Rapunzel 6

Overview
NameRapunzel 6
Smed IDSMED30003470
Length (bp)1458
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Rapunzel 6 (SMED30003470) t-SNE clustered cells

Violin plots show distribution of expression levels for Rapunzel 6 (SMED30003470) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003470

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30003470h1SMcG0021980 SmedASXL_018808SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30003470h1SMcG0021980 SmedASXL_018808SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
gutSMED30003470h1SMcG0021980 dd_Smed_v4_827_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30003470h1SMcG0021980 dd_Smed_v6_827_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30003470h1SMcG0021980 OX_Smed_1.0.03453ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Rapunzel 6 vs. Ensembl Zebrafish
Match: rpz6 (rapunzel 6 [Source:ZFIN;Acc:ZDB-GENE-170530-5])

HSP 1 Score: 60.8474 bits (146), Expect = 1.455e-9
Identity = 64/280 (22.86%), Postives = 127/280 (45.36%), Query Frame = 2 
Query:  158 ILDKAKEIFDNIKESDMYKTGKAI---AKWG-KPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVY-MLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGF--INQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETF--YINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLV 970
            ++ + KE  + + E  M++ G  +   A  G  PL EA     ++ +   ES  KE+T++K+QF ++  K+D +  Q E++   +    +  Q+   E ++    +  I I+   P   + +    FL  + +      +NLY  +  +  N  F E ++  + +Y   +R  +E F +   +L+  CL +     Y  L      E+    +WS ++  +   MK   EK  +++  Q+ +DI+    E  + + +  A++L +FL  KF    W + V
Sbjct:    8 VVAQKKEAIEEVME--MFERGAEVLASAVGGLSPLFEASAPVLRLFLDNVES--KEVTYVKDQFLVVRSKLDVLSSQIEDINCEIKKGRLDSQFFTVEENICNQFRKYIDILEAKP-EYKEVRKRLFLQHFPKT--GGEKNLYMLYDAVMGNSTFGEPILEVVEQYEARNRRVLEDFCVRLKELL--CLGIIALLGYCFLTQGEEAEQEKIQEWSTKIEEIETKMKETIEKCVQSFPEQAELDIKRLLKEKEEANLQETARELLDFLTKKFDWVCWSIRV 278
[Close]
BLAST of Rapunzel 6 vs. Ensembl Zebrafish
Match: rpz3 (rapunzel 3 [Source:ZFIN;Acc:ZDB-GENE-030131-4784])

HSP 1 Score: 59.6918 bits (143), Expect = 3.761e-9
Identity = 65/283 (22.97%), Postives = 126/283 (44.52%), Query Frame = 2 
Query:  155 NILDKAKEIFDNIKESDMYKTGKAI---AKWG-KPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVY-MLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAEN----LYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETF--YINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLV 970
