYQ +R+ G ++MI+FM+VIF GSFYL+N+IL++VAM+Y
Sbjct: 58 LQASKKLPDLYGN--PPQELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYVSE-NIKLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNGTNGSVEADG-LVWESLDLYLSDPENYLLKNGTSDVLLCGNSSD----AGTCPEGYRCLKA-GENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAY 416 HSP 3 Score: 86.2705 bits (212), Expect = 5.747e-16 Identity = 89/348 (25.57%), Postives = 153/348 (43.97%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF-FKCV----------------------------DSNNVRLHES-----LVQNKSD---CYE----LQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKM 4396
IR +A KI+ H F I+ I+ + + + D T+ +++ FT I+ E ++K A G + +D W WLDF+++++A +S + G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV D N L L N SD C E L+ EN + +FD+ +L+LF++ T W + Q + Y I+F+L I G+F+ +NL + V+ + Q + E + +KR+ AM+ +
Sbjct: 120 IRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTK--------YVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYVS--ENIKLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNGTNGSVEADGLVWESLDLYLSDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPD--HGYTSFDSFAWAFLALFRLMTQDCWERLYQ----------QTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETE----EKEKRFQEAMEML 441 HSP 4 Score: 61.2326 bits (147), Expect = 2.614e-8 Identity = 71/318 (22.33%), Postives = 145/318 (45.60%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSAL-----GKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNG---------DVHM-IYFMMVIFFGSFYLINVILSIV 1474
LR I+ HS F F++ I+++ A+ T E + +FT ++ E ++K +A G Y +AW WLDF+++ ++ + V + L G + +LR+LR LR L+ ++ GM+ VV++L+ AI + + L++ + IF+++G+ ++ G +C+ N + G ++ ++Y T +N + + T + ++ + FDN G L ++ T W ++ + + G +++M IYF++ I FGSF+ +N+ + ++
Sbjct: 1140 LRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFK----KYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK-FGRCI----NQTEG------------DLPLNY-----------TIVNNKSQCESLNLTGELYWTKVKVN----------FDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVI 1415
BLAST of Sodium channel protein vs. Ensembl Human
Match: SCN5A (sodium voltage-gated channel alpha subunit 5 [Source:HGNC Symbol;Acc:HGNC:10593]) HSP 1 Score: 867.84 bits (2241), Expect = 0.000e+0 Identity = 525/1181 (44.45%), Postives = 744/1181 (63.00%), Query Frame = 2
Query: 2126 NNINEPEMKKR-ISALSVF-----ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLY----------------------------STYNHIGKKLMKIIRRHRQIKY-----PFPWDSNESNPINIQAAIIKMN------------KNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDS--NNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKI-------DKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
+ EP ++R +SA+SV E E K R + + WECCP + +K+ V +V+DPF +L IT+CIVLNT+FM+++ Y+ + ++ NL F IFT E K+IA P YF + WN+FD +IV+LS++EL S+ NLS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y ++ D+G +PRW+ DF H+F+I+FR+LCGE++E+MWDCM V+G C+ FL M++GNLV+LNLFLALLLSSFS+++L DE ++N LQ AL R+ R + +VK + + Y+ + K+ ++ ++ P + P+ + A+ + + E E S+ + ++ + + N + PD+ + P DC + VR C CC+ K WW +R + + IV+H +FETFI+ MI+ SS L ED +L RK +K +LE+ D FT +F++EM+LKW A G KYF +AWCWLDF IV V+L+S + G K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF +C++ ++ L+ ++V NKS C L T + W ++NFDNV GYL+L Q+ATFKGW DIM AVD QP++ N + YIYFV+FIIFG+FFTLNLFIGVIIDNFN QKKK+GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + ++I+ Q FD+ IM I LN + M +E+ Q E + L +N +FV IF EC++K+ ALR YYF WN+FDF++VI SI V+ + SPTL RVIR+ RIGR+LRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIY+I G++ F+ V + GID FNF+TF +M+ LFQI+TS GW+ +L ++N C + CGS + +L+ +Y++ISFL+++NMYIA+ILENFS AT++ L++DD+D+FYE WE++DP AT F+E LS+F + EPL I +PN+I L +++P+ G I C+D++ TK LGE
Sbjct: 651 DGFEEPGARQRALSAVSVLTSALEELEESRHKCPPCWNRLAQRYLIWECCPLWMSIKQGVKLVVMDPFTDLTITMCIVLNTLFMALEHYNMTSEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSR-----MSNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRD-----SDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTA-PDEDREMNNLQLALARIQRGLRFVKRTTWDFCCGLLRQRPQKPAALAAQGQLPSCIATPYSPPPPETEKVPPTRKETRFEEGEQPGQGTPGDPEPVCVPIAVAESDTDDQEEDEENSLGTEEESSKQTPEDSCSEGS-TADMTNTAELLEQIPDLGQDV----KDPEDCFTEGC----VRRCPCCAVDTTQAPGKVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSQCESLNLTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKINILAKINLLFVAIFTGECIVKLAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPTLPNSNGSRGDCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYSVLSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGE 1810 HSP 2 Score: 333.183 bits (853), Expect = 1.922e-91 Identity = 182/368 (49.46%), Postives = 254/368 (69.02%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENW-----QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
L+A K LP G+ P ++ G+ LEDLDPFY+ ++TF+V+N KTIFRFS+T A ++ SPF+P+R AA++IL HSLF+ ++ TI+ NC+ M ++ ++ VE FTAIYT E ++K LARG ++ FT+LRD WNWLDF VI +AY+ N LG LSALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D +VLTVF LS+FALIGLQ++ G L HKCV+ F + + E D L+W Y ++PEN+ + + C N + TC E Y C++ ENP+ GYTSFD+F + L FRLMTQD WE LYQ +R+ G ++MI+FM+VIF GSFYL+N+IL++VAM+Y
Sbjct: 58 LQASKKLPDLYGN--PPQELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYVSE-NIKLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNGTNGSVEADG-LVWESLDLYLSDPENYLLKNGTSDVLLCGNSSD----AGTCPEGYRCLKA-GENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAY 416 HSP 3 Score: 86.2705 bits (212), Expect = 5.747e-16 Identity = 89/348 (25.57%), Postives = 153/348 (43.97%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF-FKCV----------------------------DSNNVRLHES-----LVQNKSD---CYE----LQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKM 4396
IR +A KI+ H F I+ I+ + + + D T+ +++ FT I+ E ++K A G + +D W WLDF+++++A +S + G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV D N L L N SD C E L+ EN + +FD+ +L+LF++ T W + Q + Y I+F+L I G+F+ +NL + V+ + Q + E + +KR+ AM+ +
Sbjct: 120 IRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTK--------YVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYVS--ENIKLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNGTNGSVEADGLVWESLDLYLSDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPD--HGYTSFDSFAWAFLALFRLMTQDCWERLYQ----------QTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETE----EKEKRFQEAMEML 441 HSP 4 Score: 61.2326 bits (147), Expect = 2.614e-8 Identity = 71/318 (22.33%), Postives = 145/318 (45.60%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSAL-----GKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNG---------DVHM-IYFMMVIFFGSFYLINVILSIV 1474
LR I+ HS F F++ I+++ A+ T E + +FT ++ E ++K +A G Y +AW WLDF+++ ++ + V + L G + +LR+LR LR L+ ++ GM+ VV++L+ AI + + L++ + IF+++G+ ++ G +C+ N + G ++ ++Y T +N + + T + ++ + FDN G L ++ T W ++ + + G +++M IYF++ I FGSF+ +N+ + ++
Sbjct: 1140 LRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFK----KYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK-FGRCI----NQTEG------------DLPLNY-----------TIVNNKSQCESLNLTGELYWTKVKVN----------FDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVI 1415
BLAST of Sodium channel protein vs. Ensembl Human
Match: SCN5A (sodium voltage-gated channel alpha subunit 5 [Source:HGNC Symbol;Acc:HGNC:10593]) HSP 1 Score: 867.455 bits (2240), Expect = 0.000e+0 Identity = 525/1181 (44.45%), Postives = 744/1181 (63.00%), Query Frame = 2
Query: 2126 NNINEPEMKKR-ISALSVF-----ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLY----------------------------STYNHIGKKLMKIIRRHRQIKY-----PFPWDSNESNPINIQAAIIKMN------------KNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDS--NNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKI-------DKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
+ EP ++R +SA+SV E E K R + + WECCP + +K+ V +V+DPF +L IT+CIVLNT+FM+++ Y+ + ++ NL F IFT E K+IA P YF + WN+FD +IV+LS++EL S+ NLS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y ++ D+G +PRW+ DF H+F+I+FR+LCGE++E+MWDCM V+G C+ FL M++GNLV+LNLFLALLLSSFS+++L DE ++N LQ AL R+ R + +VK + + Y+ + K+ ++ ++ P + P+ + A+ + + E E S+ + ++ + + N + PD+ + P DC + VR C CC+ K WW +R + + IV+H +FETFI+ MI+ SS L ED +L RK +K +LE+ D FT +F++EM+LKW A G KYF +AWCWLDF IV V+L+S + G K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF +C++ ++ L+ ++V NKS C L T + W ++NFDNV GYL+L Q+ATFKGW DIM AVD QP++ N + YIYFV+FIIFG+FFTLNLFIGVIIDNFN QKKK+GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + ++I+ Q FD+ IM I LN + M +E+ Q E + L +N +FV IF EC++K+ ALR YYF WN+FDF++VI SI V+ + SPTL RVIR+ RIGR+LRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIY+I G++ F+ V + GID FNF+TF +M+ LFQI+TS GW+ +L ++N C + CGS + +L+ +Y++ISFL+++NMYIA+ILENFS AT++ L++DD+D+FYE WE++DP AT F+E LS+F + EPL I +PN+I L +++P+ G I C+D++ TK LGE
Sbjct: 651 DGFEEPGARQRALSAVSVLTSALEELEESRHKCPPCWNRLAQRYLIWECCPLWMSIKQGVKLVVMDPFTDLTITMCIVLNTLFMALEHYNMTSEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSR-----MSNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRD-----SDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTA-PDEDREMNNLQLALARIQRGLRFVKRTTWDFCCGLLRQRPQKPAALAAQGQLPSCIATPYSPPPPETEKVPPTRKETRFEEGEQPGQGTPGDPEPVCVPIAVAESDTDDQEEDEENSLGTEEESSKQTPEDSCSEGS-TADMTNTAELLEQIPDLGQDV----KDPEDCFTEGC----VRRCPCCAVDTTQAPGKVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSQCESLNLTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKINILAKINLLFVAIFTGECIVKLAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPTLPNSNGSRGDCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYSVLSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGE 1810 HSP 2 Score: 327.405 bits (838), Expect = 1.174e-89 Identity = 180/369 (48.78%), Postives = 254/369 (68.83%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENW-----QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
L+A K LP G+ P ++ G+ LEDLDPFY+ ++TF+V+N KTIFRFS+T A ++ SPF+P+R AA++IL HSLF+ ++ TI+ NC+ M ++ ++ VE FTAIYT E ++K LARG ++ FT+LRD WNWLDF VI +AY +V+ LG +SALR+ RVLRALKT++ I G+KT+V +L+ ++ KL D +VLTVF LS+FALIGLQ++ G L HKCV+ F + + E D L+W Y ++PEN+ + + C N + TC E Y C++ ENP+ GYTSFD+F + L FRLMTQD WE LYQ +R+ G ++MI+FM+VIF GSFYL+N+IL++VAM+Y
Sbjct: 58 LQASKKLPDLYGN--PPQELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYTTEFVD--LGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNGTNGSVEADG-LVWESLDLYLSDPENYLLKNGTSDVLLCGNSSD----AGTCPEGYRCLKA-GENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAY 416 HSP 3 Score: 88.5817 bits (218), Expect = 1.132e-16 Identity = 90/348 (25.86%), Postives = 153/348 (43.97%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF-FKCV----------------------------DSNNVRLHES-----LVQNKSD---CYE----LQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKM 4396
IR +A KI+ H F I+ I+ + + + D T+ +++ FT I+ E ++K A G + +D W WLDF+++++A + F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV D N L L N SD C E L+ EN + +FD+ +L+LF++ T W + Q + Y I+F+L I G+F+ +NL + V+ + Q + E + +KR+ AM+ +
Sbjct: 120 IRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTK--------YVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYTTEF--VDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNGTNGSVEADGLVWESLDLYLSDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPD--HGYTSFDSFAWAFLALFRLMTQDCWERLYQ----------QTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETE----EKEKRFQEAMEML 441 HSP 4 Score: 61.2326 bits (147), Expect = 2.843e-8 Identity = 71/318 (22.33%), Postives = 145/318 (45.60%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSAL-----GKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNG---------DVHM-IYFMMVIFFGSFYLINVILSIV 1474
LR I+ HS F F++ I+++ A+ T E + +FT ++ E ++K +A G Y +AW WLDF+++ ++ + V + L G + +LR+LR LR L+ ++ GM+ VV++L+ AI + + L++ + IF+++G+ ++ G +C+ N + G ++ ++Y T +N + + T + ++ + FDN G L ++ T W ++ + + G +++M IYF++ I FGSF+ +N+ + ++
Sbjct: 1140 LRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFK----KYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK-FGRCI----NQTEG------------DLPLNY-----------TIVNNKSQCESLNLTGELYWTKVKVN----------FDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVI 1415
BLAST of Sodium channel protein vs. Ensembl Human
Match: SCN2A (sodium voltage-gated channel alpha subunit 2 [Source:HGNC Symbol;Acc:HGNC:10588]) HSP 1 Score: 863.218 bits (2229), Expect = 0.000e+0 Identity = 534/1141 (46.80%), Postives = 749/1141 (65.64%), Query Frame = 2
Query: 2246 WECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVK----LYSTYNHIGKKLMKI-------------IRRHRQIKYP----FPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAEVIF------------------WNQPYDCLPDSIR---CFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
W+CC + +K +V+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS++EL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + ++ ++PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFSS++L +D++ ++N LQ A+ R+ + I +VK K + + K L +I I H I+ + D N + I +++ K +E++ +++P L V + ++E +F ++ D+ E++ QP +S+ CF VR CC E K WW +R + +KIV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ C++ + + S+V N S+C L + + W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY +N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q +E+++ L +N VF+++F ECV+K+I+LR+YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C DK CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F + PL I +PNK++L +++P+ G I C+D++ TK LGE
Sbjct: 607 WDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVSLSLMELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI---SNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDN-EMNNLQIAVGRMQKGIDFVKRKIREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTS-GIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQVYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPDKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFAKLSDFADALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1736 HSP 2 Score: 232.261 bits (591), Expect = 1.978e-60 Identity = 140/303 (46.20%), Postives = 196/303 (64.69%), Query Frame = 2
Query: 647 FVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK--------------VFDNSSIGAETYENDSILLWNIKMDYYNNPENW-----QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
++ TI+ NC+ MT ++ ++NVE FT IYT E +IK LARG + FT+LRD WNWLDF VI AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KC++ F+NS G T N ++ ++N +Y + ++ QN C N + C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 2 LIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSSFEINITSFFNNSLDGNGTTFNRTVSIFNWD-EYIEDKSHFYFLEGQNDALLCGNSSD----AGQCPEGYICVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 296 HSP 3 Score: 78.5666 bits (192), Expect = 1.277e-13 Identity = 41/113 (36.28%), Postives = 67/113 (59.29%), Query Frame = 2
Query: 3644 FTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGG 3970
FT I+ E ++K A G + +D W WLDFT++ A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G
Sbjct: 30 FTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEF--VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMG 140 HSP 4 Score: 68.1662 bits (165), Expect = 1.706e-10 Identity = 76/323 (23.53%), Postives = 142/323 (43.96%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-STCNEEYECIRTDNENPNFGY----TSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR +I+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G VY FT +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H C N+T+ + S N EC N + +FDN G+ L ++ T W + +Y + N ++ +YF++ I FGSF+ +N+ + ++
Sbjct: 1065 LRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQVY-FT---NAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-------------------------------------------CINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVI 1340
BLAST of Sodium channel protein vs. Ensembl Human
Match: SCN2A (sodium voltage-gated channel alpha subunit 2 [Source:HGNC Symbol;Acc:HGNC:10588]) HSP 1 Score: 862.448 bits (2227), Expect = 0.000e+0 Identity = 532/1141 (46.63%), Postives = 746/1141 (65.38%), Query Frame = 2
Query: 2246 WECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYS-----------------TYNHIGKKLMKIIRRHRQIKYP----FPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAEVIF------------------WNQPYDCLPDSIR---CFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
W+CC + +K +V+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS++EL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + ++ ++PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFSS++L +D++ ++N LQ A+ R+ + I +VK K+ + K I H I+ + D N + I +++ K +E++ +++P L V + ++E +F ++ D+ E++ QP +S+ CF VR CC E K WW +R + +KIV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ C++ + + S+V N S+C L + + W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY +N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q +E+++ L +N VF+++F ECV+K+I+LR+YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C DK CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F + PL I +PNK++L +++P+ G I C+D++ TK LGE
Sbjct: 739 WDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVSLSLMELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI---SNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDN-EMNNLQIAVGRMQKGIDFVKRKIREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTS-GIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEVEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQVYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPDKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFAKLSDFADALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1868 HSP 2 Score: 316.235 bits (809), Expect = 3.150e-86 Identity = 183/383 (47.78%), Postives = 251/383 (65.54%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK--------------VFDNSSIGAETYENDSILLWNIKMDYYNNPENW-----QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ LEDLDP+Y +K+TF+V+N K I RFS+T A ++ +PFNP+R AI+IL HSLF+ ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK LARG + FT+LRD WNWLDF VI AY+ +VN LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KC++ F+NS G T N ++ ++N +Y + ++ QN C N + C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 56 LEAGKSLPFIYGDIPPE--MVSVPLEDLDPYYINKKTFIVLNKGKAISRFSATPALYILTPFNPIRKLAIKILVHSLFNMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVN--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSSFEINITSFFNNSLDGNGTTFNRTVSIFNWD-EYIEDKSHFYFLEGQNDALLCGNSSD----AGQCPEGYICVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 428 HSP 3 Score: 84.3445 bits (207), Expect = 2.317e-15 Identity = 51/165 (30.91%), Postives = 89/165 (53.94%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGG 3970
IR A KI+ H F I+ I+ + + +T+ + D + ++ FT I+ E ++K A G + +D W WLDFT++ A ++ F + G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G
Sbjct: 118 IRKLAIKILVHSLFNMLIMCTILTNCVFMTMSNP--------PDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEF--VNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMG 272 HSP 4 Score: 68.1662 bits (165), Expect = 1.959e-10 Identity = 76/323 (23.53%), Postives = 144/323 (44.58%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-STCNEEYECIRTDNENPNFGY----TSFDNFGVSLLCAFRLMTQDYWENLY---------QLIIRTNGDVHM-IYFMMVIFFGSFYLINVILSIV 1474
LR +I+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G VY FT +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H C N+T+ + S N EC N + +FDN G+ L ++ T W ++ +L + +++M +YF++ I FGSF+ +N+ + ++
Sbjct: 1197 LRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQVY-FT---NAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-------------------------------------------CINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVI 1472
BLAST of Sodium channel protein vs. Ensembl Celegans
Match: egl-19 (Voltage-dependent L-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:W6RY76]) HSP 1 Score: 283.878 bits (725), Expect = 9.875e-77 Identity = 216/692 (31.21%), Postives = 366/692 (52.89%), Query Frame = 2
Query: 3509 IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDA-GAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYE-LQKTENVS----WVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPR--PKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXX-----XXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAY--SYGIDATFNFETFGKAMIILFQISTSGGWNSILDGL----------MNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQ--PNKI---RLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGN 5479
+V+H +F +L I+ SS L ED R IL + D FT +F VE+ LK GL + ++A+ LD +V V+L S+ DA K +R LR LRPLRA++R G+K VV +I A+ +I N++LV + +F+IIG+QLF G FF C D + ++ E+ + + YE T+ VS W N+ NFDNV + +SLF ++TF+GW ++ A+D E + P +N+ ++F+ FII AFF +N+F+G +I F + ++ + EL D +R K + KP R P++ + + + ++ F+ VI I++NT+ + + + + D LD N +F +FA E V+KI+AL Y WNVFD ++V+ S I+ + + ++P +I + R+ R+++L+ GIRTLL++ + S AL + LL++L+ FIYA+IG+ F +VA S I NF +F A+++LF+ +T W I+ M+++ ++ +++ +CG+ A Y +S+ ++ ++IN+++AVI++NF T+D + L + F W EYDP A G ++ D+ + KI PLG + P+++ RL + +P+ T+C + L + L + GN
Sbjct: 794 VVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTR-NMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLS--KMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDFNFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTFQNEGEREYENCEL---DKNQR-----KCIEFALKAKPHRRYIPRNRLQYRVWWFVTSRAFEYVIFLIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSF----ID--ITYGKLNPGGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNNNFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGLNESRCGNN-FAYPYFISFFMLCSFLVINLFVAVIMDNFDYLTRDWSI-LGPHHLEEFVRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRLACKRLVSMNMPLNSDGTVCFNATLFALVRTNLKIYTEGN 1466 HSP 2 Score: 139.428 bits (350), Expect = 1.692e-32 Identity = 174/657 (26.48%), Postives = 312/657 (47.49%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKD-ILEFMDLGFTVIFLVEMVLKWFAIGL----TKYFKDAWCWLDFTIVLVALISYF------KVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFK-CVDSNNVRLHES-----LVQNKSDCYELQKTE-------------NVSWV---NSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKK--IGGSLELFMTDSQ-----KRYYHAMKKMSSISP-------QKPVP-------------------RPKSTIAQI-------------FYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRW-YYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGID---ATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSY-ILAVLYLVSYLVISFLVIINMYIAVILENFSQA 5206
IR IV+ K FE IL MI A+ + L + + K+ +LE ++ F V+F +E VLK A+G + Y ++AW LDF IV++ L+S + FD KA+R R LRPLR VS +++V+NA+++A+ + ++ L+ L LI++IIG++LF GK C+D +L + + ++ Q ++ N +W N NFDN L++FQ + +GWTD+M D + Y FV +I G+FF LNL +GV+ F+ +++K G + F Q K Y + + I P +PV RP A++ ++ +Q F +++ ++LNT+V+ E + Q + L N FV++F++E ++K+ +L + Y +N FD +VI SI +V+ V F+ + P + V+R R+ R+ ++ K +R L+ SL+ SL ++ ++ LLL L + I+A++G+ +F +++ NF+TF +A++ +FQI T WN+++ Y G+ ++ +Y + + +++N+++A+ ++N + A
Sbjct: 77 IRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDV---KALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCIDPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIY---------FVTLVILGSFFVLNLVLGVLSGEFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDIEPVNEDEQEDEPVAQTVVGEEADEEGEERVEDVRPSKWAARMKRLEKLNRRCRRACRRLVKSQTFYWLVILLVLLNTLVLTSEHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVL---VYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFGG-KFNFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVM--------YHGIESFGGVGTLGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADA 709 HSP 3 Score: 115.161 bits (287), Expect = 3.691e-25 Identity = 91/334 (27.25%), Postives = 166/334 (49.70%), Query Frame = 2
Query: 560 STKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMT------------KNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALG----KXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNE-------EYEC-IRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
S ++ S NP+R I I+ F F +L I NCIA+ KN+ E +E +F ++T E ++K +A G + + YLR+AWN LDFI++ + + + S + + ALR+ RVLR L+ V+ +P ++ V++++L A+I L +L +F++ I+A+IGL+++ G L C+ + + G Q T C T + K ++ +EC T PN G T+FDNFG+++L F+ ++ + W + +Y + + IYF+ ++ GSF+++N++L +++ +
Sbjct: 64 SERSLLCLSLNNPIRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCI----DPATGQLA----------------------QKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIYFVTLVILGSFFVLNLVLGVLSGEF 371 HSP 4 Score: 94.7449 bits (234), Expect = 6.069e-19 Identity = 134/605 (22.15%), Postives = 271/605 (44.79%), Query Frame = 2
Query: 3635 DLGFTVIFLVEMVLKWFAIGLTKYFKDAW----CWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELF-------------------------------MTDSQKRYYHAMKKMSSISPQKPVPRPKSTIA-------QIFYNIINNQKF--DIVIMCFIMLNTIVMCLESHKQGKELSDA-LDHMNKVFVLIFALECVIKIIALRWYYFKI-----PWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSE--------------------VAYSYGIDAT-------------FNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAV-LYLVSYLVISFLVIINMYIAVILENF 5197
+L F ++F +EM+LK +++G T Y + C++ + +L ++ YF + +R+ R LR + W ++ +V++L+ ++ SI ++LL+ +F +IF+++GMQ+FGGKF N + NFD + L++FQI T + W +M ++ +F IY+++ I G + LN+F+ + +DN E MT ++ R M ++ + S KP+P+ S ++F N++ N + + V+ C I++++ ++ E Q + L++ + F +F +E +K+I + K +N+ D ++V S+ +V+ +A+S +++++RV R+ R LR + A+G++ ++ ++V++ + NI L+ ++ F++AIIGV +F + Y G D T FNF+ G AMI LF +ST GW +L +++N + DK + AV L+ ++++++ ++N+++ ++ F
Sbjct: 509 NLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVLVYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFGGKF---------------------------NFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVMYHGIE----SFGGVGTLGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADADSLTNAEKEEEQQEIEGEDEEFEEGEDEGEEHGMDEPEGDEEMTSARPR---RMSEVPAASTVKPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFC-ILVSSAMLAAEDPLQANSTRNMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRT----DAMS--VVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDG-DPTKPVSKKRVWSNNDFNFDNVGDAMISLFVVSTFEGWPQLLYVAIDSN----EEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTF 1067 HSP 5 Score: 87.4261 bits (215), Expect = 1.165e-16 Identity = 64/211 (30.33%), Postives = 112/211 (53.08%), Query Frame = 2
Query: 2315 ELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNL---SLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGE-YMESMWDCMRVNGIYCIP 2935
E I + IV+NTV ++ Y +S ++ NL F +F FEA +K++A P+ Y S++WNVFD L+VV S +++T K ++ NL + R FR++R+ KL + + L ++ A+ + +++ ++ FI+AV+GMQ FG+ + T ++N NF F + +++FR GE + + M C + C P
Sbjct: 1120 EYVIFLIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSFIDITYGK-LNPGGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKV--ALDDSTSIHRNN------NFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDP 1321 HSP 6 Score: 72.4034 bits (176), Expect = 3.966e-12 Identity = 61/187 (32.62%), Postives = 105/187 (56.15%), Query Frame = 2
Query: 2366 DTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASK-DISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGI 2923
+ Y S + + NLFF+++F+ E +K+ + G Y + ++N FD +V+ SI+E D+ G +S+LR+ RLLRIFK+ K W ++ L + + +L +I +L ++L + + IFA++GMQ+FG +F + PK PR NF F + + VF++L GE W+ + +GI
Sbjct: 493 EHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVLVYFDLMKPLG-VSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFG---GKFNFNPQQPK-----PRANFDTFVQALLTVFQILTGED----WNTVMYHGI 666 HSP 7 Score: 68.1662 bits (165), Expect = 6.383e-11 Identity = 73/349 (20.92%), Postives = 158/349 (45.27%), Query Frame = 2
Query: 512 RTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAM----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYV--NSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGT--LLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTN----GDVH------MIYFMMVIFFGSFYLINVILSIVAMSY 1486
R + ++ T+ + F+ S N R ++ HS F+ VL I+++ + T+N + FT+++T E +K + GL+ ++ ++ R+A+N LD +V+ ++ +V A+ + LR LRVLR L+ + G+K VV +++A+ + + +++T + +FA+IG+Q+++GT L + K+ + G + D + P++ +++N+ +FDN G +++ F + T + W L + I +N G +H ++F+ I +F+++N+ + V +++
Sbjct: 758 RRMSEVPAASTVKPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDG---------------DPTKPVSKKRVWSNNDF------------------------NFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTF 1067 HSP 8 Score: 56.9954 bits (136), Expect = 2.013e-7 Identity = 41/147 (27.89%), Postives = 69/147 (46.94%), Query Frame = 2
Query: 2276 KKIVSFIVLDPFMELFITICIVLNTVFMSI--------DTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYG----PRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTL 2680
K +S + PF E I I N + ++I Y N+ ++ + F+V+FT E +K++A G P Y WN+ DF+IVV+ +V SK +S ++ LR FR+LR +L ++ ++ I R +
Sbjct: 79 KLCISIVEWKPF-EFLILFMICANCIALAIYQPYPAQDSDYKNT-----LLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSK-MSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAM 218
BLAST of Sodium channel protein vs. Ensembl Celegans
Match: egl-19 (Voltage-dependent L-type calcium channel subunit alpha [Source:UniProtKB/TrEMBL;Acc:W6RY76]) HSP 1 Score: 283.878 bits (725), Expect = 1.036e-76 Identity = 216/692 (31.21%), Postives = 366/692 (52.89%), Query Frame = 2
Query: 3509 IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDA-GAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYE-LQKTENVS----WVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPR--PKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXX-----XXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAY--SYGIDATFNFETFGKAMIILFQISTSGGWNSILDGL----------MNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQ--PNKI---RLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGN 5479
+V+H +F +L I+ SS L ED R IL + D FT +F VE+ LK GL + ++A+ LD +V V+L S+ DA K +R LR LRPLRA++R G+K VV +I A+ +I N++LV + +F+IIG+QLF G FF C D + ++ E+ + + YE T+ VS W N+ NFDNV + +SLF ++TF+GW ++ A+D E + P +N+ ++F+ FII AFF +N+F+G +I F + ++ + EL D +R K + KP R P++ + + + ++ F+ VI I++NT+ + + + + D LD N +F +FA E V+KI+AL Y WNVFD ++V+ S I+ + + ++P +I + R+ R+++L+ GIRTLL++ + S AL + LL++L+ FIYA+IG+ F +VA S I NF +F A+++LF+ +T W I+ M+++ ++ +++ +CG+ A Y +S+ ++ ++IN+++AVI++NF T+D + L + F W EYDP A G ++ D+ + KI PLG + P+++ RL + +P+ T+C + L + L + GN
Sbjct: 794 VVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTR-NMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLS--KMTEAECRGEYIHYEDGDPTKPVSKKRVWSNNDFNFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTFQNEGEREYENCEL---DKNQR-----KCIEFALKAKPHRRYIPRNRLQYRVWWFVTSRAFEYVIFLIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSF----ID--ITYGKLNPGGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVALDDSTSIHRNNNFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDPMSDDYHKGGLNESRCGNN-FAYPYFISFFMLCSFLVINLFVAVIMDNFDYLTRDWSI-LGPHHLEEFVRLWSEYDPDAKGRIKHLDVVTLLRKISPPLGFGKLCPHRLACKRLVSMNMPLNSDGTVCFNATLFALVRTNLKIYTEGN 1466 HSP 2 Score: 139.813 bits (351), Expect = 1.283e-32 Identity = 174/657 (26.48%), Postives = 312/657 (47.49%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKD-ILEFMDLGFTVIFLVEMVLKWFAIGL----TKYFKDAWCWLDFTIVLVALISYF------KVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFK-CVDSNNVRLHES-----LVQNKSDCYELQKTE-------------NVSWV---NSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKK--IGGSLELFMTDSQ-----KRYYHAMKKMSSISP-------QKPVP-------------------RPKSTIAQI-------------FYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRW-YYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGID---ATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSY-ILAVLYLVSYLVISFLVIINMYIAVILENFSQA 5206
IR IV+ K FE IL MI A+ + L + + K+ +LE ++ F V+F +E VLK A+G + Y ++AW LDF IV++ L+S + FD KA+R R LRPLR VS +++V+NA+++A+ + ++ L+ L LI++IIG++LF GK C+D +L + + ++ Q ++ N +W N NFDN L++FQ + +GWTD+M D + Y FV +I G+FF LNL +GV+ F+ +++K G + F Q K Y + + I P +PV RP A++ ++ +Q F +++ ++LNT+V+ E + Q + L N FV++F++E ++K+ +L + Y +N FD +VI SI +V+ V F+ + P + V+R R+ R+ ++ K +R L+ SL+ SL ++ ++ LLL L + I+A++G+ +F +++ NF+TF +A++ +FQI T WN+++ Y G+ ++ +Y + + +++N+++A+ ++N + A
Sbjct: 77 IRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDV---KALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCIDPATGQLAQKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIY---------FVTLVILGSFFVLNLVLGVLSGEFSKEREKARARGLFQKFREKQQLEEDLKGYLDWITQAEDIEPVNEDEQEDEPVAQTVVGEEADEEGEERVEDVRPSKWAARMKRLEKLNRRCRRACRRLVKSQTFYWLVILLVLLNTLVLTSEHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVL---VYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFGG-KFNFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVM--------YHGIESFGGVGTLGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADA 709 HSP 3 Score: 115.546 bits (288), Expect = 3.235e-25 Identity = 91/334 (27.25%), Postives = 166/334 (49.70%), Query Frame = 2
Query: 560 STKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMT------------KNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALG----KXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNE-------EYECIR-TDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
S ++ S NP+R I I+ F F +L I NCIA+ KN+ E +E +F ++T E ++K +A G + + YLR+AWN LDFI++ + + + S + + ALR+ RVLR L+ V+ +P ++ V++++L A+I L +L +F++ I+A+IGL+++ G L C+ + + G Q T C T + K ++ +EC T PN G T+FDNFG+++L F+ ++ + W + +Y + + IYF+ ++ GSF+++N++L +++ +
Sbjct: 64 SERSLLCLSLNNPIRKLCISIVEWKPFEFLILFMICANCIALAIYQPYPAQDSDYKNTLLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSKMSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAMIPLLHIALLVLFVILIYAIIGLELFCGKLHSTCI----DPATGQLA----------------------QKDPTPCGTDTEGSAFKCQPSDSLTNMGVRWECSSNTTWPGPNNGITNFDNFGLAMLTVFQCVSLEGWTDVMYWVNDAVGREWPWIYFVTLVILGSFFVLNLVLGVLSGEF 371 HSP 4 Score: 94.7449 bits (234), Expect = 5.857e-19 Identity = 134/605 (22.15%), Postives = 271/605 (44.79%), Query Frame = 2
Query: 3635 DLGFTVIFLVEMVLKWFAIGLTKYFKDAW----CWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELF-------------------------------MTDSQKRYYHAMKKMSSISPQKPVPRPKSTIA-------QIFYNIINNQKF--DIVIMCFIMLNTIVMCLESHKQGKELSDA-LDHMNKVFVLIFALECVIKIIALRWYYFKI-----PWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSE--------------------VAYSYGIDAT-------------FNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAV-LYLVSYLVISFLVIINMYIAVILENF 5197
+L F ++F +EM+LK +++G T Y + C++ + +L ++ YF + +R+ R LR + W ++ +V++L+ ++ SI ++LL+ +F +IF+++GMQ+FGGKF N + NFD + L++FQI T + W +M ++ +F IY+++ I G + LN+F+ + +DN E MT ++ R M ++ + S KP+P+ S ++F N++ N + + V+ C I++++ ++ E Q + L++ + F +F +E +K+I + K +N+ D ++V S+ +V+ +A+S +++++RV R+ R LR + A+G++ ++ ++V++ + NI L+ ++ F++AIIGV +F + Y G D T FNF+ G AMI LF +ST GW +L +++N + DK + AV L+ ++++++ ++N+++ ++ F
Sbjct: 509 NLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVLVYFDLMKPLGVSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFGGKF---------------------------NFNPQQPKPRANFDTFVQALLTVFQILTGEDWNTVMYHGIE----SFGGVGTLGVIVCIYYIVLFICGNYILLNVFLAIAVDNLADADSLTNAEKEEEQQEIEGEDEEFEEGEDEGEEHGMDEPEGDEEMTSARPR---RMSEVPAASTVKPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFC-ILVSSAMLAAEDPLQANSTRNMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRT----DAMS--VVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDG-DPTKPVSKKRVWSNNDFNFDNVGDAMISLFVVSTFEGWPQLLYVAIDSN----EEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTF 1067 HSP 5 Score: 87.4261 bits (215), Expect = 1.104e-16 Identity = 64/211 (30.33%), Postives = 112/211 (53.08%), Query Frame = 2
Query: 2315 ELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNL---SLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGE-YMESMWDCMRVNGIYCIP 2935
E I + IV+NTV ++ Y +S ++ NL F +F FEA +K++A P+ Y S++WNVFD L+VV S +++T K ++ NL + R FR++R+ KL + + L ++ A+ + +++ ++ FI+AV+GMQ FG+ + T ++N NF F + +++FR GE + + M C + C P
Sbjct: 1120 EYVIFLIIVMNTVSLACKHYPSSRGFEDFLDVFNLIFTGVFAFEAVLKIVALNPKNYISDRWNVFDLLVVVGSFIDITYGK-LNPGGTNLISINFFRLFRVMRLVKLLSRGEGIRTLLWTFMKSFQALPYVALLIVLLFFIYAVIGMQFFGKVA--LDDSTSIHRNN------NFHSFPAAILVLFRSATGEAWQDIMLSCSDREDVRCDP 1321 HSP 6 Score: 72.0182 bits (175), Expect = 4.358e-12 Identity = 61/187 (32.62%), Postives = 105/187 (56.15%), Query Frame = 2
Query: 2366 DTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASK-DISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGI 2923
+ Y S + + NLFF+++F+ E +K+ + G Y + ++N FD +V+ SI+E D+ G +S+LR+ RLLRIFK+ K W ++ L + + +L +I +L ++L + + IFA++GMQ+FG +F + PK PR NF F + + VF++L GE W+ + +GI
Sbjct: 493 EHYGQSEWLDHFQTMANLFFVILFSMEMLLKMYSLGFTTYTTSQFNRFDCFVVISSILEFVLVYFDLMKPLG-VSVLRSARLLRIFKVTKYWTSLRNLVSSLLNSLRSIISLLLLLFLFIVIFALLGMQVFG---GKFNFNPQQPK-----PRANFDTFVQALLTVFQILTGED----WNTVMYHGI 666 HSP 7 Score: 68.5514 bits (166), Expect = 5.776e-11 Identity = 73/349 (20.92%), Postives = 158/349 (45.27%), Query Frame = 2
Query: 512 RTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAM----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYV--NSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGT--LLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTN----GDVH------MIYFMMVIFFGSFYLINVILSIVAMSY 1486
R + ++ T+ + F+ S N R ++ HS F+ VL I+++ + T+N + FT+++T E +K + GL+ ++ ++ R+A+N LD +V+ ++ +V A+ + LR LRVLR L+ + G+K VV +++A+ + + +++T + +FA+IG+Q+++GT L + K+ + G + D + P++ +++N+ +FDN G +++ F + T + W L + I +N G +H ++F+ I +F+++N+ + V +++
Sbjct: 758 RRMSEVPAASTVKPIPKASSLFILSHTNSFRVFCNMVVNHSYFTNAVLFCILVSSAMLAAEDPLQANSTRNMILNYFDYFFTSVFTVEITLKVIVFGLVFHKGSFCRNAFNLLDILVVAVSLTSFVLRTDAMSVVKILRVLRVLRPLRAINRAKGLKHVVQCVIVAVKTIGNIMLVTFMLQFMFAIIGVQLFKGTFFLCNDLSKMTEAECRGEYIHYEDG---------------DPTKPVSKKRVWSNNDF------------------------NFDNVGDAMISLFVVSTFEGWPQLLYVAIDSNEEDKGPIHNSRQAVALFFIAFIIVIAFFMMNIFVGFVIVTF 1067 HSP 8 Score: 56.9954 bits (136), Expect = 1.880e-7 Identity = 41/147 (27.89%), Postives = 69/147 (46.94%), Query Frame = 2
Query: 2276 KKIVSFIVLDPFMELFITICIVLNTVFMSI--------DTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYG----PRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTL 2680
K +S + PF E I I N + ++I Y N+ ++ + F+V+FT E +K++A G P Y WN+ DF+IVV+ +V SK +S ++ LR FR+LR +L ++ ++ I R +
Sbjct: 79 KLCISIVEWKPF-EFLILFMICANCIALAIYQPYPAQDSDYKNT-----LLETIEYVFIVVFTIECVLKIVAMGFMFHPSAYLRNAWNILDFIIVVIGLVSTILSK-MSIQGFDVKALRAFRVLRPLRLVSGVPSLQVVLNAILRAM 218
BLAST of Sodium channel protein vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45]) HSP 1 Score: 243.432 bits (620), Expect = 1.885e-64 Identity = 193/667 (28.94%), Postives = 336/667 (50.37%), Query Frame = 2
Query: 3509 IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGL----TKYFKDAWCWLDFTIVLVALISYFKVFDAGA-------FKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQ----KTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKP-VPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYG--IDATFNFETFGKAMIILFQISTSGGWNSILDGLMNN------NTYECKIDK-KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLA-----MLEIPICKGHTI 5416
IV K+FE ++ +I SS++L ED R K +L++MD FT +F EM+LK G+ Y +D W LD +V AL ++ G+ K++R LR LRPL+ + R +K V + ++ ++ ++FN+L+V +F IF++I +QLF GKFF C D N R + + Y+ Q + E W N+DN +N L+LF + T +GW I +++D T + P +++V+F I FF +N+F+ +II F Q G EL D K + + P+ +P K++ + ++ + F+ IM I NT+++ ++ + + L N +F +E ++KI+A YF+ WN FDF+ V+ SI ++ +F + +R+FR R++RL++ IR LL++ V S AL + LL+ ++ FIYAI+G+ +F + + I+ NF++F A+I+LF+ +T GW I+ + + E +K + CGS + + Y S++ +S +++N+++AVI++NF T+D + L D F W +YDP ATG + ++ E + + P+G + RLA + +P+ + T+
Sbjct: 716 IVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNK-VLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKN--RKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQ-----GEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEF----GGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNV-SYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSI-LGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTV 1368 HSP 2 Score: 85.5001 bits (210), Expect = 4.251e-16 Identity = 144/643 (22.40%), Postives = 271/643 (42.15%), Query Frame = 2
Query: 3584 DKFLPT--RKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYF----------KVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCV-----DSNNVRLHESLVQNKS------DCYELQKTENVSWVNSQ---INFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQ-------------------KKKIGGSLELFMTDS------------------QKRYYHAMKKMSSISPQKP---------------------VPRPKSTIAQIFYNIINNQKFDIVIM-----------CFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIAL-RWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATK 5212
++ LP +K L + LE + F IF +E VLK A G Y + W +DF +V+ +++ + D+ + +R +R LRPL+ VS +++V+ +++ A+ + + L+ L +IF+IIG++ + G F + NV NK+ +C T W+ +FDN+ +++FQ T +GWT +M D + Y F+ I+ G+FF LNL +GV+ F + ++++ G LE +T + R A KK+ S Q+ R K I + I IM + LNT + E + Q + +D L + VF+ IF +E ++K+ A+ YF +N FD ++++ S VI V + +++R +R+ RI ++ S +R L+ SL+ S+ ++ ++ LL L + I+A++G+ +F + +F+TF A+I +FQI T WN ++ + + G + ++ ++V L ++ ++N+++A+ ++N + A +
Sbjct: 2 EQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAY---------FIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAA-EVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVS---LRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQ------GGIYSGGWPYSIYFIVLVLFGNY-TLLNVFLAIAVDNLANAQE 624 HSP 3 Score: 80.8777 bits (198), Expect = 9.789e-15 Identity = 60/196 (30.61%), Postives = 103/196 (52.55%), Query Frame = 2
Query: 2294 IVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGE 2881
+V P E FI I NT+ + + Y+N + I+ N +FT E+ +K++A+G R YF + WN FDF+ VV SI + + + +L LR FR R+ +L + T+ +L ++ A+ +C+++ ++ FI+A+VGMQ+FG + TE + N NF F ++ +++FR GE
Sbjct: 1042 LVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVT-EFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIW--LNAATEINRHN------NFQSFFNAVILLFRCATGE 1228 HSP 4 Score: 80.4925 bits (197), Expect = 1.315e-14 Identity = 79/279 (28.32%), Postives = 135/279 (48.39%), Query Frame = 2
Query: 704 ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVI--GLAYMMYVNSALGKX---------XXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSN------NVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVH-MIYFMMVIFFGSFYLINVILSIVAMSY 1486
E E F I+ E ++K +A G +++ +YLR WN +DFIV+ G+ M+ + A LR++RVLR LK V+ IP ++ V+ S+L A+ L +L +F + IFA+IGL+ Y G C +N + + N E P+ C N TS +V +TC +++ PN+G TSFDN G +++ F+ +T + W + + G + YF+ +I GSF+++N++L +++ +
Sbjct: 18 ERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSAC---------------------YNERGEIENVSER---PMP-CTNKTSPMGVYNCDVKGTTCLQKWI-------GPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEF 264 HSP 5 Score: 68.9366 bits (167), Expect = 4.192e-11 Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query: 2336 IVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGE-YMESMWDCMRVNG 2920
+ LNT ++ + Y + F+ IF E +KL A G R YF+ K+N FD +++V S E+ ++ S G +S++R RLLRIFKL W ++ L + ++ +I +L +L + + IFA++GMQ+FG ++ FP + P +F F + + VF++L GE + E M+ + G
