Glutamate receptor, ionotropic kainate

Overview
NameGlutamate receptor, ionotropic kainate
Smed IDSMED30003453
Length (bp)4661
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Glutamate receptor, ionotropic kainate (SMED30003453) t-SNE clustered cells

Violin plots show distribution of expression levels for Glutamate receptor, ionotropic kainate (SMED30003453) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Glutamate receptor, ionotropic kainate (SMED30003453) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Glutamate receptor, ionotropic kainate (SMED30003453) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003453

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 25

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
anterior sideSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30003453h1SMcG0004827 dd_Smed_v4_12299_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003453h1SMcG0004827 dd_Smed_v4_12299_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30003453h1SMcG0004828 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30003453h1SMcG0004827 dd_Smed_v4_4267_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30003453h1SMcG0004827 dd_Smed_v4_12299_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Human
Match: GRIK4 (glutamate ionotropic receptor kainate type subunit 4 [Source:HGNC Symbol;Acc:HGNC:4582])

HSP 1 Score: 212.231 bits (539), Expect = 2.105e-55
Identity = 105/194 (54.12%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMDVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 799          

HSP 2 Score: 143.665 bits (361), Expect = 6.879e-34
Identity = 129/503 (25.65%), Postives = 223/503 (44.33%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSCRFPESLDRYPMD----IQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAI----LFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            S   L I  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G+VA++ P +   S  I  ++C +  +PH   +   PE   ++       + L P+    +  +   +          LI +   C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    ++          L+ A+   M    +++IFT+ + SL  + +  ++++VNI  FSI + S                +   +    S + + +    + PA+    LFD V  +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E L G+TG I FN   QR NY L I++ T NGF++ G W+    + +   +  S +        L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI ++
Sbjct:   21 SPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVA---PEEFVKFQFQRFTTLNLHPSNTDISVAVAGILNFFNC-TTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEI-RDDKTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSL-VDDRVNILGFSIFNQS------------HAFFQEFAQSLNQSWQENCDHVPFTGPALSSALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALKILQFTRNGFRQIGQWHVAEGLSMDSHLYASNISDTLFNTTLVVTTILENPYLMLKGNHQEMEGNDR----YEGFCVDMLKELAEILRFNYKIRLVGDGVYGV-------PEANGTWTGMVGELIARK 493          

HSP 3 Score: 123.25 bits (308), Expect = 1.705e-27
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I  +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  489 LIARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Human
Match: GRIK4 (glutamate ionotropic receptor kainate type subunit 4 [Source:HGNC Symbol;Acc:HGNC:4582])

HSP 1 Score: 212.231 bits (539), Expect = 2.105e-55
Identity = 105/194 (54.12%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMDVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 799          

HSP 2 Score: 143.665 bits (361), Expect = 6.879e-34
Identity = 129/503 (25.65%), Postives = 223/503 (44.33%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSCRFPESLDRYPMD----IQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAI----LFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            S   L I  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G+VA++ P +   S  I  ++C +  +PH   +   PE   ++       + L P+    +  +   +          LI +   C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    ++          L+ A+   M    +++IFT+ + SL  + +  ++++VNI  FSI + S                +   +    S + + +    + PA+    LFD V  +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E L G+TG I FN   QR NY L I++ T NGF++ G W+    + +   +  S +        L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI ++
Sbjct:   21 SPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVA---PEEFVKFQFQRFTTLNLHPSNTDISVAVAGILNFFNC-TTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEI-RDDKTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSL-VDDRVNILGFSIFNQS------------HAFFQEFAQSLNQSWQENCDHVPFTGPALSSALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALKILQFTRNGFRQIGQWHVAEGLSMDSHLYASNISDTLFNTTLVVTTILENPYLMLKGNHQEMEGNDR----YEGFCVDMLKELAEILRFNYKIRLVGDGVYGV-------PEANGTWTGMVGELIARK 493          

HSP 3 Score: 123.25 bits (308), Expect = 1.705e-27
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I  +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  489 LIARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Human
Match: GRIK4 (glutamate ionotropic receptor kainate type subunit 4 [Source:HGNC Symbol;Acc:HGNC:4582])

HSP 1 Score: 212.231 bits (539), Expect = 2.105e-55
Identity = 105/194 (54.12%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMDVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 799          

HSP 2 Score: 143.665 bits (361), Expect = 6.879e-34
Identity = 129/503 (25.65%), Postives = 223/503 (44.33%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSCRFPESLDRYPMD----IQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAI----LFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            S   L I  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G+VA++ P +   S  I  ++C +  +PH   +   PE   ++       + L P+    +  +   +          LI +   C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    ++          L+ A+   M    +++IFT+ + SL  + +  ++++VNI  FSI + S                +   +    S + + +    + PA+    LFD V  +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E L G+TG I FN   QR NY L I++ T NGF++ G W+    + +   +  S +        L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI ++
Sbjct:   21 SPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVA---PEEFVKFQFQRFTTLNLHPSNTDISVAVAGILNFFNC-TTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEI-RDDKTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSL-VDDRVNILGFSIFNQS------------HAFFQEFAQSLNQSWQENCDHVPFTGPALSSALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALKILQFTRNGFRQIGQWHVAEGLSMDSHLYASNISDTLFNTTLVVTTILENPYLMLKGNHQEMEGNDR----YEGFCVDMLKELAEILRFNYKIRLVGDGVYGV-------PEANGTWTGMVGELIARK 493          

HSP 3 Score: 123.25 bits (308), Expect = 1.705e-27
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I  +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  489 LIARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Human
Match: GRIK5 (glutamate ionotropic receptor kainate type subunit 5 [Source:HGNC Symbol;Acc:HGNC:4583])

HSP 1 Score: 211.46 bits (537), Expect = 3.710e-55
Identity = 101/194 (52.06%), Postives = 141/194 (72.68%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMK-KNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +P++S +DLA QTNI+YGT+  G++ TFF+ +   TYQ+M+ +M+ K   VFV STE+G+ +V NS  +YAFLLES MN+++ + +C+L +IG LLD+KGYGIG P GSP RD +T +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  607 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMWNYMQSKQPSVFVKSTEEGIARVLNS--RYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQENNRLEILKRKWWEGGRCPKEEDHRAKG 798          

HSP 2 Score: 122.865 bits (307), Expect = 1.951e-27
Identity = 58/120 (48.33%), Postives = 81/120 (67.50%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSE--LDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC       L+NQ+ L NSLWF VG  ++QG
Sbjct:  488 LINRKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQG 607          

HSP 3 Score: 119.783 bits (299), Expect = 1.809e-26
Identity = 111/438 (25.34%), Postives = 197/438 (44.98%), Query Frame = 1
Query:  313 CNLIRHGLVAMISPIAEVPSRH-ISSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            C ++  G+V+++ P +   S   +S +C +  IPH+      PE   R        + L P+ +  +  + + +     +    LI +   C+L+ E L+  F  S+  +   +  D +    LL+E+ +  K    ++D         L  AS   MT   + +I T  D  ++ +    + +  NI  FS+  T H       ++     +        E       +  A  +  A++FD V+ +   +  L +S+ + V+P+ C S + W  G+SL+N +   E   G+TG++ FN   QR NY L I+E +  G ++ G W  +R + +        +      + L VTTI+  P+V  +   P+ Q    N E + GFC+D+LRE+   L   + + LV+D  YG        P+GS  W GM+GELI ++
Sbjct:   83 CQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVG---PEETPRLQYLRFASVSLYPSNEDVSLAVSRILKSFN-YPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEI-RDDKVSTIIIDANASISHLILRKASELGMTSAFYKYILTTMDFPILHLDGI-VEDSSNILGFSMFNTSHPFYPEFVRSLNMSWR--------ENCEASTYLGPALSA--ALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLANKTLVVTTILENPYVMRR---PNFQALSGN-ERFEGFCVDMLRELAELLRFRYRLRLVEDGLYG-----APEPNGS--WTGMVGELINRK 492          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Human
Match: GRIK5 (glutamate ionotropic receptor kainate type subunit 5 [Source:HGNC Symbol;Acc:HGNC:4583])

HSP 1 Score: 211.46 bits (537), Expect = 3.710e-55
Identity = 101/194 (52.06%), Postives = 141/194 (72.68%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMK-KNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +P++S +DLA QTNI+YGT+  G++ TFF+ +   TYQ+M+ +M+ K   VFV STE+G+ +V NS  +YAFLLES MN+++ + +C+L +IG LLD+KGYGIG P GSP RD +T +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  607 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMWNYMQSKQPSVFVKSTEEGIARVLNS--RYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQENNRLEILKRKWWEGGRCPKEEDHRAKG 798          

HSP 2 Score: 122.865 bits (307), Expect = 1.951e-27
Identity = 58/120 (48.33%), Postives = 81/120 (67.50%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSE--LDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC       L+NQ+ L NSLWF VG  ++QG
Sbjct:  488 LINRKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQG 607          

HSP 3 Score: 119.783 bits (299), Expect = 1.809e-26
Identity = 111/438 (25.34%), Postives = 197/438 (44.98%), Query Frame = 1
Query:  313 CNLIRHGLVAMISPIAEVPSRH-ISSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            C ++  G+V+++ P +   S   +S +C +  IPH+      PE   R        + L P+ +  +  + + +     +    LI +   C+L+ E L+  F  S+  +   +  D +    LL+E+ +  K    ++D         L  AS   MT   + +I T  D  ++ +    + +  NI  FS+  T H       ++     +        E       +  A  +  A++FD V+ +   +  L +S+ + V+P+ C S + W  G+SL+N +   E   G+TG++ FN   QR NY L I+E +  G ++ G W  +R + +        +      + L VTTI+  P+V  +   P+ Q    N E + GFC+D+LRE+   L   + + LV+D  YG        P+GS  W GM+GELI ++
Sbjct:   83 CQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVG---PEETPRLQYLRFASVSLYPSNEDVSLAVSRILKSFN-YPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEI-RDDKVSTIIIDANASISHLILRKASELGMTSAFYKYILTTMDFPILHLDGI-VEDSSNILGFSMFNTSHPFYPEFVRSLNMSWR--------ENCEASTYLGPALSA--ALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLANKTLVVTTILENPYVMRR---PNFQALSGN-ERFEGFCVDMLRELAELLRFRYRLRLVEDGLYG-----APEPNGS--WTGMVGELINRK 492          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Celegans
Match: glr-3 (GLutamate Receptor family (AMPA) [Source:UniProtKB/TrEMBL;Acc:Q21415])

HSP 1 Score: 199.519 bits (506), Expect = 3.366e-52
Identity = 87/184 (47.28%), Postives = 137/184 (74.46%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNIT 4003
            G E CP+A+STR+++G WWFF LI+IS+YTANLAA LT  ++  PI++ +DLA QT IKYGTL  G++ +FF ++ I TY++M++ M  +  +FV S+++G+ +V++S+  YA+L+ES+M ++  +RDC LM+IG L+D KGYGIG P GSP R+ ++ +IL+LQ+  ++  L  KWW + ++ 
Sbjct:  585 GSELCPRAASTRLLTGIWWFFALILISSYTANLAAVLTTRRMETPIENADDLAAQTKIKYGTLGRGSTMSFFNESKIETYERMWQLMSSSPGLFVQSSKEGIARVKSSD--YAYLMESSMLEYAVERDCELMQIGGLIDQKGYGIGLPKGSPYRELISTAILRLQEKTELTELKEKWWKDKSVV 766          

HSP 2 Score: 107.071 bits (266), Expect = 4.567e-23
Identity = 54/117 (46.15%), Postives = 78/117 (66.67%), Query Frame = 3
Query: 1932 QAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSE---LDNQFNLLNSLWFTVGALVRQG 2273
             A+LA+A +TISY R +V+DFT P+M LG+SILF KP+      F F+ P+S  VW+   ++Y  VS  I+ I+ ++PYE  Q    +DN +   +DNQF+L NS WFTV +L++QG
Sbjct:  471 DADLAVASLTISYGRSEVIDFTVPYMHLGISILFKKPRIRDSDWFKFMDPLSTQVWIMTFASYFVVSVAIWIIAKISPYE--QFERDEDNGQYKPVDNQFSLRNSFWFTVCSLMQQG 585          

HSP 3 Score: 90.8929 bits (224), Expect = 4.925e-18
Identity = 116/443 (26.19%), Postives = 191/443 (43.12%), Query Frame = 1
Query:  313 CNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFP-ESLDRYPM---DIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLL--NQFESRFKMRQWVHHDLKKNKKLLRELG--KSKKYRY-FLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPS-VVEIGNIV-------RPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGEL 1590
            CN +  G  A+I     +P ++   L +   +  +  +   P  SL   P       L P+ +  +  +  +I + E W+D++++    + +L+   ++    F+      Q V   LK       EL   K+K  RY  +++IP  +   FL  A+  +M    + ++  D D   VDI + +     NIT+F + D           E +E + + IV   K+S +   +K      +I  DT+  L    IR +KS  +   P +C S  +W+ GS  I        L GI+G + +  + +R NY L +   T + FQK   W+        +RI  S  V I N         + LK++  +  PFV             T+  ++ G+CIDLL +I N L   +TI  V+DN YG    N        KW+GM+GEL
Sbjct:   76 CNCLILGASAII-----LPEQYDGHLAAAAIVQSIADNTNVPCVSLHLSPPTRPSTHLHPHLNAKSLAVAAFIKR-EKWKDVVVVFEEPDELLEITDMITAGHFDPDSFSSQLVR--LKHGDDYGNELKHIKNKLDRYRIVINIPLQKALHFLEQAANMSMCGVLYHYVVMDMDLVTVDIDSIRGIEDCNITSFGVHD--------VNSEYIEDIRQEIVH--KSSIRLP-KKGVPYTTSIWIDTLRLL----IRSMKSIQIWDEP-RCGS--SWKSGSD-IKKRFFENPLAGISGDLHWAPSGERSNYTLHVYRRTLS-FQKFAEWSSRT-----RRIASSEAVVIANSSEKLTLEGKHLKISVYLEAPFVMI-----------TSNGSYEGYCIDLLHKIANILKFTYTIQKVRDNAYGSKESNG-------KWSGMVGEL 467          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Celegans
Match: glr-4 (GLutamate Receptor family (AMPA) [Source:UniProtKB/TrEMBL;Acc:Q17697])

HSP 1 Score: 192.2 bits (487), Expect = 1.213e-49
Identity = 90/199 (45.23%), Postives = 137/199 (68.84%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNRE-VFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTE 4045
            G +  P+A++TR+++  WW FT IIIS+YTA LAAFLTVE++  PI+S +DLA Q  I+YG LK G++  FF ++ IP Y++M+  M+ +   VFV+S+ +G+ +V+  +G YA+++ES+M ++Y +RDC L  IG LLDSKGYGI  P GSPLRD +++++L+LQ+   +  L +KWW +      C     G  P+E
Sbjct:  598 GSDVIPRAAATRLIAVVWWMFTQIIISSYTAQLAAFLTVERMSTPIESTQDLANQQKIRYGVLKSGSTMDFFRESKIPMYERMWSVMESSSPGVFVNSSREGIARVK--SGGYAYMMESSMLEYYLERDCELQSIGGLLDSKGYGIALPKGSPLRDILSRTVLQLQERTILEALKNKWWRDRREGPSC-----GPPPSE 789          

HSP 2 Score: 99.3673 bits (246), Expect = 1.120e-20
Identity = 48/121 (39.67%), Postives = 78/121 (64.46%), Query Frame = 3
Query: 1932 QAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAP--GLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNS-----ELDNQFNLLNSLWFTVGALVRQG 2273
            +A+L+++ +TI+Y R +VVDFT PFM LG+SIL  +  +E     L++FL P+S  VW+ ++ +Y  VS+ +  ++  +PYEW  +   D+          NQF +LNS WFT+G+L++QG
Sbjct:  478 EADLSLSAVTITYSRAEVVDFTLPFMHLGISILLARTSEETDKGSLWTFLEPLSLTVWISLLISYCIVSYSMHILAKFSPYEWYNLERIDERDFENIKNQKNQFTVLNSFWFTMGSLMQQG 598          

HSP 3 Score: 63.929 bits (154), Expect = 9.566e-10
Identity = 77/298 (25.84%), Postives = 117/298 (39.26%), Query Frame = 1
Query:  736 DFLIMASVHNMTDPQFSFIFTDWDASLVDI---AAFKINNKVNITTFSITDHSIM--ERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKR--------IEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGEL 1590
            + L  A   NM +P FS++FT+ D SL++      +  +   NIT   +  +  M   ++  T E V  +  SI  M                                R L   P     M C S+D W  G  +   +   +    +TG + F  N +R +     +    + F K G W++ R      R        I+P   ++  +   L+V   + +PFV  K GE            + GFCIDLL E+   L  N+TI  V+D  YG  +E     D S +WNG+IG L
Sbjct:  230 NLLQQARGLNMAEPPFSYVFTNTDLSLLEDFLNNMYGASFHCNITGLQLVKNDPMMKTQLALTSEAVYVVGMSIYRM--------------------------------RELGHAPRQSSIM-CDSHDIWSDGRIMNEGIRKLKLRNQLTGDVQFKSNGERDDIMYHGVGRINSQFVKLGNWSEKRGWNFDSRYANRWEFDIDPDSEDLEGL--HLRVVVYLEEPFVI-KTGE----------NQYEGFCIDLLNEMTQVLKFNYTIIEVQDGTYG--IE-----DESGRWNGIIGAL 474          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Celegans
Match: glr-4 (GLutamate Receptor family (AMPA) [Source:UniProtKB/TrEMBL;Acc:Q17697])

HSP 1 Score: 192.586 bits (488), Expect = 1.248e-49
Identity = 90/199 (45.23%), Postives = 137/199 (68.84%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNRE-VFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTE 4045
            G +  P+A++TR+++  WW FT IIIS+YTA LAAFLTVE++  PI+S +DLA Q  I+YG LK G++  FF ++ IP Y++M+  M+ +   VFV+S+ +G+ +V+  +G YA+++ES+M ++Y +RDC L  IG LLDSKGYGI  P GSPLRD +++++L+LQ+   +  L +KWW +      C     G  P+E
Sbjct:  598 GSDVIPRAAATRLIAVVWWMFTQIIISSYTAQLAAFLTVERMSTPIESTQDLANQQKIRYGVLKSGSTMDFFRESKIPMYERMWSVMESSSPGVFVNSSREGIARVK--SGGYAYMMESSMLEYYLERDCELQSIGGLLDSKGYGIALPKGSPLRDILSRTVLQLQERTILEALKNKWWRDRREGPSC-----GPPPSE 789          

HSP 2 Score: 98.5969 bits (244), Expect = 2.340e-20
Identity = 48/121 (39.67%), Postives = 78/121 (64.46%), Query Frame = 3
Query: 1932 QAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAP--GLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNS-----ELDNQFNLLNSLWFTVGALVRQG 2273
            +A+L+++ +TI+Y R +VVDFT PFM LG+SIL  +  +E     L++FL P+S  VW+ ++ +Y  VS+ +  ++  +PYEW  +   D+          NQF +LNS WFT+G+L++QG
Sbjct:  478 EADLSLSAVTITYSRAEVVDFTLPFMHLGISILLARTSEETDKGSLWTFLEPLSLTVWISLLISYCIVSYSMHILAKFSPYEWYNLERIDERDFENIKNQKNQFTVLNSFWFTMGSLMQQG 598          

HSP 3 Score: 63.5438 bits (153), Expect = 1.085e-9
Identity = 77/298 (25.84%), Postives = 117/298 (39.26%), Query Frame = 1
Query:  736 DFLIMASVHNMTDPQFSFIFTDWDASLVDI---AAFKINNKVNITTFSITDHSIM--ERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKR--------IEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGEL 1590
            + L  A   NM +P FS++FT+ D SL++      +  +   NIT   +  +  M   ++  T E V  +  SI  M                                R L   P     M C S+D W  G  +   +   +    +TG + F  N +R +     +    + F K G W++ R      R        I+P   ++  +   L+V   + +PFV  K GE            + GFCIDLL E+   L  N+TI  V+D  YG  +E     D S +WNG+IG L
Sbjct:  230 NLLQQARGLNMAEPPFSYVFTNTDLSLLEDFLNNMYGASFHCNITGLQLVKNDPMMKTQLALTSEAVYVVGMSIYRM--------------------------------RELGHAPRQSSIM-CDSHDIWSDGRIMNEGIRKLKLRNQLTGDVQFKSNGERDDIMYHGVGRINSQFVKLGNWSEKRGWNFDSRYANRWEFDIDPDSEDLEGL--HLRVVVYLEEPFVI-KTGE----------NQYEGFCIDLLNEMTQVLKFNYTIIEVQDGTYG--IE-----DESGRWNGIIGAL 474          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Celegans
Match: glr-1 (Glutamate receptor 1 [Source:UniProtKB/Swiss-Prot;Acc:P34299])

HSP 1 Score: 178.333 bits (451), Expect = 4.467e-45
Identity = 86/191 (45.03%), Postives = 131/191 (68.59%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKN-REVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRD-CSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDS 4018
            G +  P++ S R+ S AWWFFT+II+S+YTANLAAFLT+E++  PI+SVEDLA+Q+ IKYG   GG++ +FF+ + +  YQ+M+++M+     VFV S  +G+ +VR+  G+YAFLLE+  N++ + R  C  M++G  L+S GYGI  P GS  +DH+  +IL LQ+  ++  L +KWW +      CD+
Sbjct:  653 GTDILPRSISGRIASSAWWFFTMIIVSSYTANLAAFLTLEKMQAPIESVEDLAKQSKIKYGIQGGGSTASFFKYSSVQIYQRMWRYMESQVPPVFVASYAEGIERVRSHKGRYAFLLEATANEYENTRKPCDTMKVGANLNSIGYGIATPFGSDWKDHINLAILALQERGELKKLENKWWYDRG---QCDA 840          

HSP 2 Score: 114.39 bits (285), Expect = 3.238e-25
Identity = 52/117 (44.44%), Postives = 78/117 (66.67%), Query Frame = 3
Query: 1923 INTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTVGALVRQG 2273
            ++ +A   +A +TI+ ERE+VVDF+ PFM+ G+SI+  KP K+   +FSF+ P+S  +W+YII AYIGVS +IF +S  +PYEW           + N F++ N LWFT+ A ++QG
Sbjct:  537 LSGRAHAVVASLTINQERERVVDFSKPFMTTGISIMIKKPDKQEFSVFSFMQPLSTEIWMYIIFAYIGVSVVIFLVSRFSPYEWRVEETSRGGFTISNDFSVYNCLWFTLAAFMQQG 653          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Celegans
Match: glr-2 (Glutamate receptor 2 [Source:UniProtKB/Swiss-Prot;Acc:Q10914])

HSP 1 Score: 174.866 bits (442), Expect = 4.690e-44
Identity = 108/309 (34.95%), Postives = 163/309 (52.75%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-----------------KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYS-QRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKES-GSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHPDLTNSKSRERLNLLDHQSGNVNDVS 4321
            G +  P+A S R+ S  WWFFTLII+S+YTANLAAFLT+E++  PI+SVEDLA Q  I YG  +GG++  FFE +++P Y+KM+ FM                   +  +FV S   G+ KVR S GKYAFLLE   N++ S +R C  M++G  L++ GYGI    G+PLR  +  +IL L +  ++  L +KWW +      CD+  S G   + LN+ +       +    +   ++S+    V++  +K+           KE +   R    S +PL P +  S  R +   L ++     +VS
Sbjct:  611 GTDILPRAVSGRIASSCWWFFTLIIVSSYTANLAAFLTLERMTPPIESVEDLANQNKILYGVNEGGSTAAFFEDSIVPLYKKMWNFMVSTTQKQIELEKQSITNSTSNRIFVSSYADGIEKVRTSKGKYAFLLEETTNNYESGRRPCDTMKVGQNLNTLGYGIATKIGNPLRVSLNLAILYLSEKGELKKLENKWWYDRG---QCDTGTSDGGTSSSLNLSK----VAGIFYILLAGMVLSMCTALVEFLFRKN-----------KENREKERNRMRSSRPLKPGILASCERAKQKQLQNRRTKSEEVS 901          

HSP 2 Score: 110.923 bits (276), Expect = 3.923e-24
Identity = 51/118 (43.22%), Postives = 80/118 (67.80%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTVGALVRQG 2273
             +N  A++A+AP+TI+ ERE+ VDF+ PFM+ G+SI+  KP+K+   +FSF+ P+   +W++ +S+Y GVS  IF +S  +PYE  +I        + N+F L NSLWFT+ A ++QG
Sbjct:  495 LLNETADVAVAPLTITQERERAVDFSKPFMTTGISIMIKKPEKQEFNIFSFMEPLGMTIWIFTLSSYFGVSLTIFLVSWFSPYE-KRIEFKRGEFTVTNEFTLYNSLWFTLAAFMQQG 611          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Fly
Match: CG11155 (gene:FBgn0039927 transcript:FBtr0333701)

HSP 1 Score: 204.142 bits (518), Expect = 2.902e-53
Identity = 121/259 (46.72%), Postives = 164/259 (63.32%), Query Frame = 2
Query: 3467 PQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNR-EVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPC--DSKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKK 4234
            P+A+STR+V G W+FF LIIIS+YTANLAAFLTVE++I PI+S  DLA+QT I YGTL+GG++ TFF  + I  YQKM+++M+  +  VFV + E G+ +V    G YAFL+ES M D+  QRDC+L +IG LLDSKGYGI  P GSP RD ++ +IL+LQ+   I  L  KWW   N  + C  D K   S    L V  IGG+F++L+ G   A +V+I E   +     +         + +EL+FAM    S  +
Sbjct:  621 PKATSTRIVGGCWFFFCLIIISSYTANLAAFLTVERMISPIESASDLAEQTEISYGTLEGGSTMTFFRDSKIGIYQKMWRYMENRKTAVFVKTYEDGIKRVM--EGSYAFLMESTMLDYAVQRDCNLTQIGGLLDSKGYGIATPKGSPWRDKISLAILELQEKGIIQILYDKWWK--NTGDVCNRDDKSKESKANALGVENIGGVFVVLLCGLALAVVVAIFEFCWNSRKNLNTENQSLCSEMAEELRFAMHCHGSKSR 875          