             ++ + KE  + + E  M++ G  +   A  G  PL EA     K+++   ES  KE+T++K+QF ++  K+D +  Q +++   +    +  Q+   E ++    +  I I+   P   + +    FL   D  +    E     LY   +  N  F E ++  + +Y   +R  +E F +   +L+  CL +     Y  L      E+    +WS ++  +   MK   EK  +++  Q+ +DI+    E  + + +  A++L +FL  KF    W + V
Sbjct:    7 RVVAQKKEAIEAVME--MFEKGAEVLASAVGGLSPLFEAAAPVLKLVLNNVES--KEVTYVKDQFLVVRSKLDVLSCQLQDIDSEIRRRRLDTQFFSVEENMRNQFRKYIDILEAKP-EYKEVKKRLFL---DHFFITGGEKNLCLLYDAVMG-NSTFGEPILEVVEQYEARNRRVLEDFCVRLKELL--CLGIIALLGYCFLTQGEEAEQEKIQEWSTKIQEIETKMKETIEKCVQSFPEQAELDIKRLLKEKEEANLQETARELLDFLTKKFDWVCWSIRV 278
[Close]
BLAST of Rapunzel 6 vs. Ensembl Zebrafish
Match: rpz4 (rapunzel 4 [Source:ZFIN;Acc:ZDB-GENE-050417-472])

HSP 1 Score: 50.0618 bits (118), Expect = 4.308e-6
Identity = 60/278 (21.58%), Postives = 123/278 (44.24%), Query Frame = 2 
Query:  158 ILDKAKEIFDNIKESDMYKTG-KAIAKWGKPLLEAIGSFAKIIMVQFES-DSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVY-MLQHLIQIIADSPASLRHITIDKFLLSYDQV-YDNAAENLYQGFINQNKVFSENLVLAIR-KYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYD 976
            ++ + K++ +N+ E  +++ G + +A     L       A I+ +  ++ +SKE  +MKEQF+ + ++++ V ++ +++ + +  + V   Y   E ++    +  + ++   P   R +    FL  +++   D     LY      N  FS   VL I   Y +  R  +E++     +L    L     +  LK     EK  Q +WS++M  +   M  V E    ++  QS +D +    +   KS++  A  L   L  K+    W V +++
Sbjct:    8 LVAQKKDVVENVME--VFEQGAEVVASIAGDLFPVFSIAAPIVRLALDNVESKEAEYMKEQFQKVRDRLEVVSEEVQQINQEIKKSGVDATYFTVEENLTNQFRKFMDVLNAKP-KFREVRKKTFLDHFNRTGGDKNLHTLYSAVTGDN--FSGESVLEITLNYEQKSRRAVEEYCATLKKLFCVGLIALLGHAALKGDDEEEKLLQ-EWSEKMKVVQSKMIVVIEDCINSFPTQSELDAKRIVRDQSDKSNQQLADMLVEHLKKKYDWVCWSVRIFN 279
[Close]
BLAST of Rapunzel 6 vs. TrEMBL
Match: A0A0K1RMS6 (N-U9 (Fragment) OS=Pinctada fucata OX=50426 PE=4 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 8.100e-38
Identity = 88/330 (26.67%), Postives = 172/330 (52.12%), Query Frame = 2 
Query:  140 ILEKANILDKAKEIFDNIKESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSLPFGTRALNKNYYASICSRIP 1129
            IL  A I+ K  E  D   + D     K ++K   P  E IG    + MV     ++E+  +KE  + L+++   V  +F++V++  D   V+++ G  E  + +  + + ++   P    H   D F+++Y+  + N+A+ LY+  ++ N +F +NL       T+++RY +  F M  ++L+++ + VE  Y+++KN+T+  +F Q +W+ ++  ++  M  V + +   W  Q+ +D+E+F     + SH+++A  LYN    K+  R WLV+VYDP+  G  H + AC G  + +    N+ +SS+      +N+     I + +P
Sbjct:   40 ILRNATIVTKVVESLD---KGDFGSALKRLSKVTNPYTEVIG--PALTMVMHYKQNEEVKRLKELLQRLDQQFGKVHWEFKKVEKEFDELRVQVRLGAIEQKIRVTAYELALLHRIPREGVHHQADMFVMNYENDFQNSAKKLYEAVVDTNHIFHKNLFEYSVPLTENNRYVMHVFMMDVLRLLLQGINVEMSYLHIKNYTATYQFMQREWTLKIRKVLRTMDKVDKFVKSKWREQAEVDLEKFTKTKPELSHKDFAYSLYNTFKMKYPWRHWLVIVYDPITGGEKHWMSACGGFIKFRYYDRNIVISSVDESKPLVNETQAMDILANLP 364
[Close]