Sbjct: 399 VFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFG-ISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRF-------NFPTMH---PYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQG 583 HSP 6 Score: 62.003 bits (149), Expect = 5.421e-9 Identity = 50/181 (27.62%), Postives = 92/181 (50.83%), Query Frame = 2
Query: 569 AFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMT----------KNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMY----VNSALGK----XXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRG 1057
+ F SP NP R I+ F V+T I ++ +++ +N + ++ FT ++ E ++K + +G++++ +Y RD WN LD IV+ A + + GK + +LR LRVLR LKT+ IP +K V ++ ++ + + L++ IFA+I +Q++ G
Sbjct: 699 SMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNG 879
BLAST of Sodium channel protein vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45]) HSP 1 Score: 243.817 bits (621), Expect = 2.565e-64 Identity = 193/667 (28.94%), Postives = 336/667 (50.37%), Query Frame = 2
Query: 3509 IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGL----TKYFKDAWCWLDFTIVLVALISYFKVFDAGA-------FKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQ----KTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKP-VPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEV--AYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNN------NTYECKIDK-KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLA-----MLEIPICKGHTI 5416
IV K+FE ++ +I SS++L ED R K +L++MD FT +F EM+LK G+ Y +D W LD +V AL ++ G+ K++R LR LRPL+ + R +K V + ++ ++ ++FN+L+V +F IF++I +QLF GKFF C D N R + + Y+ Q + E W N+DN +N L+LF + T +GW I +++D T + P +++V+F I FF +N+F+ +II F Q G EL D K + + P+ +P K++ + ++ + F+ IM I NT+++ ++ + + L N +F +E ++KI+A YF+ WN FDF+ V+ SI ++ +F + +R+FR R++RL++ IR LL++ V S AL + LL+ ++ FIYAI+G+ +F + + I+ NF++F A+I+LF+ +T GW I+ + + E +K + CGS + + Y S++ +S +++N+++AVI++NF T+D + L D F W +YDP ATG + ++ E + + P+G + RLA + +P+ + T+
Sbjct: 807 IVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNK-VLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKN--RKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQ-----GEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEF----GGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNV-SYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSI-LGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTV 1459 HSP 2 Score: 100.523 bits (249), Expect = 1.046e-20 Identity = 157/678 (23.16%), Postives = 290/678 (42.77%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPT--RKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYF----------KVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCV-----DSNNVRLHESLVQNKS------DCYELQKTENVSWVNSQ---INFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQ-------------------KKKIGGSLELFMTDS------------------QKRYYHAMKKMSSISPQKP---------------------VPRPKSTIAQIFYNIINNQKFDIVIM-----------CFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIAL-RWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFL---VIINMYIAVILENFSQATK 5212
IR +A I++ FE FIL+ I+ + + L++E + LP +K L + LE + F IF +E VLK A G Y + W +DF +V+ +++ + D+ + +R +R LRPL+ VS +++V+ +++ A+ + + L+ L +IF+IIG++ + G F + NV NK+ +C T W+ +FDN+ +++FQ T +GWT +M D + Y F+ I+ G+FF LNL +GV+ F + ++++ G LE +T + R A KK+ S Q+ R K I + I IM + LNT + E + Q + +D L + VF+ IF +E ++K+ A+ YF +N FD ++++ S VI V + +++R +R+ RI ++ S +R L+ SL+ S+ ++ ++ LL L + I+A++G+ +F + +F+TF A+I +FQI T WN ++ + + + G Y Y + ++V+ ++N+++A+ ++N + A +
Sbjct: 62 IRRNAKAIIEWGPFEYFILLTIIGNCVVLSME-QHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAY---------FIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAA-EVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVS---LRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAI----------ESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQE 715 HSP 3 Score: 98.2117 bits (243), Expect = 5.880e-20 Identity = 97/348 (27.87%), Postives = 165/348 (47.41%), Query Frame = 2
Query: 533 SSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMT------KNSES------ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVI--GLAYMMYVNSALGKX---------XXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSN------NVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVH-MIYFMMVIFFGSFYLINVILSIVAMSY 1486
SS + + F+F+ N +R A I+ F +F+L TII NC+ ++ KN + E E F I+ E ++K +A G +++ +YLR WN +DFIV+ G+ M+ + A LR++RVLR LK V+ IP ++ V+ S+L A+ L +L +F + IFA+IGL+ Y G C +N + + N E P+ C N TS +V +TC +++ PN+G TSFDN G +++ F+ +T + W + + G + YF+ +I GSF+++N++L +++ +
Sbjct: 40 SSNAPVKEKGPSSLFIFAEDNIIRRNAKAIIEWGPFEYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSAC---------------------YNERGEIENVSER---PMP-CTNKTSPMGVYNCDVKGTTCLQKWI-------GPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEF 355 HSP 4 Score: 80.8777 bits (198), Expect = 1.111e-14 Identity = 63/215 (29.30%), Postives = 108/215 (50.23%), Query Frame = 2
Query: 2294 IVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMW-------DCMRVN 2917
+V P E FI I NT+ + + Y+N + I+ N +FT E+ +K++A+G R YF + WN FDF+ VV SI + + + +L LR FR R+ +L + T+ +L ++ A+ +C+++ ++ FI+A+VGMQ+FG + TE + N NF F ++ +++FR GE + + DC R
Sbjct: 1133 LVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVT-EFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIW--LNAATEINRHN------NFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAG 1338 HSP 5 Score: 69.3218 bits (168), Expect = 2.929e-11 Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query: 2336 IVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGE-YMESMWDCMRVNG 2920
+ LNT ++ + Y + F+ IF E +KL A G R YF+ K+N FD +++V S E+ ++ S G +S++R RLLRIFKL W ++ L + ++ +I +L +L + + IFA++GMQ+FG ++ FP + P +F F + + VF++L GE + E M+ + G
Sbjct: 490 VFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFG-ISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRF-------NFPTMH---PYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQG 674 HSP 6 Score: 61.2326 bits (147), Expect = 9.020e-9 Identity = 50/181 (27.62%), Postives = 92/181 (50.83%), Query Frame = 2
Query: 569 AFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMT----------KNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMY----VNSALGK----XXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRG 1057
+ F SP NP R I+ F V+T I ++ +++ +N + ++ FT ++ E ++K + +G++++ +Y RD WN LD IV+ A + + GK + +LR LRVLR LKT+ IP +K V ++ ++ + + L++ IFA+I +Q++ G
Sbjct: 790 SMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNG 970 HSP 7 Score: 53.1434 bits (126), Expect = 2.385e-6 Identity = 48/191 (25.13%), Postives = 82/191 (42.93%), Query Frame = 2
Query: 2135 NEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTY---DNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRL----YFSEKWNVFDFLIVVLSIVEL------TASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTII 2668
N P +K S+L +F +N +R R K W PF E FI + I+ N V +S++ + ++ ++ + + +FM IF E +K+IA+G L Y WN+ DF++VV +V + T + + +L LR R+LR KL ++ ++ I
Sbjct: 42 NAPVKEKGPSSLFIFAEDNIIR-------RNAKAIIEW------------------GPF-EYFILLTIIGNCVVLSMEQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSI 206
BLAST of Sodium channel protein vs. Ensembl Celegans
Match: unc-2 (High voltage activated calcium channel alpha-1 subunit [Source:UniProtKB/TrEMBL;Acc:Q86G45]) HSP 1 Score: 243.432 bits (620), Expect = 2.583e-64 Identity = 193/667 (28.94%), Postives = 336/667 (50.37%), Query Frame = 2
Query: 3509 IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGL----TKYFKDAWCWLDFTIVLVALISYFKVFDAGA-------FKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQ----KTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKP-VPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEV--AYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNN------NTYECKIDK-KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLA-----MLEIPICKGHTI 5416
IV K+FE ++ +I SS++L ED R K +L++MD FT +F EM+LK G+ Y +D W LD +V AL ++ G+ K++R LR LRPL+ + R +K V + ++ ++ ++FN+L+V +F IF++I +QLF GKFF C D N R + + Y+ Q + E W N+DN +N L+LF + T +GW I +++D T + P +++V+F I FF +N+F+ +II F Q G EL D K + + P+ +P K++ + ++ + F+ IM I NT+++ ++ + + L N +F +E ++KI+A YF+ WN FDF+ V+ SI ++ +F + +R+FR R++RL++ IR LL++ V S AL + LL+ ++ FIYAI+G+ +F + + I+ NF++F A+I+LF+ +T GW I+ + + E +K + CGS + + Y S++ +S +++N+++AVI++NF T+D + L D F W +YDP ATG + ++ E + + P+G + RLA + +P+ + T+
Sbjct: 716 IVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNK-VLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNGKFFFCTDKN--RKFANTCHGQFFVYDNQNDPPRVEQREWRLRPFNYDNTINAMLTLFVVTTGEGWPGIRQNSMDTTFEDQGPSPFFRVEVALFYVMFFIVFPFFFVNIFVALIIITFQEQ-----GEAELSEGDLDKNQKQCIDFALNARPRSLFMPEDKNSTKYRIWRLVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVTEF----GGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIWLNAATEINRHNNFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAGSAEINFEKGQTCGSNV-SYAYFTSFVFLSSFLMLNLFVAVIMDNFDYLTRDSSI-LGPHHLDEFIRVWADYDPAATGRIHYSEMYEMLRIMAPPVGFGKKCPYRLAYKHLIRMNMPVAEDGTV 1368 HSP 2 Score: 85.5001 bits (210), Expect = 4.337e-16 Identity = 145/646 (22.45%), Postives = 270/646 (41.80%), Query Frame = 2
Query: 3584 DKFLPT--RKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYF----------KVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCV-----DSNNVRLHESLVQNKS------DCYELQKTENVSWVNSQ---INFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQ-------------------KKKIGGSLELFMTDS------------------QKRYYHAMKKMSSISPQKP---------------------VPRPKSTIAQIFYNIINNQKFDIVIM-----------CFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIAL-RWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFL---VIINMYIAVILENFSQATK 5212
++ LP +K L + LE + F IF +E VLK A G Y + W +DF +V+ +++ + D+ + +R +R LRPL+ VS +++V+ +++ A+ + + L+ L +IF+IIG++ + G F + NV NK+ +C T W+ +FDN+ +++FQ T +GWT +M D + Y F+ I+ G+FF LNL +GV+ F + ++++ G LE +T + R A KK+ S Q+ R K I + I IM + LNT + E + Q + +D L + VF+ IF +E ++K+ A+ YF +N FD ++++ S VI V + +++R +R+ RI ++ S +R L+ SL+ S+ ++ ++ LL L + I+A++G+ +F + +F+TF A+I +FQI T WN ++ + + + G Y Y + ++V+ ++N+++A+ ++N + A +
Sbjct: 2 EQHLPKNDKKALSEWLERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSACYNERGEIENVSERPMPCTNKTSPMGVYNCDVKGTTCLQKWIGPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAY---------FIPLIVLGSFFMLNLVLGVLSGEFAKERERVENRREFLKLRRQQQIERELNGYLEWILTAEEVILKEDRTTEEEKAAIMEARRRAANKKLKQASKQQSTETEEDFEEDEDEMEEEYVDEAERKKRGCCHSVGKFIKQLRIQIRIMVKTQIFYWSVITLVFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAA-EVIWAEVYGGSFGISVMRALRLLRIFKLTSYWVS---LRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRFNFPTMHPYTHFDTFPVALITVFQILTGEDWNEVMYLAI----------ESQGGIYSGGWPYSIYFIVLVLFGNYTLLNVFLAIAVDNLANAQE 624 HSP 3 Score: 80.8777 bits (198), Expect = 1.147e-14 Identity = 63/215 (29.30%), Postives = 108/215 (50.23%), Query Frame = 2
Query: 2294 IVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMW-------DCMRVN 2917
+V P E FI I NT+ + + Y+N + I+ N +FT E+ +K++A+G R YF + WN FDF+ VV SI + + + +L LR FR R+ +L + T+ +L ++ A+ +C+++ ++ FI+A+VGMQ+FG + TE + N NF F ++ +++FR GE + + DC R
Sbjct: 1042 LVTSPPFEYFIMTMICCNTLILMMKYYNNPLFYEEILRLFNTALTAVFTVESILKILAFGVRNYFRDGWNRFDFVTVVGSITDALVT-EFGGHFVSLGFLRLFRAARLIRLLQQGYTIRILLWTFVQSFKALPYVCLLIGMLFFIYAIVGMQVFGNIW--LNAATEINRHN------NFQSFFNAVILLFRCATGEGWQDIMMAAVQGKDCARAG 1247 HSP 4 Score: 80.4925 bits (197), Expect = 1.313e-14 Identity = 79/279 (28.32%), Postives = 135/279 (48.39%), Query Frame = 2
Query: 704 ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVI--GLAYMMYVNSALGKX---------XXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSN------NVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVH-MIYFMMVIFFGSFYLINVILSIVAMSY 1486
E E F I+ E ++K +A G +++ +YLR WN +DFIV+ G+ M+ + A LR++RVLR LK V+ IP ++ V+ S+L A+ L +L +F + IFA+IGL+ Y G C +N + + N E P+ C N TS +V +TC +++ PN+G TSFDN G +++ F+ +T + W + + G + YF+ +I GSF+++N++L +++ +
Sbjct: 18 ERTEPYFMGIFCLECVLKVIAFGFALHKGSYLRSGWNIMDFIVVVSGVVTMLPFSPATQTANQPVDSVDLRTLRAVRVLRPLKLVSGIPSLQVVLKSILCAMAPLLQIGLLVLFAIIIFAIIGLEFYSGAFHSAC---------------------YNERGEIENVSER---PMP-CTNKTSPMGVYNCDVKGTTCLQKWI-------GPNYGITSFDNIGFAMITVFQCITMEGWTTVMYYTNDSLGSTYNWAYFIPLIVLGSFFMLNLVLGVLSGEF 264 HSP 5 Score: 69.3218 bits (168), Expect = 3.325e-11 Identity = 60/196 (30.61%), Postives = 102/196 (52.04%), Query Frame = 2
Query: 2336 IVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGE-YMESMWDCMRVNG 2920
+ LNT ++ + Y + F+ IF E +KL A G R YF+ K+N FD +++V S E+ ++ S G +S++R RLLRIFKL W ++ L + ++ +I +L +L + + IFA++GMQ+FG ++ FP + P +F F + + VF++L GE + E M+ + G
Sbjct: 399 VFLNTCCVASEHYGQPQWFTDFLKYAEFVFLGIFVVEMLLKLFAMGSRTYFASKFNRFDCVVIVGSAAEVIWAEVYGGSFG-ISVMRALRLLRIFKLTSYWVSLRNLVRSLMNSMRSIISLLFLLFLFILIFALLGMQLFGGRF-------NFPTMH---PYTHFDTFPVALITVFQILTGEDWNEVMYLAIESQG 583 HSP 6 Score: 61.2326 bits (147), Expect = 9.345e-9 Identity = 50/181 (27.62%), Postives = 92/181 (50.83%), Query Frame = 2
Query: 569 AFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMT----------KNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMY----VNSALGK----XXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRG 1057
+ F SP NP R I+ F V+T I ++ +++ +N + ++ FT ++ E ++K + +G++++ +Y RD WN LD IV+ A + + GK + +LR LRVLR LKT+ IP +K V ++ ++ + + L++ IFA+I +Q++ G
Sbjct: 699 SMFFLSPTNPFRVLIHSIVCTKYFEMMVMTVICLSSVSLAAEDPVDEENPRNKVLQYMDYCFTGVFACEMLLKLIDQGILLHPGSYCRDFWNILDGIVVTCALFAFGFAGTEGSAGKNLNTIKSLRVLRVLRPLKTIKRIPKLKAVFDCVVNSLKNVFNILIVYFLFQFIFAVIAVQLFNG 879
BLAST of Sodium channel protein vs. Ensembl Fly
Match: para (gene:FBgn0285944 transcript:FBtr0303681) HSP 1 Score: 724.161 bits (1868), Expect = 0.000e+0 Identity = 393/762 (51.57%), Postives = 534/762 (70.08%), Query Frame = 2
Query: 3374 EVIFWNQPYDCLPDSI-RCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVF----DAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKK----KCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPM---MDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKK 5623
E I P DC PDS + F + + +S + W +R F+++++K+FET ++ MI+ SSL L LED LP R L+DIL +MD FTVIF +EM++KW A+G YF +AWCWLDF IV+++LI+ V+ D AF+++RTLRALRPLRAVSRW+GMK+VVNAL+QAIPSIFNVLLVCL+FWLIF+I+G+QLF GK+FKC D N +L ++ N++ C ++EN +WVNS +NFD+V N YL LFQ+ATFKGW IM DA+D EV+ QP N + Y+YFV FIIFG+FFTLNLFIGVIIDNFN QKKK GGSLE+FMT+ QK+YY+AMKKM S P K +PRP+ I + I+ ++KFDI+IM FI LN M L+ + + LD++N +FV+IF+ EC++KI ALR++YF PWN+FD ++VI SI V+ + VSPTL+RV+RV ++GRVLRLVK A+GIRTLLF+L +SLPALFNI LLL L+MFI+AI G+S F V GI+ +NF+TFG++MI+LFQ+STS GW+ +LD ++N + + K CGS + + +L+SYLVISFL++INMYIAVILEN+SQAT+DVQ GLT DDYD++YE W+++DP T ++ LSEF+ + PL I +PNK ++ ++IPIC+G + CVD++D LTK+F GNP+ ++ + T +S+T QRE + AR I R+ AR +
Sbjct: 1230 EDILDEYPADCCPDSYYKKFPILAGD--DDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNAC----ESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFAR--KGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGE 1983 HSP 2 Score: 333.954 bits (855), Expect = 6.356e-92 Identity = 187/372 (50.27%), Postives = 253/372 (68.01%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSES--ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFD-NSSIGAETYENDSILLWNIKMDYYN-NPENW---QNPITF--CHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
D LE G +P L FP + A LED+DP+Y++ TFVV++ K IFRFS++KA ++ PFNP+R AI IL H LFS F++TTI++NCI M + E+ E IFT IYT E +K +ARG I+ FTYLRDAWNWLDF+VI LAY+ + LG L+ALR+ RVLRALKTV +PG+KT+V +++ ++ LRD ++LT+F LS+FAL+GLQ+Y G L KC+K F + S G T EN W DY+N N NW I+F C N + C+++Y C++ NPN+GYTSFD+FG + L AFRLMTQD+WE+LYQL++R G HM++F+++IF GSFYL+N+IL+IVAMSY
Sbjct: 76 DPTLEQGVPIPVRLQGSFPPE-LASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVT-MGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDEN-----W----DYHNRNSSNWYSEDEGISFPLCGNISG----AGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSY 432 HSP 3 Score: 260.381 bits (664), Expect = 2.487e-69 Identity = 168/309 (54.37%), Postives = 218/309 (70.55%), Query Frame = 2
Query: 2237 FCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKII 3163
FC W+CC + ++ VS IV DPF+ELFIT+CIV+NT+FM++D +D + M ++ N FF F EA MKL+A P+ YF E WN+FDF+IV LS++EL LS+LR+FRLLR+FKLAKSW T+NLL +I+GRT+GA+GNL VL II+FIFAV+GMQ+FG+ Y K++ FP +G +PRWNF DF HSFMIVFRVLCGE++ESMWDCM V + CIPFFLAT+++GNLV+LNLFLALLLS+F S SL ++ D NK+ EA R+ R WVK + + I KL I
Sbjct: 795 FCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGL-----EGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDR--FP--DGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADN-DTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQI 1093 HSP 4 Score: 78.5666 bits (192), Expect = 7.047e-14 Identity = 53/184 (28.80%), Postives = 96/184 (52.17%), Query Frame = 2
Query: 3470 TKAWWW------IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV 3988
+KA W IR A I+ H F FI+ I+ + + + +PT +E ++ FT I+ E +K A G Y +DAW WLDF ++ +A ++ D G A+RT R LR L+ V+ G+K +V A+I+++ ++ +V+++ + +F+++G+Q++ G KC+
Sbjct: 130 SKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILM-----IMPTTP----TVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTM--GIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCI 302 HSP 5 Score: 73.559 bits (179), Expect = 2.295e-12 Identity = 77/273 (28.21%), Postives = 137/273 (50.18%), Query Frame = 2
Query: 2126 NNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLL---RIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIP 2935
+N NE + KK +L +F E++ + + + +K P + + IV IV D ++ I + I LN M++D YD S + +++ +N F+VIF+ E +K+ A YF E WN+FD ++V+LSI+ L S I + +LLR R+ R+ +L K + + L + +L A+ N+C++L +++FIFA+ GM F E+ +G +NF F S +++F++ + + D + +N C P
Sbjct: 1538 DNFNE-QKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRW-RPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYH-YFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEK----------SGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAI-INEEACDP 1796 HSP 6 Score: 70.8626 bits (172), Expect = 1.524e-11 Identity = 50/171 (29.24%), Postives = 88/171 (51.46%), Query Frame = 2
Query: 2198 RKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYG----PRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNL 2698
+ +DI+ RF+ + W P ++ ++++ +I++ P LFI I++N + M + T + + F I+TFE+ +K++A G P Y + WN DF+++ L+ V T D+ GNL+ LRTFR+LR K + L TI+G + ++ NL
Sbjct: 120 KGKDIF--RFSASKAMWMLDP-FNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPT-------VESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYV--TMGIDL----GNLAALRTFRVLRALKTVA---IVPGLKTIVGAVIESVKNL 271 HSP 7 Score: 67.3958 bits (163), Expect = 1.465e-10 Identity = 51/213 (23.94%), Postives = 100/213 (46.95%), Query Frame = 2
Query: 3359 DINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFK--IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD 3991
D +E F ++ + + I F V +CC W W++ + IV F E FI + IV +++ + ++ + K ++ +L+ + FT F +E +K A+ YF++ W DF IV ++L+ G +R+ R LR + W + ++++ + + + ++ N+ V + IF+++GMQLFG + D
Sbjct: 772 DDDEDGPTFKDKALEVILKGIDVFCV--WDCC--------WVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDM--NKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQG-LSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKD 971 HSP 8 Score: 64.3142 bits (155), Expect = 1.444e-9 Identity = 53/197 (26.90%), Postives = 94/197 (47.72%), Query Frame = 2
Query: 4490 VIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALR-WYYFKIPWNVFDFIIVIFSI---------GFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSY------GIDATFNFETFGKAMIILFQISTSGGW 5032
+ +C I++NT+ M ++ H KE+ L N F FA+E +K++A+ YYF+ WN+FDFIIV S+ G V+ F + L KS + L+ + ++ AL N+ +L +I+FI+A++G+ +F + + + G +NF F + +I+F++ G W
Sbjct: 824 ITLC-IVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFK-----------------LAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRV-LCGEW 1001 HSP 9 Score: 61.6178 bits (148), Expect = 1.016e-8 Identity = 85/330 (25.76%), Postives = 146/330 (44.24%), Query Frame = 2
Query: 584 SPF----NPLRAAAIRILTHSLFSFFVLTTIIINCIAMT-------KNSESENV----ETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIV-----IGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTC-NEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYW-----------ENLYQLIIRTNGDVHM-IYFMMVIFFGSFYLINVILSIV 1474
SPF LR +++ + F V+T I+++ +A+ + +++ + IFT I+ E +IK LA G VY FT +AW WLDF++ I LA + + ++R+LR LR L+ V+ GMK VV++L+ AI + + L++ + IFA++G+Q++ G KC + N H N ++ C +E Y + + +FD+ G + LC F++ T W E Q I TN ++M +YF+ I FGSF+ +N+ + ++
Sbjct: 1258 SPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVY-FT---NAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYF-KCEDM---------------------------------NGTKLSHEIIPN---RNACESENYTWVNS--------AMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETN--IYMYLYFVFFIIFGSFFTLNLFIGVI 1536 HSP 10 Score: 58.9214 bits (141), Expect = 5.558e-8 Identity = 45/153 (29.41%), Postives = 83/153 (54.25%), Query Frame = 2
Query: 2390 MIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTA----SKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCD 2836
++Y M+++ F VIF E +K +A G ++YF+ W DF+IV+LS++ L A + D+ A + +RT R LR + W+ M ++ + + + +I N+ +V I IFA++G+Q+F +Y + ++ + IP N C+
Sbjct: 1311 ILYYMDRI---FTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRS----MRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACE 1456
BLAST of Sodium channel protein vs. Ensembl Fly
Match: para (gene:FBgn0285944 transcript:FBtr0303702) HSP 1 Score: 724.161 bits (1868), Expect = 0.000e+0 Identity = 393/762 (51.57%), Postives = 534/762 (70.08%), Query Frame = 2
Query: 3374 EVIFWNQPYDCLPDSI-RCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVF----DAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKK----KCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPM---MDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKK 5623
E I P DC PDS + F + + +S + W +R F+++++K+FET ++ MI+ SSL L LED LP R L+DIL +MD FTVIF +EM++KW A+G YF +AWCWLDF IV+++LI+ V+ D AF+++RTLRALRPLRAVSRW+GMK+VVNAL+QAIPSIFNVLLVCL+FWLIF+I+G+QLF GK+FKC D N +L ++ N++ C ++EN +WVNS +NFD+V N YL LFQ+ATFKGW IM DA+D EV+ QP N + Y+YFV FIIFG+FFTLNLFIGVIIDNFN QKKK GGSLE+FMT+ QK+YY+AMKKM S P K +PRP+ I + I+ ++KFDI+IM FI LN M L+ + + LD++N +FV+IF+ EC++KI ALR++YF PWN+FD ++VI SI V+ + VSPTL+RV+RV ++GRVLRLVK A+GIRTLLF+L +SLPALFNI LLL L+MFI+AI G+S F V GI+ +NF+TFG++MI+LFQ+STS GW+ +LD ++N + + K CGS + + +L+SYLVISFL++INMYIAVILEN+SQAT+DVQ GLT DDYD++YE W+++DP T ++ LSEF+ + PL I +PNK ++ ++IPIC+G + CVD++D LTK+F GNP+ ++ + T +S+T QRE + AR I R+ AR +
Sbjct: 1233 EDILDEYPADCCPDSYYKKFPILAGD--DDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNAC----ESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFAR--KGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGE 1986 HSP 2 Score: 333.954 bits (855), Expect = 5.903e-92 Identity = 187/372 (50.27%), Postives = 253/372 (68.01%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSES--ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFD-NSSIGAETYENDSILLWNIKMDYYN-NPENW---QNPITF--CHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
D LE G +P L FP + A LED+DP+Y++ TFVV++ K IFRFS++KA ++ PFNP+R AI IL H LFS F++TTI++NCI M + E+ E IFT IYT E +K +ARG I+ FTYLRDAWNWLDF+VI LAY+ + LG L+ALR+ RVLRALKTV +PG+KT+V +++ ++ LRD ++LT+F LS+FAL+GLQ+Y G L KC+K F + S G T EN W DY+N N NW I+F C N + C+++Y C++ NPN+GYTSFD+FG + L AFRLMTQD+WE+LYQL++R G HM++F+++IF GSFYL+N+IL+IVAMSY
Sbjct: 65 DPTLEQGVPIPVRLQGSFPPE-LASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVT-MGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDEN-----W----DYHNRNSSNWYSEDEGISFPLCGNISG----AGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSY 421 HSP 3 Score: 260.766 bits (665), Expect = 2.269e-69 Identity = 168/309 (54.37%), Postives = 218/309 (70.55%), Query Frame = 2
Query: 2237 FCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKII 3163
FC W+CC + ++ VS IV DPF+ELFIT+CIV+NT+FM++D +D + M ++ N FF F EA MKL+A P+ YF E WN+FDF+IV LS++EL LS+LR+FRLLR+FKLAKSW T+NLL +I+GRT+GA+GNL VL II+FIFAV+GMQ+FG+ Y K++ FP +G +PRWNF DF HSFMIVFRVLCGE++ESMWDCM V + CIPFFLAT+++GNLV+LNLFLALLLS+F S SL ++ D NK+ EA R+ R WVK + + I KL I
Sbjct: 763 FCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGL-----EGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDR--FP--DGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADN-DTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQI 1061 HSP 4 Score: 78.5666 bits (192), Expect = 6.109e-14 Identity = 53/184 (28.80%), Postives = 96/184 (52.17%), Query Frame = 2
Query: 3470 TKAWWW------IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV 3988
+KA W IR A I+ H F FI+ I+ + + + +PT +E ++ FT I+ E +K A G Y +DAW WLDF ++ +A ++ D G A+RT R LR L+ V+ G+K +V A+I+++ ++ +V+++ + +F+++G+Q++ G KC+
Sbjct: 119 SKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILM-----IMPTTP----TVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTM--GIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCI 291 HSP 5 Score: 73.559 bits (179), Expect = 2.375e-12 Identity = 77/273 (28.21%), Postives = 137/273 (50.18%), Query Frame = 2
Query: 2126 NNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLL---RIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIP 2935
+N NE + KK +L +F E++ + + + +K P + + IV IV D ++ I + I LN M++D YD S + +++ +N F+VIF+ E +K+ A YF E WN+FD ++V+LSI+ L S I + +LLR R+ R+ +L K + + L + +L A+ N+C++L +++FIFA+ GM F E+ +G +NF F S +++F++ + + D + +N C P
Sbjct: 1541 DNFNE-QKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRW-RPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYH-YFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEK----------SGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAI-INEEACDP 1799 HSP 6 Score: 70.8626 bits (172), Expect = 1.550e-11 Identity = 50/171 (29.24%), Postives = 88/171 (51.46%), Query Frame = 2
Query: 2198 RKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYG----PRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNL 2698
+ +DI+ RF+ + W P ++ ++++ +I++ P LFI I++N + M + T + + F I+TFE+ +K++A G P Y + WN DF+++ L+ V T D+ GNL+ LRTFR+LR K + L TI+G + ++ NL
Sbjct: 109 KGKDIF--RFSASKAMWMLDP-FNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPT-------VESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYV--TMGIDL----GNLAALRTFRVLRALKTVA---IVPGLKTIVGAVIESVKNL 260 HSP 7 Score: 67.3958 bits (163), Expect = 1.465e-10 Identity = 51/213 (23.94%), Postives = 100/213 (46.95%), Query Frame = 2
Query: 3359 DINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFK--IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD 3991
D +E F ++ + + I F V +CC W W++ + IV F E FI + IV +++ + ++ + K ++ +L+ + FT F +E +K A+ YF++ W DF IV ++L+ G +R+ R LR + W + ++++ + + + ++ N+ V + IF+++GMQLFG + D
Sbjct: 740 DDDEDGPTFKDKALEVILKGIDVFCV--WDCC--------WVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDM--NKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQG-LSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKD 939 HSP 8 Score: 64.3142 bits (155), Expect = 1.396e-9 Identity = 53/197 (26.90%), Postives = 94/197 (47.72%), Query Frame = 2
Query: 4490 VIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALR-WYYFKIPWNVFDFIIVIFSI---------GFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSY------GIDATFNFETFGKAMIILFQISTSGGW 5032
+ +C I++NT+ M ++ H KE+ L N F FA+E +K++A+ YYF+ WN+FDFIIV S+ G V+ F + L KS + L+ + ++ AL N+ +L +I+FI+A++G+ +F + + + G +NF F + +I+F++ G W
Sbjct: 792 ITLC-IVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFK-----------------LAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRV-LCGEW 969 HSP 9 Score: 61.6178 bits (148), Expect = 9.746e-9 Identity = 85/330 (25.76%), Postives = 143/330 (43.33%), Query Frame = 2
Query: 584 SPF----NPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESEN-----------VETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIV-----IGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTC-NEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYW-----------ENLYQLIIRTNGDVHM-IYFMMVIFFGSFYLINVILSIV 1474
SPF LR +++ + F V+T I+++ +A+ ++ IFT I+ E +IK LA G VY FT +AW WLDF++ I LA + + ++R+LR LR L+ V+ GMK VV++L+ AI + + L++ + IFA++G+Q++ G KC + N H N ++ C +E Y + + +FD+ G + LC F++ T W E Q I TN ++M +YF+ I FGSF+ +N+ + ++
Sbjct: 1261 SPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVY-FT---NAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYF-KCEDM---------------------------------NGTKLSHEIIPN---RNACESENYTWVNS--------AMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETN--IYMYLYFVFFIIFGSFFTLNLFIGVI 1539 HSP 10 Score: 59.3066 bits (142), Expect = 4.659e-8 Identity = 45/153 (29.41%), Postives = 83/153 (54.25%), Query Frame = 2
Query: 2390 MIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTA----SKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCD 2836
++Y M+++ F VIF E +K +A G ++YF+ W DF+IV+LS++ L A + D+ A + +RT R LR + W+ M ++ + + + +I N+ +V I IFA++G+Q+F +Y + ++ + IP N C+
Sbjct: 1314 ILYYMDRI---FTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRS----MRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACE 1459
BLAST of Sodium channel protein vs. Ensembl Fly
Match: para (gene:FBgn0285944 transcript:FBtr0290121) HSP 1 Score: 723.776 bits (1867), Expect = 0.000e+0 Identity = 393/762 (51.57%), Postives = 534/762 (70.08%), Query Frame = 2
Query: 3374 EVIFWNQPYDCLPDSI-RCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVF----DAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKK----KCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPM---MDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKK 5623
E I P DC PDS + F + + +S + W +R F+++++K+FET ++ MI+ SSL L LED LP R L+DIL +MD FTVIF +EM++KW A+G YF +AWCWLDF IV+++LI+ V+ D AF+++RTLRALRPLRAVSRW+GMK+VVNAL+QAIPSIFNVLLVCL+FWLIF+I+G+QLF GK+FKC D N +L ++ N++ C ++EN +WVNS +NFD+V N YL LFQ+ATFKGW IM DA+D EV+ QP N + Y+YFV FIIFG+FFTLNLFIGVIIDNFN QKKK GGSLE+FMT+ QK+YY+AMKKM S P K +PRP+ I + I+ ++KFDI+IM FI LN M L+ + + LD++N +FV+IF+ EC++KI ALR++YF PWN+FD ++VI SI V+ + VSPTL+RV+RV ++GRVLRLVK A+GIRTLLF+L +SLPALFNI LLL L+MFI+AI G+S F V GI+ +NF+TFG++MI+LFQ+STS GW+ +LD ++N + + K CGS + + +L+SYLVISFL++INMYIAVILEN+SQAT+DVQ GLT DDYD++YE W+++DP T ++ LSEF+ + PL I +PNK ++ ++IPIC+G + CVD++D LTK+F GNP+ ++ + T +S+T QRE + AR I R+ AR +
Sbjct: 1236 EDILDEYPADCCPDSYYKKFPILAGD--DDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNAC----ESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFAR--KGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGE 1989 HSP 2 Score: 333.954 bits (855), Expect = 6.738e-92 Identity = 187/372 (50.27%), Postives = 253/372 (68.01%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSES--ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFD-NSSIGAETYENDSILLWNIKMDYYN-NPENW---QNPITF--CHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
D LE G +P L FP + A LED+DP+Y++ TFVV++ K IFRFS++KA ++ PFNP+R AI IL H LFS F++TTI++NCI M + E+ E IFT IYT E +K +ARG I+ FTYLRDAWNWLDF+VI LAY+ + LG L+ALR+ RVLRALKTV +PG+KT+V +++ ++ LRD ++LT+F LS+FAL+GLQ+Y G L KC+K F + S G T EN W DY+N N NW I+F C N + C+++Y C++ NPN+GYTSFD+FG + L AFRLMTQD+WE+LYQL++R G HM++F+++IF GSFYL+N+IL+IVAMSY
Sbjct: 65 DPTLEQGVPIPVRLQGSFPPE-LASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVT-MGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDEN-----W----DYHNRNSSNWYSEDEGISFPLCGNISG----AGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSY 421 HSP 3 Score: 259.61 bits (662), Expect = 4.561e-69 Identity = 168/309 (54.37%), Postives = 218/309 (70.55%), Query Frame = 2
Query: 2237 FCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKII 3163
FC W+CC + ++ VS IV DPF+ELFIT+CIV+NT+FM++D +D + M ++ N FF F EA MKL+A P+ YF E WN+FDF+IV LS++EL LS+LR+FRLLR+FKLAKSW T+NLL +I+GRT+GA+GNL VL II+FIFAV+GMQ+FG+ Y K++ FP +G +PRWNF DF HSFMIVFRVLCGE++ESMWDCM V + CIPFFLAT+++GNLV+LNLFLALLLS+F S SL ++ D NK+ EA R+ R WVK + + I KL I
Sbjct: 776 FCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGL-----EGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDR--FP--DGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADN-DTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQI 1074 HSP 4 Score: 78.5666 bits (192), Expect = 6.215e-14 Identity = 53/184 (28.80%), Postives = 96/184 (52.17%), Query Frame = 2
Query: 3470 TKAWWW------IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV 3988
+KA W IR A I+ H F FI+ I+ + + + +PT +E ++ FT I+ E +K A G Y +DAW WLDF ++ +A ++ D G A+RT R LR L+ V+ G+K +V A+I+++ ++ +V+++ + +F+++G+Q++ G KC+
Sbjct: 119 SKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILM-----IMPTTP----TVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTM--GIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCI 291 HSP 5 Score: 73.559 bits (179), Expect = 2.456e-12 Identity = 77/273 (28.21%), Postives = 137/273 (50.18%), Query Frame = 2
Query: 2126 NNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLL---RIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIP 2935
+N NE + KK +L +F E++ + + + +K P + + IV IV D ++ I + I LN M++D YD S + +++ +N F+VIF+ E +K+ A YF E WN+FD ++V+LSI+ L S I + +LLR R+ R+ +L K + + L + +L A+ N+C++L +++FIFA+ GM F E+ +G +NF F S +++F++ + + D + +N C P
Sbjct: 1544 DNFNE-QKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRW-RPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYH-YFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEK----------SGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAI-INEEACDP 1802 HSP 6 Score: 70.8626 bits (172), Expect = 1.499e-11 Identity = 50/171 (29.24%), Postives = 88/171 (51.46%), Query Frame = 2
Query: 2198 RKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYG----PRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNL 2698
+ +DI+ RF+ + W P ++ ++++ +I++ P LFI I++N + M + T + + F I+TFE+ +K++A G P Y + WN DF+++ L+ V T D+ GNL+ LRTFR+LR K + L TI+G + ++ NL
Sbjct: 109 KGKDIF--RFSASKAMWMLDP-FNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPT-------VESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYV--TMGIDL----GNLAALRTFRVLRALKTVA---IVPGLKTIVGAVIESVKNL 260 HSP 7 Score: 67.3958 bits (163), Expect = 1.719e-10 Identity = 44/179 (24.58%), Postives = 88/179 (49.16%), Query Frame = 2
Query: 3446 NCCSESCITKAWWWIRCSAFK--IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF 3976
+CC W W++ + IV F E FI + IV +++ + ++ + K ++ +L+ + FT F +E +K A+ YF++ W DF IV ++L+ G +R+ R LR + W + ++++ + + + ++ N+ V + IF+++GMQLFG +
Sbjct: 780 DCC--------WVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDM--NKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQG-LSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNY 947 HSP 8 Score: 62.3882 bits (150), Expect = 5.502e-9 Identity = 53/197 (26.90%), Postives = 93/197 (47.21%), Query Frame = 2
Query: 4490 VIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALR-WYYFKIPWNVFDFIIVIFSI---------GFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSY------GIDATFNFETFGKAMIILFQISTSGGW 5032
+ +C I++NT+ M ++ H KE+ L N F FA+E +K++A+ YYF+ WN+FDFIIV S+ G V+ F + L KS + L+ + ++ AL N+ +L +I+FI+A++G+ +F + + G +NF F + +I+F++ G W
Sbjct: 805 ITLC-IVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFK-----------------LAKSWPTLNLLISIMGRTVGALGNLTFVLCIIIFIFAVMGMQLFGKNYTDHKDRFPDGDLPRWNFTDFMHSFMIVFRV-LCGEW 982 HSP 9 Score: 61.2326 bits (147), Expect = 1.193e-8 Identity = 85/330 (25.76%), Postives = 143/330 (43.33%), Query Frame = 2
Query: 584 SPF----NPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESEN-----------VETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIV-----IGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTC-NEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYW-----------ENLYQLIIRTNGDVHM-IYFMMVIFFGSFYLINVILSIV 1474
SPF LR +++ + F V+T I+++ +A+ ++ IFT I+ E +IK LA G VY FT +AW WLDF++ I LA + + ++R+LR LR L+ V+ GMK VV++L+ AI + + L++ + IFA++G+Q++ G KC + N H N ++ C +E Y + + +FD+ G + LC F++ T W E Q I TN ++M +YF+ I FGSF+ +N+ + ++
Sbjct: 1264 SPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVY-FT---NAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYF-KCEDM---------------------------------NGTKLSHEIIPN---RNACESENYTWVNS--------AMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETN--IYMYLYFVFFIIFGSFFTLNLFIGVI 1542 HSP 10 Score: 59.3066 bits (142), Expect = 5.068e-8 Identity = 45/153 (29.41%), Postives = 83/153 (54.25%), Query Frame = 2
Query: 2390 MIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTA----SKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCD 2836
++Y M+++ F VIF E +K +A G ++YF+ W DF+IV+LS++ L A + D+ A + +RT R LR + W+ M ++ + + + +I N+ +V I IFA++G+Q+F +Y + ++ + IP N C+
Sbjct: 1317 ILYYMDRI---FTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRS----MRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACE 1462
BLAST of Sodium channel protein vs. Ensembl Fly
Match: para (gene:FBgn0285944 transcript:FBtr0303692) HSP 1 Score: 723.776 bits (1867), Expect = 0.000e+0 Identity = 393/762 (51.57%), Postives = 534/762 (70.08%), Query Frame = 2
Query: 3374 EVIFWNQPYDCLPDSI-RCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVF----DAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKK----KCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPM---MDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKK 5623
E I P DC PDS + F + + +S + W +R F+++++K+FET ++ MI+ SSL L LED LP R L+DIL +MD FTVIF +EM++KW A+G YF +AWCWLDF IV+++LI+ V+ D AF+++RTLRALRPLRAVSRW+GMK+VVNAL+QAIPSIFNVLLVCL+FWLIF+I+G+QLF GK+FKC D N +L ++ N++ C ++EN +WVNS +NFD+V N YL LFQ+ATFKGW IM DA+D EV+ QP N + Y+YFV FIIFG+FFTLNLFIGVIIDNFN QKKK GGSLE+FMT+ QK+YY+AMKKM S P K +PRP+ I + I+ ++KFDI+IM FI LN M L+ + + LD++N +FV+IF+ EC++KI ALR++YF PWN+FD ++VI SI V+ + VSPTL+RV+RV ++GRVLRLVK A+GIRTLLF+L +SLPALFNI LLL L+MFI+AI G+S F V GI+ +NF+TFG++MI+LFQ+STS GW+ +LD ++N + + K CGS + + +L+SYLVISFL++INMYIAVILEN+SQAT+DVQ GLT DDYD++YE W+++DP T ++ LSEF+ + PL I +PNK ++ ++IPIC+G + CVD++D LTK+F GNP+ ++ + T +S+T QRE + AR I R+ AR +
Sbjct: 1219 EDILDEYPADCCPDSYYKKFPILAGD--DDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNAC----ESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFAR--KGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGE 1972 HSP 2 Score: 333.954 bits (855), Expect = 6.169e-92 Identity = 187/372 (50.27%), Postives = 253/372 (68.01%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSES--ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFD-NSSIGAETYENDSILLWNIKMDYYN-NPENW---QNPITF--CHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
D LE G +P L FP + A LED+DP+Y++ TFVV++ K IFRFS++KA ++ PFNP+R AI IL H LFS F++TTI++NCI M + E+ E IFT IYT E +K +ARG I+ FTYLRDAWNWLDF+VI LAY+ + LG L+ALR+ RVLRALKTV +PG+KT+V +++ ++ LRD ++LT+F LS+FAL+GLQ+Y G L KC+K F + S G T EN W DY+N N NW I+F C N + C+++Y C++ NPN+GYTSFD+FG + L AFRLMTQD+WE+LYQL++R G HM++F+++IF GSFYL+N+IL+IVAMSY
Sbjct: 76 DPTLEQGVPIPVRLQGSFPPE-LASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVT-MGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDEN-----W----DYHNRNSSNWYSEDEGISFPLCGNISG----AGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSY 432 HSP 3 Score: 260.381 bits (664), Expect = 2.428e-69 Identity = 168/309 (54.37%), Postives = 218/309 (70.55%), Query Frame = 2
Query: 2237 FCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKII 3163
FC W+CC + ++ VS IV DPF+ELFIT+CIV+NT+FM++D +D + M ++ N FF F EA MKL+A P+ YF E WN+FDF+IV LS++EL LS+LR+FRLLR+FKLAKSW T+NLL +I+GRT+GA+GNL VL II+FIFAV+GMQ+FG+ Y K++ FP +G +PRWNF DF HSFMIVFRVLCGE++ESMWDCM V + CIPFFLAT+++GNLV+LNLFLALLLS+F S SL ++ D NK+ EA R+ R WVK + + I KL I
Sbjct: 774 FCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGL-----EGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDR--FP--DGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADN-DTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQI 1072 HSP 4 Score: 78.5666 bits (192), Expect = 6.923e-14 Identity = 53/184 (28.80%), Postives = 96/184 (52.17%), Query Frame = 2
Query: 3470 TKAWWW------IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV 3988
+KA W IR A I+ H F FI+ I+ + + + +PT +E ++ FT I+ E +K A G Y +DAW WLDF ++ +A ++ D G A+RT R LR L+ V+ G+K +V A+I+++ ++ +V+++ + +F+++G+Q++ G KC+
Sbjct: 130 SKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILM-----IMPTTP----TVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTM--GIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCI 302 HSP 5 Score: 73.559 bits (179), Expect = 2.352e-12 Identity = 77/273 (28.21%), Postives = 137/273 (50.18%), Query Frame = 2
Query: 2126 NNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLL---RIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIP 2935
+N NE + KK +L +F E++ + + + +K P + + IV IV D ++ I + I LN M++D YD S + +++ +N F+VIF+ E +K+ A YF E WN+FD ++V+LSI+ L S I + +LLR R+ R+ +L K + + L + +L A+ N+C++L +++FIFA+ GM F E+ +G +NF F S +++F++ + + D + +N C P
Sbjct: 1527 DNFNE-QKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRW-RPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYH-YFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEK----------SGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAI-INEEACDP 1785 HSP 6 Score: 70.4774 bits (171), Expect = 1.656e-11 Identity = 50/171 (29.24%), Postives = 88/171 (51.46%), Query Frame = 2
Query: 2198 RKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYG----PRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNL 2698
+ +DI+ RF+ + W P ++ ++++ +I++ P LFI I++N + M + T + + F I+TFE+ +K++A G P Y + WN DF+++ L+ V T D+ GNL+ LRTFR+LR K + L TI+G + ++ NL
Sbjct: 120 KGKDIF--RFSASKAMWMLDP-FNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPT-------VESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYV--TMGIDL----GNLAALRTFRVLRALKTVA---IVPGLKTIVGAVIESVKNL 271 HSP 7 Score: 67.3958 bits (163), Expect = 1.592e-10 Identity = 51/213 (23.94%), Postives = 100/213 (46.95%), Query Frame = 2
Query: 3359 DINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFK--IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD 3991
D +E F ++ + + I F V +CC W W++ + IV F E FI + IV +++ + ++ + K ++ +L+ + FT F +E +K A+ YF++ W DF IV ++L+ G +R+ R LR + W + ++++ + + + ++ N+ V + IF+++GMQLFG + D
Sbjct: 751 DDDEDGPTFKDKALEVILKGIDVFCV--WDCC--------WVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDM--NKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQG-LSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKD 950 HSP 8 Score: 64.3142 bits (155), Expect = 1.431e-9 Identity = 53/197 (26.90%), Postives = 94/197 (47.72%), Query Frame = 2
Query: 4490 VIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALR-WYYFKIPWNVFDFIIVIFSI---------GFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSY------GIDATFNFETFGKAMIILFQISTSGGW 5032
+ +C I++NT+ M ++ H KE+ L N F FA+E +K++A+ YYF+ WN+FDFIIV S+ G V+ F + L KS + L+ + ++ AL N+ +L +I+FI+A++G+ +F + + + G +NF F + +I+F++ G W
