SMED30003476

Overview
NameSMED30003476
Smed IDSMED30003476
Length (bp)321
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003476

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30003476h1SMcG0008963 Contig702uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003476h1SMcG0008963 Contig702newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
cephalic gangliaSMED30003476h1SMcG0008963 dd_Smed_v4_9652_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003476h1SMcG0008963 dd_Smed_v4_9652_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30003476h1SMcG0008963 dd_Smed_v4_7746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003476h1SMcG0008963 dd_Smed_v4_7746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003476h1SMcG0008963 dd_Smed_v4_7746_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003476h1SMcG0008963 Contig35475newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003476h1SMcG0008963 Contig35475uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
tail regionSMED30003476h1SMcG0008963 dd_Smed_v6_7746_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
tail regionSMED30003476h1SMcG0008963 dd_Smed_v6_11611_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003476 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000049:120966..121572 +1524
v31.000049:120626..121279 +619
Homology
BLAST of SMED30003476 vs. Ensembl Human
Match: ANKRD28 (ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:HGNC:29024])

HSP 1 Score: 69.3218 bits (168), Expect = 1.353e-14
Identity = 35/101 (34.65%), Postives = 54/101 (53.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH E V +L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + +VK L+D   D+
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDM 232          

HSP 2 Score: 68.1662 bits (165), Expect = 3.375e-14
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH E VKLL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEV 199          

HSP 3 Score: 58.5362 bits (140), Expect = 1.020e-10
Identity = 35/102 (34.31%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  VK L+    D+   +     P+H AC  G   +V  LID
Sbjct:  159 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELID 260          

HSP 4 Score: 46.2098 bits (108), Expect = 1.739e-6
Identity = 28/106 (26.42%), Postives = 52/106 (49.06%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEA----LDQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V+ L++      + D      IH + + GHI  +  L+     ++A     D + +  +H+AC  GH   V+LL++
Sbjct:  672 LLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSAASMDANPATADNHGYTALHWACYNGHETCVELLLE 777          
BLAST of SMED30003476 vs. Ensembl Human
Match: ANKRD28 (ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:HGNC:29024])

HSP 1 Score: 69.3218 bits (168), Expect = 1.353e-14
Identity = 35/101 (34.65%), Postives = 54/101 (53.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH E V +L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + +VK L+D   D+
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDM 232          

HSP 2 Score: 68.1662 bits (165), Expect = 3.375e-14
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH E VKLL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEV 199          

HSP 3 Score: 58.5362 bits (140), Expect = 1.020e-10
Identity = 35/102 (34.31%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  VK L+    D+   +     P+H AC  G   +V  LID
Sbjct:  159 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELID 260          

HSP 4 Score: 46.2098 bits (108), Expect = 1.739e-6
Identity = 28/106 (26.42%), Postives = 52/106 (49.06%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEA----LDQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V+ L++      + D      IH + + GHI  +  L+     ++A     D + +  +H+AC  GH   V+LL++
Sbjct:  672 LLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSAASMDANPATADNHGYTALHWACYNGHETCVELLLE 777          
BLAST of SMED30003476 vs. Ensembl Human
Match: ANKRD28 (ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:HGNC:29024])

HSP 1 Score: 68.5514 bits (166), Expect = 2.960e-14
Identity = 35/101 (34.65%), Postives = 54/101 (53.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH E V +L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + +VK L+D   D+
Sbjct:   76 NVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDM 176          

HSP 2 Score: 67.3958 bits (163), Expect = 6.766e-14
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH E VKLL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:   37 LLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEV 143          

HSP 3 Score: 57.7658 bits (138), Expect = 1.947e-10
Identity = 35/102 (34.31%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  VK L+    D+   +     P+H AC  G   +V  LID
Sbjct:  103 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELID 204          
BLAST of SMED30003476 vs. Ensembl Human
Match: ANKRD44 (ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:HGNC:25259])

HSP 1 Score: 66.2402 bits (160), Expect = 1.546e-13
Identity = 33/107 (30.84%), Postives = 57/107 (53.27%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI H  +++A  K  Q P+H A     ++   ++I    ++ + D      +HHA   GH+E V LL+    +I A D+ D R +H+A   GH+ +V LLI++  ++
Sbjct:   93 LIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEV 199          

HSP 2 Score: 64.3142 bits (155), Expect = 7.257e-13
Identity = 31/102 (30.39%), Postives = 55/102 (53.92%), Query Frame = 1
Query:   16 CNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
             +++   +  +  +H A + GH+E VN+L+ +  NI   D  D R +H A   GH++ V LLI H  ++   D+  + P+H A   G + +VK L++   +I
Sbjct:  131 SSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQINVVKHLLNLGVEI 232          

HSP 3 Score: 55.4546 bits (132), Expect = 1.166e-9
Identity = 33/107 (30.84%), Postives = 53/107 (49.53%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NI+A  K D+R +H+A   GH++ V +LI     +   D   +  +H A S G I  VK L+    +I+ ++      +H AC  G   +V  LIDY  ++
Sbjct:  159 LLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQINVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANV 265          

HSP 4 Score: 48.9062 bits (115), Expect = 2.358e-7
Identity = 26/95 (27.37%), Postives = 46/95 (48.42%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            +++ L    + P+H A   G  E + +LI     +   D      +H A ++   EAV++LI H  D+ A D+N   P+H A     +K  +++I
Sbjct:   33 DVNTLDSEKRTPLHVAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVII 127          

HSP 5 Score: 46.595 bits (109), Expect = 1.384e-6
Identity = 26/107 (24.30%), Postives = 46/107 (42.99%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    +    G +   P+H A +  H +    L+   + I+  D F    +H A + G++E +KLL     D    D+    P+HYA    H   ++ L+    ++
Sbjct:  358 LITSGADTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGFEIDTPDKFGRTCLHAAAAGGNVECIKLLQSSGADFHKKDKCGRTPLHYAAANCHFHCIETLVTTGANV 464          
BLAST of SMED30003476 vs. Ensembl Human
Match: ANKRD44 (ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:HGNC:25259])

HSP 1 Score: 66.2402 bits (160), Expect = 1.674e-13
Identity = 33/107 (30.84%), Postives = 57/107 (53.27%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI H  +++A  K  Q P+H A     ++   ++I    ++ + D      +HHA   GH+E V LL+    +I A D+ D R +H+A   GH+ +V LLI++  ++
Sbjct:   93 LIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEV 199          

HSP 2 Score: 64.3142 bits (155), Expect = 8.661e-13
Identity = 31/102 (30.39%), Postives = 55/102 (53.92%), Query Frame = 1
Query:   16 CNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
             +++   +  +  +H A + GH+E VN+L+ +  NI   D  D R +H A   GH++ V LLI H  ++   D+  + P+H A   G + +VK L++   +I
Sbjct:  131 SSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQINVVKHLLNLGVEI 232          

HSP 3 Score: 55.0694 bits (131), Expect = 1.433e-9
Identity = 33/107 (30.84%), Postives = 53/107 (49.53%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NI+A  K D+R +H+A   GH++ V +LI     +   D   +  +H A S G I  VK L+    +I+ ++      +H AC  G   +V  LIDY  ++
Sbjct:  159 LLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQINVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANV 265          

HSP 4 Score: 51.2174 bits (121), Expect = 3.007e-8
Identity = 26/93 (27.96%), Postives = 44/93 (47.31%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGH 279
            + D+   +D      Q P+  A   GHI+ V++L+++  N++ +D      +H     GH E V++L+     I   D     P+HYA   GH
Sbjct:  656 IADNPEAVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDTVDILGCTALHRGIMTGHEECVQMLLEQEVSILCKDSRGRTPLHYAAARGH 748          

HSP 5 Score: 48.9062 bits (115), Expect = 2.228e-7
Identity = 26/95 (27.37%), Postives = 46/95 (48.42%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            +++ L    + P+H A   G  E + +LI     +   D      +H A ++   EAV++LI H  D+ A D+N   P+H A     +K  +++I
Sbjct:   33 DVNTLDSEKRTPLHVAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVII 127          

HSP 6 Score: 46.595 bits (109), Expect = 1.557e-6
Identity = 26/107 (24.30%), Postives = 46/107 (42.99%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    +    G +   P+H A +  H +    L+   + I+  D F    +H A + G++E +KLL     D    D+    P+HYA    H   ++ L+    ++
Sbjct:  358 LITSGADTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGFEIDTPDKFGRTCLHAAAAGGNVECIKLLQSSGADFHKKDKCGRTPLHYAAANCHFHCIETLVTTGANV 464          

HSP 7 Score: 45.0542 bits (105), Expect = 4.364e-6
Identity = 24/88 (27.27%), Postives = 42/88 (47.73%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYH-HCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            + P+H A  A H+E + +L++    +  +D      +  A   G   AV +L+     D+   D++   P+H AC  GH K   L++D
Sbjct:  840 RTPLHAAAFADHVECLQLLLRHSAPVNAVDNSGKTALMMAAENGQAGAVDILVNSAQADLTVKDKDLNTPLHLACSKGHEKCALLILD 927          
BLAST of SMED30003476 vs. Ensembl Celegans
Match: trp-4 (TRP (Transient receptor potential) channel family [Source:UniProtKB/TrEMBL;Acc:Q9GRV5])

HSP 1 Score: 68.5514 bits (166), Expect = 1.243e-14
Identity = 36/92 (39.13%), Postives = 48/92 (52.17%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWR---LIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            P+H A   GHI  V +L+    + +     DWR    +H A   GH E V LLI    +I  +DQN W  +H+A  AGH+ +VKL ID   D
Sbjct: 1242 PLHLAAQQGHIAVVGMLLSR--STQQQHAKDWRGRTPLHLAAQNGHYEMVSLLIAQGSNINVMDQNGWTGLHFATRAGHLSVVKLFIDSSAD 1331          

HSP 2 Score: 56.9954 bits (136), Expect = 1.330e-10
Identity = 28/87 (32.18%), Postives = 44/87 (50.57%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYH 312
            P+  AC  GH    NIL+K +  I++ D      +H A   GH+  V LL+ H   + +  +    P+H A   GH+K+V +L+  H
Sbjct:  890 PLLEACARGHSGVANILLKHHARIDVFDEMGRTALHLAAFNGHLSLVHLLLQHKAFVNSKSKTGEAPLHLAAQHGHVKVVNVLVQDH 976          

HSP 3 Score: 55.8398 bits (133), Expect = 2.784e-10
Identity = 28/84 (33.33%), Postives = 44/84 (52.38%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            P+H A   GH E V++LI +  NI ++D   W  +H A  AGH+  VKL I    D  A  +    P+ +A    H++ ++ L+
Sbjct: 1276 PLHLAAQNGHYEMVSLLIAQGSNINVMDQNGWTGLHFATRAGHLSVVKLFIDSSADPLAETKEGKVPLCFAAAHNHIECLRFLL 1359          

HSP 4 Score: 45.8246 bits (107), Expect = 9.169e-7
Identity = 24/102 (23.53%), Postives = 49/102 (48.04%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHH-CDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            L+ HH  ID   +  +  +H A   GH+  V++L++    +          +H A   GH++ V +L+  H   +EA+  ++   +H+A   G + + + L+
Sbjct:  906 LLKHHARIDVFDEMGRTALHLAAFNGHLSLVHLLLQHKAFVNSKSKTGEAPLHLAAQHGHVKVVNVLVQDHGAALEAITLDNQTALHFAAKFGQLAVSQTLL 1007          
BLAST of SMED30003476 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 53.5286 bits (127), Expect = 1.925e-9
Identity = 28/99 (28.28%), Postives = 50/99 (50.51%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            ID+  K    P+H A  +GH + V++L+ +   I          +H A    H++A + L+YH   ++ +  +   P+H A   GH+++ KLL+D   D
Sbjct:  286 IDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSAD 384          

HSP 2 Score: 47.7506 bits (112), Expect = 1.971e-7
Identity = 27/102 (26.47%), Postives = 52/102 (50.98%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            LI +   +DA  +  Q P+H A   G+ + V +L++   N       ++  +H A   G  E   +L+ H+ D   L +  + P+H A   G++++V+LL++
Sbjct:  477 LIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLE 578          

HSP 3 Score: 45.8246 bits (107), Expect = 1.101e-6
Identity = 30/102 (29.41%), Postives = 47/102 (46.08%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILD-GFDWRL-IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            I A  K    P+H A    H++    L+  Y+   + D   D+   +H A   GH+   KLL+    D  +   N + P+H AC    +K+V+LL+ Y   I
Sbjct:  319 ISAKTKNGLAPLHMAAQGDHVDAARTLL--YHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAI 418          
BLAST of SMED30003476 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 53.5286 bits (127), Expect = 1.925e-9
Identity = 28/99 (28.28%), Postives = 50/99 (50.51%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            ID+  K    P+H A  +GH + V++L+ +   I          +H A    H++A + L+YH   ++ +  +   P+H A   GH+++ KLL+D   D
Sbjct:  286 IDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSAD 384          

HSP 2 Score: 47.7506 bits (112), Expect = 1.971e-7
Identity = 27/102 (26.47%), Postives = 52/102 (50.98%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            LI +   +DA  +  Q P+H A   G+ + V +L++   N       ++  +H A   G  E   +L+ H+ D   L +  + P+H A   G++++V+LL++
Sbjct:  477 LIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLE 578          

HSP 3 Score: 45.8246 bits (107), Expect = 1.101e-6
Identity = 30/102 (29.41%), Postives = 47/102 (46.08%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILD-GFDWRL-IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            I A  K    P+H A    H++    L+  Y+   + D   D+   +H A   GH+   KLL+    D  +   N + P+H AC    +K+V+LL+ Y   I
Sbjct:  319 ISAKTKNGLAPLHMAAQGDHVDAARTLL--YHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAI 418          
BLAST of SMED30003476 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 53.5286 bits (127), Expect = 1.926e-9
Identity = 28/99 (28.28%), Postives = 50/99 (50.51%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            ID+  K    P+H A  +GH + V++L+ +   I          +H A    H++A + L+YH   ++ +  +   P+H A   GH+++ KLL+D   D
Sbjct:  286 IDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSAD 384          

HSP 2 Score: 47.7506 bits (112), Expect = 1.971e-7
Identity = 27/102 (26.47%), Postives = 52/102 (50.98%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            LI +   +DA  +  Q P+H A   G+ + V +L++   N       ++  +H A   G  E   +L+ H+ D   L +  + P+H A   G++++V+LL++
Sbjct:  477 LIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLE 578          

HSP 3 Score: 45.8246 bits (107), Expect = 1.101e-6
Identity = 30/102 (29.41%), Postives = 47/102 (46.08%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILD-GFDWRL-IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            I A  K    P+H A    H++    L+  Y+   + D   D+   +H A   GH+   KLL+    D  +   N + P+H AC    +K+V+LL+ Y   I
Sbjct:  319 ISAKTKNGLAPLHMAAQGDHVDAARTLL--YHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAI 418          
BLAST of SMED30003476 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86])

HSP 1 Score: 53.5286 bits (127), Expect = 1.926e-9
Identity = 28/99 (28.28%), Postives = 50/99 (50.51%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            ID+  K    P+H A  +GH + V++L+ +   I          +H A    H++A + L+YH   ++ +  +   P+H A   GH+++ KLL+D   D
Sbjct:  286 IDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSAD 384          

HSP 2 Score: 47.7506 bits (112), Expect = 1.971e-7
Identity = 27/102 (26.47%), Postives = 52/102 (50.98%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            LI +   +DA  +  Q P+H A   G+ + V +L++   N       ++  +H A   G  E   +L+ H+ D   L +  + P+H A   G++++V+LL++
Sbjct:  477 LIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLE 578          

HSP 3 Score: 45.8246 bits (107), Expect = 1.101e-6
Identity = 30/102 (29.41%), Postives = 47/102 (46.08%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILD-GFDWRL-IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            I A  K    P+H A    H++    L+  Y+   + D   D+   +H A   GH+   KLL+    D  +   N + P+H AC    +K+V+LL+ Y   I
Sbjct:  319 ISAKTKNGLAPLHMAAQGDHVDAARTLL--YHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAI 418          
BLAST of SMED30003476 vs. Ensembl Fly
Match: nompC (gene:FBgn0016920 transcript:FBtr0300012)

HSP 1 Score: 65.855 bits (159), Expect = 1.402e-13
Identity = 33/88 (37.50%), Postives = 51/88 (57.95%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRL---IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            P+H AC  GH+  V +L+    + E+L   D      +H A   GHI+ V++L+    +I A D+N W P+H A  AGH+++VKLL +
Sbjct: 1013 PLHLACFGGHMSVVGLLLSR--SAELLQSQDRNGRTGLHIAAMHGHIQMVEILLGQGAEINATDRNGWTPLHCAAKAGHLEVVKLLCE 1098          

HSP 2 Score: 47.7506 bits (112), Expect = 2.946e-7
Identity = 28/102 (27.45%), Postives = 48/102 (47.06%), Query Frame = 1
Query:    7 DHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCD-IEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            DH+  ID L    Q P+H A  +G +E   +L++   NI+  D    + IH A    + E  KL +  H   + A  ++     H A   G +K+++ L+ +
Sbjct:  748 DHNAVIDILTLRKQTPLHLAAASGQMEVCQLLLELGANIDATDDLGQKPIHVAAQNNYSEVAKLFLQQHPSLVNATSKDGNTCAHIAAMQGSVKVIEELMKF 849          

HSP 3 Score: 43.8986 bits (102), Expect = 5.333e-6
Identity = 28/105 (26.67%), Postives = 52/105 (49.52%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYN-IEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYH 312
            L+ +   I++  +  +  +H A + G    V  LIK++   I+IL       +H A ++G +E  +LL+    +I+A D    +PIH A    + ++ KL +  H
Sbjct:  712 LLTNKAFINSKSRVGRTALHLAAMNGFTHLVKFLIKDHNAVIDILTLRKQTPLHLAAASGQMEVCQLLLELGANIDATDDLGQKPIHVAAQNNYSEVAKLFLQQH 816          
BLAST of SMED30003476 vs. Ensembl Fly
Match: nompC (gene:FBgn0016920 transcript:FBtr0304843)

HSP 1 Score: 65.855 bits (159), Expect = 1.402e-13
Identity = 33/88 (37.50%), Postives = 51/88 (57.95%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRL---IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            P+H AC  GH+  V +L+    + E+L   D      +H A   GHI+ V++L+    +I A D+N W P+H A  AGH+++VKLL +
Sbjct: 1013 PLHLACFGGHMSVVGLLLSR--SAELLQSQDRNGRTGLHIAAMHGHIQMVEILLGQGAEINATDRNGWTPLHCAAKAGHLEVVKLLCE 1098          

HSP 2 Score: 47.7506 bits (112), Expect = 2.946e-7
Identity = 28/102 (27.45%), Postives = 48/102 (47.06%), Query Frame = 1
Query:    7 DHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCD-IEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            DH+  ID L    Q P+H A  +G +E   +L++   NI+  D    + IH A    + E  KL +  H   + A  ++     H A   G +K+++ L+ +
Sbjct:  748 DHNAVIDILTLRKQTPLHLAAASGQMEVCQLLLELGANIDATDDLGQKPIHVAAQNNYSEVAKLFLQQHPSLVNATSKDGNTCAHIAAMQGSVKVIEELMKF 849          

HSP 3 Score: 43.8986 bits (102), Expect = 5.334e-6
Identity = 28/105 (26.67%), Postives = 52/105 (49.52%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYN-IEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYH 312
            L+ +   I++  +  +  +H A + G    V  LIK++   I+IL       +H A ++G +E  +LL+    +I+A D    +PIH A    + ++ KL +  H
Sbjct:  712 LLTNKAFINSKSRVGRTALHLAAMNGFTHLVKFLIKDHNAVIDILTLRKQTPLHLAAASGQMEVCQLLLELGANIDATDDLGQKPIHVAAQNNYSEVAKLFLQQH 816          
BLAST of SMED30003476 vs. Ensembl Fly
Match: nompC (gene:FBgn0016920 transcript:FBtr0343224)

HSP 1 Score: 65.855 bits (159), Expect = 1.402e-13
Identity = 33/88 (37.50%), Postives = 51/88 (57.95%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRL---IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            P+H AC  GH+  V +L+    + E+L   D      +H A   GHI+ V++L+    +I A D+N W P+H A  AGH+++VKLL +
Sbjct: 1013 PLHLACFGGHMSVVGLLLSR--SAELLQSQDRNGRTGLHIAAMHGHIQMVEILLGQGAEINATDRNGWTPLHCAAKAGHLEVVKLLCE 1098          