HSP 2 Score: 141.739 bits (356), Expect = 1.406e-33
Identity = 136/521 (26.10%), Postives = 229/521 (43.95%), Query Frame = 1
Query:  145 LLNIGVIL--DASDDISRKVISLAVQNANKELARF-NYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGI--IRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVR-PLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR--FIVNQISIN*KRCSSASTLKHHDKIGI 1683
            ++ +G I   D  +         A+   NKE     N       E +  ++SF   +K C+ +  G+ A+  P   + + H+ S+C  + IPH+    R     +     I L P+      + Y+ I     W  + +I+  D  +    H   + ++   +RQ         +++LR + + + Y+  +VD     I  F        M D ++ ++FT +D    D+  F+ N+ VNIT F + D    +R  +    ++KL  + ++    S     E A M      FD+V     G+  + L   +   ++ + C S+ TW  G SL N + AA    G+TG + F E  +R  + L I++L +   QK G+W+ D  V +    +P+     NI    L V T   +P+V  KE     +N   N+  + GFCIDLL+ I   +G  + I LV DN YG  +  +TN      WNG++ EL+ +R    V  ++IN  R S     K    +GI
Sbjct:   42 VIRVGAIFTEDERESSIESAFKYAIYRINKEKTLLPNTQLVYDIEYVPRDDSFRTTKKVCSQLEAGVQAIFGPTDALLASHVQSICEAYDIPHI--EGRIDLEYNSKEFSINLYPS-HTLLTLAYRDIMVYLNWTKVAIIYEEDYGLFNLMHSSTETKAEMYIRQ---ASPDSYRQVLRAIRQKEIYK-IIVDTNPSHIKSFFRSILQLQMNDHRYHYMFTTFDLETYDLEDFRYNS-VNITAFRLVDVD-SKRYLEVINQMQKLQHNGLDTINGSPYIQTESALM------FDSVYAFANGLHFLNLDNHQNFYIKNLSCTSDQTWNDGISLYNQINAAITD-GLTGTVQFVE-GRRNIFKLDILKLKQEKIQKVGYWHPDDGVNIS---DPTAFYDSNIANITLVVMTREERPYVMVKED----KNLTGNLR-FEGFCIDLLKAIATQVGFQYKIELVPDNMYGVYIP-ETN-----SWNGIVQELMERRADLAVASMTINYARESVIDFTKPFMNLGI 531          

HSP 3 Score: 126.331 bits (316), Expect = 8.153e-29
Identity = 55/115 (47.83%), Postives = 83/115 (72.17%), Query Frame = 3
Query: 1932 QAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSEL-DNQFNLLNSLWFTVGALVRQG 2273
            +A+LA+A MTI+Y RE V+DFT PFM+LG+ ILF  P  +   LFSF++P++  +W+Y+++AYI VSF +F ++  +PYEW   HPC   +++ +NQF++ NS WF  G  +RQG
Sbjct:  502 RADLAVASMTINYARESVIDFTKPFMNLGIGILFKVPTSQPTRLFSFMNPLAIEIWLYVLAAYILVSFALFVMARFSPYEWKNPHPCYKETDIVENQFSISNSFWFITGTFLRQG 616          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Fly
Match: CG11155 (gene:FBgn0039927 transcript:FBtr0089195)

HSP 1 Score: 204.142 bits (518), Expect = 2.902e-53
Identity = 121/259 (46.72%), Postives = 164/259 (63.32%), Query Frame = 2
Query: 3467 PQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNR-EVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPC--DSKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKK 4234
            P+A+STR+V G W+FF LIIIS+YTANLAAFLTVE++I PI+S  DLA+QT I YGTL+GG++ TFF  + I  YQKM+++M+  +  VFV + E G+ +V    G YAFL+ES M D+  QRDC+L +IG LLDSKGYGI  P GSP RD ++ +IL+LQ+   I  L  KWW   N  + C  D K   S    L V  IGG+F++L+ G   A +V+I E   +     +         + +EL+FAM    S  +
Sbjct:  621 PKATSTRIVGGCWFFFCLIIISSYTANLAAFLTVERMISPIESASDLAEQTEISYGTLEGGSTMTFFRDSKIGIYQKMWRYMENRKTAVFVKTYEDGIKRVM--EGSYAFLMESTMLDYAVQRDCNLTQIGGLLDSKGYGIATPKGSPWRDKISLAILELQEKGIIQILYDKWWK--NTGDVCNRDDKSKESKANALGVENIGGVFVVLLCGLALAVVVAIFEFCWNSRKNLNTENQSLCSEMAEELRFAMHCHGSKSR 875          

HSP 2 Score: 141.739 bits (356), Expect = 1.406e-33
Identity = 136/521 (26.10%), Postives = 229/521 (43.95%), Query Frame = 1
Query:  145 LLNIGVIL--DASDDISRKVISLAVQNANKELARF-NYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGI--IRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVR-PLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR--FIVNQISIN*KRCSSASTLKHHDKIGI 1683
            ++ +G I   D  +         A+   NKE     N       E +  ++SF   +K C+ +  G+ A+  P   + + H+ S+C  + IPH+    R     +     I L P+      + Y+ I     W  + +I+  D  +    H   + ++   +RQ         +++LR + + + Y+  +VD     I  F        M D ++ ++FT +D    D+  F+ N+ VNIT F + D    +R  +    ++KL  + ++    S     E A M      FD+V     G+  + L   +   ++ + C S+ TW  G SL N + AA    G+TG + F E  +R  + L I++L +   QK G+W+ D  V +    +P+     NI    L V T   +P+V  KE     +N   N+  + GFCIDLL+ I   +G  + I LV DN YG  +  +TN      WNG++ EL+ +R    V  ++IN  R S     K    +GI
Sbjct:   42 VIRVGAIFTEDERESSIESAFKYAIYRINKEKTLLPNTQLVYDIEYVPRDDSFRTTKKVCSQLEAGVQAIFGPTDALLASHVQSICEAYDIPHI--EGRIDLEYNSKEFSINLYPS-HTLLTLAYRDIMVYLNWTKVAIIYEEDYGLFNLMHSSTETKAEMYIRQ---ASPDSYRQVLRAIRQKEIYK-IIVDTNPSHIKSFFRSILQLQMNDHRYHYMFTTFDLETYDLEDFRYNS-VNITAFRLVDVD-SKRYLEVINQMQKLQHNGLDTINGSPYIQTESALM------FDSVYAFANGLHFLNLDNHQNFYIKNLSCTSDQTWNDGISLYNQINAAITD-GLTGTVQFVE-GRRNIFKLDILKLKQEKIQKVGYWHPDDGVNIS---DPTAFYDSNIANITLVVMTREERPYVMVKED----KNLTGNLR-FEGFCIDLLKAIATQVGFQYKIELVPDNMYGVYIP-ETN-----SWNGIVQELMERRADLAVASMTINYARESVIDFTKPFMNLGI 531          

HSP 3 Score: 126.331 bits (316), Expect = 8.153e-29
Identity = 55/115 (47.83%), Postives = 83/115 (72.17%), Query Frame = 3
Query: 1932 QAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSEL-DNQFNLLNSLWFTVGALVRQG 2273
            +A+LA+A MTI+Y RE V+DFT PFM+LG+ ILF  P  +   LFSF++P++  +W+Y+++AYI VSF +F ++  +PYEW   HPC   +++ +NQF++ NS WF  G  +RQG
Sbjct:  502 RADLAVASMTINYARESVIDFTKPFMNLGIGILFKVPTSQPTRLFSFMNPLAIEIWLYVLAAYILVSFALFVMARFSPYEWKNPHPCYKETDIVENQFSISNSFWFITGTFLRQG 616          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Fly
Match: KaiR1D (gene:FBgn0038837 transcript:FBtr0083987)

HSP 1 Score: 202.601 bits (514), Expect = 4.828e-53
Identity = 109/229 (47.60%), Postives = 152/229 (66.38%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNR-EVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPC--DSKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIEL 4129
            G +F P+A STRMV+G WWFFTLI+IS+YTANLAAFLTVE++  PI+S EDLA+QT IKYG LKGG++  FF  + I TYQ+M+ FM+  R  VF  S  +GV +V    G YAFL+ES   ++ ++R+C L ++G +LD+K YGI  P  SP R  +   ILKLQ+  K+  L  KWW E      C  ++ +S S   EL +  +GG+F++L+GG   A ++++ E 
Sbjct:  606 GCDFLPKALSTRMVAGIWWFFTLIMISSYTANLAAFLTVERMDSPIESAEDLAKQTRIKYGALKGGSTAAFFRDSKISTYQRMWSFMESARPSVFTASNGEGVERVAKGKGSYAFLMESTSIEYVTERNCELTQVGGMLDTKSYGIATPPNSPYRTAINSVILKLQEEGKLHILKTKWWKEKRGGGKCRVETSKSSSAANELGLANVGGVFVVLMGGMGVACVIAVCEF 834          

HSP 2 Score: 152.14 bits (383), Expect = 5.764e-37
Identity = 132/498 (26.51%), Postives = 222/498 (44.58%), Query Frame = 1
Query:  145 LLNIGVILDASDDISRKVISLAVQNANKE---LARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRI--------EPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            ++ IG +   +DD        AV   N +   L R     Q+  E +   +SF+A ++ C L+  G+ A+  P +   + H+ S+C    IPHL    R+   L R    + L P+ +  +K  Y  I +   W+   +I+ +++ I++ + LL     + F +      D    + LL+++ K+    + ++D    +I + L  A    M     S++ T  D   V++  F+     NIT F + +  I+  + + +   EK L     +              S+ A+++D V+   K +  L  S+ + + P+ C    TW+ G SLIN M   E + G+T  I F+    R ++ L I+ELT  G +K G WN      LP  I        +   +E     + L VTTI+S P+   KE        D     + G+ +DL+ EI   LG N+ I LV D  YG ++   T      +WNGMI EL+ +R
Sbjct:   29 IIKIGGLFHPADDHQELAFRQAVDRINADRSILPRSKLVAQI--ERISPFDSFHAGKRVCGLLNIGVAAIFGPQSSHTASHVQSICDNMEIPHLEN--RWDYRLRRESCLVNLYPHPNTLSKA-YVDIVRHWGWKTFTIIYENNDGIVRLQELLKAHGMTPFPITVRQLSDSGDYRPLLKQI-KNSAEAHIVLDCSTERIHEVLKQAQQIGMMSDYHSYLVTSLDLHTVNLDEFRYGG-TNITGFRLINEKIVSDVVRQWSIDEKGLLRSANLTTVR----------SETALMYDAVHLFAKALHDLDTSQQIDIHPISCDGQSTWQHGFSLINYMKIVE-MKGLTNVIKFDHQGFRTDFMLDIVELTPAGIRKIGTWNS----TLPDGINFTRTFSQKQQEIEANLKNKTLVVTTILSNPYCMRKESAIPLSGNDQ----FEGYAVDLIHEISKSLGFNYKIQLVPDGSYG-SLNKLTG-----EWNGMIRELLEQR 494          

HSP 3 Score: 132.88 bits (333), Expect = 5.967e-31
Identity = 59/118 (50.00%), Postives = 90/118 (76.27%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTVGALVRQG 2273
             +  +A+LA+A +TI++ERE+ VDFTTPFM+LG+SIL+ KP K+ P LFSFLSP+S  VW+Y+ +AY+GVS ++F ++  TPYEW   +      ++++QF LLN +WF +G+L++QG
Sbjct:  490 LLEQRADLAIADLTITFEREQAVDFTTPFMNLGVSILYRKPIKQPPNLFSFLSPLSLDVWIYMATAYLGVSVLLFILAKFTPYEW-PAYTDAHGEKVESQFTLLNCMWFAIGSLMQQG 606          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Fly
Match: Grik (gene:FBgn0038840 transcript:FBtr0335015)

HSP 1 Score: 191.815 bits (486), Expect = 2.264e-49
Identity = 98/266 (36.84%), Postives = 165/266 (62.03%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQ-QTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHP 4246
            G E  P+A STR V+ +WWFFTLI++S+YTANLAAFLTVE L+ PI   +DL++ +  + YG   GG +F FF+++  PTYQ+M++FM+ N +   ++ ++GV++V NSN  YAFL+ES   ++ ++R C+L ++G LLD KGYGI      P RD +++++L++Q+   +  +  KWW E      C   +  S    L +  +GG+F+++  G  F   VS++E+ +    +  + ++ F   L  E++F ++   ++K   +P
Sbjct:  604 GSELAPKAYSTRAVAASWWFFTLILVSSYTANLAAFLTVESLVTPINDADDLSKNKGGVNYGAKIGGATFNFFKESNYPTYQRMYEFMRDNPQYMTNTNQEGVDRVENSN--YAFLMESTTIEYITERRCTLTQVGALLDEKGYGIAMRKNWPYRDTLSQAVLEMQEQGLLTKMKTKWWQEKRGGGACSDADEDSGAVALEISNLGGVFLVMGVGSFFGIFVSLLEMVLGVKERSDENQVSFKTELLDEIRFILQCSGNTKAVKYP 867          

HSP 2 Score: 132.494 bits (332), Expect = 1.094e-30
Identity = 117/494 (23.68%), Postives = 223/494 (45.14%), Query Frame = 1
Query:  151 NIGVILDASDDISRKVISLAVQNANKELARFNYAFQVK--TETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKN---KKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWN-DDRF----VVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            N+G++ + +D    K+  LA+  AN+E    N   Q+   + +++  NSF   +K C ++R  LVA+  P + + +RH  S+C    +P L T   F   L      I L P+       +   +  +  W    +I+ S   +     LL  + +         ++L  N   + +LR +  +  + + +V      + +F   A    +    + +I  + D   +D+  ++ +   NIT   +        ++   E V+++ +++ E E+  +  ++     +  A+++D V  L +   + +  RP+    + C  +  W  G +L+N M +   L G+TG I F+    R ++ L ++EL  +G QK G W+ +D F          +EP +  + N  +   V T IS+P+   KE     +  D     + GF I+L+ E+   LG ++T  L +DNKYG  ++ +T      +WNGM+ E+I  R
Sbjct:   35 NVGLVYENTDPDLEKIFHLAISKANEE----NEDLQLHGVSVSIEPGNSFETSKKLCKMLRQNLVAVFGPTSNLAARHAMSICDAKELPFLDTRWDFGAQLPT----INLHPHPATLGVALRDMVVALG-WESFTIIYESGEYLPTVRELLQMYGTAGPTVTVRRYELDLNGNYRNVLRRIRNADDFSFVVVG-SMATLPEFFKQAQQVGLVTSDYRYIIGNLDWHTMDLEPYQ-HAGTNITGLRL--------VSPDSEQVQEVAKALYESEEPFQ--NVSCPLTNSMALVYDGVQLLAE-TYKHVNFRPVA---LSCNDDSAWDKGYTLVNYMKSL-TLNGLTGPIRFDYEGLRTDFKLEVIELAVSGMQKIGQWSGEDGFQENRPAPAHSLEPDMRSLVN--KSFVVITAISEPYGMLKETSEKLEGNDQ----FEGFGIELIDELSKKLGFSYTWRLQEDNKYG-GIDPKTG-----EWNGMLREIIDSR 490          

HSP 3 Score: 127.487 bits (319), Expect = 3.132e-29
Identity = 58/118 (49.15%), Postives = 87/118 (73.73%), Query Frame = 3
Query: 1923 INTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC-DDNSELDNQFNLLNSLWFTVGALVRQG 2273
            I+++A++ +  +T++ ERE  VDFT PFMSLG+ ILF KP KE P LFSF+SP S  VW+++  AY+GVS  +F +  ++P EW   +PC ++ +EL+NQF+  N LWF++GAL++QG
Sbjct:  487 IDSRADMGITDLTMTSERESGVDFTIPFMSLGIGILFRKPMKEPPKLFSFMSPFSGEVWLWLGLAYMGVSISMFVLGRLSPAEWDNPYPCIEEPTELENQFSFANCLWFSIGALLQQG 604          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Fly
Match: Ekar (gene:FBgn0039916 transcript:FBtr0110906)

HSP 1 Score: 187.578 bits (475), Expect = 4.696e-48
Identity = 94/188 (50.00%), Postives = 128/188 (68.09%), Query Frame = 2
Query: 3467 PQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKES 4027
            P+A STR++S  W FF+LII+++YTANLAAFLT E++I PI++ EDLA QT I YGTL  G++ TFF  ++I TY+K+++ M  K    F  + E G+ +V  + G YAFL+ES M D+  QRDC+L +IG LLD+KGYGI  P GSP RD ++ +IL+LQ+   I  L  KWW   N  E C  K +
Sbjct:  599 PRAMSTRIISSTWGFFSLIIVASYTANLAAFLTTERMINPIENAEDLASQTEISYGTLDSGSTMTFFRDSVIETYKKIWRSMDNKKPSAFTTTYEDGIKRV--NQGNYAFLMESTMLDYIVQRDCNLTQIGGLLDTKGYGIATPKGSPWRDKISLAILELQERGDIQMLYDKWWK--NTDETCTRKNT 782          

HSP 2 Score: 149.058 bits (375), Expect = 6.149e-36
Identity = 135/482 (28.01%), Postives = 226/482 (46.89%), Query Frame = 1
Query:  268 ETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVE-----MEKTSKKCDIEKAFM--SKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSV-VEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR--FIVNQISIN*KRCSSASTLKHHDKIGI 1683
            E ++  +SF  ++K C LIR G+ A+ SP   V + HI+S+C    IP++  S     S++ YP          Q     +  + Q   W    ++H  +N I+    L   F     MRQ V  D       L E  K K+    ++D     I+  L       M + ++ ++FT +D    D+  FK  N VNIT+F + D + +         V+++L+ I        +K      I+K+ +  S+PA++FD+V     G+  L +S  L +  + C+  ++W  G SLIN + A E   G+TG I F ++ QR  + L +++L ++   K G W     + +    EPS+  + G++   L V TI+  P+V    G    +N+  N E + GFC+D+L  I  ++G ++ + LV D KYG       +P+  E WNGM+ +L+  +    V  ++I   R S     K    +GI
Sbjct:   66 EYVNRFDSFETVQKVCKLIRVGVQAVFSPTDSVLATHINSICDALDIPNIGRSAH-DFSINVYP--------SKQLVNYAFNDVIQYLNWTRFGILHEKENGIINLHQLSRSFHGEVHMRQ-VSRD--SYVSALNEF-KGKEIHNIIIDTNSNGISILLKNILQQQMNEYKYHYLFTSFDLETYDLEDFKY-NFVNITSFRLVDTADV--------GVKQILKDIGLYSHHIFKKPYLNLHIKKSTILESEPALMFDSVYVFAIGLQTLEQSHSLTLLNISCEEENSWDGGLSLINYLNAVE-WKGLTGPIQF-KDGQRVQFKLDLIKLKQHSIVKVGEWTPHGHLNI---TEPSMFFDAGSMNVTLVVITILETPYVMMHYG----KNFTGN-ERFYGFCVDILETISREVGFDYILDLVPDRKYGAK-----DPETGE-WNGMVAQLMKYKADLAVGSMTITYARESVIDFTKPFMNLGI 509          

HSP 3 Score: 118.242 bits (295), Expect = 2.588e-26
Identity = 53/114 (46.49%), Postives = 80/114 (70.18%), Query Frame = 3
Query: 1932 QAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCD-DNSELDNQFNLLNSLWFTVGALVRQ 2270
            +A+LA+  MTI+Y RE V+DFT PFM+LG+SILF  P  E   LFSF++P++  +W+Y++ AY  VS  I+ +  ++P EW  I+ CD +N  + NQF+L +S WFT+G  ++Q
Sbjct:  480 KADLAVGSMTITYARESVIDFTKPFMNLGISILFKVPTSEPTRLFSFMNPLAIEIWIYVLIAYFLVSLCIYIVGKLSPIEWKCINACDLENISIGNQFSLTDSFWFTIGTFMQQ 593          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Zebrafish
Match: si:ch211-264f5.8 (si:ch211-264f5.8 [Source:ZFIN;Acc:ZDB-GENE-081104-209])

HSP 1 Score: 212.231 bits (539), Expect = 1.560e-60
Identity = 104/194 (53.61%), Postives = 140/194 (72.16%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+S EDLA QTNI+YGT++GG++ TFF  +   TYQ+M+ +M  K   VFV STE+G+ +V NS  KYAFLLES MN+++ + +C+L +IG LLD+KGYGIG P GSP RD +T  IL+LQ++ ++  L  +WW      +  D +  G
Sbjct:   58 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESAEDLADQTNIQYGTIQGGSTMTFFMNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNS--KYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLGILQLQENNRLEILKRRWWEGGKCLKEEDHRAKG 249          

HSP 2 Score: 53.9138 bits (128), Expect = 7.572e-7
Identity = 23/55 (41.82%), Postives = 35/55 (63.64%), Query Frame = 3
Query: 2115 AYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
            AY+ VS ++F  + ++PYEW    PC  +    L+NQ+ L NSLWF +G  ++QG
Sbjct:    4 AYLAVSCVLFLAARLSPYEWYNPFPCWRERRDLLENQYTLGNSLWFPIGGFMQQG 58          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Zebrafish
Match: grik5 (glutamate receptor, ionotropic, kainate 5 [Source:ZFIN;Acc:ZDB-GENE-070821-6])

HSP 1 Score: 207.608 bits (527), Expect = 1.907e-55
Identity = 101/194 (52.06%), Postives = 139/194 (71.65%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+S +DLA QTNI+YGT+ GG++ TFF  +   TYQ+M+ +M  K   VFV STE+G+ +V NS  KYAFLLES MN+++   +C+L +IG LLD+KGYGIG P GSP RD ++ ++L+LQ++ ++  L  +WW      +  D +  G
Sbjct:  339 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESPDDLADQTNIQYGTIHGGSTMTFFMNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVVNS--KYAFLLESTMNEYHRSLNCNLTQIGGLLDTKGYGIGMPLGSPFRDELSLAVLQLQENNRLEILKRRWWEGGKCPKEEDHRAKG 530          

HSP 2 Score: 123.25 bits (308), Expect = 4.447e-28
Identity = 58/120 (48.33%), Postives = 82/120 (68.33%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC  +    L+NQ+ L NSLWF VG  ++QG
Sbjct:  220 LINRKADLAVAAFTITSEREKVIDFSKPFMTLGISILYRVHIGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLSARLSPYEWYNPHPCLRERRDLLENQYTLGNSLWFPVGGFMQQG 339          

HSP 3 Score: 112.464 bits (280), Expect = 1.375e-24
Identity = 69/202 (34.16%), Postives = 114/202 (56.44%), Query Frame = 1
Query: 1003 AILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFW-NDDRFVVLPKRIEPSVVE-IGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            A+LFD V+ +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E   G+TG++ FN   QR NY L I+E    G ++ G W +++  ++    ++ +V E + N  + L +TTI+  P+V  K    + Q ++ N E + GFC+D+LRE+ + L   + I LV D  YG        P+GS  W GM+GELI ++
Sbjct:   37 ALLFDAVHVVVSAVQELNRSQEIGVKPLSCTSPQIWQHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLHILEKHRGGHKEIGIWYSNNTLLMNSTSLDINVSETLAN--KSLIITTILENPYVMRKI---NYQEFEGN-EQYEGFCVDMLRELSDILKFTYRIKLVDDGLYGAP-----EPNGS--WTGMVGELINRK 224          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Zebrafish
Match: grik4 (glutamate receptor, ionotropic, kainate 4 [Source:ZFIN;Acc:ZDB-GENE-070821-5])

HSP 1 Score: 211.46 bits (537), Expect = 2.215e-55
Identity = 104/194 (53.61%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG++ TFF+ +   TYQ+M+ FM  K   VFV STE+G+ +V NSN  YA+LLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ+  ++  L  KWW+     +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESVDDLADQTAIEYGTMHGGSTMTFFQNSRYQTYQRMWNFMYSKQPSVFVKSTEEGIARVLNSN--YAYLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPLGSVYRDEFDLAILRLQEENRLEILKRKWWDGGKCPKEEDHRAKG 799          

HSP 2 Score: 130.183 bits (326), Expect = 7.927e-30
Identity = 127/501 (25.35%), Postives = 227/501 (45.31%), Query Frame = 1
Query:  154 IGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHI-SSLCSKFHIPHL------VTSCRFPE--SLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKP---AILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQ-PFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            I  ILD   + SR     I+LA  + N+   R      +V    L  ++ +      C ++  G+VA++ P A   S  I S++C +  +P++      +   +FP   +LD  P +  ++      A ++  + +         LI +  +C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    +VD         L  AS   M    +++I T  + SL+ +     + +VNI  FS+          +T+   +  + S+       + CD    F   P   A+LFD V+ +   +  L +S+ +    + CKS+  W  G+SL+N +   E L G+TG I FN   QR NY L IM+ + +G ++ G W+ ++ + + +++ PS+     +       T I + P+V  +    + +  D     + GFC+D+L+E+ + L   + I LV D  YG        P  +  W GM+GELI+++
Sbjct:   27 IAAILDDPMECSRGERLAITLAKDSINRSSNRSTTGKLEVDIFELLRDSEYETGETMCQIMSKGVVAVLGPSASPASNSIISNICGEKEVPYVKVAPEDILKVQFPRFTTLDLRPTNTDISM---AVAGLLTFFNSSTSC-----LICAQADCLLNLEVLLRQFLISKETLSVRMLDDSQDPTPLLKEI-RDDKTATIIVDANATMSHVILERASELGMLSVYYTYILTSLEFSLLRLDDLA-DQRVNIVGFSV--------FNRTHPFFQDFVLSL--NRSWQENCD-HAPFAGTPLSSALLFDAVHTVVAAVQELNRSQNVGATQLSCKSSKIWEHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALRIMQNSRDGLRQIGQWHSEQGLSMERKL-PSLNVTDTLFNTTLTITTILENPYVMLRANHQELEGNDR----YEGFCVDMLKELADILKFKYRIRLVGDGVYGV-------PGANGTWTGMVGELISRK 493          