BLAST of Rapunzel 6 vs. TrEMBL
Match: A0A0A7NXM5 (Rapunzel (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 3.521e-37
Identity = 81/299 (27.09%), Postives = 158/299 (52.84%), Query Frame = 2 
Query:  176 EIFDNIKESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLK-NFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSL 1069
            ++F  ++E + +KT   I+    P+  AI +   ++ +  E +S E+  MK++F  LN ++D    +F+ +KR +DW+ V+I +   E ++  L  ++  + + P + R      F+ +Y+  Y  A + L++  +N  +++S NL+ A + Y ++ R Q+++F +G ++LI + +++E  Y+ LK N     KF Q  W  ++  L D ++ +       +  Q   D++   A +  +S+E +    Y+F   K+  R W VLVY  L    DHA+  C G  + + +G N+ VSS+
Sbjct:    9 QLFKFLEEKNFFKTLTQISSVAGPIFSAISAILTVVFMFIEIESPEMALMKKEFANLNNRIDKWGVEFQIIKRKIDWSAVQINFADIENNIITLHKMLSTLPEVPIANRETQAALFINTYEINYKAAGDILHRAMLNIEQIYSVNLLRAAQLYMENDRNQVQEFMVGMMKLITQAVQIEAAYVGLKYNNEEASKFTQSLWEKKLAVLKDKLEQIDTDTKNKYNEQYQKDLDNLLATHYSQSNERFVTIAYDFFATKYNWREWFVLVYKELSGFKDHAVVTCQGTHRFRTHGRNIIVSSV 307
[Close]
BLAST of Rapunzel 6 vs. TrEMBL
Match: V4B6X2 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_237783 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.123e-33
Identity = 99/376 (26.33%), Postives = 173/376 (46.01%), Query Frame = 2 
Query:   62 LLFLFVVIYISFDSVKSASTDINPVEILEKANILDKAKEIFDNIKESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQF-ESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKD--LYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSLPFGTRALNKNYYASICSRIPQNIGSWGHGPAHFVLLH 1180
            L  + +V+Y++  + K      + ++     N L  AKE+ D +   +       +A    P L  +G F   IM  F  S+S EL  +K  +  ++ + D VD QF EVKRL+DW+ ++IQY   E  +  +    + I          T   F+ +++  Y N+   LY G +N+ +VF + L  AI KY++  R + + F +G +QL++K  ++E  Y  LK  T+    +Q +W  R   +   M +    I   +  QS  DI+ +  ++ K +  N   +  LY FL  K+  R WL + Y+ +     H +  C G  + + +G NV V+S+      +N     S+ + +       G+ P    + H
Sbjct:    6 LAGVLLVLYVTVTTTKEIRDTASNIQ-----NGLSVAKELGDFVVAKNFTNVIGKLATAVTPFLGIVGPFVSFIMGFFGHSESAELKAIKRLYTEVDNRFDKVDLQFIEVKRLIDWSKIQIQYSDIEQKINAVNQEFENIYKFSIGAVKTTASAFVKNFESDYQNSGSKLYDGIMNEGRVFGDGLFTAIIKYSEYDRGKSQTFMLGLLQLLMKAAKLELTYHQLKGHTAALHDYQNQWRTRFTHIRIKMMSTDNSIVSQYHTQSGKDIDNYLIKHPKNTMNNNDFNNMLYGFLTKKYSWRDWLTITYNDITGSNVHYVGECGGYLKFRNHGRNVVVASVDRRKGHINMAQAQSVVNNVDSCHPRTGYRPHKHTVYH 376
[Close]
BLAST of Rapunzel 6 vs. TrEMBL
Match: A0A267FYJ9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig026676g3 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 2.377e-32
Identity = 79/290 (27.24%), Postives = 143/290 (49.31%), Query Frame = 2 
Query:  299 SDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQ-RNGHNVAV-SSLPFGTRALNKNYYASICSRIPQNIGSWGHGPA 1162
