SMED30003432

Overview
NameSMED30003432
Smed IDSMED30003432
Length (bp)1806
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003432 (SMED30003432) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003432 (SMED30003432) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003432

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
reproductive organSMED30003432h1SMcG0006737 Contig7112newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30003432h1SMcG0006737 Contig7112uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30003432h1SMcG0006737 Contig47712newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30003432h1SMcG0006737 Contig47712uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30003432h1SMcG0006737 Contig47712newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30003432h1SMcG0006737 Contig47712uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30003432h1SMcG0006737 Contig47712newmark_estsPMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30003432h1SMcG0006737 Contig47712uc_Smed_v2PMID:28434803
Rouhana et al., 2017
whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003432 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005899:12125..16037 -8846
Homology
BLAST of SMED30003432 vs. Ensembl Human
Match: ABCB1 (ATP binding cassette subfamily B member 1 [Source:HGNC Symbol;Acc:HGNC:40])

HSP 1 Score: 449.899 bits (1156), Expect = 8.751e-144
Identity = 229/565 (40.53%), Postives = 354/565 (62.65%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLT------------------MTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS + +FR++  +D L ++V  +++I  GA  PL +L+F ++ ++  +  +L                   M   E M      ++ + +   ++ +IQ+    L A RQ  ++R  +F+A++RQEI WFD    G+L T+LTDDV  I  GI DK+  F Q +++F    I+ FT GWKL+ +     P++  + ++    +  ++ K++ AY+ AG +AEE+L++IRTV+AF  Q+KE++RY+ NL+EA+ +G+K++I   +  G     I+   +L ++ G   +  G Y  G V+ +F +++IG+ ++G A P +E F  A+ AAY +F++ID  P I S+ K G  P++  G++EF+NV F YP+R  V IL+  N  +  G+TVA +G SG GKST + L+QR YDP  G +S  G D++ I++ + R  IGVV QEP LF+ +I ENI+ G+ +   +EI K+ +++NAY+FIMK   KF+T +G+RGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:   34 VSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDK--ARKGRTTIVIAHRLSTVRNAD 596          

HSP 2 Score: 332.028 bits (850), Expect = 6.902e-100
Identity = 181/497 (36.42%), Postives = 286/497 (57.55%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGT------------VIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F  L   +FI+ F+Q  T        ++RLR   F ++LRQ++ WFD  K  TG L T+L +D   ++  I  +LA   Q I++    II++F  GW+L+ +    +PI+A    + +  +   ++K  K    +G IA E + + RTVV+ + ++K    Y+ +L+      ++++        + G T     +++Y     F Y G +  G             V+ +F  +V G++A+G       ++  AK +A  +  +I++ P I S+   G++P +  G++ F  V F YPTRP +P+LQ  +  +  G+T+A +G SG GKST++ LL+RFYDPLAG++   G ++K +++ W R+H+G+V QEP LF  SI ENI  G    +   EEIV++ +++N + FI     K++T +GD+G   SGGQKQRIAIA ALVR P +LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  759 FLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKA-------HIFGITFSFTQAMMY-----FSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDK--AREGRTCIVIAHRLSTIQNAD 1241          
BLAST of SMED30003432 vs. Ensembl Human
Match: ABCB1 (ATP binding cassette subfamily B member 1 [Source:HGNC Symbol;Acc:HGNC:40])

HSP 1 Score: 449.899 bits (1156), Expect = 8.751e-144
Identity = 229/565 (40.53%), Postives = 354/565 (62.65%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLT------------------MTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS + +FR++  +D L ++V  +++I  GA  PL +L+F ++ ++  +  +L                   M   E M      ++ + +   ++ +IQ+    L A RQ  ++R  +F+A++RQEI WFD    G+L T+LTDDV  I  GI DK+  F Q +++F    I+ FT GWKL+ +     P++  + ++    +  ++ K++ AY+ AG +AEE+L++IRTV+AF  Q+KE++RY+ NL+EA+ +G+K++I   +  G     I+   +L ++ G   +  G Y  G V+ +F +++IG+ ++G A P +E F  A+ AAY +F++ID  P I S+ K G  P++  G++EF+NV F YP+R  V IL+  N  +  G+TVA +G SG GKST + L+QR YDP  G +S  G D++ I++ + R  IGVV QEP LF+ +I ENI+ G+ +   +EI K+ +++NAY+FIMK   KF+T +G+RGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:   34 VSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDK--ARKGRTTIVIAHRLSTVRNAD 596          

HSP 2 Score: 332.028 bits (850), Expect = 6.902e-100
Identity = 181/497 (36.42%), Postives = 286/497 (57.55%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGT------------VIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F  L   +FI+ F+Q  T        ++RLR   F ++LRQ++ WFD  K  TG L T+L +D   ++  I  +LA   Q I++    II++F  GW+L+ +    +PI+A    + +  +   ++K  K    +G IA E + + RTVV+ + ++K    Y+ +L+      ++++        + G T     +++Y     F Y G +  G             V+ +F  +V G++A+G       ++  AK +A  +  +I++ P I S+   G++P +  G++ F  V F YPTRP +P+LQ  +  +  G+T+A +G SG GKST++ LL+RFYDPLAG++   G ++K +++ W R+H+G+V QEP LF  SI ENI  G    +   EEIV++ +++N + FI     K++T +GD+G   SGGQKQRIAIA ALVR P +LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  759 FLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKA-------HIFGITFSFTQAMMY-----FSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDK--AREGRTCIVIAHRLSTIQNAD 1241          
BLAST of SMED30003432 vs. Ensembl Human
Match: ABCB5 (ATP binding cassette subfamily B member 5 [Source:HGNC Symbol;Acc:HGNC:46])

HSP 1 Score: 439.113 bits (1128), Expect = 7.333e-140
Identity = 234/562 (41.64%), Postives = 354/562 (62.99%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSP----------RDLTMT---FSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGS--YDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISH-KKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V   ++FRFA G+D+ ++I+ I++S+  GA  PL  L+  ++ + L+S           ++ T +    +E M    + +  +     I G+IQ+    +TAARQ++R+R  +F+++L Q+I WFD    G+L T++TDD++ I  GI DK+A   Q +S+F   + +    GWKL+ +   + P++  + +     +   + K++ AYS AG +AEE+LSSIRTV+AF AQEKE++RY+ NLK+A+  G+KR+I   +  G + F +     L ++ G   I  G   Y  GTV+ +F +++  S  +G AVP  E F  A+ AA+ +FQVID+ P I +    G  PES  G +EFKNV F YP+RP + IL+  N  +  G+TVA +G +GSGKST++ LLQR YDP  G I     D++ +++  YR HIGVV QEP LF  +I  NI+ G+ D  +EE+ ++ +++NAY+FIM+   KFNT +G++GA  SGGQKQRIAIA ALVRNP++L+LDEATSALD+ S+  VQ  L+K  A   RT ++VAHRL T+R+AD
Sbjct:   31 VGSIEIFRFADGLDITLMILGILASLVNGACLPLMPLVLGEMSDNLISGCLVQTNTTNYQNCTQSQEKLNEDMTLLTLYYVGIGVAALIFGYIQISLWIITAARQTKRIRKQFFHSVLAQDIGWFDSCDIGELNTRMTDDIDKISDGIGDKIALLFQNMSTFSIGLAVGLVKGWKLTLVTLSTSPLIMASAAACSRMVISLTSKELSAYSKAGAVAEEVLSSIRTVIAFRAQEKELQRYTQNLKDAKDFGIKRTIASKVSLGAVYFFMNGTYGLAFWYGTSLILNGEPGYTIGTVLAVFFSVIHSSYCIGAAVPHFETFAIARGAAFHIFQVIDKKPSIDNFSTAGYKPESIEGTVEFKNVSFNYPSRPSIKILKGLNLRIKSGETVALVGLNGSGKSTVVQLLQRLYDPDDGFIMVDENDIRALNVRHYRDHIGVVSQEPVLFGTTISNNIKYGRDDVTDEEMERAAREANAYDFIMEFPNKFNTLVGEKGAQMSGGQKQRIAIARALVRNPKILILDEATSALDSESKSAVQAALEK--ASKGRTTIVVAHRLSTIRSAD 590          

HSP 2 Score: 310.457 bits (794), Expect = 3.081e-92
Identity = 185/531 (34.84%), Postives = 289/531 (54.43%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQ--TGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNL--------KEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAIN--EEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G   P+  +IF  ++ +  +    T+     +   +  F IL    F+S F+Q +         + RLR   F A+L Q+I WFD+++  TG L T L  D+  I+     ++    Q  ++    +I++F  GW+++F+     P++A T  +    +  ++ K  +    AG IA E L +IRT+V+ + ++   + Y   L        K+A+ +G     C       I F  +  G   +  G   I  G   P  +  +F  I  G++A+G  + +   +  AK+ A  LF ++++ P I S  + G  P++  G++EF+ V F YP RP V IL+  + ++  GKTVAF+G SG GKST + LLQR YDP+ G++ F G D K++++ W RS I +V QEP LF+ SI ENI  G    +   +EI ++   +N + FI    +K+NT +G +GA  SGGQKQR+AIA AL++ P++LLLDEATSALDN SEK+VQ  LDK  A   RT ++V HRL  ++NAD
Sbjct:  703 GTVHPVFSIIFAKIITMFGNNDKTTLKHDAEIYSMI--FVILGVICFVSYFMQGLFYGRAGEILTMRLRHLAFKAMLYQDIAWFDEKENSTGGLTTILAIDIAQIQGATGSRIGVLTQNATNMGLSVIISFIYGWEMTFLILSIAPVLAVTGMIETAAMTGFANKDKQELKHAGKIATEALENIRTIVSLTREKAFEQMYEEMLQTQHRNTSKKAQIIGS----CYAFSHAFIYFA-YAAG---FRFGAYLIQAGRMTPEGMFIVFTAIAYGAMAIGETLVLAPEYSKAKSGAAHLFALLEKKPNIDSRSQEGKKPDTCEGNLEFREVSFFYPCRPDVFILRGLSLSIERGKTVAFVGSSGCGKSTSVQLLQRLYDPVQGQVLFDGVDAKELNVQWLRSQIAIVPQEPVLFNCSIAENIAYGDNSRVVPLDEIKEAANAANIHSFIEGLPEKYNTQVGLKGAQLSGGQKQRLAIARALLQKPKILLLDEATSALDNDSEKVVQHALDK--ARTGRTCLVVTHRLSAIQNAD 1221          
BLAST of SMED30003432 vs. Ensembl Human
Match: ABCB4 (ATP binding cassette subfamily B member 4 [Source:HGNC Symbol;Acc:HGNC:45])

HSP 1 Score: 419.083 bits (1076), Expect = 1.769e-132
Identity = 226/556 (40.65%), Postives = 341/556 (61.33%), Query Frame = -1
Query:    1 LFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVS-------PRDLTMTF-------SERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1623
            LFR++   D L + +  I +I  G+  PL +++F ++ +  V        P + +++         E M      ++ L +   ++ +IQ+   +L A RQ R++R  +F+A+LRQEI WFD   T +L T+LTDD+  I  GI DK+  F Q +++F    I+ F  GWKL+ +     PI+  + ++    +  +S K++ AY+ AG +AEE L +IRTV+AF  Q KE++RY  +L+ A+ +G+K++I   +  G+    I+   +L ++ G   +    Y  G  + +F +I+IG+ ++G A P ++ F  A+ AAY +F +ID  P+I S  + G  P+S  G++EF +V F YP+R  V IL+  N  +  G+TVA +G SG GKST + L+QR YDP  G I+  G D+++ ++ + R  IGVV QEP LFS +I ENI  G+ +   +EI K+ +++NAYEFIMK  QKF+T +G+RGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE  VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:   45 LFRYSDWQDKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDKFVDTAGNFSFPVNFSLSLLNPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGNVTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDK--AREGRTTIVIAHRLSTVRNAD 598          

HSP 2 Score: 317.39 bits (812), Expect = 7.861e-95
Identity = 182/485 (37.53%), Postives = 263/485 (54.23%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F  L   +F + F+Q  T        +RRLRS  F A+LRQ++ WFD  K  TG L T+L  D   ++     +LA   Q I++    II++F  GW+L+ +    +PI+A +  + +  +   + +  K    AG IA E + +IRTVV+ + + K    Y   L                                            Y P    ++F  IV G++ALG+A     ++  AK +A  LF + +R P I S+ + G+ P+ F G+I F  V F YPTR  VP+LQ  +  +  G+T+A +G SG GKST++ LL+RFYDPLAG +   G + K +++ W R+ +G+V QEP LF  SI ENI  G    +   +EIV + + +N + FI     K+ T +GD+G   SGGQKQRIAIA AL+R PQ+LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  758 FLFLGIISFFTFFLQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQERKFESMYVEKL--------------------------------------------YGP---YRVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDK--AREGRTCIVIAHRLSTIQNAD 1193          
BLAST of SMED30003432 vs. Ensembl Human
Match: ABCB4 (ATP binding cassette subfamily B member 4 [Source:HGNC Symbol;Acc:HGNC:45])

HSP 1 Score: 419.083 bits (1076), Expect = 1.769e-132
Identity = 226/556 (40.65%), Postives = 341/556 (61.33%), Query Frame = -1
Query:    1 LFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVS-------PRDLTMTF-------SERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1623
            LFR++   D L + +  I +I  G+  PL +++F ++ +  V        P + +++         E M      ++ L +   ++ +IQ+   +L A RQ R++R  +F+A+LRQEI WFD   T +L T+LTDD+  I  GI DK+  F Q +++F    I+ F  GWKL+ +     PI+  + ++    +  +S K++ AY+ AG +AEE L +IRTV+AF  Q KE++RY  +L+ A+ +G+K++I   +  G+    I+   +L ++ G   +    Y  G  + +F +I+IG+ ++G A P ++ F  A+ AAY +F +ID  P+I S  + G  P+S  G++EF +V F YP+R  V IL+  N  +  G+TVA +G SG GKST + L+QR YDP  G I+  G D+++ ++ + R  IGVV QEP LFS +I ENI  G+ +   +EI K+ +++NAYEFIMK  QKF+T +G+RGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE  VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:   45 LFRYSDWQDKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDKFVDTAGNFSFPVNFSLSLLNPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTLAAGRQIRKIRQKFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVATFFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHFSYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEGTINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGNVTMDEIKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDK--AREGRTTIVIAHRLSTVRNAD 598          