Sbjct: 803 ITLC-IVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFK-----------------LAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRV-LCGEW 980 HSP 9 Score: 61.6178 bits (148), Expect = 9.986e-9 Identity = 85/330 (25.76%), Postives = 146/330 (44.24%), Query Frame = 2
Query: 584 SPF----NPLRAAAIRILTHSLFSFFVLTTIIINCIAMT-------KNSESENV----ETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIV-----IGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTC-NEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYW-----------ENLYQLIIRTNGDVHM-IYFMMVIFFGSFYLINVILSIV 1474
SPF LR +++ + F V+T I+++ +A+ + +++ + IFT I+ E +IK LA G VY FT +AW WLDF++ I LA + + ++R+LR LR L+ V+ GMK VV++L+ AI + + L++ + IFA++G+Q++ G KC + N H N ++ C +E Y + + +FD+ G + LC F++ T W E Q I TN ++M +YF+ I FGSF+ +N+ + ++
Sbjct: 1247 SPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVY-FT---NAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYF-KCEDM---------------------------------NGTKLSHEIIPN---RNACESENYTWVNS--------AMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETN--IYMYLYFVFFIIFGSFFTLNLFIGVI 1525 HSP 10 Score: 59.3066 bits (142), Expect = 5.149e-8 Identity = 45/153 (29.41%), Postives = 83/153 (54.25%), Query Frame = 2
Query: 2390 MIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTA----SKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCD 2836
++Y M+++ F VIF E +K +A G ++YF+ W DF+IV+LS++ L A + D+ A + +RT R LR + W+ M ++ + + + +I N+ +V I IFA++G+Q+F +Y + ++ + IP N C+
Sbjct: 1300 ILYYMDRI---FTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRS----MRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACE 1445
BLAST of Sodium channel protein vs. Ensembl Fly
Match: para (gene:FBgn0285944 transcript:FBtr0303701) HSP 1 Score: 723.391 bits (1866), Expect = 0.000e+0 Identity = 393/762 (51.57%), Postives = 534/762 (70.08%), Query Frame = 2
Query: 3374 EVIFWNQPYDCLPDSI-RCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVF----DAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKK----KCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPM---MDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKK 5623
E I P DC PDS + F + + +S + W +R F+++++K+FET ++ MI+ SSL L LED LP R L+DIL +MD FTVIF +EM++KW A+G YF +AWCWLDF IV+++LI+ V+ D AF+++RTLRALRPLRAVSRW+GMK+VVNAL+QAIPSIFNVLLVCL+FWLIF+I+G+QLF GK+FKC D N +L ++ N++ C ++EN +WVNS +NFD+V N YL LFQ+ATFKGW IM DA+D EV+ QP N + Y+YFV FIIFG+FFTLNLFIGVIIDNFN QKKK GGSLE+FMT+ QK+YY+AMKKM S P K +PRP+ I + I+ ++KFDI+IM FI LN M L+ + + LD++N +FV+IF+ EC++KI ALR++YF PWN+FD ++VI SI V+ + VSPTL+RV+RV ++GRVLRLVK A+GIRTLLF+L +SLPALFNI LLL L+MFI+AI G+S F V GI+ +NF+TFG++MI+LFQ+STS GW+ +LD ++N + + K CGS + + +L+SYLVISFL++INMYIAVILEN+SQAT+DVQ GLT DDYD++YE W+++DP T ++ LSEF+ + PL I +PNK ++ ++IPIC+G + CVD++D LTK+F GNP+ ++ + T +S+T QRE + AR I R+ AR +
Sbjct: 1254 EDILDEYPADCCPDSYYKKFPILAGD--DDSPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNAC----ESENYTWVNSAMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETNIYMYLYFVFFIIFGSFFTLNLFIGVIIDNFNEQKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRWRPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYHYFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEKSGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAIINEEACDPPDNDKGYPGNCGSATVGITFLLSYLVISFLIVINMYIAVILENYSQATEDVQEGLTDDDYDMYYEIWQQFDPEGTQYIRYDQLSEFLDVLEPPLQIHKPNKYKIISMDIPICRGDLMYCVDILDALTKDFFAR--KGNPIEETGEIGEIAARPDTEGYEPVSSTLWRQREEYCARLIQHAWRKHKARGE 2007 HSP 2 Score: 333.569 bits (854), Expect = 7.684e-92 Identity = 187/372 (50.27%), Postives = 253/372 (68.01%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSES--ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFD-NSSIGAETYENDSILLWNIKMDYYN-NPENW---QNPITF--CHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
D LE G +P L FP + A LED+DP+Y++ TFVV++ K IFRFS++KA ++ PFNP+R AI IL H LFS F++TTI++NCI M + E+ E IFT IYT E +K +ARG I+ FTYLRDAWNWLDF+VI LAY+ + LG L+ALR+ RVLRALKTV +PG+KT+V +++ ++ LRD ++LT+F LS+FAL+GLQ+Y G L KC+K F + S G T EN W DY+N N NW I+F C N + C+++Y C++ NPN+GYTSFD+FG + L AFRLMTQD+WE+LYQL++R G HM++F+++IF GSFYL+N+IL+IVAMSY
Sbjct: 65 DPTLEQGVPIPVRLQGSFPPE-LASTPLEDIDPYYSNVLTFVVVSKGKDIFRFSASKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPTVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVT-MGIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCIKKFPLDGSWGNLTDEN-----W----DYHNRNSSNWYSEDEGISFPLCGNISG----AGQCDDDYVCLQGFGPNPNYGYTSFDSFGWAFLSAFRLMTQDFWEDLYQLVLRAAGPWHMLFFIVIIFLGSFYLVNLILAIVAMSY 421 HSP 3 Score: 260.381 bits (664), Expect = 2.866e-69 Identity = 168/309 (54.37%), Postives = 218/309 (70.55%), Query Frame = 2
Query: 2237 FCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKII 3163
FC W+CC + ++ VS IV DPF+ELFIT+CIV+NT+FM++D +D + M ++ N FF F EA MKL+A P+ YF E WN+FDF+IV LS++EL LS+LR+FRLLR+FKLAKSW T+NLL +I+GRT+GA+GNL VL II+FIFAV+GMQ+FG+ Y K++ FP +G +PRWNF DF HSFMIVFRVLCGE++ESMWDCM V + CIPFFLAT+++GNLV+LNLFLALLLS+F S SL ++ D NK+ EA R+ R WVK + + I KL I
Sbjct: 784 FCVWDCCWVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGL-----EGVQGLSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDR--FP--DGDLPRWNFTDFMHSFMIVFRVLCGEWIESMWDCMYVGDVSCIPFFLATVVIGNLVVLNLFLALLLSNFGSSSLSAPTADN-DTNKIAEAFNRIGRFKSWVKRNIADCFKLIRNKLTNQI 1082 HSP 4 Score: 78.5666 bits (192), Expect = 6.384e-14 Identity = 53/184 (28.80%), Postives = 96/184 (52.17%), Query Frame = 2
Query: 3470 TKAWWW------IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV 3988
+KA W IR A I+ H F FI+ I+ + + + +PT +E ++ FT I+ E +K A G Y +DAW WLDF ++ +A ++ D G A+RT R LR L+ V+ G+K +V A+I+++ ++ +V+++ + +F+++G+Q++ G KC+
Sbjct: 119 SKAMWMLDPFNPIRRVAIYILVHPLFSLFIITTILVNCILM-----IMPTTP----TVESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYVTM--GIDLGNLAALRTFRVLRALKTVAIVPGLKTIVGAVIESVKNLRDVIILTMFSLSVFALMGLQIYMGVLTQKCI 291 HSP 5 Score: 73.1738 bits (178), Expect = 2.653e-12 Identity = 77/273 (28.21%), Postives = 137/273 (50.18%), Query Frame = 2
Query: 2126 NNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLL---RIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIP 2935
+N NE + KK +L +F E++ + + + +K P + + IV IV D ++ I + I LN M++D YD S + +++ +N F+VIF+ E +K+ A YF E WN+FD ++V+LSI+ L S I + +LLR R+ R+ +L K + + L + +L A+ N+C++L +++FIFA+ GM F E+ +G +NF F S +++F++ + + D + +N C P
Sbjct: 1562 DNFNE-QKKKAGGSLEMFMTEDQKKYYNAMKKMGSKKPLKAIPRPRW-RPQAIVFEIVTDKKFDIIIMLFIGLNMFTMTLDRYDASDTYNAVLDYLNAIFVVIFSSECLLKIFALRYH-YFIEPWNLFDVVVVILSILGLVLSDIIEKYFVSPTLLRVVRVAKVGRVLRLVKGAKGIRTLLFALAMSLPALFNICLLLFLVMFIFAIFGMSFFMHVKEK----------SGINDVYNFKTFGQSMILLFQMSTSAGWDGVLDAI-INEEACDP 1820 HSP 6 Score: 70.8626 bits (172), Expect = 1.606e-11 Identity = 50/171 (29.24%), Postives = 88/171 (51.46%), Query Frame = 2
Query: 2198 RKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYG----PRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNL 2698
+ +DI+ RF+ + W P ++ ++++ +I++ P LFI I++N + M + T + + F I+TFE+ +K++A G P Y + WN DF+++ L+ V T D+ GNL+ LRTFR+LR K + L TI+G + ++ NL
Sbjct: 109 KGKDIF--RFSASKAMWMLDP-FNPIRRVAIYILVHPLFSLFIITTILVNCILMIMPTTPT-------VESTEVIFTGIYTFESAVKVMARGFILCPFTYLRDAWNWLDFVVIALAYV--TMGIDL----GNLAALRTFRVLRALKTVA---IVPGLKTIVGAVIESVKNL 260 HSP 7 Score: 67.3958 bits (163), Expect = 1.492e-10 Identity = 51/213 (23.94%), Postives = 100/213 (46.95%), Query Frame = 2
Query: 3359 DINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFK--IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD 3991
D +E F ++ + + I F V +CC W W++ + IV F E FI + IV +++ + ++ + K ++ +L+ + FT F +E +K A+ YF++ W DF IV ++L+ G +R+ R LR + W + ++++ + + + ++ N+ V + IF+++GMQLFG + D
Sbjct: 761 DDDEDGPTFKDKALEVILKGIDVFCV--WDCC--------WVWLKFQEWVSLIVFDPFVELFITLCIVVNTMFMAMDHHDM--NKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQG-LSVLRSFRLLRVFKLAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKD 960 HSP 8 Score: 64.3142 bits (155), Expect = 1.422e-9 Identity = 53/197 (26.90%), Postives = 94/197 (47.72%), Query Frame = 2
Query: 4490 VIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALR-WYYFKIPWNVFDFIIVIFSI---------GFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSY------GIDATFNFETFGKAMIILFQISTSGGW 5032
+ +C I++NT+ M ++ H KE+ L N F FA+E +K++A+ YYF+ WN+FDFIIV S+ G V+ F + L KS + L+ + ++ AL N+ +L +I+FI+A++G+ +F + + + G +NF F + +I+F++ G W
Sbjct: 813 ITLC-IVVNTMFMAMDHHDMNKEMERVLKSGNYFFTATFAIEATMKLMAMSPKYYFQEGWNIFDFIIVALSLLELGLEGVQGLSVLRSFRLLRVFK-----------------LAKSWPTLNLLISIMGRTMGALGNLTFVLCIIIFIFAVMGMQLFGKNYHDHKDRFPDGDLPRWNFTDFMHSFMIVFRV-LCGEW 990 HSP 9 Score: 61.2326 bits (147), Expect = 1.235e-8 Identity = 85/330 (25.76%), Postives = 143/330 (43.33%), Query Frame = 2
Query: 584 SPF----NPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESEN-----------VETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIV-----IGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTC-NEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYW-----------ENLYQLIIRTNGDVHM-IYFMMVIFFGSFYLINVILSIV 1474
SPF LR +++ + F V+T I+++ +A+ ++ IFT I+ E +IK LA G VY FT +AW WLDF++ I LA + + ++R+LR LR L+ V+ GMK VV++L+ AI + + L++ + IFA++G+Q++ G KC + N H N ++ C +E Y + + +FD+ G + LC F++ T W E Q I TN ++M +YF+ I FGSF+ +N+ + ++
Sbjct: 1282 SPFWQGWGNLRLKTFQLIENKYFETAVITMILMSSLALALEDVHLPQRPILQDILYYMDRIFTVIFFLEMLIKWLALGFKVY-FT---NAWCWLDFVIVMLSLINLAAVWSGADDVPAFRSMRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYF-KCEDM---------------------------------NGTKLSHEIIPN---RNACESENYTWVNS--------AMNFDHVGNAYLCLFQVATFKGWIQIMNDAIDSREVDKQPIRETN--IYMYLYFVFFIIFGSFFTLNLFIGVI 1560 HSP 10 Score: 58.9214 bits (141), Expect = 5.473e-8 Identity = 45/153 (29.41%), Postives = 83/153 (54.25%), Query Frame = 2
Query: 2390 MIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTA----SKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCD 2836
++Y M+++ F VIF E +K +A G ++YF+ W DF+IV+LS++ L A + D+ A + +RT R LR + W+ M ++ + + + +I N+ +V I IFA++G+Q+F +Y + ++ + IP N C+
Sbjct: 1335 ILYYMDRI---FTVIFFLEMLIKWLALGFKVYFTNAWCWLDFVIVMLSLINLAAVWSGADDVPAFRS----MRTLRALRPLRAVSRWEGMKVVVNALVQAIPSIFNVLLVCLIFWLIFAIMGVQLFAGKYFKCEDMNGTKLSHEIIPNRNACE 1480
BLAST of Sodium channel protein vs. Ensembl Zebrafish
Match: scn1lab (sodium channel, voltage-gated, type I like, alpha b [Source:ZFIN;Acc:ZDB-GENE-060906-1]) HSP 1 Score: 862.062 bits (2226), Expect = 0.000e+0 Identity = 544/1191 (45.68%), Postives = 755/1191 (63.39%), Query Frame = 2
Query: 2150 KKRISALSVF-----ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESN---------------------------PINIQAAIIKMNKNETEPSRNVHDPQLN---QVAF------NLWLENYEPK------------------------FDADPDINEAEVIFWNQPYDCLPDSIRCFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
K+ +S S+ E E +K +F +F W+C PA+ +K+I++ IV+DPF++L ITICIVLNT+FM+++ Y + ++++ NL F IFT E +K+IA P YF E WN+FD +IV LS++EL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y + ++ ++PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +D+S ++N LQ A+ R+ + I VK ST + L + R + P D S+ P N + +K E + +H+P L +A NL E++ + D P AE + +P + L D CF V CC E K+WW +R + F IV+H +FE+FI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G KYF +AWCWLDF IV V+L+S + A K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GK++ CV+ N L S+V NKSDCY L Q + W N +INFDNV GYL+L Q+ATFKGW DIM AVD ++ QP Y N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK+GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + +++I Q FDI+IM I LN + M +E+ Q +++ + L +N VF+++F ECV+K+I+LR Y+F I WNVFDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +M+ LFQI+TS GW+ +L ++N +C + + CG+ + + + VSY++I FL+++NMYIAVILENFS AT++ L++DD+++FYE WE +DP AT F+E LS+F + PL I +PNKI+L +++P+ G I C+D++ T LGE
Sbjct: 685 KRAMSVASILTNTMEELEESRQKCPPCWYKFANSFLIWDCSPAWLKVKEIMNMIVMDPFVDLAITICIVLNTLFMAMEHYPMTTQFSHLLSVGNLVFTGIFTAEMCLKVIALDPYYYFQEGWNIFDGIIVSLSLMELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYRECVCKI---SESCELPRWHMDDFFHSFLIVFRVLCGEWIETMWDCMEVAGQSMCLIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDS-EMNNLQIAVGRIQKGIALVK----STLRQFLQSLFLAGSKPRSLDEEKPLDDLHSDGKENCLSNHAPVAVDLTKDYLKEGCVSPGNGVESHVKYGIEEGDYMSFIHNPSLTVTVPIAVGESDFENLNTEDFSSESSDVEGSKEKLPVDTVLSSSEGSTVDIRPPGEGAESVEL-EPEESL-DPEACFTEGCVMRFQCCQVDVEKGKWKSWWILRKTCFIIVEHNWFESFIIFMILLSSGALAFEDIYIEQRKTIKTVLEYADKVFTYIFILEMLLKWVAYGFAKYFTNAWCWLDFLIVDVSLVSLVANALGYSELSAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYYCVNITNDELFPVSVVNNKSDCYALIQNNDTARWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRDLEQQPVYETNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGLVFDLITKQAFDIIIMILICLNMVTMMVETDDQTEDMDNILYWINLVFIILFTGECVLKMISLRHYFFTIGWNVFDFVVVILSIVGMFLSEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMLCLFQITTSAGWDGLLAPILNKQDPDCDSEMEHPGSFYRGNCGNPSVGIFFFVSYIIICFLIVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWERFDPNATQFVEYNKLSDFADALDPPLRIAKPNKIQLIAMDLPMVSGDRIHCLDILFAFTLRVLGE 1859 HSP 2 Score: 317.005 bits (811), Expect = 1.185e-86 Identity = 181/378 (47.88%), Postives = 251/378 (66.40%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESE---NVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNF-----------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
++ LEAGK LP GD P LEDLD +Y++++TF+V+N K IFRF++T A ++ SPFNPLR +IR+L HSLFSF ++ TI+ NC MT ++ E NVE FT IYT E +IK LARG V +FT+LRD WNWLDF VI +AY+ +VN LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCVK+ E D++ ++ ++ +N+ NW I+ +N+ N+ C E + CI+ NP++GYTSFD F + L FRLMTQD+WENLYQ +R G +MI+F++VIF GSFYL+N+IL++VAM+Y
Sbjct: 54 NRDLEAGKPLPFIYGDVPPH--MLSVPLEDLDTYYSNQKTFIVLNRGKAIFRFNATPALYILSPFNPLRRISIRVLVHSLFSFVIMCTILTNCAFMTLSNPPEWAKNVEYTFTGIYTFESLIKILARGFCVGKFTFLRDPWNWLDFSVILMAYVTEFVN--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRQKCVKM-------PEVNVTDNLTDYSTEL--HNSTFNWTEYISDSNNYYFIPGRRDALLCGNSSDAGQCPEGFTCIKA-GRNPDYGYTSFDTFSWAFLSLFRLMTQDFWENLYQQTLRAAGKPYMIFFVLVIFLGSFYLVNLILAVVAMAY 417 HSP 3 Score: 84.7297 bits (208), Expect = 1.287e-15 Identity = 51/188 (27.13%), Postives = 102/188 (54.26%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLG-FTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVDSNNVRLHESLVQNKSDCY 4045
+R + +++ H F I+ I+ + +TL + +E+ G +T L++++ + F +G + +D W WLDF+++L+A ++ F + G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV V + ++L ++ +
Sbjct: 119 LRRISIRVLVHSLFSFVIMCTILTNCAFMTLSNP-----PEWAKNVEYTFTGIYTFESLIKILARGFCVGKFTFLRDPWNWLDFSVILMAYVTEF--VNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRQKCVKMPEVNVTDNLTDYSTELH 299 HSP 4 Score: 66.6254 bits (161), Expect = 3.332e-10 Identity = 78/319 (24.45%), Postives = 144/319 (45.14%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALG-----KXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLY-----------QLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIV 1474
LR I+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G Y +AW WLDF+++ ++ + V +ALG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G + CV + ND + P + N + C+ NN + R N N FDN G L ++ T W ++ Q + TN +++ YF++ I FGSF+ +N+ + ++
Sbjct: 1188 LRKTCFIIVEHNWFESFIIFMILLSSGALAFEDIYIEQRKTIKTVLEYADKVFTYIFILEMLLKWVAYGFA----KYFTNAWCWLDFLIVDVSLVSLVANALGYSELSAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYY-CVNI-----------TNDELF-----------PVSVVNNKSDCYALIQNN----------DTARWKNVKIN-----FDNVGAGYLALLQVATFKGWMDIMYAAVDSRDLEQQPVYETNLYMYL-YFVIFIIFGSFFTLNLFIGVI 1463 HSP 5 Score: 65.4698 bits (158), Expect = 7.730e-10 Identity = 54/188 (28.72%), Postives = 94/188 (50.00%), Query Frame = 2
Query: 2144 EMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLL---RIFKLAKSWQTMNLLFTIIGRTLGAIGNL 2698
+ KK++ +F E + + + + +K PA + + I F ++ I I I LN V M ++T D + M I+ +NL F+++FT E +K+I+ +F+ WNVFDF++V+LSIV + S+ I + +L R RL RI +L K + + L + +L A+ N+
Sbjct: 1469 QQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGLVFDLITKQAF-DIIIMILICLNMVTMMVETDDQTEDMDNILYWINLVFIILFTGECVLKMISLR-HYFFTIGWNVFDFVVVILSIVGMFLSEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNI 1654
BLAST of Sodium channel protein vs. Ensembl Zebrafish
Match: scn1lab (sodium channel, voltage-gated, type I like, alpha b [Source:ZFIN;Acc:ZDB-GENE-060906-1]) HSP 1 Score: 862.062 bits (2226), Expect = 0.000e+0 Identity = 544/1191 (45.68%), Postives = 755/1191 (63.39%), Query Frame = 2
Query: 2150 KKRISALSVF-----ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESN---------------------------PINIQAAIIKMNKNETEPSRNVHDPQLN---QVAF------NLWLENYEPK------------------------FDADPDINEAEVIFWNQPYDCLPDSIRCFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
K+ +S S+ E E +K +F +F W+C PA+ +K+I++ IV+DPF++L ITICIVLNT+FM+++ Y + ++++ NL F IFT E +K+IA P YF E WN+FD +IV LS++EL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y + ++ ++PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +D+S ++N LQ A+ R+ + I VK ST + L + R + P D S+ P N + +K E + +H+P L +A NL E++ + D P AE + +P + L D CF V CC E K+WW +R + F IV+H +FE+FI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G KYF +AWCWLDF IV V+L+S + A K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GK++ CV+ N L S+V NKSDCY L Q + W N +INFDNV GYL+L Q+ATFKGW DIM AVD ++ QP Y N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK+GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + +++I Q FDI+IM I LN + M +E+ Q +++ + L +N VF+++F ECV+K+I+LR Y+F I WNVFDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +M+ LFQI+TS GW+ +L ++N +C + + CG+ + + + VSY++I FL+++NMYIAVILENFS AT++ L++DD+++FYE WE +DP AT F+E LS+F + PL I +PNKI+L +++P+ G I C+D++ T LGE
Sbjct: 685 KRAMSVASILTNTMEELEESRQKCPPCWYKFANSFLIWDCSPAWLKVKEIMNMIVMDPFVDLAITICIVLNTLFMAMEHYPMTTQFSHLLSVGNLVFTGIFTAEMCLKVIALDPYYYFQEGWNIFDGIIVSLSLMELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYRECVCKI---SESCELPRWHMDDFFHSFLIVFRVLCGEWIETMWDCMEVAGQSMCLIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDS-EMNNLQIAVGRIQKGIALVK----STLRQFLQSLFLAGSKPRSLDEEKPLDDLHSDGKENCLSNHAPVAVDLTKDYLKEGCVSPGNGVESHVKYGIEEGDYMSFIHNPSLTVTVPIAVGESDFENLNTEDFSSESSDVEGSKEKLPVDTVLSSSEGSTVDIRPPGEGAESVEL-EPEESL-DPEACFTEGCVMRFQCCQVDVEKGKWKSWWILRKTCFIIVEHNWFESFIIFMILLSSGALAFEDIYIEQRKTIKTVLEYADKVFTYIFILEMLLKWVAYGFAKYFTNAWCWLDFLIVDVSLVSLVANALGYSELSAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYYCVNITNDELFPVSVVNNKSDCYALIQNNDTARWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRDLEQQPVYETNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGLVFDLITKQAFDIIIMILICLNMVTMMVETDDQTEDMDNILYWINLVFIILFTGECVLKMISLRHYFFTIGWNVFDFVVVILSIVGMFLSEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREAGIDDMFNFETFGNSMLCLFQITTSAGWDGLLAPILNKQDPDCDSEMEHPGSFYRGNCGNPSVGIFFFVSYIIICFLIVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWERFDPNATQFVEYNKLSDFADALDPPLRIAKPNKIQLIAMDLPMVSGDRIHCLDILFAFTLRVLGE 1859 HSP 2 Score: 317.005 bits (811), Expect = 1.185e-86 Identity = 181/378 (47.88%), Postives = 251/378 (66.40%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESE---NVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNF-----------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
++ LEAGK LP GD P LEDLD +Y++++TF+V+N K IFRF++T A ++ SPFNPLR +IR+L HSLFSF ++ TI+ NC MT ++ E NVE FT IYT E +IK LARG V +FT+LRD WNWLDF VI +AY+ +VN LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCVK+ E D++ ++ ++ +N+ NW I+ +N+ N+ C E + CI+ NP++GYTSFD F + L FRLMTQD+WENLYQ +R G +MI+F++VIF GSFYL+N+IL++VAM+Y
Sbjct: 54 NRDLEAGKPLPFIYGDVPPH--MLSVPLEDLDTYYSNQKTFIVLNRGKAIFRFNATPALYILSPFNPLRRISIRVLVHSLFSFVIMCTILTNCAFMTLSNPPEWAKNVEYTFTGIYTFESLIKILARGFCVGKFTFLRDPWNWLDFSVILMAYVTEFVN--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRQKCVKM-------PEVNVTDNLTDYSTEL--HNSTFNWTEYISDSNNYYFIPGRRDALLCGNSSDAGQCPEGFTCIKA-GRNPDYGYTSFDTFSWAFLSLFRLMTQDFWENLYQQTLRAAGKPYMIFFVLVIFLGSFYLVNLILAVVAMAY 417 HSP 3 Score: 84.7297 bits (208), Expect = 1.287e-15 Identity = 51/188 (27.13%), Postives = 102/188 (54.26%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLG-FTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVDSNNVRLHESLVQNKSDCY 4045
+R + +++ H F I+ I+ + +TL + +E+ G +T L++++ + F +G + +D W WLDF+++L+A ++ F + G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV V + ++L ++ +
Sbjct: 119 LRRISIRVLVHSLFSFVIMCTILTNCAFMTLSNP-----PEWAKNVEYTFTGIYTFESLIKILARGFCVGKFTFLRDPWNWLDFSVILMAYVTEF--VNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRQKCVKMPEVNVTDNLTDYSTELH 299 HSP 4 Score: 66.6254 bits (161), Expect = 3.332e-10 Identity = 78/319 (24.45%), Postives = 144/319 (45.14%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALG-----KXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLY-----------QLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIV 1474
LR I+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G Y +AW WLDF+++ ++ + V +ALG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G + CV + ND + P + N + C+ NN + R N N FDN G L ++ T W ++ Q + TN +++ YF++ I FGSF+ +N+ + ++
Sbjct: 1188 LRKTCFIIVEHNWFESFIIFMILLSSGALAFEDIYIEQRKTIKTVLEYADKVFTYIFILEMLLKWVAYGFA----KYFTNAWCWLDFLIVDVSLVSLVANALGYSELSAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYY-CVNI-----------TNDELF-----------PVSVVNNKSDCYALIQNN----------DTARWKNVKIN-----FDNVGAGYLALLQVATFKGWMDIMYAAVDSRDLEQQPVYETNLYMYL-YFVIFIIFGSFFTLNLFIGVI 1463 HSP 5 Score: 65.4698 bits (158), Expect = 7.730e-10 Identity = 54/188 (28.72%), Postives = 94/188 (50.00%), Query Frame = 2
Query: 2144 EMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLL---RIFKLAKSWQTMNLLFTIIGRTLGAIGNL 2698
+ KK++ +F E + + + + +K PA + + I F ++ I I I LN V M ++T D + M I+ +NL F+++FT E +K+I+ +F+ WNVFDF++V+LSIV + S+ I + +L R RL RI +L K + + L + +L A+ N+
Sbjct: 1469 QQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGLVFDLITKQAF-DIIIMILICLNMVTMMVETDDQTEDMDNILYWINLVFIILFTGECVLKMISLR-HYFFTIGWNVFDFVVVILSIVGMFLSEMIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNI 1654
BLAST of Sodium channel protein vs. Ensembl Zebrafish
Match: scn1laa (sodium channel, voltage-gated, type I-like, alpha [Source:ZFIN;Acc:ZDB-GENE-040426-751]) HSP 1 Score: 860.907 bits (2223), Expect = 0.000e+0 Identity = 549/1168 (47.00%), Postives = 741/1168 (63.44%), Query Frame = 2
Query: 2156 RISALSVFECENKLRK-RDIYLQR-------FNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNH--IGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMN-KNETE-----PSRNVHDP---------QLNQVAFNLWLENYEPKFDADPDINEA--------------------EVIFWNQPYDCLPDSIRCFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD-SNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKK--------KCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
R ALSV N + +I+ QR F + + W+C P + +K++V IV+DPF +LFITICIV+NTVFM+++ Y +++ N+ F+ IFT E K+IA P YF E WN+FD +IV LS++EL + +S + LR FRLLR+FKLAKSW T+N+L IIG +LGA+ NL +VLAIIVFIFAVVGMQ+FG+ Y + + +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +E D N LQ A+ R+ + ++K L S + + KK K + + DS I + + + ++TE PS V P LN F+ + E + D+ + E++ + Y P+ CF VR CC E I K WW +R + + IV+H +FETFI+ MI+ SS L ED +L TRK +K ILEF D FT IF++EM+LKW A G KYF + WCWLDF IV V+LIS + A K++RTLRA RPLRA+SR+DGM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ F GKF +C++ + RL S V NK+DC + K V W N INFDNV NGYL+L Q+ATFKGW IM AVD QP+Y N + Y+YFV+FII GAFFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRPK+ I + ++++ Q FDI IM I LN + M LE+ Q E++ AL +N +F+++F ECV+K+IALR Y+F + WNVFDFI+VI SI + + + VSPTL RVIR+ RIGRVLRL++SA+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V + GID FNFETF +M+ LFQI+TSGGW+ +L ++N+ +C K CG+ + + + VSY++I FL+++NMYIAVILENFS AT++ L++DD+D FYE WE++DP AT FME LS+F + PL IP+PN+ +L +++P+ G I C+D++ TK LGE
Sbjct: 674 RERALSVASGSNNPAECSEIFKQRCEEWWFEFAENYLIWDCSPTWLRIKEVVHMIVMDPFADLFITICIVINTVFMAMECYPMDGPYSSMLSTANVVFIGIFTAEMIFKVIALDPYSYFLEGWNIFDSIIVTLSLMELCLANVLSG----IGFLRPFRLLRVFKLAKSWPTLNMLIKIIGNSLGALSNLTLVLAIIVFIFAVVGMQLFGKSYGECVCKIS---SDCMLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQPLCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAVSGEE--DENNLQIAVRRIRKGFTFIKTCLQSFFRSGCLRKKKQKSADGNMIVNCKERKDSTNHTTIELVKSTGGTDLDSDTENFISNPSLTVSVPIAAEESDFECLNTEDFSSLSSDLEEDKEKLSDVASSFSEGSTVDLRPLGKDGEDALELVLEEEAY---PEP--CFTAGCVRRFRCCQVDEEKGIWKKWWLLRQTCYNIVEHSWFETFIVFMILLSSGALAFEDIYLDTRKSVKTILEFADKVFTYIFILEMLLKWVAYGFAKYFTNLWCWLDFVIVDVSLISLVANALNYSELSAIKSLRTLRAFRPLRALSRFDGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIVGVNFFAGKFSECINITTEQRLPVSEVYNKTDCERMGK--GVRWRNVIINFDNVGNGYLALLQVATFKGWMPIMYAAVDSRNPEEQPEYEVNLYMYLYFVIFIILGAFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPKNKIQGLIFDLVTKQAFDIFIMVLIWLNMVTMMLETEDQSIEMTRALYWINVIFIVLFTTECVLKMIALRHYFFTVGWNVFDFIVVILSIVGMSLTEVIKKYFVSPTLFRVIRLARIGRVLRLIRSAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKTAGIDDMFNFETFPNSMLCLFQITTSGGWDGLLAPMLNSKPPDCDPTKNFTGSSVVGDCGNPSIGIFFFVSYIIICFLIVVNMYIAVILENFSVATEESAEPLSEDDFDRFYEVWEKFDPKATQFMEYGKLSDFADSLDPPLRIPKPNRFQLMSMDLPMVTGDRIHCLDILFAFTKRVLGE 1824 HSP 2 Score: 300.827 bits (769), Expect = 1.252e-81 Identity = 172/369 (46.61%), Postives = 243/369 (65.85%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAM--TKNSE-SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPEN-----WQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP G+ K LEDLD FY++ TF+V++ K I+RF++T A ++FS FN LR +I+IL H LF+F ++ TI+ NC M T+ + +++VE +FT IYT E ++K ARG V +FT+LRD WNWLDFIVI +AY+ +V+ LG +SALR+ RVLRALKT++ +PG+KT+V +L+ ++ KL D ++LT+F LS+FAL+GLQ++ G L KCV + Y+N + DY NNP N + C N + C E Y C++ N NP++GYTSFD+FG + L FRLMTQD+WENLYQ +R G +MI+F++VIF GSFYL+N+IL++VAM+Y
Sbjct: 56 LEAGKTLPFIYGEV--PKGLVSTPLEDLDQFYSNHTTFIVVSKKKEIYRFNATPALYIFSSFNLLRRISIQILVHFLFNFIIICTILANCAFMVYTQTPDWAKSVEYVFTGIYTLEALVKITARGFCVGRFTFLRDPWNWLDFIVIVMAYVTEFVD--LGSVSALRAFRVLRALKTISVVPGLKTIVGALIQSVKKLSDVMILTLFCLSVFALVGLQLFMGILKQKCVYSTSFTENKEACYKNKTC------RDYINNPSNQYFLPGKKDALICGNSSD----AGQCPEGYTCLKAGN-NPDYGYTSFDSFGWAFLSLFRLMTQDFWENLYQQTLRAAGKTYMIFFVLVIFLGSFYLVNLILAVVAMAY 409 HSP 3 Score: 87.0409 bits (214), Expect = 2.167e-16 Identity = 46/136 (33.82%), Postives = 80/136 (58.82%), Query Frame = 2
Query: 3644 FTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF-FKCVDSNNVRLHESLVQNKSDCYE 4048
+T+ LV++ + F +G + +D W WLDF ++++A ++ F D G+ A+R R LR L+ +S G+K +V ALIQ++ + +V+++ L +F+++G+QLF G KCV S S +NK CY+
Sbjct: 166 YTLEALVKITARGFCVGRFTFLRDPWNWLDFIVIVMAYVTEF--VDLGSVSALRAFRVLRALKTISVVPGLKTIVGALIQSVKKLSDVMILTLFCLSVFALVGLQLFMGILKQKCVYST------SFTENKEACYK 293 HSP 4 Score: 56.9954 bits (136), Expect = 2.904e-7 Identity = 73/318 (22.96%), Postives = 138/318 (43.40%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM--------TKNSES---ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR I+ HS F F++ I+++ A+ T+ S E + +FT I+ E ++K +A G Y FT L W WLDF+++ ++ + V +A L + +LR+LR R L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ + G +C+ + + P++ +N K+ C + +R N N FDN G L ++ T W +Y + N + +YF++ I G+F+ +N+ + ++
Sbjct: 1156 LRQTCYNIVEHSWFETFIVFMILLSSGALAFEDIYLDTRKSVKTILEFADKVFTYIFILEMLLKWVAYGFAKY-FTNL---WCWLDFVIVDVSLISLVANALNYSELSAIKSLRTLRAFRPLRALSRFDGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIVGVNFFAGK-FSECINITTEQRL----------------------------PVSEVYN-------KTDCERMGKGVRWRNVIIN-----FDNVGNGYLALLQVATFKGWMPIMYAAVDSRNPEEQPEYEVNLYMYLYFVIFIILGAFFTLNLFIGVI 1428
BLAST of Sodium channel protein vs. Ensembl Zebrafish
Match: scn1laa (sodium channel, voltage-gated, type I-like, alpha [Source:ZFIN;Acc:ZDB-GENE-040426-751]) HSP 1 Score: 860.907 bits (2223), Expect = 0.000e+0 Identity = 549/1168 (47.00%), Postives = 741/1168 (63.44%), Query Frame = 2
Query: 2156 RISALSVFECENKLRK-RDIYLQR-------FNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNH--IGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMN-KNETE-----PSRNVHDP---------QLNQVAFNLWLENYEPKFDADPDINEA--------------------EVIFWNQPYDCLPDSIRCFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD-SNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKK--------KCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
R ALSV N + +I+ QR F + + W+C P + +K++V IV+DPF +LFITICIV+NTVFM+++ Y +++ N+ F+ IFT E K+IA P YF E WN+FD +IV LS++EL + +S + LR FRLLR+FKLAKSW T+N+L IIG +LGA+ NL +VLAIIVFIFAVVGMQ+FG+ Y + + +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +E D N LQ A+ R+ + ++K L S + + KK K + + DS I + + + ++TE PS V P LN F+ + E + D+ + E++ + Y P+ CF VR CC E I K WW +R + + IV+H +FETFI+ MI+ SS L ED +L TRK +K ILEF D FT IF++EM+LKW A G KYF + WCWLDF IV V+LIS + A K++RTLRA RPLRA+SR+DGM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ F GKF +C++ + RL S V NK+DC + K V W N INFDNV NGYL+L Q+ATFKGW IM AVD QP+Y N + Y+YFV+FII GAFFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRPK+ I + ++++ Q FDI IM I LN + M LE+ Q E++ AL +N +F+++F ECV+K+IALR Y+F + WNVFDFI+VI SI + + + VSPTL RVIR+ RIGRVLRL++SA+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V + GID FNFETF +M+ LFQI+TSGGW+ +L ++N+ +C K CG+ + + + VSY++I FL+++NMYIAVILENFS AT++ L++DD+D FYE WE++DP AT FME LS+F + PL IP+PN+ +L +++P+ G I C+D++ TK LGE
Sbjct: 674 RERALSVASGSNNPAECSEIFKQRCEEWWFEFAENYLIWDCSPTWLRIKEVVHMIVMDPFADLFITICIVINTVFMAMECYPMDGPYSSMLSTANVVFIGIFTAEMIFKVIALDPYSYFLEGWNIFDSIIVTLSLMELCLANVLSG----IGFLRPFRLLRVFKLAKSWPTLNMLIKIIGNSLGALSNLTLVLAIIVFIFAVVGMQLFGKSYGECVCKIS---SDCMLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQPLCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAVSGEE--DENNLQIAVRRIRKGFTFIKTCLQSFFRSGCLRKKKQKSADGNMIVNCKERKDSTNHTTIELVKSTGGTDLDSDTENFISNPSLTVSVPIAAEESDFECLNTEDFSSLSSDLEEDKEKLSDVASSFSEGSTVDLRPLGKDGEDALELVLEEEAY---PEP--CFTAGCVRRFRCCQVDEEKGIWKKWWLLRQTCYNIVEHSWFETFIVFMILLSSGALAFEDIYLDTRKSVKTILEFADKVFTYIFILEMLLKWVAYGFAKYFTNLWCWLDFVIVDVSLISLVANALNYSELSAIKSLRTLRAFRPLRALSRFDGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIVGVNFFAGKFSECINITTEQRLPVSEVYNKTDCERMGK--GVRWRNVIINFDNVGNGYLALLQVATFKGWMPIMYAAVDSRNPEEQPEYEVNLYMYLYFVIFIILGAFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPKNKIQGLIFDLVTKQAFDIFIMVLIWLNMVTMMLETEDQSIEMTRALYWINVIFIVLFTTECVLKMIALRHYFFTVGWNVFDFIVVILSIVGMSLTEVIKKYFVSPTLFRVIRLARIGRVLRLIRSAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKTAGIDDMFNFETFPNSMLCLFQITTSGGWDGLLAPMLNSKPPDCDPTKNFTGSSVVGDCGNPSIGIFFFVSYIIICFLIVVNMYIAVILENFSVATEESAEPLSEDDFDRFYEVWEKFDPKATQFMEYGKLSDFADSLDPPLRIPKPNRFQLMSMDLPMVTGDRIHCLDILFAFTKRVLGE 1824 HSP 2 Score: 300.827 bits (769), Expect = 1.252e-81 Identity = 172/369 (46.61%), Postives = 243/369 (65.85%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAM--TKNSE-SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPEN-----WQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP G+ K LEDLD FY++ TF+V++ K I+RF++T A ++FS FN LR +I+IL H LF+F ++ TI+ NC M T+ + +++VE +FT IYT E ++K ARG V +FT+LRD WNWLDFIVI +AY+ +V+ LG +SALR+ RVLRALKT++ +PG+KT+V +L+ ++ KL D ++LT+F LS+FAL+GLQ++ G L KCV + Y+N + DY NNP N + C N + C E Y C++ N NP++GYTSFD+FG + L FRLMTQD+WENLYQ +R G +MI+F++VIF GSFYL+N+IL++VAM+Y
Sbjct: 56 LEAGKTLPFIYGEV--PKGLVSTPLEDLDQFYSNHTTFIVVSKKKEIYRFNATPALYIFSSFNLLRRISIQILVHFLFNFIIICTILANCAFMVYTQTPDWAKSVEYVFTGIYTLEALVKITARGFCVGRFTFLRDPWNWLDFIVIVMAYVTEFVD--LGSVSALRAFRVLRALKTISVVPGLKTIVGALIQSVKKLSDVMILTLFCLSVFALVGLQLFMGILKQKCVYSTSFTENKEACYKNKTC------RDYINNPSNQYFLPGKKDALICGNSSD----AGQCPEGYTCLKAGN-NPDYGYTSFDSFGWAFLSLFRLMTQDFWENLYQQTLRAAGKTYMIFFVLVIFLGSFYLVNLILAVVAMAY 409 HSP 3 Score: 87.0409 bits (214), Expect = 2.167e-16 Identity = 46/136 (33.82%), Postives = 80/136 (58.82%), Query Frame = 2
Query: 3644 FTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF-FKCVDSNNVRLHESLVQNKSDCYE 4048
+T+ LV++ + F +G + +D W WLDF ++++A ++ F D G+ A+R R LR L+ +S G+K +V ALIQ++ + +V+++ L +F+++G+QLF G KCV S S +NK CY+
Sbjct: 166 YTLEALVKITARGFCVGRFTFLRDPWNWLDFIVIVMAYVTEF--VDLGSVSALRAFRVLRALKTISVVPGLKTIVGALIQSVKKLSDVMILTLFCLSVFALVGLQLFMGILKQKCVYST------SFTENKEACYK 293 HSP 4 Score: 56.9954 bits (136), Expect = 2.904e-7 Identity = 73/318 (22.96%), Postives = 138/318 (43.40%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM--------TKNSES---ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR I+ HS F F++ I+++ A+ T+ S E + +FT I+ E ++K +A G Y FT L W WLDF+++ ++ + V +A L + +LR+LR R L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ + G +C+ + + P++ +N K+ C + +R N N FDN G L ++ T W +Y + N + +YF++ I G+F+ +N+ + ++
Sbjct: 1156 LRQTCYNIVEHSWFETFIVFMILLSSGALAFEDIYLDTRKSVKTILEFADKVFTYIFILEMLLKWVAYGFAKY-FTNL---WCWLDFVIVDVSLISLVANALNYSELSAIKSLRTLRAFRPLRALSRFDGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIVGVNFFAGK-FSECINITTEQRL----------------------------PVSEVYN-------KTDCERMGKGVRWRNVIIN-----FDNVGNGYLALLQVATFKGWMPIMYAAVDSRNPEEQPEYEVNLYMYLYFVIFIILGAFFTLNLFIGVI 1428
BLAST of Sodium channel protein vs. Ensembl Zebrafish
Match: scn12aa (sodium channel, voltage gated, type XII, alpha a [Source:ZFIN;Acc:ZDB-GENE-060908-2]) HSP 1 Score: 858.21 bits (2216), Expect = 0.000e+0 Identity = 539/1158 (46.55%), Postives = 752/1158 (64.94%), Query Frame = 2
Query: 2141 PEMKKRISALSVF-----ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWV--KVK-LYS---TYNHIGKKLMKIIRRHRQI--------KYPFPW--DSNESNPINIQAAIIKMNKNETE-------------PSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCC---SESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
P K+ +S++S F E E +K F + + W C PA+ +K+ + +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+ A P YF + WN+FD +IV LS++EL +S +G LS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + + + +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G CI F+ M++GNLV+LNLFLALLLSSFSS++L DE ++N LQ A+ R++ W+ +K L+S T + K ++ H + KY FP DS +NP I +++ E ++ ++ ++ +P D + A+ P +C P+ F ++ C CC + + + WW +R + ++IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D F+ IF++EM LKW A G KYF + WCWLDF IV V+LIS D GA K++RTLRALRPLRA+SR++GM++VVNALI AIPSI NVLLVCL+FWLIFSI+G+ LF GKF KCV+ + S+V NK++C + T+ W ++NFDNV GYLSL Q+ATFKGW +IM AVD V QP N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QK+ IGG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + F+++++ Q FDI IM I+LN I M +E+ +Q + L+++N F++IF EC+IKI ALR Y+F I WN+FDF++VI SI V+ + VSPTL RVIR+ RIGRVLRL+++A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G++ F+ V GID FNFETFG +MI LFQI+TS GWN++LD ++NN+ EC K CG + + + VSY++ISFL+++NMYIA+ILENFS AT++ L++DD+++FYE WE++D AT F+E LS F + EPL I +PNKI+L +++P+ G I C+D++ TK LGE
Sbjct: 645 PRRKRGLSSVSFFSDAMDELEEARQKCPPCWYEFAQKYLIWTCSPAWLRVKEGLKVMVMDPFLDLAITICIVLNTLFMALEHYPMTDEFNSMLSIGNLVFTGIFTAEMVLKIFALDPYYYFQQGWNIFDGIIVCLSLMEL----GLSNVEG-LSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYQDCVCKISY---DCTLPRWHMKDFFHSFLIVFRVLCGEWIETMWDCMEVAGQPLCILVFMLVMVIGNLVVLNLFLALLLSSFSSDNLSA-PDEDGEMNNLQIAIARIHCGFSWLLNGIKDLFSGNLTRHRRKSKEAQVTLNHTECNGGLGSLEKYVFPETEDSYMTNPNLTICVPIAPGESDVEFLEEEEEEEDTESSEDEEEKQEVISLSEGSTVDLRKPGEDEEEYSEMADDGM--DPEECFPE----FCMQRCKCCNINTTQGLGQMWWRLRKTCYQIVEHSWFETFIIFMILLSSGALAFEDIYIEQRKVVKVVLEYADKVFSYIFVLEMFLKWIAYGFKKYFTNYWCWLDFLIVDVSLISLVANSLGYSDFGAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGKCVNRTGFIHNASIVNNKTECLSMNDTQ-FYWTKVKVNFDNVGLGYLSLLQVATFKGWMEIMYAAVDSRGVEDQPIKEINLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKRMIGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPMNILQGFFFDLVSKQAFDITIMMLIILNMITMMVETDEQSARMETILNNINLAFIVIFTTECLIKIFALRCYFFTISWNIFDFVVVILSIVGIVLADIIEKYFVSPTLFRVIRLARIGRVLRLIRAAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMANFAYVKKQGGIDDMFNFETFGNSMICLFQITTSAGWNNLLDPILNNSPEECSSSFANTGTNTKGNCGHPSVGITFFVSYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFEMFYEVWEKFDVEATHFIEYAKLSNFADSLSEPLRIAKPNKIKLIHMDLPMVNGDRIHCLDILFAFTKRVLGE 1785 HSP 2 Score: 300.827 bits (769), Expect = 1.089e-81 Identity = 177/377 (46.95%), Postives = 248/377 (65.78%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFY-NDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGA---ETYENDSILLWNIKM-----DYYNNPENW----QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LE GKVLP G+ G LED+DPFY +++TF+V+N K IFRFS+T A ++FSPF+ +R +IRIL HSLFS F++ TI+ NC M + ++ +E FT IYT E +IK LARG FT+LRD WNWLDF VI +AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCV+ SIG TY + + N K D+ N +N+ + ++S+ C + ++C++T + NPN+GYTSFD FG + L FRLMTQDYWE+LY +R+ G +MI+F++VIF GSFYL+N+IL++VAM+Y
Sbjct: 55 LEVGKVLPRIFGEI--PAGLVGVPLEDIDPFYFRNQKTFIVLNKGKAIFRFSATSALYIFSPFHCIRRISIRILVHSLFSLFIMCTILTNCCFMAMSDPPLWTKYLEYTFTGIYTFESLIKILARGFCTECFTFLRDPWNWLDFSVIVMAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGILRQKCVR-----SIGHCVNSTYPGNGSFVCNNKTWLSVKDFLTNEDNFYKIEGSKDALICGYSSD---AGKCPDGFDCLKT-SRNPNYGYTSFDTFGWAFLGLFRLMTQDYWESLYHQTLRSAGKTYMIFFVLVIFLGSFYLVNLILAVVAMAY 418 HSP 3 Score: 90.1225 bits (222), Expect = 2.864e-17 Identity = 55/174 (31.61%), Postives = 96/174 (55.17%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTK----YFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVDS 3994
IR + +I+ H F FI+ I+ + + + D L T+ +++ FT I+ E ++K A G + +D W WLDF+++++A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV S
Sbjct: 118 IRRISIRILVHSLFSLFIMCTILTNCCFMAMSDPPLWTK--------YLEYTFTGIYTFESLIKILARGFCTECFTFLRDPWNWLDFSVIVMAYVTEF--VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGILRQKCVRS 281 HSP 4 Score: 63.929 bits (154), Expect = 2.277e-9 Identity = 76/322 (23.60%), Postives = 143/322 (44.41%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAMTKNS---ESENV--------ETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSAL-----GKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYT----SFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNG---------DVHM-IYFMMVIFFGSFYLINVILSIV 1474
LR +I+ HS F F++ I+++ A+ E V + +F+ I+ E +K +A G Y Y W WLDF+++ ++ + V ++L G + +LR+LR LR L+ ++ GM+ VV++L+ AI + + L++ + IF+++G+ ++ G KCV N F HN S N + EC+ ++ F +T +FDN G+ L ++ T W + + + G +++M +YF++ I FGSF+ +N+ + ++
Sbjct: 1117 LRKTCYQIVEHSWFETFIIFMILLSSGALAFEDIYIEQRKVVKVVLEYADKVFSYIFVLEMFLKWIAYGFKKYFTNY----WCWLDFLIVDVSLISLVANSLGYSDFGAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK-FGKCV-----------------------------------NRTGFIHN-------ASIVNNKTECLSMNDT--QFYWTKVKVNFDNVGLGYLSLLQVATFKGWMEIMYAAVDSRGVEDQPIKEINLYMYLYFVIFIIFGSFFTLNLFIGVI 1389
BLAST of Sodium channel protein vs. Ensembl Xenopus
Match: gmppa (GDP-mannose pyrophosphorylase A [Source:Xenbase;Acc:XB-GENE-984239]) HSP 1 Score: 1128.24 bits (2917), Expect = 0.000e+0 Identity = 766/1809 (42.34%), Postives = 1060/1809 (58.60%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFF--VLTTIIINCIAMTKNSESE---NVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMD--YYNNPENW------QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDDKRFSDLASCQVNTSKAQHDSLILNHQLSSN----------------------------------------KINKV---DSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALS---VFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYS-TYNHIGKKLMKIIRR--------------------HRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLN---QVAF------NLWLENYEPKFDADP----------------DIN-EAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL--QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
LEAGK +P GD + + +EDLDPFY +TF+V+N KTIFRFS+ A ++ SPFNPLR AI+IL HS + FF ++ TI+ NC+ MT ++ E VE FT IYT E ++K +ARG + FT+LRD WNWLDF VI +AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCV W + MD Y N N+ +P+ C N + C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R +G +MI+F++VIF GSFYL+N+IL++VAM+Y++ A + E E +E E K E Q+ + + DA D+ + + KS + R + + L K E K+ + D + + DN L S P +R FR D + + F+D V S+ + DSL + SS KI+K DS +E + + S + ++ L + +S ++ V E E RK +F T+ W CCP + +K IV+ IV+DPF++L ITICIVLNT+FM+++ Y + ++ NL F IFT E +KLIA P YF E WN+FD IV LS++EL +D+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y K+ +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G CI F+ M++GNLV+LNLFLALLLSSFS+++L +D+ ++N LQ ++ R+ + I W+K+K + H KK ++ HR + YP + S I +++ K +E S +H+P L +A NL E++ D + DI EAE + QP D H E + K+WW +R + F IV+H +FETFI+ MI+ SS L ED ++ RK ++ ILE+ D FT IF++EM+LKW A G K+F +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GK++ C + + E V+N++ C++L V W N +INFDNV GYL+L Q+ATFKGW DIM AVD +V+ QPKY +N + YIYFV+FIIFG+FFTLNLFIGVIIDNFN QKKKI S ++FMT+ QK+YY+AMKK+ S PQKP+PRP + ++II Q FDIVIM I LN + M +E+ Q + L +N VF++ F ECV+K+ ALR YYF I WN+FDF++VI SI + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFI++I G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N +C K+ CG+ + + + VSY++ISFL+++NMYIA+ILENFS AT++ L++DD++ FYE WE++DP AT F++ L +F + PL IP+PN I L +++P+ G I C+D++ TK LGE
Sbjct: 59 LEAGKGVPFIYGDC--PSRLIAEPIEDLDPFYLHTKTFIVLNRGKTIFRFSAAPALYILSPFNPLRKFAIKILIHS-YPFFMIIMCTILTNCVFMTFSNPPEWSKQVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRRKCVA-------------------WPVDMDQMYLANGTNFFKVHGAIDPL-LCGNGSD----AGQCPEGYTCMKA-GRNPNYGYTSFDTFSWAFLALFRLMTQDFWENLYQLTLRASGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAERKENEFKAMLE---QMKRMQEEAQDAVEDEDGEARMSHTSSEFSKPSSKSAKERRNRRKKRKQKELSEGEEKGDSE--KVYKSESEDGVKRRGFWYPDNRLGRSIPGSPYLSRHNSKSSIFSFRGRFRD--------PVSENEFADDEHSTVEESEGRRDSLFIPGSRSSRILQNLRRNPKRNSTVDCNGVVSLIGGPGSNIPGGRLLPEVKIDKAATDDSPTTEVEVKRKPSGSLRVSMDQLTASFGRKRANSILSVVTQTLVEELEESQRKCPPCWYKFADTYLIWTCCPLWIRIKTIVNMIVMDPFVDLTITICIVLNTLFMAMEHYPMTPHFENVLVVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMEL-GLQDVEG----LSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY---KDCVCKINPECVLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCIIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDD-GEMNNLQISVIRIKKGIAWIKLKAHDFMQKHFKKKDADEVKPLDEMYEKKMNCLANNTGADIHRDMDYPKNGNGTTSG---IGSSVEKYMIDEDHMSF-IHNPNLTVKVPIAVGESDFENLNTEDFSSASDEEGSKDKLDDTSSSEGSTIDIKPEAEEVPVEQPED------------HIVFSVEEGLGKSWWTLRKTCFLIVEHNWFETFIIFMILLSSGALAFEDVYIEQRKTIRTILEYADKVFTYIFILEMLLKWLAYGFVKFFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYYCYNETAEGMFEIEDVENETQCFDLIGNNYTEVRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKVHQQPKYEDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKI--SQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKFQGAIFDIITQQAFDIVIMILICLNMVTMMVETDDQSDYTDNVLYWINVVFIVFFTTECVLKLCALRHYYFTIGWNIFDFVVVILSIVGMFLADLITKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPP-DCDAHKENPGSQVKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIQFSKLPDFADALEHPLRIPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1796
BLAST of Sodium channel protein vs. Ensembl Xenopus
Match: gmppa (GDP-mannose pyrophosphorylase A [Source:Xenbase;Acc:XB-GENE-984239]) HSP 1 Score: 1066.22 bits (2756), Expect = 0.000e+0 Identity = 745/1807 (41.23%), Postives = 1050/1807 (58.11%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFF--VLTTIIINCIAMTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKM-----DYYNNPENW------QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNML-------------IDSCPSLHRTN-RFKVYQQFNVFRKLIEDNRKESSKYSLDDKRFSDLASCQVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRI---------------SNIVLEDLEGLYNNI----------NEPEMKKRISA---------------------LSVF------ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYS-TYNHIGKKLMKIIRR--------------------HRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL--QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