             +S E+ F++EQF  ++ K+D +  Q ++VKRL++W   ++ Y  YE  +     ++     +P S R +   +F+ ++ Q    A   LYQ  + +N V +ENL  A+  YT++HR  +  F +   +LI++  ++E  Y  + +        +  W  RM  L+  MK +   ++  + +Q   DI     +N   S+++++  +Y FL DKF  R W+ L Y  +  G  H +  C G ++ +   G NV V SS P  +R       A++ +   Q +GS  H  +
Sbjct:    6 GESAEMKFLREQFAQVHAKLDSISLQLDDVKRLIEWRTTRVMYANYETFIIAASSILNNYVAAPESSREVLKRQFIAAF-QHNPLAPLKLYQAIVEKNFVIAENLAAAVVHYTQNHRRHVINFLLAMQRLIMRGAQLEIAYEKMVHGDGAGNVAKTVWDGRMQKLLSRMKQLETDVTNRYHSQMTSDIGRVILDNRHLSNKDFSGHMYRFLADKFDRRYWIALSYKDVSGGDKHWVSYCGGDARFRTHGGRNVVVASSNPGSSRFTRAKGNAALDA--AQRVGSGSHAES 292
[Close]
BLAST of Rapunzel 6 vs. TrEMBL
Match: V4AXV0 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_238695 PE=4 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 7.776e-32
Identity = 91/341 (26.69%), Postives = 163/341 (47.80%), Query Frame = 2 
Query:   56 KALLFLFVVIYISFDSVKSASTDINPVEILEKANILDKAKEIFDNIKESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQF-ESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKD--LYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSL 1069
            + L  + +V+Y+S  + K      + ++     N L  AKE+ D +           +A    P L  +G F   IM  F +S+S EL  +K  +  ++ + D VD QF EVKR++DW+ +++QY   E  +  +    + I     +    T   F+ +Y+  Y N+   LY G +N+ +VF + L  A+ KY++  R + + F +G +QL++K  ++E  Y  LK   +    +Q +W  R   +   M +    I   +  QS  DI+ +  ++ K +  N   +  LY FL+ K+  R W  + Y+ +     H +  C G  + + +G NV V+S+
Sbjct:    4 EVLAGVLLVLYVSVTNTKEIRDTASNIQ-----NGLSLAKELGDFVVAKKFTNVIGKLATAVTPFLGIVGPFVSFIMGFFGQSESAELKAIKRLYTEVDNRFDKVDLQFIEVKRMIDWSKIQVQYSDIEQKINAVNKAFENIYKFSTAAVKTTTSAFVSNYESDYQNSGSKLYDGIMNEGRVFGDGLFTAVVKYSEYDRVKSQTFMLGLLQLLMKAAKLELTYHQLKGHKAALHDYQNQWRTRFTHIRIKMMSTDSSIVSQYHTQSGKDIDNYLIKHQKNTMNNNDFNNALYAFLSKKYFWRDWFTVTYNDITGSNVHYVGECGGHIKFRNHGRNVVVASV 339
[Close]
BLAST of Rapunzel 6 vs. Ensembl Cavefish
Match: rpz6 (uncharacterized LOC103039477 [Source:NCBI gene;Acc:103039477])

HSP 1 Score: 52.373 bits (124), Expect = 5.663e-7
Identity = 60/286 (20.98%), Postives = 121/286 (42.31%), Query Frame = 2 
Query:  128 NPVE--ILEKANILDKAKEIFDNIKESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVY-MLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGF--INQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLV 970
            NP+E  + +K   ++   E F+   E      G+       PL E      ++ +   +S  KE+ ++KEQF  +  K+D +  Q E++   +    +  QY   E ++    +  + I+   P   R +    FL  + +      +NLY  +  +  +  F  +++  + +Y   +R  +E F +   +L    L     +  L     +E+    +WS+++  +   MK   E+    +  Q+ +D++    E   KS ++ A++L  FL  K+    W V V