HSP 2 Score: 317.39 bits (812), Expect = 7.861e-95
Identity = 182/485 (37.53%), Postives = 263/485 (54.23%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F  L   +F + F+Q  T        +RRLRS  F A+LRQ++ WFD  K  TG L T+L  D   ++     +LA   Q I++    II++F  GW+L+ +    +PI+A +  + +  +   + +  K    AG IA E + +IRTVV+ + + K    Y   L                                            Y P    ++F  IV G++ALG+A     ++  AK +A  LF + +R P I S+ + G+ P+ F G+I F  V F YPTR  VP+LQ  +  +  G+T+A +G SG GKST++ LL+RFYDPLAG +   G + K +++ W R+ +G+V QEP LF  SI ENI  G    +   +EIV + + +N + FI     K+ T +GD+G   SGGQKQRIAIA AL+R PQ+LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  758 FLFLGIISFFTFFLQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLLAVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQERKFESMYVEKL--------------------------------------------YGP---YRVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLFERQPLIDSYSEEGLKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGTVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLPHKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDEATSALDTESEKVVQEALDK--AREGRTCIVIAHRLSTIQNAD 1193          
BLAST of SMED30003432 vs. Ensembl Celegans
Match: pgp-9 (p-GlycoProtein related [Source:UniProtKB/TrEMBL;Acc:G5EG58])

HSP 1 Score: 402.519 bits (1033), Expect = 2.844e-126
Identity = 222/561 (39.57%), Postives = 326/561 (58.11%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVN-------LLVSPRDLT-------MTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            +S +QL+R+ + +D L++ V II S   G   PL  +I  +V         + + P             FS  +  + +K+  L    F +GF+Q     +   + S R R  +F++++RQEI W+DK  +G L  KL D++E +  G  DK+    Q ++ F+G   +AFT  W L+ I     P M      +   +   + K+ K Y++AGGIAEE+L+SIRTV+AF+ QE E KRY   L+  +  G+K+S   G G       I+    L +++G  F+Y G  + GTV+ +F ++++GS+ALG A        TA  AA  L++VIDR+P+I ++   G  P    G I    V+F YPTR  V IL+  +     G+TVA +G SG GKSTII LLQRFY+P AG+I      ++D ++ + R  +GVV QEP LF+ SI++NI+ G+ D  +E+I ++ +++NA +FI    +  NT +GDRG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  L+   A   RT +++AHRL TVRNAD
Sbjct:   29 ISIFQLYRYTSTVDRLMLAVGIIVSCATGVGLPLMSIIMGNVSQNFVTLGTIFLDPNSTASEKAAARAEFSHEVIQNCLKYVYLGCGIFAAGFLQASCFMVICEKLSNRFRRQFFHSVMRQEIAWYDKNTSGTLSNKLFDNLERVREGTGDKVGLAFQMMAQFIGGFAVAFTYDWLLTLIMMSLSPFMMICGLFLAKLLATAATKEAKQYAVAGGIAEEVLTSIRTVIAFNGQEYECKRYEDALEHGKKTGIKKSFLIGAGLASFFVIIYASYCLAFWVGTNFVYSGRLESGTVLTVFFSVMMGSMALGQAGQQFATIGTALGAAASLYEVIDRIPEIDAYSTEGQTPSKISGRISVNKVEFTYPTRADVKILKGVSLDAQPGQTVALVGSSGCGKSTIIQLLQRFYNPDAGQILIDDIPIEDFNIKYLRQLVGVVSQEPNLFNTSIEQNIRYGRSDVSDEDIARALKEANAADFIKTFPEGLNTLVGDRGVQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESESIVQSALEN--ASRGRTTIVIAHRLSTVRNAD 587          

HSP 2 Score: 298.516 bits (763), Expect = 3.303e-88
Identity = 182/524 (34.73%), Postives = 275/524 (52.48%), Query Frame = -1
Query:    4 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGS--YDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKM--DAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNA 1554
            GA  P   L F  ++N+  +P    M          + F +L++    S   Q     + A R + R+RS  +  +LRQ+  +FD  K   G++ T+L  D  +I+S I  +L +    I+S  G + +AF  GW+++F+     P MA   +LM+ +    +    K    AG  A E + +IRTV A + Q K    +  +L       + ++I +GL  G      F   +  +  GL  I++ +   +P  V+++   I      +G A      +I A  AA  +F +++  P+I      G  P+   G+++   V F YP RP VPILQ  N  +  G+T+A +GPSG GKST+I LL+R YDPL G ++    DL+ ++    R HI +V QEP LF  SI+ENI  G    +  +E+I  +   +N ++FI +    + T +G++G   SGGQKQRIAIA AL+RNP++LLLDEATSALD  SEK VQ  LD   A   RT ++VAHRL T+ NA
Sbjct:  717 GAVMPAFSLFFSQIINVFSNPDRDQMKKDGHFW--ALMFLVLAAVQGTSMLFQCSLFGVAAERLTMRIRSKVYRNVLRQDATYFDMPKHSPGRITTRLATDAPNIKSAIDYRLGSIFNAIASVGGGLGIAFYYGWQMAFLVMAIFPFMAVGQALMMKYHGGSATSDAKEMENAGKTAMEAIENIRTVQALTLQTKLYNIFCSHLDAPHGGNISKAIIRGLTYGFANSIQFFTYAAAFRFGLFLIFDKNVLMEPENVLRVLFAISFSFGTIGFAASYFPEYIKATFAAGLIFNMLEEEPRIDGMTSSGTYPQ-LSGEVKLNKVFFRYPERPAVPILQGLNVHVKPGQTLALVGPSGCGKSTVISLLERLYDPLEGAVTVDNNDLRQMNPKHLRKHIALVSQEPILFDTSIRENIVYGLQPGEYTHEQIETACSKANIHKFIDELPDGYETRVGEKGTQLSGGQKQRIAIARALIRNPKILLLDEATSALDTESEKQVQVALDA--AAKDRTCIVVAHRLSTIVNA 1235          
BLAST of SMED30003432 vs. Ensembl Celegans
Match: pgp-1 (Multidrug resistance protein pgp-1 [Source:UniProtKB/Swiss-Prot;Acc:P34712])

HSP 1 Score: 389.808 bits (1000), Expect = 2.424e-121
Identity = 221/563 (39.25%), Postives = 336/563 (59.68%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPL---------------NVLIFRDVVNLLVSPRDLTMT-FSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS  QL+R+ T ++ L++ +  + ++  GA  PL                ++I  +    L + ++ T T F   +   V  +A ++   + +G I +      A + + RLR  +  ++LRQEI WFD   +G L TKL D++E ++ G  DK+    Q +S F+   I+AFT  W+L+ +     PI A     +   +  ++I++   Y+ AG + EE +SSIRTVV+ +    E++RYS  ++EA+  GV + +  G+  G +  + F   +L +Y+G+ ++++GS + G ++  F ++++GS+ALG A P L    TA+ AA  +++V+DR P I S  K G       GDI  +NV F YP+RP VPIL+  N  +  G+TVA +G SG GKSTII LL R+YD L G+I+  G D++DI+L + R ++ VV QEP LF+ +I+ENI LGK     EE+V + + +NA +FI      +NT +GDRG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ+ LDK  A   RT +++AHRL T+RNAD
Sbjct:   60 VSIPQLYRYTTTLEKLLLFIGTLVAVITGAGLPLMSILQGKVSQAFINEQIVINNNGSTFLPTGQNYTKTDFEHDVMNVVWSYAAMTVGMWAAGQITVTCYLYVAEQMNNRLRREFVKSILRQEISWFDTNHSGTLATKLFDNLERVKEGTGDKIGMAFQYLSQFITGFIVAFTHSWQLTLVMLAVTPIQALCGFAIAKSMSTFAIRETLRYAKAGKVVEETISSIRTVVSLNGLRYELERYSTAVEEAKKAGVLKGLFLGISFGAMQASNFISFALAFYIGVGWVHDGSLNFGDMLTTFSSVMMGSMALGLAGPQLAVLGTAQGAASGIYEVLDRKPVIDSSSKAGRKDMKIKGDITVENVHFTYPSRPDVPILRGMNLRVNAGQTVALVGSSGCGKSTIISLLLRYYDVLKGKITIDGVDVRDINLEFLRKNVAVVSQEPALFNCTIEENISLGKEGITREEMVAACKMANAEKFIKTLPNGYNTLVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDAESEGIVQQALDK--AAKGRTTIIIAHRLSTIRNAD 620          

HSP 2 Score: 293.508 bits (750), Expect = 2.010e-86
Identity = 172/488 (35.25%), Postives = 268/488 (54.92%), Query Frame = -1
Query:    1 IKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQ--TGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEG--SYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1446
            + F +L++   I  F+    + + +   +R LR+  F  +L Q I +FD  Q  +G++ T+L  DV ++ + I  + +  I  + S V  I LAF  GW+++ +    +PI+A    L        ++K    ++ +G IA E + ++RTV A + ++   + +   L       +K +  QGL  G     ++ + +  Y MGL  I     +  P  V+++   I I +  LG A      +  A  A   +F ++ ++ +I         +  YG + FKNV F YP RP + IL+  +F++  G+T+A +GPSG GKST++ LL+RFYD L GEI   G+++K ++    RS I +V QEP LF  SI ENI  G         ++ ++ + +N + FI +  + F T +GDRG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SEK+VQ+ LD+  A   RT +++AHRL TV NAD
Sbjct:  798 LMFLVLAAAQGICSFLMTFFMGIASESLTRDLRNKLFRNVLSQHIGFFDSPQNASGKISTRLATDVPNLRTAIDFRFSTVITTLVSMVAGIGLAFFYGWQMALLIIAILPIVAFGQYLRGRRFTGKNVKSASEFADSGKIAIEAIENVRTVQALAREDTFYENFCEKLDIPHKEAIKEAFIQGLSYGCASSVLYLLNTCAYRMGLALIITDPPTMQPMRVLRVMYAITISTSTLGFATSYFPEYAKATFAGGIIFGMLRKISKIDSLSLAGEKKKLYGKVIFKNVRFAYPERPEIEILKGLSFSVEPGQTLALVGPSGCGKSTVVALLERFYDTLGGEIFIDGSEIKTLNPEHTRSQIAIVSQEPTLFDCSIAENIIYGLDPSSVTMAQVEEAARLANIHNFIAELPEGFETRVGDRGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEKVVQEALDR--AREGRTCIVIAHRLNTVMNAD 1283          
BLAST of SMED30003432 vs. Ensembl Celegans
Match: pgp-2 (ABC transporter PGP-2; p-GlycoProtein related [Source:UniProtKB/TrEMBL;Acc:G5EDF9])

HSP 1 Score: 377.096 bits (967), Expect = 4.938e-117
Identity = 202/563 (35.88%), Postives = 329/563 (58.44%), Query Frame = -1
Query:    1 YQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLL--------------VSPRDLTMTFSERMRPSVIKFAI----LSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMG-LLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1629
            Y +F +  G+DLL++I   ++++  GA FPL  ++   +  +               V+P  L     +     V+K+ I    L    F + ++Q+      A R   +LR NY  A+LRQ+I WFDKQQTG L  +LTDD+E +  G+ DK A  +Q  ++F+    + F   W ++ +     P++  + + M   +   +  + + Y++AG IAEE  SSIRTV + +  ++E+ R+ + L+     G+ +    G+G G     +++  +L ++ G  L I + ++D G +  +F  ++ GS +LG A+P L +F TA+ AA  + +VI+  P+I  +   G++ ++  GDI FK+V F YP+R  + +L+  +  L  G  +A +G SG GKSTI++LLQRFYDP  G +   G DL+++++   R  IG+V QEP LF G+I ENI++G   A ++++V++ + +NA +FI +    + T +G++G   SGGQKQRIAIA ALV+NP++LLLDEATSALD  +E+ VQ  LD+  A+  RT ++VAHRL T+RN D
Sbjct:   36 YGIFYYTQGVDLLLLITGTVAAVIHGAGFPLLAIVLGGMTTVFLRAQNSDFVVGVDNVNPEGLVPISLDEFNSEVVKYCIYYLVLGVLMFFTSYVQIACFESYAERLVHKLRQNYLKAILRQQIQWFDKQQTGNLTARLTDDLERVREGLGDKFALLVQMFAAFLAGYGVGFFYSWSMTLVMMGFAPLIVLSGAKMSKSMATRTRVEQETYAVAGAIAEETFSSIRTVHSLNGHKRELDRFYNALEVGRQTGIVKYCYMGIGVGFSNLCMYSSYALAFWYGSTLIINDPTFDRGLIFTVFFAVLSGSTSLGGALPHLASFGTARGAASTVLRVINSHPKIDPYSLEGILVDNMKGDISFKDVHFRYPSRKDIHVLKGISLELKAGDKIALVGSSGCGKSTIVNLLQRFYDPTKGRVLIDGVDLREVNVHSLREQIGIVSQEPVLFDGTIYENIKMGNEHATHDQVVEACKMANANDFIKRLPDGYGTRVGEKGVQLSGGQKQRIAIARALVKNPKILLLDEATSALDTEAEREVQGALDQ--AQAGRTTIIVAHRLSTIRNVD 596          