LEAGK +P GD + + +EDLDPFY +TF+V+N KTIFRFS+ A ++ SPFNPLR AI+IL HS + FF ++ TI+ NC+ MT ++ E + + AI K + GL+ + L+ + A + +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCV A + D + L N +Y N N+ +P+ C N + C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R +G +MI+F++VIF GSFYL+N+IL++VAM+Y + A EE E ++ + EQ+ + + DA D+ + + KS + R + + L K E K+ + D + + DN L I P L R N + ++ FR + +N F+D V S+ + DSL + + ++ N +S + R SRI N V+ + G +NI E E+K++ S LSV E E RK +F T+ W CCP + +K IV+ IV+DPF++L ITICIVLNT+FM+++ Y + ++ NL F IFT E +KLIA P YF E WN+FD IV LS++EL +D+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y K+ +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G CI F+ M++GNLV+LNLFLALLLSSFS+++L +D+ ++N LQ ++ R+ + I W+K+K + H KK ++ HR + YP + S I +++ K +E S +H+P L +V + ++E F + D ++ + + L + + H E + K+WW +R + F IV+H +FETFI+ MI+ SS L ED ++ RK ++ ILE+ D FT IF++EM+LKW A G K+F +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GK++ C + + E V+N++ C++L V W N +INFDNV GYL+L Q+ATFKGW DIM AVD +V+ QPKY +N + YIYFV+FIIFG+FFTLNLFIGVIIDNFN QKKKI S ++FMT+ QK+YY+AMKK+ S PQKP+PRP + ++II Q FDIVIM I LN + M +E+ Q + L +N VF++ F ECV+K+ ALR YYF I WN+FDF++VI SI + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFI++I G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N +C K+ CG+ + + + VSY++ISFL+++NMYIA+ILENFS AT++ L++DD++ FYE WE++DP AT F++ L +F + PL IP+PN I L +++P+ G I C+D++ TK LGE
Sbjct: 59 LEAGKGVPFIYGDC--PSRLIAEPIEDLDPFYLHTKTFIVLNRGKTIFRFSAAPALYILSPFNPLRKFAIKILIHS-YPFFMIIMCTILTNCVFMTFSNPPEWSKQVDPAI------TKVVNVGLL----SPLKPS-----------APLQFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLRRKCV---------AWPVDMDQMYLANGSKGFDFDEYIMNDTNFFKVHGAIDPL-LCGNGSD----AGQCPEGYTCMKA-GRNPNYGYTSFDTFSWAFLALFRLMTQDFWENLYQLTLRASGKTYMIFFVLVIFVGSFYLVNLILAVVAMAY---EEQNQATLEEAERKENEFKAMLEQMKRMQEEAQDAVEDEDGEARMSHTSSEFSKPSSKSAKERRNRRKKRKQKELSEGEEKGDSE--KVYKSESEDGVKRRGFWYPDNRLGRRFSIMNQSLLSIPGSPYLSRHNSKSSIFSFRGRFRDPVSEN------------EFADDEHSTVEESEGRRDSLFIPVR-GHDRRNSYNSGYSQGSRSSRILQNLRRNPKRNSTVDCNGVVSLIGGPGSNIPGGRLLPEPTTEVEVKRKPSGSLRVSMDQLTASFGRKRANSILSVVTQTLVEELEESQRKCPPCWYKFADTYLIWTCCPLWIRIKTIVNMIVMDPFVDLTITICIVLNTLFMAMEHYPMTPHFENVLVVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMEL-GLQDVEG----LSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY---KDCVCKINPECVLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCIIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDD-GEMNNLQISVIRIKKGIAWIKLKAHDFMQKHFKKKDADEVKPLDEMYEKKMNCLANNTGADIHRDMDYPKNGNGTTS---GIGSSVEKYMIDEDHMSF-IHNPNLTVKVPIAVGESDFENLNTEDFSSASDEEGSKDVLSRMFHSVLCSTAFGCVSSHIVFSVEEGLGKSWWTLRKTCFLIVEHNWFETFIIFMILLSSGALAFEDVYIEQRKTIRTILEYADKVFTYIFILEMLLKWLAYGFVKFFTNAWCWLDFLIVDVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYYCYNETAEGMFEIEDVENETQCFDLIGNNYTEVRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKVHQQPKYEDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKI--SQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKFQGAIFDIITQQAFDIVIMILICLNMVTMMVETDDQSDYTDNVLYWINVVFIVFFTTECVLKLCALRHYYFTIGWNIFDFVVVILSIVGMFLADLITKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPP-DCDAHKENPGSQVKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIQFSKLPDFADALEHPLRIPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1790
BLAST of Sodium channel protein vs. Ensembl Xenopus
Match: SCN4A (sodium channel, voltage gated, type IV alpha subunit [Source:NCBI gene;Acc:100492371]) HSP 1 Score: 894.034 bits (2309), Expect = 0.000e+0 Identity = 546/1138 (47.98%), Postives = 753/1138 (66.17%), Query Frame = 2
Query: 2132 INEPEMKKRI----SALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDA-----DPDIN-EAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCIT---KAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALIS----YFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
+ EP ++KR S LS E +K + +F TF W+CC + K+ V F+V+DPF++L ITICIVLNT+FM+++ Y + ++N NL F IF E K+IA P YF WN+FD +IV LS+VEL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + + ++PRW+ DF HSF+IVFR+LCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +D+ ++N LQ A+ R+ R I +VK +S H +K K + E+ P + I + E EP + P ++ V + Y +FD +P + E + +D +PD+ VR C C T K WW IR + F+IV+H +FETFI+ MI+ SS L ED ++ R+ +K ILE+ D FT IF+ EM+LKW A G YF +AWCWLDF IV V+LIS + + G K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GK+++C+++ L E +V N+SDC L +E V WVN ++NFDNV GYLSL Q+ATFKGW DIM AVD + QP+Y N + Y+YFV+FIIFGAFFTLNLFIGVIIDNFN QKKKI S ++FMT+ QK+YY+AMKK+ S PQKP+PRP + I + Y+ + Q FDIVIM I LN + M +E+ Q +E +D L +N VF++IF ECV+K+ ALR+Y+F I WN+FDF++VI SI V+ + VSPTL RVIR+ RIGRVLRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIY+I G+S F+ V GID FNFETFG ++I LFQI+TS GW+ +L+ ++N+ +C CG+ + +++ SY++ISFL+++NMYIA+ILENF+ AT++ L +DD+++FYE WE++DP AT F+E +S+FV + +PL IP+PNKI+L L++P+ G I C+D++ LTK LG+
Sbjct: 500 LEEPTLQKRAESTHSVLSEVALEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI---ASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDD-GEMNNLQIAIGRITRGIDFVKKTFFSIL-HYKRKAKAEQEEEDPNKENIALNHVETEP---EPKIELEPRTEPEPKK--EPPLIDGVTKKRY---YMDEFDHANFFNNPHMRIEVPIASEESDFD-IPDTDEFNCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKI--SKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIVIMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVVVILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDCDPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFEMFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFALTKEVLGD 1616 HSP 2 Score: 315.849 bits (808), Expect = 2.276e-86 Identity = 179/375 (47.73%), Postives = 254/375 (67.73%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHS--LFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK---------VFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P + G +E+LD FY++++TF+V+N K IFRF++TKA +L PFNP+R AI++L HS L + F++ TI+ NC+ MT + S+NVE FT IYT E +IK LARG + FT+LRD WNWLDF VI +AY +V+ LG +SALR+ RVLRALKT+T IPG+KT+V +L+ ++ KL D ++LT+F L++FALIGLQ++ G L HKCV+ ++DNS++ + Y ND+++ + Y+ + N C N + C E Y C++T NPN+GYTS+D+F + L FRLMTQD+WENL+QL +R G +M++F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 53 LEAGKNLPLIYGD--PPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSYPLINLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVD--LGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLY-NDTMINSTLTNFYF---LDGANDALLCGNSSD----AGKCPESYTCMKT-GRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAY 414 HSP 3 Score: 83.1889 bits (204), Expect = 4.226e-15 Identity = 55/174 (31.61%), Postives = 93/174 (53.45%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKF--FETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF-FKCV 3988
IR A K++ H + FI+ I+ + + +T+ D P K + + FT I+ E ++K A G + +D W WLDF ++++A + F D G A+RT R LR L+ ++ G+K +V ALIQ++ + +V+++ L +F++IG+QLF G KCV
Sbjct: 115 IRRGAIKVLIHSYPLINLFIMFTILTNCVFMTMSDP-PPWSKNV-------EYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEF--VDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCV 278 HSP 4 Score: 60.4622 bits (145), Expect = 3.455e-8 Identity = 72/318 (22.64%), Postives = 145/318 (45.60%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAMT-----------KNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVN-----SALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNG---------DVHM-IYFMMVIFFGSFYLINVILSIV 1474
+R +I+ H+ F F++ I+++ A+ + E + +FT I+ E ++K A G +Y FT +AW WLDF+++ ++ + S LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G ++C+ ++ +E +E D + NN E +R N N FDN G+ L ++ T W ++ + + +++M +YF++ I FG+F+ +N+ + ++
Sbjct: 947 IRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIY-FT---NAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK-YYRCI-----NTTTSELFEIDVV----------------------------NNRSDCLALNNSEQVRWVNVKVN-----FDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVI 1221
BLAST of Sodium channel protein vs. Ensembl Xenopus
Match: SCN4A (sodium channel, voltage gated, type IV alpha subunit [Source:NCBI gene;Acc:100492371]) HSP 1 Score: 877.856 bits (2267), Expect = 0.000e+0 Identity = 559/1235 (45.26%), Postives = 777/1235 (62.91%), Query Frame = 2
Query: 1904 EDNRKESSKYSLDDKRFSDLASCQVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMK-------KRISALSVF-ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVH-DPQLNQVAFNLWLENYEPKFDA---DPDINEAEVIFWNQPY----------------DCLPDSIRCFL---VRHCNCCSESCIT---KAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALIS----YFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
E N + +K F +L Q+ + + + + LS + +D + + +ED+ YN + P + + L F E E +K + +F TF W+CC + K+ V F+V+DPF++L ITICIVLNT+FM+++ Y + ++N NL F IF E K+IA P YF WN+FD +IV LS+VEL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + + ++PRW+ DF HSF+IVFR+LCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +D+ ++N LQ A+ R+ R I +VK +S ++ K+ K + D N+ N I +N ETEP + +P+ EPK + D ++ + Y D P+ CF VR C C T K WW IR + F+IV+H +FETFI+ MI+ SS L ED ++ R+ +K ILE+ D FT IF+ EM+LKW A G YF +AWCWLDF IV V+LIS + + G K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GK+++C+++ L E +V N+SDC L +E V WVN ++NFDNV GYLSL Q+ATFKGW DIM AVD N QP+Y N + Y+YFV+FIIFGAFFTLNLFIGVIIDNFN QKKKI S ++FMT+ QK+YY+AMKK+ S PQKP+PRP + I + Y+ + Q FDIVIM I LN + M +E+ Q +E +D L +N VF++IF ECV+K+ ALR+Y+F I WN+FDF++VI SI V+ + VSPTL RVIR+ RIGRVLRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIY+I G+S F+ V GID FNFETFG ++I LFQI+TS GW+ +L+ ++N+ +C CG+ + +++ SY++ISFL+++NMYIA+ILENF+ AT++ L +DD+++FYE WE++DP AT F+E +S+FV + +PL IP+PNKI+L L++P+ G I C+D++ LTK LG+
Sbjct: 424 EQNEATLQEEIEKEKEFQELL-LQLKKHQEEQKKALSENTLSGDGTENMDCNGRTVPKVIIERAPSMEDVSIKYNLTSLPRIHNFSNTVPPGCTNLCTFTELEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI---ASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDD-GEMNNLQIAIGRITRGIDFVKKTFFSILHY--KRKAKAEQEEE--------DPNKEN--------IALNHVETEPEPKIELEPRTEP----------EPKKEPPLIDGKYDDGSSVCSTADYKPPEPEEEEEAFEHKEDEGPE--ECFTADCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVD--SRNDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKI--SKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIVIMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVVVILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDCDPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFEMFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFALTKEVLGD 1614 HSP 2 Score: 313.923 bits (803), Expect = 9.820e-86 Identity = 181/379 (47.76%), Postives = 254/379 (67.02%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHS--LFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSI-----LLWNIKMD---YYNNPENW-----QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P + G +E+LD FY++++TF+V+N K IFRF++TKA +L PFNP+R AI++L HS L + F++ TI+ NC+ MT + S+NVE FT IYT E +IK LARG + FT+LRD WNWLDF VI +AY +V+ LG +SALR+ RVLRALKT+T IPG+KT+V +L+ ++ KL D ++LT+F L++FALIGLQ++ G L HKCV+ NS++ +T N ++ + N +D Y N+ N+ N C N + C E Y C++T NPN+GYTS+D+F + L FRLMTQD+WENL+QL +R G +M++F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 53 LEAGKNLPLIYGD--PPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSYPLINLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVD--LGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRCTLNSTLYNDTMINSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSD----AGKCPESYTCMKT-GRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAY 422 HSP 3 Score: 86.2705 bits (212), Expect = 6.153e-16 Identity = 61/216 (28.24%), Postives = 110/216 (50.93%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKF--FETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF-FKCVDSN------NVRLHESLVQNKSDCYELQKTENVSWVNSQINF 4096
IR A K++ H + FI+ I+ + + +T+ D P K + + FT I+ E ++K A G + +D W WLDF ++++A + F D G A+RT R LR L+ ++ G+K +V ALIQ++ + +V+++ L +F++IG+QLF G KCV N + S + N ++ ++ + +++N + NF
Sbjct: 115 IRRGAIKVLIHSYPLINLFIMFTILTNCVFMTMSDP-PPWSKNV-------EYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEF--VDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRCTLNSTLYNDTMINSTLSNSTNVFDNDTIDWGAYLNDEANF 320 HSP 4 Score: 63.5438 bits (153), Expect = 4.549e-9 Identity = 73/316 (23.10%), Postives = 145/316 (45.89%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAMT-----------KNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVN-----SALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGD-------VHM-IYFMMVIFFGSFYLINVILSIV 1474
+R +I+ H+ F F++ I+++ A+ + E + +FT I+ E ++K A G +Y FT +AW WLDF+++ ++ + S LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G ++C+ ++ +E +E D + NN E +R N N FDN G+ L ++ T W ++ + + D ++M +YF++ I FG+F+ +N+ + ++
Sbjct: 947 IRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIY-FT---NAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK-YYRCI-----NTTTSELFEIDVV----------------------------NNRSDCLALNNSEQVRWVNVKVN-----FDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVI 1219
BLAST of Sodium channel protein vs. Ensembl Xenopus
Match: SCN4A (sodium channel, voltage gated, type IV alpha subunit [Source:NCBI gene;Acc:100492371]) HSP 1 Score: 877.085 bits (2265), Expect = 0.000e+0 Identity = 566/1274 (44.43%), Postives = 790/1274 (62.01%), Query Frame = 2
Query: 1889 FRKLIEDNRKESSKYSLDDKRFSDLASCQVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMK-------KRISALSVF-ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVA--------------FN-------LWLENYEPKFDADPDINE-------AEVIFWNQPYD-----CLPDSIR---------------------CFL---VRHCNCCSESCIT---KAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALIS----YFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
F++L+ +K + + +R S +AS V A+ + + LS + +D + + +ED+ YN + P + + L F E E +K + +F TF W+CC + K+ V F+V+DPF++L ITICIVLNT+FM+++ Y + ++N NL F IF E K+IA P YF WN+FD +IV LS+VEL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + + ++PRW+ DF HSF+IVFR+LCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +D+ ++N LQ A+ R+ R I +VK +S ++ K+ K + D N+ N I ++ TEP P ++ V FN + + + E FD PD +E E I ++ YD C + CF VR C C T K WW IR + F+IV+H +FETFI+ MI+ SS L ED ++ R+ +K ILE+ D FT IF+ EM+LKW A G YF +AWCWLDF IV V+LIS + + G K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GK+++C+++ L E +V N+SDC L +E V WVN ++NFDNV GYLSL Q+ATFKGW DIM AVD + QP+Y N + Y+YFV+FIIFGAFFTLNLFIGVIIDNFN QKKKI S ++FMT+ QK+YY+AMKK+ S PQKP+PRP + I + Y+ + Q FDIVIM I LN + M +E+ Q +E +D L +N VF++IF ECV+K+ ALR+Y+F I WN+FDF++VI SI V+ + VSPTL RVIR+ RIGRVLRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIY+I G+S F+ V GID FNFETFG ++I LFQI+TS GW+ +L+ ++N+ +C CG+ + +++ SY++ISFL+++NMYIA+ILENF+ AT++ L +DD+++FYE WE++DP AT F+E +S+FV + +PL IP+PNKI+L L++P+ G I C+D++ LTK LG+
Sbjct: 453 FQELLLQLKKHQEEQA---RRKSSVAS--VGLLNAEKKKALSENTLSGDGTENMDCNGRTVPKVIIERAPSMEDVSIKYNLTSLPRIHNFSNTVPPGCTNLCTFTELEEAHQKCPPWWYKFANTFLIWDCCDLWVKFKEFVRFVVMDPFVDLGITICIVLNTLFMAMEHYPMTEQFEGVLNVGNLVFTGIFAAEMFFKIIALDPYYYFQVGWNIFDSIIVTLSLVELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI---ASDCELPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLVVFMMVMVIGNLVVLNLFLALLLSSFSADNLSASDDD-GEMNNLQIAIGRITRGIDFVKKTFFSILHY--KRKAKAEQEEE--------DPNKENIIELEP--------RTEPEPKKEPPLIDGVTKKRYYMDEFDHANFFNNPHMRIEVPIASEESDFDI-PDTDEFSTQSEVEEGILHHKKYDDGSSVCSTADYKPPEPEEEEEAFEHKEDEGPEECFTADCVRRCPCLYVDITTEKGKIWWNIRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIYFTNAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYYRCINTTTSELFEIDVVNNRSDCLALNNSEQVRWVNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVIIDNFNQQKKKI--SKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPTNKIQGLVYDFVTKQLFDIVIMILICLNMVTMMIETDDQSQEKTDILFKINLVFIVIFTAECVLKMFALRYYFFTIGWNIFDFVVVILSIVGIVLSDLIAEYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDIFNFETFGNSIICLFQITTSAGWDGLLNPILNSGPPDCDPHLENFGTTVTGDCGNPAIGIVFFCSYIIISFLIVVNMYIAIILENFNVATEESGEPLCEDDFEMFYETWEKFDPDATQFVEYSRMSDFVDTLQDPLRIPKPNKIKLITLDLPMVTGDKIHCLDILFALTKEVLGD 1691 HSP 2 Score: 314.694 bits (805), Expect = 7.820e-86 Identity = 182/389 (46.79%), Postives = 258/389 (66.32%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHS--LFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK---------VFDNSSIGAETYENDSIL---LWNIKMDYYNNPENWQNPITFCHNF-----------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P + G +E+LD FY++++TF+V+N K IFRF++TKA +L PFNP+R AI++L HS L + F++ TI+ NC+ MT + S+NVE FT IYT E +IK LARG + FT+LRD WNWLDF VI +AY +V+ LG +SALR+ RVLRALKT+T IPG+KT+V +L+ ++ KL D ++LT+F L++FALIGLQ++ G L HKCV+ ++DNS++ + Y ND+++ L N + N+ +W + NF N+ C E Y C++T NPN+GYTS+D+F + L FRLMTQD+WENL+QL +R G +M++F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 53 LEAGKNLPLIYGD--PPPELIGIPIEELDSFYSNQKTFIVLNKGKAIFRFTATKALYLLDPFNPIRRGAIKVLIHSYPLINLFIMFTILTNCVFMTMSDPPPWSKNVEYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEFVD--LGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCVRWPPPDDYFMLYDNSTLNSTLY-NDTMINSTLSNSTNVFDNDTIDWGAYLNDEANFYFLDGANDALLCGNSSDAGKCPESYTCMKT-GRNPNYGYTSYDSFNWAFLSLFRLMTQDFWENLFQLTLRAAGKTYMVFFVVVIFLGSFYLINLILAVVAMAY 435 HSP 3 Score: 83.5741 bits (205), Expect = 3.489e-15 Identity = 55/174 (31.61%), Postives = 93/174 (53.45%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKF--FETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF-FKCV 3988
IR A K++ H + FI+ I+ + + +T+ D P K + + FT I+ E ++K A G + +D W WLDF ++++A + F D G A+RT R LR L+ ++ G+K +V ALIQ++ + +V+++ L +F++IG+QLF G KCV
Sbjct: 115 IRRGAIKVLIHSYPLINLFIMFTILTNCVFMTMSDP-PPWSKNV-------EYTFTGIYTFESLIKILARGFCIDSFTFLRDPWNWLDFCVIVMAYTTEF--VDLGNVSALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLADVMILTLFCLAVFALIGLQLFMGNLRHKCV 278 HSP 4 Score: 60.4622 bits (145), Expect = 3.363e-8 Identity = 72/318 (22.64%), Postives = 145/318 (45.60%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAMT-----------KNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVN-----SALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNG---------DVHM-IYFMMVIFFGSFYLINVILSIV 1474
+R +I+ H+ F F++ I+++ A+ + E + +FT I+ E ++K A G +Y FT +AW WLDF+++ ++ + S LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G ++C+ ++ +E +E D + NN E +R N N FDN G+ L ++ T W ++ + + +++M +YF++ I FG+F+ +N+ + ++
Sbjct: 1022 IRKTCFQIVEHNWFETFIIFMILLSSGALAFEDIYIEQRQVIKTILEYADKVFTYIFVFEMLLKWTAYGFKIY-FT---NAWCWLDFLIVDVSLISLTANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK-YYRCI-----NTTTSELFEIDVV----------------------------NNRSDCLALNNSEQVRWVNVKVN-----FDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNIEDQPQYEINLYMYLYFVIFIIFGAFFTLNLFIGVI 1296
BLAST of Sodium channel protein vs. Ensembl Mouse
Match: Scn1a (sodium channel, voltage-gated, type I, alpha [Source:MGI Symbol;Acc:MGI:98246]) HSP 1 Score: 882.478 bits (2279), Expect = 0.000e+0 Identity = 577/1350 (42.74%), Postives = 823/1350 (60.96%), Query Frame = 2
Query: 1691 QNLPKSHVSLYLPRTKSLQEISKL-----NVDQKSDIAESKHKESNDN-MLIDS--CPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDD--KRFSDLASCQVNTSKAQH--DSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALS------VFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWD---------------------------SNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAE-------------VIFWNQPYDCLP--------DSIRCFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDK--------KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
Q+L SL+ PR S + +V ++D A+ +H DN DS P H R Q + +++ +S D S + V TS +I++ + + N ++ +R S ++ ++ LE +P ++R +++ V E E +K +F+ F W+C P + +K IV+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS+VEL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y K+ + K+PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +D++ ++N LQ A+ R+++ I +VK K+ Y I + +K + +IK P D + ++ I +++ K +E++ +++P L V + ++E F ++ D+ E++ + P + P + CF V+ CC E K WW +R + F+IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ CV++ + E S V N SDC +L ++ E W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY + + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q ++ L +N VF+++F ECV+K+I+LR YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C +K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT FME + LS+F A + PL +PQPNK++L +++P+ G I C+D++ TK LGE
Sbjct: 554 QSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDD--NGTTTETEMRKRRSSSFHVSMDFLE-------DPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY---KDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDN-EMNNLQIAVDRMHKGIAYVKRKI---YEFIQQSFVKKQKILDEIK---PLDDLNNRKDNCISNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVIEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1878 HSP 2 Score: 319.701 bits (818), Expect = 1.921e-87 Identity = 187/383 (48.83%), Postives = 252/383 (65.80%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK-VFDNSSIGAETYENDSILLWNIKMDYYNNPEN-------WQNPI--TFCHNF---------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ + LEDLDP+Y +K+TF+V+N K IFRFS+T A ++ +PFNPLR AI+IL HSLFS ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK +ARG + FT+LRD WNWLDF VI AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KCV+ N+S+ + E NI MDY N W++ I + H F N+ C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 55 LEAGKNLPFIYGDIPPE--MVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEK------NITMDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 426 HSP 3 Score: 87.8113 bits (216), Expect = 1.385e-16 Identity = 57/187 (30.48%), Postives = 97/187 (51.87%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVD--SNNVRLHESLVQ 4027
+R A KI+ H F I+ I+ + + +T+ + D + ++ FT I+ E ++K A G + +D W WLDFT++ A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV N L E ++
Sbjct: 117 LRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNP--------PDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEF--VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIE 293 HSP 4 Score: 73.559 bits (179), Expect = 3.262e-12 Identity = 76/323 (23.53%), Postives = 144/323 (44.58%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-STCNEEYECIRTDNENPNFGY----TSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR RI+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G YQ TY +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H C N T+ ++ + S N +C++ N + +FDN G L ++ T W + +Y + N ++ +YF++ I FGSF+ +N+ + ++
Sbjct: 1207 LRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYG---YQ-TYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-------------------------------------------CVNTTTGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVI 1482
BLAST of Sodium channel protein vs. Ensembl Mouse
Match: Scn1a (sodium channel, voltage-gated, type I, alpha [Source:MGI Symbol;Acc:MGI:98246]) HSP 1 Score: 881.708 bits (2277), Expect = 0.000e+0 Identity = 546/1191 (45.84%), Postives = 767/1191 (64.40%), Query Frame = 2
Query: 2132 INEPEMKKRISAL------SVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWD---------------------------SNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAE-------------VIFWNQPYDCLP--------DSIRCFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDK--------KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
+ +P ++R ++ +V E E +K +F+ F W+C P + +K IV+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS+VEL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y K+ + K+PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +D++ ++N LQ A+ R+++ I +VK K+ Y I + +K + +IK P D + ++ I +++ K +E++ +++P L V + ++E F ++ D+ E++ + P + P + CF V+ CC E K WW +R + F+IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ CV++ + E S V N SDC +L ++ E W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY + + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q ++ L +N VF+++F ECV+K+I+LR YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C +K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT FME + LS+F A + PL +PQPNK++L +++P+ G I C+D++ TK LGE
Sbjct: 676 LEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY---KDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDN-EMNNLQIAVDRMHKGIAYVKRKI---YEFIQQSFVKKQKILDEIK---PLDDLNNRKDNCISNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVIEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1850 HSP 2 Score: 319.701 bits (818), Expect = 1.934e-87 Identity = 187/383 (48.83%), Postives = 252/383 (65.80%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK-VFDNSSIGAETYENDSILLWNIKMDYYNNPEN-------WQNPI--TFCHNF---------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ + LEDLDP+Y +K+TF+V+N K IFRFS+T A ++ +PFNPLR AI+IL HSLFS ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK +ARG + FT+LRD WNWLDF VI AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KCV+ N+S+ + E NI MDY N W++ I + H F N+ C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 55 LEAGKNLPFIYGDIPPE--MVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEK------NITMDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 426 HSP 3 Score: 87.8113 bits (216), Expect = 1.464e-16 Identity = 57/187 (30.48%), Postives = 97/187 (51.87%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVD--SNNVRLHESLVQ 4027
+R A KI+ H F I+ I+ + + +T+ + D + ++ FT I+ E ++K A G + +D W WLDFT++ A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV N L E ++
Sbjct: 117 LRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNP--------PDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEF--VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIE 293 HSP 4 Score: 73.559 bits (179), Expect = 3.067e-12 Identity = 76/323 (23.53%), Postives = 144/323 (44.58%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-STCNEEYECIRTDNENPNFGY----TSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR RI+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G YQ TY +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H C N T+ ++ + S N +C++ N + +FDN G L ++ T W + +Y + N ++ +YF++ I FGSF+ +N+ + ++
Sbjct: 1179 LRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYG---YQ-TYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-------------------------------------------CVNTTTGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVI 1454
BLAST of Sodium channel protein vs. Ensembl Mouse
Match: Scn1a (sodium channel, voltage-gated, type I, alpha [Source:MGI Symbol;Acc:MGI:98246]) HSP 1 Score: 880.937 bits (2275), Expect = 0.000e+0 Identity = 546/1191 (45.84%), Postives = 767/1191 (64.40%), Query Frame = 2
Query: 2132 INEPEMKKRISALS------VFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWD---------------------------SNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAE-------------VIFWNQPYDCLP--------DSIRCFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDK--------KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
+ +P ++R +++ V E E +K +F+ F W+C P + +K IV+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS+VEL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y K+ + K+PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +D++ ++N LQ A+ R+++ I +VK K+ Y I + +K + +IK P D + ++ I +++ K +E++ +++P L V + ++E F ++ D+ E++ + P + P + CF V+ CC E K WW +R + F+IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ CV++ + E S V N SDC +L ++ E W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY + + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q ++ L +N VF+++F ECV+K+I+LR YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C +K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT FME + LS+F A + PL +PQPNK++L +++P+ G I C+D++ TK LGE
Sbjct: 693 LEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY---KDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDN-EMNNLQIAVDRMHKGIAYVKRKI---YEFIQQSFVKKQKILDEIK---PLDDLNNRKDNCISNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVIEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1867 HSP 2 Score: 319.701 bits (818), Expect = 2.229e-87 Identity = 187/383 (48.83%), Postives = 252/383 (65.80%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK-VFDNSSIGAETYENDSILLWNIKMDYYNNPEN-------WQNPI--TFCHNF---------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ + LEDLDP+Y +K+TF+V+N K IFRFS+T A ++ +PFNPLR AI+IL HSLFS ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK +ARG + FT+LRD WNWLDF VI AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KCV+ N+S+ + E NI MDY N W++ I + H F N+ C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 55 LEAGKNLPFIYGDIPPE--MVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEK------NITMDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 426 HSP 3 Score: 87.8113 bits (216), Expect = 1.505e-16 Identity = 57/187 (30.48%), Postives = 97/187 (51.87%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVD--SNNVRLHESLVQ 4027
+R A KI+ H F I+ I+ + + +T+ + D + ++ FT I+ E ++K A G + +D W WLDFT++ A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV N L E ++
Sbjct: 117 LRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNP--------PDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEF--VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIE 293 HSP 4 Score: 73.559 bits (179), Expect = 3.398e-12 Identity = 76/323 (23.53%), Postives = 144/323 (44.58%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-STCNEEYECIRTDNENPNFGY----TSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR RI+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G YQ TY +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H C N T+ ++ + S N +C++ N + +FDN G L ++ T W + +Y + N ++ +YF++ I FGSF+ +N+ + ++
Sbjct: 1196 LRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYG---YQ-TYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-------------------------------------------CVNTTTGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVI 1471
BLAST of Sodium channel protein vs. Ensembl Mouse
Match: Scn1a (sodium channel, voltage-gated, type I, alpha [Source:MGI Symbol;Acc:MGI:98246]) HSP 1 Score: 880.937 bits (2275), Expect = 0.000e+0 Identity = 546/1191 (45.84%), Postives = 767/1191 (64.40%), Query Frame = 2
Query: 2132 INEPEMKKRISALS------VFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWD---------------------------SNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAE-------------VIFWNQPYDCLP--------DSIRCFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDK--------KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
+ +P ++R +++ V E E +K +F+ F W+C P + +K IV+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS+VEL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y K+ + K+PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +D++ ++N LQ A+ R+++ I +VK K+ Y I + +K + +IK P D + ++ I +++ K +E++ +++P L V + ++E F ++ D+ E++ + P + P + CF V+ CC E K WW +R + F+IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ CV++ + E S V N SDC +L ++ E W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY + + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q ++ L +N VF+++F ECV+K+I+LR YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C +K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT FME + LS+F A + PL +PQPNK++L +++P+ G I C+D++ TK LGE
Sbjct: 693 LEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY---KDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDN-EMNNLQIAVDRMHKGIAYVKRKI---YEFIQQSFVKKQKILDEIK---PLDDLNNRKDNCISNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVIEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1867 HSP 2 Score: 319.701 bits (818), Expect = 2.229e-87 Identity = 187/383 (48.83%), Postives = 252/383 (65.80%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK-VFDNSSIGAETYENDSILLWNIKMDYYNNPEN-------WQNPI--TFCHNF---------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ + LEDLDP+Y +K+TF+V+N K IFRFS+T A ++ +PFNPLR AI+IL HSLFS ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK +ARG + FT+LRD WNWLDF VI AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KCV+ N+S+ + E NI MDY N W++ I + H F N+ C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 55 LEAGKNLPFIYGDIPPE--MVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEK------NITMDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 426 HSP 3 Score: 87.8113 bits (216), Expect = 1.505e-16 Identity = 57/187 (30.48%), Postives = 97/187 (51.87%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVD--SNNVRLHESLVQ 4027
+R A KI+ H F I+ I+ + + +T+ + D + ++ FT I+ E ++K A G + +D W WLDFT++ A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV N L E ++
Sbjct: 117 LRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNP--------PDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEF--VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIE 293 HSP 4 Score: 73.559 bits (179), Expect = 3.398e-12 Identity = 76/323 (23.53%), Postives = 144/323 (44.58%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-STCNEEYECIRTDNENPNFGY----TSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR RI+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G YQ TY +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H C N T+ ++ + S N +C++ N + +FDN G L ++ T W + +Y + N ++ +YF++ I FGSF+ +N+ + ++
Sbjct: 1196 LRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYG---YQ-TYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-------------------------------------------CVNTTTGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVI 1471
BLAST of Sodium channel protein vs. Ensembl Mouse
Match: Scn2a (sodium channel, voltage-gated, type II, alpha [Source:MGI Symbol;Acc:MGI:98248]) HSP 1 Score: 862.833 bits (2228), Expect = 0.000e+0 Identity = 532/1141 (46.63%), Postives = 746/1141 (65.38%), Query Frame = 2
Query: 2246 WECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYS-----------------TYNHIGKKLMKIIRRHRQIKYP----FPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAEVIF------------------WNQPYDCLPDSIR---CFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
W+CC + +K +V+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS++EL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + ++ ++PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFSS++L +D++ ++N LQ A+ R+ + I +VK K+ + K I H I+ + D N + I +++ K +E++ +++P L V + ++E +F ++ D+ E++ QP +S+ CF VR CC E K WW +R + +KIV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ C++ + + S+V N S+C L + + W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY +N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q +E+++ L +N VF+++F ECV+K+I+LR YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C +K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F A + PL I +PNK++L +++P+ G I C+D++ TK LGE
Sbjct: 740 WDCCKPWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVSLSLMELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI---SNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDN-EMNNLQIAVGRMQKGIDFVKRKIREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTS-GIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEAEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECQALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1869 HSP 2 Score: 317.005 bits (811), Expect = 1.410e-86 Identity = 185/382 (48.43%), Postives = 253/382 (66.23%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK-VFDNSS--IGAETYENDSI----LLWNIKMDYYNNPE-----------NWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ + LEDLDP+Y +K+TF+V+N K I RFS+T A ++ +PFNP+R AI+IL HSLF+ ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK LARG + FT+LRD WNWLDF VI AY+ +VN LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KC++ DNS+ I ++ N+S+ +N M+ +N E QN C N + C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 56 LEAGKSLPFIYGDIPPE--MVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATSALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVN--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSTFEINITSFFNNSLDWNGTAFNRTMNMFNWDEYIEDKSHFYFLEGQNDALLCGNSSD----AGQCPEGYICVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 428 HSP 3 Score: 84.7297 bits (208), Expect = 1.267e-15 Identity = 51/165 (30.91%), Postives = 89/165 (53.94%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGG 3970
IR A KI+ H F I+ I+ + + +T+ + D + ++ FT I+ E ++K A G + +D W WLDFT++ A ++ F + G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G
Sbjct: 118 IRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNP--------PDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEF--VNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMG 272 HSP 4 Score: 67.0106 bits (162), Expect = 2.966e-10 Identity = 75/323 (23.22%), Postives = 142/323 (43.96%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-STCNEEYECIRTDNENPNFGY----TSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR +I+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G +Y FT +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H C N+T+ + S N EC N + +FDN G+ L ++ T W + +Y + N ++ +YF++ I FGSF+ +N+ + ++
Sbjct: 1198 LRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMY-FT---NAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-------------------------------------------CINYTTGEMFDVSVVNNYSECQALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVI 1473
BLAST of Sodium channel protein vs. UniProt/SwissProt
Match: sp|A2APX8|SCN1A_MOUSE (Sodium channel protein type 1 subunit alpha OS=Mus musculus OX=10090 GN=Scn1a PE=1 SV=1) HSP 1 Score: 882.478 bits (2279), Expect = 0.000e+0 Identity = 577/1350 (42.74%), Postives = 823/1350 (60.96%), Query Frame = 2
Query: 1691 QNLPKSHVSLYLPRTKSLQEISKL-----NVDQKSDIAESKHKESNDN-MLIDS--CPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDD--KRFSDLASCQVNTSKAQH--DSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALS------VFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWD---------------------------SNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAE-------------VIFWNQPYDCLP--------DSIRCFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDK--------KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
Q+L SL+ PR S + +V ++D A+ +H DN DS P H R Q + +++ +S D S + V TS +I++ + + N ++ +R S ++ ++ LE +P ++R +++ V E E +K +F+ F W+C P + +K IV+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS+VEL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y K+ + K+PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS+++L +D++ ++N LQ A+ R+++ I +VK K+ Y I + +K + +IK P D + ++ I +++ K +E++ +++P L V + ++E F ++ D+ E++ + P + P + CF V+ CC E K WW +R + F+IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ CV++ + E S V N SDC +L ++ E W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY + + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q ++ L +N VF+++F ECV+K+I+LR YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C +K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT FME + LS+F A + PL +PQPNK++L +++P+ G I C+D++ TK LGE
Sbjct: 554 QSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDD--NGTTTETEMRKRRSSSFHVSMDFLE-------DPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY---KDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDN-EMNNLQIAVDRMHKGIAYVKRKI---YEFIQQSFVKKQKILDEIK---PLDDLNNRKDNCISNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVIEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1878 HSP 2 Score: 319.701 bits (818), Expect = 1.344e-86 Identity = 187/383 (48.83%), Postives = 252/383 (65.80%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK-VFDNSSIGAETYENDSILLWNIKMDYYNNPEN-------WQNPI--TFCHNF---------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ + LEDLDP+Y +K+TF+V+N K IFRFS+T A ++ +PFNPLR AI+IL HSLFS ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK +ARG + FT+LRD WNWLDF VI AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KCV+ N+S+ + E NI MDY N W++ I + H F N+ C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 55 LEAGKNLPFIYGDIPPE--MVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEK------NITMDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 426 HSP 3 Score: 87.8113 bits (216), Expect = 9.688e-16 Identity = 57/187 (30.48%), Postives = 97/187 (51.87%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVD--SNNVRLHESLVQ 4027
+R A KI+ H F I+ I+ + + +T+ + D + ++ FT I+ E ++K A G + +D W WLDFT++ A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV N L E ++
Sbjct: 117 LRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNP--------PDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEF--VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIE 293 HSP 4 Score: 73.559 bits (179), Expect = 2.281e-11 Identity = 76/323 (23.53%), Postives = 144/323 (44.58%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-STCNEEYECIRTDNENPNFGY----TSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR RI+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G YQ TY +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H C N T+ ++ + S N +C++ N + +FDN G L ++ T W + +Y + N ++ +YF++ I FGSF+ +N+ + ++
Sbjct: 1207 LRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYG---YQ-TYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-------------------------------------------CVNTTTGDIFEISEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVI 1482
BLAST of Sodium channel protein vs. UniProt/SwissProt
Match: sp|P04774|SCN1A_RAT (Sodium channel protein type 1 subunit alpha OS=Rattus norvegicus OX=10116 GN=Scn1a PE=1 SV=1) HSP 1 Score: 881.708 bits (2277), Expect = 0.000e+0 Identity = 571/1350 (42.30%), Postives = 818/1350 (60.59%), Query Frame = 2
Query: 1691 QNLPKSHVSLYLPRTKSLQEISKL-----NVDQKSDIAESKHKESNDN-MLIDS--CPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDD--KRFSDLASCQVNTSKAQH--DSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALS------VFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWD---------------------------SNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAE-------------VIFWNQPYDCLP--------DSIRCFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDK--------KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
Q+L SL+ PR S + +V ++D A+ +H DN DS P H R Q + +++ +S D S + V TS +I++ + + N ++ +R S ++ ++ LE +P ++R +++ V E E +K +F+ F W+C P + +K IV+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS+VEL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y K+ + K+PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++ NLV+LNLFLALLLSSFS+++L +D++ ++N LQ A+ R+++ + +VK K+Y +R+ + + P D + ++ I +++ K +E++ +++P L V + ++E F ++ D+ E++ + P + P + CF V+ CC E K WW +R + F+IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ CV++ E + V N SDC +L ++ E W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY + + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q ++ L +N VF+++F ECV+K+I+LR YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C +K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT FME + LS+F A + PL +PQPNK++L +++P+ G I C+D++ TK LGE