Sbjct:    3 NPLEKVVAQKKEAIEAVMETFERGSEVLASAVGELF-----PLCEVAAPVVRLALDNVQS--KEVVYVKEQFLAVRNKLDVLSNQLEDIDCEIKKGRLDYQYFSVEENIRNQFRKYLDILEAKP-QFREVKKRLFLEHFSKT--GGEKNLYDLYDALMGSSTFGGSVLEVVEEYEARNRRVLEDFCVRMKELYCLGLIALLGHCALTQGEDVEQEKIQEWSEKIQEVETKMKASIEECVAFFAEQAKLDVQRLIQEKEDKSLQDIAQELLTFLVRKYDWVSWSVRV 278
[Close]
BLAST of Rapunzel 6 vs. Planmine SMEST
Match: SMESG000004912.1 (SMESG000004912.1)

HSP 1 Score: 902.894 bits (2332), Expect = 0.000e+0
Identity = 435/435 (100.00%), Postives = 435/435 (100.00%), Query Frame = 2 
Query:   47 MITKALLFLFVVIYISFDSVKSASTDINPVEILEKANILDKAKEIFDNIKESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSLPFGTRALNKNYYASICSRIPQNIGSWGHGPAHFVLLHYLQPKMECGINQIGAVIRSKSTKVYLAPADRMVSCRWAGYYNRFVDIHKTLDLIILG 1351
            MITKALLFLFVVIYISFDSVKSASTDINPVEILEKANILDKAKEIFDNIKESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSLPFGTRALNKNYYASICSRIPQNIGSWGHGPAHFVLLHYLQPKMECGINQIGAVIRSKSTKVYLAPADRMVSCRWAGYYNRFVDIHKTLDLIILG
Sbjct:    1 MITKALLFLFVVIYISFDSVKSASTDINPVEILEKANILDKAKEIFDNIKESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSLPFGTRALNKNYYASICSRIPQNIGSWGHGPAHFVLLHYLQPKMECGINQIGAVIRSKSTKVYLAPADRMVSCRWAGYYNRFVDIHKTLDLIILG 435
[Close]
BLAST of Rapunzel 6 vs. Planmine SMEST
Match: SMESG000062792.1 (SMESG000062792.1)

HSP 1 Score: 348.977 bits (894), Expect = 3.456e-116
Identity = 167/360 (46.39%), Postives = 249/360 (69.17%), Query Frame = 2 
Query:  122 DINPVEILEKANILDKAKEIFDNIKESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSLPFGTRALNKNYYASICSRIPQNIGSWGHGPAHFVLLHYLQPKME 1201
            D +P +ILE+A+ L+KAKEIF++IKESD  KT +AI+KWG PLL++IG F+K+IM QFE++SKELT+MKEQFKI+N+KMD VDQQF+EVK+ ++WNVVK+QY  +E  + +LQ +I  I+D+P+++R + +D+F ++YDQ Y NAA+ LY+ F N  ++FSENLV A  +    HR ++EKFF+GNIQLI+K LEVE+FY+  KN TS + +W  +W+ ++N +  IM+     I  NW +Q   D EEFN +N  K ++ Y ++ YNFL DKF  R W ++VY+P+  G +H +    GI  ++  GHN+AV+S        N N  + +   +P++  + G G A  +L+  L P ++
Sbjct:   22 DTSPGKILEEASKLEKAKEIFNSIKESDFVKTTQAISKWGLPLLKSIGYFSKVIMAQFETESKELTYMKEQFKIINDKMDTVDQQFDEVKKAINWNVVKVQYFNHETAIGLLQDMILSISDAPSNVRQMMMDRFRITYDQTYYNAADKLYESFTNPQRIFSENLVTASCQTFNFHRRKVEKFFLGNIQLILKGLEVESFYLTTKNLTSNQDYWAEQWNKKINQISAIMEETINSIKGNWHSQLQKDAEEFNVKNYGKGNKEYLEEFYNFLTDKFSWREWFLVVYNPITGGDNHYVSVYGGIINLRSRGHNIAVASRDKEDNNFNANLISQVLDNLPRSFNTRGKGLAESLLMDQLIPAIQ 381
[Close]
BLAST of Rapunzel 6 vs. Planmine SMEST
Match: SMESG000052592.1 (SMESG000052592.1)

HSP 1 Score: 154.451 bits (389), Expect = 1.463e-41
Identity = 86/332 (25.90%), Postives = 173/332 (52.11%), Query Frame = 2 