HSP 2 Score: 276.944 bits (707), Expect = 1.113e-80
Identity = 177/535 (33.08%), Postives = 286/535 (53.46%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFA---ILSSCTFISGFIQMVT-ISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIP--IMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEK------EVKR--YSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG-KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G+  P+  L++ ++ N+   P D       +M+ +V  +    +L   TF  GF      +       + +LR   F  LLRQ+I ++D  +  TG+L T+   D  ++   +  +L   +  I +  G + + F  GW+L+ I  + +P  +M   F + + F K+  I+  +    AG +A + +  IRTV + + QE+      E  R  ++ NLK A T G   +  Q L        IF + +  +Y+G +F+ + +  P  V ++F  I      +GN    + + + A+ AA  LF +I+    I       I +   G+I  +NV F YPTR    +LQ F   +  GKTVA +G SG GKSTI+ LL+RFY+   G I   G ++++++++  R  + +V QEP LF  +I ENI  G   +   +EIV++ + +N + FI+     ++T +G++G   SGGQKQRIAIA ALVR+P +LLLDEATSALD  SEK+VQ+ LD   A+  RT +++AHRL T++N+D
Sbjct:  712 GSVTPVFALVYAEIFNVYSLPAD-------QMQANVYFWCGMFVLMGITFFVGFFTSANCLGRCGESLTMKLRFEAFKNLLRQDIAFYDDLRHGTGKLCTRFATDAPNVRY-VFTRLPVVLASIVTICGALGIGFYYGWQLALILVVMVPLLVMGGYFEMQMRFGKQ--IRDTQLLEEAGKVASQAVEHIRTVHSLNRQEQFHFTYCEYLREPFNTNLKHAHTYGAVFAFSQSL--------IFFMYAAAFYLGSIFVNQQAMQPIDVYRVFFAISFCGQMIGNTTSFIPDVVKARLAASLLFYLIEHPTPIDSLSDSGIVKPITGNISIRNVFFNYPTRKDTKVLQGFTLDIKAGKTVALVGHSGCGKSTIMGLLERFYNQDKGMIMIDGDNIRNLNISSLREQVCIVSQEPTLFDCTIGENICYGTNRNVTYQEIVEAAKMANIHNFILGLPDGYDTHVGEKGTQLSGGQKQRIAIARALVRSPSVLLLDEATSALDTESEKIVQEALDA--AKQGRTCLVIAHRLSTIQNSD 1226          
BLAST of SMED30003432 vs. Ensembl Celegans
Match: pgp-2 (ABC transporter PGP-2; p-GlycoProtein related [Source:UniProtKB/TrEMBL;Acc:G5EDF9])

HSP 1 Score: 377.096 bits (967), Expect = 5.628e-117
Identity = 202/563 (35.88%), Postives = 329/563 (58.44%), Query Frame = -1
Query:    1 YQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLL--------------VSPRDLTMTFSERMRPSVIKFAI----LSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMG-LLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1629
            Y +F +  G+DLL++I   ++++  GA FPL  ++   +  +               V+P  L     +     V+K+ I    L    F + ++Q+      A R   +LR NY  A+LRQ+I WFDKQQTG L  +LTDD+E +  G+ DK A  +Q  ++F+    + F   W ++ +     P++  + + M   +   +  + + Y++AG IAEE  SSIRTV + +  ++E+ R+ + L+     G+ +    G+G G     +++  +L ++ G  L I + ++D G +  +F  ++ GS +LG A+P L +F TA+ AA  + +VI+  P+I  +   G++ ++  GDI FK+V F YP+R  + +L+  +  L  G  +A +G SG GKSTI++LLQRFYDP  G +   G DL+++++   R  IG+V QEP LF G+I ENI++G   A ++++V++ + +NA +FI +    + T +G++G   SGGQKQRIAIA ALV+NP++LLLDEATSALD  +E+ VQ  LD+  A+  RT ++VAHRL T+RN D
Sbjct:   43 YGIFYYTQGVDLLLLITGTVAAVIHGAGFPLLAIVLGGMTTVFLRAQNSDFVVGVDNVNPEGLVPISLDEFNSEVVKYCIYYLVLGVLMFFTSYVQIACFESYAERLVHKLRQNYLKAILRQQIQWFDKQQTGNLTARLTDDLERVREGLGDKFALLVQMFAAFLAGYGVGFFYSWSMTLVMMGFAPLIVLSGAKMSKSMATRTRVEQETYAVAGAIAEETFSSIRTVHSLNGHKRELDRFYNALEVGRQTGIVKYCYMGIGVGFSNLCMYSSYALAFWYGSTLIINDPTFDRGLIFTVFFAVLSGSTSLGGALPHLASFGTARGAASTVLRVINSHPKIDPYSLEGILVDNMKGDISFKDVHFRYPSRKDIHVLKGISLELKAGDKIALVGSSGCGKSTIVNLLQRFYDPTKGRVLIDGVDLREVNVHSLREQIGIVSQEPVLFDGTIYENIKMGNEHATHDQVVEACKMANANDFIKRLPDGYGTRVGEKGVQLSGGQKQRIAIARALVKNPKILLLDEATSALDTEAEREVQGALDQ--AQAGRTTIIVAHRLSTIRNVD 603          

HSP 2 Score: 276.944 bits (707), Expect = 1.155e-80
Identity = 177/535 (33.08%), Postives = 286/535 (53.46%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFA---ILSSCTFISGFIQMVT-ISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIP--IMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEK------EVKR--YSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG-KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G+  P+  L++ ++ N+   P D       +M+ +V  +    +L   TF  GF      +       + +LR   F  LLRQ+I ++D  +  TG+L T+   D  ++   +  +L   +  I +  G + + F  GW+L+ I  + +P  +M   F + + F K+  I+  +    AG +A + +  IRTV + + QE+      E  R  ++ NLK A T G   +  Q L        IF + +  +Y+G +F+ + +  P  V ++F  I      +GN    + + + A+ AA  LF +I+    I       I +   G+I  +NV F YPTR    +LQ F   +  GKTVA +G SG GKSTI+ LL+RFY+   G I   G ++++++++  R  + +V QEP LF  +I ENI  G   +   +EIV++ + +N + FI+     ++T +G++G   SGGQKQRIAIA ALVR+P +LLLDEATSALD  SEK+VQ+ LD   A+  RT +++AHRL T++N+D
Sbjct:  719 GSVTPVFALVYAEIFNVYSLPAD-------QMQANVYFWCGMFVLMGITFFVGFFTSANCLGRCGESLTMKLRFEAFKNLLRQDIAFYDDLRHGTGKLCTRFATDAPNVRY-VFTRLPVVLASIVTICGALGIGFYYGWQLALILVVMVPLLVMGGYFEMQMRFGKQ--IRDTQLLEEAGKVASQAVEHIRTVHSLNRQEQFHFTYCEYLREPFNTNLKHAHTYGAVFAFSQSL--------IFFMYAAAFYLGSIFVNQQAMQPIDVYRVFFAISFCGQMIGNTTSFIPDVVKARLAASLLFYLIEHPTPIDSLSDSGIVKPITGNISIRNVFFNYPTRKDTKVLQGFTLDIKAGKTVALVGHSGCGKSTIMGLLERFYNQDKGMIMIDGDNIRNLNISSLREQVCIVSQEPTLFDCTIGENICYGTNRNVTYQEIVEAAKMANIHNFILGLPDGYDTHVGEKGTQLSGGQKQRIAIARALVRSPSVLLLDEATSALDTESEKIVQEALDA--AKQGRTCLVIAHRLSTIQNSD 1233          
BLAST of SMED30003432 vs. Ensembl Celegans
Match: pgp-2 (ABC transporter PGP-2; p-GlycoProtein related [Source:UniProtKB/TrEMBL;Acc:G5EDF9])

HSP 1 Score: 377.481 bits (968), Expect = 6.183e-117
Identity = 202/563 (35.88%), Postives = 329/563 (58.44%), Query Frame = -1
Query:    1 YQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLL--------------VSPRDLTMTFSERMRPSVIKFAI----LSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMG-LLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1629
            Y +F +  G+DLL++I   ++++  GA FPL  ++   +  +               V+P  L     +     V+K+ I    L    F + ++Q+      A R   +LR NY  A+LRQ+I WFDKQQTG L  +LTDD+E +  G+ DK A  +Q  ++F+    + F   W ++ +     P++  + + M   +   +  + + Y++AG IAEE  SSIRTV + +  ++E+ R+ + L+     G+ +    G+G G     +++  +L ++ G  L I + ++D G +  +F  ++ GS +LG A+P L +F TA+ AA  + +VI+  P+I  +   G++ ++  GDI FK+V F YP+R  + +L+  +  L  G  +A +G SG GKSTI++LLQRFYDP  G +   G DL+++++   R  IG+V QEP LF G+I ENI++G   A ++++V++ + +NA +FI +    + T +G++G   SGGQKQRIAIA ALV+NP++LLLDEATSALD  +E+ VQ  LD+  A+  RT ++VAHRL T+RN D
Sbjct:   36 YGIFYYTQGVDLLLLITGTVAAVIHGAGFPLLAIVLGGMTTVFLRAQNSDFVVGVDNVNPEGLVPISLDEFNSEVVKYCIYYLVLGVLMFFTSYVQIACFESYAERLVHKLRQNYLKAILRQQIQWFDKQQTGNLTARLTDDLERVREGLGDKFALLVQMFAAFLAGYGVGFFYSWSMTLVMMGFAPLIVLSGAKMSKSMATRTRVEQETYAVAGAIAEETFSSIRTVHSLNGHKRELDRFYNALEVGRQTGIVKYCYMGIGVGFSNLCMYSSYALAFWYGSTLIINDPTFDRGLIFTVFFAVLSGSTSLGGALPHLASFGTARGAASTVLRVINSHPKIDPYSLEGILVDNMKGDISFKDVHFRYPSRKDIHVLKGISLELKAGDKIALVGSSGCGKSTIVNLLQRFYDPTKGRVLIDGVDLREVNVHSLREQIGIVSQEPVLFDGTIYENIKMGNEHATHDQVVEACKMANANDFIKRLPDGYGTRVGEKGVQLSGGQKQRIAIARALVKNPKILLLDEATSALDTEAEREVQGALDQ--AQAGRTTIIVAHRLSTIRNVD 596          

HSP 2 Score: 276.944 bits (707), Expect = 1.247e-80
Identity = 177/535 (33.08%), Postives = 286/535 (53.46%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFA---ILSSCTFISGFIQMVT-ISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIP--IMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEK------EVKR--YSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG-KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G+  P+  L++ ++ N+   P D       +M+ +V  +    +L   TF  GF      +       + +LR   F  LLRQ+I ++D  +  TG+L T+   D  ++   +  +L   +  I +  G + + F  GW+L+ I  + +P  +M   F + + F K+  I+  +    AG +A + +  IRTV + + QE+      E  R  ++ NLK A T G   +  Q L        IF + +  +Y+G +F+ + +  P  V ++F  I      +GN    + + + A+ AA  LF +I+    I       I +   G+I  +NV F YPTR    +LQ F   +  GKTVA +G SG GKSTI+ LL+RFY+   G I   G ++++++++  R  + +V QEP LF  +I ENI  G   +   +EIV++ + +N + FI+     ++T +G++G   SGGQKQRIAIA ALVR+P +LLLDEATSALD  SEK+VQ+ LD   A+  RT +++AHRL T++N+D
Sbjct:  736 GSVTPVFALVYAEIFNVYSLPAD-------QMQANVYFWCGMFVLMGITFFVGFFTSANCLGRCGESLTMKLRFEAFKNLLRQDIAFYDDLRHGTGKLCTRFATDAPNVRY-VFTRLPVVLASIVTICGALGIGFYYGWQLALILVVMVPLLVMGGYFEMQMRFGKQ--IRDTQLLEEAGKVASQAVEHIRTVHSLNRQEQFHFTYCEYLREPFNTNLKHAHTYGAVFAFSQSL--------IFFMYAAAFYLGSIFVNQQAMQPIDVYRVFFAISFCGQMIGNTTSFIPDVVKARLAASLLFYLIEHPTPIDSLSDSGIVKPITGNISIRNVFFNYPTRKDTKVLQGFTLDIKAGKTVALVGHSGCGKSTIMGLLERFYNQDKGMIMIDGDNIRNLNISSLREQVCIVSQEPTLFDCTIGENICYGTNRNVTYQEIVEAAKMANIHNFILGLPDGYDTHVGEKGTQLSGGQKQRIAIARALVRSPSVLLLDEATSALDTESEKIVQEALDA--AKQGRTCLVIAHRLSTIQNSD 1250          
BLAST of SMED30003432 vs. Ensembl Fly
Match: Mdr50 (gene:FBgn0010241 transcript:FBtr0087537)

HSP 1 Score: 401.364 bits (1030), Expect = 1.370e-125
Identity = 226/593 (38.11%), Postives = 355/593 (59.87%), Query Frame = -1
Query:    1 KEKPAKSKTDIXXXXXXXXXXXXXXSVSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVN-------LLVSPRD------LTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEG-------SYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISH-KKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1716
            K+K    ++D  + +        ++ VSY+QLFR+AT  D  + ++ ++S++  G   P N LIF ++ N       LL S +       ++    +++R   ++   +     +  ++ +   +  A  Q   +RS +F ++L Q++ W+D  Q+G++ +++ +D+  +E G+++K+  F+  + +FVG ++LAF  GW+LS +   S+P+      L+     + + K++  Y+ A  +AE  LS IRTV AF  + KEV  Y   +  A+ L +KR++  G+G G++ F I+   +L ++ G+  + +G       +YD GT+I +F ++++GS+ +G A P +E F  AK A   +F +I+++P+I+     G         IEFK V+F+YPTRP V IL   N  +  G+TVA +GPSG GKST I L+QRFYDP AG + F+GT+LKD+D+ W RS IGVV QEP LF+ SI ENI+ G+ DA  EEI  +   +NA  FI K  + ++T +G+RGA  SGGQKQRIAIA AL+R+P++LLLDEATSALD  SE  VQ  L+K  A   RT ++VAHRL TVR AD
Sbjct:   45 KKKSKHDESDASDEEDGSQYHEDVKQVSYFQLFRYATKKDRALYVIGLLSAVATGLTTPANSLIFGNLANDMIDLGGLLESGKSYRADDAISTLLLDKVRQFSLQNTYIGIIMLVCSYLSITCFNYAAHSQILTIRSKFFRSILHQDMKWYDFNQSGEVASRMNEDLSKMEDGLAEKVVMFVHYLVAFVGSLVLAFVKGWQLSLVCLTSLPLTFIAMGLVAVATSRLAKKEVTMYAGAAVVAEGALSGIRTVKAFEGEAKEVAAYKERVVAAKILNIKRNMFSGIGFGLLWFFIYASYALAFWYGVGLVIKGYHEPAYENYDAGTMITVFFSVMMGSMNIGMAAPYIEAFGIAKGACAKVFHIIEQIPEINPIDGEGKKLNEPLTTIEFKEVEFQYPTRPEVSILNKLNLKIHRGQTVALVGPSGCGKSTCIQLVQRFYDPQAGNLLFNGTNLKDLDINWLRSRIGVVGQEPILFATSIYENIRYGREDATREEIEAAAAAANAAIFIKKLPKGYDTLVGERGAQLSGGQKQRIAIARALIRDPEILLLDEATSALDTASEAKVQAALEKVSA--GRTTIIVAHRLSTVRRAD 635          

