Glycine N-methyltransferase

Overview
NameGlycine N-methyltransferase
Smed IDSMED30003448
Length (bp)1103
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Glycine N-methyltransferase (SMED30003448) t-SNE clustered cells

Violin plots show distribution of expression levels for Glycine N-methyltransferase (SMED30003448) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Glycine N-methyltransferase (SMED30003448) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Glycine N-methyltransferase (SMED30003448) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003448

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30003448SMESG000037719.1 SmedASXL_008095SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult colorimetric in situ hybridization evidence
pigment cellSMED30003448SMESG000037719.1 SmedASXL_008095SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
gutSMED30003448SMESG000037719.1 dd_Smed_v4_1127_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003448SMESG000037719.1 dd_Smed_v4_1127_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30003448SMESG000037719.1 dd_Smed_v4_1127_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003448SMESG000037719.1 Contig36779newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003448SMESG000037719.1 Contig36779uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003448SMESG000037719.1 Contig23824newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003448SMESG000037719.1 Contig23824uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003448SMESG000037719.1 Contig48865newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003448SMESG000037719.1 Contig48865uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Glycine N-methyltransferase vs. Ensembl Human
Match: GNMT (glycine N-methyltransferase [Source:HGNC Symbol;Acc:HGNC:4415])

HSP 1 Score: 342.813 bits (878), Expect = 4.759e-117
Identity = 180/316 (56.96%), Postives = 217/316 (68.67%), Query Frame = 3 
Query:  150 MVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTN---GFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNK-KEKVYGDFQDLTKIKD--PAYYIHVMTK 1079
            MVD +YRTRSLGVA  GLPDQYADG+AA+VW+LY+G  + RT+ YK W + LL+ H    VLDVACGTGVDS+ML+EEGF+V S DASDKMLK ALKERWNRRHE  FD+WVIEEANW+TL          K +P +   GFDAVICLGNSFAHLPD  GDQSE ++AL N+  ++R  G+LVIDHRNYD+IL TG AP GKN+YY SD  K + TS L V+ K  M+TLDYT+ + G   +      P LS+          FRLSYYPH L  FTELLQ  F  K +  V GDF+     +   P Y+IHV+ +
Sbjct:    1 MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCQRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMTLD---------KDVPQSAEGGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRAGGLLVIDHRNYDHILSTGCAPPGKNIYYKSDLTKDVTTSVLIVNNKAHMVTLDYTVQVPGAGQDGS----PGLSK----------FRLSYYPHCLASFTELLQAAFGGKCQHSVLGDFKPYKPGQTYIPCYFIHVLKR 293
[Close]
BLAST of Glycine N-methyltransferase vs. Ensembl Fly
Match: Gnmt (gene:FBgn0038074 transcript:FBtr0082651)

HSP 1 Score: 309.301 bits (791), Expect = 2.270e-104
Identity = 159/310 (51.29%), Postives = 209/310 (67.42%), Query Frame = 3 
Query:  153 VDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNK-KEKVYGDFQDLTKIKDPAYYIHVMTK 1079
             D ++  RS G++  G+ DQYADGKAAKVWE+++G K  RT  YK + I++L+N     VLDVACGTGVDS+ML+EEGF V+S DASDKMLK ALKERW RR+E  FD+WVIEEANWLTL  D+           +GFDAVICLGNSFAHL D  GDQ E + A+ N  + L+P G+L+IDHRNYD IL+TG  P  K++YY + H   IKTS LF  GKP ++++DY +    + +E                     FRLSYYPH L RF E+L  +F  K K ++YGDF++++++K+PA+YIH++ K
Sbjct:    5 ADSVFVARSDGISAEGVRDQYADGKAAKVWEIFIGDKNSRTDNYKNFLIDMLRNKGCKRVLDVACGTGVDSLMLVEEGFEVVSVDASDKMLKYALKERWARRNEAAFDKWVIEEANWLTLYDDIQEHI------QDGFDAVICLGNSFAHLMDGFGDQREHKQAIGNFEKCLKPGGVLLIDHRNYDNILETGATP-AKSIYYNTSHTADIKTSVLFYCGKPALVSMDYLIAGNKLTSE---------------------FRLSYYPHGLKRFQEILAEIFSAKAKHQLYGDFKEMSQVKNPAFYIHLIEK 286
[Close]
BLAST of Glycine N-methyltransferase vs. Ensembl Zebrafish
Match: gnmt (glycine N-methyltransferase [Source:ZFIN;Acc:ZDB-GENE-040227-1])