HSP 2 Score: 47.7506 bits (112), Expect = 2.946e-7
Identity = 28/102 (27.45%), Postives = 48/102 (47.06%), Query Frame = 1
Query:    7 DHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCD-IEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            DH+  ID L    Q P+H A  +G +E   +L++   NI+  D    + IH A    + E  KL +  H   + A  ++     H A   G +K+++ L+ +
Sbjct:  748 DHNAVIDILTLRKQTPLHLAAASGQMEVCQLLLELGANIDATDDLGQKPIHVAAQNNYSEVAKLFLQQHPSLVNATSKDGNTCAHIAAMQGSVKVIEELMKF 849          

HSP 3 Score: 43.8986 bits (102), Expect = 5.334e-6
Identity = 28/105 (26.67%), Postives = 52/105 (49.52%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYN-IEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYH 312
            L+ +   I++  +  +  +H A + G    V  LIK++   I+IL       +H A ++G +E  +LL+    +I+A D    +PIH A    + ++ KL +  H
Sbjct:  712 LLTNKAFINSKSRVGRTALHLAAMNGFTHLVKFLIKDHNAVIDILTLRKQTPLHLAAASGQMEVCQLLLELGANIDATDDLGQKPIHVAAQNNYSEVAKLFLQQH 816          
BLAST of SMED30003476 vs. Ensembl Fly
Match: nompC (gene:FBgn0016920 transcript:FBtr0347408)

HSP 1 Score: 65.855 bits (159), Expect = 1.402e-13
Identity = 33/88 (37.50%), Postives = 51/88 (57.95%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRL---IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            P+H AC  GH+  V +L+    + E+L   D      +H A   GHI+ V++L+    +I A D+N W P+H A  AGH+++VKLL +
Sbjct: 1013 PLHLACFGGHMSVVGLLLSR--SAELLQSQDRNGRTGLHIAAMHGHIQMVEILLGQGAEINATDRNGWTPLHCAAKAGHLEVVKLLCE 1098          

HSP 2 Score: 47.7506 bits (112), Expect = 2.947e-7
Identity = 28/102 (27.45%), Postives = 48/102 (47.06%), Query Frame = 1
Query:    7 DHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCD-IEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            DH+  ID L    Q P+H A  +G +E   +L++   NI+  D    + IH A    + E  KL +  H   + A  ++     H A   G +K+++ L+ +
Sbjct:  748 DHNAVIDILTLRKQTPLHLAAASGQMEVCQLLLELGANIDATDDLGQKPIHVAAQNNYSEVAKLFLQQHPSLVNATSKDGNTCAHIAAMQGSVKVIEELMKF 849          

HSP 3 Score: 43.8986 bits (102), Expect = 5.336e-6
Identity = 28/105 (26.67%), Postives = 52/105 (49.52%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYN-IEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYH 312
            L+ +   I++  +  +  +H A + G    V  LIK++   I+IL       +H A ++G +E  +LL+    +I+A D    +PIH A    + ++ KL +  H
Sbjct:  712 LLTNKAFINSKSRVGRTALHLAAMNGFTHLVKFLIKDHNAVIDILTLRKQTPLHLAAASGQMEVCQLLLELGANIDATDDLGQKPIHVAAQNNYSEVAKLFLQQH 816          
BLAST of SMED30003476 vs. Ensembl Fly
Match: nompC (gene:FBgn0016920 transcript:FBtr0347409)

HSP 1 Score: 65.855 bits (159), Expect = 1.403e-13
Identity = 33/88 (37.50%), Postives = 51/88 (57.95%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRL---IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            P+H AC  GH+  V +L+    + E+L   D      +H A   GHI+ V++L+    +I A D+N W P+H A  AGH+++VKLL +
Sbjct: 1013 PLHLACFGGHMSVVGLLLSR--SAELLQSQDRNGRTGLHIAAMHGHIQMVEILLGQGAEINATDRNGWTPLHCAAKAGHLEVVKLLCE 1098          

HSP 2 Score: 47.7506 bits (112), Expect = 2.947e-7
Identity = 28/102 (27.45%), Postives = 48/102 (47.06%), Query Frame = 1
Query:    7 DHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCD-IEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            DH+  ID L    Q P+H A  +G +E   +L++   NI+  D    + IH A    + E  KL +  H   + A  ++     H A   G +K+++ L+ +
Sbjct:  748 DHNAVIDILTLRKQTPLHLAAASGQMEVCQLLLELGANIDATDDLGQKPIHVAAQNNYSEVAKLFLQQHPSLVNATSKDGNTCAHIAAMQGSVKVIEELMKF 849          

HSP 3 Score: 43.8986 bits (102), Expect = 5.337e-6
Identity = 28/105 (26.67%), Postives = 52/105 (49.52%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYN-IEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYH 312
            L+ +   I++  +  +  +H A + G    V  LIK++   I+IL       +H A ++G +E  +LL+    +I+A D    +PIH A    + ++ KL +  H
Sbjct:  712 LLTNKAFINSKSRVGRTALHLAAMNGFTHLVKFLIKDHNAVIDILTLRKQTPLHLAAASGQMEVCQLLLELGANIDATDDLGQKPIHVAAQNNYSEVAKLFLQQH 816          
BLAST of SMED30003476 vs. Ensembl Zebrafish
Match: ankrd28b (ankyrin repeat domain 28b [Source:ZFIN;Acc:ZDB-GENE-070622-4])

HSP 1 Score: 69.707 bits (169), Expect = 6.883e-15
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     +     L+    N+ + D      +HHA  +GH+E V+LL+    +I A D+ D R IH+A   GHM++VKLL+ +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHVAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGANINAFDKKDRRAIHWAAYMGHMEVVKLLVSHGAEV 199          

HSP 2 Score: 68.9366 bits (167), Expect = 1.484e-14
Identity = 34/101 (33.66%), Postives = 55/101 (54.46%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH+E V +L+    NI   D  D R IH A   GH+E VKLL+ H  ++   D+  + P+H A  +G + +VK L+D   D+
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGANINAFDKKDRRAIHWAAYMGHMEVVKLLVSHGAEVPCKDKKAYTPLHAAASSGMISVVKYLLDMGVDM 232          

HSP 3 Score: 54.6842 bits (130), Expect = 1.285e-9
Identity = 33/102 (32.35%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GH+E V +L+     +   D   +  +H A S+G I  VK L+    D+   +     P+H AC  G   +V  LI+
Sbjct:  159 LLSRGANINAFDKKDRRAIHWAAYMGHMEVVKLLVSHGAEVPCKDKKAYTPLHAAASSGMISVVKYLLDMGVDMNEPNAYGNTPLHVACYNGQDVVVNELIE 260          

HSP 4 Score: 49.6766 bits (117), Expect = 6.533e-8
Identity = 28/106 (26.42%), Postives = 54/106 (50.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEAL----DQNDWRPIHYACCAGHMKIVKLLID 306
            LI+   N+DA  K+ +  +H   + GH E V  L++   +  + D      +H A + GH+  +  L++    +E++    D   + P+H+AC  GH   V++L++
Sbjct:  672 LINKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHSASFMVRDCRGRSPVHLASACGHVGVLGGLLHAAQSVESIPVITDHQGYTPLHWACYNGHDTCVEVLLE 777          
BLAST of SMED30003476 vs. Ensembl Zebrafish
Match: ankrd28b (ankyrin repeat domain 28b [Source:ZFIN;Acc:ZDB-GENE-070622-4])

HSP 1 Score: 69.707 bits (169), Expect = 6.883e-15
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     +     L+    N+ + D      +HHA  +GH+E V+LL+    +I A D+ D R IH+A   GHM++VKLL+ +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHVAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGANINAFDKKDRRAIHWAAYMGHMEVVKLLVSHGAEV 199          

HSP 2 Score: 68.9366 bits (167), Expect = 1.484e-14
Identity = 34/101 (33.66%), Postives = 55/101 (54.46%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH+E V +L+    NI   D  D R IH A   GH+E VKLL+ H  ++   D+  + P+H A  +G + +VK L+D   D+
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGANINAFDKKDRRAIHWAAYMGHMEVVKLLVSHGAEVPCKDKKAYTPLHAAASSGMISVVKYLLDMGVDM 232          

HSP 3 Score: 54.6842 bits (130), Expect = 1.285e-9
Identity = 33/102 (32.35%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GH+E V +L+     +   D   +  +H A S+G I  VK L+    D+   +     P+H AC  G   +V  LI+
Sbjct:  159 LLSRGANINAFDKKDRRAIHWAAYMGHMEVVKLLVSHGAEVPCKDKKAYTPLHAAASSGMISVVKYLLDMGVDMNEPNAYGNTPLHVACYNGQDVVVNELIE 260          

HSP 4 Score: 49.6766 bits (117), Expect = 6.533e-8
Identity = 28/106 (26.42%), Postives = 54/106 (50.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEAL----DQNDWRPIHYACCAGHMKIVKLLID 306
            LI+   N+DA  K+ +  +H   + GH E V  L++   +  + D      +H A + GH+  +  L++    +E++    D   + P+H+AC  GH   V++L++
Sbjct:  672 LINKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHSASFMVRDCRGRSPVHLASACGHVGVLGGLLHAAQSVESIPVITDHQGYTPLHWACYNGHDTCVEVLLE 777          
BLAST of SMED30003476 vs. Ensembl Zebrafish
Match: ankrd16 (ankyrin repeat domain 16 [Source:ZFIN;Acc:ZDB-GENE-041210-253])

HSP 1 Score: 61.6178 bits (148), Expect = 4.983e-12
Identity = 30/97 (30.93%), Postives = 54/97 (55.67%), Query Frame = 1
Query:   31 LGKYDQRPIHFACIAGHIETVNILIKEY-YNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
             GK     +H+A   GH++ +  L++E   +IE+ +    R +H A S GH E V+ LI     +++L + DW P+  AC   ++++++ L+DY  D
Sbjct:   41 FGKSGDTVLHYAARHGHVDILRYLVEELNMDIEVYNSDYKRALHEASSMGHYECVRFLICRGAKVDSLKKADWTPLMMACTRRNLEVIQELLDYGAD 137          

HSP 2 Score: 45.4394 bits (106), Expect = 2.327e-6
Identity = 26/92 (28.26%), Postives = 43/92 (46.74%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            +R +H A   GH E V  LI     ++ L   DW  +  AC+  ++E ++ L+ +  D    +++ W   H AC  G   I+  L+    DI
Sbjct:   80 KRALHEASSMGHYECVRFLICRGAKVDSLKKADWTPLMMACTRRNLEVIQELLDYGADPTLQNKDGWSSFHIACREGDPAIINHLLLAKPDI 171          
BLAST of SMED30003476 vs. Ensembl Zebrafish
Match: ankrd16 (ankyrin repeat domain 16 [Source:ZFIN;Acc:ZDB-GENE-041210-253])

HSP 1 Score: 61.2326 bits (147), Expect = 5.131e-12
Identity = 30/97 (30.93%), Postives = 54/97 (55.67%), Query Frame = 1
Query:   31 LGKYDQRPIHFACIAGHIETVNILIKEY-YNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
             GK     +H+A   GH++ +  L++E   +IE+ +    R +H A S GH E V+ LI     +++L + DW P+  AC   ++++++ L+DY  D
Sbjct:   41 FGKSGDTVLHYAARHGHVDILRYLVEELNMDIEVYNSDYKRALHEASSMGHYECVRFLICRGAKVDSLKKADWTPLMMACTRRNLEVIQELLDYGAD 137          

HSP 2 Score: 45.0542 bits (105), Expect = 2.641e-6
Identity = 26/92 (28.26%), Postives = 43/92 (46.74%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            +R +H A   GH E V  LI     ++ L   DW  +  AC+  ++E ++ L+ +  D    +++ W   H AC  G   I+  L+    DI
Sbjct:   80 KRALHEASSMGHYECVRFLICRGAKVDSLKKADWTPLMMACTRRNLEVIQELLDYGADPTLQNKDGWSSFHIACREGDPAIINHLLLAKPDI 171          
BLAST of SMED30003476 vs. Ensembl Zebrafish
Match: ankrd52a (ankyrin repeat domain 52a [Source:NCBI gene;Acc:553206])

HSP 1 Score: 60.4622 bits (145), Expect = 1.410e-11
Identity = 34/106 (32.08%), Postives = 55/106 (51.89%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            L+    ++ A  KY Q P+H A        V  L+    ++ + D      +HHA  +G+ E VKLL+    ++ A D+ D +PIH+A   GH+++VKLL+    D
Sbjct:   93 LLRKGADVTARDKYWQTPLHIAAANRATRCVETLLPHVSSLNMADRTGRAPLHHAAQSGYQEMVKLLLNKGANLSASDKKDRQPIHWAAYLGHLEVVKLLVSQGSD 198          

HSP 2 Score: 60.077 bits (144), Expect = 1.524e-11
Identity = 32/101 (31.68%), Postives = 55/101 (54.46%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            L+ H  +++   +  + P+H A  +G+ E V +L+ +  N+   D  D + IH A   GH+E VKLL+    D    D+  + P+H A  +GH+ +VK L+
Sbjct:  126 LLPHVSSLNMADRTGRAPLHHAAQSGYQEMVKLLLNKGANLSASDKKDRQPIHWAAYLGHLEVVKLLVSQGSDKSCKDKRGYTPLHAAAASGHVDVVKYLL 226          

HSP 3 Score: 53.5286 bits (127), Expect = 3.144e-9
Identity = 28/102 (27.45%), Postives = 54/102 (52.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+ A  K D++PIH+A   GH+E V +L+ +  +    D   +  +H A ++GH++ VK L+ +  +I+  +      +H AC  G   +   L++
Sbjct:  159 LLNKGANLSASDKKDRQPIHWAAYLGHLEVVKLLVSQGSDKSCKDKRGYTPLHAAAASGHVDVVKYLLRNGAEIDEPNAFGNTALHVACYTGQEAVANELVN 260          

HSP 4 Score: 44.669 bits (104), Expect = 4.149e-6
Identity = 24/85 (28.24%), Postives = 45/85 (52.94%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCD-IEALDQNDWRPIHYACCAGHMKIVKLLI 303
            P+H A  +GH E V +LI+    +++ D     +++ A   GH   V+LL+      + A  ++ W P+H A   GH + +++L+
Sbjct:  568 PLHLAVESGHWECVTVLIESGACVDVCDPVGRSVLYLASQRGHSRCVELLLSQSASCLLAEHRSKWGPLHVAAANGHSECLRMLL 652          
BLAST of SMED30003476 vs. Ensembl Xenopus
Match: rev1 (REV1, DNA directed polymerase [Source:Xenbase;Acc:XB-GENE-968197])

HSP 1 Score: 70.0922 bits (170), Expect = 6.465e-15
Identity = 34/101 (33.66%), Postives = 56/101 (55.45%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH+E V++L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + ++K L+D   D+
Sbjct:  159 NVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDM 259          

HSP 2 Score: 67.781 bits (164), Expect = 5.231e-14
Identity = 34/107 (31.78%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH+E V LL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:  120 LLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEV 226          

HSP 3 Score: 56.6102 bits (135), Expect = 4.100e-10
Identity = 34/102 (33.33%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  +K L+    D+   +     P+H AC  G   +V  LID
Sbjct:  186 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYNGQDVVVNELID 287          

HSP 4 Score: 50.8322 bits (120), Expect = 4.119e-8
Identity = 30/106 (28.30%), Postives = 53/106 (50.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEAL----DQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V  L++   N  + D      IH A + GHI  +  L+     ++ +    D + + P+H+AC  GH   V+LL++
Sbjct:  699 LLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLE 804          

HSP 5 Score: 45.0542 bits (105), Expect = 4.465e-6
Identity = 24/86 (27.91%), Postives = 41/86 (47.67%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            Q P+  + + GH E V  L+ +  N++  D +    +H     GH E V+ L+ H+ +    D     PIH A   GH+ ++  L+
Sbjct:  681 QTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALL 766          
BLAST of SMED30003476 vs. Ensembl Xenopus
Match: rev1 (REV1, DNA directed polymerase [Source:Xenbase;Acc:XB-GENE-968197])

HSP 1 Score: 70.0922 bits (170), Expect = 7.263e-15
Identity = 34/101 (33.66%), Postives = 56/101 (55.45%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH+E V++L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + ++K L+D   D+
Sbjct:  151 NVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDM 251          

HSP 2 Score: 67.3958 bits (163), Expect = 5.763e-14
Identity = 34/107 (31.78%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH+E V LL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:  112 LLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEV 218          

HSP 3 Score: 56.6102 bits (135), Expect = 4.430e-10
Identity = 34/102 (33.33%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  +K L+    D+   +     P+H AC  G   +V  LID
Sbjct:  178 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYNGQDVVVNELID 279          

HSP 4 Score: 50.8322 bits (120), Expect = 4.450e-8
Identity = 30/106 (28.30%), Postives = 53/106 (50.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEAL----DQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V  L++   N  + D      IH A + GHI  +  L+     ++ +    D + + P+H+AC  GH   V+LL++
Sbjct:  691 LLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLE 796          

HSP 5 Score: 45.0542 bits (105), Expect = 4.777e-6
Identity = 24/86 (27.91%), Postives = 41/86 (47.67%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            Q P+  + + GH E V  L+ +  N++  D +    +H     GH E V+ L+ H+ +    D     PIH A   GH+ ++  L+
Sbjct:  673 QTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALL 758          
BLAST of SMED30003476 vs. Ensembl Xenopus
Match: rev1 (REV1, DNA directed polymerase [Source:Xenbase;Acc:XB-GENE-968197])

HSP 1 Score: 70.0922 bits (170), Expect = 7.327e-15
Identity = 34/101 (33.66%), Postives = 56/101 (55.45%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH+E V++L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + ++K L+D   D+
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDM 232          

HSP 2 Score: 67.3958 bits (163), Expect = 5.928e-14
Identity = 34/107 (31.78%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH+E V LL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEV 199          

HSP 3 Score: 56.6102 bits (135), Expect = 4.469e-10
Identity = 34/102 (33.33%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  +K L+    D+   +     P+H AC  G   +V  LID
Sbjct:  159 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYNGQDVVVNELID 260          

HSP 4 Score: 50.8322 bits (120), Expect = 4.533e-8
Identity = 30/106 (28.30%), Postives = 53/106 (50.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEAL----DQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V  L++   N  + D      IH A + GHI  +  L+     ++ +    D + + P+H+AC  GH   V+LL++
Sbjct:  672 LLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLE 777          

HSP 5 Score: 45.0542 bits (105), Expect = 4.914e-6
Identity = 24/86 (27.91%), Postives = 41/86 (47.67%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            Q P+  + + GH E V  L+ +  N++  D +    +H     GH E V+ L+ H+ +    D     PIH A   GH+ ++  L+
Sbjct:  654 QTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALL 739          
BLAST of SMED30003476 vs. Ensembl Xenopus
Match: rev1 (REV1, DNA directed polymerase [Source:Xenbase;Acc:XB-GENE-968197])

HSP 1 Score: 70.0922 bits (170), Expect = 7.398e-15
Identity = 34/101 (33.66%), Postives = 56/101 (55.45%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH+E V++L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + ++K L+D   D+
Sbjct:  159 NVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDM 259          

HSP 2 Score: 67.3958 bits (163), Expect = 5.927e-14
Identity = 34/107 (31.78%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH+E V LL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:  120 LLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEV 226          

HSP 3 Score: 56.6102 bits (135), Expect = 4.601e-10
Identity = 34/102 (33.33%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  +K L+    D+   +     P+H AC  G   +V  LID
Sbjct:  186 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYNGQDVVVNELID 287          

HSP 4 Score: 50.8322 bits (120), Expect = 4.489e-8
Identity = 30/106 (28.30%), Postives = 53/106 (50.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEAL----DQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V  L++   N  + D      IH A + GHI  +  L+     ++ +    D + + P+H+AC  GH   V+LL++
Sbjct:  699 LLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLE 804          

HSP 5 Score: 45.0542 bits (105), Expect = 4.819e-6
Identity = 24/86 (27.91%), Postives = 41/86 (47.67%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            Q P+  + + GH E V  L+ +  N++  D +    +H     GH E V+ L+ H+ +    D     PIH A   GH+ ++  L+
Sbjct:  681 QTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGAVTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALL 766          
BLAST of SMED30003476 vs. Ensembl Xenopus
Match: rev1 (REV1, DNA directed polymerase [Source:Xenbase;Acc:XB-GENE-968197])

HSP 1 Score: 69.707 bits (169), Expect = 8.814e-15
Identity = 34/101 (33.66%), Postives = 56/101 (55.45%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH+E V++L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + ++K L+D   D+
Sbjct:  130 NVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDM 230          

HSP 2 Score: 67.3958 bits (163), Expect = 6.926e-14
Identity = 34/107 (31.78%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH+E V LL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:   91 LLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHVEMVSLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEV 197          