Query:   77 VVIYISFDSVKSASTDINPVEILEKANILDKAKEIFDNIKESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLK-NFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSL 1069
            +++Y     +K++ T+    +I+   N +    ++F  ++E + +KT   I+    P+  AI +   ++ +  E +S E+  MK++F  LN ++D    +F+ +KR +DW+ V+I +   E ++  L  ++  + + P + R      F+ +Y+  Y  A + L++  +N  +++S NL+ A + Y ++ R Q+++F +G ++LI + +++E  Y+ LK N     KF Q  W  ++  L D ++ +       +  Q   D++   A +  +S+E +    Y+F   K+  R W VLVY  L    DHA+  C G  + + +G N+ VSS+
Sbjct:   27 LILYRGLPQIKTSLTEKEKSQIM---NGVQGVVQLFKFLEEKNFFKTLTQISSVAGPIFSAISAILTVVFMFIEIESPEMALMKKEFANLNNRIDKWGVEFQIIKRKIDWSAVQINFADIENNIITLHKMLSTLPEVPIANRETQAALFINTYEINYKAAGDILHRAMLNIEQIYSVNLLRAAQLYMENDRNQVQEFMVGMMKLITQAVQIEAAYVGLKYNNEEASKFTQSLWEKKLAVLKDKLEQIDTDTKNKYNEQYQKDLDNLLATHYSQSNERFVTIAYDFFATKYNWREWFVLVYKELSGFKDHAVVTCQGTHRFRTHGRNIIVSSV 355
[Close]
BLAST of Rapunzel 6 vs. Planmine SMEST
Match: SMESG000052591.1 (SMESG000052591.1)

HSP 1 Score: 120.553 bits (301), Expect = 1.297e-29
Identity = 81/293 (27.65%), Postives = 138/293 (47.10%), Query Frame = 2 
Query:  197 ESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIE--KFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSL 1069
            E D  K GK +A           S      +  E  + E    K+   +L  +     + F EV+  +DW VV+I +   E  +  +Q L         + +  +  +FL SY+  Y    + LY   IN N+ FS NL  A +K+T+  R +++ F +   +L+++ + +E  + NLK + + E  KF    W +R  +L   M+ V     E +  Q  MD++E+       ++E + + LYN+L+ K+  R W V+VY P+    DH +  C G  + + NG N+ VSS+
Sbjct:   42 EKDFNKVGKLMADITDETFNVSSSTIFTAFLSGELHATETESFKKASLLLKTRTSRWKEDFNEVRYSIDWAVVQINFEDLENRILTVQRLSTEFMSIDDNEKEFSAKQFLHSYEANYKATGDLLYSVMININQSFSGNLFRASQKFTQFDRKKVQTFMVELAKLLVQAVNIEANFANLK-YENDENVKFLNSMWRERFKTLKATMEAVDLDTKEQFRNQYQMDLDEYLMTRESLTNEQFNQVLYNYLSTKYNWRDWFVVVYAPISGFKDHTVSTCQGTYRFRTNGRNIIVSSV 333
[Close]
BLAST of Rapunzel 6 vs. Planmine SMEST
Match: SMESG000027656.1 (SMESG000027656.1)

HSP 1 Score: 83.1889 bits (204), Expect = 7.803e-17
Identity = 82/360 (22.78%), Postives = 153/360 (42.50%), Query Frame = 2 
Query:   62 LLFLFVVIYISFDSVKSASTDINPVEILEKANILDKAKEIFDNIKE-SDMYKTGKAIAKWGK------PLLEAIGSFAKII-MVQFESDSKELTFMKEQFKILNEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPASLRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDHRYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLMDIMKTVSEKISENWEAQSNMDIEEFNAE---NGKKSHENYAKDLYNFLNDKFRNRIWLVLVYDPLDSGTDHALKACNGISQM--QRNGHNVAVSSLPFGTRALNKNY 1102