HSP 2 Score: 283.878 bits (725), Expect = 6.217e-83
Identity = 172/526 (32.70%), Postives = 277/526 (52.66%), Query Frame = -1
Query:    1 VGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQ--TGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVI-GFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFY--GDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGK--MDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1557
            +G A P+  ++F  ++ +L S +D      E      + F I      I+ F+Q+    +   R + RLR   F A+LRQE+ WFD +   TG L  +L+ D  +++     ++   +Q IS+    I L+    W L  +     P +   F +    + K ++   K       +A E++S+IRTV +   +E   + Y   L  A  +  + +  +GL  G+      F   + +YY     I+ G    G V ++   +++G+ ++ NA+    N     +AA  +F  + R P I  +  GV  + ++  G + F  V F YPTR  + +L+     +  G+ +A +GPSG GKST I L+QRFYD   G       D++++ +T  R+ +G+V QEP LF  +I+ENI  G    +  ++EI+ + + SN +EFI      ++T +G++GA  SGGQKQRIAIA AL+RNP+++LLDEATSALD  SEK+VQ  LD   +EG RT + +AHRL TV ++D
Sbjct:  756 MGCAMPIFAVLFGSILQIL-SVKDNDQYVRENSNQYSLYFLIAGIVVGIATFLQIYFFGIAGERLTERLRGLMFEAMLRQEVAWFDDKANGTGSLCARLSGDAAAVQGATGQRIGTIVQSISTLALGIALSMYYEWSLGLVALAFTPFILIAFYMQRTLMAKENMGSAKTMENCTKLAVEVVSNIRTVASLGREEMFHQNYIGMLIPAVEISKRNTHFRGLVYGLARSLMFFAYAACMYYGTWCVIHRGIL-FGDVFKVSQALIMGTASIANALAFAPNMQKGVSAAKTIFTFLRRQPSIVDRP-GVSRDPWHSEGYVRFDKVKFSYPTRSEIQVLKGLELAVSKGQKIALVGPSGCGKSTCIQLIQRFYDVDEGATLIDECDVRNVSMTNLRNQLGIVSQEPILFDRTIRENISYGDNARNVTDQEIISACKKSNIHEFIANLPLGYDTRMGEKGAQLSGGQKQRIAIARALIRNPKIMLLDEATSALDAESEKVVQDALDAA-SEG-RTTISIAHRLSTVVHSD 1276          
BLAST of SMED30003432 vs. Ensembl Fly
Match: Mdr49 (gene:FBgn0004512 transcript:FBtr0087801)

HSP 1 Score: 363.229 bits (931), Expect = 1.648e-111
Identity = 212/580 (36.55%), Postives = 327/580 (56.38%), Query Frame = -1
Query:    1 SYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLV---------SP----------RDLTMTFSERMRPSVIK----FAILSSCTFISGFIQMVTISLTAAR-----QSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGS------YDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1635
            SY+ LFR++T  +  +++V ++ +    A  P  ++I+ +  +LLV         SP          + LT    E    ++I     F I S    ++ F+ ++T+++  A      Q  R+R  +  A+LRQ+I W+D        +K+T+D++ ++ GI +K+   +  I +FV  I+ AF  GWKL+ +    +P +    S++       + K++K+YS A  + EE+ S IRTV AFS QEKE +R+   L  AE  G K+ +  G+G+ +    I+   +L  + G+  I +        Y P  ++ +   +++G+  LG A P +E    A AA   LF +IDR  Q+    + G  PE+  G I F+ + F YP RP V IL+     +  G+TVAF+G SG GKST+I L+QRFYDP AG +   G DL+ +++ W RS IGVV QEP LF+ +I ENI+ G+  A   +I K+ + +N ++FI +  + ++T +G++GA  SGGQKQRIAIA ALVR PQ+LLLDEATSALD  SEK VQ  L+  +A    T ++VAHRL T+ NAD
Sbjct:   30 SYFDLFRYSTRCERFLLVVSLLVATAASAFIPYFMIIYGEFTSLLVDRTVGVGTSSPAFALPMFGGGQQLTNASKEENNQAIIDDATAFGIGSLVGSVAMFL-LITLAIDLANRIALNQIDRIRKLFLEAMLRQDIAWYDTSSGSNFASKMTEDLDKLKEGIGEKIVIVVFLIMTFVIGIVSAFVYGWKLTLVVLSCVPFIIAATSVVARLQGSLAEKELKSYSDAANVVEEVFSGIRTVFAFSGQEKEKERFGKLLIPAENTGRKKGLYSGMGNALSWLIIYLCMALAIWYGVTLILDERDLPDRVYTPAVLVIVLFAVIMGAQNLGFASPHVEAIAVATAAGQTLFNIIDRPSQVDPMDEKGNRPENTAGHIRFEGIRFRYPARPDVEILKGLTVDVLPGQTVAFVGASGCGKSTLIQLMQRFYDPEAGSVKLDGRDLRTLNVGWLRSQIGVVGQEPVLFATTIGENIRYGRPSATQADIEKAARAANCHDFITRLPKGYDTQVGEKGAQISGGQKQRIAIARALVRQPQVLLLDEATSALDPTSEKRVQSALE--LASQGPTTLVVAHRLSTITNAD 606          

HSP 2 Score: 271.937 bits (694), Expect = 1.151e-78
Identity = 176/526 (33.46%), Postives = 283/526 (53.80%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAV---PMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG---KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA FPL  L F D   +L S  D  +  +E ++ S+I F  +     +   +Q    +    + + RLR   F  ++ Q+I +FD ++   G L ++L  D  +++     ++   +Q +++ V  +++ F   W+ + +  +++P++  +  L   FI K + K   +   A  +A E +++IRTV     + + + +Y   +   +    ++   +GL   +     F    +  Y G + + E   +   +I++   ++ GS  LG A+   P + + I +      LF+     P      +  + +S  GDI ++NV FEYPTR G PILQ  N T+    TVA +GPSGSGKST + LL R+YDP++G ++ SG    +  L   RS +G+V QEP LF  +I ENI  G   + D   +EI+++ + SN + FI    Q ++T LG + +  SGGQKQRIAIA ALVRNP++L+LDEATSALD  SEK+VQ+ LD+  A   RT + +AHRL TVRNAD
Sbjct:  746 GATFPLWGLFFGDFFGIL-SDGDDDVVRAEVLKISMI-FVGIGLMAGLGNMLQTYMFTTAGVKMTTRLRKRAFGTIIGQDIAYFDDERNSVGALCSRLASDCSNVQGATGARVGTMLQAVATLVVGMVVGFVFSWQQTLLTLVTLPLVCLSVYLEGRFIMKSAQKAKASIEEASQVAVEAITNIRTVNGLCLERQVLDQYVQQIDRVDIACRRKVRFRGLVFALGQAAPFLAYGISMYYGGILVAEERMNYEDIIKVAEALIFGSWMLGQALAYAPNVNDAILSAGRLMDLFKRTSTQPNPPQSPYNTVEKS-EGDIVYENVGFEYPTRKGTPILQGLNLTIKKSTTVALVGPSGSGKSTCVQLLLRYYDPVSGSVNLSGVPSTEFPLDTLRSKLGLVSQEPVLFDRTIAENIAYGNNFRDDVSMQEIIEAAKKSNIHNFISALPQGYDTRLG-KTSQLSGGQKQRIAIARALVRNPKILILDEATSALDLESEKVVQQALDE--ARSGRTCLTIAHRLTTVRNAD 1265          
BLAST of SMED30003432 vs. Ensembl Fly
Match: Mdr65 (gene:FBgn0004513 transcript:FBtr0077011)

HSP 1 Score: 344.739 bits (883), Expect = 8.816e-105
Identity = 187/490 (38.16%), Postives = 282/490 (57.55%), Query Frame = -1
Query:    1 IKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEG------SYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS--HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1446
            I   I S   FISG   +   ++ A RQ  R+R   F++++RQ+I W D          + DDVE I  GIS+K+  F+  +  F+  + ++F+ GWKL+   S  IP++      +  F  K + ++ ++Y+ AG +AEEILSSIRTVV+F  ++ EV+RY + L  A      +    GL   V+   ++   +  ++ G+  I +        Y P  ++  F  I++G+  +    P LE+F TA+  A  LF+VID   +I        ++     GD+EF++V F YP+RP V + +  N  +  G+TVA +G SG GKST + LLQRFYDP+ G +     D++  ++ W RS+I VV QEP LF G+I +NI  GK  A  +EI  +   + A+EFI    + + + +G+RG+  SGGQKQRIAIA AL++NP++LLLDEATSALD  SEK VQ+ LD  +A   RT ++V+HRL  +R AD
Sbjct:  122 ILLTIASVVMFISGIFSVDVFNMVALRQVTRMRIKLFSSVIRQDIGWHDLASKQNFTQSMVDDVEKIRDGISEKVGHFVYLVVGFIITVAISFSYGWKLTLAVSSYIPLVILLNYYVAKFQGKLTAREQESYAGAGNLAEEILSSIRTVVSFGGEKSEVQRYENFLVPARKASQWKGAFSGLSDAVLKSMLYLSCAGAFWYGVNLIIDDRNVENKEYTPAILMIAFFGIIVGADNIARTAPFLESFATARGCATNLFKVIDLTSKIDPLSTDGKLLNYGLRGDVEFQDVFFRYPSRPEVIVHRGLNIRIRAGQTVALVGSSGCGKSTCVQLLQRFYDPVFGSVLLDDLDIRKYNIQWLRSNIAVVGQEPVLFLGTIAQNISYGKPGATQKEIEAAATQAGAHEFITNLPESYRSMIGERGSQLSGGQKQRIAIARALIQNPKILLLDEATSALDYQSEKQVQQALD--LASKGRTTIVVSHRLSAIRGAD 609          

HSP 2 Score: 287.73 bits (735), Expect = 2.464e-84
Identity = 179/535 (33.46%), Postives = 284/535 (53.08%), Query Frame = -1
Query:    1 VGAAFPLNVLIFRDVVNLLV--SPRDLTMTFSERMRPSVIKFAILSSCTFISG---FIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESF------YGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLK-DIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1557
            VG  +P   +IF +    L    P D         R +V+ +A L    F++G   F+Q    +      + R+R+  FNA++ QE+ WFD +    G L  +L+ +   I+  I   L+  IQ +S+F+  + +A    WKL+ +   + PI+  +  L    +    +++ +    A  IA E +++IRTV     +   ++ Y+  ++  E L  ++   +G+ +  +  + F   ++    G + + EG      +I++  T++ GS+ L  ++     F  A  A + LFQ++DR P+I     G I  +       +  + ++ + F YPTRP   IL   +  +  G+TVA +G SG GKST + LLQR+YDP  G I     D++ D+ L   R+ +G+V QEP LF  SI ENI  G  +      EI+ + + +NA+ FI+     ++T +G RG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE+LVQ+ LD T   G RT +++AHRL TV+NAD
Sbjct:  742 VGFLYPAFAVIFGEFYAALAEKDPEDALR------RTAVLSWACLG-LAFLTGLVCFLQTYLFNYAGIWLTTRMRAMTFNAMVNQEVGWFDDENNSVGALSARLSGEAVDIQGAIGYPLSGMIQALSNFISSVSVAMYYNWKLALLCLANCPIIVGSVILEAKMMSNAVVREKQVIEEACRIATESITNIRTVAGLRREADVIREYTEEIQRVEVLIRQKLRWRGVLNSTMQASAFFAYAVALCYGGVLVSEGQLPFQDIIKVSETLLYGSMMLAQSLAFTPAFSAALIAGHRLFQILDRKPKI-QSPMGTIKNTLAKQLNLFEGVRYRGIQFRYPTRPDAKILNGLDLEVLKGQTVALVGHSGCGKSTCVQLLQRYYDPDEGTIHIDHDDIQHDLTLDGVRTKLGIVSQEPTLFERSIAENIAYGDNRRSVSMVEIIAAAKSANAHSFIISLPNGYDTRMGARGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDLQSEQLVQQALD-TACSG-RTCIVIAHRLSTVQNAD 1266          
BLAST of SMED30003432 vs. Ensembl Fly
Match: CG10226 (gene:FBgn0035695 transcript:FBtr0077012)

HSP 1 Score: 334.724 bits (857), Expect = 4.057e-101
Identity = 198/584 (33.90%), Postives = 315/584 (53.94%), Query Frame = -1
Query:    4 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVS------PRDLTMTFS-----------------ERMRPSVIKFAILSSCTF----ISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMG--LLFIY--------EGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS--HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNA 1638
            VSY +LFR+  G D L+++   +++      FP+ ++++ ++V + +          +T+  S                 + +R   + F IL +        SG   +   +  A + + R+R  +F A LRQEI W D  +      ++TD++E I SGI++ L  +++ +   +  ++L+F  GWKL+      IP+     S + H+  K + ++  +Y  A  + EE++ +IRTVVAF  +  E  RY   LK A   G  +    GL   V+   +F  G+  ++ G  L+  Y        E  Y P  V+ +   I++ +  +    P LE F  A+ +A  +  VIDR   I    K   ++     G +EF++V F YP R  V +L+  N  +  G+TVA +GPSG GKST I LLQRFYDP+ G++   G D++  ++ W RS+I VV QEP LF GSI ENI+ GK +A  +E+  + + +NA++FI+   + ++T + ++G   SGGQ+QRIAIA AL++ P++LLLDEATSALD  SEKLVQ  LDK  A   RT ++V+HRL  +R+A
Sbjct:   36 VSYTELFRYIAGWDYLVLLSGFVAAFLQSLVFPIAIVVYSELVAMFIERTLGQGTSSVTIGLSLFGGGKILTNASYEENMQELRKDSVSFGILMTLNTLLMLFSGVYYVDAFNRLALKLTVRMRREFFKATLRQEIGWHDMAKDQNFAVRITDNMEKIRSGIAENLGHYVEIMCDVIISVVLSFIYGWKLALAIVFYIPLTLVVNSAVAHYQGKLTGQEQSSYVRASSVVEEVIGAIRTVVAFGGERSESLRYDSLLKPALKAGKWKGAFSGLSDTVMKAMLFITGAGSFWYGANLILYYRDPSIPIDEREYTPAVVMIVISGIIVSANQISRTSPFLETFAMARGSASAILDVIDRTSLIDPLSKAGKILNYGLKGAVEFRDVFFRYPAREDVIVLRGLNVVVEEGQTVALVGPSGCGKSTCIQLLQRFYDPVFGQVLLDGEDVRKYNIKWLRSNIAVVGQEPVLFQGSIGENIRHGKPEATQKEVEDAAKAANAHDFIIALHKGYDTDISEKGVQLSGGQRQRIAIARALIQQPKILLLDEATSALDYHSEKLVQAALDK--ACKGRTTLVVSHRLSAIRHA 617          