HSP 3 Score: 56.225 bits (134), Expect = 5.171e-10
Identity = 34/102 (33.33%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  +K L+    D+   +     P+H AC  G   +V  LID
Sbjct:  157 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVTHGAEVMCKDKKSYTPLHAAASSGMISVIKYLLDLGVDMNESNAYGNTPLHVACYNGQDVVVNELID 258          

HSP 4 Score: 50.0618 bits (118), Expect = 9.220e-8
Identity = 30/106 (28.30%), Postives = 53/106 (50.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEAL----DQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V  L++   N  + D      IH A + GHI  +  L+     ++ +    D + + P+H+AC  GH   V+LL++
Sbjct:  673 LLNKGANVDAKDKWGRTALHRGVMTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALLQTAISVDVVPAIADNHGYTPLHWACYNGHDACVELLLE 778          

HSP 5 Score: 44.669 bits (104), Expect = 5.969e-6
Identity = 24/86 (27.91%), Postives = 41/86 (47.67%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            Q P+  + + GH E V  L+ +  N++  D +    +H     GH E V+ L+ H+ +    D     PIH A   GH+ ++  L+
Sbjct:  655 QTPLMLSVLNGHTECVYSLLNKGANVDAKDKWGRTALHRGVMTGHEECVEALLQHNANFLLRDCRGRTPIHLAAACGHIGVLSALL 740          
BLAST of SMED30003476 vs. Ensembl Mouse
Match: Ankrd28 (ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MGI:2145661])

HSP 1 Score: 71.2478 bits (173), Expect = 2.266e-16
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH E VKLL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:   65 LLKHSADVNARDKNWQTPLHIAAANKAVKCAESLVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEV 171          

HSP 2 Score: 59.3066 bits (142), Expect = 1.017e-11
Identity = 29/83 (34.94%), Postives = 43/83 (51.81%), Query Frame = 1
Query:   16 CNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYA 264
             N++   +  +  +H A  +GH E V +L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A
Sbjct:  103 SNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAA 185          
BLAST of SMED30003476 vs. Ensembl Mouse
Match: Ankrd28 (ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MGI:2145661])

HSP 1 Score: 69.707 bits (169), Expect = 8.360e-15
Identity = 35/101 (34.65%), Postives = 54/101 (53.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH E V +L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + +VK L+D   D+
Sbjct:  162 NVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDM 262          

HSP 2 Score: 68.1662 bits (165), Expect = 2.765e-14
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH E VKLL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:  123 LLKHSADVNARDKNWQTPLHIAAANKAVKCAESLVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEV 229          

HSP 3 Score: 58.5362 bits (140), Expect = 6.363e-11
Identity = 35/102 (34.31%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  VK L+    D+   +     P+H AC  G   +V  LID
Sbjct:  189 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELID 290          

HSP 4 Score: 47.7506 bits (112), Expect = 3.723e-7
Identity = 28/106 (26.42%), Postives = 53/106 (50.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEA----LDQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V+ L++      + D      IH + + GHI  +  L+     ++A    +D + +  +H+AC  GH   V+LL++
Sbjct:  702 LLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSATSVDANPAVVDNHGYTALHWACYNGHETCVELLLE 807          
BLAST of SMED30003476 vs. Ensembl Mouse
Match: Ankrd28 (ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MGI:2145661])

HSP 1 Score: 69.3218 bits (168), Expect = 9.565e-15
Identity = 35/101 (34.65%), Postives = 54/101 (53.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH E V +L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + +VK L+D   D+
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDM 232          

HSP 2 Score: 67.781 bits (164), Expect = 3.225e-14
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH E VKLL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHIAAANKAVKCAESLVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEV 199          

HSP 3 Score: 58.5362 bits (140), Expect = 6.935e-11
Identity = 35/102 (34.31%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  VK L+    D+   +     P+H AC  G   +V  LID
Sbjct:  159 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELID 260          

HSP 4 Score: 47.7506 bits (112), Expect = 4.137e-7
Identity = 28/106 (26.42%), Postives = 53/106 (50.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEA----LDQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V+ L++      + D      IH + + GHI  +  L+     ++A    +D + +  +H+AC  GH   V+LL++
Sbjct:  672 LLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSATSVDANPAVVDNHGYTALHWACYNGHETCVELLLE 777          
BLAST of SMED30003476 vs. Ensembl Mouse
Match: Ankrd44 (ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MGI:3045243])

HSP 1 Score: 65.855 bits (159), Expect = 1.681e-13
Identity = 33/107 (30.84%), Postives = 57/107 (53.27%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI H  +++A  K  Q P+H A     ++   ++I    ++ + D      +HHA   GH+E V LL+    +I A D+ D R +H+A   GH+ +V LLI++  ++
Sbjct:   93 LIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGGRTALHHAALNGHMEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEV 199          

HSP 2 Score: 63.929 bits (154), Expect = 7.891e-13
Identity = 31/102 (30.39%), Postives = 55/102 (53.92%), Query Frame = 1
Query:   16 CNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
             +++   +  +  +H A + GH+E VN+L+ +  NI   D  D R +H A   GH++ V LLI H  ++   D+  + P+H A   G + +VK L++   +I
Sbjct:  131 SSVNVSDRGGRTALHHAALNGHMEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQISVVKHLLNLGVEI 232          

HSP 3 Score: 55.4546 bits (132), Expect = 8.675e-10
Identity = 33/107 (30.84%), Postives = 53/107 (49.53%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NI+A  K D+R +H+A   GH++ V +LI     +   D   +  +H A S G I  VK L+    +I+ ++      +H AC  G   +V  LIDY  ++
Sbjct:  159 LLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQISVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANV 265          

HSP 4 Score: 50.8322 bits (120), Expect = 3.493e-8
Identity = 25/86 (29.07%), Postives = 41/86 (47.67%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGH 279
            +D      Q P+  A   GHI+ V++L+++  N++ +D      +H     GH E V++L+     I   D     P+HYA   GH
Sbjct:  681 VDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDAVDIVGCTALHRGIMTGHEECVQMLLEQEASILCKDSRGRTPLHYAAARGH 766          

HSP 5 Score: 50.447 bits (119), Expect = 5.006e-8
Identity = 27/95 (28.42%), Postives = 47/95 (49.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            +++AL    + P+H A   G  E + +LI     +   D      +H A ++   EAV++LI H  D+ A D+N   P+H A     +K  +++I
Sbjct:   33 DVNALDSEKRTPLHVAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVII 127          

HSP 6 Score: 44.669 bits (104), Expect = 4.338e-6
Identity = 26/95 (27.37%), Postives = 50/95 (52.63%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWR---LIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            + P+H + I GH   + +L++   N E++D  D +    +  A + GHI+AV LL+    +++A+D      +H     GH + V++L++    I
Sbjct:  653 RTPLHASVINGHTLCLRLLLETADNPEVVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDAVDIVGCTALHRGIMTGHEECVQMLLEQEASI 747          
BLAST of SMED30003476 vs. Ensembl Mouse
Match: Ankrd44 (ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MGI:3045243])

HSP 1 Score: 65.855 bits (159), Expect = 1.763e-13
Identity = 33/107 (30.84%), Postives = 57/107 (53.27%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI H  +++A  K  Q P+H A     ++   ++I    ++ + D      +HHA   GH+E V LL+    +I A D+ D R +H+A   GH+ +V LLI++  ++
Sbjct:   93 LIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGGRTALHHAALNGHMEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEV 199          

HSP 2 Score: 63.929 bits (154), Expect = 8.689e-13
Identity = 31/102 (30.39%), Postives = 55/102 (53.92%), Query Frame = 1
Query:   16 CNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
             +++   +  +  +H A + GH+E VN+L+ +  NI   D  D R +H A   GH++ V LLI H  ++   D+  + P+H A   G + +VK L++   +I
Sbjct:  131 SSVNVSDRGGRTALHHAALNGHMEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQISVVKHLLNLGVEI 232          

HSP 3 Score: 55.0694 bits (131), Expect = 9.368e-10
Identity = 33/107 (30.84%), Postives = 53/107 (49.53%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NI+A  K D+R +H+A   GH++ V +LI     +   D   +  +H A S G I  VK L+    +I+ ++      +H AC  G   +V  LIDY  ++
Sbjct:  159 LLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQISVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANV 265          

HSP 4 Score: 50.8322 bits (120), Expect = 3.699e-8
Identity = 25/86 (29.07%), Postives = 41/86 (47.67%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGH 279
            +D      Q P+  A   GHI+ V++L+++  N++ +D      +H     GH E V++L+     I   D     P+HYA   GH
Sbjct:  663 VDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDAVDIVGCTALHRGIMTGHEECVQMLLEQEASILCKDSRGRTPLHYAAARGH 748          

HSP 5 Score: 50.447 bits (119), Expect = 5.050e-8
Identity = 27/95 (28.42%), Postives = 47/95 (49.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            +++AL    + P+H A   G  E + +LI     +   D      +H A ++   EAV++LI H  D+ A D+N   P+H A     +K  +++I
Sbjct:   33 DVNALDSEKRTPLHVAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVII 127          

HSP 6 Score: 47.7506 bits (112), Expect = 3.530e-7
Identity = 27/107 (25.23%), Postives = 46/107 (42.99%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    +    G +   P+H A +  H +    L+   + I+  D F    +H A + G++E +KLL     D    D+    P+HYA    H   +K L+    ++
Sbjct:  358 LITSGADTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGFEIDTPDTFGRTCLHAAAAGGNVECIKLLQSSGADFHKKDKCGRTPLHYAAANCHFHCIKALVTTGANV 464          

HSP 7 Score: 44.669 bits (104), Expect = 4.292e-6
Identity = 26/95 (27.37%), Postives = 50/95 (52.63%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWR---LIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            + P+H + I GH   + +L++   N E++D  D +    +  A + GHI+AV LL+    +++A+D      +H     GH + V++L++    I
Sbjct:  635 RTPLHASVINGHTLCLRLLLETADNPEVVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDAVDIVGCTALHRGIMTGHEECVQMLLEQEASI 729          
BLAST of SMED30003476 vs. UniProt/SwissProt
Match: sp|O15084|ANR28_HUMAN (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A OS=Homo sapiens OX=9606 GN=ANKRD28 PE=1 SV=5)

HSP 1 Score: 69.3218 bits (168), Expect = 6.499e-14
Identity = 35/101 (34.65%), Postives = 54/101 (53.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH E V +L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + +VK L+D   D+
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDM 232          

HSP 2 Score: 68.1662 bits (165), Expect = 1.621e-13
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH E VKLL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHIAAANKAVKCAEALVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEV 199          

HSP 3 Score: 58.5362 bits (140), Expect = 4.898e-10
Identity = 35/102 (34.31%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  VK L+    D+   +     P+H AC  G   +V  LID
Sbjct:  159 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELID 260          

HSP 4 Score: 46.2098 bits (108), Expect = 8.349e-6
Identity = 28/106 (26.42%), Postives = 52/106 (49.06%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEA----LDQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V+ L++      + D      IH + + GHI  +  L+     ++A     D + +  +H+AC  GH   V+LL++
Sbjct:  672 LLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSAASMDANPATADNHGYTALHWACYNGHETCVELLLE 777          
BLAST of SMED30003476 vs. UniProt/SwissProt
Match: sp|Q505D1|ANR28_MOUSE (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A OS=Mus musculus OX=10090 GN=Ankrd28 PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 6.691e-14
Identity = 35/101 (34.65%), Postives = 54/101 (53.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH E V +L+    NI   D  D R IH A   GHIE VKLL+ H  ++   D+  + P+H A  +G + +VK L+D   D+
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDM 232          

HSP 2 Score: 67.781 bits (164), Expect = 2.256e-13
Identity = 35/107 (32.71%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    L+    N+ + D      +HHA  +GH E VKLL+    +I A D+ D R IH+A   GH+++VKLL+ +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHIAAANKAVKCAESLVPLLSNVNVSDRAGRTALHHAAFSGHGEMVKLLLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEV 199          

HSP 3 Score: 58.5362 bits (140), Expect = 4.851e-10
Identity = 35/102 (34.31%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L+     +   D   +  +H A S+G I  VK L+    D+   +     P+H AC  G   +V  LID
Sbjct:  159 LLSRGANINAFDKKDRRAIHWAAYMGHIEVVKLLVSHGAEVTCKDKKSYTPLHAAASSGMISVVKYLLDLGVDMNEPNAYGNTPLHVACYNGQDVVVNELID 260          

HSP 4 Score: 47.7506 bits (112), Expect = 2.894e-6
Identity = 28/106 (26.42%), Postives = 53/106 (50.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEA----LDQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+DA  K+ +  +H   + GH E V+ L++      + D      IH + + GHI  +  L+     ++A    +D + +  +H+AC  GH   V+LL++
Sbjct:  672 LLNKGANVDAKDKWGRTALHRGAVTGHEECVDALLQHGAKCLLRDSRGRTPIHLSAACGHIGVLGALLQSATSVDANPAVVDNHGYTALHWACYNGHETCVELLLE 777          
BLAST of SMED30003476 vs. UniProt/SwissProt
Match: sp|Q5UPG5|YL093_MIMIV (Putative ankyrin repeat protein L93 OS=Acanthamoeba polyphaga mimivirus OX=212035 GN=MIMI_L93 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 5.042e-13
Identity = 35/111 (31.53%), Postives = 60/111 (54.05%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFAC----IAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+D + +I+A  K     +  AC        IE + +L+ +  +I   +G  W  +  AC   +IEA+KLL+  + DI A D++ W P+ +AC   +++ +KLL+D   DI
Sbjct:  307 LLDKNADINAKDKIGLTALMLACKYANTPNSIEIIKLLLDKGADINTGNGEGWTALMFACKYANIEAIKLLLDKNADINAKDKDGWTPLMFACVYANIETIKLLLDKRADI 417          
BLAST of SMED30003476 vs. UniProt/SwissProt
Match: sp|Q8N8A2|ANR44_HUMAN (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Homo sapiens OX=9606 GN=ANKRD44 PE=1 SV=3)

HSP 1 Score: 66.2402 bits (160), Expect = 8.040e-13
Identity = 33/107 (30.84%), Postives = 57/107 (53.27%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI H  +++A  K  Q P+H A     ++   ++I    ++ + D      +HHA   GH+E V LL+    +I A D+ D R +H+A   GH+ +V LLI++  ++
Sbjct:   93 LIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEV 199          

HSP 2 Score: 64.3142 bits (155), Expect = 4.159e-12
Identity = 31/102 (30.39%), Postives = 55/102 (53.92%), Query Frame = 1
Query:   16 CNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
             +++   +  +  +H A + GH+E VN+L+ +  NI   D  D R +H A   GH++ V LLI H  ++   D+  + P+H A   G + +VK L++   +I
Sbjct:  131 SSVNVSDRGGRTALHHAALNGHVEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQINVVKHLLNLGVEI 232          

HSP 3 Score: 55.0694 bits (131), Expect = 6.879e-9
Identity = 33/107 (30.84%), Postives = 53/107 (49.53%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NI+A  K D+R +H+A   GH++ V +LI     +   D   +  +H A S G I  VK L+    +I+ ++      +H AC  G   +V  LIDY  ++
Sbjct:  159 LLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQINVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANV 265          

HSP 4 Score: 51.2174 bits (121), Expect = 1.444e-7
Identity = 26/93 (27.96%), Postives = 44/93 (47.31%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGH 279
            + D+   +D      Q P+  A   GHI+ V++L+++  N++ +D      +H     GH E V++L+     I   D     P+HYA   GH
Sbjct:  656 IADNPEAVDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDTVDILGCTALHRGIMTGHEECVQMLLEQEVSILCKDSRGRTPLHYAAARGH 748          

HSP 5 Score: 48.9062 bits (115), Expect = 1.070e-6
Identity = 26/95 (27.37%), Postives = 46/95 (48.42%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            +++ L    + P+H A   G  E + +LI     +   D      +H A ++   EAV++LI H  D+ A D+N   P+H A     +K  +++I
Sbjct:   33 DVNTLDSEKRTPLHVAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVII 127          

HSP 6 Score: 46.595 bits (109), Expect = 7.479e-6
Identity = 26/107 (24.30%), Postives = 46/107 (42.99%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    +    G +   P+H A +  H +    L+   + I+  D F    +H A + G++E +KLL     D    D+    P+HYA    H   ++ L+    ++
Sbjct:  358 LITSGADTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGFEIDTPDKFGRTCLHAAAAGGNVECIKLLQSSGADFHKKDKCGRTPLHYAAANCHFHCIETLVTTGANV 464          
BLAST of SMED30003476 vs. UniProt/SwissProt
Match: sp|B2RXR6|ANR44_MOUSE (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Mus musculus OX=10090 GN=Ankrd44 PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.233e-12
Identity = 33/107 (30.84%), Postives = 57/107 (53.27%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI H  +++A  K  Q P+H A     ++   ++I    ++ + D      +HHA   GH+E V LL+    +I A D+ D R +H+A   GH+ +V LLI++  ++
Sbjct:   93 LIKHSADVNARDKNWQTPLHVAAANKAVKCAEVIIPLLSSVNVSDRGGRTALHHAALNGHMEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEV 199          

HSP 2 Score: 63.929 bits (154), Expect = 6.078e-12
Identity = 31/102 (30.39%), Postives = 55/102 (53.92%), Query Frame = 1
Query:   16 CNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
             +++   +  +  +H A + GH+E VN+L+ +  NI   D  D R +H A   GH++ V LLI H  ++   D+  + P+H A   G + +VK L++   +I
Sbjct:  131 SSVNVSDRGGRTALHHAALNGHMEMVNLLLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQISVVKHLLNLGVEI 232          

HSP 3 Score: 55.0694 bits (131), Expect = 6.553e-9
Identity = 33/107 (30.84%), Postives = 53/107 (49.53%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NI+A  K D+R +H+A   GH++ V +LI     +   D   +  +H A S G I  VK L+    +I+ ++      +H AC  G   +V  LIDY  ++
Sbjct:  159 LLAKGANINAFDKKDRRALHWAAYMGHLDVVALLINHGAEVTCKDKKGYTPLHAAASNGQISVVKHLLNLGVEIDEINVYGNTALHIACYNGQDAVVNELIDYGANV 265          

HSP 4 Score: 50.8322 bits (120), Expect = 2.588e-7
Identity = 25/86 (29.07%), Postives = 41/86 (47.67%), Query Frame = 1
Query:   22 IDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGH 279
            +D      Q P+  A   GHI+ V++L+++  N++ +D      +H     GH E V++L+     I   D     P+HYA   GH
Sbjct:  663 VDVKDAKGQTPLMLAVAYGHIDAVSLLLEKEANVDAVDIVGCTALHRGIMTGHEECVQMLLEQEASILCKDSRGRTPLHYAAARGH 748          

HSP 5 Score: 50.447 bits (119), Expect = 3.532e-7
Identity = 27/95 (28.42%), Postives = 47/95 (49.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            +++AL    + P+H A   G  E + +LI     +   D      +H A ++   EAV++LI H  D+ A D+N   P+H A     +K  +++I
Sbjct:   33 DVNALDSEKRTPLHVAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVQVLIKHSADVNARDKNWQTPLHVAAANKAVKCAEVII 127          

HSP 6 Score: 47.7506 bits (112), Expect = 2.469e-6
Identity = 27/107 (25.23%), Postives = 46/107 (42.99%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    +    G +   P+H A +  H +    L+   + I+  D F    +H A + G++E +KLL     D    D+    P+HYA    H   +K L+    ++
Sbjct:  358 LITSGADTAKCGIHSMFPLHLAALNAHSDCCRKLLSSGFEIDTPDTFGRTCLHAAAAGGNVECIKLLQSSGADFHKKDKCGRTPLHYAAANCHFHCIKALVTTGANV 464          
BLAST of SMED30003476 vs. TrEMBL
Match: A0A182GGY8 (ANK_REP_REGION domain-containing protein OS=Aedes albopictus OX=7160 GN=RP20_CCG009719 PE=4 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 7.820e-22
Identity = 48/107 (44.86%), Postives = 69/107 (64.49%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NI+AL + +  P+HFAC  GH E V ILI+E  NIE L+  +W  +H +C  GH E V++LI    +IEAL+Q++W P+H AC  GH ++V++LI    +I
Sbjct: 1418 LIRGKANIEALNQVNWTPLHFACHNGHKEVVEILIREKVNIEALNQDNWTPLHLSCRHGHKEVVEILIREKVNIEALNQDNWTPLHLACQDGHKEVVEILIREEANI 1524          

HSP 2 Score: 97.8265 bits (242), Expect = 2.277e-21
Identity = 47/107 (43.93%), Postives = 66/107 (61.68%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NIDAL +    P+H AC  GH E V ILI+   NIE L+  +W  +H AC  GH E V++LI    +IEAL+Q++W P+H +C  GH ++V++LI    +I
Sbjct: 1385 LIGEKANIDALTEKKSTPLHLACQNGHKEVVEILIRGKANIEALNQVNWTPLHFACHNGHKEVVEILIREKVNIEALNQDNWTPLHLSCRHGHKEVVEILIREKVNI 1491          