Sbjct: 554 QSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDD--NGTTTETEMRKRRSSSFHVSMDFLE-------DPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSY---KDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIRNLVVLNLFLALLLSSFSADNLAATDDDN-EMNNLQIAVDRMHKGVAYVKRKIYEFIQQ------SFVRKQKILDEIKPLDDLNNRKDNCTSNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVMEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDTFEITEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1878 HSP 2 Score: 316.62 bits (810), Expect = 1.116e-85 Identity = 185/383 (48.30%), Postives = 251/383 (65.54%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK-VFDNSSIGAETYENDSILLWNIKMDYYNNPEN-------WQNPI--TFCHNF---------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ + LEDLDP+Y +K+TF+V+N K IFRFS+T A ++ +PFNPLR AI+IL HSLFS ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK +ARG + FT+LRD WNWLDF VI AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KCV+ N+S+ + E N+ DY N W++ I + H F N+ C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 55 LEAGKNLPFIYGDIPPE--MVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEK------NVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 426 HSP 3 Score: 87.8113 bits (216), Expect = 9.134e-16 Identity = 57/187 (30.48%), Postives = 97/187 (51.87%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVD--SNNVRLHESLVQ 4027
+R A KI+ H F I+ I+ + + +T+ + D + ++ FT I+ E ++K A G + +D W WLDFT++ A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV N L E ++
Sbjct: 117 LRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNP--------PDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEF--VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIE 293 HSP 4 Score: 73.559 bits (179), Expect = 2.320e-11 Identity = 79/318 (24.84%), Postives = 143/318 (44.97%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR RI+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G YQ TY +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H CV + N S L K+ N W+N +K +FDN G L ++ T W + +Y + N ++ +YF++ I FGSF+ +N+ + ++
Sbjct: 1207 LRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYG---YQ-TYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-CVNTTTGDTFEITEVNNHSDCL---KLIERNETARWKN-------------VK---------------------VNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVI 1482
BLAST of Sodium channel protein vs. UniProt/SwissProt
Match: sp|A2ASI5|SCN3A_MOUSE (Sodium channel protein type 3 subunit alpha OS=Mus musculus OX=10090 GN=Scn3a PE=1 SV=1) HSP 1 Score: 862.833 bits (2228), Expect = 0.000e+0 Identity = 550/1248 (44.07%), Postives = 779/1248 (62.42%), Query Frame = 2
Query: 1952 FSDLASCQVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISN--IVLEDLEGLYNNINEPEMKKRISALSVF-----ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYN------------HIGKKL-------MKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE-----------------------------------PKFDADPDINEAEVIFWNQPYDCLPDS-IRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
F+D S+++ DSL + H+ + N + R+ R+S+ I +E LE + ++ +S S+ E E +K RF F W+CC ++ +K +V+ IV+DPF++L ITICIVLNT+FM+++ Y + ++ NL F IFT E +K+IA P YF E WN+FD +IV LS++EL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + ++ K+PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFSS++L +D++ ++N LQ A+ R+ + I +VK K+ + H G K+ ++ +++ Y + S + +++ K +E + +++P L V + ++E P+ +I E + +P C + I+ F C +E K WW +R + + IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ CV+ + + S V N SDC L K W N ++NFDNV GYL+L Q+ATFKGW DIM AVD +V QP Y N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q K ++ L +N VF+++F E ++K+I+LR+YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C D K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F A + PL I +PNK++L +++P+ G I C+D++ TK LGE
Sbjct: 593 FADDEHSTFEDSESRRDSLFVPHRPGERR-NSNGTTTETEVRKRRLSSYQISMEMLE------DSSGRQRAMSIASILTNTMEELEESRQKCPPCWYRFANVFLIWDCCDSWLKVKHLVNLIVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLTVGNLVFTGIFTAEMVLKIIAMDPYYYFQEGWNIFDGIIVSLSLMELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI---NEDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLIVFMLVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDN-EMNNLQIAVGRMQKGIDYVKNKIRECFRKAFFRKPKVIEIHEGNKIDSCMSNNTGVVEISKELNYLKDGNGTTSG-VGTGSSVEKYVIDENDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESELEESKEKLNATSSSEGSTVDVAPPREGEQAEIEPEEDL---KPEACFTEGCIKKF--PFCQVSTEEGKGKIWWNLRKTCYSIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQTYFTNAWCWLDFLIVDVSLVSLVANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNMTTGSMFDMSEVNNFSDCQALGK--QARWKNVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRDVKLQPVYEENLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSKYMTLVLSRINLVFIVLFTGEFLLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSAPPDCDPDAIHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1815 HSP 2 Score: 315.079 bits (806), Expect = 3.033e-85 Identity = 183/382 (47.91%), Postives = 250/382 (65.45%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSS---IGAETYENDSI----LLWNIKMDYYNNPENWQNPITFCHNF-----------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ + LEDLDP+Y K+TFVV+N K IFRFS+T A ++ +P NP+R AI+IL HSLFS ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK LARG + FT+LRD WNWLDF VI +AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KC++ + S I +Y N ++ N+ M +N W++ I +F N C E Y C++ NPN+GYTSFD F + L FRLMTQDYWENLYQL +R G +MI+F++VIF GSFYL+N+IL++VAM+Y
Sbjct: 55 LEAGKNLPFIYGDIPPE--MVSEPLEDLDPYYVSKKTFVVLNKGKAIFRFSATSALYILTPLNPVRKIAIKILVHSLFSMLIMCTILTNCVFMTLSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFSVIVMAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPSDSAFEINTTSYFNGTMDSNGTFVNVTMSTFN----WKDYIADDSHFYVLDGQKDPLLCGNGSDAGQCPEGYICVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDYWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLVNLILAVVAMAY 427 HSP 3 Score: 89.3521 bits (220), Expect = 3.683e-16 Identity = 62/234 (26.50%), Postives = 115/234 (49.15%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVL--NGYLSLFQIATFKGWTDIMAD 4171
+R A KI+ H F I+ I+ + + +TL + D + ++ FT I+ E ++K A G + +D W WLDF+++++A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G ++ C + +++ +N+ F+ + NG ++TF W D +AD
Sbjct: 117 VRKIAIKILVHSLFSMLIMCTILTNCVFMTLSNP--------PDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFSVIVMAYVTEF--VDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNL------------------RNKCLQWPPSDSAFEINTTSYFNGTMDSNGTFVNVTMSTFN-WKDYIAD 321 HSP 4 Score: 66.6254 bits (161), Expect = 3.092e-9 Identity = 77/319 (24.14%), Postives = 143/319 (44.83%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHM-----------IYFMMVIFFGSFYLINVILSIV 1474
LR I+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G +Q TY +AW WLDF+++ ++ + V +A LG + +LR+LR LR L+ ++ GM+ VV++L+ AI + + L++ + IF+++G+ ++ G H CV + S ++ +NF S C + R N N FDN G L ++ T W ++ + + DV + +YF++ I FGSF+ +N+ + ++
Sbjct: 1147 LRKTCYSIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYG---FQ-TYFTNAWCWLDFLIVDVSLVSLVANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-CVNMTTGSMFD----------------------------MSEVNNF-------SDCQALGKQARWKNVKVN-----FDNVGAGYLALLQVATFKGWMDIMYAAVDSR-DVKLQPVYEENLYMYLYFVIFIIFGSFFTLNLFIGVI 1419
BLAST of Sodium channel protein vs. UniProt/SwissProt
Match: sp|B1AWN6|SCN2A_MOUSE (Sodium channel protein type 2 subunit alpha OS=Mus musculus OX=10090 GN=Scn2a PE=1 SV=1) HSP 1 Score: 862.833 bits (2228), Expect = 0.000e+0 Identity = 532/1141 (46.63%), Postives = 746/1141 (65.38%), Query Frame = 2
Query: 2246 WECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYS-----------------TYNHIGKKLMKIIRRHRQIKYP----FPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAEVIF------------------WNQPYDCLPDSIR---CFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
W+CC + +K +V+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS++EL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + ++ ++PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFSS++L +D++ ++N LQ A+ R+ + I +VK K+ + K I H I+ + D N + I +++ K +E++ +++P L V + ++E +F ++ D+ E++ QP +S+ CF VR CC E K WW +R + +KIV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ C++ + + S+V N S+C L + + W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY +N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q +E+++ L +N VF+++F ECV+K+I+LR YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C +K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F A + PL I +PNK++L +++P+ G I C+D++ TK LGE
Sbjct: 740 WDCCKPWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVSLSLMELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI---SNDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDN-EMNNLQIAVGRMQKGIDFVKRKIREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTS-GIGSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEAEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECQALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1869 HSP 2 Score: 317.005 bits (811), Expect = 9.866e-86 Identity = 185/382 (48.43%), Postives = 253/382 (66.23%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK-VFDNSS--IGAETYENDSI----LLWNIKMDYYNNPE-----------NWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ + LEDLDP+Y +K+TF+V+N K I RFS+T A ++ +PFNP+R AI+IL HSLF+ ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK LARG + FT+LRD WNWLDF VI AY+ +VN LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KC++ DNS+ I ++ N+S+ +N M+ +N E QN C N + C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 56 LEAGKSLPFIYGDIPPE--MVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATSALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVN--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSTFEINITSFFNNSLDWNGTAFNRTMNMFNWDEYIEDKSHFYFLEGQNDALLCGNSSD----AGQCPEGYICVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 428 HSP 3 Score: 84.7297 bits (208), Expect = 8.864e-15 Identity = 51/165 (30.91%), Postives = 89/165 (53.94%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGG 3970
IR A KI+ H F I+ I+ + + +T+ + D + ++ FT I+ E ++K A G + +D W WLDFT++ A ++ F + G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G
Sbjct: 118 IRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNP--------PDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEF--VNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMG 272 HSP 4 Score: 67.0106 bits (162), Expect = 2.075e-9 Identity = 75/323 (23.22%), Postives = 142/323 (43.96%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-STCNEEYECIRTDNENPNFGY----TSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR +I+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G +Y FT +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H C N+T+ + S N EC N + +FDN G+ L ++ T W + +Y + N ++ +YF++ I FGSF+ +N+ + ++
Sbjct: 1198 LRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMY-FT---NAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-------------------------------------------CINYTTGEMFDVSVVNNYSECQALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVI 1473
BLAST of Sodium channel protein vs. UniProt/SwissProt
Match: sp|P04775|SCN2A_RAT (Sodium channel protein type 2 subunit alpha OS=Rattus norvegicus OX=10116 GN=Scn2a PE=1 SV=1) HSP 1 Score: 862.448 bits (2227), Expect = 0.000e+0 Identity = 539/1184 (45.52%), Postives = 760/1184 (64.19%), Query Frame = 2
Query: 2132 INEPEMKKRISALSVF-----ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYS-----------------TYNHIGKKLMKIIRRHRQIKYP----FPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAEVIF------------------WNQPYDCLPDSIR---CFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
+ +P ++ +S S+ E E +K +F W+CC + +K +V+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS++EL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + ++ ++PRW+ F HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFSS++L +D++ ++N LQ A+ R+ + I +VK K+ + K I H I+ + D N + I +++ K +E++ +++P L V L ++E +F ++ D+ E++ QP +S+ CF VR CC E K WW +R + +KIV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ C++ + + S+V N S+C L + + W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY +N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q +E+++ L +N VF+++F ECV+K+I+LR YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C +K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F A + PL I +PNK++L +++P+ G I C+D++ TK LGE
Sbjct: 696 LEDPSRQRAMSMASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSSVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVSLSLMELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI---SNDCELPRWHMHHFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDN-EMNNLQIAVGRMQKGIDFVKRKIREFIQKAFVRKQKALDEIKPLEDLNNKKDSCISNHTTIEIGKDLNYLKDGNGTTS-GIGSSVEKYVVDESDYMSFINNPSLTVTVPIALGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEAEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNLRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECQALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFCKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1868 HSP 2 Score: 314.694 bits (805), Expect = 5.284e-85 Identity = 186/383 (48.56%), Postives = 252/383 (65.80%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK-VFDNSS--IGAETYENDSILLWN-IKMDYYNNPENW---------------QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ + LEDLDP+Y +K+TF+V+N K I RFS+T A ++ +PFNP+R AI+IL HSLF+ ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK LARG + FT+LR+ WNWLDF VI AY+ +VN LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KC++ DNS+ I ++ N+S L WN + N NW QN C N + C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 56 LEAGKSLPFIYGDIPPE--MVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATSALYILTPFNPIRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRNPWNWLDFTVITFAYVTEFVN--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCLQWPPDNSTFEINITSFFNNS-LDWNGTAFNRTVNMFNWDEYIEDKSHFYFLEGQNDALLCGNSSD----AGQCPEGYICVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAY 428 HSP 3 Score: 82.8037 bits (203), Expect = 3.437e-14 Identity = 50/165 (30.30%), Postives = 89/165 (53.94%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGG 3970
IR A KI+ H F I+ I+ + + +T+ + D + ++ FT I+ E ++K A G + ++ W WLDFT++ A ++ F + G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G
Sbjct: 118 IRKLAIKILVHSLFNVLIMCTILTNCVFMTMSNP--------PDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRNPWNWLDFTVITFAYVTEF--VNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMG 272 HSP 4 Score: 67.0106 bits (162), Expect = 2.057e-9 Identity = 75/323 (23.22%), Postives = 142/323 (43.96%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-STCNEEYECIRTDNENPNFGY----TSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVH---------MIYFMMVIFFGSFYLINVILSIV 1474
LR +I+ H+ F F++ I+++ A+ T + E + +FT I+ E ++K +A G +Y FT +AW WLDF+++ ++ + +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G H C N+T+ + S N EC N + +FDN G+ L ++ T W + +Y + N ++ +YF++ I FGSF+ +N+ + ++
Sbjct: 1197 LRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMY-FT---NAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYH-------------------------------------------CINYTTGEMFDVSVVNNYSECQALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVI 1472
BLAST of Sodium channel protein vs. TrEMBL
Match: O02037 (Sodium channel protein OS=Bdelloura candida OX=46766 GN=BdNa1 PE=2 SV=1) HSP 1 Score: 1214.91 bits (3142), Expect = 0.000e+0 Identity = 690/1208 (57.12%), Postives = 894/1208 (74.01%), Query Frame = 2
Query: 2042 NKVDSKVNFIE--RESRISNIVLEDLEG--LYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETE---PSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY--FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCV-DSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE----FNSGNPMMDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKK 5623
++++ K++ IE + ++NI L D E ++ NE K+R + ++E + K +R ++ + C+W+ CPAY +K VSFI+LDPF+ELFIT+CIV NT FM+++ S+ N FFM +FTFEA K IA G + YFSE+WNVFDF++V++S++EL S G +S+LR FRLLR+FKLAKSWQTMNLLFTIIG T+GAIGNLCIVL IIVFIFAV+GM F Y++++N T +P+ G +PRWNF DF +SFMIVFRVLCGEY+ESMWDC+RVN CIPFFL TMILGNLVILNLFLALL+SSFSSESL+RRE+ES ++NKLQ+A RVYRA+V++K K + I K+L+ + R YP PW+ + ++ N ++ PS ++ PQ Q P FD P+I +AE+++WN P DCL +L R+C CC + + WW IRC FK+VDHK+FETFIL MI SSLT+TLEDK+LP L++ + +D FT++FL+EM+LKW AIGL KYFKD W LDF IV++ALIS F + A KA+RTLRALRPLRA+SRW+GM+IVVNALIQAIPSIFNV++VCLVFWLIFSIIG+Q FGG+F+KC+ + ++ +V +K C N++W+NS+I+FD+V+NG L+LFQ+ATFKGWTDI+ D+VDVT+++ QP++NN+ YIY+VLFIIFG+FFTLNLFIGVIIDNFNVQKKK+GGSLE+FMTD QK+YY+A++KMSS SPQKP+P+PK+ I+ +FYNI N+ FDIVIM FI+LNT+ MC+E Q K L+ LD + +VF+LIFA E ++K+I RW+YFK PWNVFDF +V+FS W+++ F + PTLIR++R+FRI RVLRLVKSARGIRTLLFSLVVSLPALFNI LLLML+MFIYAI+G+S F+ V + YGID TFNFETF +MIILFQIST+GGW+S++DGLMNN + EC CGS +LA YL+SYL+I+ VI+NMYIAVILENFSQATKD Q+GLTQDDYDLFYE WE+YDP ATGF+ L DL +FV +IG+PLGI +PNKI+LA+LEIPIC+G+ ICC+D++D LTKN+L E F+ ++DLKP K+ I KK +ISNT+ R WAARKIT +R + R K
Sbjct: 505 SQIEQKLDNIEINESTSMTNITLGDSEKEKSMDDNNEKIQKRRAAVFEIYEEKFKKSQRQLFFEMVEAKMCSWQFCPAYIKFQKCVSFIILDPFLELFITLCIVCNTCFMALEGPFTEESVKIFFRHSNYFFMGVFTFEAIGKQIALGLKTYFSERWNVFDFIVVIISLLEL------SRPNGVMSILRAFRLLRVFKLAKSWQTMNLLFTIIGHTVGAIGNLCIVLGIIVFIFAVLGMNTFHYGYQKYRNTTLYPEFEGHLPRWNFDDFYNSFMIVFRVLCGEYIESMWDCLRVNNYLCIPFFLLTMILGNLVILNLFLALLISSFSSESLERREEESIEINKLQQAFNRVYRAVVFIKYKFVKLFKLIKKRLL-MYSWFRPALYPDPWNEFIEKKGTARTKVLFPNYDDNAWKWPS-DLQIPQEQQ-----------PSFDFIPEITDAEIVYWNMPLDCLSRDAVDYLNRNCPCCFRNRYYRVWWRIRCLTFKVVDHKYFETFILFMIAFSSLTMTLEDKYLPNYPMLENTIYILDKVFTIVFLMEMLLKWMAIGLYKYFKDPWSCLDFAIVMIALISLMPFDMSRYDALKAMRTLRALRPLRAISRWEGMRIVVNALIQAIPSIFNVMVVCLVFWLIFSIIGVQRFGGRFYKCIYKDSKSQVSPLIVNSKIACETFANQLNITWINSKIHFDDVVNGLLALFQVATFKGWTDIIKDSVDVTDIDKQPRHNNSRINYIYYVLFIIFGSFFTLNLFIGVIIDNFNVQKKKVGGSLEMFMTDDQKKYYNAIQKMSSSSPQKPIPKPKNHISIVFYNICKNKNFDIVIMGFIVLNTLTMCIEFQDQPKLLTRVLDIVGRVFLLIFAGEFIMKLIGFRWFYFKDPWNVFDFSVVVFSTIAWILQFFESIFPLDPTLIRIVRLFRITRVLRLVKSARGIRTLLFSLVVSLPALFNIALLLMLVMFIYAIMGLSFFANVPHKYGIDETFNFETFFNSMIILFQISTTGGWHSVMDGLMNNESPECSEIPSPCGSKLLASSYLISYLIINAFVIVNMYIAVILENFSQATKDFQMGLTQDDYDLFYEVWEKYDPEATGFIPLSDLYDFVDQIGQPLGIAKPNKIQLAILEIPICQGNNICCMDVLDILTKNYLAESTKTFDPSVGVVDLKPTVKSTIADKKLIISNTYENLRAEWAARKITNLMRNFILRCK 1693 HSP 2 Score: 413.69 bits (1062), Expect = 1.662e-114 Identity = 283/547 (51.74%), Postives = 373/547 (68.19%), Query Frame = 2
Query: 206 MKEIYNKYRILTEESLNQANILYXXXXXXXXXXXXXXXXXXXQRXXXXXXXXXXXXXXXXXXXXDKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSL 1846
MKE+ N++R LT ES+ + NI Y ES++ +KEEK KI+ K K K +S PD RLE GK L LGD F KKKF G++++++DP+Y DKR+FVVIN SK I+RF++TKAFFLFSPFNP+R +AI+ILTH LFS VL TII NCI M N + + +E FT IY AEF++KA ARGLI+ FTYLR WNWLDF+VIGLAY+M++ LG S+LR+LRV RALKTVT +PGM TVV++LL ++IKL+DA+VLT+FML+IFALIGLQ+Y+GTL KCVK FDNS++ + EN ++ +IK+ +YN+PENW P C N T + C Y C T NPNFG+T++D F + LCAFRLMTQD+WE+LYQ IR NG +H+ +FM VIFFGSFYL+NVIL+IVAMSYD+ R+ D A+ EEEA+KK EEE + LE + D S+DL F AI + + +KSC DI ++ L+ R+ SL S N D S + +S+ ++ DN+ I+ S+
Sbjct: 1 MKEVVNRFRFLTWESVKEGNIRYVESVRRKKEEK--------AEKINLDKKGKSKDKKASTLAPDPRLEEGKKLSFLLGDCFSKKKFGGQLIDEIDPYYYDKRSFVVINKSKNIYRFTTTKAFFLFSPFNPVRTSAIKILTHPLFSIVVLLTIITNCIIMALNLDIKALEQSFTIIYAAEFLVKAFARGLIINDFTYLRSIWNWLDFLVIGLAYLMFIEPDLGNYSSLRTLRVFRALKTVTILPGMTTVVNALLNSVIKLKDAIVLTLFMLAIFALIGLQLYKGTLTQKCVKEFDNSTMNLD--ENSTVAWDDIKI-FYNSPENWHEPHKVCSNST------APCPPGYLCFATSEGNPNFGFTNYDTFPWAYLCAFRLMTQDFWEDLYQNTIRANGKIHVFFFMFVIFFGSFYLVNVILAIVAMSYDEARLKDFAKTAEEEAQKKEEEELMMEALE---EDFDLYSEDLYFKAIVTNEEVESKS-LKSCDDIYQPICTENLAQLF--RSNSLNFYS--NKDGASCLDKSQIEQKLDNIEINESTSM 522 HSP 3 Score: 89.3521 bits (220), Expect = 1.061e-13 Identity = 56/185 (30.27%), Postives = 100/185 (54.05%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV---DSNNVRLHES 4018
+R SA KI+ H F +L+ I+ + + + L ++ ++ FT+I+ E ++K FA GL Y + W WLDF ++ +A + + + D G + ++RTLR R L+ V+ GM VVNAL+ ++ + + +++ L IF++IG+QL+ G KCV D++ + L E+
Sbjct: 124 VRTSAIKILTHPLFSIVVLLTIITNCIIMALNLD-----------IKALEQSFTIIYAAEFLVKAFARGLIINDFTYLRSIWNWLDFLVIGLAYLMFIEP-DLGNYSSLRTLRVFRALKTVTILPGMTTVVNALLNSVIKLKDAIVLTLFMLAIFALIGLQLYKGTLTQKCVKEFDNSTMNLDEN 296 HSP 4 Score: 64.3142 bits (155), Expect = 3.650e-6 Identity = 70/321 (21.81%), Postives = 138/321 (42.99%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSES-------ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIP---GMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCV---------KVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLII----------RTNGDVHMIYFMMVIFFGSFYLINVILSIV 1474
+R +++ H F F+L I + + MT + EN I ++T F+++ L + + + + Y +D W+ LDF ++ +A + + + + AL+++R LRAL+ + I GM+ VV++L+ AI + + +V+ + IF++IG+Q + G +KC+ + NS I ET+ N + W I S + FD+ LL F++ T W ++ + + N ++ IY+++ I FGSF+ +N+ + ++
Sbjct: 975 IRCLTFKVVDHKYFETFILFMIAFSSLTMTLEDKYLPNYPMLENTIYILDKVFTIVFLMEMLLKWMAIGLYKYFKDPWSCLDFAIVMIALISLMPFDMSRYDALKAMRTLRALRPLRAISRWEGMRIVVNALIQAIPSIFNVMVVCLVFWLIFSIIGVQRFGGR-FYKCIYKDSKSQVSPLIVNSKIACETFANQLNITW----------------------------INSKIH-------------------FDDVVNGLLALFQVATFKGWTDIIKDSVDVTDIDKQPRHNNSRINYIYYVLFIIFGSFFTLNLFIGVI 1247
BLAST of Sodium channel protein vs. TrEMBL
Match: A0A1I8HM84 (Sodium channel protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 1209.13 bits (3127), Expect = 0.000e+0 Identity = 703/1322 (53.18%), Postives = 903/1322 (68.31%), Query Frame = 2
Query: 1856 NRFKVYQQFNVF--RKLIEDN---RKESSKYSLDDKRFSDLASCQVNTSKAQHDSLILNHQLSSNK---------INKVDSKVNFIERESRI---SNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFC-NWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNH-IGKKLMKII--------------------------RRHRQIKYPFPWDSNESNPI-NIQAAIIKM---NKNETEPSRNVHDPQLNQV----AFNLWLEN-YEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYF-------KVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKT-ENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK---CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKKE 5626
++FK + F+ F R+ + + + +SK L+ +++ D+ C N S ++L + I K S+ E S+ + + D+ L I + K EC K +K + Y F + FC +WECC + L+K VS+ +LDPF+ELFIT+CI++NT M++D N+ + I++ NLFF F EA KLIA P++YF + WN+FDF IVVLS VELT + +G LS+LR FRLLR+FKLAKSWQTMNLLF II RT+GA+GNL VL II+FIFAVVGMQ+FG Y +KN+T +P+ G PRWNF DFTHSFMIVFRVLCGE++ESMW C+ VNG C+PFFL T IL +LV+L+LFLALLLSSF +ESLQRRE+E +VNKLQEA+ R+ R IVW ++ + + + +GK + R Q+ FP + + + N+ A +M + NE + N+V F + +E+ PKFD +P+INE ++ +W+ P DCLP + F R C + + K WW RC A+K+V+HK+FE FI++MI SS+TLTLED LP R +LK +LE+MD FT+IF EMV+KWFA GL KYFKDAWCWLDF IV VAL S + GAFKA+RTLRALRPLRAVSRW+GMK+VVNALIQAIPSIFNVLLVCLVFWLIFSI+G+QLFGG+F+KCVDSNN R+ LV NK C + NVSW NS+INFDN LNGYL+LFQIAT+KGW I+ADAVD+T N QP + +T Y++FVLFIIFG+FFTL LFIGVIIDNFNVQKK+ GGS+ELFMT+ QKRYY AMKKMSS +PQKPVP+PK I Q F+ +IN+QKFDI IM FIMLNTIVMC+E H Q + +DA++ +N+ F++IF ECV+K++A RWYYFK WN+FDF+IV+ SI W IE ++ + PT+IRV+RVFR+GRVLRLVKSARGIRTLLFSL VSLPALFNIGLLL +IMFIY+I+G+S FS V + GID FNFETF M++LFQ+STS GW+S+LDGL+N +C +KK CG+Y +AV++L+SYLVISFLVIINMYIAVILENFSQAT+DVQ GLTQ+D+DLFYE WE YDP A GF+ + + EF+ ++ PL +PQPNKI+LA L +PICKG TI CVDL+D LT+NFLG + LKPK K+ ++ +ISNT QR+ +AA IT ++R A+ KE
Sbjct: 618 SKFKYFALFSAFANRQPTKTDLLGKIANSKSPLESRKYKDILMCTRNGSDKPIPEILLQEETKQPGVLDHKELLGIRKTQSQRTLRETTSQPPAGQEVEMNDVVVLKTLIEDAATTK--PEKEEVECRPKEKKFEHYKHLFLEKFCYSWECCYCWKVLEKYVSYFILDPFVELFITLCILVNTACMALDVPSNTPTFKEILSNANLFFTATFGIEAVCKLIAMKPQVYFKDGWNIFDFTIVVLSFVELT----LQNVQG-LSILRAFRLLRVFKLAKSWQTMNLLFAIIARTMGALGNLVFVLGIIIFIFAVVGMQLFGENYALYKNETLYPEFKGDYPRWNFKDFTHSFMIVFRVLCGEWIESMWGCINVNGYICVPFFLLTNILASLVVLSLFLALLLSSFGAESLQRREEEE-EVNKLQEAIDRINRFIVWSRIHIKRLFWYLVGKACTRSYNDNDLFESDDADDSSEDKAKPASGKDRISIQLDSRFPLKQHGTYIMENVTIAASQMVFKDINEASILSDAAGANQNKVFPAGHFAIAIEDKLSPKFDEEPEINEVDIQYWDAPEDCLPKQLMDFCPRAVQLCDNTNLGKIWWGFRCRAYKLVEHKYFEMFIILMIALSSVTLTLEDVNLPKRPKLKLVLEYMDKCFTIIFTFEMVVKWFAFGLKKYFKDAWCWLDFVIVNVALFSLVLNLVGGASGQNMGAFKAMRTLRALRPLRAVSRWEGMKVVVNALIQAIPSIFNVLLVCLVFWLIFSIMGVQLFGGQFYKCVDSNNERVSADLVPNKEACEAMAAAFHNVSWENSRINFDNFLNGYLALFQIATYKGWIPIIADAVDITGPNNQPIRDASTSMYLFFVLFIIFGSFFTLKLFIGVIIDNFNVQKKRAGGSVELFMTEDQKRYYKAMKKMSSTAPQKPVPKPKLRIPQFFFKVINDQKFDIAIMVFIMLNTIVMCIEHHNQSEAFNDAVEQINRFFIVIFTGECVMKLLAQRWYYFKFAWNMFDFVIVVLSILTWAIEDIMVRLPIPPTMIRVVRVFRVGRVLRLVKSARGIRTLLFSLFVSLPALFNIGLLLFMIMFIYSIVGISFFSHVRHRSGIDEIFNFETFPSGMVVLFQLSTSAGWDSVLDGLINEEEPDCNPNKKPHSDCGNYAIAVVFLISYLVISFLVIINMYIAVILENFSQATEDVQQGLTQEDFDLFYEKWESYDPKAKGFIAVDQVYEFLDELEPPLQLPQPNKIKLATLSVPICKGDTIFCVDLLDALTRNFLGTAADEEELSALKPKGGGKLKDERIIISNTMERQRQKYAANVITRYVRAYAAKCKE 1931 HSP 2 Score: 401.364 bits (1030), Expect = 9.548e-110 Identity = 216/372 (58.06%), Postives = 269/372 (72.31%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVF------DNSSIG-AETYENDSI--LLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
+ +L AG LP L D F K+ G +LED+D +Y DK TFVV++ KTIFRFS+T A FL SPFNP+R AIRILTH FS VL II NC+ M +NS SE VE FTAIYT E IKA++RGLI+ +FTYLRDAWNWLDFIVIGLAY+M+ LG LSALRSLRVLRALKTVTT+PG+KT+V +L+ A+ +LRD +LT+F+LSIFAL+GLQ+Y+GTLL KCV F D +G A +D + L ++ ++NN NW + N+ + C E +EC RTD NPNFGYT+FDNFG S+LCAFRLMTQD WE+LYQLIIRTNG H+++F++VI GSF+LINVIL+IVAM+Y
Sbjct: 87 NPKLAAGMKLPPALSDKFSAKRLGGALLEDIDDYYGDKFTFVVVSRDKTIFRFSATNALFLLSPFNPIRRVAIRILTHPAFSMLVLLAIICNCVFMAQNSSSEAVEYTFTAIYTTEATIKAISRGLILTEFTYLRDAWNWLDFIVIGLAYIMFFVPTLGNLSALRSLRVLRALKTVTTVPGLKTIVGALMEAVKRLRDVGILTIFVLSIFALVGLQVYKGTLLQKCVSKFNFTEYLDYEYMGNASELTSDELKDLKDQVRKIHHNNESNWLGYPDELRVCSLNSDLGQQCPEGFECFRTDKVNPNFGYTNFDNFGWSMLCAFRLMTQDAWESLYQLIIRTNGTPHVVFFVLVIMMGSFFLINVILAIVAMAY 458 HSP 3 Score: 84.7297 bits (208), Expect = 2.961e-12 Identity = 53/176 (30.11%), Postives = 91/176 (51.70%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVDSNN 4000
IR A +I+ H F +L+ I+ + + + E ++ FT I+ E +K + GL Y +DAW WLDF ++ +A I +F V G A+R+LR LR L+ V+ G+K +V AL++A+ + +V ++ + IF+++G+Q++ G KCV N
Sbjct: 154 IRRVAIRILTHPAFSMLVLLAIICNCVFMAQNSS-----------SEAVEYTFTAIYTTEATIKAISRGLILTEFTYLRDAWNWLDFIVIGLAYIMFF-VPTLGNLSALRSLRVLRALKTVTTVPGLKTIVGALMEAVKRLRDVGILTIFVLSIFALVGLQVYKGTLLQKCVSKFN 317 HSP 4 Score: 71.633 bits (174), Expect = 2.184e-8 Identity = 76/320 (23.75%), Postives = 136/320 (42.50%), Query Frame = 2
Query: 602 RAAAIRILTHSLFSFFVLTTIIINCIAMTKNSES-----------ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYV--------NSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHM----------IYFMMVIFFGSFYLINVILSIV 1474
R A +++ H F F++ I ++ + +T + E ++ FT I+T E ++K A GL Y +DAW WLDF+++ +A V +G A+R+LR LR L+ V+ GMK VV++L+ AI + + L++ + IF+++G+Q++ G +KCV +N + A D N E + HN + N +FDNF L F++ T W + + G + ++F++ I FGSF+ + + + ++
Sbjct: 1209 RCRAYKLVEHKYFEMFIILMIALSSVTLTLEDVNLPKRPKLKLVLEYMDKCFTIIFTFEMVVKWFAFGLK----KYFKDAWCWLDFVIVNVALFSLVLNLVGGASGQNMGAFKAMRTLRALRPLRAVSRWEGMKVVVNALIQAIPSIFNVLLVCLVFWLIFSIMGVQLFGGQ-FYKCVDS-NNERVSA---------------DLVPNKEACEAMAAAFHNVSWEN----------------------SRINFDNFLNGYLALFQIATYKGWIPIIADAVDITGPNNQPIRDASTSMYLFFVLFIIFGSFFTLKLFIGVI 1485
BLAST of Sodium channel protein vs. TrEMBL
Match: G3TWG4 (Sodium channel protein OS=Loxodonta africana OX=9785 GN=SCN2A PE=3 SV=1) HSP 1 Score: 1162.9 bits (3007), Expect = 0.000e+0 Identity = 765/1796 (42.59%), Postives = 1098/1796 (61.14%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAE-TYENDSILLWNIKMDYYNNPENW-----QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQ--------------NLPK----------SHVSL-------YLPRTKSLQEISKL-----NVDQKSDIAESKHKESNDNMLIDS------CPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDD--KRFSDLASCQVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVF------ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMK-IIRRHRQIKYPFPWDSNESN--PINIQAAIIKMNKNETEPSRNVHDPQLN-QVAFNLWLENYE----PKFDADPDINEAEVIF------------------WNQPYDCLPDSIR---CFL---VRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE-SLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
LEAGK LP GD P+ + LEDLDP+Y +K+TF+V+N K I RFS+T A ++ +PFNP+R AI+IL HSLF+ ++ TI+ NC+ MT ++ ++NVE FT IYT E +IK LARG + FT+LRD WNWLDF VI AY+ +VN LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +K + F +SS+ T N ++ ++N +Y + ++ QN C N + C E Y C++ NPN+GYTSFD F + L FRLMTQD+WENLYQL +R G +MI+F++VIF GSFYLIN+IL++VAM+Y++ A + E E++EAE + Q+LE L + + + F + + KS + I+ LPK +H+SL + PR S + ++ ++D A+ +H DN DS P H R Q + +++ +S D S + TS L+ L + + ++ +R S ++ ++ LE +P K+R +++ E E +K +F W+CC + +K +V+ +V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF E WN+FD IV LS++EL A+ LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + + ++PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFSS++L +D++ ++N LQ A+ R+ + I +VK +I + + K +R+ + + DS SN + I +++ K +E++ +++P L V + ++E +F ++ D+ E++ QP +S+ CF VR CC E K WW +R + +KIV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM+LKW A G YF +AWCWLDF IV V+L+S + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF+ C++ + + S+V N S+C L + + W N ++NFDNV GYLSL Q+ATFKGW DIM AVD V QPKY +N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKK GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + ++ + Q FDI IM I LN + M +E+ Q +E+++ L +N VF+++F ECV+K+I+LR+YYF I WN+FDF++VI SI + + + VSPTL RVIR+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C +K CG+ + + + VSY++ISFLV++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F A + PL I +PNK++L +++P+ G I C+D++ TK LGE
Sbjct: 56 LEAGKSLPFIYGDIPPE--MVSEPLEDLDPYYINKKTFIVLNKGKAISRFSATPALYILTPFNPIRKLAIKILIHSLFNVLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKILARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVN--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKYITSFFSSSLDVNGTIINRTVSMFNWD-EYIEDKSHFYFLEGQNDALLCGNSSD----AGQCPEGYICVKA-GRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQ-------QMLEQLKKQQEEAQVESTFTVLKSSSVVSKLRFRKSESEDSIRRKGFRFSLEWLLEAKLPKDRQENENQGMNHISLLSIRGSLFSPRRNSRASLFSFRGRAKDIGSENDFADDEHSTFEDN---DSRRDSLFVPHRHGERRHSNVSQASRASRVLPILPMNGKMHSAVDCNGVVSLVGGPSALTSPIGQ--LLPEDLLQTGANSGTTTETEIRKRRSSSYHVSMDLLE-------DPTAKQRAMSMASILTNTMEELEESRQKCPPCWYKFANMCLIWDCCKPWLKVKHLVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEQFSNVLSVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVSLSLMELGL-----ANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKI---SSDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQTMCLTVFMMVMVIGNLVVLNLFLALLLSSFSSDNLAATDDDN-EMNNLQIAVGRMQKGIDYVK-------RNIREFIQKAFVRKQKALDLNNKKDSCISNHTTVEISSSVEKYVVDESDYMSFINNPSLTVTVPIAVGESDFENLNTEEFSSESDMEESKEKLNATSSSEGSTVDIGAPAEGEQPEAEPEESLEPEACFTEDCVRKFKCCQISIEEGKGKLWWNVRKTCYKIVEHNWFETFIVFMILLSSGALAFEDIYIEQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGFQMYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINYTTGEMFDVSVVNNYSECKALIESNQTARWKNVKVNFDNVGLGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEDNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPANKFQGMVFDFVTKQVFDISIMILICLNMVTMMVETDDQSQEMTNILYWINLVFIVLFTGECVLKLISLRYYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGPPDCDPEKDHPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFIEFSKLSDFAAALDPPLLIAKPNKVQLIAMDLPMVSGDRIHCLDILFAFTKRVLGE 1805
BLAST of Sodium channel protein vs. TrEMBL
Match: W5MUS2 (Sodium channel protein OS=Lepisosteus oculatus OX=7918 PE=3 SV=1) HSP 1 Score: 1161.75 bits (3004), Expect = 0.000e+0 Identity = 772/1796 (42.98%), Postives = 1097/1796 (61.08%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFY-NDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCI--AMTKNSE-SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK----VFDNSSIGAETY--ENDSILLWNIKMDYYNNPENW------QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDD--------------------KRFSDLASCQVNTSKAQHDSLILNHQLS-SNKINKVDS---------------KVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVF-----ECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKV------------------------KLYSTYNHI-GKKLMKIIRRHRQIKYPFPWDSNESNP---INIQAAIIKMN---KNETEPSRNVHDPQLNQ-----VAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCC---SESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
LEAGK LP GD + G L+D D +Y N+++TF+V+N K IFRFS+T A ++FSPF+P+R A+I+IL HSLFS F++ TI+ NC+ AM++ ++ ++ VE FT IYT E +IK LARG + FT+LRD WNWLDF VI +AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCV+ + N+S Y +N + W D+ ++ EN+ ++P+ C N + C + C++ +NP++GYTSFD FG + L FRLMTQDYWENLY +R+ G +MI+F++VIF GSFYLIN+IL++VAM+Y++ A +AE ++E E + + +E L E A + + P P + K + R N K H K+L E ++ + + K+ +++ ++ + L + S +R Q ++F KES DD +R + S + S+ +L +N +L+ + N V S KVN + + N + G+ N + ++ +SA SV E E +K RF + + W+CCP + +K+ V F V+DPF++L ITICIVLNT+FM+++ + + +++ NL F IFT E K+IA P YF + WN+FD +IV LS++EL +S +G LS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + KD +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G CI F+ M++GNLV+LNLFLALLLSSFS+++L +D+ ++N LQ A+ R+ R I W+K+ KL NHI G M II R+ + DS +NP IN+ A + + E E S D ++ Q ++ ++ +P + D AE P DC P+ VR CC + + WW +R + ++IV+H +FETFI+ MI+ SS L ED + RK +K ILE+ D FT +F++EM+LKW A G KYF + WCWLDF IV V+LIS D A K++RTLRALRPLRA+SR++GM++VVNALI AIPSI NVLLVCL+FWLIFSI+G+ LF GKF KCV + + S + N+S+C + W ++NFDNV GYL+L Q+ATFKGW +IM AVD + QP +N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QK+K+GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + F+++++ Q FDI+IM I LN + M +E+ Q K + D L+ +N F++IF EC IKI+ALR Y+F + WN+FDF++VI SI V+ + VSPTL RVIR+ RIGRVLRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G++ F+ V GID FNFETFG +MI LFQI+TS GW+S+L ++NN+ EC +K CG+ + + + VSY++ISFL+++NMYIA+ILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F + EPL I +PNKI+L +++P+ G I C+D++ TK LGE
Sbjct: 56 LEAGKQLPRIFGDI--PSELVGVPLDDFDSYYYNNQKTFIVLNKGKAIFRFSATPALYIFSPFHPVRRASIKILVHSLFSLFIMCTILTNCVFMAMSEPAQWAKYVEYTFTGIYTFESLIKILARGFCIGPFTFLRDPWNWLDFSVIVMAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGNLRQKCVQNITDCYHNTSTNGTNYYCKNKT---WPSYQDFISDEENYYKLEGAKDPL-LCGNGSD----AGRCPDGAVCLKI-GKNPDYGYTSFDTFGWAFLALFRLMTQDYWENLYHQTLRSVGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATIAEAWQKEKEFQ-------EAMEQLRKEQQAIVNKVQETDSVLSPEMSPFPM-KDTKERR--NRKKKH--------KTLSEGAEDDAEDKTAKQDTEDRQRGNVFLARTLSSANRRE-----SQCSIFN--FRSRNKESETDFADDECSANGDNASRTGSLVIPWSRRRTSAHSTSSHCSQGNTPTLNINGKLNITVDRNGVSSLGPPSALTLPVVMVDKVNTEDHALQCQNEKTKTCVGVQNGKEPFQRERALSAASVLTNAMEELEESHQKCHPCWYRFAQHYLIWDCCPLWLKIKEWVKFGVMDPFLDLAITICIVLNTLFMALEHHPMTDDFNSMLSVGNLVFTGIFTAEMVFKIIALDPYYYFQQGWNIFDSIIVSLSLMEL----GLSNVEG-LSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYQ--DCVCKISKD-CTLPRWHMKDFFHSFLIVFRVLCGEWIETMWDCMEVAGQPLCILVFMLVMVIGNLVVLNLFLALLLSSFSADNLSAPDDD-GEMNNLQIAIARIQRGINWLKIAICDFFNGNFKKRRQKAKEAKAMTKLQRLGNHIEGNGTMGIIGRNGEKYVISDGDSYMTNPNLTINVPIAPGESDVEFPEEDERSECSEDEEVKQKDEISLSEGSTVDLRKPGEEEDEFSEMAEEAM--DPEDCFPE----MCVRRFPCCDINTTQGVGSIWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDINIEKRKVIKVILEYADKVFTYVFVLEMLLKWVAYGFKKYFTNYWCWLDFLIVDVSLISLIANTLGYSDFAAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGKCVSKAGYQYNASHINNRSECLAMNNMTLYYWTKVKVNFDNVGAGYLALLQVATFKGWMEIMYAAVDSRGLEEQPIKESNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKRKLGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNPFQGFFFDVVSKQAFDIIIMILICLNMVTMMVETDDQSKRMEDILNKINLAFIVIFTGECCIKIMALRCYFFTVGWNIFDFVVVILSIVGIVLADIIEKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMANFAYVKKQGGIDDMFNFETFGNSMICLFQITTSAGWDSLLSPILNNSPEECDANKTHTGTNAKGDCGNPSVGITFFVSYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFEMFYEVWEKFDPEATQFIEYAKLSDFADSLEEPLRIAKPNKIKLISMDLPMVSGDKIHCLDILFAFTKRVLGE 1797
BLAST of Sodium channel protein vs. TrEMBL
Match: A0A1I8JLI5 (Sodium channel protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 1144.03 bits (2958), Expect = 0.000e+0 Identity = 676/1233 (54.83%), Postives = 869/1233 (70.48%), Query Frame = 2
Query: 2024 LSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEP-EMKKRISALSVFECENKLRKRDIYLQRFNKTFCN-WECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYP-------FPWDSNES---NPINIQAA-IIKMNKNETEPSRNVHDPQLNQV----AFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHC----NCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVF-------DAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDS-NNVRLHESLVQNKSDCYEL-QKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK---CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKK 5623
+ S+KI + E+R + ++++ L +NE E K ++ + E + K +K + + +RF C+ W+CC + +K V+FI+LDPF EL+IT+CI++NT FM++D NS ++ NLFF F EA KLIA PR+YF + WN+FDF IVVLS+ EL ++I LS+LR FRLLR+FKLAKSWQTMNLLF II RT+GA+GNL VL II+FIFAVVGMQ+FG Y +KN T +P G PRWNF DFTHSFMIVFRVLCGE+++SMW+CM VNG C+PFFL T IL +LV+L+LFLALLLSSF +ESLQRRE E +VNKLQ L ++ ++ + + + + + + P F + + ++I A+ ++ + NE +R+ N++ F+L LE+ P +P+INE ++++W+ P DC P L +HC + KAWW RC +KIV+HK+FE FI++MI SS+TLT+ED L + +LK +LE MD FTVIF +EMVLKW A GL KYFKDAWCWLDF IV VAL S +F GAFKA+RTLRALRPLRAVSRW+GMK+VVNALIQAIPSIFNVLLVCLVFWLIFSI+G+QLFGG+F+KCVD+ RL +V N+S C E+ +K NVSW NS+INFDNVLNGYL+LFQIAT+KGW I+ADAVD+T V QP+ + +T Y++FVLFIIFG+FFTL LFIGVIIDNFN+QKK+ GGS+E+F+T+ QK+YY AMKKMSS +PQKPVP+PK I+Q F+ I +NQKFD+VIM FIMLNTIVMC+E H Q + + A++ +N+VF++IF ECV+K++A RWYYFK PWNVFDF+IV+ SI W +E + + PT+IRV+RVFR+GRVLRLVKSARGIRTLLFSL VSLPALFNIGLLL++IMFIY+I+G+S FS V +S GID FNFETF M++LFQ+STS GW+SILDGL+N C DKK CGS+ LAVL+LVSYLVISFLVIINMYIAVILENFSQAT+DVQ GLTQ+D+DLFYE WE YDP A GF+ + + EF+ ++ PL +P PN+I+LA L +PICKG TI CVDL+D LT+NFLG + LKPK+ KI ++ VISNT Q++ AAR I LR V ++K
Sbjct: 520 MQSDKIEGGHGHTCQQDSETREQIVDMQEVILLKTLVNESSETAKNQDSIDI-EGKRKEKKTEYFKRRFLDKCCDSWDCCDGWKVFEKYVAFIILDPFTELYITLCILVNTGFMALDMPTNSKKFSEFLSNANLFFTATFAVEAICKLIAMKPRVYFKDGWNIFDFCIVVLSLAELPL-QNIQG----LSILRAFRLLRVFKLAKSWQTMNLLFAIIARTMGALGNLVFVLGIIIFIFAVVGMQLFGENYSTYKNTTLYPHYKGDYPRWNFKDFTHSFMIVFRVLCGEWIDSMWNCMNVNGFICMPFFLLTNILASLVVLSLFLALLLSSFGAESLQRREVEE-EVNKLQGLLIESRDSLCSQELNDEDLFESDSQDVDEPDAENADKRGPRISPDGRFALKRHGAFLMENVSIAASNMVFKDINELHANRDACGEAHNKIYPTCQFSLALEDQFPPAFEEPEINEVDIVYWDAPEDCFPKK----LTKHCPNVMRLFDTTRFGKAWWIFRCQVYKIVEHKYFEMFIILMIALSSITLTVEDVNLHKKPKLKLVLEHMDKCFTVIFTLEMVLKWVAFGLKKYFKDAWCWLDFIIVNVALCSLVLIFLGGASGQSMGAFKAMRTLRALRPLRAVSRWEGMKVVVNALIQAIPSIFNVLLVCLVFWLIFSIMGVQLFGGQFYKCVDNETQERLLPEIVPNQSVCEEMARKFGNVSWQNSRINFDNVLNGYLALFQIATYKGWLGIIADAVDITGVGIQPQRDASTTMYLFFVLFIIFGSFFTLKLFIGVIIDNFNMQKKRAGGSMEIFLTEDQKKYYKAMKKMSSSAPQKPVPKPKLRISQFFFKITSNQKFDVVIMIFIMLNTIVMCIEHHDQSEAFNFAVEQINRVFIVIFTFECVMKLMAQRWYYFKFPWNVFDFVIVVLSILTWAVEDIIQLLRIPPTIIRVVRVFRVGRVLRLVKSARGIRTLLFSLFVSLPALFNIGLLLVMIMFIYSIVGISFFSHVRHSSGIDEMFNFETFPSGMVVLFQLSTSAGWDSILDGLINEKPPHCDPDKKPHSDCGSFALAVLFLVSYLVISFLVIINMYIAVILENFSQATEDVQQGLTQEDFDLFYEKWESYDPKAKGFIPVSRVYEFLDELEPPLQLPMPNRIKLATLNVPICKGETIFCVDLLDALTRNFLGTAADEEQLAPLKPKAGGKIKEERIVISNTLERQKQRHAARIIMNCLRARVEKRK 1741 HSP 2 Score: 399.438 bits (1025), Expect = 1.214e-109 Identity = 219/374 (58.56%), Postives = 270/374 (72.19%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFD-NSSIGAETYEN--------DSILLWNIKMDYYNNPENW-QNP-ITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
+ +L AG LP L + FP KK +G +LED+D FY DK T VVI KTIFRFS+TKA L SPFNP+R AI+ILTH FS VL II NC+ M NS SE VE FTAIYT E IIKA++RGLI+ FTYLRDAWNWLDFIVIGLAY+M+ + ALG LSALRSLRVLRALKTVTT+PG+KT+V +L+ A+ +LRD +LT+F+LSIFAL+GLQ+Y+GTLL KCV F+ + E N IL ++ + NN NW +NP + C + ++ + C E YEC RT+ NP+FGYT+FDNFG S+LCAFRLMTQDYWE+LYQL+IRTNG H+ YF VI GSF+L+NVIL+IVAM+Y
Sbjct: 79 NPKLAAGMKLPPNLSERFPVKKLSGVILEDIDEFYADKLTCVVIARDKTIFRFSATKALMLLSPFNPIRRCAIKILTHPAFSMLVLLAIICNCVFMAMNSSSETVEYTFTAIYTTEAIIKAISRGLILSNFTYLRDAWNWLDFIVIGLAYIMFFSQALGNLSALRSLRVLRALKTVTTVPGLKTIVGALMEAVKRLRDVGILTIFVLSIFALVGLQVYKGTLLQKCVVKFNLTEYLDMEQLTNYTELSSTEQKILEQKLRRLHGNNYSNWYRNPTVRVC---SLSDQVGQQCPEGYECFRTNEANPDFGYTNFDNFGWSMLCAFRLMTQDYWESLYQLVIRTNGKPHVFYFAFVIMLGSFFLMNVILAIVAMAY 449 HSP 3 Score: 86.6557 bits (213), Expect = 7.124e-13 Identity = 58/198 (29.29%), Postives = 102/198 (51.52%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVDSNNVRLHESLVQNKSDCYELQKTEN 4066
IR A KI+ H F +L+ I+ + + + + E ++ FT I+ E ++K + GL Y +DAW WLDF ++ +A I +F G A+R+LR LR L+ V+ G+K +V AL++A+ + +V ++ + IF+++G+Q++ G KCV N+ + + Q ++ EL TE
Sbjct: 146 IRRCAIKILTHPAFSMLVLLAIICNCVFMAMNSS-----------SETVEYTFTAIYTTEAIIKAISRGLILSNFTYLRDAWNWLDFIVIGLAYIMFFSQ-ALGNLSALRSLRVLRALKTVTTVPGLKTIVGALMEAVKRLRDVGILTIFVLSIFALVGLQVYKGTLLQKCVVKFNLTEYLDMEQ-LTNYTELSSTEQ 330 HSP 4 Score: 73.1738 bits (178), Expect = 8.836e-9 Identity = 58/196 (29.59%), Postives = 100/196 (51.02%), Query Frame = 2
Query: 602 RAAAIRILTHSLFSFFVLTTIIINCIAMTKNSES-----------ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYV--------NSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSI 1132
R +I+ H F F++ I ++ I +T + E+++ FT I+T E ++K +A GL Y +DAW WLDFI++ +A V ++G A+R+LR LR L+ V+ GMK VV++L+ AI + + L++ + IF+++G+Q++ G +KCV + E N S+
Sbjct: 1019 RCQVYKIVEHKYFEMFIILMIALSSITLTVEDVNLHKKPKLKLVLEHMDKCFTVIFTLEMVLKWVAFGLK----KYFKDAWCWLDFIIVNVALCSLVLIFLGGASGQSMGAFKAMRTLRALRPLRAVSRWEGMKVVVNALIQAIPSIFNVLLVCLVFWLIFSIMGVQLFGGQ-FYKCVDNETQERLLPEIVPNQSV 1209
BLAST of Sodium channel protein vs. Ensembl Cavefish
Match: scn5lab (sodium channel protein type 4 subunit alpha-like [Source:NCBI gene;Acc:103037861]) HSP 1 Score: 1143.26 bits (2956), Expect = 0.000e+0 Identity = 766/1802 (42.51%), Postives = 1087/1802 (60.32%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFY-NDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINC--IAMTKNSE-SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDN--SSIGAETYENDSILLWNIKMDYYNNPENW---QNPITFCHNF-----------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKL----NVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKES----SKYSLDDKRFSDLA-----------SCQVNTSKAQHDSLILNHQL-SSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINE------PEMKK--RISALSVFEC-ENKLRKRDIYLQR-------FNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYN----------HIGKKLMKIIRRHRQI-----------------KYPFPWDSNESNP-INIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQP------------YDCLPDSIRCF---LVRHCNCC---SESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