HSP 2 Score: 285.804 bits (730), Expect = 1.625e-83
Identity = 180/546 (32.97%), Postives = 287/546 (52.56%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISG----FIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQE-------KEVKRYSHNLKEAETLGVKRSICQGLGSGVI--GFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYG----------DIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLK-DIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKM--DAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G   P+  ++  ++   L  P D      E +       AI+S    I+     +IQ    +L     + R+RS  F  ++ QE+ WFD+++   G L  +L+ D  S++  I   L+  IQ  ++F+  I +AF   W+L+ I   + P M  +      F +K ++K+ +       IA E ++ IRTV     +E       KEV+RY H +         R +   LG  ++  G+ +    +L Y  G     +G     T+++I  T++ G   L  ++     F  A  +A  ++++IDR PQI   +   I ++  G           + ++ ++F YP+RP + +LQNFN  +  G+TVA +G SGSGKST + LL R+YDP  G+I      +  D+DL   R  +G+V QEP LF  SI +NI  G        ++I+++ + +NA+EFIM    +++T LG +G   SGGQKQRIAIA A+VRNP++LLLDEATSALD  SE++VQ+ LD   +   RT +++AHRL T++NA+
Sbjct:  755 GVTMPVFSVVLAELYGSLAKPTD------EEVLEQSASMAIISLVIGIAAGVVCYIQTFFFNLAGVWLTTRMRSKTFRCIMNQEMGWFDRKENSIGALSARLSGDAASVQGAIGFPLSNIIQAFTNFICSIAIAFPYSWELALICLSTSPFMVASIVFEARFGEKSALKEKEVLEETSRIATETITQIRTVAGLRREEELIKIYDKEVERYRHQILSRLKW---RGLVNSLGKSLMFFGYAV----TLTY--GGHMCADGKIKFETIMKISNTMLYGLFILAQSLAFTPAFNAALLSANRMYEIIDRKPQIQSPESFEIQQNGNGTAYKTNVVQQGVSYRGLNFSYPSRPHIKVLQNFNLDINQGQTVALVGASGSGKSTCVQLLMRYYDPDEGKILIDQESIHHDMDLKTLRRRLGIVSQEPSLFEKSIADNIGYGDTSRQVPMQQIIEAAKMANAHEFIMSLPAQYDTVLGSKGTQLSGGQKQRIAIARAMVRNPKILLLDEATSALDFQSERVVQQALDSACS--GRTCIVIAHRLSTIQNAN 1283          
BLAST of SMED30003432 vs. Ensembl Fly
Match: CG10226 (gene:FBgn0035695 transcript:FBtr0332068)

HSP 1 Score: 331.643 bits (849), Expect = 2.003e-100
Identity = 177/463 (38.23%), Postives = 267/463 (57.67%), Query Frame = -1
Query:    4 RLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMG--LLFIY--------EGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS--HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNA 1356
            R+R  +F A LRQEI W D  +      ++TD++E I SGI++ L  +++ +   +  ++L+F  GWKL+      IP+     S + H+  K + ++  +Y  A  + EE++ +IRTVVAF  +  E  RY   LK A   G  +    GL   V+   +F  G+  ++ G  L+  Y        E  Y P  V+ +   I++ +  +    P LE F  A+ +A  +  VIDR   I    K   ++     G +EF++V F YP R  V +L+  N  +  G+TVA +GPSG GKST I LLQRFYDP+ G++   G D++  ++ W RS+I VV QEP LF GSI ENI+ GK +A  +E+  + + +NA++FI+   + ++T + ++G   SGGQ+QRIAIA AL++ P++LLLDEATSALD  SEKLVQ  LDK  A   RT ++V+HRL  +R+A
Sbjct:   43 RMRREFFKATLRQEIGWHDMAKDQNFAVRITDNMEKIRSGIAENLGHYVEIMCDVIISVVLSFIYGWKLALAIVFYIPLTLVVNSAVAHYQGKLTGQEQSSYVRASSVVEEVIGAIRTVVAFGGERSESLRYDSLLKPALKAGKWKGAFSGLSDTVMKAMLFITGAGSFWYGANLILYYRDPSIPIDEREYTPAVVMIVISGIIVSANQISRTSPFLETFAMARGSASAILDVIDRTSLIDPLSKAGKILNYGLKGAVEFRDVFFRYPAREDVIVLRGLNVVVEEGQTVALVGPSGCGKSTCIQLLQRFYDPVFGQVLLDGEDVRKYNIKWLRSNIAVVGQEPVLFQGSIGENIRHGKPEATQKEVEDAAKAANAHDFIIALHKGYDTDISEKGVQLSGGQRQRIAIARALIQQPKILLLDEATSALDYHSEKLVQAALDK--ACKGRTTLVVSHRLSAIRHA 503          

HSP 2 Score: 285.419 bits (729), Expect = 9.465e-84
Identity = 181/545 (33.21%), Postives = 287/545 (52.66%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRD---LTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQE-------KEVKRYSHNLKEAETLGVKRSICQGLGSGVI--GFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYG----------DIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLK-DIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKM--DAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G   P+  ++  ++   L  P D   L  + S  +   VI  A    C   + F  +  + LT      R+RS  F  ++ QE+ WFD+++   G L  +L+ D  S++  I   L+  IQ  ++F+  I +AF   W+L+ I   + P M  +      F +K ++K+ +       IA E ++ IRTV     +E       KEV+RY H +         R +   LG  ++  G+ +    +L Y  G     +G     T+++I  T++ G   L  ++     F  A  +A  ++++IDR PQI   +   I ++  G           + ++ ++F YP+RP + +LQNFN  +  G+TVA +G SGSGKST + LL R+YDP  G+I      +  D+DL   R  +G+V QEP LF  SI +NI  G        ++I+++ + +NA+EFIM    +++T LG +G   SGGQKQRIAIA A+VRNP++LLLDEATSALD  SE++VQ+ LD   +   RT +++AHRL T++NA+
Sbjct:  641 GVTMPVFSVVLAELYGSLAKPTDEEVLEQSASMAIISLVIGIAAGVVCYIQTFFFNLAGVWLTT-----RMRSKTFRCIMNQEMGWFDRKENSIGALSARLSGDAASVQGAIGFPLSNIIQAFTNFICSIAIAFPYSWELALICLSTSPFMVASIVFEARFGEKSALKEKEVLEETSRIATETITQIRTVAGLRREEELIKIYDKEVERYRHQILSRLKW---RGLVNSLGKSLMFFGYAV----TLTY--GGHMCADGKIKFETIMKISNTMLYGLFILAQSLAFTPAFNAALLSANRMYEIIDRKPQIQSPESFEIQQNGNGTAYKTNVVQQGVSYRGLNFSYPSRPHIKVLQNFNLDINQGQTVALVGASGSGKSTCVQLLMRYYDPDEGKILIDQESIHHDMDLKTLRRRLGIVSQEPSLFEKSIADNIGYGDTSRQVPMQQIIEAAKMANAHEFIMSLPAQYDTVLGSKGTQLSGGQKQRIAIARAMVRNPKILLLDEATSALDFQSERVVQQALDSACS--GRTCIVIAHRLSTIQNAN 1169          
BLAST of SMED30003432 vs. Ensembl Zebrafish
Match: abcb4 (ATP-binding cassette, sub-family B (MDR/TAP), member 4 [Source:ZFIN;Acc:ZDB-GENE-080204-52])

HSP 1 Score: 451.44 bits (1160), Expect = 1.168e-144
Identity = 236/561 (42.07%), Postives = 350/561 (62.39%), Query Frame = -1
Query:    1 QLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVS------------------PRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1626
            +LFR+A  ID+L++++ +I S+  GA  PL V++F D+ +  V                   P    +T  E+M    I ++I+     ++ ++Q+   +L A RQ ++LR  +F+++++QEI WFD  +TGQL T+LTDDV  I  GI DKL   IQ +++F+  II+ F  GWKL+ +     P++  + +++   +  ++ K+  AY+ AG +AEE+LSSIRTV AF  Q+KE+KRY  NL++A+ +GV+++I   +  G   F I+   +L ++ G   I  G Y  G ++ IF  ++IG+  LG   P ++ F +A+ AA+ +FQ+ID  P+I S  + G   +   G+IEFKN+ F YP+R  V +L   N  +  G+T+A +G SG GKST I LLQRFYDP  G +S  G D++ +++   R  IGVV QEP LF+ +I ENI+ G+ D   +EI ++ +++NAY FIMK   KF T +GDRG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK      RT ++VAHRL T+RNAD
Sbjct:   43 ELFRYADSIDILLMMLGLIMSMANGAVLPLMVIVFGDMTDSFVDDTLLDNLKNITLPPNFTFPETSNITLGEKMTTHAIYYSIMGFVVLVAAYMQVAFWTLAAGRQVKKLRKIFFHSIMKQEIGWFDVNETGQLNTRLTDDVYKINEGIGDKLGMLIQNLTTFIVGIIIGFAKGWKLTLVILAVSPLLGISAAVIGKVMTTFTSKEQTAYAKAGAVAEEVLSSIRTVFAFGGQKKEIKRYHKNLEDAKNVGVRKAITVNIAMGFTFFMIYMSYALAFWYGSTLILGGEYTIGMLLTIFFAVLIGAFGLGQTSPNIQTFSSARGAAHKVFQIIDHEPKINSFSEEGYKLDVVKGNIEFKNIHFRYPSRDDVKVLNGMNLKVMSGQTIALVGSSGCGKSTTIQLLQRFYDPQEGSVSIDGHDIRSLNVRGLRELIGVVSQEPVLFATTIAENIRYGRQDVTQDEIEQAAREANAYNFIMKLPDKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQAALDKV--RLGRTTIVVAHRLSTIRNAD 601          

HSP 2 Score: 327.405 bits (838), Expect = 2.056e-98
Identity = 192/534 (35.96%), Postives = 291/534 (54.49%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNL--------KEAETLGVKRSICQGLGSGVIGFT---IFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHK-KHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G   P   +IF  ++ +   P D  +   +R     + FA +   +F + F+Q           + RLR   FNA++RQ++ W+D  K   G L T+L  D   ++     +LA   Q +++    I+++F  GW+L+ +    +PIMA   ++ +  +  +++K  K    AG IA E + ++RTVV+ + + K    Y  NL        K+A   G+  S  Q +    I F     F  GS +    L+  +EG      V  +   +V G++A+G A     N+  AK +A  +  +I+R P I +  + G  P+ F G++ F++V F+YP+RP VP+LQ     +  G+T+A +G SG GKST I LL+RFYDP  G +     D K +++ W RS IG+V QEP LF  S+ ENI  G    +   EEIV++ + +N + FI    Q++ T  GD+G   SGGQKQRIAIA A++RNP++LLLDEATSALD  SEK+VQ  LDK  A   RT ++VAHRL T++NAD
Sbjct:  721 GGMQPAFAVIFSKIIAVFAEP-DQNLV-RQRCDLYSLLFAGIGVLSFFTLFLQGFCFGKAGELLTMRLRFKAFNAMMRQDLAWYDDTKNSVGALTTRLAADTAQVQGATGVRLATLAQNVANLGTAIVISFVYGWQLTLLILSIVPIMAVAGAIQMKLLAGHALKDKKELEQAGKIATEAIENVRTVVSLTRESKFESLYEENLIVPYKNAKKKAHVFGLTFSFSQAM----IYFAYAGCFKFGSWLIEQKLM-TFEG------VFLVISAVVYGAMAVGEANSFTPNYAKAKMSASHVLMLINRAPAIDNSSEDGDKPDKFEGNVGFEHVYFKYPSRPDVPVLQGLKLRVKKGQTLALVGSSGCGKSTTIQLLERFYDPQQGRVMLDDNDAKQLNIHWLRSQIGIVSQEPVLFDCSLAENIAYGDNSREVDQEEIVEAAKAANIHSFIENLPQRYQTQAGDKGTQLSGGQKQRIAIARAILRNPKVLLLDEATSALDTESEKIVQDALDK--ASKGRTCIIVAHRLSTIQNAD 1239          
BLAST of SMED30003432 vs. Ensembl Zebrafish
Match: abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6414])

HSP 1 Score: 439.499 bits (1129), Expect = 1.188e-139
Identity = 238/604 (39.40%), Postives = 366/604 (60.60%), Query Frame = -1
Query:    1 KEKPAKSKTDIXXXXXXXXXXXXXXSVSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSP--------------RDLTMTFSER-----------MRPSVIK----FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMG--LLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRV-PQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1716
            +E P+ SK    + K  K  K  M+SV ++QLFR+AT  ++ ++++ ++ +   G A PL  ++F  + +  V                 + T T +             + P + K    F  +     + G  Q++   LTAA+Q++R+R  YF+A+L Q++ WFD    G+L  +LTDD+ +I  G+ DK+A F+Q   SF+  +++ F  GWKL+ +     P++A + ++    +   + K++ AY+ AG +AEEIL +IRTVVAF+ Q+K V++Y  NL EA+  GVK++I   +  G+  F +F   +L ++ G  L      +Y  G V+ +F +++IG+ +LG   P LE    A+ AAY +++ ID   P  S  K G  P+   GDIEFKN++F YP+R  V ILQ  +  +P+GKT+A +G SG GKST I LLQRFYDP +GE++  G D++ +++ W R ++G+V QEP LF  +I ENI+ G+ DA + +I ++ +++NAY+FI K   K NT +G+RGA  SGGQKQRIAIA ALV+NP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL T+R+AD
Sbjct:   41 EEPPSDSKHKGKKGKNSKDNKEPMKSVGFFQLFRYATCPEVFLMLIGLLCAAAHGVALPLMCVVFGQMTDSFVQSGQTYNLTGFNGNFTSNFTFTLNNTSTCLAGSPEIGIEPKMTKQAYFFIGIGGAVLVLGTFQVMLFLLTAAKQTKRIRQKYFHAILHQQMSWFDTHPIGELNIRLTDDINTINDGLGDKIAVFVQFFCSFISGLVIGFVFGWKLTLVILAVSPLLAGSAAVWSKILASLTSKELTAYAKAGAVAEEILVAIRTVVAFNGQKKAVEKYEKNLVEAKDFGVKKAISTNVSMGLTQFIVFATYALAFWYGTKLSVDEPENYTIGRVLTVFFSVMIGTFSLGQGAPNLEAIAKARGAAYEVYKTIDMPRPIDSSSKEGHKPDRVRGDIEFKNINFNYPSRKDVTILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDSGEVTLDGHDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDATDADIEQAIKEANAYDFISKLPDKLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSESIVQAALDK--ARAGRTTIVIAHRLSTIRSAD 642          