HSP 1 Score: 343.584 bits (880), Expect = 1.488e-117
Identity = 181/312 (58.01%), Postives = 219/312 (70.19%), Query Frame = 3 
Query:  150 MVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNKKE-KVYGDFQDLTKIKD-PAYYIHVMTK 1079
            MVD +YRTRSLGV   GLPDQYADGKAAKVW+LY+G  + RT  YK W + LL+ H V  VLDVACGTGVDS+ML+EEGF+V+S DASDKMLK ALKERW RR E  FD WVIEEANWLTL +D+         P +GFDAVICLGNSFAHLPD  GDQS+Q++AL N+  +++P GIL+IDHRNYDYI++TGQ P+GKN+YY SD  + I TS L+V+ KP MITLDYT+          +   P   E  Q       FRLSYYPH ++ F E+L+  F  K E  VY DF+  T   + P Y+IHV+ K
Sbjct:    1 MVDSVYRTRSLGVGAVGLPDQYADGKAAKVWQLYIGDTRSRTEEYKSWVLALLRKHGVQRVLDVACGTGVDSIMLVEEGFSVVSVDASDKMLKYALKERWERRKETAFDNWVIEEANWLTLPEDVKK-------PGDGFDAVICLGNSFAHLPDFKGDQSDQKLALQNIGSMVKPGGILIIDHRNYDYIIETGQVPQGKNIYYKSDLKQDISTSVLWVNSKPHMITLDYTL----------VLPQP---EGAQTPPDTSKFRLSYYPHRVENFKEILKAAFWGKCEHNVYADFKQYTAGGEPPCYFIHVVKK 292
[Close]
BLAST of Glycine N-methyltransferase vs. Ensembl Xenopus
Match: ENSXETT00000024750.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV461671.1:2929:3138:-1 gene:ENSXETG00000015000.1 transcript:ENSXETT00000024750.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 108.612 bits (270), Expect = 5.398e-29
Identity = 45/69 (65.22%), Postives = 54/69 (78.26%), Query Frame = 3 
Query:  150 MVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTG 356
            MVD +YRTRSLGV   GLPDQYADG+AA+VW LY+G K+ RT  Y+ W ++LL+ HN   VLDVACGTG
Sbjct:    1 MVDSVYRTRSLGVGAEGLPDQYADGRAARVWHLYIGDKRSRTDEYRSWILSLLQEHNCRTVLDVACGTG 69
[Close]
BLAST of Glycine N-methyltransferase vs. Ensembl Mouse
Match: Gnmt (glycine N-methyltransferase [Source:MGI Symbol;Acc:MGI:1202304])

HSP 1 Score: 342.813 bits (878), Expect = 2.827e-117
Identity = 178/313 (56.87%), Postives = 214/313 (68.37%), Query Frame = 3 
Query:  150 MVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNK-KEKVYGDFQDLTKIKD--PAYYIHVMTK 1079
            MVD +YRTRSLGVA  GLPDQYADG+AA+VW+LY+G  + RT+ YK W + LL+ H    VLDVACGTGVDS+ML+EEGF+V+S DASDKMLK ALKERWNRR E  FD WVIEEANWLTL +         V+  +GFDAVICLGNSFAHLPD  GDQSE ++AL N+  ++RP G+LVIDHRNYDYIL TG AP GKN+YY SD  K I TS L V+ K  M+TLDYT+ + G   +                  F  FRLSYYPH L  FTEL++  F  + +  V GDF+     +   P Y+IHV+ K
Sbjct:    1 MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCHRVLDVACGTGVDSIMLVEEGFSVMSVDASDKMLKYALKERWNRRKEPSFDNWVIEEANWLTLDK--------DVLSGDGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRPGGLLVIDHRNYDYILSTGCAPPGKNIYYKSDLTKDITTSVLTVNNKAHMVTLDYTVQVPGTGRDGSP--------------GFSKFRLSYYPHCLASFTELVRAAFGGRCQHSVLGDFKPYKPGQAYVPCYFIHVLKK 291
[Close]
BLAST of Glycine N-methyltransferase vs. UniProt/SwissProt
Match: sp|Q9QXF8|GNMT_MOUSE (Glycine N-methyltransferase OS=Mus musculus OX=10090 GN=Gnmt PE=1 SV=3)

HSP 1 Score: 342.813 bits (878), Expect = 1.977e-116
Identity = 178/313 (56.87%), Postives = 214/313 (68.37%), Query Frame = 3 
Query:  150 MVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNK-KEKVYGDFQDLTKIKD--PAYYIHVMTK 1079
            MVD +YRTRSLGVA  GLPDQYADG+AA+VW+LY+G  + RT+ YK W + LL+ H    VLDVACGTGVDS+ML+EEGF+V+S DASDKMLK ALKERWNRR E  FD WVIEEANWLTL +         V+  +GFDAVICLGNSFAHLPD  GDQSE ++AL N+  ++RP G+LVIDHRNYDYIL TG AP GKN+YY SD  K I TS L V+ K  M+TLDYT+ + G   +                  F  FRLSYYPH L  FTEL++  F  + +  V GDF+     +   P Y+IHV+ K
Sbjct:    1 MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCHRVLDVACGTGVDSIMLVEEGFSVMSVDASDKMLKYALKERWNRRKEPSFDNWVIEEANWLTLDK--------DVLSGDGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRPGGLLVIDHRNYDYILSTGCAPPGKNIYYKSDLTKDITTSVLTVNNKAHMVTLDYTVQVPGTGRDGSP--------------GFSKFRLSYYPHCLASFTELVRAAFGGRCQHSVLGDFKPYKPGQAYVPCYFIHVLKK 291
[Close]
BLAST of Glycine N-methyltransferase vs. UniProt/SwissProt
Match: sp|Q14749|GNMT_HUMAN (Glycine N-methyltransferase OS=Homo sapiens OX=9606 GN=GNMT PE=1 SV=3)