HSP 3 Score: 120.939 bits (302), Expect = 5.151e-27
Identity = 57/120 (47.50%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             I+ +A+LA+A +TI+ EREKV+DF+ PFM+LG+SI++       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     S L NQ++L NS WF VG  ++QG
Sbjct:  489 LISRKADLAVAALTITAEREKVIDFSKPFMTLGISIMYRVHIGRRPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYNPHPCLKGRCSLLINQYSLGNSFWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Zebrafish
Match: grik4 (glutamate receptor, ionotropic, kainate 4 [Source:ZFIN;Acc:ZDB-GENE-070821-5])

HSP 1 Score: 211.46 bits (537), Expect = 2.215e-55
Identity = 104/194 (53.61%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG++ TFF+ +   TYQ+M+ FM  K   VFV STE+G+ +V NSN  YA+LLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ+  ++  L  KWW+     +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESVDDLADQTAIEYGTMHGGSTMTFFQNSRYQTYQRMWNFMYSKQPSVFVKSTEEGIARVLNSN--YAYLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPLGSVYRDEFDLAILRLQEENRLEILKRKWWDGGKCPKEEDHRAKG 799          

HSP 2 Score: 130.183 bits (326), Expect = 7.927e-30
Identity = 127/501 (25.35%), Postives = 227/501 (45.31%), Query Frame = 1
Query:  154 IGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHI-SSLCSKFHIPHL------VTSCRFPE--SLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKP---AILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQ-PFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            I  ILD   + SR     I+LA  + N+   R      +V    L  ++ +      C ++  G+VA++ P A   S  I S++C +  +P++      +   +FP   +LD  P +  ++      A ++  + +         LI +  +C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    +VD         L  AS   M    +++I T  + SL+ +     + +VNI  FS+          +T+   +  + S+       + CD    F   P   A+LFD V+ +   +  L +S+ +    + CKS+  W  G+SL+N +   E L G+TG I FN   QR NY L IM+ + +G ++ G W+ ++ + + +++ PS+     +       T I + P+V  +    + +  D     + GFC+D+L+E+ + L   + I LV D  YG        P  +  W GM+GELI+++
Sbjct:   27 IAAILDDPMECSRGERLAITLAKDSINRSSNRSTTGKLEVDIFELLRDSEYETGETMCQIMSKGVVAVLGPSASPASNSIISNICGEKEVPYVKVAPEDILKVQFPRFTTLDLRPTNTDISM---AVAGLLTFFNSSTSC-----LICAQADCLLNLEVLLRQFLISKETLSVRMLDDSQDPTPLLKEI-RDDKTATIIVDANATMSHVILERASELGMLSVYYTYILTSLEFSLLRLDDLA-DQRVNIVGFSV--------FNRTHPFFQDFVLSL--NRSWQENCD-HAPFAGTPLSSALLFDAVHTVVAAVQELNRSQNVGATQLSCKSSKIWEHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALRIMQNSRDGLRQIGQWHSEQGLSMERKL-PSLNVTDTLFNTTLTITTILENPYVMLRANHQELEGNDR----YEGFCVDMLKELADILKFKYRIRLVGDGVYGV-------PGANGTWTGMVGELISRK 493          

HSP 3 Score: 120.939 bits (302), Expect = 5.151e-27
Identity = 57/120 (47.50%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             I+ +A+LA+A +TI+ EREKV+DF+ PFM+LG+SI++       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     S L NQ++L NS WF VG  ++QG
Sbjct:  489 LISRKADLAVAALTITAEREKVIDFSKPFMTLGISIMYRVHIGRRPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYNPHPCLKGRCSLLINQYSLGNSFWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Zebrafish
Match: grik5 (glutamate receptor, ionotropic, kainate 5 [Source:ZFIN;Acc:ZDB-GENE-070821-6])

HSP 1 Score: 208.764 bits (530), Expect = 3.403e-54
Identity = 101/194 (52.06%), Postives = 139/194 (71.65%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+S +DLA QTNI+YGT+ GG++ TFF  +   TYQ+M+ +M  K   VFV STE+G+ +V NS  KYAFLLES MN+++   +C+L +IG LLD+KGYGIG P GSP RD ++ ++L+LQ++ ++  L  +WW      +  D +  G
Sbjct:  620 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESPDDLADQTNIQYGTIHGGSTMTFFMNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVVNS--KYAFLLESTMNEYHRSLNCNLTQIGGLLDTKGYGIGMPLGSPFRDELSLAVLQLQENNRLEILKRRWWEGGKCPKEEDHRAKG 811          

HSP 2 Score: 140.584 bits (353), Expect = 5.756e-33
Identity = 143/531 (26.93%), Postives = 244/531 (45.95%), Query Frame = 1
Query:   82 FGFVIFFLSICPISTKELSKKL--LNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHI-SSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIM--ASVHNMTDPQFSFIFTDWDASLV---DIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKK-CDIEK--AFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFW-NDDRFVVLPKRIEPSVVE-IGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            F F++  +   P S+   +  L  + +  ILD S    R     ++LA +N N  +     A  +V    L  ++ +      C ++  G+V++I P +   S  I S +C +  IPH+      PE   R P      + L P+ +  +  I   +     +    L+ +   C+L+ E L+ +F  SR  +   +  D      LL+E+ +  K    ++D        +LI+  AS   MT   + +I T  D  L+   DI    ++ + NI  FS+  T H                LE I  +  + ++ CDI          A+LFD V+ +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E   G+TG++ FN   QR NY L I+E    G ++ G W +++  ++    ++ +V E + N  + L +TTI+  P+V  K    + Q ++ N E + GFC+D+LRE+ + L   + I LV D  YG        P+GS  W GM+GELI ++
Sbjct:   13 FRFLLVTVVTMPPSSLSQTPVLSSVRMAAILDDSSVCGRGERLALALARENINSVMENPARARVEVDIFELQKDSQYETTDTMCQILPKGVVSVIGPASSPASGSIVSHICGEKEIPHVKIG---PEETPRLPYLRFASVTLYPSNEDLSLAIGSILRSFS-YPSASLVCAKAECLLRLEELIRRFLISRETLSIRMLDDNLDPTPLLKEI-RDDKIATIIIDAN--ASISYLILKKASELGMTSAFYKYILTTMDFPLLWLDDI----VDEQSNIVGFSMFNTTHPFY-------------LEFIRSLNLSWREGCDINPYPGPALSSALLFDAVHVVVSAVQELNRSQEIGVKPLSCTSPQIWQHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLHILEKHRGGHKEIGIWYSNNTLLMNSTSLDINVSETLAN--KSLIITTILENPYVMRKI---NYQEFEGN-EQYEGFCVDMLRELSDILKFTYRIKLVDDGLYGAP-----EPNGS--WTGMVGELINRK 505          

HSP 3 Score: 123.25 bits (308), Expect = 1.194e-27
Identity = 58/120 (48.33%), Postives = 82/120 (68.33%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC  +    L+NQ+ L NSLWF VG  ++QG
Sbjct:  501 LINRKADLAVAAFTITSEREKVIDFSKPFMTLGISILYRVHIGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLSARLSPYEWYNPHPCLRERRDLLENQYTLGNSLWFPVGGFMQQG 620          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Xenopus
Match: GRIK4 (glutamate receptor, ionotropic, kainate 4 [Source:NCBI gene;Acc:100490768])

HSP 1 Score: 211.846 bits (538), Expect = 2.213e-57
Identity = 104/194 (53.61%), Postives = 139/194 (71.65%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMK-KNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L ++G LLD+KGYGIG P GS  RD    +IL+LQ++  + +L  KWW+     +  D +  G
Sbjct:  316 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMDVPIESVDDLADQTTIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMHSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQVGGLLDTKGYGIGMPVGSAFRDEFDLAILQLQENNCLEDLKRKWWDGGKCPKEEDHRAKG 507          

HSP 2 Score: 123.635 bits (309), Expect = 3.095e-28
Identity = 58/120 (48.33%), Postives = 84/120 (70.00%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I+ +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  197 LISRKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 316          

HSP 3 Score: 120.939 bits (302), Expect = 2.221e-27
Identity = 67/202 (33.17%), Postives = 109/202 (53.96%), Query Frame = 1
Query:  997 KPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
              A+LFD V  +   +  L +S+ + V+P+ C S+  W+ G+SL+N +   E L G+TG + FN   QR NY++ IM+ T+ GFQ+ G W+    + + KR          + + L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI+++
Sbjct:   12 SAALLFDAVYGIVSAVQELNRSQEIGVKPLSCGSSQIWQHGTSLMNYLRMVE-LEGLTGHVEFNSKGQRSNYDMKIMQYTKTGFQEIGQWHVTNGLNMDKRYFSKNASDTLVNKTLIVTTILENPYLMLKANHQELEGNDR----YEGFCVDMLKELAAILRFNYKIRLVADGVYGV-------PELNGTWTGMVGELISRK 201          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Xenopus
Match: ogfr (opioid growth factor receptor [Source:Xenbase;Acc:XB-GENE-997410])

HSP 1 Score: 212.616 bits (540), Expect = 6.736e-57
Identity = 103/194 (53.09%), Postives = 141/194 (72.68%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMK-KNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+S +DLA QTNI+YGT+ GG++ TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NS  +YAFLLES MN+++ + +C+L +IG LLD+KGYGIG P GSP RD +T +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  322 GSEVMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESADDLADQTNIEYGTIHGGSTMTFFQNSRYQTYQRMWNYMNSKQPSVFVKSTEEGIARVLNS--RYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPYRDEITLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 513          

HSP 2 Score: 125.561 bits (314), Expect = 1.635e-28
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC  + +  L+NQ+ L NSLWF VG  ++QG
Sbjct:  203 LINRKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVQMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRERHHILENQYTLGNSLWFPVGGFMQQG 322          

HSP 3 Score: 103.219 bits (256), Expect = 1.423e-21
Identity = 65/204 (31.86%), Postives = 110/204 (53.92%), Query Frame = 1
Query: 1003 AILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVL-PKRIEPSVVE-IGNIVRPLKVTTIISQPFVFYKEGEPDRQNYD--TNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            A++FD V+ +   +  L +S+ + V+ + C ++  W+ G+SL+N +   E   G+TG++ FN   QR NY L I+E   +G ++ G W  +R + +    ++ S  E + N  + L VTTI+  P++  K       NY   +  + + GFC ++L E+   L  N+ I LV+D  YG        P+GS  W GM+GELI ++
Sbjct:   20 ALMFDAVHVVVSAVRELNRSQEIGVKQLSCSTSQIWQHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLRILEKAGDGHREIGVWYSNRTLAMNATSLDLSTSETLEN--KTLVVTTILENPYMMRK------SNYRSLSGNDQYEGFCAEMLMELAALLRFNYKIKLVEDGLYGAP-----EPNGS--WTGMVGELINRK 207          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Xenopus
Match: ogfr (opioid growth factor receptor [Source:Xenbase;Acc:XB-GENE-997410])

HSP 1 Score: 213.772 bits (543), Expect = 4.912e-56
Identity = 103/194 (53.09%), Postives = 141/194 (72.68%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMK-KNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+S +DLA QTNI+YGT+ GG++ TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NS  +YAFLLES MN+++ + +C+L +IG LLD+KGYGIG P GSP RD +T +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  557 GSEVMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESADDLADQTNIEYGTIHGGSTMTFFQNSRYQTYQRMWNYMNSKQPSVFVKSTEEGIARVLNS--RYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPYRDEITLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 748          

HSP 2 Score: 125.561 bits (314), Expect = 3.058e-28
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC  + +  L+NQ+ L NSLWF VG  ++QG
Sbjct:  438 LINRKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVQMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRERHHILENQYTLGNSLWFPVGGFMQQG 557          

HSP 3 Score: 106.301 bits (264), Expect = 2.256e-22
Identity = 70/229 (30.57%), Postives = 118/229 (51.53%), Query Frame = 1
Query:  928 LLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVL-PKRIEPSVVE-IGNIVRPLKVTTIISQPFVFYKEGEPDRQNYD--TNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            +L+   E+  TS         M   A++FD V+ +   +  L +S+ + V+ + C ++  W+ G+SL+N +   E   G+TG++ FN   QR NY L I+E   +G ++ G W  +R + +    ++ S  E + N  + L VTTI+  P++  K       NY   +  + + GFC ++L E+   L  N+ I LV+D  YG        P+GS  W GM+GELI ++
Sbjct:  230 ILKKASELGMTSAHYKYILTTMLSAALMFDAVHVVVSAVRELNRSQEIGVKQLSCSTSQIWQHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLRILEKAGDGHREIGVWYSNRTLAMNATSLDLSTSETLEN--KTLVVTTILENPYMMRK------SNYRSLSGNDQYEGFCAEMLMELAALLRFNYKIKLVEDGLYGAP-----EPNGS--WTGMVGELINRK 442          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Xenopus
Match: GRIK4 (glutamate receptor, ionotropic, kainate 4 [Source:NCBI gene;Acc:100490768])

HSP 1 Score: 212.616 bits (540), Expect = 5.888e-56
Identity = 104/194 (53.61%), Postives = 139/194 (71.65%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMK-KNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L ++G LLD+KGYGIG P GS  RD    +IL+LQ++  + +L  KWW+     +  D +  G
Sbjct:  589 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMDVPIESVDDLADQTTIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMHSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQVGGLLDTKGYGIGMPVGSAFRDEFDLAILQLQENNCLEDLKRKWWDGGKCPKEEDHRAKG 780          

HSP 2 Score: 133.65 bits (335), Expect = 6.808e-31
Identity = 133/519 (25.63%), Postives = 225/519 (43.35%), Query Frame = 1
Query:  103 LSICPISTKELSKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSC----RFP----ESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMS---KPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            L I  IS   LS   L +  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G++ ++ P     S  I  ++C +  +PH+  S     RF      +L+ +P +  ++       +                LI +   C+L  E LL QF  S+  +   +  D      LL+E+ +  K    +V          L+ A+   M    +++IFT+            ++++VNI  FS+    HS  +    T +            +   + CD    F       A+LFD V  +   +  L +S+ + V+P+ C S+  W+ G+SL+N +   E L G+TG + FN   QR NY++ IM+ T+ GFQ+ G W+    + + KR          + + L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI+++
Sbjct:    2 LCITIISPSCLS---LTVSAILDDPMECSRGERLSITLAKNRINRAPERQGKARLEVDIFELLKDSEYETAETMCQILPKGVLGVLGPSYSPSSSSIISNICGEKEVPHIKVSTEEFLRFQYQRFTTLNLHPSNTDISAAVATLLRFFNS--------TTACLICAKAECLLNLEKLLRQFLISKDCLSVRMLDDTWDPTPLLKEI-RDDKTSTIIVHANASMSHTILMKAAELGMVTAYYTYIFTNLS---------PLDDRVNILGFSMFNQSHSYFKEFAHTLQ------------QSWEENCD-HSPFTGPALSAALLFDAVYGIVSAVQELNRSQEIGVKPLSCGSSQIWQHGTSLMNYLRMVE-LEGLTGHVEFNSKGQRSNYDMKIMQYTKTGFQEIGQWHVTNGLNMDKRYFSKNASDTLVNKTLIVTTILENPYLMLKANHQELEGNDR----YEGFCVDMLKELAAILRFNYKIRLVADGVYGV-------PELNGTWTGMVGELISRK 474          

HSP 3 Score: 123.635 bits (309), Expect = 9.305e-28
Identity = 58/120 (48.33%), Postives = 84/120 (70.00%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I+ +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  470 LISRKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 589          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Xenopus
Match: GRIK4 (glutamate receptor, ionotropic, kainate 4 [Source:NCBI gene;Acc:100490768])

HSP 1 Score: 212.616 bits (540), Expect = 6.406e-56
Identity = 104/194 (53.61%), Postives = 139/194 (71.65%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMK-KNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L ++G LLD+KGYGIG P GS  RD    +IL+LQ++  + +L  KWW+     +  D +  G
Sbjct:  589 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMDVPIESVDDLADQTTIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMHSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQVGGLLDTKGYGIGMPVGSAFRDEFDLAILQLQENNCLEDLKRKWWDGGKCPKEEDHRAKG 780          

HSP 2 Score: 134.035 bits (336), Expect = 5.074e-31
Identity = 133/519 (25.63%), Postives = 225/519 (43.35%), Query Frame = 1
Query:  103 LSICPISTKELSKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSC----RFP----ESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMS---KPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            L I  IS   LS   L +  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G++ ++ P     S  I  ++C +  +PH+  S     RF      +L+ +P +  ++       +                LI +   C+L  E LL QF  S+  +   +  D      LL+E+ +  K    +V          L+ A+   M    +++IFT+            ++++VNI  FS+    HS  +    T +            +   + CD    F       A+LFD V  +   +  L +S+ + V+P+ C S+  W+ G+SL+N +   E L G+TG + FN   QR NY++ IM+ T+ GFQ+ G W+    + + KR          + + L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI+++
Sbjct:    2 LCITIISPSCLS---LTVSAILDDPMECSRGERLSITLAKNRINRAPERQGKARLEVDIFELLKDSEYETAETMCQILPKGVLGVLGPSYSPSSSSIISNICGEKEVPHIKVSTEEFLRFQYQRFTTLNLHPSNTDISAAVATLLRFFNSTTA--------CLICAKAECLLNLEKLLRQFLISKDCLSVRMLDDTWDPTPLLKEI-RDDKTSTIIVHANASMSHTILMKAAELGMVTAYYTYIFTNLS---------PLDDRVNILGFSMFNQSHSYFKEFAHTLQ------------QSWEENCD-HSPFTGPALSAALLFDAVYGIVSAVQELNRSQEIGVKPLSCGSSQIWQHGTSLMNYLRMVE-LEGLTGHVEFNSKGQRSNYDMKIMQYTKTGFQEIGQWHVTNGLNMDKRYFSKNASDTLVNKTLIVTTILENPYLMLKANHQELEGNDR----YEGFCVDMLKELAAILRFNYKIRLVADGVYGV-------PELNGTWTGMVGELISRK 474          

HSP 3 Score: 123.635 bits (309), Expect = 9.121e-28
Identity = 58/120 (48.33%), Postives = 84/120 (70.00%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I+ +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  470 LISRKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 589          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Mouse
Match: Grik2 (glutamate receptor, ionotropic, kainate 2 (beta 2) [Source:MGI Symbol;Acc:MGI:95815])

HSP 1 Score: 202.216 bits (513), Expect = 2.483e-57
Identity = 102/199 (51.26%), Postives = 140/199 (70.35%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNRE-VFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTE 4045
            G E  P+A STR+V G WWFFTLIIIS+YTANLAAFLTVE++  PI S +DLA+QT I+YG ++ G + TFF+K+ I TY KM+ FM   R+ V V S E+G+ +V  S+  YAFL+ES   +F +QR+C+L +IG L+DSKGYG+G P GSP RD +T +IL+LQ+  K+  +  KWW      E  +SKE+ ++  +
Sbjct:   63 GSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVEDGATMTFFKKSKISTYDKMWAFMSSRRQSVLVKSNEEGIQRVLTSD--YAFLMESTTIEFVTQRNCNLTQIGGLIDSKGYGVGTPMGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEE-ESKEASALGVQ 258          

HSP 2 Score: 76.2554 bits (186), Expect = 4.378e-14
Identity = 32/61 (52.46%), Postives = 47/61 (77.05%), Query Frame = 3
Query: 2094 VWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSE-LDNQFNLLNSLWFTVGALVRQG 2273
            +W+YI+ AY+GVS ++F I+  +PYEW   HPC+ +S+ ++N F LLNS WF VGAL++QG
Sbjct:    3 IWMYILLAYLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQG 63          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Mouse
Match: Grik4 (glutamate receptor, ionotropic, kainate 4 [Source:MGI Symbol;Acc:MGI:95817])

HSP 1 Score: 212.231 bits (539), Expect = 1.254e-55
Identity = 105/194 (54.12%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 799          

HSP 2 Score: 151.369 bits (381), Expect = 2.443e-36
Identity = 134/505 (26.53%), Postives = 227/505 (44.95%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSCRFPESLDRYPMD----IQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKKCD----IEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            S   L I  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G+VA++ P +   S  I  ++C +  +PH   +   PE   R+ +     + L P+    +  +   +          LI +   C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    ++          L+ A+   M    +++IFT+ + SL  + +  ++++VNI  FSI    H+  +  +++              +   + CD       A  S  A+LFD V  +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E L G+TG I FN   QR NY L I++ T NGFQ+ G W+    + +  R+  S +        L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI ++
Sbjct:   21 SPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVA---PEEFVRFQLQRFTTLNLHPSNTDISVAVAGILNFFNC-TTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEI-RDDKTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSL-VDDRVNILGFSIFNQSHAFFQEFSQSLN------------QSWQENCDHVPFTGPALSS--ALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALKILQFTRNGFQQIGQWHVAEGLSMDSRLYASNISDSLFNTTLVVTTILENPYLMLKGNHQEMEGNDR----YEGFCVDMLKELAEILRFNYKIRLVGDGVYGV-------PEANGTWTGMVGELIARK 493          

HSP 3 Score: 122.479 bits (306), Expect = 1.749e-27
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I  +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  489 LIARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRRPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Mouse
Match: Grik4 (glutamate receptor, ionotropic, kainate 4 [Source:MGI Symbol;Acc:MGI:95817])

HSP 1 Score: 212.231 bits (539), Expect = 1.254e-55
Identity = 105/194 (54.12%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 799          

HSP 2 Score: 151.369 bits (381), Expect = 2.443e-36
Identity = 134/505 (26.53%), Postives = 227/505 (44.95%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSCRFPESLDRYPMD----IQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKKCD----IEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            S   L I  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G+VA++ P +   S  I  ++C +  +PH   +   PE   R+ +     + L P+    +  +   +          LI +   C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    ++          L+ A+   M    +++IFT+ + SL  + +  ++++VNI  FSI    H+  +  +++              +   + CD       A  S  A+LFD V  +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E L G+TG I FN   QR NY L I++ T NGFQ+ G W+    + +  R+  S +        L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI ++
Sbjct:   21 SPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVA---PEEFVRFQLQRFTTLNLHPSNTDISVAVAGILNFFNC-TTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEI-RDDKTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSL-VDDRVNILGFSIFNQSHAFFQEFSQSLN------------QSWQENCDHVPFTGPALSS--ALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALKILQFTRNGFQQIGQWHVAEGLSMDSRLYASNISDSLFNTTLVVTTILENPYLMLKGNHQEMEGNDR----YEGFCVDMLKELAEILRFNYKIRLVGDGVYGV-------PEANGTWTGMVGELIARK 493          

HSP 3 Score: 122.479 bits (306), Expect = 1.749e-27
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I  +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  489 LIARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRRPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Mouse
Match: Grik5 (glutamate receptor, ionotropic, kainate 5 (gamma 2) [Source:MGI Symbol;Acc:MGI:95818])

HSP 1 Score: 211.46 bits (537), Expect = 2.507e-55
Identity = 101/194 (52.06%), Postives = 141/194 (72.68%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMK-KNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +P++S +DLA QTNI+YGT+  G++ TFF+ +   TYQ+M+ +M+ K   VFV STE+G+ +V NS  +YAFLLES MN+++ + +C+L +IG LLD+KGYGIG P GSP RD +T +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  607 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPVESADDLADQTNIEYGTIHAGSTMTFFQNSRYQTYQRMWNYMQSKQPSVFVKSTEEGIARVLNS--RYAFLLESTMNEYHRRLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQENNRLEILKRKWWEGGRCPKEEDHRAKG 798          

HSP 2 Score: 122.865 bits (307), Expect = 1.384e-27
Identity = 58/120 (48.33%), Postives = 81/120 (67.50%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSE--LDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC       L+NQ+ L NSLWF VG  ++QG
Sbjct:  488 LINRKADLAVAAFTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRARPHILENQYTLGNSLWFPVGGFMQQG 607          