HSP 2 Score: 321.627 bits (823), Expect = 3.735e-96
Identity = 185/532 (34.77%), Postives = 289/532 (54.32%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSP-----RDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQ----GLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGK--MDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA +P   ++F  ++ +   P     R  TM FS       + + ++    F++ F Q      +    + RLRS  F A++RQEI WFD      G L TKL  D   ++     +L      I + +  +I+AF   W+L+ +    +P +     + +     ++ K   A  ++G I+ E + + +TVVA + ++     + H   ++ +   K S+C+    G+   +     + V + ++  G   I     +   V  +F  IV  ++ +G +     +F  AKAAA  + Q++++ P+I  + + G  P +F G+I+FK+V F YPTRP V +LQ  N ++  G+T+A +G SG GKST I LL+RFYDP  G++   G D K ++L W R+ +G+V QEP LF  +I ENIQ G        EEI ++ + +N + FI+    K+NT +GD+G   SGGQKQRIAIA ALVR P+LLLLDEATSALD  SEK+VQ  LD+  A   RT +++AHRL T++NAD
Sbjct:  783 GAVYPCVAILFAKIIGVFAEPDPEVKRQKTMMFS-------LLYLLIGVVAFLTYFFQGFMFGKSGELLTMRLRSQAFKAIVRQEIGWFDDNNNAVGILTTKLATDASLVKGAAGSRLGLATNTICALLIAVIVAFVFCWQLTLLILACVPFLTGANFIQLRATAGHTSKDQSALEMSGKISTETVENFKTVVALTREDV----FFHKFIDSLSTPYKASLCKAPIYGITFALAQAIPYLVNAAIFRFGAWLIAHCYTEYENVFLVFSVIVFAAMNIGQSSSFAPDFAKAKAAAGRIIQLLEKKPEIDIYDESGERPSTFSGNIDFKDVQFSYPTRPNVKVLQGLNVSVRQGQTLALVGSSGCGKSTTIQLLERFYDPAGGQVLVDGRDSKSVNLAWLRTQMGLVSQEPILFDCTISENIQYGDNSRTVTQEEIEEAAKKANIHNFILTLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRKPKLLLLDEATSALDTESEKIVQAALDE--ARLGRTCIVIAHRLTTIQNAD 1301          
BLAST of SMED30003432 vs. Ensembl Zebrafish
Match: abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6414])

HSP 1 Score: 439.884 bits (1130), Expect = 1.297e-139
Identity = 237/604 (39.24%), Postives = 366/604 (60.60%), Query Frame = -1
Query:    1 KEKPAKSKTDIXXXXXXXXXXXXXXSVSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSP--------------RDLTMTFSER-----------MRPSVIK----FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEG--SYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRV-PQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1716
            +E P+ SK    + K  K  K  M+SV ++QLFR+AT  ++ ++++ ++ +   G A PL  ++F  + +  V                 + T T +             + P + K    F  +     + G  Q++   LTAA+Q++R+R  YF+A+L Q++ WFD    G+L  +LTDD+ +I  G+ DK+A F+Q   SF+  +++ F  GWKL+ +     P++A + ++    +   + K++ AY+ AG +AEEIL +IRTVVAF+ Q+K V++Y  NL EA+  GVK++I   +  G+  F +F   +L ++ G     +   +Y  G V+ +F +++IG+ +LG   P LE    A+ AAY +++ ID   P  S  K G  P+   GDIEFKN++F YP+R  V ILQ  +  +P+GKT+A +G SG GKST I LLQRFYDP +GE++  G D++ +++ W R ++G+V QEP LF  +I ENI+ G+ DA + +I ++ +++NAY+FI K   K NT +G+RGA  SGGQKQRIAIA ALV+NP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL T+R+AD
Sbjct:   53 EEPPSDSKHKGKKGKNSKDNKEPMKSVGFFQLFRYATCPEVFLMLIGLLCAAAHGVALPLMCVVFGQMTDSFVQSGQTYNLTGFNGNFTSNFTFTLNNTSTCLAGSPEIGIEPKMTKQAYFFIGIGGAVLVLGTFQVMLFLLTAAKQTKRIRQKYFHAILHQQMSWFDTHPIGELNIRLTDDINTINDGLGDKIAVFVQFFCSFISGLVIGFVFGWKLTLVILAVSPLLAGSAAVWSKILASLTSKELTAYAKAGAVAEEILVAIRTVVAFNGQKKAVEKYEKNLVEAKDFGVKKAISTNVSMGLTQFIVFATYALAFWYGTKLSVDEPENYTIGRVLTVFFSVMIGTFSLGQGAPNLEAIAKARGAAYEVYKTIDMPRPIDSSSKEGHKPDRVRGDIEFKNINFNYPSRKDVTILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDSGEVTLDGHDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDATDADIEQAIKEANAYDFISKLPDKLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSESIVQAALDK--ARAGRTTIVIAHRLSTIRSAD 654          

HSP 2 Score: 322.398 bits (825), Expect = 2.423e-96
Identity = 187/533 (35.08%), Postives = 290/533 (54.41%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSP-----RDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTI-----FTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA +P   ++F  ++ +   P     R  TM FS       + + ++    F++ F Q      +    + RLRS  F A++RQEI WFD      G L TKL  D   ++     +L      I + +  +I+AF   W+L+ +    +P +     + +     ++ K   A  ++G I+ E + + +TVVA + ++     + H   ++ +   K S+C+    G I F +     + V + ++  G   I     +   V  +F  IV  ++ +G +     +F  AKAAA  + Q++++ P+I  + + G  P +F G+I+FK+V F YPTRP V +LQ  N ++  G+T+A +G SG GKST I LL+RFYDP  G++   G D K ++L W R+ +G+V QEP LF  +I ENIQ G        EEI ++ + +N + FI+    K+NT +GD+G   SGGQKQRIAIA ALVR P+LLLLDEATSALD  SEK+VQ  LD+  A   RT +++AHRL T++NAD
Sbjct:  795 GAVYPCVAILFAKIIGVFAEPDPEVKRQKTMMFS-------LLYLLIGVVAFLTYFFQGFMFGKSGELLTMRLRSQAFKAIVRQEIGWFDDNNNAVGILTTKLATDASLVKGAAGSRLGLATNTICALLIAVIVAFVFCWQLTLLILACVPFLTGANFIQLRATAGHTSKDQSALEMSGKISTETVENFKTVVALTREDV----FFHKFIDSLSTPYKASLCKAPIYG-ITFALAQAIPYLVNAAIFRFGAWLIAHCYTEYENVFLVFSVIVFAAMNIGQSSSFAPDFAKAKAAAGRIIQLLEKKPEIDIYDESGERPSTFSGNIDFKDVQFSYPTRPNVKVLQGLNVSVRQGQTLALVGSSGCGKSTTIQLLERFYDPAGGQVLVDGRDSKSVNLAWLRTQMGLVSQEPILFDCTISENIQYGDNSRTVTQEEIEEAAKKANIHNFILTLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRKPKLLLLDEATSALDTESEKIVQAALDE--ARLGRTCIVIAHRLTTIQNAD 1313          
BLAST of SMED30003432 vs. Ensembl Zebrafish
Match: abcb11a (ATP-binding cassette, sub-family B (MDR/TAP), member 11a [Source:ZFIN;Acc:ZDB-GENE-050517-13])

HSP 1 Score: 407.527 bits (1046), Expect = 1.051e-127
Identity = 239/621 (38.49%), Postives = 359/621 (57.81%), Query Frame = -1
Query:    1 YNVEEANFANSILKEKPAKSKTDIXXXXXXXXXXXXXXSVSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVS------------------------------PRDLTMTFSERMRPSVIKFAI----LSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEG-SYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1755
            YN E+A+  N  +K+K  K+ T                 V ++QLFRF+T  ++L+++V    S+  GAA PL +L++  + N  V                               P + T+     +   +  FA+    +     I  F Q+      AARQ +R+R  YF  ++  EI WFD    G+L T+++DD+  I + I+D+++ FI++IS+F+   ++ F  GWKL+ +     P++     LM   + + + +++KAY+ AG +A+E+LSSIRTV AF  + KE +RY  NL +A+  G+K+ +  G+  G +   IF   +L ++ G   + E     PG ++Q+F  ++IG++ LG A P LE F + +AAA  +F  IDR P+I      G   +   GDIEF +V+F YP+RP V IL + N  +  G+T AF+GPSGSGK+T I L+QRFYDP  G +S  G D++ +++ W RS IGVV+QEP LF+ +I ENI+ G+     +EI+++ + +NAY FIM   Q F+T +G+ G   SGGQKQRIAIA ALVRNP++LLLD ATSALDN SE +VQ+ LDK  A   RT + +AHRL T+RNAD
Sbjct:   17 YNAEDADLKNGEVKKKE-KAPT-----------------VGFFQLFRFSTWREVLMMVVGSFCSLVHGAATPLMLLVYGMMTNTFVEYEVEILELTDPNKTCINNTISWMNGSAVQRPDNTTIYCGVDIEAEMTNFALYYIGIGVGVLILSFFQITFWVSAAARQIQRIRKTYFRKIMCMEIGWFDCNSVGELNTRMSDDINKINNAIADQVSIFIERISTFIFGFMVGFIGGWKLTLVVIAVSPLLGLAAGLMAMAVARLTGRELKAYAKAGAVADEVLSSIRTVAAFGGEHKEAERYDRNLVQAQEWGIKKGMIIGVFQGYLWCIIFLCYALAFWFGSKLVIETQELTPGGLVQVFFGVLIGAMNLGQASPCLEAFASGRAAAKSIFDTIDREPEIDCFSDEGHTLDKVKGDIEFHSVNFNYPSRPEVKILDDLNIVVKAGETTAFVGPSGSGKTTTIQLIQRFYDPSEGMVSLDGHDIRSLNIQWLRSLIGVVEQEPVLFATTIAENIRYGRAGVTMQEIIEAAKQANAYNFIMSLPQTFDTLVGEGGGQMSGGQKQRIAIARALVRNPRILLLDMATSALDNESEAVVQEALDK--ARQGRTTISIAHRLSTIRNAD 617          

HSP 2 Score: 288.115 bits (736), Expect = 3.055e-84
Identity = 175/524 (33.40%), Postives = 278/524 (53.05%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDK--QQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVY-YMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G+  P+  L+F  ++     P         ++    I F ++   +F S F+Q  + + +    +RRLR   F A+L+QEI WFD      G L T+L  +   ++     ++   +  +++     I+A+   WKLS + +  +P++  +       +   + +   A   AG ++ E +S+IRT+   + ++  V ++   L+       K++   G+        IF   +  + Y G L  +EG      V ++   +V  + ALG A     ++  AK +A  LFQ++DRVP+I+  K  G     F G +EFK   F YP+RP V +L+    ++  G+T+AF+G SG GKST + LL+RFYDP  G++   G     I + + RS IG+V QEP LF  SI ENIQ G        EEI+ + + +  ++F+M    K+ T +G +G+  S GQKQRIAIA A+VRNP++LLLDEATSALD  SEK VQ  LD+  A   RT +++AHRL T+++AD
Sbjct:  765 GSLNPMYALLFSQILGTFSIPDPDDQ--RRQINGICILFVVIGVVSFFSQFLQGYSFAKSGELLTRRLRKFGFQAMLKQEIGWFDDPMNSPGALTTRLATNASMVQGATGSQIGMIVNSLTNIGASFIIAYYFSWKLSLVVTCFLPLIGLSGVFQSKMLTGLANEDKTALEAAGQVSSEAMSNIRTIAGLAKEKHFVAQFEKQLQAPYKAAKKKAYVYGICFAFARCVIFMAYAASFRYGGYLVSHEG-LQYMMVFRVISALVTSATALGRASSFTPDYAKAKISAAQLFQLLDRVPKINVSKTEGQSWNDFKGKVEFKGCRFTYPSRPDVQVLRGLVVSVHPGQTLAFVGSSGCGKSTSVQLLERFYDPDEGQVLIDGRPSDSISVPFLRSQIGIVSQEPVLFDCSIAENIQYGDNSRTVSMEEIIDAAKKAYLHDFVMTLPDKYETQVGAQGSQLSRGQKQRIAIARAIVRNPKILLLDEATSALDTESEKTVQAALDE--ARQGRTCIVIAHRLSTIQSAD 1283          
BLAST of SMED30003432 vs. Ensembl Zebrafish
Match: abcb11b (ATP-binding cassette, sub-family B (MDR/TAP), member 11b [Source:ZFIN;Acc:ZDB-GENE-050517-14])

HSP 1 Score: 396.741 bits (1018), Expect = 5.795e-124
Identity = 206/474 (43.46%), Postives = 304/474 (64.14%), Query Frame = -1
Query:    1 FISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGL-LFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISH-KKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1416
            FI G++Q+      AARQ + +R  YF  ++R EI WFD    G+L T+++DD+  I   I+D++  FIQ+ ++FV   ++ F  GWKL+ +     P++     LM  F+ K +  +++AY+ AG +A+E+LSS+RTV AF  ++KEV RY  NL  A+  G+++ +  G  +G + F IF   +L ++ G  L +    Y PGT++Q+F  ++I ++ LG A P LE F   + AA  +F+ IDR P+I    + G   +   GD+EF N+ F YP+RP V IL   N  +  G+T AF+GPSG+GKST I L+QRFYDP  G ++  G D++ +++ W RS IG+V+QEP LF+ +I ENI+ G+    N++I+ + +++NAY FIM   QKF T +G+ G   SGGQKQRIAIA ALVRNP++LLLD ATSALDN SE +VQ+ LDK      RT + +AHRL T++NAD
Sbjct:   95 FILGYLQISLWITAAARQIQIIRKMYFRKVMRMEIGWFDCTSVGELNTRMSDDINKINDAIADQVGIFIQRFTTFVCGFLMGFARGWKLTLVIISVSPLIGIGAGLMALFVAKLTGMELQAYAKAGAVADEVLSSVRTVAAFGGEKKEVDRYDRNLISAQQWGIRKGLIMGFFTGYMWFIIFLCYALAFWYGSSLVVDTQEYSPGTLLQVFFGVLIAALNLGQASPCLEAFAAGRGAATIIFETIDREPEIDCLSEAGYKLDKVKGDLEFHNITFHYPSRPEVKILDQLNLQVKSGETTAFVGPSGAGKSTAIQLIQRFYDPKEGMLTLDGHDIRGLNIQWLRSLIGIVEQEPVLFATTIAENIRYGRPGVSNDDIITAAKEANAYNFIMDLPQKFETLVGEGGGQMSGGQKQRIAIARALVRNPRILLLDMATSALDNESEAVVQEALDKV--RMGRTTISIAHRLSTIKNAD 566          