HSP 1 Score: 342.813 bits (878), Expect = 2.285e-116
Identity = 180/316 (56.96%), Postives = 217/316 (68.67%), Query Frame = 3 
Query:  150 MVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTN---GFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNK-KEKVYGDFQDLTKIKD--PAYYIHVMTK 1079
            MVD +YRTRSLGVA  GLPDQYADG+AA+VW+LY+G  + RT+ YK W + LL+ H    VLDVACGTGVDS+ML+EEGF+V S DASDKMLK ALKERWNRRHE  FD+WVIEEANW+TL          K +P +   GFDAVICLGNSFAHLPD  GDQSE ++AL N+  ++R  G+LVIDHRNYD+IL TG AP GKN+YY SD  K + TS L V+ K  M+TLDYT+ + G   +      P LS+          FRLSYYPH L  FTELLQ  F  K +  V GDF+     +   P Y+IHV+ +
Sbjct:    1 MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCQRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMTLD---------KDVPQSAEGGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRAGGLLVIDHRNYDHILSTGCAPPGKNIYYKSDLTKDVTTSVLIVNNKAHMVTLDYTVQVPGAGQDGS----PGLSK----------FRLSYYPHCLASFTELLQAAFGGKCQHSVLGDFKPYKPGQTYIPCYFIHVLKR 293
[Close]
BLAST of Glycine N-methyltransferase vs. UniProt/SwissProt
Match: sp|P13255|GNMT_RAT (Glycine N-methyltransferase OS=Rattus norvegicus OX=10116 GN=Gnmt PE=1 SV=2)

HSP 1 Score: 341.273 bits (874), Expect = 7.562e-116
Identity = 179/314 (57.01%), Postives = 214/314 (68.15%), Query Frame = 3 
Query:  150 MVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPT-NGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNK-KEKVYGDFQDLTKIKD--PAYYIHVMTK 1079
            MVD +YRTRSLGVA  G+PDQYADG+AA+VW+LY+G  + RT+ YK W + LL+ H    VLDVACGTGVDS+ML+EEGF+V S DASDKMLK ALKERWNRR E  FD+WVIEEANWLTL          K +P  +GFDAVICLGNSFAHLPD  GDQSE ++AL N+  ++RP G+LVIDHRNYDYIL TG AP GKN+YY SD  K I TS L V+ K  M+TLDYT+ + G   +                  F  FRLSYYPH L  FTEL+Q  F  + +  V GDF+     +   P Y+IHV+ K
Sbjct:    1 MVDSVYRTRSLGVAAEGIPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCHRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRKEPAFDKWVIEEANWLTLD---------KDVPAGDGFDAVICLGNSFAHLPDSKGDQSEHRLALKNIASMVRPGGLLVIDHRNYDYILSTGCAPPGKNIYYKSDLTKDITTSVLTVNNKAHMVTLDYTVQVPGAGRDGAP--------------GFSKFRLSYYPHCLASFTELVQEAFGGRCQHSVLGDFKPYRPGQAYVPCYFIHVLKK 291
[Close]
BLAST of Glycine N-methyltransferase vs. UniProt/SwissProt
Match: sp|Q29555|GNMT_PIG (Glycine N-methyltransferase (Fragment) OS=Sus scrofa OX=9823 GN=GNMT PE=2 SV=1)

HSP 1 Score: 339.732 bits (870), Expect = 3.711e-115
Identity = 176/309 (56.96%), Postives = 209/309 (67.64%), Query Frame = 3 
Query:  162 IYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVF-QNKKEKVYGDFQDLTKIKD--PAYYIHVMTK 1079
            +YRTRSLGVA  GLPDQYADG+AA+VW+LY+G  + RT+ YK W + LL+ H    VLDVACGTGVDS+ML+EEGF+V S DASDKMLK ALKERWNRRHE  FD+WVIEEA          + + PK  P  GFDAVIC GNSFAHLPD  GDQSE ++AL N+  ++R  G+LVIDHRNYD+IL TG AP GKN+YY SD IK I TS L V+ K  M+TLDYT+ + G          P LS+          FRLSYYPH L  FTELLQ  F  N +  V GDF+     +   P Y+IHV+ K
Sbjct:    2 VYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCQRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEA-----NWMTLDKDVPKS-PMGGFDAVICQGNSFAHLPDCRGDQSEHRLALKNITSMVRSGGLLVIDHRNYDHILSTGCAPPGKNIYYKSDLIKDITTSVLTVNNKAHMVTLDYTVQVPGTGHRGS----PGLSK----------FRLSYYPHCLASFTELLQTAFGGNGQHSVLGDFKPYKPGQAYIPCYFIHVLRK 290
[Close]
BLAST of Glycine N-methyltransferase vs. UniProt/SwissProt
Match: sp|Q29513|GNMT_RABIT (Glycine N-methyltransferase (Fragment) OS=Oryctolagus cuniculus OX=9986 GN=GNMT PE=2 SV=1)