HSP 3 Score: 96.2857 bits (238), Expect = 8.701e-21
Identity = 46/107 (42.99%), Postives = 66/107 (61.68%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NI+AL +Y+  P+H A   GH + V ILI+E  NIE L   +W  +H AC  GH E V++LI    +IEAL Q++W P+H AC  GH ++V++L+    +I
Sbjct: 1517 LIREEANIEALNQYNYTPLHIASWNGHEKVVEILIREKANIEALGQENWTPLHLACRNGHKEVVEILIRGKANIEALTQDNWTPLHKACQNGHAEVVEILVKEKANI 1623          

HSP 4 Score: 95.5153 bits (236), Expect = 1.559e-20
Identity = 47/107 (43.93%), Postives = 65/107 (60.75%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NIDAL K    P+HFAC  G  E V ILI+E  NIE L+  +W  +H AC  GH E V++LI  + +I+AL ++ + P+H AC  GH + V++LI    +I
Sbjct: 1220 LIGGKANIDALAKNKSTPLHFACQNGRKEVVEILIREKANIEALNQHNWTPLHLACQNGHKEVVEILIKENANIDALTEHKYTPLHLACARGHKEAVQILIGEKANI 1326          

HSP 5 Score: 93.9745 bits (232), Expect = 5.786e-20
Identity = 46/107 (42.99%), Postives = 68/107 (63.55%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NI+AL +++  P+H AC  GH E V ILIKE  NI+ L    +  +H AC+ GH EAV++LI    +I+ALD+N + P+H AC  GH ++V++L+    +I
Sbjct: 1253 LIREKANIEALNQHNWTPLHLACQNGHKEVVEILIKENANIDALTEHKYTPLHLACARGHKEAVQILIGEKANIDALDENKFTPLHKACQNGHAEVVEILVKEKANI 1359          

HSP 6 Score: 92.8189 bits (229), Expect = 1.271e-19
Identity = 46/107 (42.99%), Postives = 63/107 (58.88%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NIDAL +    P+H AC  GH E V ILI E  NI+ L       +H AC  GH E V++L+    +IEAL+Q++W P+H AC  GH ++VK+LI    +I
Sbjct: 1616 LVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILVGEKANIEALNQDNWTPLHLACEKGHKEVVKILIGEKANI 1722          

HSP 7 Score: 91.6633 bits (226), Expect = 3.231e-19
Identity = 45/107 (42.06%), Postives = 62/107 (57.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NIDAL +    P+H AC  GH E V IL+ E  NIE L+  +W  +H AC  GH E VK+LI    +I+AL Q    P+H AC  GH ++V++ +  + +I
Sbjct: 1649 LIGEKANIDALTEKKSTPLHLACQNGHKEVVEILVGEKANIEALNQDNWTPLHLACEKGHKEVVKILIGEKANIDALTQKKNTPLHLACQRGHKEVVEIFLRKNANI 1755          

HSP 8 Score: 91.6633 bits (226), Expect = 3.359e-19
Identity = 44/107 (41.12%), Postives = 64/107 (59.81%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NI+ALG+ +  P+H AC  GH E V ILI+   NIE L   +W  +H AC  GH E V++L+    +I+AL +  + P+H AC  GH ++V++LI    +I
Sbjct: 1550 LIREKANIEALGQENWTPLHLACRNGHKEVVEILIRGKANIEALTQDNWTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANI 1656          

HSP 9 Score: 90.8929 bits (224), Expect = 6.195e-19
Identity = 46/107 (42.99%), Postives = 66/107 (61.68%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NI+AL + +  P+H +C  GH E V ILI+E  NIE L+  +W  +H AC  GH E V++LI    +IEAL+Q ++ P+H A   GH K+V++LI    +I
Sbjct: 1451 LIREKVNIEALNQDNWTPLHLSCRHGHKEVVEILIREKVNIEALNQDNWTPLHLACQDGHKEVVEILIREEANIEALNQYNYTPLHIASWNGHEKVVEILIREKANI 1557          

HSP 10 Score: 90.1225 bits (222), Expect = 9.877e-19
Identity = 46/107 (42.99%), Postives = 63/107 (58.88%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NIDAL +    P+H AC  GH E V ILI E  NI+ L       +H AC  GH E V++LI    +IEAL+Q +W P+H+AC  GH ++V++LI    +I
Sbjct: 1352 LVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILIRGKANIEALNQVNWTPLHFACHNGHKEVVEILIREKVNI 1458          

HSP 11 Score: 90.1225 bits (222), Expect = 1.200e-18
Identity = 44/107 (41.12%), Postives = 64/107 (59.81%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI  + NIDAL ++   P+H AC  GH E V ILI E  NI+ LD   +  +H AC  GH E V++L+    +I+AL +  + P+H AC  GH ++V++LI    +I
Sbjct: 1286 LIKENANIDALTEHKYTPLHLACARGHKEAVQILIGEKANIDALDENKFTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANI 1392          

HSP 12 Score: 89.7373 bits (221), Expect = 1.485e-18
Identity = 45/107 (42.06%), Postives = 66/107 (61.68%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NI+AL + +  P+H AC  GH E V ILI+E  NIE L+ +++  +H A   GH + V++LI    +IEAL Q +W P+H AC  GH ++V++LI    +I
Sbjct: 1484 LIREKVNIEALNQDNWTPLHLACQDGHKEVVEILIREEANIEALNQYNYTPLHIASWNGHEKVVEILIREKANIEALGQENWTPLHLACRNGHKEVVEILIRGKANI 1590          

HSP 13 Score: 84.3445 bits (207), Expect = 1.133e-16
Identity = 42/107 (39.25%), Postives = 65/107 (60.75%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+D+  N++A    +  P+H+ACI+G  E V ILI    NI+ L       +H AC  G  E V++LI    +IEAL+Q++W P+H AC  GH ++V++LI  + +I
Sbjct: 1187 LLDNGANVNAADTNEYTPLHWACISGVKEVVEILIGGKANIDALAKNKSTPLHFACQNGRKEVVEILIREKANIEALNQHNWTPLHLACQNGHKEVVEILIKENANI 1293          

HSP 14 Score: 82.8037 bits (203), Expect = 4.593e-16
Identity = 44/107 (41.12%), Postives = 61/107 (57.01%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NIDAL +    P+H AC  GH E V IL+KE  NI+ L    +  +H AC  GH E V++LI    +I+AL +    P+H AC  GH ++V++LI    +I
Sbjct: 1319 LIGEKANIDALDENKFTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILIRGKANI 1425          

HSP 15 Score: 80.8777 bits (198), Expect = 1.809e-15
Identity = 42/107 (39.25%), Postives = 62/107 (57.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NI+AL + +  P+H AC  GH E V IL+KE  NI+ L    +  +H AC  GH E V++LI    +I+AL +    P+H AC  GH ++V++L+    +I
Sbjct: 1583 LIRGKANIEALTQDNWTPLHKACQNGHAEVVEILVKEKANIDALAEKKFTPLHLACQNGHKEVVEILIGEKANIDALTEKKSTPLHLACQNGHKEVVEILVGEKANI 1689          

HSP 16 Score: 69.707 bits (169), Expect = 1.652e-11
Identity = 36/100 (36.00%), Postives = 54/100 (54.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLL 300
            L+    NI+AL + +  P+H AC  GH E V ILI E  NI+ L       +H AC  GH E V++ +  + +I ALD     P++ A   GH ++ K++
Sbjct: 1682 LVGEKANIEALNQDNWTPLHLACEKGHKEVVKILIGEKANIDALTQKKNTPLHLACQRGHKEVVEIFLRKNANIAALDDEKCTPLNLASQNGHTEVEKIM 1781          

HSP 17 Score: 61.6178 bits (148), Expect = 1.195e-8
Identity = 32/107 (29.91%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI++  N++A        +H +  +GH   V +L+    N+   D  ++  +H AC +G  E V++LI    +I+AL +N   P+H+AC  G  ++V++LI    +I
Sbjct: 1154 LINNGANVNAPSVAGYTALHISASSGHRPIVELLLDNGANVNAADTNEYTPLHWACISGVKEVVEILIGGKANIDALAKNKSTPLHFACQNGRKEVVEILIREKANI 1260          
BLAST of SMED30003476 vs. TrEMBL
Match: A0A1X7T6P4 (Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 7.175e-20
Identity = 47/110 (42.73%), Postives = 68/110 (61.82%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKE-YYNIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY-HCDI 321
            +ID HC+++A G Y   P+H+AC  GH E V IL      +IE  D  + R +H AC + ++E V+ L+I   CD+ A   ND+ P+HYAC  GH +IVK+L D+  C+I
Sbjct:  601 VIDKHCDVNAKGDYGHTPLHYACREGHFEAVKILTNHPQCDIEAKDKINNRPLHEACKSENVETVRHLVIDKCCDVNAKGMNDYTPLHYACAKGHFEIVKILTDHPQCNI 710          

HSP 2 Score: 85.1149 bits (209), Expect = 7.185e-17
Identity = 46/110 (41.82%), Postives = 65/110 (59.09%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKE-YYNIEILDGFDWRLIHHACSAGHIEAV-KLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY-HCDI 321
            LID HC+I+A G+    P+H+AC  GH E V IL      NIE+ D  + R +H AC  G+++ +  L+I  HCD+ A       P+HYAC  GH + VK+L ++  CDI
Sbjct:  533 LIDKHCDINAKGRNGHTPLHYACEKGHFEIVKILTNHPQCNIEVKDKNNDRPLHKACVTGNLDILCHLVIDKHCDVNAKGDYGHTPLHYACREGHFEAVKILTNHPQCDI 642          

HSP 3 Score: 81.6481 bits (200), Expect = 1.082e-15
Identity = 43/102 (42.16%), Postives = 62/102 (60.78%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKE-YYNIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLL 300
            +ID  C+++A G  D  P+H+AC  GH E V IL      NIE  +  + R +H AC++G+ + V+ L+I  HCD+ A   N + P+HYAC  GH K VK+L
Sbjct:  669 VIDKCCDVNAKGMNDYTPLHYACAKGHFEIVKILTDHPQCNIEAKEYSNDRPLHEACTSGNADIVRHLVIDKHCDVNAKGGNGYTPLHYACENGHFKTVKIL 770          

HSP 4 Score: 66.2402 bits (160), Expect = 2.829e-10
Identity = 43/125 (34.40%), Postives = 67/125 (53.60%), Query Frame = 1
Query:   13 HCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYN------------------IEILDGF--DWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY-HCDI 321
             CN +A  K   +P+H+A   GH E ++IL K+                     +E+LD    D R + +AC +G+++ ++ LLI  HCDI A  +N   P+HYAC  GH +IVK+L ++  C+I
Sbjct:  450 QCNNEAKDKEGNQPLHYAACQGHKEIISILEKKVSMDGLSKCMKSAKQLSEPDIMELLDDCFEDRRSLINACESGNVDILRHLLIDKHCDINAKGRNGHTPLHYACEKGHFEIVKILTNHPQCNI 574          

HSP 5 Score: 60.077 bits (144), Expect = 4.235e-8
Identity = 35/104 (33.65%), Postives = 60/104 (57.69%), Query Frame = 1
Query:    1 LIDH-HCNIDALGKYDQRPIHFACIAGHIETV-NILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYH-HCD-IEALDQNDWRPIHYACCAGHMKIVKLL 300
            L DH  CNI+A    + RP+H AC +G+ + V +++I ++ ++    G  +  +H+AC  GH + VK+L +H  C  IEA + ++ RPI  A    H+ ++  L
Sbjct:  702 LTDHPQCNIEAKEYSNDRPLHEACTSGNADIVRHLVIDKHCDVNAKGGNGYTPLHYACENGHFKTVKILTHHPQCSTIEAKNNDNERPIELAFGVSHVNLIHYL 805          
BLAST of SMED30003476 vs. TrEMBL
Match: A0A1X7T5X6 (Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 3.909e-19
Identity = 45/105 (42.86%), Postives = 68/105 (64.76%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            +ID HCN++A G+    P+H+AC  GH E V IL      NIE+   F+ R +H AC +G+++ V+ L+I  HCD+ A+  N + P+HYAC  GH +IVK+L ++
Sbjct:   98 VIDKHCNVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNIEVEGSFNDRPLHKACESGNVDIVRHLVIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNH 202          

HSP 2 Score: 88.9669 bits (219), Expect = 2.979e-18
Identity = 45/105 (42.86%), Postives = 68/105 (64.76%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            +ID HC+++A GK    P+H+AC  GH E V IL      NIE  D ++ R +H AC +G+++ V+ L+I  HCD+ A+  N + P+HYAC  GH +IVK+L ++
Sbjct:  234 VIDKHCDVNAKGKDGYTPLHYACEKGHFEIVKILTNHPQCNIEAEDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNH 338          

HSP 3 Score: 88.5817 bits (218), Expect = 3.911e-18
Identity = 44/105 (41.90%), Postives = 67/105 (63.81%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            +ID HC+++A  +    P+H+AC  GH ETV IL      NIE  D ++ R +H AC +G+++ V+ L+I  HCD+ A  +N + P+HYAC  GH +IVK L ++
Sbjct:  710 VIDKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNIEAEDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAKGRNGYTPLHYACEKGHFEIVKFLTNH 814          

HSP 4 Score: 87.4261 bits (215), Expect = 9.108e-18
Identity = 45/110 (40.91%), Postives = 71/110 (64.55%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY-HCDI 321
            +I+ HC+++A  +    P+H+AC  GH ETV IL      NIE  D ++ R +H AC +G+++ V+ L+I  HCD+ A  +N + P+HYAC  GH +IVK+L ++  C+I
Sbjct:  370 VINKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNIEAEDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNI 479          

HSP 5 Score: 86.6557 bits (213), Expect = 1.781e-17
Identity = 43/105 (40.95%), Postives = 68/105 (64.76%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            +I+ HC+++A  +    P+H+AC  GH ETV IL      NIE  D ++ R +H AC +G+++ V+ L+I  HCD+ A+  N + P+HYAC  GH +IVK+L ++
Sbjct:  574 VINKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNIEAKDNWNNRPLHKACESGNVDIVRHLVIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNH 678          

HSP 6 Score: 86.2705 bits (212), Expect = 2.431e-17
Identity = 44/110 (40.00%), Postives = 69/110 (62.73%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY-HCDI 321
            +ID HC+++A G+    P+H+AC  GH E V IL      N+E    F+ R +H AC +G+++ V+ L+I  HCD+ A D+  + P+HYAC  GH + VK+L ++  C+I
Sbjct:  506 VIDKHCDVNAKGRIGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGSFNDRPLHKACESGNVDIVRHLVINKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNI 615          

HSP 7 Score: 85.1149 bits (209), Expect = 7.149e-17
Identity = 44/110 (40.00%), Postives = 69/110 (62.73%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY-HCDI 321
            +ID HC+++A+G     P+H+AC  GH E V IL      N+E    F+ R +H AC +G+++ V+ L+I  HCD+ A D+  + P+HYAC  GH + VK+L ++  C+I
Sbjct:  302 VIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGSFNDRPLHKACESGNVDIVRHLVINKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNI 411          

HSP 8 Score: 84.3445 bits (207), Expect = 1.151e-16
Identity = 44/110 (40.00%), Postives = 69/110 (62.73%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY-HCDI 321
            +ID HC+++A+G     P+H+AC  GH E V IL      N+E    F+ R +H AC +G+++ V+ L+I  HCD+ A D+  + P+HYAC  GH + VK+L ++  C+I
Sbjct:  642 VIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGSFNDRPLHKACESGNVDIVRHLVIDKHCDVNAKDRIGYTPLHYACEKGHFETVKILTNHPQCNI 751          

HSP 9 Score: 83.1889 bits (204), Expect = 2.681e-16
Identity = 44/110 (40.00%), Postives = 69/110 (62.73%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY-HCDI 321
            +ID HC+++A+G     P+H+AC  GH E V IL      N+E    F+ R +H AC +G+++ V  L+I  HCD+ A  ++ + P+HYAC  GH +IVK+L ++  C+I
Sbjct:  166 VIDKHCDVNAIGWNGYTPLHYACEKGHFEIVKILTNHSQCNLEAEGNFNDRPLHKACESGNVDIVHHLVIDKHCDVNAKGKDGYTPLHYACEKGHFEIVKILTNHPQCNI 275          

HSP 10 Score: 82.4185 bits (202), Expect = 5.243e-16
Identity = 43/105 (40.95%), Postives = 66/105 (62.86%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            +ID HC+++A G+    P+H+AC  GH E V IL      NIE  D    R +H+AC +G+++ V+ L+I  HCD+ A  +  + P+HYAC  GH +IVK+L ++
Sbjct:  438 VIDKHCDVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNIEAEDYSKDRPLHNACESGNVDIVRHLVIDKHCDVNAKGRIGYTPLHYACEKGHFEIVKILTNH 542          

HSP 11 Score: 82.0333 bits (201), Expect = 8.691e-16
Identity = 44/110 (40.00%), Postives = 69/110 (62.73%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAV-KLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY-HCDI 321
            +ID HC+++A G+    P+H+AC  G  E V IL      NIE+ D  + R +H AC +G+++ V  L+I  HC++ A  +N + P+HYAC  GH +IVK+L ++  C+I
Sbjct:   30 VIDKHCDVNAKGRNGYTPLHYACEEGCFEIVRILTDHPQCNIEVEDNSNDRPLHKACESGNVDIVCHLVIDKHCNVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNI 139          

HSP 12 Score: 78.1814 bits (191), Expect = 1.735e-14
Identity = 44/110 (40.00%), Postives = 66/110 (60.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY-HCDI 321
            +ID H +++A G+    P+H+AC  GH E V IL      N E  D +D R +H AC  G ++ V+ L+I  H D+ A  +N + P+HYAC  GH +IVK+L ++  C+I
Sbjct:  846 VIDKHRDVNAKGRDGYTPLHYACEKGHFEIVKILTNHPQCNTEAEDVYDDRSLHKACEFGRLDIVRYLVIDKHRDVNAKGRNGYTPLHYACEKGHFEIVKILTNHPQCNI 955          

HSP 13 Score: 74.7146 bits (182), Expect = 3.269e-13
Identity = 39/105 (37.14%), Postives = 63/105 (60.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYY-NIEILDGFDWRLIHHACSAGHIEAVK-LLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            +ID HC+++A G+    P+H+AC  GH E V  L      N +  D  D R +H AC + +++ V+ L+I  H D+ A  ++ + P+HYAC  GH +IVK+L ++
Sbjct:  778 VIDKHCDVNAKGRNGYTPLHYACEKGHFEIVKFLTNHPQCNTKAEDVCDDRPLHKACKSRNVDIVRYLVIDKHRDVNAKGRDGYTPLHYACEKGHFEIVKILTNH 882          
BLAST of SMED30003476 vs. TrEMBL
Match: A0A182H2I6 (Uncharacterized protein OS=Aedes albopictus OX=7160 GN=RP20_CCG020406 PE=4 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 6.163e-19
Identity = 46/107 (42.99%), Postives = 62/107 (57.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NIDAL   +  P+H AC+ GH E V ILIKE  N++ LD      +H AC  GH E V++LI    +I AL   ++ P+H AC  GH ++VK+LI    +I
Sbjct: 1121 LIRRKANIDALALENSTPLHLACLNGHKEVVEILIKEKANVDTLDERKLTPLHFACHKGHKEVVEVLIREEANINALAFENFTPLHLACLNGHKEVVKVLIREKANI 1227          

HSP 2 Score: 82.4185 bits (202), Expect = 5.660e-16
Identity = 43/107 (40.19%), Postives = 60/107 (56.07%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    N+D L +    P+HFAC  GH E V +LI+E  NI  L   ++  +H AC  GH E VK+LI    +IEAL   +  P+H AC  GH ++ ++LI    +I
Sbjct: 1154 LIKEKANVDTLDERKLTPLHFACHKGHKEVVEVLIREEANINALAFENFTPLHLACLNGHKEVVKVLIREKANIEALALENCTPLHLACLNGHKEVAEILIREKSNI 1260          

HSP 3 Score: 81.2629 bits (199), Expect = 1.617e-15
Identity = 40/107 (37.38%), Postives = 62/107 (57.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NI+AL   +  P+H AC+ GH E V IL++E  NI+ LD      +H AC  GH E V++LI    +I+A+   ++ P+H AC  G  ++V++LI    +I
Sbjct: 1319 LVREKININALALENCTPLHLACLNGHKEVVEILVREKANIDALDEKKSTPLHLACQNGHKEVVEILIREKANIDAMALENYTPLHLACLNGQKEVVEVLIREKANI 1425          