HSP 2 Score: 277.715 bits (709), Expect = 9.802e-81
Identity = 166/485 (34.23%), Postives = 269/485 (55.46%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F ++   +F +  +Q    S +    +RRLR   F A+L QEI WFD  K   G L T+L  D   ++     ++   +  +++    +I++F   WKL+ +    +P +A +       +  ++ +  +A   AG I+ E L++IRT+     +   V+ +   L+      +K++   G   G     +F   S  Y  G   +Y        V ++   IV    ALG A     ++  AK +A   FQ++DR+P+IS + K G   ++F GDIEF +  F YP+RP + +L   N ++  G+T+AF+G SG GKST + LL+RFYDP +G +   G +   I++ + RS IG+V QEP LF  SI ENI+ G  + +    +++ + + +  ++F+M   +K++T +G +G+  S GQKQRIAIA A++R+P++LLLDEATSALD  SEK VQ+ LDK  A   RT +++AHRL T++N+D
Sbjct:  760 FVVVGLVSFFTQMLQGYAFSKSGELLTRRLRRLGFQAMLGQEIGWFDDRKNSPGALTTRLATDASQVQGATGSQIGMIVNSLTNIGVAVIISFYFSWKLTLVILCFLPFLALSGGFQAKMLTGFAKQDKEAMETAGQISGEALNNIRTIAGLGKERNFVEMFETQLEAPYQAALKKANVYGACYGFAQCVVFMANSASYRFGGYLVYHEGLHFSFVFRVISAIVTSGTALGRASSYTPDYAKAKISAARFFQLLDRIPKISVYSKDGQKWDNFKGDIEFIDCKFTYPSRPDIQVLNGLNVSVKPGQTLAFVGSSGCGKSTSVQLLERFYDPNSGRVLIDGRESSQINVAYLRSKIGIVSQEPILFDCSIAENIRYGDNQRELSMNDVISAAKKAQLHDFVMSLPEKYDTNVGSQGSQLSRGQKQRIAIARAIIRDPKILLLDEATSALDTESEKTVQEALDK--AREGRTCIVIAHRLSTIQNSD 1242          
BLAST of SMED30003432 vs. Ensembl Xenopus
Match: mia2 (MIA SH3 domain ER export factor 2 [Source:Xenbase;Acc:XB-GENE-993630])

HSP 1 Score: 469.929 bits (1208), Expect = 1.381e-151
Identity = 240/558 (43.01%), Postives = 354/558 (63.44%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDL-----------TMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V  + +FR+++  D ++++   I+SI  GAA PL +++F D+ +  V+   +           +    E+M      ++ L     +  ++Q+   +L+A +Q +R+RSN+F+A+LRQEI WFD    G+L T+LTDDV  I  GI DK+A  +Q +++FV   I+ F  GWKL+ +     P+M  + ++    +  ++ K++KAY+ AG +AEE+LSSIRTV AF  Q+KE+KRY  NL+EA+ +G+K++I   +  G     I+   +L ++ G   I  G Y  G+V+ +F  ++IG+ A+G   P +E F  A+ AAY +F +ID  P+I S  K G+ P+   GDIEFKNV F YP+R  + +L+  N  +P GKTVA +G SG GKST + L+QRFYDP  G I+  G D++ +++ + R  IGVV QEP LF  +I +NI+ G+ D   EEI ++T+++NAY FIMK   KF T +G+RG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT ++VAHRL T+RNAD
Sbjct:   48 VGVFTMFRYSSTSDRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVGNFSLEALINASRNLQEQMTTYAYYYSGLGFGVLLFAYVQISFWTLSAGKQIKRIRSNFFHAVLRQEIGWFDVNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIINGGYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEGLKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGREDVTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDK--AREGRTTIVVAHRLSTIRNAD 603          

HSP 2 Score: 318.546 bits (815), Expect = 3.835e-95
Identity = 183/527 (34.72%), Postives = 286/527 (54.27%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAIL----SSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA  P   +IF  ++ +   P       +  MR     +++L       +FI+ F+Q+            R    YF     +EI WFD  K  TG L T+L  D   ++     +LA   Q I++    +I++F  GW+L+ +    +PI+A +  + +     ++ K  K    AG I+ + + +IRTVV+ + + K    Y  NL+      +K++   GL   +    ++   +  +  G   + E    P  V  +F  IV G++ALG       ++  A  +A  +F +++RVP I S+ K G  P++  G+++F+ V F YPTRP + +LQ  + ++  G+T+A +G SG GKST + LL+RFYDP  GE+   G  ++D+++ W R+ +G+V QEP LF  SI ENI  G    +    EI  + +++N + FI     K+NT +GD+G   SGGQKQRIAIA ALVR P++LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  722 GATQPAFAIIFSKIIGVFAGP-------ASEMRAKSTMYSLLFLALGGISFITFFLQV------------RFGHVYFTL---KEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYEKNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAELLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERVPLIDSYSKEGEKPQNCGGNVQFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDK--ARTGRTCIVIAHRLSTIQNAD 1224          
BLAST of SMED30003432 vs. Ensembl Xenopus
Match: mia2 (MIA SH3 domain ER export factor 2 [Source:Xenbase;Acc:XB-GENE-993630])

HSP 1 Score: 470.315 bits (1209), Expect = 1.460e-151
Identity = 240/558 (43.01%), Postives = 354/558 (63.44%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDL-----------TMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V  + +FR+++  D ++++   I+SI  GAA PL +++F D+ +  V+   +           +    E+M      ++ L     +  ++Q+   +L+A +Q +R+RSN+F+A+LRQEI WFD    G+L T+LTDDV  I  GI DK+A  +Q +++FV   I+ F  GWKL+ +     P+M  + ++    +  ++ K++KAY+ AG +AEE+LSSIRTV AF  Q+KE+KRY  NL+EA+ +G+K++I   +  G     I+   +L ++ G   I  G Y  G+V+ +F  ++IG+ A+G   P +E F  A+ AAY +F +ID  P+I S  K G+ P+   GDIEFKNV F YP+R  + +L+  N  +P GKTVA +G SG GKST + L+QRFYDP  G I+  G D++ +++ + R  IGVV QEP LF  +I +NI+ G+ D   EEI ++T+++NAY FIMK   KF T +G+RG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT ++VAHRL T+RNAD
Sbjct:   48 VGVFTMFRYSSTSDRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVGNFSLEALINASRNLQEQMTTYAYYYSGLGFGVLLFAYVQISFWTLSAGKQIKRIRSNFFHAVLRQEIGWFDVNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIINGGYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEGLKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGREDVTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDK--AREGRTTIVVAHRLSTIRNAD 603          

HSP 2 Score: 338.191 bits (866), Expect = 2.817e-102
Identity = 188/527 (35.67%), Postives = 293/527 (55.60%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAIL----SSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA  P   +IF  ++ +   P       +  MR     +++L       +FI+ F+Q  T        ++RLR   F ++LRQEI WFD  K  TG L T+L  D   ++     +LA   Q I++    +I++F  GW+L+ +    +PI+A +  + +     ++ K  K    AG I+ + + +IRTVV+ + + K    Y  NL+      +K++   GL   +    ++   +  +  G   + E    P  V  +F  IV G++ALG       ++  A  +A  +F +++RVP I S+ K G  P++  G+++F+ V F YPTRP + +LQ  + ++  G+T+A +G SG GKST + LL+RFYDP  GE+   G  ++D+++ W R+ +G+V QEP LF  SI ENI  G    +    EI  + +++N + FI     K+NT +GD+G   SGGQKQRIAIA ALVR P++LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  720 GATQPAFAIIFSKIIGVFAGP-------ASEMRAKSTMYSLLFLALGGISFITFFLQGFTFGKAGEILTKRLRLWSFKSMLRQEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYEKNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAELLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERVPLIDSYSKEGEKPQNCGGNVQFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDK--ARTGRTCIVIAHRLSTIQNAD 1237          
BLAST of SMED30003432 vs. Ensembl Xenopus
Match: mia2 (MIA SH3 domain ER export factor 2 [Source:Xenbase;Acc:XB-GENE-993630])

HSP 1 Score: 469.159 bits (1206), Expect = 4.276e-151
Identity = 240/560 (42.86%), Postives = 354/560 (63.21%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDL-------------TMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V  + +FR+++  D ++++   I+SI  GAA PL +++F D+ +  V+   +             +    E+M      ++ L     +  ++Q+   +L+A +Q +R+RSN+F+A+LRQEI WFD    G+L T+LTDDV  I  GI DK+A  +Q +++FV   I+ F  GWKL+ +     P+M  + ++    +  ++ K++KAY+ AG +AEE+LSSIRTV AF  Q+KE+KRY  NL+EA+ +G+K++I   +  G     I+   +L ++ G   I  G Y  G+V+ +F  ++IG+ A+G   P +E F  A+ AAY +F +ID  P+I S  K G+ P+   GDIEFKNV F YP+R  + +L+  N  +P GKTVA +G SG GKST + L+QRFYDP  G I+  G D++ +++ + R  IGVV QEP LF  +I +NI+ G+ D   EEI ++T+++NAY FIMK   KF T +G+RG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT ++VAHRL T+RNAD
Sbjct:   48 VGVFTMFRYSSTSDRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVGSGNFSLEALINASRNLQEQMTTYAYYYSGLGFGVLLFAYVQISFWTLSAGKQIKRIRSNFFHAVLRQEIGWFDVNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIINGGYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEGLKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGREDVTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDK--AREGRTTIVVAHRLSTIRNAD 605          

HSP 2 Score: 337.806 bits (865), Expect = 4.417e-102
Identity = 188/527 (35.67%), Postives = 293/527 (55.60%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAIL----SSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA  P   +IF  ++ +   P       +  MR     +++L       +FI+ F+Q  T        ++RLR   F ++LRQEI WFD  K  TG L T+L  D   ++     +LA   Q I++    +I++F  GW+L+ +    +PI+A +  + +     ++ K  K    AG I+ + + +IRTVV+ + + K    Y  NL+      +K++   GL   +    ++   +  +  G   + E    P  V  +F  IV G++ALG       ++  A  +A  +F +++RVP I S+ K G  P++  G+++F+ V F YPTRP + +LQ  + ++  G+T+A +G SG GKST + LL+RFYDP  GE+   G  ++D+++ W R+ +G+V QEP LF  SI ENI  G    +    EI  + +++N + FI     K+NT +GD+G   SGGQKQRIAIA ALVR P++LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  730 GATQPAFAIIFSKIIGVFAGP-------ASEMRAKSTMYSLLFLALGGISFITFFLQGFTFGKAGEILTKRLRLWSFKSMLRQEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYEKNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAELLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERVPLIDSYSKEGEKPQNCGGNVQFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDK--ARTGRTCIVIAHRLSTIQNAD 1247          
BLAST of SMED30003432 vs. Ensembl Xenopus
Match: mia2 (MIA SH3 domain ER export factor 2 [Source:Xenbase;Acc:XB-GENE-993630])

HSP 1 Score: 468.003 bits (1203), Expect = 6.877e-151
Identity = 239/557 (42.91%), Postives = 353/557 (63.38%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPS----------VIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V  + +FR+++  D ++++   I+SI  GAA PL +++F D+ +  V+   +    +   R +             ++ L     +  ++Q+   +L+A +Q +R+RSN+F+A+LRQEI WFD    G+L T+LTDDV  I  GI DK+A  +Q +++FV   I+ F  GWKL+ +     P+M  + ++    +  ++ K++KAY+ AG +AEE+LSSIRTV AF  Q+KE+KRY  NL+EA+ +G+K++I   +  G     I+   +L ++ G   I  G Y  G+V+ +F  ++IG+ A+G   P +E F  A+ AAY +F +ID  P+I S  K G+ P+   GDIEFKNV F YP+R  + +L+  N  +P GKTVA +G SG GKST + L+QRFYDP  G I+  G D++ +++ + R  IGVV QEP LF  +I +NI+ G+ D   EEI ++T+++NAY FIMK   KF T +G+RG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT ++VAHRL T+RNAD
Sbjct:   48 VGVFTMFRYSSTSDRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVAFHSTAHARANDTGRRKRCRYAYYYSGLGFGVLLFAYVQISFWTLSAGKQIKRIRSNFFHAVLRQEIGWFDVNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIINGGYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEGLKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGREDVTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDK--AREGRTTIVVAHRLSTIRNAD 602          

HSP 2 Score: 317.39 bits (812), Expect = 7.755e-95
Identity = 182/526 (34.60%), Postives = 282/526 (53.61%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAIL----SSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA  P   +IF  ++ +   P       +  MR     +++L       +FI+ F+Q  T        ++RLR   F ++LRQEI WFD  K  TG L T+L  D   ++     +LA   Q I++    +I++F  GW+L+ +    +PI+A +  + +     ++ K  K    AG I+ + + +IRTVV+ + + K    Y  NL+      +K++   GL   +    ++   +  +  G   + E    P  V  +F  IV G++ALG       ++  A  +A  +F +++R+                G I F+ V F YPTRP + +LQ  + ++  G+T+A +G SG GKST + LL+RFYDP  GE+   G  ++D+++ W R+ +G+V QEP LF  SI ENI  G    +    EI  + +++N + FI     K+NT +GD+G   SGGQKQRIAIA ALVR P++LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  719 GATQPAFAIIFSKIIGVFAGP-------ASEMRAKSTMYSLLFLALGGISFITFFLQGFTFGKAGEILTKRLRLWSFKSMLRQEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYEKNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAELLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERLG---------------GYITFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDK--ARTGRTCIVIAHRLSTIQNAD 1220          
BLAST of SMED30003432 vs. Ensembl Xenopus
Match: mia2 (MIA SH3 domain ER export factor 2 [Source:Xenbase;Acc:XB-GENE-993630])