LEAGK LP GD G LED+DPFY ++RTF+V+N K IFRFS+T A ++FSPF+P+R A+I+IL HSLFS F++ TI+ NC +AM++ + ++ VE FT IYT E +IK LARG + FT+LRD WNWLDF VI +AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCV+ + ++ Y N S +Y N W + IT NF N+ C + +EC++T NPN+GYTSFD FG + L FRLMTQDYWENLY +R+ G +M++F++VIF GSFYL+N+IL++VAM+Y++ A +AE ++E E ++ +E L E A A + PD + D K H R KSL+ +S+ V++ + + + +L+ + S RT R FN FR +D+ ++ S Y D R L S ++S+ SL +N +L + N + + E N++NE P++ + R ALS C + + + + Q+ F + W C P + LK+ V F+V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF + WN+FD IV LS++EL +S +G LS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ YE + + +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G CI F+ M++GNLV+LNLFLALLLSSFS+++L DE ++N LQ A+ R++R I W++ L +N K ++K+ R +Q K DS +NP + I I + P D + ++ + +P D ++E + +P D PD+ CF VRH CC + + + WW +R + + IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM LKW A G KYF + WCWLDF IV V+L+S D A K++RTLRALRPLRA+SR++GM++VVNALI AIPSI NVLLVCL+FWLIFSI+G+ LF GKF +C++ + S + NK++C + T+ W ++NFDNV GYL+L Q+ATFKGW +IM AVD V QP N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QK+K+GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + F+++++ Q FDI+IM I+LN + M +E+ +Q + L ++N F++IF EC+IK+IALR Y+F + WN+FDF++VI SI V+ + VSPTL RVIR+ RIGR+LRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G++ F+ V GID FNFETFG +MI LFQI+TS GW+S+L ++NN+ EC + + CG+ + + + V+Y++ISFL+++NMYIA+ILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F + EPL I +PNKI+L +++P+ G I C+D++ TK LGE
Sbjct: 55 LEAGKQLPRIFGDI--PSGLVGVPLEDIDPFYFQNQRTFIVLNKGKAIFRFSATSALYIFSPFHPIRRASIKILVHSLFSLFIMCTILTNCCFMAMSEPAPWAKYVEYTFTGIYTFESLIKILARGFCIGPFTFLRDPWNWLDFSVIVMAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGNLRQKCVRSPAHCINNTVPSIYSNSS---------FYCNNRTWLSVHDFITDEDNFYKVEGAKDALICGNSSDAGKCPDGFECMKT-GRNPNYGYTSFDTFGWAFLSLFRLMTQDYWENLYHQTLRSAGKTYMVFFVVVIFLGSFYLVNLILAVVAMAYEEQNQATIAEAWQKEKEFQLA-------MEQLKKEQQAAQK-----AQETESVLVPDLSPSALQD------DKEHKG----RKKSLRPLSEEAENDGVEKTPKLDAIEGVKQTHALLVRTLSS--RTRRGSQVSIFN-FRPRNKDSEADADDEYSVYGDSDSRAGSLVIPWHRRRTSAQSTGSHSSQVFFPSLNINGKLFVAMDQNGISGPGPLSPLPLPTCTMEKVKEESGTNSLNELSSMLLPQLPQEGRDRALSAASCLTDAMEELEEAQQKCHPCWYVFAHQYLVWTCNPGWLKLKEWVKFMVMDPFLDLAITICIVLNTLFMALEHYPMTDEFNKMLSVGNLVFTGIFTAEMVLKIIALDPYYYFQQGWNIFDSFIVCLSLMEL----GLSNVEG-LSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYELCVCKI---SKDCILPRWHMKDFFHSFLIVFRVLCGEWIETMWDCMEVAGQPLCILVFMLVMVIGNLVVLNLFLALLLSSFSADNLSA-PDEDGEMNNLQIAIARIHRGIGWLRRALCDFFNGNFKRRRQKAKEAKAMLKLKRLSQQAQRAEGNGTAGVIARTGDKCAATDDSYMTNPNLTISVPIAPGESDVEFPEEEDDDDEASESSDEEEEAETKPS--DDISLSEGSTVDLRKPGEEEDEYSEMAEEDMDPDN--CFPEVCVRHFQCCDINTNEGLGRVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYIEQRKVVKIVLEYADKIFTYIFILEMTLKWIAYGFKKYFTNYWCWLDFLIVDVSLVSLVANTLGYSDFAAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCLNRTGFVYNASHINNKTECLAMNDTQ-YYWSKVKVNFDNVGAGYLALLQVATFKGWMEIMYAAVDSRAVEEQPVKEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKRKLGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNPLQGFFFDLVSKQAFDIMIMLLILLNMVTMMVETDEQSPRMEMILYNINLAFIVIFTTECIIKLIALRCYFFTVSWNIFDFVVVILSIVGIVLADIIEKYFVSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMANFAYVKKQSGIDDMFNFETFGNSMICLFQITTSAGWDSLLGPILNNSPEECDPNIPHTGTNARGNCGNPSVGITFFVTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFEMFYEVWEKFDPEATQFIEYAKLSDFADTLSEPLRIAKPNKIKLISMDLPMVSGDKIHCLDILFAFTKRVLGE 1802
BLAST of Sodium channel protein vs. Ensembl Cavefish
Match: scn5lab (sodium channel protein type 4 subunit alpha-like [Source:NCBI gene;Acc:103037861]) HSP 1 Score: 1140.56 bits (2949), Expect = 0.000e+0 Identity = 763/1801 (42.37%), Postives = 1083/1801 (60.13%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFY-NDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINC--IAMTKNSE-SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDN--SSIGAETYENDSILLWNIKMDYYNNPENW---QNPITFCHNF-----------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKL----NVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKES----SKYSLDDKRFSDLA-----------SCQVNTSKAQHDSLILNHQL-SSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINE------PEMKK--RISALSVFEC-ENKLRKRDIYLQR-------FNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYN----------HIGKKLMKIIRRHRQI-----------------KYPFPWDSNESNP-INIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQP------------YDCLPDSIRCF---LVRHCNCC---SESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
LEAGK LP GD G LED+DPFY ++RTF+V+N K IFRFS+T A ++FSPF+P+R A+I+IL HSLFS F++ TI+ NC +AM++ + ++ VE FT IYT E +IK LARG + FT+LRD WNWLDF VI +A++ +G L ALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCV+ + ++ Y N S +Y N W + IT NF N+ C + +EC++T NPN+GYTSFD FG + L FRLMTQDYWENLY +R+ G +M++F++VIF GSFYL+N+IL++VAM+Y++ A +AE ++E E ++ +E L E A A + PD + D K H R KSL+ +S+ V++ + + + +L+ + S RT R FN FR +D+ ++ S Y D R L S ++S+ SL +N +L + N + + E N++NE P++ + R ALS C + + + + Q+ F + W C P + LK+ V F+V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF + WN+FD IV LS++EL +S +G LS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ YE + + +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G CI F+ M++GNLV+LNLFLALLLSSFS+++L DE ++N LQ A+ R++R I W++ L +N K ++K+ R +Q K DS +NP + I I + P D + ++ + +P D ++E + +P D PD+ CF VRH CC + + + WW +R + + IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM LKW A G KYF + WCWLDF IV V+L+S D A K++RTLRALRPLRA+SR++GM++VVNALI AIPSI NVLLVCL+FWLIFSI+G+ LF GKF +C++ + S + NK++C + T+ W ++NFDNV GYL+L Q+ATFKGW +IM AVD V QP N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QK+K+GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + F+++++ Q FDI+IM I+LN + M +E+ +Q + L ++N F++IF EC+IK+IALR Y+F + WN+FDF++VI SI V+ + VSPTL RVIR+ RIGR+LRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G++ F+ V GID FNFETFG +MI LFQI+TS GW+S+L ++NN+ EC + + CG+ + + + V+Y++ISFL+++NMYIA+ILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F + EPL I +PNKI+L +++P+ G I C+D++ TK LGE
Sbjct: 55 LEAGKQLPRIFGDI--PSGLVGVPLEDIDPFYFQNQRTFIVLNKGKAIFRFSATSALYIFSPFHPIRRASIKILVHSLFSLFIMCTILTNCCFMAMSEPAPWAKYVEYTFTGIYTFESLIKILARGFCIGPFTFLRDPWNWLDFSVIVMAFLTEF-IKVGNLQALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGNLRQKCVRSPAHCINNTVPSIYSNSS---------FYCNNRTWLSVHDFITDEDNFYKVEGAKDALICGNSSDAGKCPDGFECMKT-GRNPNYGYTSFDTFGWAFLSLFRLMTQDYWENLYHQTLRSAGKTYMVFFVVVIFLGSFYLVNLILAVVAMAYEEQNQATIAEAWQKEKEFQLA-------MEQLKKEQQAAQK-----AQETESVLVPDLSPSALQD------DKEHKG----RKKSLRPLSEEAENDGVEKTPKLDAIEGVKQTHALLVRTLSS--RTRRGSQVSIFN-FRPRNKDSEADADDEYSVYGDSDSRAGSLVIPWHRRRTSAQSTGSHSSQVFFPSLNINGKLFVAMDQNGISGPGPLSPLPLPTCTMEKVKEESGTNSLNELSSMLLPQLPQEGRDRALSAASCLTDAMEELEEAQQKCHPCWYVFAHQYLVWTCNPGWLKLKEWVKFMVMDPFLDLAITICIVLNTLFMALEHYPMTDEFNKMLSVGNLVFTGIFTAEMVLKIIALDPYYYFQQGWNIFDSFIVCLSLMEL----GLSNVEG-LSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYELCVCKI---SKDCILPRWHMKDFFHSFLIVFRVLCGEWIETMWDCMEVAGQPLCILVFMLVMVIGNLVVLNLFLALLLSSFSADNLSA-PDEDGEMNNLQIAIARIHRGIGWLRRALCDFFNGNFKRRRQKAKEAKAMLKLKRLSQQAQRAEGNGTAGVIARTGDKCAATDDSYMTNPNLTISVPIAPGESDVEFPEEEDDDDEASESSDEEEEAETKPS--DDISLSEGSTVDLRKPGEEEDEYSEMAEEDMDPDN--CFPEVCVRHFQCCDINTNEGLGRVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYIEQRKVVKIVLEYADKIFTYIFILEMTLKWIAYGFKKYFTNYWCWLDFLIVDVSLVSLVANTLGYSDFAAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCLNRTGFVYNASHINNKTECLAMNDTQ-YYWSKVKVNFDNVGAGYLALLQVATFKGWMEIMYAAVDSRAVEEQPVKEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKRKLGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNPLQGFFFDLVSKQAFDIMIMLLILLNMVTMMVETDEQSPRMEMILYNINLAFIVIFTTECIIKLIALRCYFFTVSWNIFDFVVVILSIVGIVLADIIEKYFVSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMANFAYVKKQSGIDDMFNFETFGNSMICLFQITTSAGWDSLLGPILNNSPEECDPNIPHTGTNARGNCGNPSVGITFFVTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFEMFYEVWEKFDPEATQFIEYAKLSDFADTLSEPLRIAKPNKIKLISMDLPMVSGDKIHCLDILFAFTKRVLGE 1802
BLAST of Sodium channel protein vs. Ensembl Cavefish
Match: scn5lab (sodium channel protein type 4 subunit alpha-like [Source:NCBI gene;Acc:103037861]) HSP 1 Score: 1140.18 bits (2948), Expect = 0.000e+0 Identity = 767/1802 (42.56%), Postives = 1084/1802 (60.16%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFY-NDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINC--IAMTKNSE-SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDN--SSIGAETYENDSILLWNIKMDYYNNPENW---QNPITFCHNF-----------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKL----NVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRK----ESSKYSLDDKRFSDLA-----------SCQVNTSKAQHDSLILNHQL-SSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINE------PEMKK--RISALSVFEC-ENKLRKRDIYLQR-------FNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYN----------HIGKKLMKIIRRHRQI-----------------KYPFPWDSNESNP-INIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQP------------YDCLPDSIRCF---LVRHCNCC---SESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFK---VFDA-GAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
LEAGK LP GD G LED+DPFY ++RTF+V+N K IFRFS+T A ++FSPF+P+R A+I+IL HSLFS F++ TI+ NC +AM++ + ++ VE FT IYT E +IK LARG + FT+LRD WNWLDF VI +AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCV+ + ++ Y N S +Y N W + IT NF N+ C + +EC++T NPN+GYTSFD FG + L FRLMTQDYWENLY +R+ G +M++F++VIF GSFYL+N+IL++VAM+Y++ A +AE ++E E ++ +E L E A A + PD + D K H R KSL+ +S+ V++ + + + +L+ + S RT R FN FR +D+ E S Y D R L S ++S+ SL +N +L + N + + E N++NE P++ + R ALS C + + + + Q+ F + W C P + LK+ V F+V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF + WN+FD IV LS++EL +S +G LS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ YE + + +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G CI F+ M++GNLV+LNLFLALLLSSFS+++L DE ++N LQ A+ R++R I W++ L +N K ++K+ R +Q K DS +NP + I I + P D + ++ + +P D ++E + +P D PD+ CF VRH CC + + + WW +R + + IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM LKW A G KYF + WCWLDF IV V++I FD G + +RTLRALRPLRAVSR+ GM++VVNALI AIPSI NVLLVCL+FWLIFSI+G+ LF GKF +C++ + S + NK++C + T+ W ++NFDNV GYL+L Q+ATFKGW +IM AVD V QP N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QK+K+GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + F+++++ Q FDI+IM I+LN + M +E+ +Q + L ++N F++IF EC+IK+IALR Y+F + WN+FDF++VI SI V+ + VSPTL RVIR+ RIGR+LRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G++ F+ V GID FNFETFG +MI LFQI+TS GW+S+L ++NN+ EC + + CG+ + + + V+Y++ISFL+++NMYIA+ILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F + EPL I +PNKI+L +++P+ G I C+D++ TK LGE
Sbjct: 55 LEAGKQLPRIFGDI--PSGLVGVPLEDIDPFYFQNQRTFIVLNKGKAIFRFSATSALYIFSPFHPIRRASIKILVHSLFSLFIMCTILTNCCFMAMSEPAPWAKYVEYTFTGIYTFESLIKILARGFCIGPFTFLRDPWNWLDFSVIVMAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGNLRQKCVRSPAHCINNTVPSIYSNSS---------FYCNNRTWLSVHDFITDEDNFYKVEGAKDALICGNSSDAGKCPDGFECMKT-GRNPNYGYTSFDTFGWAFLSLFRLMTQDYWENLYHQTLRSAGKTYMVFFVVVIFLGSFYLVNLILAVVAMAYEEQNQATIAEAWQKEKEFQLA-------MEQLKKEQQAAQK-----AQETESVLVPDLSPSALQD------DKEHKG----RKKSLRPLSEEAENDGVEKTPKLDAIEGVKQTHALLVRTLSS--RTRRGSQVSIFN-FRPRNKDSEADADDEYSVYGDSDSRAGSLVIPWHRRRTSAQSTGSHSSQVFFPSLNINGKLFVAMDQNGISGPGPLSPLPLPTCTMEKVKEESGTNSLNELSSMLLPQLPQEGRDRALSAASCLTDAMEELEEAQQKCHPCWYVFAHQYLVWTCNPGWLKLKEWVKFMVMDPFLDLAITICIVLNTLFMALEHYPMTDEFNKMLSVGNLVFTGIFTAEMVLKIIALDPYYYFQQGWNIFDSFIVCLSLMEL----GLSNVEG-LSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYELCVCKI---SKDCILPRWHMKDFFHSFLIVFRVLCGEWIETMWDCMEVAGQPLCILVFMLVMVIGNLVVLNLFLALLLSSFSADNLSA-PDEDGEMNNLQIAIARIHRGIGWLRRALCDFFNGNFKRRRQKAKEAKAMLKLKRLSQQAQRAEGNGTAGVIARTGDKCAATDDSYMTNPNLTISVPIAPGESDVEFPEEEDDDDEASESSDEEEEAETKPS--DDISLSEGSTVDLRKPGEEEDEYSEMAEEDMDPDN--CFPEVCVRHFQCCDINTNEGLGRVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYIEQRKVVKIVLEYADKIFTYIFILEMTLKWIAYGFKKYFTNYWCWLDFLIVDVSVIGLLARALSFDQFGTIRVLRTLRALRPLRAVSRFAGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCLNRTGFVYNASHINNKTECLAMNDTQ-YYWSKVKVNFDNVGAGYLALLQVATFKGWMEIMYAAVDSRAVEEQPVKEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKRKLGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNPLQGFFFDLVSKQAFDIMIMLLILLNMVTMMVETDEQSPRMEMILYNINLAFIVIFTTECIIKLIALRCYFFTVSWNIFDFVVVILSIVGIVLADIIEKYFVSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMANFAYVKKQSGIDDMFNFETFGNSMICLFQITTSAGWDSLLGPILNNSPEECDPNIPHTGTNARGNCGNPSVGITFFVTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFEMFYEVWEKFDPEATQFIEYAKLSDFADTLSEPLRIAKPNKIKLISMDLPMVSGDKIHCLDILFAFTKRVLGE 1802
BLAST of Sodium channel protein vs. Ensembl Cavefish
Match: scn5lab (sodium channel protein type 4 subunit alpha-like [Source:NCBI gene;Acc:103037861]) HSP 1 Score: 1088.56 bits (2814), Expect = 0.000e+0 Identity = 746/1798 (41.49%), Postives = 1057/1798 (58.79%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFY-NDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINC--IAMTKNSE-SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDN--SSIGAETYENDSILLWNIKMDYYNNPENW---QNPITFCHNF-----------TSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKL----NVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRK----ESSKYSLDDKRFSDLA-----------SCQVNTSKAQHDSLILNHQL-SSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINE------PEMKK--RISALSVFEC-ENKLRKRDIYLQR-------FNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYN----------HIGKKLMKIIRRHRQI-----------------KYPFPWDSNESNP-INIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQP------------YDCLPDSIRCF---LVRHCNCC---SESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
LEAGK LP GD G LED+DPFY ++RTF+V+N K IFRFS+T A ++FSPF+P+R A+I+IL HSLFS F++ TI+ NC +AM++ + ++ VE FT IYT E +IK LARG + FT+LRD WNWLDF VI +AY+ +V+ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCV+ + ++ Y N S +Y N W + IT NF N+ C + +EC++T NPN+GYTSFD FG + L FRLMTQDYWENLY +R+ G +M++F++VIF GSFYL+N+IL++VAM+Y++ A +AE ++E E ++ +E L E A A + PD + D K H R KSL+ +S+ V++ + + + +L+ + S RT R FN FR +D+ E S Y D R L S ++S+ SL +N +L + N + + E N++NE P++ + R ALS C + + + + Q+ F + W C P + LK+ V F+V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +K+IA P YF + WN+FD IV LS++EL +S +G LS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ YE + + +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G CI F+ M++GNLV+LNLFLALLLSSFS+++L DE ++N LQ A+ R++R I W++ L +N K ++K+ R +Q K DS +NP + I I + P D + ++ + +P D ++E + +P D PD+ CF VRH CC + + + WW +R + + IV+H +FETFI+ MI+ SS L ED ++ RK +K +LE+ D FT IF++EM LKW A G KYF + WCWLDF IV +VVNALI AIPSI NVLLVCL+FWLIFSI+G+ LF GKF +C++ + S + NK++C + T+ W ++NFDNV GYL+L Q+ATFKGW +IM AVD V QP N + Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QK+K+GG ++FMT+ QK+YY+AMKK+ S PQKP+PRP + + F+++++ Q FDI+IM I+LN + M +E+ +Q + L ++N F++IF EC+IK+IALR Y+F + WN+FDF++VI SI V+ + VSPTL RVIR+ RIGR+LRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G++ F+ V GID FNFETFG +MI LFQI+TS GW+S+L ++NN+ EC + + CG+ + + + V+Y++ISFL+++NMYIA+ILENFS AT++ L++DD+++FYE WE++DP AT F+E LS+F + EPL I +PNKI+L +++P+ G I C+D++ TK LGE
Sbjct: 55 LEAGKQLPRIFGDI--PSGLVGVPLEDIDPFYFQNQRTFIVLNKGKAIFRFSATSALYIFSPFHPIRRASIKILVHSLFSLFIMCTILTNCCFMAMSEPAPWAKYVEYTFTGIYTFESLIKILARGFCIGPFTFLRDPWNWLDFSVIVMAYVTEFVD--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGNLRQKCVRSPAHCINNTVPSIYSNSS---------FYCNNRTWLSVHDFITDEDNFYKVEGAKDALICGNSSDAGKCPDGFECMKT-GRNPNYGYTSFDTFGWAFLSLFRLMTQDYWENLYHQTLRSAGKTYMVFFVVVIFLGSFYLVNLILAVVAMAYEEQNQATIAEAWQKEKEFQLA-------MEQLKKEQQAAQK-----AQETESVLVPDLSPSALQD------DKEHKG----RKKSLRPLSEEAENDGVEKTPKLDAIEGVKQTHALLVRTLSS--RTRRGSQVSIFN-FRPRNKDSEADADDEYSVYGDSDSRAGSLVIPWHRRRTSAQSTGSHSSQVFFPSLNINGKLFVAMDQNGISGPGPLSPLPLPTCTMEKVKEESGTNSLNELSSMLLPQLPQEGRDRALSAASCLTDAMEELEEAQQKCHPCWYVFAHQYLVWTCNPGWLKLKEWVKFMVMDPFLDLAITICIVLNTLFMALEHYPMTDEFNKMLSVGNLVFTGIFTAEMVLKIIALDPYYYFQQGWNIFDSFIVCLSLMEL----GLSNVEG-LSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYELCVCKI---SKDCILPRWHMKDFFHSFLIVFRVLCGEWIETMWDCMEVAGQPLCILVFMLVMVIGNLVVLNLFLALLLSSFSADNLSA-PDEDGEMNNLQIAIARIHRGIGWLRRALCDFFNGNFKRRRQKAKEAKAMLKLKRLSQQAQRAEGNGTAGVIARTGDKCAATDDSYMTNPNLTISVPIAPGESDVEFPEEEDDDDEASESSDEEEEAETKPS--DDISLSEGSTVDLRKPGEEEDEYSEMAEEDMDPDN--CFPEVCVRHFQCCDINTNEGLGRVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYIEQRKVVKIVLEYADKIFTYIFILEMTLKWIAYGFKKYFTNYWCWLDFLIV-------------------------------------DVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCLNRTGFVYNASHINNKTECLAMNDTQ-YYWSKVKVNFDNVGAGYLALLQVATFKGWMEIMYAAVDSRAVEEQPVKEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKRKLGGQ-DIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNPLQGFFFDLVSKQAFDIMIMLLILLNMVTMMVETDEQSPRMEMILYNINLAFIVIFTTECIIKLIALRCYFFTVSWNIFDFVVVILSIVGIVLADIIEKYFVSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMANFAYVKKQSGIDDMFNFETFGNSMICLFQITTSAGWDSLLGPILNNSPEECDPNIPHTGTNARGNCGNPSVGITFFVTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFEMFYEVWEKFDPEATQFIEYAKLSDFADTLSEPLRIAKPNKIKLISMDLPMVSGDKIHCLDILFAFTKRVLGE 1761
BLAST of Sodium channel protein vs. Ensembl Cavefish
Match: scn1laa (sodium channel, voltage-gated, type I-like, alpha [Source:ZFIN;Acc:ZDB-GENE-040426-751]) HSP 1 Score: 873.618 bits (2256), Expect = 0.000e+0 Identity = 554/1249 (44.36%), Postives = 774/1249 (61.97%), Query Frame = 2
Query: 1958 DLASCQVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKV----------------------------KLYSTYNHIGKKLMK------------------IIRRHRQIKYPFPWDSNESNPINIQ-----AAIIKMNKNETE--PSRNV----------HDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD-SNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
D A+ N KAQ+DS HQ S +++ ++ +R +++I+ +E E E +K RF TF WECCP + +KK V +V+DPF++L ITICIV+NT+FM+++ + +++ N F IFT E K+IA P YF E WN+FD +IV LS++EL A+ +S+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+ NL +VLAII+FIFAVVGMQ+FG+ Y+ + T+ D RW+ DF HSF+IVFRVLCGE++E+MWDCM V+G C+ FL M++GNLV+LNLFLALLLSSFS+++L ED++ ++N LQ AL R++R + +VK KL NH +L+K II + P +++ N++ ++ ++ +K PSRNV +L++V + E + D D E E+ P C D VR C CC E + WW +R + F+IV+H +FE+FI+ MI+ SS L ED ++ K LK ILE+ D FT +F++EM+LKW A G KYF +AWCWLDF IV V+LIS + GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GKF++CV+ + +R++E+ V NK++C ++ N W N +INFDNV GYL+L Q+ATFKGW DIM AVD +N QPK+ N Y YFV FIIFG+FFTLNLFIGVIIDNFN QKKKI S ++FMT+ QK+YY+AMKK+ S QKP+PRP + + ++I+ NQ FDI IM I LN + M +ES ++ L+ +N +FV +F EC +K+++LR Y+F + WN+FDF++VI SI I + + SPTL+RV+R+ RIGR+LRL+K A+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N + +C K CGS + +++ VSY++I FL+++NMYIAVILENFS AT++ L++DD+++FYE WE++DP AT FME LS+F + PL +P+PNKI+L +++P+ G I C+D++ TK LGE
Sbjct: 576 DKATTDENVRKAQYDS----HQPSPGYLDEQRAR----QRALSVASIITNTME---------------------ELEESRQKCHPCWYRFAHTFLIWECCPTWMKIKKAVHMVVMDPFVDLSITICIVMNTIFMAMEHHPMDKDFSSVLSMGNYVFTGIFTAEMVFKIIALDPYYYFQEGWNIFDSVIVSLSLMELGL-----ANISGMSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSMGALSNLTLVLAIIIFIFAVVGMQLFGKSYK--TSLTKIAPDG--FLRWHMMDFLHSFLIVFRVLCGEWIETMWDCMEVHGQPLCLIVFLMVMVIGNLVVLNLFLALLLSSFSADNLAATEDDN-EMNNLQIALGRIHRGVAFVKASLRRFFQTVCLRVRRKKSSEQNGLKNSKKLPDISNHTTVELIKDPGSMKDLAGADSDNENFIINSSVTVTVPIATADSDAECQNMEDYSSYSSDMEEDKERRHCVPSRNVVMEMCAVSGLFLQKLSEVMSSSEGSTVEIRKDG-ADSLEFELEESMDPEPCFTDGC----VRRCRCCQVNMEKERWRTWWTVRRTCFRIVEHTWFESFIIFMILLSSGALAFEDIYIEGYKTLKMILEYADKVFTYVFMLEMLLKWLAYGFAKYFTNAWCWLDFLIVDVSLISLVANALDYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYECVNITTGIRMNETDVANKTEC-DMLGEGNARWKNLKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRNLNEQPKFEVNLIMYGYFVGFIIFGSFFTLNLFIGVIIDNFNQQKKKI--SQDIFMTEEQKKYYNAMKKLGSKKLQKPIPRPTNKVQGFIFDIVTNQAFDIFIMILICLNMVTMMVESDDPDPKMKTVLNWLNMIFVALFTTECALKMVSLRQYFFTVGWNIFDFVVVILSIVAMCISDVITY-FFSPTLLRVVRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKKEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLSPILNRDIPDCNPTKDNPGSKVKGDCGSPSIGIIFFVSYIIICFLIVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPKATQFMEYNKLSDFADALDPPLRMPKPNKIQLIGMDLPMVTGERIHCLDILFAFTKRVLGE 1776 HSP 2 Score: 226.098 bits (575), Expect = 8.604e-59 Identity = 157/381 (41.21%), Postives = 223/381 (58.53%), Query Frame = 2
Query: 401 KRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRIL---THSL--FSFFVLTTIIINCIAMTKNSESEN----VETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSI---GAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTS-------NNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
+ LEAGK LP GD + LED+DPFY +++TF+V+N KTIFRF++T A F+ S FNPLR +I+IL T ++ F+ ++ TI+ NC MT SES E FT IYT E +IK LARG + +FT+LRD WNWLDF VI LAY+ +G +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L +KC+++ N + G+ +Y ++ PE+W N+TS N +K+ C+ CI + SFD+ C L +R G +MI+F++VIF GSFYL+N+IL++VAM+Y
Sbjct: 54 RDLEAGKKLPLIYGDI--PQGLVSTPLEDMDPFYCNQKTFIVLNRGKTIFRFNATPALFILSSFNPLRRISIKILFSLTEAVTWFNMVIMFTILTNCAFMTM-SESPGWAKIAEYTFTGIYTLESVIKILARGFCIGKFTFLRDPWNWLDFSVIVLAYVTEF-VKMGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLKYKCIQLPANHTDLGNGSISYNGTAV------------PEDW------LKNYTSDPCECWFNCKLKTCCSFFLFCI--------LLFKSFDH-----KC---------------LTLRAAGKTYMIFFVLVIFLGSFYLVNLILAVVAMAY 384 HSP 3 Score: 82.8037 bits (203), Expect = 4.047e-15 Identity = 43/120 (35.83%), Postives = 74/120 (61.67%), Query Frame = 2
Query: 3644 FTVIFLVEMVLK----WFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF-FKCV 3988
FT I+ +E V+K F IG + +D W WLDF+++++A ++ F G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G +KC+
Sbjct: 167 FTGIYTLESVIKILARGFCIGKFTFLRDPWNWLDFSVIVLAYVTEF--VKMGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGHLKYKCI 284 HSP 4 Score: 65.0846 bits (157), Expect = 8.161e-10 Identity = 78/318 (24.53%), Postives = 140/318 (44.03%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSA-----LGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNG-------DVHMI---YFMMVIFFGSFYLINVILSIV 1474
+R RI+ H+ F F++ I+++ A+ T E + +FT ++ E ++K LA G Y +AW WLDF+++ ++ + V +A LG + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G ++CV N + G E D N E C N R N N FDN G L ++ T W ++ + + +V++I YF+ I FGSF+ +N+ + ++
Sbjct: 1109 VRRTCFRIVEHTWFESFIIFMILLSSGALAFEDIYIEGYKTLKMILEYADKVFTYVFMLEMLLKWLAYGFA----KYFTNAWCWLDFLIVDVSLISLVANALDYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK-FYECV----NITTGIRMNET----------DVANKTE--------CDMLGEGNA------------RWKNLKIN-----FDNVGAGYLALLQVATFKGWMDIMYAAVDSRNLNEQPKFEVNLIMYGYFVGFIIFGSFFTLNLFIGVI 1382
BLAST of Sodium channel protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008004.1 (pep scaffold:Pmarinus_7.0:GL477228:88466:118438:1 gene:ENSPMAG00000007222.1 transcript:ENSPMAT00000008004.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 511.531 bits (1316), Expect = 8.961e-152 Identity = 286/728 (39.29%), Postives = 455/728 (62.50%), Query Frame = 2
Query: 3446 NCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFK--VFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLXXXXXXXXXXX------XFVISNTFLYQRELWAARKITGFLRE 5605
CC C+ A+ +R F++ F +L M++ SS TLTL+DK+ + +L +L +MD+ + F +EM LK +GL KYF W LD +VLV+ ++ V + +++RTLRALRPLRA+SRW GM++VVNAL+ +IPSI NV+L+CL+FW++F I G+QLFGGKFF+C+D+ RL + V N++ C L E WVN+ IN+DN+L+ YL+L Q+AT +GW + MADA D T V+ QP YN N + ++F++F+I G+FF LNLFIGV+IDNFN K+ G+L +T+ Q+++Y M+++ P K +P+P++ + + FY +++ + F+ + I LN +++ E +KQ + ++ +N VF +F LE +KI+ R +YF+ PW++FDF +V S+ V+E SP ++RV RV R+ R+LRL++S GIR L+++LV+SLPAL NIGLLL+LIMF+Y ++GVS+F ++ YS I NFETF +M +LF+++T+GGWN ++ ++ T IL + Y ++++ S ++IINMY+A+ILENF++A + + G+T DD D+FY W ++DP AT F+ + L F + P IP+PN +++A L++ I +G+ + C+D++ +T+ G + L +++ K+ + V+S T +RE AA I R
Sbjct: 998 GCCG-GCV-GAFHAVRRMVFRLCHTTGFNGLVLFMVIMSSFTLTLDDKYNSSNAQLMQVLRYMDIFYMAFFSMEMFLKMIGLGLHKYFTSFWTLLDCFLVLVSWLNQLASAVPALKSLRSLRTLRALRPLRAISRWKGMRLVVNALVTSIPSISNVMLMCLLFWMVFGIAGVQLFGGKFFRCIDAEGNRLPATEVANRTVCESL-AAEGYLWVNAIINYDNILSAYLALLQVATLEGWIEQMADASDSTGVDQQPSYNANIYSQMFFIIFVIIGSFFILNLFIGVVIDNFNSVHKR-EGALMALLTEDQQKFYKTMRRLYRSKPSKSIPQPQNRVLRFFYLLVHRRLFEYISTAVISLNMVMLASEHYKQSESFTNITKIINLVFTGLFMLEAALKILGNRIHYFRQPWDIFDFFVVCISLTEIVLETMASQVNFSPAILRVFRVVRLVRLLRLIRSVEGIRKLIYTLVISLPALINIGLLLLLIMFMYGVLGVSIFRDLPYSGSITNMVNFETFANSMTLLFRLNTAGGWNDVMGSVLGTKTQNA----------ILVIAYFTTFVIFSNIIIINMYVAIILENFNEAQEQEETGVTDDDIDMFYRVWGKFDPLATQFLACEQLPNFFDALKRPFRIPKPNTVKIAALQLAIVEGNKVHCLDVLKTMTRVIFGRVVKTEHLRQLSRQAQEKVMRRFPLRRIMHVVSTTLALRREEKAAHVIQRAFRH 1711 HSP 2 Score: 183.726 bits (465), Expect = 1.302e-46 Identity = 106/272 (38.97%), Postives = 164/272 (60.29%), Query Frame = 2
Query: 2138 EPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGI-YCIPFFLAT 2950
P + + S+ R R+ R + CC A+ ++ + +V E FITI I+ NTV M+ID Y+ S ++ N IF EA +KL A PR+YF WN+FDF +V+ S+VE +S + N+S++RTFR+LR+FKLAK W+T+NLL ++ +G + NL ++ A++++IFAV+GMQ+ GR Y + N+ +F +G++PRWNF DF SF++VFRVLCGE++E +++C+ + I YCIP F+ T
Sbjct: 516 RPPIASHVPPCSLLSVRAARRPRE----RLVACLNSLRCCSAFVAFRQRLRTLVTSSVFETFITILIITNTVMMAIDYYNAPYSSA--LDIGNTVLTTIFIAEAVLKLTALFPRVYFHLGWNIFDFCVVITSVVEWILD-SMSNTNINISIIRTFRILRVFKLAKKWKTLNLLIRVMKSAIGEMRNLTVIFALVIYIFAVIGMQLIGRDY--YHNRNKF--SSGQVPRWNFLDFYSSFLMVFRVLCGEWIEPLYECLVCSNIYYCIPLFILT 776 HSP 3 Score: 170.244 bits (430), Expect = 1.744e-42 Identity = 101/247 (40.89%), Postives = 144/247 (58.30%), Query Frame = 2
Query: 719 IFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENW----QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFY 1447
IFTAIYT EF+IK LARG + + ++YLR+AWNWLDFIVI LAYM A L L +LR RALK + +P +KT+V ++ + L + ++L +F L I +L GLQ++RG L KCV + + + E W+ +Y N NW I C N T S C Y C+ ENPN G TSFDNFG +++ +F+++T DYWEN Y +++R +G + +F++VI G +Y
Sbjct: 2 IFTAIYTGEFLIKTLARGFVFHPYSYLRNAWNWLDFIVIILAYMTI---AFPFLPGLSALRAFRALKMIAILPALKTIVGAIFKSAGLLLNVMLLMIFALIIISLFGLQIFRGVLRQKCVFLPNPGTSSLSDAE------WS---NYVQNKSNWLITDDIEIMVCGNGTGT----SHCPANYTCLPDIGENPNHGATSFDNFGWAMMTSFQIITIDYWENCYNMVLRASGQYFIGFFIVVIMLGGYY 232 HSP 4 Score: 68.1662 bits (165), Expect = 2.247e-11 Identity = 47/175 (26.86%), Postives = 85/175 (48.57%), Query Frame = 2
Query: 3494 CSAF--------KIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGA-----FKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF 3979
CSAF +V FETFI I+I+ +++ + ++ P L+ + T IF+ E VLK A+ YF W DF +V+ +++ + + D+ + IRT R LR + +W + +++ + AI + N+ ++ + IF++IGMQL G ++
Sbjct: 551 CSAFVAFRQRLRTLVTSSVFETFITILIITNTVMMAIDYYNAP----YSSALDIGNTVLTTIFIAEAVLKLTALFPRVYFHLGWNIFDFCVVITSVVEW--ILDSMSNTNINISIIRTFRILRVFKLAKKWKTLNLLIRVMKSAIGEMRNLTVIFALVIYIFAVIGMQLIGRDYY 719 HSP 5 Score: 62.003 bits (149), Expect = 1.463e-9 Identity = 62/218 (28.44%), Postives = 111/218 (50.92%), Query Frame = 2
Query: 4448 QIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIAL-RWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAY------SYGIDATFNFETFGKAMIILFQISTSGGW-NSILDGLMNNNTYEC 5077
Q ++ + F+ I I+ NT++M ++ + S ALD N V IF E V+K+ AL YF + WN+FDF +VI S+ W+++ N I +IR FRI RV +L K + + L+ + ++ + N+ ++ L+++I+A+IG+ + Y S G +NF F + +++F++ G W + + L+ +N Y C
Sbjct: 559 QRLRTLVTSSVFETFITILIITNTVMMAIDYYN--APYSSALDIGNTVLTTIFIAEAVLKLTALFPRVYFHLGWNIFDFCVVITSVVEWILDSMSNTN----INISIIRTFRILRVFKLAKKWKTLNLLIRVMKSAIGEMRNLTVIFALVIYIFAVIGMQLIGRDYYHNRNKFSSGQVPRWNFLDFYSSFLMVFRV-LCGEWIEPLYECLVCSNIYYC 769 HSP 6 Score: 61.6178 bits (148), Expect = 1.998e-9 Identity = 43/144 (29.86%), Postives = 75/144 (52.08%), Query Frame = 2
Query: 3644 FTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV---DSNNVRLHES----LVQNKSD 4039
FT I+ E ++K A G Y ++AW WLDF ++++A ++ F G + LRA R L+ ++ +K +V A+ ++ + NV+L+ + +I S+ G+Q+F G KCV + L ++ VQNKS+
Sbjct: 3 FTAIYTGEFLIKTLARGFVFHPYSYLRNAWNWLDFIVIILAYMTIAFPFLPG----LSALRAFRALKMIAILPALKTIVGAIFKSAGLLLNVMLLMIFALIIISLFGLQIFRGVLRQKCVFLPNPGTSSLSDAEWSNYVQNKSN 142 HSP 7 Score: 61.6178 bits (148), Expect = 2.287e-9 Identity = 47/215 (21.86%), Postives = 104/215 (48.37%), Query Frame = 2
Query: 2183 CENKLRKRDIYLQR----------------FNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNS--VSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYER 2773
C ++ R+RD ++R + C C A+ ++++V + + +++++ +++D NS ++ ++ +++F+M F+ E +K+I G YF+ W + D +V++S + AS + A K +L LRT R LR + W+ M L+ + ++ +I N+ ++ + +F + G+Q+FG ++ R
Sbjct: 964 CCHRQRRRDSDVERAVGSGGGDGEGGGRHGEEPSGCCGGCVGAFHAVRRMVFRLCHTTGFNGLVLFMVIMSSFTLTLDDKYNSSNAQLMQVLRYMDIFYMAFFSMEMFLKMIGLGLHKYFTSFWTLLDCFLVLVSWLNQLASA-VPALK-SLRSLRTLRALRPLRAISRWKGMRLVVNALVTSIPSISNVMLMCLLFWMVFGIAGVQLFGGKFFR 1176 HSP 8 Score: 50.447 bits (119), Expect = 4.463e-6 Identity = 32/92 (34.78%), Postives = 50/92 (54.35%), Query Frame = 2
Query: 2315 ELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRL-YFSEKWNVFDFLIVVLSIVELTASKDISASKGNLS 2587
E T I LN V ++ + Y S S I +NL F +F EA +K++ G R+ YF + W++FDF +V +S+ E+ + AS+ N S
Sbjct: 1342 EYISTAVISLNMVMLASEHYKQSESFTNITKIINLVFTGLFMLEAALKIL--GNRIHYFRQPWDIFDFFVVCISLTEIVL--ETMASQVNFS 1429
BLAST of Sodium channel protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002291.1 (pep scaffold:Pmarinus_7.0:GL476946:177790:226078:1 gene:ENSPMAG00000002078.1 transcript:ENSPMAT00000002291.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 480.33 bits (1235), Expect = 2.512e-141 Identity = 262/547 (47.90%), Postives = 394/547 (72.03%), Query Frame = 2
Query: 3857 KIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDS-NNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNF-QPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSI-GFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDK--------KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ F GKF KCV+S ++ ++ES++ N++ C L+++ ++ W N +NFDNV +GYL+L Q++TFKGW DIM AVD + N QP++ + YY++FV+FIIFGAFF LNLFIGVIIDNFN QKKK GG ++F+T+ Q++Y++AMKK+ S P+KPVPRP + + Y++++ Q F+I IM I LN + M +E+ +Q + + AL+ N VF+ IF ECV+K+ ALR Y+F PWNVFDF +V S+ + + + N V PTL RV+R+FR+GR+LRL++ A+GIRTLLF+L++SLPALFNIGLLL ++MFI+++ G+S F + ++ GID FNFETFG +M+ LFQI+TS GW+ +L + + +C+ + CG+ + + V+Y++ISFL+++NMYIAVILENF+ AT + L++DD+++FY+ W ++DP AT F+ +L F+ + EPL +P+PN++ +A++++PI +G I C++++ LTK LG+
Sbjct: 1066 QVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNFFKGKFSKCVNSTDDSTMNESIIPNQNAC--LEQSPSLRWKNLPVNFDNVASGYLALLQVSTFKGWMDIMYAAVDSRQNNTEQPRFEESLGYYLFFVIFIIFGAFFILNLFIGVIIDNFNQQKKKFGGE-DIFLTEEQRKYHNAMKKLGSKKPRKPVPRPNNKYQGMAYDLVHWQAFEIGIMVLICLNMLTMMIEADEQSEAMKTALNITNIVFIGIFTTECVLKMFALRQYFFTFPWNVFDFAVVTLSVFDLAMADNSWLSNIVEPTLFRVVRLFRVGRILRLIRGAQGIRTLLFALLMSLPALFNIGLLLFVVMFIFSVFGMSNFGYLKHTAGIDEIFNFETFGGSMLCLFQITTSAGWDGLLAPTLLSKEPDCEPEAVHVGTNVVGNCGNKAIGCAFFVTYIIISFLIVVNMYIAVILENFNVATAESSEPLSEDDFEMFYDIWGKFDPNATQFISFTELPNFIDSLDEPLRVPKPNQLAVAVMDLPIVEGDRIHCLEVLFALTKRVLGQ 1609 HSP 2 Score: 298.516 bits (763), Expect = 6.934e-82 Identity = 173/393 (44.02%), Postives = 247/393 (62.85%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK--VFDNSS-------------------------IGAETYEND---SILLWNIKMDYYNNPENW---QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEA + +P G+ +K + LEDLDP+Y +K TF+V+N KTIFRF++ KA LF+ FNP+R +I+ILTH+LF+ ++ TI+ NC+ M N+E + VE +FTAIY E ++K LARG FTYLRD WNWLD V+ +A + LSALR RV+RALKT++ +PG+KT+V +L+ ++ KL D +LT+F LSIFALI LQ+++G L +KCV+ + NS+ +G T + + + Y+ + +N+ +N C N + NN TC + + C + +NPN+GYT++DNFG + L FRLMTQDYWENLYQL +R +G V+MI+F+MVIF GSFYLIN+IL++VA SY
Sbjct: 56 LEADRTMPFVYGEV--PQKMISEPLEDLDPYYRNKMTFLVLNKGKTIFRFNACKALCLFTVFNPIRRISIKILTHTLFTILIMATILTNCVFMAINTELKVVEYVFTAIYMFEALVKILARGFFFGAFTYLRDPWNWLDLAVLVIACFTSI-PGFANLSALRMFRVMRALKTISVVPGLKTIVRALIQSVKKLSDVTILTLFCLSIFALIALQLFKGNLKNKCVRWPIPGNSNFINISQYLEDKEFYRKRKRSCNVTWLGNRTLQTGFSFPCVSARLSPSYFISTDNYYFHKNVPLLCSNSSENN----TCPDGFICFKA-GKNPNYGYTNYDNFGWAFLSLFRLMTQDYWENLYQLTLRASGKVYMIFFVMVIFLGSFYLINLILAVVAKSY 440 HSP 3 Score: 82.8037 bits (203), Expect = 7.801e-16 Identity = 62/225 (27.56%), Postives = 114/225 (50.67%), Query Frame = 2
Query: 3446 NCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFA----IGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV------DSNNVRLHESLVQNKSDCYELQKTENVSWVNSQ 4087
N C C+ + IR + KI+ H F I+ I+ + + F+ LK ++E++ FT I++ E ++K A G Y +D W WLD ++++A + F A+R R +R L+ +S G+K +V ALIQ++ + +V ++ L IF++I +QLF G KCV +SN + + + L ++K + +++ NV+W+ ++
Sbjct: 104 NACKALCLFTVFNPIRRISIKILTHTLFTILIMATILTNCV-------FMAINTELK-VVEYV---FTAIYMFEALVKILARGFFFGAFTYLRDPWNWLDLAVLVIACFTSIPGF--ANLSALRMFRVMRALKTISVVPGLKTIVRALIQSVKKLSDVTILTLFCLSIFALIALQLFKGNLKNKCVRWPIPGNSNFINISQYL-EDKEFYRKRKRSCNVTWLGNR 314 HSP 4 Score: 82.4185 bits (202), Expect = 9.156e-16 Identity = 71/291 (24.40%), Postives = 126/291 (43.30%), Query Frame = 2
Query: 3500 AFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYF--------KVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXG-AFF----------TLNLFIGVIIDNFNV 4315
A+ +V + FE I+++I + LT+ +E + +K L ++ F IF E VLK FA+ +F W DF +V +++ + + F+ +R R R LR + G++ ++ AL+ ++P++FN+ L+ V IFS+ GM FG ++ + ++ NF+ L LFQI T GW ++A + E + +P+ + + G AFF +N++I VI++NFNV
Sbjct: 1264 AYDLVHWQAFEIGIMVLICLNMLTMMIEAD--EQSEAMKTALNITNIVFIGIFTTECVLKMFALR-QYFFTFPWNVFDFAVVTLSVFDLAMADNSWLSNIVEPTLFRVVRLFRVGRILRLIRGAQGIRTLLFALLMSLPALFNIGLLLFVVMFIFSVFGMSNFG-----------------------------YLKHTAGIDEIFNFETFGGSMLCLFQITTSAGWDGLLAPTLLSKEPDCEPEAVHVGTNVVGNCGNKAIGCAFFVTYIIISFLIVVNMYIAVILENFNV 1522 HSP 5 Score: 60.077 bits (144), Expect = 6.514e-9 Identity = 50/152 (32.89%), Postives = 85/152 (55.92%), Query Frame = 2
Query: 2315 ELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISAS----KGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFG 2758
E+ I + I LN + M I+ + S +M +N N+ F+ IFT E +K+ A + +F+ WNVFDF +V LS+ +L + + S ++R FR+ RI +L + Q + L + +L A+ N+ ++L +++FIF+V GM FG
Sbjct: 1274 EIGIMVLICLNMLTMMIEADEQSEAMKTALNITNIVFIGIFTTECVLKMFALR-QYFFTFPWNVFDFAVVTLSVFDLAMADNSWLSNIVEPTLFRVVRLFRVGRILRLIRGAQGIRTLLFALLMSLPALFNIGLLLFVVMFIFSVFGMSNFG 1424
BLAST of Sodium channel protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002303.1 (pep scaffold:Pmarinus_7.0:GL476946:177796:226078:1 gene:ENSPMAG00000002078.1 transcript:ENSPMAT00000002303.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 481.871 bits (1239), Expect = 2.579e-141 Identity = 262/547 (47.90%), Postives = 394/547 (72.03%), Query Frame = 2
Query: 3857 KIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDS-NNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNF-QPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSI-GFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDK--------KKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ F GKF KCV+S ++ ++ES++ N++ C L+++ ++ W N +NFDNV +GYL+L Q++TFKGW DIM AVD + N QP++ + YY++FV+FIIFGAFF LNLFIGVIIDNFN QKKK GG ++F+T+ Q++Y++AMKK+ S P+KPVPRP + + Y++++ Q F+I IM I LN + M +E+ +Q + + AL+ N VF+ IF ECV+K+ ALR Y+F PWNVFDF +V S+ + + + N V PTL RV+R+FR+GR+LRL++ A+GIRTLLF+L++SLPALFNIGLLL ++MFI+++ G+S F + ++ GID FNFETFG +M+ LFQI+TS GW+ +L + + +C+ + CG+ + + V+Y++ISFL+++NMYIAVILENF+ AT + L++DD+++FY+ W ++DP AT F+ +L F+ + EPL +P+PN++ +A++++PI +G I C++++ LTK LG+
Sbjct: 1149 QVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNFFKGKFSKCVNSTDDSTMNESIIPNQNAC--LEQSPSLRWKNLPVNFDNVASGYLALLQVSTFKGWMDIMYAAVDSRQNNTEQPRFEESLGYYLFFVIFIIFGAFFILNLFIGVIIDNFNQQKKKFGGE-DIFLTEEQRKYHNAMKKLGSKKPRKPVPRPNNKYQGMAYDLVHWQAFEIGIMVLICLNMLTMMIEADEQSEAMKTALNITNIVFIGIFTTECVLKMFALRQYFFTFPWNVFDFAVVTLSVFDLAMADNSWLSNIVEPTLFRVVRLFRVGRILRLIRGAQGIRTLLFALLMSLPALFNIGLLLFVVMFIFSVFGMSNFGYLKHTAGIDEIFNFETFGGSMLCLFQITTSAGWDGLLAPTLLSKEPDCEPEAVHVGTNVVGNCGNKAIGCAFFVTYIIISFLIVVNMYIAVILENFNVATAESSEPLSEDDFEMFYDIWGKFDPNATQFISFTELPNFIDSLDEPLRVPKPNQLAVAVMDLPIVEGDRIHCLEVLFALTKRVLGQ 1692 HSP 2 Score: 307.76 bits (787), Expect = 1.046e-84 Identity = 175/363 (48.21%), Postives = 243/363 (66.94%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK--VFDNSS-IGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEA + +P G+ +K + LEDLDP+Y +K TF+V+N KTIFRF++ KA LF+ FNP+R +I+ILTH+LF+ ++ TI+ NC+ M N+E + VE +FTAIY E ++K LARG FTYLRD WNWLD V+ +A + LSALR RV+RALKT++ +PG+KT+V +L+ ++ KL D +LT+F LSIFALI LQ+++G L +KCV+ + NS+ I Y D K +YY + +N C N + NN TC + + C + +NPN+GYT++DNFG + L FRLMTQDYWENLYQL +R +G V+MI+F+MVIF GSFYLIN+IL++VA SY
Sbjct: 54 LEADRTMPFVYGEV--PQKMISEPLEDLDPYYRNKMTFLVLNKGKTIFRFNACKALCLFTVFNPIRRISIKILTHTLFTILIMATILTNCVFMAINTELKVVEYVFTAIYMFEALVKILARGFFFGAFTYLRDPWNWLDLAVLVIACFTSI-PGFANLSALRMFRVMRALKTISVVPGLKTIVRALIQSVKKLSDVTILTLFCLSIFALIALQLFKGNLKNKCVRWPIPGNSNFINISQYLED-------KDNYYFH----KNVPLLCSNSSENN----TCPDGFICFKA-GKNPNYGYTNYDNFGWAFLSLFRLMTQDYWENLYQLTLRASGKVYMIFFVMVIFLGSFYLINLILAVVAKSY 397 HSP 3 Score: 88.5817 bits (218), Expect = 1.295e-17 Identity = 84/328 (25.61%), Postives = 147/328 (44.82%), Query Frame = 2
Query: 3446 NCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFA----IGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV------------------DSNNVRLHES---LVQNKSD---------CYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKK 4324
N C C+ + IR + KI+ H F I+ I+ + + F+ LK ++E++ FT I++ E ++K A G Y +D W WLD ++++A + F A+R R +R L+ +S G+K +V ALIQ++ + +V ++ L IF++I +QLF G KCV D +N H++ L N S+ C++ K N + N+DN +LSLF++ T W ++ + + Y I+FV+ I G+F+ +NL + V+ ++ Q +
Sbjct: 102 NACKALCLFTVFNPIRRISIKILTHTLFTILIMATILTNCV-------FMAINTELK-VVEYV---FTAIYMFEALVKILARGFFFGAFTYLRDPWNWLDLAVLVIACFTSIPGF--ANLSALRMFRVMRALKTISVVPGLKTIVRALIQSVKKLSDVTILTLFCLSIFALIALQLFKGNLKNKCVRWPIPGNSNFINISQYLEDKDNYYFHKNVPLLCSNSSENNTCPDGFICFKAGKNPNYGYT----NYDNFGWAFLSLFRLMTQDYWENLYQLTLRAS----------GKVYMIFFVMVIFLGSFYLINLILAVVAKSYEEQNQ 402 HSP 4 Score: 82.8037 bits (203), Expect = 6.848e-16 Identity = 71/291 (24.40%), Postives = 126/291 (43.30%), Query Frame = 2
Query: 3500 AFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYF--------KVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXG-AFF----------TLNLFIGVIIDNFNV 4315
A+ +V + FE I+++I + LT+ +E + +K L ++ F IF E VLK FA+ +F W DF +V +++ + + F+ +R R R LR + G++ ++ AL+ ++P++FN+ L+ V IFS+ GM FG ++ + ++ NF+ L LFQI T GW ++A + E + +P+ + + G AFF +N++I VI++NFNV
Sbjct: 1347 AYDLVHWQAFEIGIMVLICLNMLTMMIEAD--EQSEAMKTALNITNIVFIGIFTTECVLKMFALR-QYFFTFPWNVFDFAVVTLSVFDLAMADNSWLSNIVEPTLFRVVRLFRVGRILRLIRGAQGIRTLLFALLMSLPALFNIGLLLFVVMFIFSVFGMSNFG-----------------------------YLKHTAGIDEIFNFETFGGSMLCLFQITTSAGWDGLLAPTLLSKEPDCEPEAVHVGTNVVGNCGNKAIGCAFFVTYIIISFLIVVNMYIAVILENFNV 1605 HSP 5 Score: 60.077 bits (144), Expect = 5.550e-9 Identity = 50/152 (32.89%), Postives = 85/152 (55.92%), Query Frame = 2
Query: 2315 ELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISAS----KGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFG 2758
E+ I + I LN + M I+ + S +M +N N+ F+ IFT E +K+ A + +F+ WNVFDF +V LS+ +L + + S ++R FR+ RI +L + Q + L + +L A+ N+ ++L +++FIF+V GM FG
Sbjct: 1357 EIGIMVLICLNMLTMMIEADEQSEAMKTALNITNIVFIGIFTTECVLKMFALR-QYFFTFPWNVFDFAVVTLSVFDLAMADNSWLSNIVEPTLFRVVRLFRVGRILRLIRGAQGIRTLLFALLMSLPALFNIGLLLFVVMFIFSVFGMSNFG 1507
BLAST of Sodium channel protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007955.1 (pep scaffold:Pmarinus_7.0:GL476358:329878:417380:1 gene:ENSPMAG00000007100.1 transcript:ENSPMAT00000007955.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 314.694 bits (805), Expect = 1.129e-86 Identity = 183/371 (49.33%), Postives = 249/371 (67.12%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHS----LFSFFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVK-VFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHN--FTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LE GK LP GD P+ + LEDLDPFY+ ++TF+V+NS K IFRFS+T A ++ +PFNP+R AI+ILTHS LFS F++ TI+ NC+ MT + ++NVE FT IYT E ++K LARG ++ FT+LRD WNWLDF VI +AY+ +++ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ A+ +L D ++LTVF LS+FALIGLQ++ G L HKC+ N + E E W +Y NN +N Q + + N +C E++ C++ NPN+GYTSFD F + L FRLMTQDYWENLYQL +R G + I+FM+VIF GSFYLIN+IL++VAM+Y
Sbjct: 53 LEVGKSLPFMYGDMPPE--CVAEPLEDLDPFYSTQKTFIVLNSGKAIFRFSATPALYILTPFNPVRRYAIKILTHSWQRTLFSIFIMFTILTNCVFMTMSEPPTWTKNVEYTFTGIYTFESMVKILARGFCLHPFTFLRDPWNWLDFCVIVMAYVTEFID--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQAVKQLSDVMILTVFCLSVFALIGLQLFMGNLRHKCILWPIANQTNVTEFNETAEPFNWE---NYVNNEDN-QYKLEGSKDALLCGNGSDAGSCPEKFVCVK-GGRNPNYGYTSFDTFSWAFLSLFRLMTQDYWENLYQLTLRAAGKPY-IFFMVVIFLGSFYLINLILAVVAMAY 413 HSP 2 Score: 257.684 bits (657), Expect = 3.366e-69 Identity = 127/242 (52.48%), Postives = 176/242 (72.73%), Query Frame = 2
Query: 3473 KAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKV---FDA-GAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD-SNNVRLHESLVQNKSDCYELQKTENVS-WVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVD 4180
++WW +R + ++IV+H +FE+ I+ MI+ SS L ED ++ RK +K +LE+ D FT +F++EM+LKW A G KYF +AWCWLDF IV ++++S +D A K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSI NVLLVCL+FWLIFSI+G+ LF GK++ C++ ++ RL S+V N S C L T N + W N ++NFDNV GYL+L Q+ATFKGW DIM AVD
Sbjct: 1186 RSWWNLRRACYRIVEHNWFESVIVFMILLSSGALAFEDIYIEQRKTIKTVLEYADKVFTYVFILEMLLKWVAYGFQKYFTNAWCWLDFLIVDISIVSLMATALGYDQLSAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFSGKYYYCLNKTDGQRLPISIVDNYSMCIALNDTTNETLWKNVKVNFDNVGIGYLALLQVATFKGWMDIMYAAVD 1427 HSP 3 Score: 236.498 bits (602), Expect = 1.245e-62 Identity = 127/249 (51.00%), Postives = 182/249 (73.09%), Query Frame = 2
Query: 4742 VSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
VSPTL RVIR+ RIGR+LRL++ A+GIRTLLF+L++SLPALFNIGLLL L+MFIY+I G+S F+ V GID FNFETFG +MI LFQI+TS GW+ +L ++N+ +C + K CG+ ++ +++ VSY++ISFLV++NMYIA+ILENFS AT++ L++DD+++FYE WE++DP AT F+ + L +F + EPL +P+PNKI+L +++P+ I C+D++ TK LGE
Sbjct: 1568 VSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGVPDCDPEIEHPGSLVKGDCGNPMVGIIFFVSYIIISFLVVVNMYIAIILENFSVATEESSEPLSEDDFEMFYEIWEKFDPDATQFIAWERLPDFADALDEPLRVPKPNKIKLIAMDLPMVINDRIHCLDILFAFTKRVLGE 1816 HSP 4 Score: 216.083 bits (549), Expect = 1.902e-56 Identity = 149/310 (48.06%), Postives = 211/310 (68.06%), Query Frame = 2
Query: 2222 RFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKK 3148
+F + WECC + +K+I+ IV+DPF++L ITICI+LNT+FM+++ Y + + NL F IFT E +K+ A P YF + WN+FD +IV LS++EL S S LS+LR+FRLLR+FKLAKSW T+N+L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + +N +PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G C+ F+ M++GNLV+LNLFLALLLSSFS ++L +D+ ++N LQ A+ R+ R + ++K + + K+
Sbjct: 724 KFANKYLIWECCSLWMKVKEILKMIVMDPFVDLSITICIILNTLFMALEHYPMTPEFDNALFVGNLVFTGIFTAEMVLKIAAQDPYYYFQQGWNIFDSIIVSLSLMELGLS-----SVDGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKMYKECVCKI---SENCTLPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMVIGNLVVLNLFLALLLSSFSGDNLSATDDD-GEMNNLQVAIGRIERGVSFIKRSICDVFRKALKR 1024 HSP 5 Score: 87.0409 bits (214), Expect = 4.228e-17 Identity = 64/217 (29.49%), Postives = 113/217 (52.07%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKF----FETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESL---VQNKSDCYELQKT-ENVSWVNSQINFDN 4102
+R A KI+ H + F FI+ I+ + + +T+ + T+ ++ FT I+ E ++K A G + +D W WLDF ++++A ++ F D G A+RT R LR L+ +S G+K +V ALIQA+ + +V+++ + +F++IG+QLF G N+R H+ + + N+++ E +T E +W N N DN
Sbjct: 115 VRRYAIKILTHSWQRTLFSIFIMFTILTNCVFMTMSEPPTWTKN--------VEYTFTGIYTFESMVKILARGFCLHPFTFLRDPWNWLDFCVIVMAYVTEF--IDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQAVKQLSDVMILTVFCLSVFALIGLQLFMG---------NLR-HKCILWPIANQTNVTEFNETAEPFNWENYVNNEDN 311 HSP 6 Score: 70.8626 bits (172), Expect = 3.131e-12 Identity = 51/180 (28.33%), Postives = 84/180 (46.67%), Query Frame = 2
Query: 3449 CCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCV 3988
CCS W ++ IV F + I I I+ ++L + LE P + L +L FT IF EMVLK A YF+ W D IV ++L+ + +R+ R LR + W + +++ + ++ ++ N+ LV + IF+++GMQLFG + +CV
Sbjct: 734 CCS------LWMKVKEILKMIVMDPFVDLSITICIILNTLFMALE--HYPMTPEFDNALFVGNLVFTGIFTAEMVLKIAAQDPYYYFQQGWNIFDSIIVSLSLME-LGLSSVDGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKMYKECV 904 HSP 7 Score: 66.6254 bits (161), Expect = 7.165e-11 Identity = 54/176 (30.68%), Postives = 85/176 (48.30%), Query Frame = 2