HSP 1 Score: 323.168 bits (827), Expect = 1.008e-108
Identity = 175/314 (55.73%), Postives = 211/314 (67.20%), Query Frame = 3 
Query:  156 DRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIP---TNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNK-KEKVYGDFQDLTKIKD--PAYYIHVMTK 1079
            D +YRTRSLGVA  GLPDQYADG+AA+VW+LY+G  + RTS YK W + LL+ H    VLDVACGTGVDS+ML+EEGF+V S DASDKMLK ALKERWNRRHE  FD+WVIEEANW+TL          K +P     GFDAVICLGNSFAHLPD  GDQSE + AL N+  ++R  G+LVIDHRNYD+IL T  AP GKN+YY SD  K + TS L V+ K  M+TLDYT+ +     +      P LS+          FRLSYYPH L  FTE L+  F+ K +  V GDF+     +   P Y+IHV+ +
Sbjct:    1 DSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTSEYKTWLLGLLRQHGCRRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMTLD---------KDVPQPAGGGFDAVICLGNSFAHLPDCKGDQSEHRQALKNIASMVRTGGLLVIDHRNYDHILSTLCAP-GKNIYYKSDLTKDVTTSVLTVNNKAHMVTLDYTVQVPAAGQDGP----PSLSK----------FRLSYYPHCLAPFTEFLRAAFEGKCQHSVLGDFKPYQPGQAYVPCYFIHVLKR 290
[Close]
BLAST of Glycine N-methyltransferase vs. TrEMBL
Match: H3AJK9 (Glycine N-methyltransferase OS=Latimeria chalumnae OX=7897 GN=GNMT PE=3 SV=1)

HSP 1 Score: 363.229 bits (931), Expect = 5.519e-122
Identity = 181/313 (57.83%), Postives = 226/313 (72.20%), Query Frame = 3 
Query:  150 MVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNKKE-KVYGDFQDLTKIKD--PAYYIHVMTK 1079
            MVD +YRTRSLGVA  GLPDQYADGKAA+VW+LY+G  + RT  Y+ W   LLK H    VLDVACGTGVDS+M+LEEGF+V+S DASDKMLK ALKERW+RR E+ FD+WVIEEA            + PK  P +GFDAVICLGNSFAHLPD  GDQS+Q++ALAN+  +++P GIL+IDHRNYDYIL+TG+AP GKN+YY SD  K I TS L+V+G P MITLDYT+ +              + E E+ + +   FRLSYYPH LD FTELL++ F+ K E +V+GDF+   + ++  P Y+IHV+ K
Sbjct:    1 MVDSVYRTRSLGVAAEGLPDQYADGKAARVWQLYIGDTRSRTDEYRNWLRALLKEHGCQQVLDVACGTGVDSIMMLEEGFSVVSVDASDKMLKYALKERWDRRKEEAFDKWVIEEA-----NWLTLMRDVPK--PKDGFDAVICLGNSFAHLPDFKGDQSDQKLALANIAGMVKPGGILIIDHRNYDYILQTGRAPPGKNIYYKSDLTKDITTSVLWVNGNPHMITLDYTVQVP-------------VGEVEEQQPEMSKFRLSYYPHRLDAFTELLKSTFKGKCEHRVFGDFKPYKRNQEYVPCYFIHVLQK 293
[Close]
BLAST of Glycine N-methyltransferase vs. TrEMBL
Match: A0A3P9L7D3 (Glycine N-methyltransferase OS=Oryzias latipes OX=8090 PE=3 SV=1)

HSP 1 Score: 361.688 bits (927), Expect = 1.979e-121
Identity = 190/314 (60.51%), Postives = 228/314 (72.61%), Query Frame = 3 
Query:  147 IMVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNKKE-KVYGDFQDLT--KIKDPAYYIHVMTK 1079
            + VD ++RTRSLGVA  GLPDQYADGKAAKVWELY+G  Q RT  YK W ++LLK H V  VLDVACGTGVDS+ML+EEGFN++S DASDKMLK ALK RW+RR E  FDQWVIEEANWLTLQ D++        P +G+DAVICLGNSFAHLPD  GDQSEQ++AL N+  ++RP GIL+IDHRNYDYIL+TG+AP+GKN+YY SD  + I TS L+V+ KP MITLDYT+ +     + +   LP +S+          FRLSYYPH LD F ELL+  F  K E  VYGDFQ     + + P Y+IHV  K
Sbjct:    1 MSVDSVFRTRSLGVAAEGLPDQYADGKAAKVWELYIGDTQSRTQEYKSWVVSLLKQHGVRTVLDVACGTGVDSIMLVEEGFNMVSVDASDKMLKYALKARWDRRKESAFDQWVIEEANWLTLQADIHK-------PADGYDAVICLGNSFAHLPDFKGDQSEQKLALHNIASMVRPGGILIIDHRNYDYILETGRAPQGKNIYYKSDLTQDITTSVLWVNSKPHMITLDYTIHV----PQAEHNNLPEVSK----------FRLSYYPHRLDNFRELLKEAFSGKMEHSVYGDFQTYVPGRSQPPCYFIHVCKK 293
[Close]
BLAST of Glycine N-methyltransferase vs. TrEMBL
Match: A0A3B3H9X6 (Glycine N-methyltransferase OS=Oryzias latipes OX=8090 GN=gnmt PE=3 SV=1)