HSP 3 Score: 120.168 bits (300), Expect = 9.439e-27
Identity = 112/442 (25.34%), Postives = 198/442 (44.80%), Query Frame = 1
Query:  313 CNLIRHGLVAMISPIAEVPSRH-ISSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAI----LFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            C ++  G+V+++ P +   S   +S +C +  IPH+      PE   R        + L P+ +  +  + + +     +    LI +   C+L+ E L+  F  S+  +   +  D +    LL+E+ +  K    ++D         L  AS   MT   + +I T  D  ++ +    + +  NI  FS+  T H       ++                 S + + E +    PA+    +FD V+ +   +  L +S+ + V+P+ C S + W  G+SL+N +   E   G+TG++ FN   QR NY L I+E +  G ++ G W  +R + +        +      + L VTTI+  P+V  +   P+ Q    N E + GFC+D+LRE+   L   + + LV+D  YG        P+GS  W GM+GELI ++
Sbjct:   83 CQILPKGVVSVLGPSSSPASASTVSHICGEKEIPHIKVG---PEETPRLQYLRFASVSLYPSNEDVSLAVSRILKSFN-YPSASLICAKAECLLRLEELVRGFLISKETLSVRMLDDSRDPTPLLKEI-RDDKVSTIIIDANASISHLVLRKASELGMTSAFYKYILTTMDFPILHLDGI-VEDSSNILGFSMFNTSHPFYPEFVRSL--------------NMSWRENCEASTYPGPALSAALMFDAVHVVVSAVRELNRSQEIGVKPLACTSANIWPHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLRILEKSRQGHREIGVWYSNRTLAMNATTLDINLSQTLANKTLVVTTILENPYVMRR---PNFQALSGN-ERFEGFCVDMLRELAELLRFRYRLRLVEDGLYGAP-----EPNGS--WTGMVGELINRK 492          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Mouse
Match: Grik2 (glutamate receptor, ionotropic, kainate 2 (beta 2) [Source:MGI Symbol;Acc:MGI:95815])

HSP 1 Score: 202.601 bits (514), Expect = 7.476e-53
Identity = 102/196 (52.04%), Postives = 139/196 (70.92%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNRE-VFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSV 4036
            G E  P+A STR+V G WWFFTLIIIS+YTANLAAFLTVE++  PI S +DLA+QT I+YG ++ G + TFF+K+ I TY KM+ FM   R+ V V S E+G+ +V  S+  YAFL+ES   +F +QR+C+L +IG L+DSKGYG+G P GSP RD +T +IL+LQ+  K+  +  KWW      E  +SKE+ ++
Sbjct:  623 GSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVEDGATMTFFKKSKISTYDKMWAFMSSRRQSVLVKSNEEGIQRVLTSD--YAFLMESTTIEFVTQRNCNLTQIGGLIDSKGYGVGTPMGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEE-ESKEASAL 815          

HSP 2 Score: 156.762 bits (395), Expect = 3.197e-38
Identity = 111/456 (24.34%), Postives = 223/456 (48.90%), Query Frame = 1
Query:  244 NYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLL---NQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNE-NAQRKNYNLSIMELTENGFQKGGFWNDDRFVVL--PKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELI 1593
            N      T+ ++  +SF A +K C+ +  G+ A+  P     +  + S+C+   +PH+ T  +   S ++    + L P+    ++ I   + Q   W+ + +++     +++ + L+   +++  R K+RQ +  D K  K LL+E+ + K++ + + D  +      L  A    M    + +IFT  D   +D+  ++ +  VN+T F I +       T+    +EK     ++         ++    +  A+++D V+ ++  + +  +   + V  ++C  +  WR G+  ++ +  A +  G+TG+I FN+ N  R +++L ++ L E G +K G W+    + +   ++ +P+ +      R L VTTI+ +P+V +K+   D+  Y    + + G+CIDLLRE+   LG  + I LV+D KYG         D + +WNGM+ ELI
Sbjct:   73 NTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDSFYVSLYPDFSSLSRAILDLV-QFFKWKTVTVVYDDSTGLIRLQELIKAPSRYNLRLKIRQ-LPADTKDAKPLLKEMKRGKEF-HVIFDCSHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSG-VNMTGFRILN----TENTQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDAALMYDAVHVVSVAVQQFPQ---MTVSSLQCNRHKPWRFGTRFMSLIKEA-HWEGLTGRITFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPSSGLNMTESQKGKPANITDSLSNRSLIVTTILEEPYVLFKKS--DKPLYGN--DRFEGYCIDLLRELSTILGFTYEIRLVEDGKYG------AQDDVNGQWNGMVRELI 506          

HSP 3 Score: 144.821 bits (364), Expect = 1.838e-34
Identity = 64/119 (53.78%), Postives = 93/119 (78.15%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSE-LDNQFNLLNSLWFTVGALVRQG 2273
             I+ +A+LA+AP+ I+Y REKV+DF+ PFM+LG+SIL+ KP    PG+FSFL+P+S  +W+YI+ AY+GVS ++F I+  +PYEW   HPC+ +S+ ++N F LLNS WF VGAL++QG
Sbjct:  505 LIDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYILLAYLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQG 623          
BLAST of Glutamate receptor, ionotropic kainate vs. UniProt/SwissProt
Match: sp|Q91755|GRIK2_XENLA (Glutamate receptor ionotropic, kainate 2 (Fragment) OS=Xenopus laevis OX=8355 GN=grik2 PE=2 SV=1)

HSP 1 Score: 201.06 bits (510), Expect = 2.407e-56
Identity = 98/194 (50.52%), Postives = 139/194 (71.65%), Query Frame = 2
Query: 3458 EFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMK-KNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSV 4036
            E  P+A STR+V G WWFFTLIIIS+YTANLAAFLTVE++  PI S +DLA+QT I+YG ++ G + TFF+K+ IPTY+KM+ FM  +++ V V + E+G+ +   S+  YAFL+ES   +F +QR+C+L +IG L+DSKGYG+G P GSP RD +T +IL+LQ+   +  +  KWW      E  +SKE+ ++
Sbjct:    2 ELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGAVQDGATMTFFKKSRIPTYEKMWAFMNSRSQSVLVKNNEEGIQRALTSD--YAFLMESTTIEFVTQRNCNLTQIGGLIDSKGYGVGTPMGSPYRDKITIAILQLQEEGVLHMMKEKWWRGNGCPEE-ESKEASAL 192          
BLAST of Glutamate receptor, ionotropic kainate vs. UniProt/SwissProt
Match: sp|Q01812|GRIK4_RAT (Glutamate receptor ionotropic, kainate 4 OS=Rattus norvegicus OX=10116 GN=Grik4 PE=1 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 8.768e-55
Identity = 105/194 (54.12%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 799          

HSP 2 Score: 150.984 bits (380), Expect = 1.895e-35
Identity = 134/505 (26.53%), Postives = 227/505 (44.95%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSCRFPESLDRYPMD----IQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKKCD----IEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            S   L I  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G+VA++ P +   S  I  ++C +  +PH   +   PE   R+ +     + L P+    +  +   +          LI +   C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    ++          L+ A+   M    +++IFT+ + SL  + +  ++++VNI  FSI    H+  +  +++              +   + CD       A  S  A+LFD V  +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E L G+TG I FN   QR NY L I++ T NGF++ G W+    + +  R+  S +        L VTTI+  P++  K    D +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI ++
Sbjct:   21 SPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVA---PEEFVRFQLQRFTTLNLHPSNTDISVAVAGILNFFNC-TTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEI-RDDKTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSL-VDDRVNILGFSIFNQSHAFFQEFSQSLN------------QSWQENCDHVPFTGPALSS--ALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALKILQFTRNGFRQIGQWHVAEGLSMDSRLYASNISDSLFNTTLVVTTILENPYLMLKGNHQDMEGNDR----YEGFCVDMLKELAEILRFNYKIRLVGDGVYGV-------PEANGTWTGMVGELIARK 493          

HSP 3 Score: 119.783 bits (299), Expect = 9.259e-26
Identity = 57/120 (47.50%), Postives = 82/120 (68.33%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I  +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FS L P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  489 LIARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRRPGYFSSLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. UniProt/SwissProt
Match: sp|Q8BMF5|GRIK4_MOUSE (Glutamate receptor ionotropic, kainate 4 OS=Mus musculus OX=10090 GN=Grik4 PE=2 SV=2)

HSP 1 Score: 212.231 bits (539), Expect = 8.768e-55
Identity = 105/194 (54.12%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 799          

HSP 2 Score: 151.369 bits (381), Expect = 1.709e-35
Identity = 134/505 (26.53%), Postives = 227/505 (44.95%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSCRFPESLDRYPMD----IQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKKCD----IEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            S   L I  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G+VA++ P +   S  I  ++C +  +PH   +   PE   R+ +     + L P+    +  +   +          LI +   C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    ++          L+ A+   M    +++IFT+ + SL  + +  ++++VNI  FSI    H+  +  +++              +   + CD       A  S  A+LFD V  +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E L G+TG I FN   QR NY L I++ T NGFQ+ G W+    + +  R+  S +        L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI ++
Sbjct:   21 SPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVA---PEEFVRFQLQRFTTLNLHPSNTDISVAVAGILNFFNC-TTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEI-RDDKTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSL-VDDRVNILGFSIFNQSHAFFQEFSQSLN------------QSWQENCDHVPFTGPALSS--ALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALKILQFTRNGFQQIGQWHVAEGLSMDSRLYASNISDSLFNTTLVVTTILENPYLMLKGNHQEMEGNDR----YEGFCVDMLKELAEILRFNYKIRLVGDGVYGV-------PEANGTWTGMVGELIARK 493          

HSP 3 Score: 122.479 bits (306), Expect = 1.224e-26
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I  +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  489 LIARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRRPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. UniProt/SwissProt
Match: sp|Q16099|GRIK4_HUMAN (Glutamate receptor ionotropic, kainate 4 OS=Homo sapiens OX=9606 GN=GRIK4 PE=2 SV=2)

HSP 1 Score: 212.231 bits (539), Expect = 1.011e-54
Identity = 105/194 (54.12%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMDVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 799          

HSP 2 Score: 143.665 bits (361), Expect = 3.303e-33
Identity = 129/503 (25.65%), Postives = 223/503 (44.33%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSCRFPESLDRYPMD----IQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAI----LFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            S   L I  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G+VA++ P +   S  I  ++C +  +PH   +   PE   ++       + L P+    +  +   +          LI +   C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    ++          L+ A+   M    +++IFT+ + SL  + +  ++++VNI  FSI + S                +   +    S + + +    + PA+    LFD V  +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E L G+TG I FN   QR NY L I++ T NGF++ G W+    + +   +  S +        L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI ++
Sbjct:   21 SPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVA---PEEFVKFQFQRFTTLNLHPSNTDISVAVAGILNFFNC-TTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEI-RDDKTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSL-VDDRVNILGFSIFNQS------------HAFFQEFAQSLNQSWQENCDHVPFTGPALSSALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALKILQFTRNGFRQIGQWHVAEGLSMDSHLYASNISDTLFNTTLVVTTILENPYLMLKGNHQEMEGNDR----YEGFCVDMLKELAEILRFNYKIRLVGDGVYGV-------PEANGTWTGMVGELIARK 493          

HSP 3 Score: 123.25 bits (308), Expect = 8.190e-27
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I  +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  489 LIARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. UniProt/SwissProt
Match: sp|Q5IS46|GRIK4_PANTR (Glutamate receptor ionotropic, kainate 4 OS=Pan troglodytes OX=9598 GN=GRIK4 PE=2 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 1.047e-54
Identity = 105/194 (54.12%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT I+YGT+ GG+S TFF+ +   TYQ+M+ +M  K   VFV STE+G+ +V NSN  YAFLLES MN++Y QR+C+L +IG LLD+KGYGIG P GS  RD    +IL+LQ++ ++  L  KWW      +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMDVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNSN--YAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQLQENNRLEILKRKWWEGGKCPKEEDHRAKG 799          

HSP 2 Score: 145.976 bits (367), Expect = 6.510e-34
Identity = 130/503 (25.84%), Postives = 224/503 (44.53%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHIS-SLCSKFHIPHLVTSCRFPESLDRYPMD----IQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAI----LFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            S   L I  ILD   + SR     I+LA    N+   R   A  +V    L  ++ +      C ++  G+VA++ P +   S  I  ++C +  +PH   +   PE   ++       + L P+    +  +   +          LI +   C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    ++          L+ A+   M    +++IFT+ + SL  + +  ++++VNI  FSI + S                +   +    S + + +    + PA+    LFD V  +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E L G+TG I FN   QR NY L I++ T NGF++ G W+    + +  R+  S +        L VTTI+  P++  K    + +  D     + GFC+D+L+E+   L  N+ I LV D  YG        P+ +  W GM+GELI ++
Sbjct:   21 SPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFELLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVA---PEEFVKFQFQRFTTLNLHPSNTDISVAVAGILNFFNC-TTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEI-RDDKTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSL-VDDRVNILGFSIFNQS------------HAFFQEFAQSLNQSWQENCDHVPFTGPALSSALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALKILQFTRNGFRQIGQWHVAEGLSMDSRLYASNISDTLFNTTLVVTTILENPYLMLKGNHQEMEGNDR----YEGFCVDMLKELAEILRFNYKIRLVGDGVYGV-------PEANGTWTGMVGELIARK 493          

HSP 3 Score: 123.25 bits (308), Expect = 8.694e-27
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDN--SELDNQFNLLNSLWFTVGALVRQG 2273
             I  +A+LA+A +TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NSLWF VG  ++QG
Sbjct:  489 LIARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. TrEMBL
Match: A0A4S2LMY3 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007409 PE=3 SV=1)

HSP 1 Score: 280.026 bits (715), Expect = 8.951e-74
Identity = 170/506 (33.60%), Postives = 275/506 (54.35%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRKVISLAVQNANKELARF----NYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTK---TYE--NVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKC-KSNDTWRLGSSLINAM--TAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWN-DDRFVVLPKRIE-----PSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITK 1599
            +K ++ IGVIL   D+        +V  AN+ LA+      +  +   E +  +N F A ++ C L+ +G+VA+  P     S+ + SL ++F IP++ T      +   Y   + + P    +   +Y+Y+TQVE WR M +IH+    +LKF  L+N FE   K++ W +      K ++ EL  S + + FL+DIP WQ  DFL+MA VHNMT PQFS+ FTDWD  L+D+ AFK++    I T SI   S +++  +    Y+  +V   +   +E      K  I     ++  IL+D+   L  G++ + ++RPL+ + + C +    W LG SLINAM   + + + GITG+  F+ + QR  ++L+ +ELTE GF   G WN D+R  V+ K I+     PS   + ++V  L VTT    PF+     +    N   + +AW G+C +LL  +  ++G N+TI + KD++YG A   +T P+G+  +NG++G+LI K
Sbjct:   59 AKPVIYIGVILAQGDEDMEAAFRTSVDAANQVLAQSVNLRTHQLEPIIEVVSRDNVFLATQQACKLMTNGIVALFGPNVRALSQALLSLANRFGIPYVETYWNHYTTPGTY--SVSMHPKVHSFGLGLYEYLTQVEKWRRMNIIHNQPEKLLKFTTLVNSFEGTAKVKTW-NSGSASAKSMMAELSSSSE-KTFLIDIPAWQARDFLVMAFVHNMTGPQFSYFFTDWDVGLLDLTAFKVDAGAQIKTISILSKSGIKKAMQNPGVYQASSVPLPIPQTIESNLGPPKLGITGVLNTQFGILYDSFLLLAHGLVTVTRTRPLLPQKLSCERPPGLWPLGLSLINAMKSVSPDVVGGITGQFRFDRDGQRTMFDLTTLELTEGGFTPSGVWNIDERMKVVKKIIKRPNKHPS--NLNDVV--LSVTTKADPPFMISISSD-AYGNPLPHPDAWTGYCKELLDALAAEVGFNYTIQIPKDDQYGSA---KTLPNGTVVYNGIMGQLIRK 552          

HSP 2 Score: 263.077 bits (671), Expect = 3.186e-68
Identity = 160/329 (48.63%), Postives = 210/329 (63.83%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCD-SKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKK---PLHPDLTNSKSRERLNLLDHQSGNV--NDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSP 4420
            G+E  PQA+STR+++G WWFF LIII+TYTANLAAFLTVE    PI+SVEDLA Q  IKYGTL+ G+S  FF    IP ++KM +FM K  +V    T  G+ +VR   G YAF+LES+ N++Y QRDCSLM++G LLDSKGYGIGFP GS  RD ++K+ILKLQ +Q +ANL  KWWNE NITEPC   KE    P+ L V Q+GG+FI+L+ GF+   IVSIIE  +   ++K   ++   K + +EL+FA R +++SK+    +H  L  S+S  +L      S NV  N   L  + NM       ES   G +R  +      P
Sbjct:  667 GVELAPQATSTRLLTGVWWFFGLIIIATYTANLAAFLTVENTKSPIESVEDLANQEKIKYGTLRSGSSRDFFRTTTIPVFKKMGEFMDKYPDVTTPDTRTGIERVR--AGGYAFILESSWNEYYVQRDCSLMQVGALLDSKGYGIGFPQGSVWRDPVSKAILKLQNNQVLANLKRKWWNEYNITEPCSLLKERNKAPSPLGVEQVGGVFIMLLVGFLLGFIVSIIEFIIATRDQK---RVTVLKGMWRELKFASRCLTTSKRLKSEVH-KLPRSRSNIKLVTSIPSSMNVLDNRTPLPPAPNM-------ESKVDGQSRDPLLVPSSGP 982          

HSP 3 Score: 128.642 bits (322), Expect = 5.932e-26
Identity = 56/119 (47.06%), Postives = 87/119 (73.11%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSEL-DNQFNLLNSLWFTVGALVRQG 2273
             I  + ++A+A  TI+YEREK+VDF+TP+M++G SILF +PK + P LFSFL P++  VW+Y+++ Y+ V   +F  + ++PYEW   HPCD++S++  NQF LLNS ++ + AL+ QG
Sbjct:  549 LIRKEVDMAVAGFTITYEREKLVDFSTPWMTIGGSILFTRPKSQKPSLFSFLQPLAPKVWLYVVAIYLAVCLCMFAAARLSPYEWNAPHPCDEDSDVTKNQFTLLNSFYYYISALLNQG 667          
BLAST of Glutamate receptor, ionotropic kainate vs. TrEMBL
Match: A0A4E0RD83 (Glutamate receptor ionotropic kainate 3 OS=Fasciola hepatica OX=6192 GN=D915_002434 PE=3 SV=1)

HSP 1 Score: 279.641 bits (714), Expect = 1.681e-73
Identity = 168/508 (33.07%), Postives = 269/508 (52.95%), Query Frame = 1
Query:  139 KKLLNIGVILDASDDISRKVISLAVQNANKELARFNYAFQVKT-----ETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDH-SIMERMTKTYENVEKLLESIVEMEKTSKKC--DIEKAFMS-KP------AILFDTVNFLTKGIIRLLKSRPLIVRPMKC-KSNDTWRLGSSLINAM--TAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDD---RFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITK 1599
            K ++ IGV+    D+         +Q AN+ L R N A Q+       E +   N F A ++ C L+  G+VA+  P     S+ + SL ++F +P++ T      +LD Y +   + P  D + + +Y+Y+T+VE WR + +IH     +LK+  L+N+FE   K++ W  +     + +L E+    + R FLVD+P WQ+ DFL+MA  HNMT  Q+++ FTDWD + +D+ AFK++    I TFSI     I E + +   N  +          TS+     +E+ F+   P       +L+D++  L  G++ L K+R L+ + + C +    W LG SLINAM  T  E + G TG+  F+   QR  ++++ ++LTE GF   G WN D   + V  P +  P  + I N+   LKVTT    PF+  K+ +   +   T  +AW G+CIDLL EI   +G N+T+HL  D  YG +      P+G+E ++G++GELI +
Sbjct:   76 KSVIYIGVLFMQGDEALESAFRTGIQAANQVL-RSNEALQLHQLEPLIEVVPKENMFVATQQACKLLERGIVALYGPRNAAFSKALLSLANRFGVPYVETQWNVSPTLDAYALS--MYPKADTFGEGLYEYLTRVEKWRHVTIIHEQPESLLKYVSLINKFEDGLKVKSWTANS-AATRAMLAEISTGNE-RNFLVDVPTWQVRDFLVMAYAHNMTGTQYTYFFTDWDVNFMDLTAFKVSMGAQIKTFSILSKVGITESLQRGLINARQ-----ANHPGTSRTTGRQMERPFLGLGPEMNTHFGLLYDSILLLAHGLVTLTKTRSLLPQKLSCERPPGLWPLGLSLINAMKSTRPEMVRGYTGQFRFDREGQRTLFDITTLDLTEGGFVPTGIWNVDEQMKIVTRPPKKLPHKM-IKNMPIVLKVTTKQDPPFMIIKKTDAYGKPLITP-DAWQGYCIDLLDEISKKVGFNYTVHLTPDGNYGSST---ILPNGTEIYDGIMGELIRR 568          

HSP 2 Score: 259.61 bits (662), Expect = 4.419e-67
Identity = 163/364 (44.78%), Postives = 222/364 (60.99%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCD-SKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHPDLTN-SKSRERLNLLDH-QSGNVNDVSLEASRNMSNFTSKSESHQSG---------LNRRQIFTDI-----DSPCLDRSLGFDSSHDGLS-SIPNSKP 4489
            G E  PQA+STR+++G WWFF LIII+TYTANLAAFLTVE    PI+S EDLA Q  IKYGT+  G+S  FF+   + T++KM  FM K  E   D TE GV +V+   G YAF+LESA N++Y QRDCSLM++G LLDSKGYGIGFP GSP RD ++ +IL+LQ  Q++  L  KWWNE NITEPC+  +ES   P+ L V QIGG+FI+L+ GF+   +VSI+E  +   +K     +   K + +EL FA+R +++SK+ L  D+    +SR  + L+   QSG            ++ F    + H +G         +  RQ+ T I     D P    S+ F++   G+  S+P S P
Sbjct:  683 GTELAPQATSTRLLTGVWWFFALIIIATYTANLAAFLTVENTKSPIESAEDLANQDKIKYGTISSGSSRDFFKTTTLSTFKKMGAFMDKYPEYLADRTEIGVERVK--QGNYAFILESAWNEYYVQRDCSLMQVGALLDSKGYGIGFPQGSPWRDRVSSAILQLQNDQQLDKLKRKWWNEYNITEPCNLLRESSKAPSPLGVEQIGGVFIMLLVGFVVGFLVSIVEFVIATRDKNRSEML---KAMWRELTFAVRCLTTSKR-LKSDVKKMPRSRSNIKLISSVQSGMC-----AMDPKLAFFPPNFDPHVTGDSADPLLSNVPGRQVPTYIGTAQPDIPSSFGSVDFEAGPPGVEHSVPGSGP 1035          

HSP 3 Score: 125.176 bits (313), Expect = 6.521e-25
Identity = 56/119 (47.06%), Postives = 82/119 (68.91%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSEL-DNQFNLLNSLWFTVGALVRQG 2273
             I  + ++A+A  TI+YEREKVVDF+TP+M++G SI+F +PK + P LFSFL P++  VW Y+++ Y  V   +F  S ++PYEW   H CDD  ++  NQF LLNS ++ + AL+ QG
Sbjct:  565 LIRREVDMAVAAFTITYEREKVVDFSTPWMTIGGSIMFTRPKSQKPSLFSFLQPLAPQVWFYVLAIYFAVCVCMFISSRLSPYEWNSPHACDDGGDMTKNQFTLLNSFYYHISALLNQG 683          
BLAST of Glutamate receptor, ionotropic kainate vs. TrEMBL
Match: A0A074Z9I4 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_08425 PE=3 SV=1)

HSP 1 Score: 277.33 bits (708), Expect = 6.977e-73
Identity = 168/506 (33.20%), Postives = 275/506 (54.35%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRKVISLAVQNANKELARF----NYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTK---TYE--NVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKC-KSNDTWRLGSSLINAM--TAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWN-DDRFVVLPKRIE-----PSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITK 1599
            +K ++ IGVIL   D+        +V +AN+ LA+      +  +   E +  +N F A ++ C L+ +G+VA+  P     S+ + SL ++F IP++ T      +   Y   + + P    +   +Y+Y+TQVE WR M +IH+    +LKF  L+N FE   K++ W        K ++ EL  S + + FL+DIP WQ  DFL+MA VHNMT PQFS+ FTDWD  L+D+ AFK++    I T SI   + +++  +    Y+  +V   +   ++      K  I     ++  IL+D+   L  G++ + ++RPL+ + + C +    W LG SLINAM   + + + GITG+  F+ + QR  ++L+ +ELTE GF   G WN D+R  V+ K I+     PS   + ++V  L VTT    PF+     +    N   + +AW G+C +LL  +  ++G N+TI + KD++YG A   +T P+G+  +NG++G+LI K
Sbjct:   59 AKPVIYIGVILAQGDEDMEAAFRTSVDSANQVLAQSVNLRTHQLEPIIEVVSRDNVFLATQQACKLMTNGIVALFGPNVRALSQALLSLANRFGIPYVETYWNHYATPGTY--SVSMHPKVHSFGLGLYEYLTQVEKWRRMNIIHNQPEKLLKFTTLVNSFEGTAKVKTWSSGSASA-KSMMAELSTSSE-KTFLIDIPAWQARDFLVMAFVHNMTGPQFSYFFTDWDVGLLDLTAFKVDAGAQIKTISILSKTGIKKAMQNPGVYQASSVPLPIPQTIDSNLGPPKQGITGVLNTQFGILYDSFLLLAHGLVTVTRTRPLLPQKLSCERPPGLWPLGLSLINAMKSVSPDVVGGITGQFRFDRDGQRTMFDLTTLELTEGGFTPSGVWNIDERMKVVKKIIKRPNKHPS--SMNDVV--LSVTTKADPPFMISISSD-AYGNPLPHPDAWTGYCKELLDALAAEVGFNYTIQIPKDDQYGSA---KTLPNGTVVYNGIMGQLIRK 552          