HSP 4 Score: 79.337 bits (194), Expect = 7.367e-15
Identity = 42/107 (39.25%), Postives = 61/107 (57.01%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NIDAL   +  P+H AC  GHIE V ILI+E  N++ L   +   +H AC  GH E  ++LI    ++ ALD+    P+H AC  GH ++V++LI    +I
Sbjct: 1550 LVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNVDALAFENCTPLHLACLNGHKEVAEVLIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKANI 1656          

HSP 5 Score: 79.337 bits (194), Expect = 7.810e-15
Identity = 42/107 (39.25%), Postives = 62/107 (57.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NI+AL   +  P+H AC+ GH E V +LI+E  NIE L   +   +H AC  GH E  ++LI    +I+AL+  +  P+H AC  GH ++V++LI    +I
Sbjct: 1187 LIREEANINALAFENFTPLHLACLNGHKEVVKVLIREKANIEALALENCTPLHLACLNGHKEVAEILIREKSNIDALELKNCTPLHLACLNGHNEVVEILIRGKANI 1293          

HSP 6 Score: 78.9518 bits (193), Expect = 1.056e-14
Identity = 39/107 (36.45%), Postives = 60/107 (56.07%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    N+DAL   +  P+H AC+ GH E   +LI++  N+  LD      +H AC  GH E V++LI    +I+A D  +  P+H AC  GH ++V++L+    +I
Sbjct: 1583 LIREKSNVDALAFENCTPLHLACLNGHKEVAEVLIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKANIDATDFRNCTPLHLACFNGHKEVVEILVREKANI 1689          

HSP 7 Score: 78.5666 bits (192), Expect = 1.119e-14
Identity = 42/107 (39.25%), Postives = 60/107 (56.07%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NIDAL   +  P+H AC  GHIE V ILI+E  N++ L   +   +H AC  GH E  ++LI    ++ ALD+    P+H AC  GH ++V++LI     I
Sbjct: 1682 LVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNVDALAFENCTPLHLACLNGHKEVAEVLIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKASI 1788          

HSP 8 Score: 78.5666 bits (192), Expect = 1.119e-14
Identity = 42/107 (39.25%), Postives = 60/107 (56.07%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NIDAL   +  P+H AC  GHIE V ILI+E  N++ L   +   +H AC  GH E  ++LI    ++ ALD+    P+H AC  GH ++V++LI     I
Sbjct: 1946 LVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNVDALAFENCTPLHLACLNGHKEVAEVLIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKASI 2052          

HSP 9 Score: 78.1814 bits (191), Expect = 1.542e-14
Identity = 40/107 (37.38%), Postives = 60/107 (56.07%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NIDA+   +  P+H AC  G  E V +L++E  NI  L   +   +H AC  GH E V++L+    +I+ALD+    P+H AC  GH ++V++LI    +I
Sbjct: 1286 LIRGKANIDAMALENYTPLHLACQNGQKEVVKVLVREKININALALENCTPLHLACLNGHKEVVEILVREKANIDALDEKKSTPLHLACQNGHKEVVEILIREKANI 1392          

HSP 10 Score: 77.7962 bits (190), Expect = 2.607e-14
Identity = 38/107 (35.51%), Postives = 59/107 (55.14%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    N+DAL   +  P+H AC+ GH E   +LI++  N+  LD      +H AC  GH E V++LI     I+A+   ++ P+H AC  G  ++V++LI    +I
Sbjct: 1715 LIREKSNVDALAFENCTPLHLACLNGHKEVAEVLIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKASIDAMALENYTPLHLACLNGQKEVVEVLIREKANI 1821          

HSP 11 Score: 77.7962 bits (190), Expect = 2.607e-14
Identity = 38/107 (35.51%), Postives = 59/107 (55.14%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    N+DAL   +  P+H AC+ GH E   +LI++  N+  LD      +H AC  GH E V++LI     I+A+   ++ P+H AC  G  ++V++LI    +I
Sbjct: 1979 LIREKSNVDALAFENCTPLHLACLNGHKEVAEVLIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKASIDAMALENYTPLHLACLNGQKEVVEVLIREKANI 2085          

HSP 12 Score: 77.411 bits (189), Expect = 3.558e-14
Identity = 40/107 (37.38%), Postives = 62/107 (57.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            +I  + NI AL   +  P+H AC+ GH E V ILI+   NI+ L   +   +H AC  GH E V++LI    +++ LD+    P+H+AC  GH ++V++LI    +I
Sbjct: 1088 IIKGNVNIGALTHENCTPLHLACLNGHKEIVEILIRRKANIDALALENSTPLHLACLNGHKEVVEILIKEKANVDTLDERKLTPLHFACHKGHKEVVEVLIREEANI 1194          

HSP 13 Score: 77.411 bits (189), Expect = 3.593e-14
Identity = 40/101 (39.60%), Postives = 59/101 (58.42%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            LI    NIDA    +  P+H AC  GH E V IL++E  NI+ L+  +   +H AC  GHIE V++LI    +++AL   +  P+H AC  GH ++ ++LI
Sbjct: 1649 LIRGKANIDATDFRNCTPLHLACFNGHKEVVEILVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNVDALAFENCTPLHLACLNGHKEVAEVLI 1749          

HSP 14 Score: 77.411 bits (189), Expect = 3.593e-14
Identity = 40/101 (39.60%), Postives = 59/101 (58.42%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            LI    NIDA    +  P+H AC  GH E V IL++E  NI+ L+  +   +H AC  GHIE V++LI    +++AL   +  P+H AC  GH ++ ++LI
Sbjct: 1913 LIRGKANIDATDFRNCTPLHLACFNGHKEVVEILVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNVDALAFENCTPLHLACLNGHKEVAEVLI 2013          

HSP 15 Score: 77.411 bits (189), Expect = 3.664e-14
Identity = 42/107 (39.25%), Postives = 59/107 (55.14%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NIDAL   +  PIH A + GH E V IL +E  NI+  D  +   +H AC  GH E  ++LI    ++ ALD+    P+H AC  GH ++V++LI    +I
Sbjct: 1814 LIREKANIDALALENCTPIHLASLNGHKEVVEILAREKANIDATDFRNCTPLHLACLNGHKEVAEVLIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKANI 1920          

HSP 16 Score: 76.6406 bits (187), Expect = 5.199e-14
Identity = 40/107 (37.38%), Postives = 62/107 (57.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NI+AL   +  P+H AC+ GH E   ILI+E  NI+ L+  +   +H AC  GH E V++LI    +I+A+   ++ P+H AC  G  ++VK+L+    +I
Sbjct: 1220 LIREKANIEALALENCTPLHLACLNGHKEVAEILIREKSNIDALELKNCTPLHLACLNGHNEVVEILIRGKANIDAMALENYTPLHLACQNGQKEVVKVLVREKINI 1326          

HSP 17 Score: 76.2554 bits (186), Expect = 9.592e-14
Identity = 40/107 (37.38%), Postives = 60/107 (56.07%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NIDAL   +  P+H AC+ GH E V ILI+   NI+ +   ++  +H AC  G  E VK+L+    +I AL   +  P+H AC  GH ++V++L+    +I
Sbjct: 1253 LIREKSNIDALELKNCTPLHLACLNGHNEVVEILIRGKANIDAMALENYTPLHLACQNGQKEVVKVLVREKININALALENCTPLHLACLNGHKEVVEILVREKANI 1359          

HSP 18 Score: 75.8702 bits (185), Expect = 1.177e-13
Identity = 39/107 (36.45%), Postives = 62/107 (57.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    N++AL +    P+H AC  GH E V ILI+   NI+  D  +   +H AC  GH E V++L+    +I+AL+  +  P+H AC  GH+++V++LI    ++
Sbjct: 1616 LIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKANIDATDFRNCTPLHLACFNGHKEVVEILVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNV 1722          

HSP 19 Score: 75.8702 bits (185), Expect = 1.177e-13
Identity = 39/107 (36.45%), Postives = 62/107 (57.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    N++AL +    P+H AC  GH E V ILI+   NI+  D  +   +H AC  GH E V++L+    +I+AL+  +  P+H AC  GH+++V++LI    ++
Sbjct: 1880 LIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKANIDATDFRNCTPLHLACFNGHKEVVEILVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNV 1986          

HSP 20 Score: 75.485 bits (184), Expect = 1.335e-13
Identity = 42/107 (39.25%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    NIDAL   +  PIH A + GH E V IL  E  NI+  D  +   +H AC  GH E  ++LI    ++ ALD+    P+H AC  GH ++V++LI    +I
Sbjct: 1418 LIREKANIDALALENCTPIHLASLNGHKEVVEILAGEKANIDATDFRNCTPLHLACLNGHKEVAEVLIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKANI 1524          

HSP 21 Score: 75.485 bits (184), Expect = 1.736e-13
Identity = 40/107 (37.38%), Postives = 59/107 (55.14%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NIDAL +    P+H AC  GH E V ILI+E  NI+ +   ++  +H AC  G  E V++LI    +I+AL   +  PIH A   GH ++V++L     +I
Sbjct: 1352 LVREKANIDALDEKKSTPLHLACQNGHKEVVEILIREKANIDAMALENYTPLHLACLNGQKEVVEVLIREKANIDALALENCTPIHLASLNGHKEVVEILAGEKANI 1458          

HSP 22 Score: 75.0998 bits (183), Expect = 2.346e-13
Identity = 38/107 (35.51%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L     NIDA    +  P+H AC+ GH E   +LI++  N+  LD      +H AC  GH E V++LI    +I+A D  +  P+H AC  GH ++V++L+    +I
Sbjct: 1847 LAREKANIDATDFRNCTPLHLACLNGHKEVAEVLIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKANIDATDFRNCTPLHLACFNGHKEVVEILVREKANI 1953          

HSP 23 Score: 74.7146 bits (182), Expect = 3.080e-13
Identity = 39/101 (38.61%), Postives = 58/101 (57.43%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            LI    NI A    +  P+H AC  GH E V IL++E  NI+ L+  +   +H AC  GHIE V++LI    +++AL   +  P+H AC  GH ++ ++LI
Sbjct: 1517 LIRGKANIGATDFRNCTPLHLACFNGHKEVVEILVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNVDALAFENCTPLHLACLNGHKEVAEVLI 1617          

HSP 24 Score: 74.7146 bits (182), Expect = 3.172e-13
Identity = 39/107 (36.45%), Postives = 61/107 (57.01%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    N++AL +    P+H AC  GH E V ILI+   NI   D  +   +H AC  GH E V++L+    +I+AL+  +  P+H AC  GH+++V++LI    ++
Sbjct: 1484 LIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKANIGATDFRNCTPLHLACFNGHKEVVEILVREKANIDALEFRNCTPLHLACQNGHIEVVEILIREKSNV 1590          

HSP 25 Score: 73.559 bits (179), Expect = 6.264e-13
Identity = 38/107 (35.51%), Postives = 57/107 (53.27%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L     NIDA    +  P+H AC+ GH E   +LI++  N+  LD      +H AC  GH E V++LI    +I A D  +  P+H AC  GH ++V++L+    +I
Sbjct: 1451 LAGEKANIDATDFRNCTPLHLACLNGHKEVAEVLIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKANIGATDFRNCTPLHLACFNGHKEVVEILVREKANI 1557          

HSP 26 Score: 70.8626 bits (172), Expect = 6.036e-12
Identity = 38/101 (37.62%), Postives = 56/101 (55.45%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            LI    NIDA+   +  P+H AC+ G  E V +LI+E  NI+ L   +   IH A   GH E V++L     +I+A D  +  P+H AC  GH ++ ++LI
Sbjct: 1385 LIREKANIDAMALENYTPLHLACLNGQKEVVEVLIREKANIDALALENCTPIHLASLNGHKEVVEILAGEKANIDATDFRNCTPLHLACLNGHKEVAEVLI 1485          

HSP 27 Score: 68.9366 bits (167), Expect = 3.246e-11
Identity = 37/101 (36.63%), Postives = 56/101 (55.45%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            LI    +IDA+   +  P+H AC+ G  E V +LI+E  NI+ L   +   IH A   GH E V++L     +I+A D  +  P+H AC  GH ++ ++LI
Sbjct: 1781 LIRGKASIDAMALENYTPLHLACLNGQKEVVEVLIREKANIDALALENCTPIHLASLNGHKEVVEILAREKANIDATDFRNCTPLHLACLNGHKEVAEVLI 1881          

HSP 28 Score: 67.0106 bits (162), Expect = 1.410e-10
Identity = 37/107 (34.58%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    N++AL +    P+H AC  GH E V ILI+   +I+ +   ++  +H AC  G  E V++LI    +I+AL   +  PIH A   GH ++V++L     +I
Sbjct: 1748 LIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKASIDAMALENYTPLHLACLNGQKEVVEVLIREKANIDALALENCTPIHLASLNGHKEVVEILAREKANI 1854          

HSP 29 Score: 67.0106 bits (162), Expect = 1.410e-10
Identity = 37/107 (34.58%), Postives = 58/107 (54.21%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI    N++AL +    P+H AC  GH E V ILI+   +I+ +   ++  +H AC  G  E V++LI    +I+AL   +  PIH A   GH ++V++L     +I
Sbjct: 2012 LIRKVANVNALDEKKATPLHLACQNGHKEVVEILIRGKASIDAMALENYTPLHLACLNGQKEVVEVLIREKANIDALALENCTPIHLASLNGHKEVVEILAREKANI 2118          

HSP 30 Score: 55.8398 bits (133), Expect = 1.317e-6
Identity = 31/86 (36.05%), Postives = 46/86 (53.49%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIH 258
            LI    +IDA+   +  P+H AC+ G  E V +LI+E  NI+ L   +   IH A   GH E V++L     +I+A D  +  P+H
Sbjct: 2045 LIRGKASIDAMALENYTPLHLACLNGQKEVVEVLIREKANIDALALENCTPIHLASLNGHKEVVEILAREKANIDATDFRNCTPLH 2130          
BLAST of SMED30003476 vs. TrEMBL
Match: A0A1B8FHL1 (ANK_REP_REGION domain-containing protein OS=Pseudogymnoascus sp. 03VT05 OX=1622148 GN=VE02_08771 PE=4 SV=1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.484e-18
Identity = 36/107 (33.64%), Postives = 65/107 (60.75%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+D+  ++ A+      P+H+AC  G+++   +LI+   ++  ++G  W  +H+ACS G+++  KLLI    D+ A++   W P+HYAC  G++ + KLLID   D+
Sbjct:   33 LLDYGADVSAIEGRQWTPLHYACSNGNLDVAKLLIERGADVSAIEGRQWTPLHYACSNGNLDVAKLLIDRGADVSAINGRPWTPLHYACSNGNLDVAKLLIDRGADL 139          

HSP 2 Score: 78.1814 bits (191), Expect = 1.309e-14
Identity = 31/90 (34.44%), Postives = 54/90 (60.00%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            P+H A + G++    +L+    ++  ++G  W  +H+ACS G+++  KLLI    D+ A++   W P+HYAC  G++ + KLLID   D+
Sbjct:   17 PLHTAAMFGYLTVAELLLDYGADVSAIEGRQWTPLHYACSNGNLDVAKLLIERGADVSAIEGRQWTPLHYACSNGNLDVAKLLIDRGADV 106          
BLAST of SMED30003476 vs. Ensembl Cavefish
Match: ANKRD52 (ankyrin repeat domain 52 [Source:HGNC Symbol;Acc:HGNC:26614])

HSP 1 Score: 60.8474 bits (146), Expect = 7.612e-12
Identity = 30/89 (33.71%), Postives = 49/89 (55.06%), Query Frame = 1
Query:   55 IHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            +H A  +GH E V +L+ +  N+   D  D + IH A   GH+E VK+L+  + D+   D+  + P+H A   GH+ +VK L+    +I
Sbjct:  132 LHHAAYSGHTEMVTLLLNKGANLSASDKKDRQAIHWAAYLGHLEIVKVLVSRNADVACRDKRGYTPLHVAAINGHIDVVKYLLRLGAEI 220          

HSP 2 Score: 55.0694 bits (131), Expect = 7.429e-10
Identity = 30/107 (28.04%), Postives = 53/107 (49.53%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+     + A  +  Q P+H A     +     L+    ++ + D      +HHA  +GH E V LL+    ++ A D+ D + IH+A   GH++IVK+L+  + D+
Sbjct:   81 LLRQGAEVSARDRLWQTPLHVAAANRAMRCAEALVPLLSSLNVADRNGRTALHHAAYSGHTEMVTLLLNKGANLSASDKKDRQAIHWAAYLGHLEIVKVLVSRNADV 187          

HSP 3 Score: 48.9062 bits (115), Expect = 1.390e-7
Identity = 28/102 (27.45%), Postives = 50/102 (49.02%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L++   N+ A  K D++ IH+A   GH+E V +L+    ++   D   +  +H A   GHI+ VK L+    +I+  +      +H AC  G   +   L++
Sbjct:  147 LLNKGANLSASDKKDRQAIHWAAYLGHLEIVKVLVSRNADVACRDKRGYTPLHVAAINGHIDVVKYLLRLGAEIDEPNAFGNTSLHMACYNGQEAVANELVN 248          
BLAST of SMED30003476 vs. Ensembl Cavefish
Match: ankrd44 (ankyrin repeat domain 44 [Source:ZFIN;Acc:ZDB-GENE-070912-605])

HSP 1 Score: 60.8474 bits (146), Expect = 8.069e-12
Identity = 33/107 (30.84%), Postives = 54/107 (50.47%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI H  +++A  K  Q P+H A     +    ++I    ++ + D      +HHA   GH E V LL+    +I A D+ D R +H+A   GH+ +V LL+D   ++
Sbjct:   93 LIRHSADVNARDKNWQTPLHVAAANKALRCAEVIIPLLSSVNVSDRGGRTALHHAALNGHTEMVSLLLSKGANINAFDKKDGRALHWAAYMGHLDVVCLLVDQGAEV 199          

HSP 2 Score: 55.8398 bits (133), Expect = 4.613e-10
Identity = 28/89 (31.46%), Postives = 48/89 (53.93%), Query Frame = 1
Query:   55 IHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            +H A + GH E V++L+ +  NI   D  D R +H A   GH++ V LL+    ++   D+  + P+H A   G + +VK L++   +I
Sbjct:  144 LHHAALNGHTEMVSLLLSKGANINAFDKKDGRALHWAAYMGHLDVVCLLVDQGAEVSCKDKRGYTPLHAAASNGQINVVKHLLNLSVEI 232          

HSP 3 Score: 53.1434 bits (126), Expect = 3.556e-9
Identity = 32/107 (29.91%), Postives = 52/107 (48.60%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NI+A  K D R +H+A   GH++ V +L+ +   +   D   +  +H A S G I  VK L+    +I+  +      +H AC  G   +V  LIDY  ++
Sbjct:  159 LLSKGANINAFDKKDGRALHWAAYMGHLDVVCLLVDQGAEVSCKDKRGYTPLHAAASNGQINVVKHLLNLSVEIDEANAFGNTALHVACFNGQDAVVSELIDYGANV 265          

HSP 4 Score: 46.2098 bits (108), Expect = 9.715e-7
Identity = 27/95 (28.42%), Postives = 47/95 (49.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            +I+AL    + P+H A   G  E + +LI     +   D      +H A ++   EAV++LI H  D+ A D+N   P+H A     ++  +++I
Sbjct:   33 DINALDAEKRTPLHAAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVRVLIRHSADVNARDKNWQTPLHVAAANKALRCAEVII 127          
BLAST of SMED30003476 vs. Ensembl Cavefish
Match: ankrd44 (ankyrin repeat domain 44 [Source:ZFIN;Acc:ZDB-GENE-070912-605])

HSP 1 Score: 60.4622 bits (145), Expect = 9.038e-12
Identity = 33/107 (30.84%), Postives = 54/107 (50.47%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI H  +++A  K  Q P+H A     +    ++I    ++ + D      +HHA   GH E V LL+    +I A D+ D R +H+A   GH+ +V LL+D   ++
Sbjct:   89 LIRHSADVNARDKNWQTPLHVAAANKALRCAEVIIPLLSSVNVSDRGGRTALHHAALNGHTEMVSLLLSKGANINAFDKKDGRALHWAAYMGHLDVVCLLVDQGAEV 195          

HSP 2 Score: 55.4546 bits (132), Expect = 5.217e-10
Identity = 28/89 (31.46%), Postives = 48/89 (53.93%), Query Frame = 1
Query:   55 IHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            +H A + GH E V++L+ +  NI   D  D R +H A   GH++ V LL+    ++   D+  + P+H A   G + +VK L++   +I
Sbjct:  140 LHHAALNGHTEMVSLLLSKGANINAFDKKDGRALHWAAYMGHLDVVCLLVDQGAEVSCKDKRGYTPLHAAASNGQINVVKHLLNLSVEI 228          