Query: 4442 IAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALR-WYYFKIPWNVFDFIIVIFSI---------GFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEV 4939
+ +I I+ + D+ I I+LNT+ M LE + E +AL N VF IF E V+KI A +YYF+ WN+FD IIV S+ G V+ F + L KS + L+ + S+ AL N+ L+L +I+FI+A++G+ +F ++
Sbjct: 741 VKEILKMIVMDPFVDLSITICIILNTLFMALEHYPMTPEFDNALFVGNLVFTGIFTAEMVLKIAAQDPYYYFQQGWNIFDSIIVSLSLMELGLSSVDGLSVLRSFRLLRVFK-----------------LAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKM 899 HSP 8 Score: 58.5362 bits (140), Expect = 1.913e-8 Identity = 55/212 (25.94%), Postives = 110/212 (51.89%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALG-----KXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQN 1186
LR A RI+ H+ F ++ I+++ A+ T + E + +FT ++ E ++K +A G +Q Y +AW WLDF+++ ++ + + +ALG + +LR+LR LR L+ ++ GM+ VV++L+ AI + + L++ + IF+++G+ ++ G + C+ D + +N S+ I ++ N W+N
Sbjct: 1191 LRRACYRIVEHNWFESVIVFMILLSSGALAFEDIYIEQRKTIKTVLEYADKVFTYVFILEMLLKWVAYG---FQ-KYFTNAWCWLDFLIVDISIVSLMATALGYDQLSAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFSGKYYY-CLNKTDGQRLPISIVDNYSMC---IALNDTTNETLWKN 1394 HSP 9 Score: 55.4546 bits (132), Expect = 1.607e-7 Identity = 48/219 (21.92%), Postives = 91/219 (41.55%), Query Frame = 2
Query: 3791 FKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFF-----------TLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQ 4414
F+ IR R R LR + G++ ++ AL+ ++P++FN+ L+ + I+SI GM F + V+ +S ++ NF+ N + LFQI T GW ++A ++ + P+ + + G F +N++I +I++NF+V ++ S E D + +Y +K + Q
Sbjct: 1573 FRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNF-----------------AYVKKESG------------IDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSGVPDCDPEIEHPGSLVKGDCGNPMVGIIFFVSYIIISFLVVVNMYIAIILENFSVATEE---SSEPLSEDDFEMFYEIWEKFDPDATQ 1759 HSP 10 Score: 54.6842 bits (130), Expect = 2.685e-7 Identity = 48/151 (31.79%), Postives = 74/151 (49.01%), Query Frame = 2
Query: 2258 PAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYG----PRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNL 2698
P Y KI++ +FI I+ N VFM++ S + N V F I+TFE+ +K++A G P + + WN DF ++V++ V T D+ GN+S LRTFR+LR K+ + L TI+G + A+ L
Sbjct: 114 PVRRYAIKILTHSWQRTLFSIFIMFTILTNCVFMTM-----SEPPTWTKN-VEYTFTGIYTFESMVKILARGFCLHPFTFLRDPWNWLDFCVIVMAYV--TEFIDL----GNVSALRTFRVLRAL---KTISVIPGLKTIVGALIQAVKQL 249 HSP 11 Score: 50.0618 bits (118), Expect = 7.575e-6 Identity = 49/184 (26.63%), Postives = 90/184 (48.91%), Query Frame = 2
Query: 2273 LKKIVSFIVLDPFMELFITICIVLNTVFMSI-DTY-DNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIP 2818
L++ IV + E I I+L++ ++ D Y + ++ ++ + F +F E +K +AYG + YF+ W DFLIV +SIV L A+ + LRT R LR + ++ M ++ + + +I N+ +V I IF+++G+ +F +Y N+T D ++P
Sbjct: 1191 LRRACYRIVEHNWFESVIVFMILLSSGALAFEDIYIEQRKTIKTVLEYADKVFTYVFILEMLLKWVAYGFQKYFTNAWCWLDFLIVDISIVSLMATALGYDQLSAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFSGKYYYCLNKT----DGQRLP 1370
BLAST of Sodium channel protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000617.1 (pep scaffold:Pmarinus_7.0:GL477322:572:57208:-1 gene:ENSPMAG00000000554.1 transcript:ENSPMAT00000000617.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 280.796 bits (717), Expect = 1.626e-79 Identity = 164/369 (44.44%), Postives = 237/369 (64.23%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRT--FVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTH-SLFSFFVLTTIIINCIAMTKNSE------SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEA + LP GD K+ + LEDLDPFY ++ T F+V+N K I+RF++T + +LFSPFNP+R AI+IL H F+ ++ TI+ NC+ M + + ++N IY E +IK LARG + QFT+LRD WNWLD +V+ + YVN K+S+L++ RVLR LKT++ PG+KT+V +L+ ++ KL D ++LT+F LSIFALIG Q++ G L +KCV ++ N G T++N+++ +YY + N C N + C E Y C++ ENPNFG+T++D+FG + L FRLMTQD WE LYQ +R++G V+MI+F+MVIF GSFYLIN+IL++VA SY
Sbjct: 57 LEAERKLPFVYGDV--PKELISEPLEDLDPFYRNQMTSPFLVMNRKKRIYRFNATNSLYLFSPFNPVRRIAIKILIHIYPFTMVIVGTILTNCVFMAMDKKLNMPLWNKNSRYTMNTIYMMEVVIKVLARGFFMGQFTFLRDPWNWLDLLVVIFTIVTYVNPK-QKMSSLKTFRVLRVLKTISVFPGLKTIVKALIQSVKKLFDVIILTIFCLSIFALIGQQLFMGNLKNKCV-LWPN---GNRTWDNETLAYLKDTNNYYKS----DNQPILCGNSSG----AGECPEGYLCLKI-GENPNFGFTNYDHFGWAFLSLFRLMTQDSWEYLYQQTLRSSGKVYMIFFVMVIFLGSFYLINLILAVVAKSY 409 HSP 2 Score: 101.679 bits (252), Expect = 8.035e-22 Identity = 53/122 (43.44%), Postives = 75/122 (61.48%), Query Frame = 2
Query: 2246 WECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLL 2611
WE CP + +K++ + DPFM+L IT+CIVLNTVFMS++ S ++ N+FF FT E +K+IA P YF EKWN FD ++V +S+VE+ NLS+LR+FRL+
Sbjct: 707 WEWCPVWIRIKRLAKLVASDPFMDLAITLCIVLNTVFMSLEHDSMSEDFKKVLRNGNVFFTGTFTAEMVLKIIAMDPYYYFQEKWNQFDSVVVTVSLVEMWL-----PDFDNLSVLRSFRLV 823 HSP 3 Score: 77.411 bits (189), Expect = 2.245e-14 Identity = 77/310 (24.84%), Postives = 149/310 (48.06%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV------------------DSNNVRLHES---LVQNKS---DCYE----LQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKI 4330
+R A KI+ H + T +++ + ++ DK L K+ M+ + + +++++ + F +G + +D W WLD +V+ +++Y V +++T R LR L+ +S + G+K +V ALIQ++ +F+V+++ + IF++IG QLF G KCV D+NN ++ L N S +C E L+ EN ++ N+D+ +LSLF++ T W + + ++ Y I+FV+ I G+F+ +NL + V+ ++ Q + +
Sbjct: 121 VRRIAIKILIHIYPFTMVIVGTILTNCVFMAMDKKLNMPLWNKNSRYTMNTIYMMEVVIKVLARGFFMGQFTFLRDPWNWLDLLVVIFTIVTY--VNPKQKMSSLKTFRVLRVLKTISVFPGLKTIVKALIQSVKKLFDVIILTIFCLSIFALIGQQLFMGNLKNKCVLWPNGNRTWDNETLAYLKDTNNYYKSDNQPILCGNSSGAGECPEGYLCLKIGENPNF--GFTNYDHFGWAFLSLFRLMTQDSWEYLYQQTL----------RSSGKVYMIFFVMVIFLGSFYLINLILAVVAKSYEDQNRAM 416 HSP 4 Score: 51.6026 bits (122), Expect = 1.881e-6 Identity = 28/74 (37.84%), Postives = 41/74 (55.41%), Query Frame = 2
Query: 4484 DIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIAL-RWYYFKIPWNVFDFIIVIFSI 4702
D+ I I+LNT+ M LE ++ L + N F F E V+KIIA+ +YYF+ WN FD ++V S+
Sbjct: 730 DLAITLCIVLNTVFMSLEHDSMSEDFKKVLRNGNVFFTGTFTAEMVLKIIAMDPYYYFQEKWNQFDSVVVTVSL 803
BLAST of Sodium channel protein vs. Ensembl Yeast
Match: CCH1 (Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function [Source:SGD;Acc:S000003449]) HSP 1 Score: 135.576 bits (340), Expect = 4.712e-32 Identity = 141/601 (23.46%), Postives = 260/601 (43.26%), Query Frame = 2
Query: 3641 GFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVL---VALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVN-ALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSW------VNSQ--INFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPR--PKSTIAQIFYNIINNQK---FDIVIMCFIMLNTIVMCLESHKQGKELS-DALDHM--NKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFS-----IGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK----CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKD--VQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLG 5350
F++ F+V+ V F Y ++ W ++DF +++ + LI+Y K + + + L ALR LR ++ + + N + + IF L+ L F++ G+ +F G+ C D + ++DCY W V Q ++ D+ + + SL+QI + +GW D++ + ++ + + + ++ VLF F LNLF+ I++N Q + G + + T +K + + K +S P K +P S + Q FY + +K + + + L+ I++ S+ G + + M VF++ AL + L YF+ WN I+I + + F V F+ + F + L ++ + LL + + SLP + ++ ++ +YAI +F NF T K+MI+LF+ S GWN I+ L + Y C D CGS A L L+S+ +IS + +NM++++I+ NFS + + G+ + + + E W ++D TG +EL L + PL
Sbjct: 1252 AFSIEFIVKTVADGFIYSPNAYLRNPWNFIDFCVLISMWINLIAYLKN-NGNLSRIFKGLTALRALRCLTISNTARQTFNLVMFDGLNKIFEAGLISLSLLFPFTVWGLSIFKGRLGTCNDGS---------LGRADCYNEYSNSVFQWDIMSPRVYQQPYLHLDSFASAFSSLYQIISLEGWVDLLENMMNSSGIGTPATVMGSAGNALFLVLFNFLSMVFILNLFVSFIVNN---QARTTGSA---YFTIEEKAWLESQKLLSQAKP-KAIPNLIELSRVRQFFYQLAVEKKNFYYASFLQVVLYLHIIMLLSRSYNPGNLIGYQGVYFMFSTSVFLIQEALHMCGEGPRL---YFRQKWNSIRLSIIIIAFIMNAVAFHVPASHYWFHNIK-------GFFLLVIFLFIIPQNDTLTELLETAMASLPPILSLTYTWGVLFLVYAIALNQIFGLTRLGSNTTDNINFRTVIKSMIVLFRCSFGEGWNYIMADLTVSEPY-CSSDDNSTYTDCGSETYAYLLLMSWNIISMYIFVNMFVSLIIGNFSYVYRSGGSRSGINRSEIKKYIEAWSKFDTDGTGELELSYLPRIMHSFDGPLS 1824 HSP 2 Score: 53.1434 bits (126), Expect = 4.763e-7 Identity = 40/184 (21.74%), Postives = 89/184 (48.37%), Query Frame = 2
Query: 938 GMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKS-TCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
GM +++ L I +L + + V+ F ++G+Q+++G+ +CV + N +TY+ D Y +P + + + +V K C + +C+ N NP G SFDN S+ F +M+ + + +L + ++ ++F++ IF + +L+N++++++ S+
Sbjct: 542 GMPSILRGLKYGIPQLVNVSSMLVYFWIFFGILGVQIFQGSFRRQCV--WFNPEDPTDTYQYDMQFCGG-----YLDPVTKRKQNYIYEDGSEGSVSKGFLCPQYSKCV--SNANPYNGRISFDNIVNSMELVFVIMSANTFTDLMYYTMDSDEMAACLFFIVCIFVLTIWLLNLLIAVLVSSF 716
BLAST of Sodium channel protein vs. Ensembl Nematostella
Match: EDO35661 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKR1]) HSP 1 Score: 566.229 bits (1458), Expect = 2.077e-172 Identity = 323/695 (46.47%), Postives = 468/695 (67.34%), Query Frame = 2
Query: 3425 CFLVRHCNC---CSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVA-LISYFKVF--DAGAF-KAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIG--GSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYEC-----KIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEF 5467
CF C C C +S W +R ++V+HK FE IL +I ASS++L ED +L ++ LK +L+ +++ F VIF VEM+LKW +G YF + W LDF IV+V+ ++S +F D AF +++RTLRA RPLRA+SR++GMK+V+ +L+ AIP I NVLLVCL+FWLIFSI+G+Q+FGGKF KCVD + S+V NK+ C + W NS INFD V G+L+L Q+ATF+GW +IM DAVD T+++ QP NN Y++FV+FII G FFTLNLFIGVIIDNFN K+++ GS+++F+T +Q+ + +A+K ++ P+K + RP++ Y+++ ++KF++ IM FI +N +VM ++ + S + +N +F +F LE +++IIALR YF PWNVFDF+IVI SI ++E ++P+L+RV+RVFR+GR+LR ++ARGIR LLFSLV+S PALFNIG LL LI+FIYAIIG+S+F V + ++ NFETFG + ++LF++ TS GWN ILD LM + +C + CG+ +A +YL S++VI FL++INMYIAVILEN++Q + ++G+T +D +LFY+ WE +DP AT ++ DLS+FV ++ L IP+PNK A+L IP+ KG I C+DL+ L K + F
Sbjct: 792 CFPTSWCVCRLACMDSGFCAVWKVMRYKVRRLVEHKVFEGIILFLIAASSISLAFEDVYLDSKPTLKQVLQILNILFAVIFTVEMLLKWIGLGFKTYFTNPWNILDFVIVIVSIMVSLATIFGNDQIAFIRSLRTLRAFRPLRAISRFEGMKVVITSLLHAIPGIGNVLLVCLMFWLIFSIMGVQIFGGKFGKCVDEGGEKCPASVVPNKTVCL----SNGYRWENSNINFDTVDQGFLALLQVATFEGWMEIMEDAVDATKIDEQPIEENNVSAYLFFVVFIILGTFFTLNLFIGVIIDNFNQLKQQMEAVGSMDVFLTSTQRNWMNALKSAATKKPKKRIRRPQNKFQGELYDMVQSRKFEVFIMLFITINMVVMMVQHYDHLAVWSKQI--LNLIFTSVFILEAILRIIALRKGYFLNPWNVFDFVIVISSIIGIIVENLQTSLVINPSLLRVVRVFRVGRLLRFFEAARGIRRLLFSLVISTPALFNIGALLFLIIFIYAIIGMSIFGHVKKTEALNDVVNFETFGSSFVLLFRLMTSAGWNDILDPLMISEP-DCDPNYRGLPSGNCGNKYMAPIYLGSFVVIIFLILINMYIAVILENYNQVMEQEKIGITNEDIELFYQMWELFDPNATQYIPYADLSDFVHQMEGNLRIPKPNKAACALLNIPLVKGDKIHCLDLLQALVKRIVSGF 1479 HSP 2 Score: 225.328 bits (573), Expect = 3.724e-59 Identity = 131/335 (39.10%), Postives = 211/335 (62.99%), Query Frame = 2
Query: 512 RTFVVINS--SKT-IFRFSSTKAFFLFSPFNPLRAAAIRILTHSL------FSFFVLTTIIINCIAMTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKC-VKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
++F+VI KT ++RF+ +K+ +LF P NP+R +++++T+ FVL TI++NC+ + + E E +F AIYT E K +A+G ++++ YLRD WNWLDFIV+ L + ++ + LS + + RV RAL+T++ + G+K +V++LL+++ L D +VLT+F + IFALIG+Q++ G L +KC + V +N S+ TY ++S + W ++ N C N T + TC Y C+ NPNFGYTSFD+FG +LL F+L+T D+WEN+Y +I T G +++YF +VIFFG F+L+N++L++VA SY
Sbjct: 1 QSFLVIAGKFGKTQVYRFNRSKSLYLFGPENPIRQFSLKLITNQYPLQLHNRHMFVLLTILVNCVFLAMTNPPEQPEYVFAAIYTIEMFCKIIAKGFALHRYAYLRDKWNWLDFIVVILGNVT-ISPDVANLSGIPTFRVFRALRTISAVKGLKAMVNTLLVSMKMLWDVMVLTLFFICIFALIGMQLFIGELRNKCALPVPENLSVPYRTYASNSSI-WYLE----------DNEPILCGNTTGSR----TCPTNYTCLPHAGGNPNFGYTSFDHFGWALLTGFQLITLDFWENVYNNVIYTMGPWYVVYFAVVIFFGPFFLVNLVLAVVAASY 319 HSP 3 Score: 180.259 bits (456), Expect = 2.234e-45 Identity = 103/214 (48.13%), Postives = 140/214 (65.42%), Query Frame = 2
Query: 2312 MELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKG-NLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLAT 2950
ME+FI +CI+LNT+ MSI+ ++ ++N N F IF E +KLIA G Y WN+FD ++V++SIV+ +K + + G +S+LRTFRLLR+ KLAKSW TMN L IG++LGA+GNL ++LAIIV+IFAV+GMQ+ G Y K G IPRWNF DF HSFM++FRVLCGE++E +WDCM G + F+
Sbjct: 505 MEIFIIVCILLNTLVMSIEHPRLEDPLLTVVNISNEVFTFIFLLEMILKLIALGFLGYIRVAWNIFDGIVVIISIVDFIVNKFVPDAGGTGISVLRTFRLLRVLKLAKSWSTMNSLLATIGKSLGALGNLTVILAIIVYIFAVMGMQLLGNSYTP-------DKFGGSIPRWNFKDFPHSFMMIFRVLCGEWIEPLWDCMLATGPVAMLLFVPA 711 HSP 4 Score: 79.7221 bits (195), Expect = 1.021e-14 Identity = 68/195 (34.87%), Postives = 115/195 (58.97%), Query Frame = 2
Query: 4469 NNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGID------ATFNFETFGKAMIILFQISTSGGW 5032
N + +I I+ I+LNT+VM +E + L ++ N+VF IF LE ++K+IAL + Y ++ WN+FD I+VI SI +++ KFV T I V+R FR+ RVL+L KS + +LL ++ SL AL N+ ++L +I++I+A++G+ + SY D +NF+ F + +++F++ G W
Sbjct: 501 NGKVMEIFIIVCILLNTLVMSIEHPRLEDPLLTVVNISNEVFTFIFLLEMILKLIALGFLGYIRVAWNIFDGIVVIISIVDFIVNKFV--PDAGGTGISVLRTFRLLRVLKLAKSWSTMNSLLATIGKSLGALGNLTVILAIIVYIFAVMGMQLLGN---SYTPDKFGGSIPRWNFKDFPHSFMMIFRV-LCGEW 689 HSP 5 Score: 76.6406 bits (187), Expect = 8.838e-14 Identity = 46/163 (28.22%), Postives = 87/163 (53.37%), Query Frame = 2
Query: 3503 FKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFK---VFDAG--AFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF 3976
K + K E FI++ I+ ++L +++E L L ++ + FT IFL+EM+LK A+G Y + AW D +V+++++ + V DAG +RT R LR L+ W M ++ + +++ ++ N+ ++ + IF+++GMQL G +
Sbjct: 497 LKFTNGKVMEIFIIVCILLNTLVMSIEHPRL--EDPLLTVVNISNEVFTFIFLLEMILKLIALGFLGYIRVAWNIFDGIVVIISIVDFIVNKFVPDAGGTGISVLRTFRLLRVLKLAKSWSTMNSLLATIGKSLGALGNLTVILAIIVYIFAVMGMQLLGNSY 657 HSP 6 Score: 73.559 bits (179), Expect = 8.605e-13 Identity = 40/115 (34.78%), Postives = 65/115 (56.52%), Query Frame = 2
Query: 3644 FTVIFLVEM----VLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF 3976
F I+ +EM + K FA+ Y +D W WLDF +V++ ++ D I T R R LR +S G+K +VN L+ ++ +++V+++ L F IF++IGMQLF G+
Sbjct: 80 FAAIYTIEMFCKIIAKGFALHRYAYLRDKWNWLDFIVVILGNVTISP--DVANLSGIPTFRVFRALRTISAVKGLKAMVNTLLVSMKMLWDVMVLTLFFICIFALIGMQLFIGEL 192 HSP 7 Score: 67.0106 bits (162), Expect = 7.685e-11 Identity = 63/251 (25.10%), Postives = 115/251 (45.82%), Query Frame = 2
Query: 2240 CNWEC-----CPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSI-DTY-DNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIP----------RW-----NFCDFTHSFMIVFRVLCGE-YMESMWDCMRVNGI 2923
C C C + ++ V +V E I I +++ ++ D Y D+ ++ ++ +N+ F VIFT E +K I G + YF+ WN+ DF+IV++SI+ A+ + + LRT R R + ++ M ++ T + + IGN+ +V + IF+++G+QIFG ++ + ++ +P RW NF F+ + +V E +ME M D + I
Sbjct: 800 CRLACMDSGFCAVWKVMRYKVRRLVEHKVFEGIILFLIAASSISLAFEDVYLDSKPTLKQVLQILNILFAVIFTVEMLLKWIGLGFKTYFTNPWNILDFVIVIVSIMVSLATIFGNDQIAFIRSLRTLRAFRPLRAISRFEGMKVVITSLLHAIPGIGNVLLVCLMFWLIFSIMGVQIFGGKFGKCVDEGGEKCPASVVPNKTVCLSNGYRWENSNINFDTVDQGFLALLQVATFEGWMEIMEDAVDATKI 1050 HSP 8 Score: 54.299 bits (129), Expect = 5.665e-7 Identity = 42/176 (23.86%), Postives = 83/176 (47.16%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAM-----------TKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXX-----XKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCV 1078
+R R++ H +F +L I + I++ T + + +F I+T E ++K + G TY + WN LDF+++ ++ M+ + + G + L+ ++ GMK V++SLL AI + + L++ + IF+++G+Q++ G KCV
Sbjct: 816 MRYKVRRLVEHKVFEGIILFLIAASSISLAFEDVYLDSKPTLKQVLQILNILFAVIFTVEMLLKWIGLGFK----TYFTNPWNILDFVIVIVSIMVSLATIFGNDQIAFIRSLRTLRAFRPLRAISRFEGMKVVITSLLHAIPGIGNVLLVCLMFWLIFSIMGVQIFGGK-FGKCV 986
BLAST of Sodium channel protein vs. Ensembl Nematostella
Match: EDO46797 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RP56]) HSP 1 Score: 476.478 bits (1225), Expect = 7.352e-141 Identity = 281/703 (39.97%), Postives = 429/703 (61.02%), Query Frame = 2
Query: 3401 DCLPDSIRCFLVRHCNCCSESCITKA------WWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIV--LVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIG--GSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYEC-----KIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
DC P+ CC +C + W RC V+H++FE FIL ++ SS TL ED L + L+ L F++ F IF +E +LK G YF W LD IV V++ F+ + F++ RT+R LRPLRA+SR GMK+V+NAL AIP I +VL+V ++FWL+FSI G+QLF GKF+KCV++ + +L S++ NK+ C + + WVN+ +NFDNV+NG+L+L Q+AT++GW ++MADAVD T+++ QP Y N Y YFV+FII G+FF LNLF+GVIIDNFN KKK S+ + +T+SQ+++ + + P RP ++++++ +F++ I+ IM N +M ++ + Q L+ + N V V IF LE +IKIIA+R +YFK W++FDF++V+ SI + VSP L+RV+RVFR+ R+LR+V+ A+ IR LL ++++S+PALFNIG LL L+MFIYAIIG+S F + ++ NFETF ++I++F++ T GWN ILD L+ + C + CG I AV YLV+Y+V FL+I+NMYIAV+L+N ++A ++ + GL++D + +Y + + G F+ L+ L EF+A + +P I P++ + ++IP+ +G + C DL+ L K L +
Sbjct: 758 DCFPE-----------CCMNACGCTSGQQHFCWLKFRCRVRCFVEHRYFEWFILFTVMVSSFTLVFEDIHLHQKPDLERALNFLNFLFAAIFSLEFILKVIGFGFVSYFTSVWNCLDALIVSVCVSVACLFENPNLSVFRSFRTVRGLRPLRAISRLGGMKVVINALFAAIPGIGSVLVVSMLFWLMFSISGVQLFAGKFYKCVNAEDEKLSPSVIPNKTACLD---HPDYRWVNANVNFDNVINGFLALLQVATYEGWMEVMADAVDSTKIDQQPSYEANLIAYAYFVVFIIVGSFFVLNLFVGVIIDNFNTLKKKYEEINSMGMLLTESQRKWVQILMAAAKRKPPSNRLRPTDCFRGKLFDLVSSNQFEVFILTVIMCNMGMMMVQHYDQ-PPLTTVIG--NYVLVGIFLLEAIIKIIAMRLHYFKKAWSIFDFVVVVSSIVGMYLGMSTESAVVSPGLLRVLRVFRVARLLRVVQFAKRIRQLLVAIIISMPALFNIGTLLFLVMFIYAIIGMSSFGNIKKQGALNDLVNFETFSSSIILIFRLGTGTGWNDILDALVVQPPH-CDPNFGGLPNGNCGFPIGAVFYLVTYIVSVFLIIVNMYIAVLLDNVNRAHENDEFGLSKDKFKSYYHTFANFSMGKQ-FLPLRVLPEFLAALDKPFKIAHPSEELIRKMKIPVREGDLVHCFDLLKVLVKKILEQ 1441 HSP 2 Score: 209.92 bits (533), Expect = 1.939e-54 Identity = 126/322 (39.13%), Postives = 189/322 (58.70%), Query Frame = 2
Query: 530 NSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENW---QNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
+S +RFS T + P +PLR I ++TH F V I++NCI + N E E IFTAIYT E I K ++RG + + YLRD WNWLD +V+ L+Y + + + LS +R+LRVLRA +T++ + G++ +V+SLL ++ L D LVL +F L + ALIGLQ++ G L +KCV ET +I NNP +W + C F+S C Y C+ NP+ G+T+FD+ G SL+ AF+++T D+WENLY ++R+ G+ +M+YF++ IF SFYL+N++L++V MSY
Sbjct: 27 SSGYQAYRFSRTASLLCLGPVHPLRMFCILLITHPYFEIIVTLVILVNCIILGLNDPPEEAEYIFTAIYTLEMICKIISRGFVKDSYAYLRDTWNWLDSLVVFLSY-LSLAPDIASLSGIRTLRVLRAFRTISALKGLRAMVNSLLRSLKLLTDVLVLFLFSLCVLALIGLQLFTGELRNKCV---------LETANATTI----------NNPSSWYHREGEPLLC-GFSST---AGRCPANYTCVAHVGPNPDHGFTNFDDIGWSLVMAFQILTMDFWENLYDRVLRSMGEWYMMYFVVGIFSCSFYLLNLVLAVVYMSY 324 HSP 3 Score: 141.739 bits (356), Expect = 1.315e-33 Identity = 89/238 (37.39%), Postives = 137/238 (57.56%), Query Frame = 2
Query: 2255 CPAYDYLKKIVSFIV---LDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQY--ERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATM 2953
CP Y L + S +V P + I I+LNTV +++ + + ++++ +N F +F E +K+ A+G Y KWN+FD +I++ S V + + S G +S+ R RLLR+ LA+SW+TM L I ++ I N+ +L +I+++FAVVGM++FG Y E F ++ IPRWNF DFTHSFM+VFR+LC E++E +WDCMRV + FFL +
Sbjct: 460 CPKYPRLSRACSALVEFIQRPLFDGIIAGLILLNTVVLTMYYHGMPDDLTFVLDILNAIFTGLFILEMIVKIFAFGLVKYICNKWNLFDSVIIITSAV----AYFLGTSTG-VSIFRILRLLRVLYLAQSWETMAKLIRAIAHSVDPIFNITCILGVIIYVFAVVGMRVFGPHYTAEAFGSR--------GIPRWNFTDFTHSFMMVFRILCSEWVEPLWDCMRVTNTAALLFFLPAL 684 HSP 4 Score: 118.242 bits (295), Expect = 1.866e-26 Identity = 134/650 (20.62%), Postives = 281/650 (43.23%), Query Frame = 2
Query: 3494 CSAF-KIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVI--------------------MCFIMLNTIVMCLESHK-------------------------QGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYS--------------------------YGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKC-GSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQ 5221
CSA + + F+ I +I+ +++ LT+ +P L +L+ ++ FT +F++EM++K FA GL KY + W D I++ + ++YF G R LR LR L W+ M ++ A+ ++ IFN+ + V +F+++GM++FG + ++ + + W NF + + ++ +F+I + W + + D + VT NT ++F+ +I G F LNLF+ +++++F +++ +L S++ Y + ++ ++ + + + Q N N +++ + I C++ V C H+ Q +L AL+ +N +F IF+LE ++K+I + YF WN D +IV + +F + ++ R R R R LR + G++ ++ +L ++P + ++ ++ ML +++I GV +F+ Y ++A NF+ + L Q++T GW ++ +++ KID++ + ++A Y V ++++ ++N+++ VI++NF+ K +
Sbjct: 470 CSALVEFIQRPLFDGIIAGLILLNTVVLTMYYHGMP--DDLTFVLDILNAIFTGLFILEMIVKIFAFGLVKYICNKWNLFDSVIIITSAVAYFLGTSTGV-SIFRILRLLRVLYLAQSWETMAKLIRAIAHSVDPIFNITCILGVIIYVFAVVGMRVFGPHY------------------TAEAFGSRGIPR--W-----NFTDFTHSFMMVFRILCSE-WVEPLWDCMRVT----------NTAALLFFLPALIVGNFIVLNLFVALLLNSFAKKEE------DLHREASEQPYVNPCSCLAHLTRTR---KTRMYYTQAVNNNKNTKRYIVEIDDCFPECCMNACGCTSGQQHFCWLKFRCRVRCFVEHRYFEWFILFTVMVSSFTLVFEDIHLHQKPDLERALNFLNFLFAAIFSLEFILKVIGFGFVSYFTSVWNCLDALIVSVCVSVA-----CLFENPNLSVFRSFRTVRGLRPLRAISRLGGMKVVINALFAAIPGIGSVLVVSMLFWLMFSISGVQLFAGKFYKCVNAEDEKLSPSVIPNKTACLDHPDYRWVNANVNFDNVINGFLALLQVATYEGWMEVMADAVDST----KIDQQPSYEANLIAYAYFVVFIIVGSFFVLNLFVGVIIDNFNTLKKKYE 1062 HSP 5 Score: 80.4925 bits (197), Expect = 6.141e-15 Identity = 50/165 (30.30%), Postives = 88/165 (53.33%), Query Frame = 2
Query: 3509 IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTK----YFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV 3988
++ H +FE + ++I+ + + L L D E + FT I+ +EM+ K + G K Y +D W WLD +V ++ +S D + IRTLR LR R +S G++ +VN+L++++ + +VL++ L + ++IG+QLF G+ KCV
Sbjct: 57 LITHPYFEIIVTLVILVNCIILGLNDP-----------PEEAEYIFTAIYTLEMICKIISRGFVKDSYAYLRDTWNWLDSLVVFLSYLSLAP--DIASLSGIRTLRVLRAFRTISALKGLRAMVNSLLRSLKLLTDVLVLFLFSLCVLALIGLQLFTGELRNKCV 208 HSP 6 Score: 78.9518 bits (193), Expect = 1.885e-14 Identity = 70/266 (26.32%), Postives = 137/266 (51.50%), Query Frame = 2
Query: 4466 INNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMF-----SEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDY 5245
I FD +I I+LNT+V+ + H +L+ LD +N +F +F LE ++KI A Y WN+FD +I+I S V + + T + + R+ R+ RVL L +S + L+ ++ S+ +FNI +L +I++++A++G+ +F +E S GI +NF F + +++F+I S + D + NT A+L+ + L++ +++N+++A++L +F++ +D+ ++ Y
Sbjct: 477 IQRPLFDGIIAGLILLNTVVLTMYYHGMPDDLTFVLDILNAIFTGLFILEMIVKIFAFGLVKYICNKWNLFDSVIIITSA--------VAYFLGTSTGVSIFRILRLLRVLYLAQSWETMAKLIRAIAHSVDPIFNITCILGVIIYVFAVVGMRVFGPHYTAEAFGSRGI-PRWNFTDFTHSFMMVFRILCSEWVEPLWDCMRVTNT--------------AALLFFLPALIVGNFIVLNLFVALLLNSFAKKEEDLHREASEQPY 719 HSP 7 Score: 67.3958 bits (163), Expect = 6.603e-11 Identity = 66/213 (30.99%), Postives = 109/213 (51.17%), Query Frame = 2
Query: 2315 ELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRL-YFSEKWNVFDFLIVVLSIVEL---TASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFF 2941
E+FI I+ N M + YD I N V + IF EA +K+IA RL YF + W++FDF++VV SIV + +++ S G L +LR FR+ R+ ++ + + + L I ++ A+ N+ +L +++FI+A++GM FG K Q N + NF F+ S +++FR+ G + D + V +C P F
Sbjct: 1115 EVFILTVIMCNMGMMMVQHYDQPPLTTVIGNYV---LVGIFLLEAIIKIIAM--RLHYFKKAWSIFDFVVVVSSIVGMYLGMSTESAVVSPGLLRVLRVFRVARLLRVVQFAKRIRQLLVAIIISMPALFNIGTLLFLVMFIYAIIGMSSFG----NIKKQGAL---NDLV---NFETFSSSIILIFRLGTGTGWNDILDALVVQPPHCDPNF 1312 HSP 8 Score: 54.299 bits (129), Expect = 5.536e-7 Identity = 44/168 (26.19%), Postives = 88/168 (52.38%), Query Frame = 2
Query: 629 HSLFSFFVLTTIIINC-------IAMTKNSESEN----VETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAY---MMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFD 1090
H F +F+L T++++ I + + + E + +F AI++ EFI+K + G + +Y WN LD +++ + ++ N L + R++R LR L+ ++ + GMK V+++L AI + LV+++ +F++ G+Q++ G +KCV D
Sbjct: 792 HRYFEWFILFTVMVSSFTLVFEDIHLHQKPDLERALNFLNFLFAAIFSLEFILKVIGFGFV----SYFTSVWNCLDALIVSVCVSVACLFENPNLSVFRSFRTVRGLRPLRAISRLGGMKVVINALFAAIPGIGSVLVVSMLFWLMFSISGVQLFAGK-FYKCVNAED 954
BLAST of Sodium channel protein vs. Ensembl Nematostella
Match: EDO46991 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RNU9]) HSP 1 Score: 266.159 bits (679), Expect = 1.649e-71 Identity = 223/794 (28.09%), Postives = 391/794 (49.24%), Query Frame = 2
Query: 3164 RRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGL----TKYFKDAWCWLDFTIVLVALISYFKVFDAGA----FKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHE---SLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPR--PKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIV----------------IFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAY--SYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQ--PNK---IRLAMLEIPICKGHTI 5416
+ HR IK W +NE + A I++++ P + P L + + ++E A EV+ ++ +P F+ + NC R +I + +F FIL++I+ SS L ED KR +L + D FT +F +E+ +K A G+ + + A+ LDF +V V+++S + D+ + + +R LR LRPLRA++R G+K VV + A+ +I N++LV ++F +F++IG+QLF G FF C D+ + E ++ K ++ W NF++V L+LF + TF+GW I+ ++D T+V+ P NN + IY+V++II AFF +N+F+G +I F + ++ EL + +K A+K +P R P + + + ++ +Q F+ +I FI NTI++ ++ + + K + LD N F +F LEC++K+ A + YF WN+FDF++V +F+ GF F +F A+ +++L+ GI+TLL++ + S AL +GLL+++ FIYA++G+ MF +A I+ NF+TF +++++LF+ +T W I+ + +C + CG+ A Y S+ I +IIN+++AVI++NF T+D + L D F W EYDP A+G ++ D+ + +I PLG + P++ RL + +P+ + T+
Sbjct: 740 KSHRLIKI---WKTNELLHL---AGKIRVSRLRKHPITDAESPSLAEDSKATLNRDHESVRSA----TSTEVM----DHEPMPPESSLFIFSNTNC------------FRVVCHRIATNSYFVNFILLLIIVSSCMLAAEDPLNSNSKR-NQVLNYFDYFFTAVFTIEITIKIIAYGVILHKGSFCRSAFNLLDFLVVAVSIVS-IALRDSSSQISVVRILRVLRVLRPLRAINRAKGLKHVVQCVFVAVKTIGNIMLVTVLFNFLFAVIGVQLFKGTFFSCTDAEKITKRECQGQYIEFKGPGLTNPVVKDREWQPQTFNFNDVPQAMLTLFTVMTFEGWPGILESSMDSTDVDEGPFLNNRPWVAIYYVIYIIIIAFFMINIFVGFVIVTFQNEGEEEFKDCEL-DKNQRKCVEFALKA-------RPTRRYIPTNRLQFHVWRVVTSQPFEYLIFAFITGNTILLMMQYYNEPKLYTRVLDGFNIGFTSVFLLECILKLFAFKPKNYFLDRWNLFDFVVVVGSVVDITMNEVSSEQMFAFGF-----FRLFRALR--------------LVKLLNQGSGIKTLLWTFIKSFQALPYVGLLIIMTFFIYAVVGMQMFGRIAIDPETQINRNNNFQTFPQSLMVLFRSATGENWQLIMLACTDTPNAKCDPNAYPQDTDGLCGTD-FAYAYFCSFYAICSFLIINLFVAVIMDNFDYLTRDWSI-LGPHHLDEFVRVWSEYDPEASGCVKHVDIVTVLKRIAPPLGFGKFCPHREACKRLVSMNMPLNRDGTV 1476 HSP 2 Score: 149.058 bits (375), Expect = 8.429e-36 Identity = 171/649 (26.35%), Postives = 311/649 (47.92%), Query Frame = 2
Query: 3527 FETFILIMIVASSLTLTLEDKFLPTRKR----LKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYF-KVFDAGAF--KAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFK-CVDSNNVRLHESLVQ-----NKSDCYE----------LQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRY----YHAM---------------------------------------------KKMSSISPQKPVPRPKSTIAQIFYNI-------INNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVS--PTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGID--ATFNFETFGKAMIILFQISTSGGWNSIL-DGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQA 5206
F+ ILI I A+ L F P ++ + + LE + F IF +E VLK A G Y ++ W LDF IV+V L + K++ +F KA+R R LRPLR VS +++V+N++I+A+ +F++ L+ + +I++IIG++LF GK C D NV + + +S+ Y L+K E ++ NFDN+ L++FQ T +GWTD+M D N++ ++YFV II+G+FF LNL +GV+ F +K + S E F +K+ YH ++ S+ S Q + R K + + + + + Q F ++ F+ LN++ + LE + Q + L+ LD NK+F+ +F LE V+K+ L ++ YF +N FD ++VI S + + + A+ P I ++R R+ R+ ++ + + L+ SL+ S+ ++ + LLL L M I++++G+ +F ++ G + NF++F +A++ +FQI T WN+++ G+ + + + +LY + +V+ +++N+++A+ ++N + A
Sbjct: 76 FDVMILITIFANCAALA---AFQPLPEQDSSLINEELEVAEFVFLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIVKLYTPDSFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKALIPLFHIALLVVFVVIIYAIIGVELFMGKLHSTCYD--NVTGQPTFDEPHPCSTESEGYSCSNAGPGQVCLKKWEGPNY--GITNFDNIGLACLTVFQCITLEGWTDVMYSINDAI---------GNSWPWLYFVTLIIWGSFFVLNLVLGVLSGEFAKEKARAQKSGE-FQKLREKQLVDDAYHGYLDWISQAEDIEGDSSAGEDEEGKADRKPSFRRRKENDDISKNKENQEDSAASDQGWIDRKKKILKRFHHRLRRSCRKAVKTQWFYWTVIVFVFLNSLTLALEHYNQPEFLTQFLDKANKLFLALFTLEMVVKMYCLGFHGYFASLFNRFDCLVVISS-----LLELGLTEAMDQRPIGISMLRCVRLLRIFKVTRYWSSLSNLVASLLNSMRSIMGLLLLLSLFMVIFSLLGMQIFGG-KFNLGDEDVPRSNFDSFWRALVTVFQILTGEDWNAVMYTGIQSWGGITESLSA-------IPILYFIFLVVVGNYILLNVFLAIAVDNLADA 694 HSP 3 Score: 120.168 bits (300), Expect = 5.667e-27 Identity = 95/332 (28.61%), Postives = 172/332 (51.81%), Query Frame = 2
Query: 566 KAFFLFSPFNPLRAAAIRILT-------HSLFSFFVLTTIIINCIAMTK------------NSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDF--IVIGLAYM---MYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
+A F + NP+R+ AI + T + F +L TI NC A+ N E E E +F I+T E ++K +A G +++ YLR+ WN LDF +V+GLA + +Y + + ALR+ RVLR L+ V+ +P ++ V++S++ A+I L +L VF++ I+A+IG++++ G L C +DN + G T++ +P + ++ C N V C +++ E PN+G T+FDN G++ L F+ +T + W + +Y + +YF+ +I +GSF+++N++L +++ +
Sbjct: 45 RALFCLTLGNPIRSTAISLFTVFFLDVFRTPFDVMILITIFANCAALAAFQPLPEQDSSLINEELEVAEFVFLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIVKLYTPDSF-DVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKALIPLFHIALLVVFVVIIYAIIGVELFMGKLHSTC---YDNVT-GQPTFDEP-------------HPCSTESEGYSCSNAGPGQV----CLKKW-------EGPNYGITNFDNIGLACLTVFQCITLEGWTDVMYSINDAIGNSWPWLYFVTLIIWGSFFVLNLVLGVLSGEF 347 HSP 4 Score: 87.4261 bits (215), Expect = 5.382e-17 Identity = 58/200 (29.00%), Postives = 112/200 (56.00%), Query Frame = 2
Query: 2324 ITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGI 2923
+ + + LN++ ++++ Y+ + ++K N F+ +FT E +K+ G YF+ +N FD L+V+ S++EL ++ + +S+LR RLLRIFK+ + W +++ L + ++ +I L ++L++ + IF+++GMQIFG +F + +PR NF F + + VF++L GE W+ + GI
Sbjct: 464 VIVFVFLNSLTLALEHYNQPEFLTQFLDKANKLFLALFTLEMVVKMYCLGFHGYFASLFNRFDCLVVISSLLELGLTEAMDQRPIGISMLRCVRLLRIFKVTRYWSSLSNLVASLLNSMRSIMGLLLLLSLFMVIFSLLGMQIFG---------GKFNLGDEDVPRSNFDSFWRALVTVFQILTGED----WNAVMYTGI 650 HSP 5 Score: 73.559 bits (179), Expect = 9.980e-13 Identity = 58/178 (32.58%), Postives = 103/178 (57.87%), Query Frame = 2
Query: 569 AFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIIN-CI-----AMTKNSESENV----ETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYV----NSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGT 1060
+ F+FS N R RI T+S F F+L II++ C+ + NS+ V + FTA++T E IK +A G+I+++ ++ R A+N LDF+V+ ++ + +S + + LR LRVLR L+ + G+K VV + +A+ + + +++TV +FA+IG+Q+++GT
Sbjct: 812 SLFIFSNTNCFRVVCHRIATNSYFVNFILLLIIVSSCMLAAEDPLNSNSKRNQVLNYFDYFFTAVFTIEITIKIIAYGVILHKGSFCRSAFNLLDFLVVAVSIVSIALRDSSSQISVVRILRVLRVLRPLRAINRAKGLKHVVQCVFVAVKTIGNIMLVTVLFNFLFAVIGVQLFKGT 989 HSP 6 Score: 72.0182 bits (175), Expect = 2.278e-12 Identity = 64/215 (29.77%), Postives = 106/215 (49.30%), Query Frame = 2
Query: 2294 IVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGE-YMESMWDCMRVNGIYCIP 2935
+ PF E I I NT+ + + Y+ +++ N+ F +F E +KL A+ P+ YF ++WN+FDF++VV S+V++T ++ S R FR LR+ KL + L ++ A+ + +++ + FI+AVVGMQ+FGR +T+ ++N NF F S M++FR GE + M C C P
Sbjct: 1151 VTSQPF-EYLIFAFITGNTILLMMQYYNEPKLYTRVLDGFNIGFTSVFLLECILKLFAFKPKNYFLDRWNLFDFVVVVGSVVDITMNEVSSEQMFAFGFFRLFRALRLVKLLNQGSGIKTLLWTFIKSFQALPYVGLLIIMTFFIYAVVGMQMFGRIA--IDPETQINRNN------NFQTFPQSLMVLFRSATGENWQLIMLACTDTPNAKCDP 1356 HSP 7 Score: 71.633 bits (174), Expect = 3.299e-12 Identity = 73/282 (25.89%), Postives = 132/282 (46.81%), Query Frame = 2
Query: 3488 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALI--SYFKVFDAG--AFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVD----VTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNF 4309
+R S K V ++F +++ + +SLTL LE P + L L+ + F +F +EMV+K + +G YF + D +V+ +L+ + D +R +R LR + W + +V +L+ ++ SI +LL+ +F +IFS++GMQ+FGGKF N D E+V + NFD+ +++FQI T + W +M + +TE + + +YF+ ++ G + LN+F+ + +DN
Sbjct: 447 LRRSCRKAVKTQWFYWTVIVFVFLNSLTLALEHYNQP--EFLTQFLDKANKLFLALFTLEMVVKMYCLGFHGYFASLFNRFDCLVVISSLLELGLTEAMDQRPIGISMLRCVRLLRIFKVTRYWSSLSNLVASLLNSMRSIMGLLLLLSLFMVIFSLLGMQIFGGKF-----------------NLGD-------EDV----PRSNFDSFWRALVTVFQILTGEDWNAVMYTGIQSWGGITE-------SLSAIPILYFIFLVVVGNYILLNVFLAIAVDNL 691 HSP 8 Score: 53.9138 bits (128), Expect = 8.068e-7 Identity = 51/207 (24.64%), Postives = 93/207 (44.93%), Query Frame = 2
Query: 4418 PVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCL---ESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-----YFKIPWNVFDFIIVIFSIGFWVIEKFV----------IFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETF 4984
P+ ++ +F+ + FD++I+ I N + + +++ L+ VF+ IF +E V+KIIA + Y + WN+ DF+IV+ + +++ + F + P LRLV ++ +L S++ +L LF+I LL++ ++ IYAIIGV +F +S D TF
Sbjct: 55 PIRSTAISLFTVFFLDVFRTPFDVMILITIFANCAALAAFQPLPEQDSSLINEELEVAEFVFLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIVKLYTPDSFDVKALRAFRVLRP--------------LRLVSGVPSLQVVLNSIIKALIPLFHIALLVVFVVIIYAIIGVELFMGKLHSTCYDNVTGQPTF 247 HSP 9 Score: 53.1434 bits (126), Expect = 1.371e-6 Identity = 43/153 (28.10%), Postives = 82/153 (53.59%), Query Frame = 2
Query: 2309 FMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFS----EKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIF 2755
F+ + + IV + + + D +++ ++N + FF +FT E +K+IAYG L+ +N+ DFL+V +SIV + A +D S+ + +LR R+LR + + + + + + IGN+ +V + F+FAV+G+Q+F
Sbjct: 835 FVNFILLLIIVSSCMLAAEDPLNSNSKRNQVLNYFDYFFTAVFTIEITIKIIAYGVILHKGSFCRSAFNLLDFLVVAVSIVSI-ALRDSSSQISVVRILRVLRVLRPLRAINRAKGLKHVVQCVFVAVKTIGNIMLVTVLFNFLFAVIGVQLF 986 HSP 10 Score: 50.8322 bits (120), Expect = 7.316e-6 Identity = 29/90 (32.22%), Postives = 48/90 (53.33%), Query Frame = 2
Query: 2423 FMVIFTFEAGMKLIAYG----PRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTL 2680
F+ IFT E+ +K+IAYG P Y WN+ DF+IVV+ + + K + ++ LR FR+LR +L ++ ++ I + L
Sbjct: 116 FLGIFTMESVLKIIAYGFVMHPGAYLRNGWNILDFVIVVVGLATIIV-KLYTPDSFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAL 204
BLAST of Sodium channel protein vs. Ensembl Nematostella
Match: EDO36844 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHI0]) HSP 1 Score: 253.832 bits (647), Expect = 8.763e-68 Identity = 187/578 (32.35%), Postives = 311/578 (53.81%), Query Frame = 2
Query: 3446 NCCSESCITKAWWWIRCSAFKIVD-----HKFFETFILIMIVASSLTLTLED-KFLPT--RKRLKDILEFMDLGFTVIFLVEMVLKWFAIGL----TKYFKDAWCWLDFTIVLVALISYFKVFDA-------GAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMS---SISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALR-WYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVA------YSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKK 5092
+CCS+ + + + F++++ +K+F+ +L+ I+ + + + LE LP +R+ DI ++ LG IF +EM++K A+GL Y + W +D +V+++ + G + R LR LRPLR +SR G+KIVV LI ++ I N++L+ F++IF I+G+QLF GKF C D+ V K++C E SWVN + NFDN+ L+LF +T GW +IM D +D ++ QP N+ + IYFV F++ F LN+ +GV+++NF + I ++ K+ ++++ ++S P PR + + + + FD+ I I LN + M LE ++Q L+ L N VF +F LE ++KI AL Y K WN D +IVI SI +E+ + ++PT+IRV+RV RI RVL+L+K+A GIR LL ++ +LP + N+G+L +L+ FI++ +G+ +F ++ G+D NF +FG AM+ LF+IST WN IL ++ + C+++ +
Sbjct: 901 SCCSKRADWSLYVFAPDNRFRMLNKELYQNKWFDRTVLLFILLNCVVMALEGPSVLPGSLERRVIDICMYVFLG---IFTIEMMVKVIALGLWIGPDAYLRSGWNVMDGFLVVISWVDVIVTATTNGQNSILGVLRVFRALRTLRPLRVISRAPGLKIVVETLISSLKPIGNIVLIAATFFIIFGILGVQLFKGKFHYCTDAT------VPVTTKTECLE----NGGSWVNREYNFDNLAKALLTLFVFSTKDGWVNIMYDGIDAVGIDKQPIRNHARYNVIYFVGFLLLAGFVVLNMLVGVVVENFQKCRTLIEMEKKIDEEKKNKKEEKMRREIAEDEALSEHYPRPR------KFIHAVCTHGYFDLGIAAVIALNVLCMALEHYQQPDGLTAFLKCANYVFTAVFILEAILKIFALGIKRYIKDRWNQLDMLIVILSIVGIALEEMTMELPINPTIIRVMRVLRIARVLKLLKTAEGIRKLLDTVAQALPQVGNLGMLFLLMFFIFSALGIELFGKIDCEKPGITCQGMDEHANFRSFGIAMLTLFRISTGDNWNGILKDTISPDV--CRVNPE 1457 HSP 2 Score: 93.2041 bits (230), Expect = 9.640e-19 Identity = 66/237 (27.85%), Postives = 114/237 (48.10%), Query Frame = 2
Query: 3491 RCSAFK-----IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVT 4186
RC+ F+ VD K+F I+ I ++LT+ +E P +++ D+LE + FT IF +EM++K +G Y KDA+ D TIV+++++ F D+G +R+ R LR + V ++ + +I + ++ L + ++F SI+GM LFGGK Y E V S+ NFD++ +++FQ+ T + W +M D + T
Sbjct: 396 RCAKFRDGCASSVDSKWFMYVIMGAIFVNTLTMGIEYYGQP--QKMTDVLEIFNYIFTAIFGIEMIMKLIGLGFYGYIKDAFNIFDGTIVIISVVELFGDDDSG-ISVLRSFRLLRVFKLVRFLPALRRQLLVMIHTMDNVMTFLALLVIFMFTASILGMNLFGGK-----------------------YRFPNDEGV-METSRANFDDLFWAIVTVFQVLTQEDWNIVMYDGMRAT 605 HSP 3 Score: 89.7373 bits (221), Expect = 1.187e-17 Identity = 82/279 (29.39%), Postives = 136/279 (48.75%), Query Frame = 2
Query: 4469 NNQKFDIVIMCFIMLNTIVMCLESHK--QGKELSDALDHMNKVFVLIFALECVIKIIALRWY-----YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSAR------GIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDAT--------------------FNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYI-LAVLYLVSYLVISFLVIINMYIAVILENFSQ 5203
N+ FD ++ FI+LN +VM LE G +D VF+ IF +E ++K+IAL + Y + WNV D +V+ S WV VI A + ++ V R+ R LR ++ R G++ ++ +L+ SL + NI L+ I+ I+GV +F + + Y DAT +NF+ KA++ LF ST GW +I M + IDK+ ++ V+Y V +L+++ V++NM + V++ENF +
Sbjct: 929 QNKWFDRTVLLFILLNCVVMALEGPSVLPGSLERRVIDICMYVFLGIFTIEMMVKVIALGLWIGPDAYLRSGWNVMDGFLVVIS---WVD---VIVTATTNGQNSILGVLRVFRALRTLRPLRVISRAPGLKIVVETLISSLKPIGNIVLIAATFFIIFGILGVQLF-KGKFHYCTDATVPVTTKTECLENGGSWVNREYNFDNLAKALLTLFVFSTKDGWVNI----MYDGIDAVGIDKQPIRNHARYNVIYFVGFLLLAGFVVLNMLVGVVVENFQK 1196 HSP 4 Score: 86.6557 bits (213), Expect = 8.240e-17 Identity = 67/210 (31.90%), Postives = 104/210 (49.52%), Query Frame = 2
Query: 2333 CIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNG--KIPRWNFCDFTHSFMIVFRVLCGE-YMESMWDCMRVNGIYCIPFFLATM 2953
I +NT+ M I+ Y M ++ N F IF E MKLI G Y + +N+FD IV++S+VEL D +S+LR+FRLLR+FKL + + ++ T+ + +L I +F +++GM +FG +Y FP D G + R NF D + + VF+VL E + M+D MR + +F+ M
Sbjct: 420 AIFVNTLTMGIEYYGQPQKMTDVLEIFNYIFTAIFGIEMIMKLIGLGFYGYIKDAFNIFDGTIVIISVVELFGDDD-----SGISVLRSFRLLRVFKLVRFLPALRRQLLVMIHTMDNVMTFLALLVIFMFTASILGMNLFGGKY-------RFPNDEGVMETSRANFDDLFWAIVTVFQVLTQEDWNIVMYDGMRATSKWAGLYFILLM 617 HSP 5 Score: 78.1814 bits (191), Expect = 3.857e-14 Identity = 55/211 (26.07%), Postives = 103/211 (48.82%), Query Frame = 2
Query: 2264 YDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASK---DISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFP--KDNGKIPRWNFCDFTHSFMIVFRVLCGE 2881
Y +K + + + +L I I LN + M+++ Y + + N F +F EA +K+ A G + Y ++WN D LIV+LSIV + + ++ + + ++R R+ R+ KL K+ + + L + + L +GNL ++ ++ FIF+ +G+++FG K E P G NF F + + +FR+ G+
Sbjct: 1233 YPRPRKFIHAVCTHGYFDLGIAAVIALNVLCMALEHYQQPDGLTAFLKCANYVFTAVFILEAILKIFALGIKRYIKDRWNQLDMLIVILSIVGIALEEMTMELPINPTIIRVMRVLRIARVLKLLKTAEGIRKLLDTVAQALPQVGNLGMLFLLMFFIFSALGIELFG------KIDCEKPGITCQGMDEHANFRSFGIAMLTLFRISTGD 1437 HSP 6 Score: 77.0258 bits (188), Expect = 8.803e-14 Identity = 67/278 (24.10%), Postives = 130/278 (46.76%), Query Frame = 2
Query: 4466 INNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSI---------GFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGID------ATFNFETFGKAMIILFQISTSGGWNSIL-DGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYD 5248
++++ F VIM I +NT+ M +E + Q ++++D L+ N +F IF +E ++K+I L +Y Y K +N+FD IVI S+ G V+ F + LV+ +R L ++ ++ + LL++ MF +I+G+++F Y + D + NF+ A++ +FQ+ T WN ++ DG+ + + A LY + + I ++ N+ +A+++E F+ A ++ +YD
Sbjct: 408 VDSKWFMYVIMGAIFVNTLTMGIEYYGQPQKMTDVLEIFNYIFTAIFGIEMIMKLIGLGFYGYIKDAFNIFDGTIVIISVVELFGDDDSGISVLRSFRLLRVFK-----------------LVRFLPALRRQLLVMIHTMDNVMTFLALLVIFMFTASILGMNLFGG-KYRFPNDEGVMETSRANFDDLFWAIVTVFQVLTQEDWNIVMYDGMRATSKW--------------AGLYFILLMTIGNYILFNLLVAILVEGFASAPAGYD---SEGNYD 650 HSP 7 Score: 76.6406 bits (187), Expect = 9.657e-14 Identity = 82/337 (24.33%), Postives = 141/337 (41.84%), Query Frame = 2
Query: 569 AFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSES---ENVET--------IFTAIYTAEFIIKALARGLIVYQFTYLRDAWN----------WLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWEN-LYQLI---------IRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
+ ++F+P N R + + F VL I++NC+ M S ++E +F I+T E ++K +A GL + YLR WN W+D IV A NS LG L R+LR LR L+ ++ PG+K VV +L+ ++ + + +++ IF ++G+Q+++G H C D+ + K + N +W N EY +FDN +LL F T+D W N +Y I IR + ++IYF+ + F ++N+++ +V ++
Sbjct: 910 SLYVFAPDNRFRMLNKELYQNKWFDRTVLLFILLNCVVMALEGPSVLPGSLERRVIDICMYVFLGIFTIEMMVKVIALGLWIGPDAYLRSGWNVMDGFLVVISWVDVIVT--ATTNGQNSILGVLRVFRALRTLRPLRVISRAPGLKIVVETLISSLKPIGNIVLIAATFFIIFGILGVQLFKGK-FHYCT---------------DATVPVTTKTECLENGGSW-------------------VNREY---------------NFDNLAKALLTLFVFSTKDGWVNIMYDGIDAVGIDKQPIRNHARYNVIYFVGFLLLAGFVVLNMLVGVVVENF 1194 HSP 8 Score: 60.8474 bits (146), Expect = 6.423e-9 Identity = 49/219 (22.37%), Postives = 97/219 (44.29%), Query Frame = 2
Query: 917 KTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNN---------PENWQN--------PITFCHN---------FTSNNVIKSTC---NEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
+ + +P ++ +V+ LL + L + L + + SIF ++G+QM++G L +CV A+ N S NI Y PEN + P + H +++ + + C N+ Y NP + SFDN ++ + F+++T + W ++ I +G + IYF+++I GS+++ N+ L ++ +
Sbjct: 14 RAINRVPSIRILVTLLLDTLPMLGNVLAMCSLIFSIFGIVGVQMWQGVLRSRCV---------AQLPGNLSANQLNISAFYQPGGSSDLVCSLPENSGDKHCTSAFIPASTAHGRECGLTYEQYSNRSNYTNVCANWNQYYTLCADMGINPLYDAVSFDNILIAWVAIFQVITLEGWTDIMYYIQDAHGFWNFIYFVVLIVIGSYFMTNLCLVVITTQF 223 HSP 9 Score: 58.9214 bits (141), Expect = 2.642e-8 Identity = 47/184 (25.54%), Postives = 81/184 (44.02%), Query Frame = 2
Query: 3797 AIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF-FKCV-------DSNNVRLHE------------SLVQNKSD--------------------CYELQKTENVSWVNSQINFD---------------------NVLNGYLSLFQIATFKGWTDIM 4165
A+R +R LRPLRA++R ++I+V L+ +P + NVL +C + + IF I+G+Q++ G +CV +N + + SL +N D YE Q + ++ N N++ N+L ++++FQ+ T +GWTDIM
Sbjct: 3 AVRAIRVLRPLRAINRVPSIRILVTLLLDTLPMLGNVLAMCSLIFSIFGIVGVQMWQGVLRSRCVAQLPGNLSANQLNISAFYQPGGSSDLVCSLPENSGDKHCTSAFIPASTAHGRECGLTYE-QYSNRSNYTNVCANWNQYYTLCADMGINPLYDAVSFDNILIAWVAIFQVITLEGWTDIM 185
BLAST of Sodium channel protein vs. Ensembl Nematostella
Match: EDO35497 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLD1]) HSP 1 Score: 214.157 bits (544), Expect = 2.675e-59 Identity = 174/530 (32.83%), Postives = 288/530 (54.34%), Query Frame = 2
Query: 3509 IVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDA-------GAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-YFKIPWNVFDFIIVIFSIGFWVIEKFV-IFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSY-----GIDATFNFETFGKAMIILFQISTSGGWNSIL 5044
+ H+ F+ IL+ I+ S L +E + + + I++ L FT+IF +EM++K A+GL Y +D W LD +V+V+ I + + G + R LR LRPLR + R G+K+VV L+ ++ I N +L+ +F+++F I+G+QLF GKF+ C +S V +K +C + + WVN + NFD++L +SLF ++T GW +IM +D +V+ QP N + +YF+ F++ G F LN+ +GV+++NF ++++ + Q R + KK + + + I ++ +DI I I +N I M LE +K + ++ N F +F +E V+K+IAL + Y K WN+ D IV+ S+ V+E V + + +PT+IR +RV RI RVL+LVK A+G+R+LL +L +LP + N+GLL L+ FIY+ +G+ +F + S+ G + +F FG AM+ LF+I+T WN IL
Sbjct: 15 VCSHRAFDYVILVFIIFSCAVLAIEAPDIAEQGLKRQIIDISMLVFTIIFTIEMLIKLVAMGLVLGPGTYLRDGWDVLDGFLVMVSWIDIIVTYTSHVSPEVLGTLRVFRALRTLRPLRVIRRAPGLKLVVQTLLYSLKPIGNTVLIAAIFFVMFGILGVQLFKGKFYYCEG-------DSHVISKQECANSTRGQ---WVNRRYNFDDLLQALISLFVVSTKDGWVEIMHHGIDAVDVDVQPIVNYAEWRLVYFIPFLLLGGFLVLNMIVGVVVENFQRCRERMD-------EEEQARPHRKGKKARNQMQDSLYYESYGPLRLKIHFICTHRNWDITIAAIICINVICMSLEHYKMPQVF---VETTNYFFTSVFVIEVVVKVIALGFVRYPKDRWNLIDLAIVLLSVTGIVLELLVKVDHLFNPTVIRTLRVLRITRVLKLVKLAKGVRSLLDTLFEALPQVPNLGLLFFLLFFIYSCLGIQLFGSLECSHDYPCQGFNRHAHFRDFGTAMLTLFRIATGDNWNGIL 524 HSP 2 Score: 64.6994 bits (156), Expect = 2.494e-10 Identity = 49/168 (29.17%), Postives = 85/168 (50.60%), Query Frame = 2
Query: 620 ILTHSLFSFFVLTTIIINCIAMT-----------KNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWN----------WLDFIVIGLAYMMYVN-SALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRG 1057
+ +H F + +L II +C + K + +FT I+T E +IK +A GL++ TYLRD W+ W+D IV Y +V+ LG L R+LR LR L+ + PG+K VV +LL ++ + + +++ +F ++G+Q+++G
Sbjct: 15 VCSHRAFDYVILVFIIFSCAVLAIEAPDIAEQGLKRQIIDISMLVFTIIFTIEMLIKLVAMGLVLGPGTYLRDGWDVLDGFLVMVSWIDIIV---TYTSHVSPEVLGTLRVFRALRTLRPLRVIRRAPGLKLVVQTLLYSLKPIGNTVLIAAIFFVMFGILGVQLFKG 179 HSP 3 Score: 62.3882 bits (150), Expect = 1.151e-9 Identity = 71/278 (25.54%), Postives = 140/278 (50.36%), Query Frame = 2
Query: 4463 IINNQKFDIVIMCFIMLNTIVMCLES---HKQGKELSDALDHMNKVFVLIFALECVIKIIALRWY-----YFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSAR---GIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMF-SEVAYSYG------------------IDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYI-LAVLYLVSYLVISFLVIINMYIAVILENFSQ 5203
+ +++ FD VI+ FI+ + V+ +E+ +QG + +D VF +IF +E +IK++A+ Y + W+V D +V+ S W+ + VSP ++ +RVFR R LR ++ R G++ ++ +L+ SL + N L+ + ++ I+GV +F + Y G ++ +NF+ +A+I LF +ST GW I M++ +D + +Y ++Y + +L++ +++NM + V++ENF +