            ++FLF+    S  SV   S + + ++  +   +L  +K++F N+ E       GK  AK         P   AI +   I+ M   + DS EL  +KE F+ +N K+D +  +   ++ +++ +  +  Y K    V  + +  + + ++  S      D F   Y+  ++ A  N+Y         F  N+V   + + + +  ++ +F    +QLI K L VE  Y+   NF++  K     W+ ++N +      V E    NW+  +    +E + E   + +   E  AKDL N        R WL + Y+       H       +  +  +RN H+ +V S+P   +    +Y
Sbjct:    5 IVFLFIACLFSLFSVIKCSYEADTIKKTQA--VLASSKDVFANLSEFITSANPGKLFAKLSGVLGSLGPAAMAISAILSIVTMFLPKEDSPELKAIKEGFEKVNNKLDTISSKIGSLENIIELSSARAAYVK---TVTSINNNYETLNEAKKSNSKEGYDYFAKQYENSFEFATANMYDAMTGSTGPFGFNMVNLYKTHVRCNPVKLSQFCTMGLQLITKGLTVEGAYLGYMNFSAKAKGKTETWTTKINEVSKQCSAVIEACESNWKEFAYQQYKETHRELEGDNQAFAERLAKDLENVA----PQRSWLTIAYNDASGFGPHTYSTYYEVHTIWNERN-HDTSVFSVPKSPKLTGSDY 354
The following BLAST results are available for this feature:
BLAST of Rapunzel 6 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel 6 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel 6 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel 6 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
rpz61.455e-922.86rapunzel 6 [Source:ZFIN;Acc:ZDB-GENE-170530-5][more]
rpz33.761e-922.97rapunzel 3 [Source:ZFIN;Acc:ZDB-GENE-030131-4784][more]
rpz44.308e-621.58rapunzel 4 [Source:ZFIN;Acc:ZDB-GENE-050417-472][more]
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BLAST of Rapunzel 6 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel 6 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel 6 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel 6 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K1RMS68.100e-3826.67N-U9 (Fragment) OS=Pinctada fucata OX=50426 PE=4 S... [more]
A0A0A7NXM53.521e-3727.09Rapunzel (Fragment) OS=Schmidtea mediterranea OX=7... [more]
V4B6X21.123e-3326.33Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A267FYJ92.377e-3227.24Uncharacterized protein OS=Macrostomum lignano OX=... [more]
V4AXV07.776e-3226.69Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
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BLAST of Rapunzel 6 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rpz65.663e-720.98uncharacterized LOC103039477 [Source:NCBI gene;Acc... [more]
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BLAST of Rapunzel 6 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel 6 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel 6 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel 6 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Rapunzel 6 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000004912.10.000e+0100.00SMESG000004912.1[more]
SMESG000062792.13.456e-11646.39SMESG000062792.1[more]
SMESG000052592.11.463e-4125.90SMESG000052592.1[more]
SMESG000052591.11.297e-2927.65SMESG000052591.1[more]