HSP 2 Score: 266.159 bits (679), Expect = 2.980e-69
Identity = 161/329 (48.94%), Postives = 212/329 (64.44%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCD-SKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKK---PLHPDLTNSKSRERLNLLDHQSGNV--NDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSP 4420
            G+E  PQA+STR+++G WWFF LIII+TYTANLAAFLTVE    PI+SVEDLA Q  IKYGTL+ G+S  FF    IP ++KM +FM K  +V    T  G+ +VR   G YAF+LES+ N++Y QRDCSLM++G LLDSKGYGIGFP GS  RD ++K+ILKLQ +Q +ANL  KWWNE NITEPC   KE    P+ L V Q+GG+FI+L+ GF+   IVSIIE  +   ++K   ++   K + +EL+FA R +++SK+    +H  L  S+S  +L      S NV  N +SL  + NM       ES   G +R  +      P
Sbjct:  667 GVELAPQATSTRLLTGVWWFFGLIIIATYTANLAAFLTVENTKSPIESVEDLANQEKIKYGTLRSGSSRDFFRTTTIPVFKKMGEFMDKYPDVTTPDTRTGIERVR--AGGYAFILESSWNEYYVQRDCSLMQVGALLDSKGYGIGFPQGSVWRDPVSKAILKLQNNQVLANLKRKWWNEYNITEPCSLLKERNKAPSPLGVEQVGGVFIMLLVGFLLGFIVSIIEFVIATRDQK---RVTVLKGMWRELKFASRCLTTSKRLKSEVH-KLPRSRSNIKLVTSIPSSMNVLDNRISLPPAPNM-------ESKVDGQSRDPLIVPSSGP 982          

HSP 3 Score: 128.642 bits (322), Expect = 5.541e-26
Identity = 56/119 (47.06%), Postives = 87/119 (73.11%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSEL-DNQFNLLNSLWFTVGALVRQG 2273
             I  + ++A+A  TI+YEREK+VDF+TP+M++G SILF +PK + P LFSFL P++  VW+Y+++ Y+ V   +F  + ++PYEW   HPCD++S++  NQF LLNS ++ + AL+ QG
Sbjct:  549 LIRKEVDMAVAGFTITYEREKLVDFSTPWMTIGGSILFTRPKSQKPSLFSFLQPLAPKVWLYVVAIYLAVCLCMFAAARLSPYEWNAPHPCDEDSDVTKNQFTLLNSFYYYISALLNQG 667          
BLAST of Glutamate receptor, ionotropic kainate vs. TrEMBL
Match: A0A419PJX9 (Glutamate receptor ionotropic OS=Clonorchis sinensis OX=79923 GN=Grik3 PE=3 SV=1)

HSP 1 Score: 276.559 bits (706), Expect = 1.732e-72
Identity = 168/506 (33.20%), Postives = 273/506 (53.95%), Query Frame = 1
Query:  136 SKKLLNIGVILDASDDISRKVISLAVQNANKELARF----NYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTK---TYENVEKLL--ESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKC-KSNDTWRLGSSLINAM--TAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWN-DDRFVVLPKRIE-----PSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITK 1599
            +K ++ IGVIL   ++        +V  AN+ LA+      +  +   E +  +N F A ++ C L+ +G+VA+  P     S+ + SL ++F IP++ T      +   Y   + + P    +   +Y+Y+TQVE WR M +IH+    +LKF  L+N FE   K++ W +      K ++ EL  S + + FL+DIP WQ  DFL+MA VHNMT PQFS+ FTDWD  L+D+ AFK++    I T SI   + +++  +    Y+     L     +E      K  I     ++  IL+D+   L  G++ + ++RPL+ + + C +    W LG SLINAM   + + + GITG+  F+ + QR  ++L+ +ELTE GF   G WN D+R  V+ K I+     PS   + ++V  L VTT    PF+     +    N   + +AW G+C +LL  +  ++G N+TI + KD++YG A   +T P+G+  +NG++G+LI K
Sbjct:   83 AKPVIYIGVILAQGEEEMEAAFRTSVDAANQVLAQSVNLRTHQLEPIIEVVSRDNVFLATQQACKLMTNGIVALFGPNVRALSQALLSLANRFGIPYVETYWNHYATPGTY--SVSMHPKVHSFGLGLYEYLTQVEKWRRMNIIHNQPEKLLKFTTLVNSFEGTAKVKTW-NSGSASAKSMMAELSTSSE-KTFLIDIPAWQARDFLVMAFVHNMTGPQFSYFFTDWDVGLLDLTAFKVDAGAQIKTISILSKTGIKKAMQNPGVYQASSAPLPIPQTIESNLGPPKLGITGVLNTQFGILYDSFLLLAHGLVTVTRTRPLLPQKLSCERPPGLWPLGLSLINAMKSVSPDVVGGITGQFRFDRDGQRTMFDLTTLELTEGGFTPSGVWNIDERMKVVKKIIKRPNKHPS--SMNDVV--LSVTTKADPPFMISISSD-AYGNPLPHPDAWTGYCKELLDALAAEVGFNYTIQIPKDDQYGSA---KTLPNGTVVYNGIMGQLIRK 576          

HSP 2 Score: 265.774 bits (678), Expect = 5.063e-69
Identity = 161/329 (48.94%), Postives = 212/329 (64.44%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCD-SKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKK---PLHPDLTNSKSRERLNLLDHQSGNV--NDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSP 4420
            G+E  PQA+STR+++G WWFF LIII+TYTANLAAFLTVE    PI+SVEDLA Q  IKYGTL+ G+S  FF    IP ++KM +FM K  +V    T  G+ +VR   G YAF+LES+ N++Y QRDCSLM++G LLDSKGYGIGFP GS  RD ++K+ILKLQ +Q +ANL  KWWNE NITEPC   KE    P+ L V Q+GG+FI+L+ GF+   IVSIIE  +   ++K   ++   K + +EL+FA R +++SK+    +H  L  S+S  +L      S NV  N +SL  + NM       ES   G +R  +      P
Sbjct:  691 GVELAPQATSTRLLTGVWWFFGLIIIATYTANLAAFLTVENTKSPIESVEDLANQEKIKYGTLRSGSSRDFFRTTTIPVFKKMGEFMDKYPDVTTPDTRTGIERVR--AGGYAFILESSWNEYYVQRDCSLMQVGALLDSKGYGIGFPQGSVWRDPVSKAILKLQNNQVLANLKRKWWNEYNITEPCSLLKERNKAPSPLGVEQVGGVFIMLLVGFLLGFIVSIIEFVIATRDQK---RVTVLKGMWRELKFASRCLTTSKRLKSEVH-KLPRSRSNIKLVTSIPSSMNVLDNRISLPPAPNM-------ESKVDGQSRDPLIVPSSGP 1006          

HSP 3 Score: 128.642 bits (322), Expect = 5.263e-26
Identity = 56/119 (47.06%), Postives = 87/119 (73.11%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSEL-DNQFNLLNSLWFTVGALVRQG 2273
             I  + ++A+A  TI+YEREK+VDF+TP+M++G SILF +PK + P LFSFL P++  VW+Y+++ Y+ V   +F  + ++PYEW   HPCD++S++  NQF LLNS ++ + AL+ QG
Sbjct:  573 LIRKEVDMAVAGFTITYEREKLVDFSTPWMTIGGSILFTRPKSQKPSLFSFLQPLAPKVWLYVVAIYLAVCLCMFAAARLSPYEWNAPHPCDEDSDVTKNQFTLLNSFYYYISALLNQG 691          
BLAST of Glutamate receptor, ionotropic kainate vs. TrEMBL
Match: A0A4Z2DUZ1 (Glutamate receptor ionotropic, kainate 3 OS=Schistosoma japonicum OX=6182 GN=EWB00_004199 PE=3 SV=1)

HSP 1 Score: 269.24 bits (687), Expect = 1.340e-70
Identity = 165/527 (31.31%), Postives = 281/527 (53.32%), Query Frame = 1
Query:   73 MLIFGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRKVISLAVQNANKELARF---NYAFQVKT--ETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQW-VHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESI----VEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRP---MKCKSNDTWRLGSSLINAMTAAE--NLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRP--LKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            ML +   I F+ +  I ++      L IG +++  D         ++Q ANK L +    N+ FQ+    ET+D ++SF A RK C L+   ++A+  P     S  + +LC++F IPHL     + ++   Y ++I   P+   + + +Y Y+ + E W  + +I+S +  +LKF++LL  F+    +R+W V +D    K ++++   ++ +  F+VD+P+W++ +FL +A+ +NMT   +S++FTDWD  LVD  AF+     NITTF+I  H + E    +   V  LL+ I    ++ ++ ++     +   +  A+L+D ++ L  G+  L  SR   + P   + C  N  W  G +LIN + A    +  G+TG   F+ +  R N +L I+EL+ NGFQ+ G+WN D+ +V+ K    +  E  + ++   L++TT   +P++ YK      Q   T+ + W GFCI LL  I  DL   +TI+LV D+ YG    N    DG E W+GM+ EL T+R
Sbjct:    1 MLFYTISIIFIQLSCICSQTYESTQLIIGALIEKDDVAQEICFKKSIQAANKLLQQHDSTNHRFQLIPIIETIDGDDSFEATRKACQLVERQVIAIYGPSTPHASSAVQALCNQFGIPHLQIDWGYHQTSRGYALNIH--PHYLAFGQALYDYVQKAEYWDTVAVIYSREESLLKFDYLLRTFDQPLMLRKWDVSND--NQKYVIKQFKITQTHFRFIVDLPFWEVQEFLALANAYNMTSQYYSYVFTDWDVQLVDPKAFESVQGANITTFTIL-HPMNE---DSGYGVAALLDDIRVTALQDKRFTRSSTSRRISPTHSALLYDGLSLLAMGV--LSSSRSTNILPPVGLSCSVNRVWNPGLTLINDIKAVHPTSFRGLTGPFQFDGHGWRSNVHLIILELSRNGFQEYGYWNMDKGLVVTKNFSLTKQEYQSELKGKVLRITTQEEKPYMMYKGKTLPGQPKSTDPKDWEGFCISLLNYISEDLQFTYTINLVPDSTYG----NMKIVDGVETWDGMVQELRTRR 513          

HSP 2 Score: 225.713 bits (574), Expect = 2.099e-56
Identity = 106/217 (48.85%), Postives = 156/217 (71.89%), Query Frame = 2
Query: 3389 DFVCNNFISXXXXXXIF--IASIGLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGS 4033
            D V NNF     L   F  +   G +  P A+STR+++G WWFFTLI+IS+YTANLAAFLTV +++ PI++ EDLA+QT IKYG+++GG++  FFE++   TY++M++FM   + + +++T + + +V+    +YAFLLES MN++Y+QRDC LM++G LLDSKGYGIG P GS  RD ++++ILKLQKSQ +  L   WW + +I +PCD+  + S
Sbjct:  604 DVVENNFNVSNSLWFTFGSLMQKGSDILPHATSTRIIAGFWWFFTLIVISSYTANLAAFLTVARMVAPIENAEDLAKQTKIKYGSIQGGSTTAFFEESNFSTYKRMWQFMSSQKGLLMNNTLEAIQRVKRE--EYAFLLESTMNEYYTQRDCELMQVGGLLDSKGYGIGLPEGSKYRDPISETILKLQKSQVLEQLKFYWWRKHDIEKPCDTSPTKS 818          

HSP 3 Score: 131.724 bits (330), Expect = 5.957e-27
Identity = 58/115 (50.43%), Postives = 89/115 (77.39%), Query Frame = 3
Query: 1932 QAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCD-DNSELDNQFNLLNSLWFTVGALVRQG 2273
            +A+LA+   TI+Y+RE+V+DFTTPFM LG+SI++ +P+ +   LFSFL+P+S PVW YI++A I VS ++F ++  +PYEW   HPC+ D+  ++N FN+ NSLWFT G+L+++G
Sbjct:  513 RADLAVGSFTITYDRERVIDFTTPFMYLGISIIYKRPEDKESHLFSFLTPLSPPVWGYILAALIMVSLVLFVVARFSPYEWKVKHPCNVDSDVVENNFNVSNSLWFTFGSLMQKG 627          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Cavefish
Match: si:ch211-264f5.8 (si:ch211-264f5.8 [Source:ZFIN;Acc:ZDB-GENE-081104-209])

HSP 1 Score: 211.075 bits (536), Expect = 3.869e-55
Identity = 102/194 (52.58%), Postives = 139/194 (71.65%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+S +DLA QTNI+YGT+ GG++ TFF  +   TYQ+M+ +M  K   VFV STE+G+ +V NS  KYAFLLES MN+++ + +C+L +IG LLD+KGYGIG P GSP RD +T  +L+LQ++ ++  L  +WW      +  D +  G
Sbjct:  571 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESADDLADQTNIQYGTIHGGSTMTFFMNSRYQTYQRMWNYMYSKQPIVFVKSTEEGIARVLNS--KYAFLLESTMNEYHRRHNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLGVLQLQENNRLEILKRRWWEGGECPKEEDHRAKG 762          

HSP 2 Score: 137.887 bits (346), Expect = 2.619e-32
Identity = 117/439 (26.65%), Postives = 207/439 (47.15%), Query Frame = 1
Query:  313 CNLIRHGLVAMISPIAEVPSRH-ISSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKK-CDIEK--AFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            C ++  G+V++I P +   S   +S +C +  IPH+      PE   R P      + L P+ +  +  I   +     +    LI +   C+L+ E L+ +F  SR  +   +  +      LL+E+ +  K    ++D         L  AS   MT   + +I T  D  L+ +    ++++ NI  FS+ ++S               LE I  +  + ++ CD+          A++FD V+ +   +  L +S+ + V+P+ C S+  W+ G+SL+N +   E   G+TG++ FN   QR NY L ++E    G ++ G W  +  + +      + +      + L VTTI+  P+V  KE   + Q+++ N + + GFC+D+LRE+ + L  +F I LV D  YG        P+GS  W GM+GELI ++
Sbjct:   47 CQILPKGVVSVIGPASSPASASTVSHICGEKEIPHIKIG---PEETPRLPYLRFASVTLYPSNEDLSMAIGAILRSFN-YPTASLICAKAECLLRLEELVRRFLISRDTLSVRMLDESLDPTPLLKEI-RDDKVATIIIDANASVSILILKKASELGMTSAFYKYILTTMDFPLLRLDDV-VDDQSNIVGFSMLNNS-----------HPFYLEFIRSLNLSWREGCDLSPYPGPALSSALMFDAVHVVVGAVRELNRSQEIGVKPLSCTSSLIWQHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLCVLEKFRGGHREIGIWYSNNTLAMNSTSLDNNMSHTLANKTLTVTTILENPYVMRKE---NYQDFEGN-DQYEGFCVDMLRELSDILKFSFRIKLVDDGLYGAP-----EPNGS--WTGMVGELINRK 456          

HSP 3 Score: 124.02 bits (310), Expect = 5.776e-28
Identity = 58/120 (48.33%), Postives = 82/120 (68.33%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC  +    L+NQ+ L NSLWF VG  ++QG
Sbjct:  452 LINRKADLAVAGFTITSEREKVIDFSKPFMTLGISILYRVHIGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRERRDLLENQYTLGNSLWFPVGGFMQQG 571          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Cavefish
Match: grik5 (glutamate ionotropic receptor kainate type subunit 5 [Source:NCBI gene;Acc:103024859])

HSP 1 Score: 207.608 bits (527), Expect = 4.301e-54
Identity = 101/194 (52.06%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++  PI+S +DLA QTNI+YGT+ GG++ TFF  +   TYQ+M+ +M  K   VFV STE+G+ +V NS  KYAFLLES MN+++   +C+L +IG LLD+KGYGIG P GSP RD ++ ++L+LQ++ ++  L  +WW      +  D +  G
Sbjct:  621 GSEIMPKALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEAPIESPDDLADQTNIQYGTIHGGSTMTFFMNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVVNS--KYAFLLESTMNEYHRSLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEISLAVLQLQENNRLEILKRRWWEGGQCPKEEDHRAKG 812          

HSP 2 Score: 134.42 bits (337), Expect = 3.601e-31
Identity = 140/540 (25.93%), Postives = 240/540 (44.44%), Query Frame = 1
Query:   55 EFIIL*MLIFGFVIFFLSICPISTKELSK----KLLNIGVILD---ASDDISRKVISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPS-RHISSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLV---DIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKK-CDIEK--AFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFW-NDDRFVVLPKRIEPSVVE-IGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            E   L +L   F I  +++  +    LS+      + +  ILD   A     R  ++LA +N N  +     A   V    L  ++ +      C ++  G+V++I P +   S   +S +C +  IPH+      PE   + P      + L P+ +  +  +   +     +    L+ +   C+L+ E L+ +F  SR  +   +  D      LL+E+ +  K    ++D         L  AS   MT   + +I T  D  L+   DI    ++   NI  FS+  T H                LE I  +  + ++ CDI          A+LFD V+ +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E   G+TG++ FN   QR NY L I+E    G ++ G W +++  V+    ++ +  E + N  + L +TTI+  P+V  K    + Q  + N + + GFC+D+L+E+ + L   F I LV D  YG        P+GS  W GM+GELI ++
Sbjct:    3 ELPALLLLSIHFHILLVTVITMPPSALSQPSVLSSVRMAAILDDHSACGRGERLALALARENINSVMEGPARARVDVDVFELQKDSQYETTDTMCQILPKGVVSVIGPASSPASGSTVSHICGEKEIPHVKIG---PEETPKLPYLRFASVTLYPSNEDLSLAVGAILRSFS-YPSASLVCAKAECLLRLEELVRRFLISRETLSVRMLDDDLDPTSLLKEI-RDDKIATIIIDANASVSYMILKKASELGMTSALYKYILTTMDFPLLWLDDI----VDEHSNIVGFSMFNTTHPFY-------------LEFIRSLNLSWREGCDINPYPGPALSSALLFDAVHVVASAVQELNRSQEIGVKPLSCTSPQIWQHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLHILEKHRYGHKQIGIWYSNNTLVMNSTSLDINASETLAN--KTLIITTILENPYVMRKV---NYQELEGN-DQYEGFCVDMLKELSDILKFTFRIKLVDDGLYGAP-----EPNGS--WTGMVGELINRK 506          

HSP 3 Score: 124.405 bits (311), Expect = 3.802e-28
Identity = 58/120 (48.33%), Postives = 82/120 (68.33%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC  +    L+NQ+ L NSLWF VG  ++QG
Sbjct:  502 LINRKADLAVAAFTITSEREKVIDFSKPFMTLGISILYRVHIGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRERRDMLENQYTLGNSLWFPVGGFMQQG 621          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Cavefish
Match: grik5 (glutamate ionotropic receptor kainate type subunit 5 [Source:NCBI gene;Acc:103024859])

HSP 1 Score: 207.608 bits (527), Expect = 5.760e-54
Identity = 101/194 (52.06%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++  PI+S +DLA QTNI+YGT+ GG++ TFF  +   TYQ+M+ +M  K   VFV STE+G+ +V NS  KYAFLLES MN+++   +C+L +IG LLD+KGYGIG P GSP RD ++ ++L+LQ++ ++  L  +WW      +  D +  G
Sbjct:  621 GSEIMPKALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEAPIESPDDLADQTNIQYGTIHGGSTMTFFMNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVVNS--KYAFLLESTMNEYHRSLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEISLAVLQLQENNRLEILKRRWWEGGQCPKEEDHRAKG 812          

HSP 2 Score: 134.42 bits (337), Expect = 3.818e-31
Identity = 142/542 (26.20%), Postives = 242/542 (44.65%), Query Frame = 1
Query:   55 EFIIL*MLIFGFVIFFLSICPISTKELSK----KLLNIGVILD---ASDDISRKVISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRH-ISSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLV---DIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKK-CDIEK----AFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFW-NDDRFVVLPKRIEPSVVE-IGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            E   L +L   F I  +++  +    LS+      + +  ILD   A     R  ++LA +N N  +     A   V    L  ++ +      C ++  G+V++I P +   S   +S +C +  IPH+      PE   + P      + L P+ +  +  +   +     +    L+ +   C+L+ E L+ +F  SR  +   +  D      LL+E+ +  K    ++D         L  AS   MT   + +I T  D  L+   DI    ++   NI  FS+  T H                LE I  +  + ++ CDI      A  S  A+LFD V+ +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E   G+TG++ FN   QR NY L I+E    G ++ G W +++  V+    ++ +  E + N  + L +TTI+  P+V  K    + Q  + N + + GFC+D+L+E+ + L   F I LV D  YG        P+GS  W GM+GELI ++
Sbjct:    3 ELPALLLLSIHFHILLVTVITMPPSALSQPSVLSSVRMAAILDDHSACGRGERLALALARENINSVMEGPARARVDVDVFELQKDSQYETTDTMCQILPKGVVSVIGPASSPASGSTVSHICGEKEIPHVKIG---PEETPKLPYLRFASVTLYPSNEDLSLAVGAILRSFS-YPSASLVCAKAECLLRLEELVRRFLISRETLSVRMLDDDLDPTSLLKEI-RDDKIATIIIDANASVSYMILKKASELGMTSALYKYILTTMDFPLLWLDDI----VDEHSNIVGFSMFNTTHPFY-------------LEFIRSLNLSWREGCDINPYPGPALSS--ALLFDAVHVVASAVQELNRSQEIGVKPLSCTSPQIWQHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLHILEKHRYGHKQIGIWYSNNTLVMNSTSLDINASETLAN--KTLIITTILENPYVMRKV---NYQELEGN-DQYEGFCVDMLKELSDILKFTFRIKLVDDGLYGAP-----EPNGS--WTGMVGELINRK 506          

HSP 3 Score: 124.02 bits (310), Expect = 5.052e-28
Identity = 58/120 (48.33%), Postives = 82/120 (68.33%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC  +    L+NQ+ L NSLWF VG  ++QG
Sbjct:  502 LINRKADLAVAAFTITSEREKVIDFSKPFMTLGISILYRVHIGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCLRERRDMLENQYTLGNSLWFPVGGFMQQG 621          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Cavefish
Match: GRIK2 (glutamate receptor ionotropic, kainate 2 [Source:NCBI gene;Acc:103047375])

HSP 1 Score: 201.06 bits (510), Expect = 2.521e-52
Identity = 101/196 (51.53%), Postives = 138/196 (70.41%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNRE-VFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSV 4036
            G E  P+A STR+V G WWFFTLIIIS+YTANLAAFLTVE++  PI S +DLA+QT I+YG ++ G++ TFF+K  I TY KM++FM   R  V V S E+G+ +V  S+  YAFL+ES   +F +QR+C+L +IG L+DSK YG+G P GSP RD +T +IL+LQ+  K+  +  KWW      E  +SKE+ ++
Sbjct:  592 GSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGVVEDGSTMTFFKKTKISTYDKMWEFMSSRRHSVMVKSVEEGIQRVLTSD--YAFLMESTTIEFVTQRNCNLTQIGGLIDSKAYGVGTPMGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEE-ESKEASAL 784          

HSP 2 Score: 144.05 bits (362), Expect = 2.688e-34
Identity = 63/119 (52.94%), Postives = 93/119 (78.15%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSE-LDNQFNLLNSLWFTVGALVRQG 2273
             ++ +A+LA+AP+ I+Y REKV+DF+ PFM+LG+SIL+ KP    PG+FSFL+P+S  +W+YI+ AY+GVS ++F I+  +PYEW   HPC+ +S+ ++N F LLNS WF VGAL++QG
Sbjct:  474 LMDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYILLAYLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQG 592          

HSP 3 Score: 130.568 bits (327), Expect = 3.780e-30
Identity = 115/507 (22.68%), Postives = 229/507 (45.17%), Query Frame = 1
Query:  151 NIGVILDASDDISRKVISLAVQ------NANKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLL---NQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFL--------------IMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNE-NAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIV--RPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELI 1593
            N+G I ++ +        LA +      N N+ L   N       + ++ ++SF A RK C+ +  G+ A+  P     +  + S+C+   +PH+ T  +   S +R    + L P+    ++ I   +   + WR + +++     +++ + L+   +++  R K+RQ +  + K  K LL+E+ + K++ + + D  +      L                    +     F+     +  S V+++ F+I N  N    SI +   MER+    +    LL+                 FM+  A L    + +    + + +S  + V  ++C  +  WR G+  +  +  A +  G+TG+I FN+ N  R +++L ++ L E G +K G W+    + + +  +  +  + + +  R L V+TI+ +P+V +K+ +      D     + GFCIDLLRE+ + LG  + + LV+D KYG   E+      + +WNG++ EL+
Sbjct:    2 NLGGIFESIESGPSGAEELAFKFALNTINRNRTLLP-NTTLTYDIQRINIHDSFEASRKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTKWKHQVSDNRDSYYVSLYPDFSSLSRAILDLVHFFK-WRMVTVVYDDSTGLIRLQELIKAPSRYNIRLKIRQ-LPAETKDAKPLLKEMKRGKEF-HVIFDCGHEMAAGILKQNLASHYYYFFFFSHFKSKSFFQDLFALDVEPYRYSGVNMSGFRILNTENSQVSSIIEKWSMERLQAPPKPDSGLLD----------------GFMTTDAALM--YDAVHVVAVAVQQSPQITVSSLQCNRHKPWRFGNRFMTLIKEA-HWDGLTGRINFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPVSGLNMSESQKGKMANVTDSLTNRTLFVSTILEEPYVMFKKSDKPLHGNDR----FEGFCIDLLRELASILGFVYEVRLVEDGKYGAQEES------TGQWNGLVRELM 475          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Cavefish
Match: GRIK2 (glutamate receptor ionotropic, kainate 2 [Source:NCBI gene;Acc:103047375])