HSP 3 Score: 53.1434 bits (126), Expect = 3.868e-9
Identity = 32/107 (29.91%), Postives = 52/107 (48.60%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+    NI+A  K D R +H+A   GH++ V +L+ +   +   D   +  +H A S G I  VK L+    +I+  +      +H AC  G   +V  LIDY  ++
Sbjct:  155 LLSKGANINAFDKKDGRALHWAAYMGHLDVVCLLVDQGAEVSCKDKRGYTPLHAAASNGQINVVKHLLNLSVEIDEANAFGNTALHVACFNGQDAVVSELIDYGANV 261          

HSP 4 Score: 46.2098 bits (108), Expect = 1.120e-6
Identity = 27/95 (28.42%), Postives = 47/95 (49.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            +I+AL    + P+H A   G  E + +LI     +   D      +H A ++   EAV++LI H  D+ A D+N   P+H A     ++  +++I
Sbjct:   29 DINALDAEKRTPLHAAAFLGDAEIIELLILSGARVNAKDNMWLTPLHRAVASRSEEAVRVLIRHSADVNARDKNWQTPLHVAAANKALRCAEVII 123          
BLAST of SMED30003476 vs. Ensembl Cavefish
Match: psmd10 (proteasome 26S subunit, non-ATPase 10 [Source:NCBI gene;Acc:103037594])

HSP 1 Score: 58.5362 bits (140), Expect = 2.305e-11
Identity = 32/97 (32.99%), Postives = 49/97 (50.52%), Query Frame = 1
Query:   31 LGKYDQRP-IHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            +G  D R  +H+AC AGH   V  L+     +++ D   W  +H A SAG  E V+ LI     + +++QN   P+HYA      +I  LL++   D
Sbjct:   21 IGVEDHRTALHWACSAGHANIVQFLLDVGAEVDLKDDASWTPLHIAASAGREEIVRSLISKGAQLNSVNQNGCTPLHYAASKNQYEIALLLLENGAD 117          

HSP 2 Score: 46.9802 bits (110), Expect = 3.170e-7
Identity = 27/107 (25.23%), Postives = 47/107 (43.93%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+D    +D        P+H A  AG  E V  LI +   +  ++      +H+A S    E   LL+ +  D  A D+ +  P+H A   G+ ++++LL+      
Sbjct:   45 LLDVGAEVDLKDDASWTPLHIAASAGREEIVRSLISKGAQLNSVNQNGCTPLHYAASKNQYEIALLLLENGADPNATDKLESTPLHRASAKGNYRLIQLLLKQSAST 151          

HSP 3 Score: 46.2098 bits (108), Expect = 7.304e-7
Identity = 23/88 (26.14%), Postives = 43/88 (48.86%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYA 264
            L+++  + +A  K +  P+H A   G+   + +L+K+  +  I D      +H AC    +EA KLL+ H   I   ++ +  P+  A
Sbjct:  111 LLENGADPNATDKLESTPLHRASAKGNYRLIQLLLKQSASTNIQDSEGNTALHLACDEERVEAAKLLVEHGASIYIENKEEKTPLQIA 198          
BLAST of SMED30003476 vs. Ensembl Cavefish
Match: psmd10 (proteasome 26S subunit, non-ATPase 10 [Source:NCBI gene;Acc:103037594])

HSP 1 Score: 58.151 bits (139), Expect = 3.054e-11
Identity = 29/88 (32.95%), Postives = 45/88 (51.14%), Query Frame = 1
Query:   55 IHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            +H+AC AGH   V  L+     +++ D   W  +H A SAG  E V+ LI     + +++QN   P+HYA      +I  LL++   D
Sbjct:   44 LHWACSAGHANIVQFLLDVGAEVDLKDDASWTPLHIAASAGREEIVRSLISKGAQLNSVNQNGCTPLHYAASKNQYEIALLLLENGAD 131          

HSP 2 Score: 47.3654 bits (111), Expect = 3.263e-7
Identity = 27/107 (25.23%), Postives = 47/107 (43.93%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+D    +D        P+H A  AG  E V  LI +   +  ++      +H+A S    E   LL+ +  D  A D+ +  P+H A   G+ ++++LL+      
Sbjct:   59 LLDVGAEVDLKDDASWTPLHIAASAGREEIVRSLISKGAQLNSVNQNGCTPLHYAASKNQYEIALLLLENGADPNATDKLESTPLHRASAKGNYRLIQLLLKQSAST 165          

HSP 3 Score: 46.9802 bits (110), Expect = 4.274e-7
Identity = 23/88 (26.14%), Postives = 43/88 (48.86%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYA 264
            L+++  + +A  K +  P+H A   G+   + +L+K+  +  I D      +H AC    +EA KLL+ H   I   ++ +  P+  A
Sbjct:  125 LLENGADPNATDKLESTPLHRASAKGNYRLIQLLLKQSASTNIQDSEGNTALHLACDEERVEAAKLLVEHGASIYIENKEEKTPLQIA 212          
BLAST of SMED30003476 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003991.1 (pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1 gene:ENSPMAG00000003623.1 transcript:ENSPMAT00000003991.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 71.2478 bits (173), Expect = 3.379e-16
Identity = 36/89 (40.45%), Postives = 49/89 (55.06%), Query Frame = 1
Query:   55 IHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            +H A   GH E VN L+ +  NI   D  D R IH A   GH E VK+L+ H  D+   D+  + P+H A  +G + +VKLL+D   DI
Sbjct:  142 LHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDI 230          

HSP 2 Score: 59.3066 bits (142), Expect = 6.352e-12
Identity = 32/107 (29.91%), Postives = 53/107 (49.53%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     ++    +I    +  + D      +HHA   GH E V  L+    +I A D+ D R IH+A   GH ++VK+L+ +  D+
Sbjct:   91 LLKHSADVNARDKNWQTPLHVAAANRAVKCAEAIIPMLSSSNVSDRAGRTALHHAAFNGHSEMVNFLVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADV 197          

HSP 3 Score: 56.6102 bits (135), Expect = 4.315e-11
Identity = 35/102 (34.31%), Postives = 51/102 (50.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GH E V +L+    ++   D   +  +H A S+G I  VKLL+    DI+  +      +H AC  G   +V  LID
Sbjct:  157 LVAKGANINAFDKKDRRAIHWAAYMGHTEVVKVLVAHGADVRCKDKKGYSPLHAASSSGQIGVVKLLLDLGVDIDDPNSFGNTALHVACYNGQDVVVNELID 258          

HSP 4 Score: 48.521 bits (114), Expect = 2.887e-8
Identity = 27/99 (27.27%), Postives = 46/99 (46.46%), Query Frame = 1
Query:    7 DHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            D +  +DA    +Q P+  A   GH +  ++L+ +  +I   D      +H   + GH E V LL+    D+   ++    P+H A   GHM I+  L+
Sbjct:  674 DSNEAVDASDANEQTPVMLAVGNGHTDCASLLLSKGASINAKDNKGCTALHRGAATGHEECVDLLLQRSVDVSLRERRGRAPLHMAAACGHMGILSALV 772          

HSP 5 Score: 42.743 bits (99), Expect = 3.218e-6
Identity = 23/86 (26.74%), Postives = 46/86 (53.49%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWR-PIHYACCAGHMKIVKLLID 306
            P+H A   GH   + +LI+   ++++ +G  +  +  A   GH + V++LI H   +   +  D R  +H A   GH++ ++LL++
Sbjct:  586 PLHLAAYHGHHGALEVLIRSLVDLDVREGNGYTPLGLAALRGHTDCVRVLIQHGASVLLKEGPDRRTALHSAATNGHVECLRLLVE 671          
BLAST of SMED30003476 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007954.1 (pep scaffold:Pmarinus_7.0:GL479092:583:36301:1 gene:ENSPMAG00000007165.1 transcript:ENSPMAT00000007954.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 63.5438 bits (153), Expect = 2.008e-13
Identity = 30/101 (29.70%), Postives = 57/101 (56.44%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A   G++E VN+LI +  N+  +D  + R +H A   GH E VK+L+ +  D+   D+  + P+H A  +GH+ +V+LL+    ++
Sbjct:   39 NVNVSDRAGRTALHHAAFNGNLEMVNLLISKGANVNAMDKRERRAVHWAGYMGHTEVVKMLLSNGADVNWKDKKGYTPLHAAAASGHVTVVRLLLSLKAEM 139          

HSP 2 Score: 58.5362 bits (140), Expect = 9.359e-12
Identity = 32/104 (30.77%), Postives = 55/104 (52.88%), Query Frame = 1
Query:   10 HHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            H  +++A  K  Q P+H A     I+  + LI    N+ + D      +HHA   G++E V LLI    ++ A+D+ + R +H+A   GH ++VK+L+    D+
Sbjct:    3 HAADVNARDKNWQTPLHVASANRAIKCADALIPLLSNVNVSDRAGRTALHHAAFNGNLEMVNLLISKGANVNAMDKRERRAVHWAGYMGHTEVVKMLLSNGADV 106          

HSP 3 Score: 52.373 bits (124), Expect = 1.575e-9
Identity = 29/106 (27.36%), Postives = 53/106 (50.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            LI    N++A+ K ++R +H+A   GH E V +L+    ++   D   +  +H A ++GH+  V+LL+    ++   +      +H AC  G   +V  L+D   D
Sbjct:   66 LISKGANVNAMDKRERRAVHWAGYMGHTEVVKMLLSNGADVNWKDKKGYTPLHAAAASGHVTVVRLLLSLKAEMNEFNGFGNTALHVACHNGQDAVVSELLDAGAD 171          

HSP 4 Score: 43.1282 bits (100), Expect = 2.672e-6
Identity = 26/108 (24.07%), Postives = 52/108 (48.15%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKI-VKLLIDYHCDI 321
            L+ +  +++   K    P+H A  +GH+  V +L+     +   +GF    +H AC  G    V  L+    D    ++  +  +H+A  + H  I ++LL+++H D+
Sbjct:   99 LLSNGADVNWKDKKGYTPLHAAAASGHVTVVRLLLSLKAEMNEFNGFGNTALHVACHNGQDAVVSELLDAGADAGHRNERGFTALHFAAASTHGAICLELLVNHHADV 206          
BLAST of SMED30003476 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010488.1 (pep scaffold:Pmarinus_7.0:GL501102:192066:206968:-1 gene:ENSPMAG00000009500.1 transcript:ENSPMAT00000010488.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 56.9954 bits (136), Expect = 3.350e-11
Identity = 31/91 (34.07%), Postives = 44/91 (48.35%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            + P+H A I G I+ V  L++   N  I D   W  +H AC+ GH   V+LL+ H   I A    +  P+H A   GH  +  LL+    D
Sbjct:  383 ETPLHVAAIKGDIDGVEHLLENGANPNIKDNAGWTPLHEACNHGHARVVELLLDHGAFINAPGYENDSPLHDAVSNGHTHVAGLLVGRGAD 473          
BLAST of SMED30003476 vs. Ensembl Sea Lamprey
Match: ankrd16 (ankyrin repeat domain 16 [Source:ZFIN;Acc:ZDB-GENE-041210-253])

HSP 1 Score: 54.299 bits (129), Expect = 2.767e-10
Identity = 27/88 (30.68%), Postives = 47/88 (53.41%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACS-AGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            +RP+H A  AGH   V +L++    ++ L   DW  +  AC+ AG ++ V+ L+ H   +   +++ W  +H AC  G   +V  L+D
Sbjct:   40 KRPLHEAAAAGHGPCVRLLLRHGARVDSLKRADWTPLMMACTRAGSLDVVRELLAHGASLSVRNKDGWSALHVACRTGDSAVVGALLD 127          
BLAST of SMED30003476 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007332.1 (pep scaffold:Pmarinus_7.0:GL483392:1130:9476:1 gene:ENSPMAG00000006613.1 transcript:ENSPMAT00000007332.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.9138 bits (128), Expect = 4.256e-10
Identity = 28/87 (32.18%), Postives = 45/87 (51.72%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYN--IEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            P+H AC  GH     +L+       +  LD      +H A +AGH+E ++LL+    DI A D++   P+H A  AG +  V+ L++
Sbjct:   41 PLHLACQGGHAAVAGLLLSRAAPPLLRTLDQRGHSCLHLAATAGHVETLRLLLGQGADINATDKSGCTPLHVAARAGRLPAVRALLE 127          

HSP 2 Score: 41.5874 bits (96), Expect = 7.743e-6
Identity = 29/94 (30.85%), Postives = 41/94 (43.62%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRL--IHHACSAGHIEAVKLLIYHHCD--IEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            P+H A  AGH   V +L+      E L         +H AC  GH     LL+       +  LDQ     +H A  AGH++ ++LL+    DI
Sbjct:    6 PLHLAAQAGHESLVRLLLNHPSTQEDLQAGPQATTPLHLACQGGHAAVAGLLLSRAAPPLLRTLDQRGHSCLHLAATAGHVETLRLLLGQGADI 99          
BLAST of SMED30003476 vs. Ensembl Yeast
Match: GDE1 (Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes [Source:SGD;Acc:S000006031])

HSP 1 Score: 46.2098 bits (108), Expect = 1.443e-7
Identity = 24/86 (27.91%), Postives = 43/86 (50.00%), Query Frame = 1
Query:   46 QRPIHFACIAGHIETVNILIKEYYNIEILDG-FDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLL 300
            + P++ AC     E    L+    ++EI +  F W  I  A + G  + VKLLI ++ + +  D+  W P+ +A   GH+ I  ++
Sbjct:  540 ETPLYLACRLNFFEAAVCLLYNGADLEIREKLFGWTAIFVAAAEGFTDIVKLLIANNANFDIEDEGGWTPMEHAVLRGHLHIADMV 625          
BLAST of SMED30003476 vs. Ensembl Yeast
Match: PHO81 (Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000003465])

HSP 1 Score: 42.743 bits (99), Expect = 2.125e-6
Identity = 23/81 (28.40%), Postives = 42/81 (51.85%), Query Frame = 1
Query:   64 ACIAGHIETVNILIKEYYNIEILDGFD-WRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            A I G  + + +LI+   +   +DGF+ W  I +A  +GH E +  L+ H+  ++  D N   P+ YA    H+ ++  L+
Sbjct:  565 AKIGGDPQLIQLLIRYGADPNEIDGFNKWTPIFYAVRSGHSEVITELLKHNARLDIEDDNGHSPLFYALWESHVDVLNALL 645          
BLAST of SMED30003476 vs. Ensembl Yeast
Match: MGA2 (ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; MGA2 has a paralog, SPT23, that arose from the whole genome duplication [Source:SGD;Acc:S000001472])

HSP 1 Score: 42.743 bits (99), Expect = 2.599e-6
Identity = 21/63 (33.33%), Postives = 32/63 (50.79%), Query Frame = 1
Query:  118 NIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            NI I D     L+H AC   +   V  LI     +  +D     P+H+AC +G  KI+K+L++
Sbjct:  712 NISICDSLGRTLLHLACLKNYSSLVYTLIKKGARVNDIDSFGLTPLHFACISGDPKIIKMLLN 774          
BLAST of SMED30003476 vs. Ensembl Nematostella
Match: EDO46099 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR35])

HSP 1 Score: 59.3066 bits (142), Expect = 1.469e-12
Identity = 35/111 (31.53%), Postives = 59/111 (53.15%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWR---LIHHACSAGHIE-AVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+     ++   +Y  RPIH A + GH E V +L++   +     G   R    +H+A   GH++ AV++L+    D+EA+ ++ WR IH A   G +K++  L+D   DI
Sbjct:   16 LLKSGAKLNVPQRYGWRPIHEAAMNGHTECVRMLLRYGSHANAKTGKTIRNMTPLHYAARMGHLDCAVRMLMERGIDLEAVYRDGWRLIHVASDNGRVKVLVTLLDAGVDI 126          

HSP 2 Score: 47.7506 bits (112), Expect = 3.520e-8
Identity = 26/93 (27.96%), Postives = 47/93 (50.54%), Query Frame = 1
Query:   55 IHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWR---PIHYACCAGHMK-IVKLLIDYHCDI 321
            +H+A   G+ + + +L+K    + +   + WR IH A   GH E V++L+ +     A      R   P+HYA   GH+   V++L++   D+
Sbjct:    1 LHYAARYGNEKCIELLLKSGAKLNVPQRYGWRPIHEAAMNGHTECVRMLLRYGSHANAKTGKTIRNMTPLHYAARMGHLDCAVRMLMERGIDL 93          
BLAST of SMED30003476 vs. Ensembl Nematostella
Match: EDO34161 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQ63])

HSP 1 Score: 53.9138 bits (128), Expect = 7.510e-11
Identity = 25/78 (32.05%), Postives = 42/78 (53.85%), Query Frame = 1
Query:   85 ETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            E V   I +  +    D   W  +H AC+ G +E VK+L+ +  D+ A   +  RP+H A   GH+++++LL+ Y  D
Sbjct:    1 EVVKQCILQGADPTAKDNAGWTPLHEACARGKLEVVKVLVQYGADVNAHSNDGIRPLHDAAEGGHVEVLRLLLTYGAD 78          

HSP 2 Score: 43.1282 bits (100), Expect = 9.361e-7
Identity = 18/46 (39.13%), Postives = 25/46 (54.35%), Query Frame = 1
Query:  184 EAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            E VK  I    D  A D   W P+H AC  G +++VK+L+ Y  D+
Sbjct:    1 EVVKQCILQGADPTAKDNAGWTPLHEACARGKLEVVKVLVQYGADV 46          
BLAST of SMED30003476 vs. Ensembl Nematostella
Match: EDO34162 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQ64])

HSP 1 Score: 52.373 bits (124), Expect = 2.531e-10
Identity = 25/78 (32.05%), Postives = 41/78 (52.56%), Query Frame = 1
Query:   85 ETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCD 318
            E V   I +  +    D   W  +H AC+ G +E VK+L  +  D+ A   +  RP+H A   GH+++++LL+ Y  D
Sbjct:    1 EVVKQCILQGADPTAKDNAGWTPLHEACARGKLEVVKVLAQYGADVNAHSNDGIRPLHDAAEGGHVEVLRLLLTYGAD 78          

HSP 2 Score: 41.9726 bits (97), Expect = 3.050e-6
Identity = 18/46 (39.13%), Postives = 24/46 (52.17%), Query Frame = 1
Query:  184 EAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            E VK  I    D  A D   W P+H AC  G +++VK+L  Y  D+
Sbjct:    1 EVVKQCILQGADPTAKDNAGWTPLHEACARGKLEVVKVLAQYGADV 46          
BLAST of SMED30003476 vs. Ensembl Nematostella
Match: EDO34704 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNN1])

HSP 1 Score: 50.8322 bits (120), Expect = 1.055e-9
Identity = 28/91 (30.77%), Postives = 48/91 (52.75%), Query Frame = 1
Query:   37 KYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHC--DIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            +Y + P+H A  A ++E V +L++   N+ +LD  +   +H A   G I  + LL+       +  LD+N+  P+H A   GH+ IV  L+
Sbjct:    6 EYGKTPLHCAAHAQNVEAVELLLERRANVNVLDDREETPLHAAVRTGKIAVINLLLEGSNVRMVNWLDKNERTPLHLAAKHGHVDIVDFLL 96          
BLAST of SMED30003476 vs. Ensembl Nematostella
Match: EDO42168 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S234])

HSP 1 Score: 49.2914 bits (116), Expect = 2.755e-9
Identity = 24/81 (29.63%), Postives = 42/81 (51.85%), Query Frame = 1
Query:   52 PIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVK 294
            P+H A   G+IE   +LI+   N+  ++GF    +H+AC A  +  V +L+ +  D   LD      +H A   GH+ +++
Sbjct:    4 PLHRAATRGNIEACKLLIQYGANVNKVNGFGETPLHYACQAASLRFVNVLVENGADFNVLDNGGRSFLHIAAEHGHIDLLE 84          

HSP 2 Score: 45.4394 bits (106), Expect = 9.539e-8
Identity = 19/56 (33.93%), Postives = 33/56 (58.93%), Query Frame = 1
Query:  154 IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            +H A + G+IEA KLLI +  ++  ++     P+HYAC A  ++ V +L++   D 
Sbjct:    5 LHRAATRGNIEACKLLIQYGANVNKVNGFGETPLHYACQAASLRFVNVLVENGADF 60          

HSP 3 Score: 43.5134 bits (101), Expect = 5.759e-7
Identity = 18/62 (29.03%), Postives = 34/62 (54.84%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIE 186
            LI +  N++ +  + + P+H+AC A  +  VN+L++   +  +LD      +H A   GHI+
Sbjct:   20 LIQYGANVNKVNGFGETPLHYACQAASLRFVNVLVENGADFNVLDNGGRSFLHIAAEHGHID 81          
BLAST of SMED30003476 vs. Ensembl Medaka
Match: ankrd28b (ankyrin repeat domain 28 [Source:NCBI gene;Acc:101159131])