HSP 1 Score: 361.688 bits (927), Expect = 1.979e-121
Identity = 190/314 (60.51%), Postives = 228/314 (72.61%), Query Frame = 3 
Query:  147 IMVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNKKE-KVYGDFQDLT--KIKDPAYYIHVMTK 1079
            + VD ++RTRSLGVA  GLPDQYADGKAAKVWELY+G  Q RT  YK W ++LLK H V  VLDVACGTGVDS+ML+EEGFN++S DASDKMLK ALK RW+RR E  FDQWVIEEANWLTLQ D++        P +G+DAVICLGNSFAHLPD  GDQSEQ++AL N+  ++RP GIL+IDHRNYDYIL+TG+AP+GKN+YY SD  + I TS L+V+ KP MITLDYT+ +     + +   LP +S+          FRLSYYPH LD F ELL+  F  K E  VYGDFQ     + + P Y+IHV  K
Sbjct:    1 MSVDSVFRTRSLGVAAEGLPDQYADGKAAKVWELYIGDTQSRTQEYKSWVVSLLKQHGVRTVLDVACGTGVDSIMLVEEGFNMVSVDASDKMLKYALKARWDRRKESAFDQWVIEEANWLTLQADIHK-------PADGYDAVICLGNSFAHLPDFKGDQSEQKLALHNIASMVRPGGILIIDHRNYDYILETGRAPQGKNIYYKSDLTQDITTSVLWVNSKPHMITLDYTIHV----PQAEHNNLPEVSK----------FRLSYYPHRLDNFRELLKEAFSGKMEHSVYGDFQTYVPGRSQPPCYFIHVCKK 293
[Close]
BLAST of Glycine N-methyltransferase vs. TrEMBL
Match: A0A3P9JEI3 (Glycine N-methyltransferase OS=Oryzias latipes OX=8090 PE=3 SV=1)

HSP 1 Score: 361.303 bits (926), Expect = 2.900e-121
Identity = 190/314 (60.51%), Postives = 228/314 (72.61%), Query Frame = 3 
Query:  147 IMVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNKKE-KVYGDFQDLT--KIKDPAYYIHVMTK 1079
            + VD ++RTRSLGVA  GLPDQYADGKAAKVWELY+G  Q RT  YK W ++LLK H V  VLDVACGTGVDS+ML+EEGFN++S DASDKMLK ALK RW+RR E  FDQWVIEEANWLTLQ D++        P +G+DAVICLGNSFAHLPD  GDQSEQ++AL N+  ++RP GIL+IDHRNYDYIL+TG+AP+GKN+YY SD  + I TS L+V+ KP MITLDYT+ +     + +   LP +S+          FRLSYYPH LD F ELL+  F  K E  VYGDFQ     + + P Y+IHV  K
Sbjct:    1 MSVDSVFRTRSLGVAAEGLPDQYADGKAAKVWELYIGDTQSRTQEYKSWVVSLLKQHGVRTVLDVACGTGVDSIMLVEEGFNMVSVDASDKMLKYALKARWDRRKESAFDQWVIEEANWLTLQADIHK-------PADGYDAVICLGNSFAHLPDFKGDQSEQKLALHNIASMVRPGGILIIDHRNYDYILETGRAPQGKNIYYKSDLTQDITTSVLWVNSKPHMITLDYTIHV----PQAEHNNLPEVSK----------FRLSYYPHRLDNFRELLKEAFSCKMEHSVYGDFQTYVPGRSQPPCYFIHVCKK 293
[Close]
BLAST of Glycine N-methyltransferase vs. TrEMBL
Match: A0A1A8F5H2 (Glycine N-methyltransferase OS=Nothobranchius korthausae OX=1143690 GN=GNMT PE=3 SV=1)