HSP 1 Score: 200.675 bits (509), Expect = 2.664e-52
Identity = 101/196 (51.53%), Postives = 138/196 (70.41%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNRE-VFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSV 4036
            G E  P+A STR+V G WWFFTLIIIS+YTANLAAFLTVE++  PI S +DLA+QT I+YG ++ G++ TFF+K  I TY KM++FM   R  V V S E+G+ +V  S+  YAFL+ES   +F +QR+C+L +IG L+DSK YG+G P GSP RD +T +IL+LQ+  K+  +  KWW      E  +SKE+ ++
Sbjct:  624 GSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPIDSADDLAKQTKIEYGVVEDGSTMTFFKKTKISTYDKMWEFMSSRRHSVMVKSVEEGIQRVLTSD--YAFLMESTTIEFVTQRNCNLTQIGGLIDSKAYGVGTPMGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEE-ESKEASAL 816          

HSP 2 Score: 144.05 bits (362), Expect = 2.433e-34
Identity = 111/466 (23.82%), Postives = 221/466 (47.42%), Query Frame = 1
Query:  244 NYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLL---NQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDA----------SLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNE-NAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIV--RPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELI 1593
            N       + ++ ++SF A RK C+ +  G+ A+  P     +  + S+C+   +PH+ T  +   S +R    + L P+    ++ I   +   + WR + +++     +++ + L+   +++  R K+RQ +  + K  K LL+E+ + K++ + + D  +      L  A    M    + +IFT  D           S V+++ F+I N  N    SI +   MER+    +    LL+  +  +                A+++D V+ +      + +S  + V  ++C  +  WR G+  +  +  A +  G+TG+I FN+ N  R +++L ++ L E G +K G W+    + + +  +  +  + + +  R L V+TI+ +P+V +K+ +      D     + GFCIDLLRE+ + LG  + + LV+D KYG   E+      + +WNG++ EL+
Sbjct:   74 NTTLTYDIQRINIHDSFEASRKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTKWKHQVSDNRDSYYVSLYPDFSSLSRAILDLVHFFK-WRMVTVVYDDSTGLIRLQELIKAPSRYNIRLKIRQ-LPAETKDAKPLLKEMKRGKEF-HVIFDCGHEMAAGILKQALAMGMMTEYYHYIFTTLDLFALDVEPYRYSGVNMSGFRILNTENSQVSSIIEKWSMERLQAPPKPDSGLLDGFMTTDA---------------ALMYDAVHVVAV---AVQQSPQITVSSLQCNRHKPWRFGNRFMTLIKEA-HWDGLTGRINFNKTNGLRTDFDLDVISLKEEGLEKIGTWDPVSGLNMSESQKGKMANVTDSLTNRTLFVSTILEEPYVMFKKSDKPLHGNDR----FEGFCIDLLRELASILGFVYEVRLVEDGKYGAQEES------TGQWNGLVRELM 507          

HSP 3 Score: 143.665 bits (361), Expect = 3.021e-34
Identity = 63/119 (52.94%), Postives = 93/119 (78.15%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSE-LDNQFNLLNSLWFTVGALVRQG 2273
             ++ +A+LA+AP+ I+Y REKV+DF+ PFM+LG+SIL+ KP    PG+FSFL+P+S  +W+YI+ AY+GVS ++F I+  +PYEW   HPC+ +S+ ++N F LLNS WF VGAL++QG
Sbjct:  506 LMDHKADLAVAPLAITYVREKVIDFSKPFMTLGISILYRKPNGTNPGVFSFLNPLSPDIWMYILLAYLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTLLNSFWFGVGALMQQG 624          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005084.1 (pep scaffold:Pmarinus_7.0:GL476900:54926:87395:-1 gene:ENSPMAG00000004612.1 transcript:ENSPMAT00000005084.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 195.667 bits (496), Expect = 1.436e-55
Identity = 95/195 (48.72%), Postives = 137/195 (70.26%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSV 4036
            G E  P+A STR+V G WWFFTLIIIS+YTANLAAFLTVE++  PI S +DLA+QT I+YG ++ G++ TFF+K+ I TY+KM+ FM   +   + + E+G+ +V  ++  YA L+ES   ++ +QR+C+L +IG L+D+KGYGIG P GSP RD +T +IL+LQ+  K+  +  KWW      E  DS E+ ++
Sbjct:  123 GSELMPKALSTRIVGGIWWFFTLIIISSYTANLAAFLTVERMESPISSADDLAKQTRIEYGAVRDGSTMTFFKKSKISTYEKMWAFMSSRQTALIKNNEEGIQRVLTTD--YAMLMESTNIEYVTQRNCNLTQIGGLIDNKGYGIGTPLGSPYRDKITIAILQLQEEGKLHMMKEKWWRGNGCPEE-DSNEASAL 314          

HSP 2 Score: 144.821 bits (364), Expect = 5.528e-38
Identity = 63/117 (53.85%), Postives = 92/117 (78.63%), Query Frame = 3
Query: 1926 NTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSE-LDNQFNLLNSLWFTVGALVRQG 2273
            N +A+LA+AP+TI+Y REK +DF+ PFM+LG+SIL+ KP    PG+FSFL+P+S  +W+YI+ AY+GVS ++F I+  +PYEW   HPC+ +S+ ++N F +LNS WF VGAL++QG
Sbjct:    7 NHKADLAVAPLTINYMREKAIDFSKPFMTLGISILYRKPNGTNPGIFSFLNPLSPDIWMYILLAYLGVSCVLFVIARFSPYEWYNPHPCNPDSDVVENNFTMLNSFWFGVGALMQQG 123          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Sea Lamprey
Match: gria1b (glutamate receptor, ionotropic, AMPA 1b [Source:ZFIN;Acc:ZDB-GENE-020125-2])

HSP 1 Score: 193.741 bits (491), Expect = 1.000e-52
Identity = 95/180 (52.78%), Postives = 127/180 (70.56%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNR-EVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRD-CSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWW 3985
            G E  P++ S R+V G WWFFTLIIIS+YTANLAAFLTVE+++ PI+S +DLA+QT I YGTL  G++  FF+++ I  Y+KM+ FMK    +VFV +   GV++VR S GKYA+LLES  N++  QR  C  M++G  LDSKGYGI  P GS LRD +  ++LKL +   +  L +KWW
Sbjct:  249 GCEISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESADDLAKQTEIAYGTLDSGSTKEFFQRSKIGVYEKMWLFMKDAEPKVFVRTNADGVSRVRKSKGKYAYLLESTTNEYTEQRKPCDTMKVGGNLDSKGYGIATPKGSALRDAVNLAVLKLNEQGLLDKLKNKWW 428          

HSP 2 Score: 117.857 bits (294), Expect = 1.669e-27
Identity = 57/145 (39.31%), Postives = 90/145 (62.07%), Query Frame = 3
Query: 1932 QAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELD-------------------------------NQFNLLNSLWFTVGALVRQG 2273
            +A++A+AP+TIS  RE+V+DF+ PFMSLG+SI+  KP+K  PG+ SFL P+++ +W+ II AYIGVS ++F +S  +PYEW +    DD+++++                               N+F + NSLWF++GA ++QG
Sbjct:  109 RADIAIAPLTISLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVISFLDPLAYEIWMCIIFAYIGVSVVLFLVSRFSPYEWQR----DDDTDVEPNGNGGAGGGGGGGGSGGGAGGGSGGGGEPPNEFGIFNSLWFSLGAFMQQG 249          

HSP 3 Score: 50.0618 bits (118), Expect = 3.865e-6
Identity = 29/89 (32.58%), Postives = 48/89 (53.93%), Query Frame = 1
Query: 1336 RPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            R + VTTI+  P+   K+   + + ++ N + + G+C+DL +EI       +   +V D  YG       NPD ++ WNGM+GEL+  R
Sbjct:   31 RTVIVTTILDAPYFMLKK---NHEQFEGN-DKYEGYCVDLGQEIAIHEQFEYKWSVVPDGAYG-----ARNPD-TKVWNGMVGELVYGR 109          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002503.1 (pep scaffold:Pmarinus_7.0:GL492163:1:1446:1 gene:ENSPMAG00000002286.1 transcript:ENSPMAT00000002503.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 159.844 bits (403), Expect = 5.874e-45
Identity = 76/151 (50.33%), Postives = 111/151 (73.51%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSG 3901
            G E  P+A STR+V G WWFFTLI+IS+YTANLAAFLTVE++  PI S +DLA+QT I+YG ++ G++ TFF+K+ I TY+KM+ FM  +++   V + E+G+++   ++  +A L+ES   +  +QR C+L +IG L+DSKGYG+G P G
Sbjct:   40 GSELMPRALSTRIVGGIWWFFTLILISSYTANLAAFLTVERMESPIGSADDLAKQTKIEYGAVRDGSTMTFFKKSRISTYEKMWAFMSSRHQTALVKNNEEGIHRALTTD--FAMLMESTTIEHVTQRYCNLTQIGGLIDSKGYGVGMPLG 188          

HSP 2 Score: 55.4546 bits (132), Expect = 1.085e-8
Identity = 23/39 (58.97%), Postives = 31/39 (79.49%), Query Frame = 3
Query: 2160 TPYEWLQIHPCDDNSE-LDNQFNLLNSLWFTVGALVRQG 2273
            +PYEW   HPC+ +S+ L+N F +LNSLWF VGAL++QG
Sbjct:    2 SPYEWKNPHPCNPDSDVLENNFTMLNSLWFGVGALMQQG 40          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Sea Lamprey
Match: GRIA4 (glutamate ionotropic receptor AMPA type subunit 4 [Source:HGNC Symbol;Acc:HGNC:4574])

HSP 1 Score: 98.2117 bits (243), Expect = 8.485e-21
Identity = 120/494 (24.29%), Postives = 195/494 (39.47%), Query Frame = 1
Query:  214 QNANKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSC-RFPESLDRYPMDIQLTPNCD----------QYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDL-------KKNKKLLRELGKSKKYRYFLVDIPYWQITDFLI-MASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVR---PMKCKSNDTWRLGSSLINAMTAAENLY--GITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRI--------EPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYD--TNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELI 1593
             N+N   +   +   V T+T+D  NSF   +  C+    G+ AM S +    +  ++S C+  H+  +  S  + P S    P  I+L P             ++ K +Y Y T   +W                  LL         R W    L       +  ++LLR+L   +  R  ++D   +++T  L+ + +V N     F +I  +     VD+  F+     N++ F + D     RM      V   L+   +             F    A+ +D V  +++    L K R  + R      C +N     G   ++   A +N++  G+T  I FNE  QR NY L +MEL   G  K G        V P  I        E  +    +  + + VTTI+  P+  Y      R+NY+  T  + + G+C DL  EI   +G N+   LV D  YG      +    ++ WNGM+GEL+
Sbjct:   25 HNSNLNESDVPFGLTVYTDTIDLPNSFTFTKAFCSQFSRGVFAMFSVLDRRSASSLTSFCAALHVSMVTPSAPQEPPS----PFLIELQPPLGWPLLHLIDRYEWGKFVYLYDTDRGLW------------------LLQSVVQEATRRGWAMMALSIEGLSEEALRQLLRDLA-VRGERNIVLDCETYRVTSTLLQVVNVGNHVK-GFHYIIANLGFLDVDLDVFRYGG-ANVSGFQMVD-----RMAP---QVIAFLDKWHKQSPHVYPGSDSLHFKYTSALAYDGVFVISQAFRALYKQRFDLHREGNAGDCLANPAVPWGKG-VDIQRALKNVHINGLTNNIQFNELGQRSNYTLGVMELRPTGPTKLG----SIMCVHPGGILVRHQALGEDGINSSLHQNKTVIVTTILDAPYFMY------RKNYEQLTGNDRFEGYCADLGEEIARHVGFNYRWKLVPDGAYG------SRDTETKIWNGMVGELV 468          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007790.1 (pep scaffold:Pmarinus_7.0:GL476616:796957:798868:-1 gene:ENSPMAG00000007047.1 transcript:ENSPMAT00000007790.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 85.5001 bits (210), Expect = 1.350e-17
Identity = 52/176 (29.55%), Postives = 95/176 (53.98%), Query Frame = 2
Query: 3467 PQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRD-CSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNE 3991
            P + + +++   WW F L++ STYTANLAAFLT E     I+S++DL +Q + +YGT  G     F   +    Y  +++ ++ NR   V S +KG+ +   +   Y F+ E++   F +++D C L+            + FP GS L   +++++ +L+ S ++A L  +W+ +
Sbjct:  105 PLSMAGKIIVYTWWCFVLVVCSTYTANLAAFLTAEGQT-SIRSLKDLTRQMDARYGTYSGYRIVGFLNSSARDPYMTVWRHIRDNR-TMVASPKKGLEQAEKAG--YIFIGEASAV-FTAKKDHCDLIISDRRFFRHRLALPFPPGSRLAAEVSRAVTELEDSGQLAELERQWFAQ 275          

HSP 2 Score: 59.3066 bits (142), Expect = 3.674e-9
Identity = 31/100 (31.00%), Postives = 54/100 (54.00%), Query Frame = 3
Query: 1977 EKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQ-FNLLNSLWFTVGALVRQG 2273
            ++ +  + P +  G  I+    +   P +F FL P++  +W+ ++SA  G+S ++F +S + P EW  +      S    Q FNL+NS WF   A+V+QG
Sbjct:    1 DERLALSPPILENGYHIIMKGKEVNEPRIFQFLEPLNTLLWISMLSACAGISVVLFVLSLLHPDEWYHLAKRKLVSREQGQSFNLVNSFWFVFSAMVKQG 100          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Nematostella
Match: EDO41293 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4J7])

HSP 1 Score: 138.658 bits (348), Expect = 5.446e-36
Identity = 73/188 (38.83%), Postives = 118/188 (62.77%), Query Frame = 2
Query: 3467 PQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRD-CSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKES 4027
            P++ S R+++ ++W FTLI+ISTYTANLAAF T +     I S+EDL  Q   KYG  +GG+ F+FFEK+ +  Y+KMF  M      FV  T++GVN+ R +   +A+L +    D+Y+QR  C+ M + +LLD+K Y +G    S   D ++ +IL+L++  ++  + +KWW+ M+    C+ K++
Sbjct:   75 PRSGSGRVLAASFWLFTLILISTYTANLAAFFTAQNSKSTINSLEDLVAQDVFKYGVQQGGSLFSFFEKSKVRIYKKMFGKM-STLNTFVKGTDEGVNRSRTT--PFAYLTDQPSLDYYNQRKPCNTMLVKNLLDAKSYALGLQRNSEWTDGISVAILQLREEGEVERIRYKWWDAMS---ECNLKDA 256          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Nematostella
Match: EDO41291 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4J5])

HSP 1 Score: 128.257 bits (321), Expect = 2.161e-31
Identity = 73/189 (38.62%), Postives = 119/189 (62.96%), Query Frame = 2
Query: 3440 IASI---GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILP-IKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRD-CSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNE 3991
            +ASI   G +  P+++S R+++ A+W FTLI+ISTYTANLAA+ T E+ I   I S+EDL  Q+ +KYG  +GG+ FTFF ++ +  Y  MF+ M K++E FV+ T+ GV K R     +A+L +    D+Y+ R  C+ M + +LLD+K Y +     S   + ++ +IL+L++  +I     +WW++
Sbjct:  180 VASILQQGGDNTPRSTSGRVLAAAFWLFTLILISTYTANLAAYFTGERGIRSKINSLEDLVSQSTVKYGVQEGGSLFTFFSESKVDIYATMFQQM-KSQETFVNGTQYGVLKARTQ--PFAYLTDQPSLDYYNMRKPCNTMLVKNLLDAKSYALAMRRNSEWTNLISVAILELREEGEIEKSRLRWWDD 365          

HSP 2 Score: 80.1073 bits (196), Expect = 1.552e-15
Identity = 38/118 (32.20%), Postives = 69/118 (58.47%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTVGALVRQG 2273
             +  +A++A+ P+TI+ ERE+V+DF+ PF+   ++++  +P  E   LF+FL P    +W+  +     VS I++F+   +P  +          E D +F+L NSLWF V ++++QG
Sbjct:   71 LVREEADIALGPITITAEREEVIDFSKPFLDFRIAMILQQPTGEEVNLFAFLLPFDEKLWLTTLGVVGLVSLIVWFLDRFSPQGYKTQAEKSGEGEGD-EFSLSNSLWFAVASILQQG 187          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Nematostella
Match: EDO30203 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1G4])

HSP 1 Score: 127.487 bits (319), Expect = 1.486e-29
Identity = 68/175 (38.86%), Postives = 109/175 (62.29%), Query Frame = 2
Query: 3467 PQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDF--YSQRDC-SLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKW 3982
            P +SS R+    +WF  LI++STYTANLAAFLT+++   PI SV+DLA+Q +I YGT+       FFE A +P++  M+++M+ +   FV+++ +G+ KV N N  YAF+ +SA+ +F  ++Q  C +L+  G +    GYG G    SP    ++ +IL+L+ +  +  L  KW
Sbjct:  649 PVSSSGRITVAVYWFAMLIVMSTYTANLAAFLTIKRFTSPISSVDDLARQKDISYGTVLNSQPQAFFESASVPSFVTMWQYMRYH-HTFVNNSAEGIEKVMNEN--YAFIWDSAVLEFVAHNQISCGTLITSGSVFGRIGYGFGLAKDSPYTKQLSNAILQLRHAGYMEFLDRKW 820          

HSP 2 Score: 68.1662 bits (165), Expect = 2.152e-11
Identity = 41/123 (33.33%), Postives = 65/123 (52.85%), Query Frame = 3
Query: 1923 INTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQ--IHPCDDNSE-----LDNQFNLLNSLWFTVGALVRQ 2270
            +N +A+LA+  +TIS ER+KV+DFT P+M LGL++L      E    F+ L P  + +W+ I    I V F ++  S  +P+ +    +  C    E     L +  +L+ +LW TV   V Q
Sbjct:  520 LNGRADLAVTSLTISPERQKVIDFTQPYMDLGLTVLIKPDPTEEKNPFAILRPFRYDLWMAIGGTMIIVGFFLWLFSTFSPFGFYGRCVQKCHTKIEPRYLKLHDTLSLVRALWSTVVYYVGQ 642          

HSP 3 Score: 63.1586 bits (152), Expect = 8.633e-10
Identity = 51/190 (26.84%), Postives = 92/190 (48.42%), Query Frame = 1
Query: 1117 GSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIE---PSVVEIGNIVRP-----------LKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR--FIVNQISIN*KR 1638
            G++L++ ++   N  G+  ++ F+ N    +    ++ L   GFQK G+WN +  + L  + E   PS    G +  P           LKV TI   PF+F +     +      +E   G+CI+LLR++   L   F ++LV DN +G       +P  +++WNG++ E++  R    V  ++I+ +R
Sbjct:  362 GATLLDYISQV-NTTGVMNQLGFDSNRSPVDVAFDVVNLRAFGFQKVGYWNVEEGLHLDNKKEIVWPS----GRVYVPTDSTHILENRTLKVVTIAEAPFIFAQTQTNGQGETRVIIE---GYCIELLRKLSEMLRFKFEVYLVPDNNFGA-----QDPV-TKEWNGVVREVLNGRADLAVTSLTISPER 537          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Nematostella
Match: EDO46633 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RPU4])

HSP 1 Score: 108.997 bits (271), Expect = 8.307e-24
Identity = 61/192 (31.77%), Postives = 102/192 (53.12%), Query Frame = 2
Query: 3467 PQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRD-CSLMRIGDLLDSKG-----YGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKE 4024
            P ++S R     WWF   I+ +TYTAN+AAFLT  ++  PI+ +EDL+ QT I YG ++   +  FF+ + +  YQ M+++MK  R +  D T   +   R   GKYAF+ ++ + ++ +++  C  +++    D++      YG   P  SP  + ++  ILKL +S     +I +W  E     P D  E
Sbjct:  581 PVSASGRAAVAVWWFVITILGATYTANMAAFLTTTRMQTPIRRIEDLSSQTEIAYGCVENSLTQNFFQSSSVQRYQMMWEYMKLRRSLQPD-TYTAIE--RTLKGKYAFIHDAPILEYLARKTYCGRIKLIGGYDTRTFGNAHYGFALPKKSPYTNLLSVEILKLVQSGIAKTMIEEWMIERTPCAPLDEAE 769          

HSP 2 Score: 63.929 bits (154), Expect = 5.316e-10
Identity = 39/125 (31.20%), Postives = 65/125 (52.00%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWL-----QIHPCDDNSELDNQFNL--LNSLWFTVGALVRQG 2273
             I  +A+LA+ P+TI+  R KV+DFT PFM+ G+ ++        P  F FL P    +W+ I  A +G+  + +  S V+P+ +       I+     S L  +++L  LNS+W +    + QG
Sbjct:  452 LIQERADLAVGPITITSHRWKVIDFTQPFMTSGIGVVMGTENSPKP-YFRFLEPFKSDLWITIFGAVLGMGVVNWLFSVVSPFGFYGRCVQSINKRVKKSYLKQKYSLSFLNSIWSSAAYYLGQG 575          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Nematostella
Match: EDO35545 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SL56])

HSP 1 Score: 88.9669 bits (219), Expect = 8.926e-18
Identity = 53/178 (29.78%), Postives = 94/178 (52.81%), Query Frame = 2
Query: 3458 EFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIK---YGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKW 3982
            E  P++ S R++   W  F +II+++YTANLAAFL +++    +  ++D   +   K   Y T+   +   +F + +      M+ FM+      V + ++G+ KV+N   K AF+ +S +  + + +DC+L   G+L    GYGIG P GSP  + ++ +IL   +S  +  L   W
Sbjct:  605 EGTPRSFSARVLGMVWAGFAMIIVASYTANLAAFLVLDRPKAVVSGIDDPNLRNPSKQFIYATVANSSVDAYFRRQV--ELSSMYTFMEGYN---VKTAKEGIEKVKNGELK-AFIWDSPVLYYEASKDCTLTTAGELFGRSGYGIGMPKGSPWSNAISLAILNFHESGVMEELETTW 776          

HSP 2 Score: 78.9518 bits (193), Expect = 1.063e-14
Identity = 57/218 (26.15%), Postives = 98/218 (44.95%), Query Frame = 1
Query: 1063 RPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTEN--------GFQKGGFWNDDRFVVLPKRIE--PSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQ--NYDTNM-----------EAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR--FIVNQISIN*KRC 1641
            + L      C+    W  G  L NA+       G TG I FN +  RKN    +M   ++        G  + G  + +  ++ P  +   P+ V + N    L+V T++ +PFV+ K      +  + DTN            +  +GFC+DLL  +G  +   +++ L +D  YG     + N   +++WNGM+GE+I  +   IV  ++IN +R 
Sbjct:  296 KTLTTPTASCRETQQWSSGQELFNALVNVSLPRGTTGPIAFNSDGDRKNAVYRLMNRRQSSSDAVDVVGLYENGHVSINESIIWPGNLNTTPTGVFLSN---HLRVVTLVGEPFVYVKPIPTSGRCSDLDTNTAKHVLCTGTVSDTDSGFCMDLLIRLGEKVNFTYSVSLSEDGSYGSL--RRVNGSDTKQWNGMVGEVIQGKADLIVAALTINNERA 508          

HSP 3 Score: 55.0694 bits (131), Expect = 2.644e-7
Identity = 32/117 (27.35%), Postives = 66/117 (56.41%), Query Frame = 3
Query: 1923 INTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTVGALVRQG 2273
            I  +A+L +A +TI+ ER + ++F+ PF   GL+IL +K  +++  L SFL P    +W+ ++ +   V+ I++ +   +P+   ++   +     +   NL +++WF+ G L+  G
Sbjct:  490 IQGKADLIVAALTINNERAEWIEFSKPFKYQGLTIL-VKKNEQSNSLDSFLRPFQIHLWLLVLLSVHIVAVILYLLDRFSPFGRFKLARKEKE---ETALNLSSAMWFSWGVLLNSG 602          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Medaka
Match: grik4 (glutamate ionotropic receptor kainate type subunit 4 [Source:NCBI gene;Acc:101170037])

HSP 1 Score: 212.616 bits (540), Expect = 9.888e-56
Identity = 104/194 (53.61%), Postives = 139/194 (71.65%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMK-KNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G    P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++ +PI+SV+DLA QT+I+YGT+ GG++ TFF+ +   TYQ+M+ FM  K   VFV STE+G+ +V NSN  YA+LLES MN++Y QR+C+L +IG LLD+KGYG+G P GS  RD    +ILKLQ+  ++  L  KWW+     +  D +  G
Sbjct:  608 GSTIAPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEVPIESVDDLADQTSIEYGTMHGGSTMTFFQNSRYQTYQRMWNFMHSKQPSVFVKSTEEGIARVLNSN--YAYLLESTMNEYYHQRNCNLTQIGGLLDTKGYGVGMPLGSVYRDEFDLAILKLQEENRLEILKRKWWDGGKCPKEEDHRAKG 799          