HSP 1 Score: 71.2478 bits (173), Expect = 1.559e-15
Identity = 36/101 (35.64%), Postives = 54/101 (53.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH+E V +L+    NI   D  D R IH A   GHIE VKLL  H  ++   D+  + P+H A  +G + +VK L+D   DI
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDI 232          

HSP 2 Score: 67.0106 bits (162), Expect = 4.872e-14
Identity = 34/107 (31.78%), Postives = 57/107 (53.27%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     +     L+    N+ + D      +HHA  +GH+E V+LL+    +I A D+ D R IH+A   GH+++VKLL  +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHIAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEV 199          

HSP 3 Score: 56.6102 bits (135), Expect = 2.613e-10
Identity = 35/102 (34.31%), Postives = 49/102 (48.04%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L      +   D   +  +H A S+G I  VK L+    DI   +     P+H AC  G   +V  LI+
Sbjct:  159 LLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNTPLHVACYNGQDVVVNELIE 260          
BLAST of SMED30003476 vs. Ensembl Medaka
Match: ankrd28b (ankyrin repeat domain 28 [Source:NCBI gene;Acc:101159131])

HSP 1 Score: 71.2478 bits (173), Expect = 1.932e-15
Identity = 36/101 (35.64%), Postives = 54/101 (53.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH+E V +L+    NI   D  D R IH A   GHIE VKLL  H  ++   D+  + P+H A  +G + +VK L+D   DI
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDI 232          

HSP 2 Score: 67.0106 bits (162), Expect = 5.809e-14
Identity = 34/107 (31.78%), Postives = 57/107 (53.27%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     +     L+    N+ + D      +HHA  +GH+E V+LL+    +I A D+ D R IH+A   GH+++VKLL  +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHIAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEV 199          

HSP 3 Score: 56.225 bits (134), Expect = 3.086e-10
Identity = 35/102 (34.31%), Postives = 49/102 (48.04%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L      +   D   +  +H A S+G I  VK L+    DI   +     P+H AC  G   +V  LI+
Sbjct:  159 LLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNTPLHVACYNGQDVVVNELIE 260          

HSP 4 Score: 44.669 bits (104), Expect = 3.493e-6
Identity = 26/106 (24.53%), Postives = 54/106 (50.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEAL----DQNDWRPIHYACCAGHMKIVKLLID 306
            L++   +++A  K+ +  +H   + GH E +  L++   +  + D      +H A + GHI  +  L++    +E L    D + + P+H+AC  GH   V++L++
Sbjct:  672 LLNKGASVEAKDKWGRTALHRGAVTGHEECMEALLQHNASFLVRDCRGRTPVHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGHDTCVEVLLE 777          
BLAST of SMED30003476 vs. Ensembl Medaka
Match: ankrd28b (ankyrin repeat domain 28 [Source:NCBI gene;Acc:101159131])

HSP 1 Score: 70.8626 bits (172), Expect = 2.742e-15
Identity = 36/101 (35.64%), Postives = 54/101 (53.47%), Query Frame = 1
Query:   19 NIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            N++   +  +  +H A  +GH+E V +L+    NI   D  D R IH A   GHIE VKLL  H  ++   D+  + P+H A  +G + +VK L+D   DI
Sbjct:  132 NVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDI 232          

HSP 2 Score: 66.6254 bits (161), Expect = 8.085e-14
Identity = 34/107 (31.78%), Postives = 57/107 (53.27%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L+ H  +++A  K  Q P+H A     +     L+    N+ + D      +HHA  +GH+E V+LL+    +I A D+ D R IH+A   GH+++VKLL  +  ++
Sbjct:   93 LLKHSADVNARDKNWQTPLHIAAANKAVRCAEALVPLLSNVNVSDRAGRTALHHAAFSGHLEMVRLLLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEV 199          

HSP 3 Score: 55.8398 bits (133), Expect = 4.092e-10
Identity = 35/102 (34.31%), Postives = 49/102 (48.04%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            L+    NI+A  K D+R IH+A   GHIE V +L      +   D   +  +H A S+G I  VK L+    DI   +     P+H AC  G   +V  LI+
Sbjct:  159 LLSRGSNINAFDKKDRRAIHWAAYMGHIEVVKLLASHGAEVTCKDKKSYTPLHAAASSGMISVVKFLLDLGVDINEPNAYGNTPLHVACYNGQDVVVNELIE 260          

HSP 4 Score: 44.2838 bits (103), Expect = 4.454e-6
Identity = 26/106 (24.53%), Postives = 54/106 (50.94%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEAL----DQNDWRPIHYACCAGHMKIVKLLID 306
            L++   +++A  K+ +  +H   + GH E +  L++   +  + D      +H A + GHI  +  L++    +E L    D + + P+H+AC  GH   V++L++
Sbjct:  672 LLNKGASVEAKDKWGRTALHRGAVTGHEECMEALLQHNASFLVRDCRGRTPVHLAAACGHIGVLGGLLHAAQSVETLPVLTDSHGYTPLHWACYNGHDTCVEVLLE 777          
BLAST of SMED30003476 vs. Ensembl Medaka
Match: ENSORLT00000036692.1 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C [Source:NCBI gene;Acc:101169196])

HSP 1 Score: 68.9366 bits (167), Expect = 1.299e-14
Identity = 34/101 (33.66%), Postives = 58/101 (57.43%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            LI H C++D   K  + P+H+A  +GH E VN+L+ +  N+   D  + + IH A   G++E VKLL+ H   +   D++ + P+H A  +G + +V  L+
Sbjct:  126 LIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSHGASVTCKDKHGYTPLHVAAVSGQLDVVSYLL 226          

HSP 2 Score: 55.0694 bits (131), Expect = 9.082e-10
Identity = 28/103 (27.18%), Postives = 54/103 (52.43%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            L+ H   +++  K+   P+H A           LI    ++++ D      +H+A  +GH E V LL+    ++ A D+ + +PIH+A   G++++VKLL+ +
Sbjct:   93 LLKHKSEVNSRDKFWHTPLHVAAANWATSCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSH 195          
BLAST of SMED30003476 vs. Ensembl Medaka
Match: ENSORLT00000018386.2 (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C [Source:NCBI gene;Acc:101169196])

HSP 1 Score: 68.5514 bits (166), Expect = 1.377e-14
Identity = 34/101 (33.66%), Postives = 58/101 (57.43%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            LI H C++D   K  + P+H+A  +GH E VN+L+ +  N+   D  + + IH A   G++E VKLL+ H   +   D++ + P+H A  +G + +V  L+
Sbjct:  122 LIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSHGASVTCKDKHGYTPLHVAAVSGQLDVVSYLL 222          

HSP 2 Score: 54.6842 bits (130), Expect = 9.717e-10
Identity = 28/103 (27.18%), Postives = 54/103 (52.43%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDY 309
            L+ H   +++  K+   P+H A           LI    ++++ D      +H+A  +GH E V LL+    ++ A D+ + +PIH+A   G++++VKLL+ +
Sbjct:   89 LLKHKSEVNSRDKFWHTPLHVAAANWATSCAAALIPHVCSLDVTDKLGRTPLHYAAHSGHREMVNLLVCKGANVSAKDKKERQPIHWAAHLGYLEVVKLLVSH 191          
BLAST of SMED30003476 vs. Planmine SMEST
Match: SMESG000032703.1 (SMESG000032703.1)

HSP 1 Score: 204.527 bits (519), Expect = 1.087e-62
Identity = 107/140 (76.43%), Postives = 107/140 (76.43%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHH---------------------------------ACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHH                                 ACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI
Sbjct:  134 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIETMKLLIDHHCDIEAFDWRNKRSIHLACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 273          

HSP 2 Score: 135.191 bits (339), Expect = 5.259e-38
Identity = 68/107 (63.55%), Postives = 82/107 (76.64%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LID+HC+I+A    D RPIHFA  AGHIET+ +LI  + +IE+ D    R IH AC AGHIE +KLLIYHHCDI+ALDQND+RPIH+A   GH + VKLLIDYHCD+
Sbjct:  299 LIDYHCDINASDMNDNRPIHFALRAGHIETMKLLIDHHCDIEVFDRHKQRPIHLACIAGHIETMKLLIYHHCDIDALDQNDYRPIHFALREGHTEAVKLLIDYHCDV 405          

HSP 3 Score: 128.642 bits (322), Expect = 8.856e-36
Identity = 61/107 (57.01%), Postives = 83/107 (77.57%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LID+HC++DA  K++ RPIH  C +GHIE   +LI  + +IE  D   +R IHHAC +GHIE VKLLI HHCDI+A D+NDW+PIH+AC AG+++ +KLLID++C+I
Sbjct:  398 LIDYHCDVDAPDKWNIRPIHLTCRSGHIEVSKLLIDHHCDIEAPDELGFRPIHHACRSGHIETVKLLIDHHCDIDASDRNDWQPIHHACVAGNIETLKLLIDHYCNI 504          

HSP 4 Score: 125.176 bits (313), Expect = 1.409e-34
Identity = 65/107 (60.75%), Postives = 81/107 (75.70%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI HHC+I+AL + D RPIH+AC AGH++ V +LI  + +I+  D  DWR IH AC AGHIE VKLLI +HCDI A D ND RPIH+A  AGH++ +KLLID+HCDI
Sbjct:  233 LIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDIDASDTNDWRPIHLACGAGHIEIVKLLIDYHCDINASDMNDNRPIHFALRAGHIETMKLLIDHHCDI 339          

HSP 5 Score: 123.635 bits (309), Expect = 6.221e-34
Identity = 61/107 (57.01%), Postives = 81/107 (75.70%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDHHC+I+   ++ QRPIH ACIAGHIET+ +LI  + +I+ LD  D+R IH A   GH EAVKLLI +HCD++A D+ + RPIH  C +GH+++ KLLID+HCDI
Sbjct:  332 LIDHHCDIEVFDRHKQRPIHLACIAGHIETMKLLIYHHCDIDALDQNDYRPIHFALREGHTEAVKLLIDYHCDVDAPDKWNIRPIHLTCRSGHIEVSKLLIDHHCDI 438          

HSP 6 Score: 118.242 bits (295), Expect = 3.853e-32
Identity = 61/107 (57.01%), Postives = 78/107 (72.90%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI HHC+IDAL + D RPIHFA   GH E V +LI  + +++  D ++ R IH  C +GHIE  KLLI HHCDIEA D+  +RPIH+AC +GH++ VKLLID+HCDI
Sbjct:  365 LIYHHCDIDALDQNDYRPIHFALREGHTEAVKLLIDYHCDVDAPDKWNIRPIHLTCRSGHIEVSKLLIDHHCDIEAPDELGFRPIHHACRSGHIETVKLLIDHHCDI 471          

HSP 7 Score: 115.931 bits (289), Expect = 2.558e-31
Identity = 63/107 (58.88%), Postives = 75/107 (70.09%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LID+HC+IDA    D RPIH AC AGHIE V +LI  + +I   D  D R IH A  AGHIE +KLLI HHCDIE  D++  RPIH AC AGH++ +KLLI +HCDI
Sbjct:  266 LIDYHCDIDASDTNDWRPIHLACGAGHIEIVKLLIDYHCDINASDMNDNRPIHFALRAGHIETMKLLIDHHCDIEVFDRHKQRPIHLACIAGHIETMKLLIYHHCDI 372          

HSP 8 Score: 115.931 bits (289), Expect = 2.609e-31
Identity = 62/123 (50.41%), Postives = 82/123 (66.67%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRL----------------IHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LID+HCNI+A  + + RPIH AC AG+ E V +LI  + +I +LDG +W +                IHH C+A H+E VKLLI HHCDIE+LD +  RPIH+AC  G M++VKLLID+HC+I
Sbjct:   19 LIDYHCNIEASDESNWRPIHHACSAGNTEAVKLLINCHPSIWVLDGSEWPIIQRVCEKPLDRSNRQPIHHTCNARHVETVKLLIDHHCDIESLDNSGGRPIHHACIGGCMEVVKLLIDHHCNI 141          

HSP 9 Score: 114.775 bits (286), Expect = 6.250e-31
Identity = 60/107 (56.07%), Postives = 76/107 (71.03%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDHHC+I++L     RPIH ACI G +E V +LI  + NI+ L  +D R IH AC AGHIE V +LI  + +IE LD  DWR IH+AC AGH++ +KLLID+HCDI
Sbjct:  101 LIDHHCDIESLDNSGGRPIHHACIGGCMEVVKLLIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIETMKLLIDHHCDI 207          

HSP 10 Score: 96.2857 bits (238), Expect = 1.994e-24
Identity = 62/159 (38.99%), Postives = 80/159 (50.31%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEAL----------------------------------------------------DQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDHHC+I+A  +   RPIH AC +GHIETV +LI  + +I+  D  DW+ IHHAC AG+IE +KLLI H+C+IEAL                                                    D+   RPIH+AC +G++KIVK LI   CDI
Sbjct:  431 LIDHHCDIEAPDELGFRPIHHACRSGHIETVKLLIDHHCDIDASDRNDWQPIHHACVAGNIETLKLLIDHYCNIEALDKFGDSPIEIAVRSCNEGIIHLLNGARLLLACKTGDTKKVSRLLNSITIEYCDKGKRRPIHWACESGNIKIVKSLIKTKCDI 589          

HSP 11 Score: 94.3597 bits (233), Expect = 1.135e-23
Identity = 49/98 (50.00%), Postives = 67/98 (68.37%), Query Frame = 1
Query:   28 ALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
             L + +++PIH  C A H+ETV +LI  + +IE LD    R IHHAC  G +E VKLLI HHC+I+AL + D RPIH+AC AGH++ V +LI  + +I
Sbjct:   77 PLDRSNRQPIHHTCNARHVETVKLLIDHHCDIESLDNSGGRPIHHACIGGCMEVVKLLIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNI 174          

HSP 12 Score: 91.2781 bits (225), Expect = 1.192e-22
Identity = 53/107 (49.53%), Postives = 64/107 (59.81%), Query Frame = 1
Query:   49 RPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDW----------------RPIHYACCAGHMKIVKLLIDYHCDI 321
            RPIH AC    IE V +LI  + NIE  D  +WR IHHACSAG+ EAVKLLI  H  I  LD ++W                +PIH+ C A H++ VKLLID+HCDI
Sbjct:    2 RPIHHACSENQIEIVKLLIDYHCNIEASDESNWRPIHHACSAGNTEAVKLLINCHPSIWVLDGSEWPIIQRVCEKPLDRSNRQPIHHTCNARHVETVKLLIDHHCDI 108          

HSP 13 Score: 71.2478 bits (173), Expect = 1.100e-15
Identity = 50/152 (32.89%), Postives = 69/152 (45.39%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGF----------------------------------------------------DWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLL 300
            LIDHHC+IDA  + D +PIH AC+AG+IET+ +LI  Y NIE LD F                                                      R IH AC +G+I+ VK LI   CDI A D+++ RP+  A    + ++V++L
Sbjct:  464 LIDHHCDIDASDRNDWQPIHHACVAGNIETLKLLIDHYCNIEALDKFGDSPIEIAVRSCNEGIIHLLNGARLLLACKTGDTKKVSRLLNSITIEYCDKGKRRPIHWACESGNIKIVKSLIKTKCDITAKDKDNLRPLDIAIKKENKELVEIL 615          
BLAST of SMED30003476 vs. Planmine SMEST
Match: SMESG000010485.1 (SMESG000010485.1)

HSP 1 Score: 125.176 bits (313), Expect = 1.134e-36
Identity = 62/107 (57.94%), Postives = 80/107 (74.77%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L++H+CNI+AL  ++QRPIH AC  GH E V +LI+   +IE  D  + R IHHAC  GH EA+KLLI H+CDIEALD ++WRPIH AC  GH + VKLLI+++CDI
Sbjct:   83 LLEHNCNIEALDIWNQRPIHLACYQGHSEAVKLLIEYNCDIEAFDDRNQRPIHHACYQGHSEAIKLLIEHNCDIEALDIDNWRPIHLACYQGHSEAVKLLIEHNCDI 189          

HSP 2 Score: 120.168 bits (300), Expect = 1.034e-34
Identity = 60/107 (56.07%), Postives = 80/107 (74.77%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI+H+C+I+AL   +QRPIH ACI GH ET+ +L++   NIE LD ++ R IH AC  GH EAVKLLI ++CDIEA D  + RPIH+AC  GH + +KLLI+++CDI
Sbjct:   50 LIEHNCDIEALDGLNQRPIHLACIQGHSETIKLLLEHNCNIEALDIWNQRPIHLACYQGHSEAVKLLIEYNCDIEAFDDRNQRPIHHACYQGHSEAIKLLIEHNCDI 156          

HSP 3 Score: 117.087 bits (292), Expect = 1.441e-33
Identity = 60/107 (56.07%), Postives = 78/107 (72.90%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI+++C+I+A    +QRPIH AC  GH E + +LI+   +IE LD  +WR IH AC  GH EAVKLLI H+CDIEALD+   RPIH+AC  G+++IVKLLI+  CDI
Sbjct:  116 LIEYNCDIEAFDDRNQRPIHHACYQGHSEAIKLLIEHNCDIEALDIDNWRPIHLACYQGHSEAVKLLIEHNCDIEALDKRKQRPIHWACENGNIEIVKLLIERKCDI 222          

HSP 4 Score: 112.464 bits (280), Expect = 1.002e-31
Identity = 58/107 (54.21%), Postives = 76/107 (71.03%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L++H+C+I+AL K + RPIH AC  GH E   +LI+   +IE LDG + R IH AC  GH E +KLL+ H+C+IEALD  + RPIH AC  GH + VKLLI+Y+CDI
Sbjct:   17 LLEHNCDIEALYKNNWRPIHHACYQGHSEAAKLLIEHNCDIEALDGLNQRPIHLACIQGHSETIKLLLEHNCNIEALDIWNQRPIHLACYQGHSEAVKLLIEYNCDI 123          

HSP 5 Score: 82.0333 bits (201), Expect = 3.783e-20
Identity = 41/78 (52.56%), Postives = 55/78 (70.51%), Query Frame = 1
Query:   88 TVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
             V +L++   +IE L   +WR IHHAC  GH EA KLLI H+CDIEALD  + RPIH AC  GH + +KLL++++C+I
Sbjct:   13 AVKLLLEHNCDIEALYKNNWRPIHHACYQGHSEAAKLLIEHNCDIEALDGLNQRPIHLACIQGHSETIKLLLEHNCNI 90          

HSP 6 Score: 80.8777 bits (198), Expect = 1.014e-19
Identity = 46/100 (46.00%), Postives = 62/100 (62.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLL 300
            LI+H+C+I+AL   + RPIH AC  GH E V +LI+   +IE LD    R IH AC  G+IE VKLLI   CDI A D+ +  P+  A    + K+V++L
Sbjct:  149 LIEHNCDIEALDIDNWRPIHLACYQGHSEAVKLLIEHNCDIEALDKRKQRPIHWACENGNIEIVKLLIERKCDITARDKCEMSPLDIAVKNENKKLVEIL 248          
BLAST of SMED30003476 vs. Planmine SMEST
Match: SMESG000010485.1 (SMESG000010485.1)

HSP 1 Score: 124.405 bits (311), Expect = 2.515e-36
Identity = 62/107 (57.94%), Postives = 80/107 (74.77%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI+H+C+I+AL  ++QRPIH AC  GH E V +LI+   +IE  D  + R IHHAC  GH EA+KLLI H+CDIEALD ++WRPIH AC  GH + VKLLI+++CDI
Sbjct:   88 LIEHNCDIEALDGWNQRPIHLACYQGHSEAVKLLIEYNCDIEAFDDRNQRPIHHACYQGHSEAIKLLIEHNCDIEALDIDNWRPIHLACYQGHSEAVKLLIEHNCDI 194          

HSP 2 Score: 116.701 bits (291), Expect = 2.642e-33
Identity = 60/107 (56.07%), Postives = 78/107 (72.90%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LI+++C+I+A    +QRPIH AC  GH E + +LI+   +IE LD  +WR IH AC  GH EAVKLLI H+CDIEALD+   RPIH+AC  G+++IVKLLI+  CDI
Sbjct:  121 LIEYNCDIEAFDDRNQRPIHHACYQGHSEAIKLLIEHNCDIEALDIDNWRPIHLACYQGHSEAVKLLIEHNCDIEALDKRKQRPIHWACENGNIEIVKLLIERKCDI 227          