HSP 1 Score: 361.303 bits (926), Expect = 3.234e-121
Identity = 185/314 (58.92%), Postives = 232/314 (73.89%), Query Frame = 3 
Query:  147 IMVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNKKE-KVYGDFQDLTKIKD--PAYYIHVMTK 1079
            + VD ++RTRSLGVA  GLPDQYADGKAAKVWELY+G  Q RT  YK W ++LL+ H V  VLDVACGTGVDS+ML+EEGFN++S DASDKMLK ALK RWNRR E  FDQWVIEEANWLTL +D+         P +GFDAVICLGNSFAHLPD  GDQS+Q++AL N+  ++RP GIL+IDHRNYDYIL+TG+AP+GKN+YY SD  + I TS L+V+ KP MITLDYT+ +     + ++ KLP +S+          FRLSYYPH L+ F++LL + F  K E +VYGDF++    ++  P Y+IH+  +
Sbjct:    1 MSVDSVFRTRSLGVAAEGLPDQYADGKAAKVWELYIGDTQSRTQEYKSWLVSLLRQHGVRRVLDVACGTGVDSIMLVEEGFNMVSVDASDKMLKYALKARWNRRKEAAFDQWVIEEANWLTLPEDVQK-------PGDGFDAVICLGNSFAHLPDFKGDQSDQKLALHNIGSMVRPGGILIIDHRNYDYILETGKAPQGKNIYYKSDLTQDISTSVLWVNSKPHMITLDYTIQV----PQAELQKLPEVSK----------FRLSYYPHRLESFSQLLMDAFGGKMEHRVYGDFKEYVPGQNQAPCYFIHICKR 293
[Close]
BLAST of Glycine N-methyltransferase vs. Ensembl Cavefish
Match: gnmt (glycine N-methyltransferase [Source:ZFIN;Acc:ZDB-GENE-040227-1])

HSP 1 Score: 311.997 bits (798), Expect = 1.554e-105
Identity = 163/294 (55.44%), Postives = 204/294 (69.39%), Query Frame = 3 
Query:  207 DQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNKKEK-VYGDFQDLTKIKD--PAYYIHVMTK 1079
            D YADGKAAKVW+LY+G  + RT  Y+ W   LL+ HN   VLDVACGTGVDS+ML+EEGF + S DASDKMLK ALKERW RR E  FDQWVIEEANWLTL +D+         P +GFDAVICLGNSFAHLPD  GDQS+Q++AL N+  +++P GIL+IDHRNYDYIL+TG+AP+GKN+YY SD  + I TS L+V+ KP M+TLDY++++               +E      +   FRLSYYPH L+ F ELL+  F  K E+ V+GDF+     +   P Y+IHV+ K
Sbjct:    3 DSYADGKAAKVWQLYIGDTRRRTDEYRSWLAGLLRQHNCIRVLDVACGTGVDSIMLVEEGFKMFSVDASDKMLKYALKERWERRKEPAFDQWVIEEANWLTLSEDVQK-------PGDGFDAVICLGNSFAHLPDFKGDQSDQKLALCNIASMVKPGGILIIDHRNYDYILETGRAPEGKNIYYQSDLKQDISTSVLWVNSKPHMVTLDYSLEVP-------------QAEGAHTPPETSKFRLSYYPHQLESFKELLKEAFLGKCEQSVFGDFKQHKPGQGDAPRYFIHVVKK 276
[Close]
BLAST of Glycine N-methyltransferase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000010933.1 (pep scaffold:Pmarinus_7.0:GL476423:348413:348622:1 gene:ENSPMAG00000009906.1 transcript:ENSPMAT00000010933.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 102.834 bits (255), Expect = 8.654e-28
Identity = 42/69 (60.87%), Postives = 51/69 (73.91%), Query Frame = 3 
Query:  150 MVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTG 356
            MVD ++RTRSLGV   GLPDQYADG+AA+VW+LY+G  Q RT  Y+ W   LL +H    +LDVACGTG
Sbjct:    1 MVDNVFRTRSLGVPAEGLPDQYADGRAARVWQLYIGDTQSRTDEYRAWLTKLLSDHGCKRILDVACGTG 69
[Close]
BLAST of Glycine N-methyltransferase vs. Ensembl Nematostella
Match: EDO33266 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SSQ7])

HSP 1 Score: 328.176 bits (840), Expect = 6.569e-112
Identity = 170/310 (54.84%), Postives = 216/310 (69.68%), Query Frame = 3 
Query:  153 VDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNKKEKV-YGDFQDLTKIKDPAYYIHVMTK 1079
            +D +YRTRSLGV  +G+PDQYADGKAAKVW+ Y+G  ++RT  Y+E+F NLL+  N+ NVLDV+CGTGVDS+MLLE GF V S DASDKMLK AL+ RWNRR E+ FD+WVIEE NWL L             P  GFD +ICLGNSFAHLPD  GD + Q+VA+ N    L+P G L+IDHRNYD I+ TG+AP  KN+YY S+ I+ IKTS L VDG+ TM+TLDYT+D +    E++       SE E        FRLSY PH L  F+ LL+++F    E + +GDF+ L KI++PAYY+H++ K
Sbjct:    1 MDGVYRTRSLGVPATGIPDQYADGKAAKVWQHYIGGHKKRTESYREFFCNLLRERNIHNVLDVSCGTGVDSIMLLENGFCVTSVDASDKMLKDALRIRWNRRKEEPFDKWVIEEGNWLYLDDADIEP------PEGGFDGIICLGNSFAHLPDFNGDLANQRVAMTNFMNFLKPGGWLIIDHRNYDAIIDTGKAPS-KNLYYNSECIEDIKTSVLLVDGRATMVTLDYTVDTSKANIEKE-------SESEP-------FRLSYCPHRLANFSNLLKDIFGKNAEHITFGDFKPLGKIENPAYYVHLIQK 289
[Close]
BLAST of Glycine N-methyltransferase vs. Ensembl Medaka
Match: gnmt (glycine N-methyltransferase [Source:NCBI gene;Acc:101154832])