HSP 2 Score: 145.206 bits (365), Expect = 1.574e-34
Identity = 146/534 (27.34%), Postives = 243/534 (45.51%), Query Frame = 1
Query:   73 MLIFGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHI-SSLCSKFHIPHL------VTSCRFPE--SLDRYPM--DIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLV---DIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKP---AILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIV--RPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            ML    ++ FL++   ST         I  ILD   + SR     I+LA +N NK   R      +V    L  ++ +      C ++  G+VA++ P A   S  I S++C +  +P++      +   +FP   +LD  P   DI L       A ++  + +         LI +   C+L  E LL QF  S+  +   +  D +    LL+E+ +  K    +VD         L  AS   M    +++IFT  + +L+   D+A    + +VNI  FS+          KT+   +  ++S+       + CD    F   P   A+LFD V  +   +  L +S+ +    + CKS+  W  G+SL+N +   E L G+TG I FN   QR NY L IM+ ++ G ++ G W+ +  + + +   P  + + + +    L VTTI+  P+V  K   P+ Q ++ N + + GFC+D+L E+   L   + I LV D  YG        P  +  W GM+GELI+++
Sbjct:    1 MLTAWALLLFLAMLVRSTSG-GPLSFRIAAILDDPMECSRGERLAITLAKENINKSSNRSTTGKLEVDIFELLRDSEYEMGETMCQIMSKGVVAVLGPSASPASNSIISNICGEKEVPYVKVAPEDILKVQFPRFTTLDLRPTNTDISLA-----VAGLLTFFNSTTAC-----LICAQAECLLNLEQLLRQFLISKETLSVRMLDDSQDPTPLLKEI-RDDKTATIIVDANATMSHIILERASELGMLSVYYTYIFTSLEFTLLRLDDVA----DQRVNIVGFSV--------FNKTHPFFQDFVQSL--NRSWQENCD-HAPFAGTPLSSALLFDAVYAVVAAVQELNRSQNVGATQLSCKSSKIWEHGTSLMNYLRMVE-LEGLTGHIEFNSKGQRSNYALRIMQNSKEGLRQIGLWHSEDGLSMERM--PLSINVTDTLFNTTLTVTTILESPYVMLK---PNYQEFEGN-DRYEGFCVDMLEELAELLKFKYRIRLVGDGLYGV-------PGANGTWTGMVGELISRK 493          

HSP 3 Score: 119.783 bits (299), Expect = 9.308e-27
Identity = 56/120 (46.67%), Postives = 83/120 (69.17%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             I+ +A+LA+A +TI+ EREKV+DF+ PFM+LG+SI++       PG FSFL P S  VW++++ AY+ VS ++F ++ +TPYEW   HPC     + L NQ++L NS WF VG  ++QG
Sbjct:  489 LISRKADLAVAGLTITAEREKVIDFSKPFMTLGISIMYRVHLGRRPGYFSFLDPFSPGVWLFMLLAYLAVSCVLFLVARLTPYEWYNPHPCLKGQCNLLINQYSLGNSFWFPVGGFMQQG 608          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Medaka
Match: ENSORLT00000001416.2 (glutamate receptor ionotropic, kainate 5 [Source:NCBI gene;Acc:101155942])

HSP 1 Score: 207.994 bits (528), Expect = 2.167e-54
Identity = 102/194 (52.58%), Postives = 136/194 (70.10%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++  PI+S +DLA QTNI+YGT+ GG++ TFF  +   TYQ+M+ +M  K   VFV STE+G+ +V NS  KYAFL+ES MN++Y   +C+L +IG LLD+KGYGIG P GSP R  +T  IL++Q+S ++  L  +WW      +  D +  G
Sbjct:  586 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEAPIESADDLADQTNIQYGTIHGGSTMTFFMNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNS--KYAFLMESTMNEYYRSLNCNLTQIGGLLDTKGYGIGMPLGSPYRHEITLGILQMQESNRLEILKRRWWEGGQCPKEEDHRAKG 777          

HSP 2 Score: 140.198 bits (352), Expect = 4.461e-33
Identity = 129/496 (26.01%), Postives = 215/496 (43.35%), Query Frame = 1
Query:  145 LLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRH-ISSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            L     ILD   D  R     ++LA +N N  +   + A  +V    L  ++ +      C ++  G+V++I P +   S   IS +C +  IPH+      PE   + P      + L P+ +  +  I   +     +    LI +   C+L+ E L+  F  SR  +   +  D      LL+E+ +  K    ++D         L  A+   M    + +I T  D  L+ +    +N++ NI  FS+        + +T+    + + S+    +               A++FD V+ +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E   G+TG I FN   QR NY L I+E    G+++ G W  +  +V+               + L VTTI+  P+V  K    D Q  D     + GFC+D+LRE+   L  +F I LV D  YG        P+GS  W GM+GELI ++
Sbjct:    2 LFPSAAILDDQSDCGRGERLALALARENINNNIEGASQARVEVDVYELQKDSQYETTDTMCQILPKGVVSVIGPASSPASGSTISHICGEKEIPHVKIG---PEETPKLPYLRFASVTLYPSNEDMSLAIGSILRSFG-YPTASLICAKAECLLRLEELVRHFLISRETLSVRMLDDSLDPTPLLKEI-RDDKVATIIIDANASISYQILRKANELGMMTAFYKYILTTMDFPLLKMDDV-VNDQSNILGFSM--------LNRTHPFFSEFIRSLNLSWREGCHISPYPGPALSSALMFDGVHVVVGAVRELNRSQEIGVKPLSCTSPLIWQHGTSLMNYLRMVE-YDGLTGHIEFNSKGQRTNYTLKILEKHPAGYKEIGTWYSNNTLVMNSTSLDLNASQTLANKSLIVTTILENPYVMRKSNYQDYQGNDQ----YEGFCVDMLRELAGILKFSFKIKLVDDGLYGAP-----EPNGS--WTGMVGELIDRK 471          

HSP 3 Score: 119.398 bits (298), Expect = 1.151e-26
Identity = 56/120 (46.67%), Postives = 82/120 (68.33%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             I+ +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   +PC  +    L+NQ+ L NSLWF VG  ++QG
Sbjct:  467 LIDRKADLAVAGFTITSEREKVIDFSKPFMTLGISILYRVHLGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPYPCFRERRDILENQYTLGNSLWFPVGGFMQQG 586          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Medaka
Match: grik5 (glutamate ionotropic receptor kainate type subunit 5 [Source:NCBI gene;Acc:101174774])

HSP 1 Score: 208.379 bits (529), Expect = 2.579e-54
Identity = 102/194 (52.58%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++  PI+S +DLA QTNI+YGT+ GG++ TFF  +   TYQ+M+ +M  K   VFV STE+G+ +V NS  KYAFL+ES MN+++   +C+L +IG LLD+KGYGIG P GSP RD +T +IL+LQ++ ++  L  +WW      +  D +  G
Sbjct:  606 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEAPIESPDDLADQTNIEYGTIHGGSTMTFFMNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNS--KYAFLMESTMNEYHRGLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQENNRLEILKRRWWEGSQCLKEEDHRAKG 797          

HSP 2 Score: 135.191 bits (339), Expect = 1.987e-31
Identity = 128/487 (26.28%), Postives = 225/487 (46.20%), Query Frame = 1
Query:  190 RKVISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRH-ISSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQF---SFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKK-CDIEKAFMS-KPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFW-NDDRFVVLPKRIEPSVVE-IGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            R  ++LA +N N  +     A  +V    L  ++ +      C ++  G+V++I P +   S   +S +C +  IPH+      PE   R P      + L P+ +  +  I   +     +    L+ +   C+L+ E L+ +F  SR  +   +  D      LL+E+ +  K    ++D        +LI+  V + T   F   +  + D+   ++D     ++ + NI  FS+  T H                LE I  +  + ++ CD+     +   A++FD V+ +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E   G+TG++ FN   QR NY L I+E    G ++ G W +++   +    ++ +V E + N  + L VTTI+  P+V  K+   D Q  D     + GFC+D+LRE+ + L  +F I LV D  YG        P+GS  W GM+GELI ++
Sbjct:   42 RLALALARENINSVMEGPARARVEVDIYELQKDSQYDTTDTMCQILPKGVVSVIGPASSPASGSTVSHICGEKEIPHVKIG---PEETPRLPYLRFASVTLYPSNEDLSLAIGAILRSFS-YPSASLVCAKAECLLRLEELVRRFLISRETLSVRMLDDNLDPTPLLKEI-RDDKVATIIIDAN--ASVSYLILKKVRSETSICFFRTTHTYLDFKLLVLDDI---VDEQSNIVGFSMFNTTHPFY-------------LEFIRSLNLSWREGCDLTYPGPALSSALMFDAVHVVVGAVRELNRSQEIGVKPLSCTSPQIWQHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLRILEKHRGGHKEIGIWYSNNTLAMNSTSLDINVSETLAN--KTLIVTTILENPYVMRKDNYQDFQGNDQ----YEGFCVDMLRELADILKFSFKIKLVDDGLYGAP-----EPNGS--WTGMVGELINRK 491          

HSP 3 Score: 125.176 bits (313), Expect = 2.346e-28
Identity = 58/120 (48.33%), Postives = 82/120 (68.33%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC  +    L+NQ+ L NSLWF VG  ++QG
Sbjct:  487 LINRKADLAVAAFTITSEREKVIDFSKPFMTLGISILYRVQLGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCMRERRDMLENQYTLGNSLWFPVGGFMQQG 606          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Medaka
Match: grik5 (glutamate ionotropic receptor kainate type subunit 5 [Source:NCBI gene;Acc:101174774])

HSP 1 Score: 208.379 bits (529), Expect = 3.595e-54
Identity = 102/194 (52.58%), Postives = 138/194 (71.13%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++  PI+S +DLA QTNI+YGT+ GG++ TFF  +   TYQ+M+ +M  K   VFV STE+G+ +V NS  KYAFL+ES MN+++   +C+L +IG LLD+KGYGIG P GSP RD +T +IL+LQ++ ++  L  +WW      +  D +  G
Sbjct:  616 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEAPIESPDDLADQTNIEYGTIHGGSTMTFFMNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNS--KYAFLMESTMNEYHRGLNCNLTQIGGLLDTKGYGIGMPLGSPFRDEITLAILQLQENNRLEILKRRWWEGSQCLKEEDHRAKG 807          

HSP 2 Score: 141.354 bits (355), Expect = 2.400e-33
Identity = 130/484 (26.86%), Postives = 223/484 (46.07%), Query Frame = 1
Query:  190 RKVISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRH-ISSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSI--TDHSIMERMTKTYENVEKLLESIVEMEKTSKK-CDIEKAFMS-KPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFW-NDDRFVVLPKRIEPSVVE-IGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            R  ++LA +N N  +     A  +V    L  ++ +      C ++  G+V++I P +   S   +S +C +  IPH+      PE   R P      + L P+ +  +  I   +     +    L+ +   C+L+ E L+ +F  SR  +   +  D      LL+E+ +  K    ++D         L  AS   MT   + +I T  D  L+ +    ++ + NI  FS+  T H                LE I  +  + ++ CD+     +   A++FD V+ +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E   G+TG++ FN   QR NY L I+E    G ++ G W +++   +    ++ +V E + N  + L VTTI+  P+V  K+   D Q  D     + GFC+D+LRE+ + L  +F I LV D  YG        P+GS  W GM+GELI ++
Sbjct:   51 RLALALARENINSVMEGPARARVEVDIYELQKDSQYDTTDTMCQILPKGVVSVIGPASSPASGSTVSHICGEKEIPHVKIG---PEETPRLPYLRFASVTLYPSNEDLSLAIGAILRSFS-YPSASLVCAKAECLLRLEELVRRFLISRETLSVRMLDDNLDPTPLLKEI-RDDKVATIIIDANASVSYLILKKASELGMTSAFYKYILTTMDFPLLRLDDI-VDEQSNIVGFSMFNTTHPFY-------------LEFIRSLNLSWREGCDLTYPGPALSSALMFDAVHVVVGAVRELNRSQEIGVKPLSCTSPQIWQHGTSLMNYLRMVE-YDGLTGRVEFNSKGQRTNYTLRILEKHRGGHKEIGIWYSNNTLAMNSTSLDINVSETLAN--KTLIVTTILENPYVMRKDNYQDFQGNDQ----YEGFCVDMLRELADILKFSFKIKLVDDGLYGAP-----EPNGS--WTGMVGELINRK 501          

HSP 3 Score: 124.79 bits (312), Expect = 3.461e-28
Identity = 58/120 (48.33%), Postives = 82/120 (68.33%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             IN +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   HPC  +    L+NQ+ L NSLWF VG  ++QG
Sbjct:  497 LINRKADLAVAAFTITSEREKVIDFSKPFMTLGISILYRVQLGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPHPCMRERRDMLENQYTLGNSLWFPVGGFMQQG 616          
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Medaka
Match: ENSORLT00000026787.1 (glutamate receptor ionotropic, kainate 5 [Source:NCBI gene;Acc:101155942])

HSP 1 Score: 208.379 bits (529), Expect = 4.619e-54
Identity = 102/194 (52.58%), Postives = 136/194 (70.10%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFM-KKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESG 4030
            G E  P+A STR VSG WW FTLIIIS+YTANLAAFLTV+++  PI+S +DLA QTNI+YGT+ GG++ TFF  +   TYQ+M+ +M  K   VFV STE+G+ +V NS  KYAFL+ES MN++Y   +C+L +IG LLD+KGYGIG P GSP R  +T  IL++Q+S ++  L  +WW      +  D +  G
Sbjct:  617 GSEIMPRALSTRCVSGVWWAFTLIIISSYTANLAAFLTVQRMEAPIESADDLADQTNIQYGTIHGGSTMTFFMNSRYQTYQRMWNYMYSKQPSVFVKSTEEGIARVLNS--KYAFLMESTMNEYYRSLNCNLTQIGGLLDTKGYGIGMPLGSPYRHEITLGILQMQESNRLEILKRRWWEGGQCPKEEDHRAKG 808          

HSP 2 Score: 150.214 bits (378), Expect = 5.584e-36
Identity = 133/515 (25.83%), Postives = 229/515 (44.47%), Query Frame = 1
Query:   91 VIFFLSICPISTKELSK-KLLNIGVILDASDDISRK---VISLAVQNANKELARFNYA-FQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRH-ISSLCSKFHIPHLVTSCRFPESLDRYP----MDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFE-SRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            V+F L++ P+S  +++   ++ +  ILD   D  R     ++LA +N N  +   + A  +V    L  ++ +      C ++  G+V++I P +   S   IS +C +  IPH+      PE   + P      + L P+ +  +  I   +     +    LI +   C+L+ E L+  F  SR  +   +  D      LL+E+ +  K    ++D         L  A+   M    + +I T  D  L+ +    +N++ NI  FS+        + +T+    + + S+    +               A++FD V+ +   +  L +S+ + V+P+ C S   W+ G+SL+N +   E   G+TG I FN   QR NY L I+E    G+++ G W  +  +V+               + L VTTI+  P+V  K    D Q  D     + GFC+D+LRE+   L  +F I LV D  YG        P+GS  W GM+GELI ++
Sbjct:   14 VVFLLTMAPLSFSQVNTLSVVRMAAILDDQSDCGRGERLALALARENINNNIEGASQARVEVDVYELQKDSQYETTDTMCQILPKGVVSVIGPASSPASGSTISHICGEKEIPHVKIG---PEETPKLPYLRFASVTLYPSNEDMSLAIGSILRSFG-YPTASLICAKAECLLRLEELVRHFLISRETLSVRMLDDSLDPTPLLKEI-RDDKVATIIIDANASISYQILRKANELGMMTAFYKYILTTMDFPLLKMDDV-VNDQSNILGFSM--------LNRTHPFFSEFIRSLNLSWREGCHISPYPGPALSSALMFDGVHVVVGAVRELNRSQEIGVKPLSCTSPLIWQHGTSLMNYLRMVE-YDGLTGHIEFNSKGQRTNYTLKILEKHPAGYKEIGTWYSNNTLVMNSTSLDLNASQTLANKSLIVTTILENPYVMRKSNYQDYQGNDQ----YEGFCVDMLRELAGILKFSFKIKLVDDGLYGAP-----EPNGS--WTGMVGELIDRK 502          

HSP 3 Score: 119.398 bits (298), Expect = 1.384e-26
Identity = 56/120 (46.67%), Postives = 82/120 (68.33%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPC--DDNSELDNQFNLLNSLWFTVGALVRQG 2273
             I+ +A+LA+A  TI+ EREKV+DF+ PFM+LG+SIL+       PG FSFL P S  VW++++ AY+ VS ++F  + ++PYEW   +PC  +    L+NQ+ L NSLWF VG  ++QG
Sbjct:  498 LIDRKADLAVAGFTITSEREKVIDFSKPFMTLGISILYRVHLGRKPGYFSFLDPFSPAVWLFMLLAYLAVSCVLFLAARLSPYEWYNPYPCFRERRDILENQYTLGNSLWFPVGGFMQQG 617          
BLAST of Glutamate receptor, ionotropic kainate vs. Planmine SMEST
Match: SMESG000005199.1 (SMESG000005199.1)

HSP 1 Score: 1059.67 bits (2739), Expect = 0.000e+0
Identity = 509/510 (99.80%), Postives = 510/510 (100.00%), Query Frame = 1
Query:   73 MLIFGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRKVISLAVQNANKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            MLIFGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRKVISLAVQNANKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITK+
Sbjct:    1 MLIFGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRKVISLAVQNANKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKQ 510          

HSP 2 Score: 698.353 bits (1801), Expect = 0.000e+0
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHPDLTNSKSRERLNLLDHQSGNVNDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSPCLDRSLGFDSSHDGLSSIPNSKPLWGLNSNL 4513
            GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTELNVYQIGGIFILLVGGFIFAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHPDLTNSKSRERLNLLDHQSGNVNDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSPCLDRSLGFDSSHDGLSSIPNSKPLWGLNSNL
Sbjct:  623 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTELNVYQIGGIFILLVGGFIFAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHPDLTNSKSRERLNLLDHQSGNVNDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSPCLDRSLGFDSSHDGLSSIPNSKPLWGLNSNL 976          

HSP 3 Score: 239.195 bits (609), Expect = 1.785e-64
Identity = 114/118 (96.61%), Postives = 115/118 (97.46%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTVGALVRQG 2273
             I  QAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTVGAL+RQG
Sbjct:  506 LITKQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTVGALMRQG 623          
BLAST of Glutamate receptor, ionotropic kainate vs. Planmine SMEST
Match: SMESG000005199.1 (SMESG000005199.1)

HSP 1 Score: 1055.82 bits (2729), Expect = 0.000e+0
Identity = 509/510 (99.80%), Postives = 510/510 (100.00%), Query Frame = 1
Query:   73 MLIFGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRKVISLAVQNANKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR 1602
            MLIFGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRKVISLAVQNANKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITK+
Sbjct:    1 MLIFGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRKVISLAVQNANKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKQ 510          

HSP 2 Score: 224.942 bits (572), Expect = 4.974e-62
Identity = 108/111 (97.30%), Postives = 108/111 (97.30%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTV 2252
             I  QAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTV
Sbjct:  506 LITKQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTV 616          
BLAST of Glutamate receptor, ionotropic kainate vs. Planmine SMEST
Match: SMESG000005201.1 (SMESG000005201.1)

HSP 1 Score: 778.089 bits (2008), Expect = 0.000e+0
Identity = 363/521 (69.67%), Postives = 437/521 (83.88%), Query Frame = 1
Query:   82 FGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRKVISLAVQNANKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR--FIVNQISIN*KR 1638
            FG  +  L IC   ++E  K+ L IGVIL+ +DDISRKVI +A+Q AN ELA+ NYA QVKTETL++N+SF+ IRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLD+YPMDIQ+TP+CDQYAK+IYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNK+L  EL KS+KYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIF+DWDA+LVDIAAFKINNKVNITTFSI DH ++ +      +++ L++SI++MEK+SK  DIE  FMS PAILFD ++ L KGII +LK+RP  V+P+KC SN  W LG SL+NA+T+AE + G TGKI+FNE +QR+NYNLS+M LTENGF++ G W +D+ +   K+ E    E+  + +PLKVTTII+ PFVFYKEG P+R +YDTN++AWNGFCIDLL+EIG+DLG+NF+IHLVKD  +G  + N+T PDGSEKW+GMIGELI+K    +V  ++I+ +R
Sbjct:    4 FGVFLLLLFICSTESQESPKENLTIGVILNYNDDISRKVIGMAIQKANIELAKLNYALQVKTETLEENSSFFVIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDKYPMDIQITPDCDQYAKIIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKQLFWELSKSQKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFSDWDANLVDIAAFKINNKVNITTFSIVDHKLIAKTKDVQSDLKSLIDSIIKMEKSSKNADIETKFMSTPAILFDAISTLLKGIIHMLKTRPFSVKPLKCSSNTIWNLGPSLVNAITSAEAIQGFTGKIIFNEKSQRENYNLSVMGLTENGFEESGRWVEDQLITSIKKQEAVSTEVDKVKQPLKVTTIINLPFVFYKEGIPNRNDYDTNIDAWNGFCIDLLKEIGSDLGLNFSIHLVKDGLFGTVIPNETYPDGSEKWSGMIGELISKEVDLVVAPMTISYER 524          

HSP 2 Score: 474.167 bits (1219), Expect = 3.664e-146
Identity = 249/348 (71.55%), Postives = 282/348 (81.03%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHPDLTNSKSRERLNLLDHQSGNVNDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSPCLDRSLGFDSSHDGLSSIPNSKPLW 4495
            GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDL  QT IKYGTL+GG S  FFE ALI TY+KM  FMKKN +  V +T+ GV KVRNS GKYAFLLESAMND+Y+QRDC L+R+GDLLDSKGYGIGFP+GSPLRD MT+SILKLQKSQKIANLIHKWWNE+N+TEPCD+K+ GS+P  LNV Q+GG+F LL  GFIFA  VSIIELYVD+  KKSQWK+PF K+LKKELQFAMRFMSSSKKP  P+  N KSRER  +LDH S   N+ S+    N SN  SK + +Q   + RQ  + +DSPC +RS  F SSHD L+SI N+K LW
Sbjct:  623 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLVNQTKIKYGTLRGGASQAFFENALIYTYKKMDAFMKKNPDSMVATTDDGVAKVRNSKGKYAFLLESAMNDYYAQRDCKLIRVGDLLDSKGYGIGFPTGSPLRDKMTESILKLQKSQKIANLIHKWWNEINVTEPCDTKDKGSIPQSLNVNQVGGVFFLLGAGFIFAGFVSIIELYVDFREKKSQWKMPFCKFLKKELQFAMRFMSSSKKPTRPNNNNGKSRERQLMLDHHSILPNEPSVAHLLNRSN--SKLDGNQYVTSGRQTLSGLDSPCNERSDYFGSSHDTLASINNTKYLW 968          

HSP 3 Score: 196.823 bits (499), Expect = 6.749e-51
Identity = 93/118 (78.81%), Postives = 102/118 (86.44%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTVGALVRQG 2273
             I+ + +L +APMTISYEREKVVDFTTPFMSLGLSI+FL P+KEAPGLFSFLSPIS PVWVY+IS YI  S IIFFISH+TPYEW+  HPCDD S   NQFNLLNSLWFTVGALVRQG
Sbjct:  506 LISKEVDLVVAPMTISYEREKVVDFTTPFMSLGLSIMFLAPQKEAPGLFSFLSPISPPVWVYVISVYILTSLIIFFISHITPYEWMISHPCDDESSKHNQFNLLNSLWFTVGALVRQG 623          
BLAST of Glutamate receptor, ionotropic kainate vs. Planmine SMEST
Match: SMESG000005199.1 (SMESG000005199.1)

HSP 1 Score: 672.929 bits (1735), Expect = 0.000e+0
Identity = 342/342 (100.00%), Postives = 342/342 (100.00%), Query Frame = 2
Query: 3488 MVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHPDLTNSKSRERLNLLDHQSGNVNDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSPCLDRSLGFDSSHDGLSSIPNSKPLWGLNSNL 4513
            MVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTELNVYQIGGIFILLVGGFIFAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHPDLTNSKSRERLNLLDHQSGNVNDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSPCLDRSLGFDSSHDGLSSIPNSKPLWGLNSNL
Sbjct:    1 MVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTELNVYQIGGIFILLVGGFIFAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHPDLTNSKSRERLNLLDHQSGNVNDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSPCLDRSLGFDSSHDGLSSIPNSKPLWGLNSNL 342          
BLAST of Glutamate receptor, ionotropic kainate vs. Planmine SMEST
Match: SMESG000005192.1 (SMESG000005192.1)

HSP 1 Score: 640.188 bits (1650), Expect = 0.000e+0
Identity = 294/521 (56.43%), Postives = 398/521 (76.39%), Query Frame = 1
Query:   82 FGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRKVISLAVQNANKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPSRHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYITQVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLRELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVDIAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKCDIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGSSLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWNDDRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTNMEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWNGMIGELITKR--FIVNQISIN*KR 1638
            F F++  L   PI +KELSK  L IGVI +A+DD+SRKV+S+AVQNAN +L R+NY+F++ TET+DDN SF  IRK CNL+++GLVA+I+P++E+PSRH+SS  ++FHIPHLVT C+FPES   YPM+I+L PNCD YA +I+QYITQ+E W +MI+IH SDNCI+K+E+LLNQFE  FKMRQW+H DLKKNK+ LR+L KS+K+++FLVD+PYW+I DFLIM S HN+T P  SFIFTDWDA ++D++ FKINNKVNIT FS+ + S++  +   ++N+  L+E+I E  K SKK  I    ++ PAI+ D++N L +G+IRL+K +P+  +PMKC +N TW  G SL NA+T+ +++               K YNLS+MELTENGF K G+WN + FV+ P + +  V E   +++PL+VTTI+S PFVFYK+  PD + +DT+++AWNGFCIDLL EIG DL +NF+IHLVKDNKYG  V+N+TNPDGSEKWNGMIGELI+K    +V  ++I+ +R
Sbjct:    7 FPFLLMLLG-RPILSKELSKIYLTIGVIFEANDDVSRKVVSVAVQNANSQLMRYNYSFRIVTETVDDNGSFSVIRKACNLLQNGLVALIAPLSEIPSRHLSSFSNRFHIPHLVTKCQFPESSASYPMNIKLYPNCDAYANIIFQYITQIEAWGEMIIIHKSDNCIMKYEYLLNQFEYHFKMRQWIHGDLKKNKEFLRKLAKSRKHKHFLVDLPYWEIRDFLIMVSTHNLTGPAHSFIFTDWDARILDLSVFKINNKVNITAFSLINDSLVTSLRTRHKNLNSLMENIFESRKLSKKSFINTNLLAAPAIIIDSINVLMEGLIRLIKLQPVFTQPMKCTTNTTWNQGISLFNAITSVDHI---------------KQYNLSVMELTENGFMKKGYWNGNNFVIFPPKSKSPVTETNEVIKPLRVTTIVSLPFVFYKDNSPDIKEHDTSIDAWNGFCIDLLNEIGADLRLNFSIHLVKDNKYGTVVDNETNPDGSEKWNGMIGELISKEADLVVAPMTISYER 511          