HSP 3 Score: 114.005 bits (284), Expect = 2.570e-32
Identity = 58/107 (54.21%), Postives = 78/107 (72.90%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L++H+C+I+AL K + RPIH AC  GH E   +LI+   +IE LDG++ R IH AC  GH EAVKLLI ++CDIEA D  + RPIH+AC  GH + +KLLI+++CDI
Sbjct:   55 LLEHNCDIEALYKNNWRPIHHACYQGHSEAAKLLIEHNCDIEALDGWNQRPIHLACYQGHSEAVKLLIEYNCDIEAFDDRNQRPIHHACYQGHSEAIKLLIEHNCDI 161          

HSP 4 Score: 103.219 bits (256), Expect = 3.694e-28
Identity = 58/110 (52.73%), Postives = 71/110 (64.55%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQ---RPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            L D + N +   + D    RPIH ACI GH   V +L++   +IE L   +WR IHHAC  GH EA KLLI H+CDIEALD  + RPIH AC  GH + VKLLI+Y+CDI
Sbjct:   19 LFDRYFNAEFTEREDANGWRPIHHACIHGHSGAVKLLLEHNCDIEALYKNNWRPIHHACYQGHSEAAKLLIEHNCDIEALDGWNQRPIHLACYQGHSEAVKLLIEYNCDI 128          

HSP 5 Score: 80.4925 bits (197), Expect = 1.858e-19
Identity = 46/100 (46.00%), Postives = 62/100 (62.00%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLL 300
            LI+H+C+I+AL   + RPIH AC  GH E V +LI+   +IE LD    R IH AC  G+IE VKLLI   CDI A D+ +  P+  A    + K+V++L
Sbjct:  154 LIEHNCDIEALDIDNWRPIHLACYQGHSEAVKLLIEHNCDIEALDKRKQRPIHWACENGNIEIVKLLIERKCDITARDKCEMSPLDIAVKNENKKLVEIL 253          
BLAST of SMED30003476 vs. Planmine SMEST
Match: SMESG000017196.1 (SMESG000017196.1)

HSP 1 Score: 130.183 bits (326), Expect = 2.853e-36
Identity = 67/107 (62.62%), Postives = 80/107 (74.77%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDHHCNI+A  +   RPIH+AC+A HIE V +LI  + +IE LD +  R IH+ACSA H EAVKLLI HHCDIEA D+  WRPIH+AC  G ++ V LLIDYHCDI
Sbjct:  235 LIDHHCNIEASDRSGWRPIHYACMARHIEIVKLLIDYHCDIEALDRWKRRPIHYACSADHKEAVKLLITHHCDIEAFDEWGWRPIHHACHLGSIETVNLLIDYHCDI 341          

HSP 2 Score: 119.398 bits (298), Expect = 1.732e-32
Identity = 62/107 (57.94%), Postives = 73/107 (68.22%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDH+C+I+A+     RPIH AC  GH E V +LI  + +IE LD    R IHHAC  GH E VKLLI H+CDIEA D++ WRPIH+A   G   IVKLLID HCDI
Sbjct:   55 LIDHNCDIEAIDALGLRPIHHACRIGHTEIVKLLIDHFCDIEALDALGLRPIHHACRIGHTEIVKLLIDHYCDIEACDESGWRPIHHATNKGQTGIVKLLIDCHCDI 161          

HSP 3 Score: 112.079 bits (279), Expect = 5.473e-30
Identity = 63/121 (52.07%), Postives = 78/121 (64.46%), Query Frame = 1
Query:    1 LIDHHCNIDALG--------------KYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDHHC+++A                ++ +RPIH+AC   +IE V +LI  + NIE  D   WR IH+AC A HIE VKLLI +HCDIEALD+   RPIHYAC A H + VKLLI +HCDI
Sbjct:  188 LIDHHCDVEARDNVGGQFIMHAKKSDEWKRRPIHYACREEYIELVKLLIDHHCNIEASDRSGWRPIHYACMARHIEIVKLLIDYHCDIEALDRWKRRPIHYACSADHKEAVKLLITHHCDI 308          

HSP 4 Score: 107.071 bits (266), Expect = 3.208e-28
Identity = 61/109 (55.96%), Postives = 72/109 (66.06%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWR--PIHYACCAGHMKIVKLLIDYHCDI 321
            LIDH C+I+AL     RPIH AC  GH E V +LI  Y +IE  D   WR IHHA + G    VKLLI  HCDIE+ D N W   PIH+AC  GH++IVKLLID+HCD+
Sbjct:   88 LIDHFCDIEALDALGLRPIHHACRIGHTEIVKLLIDHYCDIEACDESGWRPIHHATNKGQTGIVKLLIDCHCDIESRD-NWWNRMPIHHACSVGHIEIVKLLIDHHCDV 195          

HSP 5 Score: 104.76 bits (260), Expect = 2.153e-27
Identity = 56/91 (61.54%), Postives = 64/91 (70.33%), Query Frame = 1
Query:   49 RPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            RPIH AC AGHI+ V +LI    +IE +D    R IHHAC  GH E VKLLI H CDIEALD    RPIH+AC  GH +IVKLLID++CDI
Sbjct:   38 RPIHHACSAGHIDIVKLLIDHNCDIEAIDALGLRPIHHACRIGHTEIVKLLIDHFCDIEALDALGLRPIHHACRIGHTEIVKLLIDHYCDI 128          

HSP 6 Score: 98.2117 bits (243), Expect = 4.331e-25
Identity = 51/101 (50.50%), Postives = 69/101 (68.32%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            LID+HC+I+AL ++ +RPIH+AC A H E V +LI  + +IE  D + WR IHHAC  G IE V LLI +HCDI ALD++   PI  A   G+ +I+ +LI
Sbjct:  268 LIDYHCDIEALDRWKRRPIHYACSADHKEAVKLLITHHCDIEAFDEWGWRPIHHACHLGSIETVNLLIDYHCDIGALDKSGNSPIEIAVRYGNEEIIHVLI 368          

HSP 7 Score: 58.5362 bits (140), Expect = 4.035e-11
Identity = 41/121 (33.88%), Postives = 60/121 (49.59%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILI----------------KEYYN---IEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            LID+HC+I AL K    PI  A   G+ E +++LI                 ++ N   IE  D    R IH AC +G+IE VKLLI   C I   D++D  P+  A    + ++V+++ D
Sbjct:  334 LIDYHCDIGALDKSGNSPIEIAVRYGNEEIIHVLIGPCLLLACTNGDLEKVSKFLNGSTIEYCDKVQRRPIHWACESGNIELVKLLIKSKCKITIKDKSDLSPLDIARRNDNKELVQVIKD 454          
BLAST of SMED30003476 vs. Planmine SMEST
Match: SMESG000017196.1 (SMESG000017196.1)

HSP 1 Score: 129.798 bits (325), Expect = 3.387e-36
Identity = 67/107 (62.62%), Postives = 80/107 (74.77%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDHHCNI+A  +   RPIH+AC+A HIE V +LI  + +IE LD +  R IH+ACSA H EAVKLLI HHCDIEA D+  WRPIH+AC  G ++ V LLIDYHCDI
Sbjct:  254 LIDHHCNIEASDRSGWRPIHYACMARHIEIVKLLIDYHCDIEALDRWKRRPIHYACSADHKEAVKLLITHHCDIEAFDEWGWRPIHHACHLGSIETVNLLIDYHCDI 360          

HSP 2 Score: 121.324 bits (303), Expect = 3.057e-33
Identity = 64/107 (59.81%), Postives = 78/107 (72.90%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDHHC+I+A  ++ +RPIH+AC   +IE V +LI  + NIE  D   WR IH+AC A HIE VKLLI +HCDIEALD+   RPIHYAC A H + VKLLI +HCDI
Sbjct:  221 LIDHHCDIEAGDEWKRRPIHYACREEYIELVKLLIDHHCNIEASDRSGWRPIHYACMARHIEIVKLLIDYHCDIEALDRWKRRPIHYACSADHKEAVKLLITHHCDI 327          

HSP 3 Score: 119.013 bits (297), Expect = 2.137e-32
Identity = 62/107 (57.94%), Postives = 73/107 (68.22%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDH+C+I+A+     RPIH AC  GH E V +LI  + +IE LD    R IHHAC  GH E VKLLI H+CDIEA D++ WRPIH+A   G   IVKLLID HCDI
Sbjct:   55 LIDHNCDIEAIDALGLRPIHHACRIGHTEIVKLLIDHFCDIEALDALGLRPIHHACRIGHTEIVKLLIDHYCDIEACDESGWRPIHHATNKGQTGIVKLLIDCHCDI 161          

HSP 4 Score: 115.931 bits (289), Expect = 2.523e-31
Identity = 60/107 (56.07%), Postives = 76/107 (71.03%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDHHC+++A      RPIH AC    I+ + +LI  + +IE  D +  R IH+AC   +IE VKLLI HHC+IEA D++ WRPIHYAC A H++IVKLLIDYHCDI
Sbjct:  188 LIDHHCDVEARDNSGWRPIHHACKEKFIDIIKLLIDHHCDIEAGDEWKRRPIHYACREEYIELVKLLIDHHCNIEASDRSGWRPIHYACMARHIEIVKLLIDYHCDI 294          

HSP 5 Score: 108.997 bits (271), Expect = 7.711e-29
Identity = 65/141 (46.10%), Postives = 75/141 (53.19%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWR----------------------------------LIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LIDH C+I+AL     RPIH AC  GH E V +LI  Y +IE  D   WR                                   IHHACS GHIE VKLLI HHCD+EA D + WRPIH+AC    + I+KLLID+HCDI
Sbjct:   88 LIDHFCDIEALDALGLRPIHHACRIGHTEIVKLLIDHYCDIEACDESGWRPIHHATNKGQTGIVKLLIDCHCDIESRDNWWNRMPIHHACSVGHIEIVKLLIDHHCDVEARDNSGWRPIHHACKEKFIDIIKLLIDHHCDI 228          

HSP 6 Score: 105.145 bits (261), Expect = 1.676e-27
Identity = 56/108 (51.85%), Postives = 75/108 (69.44%), Query Frame = 1
Query:    1 LIDHHCNIDALGKY-DQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            LID HC+I++   + ++ PIH AC  GHIE V +LI  + ++E  D   WR IHHAC    I+ +KLLI HHCDIEA D+   RPIHYAC   ++++VKLLID+HC+I
Sbjct:  154 LIDCHCDIESRDNWWNRMPIHHACSVGHIEIVKLLIDHHCDVEARDNSGWRPIHHACKEKFIDIIKLLIDHHCDIEAGDEWKRRPIHYACREEYIELVKLLIDHHCNI 261          

HSP 7 Score: 104.375 bits (259), Expect = 2.620e-27
Identity = 56/91 (61.54%), Postives = 64/91 (70.33%), Query Frame = 1
Query:   49 RPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLIDYHCDI 321
            RPIH AC AGHI+ V +LI    +IE +D    R IHHAC  GH E VKLLI H CDIEALD    RPIH+AC  GH +IVKLLID++CDI
Sbjct:   38 RPIHHACSAGHIDIVKLLIDHNCDIEAIDALGLRPIHHACRIGHTEIVKLLIDHFCDIEALDALGLRPIHHACRIGHTEIVKLLIDHYCDI 128          

HSP 8 Score: 98.2117 bits (243), Expect = 4.923e-25
Identity = 51/101 (50.50%), Postives = 69/101 (68.32%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLI 303
            LID+HC+I+AL ++ +RPIH+AC A H E V +LI  + +IE  D + WR IHHAC  G IE V LLI +HCDI ALD++   PI  A   G+ +I+ +LI
Sbjct:  287 LIDYHCDIEALDRWKRRPIHYACSADHKEAVKLLITHHCDIEAFDEWGWRPIHHACHLGSIETVNLLIDYHCDIGALDKSGNSPIEIAVRYGNEEIIHVLI 387          

HSP 9 Score: 58.151 bits (139), Expect = 4.911e-11
Identity = 41/121 (33.88%), Postives = 60/121 (49.59%), Query Frame = 1
Query:    1 LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILI----------------KEYYN---IEILDGFDWRLIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLLID 306
            LID+HC+I AL K    PI  A   G+ E +++LI                 ++ N   IE  D    R IH AC +G+IE VKLLI   C I   D++D  P+  A    + ++V+++ D
Sbjct:  353 LIDYHCDIGALDKSGNSPIEIAVRYGNEEIIHVLIGPCLLLACTNGDLEKVSKFLNGSTIEYCDKVQRRPIHWACESGNIELVKLLIKSKCKITIKDKSDLSPLDIARRNDNKELVQVIKD 473          
The following BLAST results are available for this feature:
BLAST of SMED30003476 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANKRD281.353e-1434.65ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:H... [more]
ANKRD281.353e-1434.65ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:H... [more]
ANKRD282.960e-1434.65ankyrin repeat domain 28 [Source:HGNC Symbol;Acc:H... [more]
ANKRD441.546e-1330.84ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:H... [more]
ANKRD441.674e-1330.84ankyrin repeat domain 44 [Source:HGNC Symbol;Acc:H... [more]
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BLAST of SMED30003476 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
trp-41.243e-1439.13TRP (Transient receptor potential) channel family ... [more]
unc-441.925e-928.28AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-441.925e-928.28AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-441.926e-928.28AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
unc-441.926e-928.28AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86][more]
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BLAST of SMED30003476 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nompC1.402e-1337.50gene:FBgn0016920 transcript:FBtr0300012[more]
nompC1.402e-1337.50gene:FBgn0016920 transcript:FBtr0304843[more]
nompC1.402e-1337.50gene:FBgn0016920 transcript:FBtr0343224[more]
nompC1.402e-1337.50gene:FBgn0016920 transcript:FBtr0347408[more]
nompC1.403e-1337.50gene:FBgn0016920 transcript:FBtr0347409[more]
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BLAST of SMED30003476 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ankrd28b6.883e-1532.71ankyrin repeat domain 28b [Source:ZFIN;Acc:ZDB-GEN... [more]
ankrd28b6.883e-1532.71ankyrin repeat domain 28b [Source:ZFIN;Acc:ZDB-GEN... [more]
ankrd164.983e-1230.93ankyrin repeat domain 16 [Source:ZFIN;Acc:ZDB-GENE... [more]
ankrd165.131e-1230.93ankyrin repeat domain 16 [Source:ZFIN;Acc:ZDB-GENE... [more]
ankrd52a1.410e-1132.08ankyrin repeat domain 52a [Source:NCBI gene;Acc:55... [more]
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BLAST of SMED30003476 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rev16.465e-1533.66REV1, DNA directed polymerase [Source:Xenbase;Acc:... [more]
rev17.263e-1533.66REV1, DNA directed polymerase [Source:Xenbase;Acc:... [more]
rev17.327e-1533.66REV1, DNA directed polymerase [Source:Xenbase;Acc:... [more]
rev17.398e-1533.66REV1, DNA directed polymerase [Source:Xenbase;Acc:... [more]
rev18.814e-1533.66REV1, DNA directed polymerase [Source:Xenbase;Acc:... [more]
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BLAST of SMED30003476 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Ankrd282.266e-1632.71ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MG... [more]
Ankrd288.360e-1534.65ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MG... [more]
Ankrd289.565e-1534.65ankyrin repeat domain 28 [Source:MGI Symbol;Acc:MG... [more]
Ankrd441.681e-1330.84ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MG... [more]
Ankrd441.763e-1330.84ankyrin repeat domain 44 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of SMED30003476 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|O15084|ANR28_HUMAN6.499e-1434.65Serine/threonine-protein phosphatase 6 regulatory ... [more]
sp|Q505D1|ANR28_MOUSE6.691e-1434.65Serine/threonine-protein phosphatase 6 regulatory ... [more]
sp|Q5UPG5|YL093_MIMIV5.042e-1331.53Putative ankyrin repeat protein L93 OS=Acanthamoeb... [more]
sp|Q8N8A2|ANR44_HUMAN8.040e-1330.84Serine/threonine-protein phosphatase 6 regulatory ... [more]
sp|B2RXR6|ANR44_MOUSE1.233e-1230.84Serine/threonine-protein phosphatase 6 regulatory ... [more]
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BLAST of SMED30003476 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A182GGY87.820e-2244.86ANK_REP_REGION domain-containing protein OS=Aedes ... [more]
A0A1X7T6P47.175e-2042.73Uncharacterized protein OS=Amphimedon queenslandic... [more]
A0A1X7T5X63.909e-1942.86Uncharacterized protein OS=Amphimedon queenslandic... [more]
A0A182H2I66.163e-1942.99Uncharacterized protein OS=Aedes albopictus OX=716... [more]
A0A1B8FHL11.484e-1833.64ANK_REP_REGION domain-containing protein OS=Pseudo... [more]
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BLAST of SMED30003476 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ANKRD527.612e-1233.71ankyrin repeat domain 52 [Source:HGNC Symbol;Acc:H... [more]
ankrd448.069e-1230.84ankyrin repeat domain 44 [Source:ZFIN;Acc:ZDB-GENE... [more]
ankrd449.038e-1230.84ankyrin repeat domain 44 [Source:ZFIN;Acc:ZDB-GENE... [more]
psmd102.305e-1132.99proteasome 26S subunit, non-ATPase 10 [Source:NCBI... [more]
psmd103.054e-1132.95proteasome 26S subunit, non-ATPase 10 [Source:NCBI... [more]
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BLAST of SMED30003476 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000003991.13.379e-1640.45pep scaffold:Pmarinus_7.0:GL484476:255315:281660:1... [more]
ENSPMAT00000007954.12.008e-1329.70pep scaffold:Pmarinus_7.0:GL479092:583:36301:1 gen... [more]
ENSPMAT00000010488.13.350e-1134.07pep scaffold:Pmarinus_7.0:GL501102:192066:206968:-... [more]
ankrd162.767e-1030.68ankyrin repeat domain 16 [Source:ZFIN;Acc:ZDB-GENE... [more]
ENSPMAT00000007332.14.256e-1032.18pep scaffold:Pmarinus_7.0:GL483392:1130:9476:1 gen... [more]
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BLAST of SMED30003476 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 3
Match NameE-valueIdentityDescription
GDE11.443e-727.91Glycerophosphocholine (GroPCho) phosphodiesterase;... [more]
PHO812.125e-628.40Cyclin-dependent kinase (CDK) inhibitor; regulates... [more]
MGA22.599e-633.33ER membrane protein involved in regulation of OLE1... [more]
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BLAST of SMED30003476 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO460991.469e-1231.53Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO341617.510e-1132.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO341622.531e-1032.05Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO347041.055e-930.77Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO421682.755e-929.63Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30003476 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ankrd28b1.559e-1535.64ankyrin repeat domain 28 [Source:NCBI gene;Acc:101... [more]
ankrd28b1.932e-1535.64ankyrin repeat domain 28 [Source:NCBI gene;Acc:101... [more]
ankrd28b2.742e-1535.64ankyrin repeat domain 28 [Source:NCBI gene;Acc:101... [more]
ENSORLT00000036692.11.299e-1433.66serine/threonine-protein phosphatase 6 regulatory ... [more]
ENSORLT00000018386.21.377e-1433.66serine/threonine-protein phosphatase 6 regulatory ... [more]
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BLAST of SMED30003476 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000032703.11.087e-6276.43SMESG000032703.1[more]
SMESG000010485.11.134e-3657.94SMESG000010485.1[more]
SMESG000010485.12.515e-3657.94SMESG000010485.1[more]
SMESG000017196.12.853e-3662.62SMESG000017196.1[more]
SMESG000017196.13.387e-3662.62SMESG000017196.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003476 ID=SMED30003476|Name=SMED30003476|organism=Schmidtea mediterranea sexual|type=transcript|length=321bp
CTGATTGATCACCATTGTAATATTGATGCACTTGGTAAATATGATCAACG
TCCAATTCATTTCGCGTGCATTGCAGGGCACATAGAAACCGTCAATATAC
TGATTAAAGAATATTATAATATCGAGATACTTGATGGGTTTGATTGGCGA
CTAATTCATCACGCATGCAGTGCAGGACACATAGAAGCAGTTAAACTGCT
GATTTATCACCATTGTGATATTGAAGCACTTGATCAGAATGATTGGCGGC
CAATTCATTACGCCTGCTGTGCAGGACACATGAAAATAGTCAAACTATTG
ATTGATTATCATTGTGATATT
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protein sequence of SMED30003476-orf-1

>SMED30003476-orf-1 ID=SMED30003476-orf-1|Name=SMED30003476-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=107bp
LIDHHCNIDALGKYDQRPIHFACIAGHIETVNILIKEYYNIEILDGFDWR
LIHHACSAGHIEAVKLLIYHHCDIEALDQNDWRPIHYACCAGHMKIVKLL
IDYHCDI
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000421tail
Vocabulary: INTERPRO
TermDefinition
IPR002110Ankyrin_rpt
IPR020683Ankyrin_rpt-contain_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005575cellular_component
GO:0005634nucleus
GO:0005654nucleoplasm
Vocabulary: biological process
TermDefinition
GO:0008150biological_process