SMESG000027656.17.803e-1722.78SMESG000027656.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003470 ID=SMED30003470|Name=Rapunzel 6|organism=Schmidtea mediterranea sexual|type=transcript|length=1458bp
AATATTTAAAATGCGTTGATATTTGTTTTAATCATATTTTTTTCCGATGA
TTACAAAAGCTCTCCTATTTCTGTTTGTCGTCATTTACATTTCATTTGAC
TCAGTAAAGTCAGCCTCAACTGACATAAATCCAGTTGAGATTTTAGAAAA
GGCAAACATTTTAGACAAAGCTAAGGAAATATTTGATAACATAAAAGAAA
GTGATATGTACAAAACTGGAAAAGCAATAGCTAAATGGGGCAAACCGTTG
TTAGAAGCGATTGGAAGTTTTGCCAAAATCATCATGGTACAATTTGAATC
CGACTCCAAAGAGTTGACATTCATGAAAGAGCAATTTAAAATATTAAATG
AAAAAATGGATTATGTCGACCAACAATTTGAAGAAGTCAAACGACTTATG
GACTGGAATGTCGTCAAAATCCAATACGGAAAATATGAAATGCATGTGTA
TATGCTTCAACATTTAATTCAGATAATTGCAGACTCTCCTGCCTCTCTGA
GACATATTACTATTGACAAGTTCCTACTTTCTTACGATCAAGTCTATGAT
AATGCTGCAGAAAACCTTTACCAAGGATTTATAAATCAAAATAAAGTGTT
TTCAGAAAATTTAGTTTTAGCGATCAGAAAGTACACCAAAGATCATCGGT
ATCAAATTGAAAAGTTTTTCATGGGCAACATTCAGTTGATTATCAAATGT
TTGGAAGTGGAAACATTTTACATAAATTTGAAAAATTTTACAAGCATTGA
AAAATTTTGGCAAGTAAAATGGAGTGATCGAATGAACAGTCTAATGGATA
TCATGAAGACTGTATCAGAAAAAATTAGTGAGAATTGGGAAGCCCAATCA
AATATGGACATAGAAGAATTTAATGCGGAAAATGGTAAAAAATCTCACGA
AAATTACGCTAAAGATTTGTACAATTTCCTCAATGATAAATTCAGAAATA
GAATTTGGTTGGTTTTGGTTTATGATCCACTAGACTCGGGCACAGATCAC
GCACTAAAGGCTTGCAATGGAATTAGCCAAATGCAGCGTAATGGACACAA
TGTTGCGGTGAGCAGTCTACCTTTTGGCACAAGAGCACTCAATAAGAATT
ATTATGCAAGTATTTGTAGTCGAATCCCACAGAACATTGGCAGCTGGGGT
CATGGACCAGCTCATTTTGTTTTACTGCATTATTTACAACCAAAAATGGA
ATGTGGTATAAATCAAATAGGAGCAGTAATTCGATCAAAATCAACTAAAG
TGTATCTGGCTCCAGCCGATCGTATGGTAAGTTGTAGATGGGCGGGCTAT
TACAATAGATTTGTAGATATACACAAGACGCTTGATTTGATCATTTTAGG
TTGATGCAAGGAAAATGTATTTTTTAAAAGACGCATTCAAAAATTGTATT
TTGGCTTCGTAAATTTAAGAAACTCTGTAAAATAAATTGAGTATTTGTTA
AAAAAAAA
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protein sequence of SMED30003470-orf-1

>SMED30003470-orf-1 ID=SMED30003470-orf-1|Name=SMED30003470-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=436bp
MITKALLFLFVVIYISFDSVKSASTDINPVEILEKANILDKAKEIFDNIK
ESDMYKTGKAIAKWGKPLLEAIGSFAKIIMVQFESDSKELTFMKEQFKIL
NEKMDYVDQQFEEVKRLMDWNVVKIQYGKYEMHVYMLQHLIQIIADSPAS
LRHITIDKFLLSYDQVYDNAAENLYQGFINQNKVFSENLVLAIRKYTKDH
RYQIEKFFMGNIQLIIKCLEVETFYINLKNFTSIEKFWQVKWSDRMNSLM
DIMKTVSEKISENWEAQSNMDIEEFNAENGKKSHENYAKDLYNFLNDKFR
NRIWLVLVYDPLDSGTDHALKACNGISQMQRNGHNVAVSSLPFGTRALNK
NYYASICSRIPQNIGSWGHGPAHFVLLHYLQPKMECGINQIGAVIRSKST
KVYLAPADRMVSCRWAGYYNRFVDIHKTLDLIILG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR039051SE-CTX-like
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039051SE-cephalotoxin-likePANTHERPTHR40472FAMILY NOT NAMEDcoord: 58..327
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..22
score: 0.815