HSP 1 Score: 361.688 bits (927), Expect = 7.450e-125
Identity = 190/314 (60.51%), Postives = 228/314 (72.61%), Query Frame = 3 
Query:  147 IMVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYTMDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNVFQNKKE-KVYGDFQDLT--KIKDPAYYIHVMTK 1079
            + VD ++RTRSLGVA  GLPDQYADGKAAKVWELY+G  Q RT  YK W ++LLK H V  VLDVACGTGVDS+ML+EEGFN++S DASDKMLK ALK RW+RR E  FDQWVIEEANWLTLQ D++        P +G+DAVICLGNSFAHLPD  GDQSEQ++AL N+  ++RP GIL+IDHRNYDYIL+TG+AP+GKN+YY SD  + I TS L+V+ KP MITLDYT+ +     + +   LP +S+          FRLSYYPH LD F ELL+  F  K E  VYGDFQ     + + P Y+IHV  K
Sbjct:    1 MSVDSVFRTRSLGVAAEGLPDQYADGKAAKVWELYIGDTQSRTQEYKSWVVSLLKQHGVRTVLDVACGTGVDSIMLVEEGFNMVSVDASDKMLKYALKARWDRRKESAFDQWVIEEANWLTLQADIHK-------PADGYDAVICLGNSFAHLPDFKGDQSEQKLALHNIASMVRPGGILIIDHRNYDYILETGRAPQGKNIYYKSDLTQDITTSVLWVNSKPHMITLDYTIHV----PQAEHNNLPEVSK----------FRLSYYPHRLDNFRELLKEAFSGKMEHSVYGDFQTYVPGRSQPPCYFIHVCKK 293
[Close]
BLAST of Glycine N-methyltransferase vs. Planmine SMEST
Match: SMESG000037719.1 (SMESG000037719.1)