HSP 2 Score: 447.973 bits (1151), Expect = 8.421e-137
Identity = 226/343 (65.89%), Postives = 275/343 (80.17%), Query Frame = 2
Query: 3452 GLEFCPQASSTRMVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGTLKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKYAFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSILKLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTELNVYQXXXXXXXXXXXXXXAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKPLHPDLTNSKSRERLNLLDHQSGNVNDVSLEASRNMSNFTSKSESHQSGLNRRQIFTDIDSPCLDRSLGFDSSHDGLSSIPN 4480
            GLEFCPQASSTRMVS AWWFFTLIIISTYTANLAAFLTVEQ++LPIKSVEDL  Q  IKYGTLKGG S  FFE+ALI TY+KM K+M+ N +V V STE+GV+KV  SNGKYAFLLESAMND+Y +R+C+L+R+GD+LDSKGYGIGFP+GSPLRD MTKSILKLQKSQK++NLIHKWWNE NITEPCD+KE GS+PT L+V+Q+GG+F+LL  GFIFA++VS+IELYV YC    +W +PF  Y +KELQFA+RFMSSSKKPL+    ++KSRERL +LD++  N N  S   S ++ N +  S ++ +  N  +IFT++D  C D     + SHD L SI N
Sbjct:  610 GLEFCPQASSTRMVSSAWWFFTLIIISTYTANLAAFLTVEQIVLPIKSVEDLVNQKTIKYGTLKGGASEAFFERALIQTYKKMHKYMENNPDVMVRSTEEGVDKVLKSNGKYAFLLESAMNDYYKERECTLIRVGDMLDSKGYGIGFPTGSPLRDKMTKSILKLQKSQKLSNLIHKWWNEFNITEPCDTKEKGSIPTALDVHQVGGVFLLLGVGFIFASMVSVIELYVTYCEGTCKWNVPFHIYFQKELQFAVRFMSSSKKPLNTYNVDNKSRERLLILDYKQENYNAPSNRTSLHLHNPSKTSRNYPTTTNTPRIFTELDESCNDDKNCLNISHDSLESISN 952          

HSP 3 Score: 205.297 bits (521), Expect = 1.369e-53
Identity = 96/118 (81.36%), Postives = 106/118 (89.83%), Query Frame = 3
Query: 1920 FINTQAELAMAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPVWVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWFTVGALVRQG 2273
             I+ +A+L +APMTISYEREKVVDFTTPFMSLGLSILFL P+KEAPGLFSFLSP+S  VWVY+ISAYIGVS II+FISHVTPYEWLQIHPCDDN    NQFN+LNSLWFTV AL+RQG
Sbjct:  493 LISKEADLVVAPMTISYEREKVVDFTTPFMSLGLSILFLTPQKEAPGLFSFLSPLSPLVWVYVISAYIGVSLIIYFISHVTPYEWLQIHPCDDNGSRQNQFNILNSLWFTVAALMRQG 610          
The following BLAST results are available for this feature:
BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GRIK42.105e-5554.12glutamate ionotropic receptor kainate type subunit... [more]
GRIK42.105e-5554.12glutamate ionotropic receptor kainate type subunit... [more]
GRIK42.105e-5554.12glutamate ionotropic receptor kainate type subunit... [more]
GRIK53.710e-5552.06glutamate ionotropic receptor kainate type subunit... [more]
GRIK53.710e-5552.06glutamate ionotropic receptor kainate type subunit... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
glr-33.366e-5247.28GLutamate Receptor family (AMPA) [Source:UniProtK... [more]
glr-41.213e-4945.23GLutamate Receptor family (AMPA) [Source:UniProtK... [more]
glr-41.248e-4945.23GLutamate Receptor family (AMPA) [Source:UniProtK... [more]
glr-14.467e-4545.03Glutamate receptor 1 [Source:UniProtKB/Swiss-Prot... [more]
glr-24.690e-4434.95Glutamate receptor 2 [Source:UniProtKB/Swiss-Prot... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG111552.902e-5346.72gene:FBgn0039927 transcript:FBtr0333701[more]
CG111552.902e-5346.72gene:FBgn0039927 transcript:FBtr0089195[more]
KaiR1D4.828e-5347.60gene:FBgn0038837 transcript:FBtr0083987[more]
Grik2.264e-4936.84gene:FBgn0038840 transcript:FBtr0335015[more]
Ekar4.696e-4850.00gene:FBgn0039916 transcript:FBtr0110906[more]
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BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-264f5.81.560e-6053.61si:ch211-264f5.8 [Source:ZFIN;Acc:ZDB-GENE-081104-... [more]
grik51.907e-5552.06glutamate receptor, ionotropic, kainate 5 [Source:... [more]
grik42.215e-5553.61glutamate receptor, ionotropic, kainate 4 [Source:... [more]
grik42.215e-5553.61glutamate receptor, ionotropic, kainate 4 [Source:... [more]
grik53.403e-5452.06glutamate receptor, ionotropic, kainate 5 [Source:... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
GRIK42.213e-5753.61glutamate receptor, ionotropic, kainate 4 [Source:... [more]
ogfr6.736e-5753.09opioid growth factor receptor [Source:Xenbase;Acc:... [more]
ogfr4.912e-5653.09opioid growth factor receptor [Source:Xenbase;Acc:... [more]
GRIK45.888e-5653.61glutamate receptor, ionotropic, kainate 4 [Source:... [more]
GRIK46.406e-5653.61glutamate receptor, ionotropic, kainate 4 [Source:... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Grik22.483e-5751.26glutamate receptor, ionotropic, kainate 2 (beta 2)... [more]
Grik41.254e-5554.12glutamate receptor, ionotropic, kainate 4 [Source:... [more]
Grik41.254e-5554.12glutamate receptor, ionotropic, kainate 4 [Source:... [more]
Grik52.507e-5552.06glutamate receptor, ionotropic, kainate 5 (gamma 2... [more]
Grik27.476e-5352.04glutamate receptor, ionotropic, kainate 2 (beta 2)... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q91755|GRIK2_XENLA2.407e-5650.52Glutamate receptor ionotropic, kainate 2 (Fragment... [more]
sp|Q01812|GRIK4_RAT8.768e-5554.12Glutamate receptor ionotropic, kainate 4 OS=Rattus... [more]
sp|Q8BMF5|GRIK4_MOUSE8.768e-5554.12Glutamate receptor ionotropic, kainate 4 OS=Mus mu... [more]
sp|Q16099|GRIK4_HUMAN1.011e-5454.12Glutamate receptor ionotropic, kainate 4 OS=Homo s... [more]
sp|Q5IS46|GRIK4_PANTR1.047e-5454.12Glutamate receptor ionotropic, kainate 4 OS=Pan tr... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2LMY38.951e-7433.60Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4E0RD831.681e-7333.07Glutamate receptor ionotropic kainate 3 OS=Fasciol... [more]
A0A074Z9I46.977e-7333.20Uncharacterized protein OS=Opisthorchis viverrini ... [more]
A0A419PJX91.732e-7233.20Glutamate receptor ionotropic OS=Clonorchis sinens... [more]
A0A4Z2DUZ11.340e-7031.31Glutamate receptor ionotropic, kainate 3 OS=Schist... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
si:ch211-264f5.83.869e-5552.58si:ch211-264f5.8 [Source:ZFIN;Acc:ZDB-GENE-081104-... [more]
grik54.301e-5452.06glutamate ionotropic receptor kainate type subunit... [more]
grik55.760e-5452.06glutamate ionotropic receptor kainate type subunit... [more]
GRIK22.521e-5251.53glutamate receptor ionotropic, kainate 2 [Source:N... [more]
GRIK22.664e-5251.53glutamate receptor ionotropic, kainate 2 [Source:N... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000005084.11.436e-5548.72pep scaffold:Pmarinus_7.0:GL476900:54926:87395:-1 ... [more]
gria1b1.000e-5252.78glutamate receptor, ionotropic, AMPA 1b [Source:ZF... [more]
ENSPMAT00000002503.15.874e-4550.33pep scaffold:Pmarinus_7.0:GL492163:1:1446:1 gene:E... [more]
GRIA48.485e-2124.29glutamate ionotropic receptor AMPA type subunit 4 ... [more]
ENSPMAT00000007790.11.350e-1729.55pep scaffold:Pmarinus_7.0:GL476616:796957:798868:-... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO412935.446e-3638.83Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO412912.161e-3138.62Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO302031.486e-2938.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO466338.307e-2431.77Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO355458.926e-1829.78Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
grik49.888e-5653.61glutamate ionotropic receptor kainate type subunit... [more]
ENSORLT00000001416.22.167e-5452.58glutamate receptor ionotropic, kainate 5 [Source:N... [more]
grik52.579e-5452.58glutamate ionotropic receptor kainate type subunit... [more]
grik53.595e-5452.58glutamate ionotropic receptor kainate type subunit... [more]
ENSORLT00000026787.14.619e-5452.58glutamate receptor ionotropic, kainate 5 [Source:N... [more]
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BLAST of Glutamate receptor, ionotropic kainate vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000005199.11.785e-6499.80SMESG000005199.1[more]
SMESG000005199.14.974e-6299.80SMESG000005199.1[more]
SMESG000005201.13.664e-14669.67SMESG000005201.1[more]
SMESG000005199.10.000e+0100.00SMESG000005199.1[more]
SMESG000005192.18.421e-13756.43SMESG000005192.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003453 ID=SMED30003453|Name=Glutamate receptor, ionotropic kainate|organism=Schmidtea mediterranea sexual|type=transcript|length=4661bp
ATTCATTTCAAATTTAAAAAGAAACAGCGAAATCTAGGGGAAATAAATAG
ATTAGAATTTATTATTTTATAAATGCTCATTTTTGGATTTGTCATTTTCT
TCTTGTCGATTTGTCCAATCTCTACAAAGGAATTATCGAAAAAATTATTA
AATATCGGAGTGATTCTCGATGCCTCCGACGACATCTCAAGGAAAGTCAT
TAGCCTCGCAGTCCAGAATGCCAACAAAGAGTTGGCCAGATTTAATTATG
CATTTCAAGTGAAAACAGAAACTCTCGACGACAACAACAGTTTCTATGCA
ATCAGAAAAGGATGTAACCTTATAAGGCATGGTCTGGTGGCCATGATCAG
TCCGATAGCCGAGGTGCCATCAAGGCATATATCCTCATTATGCAGTAAAT
TCCATATTCCCCATTTGGTGACAAGTTGTAGGTTCCCAGAATCATTAGAT
AGGTATCCTATGGATATTCAATTAACCCCAAATTGTGACCAATATGCCAA
GATGATATATCAGTATATAACTCAAGTTGAGATCTGGAGAGATATGATCC
TTATCCATTCCTCTGATAACTGCATTCTGAAATTTGAGCATTTGCTCAAT
CAGTTTGAGTCCCGCTTCAAGATGCGCCAATGGGTCCATCATGATTTGAA
GAAGAATAAAAAACTTTTACGAGAGTTAGGAAAGTCAAAGAAATACCGAT
ATTTCCTTGTAGATATTCCTTATTGGCAGATCACGGACTTCTTAATTATG
GCATCCGTTCACAACATGACTGATCCACAGTTTTCTTTTATTTTCACTGA
TTGGGACGCTAGTCTCGTCGATATTGCCGCATTCAAGATCAACAATAAAG
TCAACATCACAACCTTTTCAATTACCGATCATTCGATAATGGAGAGAATG
ACGAAGACCTACGAAAATGTCGAAAAGCTACTGGAAAGTATCGTTGAAAT
GGAGAAGACGAGCAAAAAGTGTGATATAGAAAAAGCATTTATGAGTAAAC
CAGCTATTTTGTTTGATACAGTGAATTTTCTCACGAAAGGTATCATACGT
TTGTTGAAGAGCCGTCCACTAATTGTCCGTCCAATGAAATGCAAATCAAA
CGATACTTGGAGACTAGGTTCAAGTCTGATCAACGCAATGACTGCAGCGG
AAAACTTGTATGGAATAACCGGGAAAATCCTATTCAACGAAAATGCTCAA
AGGAAAAACTACAATCTGTCAATCATGGAGTTAACGGAAAATGGCTTTCA
AAAGGGAGGATTTTGGAATGATGACAGATTTGTCGTCTTGCCGAAACGGA
TTGAACCGTCAGTGGTTGAAATTGGAAATATAGTCCGGCCTCTAAAAGTC
ACGACAATTATAAGCCAGCCGTTCGTATTTTACAAAGAGGGTGAGCCCGA
TCGTCAGAACTACGATACGAATATGGAGGCTTGGAACGGATTTTGCATTG
ATTTGTTGCGAGAAATCGGAAACGACTTGGGAATGAATTTCACGATTCAC
TTGGTGAAGGATAACAAATACGGCGAAGCGGTGGAGAACCAAACGAATCC
TGACGGATCAGAGAAATGGAACGGCATGATAGGAGAACTCATTACCAAGA
GGTTCATTGTAAACCAAATTTCGATTAATTAAAAAAGATGCTCATCAGCT
TCCACATTGAAACATCATGATAAAATTGGTATAATCACCTTTAATAAATT
GTTGAAGAACCTTGAAACTGTGGAGGAATAGTTCAACAAACCTTTGCTAT
TTTTCACTCGATCTAATTTGATGTGAATACCCTTTGTGCGGCTACTTACC
ATTTTATAGCACCTTTCTTCTGGAAGGTTGAAGAGCTCAAATACTTACAG
AAGATTTGCAGTAATATTTGGATTTTATTCAGAGTTTTGGAATTAGTTCT
TTCATTTAATTTTCACTTATTCATAAATACTCAAGCGGAACTTGCAATGG
CTCCGATGACGATTTCTTACGAACGAGAAAAGGTTGTCGATTTCACGACT
CCTTTCATGAGTTTAGGATTAAGTATTTTATTCTTAAAACCGAAAAAAGA
AGCTCCTGGCTTATTTTCATTTCTGTCACCGATATCATTTCCGGTTTGGG
TATATATCATCAGCGCTTACATAGGAGTGAGCTTTATCATCTTCTTTATA
TCCCATGTTACGCCTTATGAATGGCTTCAAATACACCCTTGTGATGATAA
CAGCGAACTAGACAATCAGTTCAATTTATTAAACAGTTTATGGTTTACTG
TTGGAGCCTTGGTGCGACAAGGTGAAATGTGGCAAGTTGTATTGATAATG
CACATGTGTAGTGATAGGTCAAATTTCATAAAATGTCTTCAATAATATTT
ATTCAGCCAAATATTTCAACTTCTTATTAGAGTCTTTTGATACTGTAATG
TGAGAACCATCAGCGATAAAACTTATTCGTTTATCCGGCACAATCTGGCC
TGAGCATTGTTGGCTATGCGAAAATGTTGGATAAACCTTCTTAACCCATA
TATTAAATAAATTGTTTTTGTTCATCAGAATCAGTTGAAGATGTTCTCTT
TTCATTGACATTGGGCAAGTAAAGCCTATAAACTTTAGATATATAAAAAA
AGTAATTATTTATTTATTAATTATTAATTTTCATTTTTTTGTCGGATAAA
CCGGATGCCGAATAATCGAGAGTTGATTAATTGAAGTATTGCTGTTGTGA
CATTATTTACTGGTTATTCTTGCCACTCAATCCCAGATAAAGAGAGGAGT
GAACTTCGAATTTAGTCCTTCAAAATAGGATTTTGCTTTGTTGAATTTGA
GTACCCATCGATGGCAGCTGCATCATAGCAATGCCAAGGCTCTGAACTGT
ATCTCTCTTTCATGTGCCCCATTTCTACTGCAATACTGATATCCATAATC
TTAATTTTCATTAGAATCTGATATTCGTATAGACTTGACCTCGATATGGC
TTTGAATTTCACGATGAATGAATTCAAGCATACACAAAATTGCATATATA
TAGTCTCTTGAATTGTACTCATAGGATTTTAAGCTTTCTTTACCTTTTAA
CCTTTTGAAACTCGTTTTTGTCATCAATAATTGAAATGAAAAACACGAAA
AATTAAATTTATTATTGAAGATATTTTCGGGTCGAACAAATGGTAATTGG
GATAGCGTTAGCCCCCGCTCATTTTGGTAATGTGTATTTTCCACGTCATT
GATTGAAAGGGTTTTTTTTCGAAAAGGTAAGTGGACAGCTGAAACATTTA
ATCTTGTAGCATTGATCTCAAACAGCAAGACGTAGATTGTTTTTTTACAT
TTGGAAAATCTGAATTTAAATCGAGCTCATGAAAATGATCGGATCTTGTC
TGTGATGTGTTTCCGTTGCATGGTTGTGCATGCCAGTAGATTTTGTCTGC
AATAATTTCATTTCTTTATAATTATTATTATTAATATTCATTGCTTCTAT
AGGTCTTGAATTTTGTCCCCAAGCCTCATCAACTAGAATGGTATCGGGAG
CTTGGTGGTTCTTCACTCTCATCATCATTTCTACATACACTGCCAACCTG
GCGGCTTTCCTTACTGTCGAGCAGTTAATTCTTCCGATTAAAAGCGTTGA
GGATCTCGCACAACAAACGAACATCAAATACGGAACACTAAAGGGAGGAA
CATCGTTCACCTTTTTTGAGAAAGCTCTCATCCCGACCTATCAAAAAATG
TTCAAATTCATGAAAAAGAACAGAGAGGTCTTCGTCGATTCAACTGAAAA
AGGAGTTAATAAAGTTCGTAATTCAAATGGAAAATACGCTTTTCTTCTTG
AGTCAGCCATGAATGATTTTTATTCTCAACGGGATTGTAGCTTGATGAGA
ATAGGAGACTTATTAGATTCCAAGGGCTATGGAATTGGATTCCCATCAGG
TTCGCCGTTGCGAGATCATATGACTAAATCTATCCTAAAGTTGCAAAAGT
CCCAAAAAATAGCCAATCTCATTCACAAATGGTGGAATGAAATGAATATC
ACCGAACCTTGTGATTCCAAAGAATCGGGTTCAGTGCCAACTGAGCTAAA
CGTATACCAAATCGGGGGGATTTTTATTTTATTAGTTGGAGGATTTATAT
TTGCCGCCATTGTGTCGATCATCGAGCTCTACGTCGACTACTGCAACAAG
AAATCTCAGTGGAAGATCCCATTTGGCAAATACCTAAAGAAGGAACTTCA
ATTCGCTATGAGATTTATGAGTTCATCCAAGAAGCCGCTCCATCCAGATC
TCACTAATAGCAAGTCCCGAGAACGACTGAATTTGCTAGATCATCAATCT
GGAAATGTAAATGATGTCTCATTGGAAGCTTCTCGCAACATGAGCAATTT
TACTAGCAAGTCTGAAAGTCACCAAAGCGGTCTCAATCGCCGGCAAATCT
TCACGGACATTGACAGTCCTTGCCTGGACCGCAGTCTTGGCTTCGATAGT
TCTCATGACGGATTGAGCAGTATTCCAAATTCAAAACCTTTATGGGGCTT
GAATAGCAATTTGTGATTAGATTTTCCAGCGCTCAGATTTTGCTGCATGG
CCGGGATAGGGCATTCAGAACTTTGTTTATCATTTCTGTTAGAAGCGCCG
AAAACATCCTTTGTCAAATCTTTATTCTTGATTGGGAAACTTTAAAAATC
CTTTATTAAAT
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protein sequence of SMED30003453-orf-1

>SMED30003453-orf-1 ID=SMED30003453-orf-1|Name=SMED30003453-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=133bp
MAPMTISYEREKVVDFTTPFMSLGLSILFLKPKKEAPGLFSFLSPISFPV
WVYIISAYIGVSFIIFFISHVTPYEWLQIHPCDDNSELDNQFNLLNSLWF
TVGALVRQGEMWQVVLIMHMCSDRSNFIKCLQ*
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protein sequence of SMED30003453-orf-2

>SMED30003453-orf-2 ID=SMED30003453-orf-2|Name=SMED30003453-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=343bp
MVSGAWWFFTLIIISTYTANLAAFLTVEQLILPIKSVEDLAQQTNIKYGT
LKGGTSFTFFEKALIPTYQKMFKFMKKNREVFVDSTEKGVNKVRNSNGKY
AFLLESAMNDFYSQRDCSLMRIGDLLDSKGYGIGFPSGSPLRDHMTKSIL
KLQKSQKIANLIHKWWNEMNITEPCDSKESGSVPTELNVYQIGGIFILLV
GGFIFAAIVSIIELYVDYCNKKSQWKIPFGKYLKKELQFAMRFMSSSKKP
LHPDLTNSKSRERLNLLDHQSGNVNDVSLEASRNMSNFTSKSESHQSGLN
RRQIFTDIDSPCLDRSLGFDSSHDGLSSIPNSKPLWGLNSNL*
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protein sequence of SMED30003453-orf-3

>SMED30003453-orf-3 ID=SMED30003453-orf-3|Name=SMED30003453-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=520bp
MLIFGFVIFFLSICPISTKELSKKLLNIGVILDASDDISRKVISLAVQNA
NKELARFNYAFQVKTETLDDNNSFYAIRKGCNLIRHGLVAMISPIAEVPS
RHISSLCSKFHIPHLVTSCRFPESLDRYPMDIQLTPNCDQYAKMIYQYIT
QVEIWRDMILIHSSDNCILKFEHLLNQFESRFKMRQWVHHDLKKNKKLLR
ELGKSKKYRYFLVDIPYWQITDFLIMASVHNMTDPQFSFIFTDWDASLVD
IAAFKINNKVNITTFSITDHSIMERMTKTYENVEKLLESIVEMEKTSKKC
DIEKAFMSKPAILFDTVNFLTKGIIRLLKSRPLIVRPMKCKSNDTWRLGS
SLINAMTAAENLYGITGKILFNENAQRKNYNLSIMELTENGFQKGGFWND
DRFVVLPKRIEPSVVEIGNIVRPLKVTTIISQPFVFYKEGEPDRQNYDTN
MEAWNGFCIDLLREIGNDLGMNFTIHLVKDNKYGEAVENQTNPDGSEKWN
GMIGELITKRFIVNQISIN*
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protein sequence of SMED30003453-orf-4

>SMED30003453-orf-4 ID=SMED30003453-orf-4|Name=SMED30003453-orf-4|organism=Schmidtea mediterranea sexual|type=polypeptide|length=122bp
MRAFSKKVNDVPPFSVPYLMFVCCARSSTLLIGRINCSTVRKAARLAVYV
EMMMRVKNHQAPDTILVDEAWGQNSRPIEAMNINNNNYKEMKLLQTKSTG
MHNHATETHHRQDPIIFMSSI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0015276ligand-gated ion channel activity
GO:0038023signaling receptor activity
GO:0005234extracellular-glutamate-gated ion channel activity
GO:0004970ionotropic glutamate receptor activity
GO:0004872receptor activity
GO:0005216ion channel activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0003104enteric muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR019594Glu/Gly-bd
IPR001320Iontro_rcpt
IPR001508Iono_rcpt_met
IPR001638Solute-binding_3/MltF_N
IPR001828ANF_lig-bd_rcpt
IPR028082Peripla_BP_I
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0005886plasma membrane
GO:0016021integral component of membrane
GO:0030054cell junction
GO:0045202synapse
GO:0045211postsynaptic membrane
Vocabulary: biological process
TermDefinition
GO:0006811ion transport
GO:0006810transport
GO:0034220ion transmembrane transport
GO:0035235ionotropic glutamate receptor signaling pathway
GO:0060079excitatory postsynaptic potential
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 273..293
NoneNo IPR availableGENE3DG3DSA:3.40.50.2300coord: 137..401
e-value: 4.1E-69
score: 235.5
NoneNo IPR availableGENE3DG3DSA:3.40.190.10coord: 422..519
e-value: 1.7E-20
score: 75.2
NoneNo IPR availableGENE3DG3DSA:3.40.50.2300coord: 42..380
e-value: 4.1E-69
score: 235.5
NoneNo IPR availablePANTHERPTHR18966IONOTROPIC GLUTAMATE RECEPTORcoord: 142..508
NoneNo IPR availablePANTHERPTHR18966:SF370coord: 142..508
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.713
NoneNo IPR availableSUPERFAMILYSSF53850Periplasmic binding protein-like IIcoord: 423..511
IPR019594Ionotropic glutamate receptor, L-glutamate and glycine-binding domainSMARTSM00918Lig_chan_Glu_bd_2coord: 433..508
e-value: 6.3E-18
score: 75.6
IPR019594Ionotropic glutamate receptor, L-glutamate and glycine-binding domainPFAMPF10613Lig_chan-Glu_bdcoord: 424..510
e-value: 1.4E-18
score: 67.2
IPR001828Receptor, ligand binding regionPFAMPF01094ANF_receptorcoord: 43..391
e-value: 6.3E-29
score: 101.2
IPR028082Periplasmic binding protein-like ISUPERFAMILYSSF53822Periplasmic binding protein-like Icoord: 25..399