Sbjct: 15 VCSHRAFDYVILVFIIFSCAVLAIEAPDIAEQGLK-RQIIDISMLVFTIIFTIEMLIKLVAMGLVLGPGTYLRDGWDVLDGFLVMVS---WIDIIVTYTSHVSPEVLGTLRVFRALRTLRPLRVIRRAPGLKLVVQTLLYSLKPIGNTVLIAAIFFVMFGILGVQLFKGKFYYCEGDSHVISKQECANSTRGQWVNRRYNFDDLLQALISLFVVSTKDGWVEI----MHHGIDAVDVDVQPIVNYAEWRLVYFIPFLLLGGFLVLNMIVGVVVENFQR 284
BLAST of Sodium channel protein vs. Ensembl Medaka
Match: ENSORLT00000043679.1 (sodium channel protein type 2 subunit alpha [Source:NCBI gene;Acc:101155239]) HSP 1 Score: 1103.2 bits (2852), Expect = 0.000e+0 Identity = 759/1823 (41.63%), Postives = 1070/1823 (58.69%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINC----------IAMTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIR--------------IQNLPKSHVSLYLPRTKSLQEISKLNVDQ---KSDIAESKHKES--NDNMLIDSCPSLHRTNRFKVYQQF------NVFR----KLIEDNRKESSKYS--LDDKRFSDLASCQ------------------VNTSKAQHD--SLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISAL------SVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTY---DNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIY-CIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYN---HIGKKLMKIIRRHRQIKYPFPWDS---------NESNPINIQAA-------IIKMNKNET------EPSRNVHDPQLNQVAFNLWLENYEPKFDADPD----------INE-----------AEVIFWNQPYDCLP----DSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD-SNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
++ L AGK LP GD LE+LDP Y +++TF+V+N K IFRF++ A + PFNP+R +I+IL HS + L + I+C + ++ ++ VE FTAIYT E ++K LARG + +FT+LRD WNWLDF VI LAY+ + G+LSALR+ RVLRA K ++ IPG+KT+V +L ++ KL D ++LTVF LS+FAL+GLQ++ G L +KCV+ ++ + + L W + +P+ N+ C E Y C+ + P+FGYTSFD+FG + L FRLMTQD+WENLYQ +R +G +MI+F++VIF GSFYLIN+IL++VAM+Y++ ++ E +E+E E + E+ +Q E + A S D G N+ R + +PKS+ L + EIS L D SD+ S +S N + S +T+ F Q N F + E+NR S S L D+ S L+ C N +Q D S + LS ++K S+ N + R N D L + + PE ++R +L +V E E +K +F + F W+CCPA+ +KK V IV+DPF +L ITICIVLNT+FM+++ + D M+ I NKV F IFT E +K+IA P YF E+WN+FD +IV +S+VEL S ++LLR+ RLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+F R+Y N ++ D + PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G+ C+ ++ M++GNLV + F + + ++ +E +D+N LQ A+ R+ R ++K L + N H KK + + K PF + + ++ +N+ I+ N++ PS V P + V + + N E F +D D IN+ E+ N P C P +I+C C + KAWW +R + F+IV+H +FE+FI+ MI+ SS L ED + RK +K ILEF D FT IF++EM+LKW A G KYF +AWCWLDF IV V+L+S ++ D GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSIFNVLLVCL+FWLIFSI+G+ LF GKF+KC++ ++ V + NK+ C ++ W N +IN+DNV GYL+L Q+ATFKGW DIM AVD E + QP + N Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKKI S +LFMT+ QK+YY+AMKK+ S PQKP+PRP + I ++II+ Q FDIVIM I LN + M +E+ Q ++ + L +N VF++IF+ EC++K+I LR Y+F WN+FD ++VI SI + K + VSPTL RVIR+ RIGRVLRL+KSA+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TSGGW+++LD ++N+N +C +K+ CG+ + + + VSY++ISFL+++NMYIAVILENF+ AT++ L++DD+++FYE WE +DPGAT F+ LS+F + PL +P+PN+I L +++PI G I C+D++ TK LGE
Sbjct: 52 NRDLMAGKPLPILYGDI--PNGLVSTPLEELDPAYYNQKTFIVLNKRKMIFRFNAAPALYFLGPFNPIRRLSIKILVHS----YPLHKVDIDCELIWFKMGHTLRNCNSTWTKPVEYTFTAIYTFESLVKILARGFCLGEFTFLRDPWNWLDFSVIVLAYLNEF-ADFGRLSALRTFRVLRAFKAISVIPGLKTIVGALFQSVKKLTDVMILTVFCLSVFALVGLQLFMGHLKNKCVRQPEHVTPEENLTDRKYFLQWLL------------DPL-----LCGNSTYAGKCPEGYTCV-SFGSTPDFGYTSFDSFGWAFLALFRLMTQDFWENLYQQTLRASGKPYMIFFVLVIFLGSFYLINLILAVVAMAYEEQSQTNIKEAKEKEEEFQAMLEELKQQQEEVQVSNTAESKDPGDKGDG---TGGTSNLTSKSAKERCNMKKKKKEEERKEVSEIPKSNSLNSLKK-----EISHLTTDGNLLNSDLKSSTTHQSFLNFRGALISSRRYSKTSLFSFQSQIQDLSSENEFADDEYSIFEENRSGSGSMSHHLHDRHSSSLSECLFLPPLLLQPDGLKHSSIGCNGCMSQKDRSSPPVGFLLSKVALDKTFSQEN-VRNALRFPNSSYSD---LMDYLEGPEARQRAQSLVSVITSTVEELEESRKKCPPCWYKFAENFLIWDCCPAWQKIKKFVKLIVMDPFADLTITICIVLNTLFMAMEHHPMQDEFTKMLSIGNKV---FTGIFTAEMVLKIIALDPYYYFQERWNIFDGIIVTISLVELCFS---------INLLRSLRLLRVFKLAKSWPTLNSLLKIIGNSVGAMGNLTLVLAIIVFIFAVVGMQLFEREYRY--NVSKISAD--RKPRWHMMDFFHSFLIVFRVLCGEWIETMWDCMEVAGVTKCLIVYMMVMVVGNLVFTSCFHSFSADNLAAI------EEDSDLNNLQIAIKRICRGFDFIKSFLKNICNSNCHKEKKEKTKDKSLGEFKKPFGTNDLHNHTLKNFHTNSNVNLTGVDKSGDKYIVNSKSNDSIMSFINNPSLTVTVP-IAAVESDFEVLNTE-DFSSDEDEELSSSEGSTINDYEAGEIADSVGGELEESNDPSPCFPAVCLQNIKC-----CQVNVDVGFWKAWWKLRQTCFRIVEHSWFESFIIFMILLSSGALAFEDVYSEQRKTIKIILEFADKMFTYIFILEMLLKWLAYGFAKYFTNAWCWLDFLIVDVSLVSLVANALELTDLGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIFNVLLVCLIFWLIFSIMGVNLFAGKFYKCINKTDGVECDPENIPNKTFC---TNETDLIWKNLKINYDNVGMGYLALLQVATFKGWMDIMYAAVDSGEPDEQPVFERNIKMYLYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKI--STDLFMTEDQKKYYNAMKKLGSKKPQKPIPRPSNKIQGFIFDIISKQAFDIVIMVLIWLNMVTMMVETADQSEKQTYILRVINYVFIVIFSGECLLKMIGLRHYFFMNGWNIFDLVVVILSIAGVFLAKIIEDYFVSPTLFRVIRLARIGRVLRLIKSAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSSFAYVKKDKGIDDLFNFETFGNSMICLFQITTSGGWDTLLDPILNSNIDDCDRNKEHPGSNTKGDCGNPPVGIAFFVSYIIISFLIVVNMYIAVILENFNVATEESTDPLSEDDFEMFYEVWERFDPGATQFIHYYSLSDFADALNPPLQVPKPNRIDLIFMDLPIVSGDRIHCLDILFAFTKRVLGE 1803
BLAST of Sodium channel protein vs. Ensembl Medaka
Match: scn12aa (sodium channel protein type 4 subunit alpha [Source:NCBI gene;Acc:101159774]) HSP 1 Score: 874.389 bits (2258), Expect = 0.000e+0 Identity = 539/1125 (47.91%), Postives = 749/1125 (66.58%), Query Frame = 2
Query: 2225 FNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHI--GKK--------LMKIIRRHRQI-------KYPFP-WDSNESNPINIQAAIIKMNKNETE---------PSRNVHDPQLNQVAFNLWLENYEPKFDAD--PDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
F K + W CCP + LK+ V F+V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +KL A P YF + WN+FD +IV LS++EL +S +G LS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + + F + ++PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G CI F+ M++GNLV+LNLFLALLLSSFSS++L DE D+N +Q A+ R+ I W+ + +N G+K +K+ H + KY P DS +NP I +++ E S + + ++ ++ +P +AD +++E + +P DC P F +RHC CC+ + + AWW +R + ++IV+H +FETFI+ MI+ SS L ED ++ RK +K ILE+ D F+ IF++EM LKW A G KYF + WCWLDF IV V+LIS D A K++RTLRALRPLRA+SR++GM++VVNALI AIPSI NVLLVCL+FWLIFSI+G+ LF GKF KCV+ ++V NKSDC + T+ W ++NFDNV GYLSL Q+ATFKGW +IM AVD V QP N + YIYFV+FIIFG+FFTLNLFIGVIIDNFN QK+K+GG +LFMT+ QK+YY+AMKK+ S PQKP+PRP + + F+++++ Q FDI+IM I++N + M +E+ +Q +++ L+ +N VF++IF EC+IKI ALR Y+F + WN+FDF+++I SI V+ + VSPTL RVIR+ RIGRVLRL+++A+GIRTLLF+L++S+PALFNIGLLL L+MFIYAI G++ F+ V GID FNFETFG ++I LFQISTS GW+++L +M+N+ EC I + CG+ + + + VSY++ISFL+++NMYIA+ILENFS AT++ L++DD+++FYE WE++D AT F+E LS F + EPL I +PNKI L +++P+ G I C+D++ TK LGE
Sbjct: 600 FAKRYLIWSCCPWWLKLKEWVKFMVMDPFLDLGITICIVLNTLFMALEHYPMTDEFNTMLSVGNLVFTGIFTAEMVLKLTALDPYYYFQQGWNIFDGVIVCLSLMEL----GLSNVEG-LSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYQDYVCKIAF---DCQLPRWHMKDFFHSFLIVFRVLCGEWIETMWDCMEVAGQPLCILVFMLVMVIGNLVVLNLFLALLLSSFSSDNLSA-PDEDGDLNNIQIAIARINSGISWLFRNIIGFFNRSLRGEKNKTKEANESIKVAVNHTESNGGIYGEKYITPDEDSYITNPNLTVMVPIAPGESDVEFLEEEENSESSDDEEKAEKISLSEGSTVDLRKPGEEADDLSELSEDSM----EPEDCFPQ----FCLRHCQCCNIDISTGLGLAWWRLRKTCYQIVEHSWFETFIIFMILLSSGALAFEDIYIERRKVIKVILEYADKVFSYIFVLEMFLKWIAYGFKKYFTNYWCWLDFLIVDVSLISLVANSLGYSDFAAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGKCVNRTGFIHSITIVNNKSDCLAMNDTQ-FYWTTVKVNFDNVGLGYLSLLQVATFKGWMEIMNAAVDSRGVEEQPSREINLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKRKLGGH-DLFMTEEQKKYYNAMKKLGSKKPQKPIPRPANILQAFFFDLVSKQAFDIMIMMLIIVNMVTMMVETDEQSEQMESILNTINLVFIVIFTTECLIKIFALRCYFFTVAWNIFDFVVIILSIVGIVLADIIEKYFVSPTLFRVIRLARIGRVLRLIRAAKGIRTLLFALMMSMPALFNIGLLLFLVMFIYAIFGMANFAYVKKQDGIDDMFNFETFGNSIICLFQISTSAGWDNLLSPIMSNSPEECDIHFINTGTNTRGNCGNPSMGIAFFVSYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFEMFYEVWEKFDSEATQFIEFSMLSSFADALSEPLRIAKPNKIHLISMDLPMVSGDRIHCLDILFAFTKRVLGE 1705 HSP 2 Score: 207.994 bits (528), Expect = 3.040e-53 Identity = 126/288 (43.75%), Postives = 174/288 (60.42%), Query Frame = 2
Query: 638 FSFFVLTTIIINCIAMTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFD---NSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSN-NVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
F F+ T I + M FT IYT E IK ARG FT+LRD WNWLDFIVI +AY+ +++ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCV+ NSS T + W+ D+ N +N+ C + +EC++ NPN+ YTSFD FG + L FRLMTQDYWE L+ +R+ G +M++F++VIF GSFYL+N+IL++VAM+Y
Sbjct: 34 FCLFLKTEICLRAALMGCILSRHAYRYTFTGIYTFESAIKIFARGFCTMPFTFLRDPWNWLDFIVIVMAYVTEFID--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGLLRQKCVRSLGHCINSSYIPNTTFVCNNRTWSSPTDFLTNEDNFYKTEGAKDGLICGYGSDAGKCPDGFECLKV-GRNPNYDYTSFDTFGWAFLALFRLMTQDYWEELFHQTLRSAGKTYMVFFVLVIFLGSFYLVNLILAVVAMAY 318 HSP 3 Score: 81.6481 bits (200), Expect = 9.197e-15 Identity = 47/136 (34.56%), Postives = 78/136 (57.35%), Query Frame = 2
Query: 3644 FTVIFLVEMVLKWFAIGLTK----YFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVDSNNVRLHESLVQNKS 4036
FT I+ E +K FA G + +D W WLDF ++++A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV S ++ S + N +
Sbjct: 62 FTGIYTFESAIKIFARGFCTMPFTFLRDPWNWLDFIVIVMAYVTEF--IDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGLLRQKCVRSLGHCINSSYIPNTT 195 HSP 4 Score: 62.3882 bits (150), Expect = 6.136e-9 Identity = 75/322 (23.29%), Postives = 143/322 (44.41%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAMTKNS---ESENV--------ETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALG-----KXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTS----FDNFGVSLLCAFRLMTQDYWENLYQLIIRTNG---------DVHM-IYFMMVIFFGSFYLINVILSIV 1474
LR +I+ HS F F++ I+++ A+ E V + +F+ I+ E +K +A G Y Y W WLDF+++ ++ + V ++LG + +LR+LR LR L+ ++ GM+ VV++L+ AI + + L++ + IF+++G+ ++ G KCV N F H+ T N + +C+ ++ F +T+ FDN G+ L ++ T W + + + G +++M IYF++ I FGSF+ +N+ + ++
Sbjct: 1037 LRKTCYQIVEHSWFETFIIFMILLSSGALAFEDIYIERRKVIKVILEYADKVFSYIFVLEMFLKWIAYGFKKYFTNY----WCWLDFLIVDVSLISLVANSLGYSDFAAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK-FGKCV-----------------------------------NRTGFIHSIT-------IVNNKSDCLAMNDT--QFYWTTVKVNFDNVGLGYLSLLQVATFKGWMEIMNAAVDSRGVEEQPSREINLYMYIYFVVFIIFGSFFTLNLFIGVI 1309 HSP 5 Score: 58.151 bits (139), Expect = 1.177e-7 Identity = 66/241 (27.39%), Postives = 114/241 (47.30%), Query Frame = 2
Query: 2114 EGLYNNINEP-EMKKRISALSVFECENKLRKRDIYLQRFNKTFC--NWECCP---------AYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSI-DTYDNSVSMI-YIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEF 2794
EG ++ +P E +S LS E+ + D + Q FC + +CC A+ L+K IV + E FI I+L++ ++ D Y +I I+ + F IF E +K IAYG + YF+ W DFLIV +S++ L A+ + + LRT R LR + ++ M ++ + + +I N+ +V I IF+++G+ +F ++ + N+T F
Sbjct: 981 EGSTVDLRKPGEEADDLSELS----EDSMEPEDCFPQ-----FCLRHCQCCNIDISTGLGLAWWRLRKTCYQIVEHSWFETFIIFMILLSSGALAFEDIYIERRKVIKVILEYADKVFSYIFVLEMFLKWIAYGFKKYFTNYWCWLDFLIVDVSLISLVANSLGYSDFAAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGKCVNRTGF 1212
BLAST of Sodium channel protein vs. Ensembl Medaka
Match: scn12aa (sodium channel protein type 4 subunit alpha [Source:NCBI gene;Acc:101159774]) HSP 1 Score: 870.922 bits (2249), Expect = 0.000e+0 Identity = 533/1101 (48.41%), Postives = 737/1101 (66.94%), Query Frame = 2
Query: 2225 FNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNG-IYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHD-----PQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCS---ESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKID--------KKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
F K + W CCP + LK+ V F+V+DPF++L ITICIVLNT+FM+++ Y + +++ NL F IFT E +KL A P YF + WN+FD +IV LS++EL +S +G LS+LR+FRLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+FG+ Y+ + + F + ++PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G CI F+ M++GNLV+LNLFLALLLSSFSS++L DE D+N +Q A+ R+ I W+ + +N + +R + ++NES IK+ N TE + ++ P + N L P + D+ E ++ D ++ C +RHC CC+ + + AWW +R + ++IV+H +FETFI+ MI+ SS L ED ++ RK +K ILE+ D F+ IF++EM LKW A G KYF + WCWLDF IV V+LIS D A K++RTLRALRPLRA+SR++GM++VVNALI AIPSI NVLLVCL+FWLIFSI+G+ LF GKF KCV+ ++V NKSDC + T+ W ++NFDNV GYLSL Q+ATFKGW +IM AVD V QP N + YIYFV+FIIFG+FFTLNLFIGVIIDNFN QK+KI G +LFMT+ QK+YY+AMKK+ S PQKP+PRP + + F+++++ Q FDI+IM I++N + M +E+ +Q +++ L+ +N VF++IF EC+IKI ALR Y+F + WN+FDF+++I SI V+ + VSPTL RVIR+ RIGRVLRL+++A+GIRTLLF+L++S+PALFNIGLLL L+MFIYAI G++ F+ V GID FNFETFG ++I LFQISTS GW+++L +M+N+ EC I + CG+ + + + VSY++ISFL+++NMYIA+ILENFS AT++ L++DD+++FYE WE++D AT F+E LS F + EPL I +PNKI L +++P+ G I C+D++ TK LGE
Sbjct: 494 FAKRYLIWSCCPWWLKLKEWVKFMVMDPFLDLGITICIVLNTLFMALEHYPMTDEFNTMLSVGNLVFTGIFTAEMVLKLTALDPYYYFQQGWNIFDGVIVCLSLMEL----GLSNVEG-LSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYQDYVCKIAF---DCQLPRWHMKDFFHSFLIVFRVLCGEWIETMWDCMEVAGQPLCILVFMLVMVIGNLVVLNLFLALLLSSFSSDNLSA-PDEDGDLNNIQIAIARINSGISWLFRNIIGFFN-------RSLRGEKNKTK----EANES---------IKVAVNHTESNGGIYGEKYITPDEDSYITNPNLTVMVPIAPGESDVEFLEEEENSESSDDEEKAVIC--LRHCQCCNIDISTGLGLAWWRLRKTCYQIVEHSWFETFIIFMILLSSGALAFEDIYIERRKVIKVILEYADKVFSYIFVLEMFLKWIAYGFKKYFTNYWCWLDFLIVDVSLISLVANSLGYSDFAAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGKCVNRTGFIHSITIVNNKSDCLAMNDTQ-FYWTTVKVNFDNVGLGYLSLLQVATFKGWMEIMNAAVDSRGVEEQPSREINLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKRKISGH-DLFMTEEQKKYYNAMKKLGSKKPQKPIPRPANILQAFFFDLVSKQAFDIMIMMLIIVNMVTMMVETDEQSEQMESILNTINLVFIVIFTTECLIKIFALRCYFFTVAWNIFDFVVIILSIVGIVLADIIEKYFVSPTLFRVIRLARIGRVLRLIRAAKGIRTLLFALMMSMPALFNIGLLLFLVMFIYAIFGMANFAYVKKQDGIDDMFNFETFGNSIICLFQISTSAGWDNLLSPIMSNSPEECDIHFINTGTNTRGNCGNPSMGIAFFVSYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFEMFYEVWEKFDSEATQFIEFSMLSSFADALSEPLRIAKPNKIHLISMDLPMVSGDRIHCLDILFAFTKRVLGE 1561 HSP 2 Score: 283.108 bits (723), Expect = 1.776e-76 Identity = 173/385 (44.94%), Postives = 236/385 (61.30%), Query Frame = 2
Query: 407 LEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFY-NDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSL-FS--------FFVLTTIIINCIAMTKNSE---SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMM-YVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIK-----------STCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
LEAGK LP GD P+ G LED+DPFY +++TFVV+N K IFRFSST A ++FSPF+ +R+ AI+IL HS FS +F++ TI+ NC M + ++ +E FT IYT E IK ARG FT+LRD WNWLDFIVI +AY+ +++ LG +SALR+ RVLRALKT++ IPG+KT+V +L+ ++ KL D ++LTVF LS+FALIGLQ++ G L KCV+ + I Y N TF +NF K C + +EC++ NPN+ YTSFD FG + L FRLMTQDYWE L+ +R+ G +M++F++VIF GSFYL+N+IL++VAM+Y
Sbjct: 52 LEAGKQLPRIFGDIPPE--LVGVPLEDIDPFYYRNQKTFVVLNKGKAIFRFSSTSALYIFSPFHFIRSIAIKILVHSYPFSATITDSIFYFIMFTILTNCFFMAMSDPPTWTKYLEYTFTGIYTFESAIKIFARGFCTMPFTFLRDPWNWLDFIVIVMAYVTEFID--LGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGLLRQKCVRSLGHC----------------INSSYIPN-------TTFVYNFYKTEGAKDGLICGYGSDAGKCPDGFECLKV-GRNPNYDYTSFDTFGWAFLALFRLMTQDYWEELFHQTLRSAGKTYMVFFVLVIFLGSFYLVNLILAVVAMAY 408 HSP 3 Score: 91.2781 bits (225), Expect = 1.021e-17 Identity = 68/227 (29.96%), Postives = 113/227 (49.78%), Query Frame = 2
Query: 3485 WIRCSAFKIVDHKF-FET--------FILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTK----YFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLS 4123
+IR A KI+ H + F FI+ I+ + + + D PT + +++ FT I+ E +K FA G + +D W WLDF ++++A ++ F D G A+RT R LR L+ +S G+K +V ALIQ++ + +V+++ + +F++IG+QLF G KCV S ++ S + N + Y KTE D ++ GY S
Sbjct: 114 FIRSIAIKILVHSYPFSATITDSIFYFIMFTILTNCFFMAMSDP--PTWTK------YLEYTFTGIYTFESAIKIFARGFCTMPFTFLRDPWNWLDFIVIVMAYVTEF--IDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMILTVFCLSVFALIGLQLFMGLLRQKCVRSLGHCINSSYIPNTTFVYNFYKTEGAK--------DGLICGYGS 322 HSP 4 Score: 62.003 bits (149), Expect = 6.811e-9 Identity = 74/322 (22.98%), Postives = 143/322 (44.41%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAMTKNS---ESENV--------ETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALG-----KXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTS----FDNFGVSLLCAFRLMTQDYWENLYQLIIRTNG---------DVHM-IYFMMVIFFGSFYLINVILSIV 1474
LR +I+ HS F F++ I+++ A+ E V + +F+ I+ E +K +A G Y + W WLDF+++ ++ + V ++LG + +LR+LR LR L+ ++ GM+ VV++L+ AI + + L++ + IF+++G+ ++ G KCV N F H+ T N + +C+ ++ F +T+ FDN G+ L ++ T W + + + G +++M IYF++ I FGSF+ +N+ + ++
Sbjct: 893 LRKTCYQIVEHSWFETFIIFMILLSSGALAFEDIYIERRKVIKVILEYADKVFSYIFVLEMFLKWIAYGFK----KYFTNYWCWLDFLIVDVSLISLVANSLGYSDFAAIKSLRTLRALRPLRALSRFEGMRVVVNALIGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGK-FGKCV-----------------------------------NRTGFIHSIT-------IVNNKSDCLAMNDT--QFYWTTVKVNFDNVGLGYLSLLQVATFKGWMEIMNAAVDSRGVEEQPSREINLYMYIYFVVFIIFGSFFTLNLFIGVI 1165
BLAST of Sodium channel protein vs. Ensembl Medaka
Match: ENSORLT00000044829.1 (sodium channel protein type 2 subunit alpha [Source:NCBI gene;Acc:101155239]) HSP 1 Score: 865.529 bits (2235), Expect = 0.000e+0 Identity = 546/1182 (46.19%), Postives = 763/1182 (64.55%), Query Frame = 2
Query: 2021 QLSSNKINKVDSKVNFIERESRISNIVLEDL--EGLYNNINEPEMKKRISALS--------VFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTY---DNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIY-CIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNH-IGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMN------KNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD-SNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
Q SN+I+ +K I R++ +S + + + +G N + P+ K R A S V E E +K +F + F W+CCPA+ +KK V IV+DPF +L ITICIVLNT+FM+++ + D M+ I NKV F IFT E +K+IA P YF E+WN+FD +IV +S+VEL S ++LLR+ RLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+F R+Y N ++ D + PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G+ C+ ++ M++GNLV + F + + ++ +E +D+N LQ A+ R+ R ++K L + Y + K K I + + +N S + + A ++ + ++ + S + D +L+ + + +YE AD E E N P C P F + + KAWW +R + F+IV+H +FE+FI+ MI+ SS L ED + RK +K ILEF D FT IF++EM+LKW A G KYF +AWCWLDF IV V+L+S ++ D GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSIFNVLLVCL+FWLIFSI+G+ LF GKF+KC++ ++ V + NK+ C ++ W N +IN+DNV GYL+L Q+ATFKGW DIM AVD E + QP + N Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKKI S +LFMT+ QK+YY+AMKK+ S PQKP+PRP + I ++II+ Q FDIVIM I LN + M +E+ Q ++ + L +N VF++IF+ EC++K+I LR Y+F WN+FD ++VI SI + K + VSPTL RVIR+ RIGRVLRL+KSA+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TSGGW+++LD ++N+N +C +K+ CG+ + + + VSY++ISFL+++NMYIAVILENF+ AT++ L++DD+++FYE WE +DPGAT F+ LS+F + PL +P+PN+I L +++PI G I C+D++ TK LGE
Sbjct: 489 QAFSNRIHNDSNKE--ILRKAVLSLLYMSSIIKKGHKNMLQTPKNKARQRAQSLVSVITSTVEELEESRKKCPPCWYKFAENFLIWDCCPAWQKIKKFVKLIVMDPFADLTITICIVLNTLFMAMEHHPMQDEFTKMLSIGNKV---FTGIFTAEMVLKIIALDPYYYFQERWNIFDGIIVTISLVELCFS---------INLLRSLRLLRVFKLAKSWPTLNSLLKIIGNSVGAMGNLTLVLAIIVFIFAVVGMQLFEREYRY--NVSKISAD--RKPRWHMMDFFHSFLIVFRVLCGEWIETMWDCMEVAGVTKCLIVYMMVMVVGNLVFTSCFHSFSADNLAAI------EEDSDLNNLQIAIKRICRGFDFIKSFLKNIYKSLVDKSGDKYIVNSKSNDSIMSFINNPSLTVTVPIAAVESDFEVLNTEDFSSASSDDEDEELSSSEGST-INDYEAGEIADSVGGELEES--NDPSPCFPAGENWFCLN-----VDVGFWKAWWKLRQTCFRIVEHSWFESFIIFMILLSSGALAFEDVYSEQRKTIKIILEFADKMFTYIFILEMLLKWLAYGFAKYFTNAWCWLDFLIVDVSLVSLVANALELTDLGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIFNVLLVCLIFWLIFSIMGVNLFAGKFYKCINKTDGVECDPENIPNKTFC---TNETDLIWKNLKINYDNVGMGYLALLQVATFKGWMDIMYAAVDSGEPDEQPVFERNIKMYLYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKI--STDLFMTEDQKKYYNAMKKLGSKKPQKPIPRPSNKIQGFIFDIISKQAFDIVIMVLIWLNMVTMMVETADQSEKQTYILRVINYVFIVIFSGECLLKMIGLRHYFFMNGWNIFDLVVVILSIAGVFLAKIIEDYFVSPTLFRVIRLARIGRVLRLIKSAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSSFAYVKKDKGIDDLFNFETFGNSMICLFQITTSGGWDTLLDPILNSNIDDCDRNKEHPGSNTKGDCGNPPVGIAFFVSYIIISFLIVVNMYIAVILENFNVATEESTDPLSEDDFEMFYEVWERFDPGATQFIHYYSLSDFADALNPPLQVPKPNRIDLIFMDLPIVSGDRIHCLDILFAFTKRVLGE 1633 HSP 2 Score: 276.944 bits (707), Expect = 1.692e-74 Identity = 160/369 (43.36%), Postives = 236/369 (63.96%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSES---ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNP---ENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
++ L AGK LP GD LE+LDP Y +++TF+V+N K IFRF++ A + PFNP+R +I+IL HS + L + I+C + +S S + VE FTAIYT E ++K LARG + +FT+LRD WNWLDF VI LAY+ + G+LSALR+ RVLRA K ++ IPG+KT+V +L ++ KL D ++LTVF LS+FAL+GLQ++ G L +KCV+ ++ + + + + ++++++ N ++P+ N+ C E Y C+ + P+FGYTSFD+FG + L FRLMTQD+WENLYQ +R +G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 52 NRDLMAGKPLPILYGDI--PNGLVSTPLEELDPAYYNQKTFIVLNKRKMIFRFNAAPALYFLGPFNPIRRLSIKILVHS----YPLHKVDIDCELICADSNSTWTKPVEYTFTAIYTFESLVKILARGFCLGEFTFLRDPWNWLDFSVIVLAYLNEF-ADFGRLSALRTFRVLRAFKAISVIPGLKTIVGALFQSVKKLTDVMILTVFCLSVFALVGLQLFMGHLKNKCVRQPEHQYMANDQFLYLMGVFHSVELNFQCNHYFLSGKRDPL-----LCGNSTYAGKCPEGYTCV-SFGSTPDFGYTSFDSFGWAFLALFRLMTQDFWENLYQQTLRASGKPYMIFFVLVIFLGSFYLINLILAVVAMAY 407 HSP 3 Score: 79.7221 bits (195), Expect = 3.367e-14 Identity = 41/120 (34.17%), Postives = 74/120 (61.67%), Query Frame = 2
Query: 3644 FTVIF----LVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV 3988
FT I+ LV+++ + F +G + +D W WLDF+++++A ++ F D G A+RT R LR +A+S G+K +V AL Q++ + +V+++ + +F+++G+QLF G KCV
Sbjct: 157 FTAIYTFESLVKILARGFCLGEFTFLRDPWNWLDFSVIVLAYLNEFA--DFGRLSALRTFRVLRAFKAISVIPGLKTIVGALFQSVKKLTDVMILTVFCLSVFALVGLQLFMGHLKNKCV 274 HSP 4 Score: 76.6406 bits (187), Expect = 2.584e-13 Identity = 85/318 (26.73%), Postives = 147/318 (46.23%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAMT-KNSESENVETI----------FTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSAL-----GKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVHMI---------YFMMVIFFGSFYLINVILSIV 1474
LR RI+ HS F F++ I+++ A+ ++ SE +TI FT I+ E ++K LA G Y +AW WLDF+++ ++ + V +AL G + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G +KC+ N + G E +PEN N TFC N TD N ++DN G+ L ++ T W + +Y + D + YF++ I FGSF+ +N+ + ++
Sbjct: 967 LRQTCFRIVEHSWFESFIIFMILLSSGALAFEDVYSEQRKTIKIILEFADKMFTYIFILEMLLKWLAYGFA----KYFTNAWCWLDFLIVDVSLVSLVANALELTDLGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIFNVLLVCLIFWLIFSIMGVNLFAGKF-YKCI----NKTDGVEC-----------------DPENIPNK-TFCTN------------------ETDLIWKNLK-INYDNVGMGYLALLQVATFKGWMDIMYAAVDSGEPDEQPVFERNIKMYLYFVVFIIFGSFFTLNLFIGVI 1238
BLAST of Sodium channel protein vs. Ensembl Medaka
Match: ENSORLT00000035153.1 (sodium channel protein type 2 subunit alpha [Source:NCBI gene;Acc:101155239]) HSP 1 Score: 858.981 bits (2218), Expect = 0.000e+0 Identity = 587/1320 (44.47%), Postives = 812/1320 (61.52%), Query Frame = 2
Query: 1724 LPRTKSL----QEISKLNVDQK---SDIAESKHKES--NDNMLIDSCPSLHRTNRFKVYQQF------NVFR----KLIEDNRKESSKYS--LDDKRFSDLASCQVNTSKA------QHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTY---DNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIY-CIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQI----------KYPFPWDSNES------NP---INIQAAIIKMN------KNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLP----DSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISY----FKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVD-SNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKK--------CGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGE 5464
+P++ SL +EIS L D SD+ S +S N + S +T+ F Q N F + E+NR S S L D+ S L+ C +H S+ N +S +R S + I+ D+E +N +K +F + F W+CCPA+ +KK V IV+DPF +L ITICIVLNT+FM+++ + D M+ I NKV F IFT E +K+IA P YF E+WN+FD +IV +S+VEL S ++LLR+ RLLR+FKLAKSW T+N L IIG ++GA+GNL +VLAIIVFIFAVVGMQ+F R+Y N ++ D + PRW+ DF HSF+IVFRVLCGE++E+MWDCM V G+ C+ ++ M++GNLV+LNLFLALLLSSFS+++L E++S D+N LQ A+ R+ R ++K L + N K K + + KY SN+S NP + + A ++ + ++ + S + D +L+ + + +YE AD E E N P C P +I+C C + KAWW +R + F+IV+H +FE+FI+ MI+ SS L ED + RK +K ILEF D FT IF++EM+LKW A G KYF +AWCWLDF IV V+L+S ++ D GA K++RTLRALRPLRA+SR++GM++VVNAL+ AIPSIFNVLLVCL+FWLIFSI+G+ LF GKF+KC++ ++ V + NK+ C ++ W N +IN+DNV GYL+L Q+ATFKGW DIM AVD E + QP + N Y+YFV+FIIFG+FFTLNLFIGVIIDNFN QKKKI S +LFMT+ QK+YY+AMKK+ S PQKP+PRP + I ++II+ Q FDIVIM I LN + M +E+ Q ++ + L +N VF++IF+ EC++K+I LR Y+F WN+FD ++VI SI + K + VSPTL RVIR+ RIGRVLRL+KSA+GIRTLLF+L++SLPALFNIGLLL L+MFIYAI G+S F+ V GID FNFETFG +MI LFQI+TSGGW+++LD ++N+N +C +K+ CG+ + + + VSY++ISFL+++NMYIAVILENF+ AT++ L++DD+++FYE WE +DPGAT F+ LS+F + PL +P+PN+I L +++PI G I C+D++ TK LGE
Sbjct: 506 IPKSNSLNSLKKEISHLTTDGNLLNSDLKSSTTHQSFLNFRGALISSRRYSKTSLFSFQSQIQDLSSENEFADDEYSIFEENRSGSGSMSHHLHDRHSSSLSECLFLPPLLLQPDGLKHSSIGCNGCMSQK------------DRSSPPTKILSLDVE-----------------------KNSRKKCPPCWYKFAENFLIWDCCPAWQKIKKFVKLIVMDPFADLTITICIVLNTLFMAMEHHPMQDEFTKMLSIGNKV---FTGIFTAEMVLKIIALDPYYYFQERWNIFDGIIVTISLVELCFS---------INLLRSLRLLRVFKLAKSWPTLNSLLKIIGNSVGAMGNLTLVLAIIVFIFAVVGMQLFEREYRY--NVSKISAD--RKPRWHMMDFFHSFLIVFRVLCGEWIETMWDCMEVAGVTKCLIVYMMVMVVGNLVMLNLFLALLLSSFSADNLAAIEEDS-DLNNLQIAIKRICRGFDFIKSFLKNICNSNCHKEKKEKTKDNNVNLTGVDKSGDKYIVNSKSNDSIMSFINNPSLTVTVPIAAVESDFEVLNTEDFSSASSDDEDEELSSSEGST-INDYEAGEIADSVGGELEES--NDPSPCFPAVCLQNIKC-----CQVNVDVGFWKAWWKLRQTCFRIVEHSWFESFIIFMILLSSGALAFEDVYSEQRKTIKIILEFADKMFTYIFILEMLLKWLAYGFAKYFTNAWCWLDFLIVDVSLVSLVANALELTDLGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIFNVLLVCLIFWLIFSIMGVNLFAGKFYKCINKTDGVECDPENIPNKTFC---TNETDLIWKNLKINYDNVGMGYLALLQVATFKGWMDIMYAAVDSGEPDEQPVFERNIKMYLYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKI--STDLFMTEDQKKYYNAMKKLGSKKPQKPIPRPSNKIQGFIFDIISKQAFDIVIMVLIWLNMVTMMVETADQSEKQTYILRVINYVFIVIFSGECLLKMIGLRHYFFMNGWNIFDLVVVILSIAGVFLAKIIEDYFVSPTLFRVIRLARIGRVLRLIKSAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSSFAYVKKDKGIDDLFNFETFGNSMICLFQITTSGGWDTLLDPILNSNIDDCDRNKEHPGSNTKGDCGNPPVGIAFFVSYIIISFLIVVNMYIAVILENFNVATEESTDPLSEDDFEMFYEVWERFDPGATQFIHYYSLSDFADALNPPLQVPKPNRIDLIFMDLPIVSGDRIHCLDILFAFTKRVLGE 1760 HSP 2 Score: 288.115 bits (736), Expect = 6.804e-78 Identity = 168/383 (43.86%), Postives = 241/383 (62.92%), Query Frame = 2
Query: 398 DKRLEAGKVLPHYLGDYFPKKKFAGKMLEDLDPFYNDKRTFVVINSSKTIFRFSSTKAFFLFSPFNPLRAAAIRILTHS-------------LFSFFVLTTIIINCIAMTKNSESEN-------VETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSY 1486
++ L AGK LP GD LE+LDP Y +++TF+V+N K IFRF++ A + PFNP+R +I+IL HS LF+ ++ TI+ NC MT++S +++ VE FTAIYT E ++K LARG + +FT+LRD WNWLDF VI LAY+ + G+LSALR+ RVLRA K ++ IPG+KT+V +L ++ KL D ++LTVF LS+FAL+GLQ++ G L +KCV+ ++ + E IL+ N ++Y ++P+ N+ C E Y C+ + P+FGYTSFD+FG + L FRLMTQD+WENLYQ +R +G +MI+F++VIF GSFYLIN+IL++VAM+Y
Sbjct: 52 NRDLMAGKPLPILYGDI--PNGLVSTPLEELDPAYYNQKTFIVLNKRKMIFRFNAAPALYFLGPFNPIRRLSIKILVHSYPLHKVDIDSPCRLFNVLIMCTILTNCALMTRSSSADSNSTWTKPVEYTFTAIYTFESLVKILARGFCLGEFTFLRDPWNWLDFSVIVLAYLNEF-ADFGRLSALRTFRVLRAFKAISVIPGLKTIVGALFQSVKKLTDVMILTVFCLSVFALVGLQLFMGHLKNKCVRQPEHVTPEENLTEYWRILIENANSNHYF-LSGKRDPL-----LCGNSTYAGKCPEGYTCV-SFGSTPDFGYTSFDSFGWAFLALFRLMTQDFWENLYQQTLRASGKPYMIFFVLVIFLGSFYLINLILAVVAMAY 424 HSP 3 Score: 80.1073 bits (196), Expect = 2.283e-14 Identity = 44/134 (32.84%), Postives = 80/134 (59.70%), Query Frame = 2
Query: 3644 FTVIF----LVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV-DSNNVRLHESLVQ 4027
FT I+ LV+++ + F +G + +D W WLDF+++++A ++ F D G A+RT R LR +A+S G+K +V AL Q++ + +V+++ + +F+++G+QLF G KCV +V E+L +
Sbjct: 178 FTAIYTFESLVKILARGFCLGEFTFLRDPWNWLDFSVIVLAYLNEFA--DFGRLSALRTFRVLRAFKAISVIPGLKTIVGALFQSVKKLTDVMILTVFCLSVFALVGLQLFMGHLKNKCVRQPEHVTPEENLTE 309 HSP 4 Score: 76.2554 bits (186), Expect = 3.407e-13 Identity = 82/318 (25.79%), Postives = 148/318 (46.54%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAMT-KNSESENVETI----------FTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSAL-----GKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWEN-LYQLIIRTNGDVHMI---------YFMMVIFFGSFYLINVILSIV 1474
LR RI+ HS F F++ I+++ A+ ++ SE +TI FT I+ E ++K LA G Y +AW WLDF+++ ++ + V +AL G + +LR+LR LR L+ ++ GM+ VV++LL AI + + L++ + IF+++G+ ++ G +KC+ N + G E +PEN N K+ C E + I + + ++DN G+ L ++ T W + +Y + D + YF++ I FGSF+ +N+ + ++
Sbjct: 1094 LRQTCFRIVEHSWFESFIIFMILLSSGALAFEDVYSEQRKTIKIILEFADKMFTYIFILEMLLKWLAYGFA----KYFTNAWCWLDFLIVDVSLVSLVANALELTDLGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIFNVLLVCLIFWLIFSIMGVNLFAGKF-YKCI----NKTDGVEC-----------------DPENIPN--------------KTFCTNETDLIWKNLK------INYDNVGMGYLALLQVATFKGWMDIMYAAVDSGEPDEQPVFERNIKMYLYFVVFIIFGSFFTLNLFIGVI 1365
BLAST of Sodium channel protein vs. Planmine SMEST
Match: SMESG000077545.1 (SMESG000077545.1) HSP 1 Score: 3019.18 bits (7826), Expect = 0.000e+0 Identity = 1610/1650 (97.58%), Postives = 1611/1650 (97.64%), Query Frame = 2
Query: 683 MTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDDKRFSDLASCQVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKKENI 5632
MTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKLSALRSLRVLRALKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYDDVRMADMAEDEEEEAEKKVEEEKREQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDDKRFSDLAS VNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCIVLAIIVFIFAVVGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMILGNLVILNLFLALLLSSFSSESLQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTFYYIYFVLFIIFGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTLIRVIRVFRIGRVLRLVKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNT +ILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLKPKSKNKITSKKFVISNTFLYQRELWAARKITGFLREMVARKKENI
Sbjct: 1 MTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKLSALRSLRVLRALKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYDDVRMADMAEDEEEEAEKKVEEEKREQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDDKRFSDLASSPVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCIVLAIIVFIFAVVGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMILGNLVILNLFLALLLSSFSSESLQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTFYYIYFVLFIIFGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTLIRVIRVFRIGRVLRLVKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTSSVS--------------------------------CIILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLKPKSKNKITSKKFVISNTFLYQRELWAARKITGFLREMVARKKENI 1618
BLAST of Sodium channel protein vs. Planmine SMEST
Match: SMESG000077545.1 (SMESG000077545.1) HSP 1 Score: 3007.62 bits (7796), Expect = 0.000e+0 Identity = 1607/1650 (97.39%), Postives = 1609/1650 (97.52%), Query Frame = 2
Query: 683 MTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDDKRFSDLASCQVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKKENI 5632
MTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKLSALRSLRVLRALKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYDDVRMADMAEDEEEEAEKKVEEEKREQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDDKRFSDLAS VNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCIVLAIIVFIFAVVGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMILGNLVILNLFLALLLSSFSSESLQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTFYYIYFVLFIIFGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTLIRVIRVFRIGRVLRLVKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILD M LY S + +ILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLKPKSKNKITSKKFVISNTFLYQRELWAARKITGFLREMVARKKENI
Sbjct: 1 MTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKLSALRSLRVLRALKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYDDVRMADMAEDEEEEAEKKVEEEKREQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDDKRFSDLASSPVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCIVLAIIVFIFAVVGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMILGNLVILNLFLALLLSSFSSESLQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTFYYIYFVLFIIFGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTLIRVIRVFRIGRVLRLVKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDEKMWK-------------------LYFSSSVS-----------CIILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLKPKSKNKITSKKFVISNTFLYQRELWAARKITGFLREMVARKKENI 1620
BLAST of Sodium channel protein vs. Planmine SMEST
Match: SMESG000077545.1 (SMESG000077545.1) HSP 1 Score: 2266.5 bits (5872), Expect = 0.000e+0 Identity = 1199/1239 (96.77%), Postives = 1200/1239 (96.85%), Query Frame = 2
Query: 1916 KESSKYSLDDKRFSDLASCQVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKKENI 5632
KESSKYSLDDKRFSDLAS VNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCIVLAIIVFIFAVVGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMILGNLVILNLFLALLLSSFSSESLQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTFYYIYFVLFIIFGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTLIRVIRVFRIGRVLRLVKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNT +ILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLKPKSKNKITSKKFVISNTFLYQRELWAARKITGFLREMVARKKENI
Sbjct: 5 KESSKYSLDDKRFSDLASSPVNTSKAQHDSLILNHQLSSNKINKVDSKVNFIERESRISNIVLEDLEGLYNNINEPEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCIVLAIIVFIFAVVGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMILGNLVILNLFLALLLSSFSSESLQRREDESADVNKLQEALTRVYRAIVWVKVKLYSTYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKNETEPSRNVHDPQLNQVAFNLWLENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNVRLHESLVQNKSDCYELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTFYYIYFVLFIIFGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTLIRVIRVFRIGRVLRLVKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTSSVS--------------------------------CIILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLGEFNSGNPMMDLKPKSKNKITSKKFVISNTFLYQRELWAARKITGFLREMVARKKENI 1211 HSP 2 Score: 78.5666 bits (192), Expect = 4.306e-14 Identity = 82/314 (26.11%), Postives = 140/314 (44.59%), Query Frame = 2
Query: 599 LRAAAIRILTHSLFSFFVLTTIIINCIAMTKNSE-----------SENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNS-ALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRT----------NGDVHMIYFMMVIFFGSFYLINVILSIV 1474
+R +A +I+ H F F+L I+ + + +T + E ++ FT I+ E ++K A GL Y +DAW WLDF ++ +A + Y G A+R+LR LR L+ V+ GMK VV++L+ AI + + L++ + IF++IG+Q++ G KCV DS N + + N KS C YE +T+N + +FDN L F++ T W ++ + N + IYF++ I FG+F+ +N+ + ++
Sbjct: 529 IRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV---------------DS------------------NNVRLHESLVQN---KSDC---YELQKTENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTFYYIYFVLFIIFGAFFTLNLFIGVI 798
BLAST of Sodium channel protein vs. Planmine SMEST
Match: SMESG000017743.1 (SMESG000017743.1) HSP 1 Score: 1596.25 bits (4132), Expect = 0.000e+0 Identity = 971/1715 (56.62%), Postives = 1212/1715 (70.67%), Query Frame = 2
Query: 683 MTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSIL---LWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDA-TSDDLAFC---------AIGDHPLYYPDNIIKSCID------------IRIQNLPKSHVSLYLPRTKSLQEI-------SKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRKESSKYSLDDKRFSDLASCQVNTSKAQH--DSLILNHQLSSNKINKVDSKVNF---IERESRISNIVL---EDLEGLYNNINE----PEMKKRISALSVFECENKLRKRDIYLQRFNKTFCNWECCPAYDYLKKIVSFIVLDPFMELFITICIVLNTVFMSIDTYDNSVSMIYIMNKVNLFFMVIFTFEAGMKLIAYGPRLYFSEKWNVFDFLIVVLSIVELTASKDISASKGNLSLLRTFRLLRIFKLAKSWQTMNLLFTIIGRTLGAIGNLCXXXXXXXXXXXXXGMQIFGRQYERFKNQTEFPKDNGKIPRWNFCDFTHSFMIVFRVLCGEYMESMWDCMRVNGIYCIPFFLATMXXXXXXXXXXXXXXXXXXXXXXXXQRREDESADVNKLQEALTRVYRAIVWVKVKLY----STYNHIGKKLMKIIRRHRQIKYPFPWDSNESNPINIQAAIIKMNKN--ETEPSRNVHDPQLNQVAFNLWL-----ENYEPKFDADPDINEAEVIFWNQPYDCLPDSIRCFLVRHCNCCSESCITKAWWWIRCSAFKIVDHKFFETFILIMIVASSLTLTLEDKFLPTRKRLKDILEFMDLGFTVIFLVEMVLKWFAIGLTKYFKDAWCWLDFTIVLVALISYFKVF------DAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFFKCVDSNNV-RLHESLVQNKSDCYELQKT-ENVSWVNSQINFDNVLNGYLSLFQIATFKGWTDIMADAVDVTEVNFQPKYNNNTXXXXXXXXXXXXGAFFTLNLFIGVIIDNFNVQKKKIGGSLELFMTDSQKRYYHAMKKMSSISPQKPVPRPKSTIAQIFYNIINNQKFDIVIMCFIMLNTIVMCLESHKQGKELSDALDHMNKVFVLIFALECVIKIIALRWYYFKIPWNVFDFIIVIFSIGFWVIEKFVIFNAVSPTXXXXXXXXXXXXXXXXXKSARGIRTLLFSLVVSLPALFNIGLLLMLIMFIYAIIGVSMFSEVAYSYGIDATFNFETFGKAMIILFQISTSGGWNSILDGLMNNNTYECKIDKKKCGSYILAVLYLVSYLVISFLVIINMYIAVILENFSQATKDVQLGLTQDDYDLFYECWEEYDPGATGFMELKDLSEFVAKIGEPLGIPQPNKIRLAMLEIPICKGHTICCVDLMDKLTKNFLG---EFNSGNPMMDLXXXXXXXXXXXXFVISNTFLYQRELWAARKITGFLREMVARKKEN 5629
M KNS S+ VE +FT IYT EF IKA++RGLI+ +FTYLRDAWNWLDFIVIGLAY+M+ LG LSALRSLRVLRALKTVTT+PGMKTVV +LL A+ +LRDA+VLTVF+LSIF LIGLQ+YRGTLL KCVK F+++ + E E +++ W +K +++NN NW +P+ C N+T N ++C E Y CI+T NPN+GYT+FDNF VSLLCAFRLMTQDYWENLYQL IRTNGD+H++YFMMVIFFGSFYLINVIL+IVAMSYDDVR+A AE+ +EAE+K +EE RE+I++ +D EL +S+DL + ++IKS ++ + I++ P+ + Y ++L S K + A+ E+ +L S S+ R + + F ++++ SSK LD S + + QLS N +V ++F +E ES S+I + +D + + + IN PE++ +SALS+++ E K+RK+++ Q KTFC+W CCP Y + +V+ I+LDPF+ELFIT+CIV+NTV MSIDT DNS M + + N FF F EA +KLIA GP YF E WNVFDF+IV+LS+VE I +SLLR FRLLR+FKLAKSWQTMNLLF IIG T+GAIGNLCIVL II+FIFAVVGMQ+FG +Y FKN+T FP G PRWNF DFTHSFMIVFRVLCGE++ESMWDCM VNG C+PFFL TMILGNLV+LNLFLALLLSSF SESLQ+RED+S + NKLQEA+ R+YRAIVWVKV++ + G+ ++K +R + IK W+++ IK K+ E S +V P+ LW E PKFD DP+INE E+I+ P DCLP+ I L + + E+ + WW RC A K+V+H+FFETFI++MI+ SSLTLTLEDK LP+R LK LE +D FT+IF EM+LKWFA GL KYFKDAWCWLDF IV +AL+S + GAFKA+RTLRALRPLRAVSRW+GM+IVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKF+KCV+++ R+ ++ NK+ C ++ NVSW NSQ+NFDNVLNGYL LFQ+ATFKGW +IMADAVD T + QP YN++T YY YFV FIIFGAFFTLNLFIGVIIDNFNVQKK++GGSLE+FMT+ QK+YY+AMKKMSS +PQKP+PRPK IA I ++I NQKFDI IM IMLNTIVMC+E Q ++L ALD +NKVF++IF E ++K+IA RWYYFK+PWN+FDF+IVI SI FW IE + VSPT IRV+RVFRIGR+LRLVKSA+GIRTLLFSL+VSLPAL NI LLL++IMFIYAI+G+S FS VAYS+ ID FNFETFGKAMIILFQISTSGGWN +L+GLMN + C DK CGSYI+AV+YL+SYL+ISFLVIINMYIAVILENFSQATKDVQ GLTQDDYDLFYECWEEYD A GF+ LK L +FV KI +PLGIP+PN ++LAMLEIPIC+ T+ CVDL++ LT+ FLG E ++G ++ +K ++SNT QRE +AA+ I +++ R K+N
Sbjct: 1 MAKNSNSDTVEYVFTTIYTLEFTIKAISRGLIINEFTYLRDAWNWLDFIVIGLAYLMFFFPVLGNLSALRSLRVLRALKTVTTVPGMKTVVGALLEAVKQLRDAMVLTVFLLSIFGLIGLQLYRGTLLRKCVKSFNHTLLYFEPNEFENLTDAEKWTVKYEFFNNETNWLDPLRQCQNYTYNE-WNTSCPEGYVCIQTKTGNPNYGYTNFDNFIVSLLCAFRLMTQDYWENLYQLTIRTNGDIHILYFMMVIFFGSFYLINVILAIVAMSYDDVRLAIQAEEAGDEAERKEQEEMREKIIDRIDEELGVESSEDLNYQNPVRPNSKDGWSIQSYVRKQSLIKSGLNDEFNSKSYQVSNVDIESEPRFSICDYDSTDRNLDSTRLVCPLESYFKGKIKFNYADKSESEATTPILPRSPRSIAVGKR--IVGSLSSFSPMLDE---RSSKSYLDPNLLHTSNSRMFGMKRITEIDGQGEIKRQLSQNLRRRVSKNLSFRHRMENESINSDIFIKINQDDDNMISEINHYIKNPELQSALSALSIYDAETKVRKKELIKQWLLKTFCSWTCCPCYFKFQSLVALIILDPFVELFITLCIVMNTVLMSIDTPDNSPEMNHFLKCANYFFTTTFGLEAFLKLIALGPEQYFQEHWNVFDFIIVILSLVE------IPLDSSGMSLLRAFRLLRVFKLAKSWQTMNLLFMIIGTTVGAIGNLCIVLGIIIFIFAVVGMQLFGDKYSNFKNETLFPSFKGAEPRWNFNDFTHSFMIVFRVLCGEWIESMWDCMLVNGYQCVPFFLMTMILGNLVLLNLFLALLLSSFGSESLQKREDDSNETNKLQEAIDRIYRAIVWVKVQIRFAIKLSKEIFGRCIVK--KRKKLIKSHPMWNAHN----------IKCEKSYYELSVSNDVKIPEEQLSNPELWFKYSLKETEVPKFDEDPEINEVEIIYSTSPEDCLPNFISKLLRKCYDVVFETPPGRLWWNSRCKASKLVEHQFFETFIILMIIISSLTLTLEDKNLPSRPWLKLALEILDKVFTIIFTAEMLLKWFAFGLNKYFKDAWCWLDFIIVTIALVSLVLSYIGTGAGGMGAFKAMRTLRALRPLRAVSRWEGMRIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFYKCVNNDTFERISAEIIPNKNACLIMKAMYGNVSWENSQLNFDNVLNGYLCLFQVATFKGWIEIMADAVDSTGLGNQPSYNHSTIYYAYFVSFIIFGAFFTLNLFIGVIIDNFNVQKKRVGGSLEMFMTEDQKKYYNAMKKMSSTAPQKPIPRPKLKIANIIFSITINQKFDIFIMSVIMLNTIVMCIEHWDQPEKLKTALDQINKVFIVIFTGEFLLKLIAQRWYYFKVPWNMFDFVIVILSIVFWAIESMLNNIPVSPTFIRVVRVFRIGRILRLVKSAKGIRTLLFSLIVSLPALLNIALLLIMIMFIYAIVGMSFFSNVAYSHSIDEVFNFETFGKAMIILFQISTSGGWNGVLNGLMNEDPKVCNKDKGTCGSYIIAVVYLISYLIISFLVIINMYIAVILENFSQATKDVQEGLTQDDYDLFYECWEEYDTKARGFISLKYLQDFVEKIDKPLGIPKPNSVQLAMLEIPICQNDTVYCVDLLEILTRRFLGQKPEADTGENNLNKLKPDLKIGKVEKIIVSNTLKRQRENFAAKVIVKYMKAYTERLKQN 1691 HSP 2 Score: 77.411 bits (189), Expect = 1.196e-13 Identity = 87/319 (27.27%), Postives = 145/319 (45.45%), Query Frame = 2
Query: 602 RAAAIRILTHSLFSFFVLTTIIINCIAMT---KNSES--------ENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNS-------ALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNG----------DVHMIYFMMVIFFGSFYLINVILSIV 1474
R A +++ H F F++ III+ + +T KN S E ++ +FT I+TAE ++K A GL Y +DAW WLDFI++ +A + V S +G A+R+LR LR L+ V+ GM+ VV++L+ AI + + L++ + IF++IG+Q++ G +KCV I AE N + L I Y N +W+N +FDN LC F++ T W + + + G ++ YF+ I FG+F+ +N+ + ++
Sbjct: 968 RCKASKLVEHQFFETFIILMIIISSLTLTLEDKNLPSRPWLKLALEILDKVFTIIFTAEMLLKWFAFGL----NKYFKDAWCWLDFIIVTIALVSLVLSYIGTGAGGMGAFKAMRTLRALRPLRAVSRWEGMRIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGK-FYKCVNNDTFERISAEIIPNKNACL--IMKAMYGNV-SWEN----------------------------------SQLNFDNVLNGYLCLFQVATFKGWIEIMADAVDSTGLGNQPSYNHSTIYYAYFVSFIIFGAFFTLNLFIGVI 1244
BLAST of Sodium channel protein vs. Planmine SMEST
Match: SMESG000077545.1 (SMESG000077545.1) HSP 1 Score: 763.837 bits (1971), Expect = 0.000e+0 Identity = 412/412 (100.00%), Postives = 412/412 (100.00%), Query Frame = 2
Query: 683 MTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKXXXXXXXXXXXXXKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYXXXXXXXXXXXXXXXXXXXXXXXXXXQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRK 1918
MTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKLSALRSLRVLRALKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYDDVRMADMAEDEEEEAEKKVEEEKREQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRK
Sbjct: 1 MTKNSESENVETIFTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNSALGKLSALRSLRVLRALKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCVKVFDNSSIGAETYENDSILLWNIKMDYYNNPENWQNPITFCHNFTSNNVIKSTCNEEYECIRTDNENPNFGYTSFDNFGVSLLCAFRLMTQDYWENLYQLIIRTNGDVHMIYFMMVIFFGSFYLINVILSIVAMSYDDVRMADMAEDEEEEAEKKVEEEKREQILELLDHELDATSDDLAFCAIGDHPLYYPDNIIKSCIDIRIQNLPKSHVSLYLPRTKSLQEISKLNVDQKSDIAESKHKESNDNMLIDSCPSLHRTNRFKVYQQFNVFRKLIEDNRK 412 HSP 2 Score: 83.5741 bits (205), Expect = 3.397e-16 Identity = 45/120 (37.50%), Postives = 70/120 (58.33%), Query Frame = 2
Query: 3644 FTVIFLVEMVLKWFAIGLT----KYFKDAWCWLDFTIVLVALISYFKVFDAGAFKAIRTLRALRPLRAVSRWDGMKIVVNALIQAIPSIFNVLLVCLVFWLIFSIIGMQLFGGKFF-KCV 3988
FT I+ E ++K A GL Y +DAW WLDF ++ +A + Y G A+R+LR LR L+ V+ GMK VV++L+ AI + + L++ + IF++IG+Q++ G KCV
Sbjct: 14 FTAIYTAEFIIKALARGLIVYQFTYLRDAWNWLDFIVIGLAYMMYVNS-ALGKLSALRSLRVLRALKTVTTIPGMKTVVSSLLMAIIKLRDALVLTVFMLSIFALIGLQMYRGTLLHKCV 132 The following BLAST results are available for this feature:
BLAST of Sodium channel protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Sodium channel protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Sodium channel protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Sodium channel protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Sodium channel protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Sodium channel protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Sodium channel protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Sodium channel protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Sodium channel protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Sodium channel protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Sodium channel protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of Sodium channel protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Sodium channel protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Sodium channel protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30003510 ID=SMED30003510|Name=Sodium channel protein|organism=Schmidtea mediterranea sexual|type=transcript|length=5702bpback to top protein sequence of SMED30003510-orf-1 >SMED30003510-orf-1 ID=SMED30003510-orf-1|Name=SMED30003510-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1810bp MKEIYNKYRILTEESLNQANILYKESIKNEKEEKKTLKKETKQRKIDQTKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|