HSP 1 Score: 451.825 bits (1161), Expect = 2.752e-161
Identity = 231/232 (99.57%), Postives = 232/232 (100.00%), Query Frame = 3 
Query:   90 MTATQAENSVSSKNFTNQDIMVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEAXXXXXXXXXXXXXXPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKIK 785
            MTATQAENSVSSKNFTNQDIMVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEANWLTLQQDLNNLNNPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKK+K
Sbjct:    1 MTATQAENSVSSKNFTNQDIMVDRIYRTRSLGVAVSGLPDQYADGKAAKVWELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGFNVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEANWLTLQQDLNNLNNPKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILVIDHRNYDYILKTGQAPKGKNVYYPSDHIKKLK 232
The following BLAST results are available for this feature:
BLAST of Glycine N-methyltransferase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
GNMT4.759e-11756.96glycine N-methyltransferase [Source:HGNC Symbol;Ac... [more]
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BLAST of Glycine N-methyltransferase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycine N-methyltransferase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Gnmt2.270e-10451.29gene:FBgn0038074 transcript:FBtr0082651[more]
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BLAST of Glycine N-methyltransferase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gnmt1.488e-11758.01glycine N-methyltransferase [Source:ZFIN;Acc:ZDB-G... [more]
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BLAST of Glycine N-methyltransferase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSXETT00000024750.15.398e-2965.22pep primary_assembly:Xenopus_tropicalis_v9.1:KV461... [more]
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BLAST of Glycine N-methyltransferase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Gnmt2.827e-11756.87glycine N-methyltransferase [Source:MGI Symbol;Acc... [more]
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BLAST of Glycine N-methyltransferase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9QXF8|GNMT_MOUSE1.977e-11656.87Glycine N-methyltransferase OS=Mus musculus OX=100... [more]
sp|Q14749|GNMT_HUMAN2.285e-11656.96Glycine N-methyltransferase OS=Homo sapiens OX=960... [more]
sp|P13255|GNMT_RAT7.562e-11657.01Glycine N-methyltransferase OS=Rattus norvegicus O... [more]
sp|Q29555|GNMT_PIG3.711e-11556.96Glycine N-methyltransferase (Fragment) OS=Sus scro... [more]
sp|Q29513|GNMT_RABIT1.008e-10855.73Glycine N-methyltransferase (Fragment) OS=Oryctola... [more]
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BLAST of Glycine N-methyltransferase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
H3AJK95.519e-12257.83Glycine N-methyltransferase OS=Latimeria chalumnae... [more]
A0A3P9L7D31.979e-12160.51Glycine N-methyltransferase OS=Oryzias latipes OX=... [more]
A0A3B3H9X61.979e-12160.51Glycine N-methyltransferase OS=Oryzias latipes OX=... [more]
A0A3P9JEI32.900e-12160.51Glycine N-methyltransferase OS=Oryzias latipes OX=... [more]
A0A1A8F5H23.234e-12158.92Glycine N-methyltransferase OS=Nothobranchius kort... [more]
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BLAST of Glycine N-methyltransferase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gnmt1.554e-10555.44glycine N-methyltransferase [Source:ZFIN;Acc:ZDB-G... [more]
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BLAST of Glycine N-methyltransferase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000010933.18.654e-2860.87pep scaffold:Pmarinus_7.0:GL476423:348413:348622:1... [more]
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BLAST of Glycine N-methyltransferase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Glycine N-methyltransferase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO332666.569e-11254.84Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Glycine N-methyltransferase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
gnmt7.450e-12560.51glycine N-methyltransferase [Source:NCBI gene;Acc:... [more]
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BLAST of Glycine N-methyltransferase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000037719.12.752e-16199.57SMESG000037719.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003448 ID=SMED30003448|Name=Glycine N-methyltransferase|organism=Schmidtea mediterranea sexual|type=transcript|length=1103bp
ATAATGTTTTCCATGATTGTGGTGTCAATAAATTCATATAAAATTAGTTT
CAATAAAAACTGACATATGTGGAAGCCAAAAATAAATTAATGACTGCGAC
CCAAGCTGAGAATTCAGTAAGCTCAAAAAACTTTACCAATCAAGACATTA
TGGTTGATAGGATCTATCGAACAAGATCTTTAGGGGTGGCTGTGTCCGGA
TTGCCTGATCAGTATGCTGATGGAAAGGCAGCTAAAGTCTGGGAGTTATA
TTTAGGATCTAAACAAGAAAGAACATCTATTTATAAAGAATGGTTTATAA
ATTTATTGAAGAACCATAATGTAACGAATGTACTTGATGTGGCTTGTGGA
ACTGGGGTCGATTCTGTTATGTTACTTGAAGAAGGCTTTAACGTTATTAG
CACAGATGCAAGTGACAAAATGCTAAAGGTGGCCTTAAAAGAAAGATGGA
ATCGGCGACATGAAAAATATTTTGATCAATGGGTTATTGAGGAAGCGAAC
TGGTTGACGTTGCAACAAGATTTAAATAATCTCAATAACCCAAAGGTTAT
TCCGACAAATGGATTTGATGCAGTGATCTGTCTTGGAAACTCGTTTGCTC
ATCTTCCAGATTTGATAGGCGATCAAAGTGAACAGCAAGTTGCGTTGGCA
AATATGCGTGAATTACTGCGGCCCGATGGAATCCTCGTCATTGACCATCG
AAACTATGATTATATATTAAAAACCGGACAAGCTCCCAAGGGGAAAAATG
TATATTATCCGAGTGACCACATAAAAAAAATTAAAACATCAAACTTGTTT
GTCGATGGAAAACCAACAATGATTACATTGGACTACACGATGGACATCAC
TGGAATAATGACAGAACAACAAATTTTCAAACTGCCTTTACTTTCCGAGG
AAGAACAATACGAATTAAAATTTCAAAACTTTCGGCTATCATACTATCCT
CATACGTTAGACAGGTTTACGGAATTATTACAAAATGTTTTTCAAAACAA
AAAAGAAAAGGTATATGGGGACTTTCAAGATTTAACCAAAATCAAAGATC
CAGCATATTACATTCATGTTATGACGAAATAAATGATTTTGACTTAAAAA
AAA
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protein sequence of SMED30003448-orf-1

>SMED30003448-orf-1 ID=SMED30003448-orf-1|Name=SMED30003448-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=331bp
MTATQAENSVSSKNFTNQDIMVDRIYRTRSLGVAVSGLPDQYADGKAAKV
WELYLGSKQERTSIYKEWFINLLKNHNVTNVLDVACGTGVDSVMLLEEGF
NVISTDASDKMLKVALKERWNRRHEKYFDQWVIEEANWLTLQQDLNNLNN
PKVIPTNGFDAVICLGNSFAHLPDLIGDQSEQQVALANMRELLRPDGILV
IDHRNYDYILKTGQAPKGKNVYYPSDHIKKIKTSNLFVDGKPTMITLDYT
MDITGIMTEQQIFKLPLLSEEEQYELKFQNFRLSYYPHTLDRFTELLQNV
FQNKKEKVYGDFQDLTKIKDPAYYIHVMTK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR029063SAM-dependent_MTases
IPR014369Gly/Sar_N_MeTrfase
Vocabulary: molecular function
TermDefinition
GO:0017174glycine N-methyltransferase activity
GO:0008168methyltransferase activity
GO:0016740transferase activity
Vocabulary: biological process
TermDefinition
GO:0032259methylation