HSP 1 Score: 467.233 bits (1201), Expect = 1.426e-150
Identity = 242/553 (43.76%), Postives = 355/553 (64.20%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDV------VNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V  + +FR+++  D ++++   I+SI  GAA PL +++F D+      V  +VSP + T    ++       ++ L     +  ++Q+   +L+A +Q +R+RSN+F+A+LRQEI WFD    G+L T+LTDDV  I  GI DK+A  +Q +++FV   I+ F  GWKL+ +     P+M  + ++    +  ++ K++KAY+ AG +AEE+LSSIRTV AF  Q+KE+KRY  NL+EA+ +G+K++I   +  G     I+   +L ++ G   I  G Y  G+V+ +F  ++IG+ A+G   P +E F  A+ AAY +F +ID  P+I S  K G+ P+   GDIEFKNV F YP+R  + +L+  N  +P GKTVA +G SG GKST + L+QRFYDP  G I+  G D++ +++ + R  IGVV QEP LF  +I +NI+ G+ D   EEI ++T+++NAY FIMK   KF T +G+RG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT ++VAHRL T+RNAD
Sbjct:   48 VGVFTMFRYSSTSDRILMLFGTIASISHGAALPLMMIVFGDMTDSFVNVGQVVSP-NFTCLDDQKY---AYYYSGLGFGVLLFAYVQISFWTLSAGKQIKRIRSNFFHAVLRQEIGWFDVNDAGELNTRLTDDVSKINEGIGDKIAMLLQSLTTFVTGFIIGFVKGWKLTLVILAISPVMGFSAAIWAKVLSAFTNKELKAYAKAGAVAEEVLSSIRTVFAFGGQDKEIKRYEKNLEEAKRIGIKKAITANVSIGFAFLMIYAAYALAFWYGTTLIINGGYTIGSVLTVFFAVIIGAFAVGQTSPNVEAFANARGAAYTIFNIIDNQPKIDSFSKEGLKPDKIKGDIEFKNVKFTYPSRTDIQVLKGLNLNIPSGKTVALVGSSGCGKSTTVQLIQRFYDPEEGVITLDGQDIRSLNIRYLREIIGVVSQEPILFDTTIADNIRYGREDVTMEEIERATKEANAYNFIMKLPDKFETLVGERGTQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESESVVQAALDK--AREGRTTIVVAHRLSTIRNAD 594          

HSP 2 Score: 337.806 bits (865), Expect = 3.142e-102
Identity = 188/527 (35.67%), Postives = 293/527 (55.60%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAIL----SSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA  P   +IF  ++ +   P       +  MR     +++L       +FI+ F+Q  T        ++RLR   F ++LRQEI WFD  K  TG L T+L  D   ++     +LA   Q I++    +I++F  GW+L+ +    +PI+A +  + +     ++ K  K    AG I+ + + +IRTVV+ + + K    Y  NL+      +K++   GL   +    ++   +  +  G   + E    P  V  +F  IV G++ALG       ++  A  +A  +F +++RVP I S+ K G  P++  G+++F+ V F YPTRP + +LQ  + ++  G+T+A +G SG GKST + LL+RFYDP  GE+   G  ++D+++ W R+ +G+V QEP LF  SI ENI  G    +    EI  + +++N + FI     K+NT +GD+G   SGGQKQRIAIA ALVR P++LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  710 GATQPAFAIIFSKIIGVFAGP-------ASEMRAKSTMYSLLFLALGGISFITFFLQGFTFGKAGEILTKRLRLWSFKSMLRQEIGWFDDTKNSTGALTTRLATDASQVQGATGSRLALLAQNIANLGTAVIISFIYGWRLTLLILAIVPIIAASALVQLKMFAGHAKKDKKELEKAGKISTDAVLNIRTVVSLTRERKFEAMYEKNLEGPYRNSIKKAHLHGLTYALSQAIMYFAYAGCFRFGAYLVAELLMTPDDVFLVFSAIVFGAMALGQTSSFAPDYTKAMISAAHIFSLLERVPLIDSYSKEGEKPQNCGGNVQFQGVQFNYPTRPDITVLQGLDISVKQGETLALVGSSGCGKSTTVSLLERFYDPFGGEVLVDGHSVRDLNIQWVRAQMGIVSQEPILFDCSIGENIAYGDNSREVTQAEIETAAKEANIHSFIDTLTDKYNTRVGDKGTQMSGGQKQRIAIARALVRKPKILLLDEATSALDTESEKVVQEALDK--ARTGRTCIVIAHRLSTIQNAD 1227          
BLAST of SMED30003432 vs. Ensembl Mouse
Match: Abcb1a (ATP-binding cassette, sub-family B (MDR/TAP), member 1A [Source:MGI Symbol;Acc:MGI:97570])

HSP 1 Score: 450.284 bits (1157), Expect = 4.764e-144
Identity = 234/562 (41.64%), Postives = 348/562 (61.92%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVS---------------PRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS   +FR+A  +D L ++V  +++I  G A PL +LIF D+ +   S                R +     E M      +  + +   I  +IQ+    L A RQ  ++R  +F+A++ QEI WFD    G+L T+LTDDV  I  GI DK+  F Q +++F G  I+ FT GWKL+ +     P++  +  +    +  ++ K++ AY+ AG +AEE+L++IRTV+AF  Q+KE++RY++NL+EA+ LG+K++I   +  G     I+   +L ++ G   +    Y  G V+ +F +++IG+ ++G A P +E F  A+ AAY +F++ID  P I S  K G  P++  G++EFKN+ F YP+R  V IL+  N  +  G+TVA +G SG GKST + L+QR YDPL G +S  G D++ I++ + R  IGVV QEP LF+ +I ENI+ G+ D   +EI K+ +++NAY+FIMK   +F+T +G+RGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:   33 VSVLTMFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLIFGDMTDSFASVGNVSKNSTNMSEADKRAMFAKLEEEMTTYAYYYTGIGAGVLIVAYIQVSFWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFSVLIGAFSVGQASPNIEAFANARGAAYEVFKIIDNKPSIDSFSKSGHKPDNIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDK--AREGRTTIVIAHRLSTVRNAD 592          

HSP 2 Score: 328.946 bits (842), Expect = 5.104e-99
Identity = 178/493 (36.11%), Postives = 282/493 (57.20%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNL--------KEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F IL   +FI+ F+Q  T        ++RLR   F ++LRQ++ WFD  K  TG L T+L +D   ++     +LA   Q I++    II++   GW+L+ +    +PI+A    + +  +   ++K  K    +G IA E + + RTVV+ + ++K    Y+ +L        K+A   G+  S  Q +        ++   +  +  G   + +       V+ +F  IV G++A+G       ++  A  +A  + ++I++ P+I S+   G+ P    G+++F  V F YPTRP +P+LQ  +  +  G+T+A +G SG GKST++ LL+RFYDP+AG +   G ++K +++ W R+ +G+V QEP LF  SI ENI  G    +   EEIV++ +++N ++FI     K+NT +GD+G   SGGQKQRIAIA ALVR P +LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  755 FLILGIISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFETMYAQSLQIPYRNAMKKAHVFGITFSFTQAM--------MYFSYAACFRFGAYLVTQQLMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQGLKPNMLEGNVQFSGVVFNYPTRPSIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDK--AREGRTCIVIAHRLSTIQNAD 1237          
BLAST of SMED30003432 vs. Ensembl Mouse
Match: Abcb1b (ATP-binding cassette, sub-family B (MDR/TAP), member 1B [Source:MGI Symbol;Acc:MGI:97568])

HSP 1 Score: 438.343 bits (1126), Expect = 1.228e-139
Identity = 212/493 (43.00%), Postives = 319/493 (64.71%), Query Frame = -1
Query:    1 TFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1476
            +  E M      +  + +   I  +IQ+    L A RQ  ++R  +F+A++ QEI WFD    G+L T+LTDDV  I  GI DK+  F Q I++F+   I+ F +GWKL+ +     P++  + +L    +  ++ K+++AY+ AG +AEE+L++IRTV+AF  Q+KE++RY+ NL+EA+ +G+K++I   +  G+    ++   +L ++ G   +    Y  G V+ +F +I++G+ ++G+  P +E F  A+ AA+ +F++ID  P I S    G  P+S  G++EFKNV F YP+R  V IL+  N  +  G+TVA +G SG GKST + L+QR YDPL G +S  G D++ I++ + R  IGVV QEP LF+ +I ENI+ G+ D   +EI K+ +++NAY+FIMK   +F+T +G+RGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:  105 SLEEEMAIYAYYYTGIGAGVLIVAYIQVSLWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINDGIGDKIGMFFQSITTFLAGFIIGFISGWKLTLVILAVSPLIGLSSALWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQQKELERYNKNLEEAKNVGIKKAITASISIGIAYLLVYASYALAFWYGTSLVLSNEYSIGEVLTVFFSILLGTFSIGHLAPNIEAFANARGAAFEIFKIIDNEPSIDSFSTKGYKPDSIMGNLEFKNVHFNYPSRSEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDK--AREGRTTIVIAHRLSTVRNAD 595          

HSP 2 Score: 334.339 bits (856), Expect = 6.230e-101
Identity = 183/531 (34.46%), Postives = 299/531 (56.31%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNL--------KEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G   P+  ++F  +V +  S  D   T  +      + F ++   +F++ F Q  T        ++R+R   F ++LRQ+I WFD  K  TG L T+L  D  S++  +  +LA   Q +++    +IL+   GW+L+ +  + IP++     + +  +   ++K  K   I+G IA E + + RT+V+ + ++K    Y+ +L        K+A   G+  S  Q +        ++   +  +  G   + +       V+ +F  +V G++A GN      ++  AK +A  + ++I++ P+I S+   G+ P    G+++F  V F YPTRP +P+LQ  +  +  G+T+A +G SG GKST++ LL+RFYDP+AG +   G ++K +++ W R+H+G+V QEP LF  SI ENI  G       +EEIV++ +++N ++FI     K+NT +GD+G   SGGQKQRIAIA ALVR P +LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  720 GCIQPVFAIVFSRIVGVF-SRDDDHETKRQNCNLFSLFFLVMGLISFVTYFFQGFTFGKAGEILTKRVRYMVFKSMLRQDISWFDDHKNSTGSLTTRLASDASSVKGAMGARLAVVTQNVANLGTGVILSLVYGWQLTLLLVVIIPLIVLGGIIEMKLLSGQALKDKKQLEISGKIATEAIENFRTIVSLTREQKFETMYAQSLQVPYRNAMKKAHVFGITFSFTQAM--------MYFSYAACFRFGAYLVAQQLMTFENVMLVFSAVVFGAMAAGNTSSFAPDYAKAKVSASHIIRIIEKTPEIDSYSTEGLKPTLLEGNVKFNGVQFNYPTRPNIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRAVSHEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDK--AREGRTCIVIAHRLSTIQNAD 1239          
BLAST of SMED30003432 vs. Ensembl Mouse
Match: Abcb4 (ATP-binding cassette, sub-family B (MDR/TAP), member 4 [Source:MGI Symbol;Acc:MGI:97569])

HSP 1 Score: 417.927 bits (1073), Expect = 1.822e-135
Identity = 218/533 (40.90%), Postives = 330/533 (61.91%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVS-------PRDLTMT-------FSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G+  PL +++F ++ +  V        P + +++         E M      ++ L     ++ +IQ+   +L A RQ +++R  +F+A+LRQE+ WFD + T +L T+LTDDV  I  GI DK+  F Q I++F    I+ F  GWKL+ +     PI+  + ++    +  +S K++ AY+ AG +AEE L +IRTV+AF  Q KE++RY  +L+ A+ +G+K++I   +  G+    I+   +L ++ G   +    Y  G  + +F +I+IG+ ++G A P ++ F  A+ AAY +F +ID  P+I S  + G  P++  G++EF +V F YP+R  + IL+  N  +  G+TVA +G SG GKST + LLQR YDP  G+IS  G D+++ ++   R  IGVV QEP LFS +I ENI+ G+ +   +EI K+ +++NAY+FIMK  QKF+T +GDRGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE  VQ  LDK  A   RT +++AHRL T+RNAD
Sbjct:   65 GSGLPLMMIVFGEMTDKFVDNTGNFSLPVNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLVAAYIQVSFWTLAAGRQIKKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDVSKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDNIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGKISIDGQDIRNFNVRCLREIIGVVSQEPVLFSTTIAENIRYGRGNVTMDEIEKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDK--AREGRTTIVIAHRLSTIRNAD 595          

HSP 2 Score: 64.3142 bits (155), Expect = 3.623e-10
Identity = 36/111 (32.43%), Postives = 56/111 (50.45%), Query Frame = -1
Query: 1114 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMA 1440
            F  L   +F + F+Q  T        + RLRS  F A+LRQ++ WFD  K  TG L T+L  D   ++     +LA   Q  ++    II++F  GW+L+ +    +P +A
Sbjct:  755 FLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIA 865          
BLAST of SMED30003432 vs. Ensembl Mouse
Match: Abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:MGI Symbol;Acc:MGI:1924956])

HSP 1 Score: 418.313 bits (1074), Expect = 3.510e-132
Identity = 225/559 (40.25%), Postives = 346/559 (61.90%), Query Frame = -1
Query:    1 QLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSP----------RDLTMTFSERMRPSVIKFAI----LSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMG--LLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISH-KKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1626
            ++FRFA  +D++++ + I++S+  GA  PL  L+  ++ + L++           ++ + T  E++   +I   +    + +   I G++Q+    +TAARQ+ R+R  +F+++L Q+I WFD     +L T++T D+  +  GI DK+    Q IS F   ++++    WKLS +   + P++  + +L    I   + K++ AYS AG +AEE LSSI+TV AF AQEKE++RY+ +LK+A+  G+KR+    L  G + F +     L ++ G  L+F  E  Y  GT++ +F +++  S  +G+  P LE F  A+ AA+ +FQVID+ P I +    G +PE   G+IEFKNV F YP+RP   +L+  N  +  G+TVA +GPSGSGKST + LLQR YDP  G I+    D++  ++  YR  IGVV+QEP LF  +I  NI+ G+     +E+ ++ +++NAY+FIM   +KFNT +G++GA  SGGQKQRIAIA ALVRNP++L+LDEATSALD  SE LVQ  L+K  A   RT ++VAHRL T+R AD
Sbjct:   36 EIFRFADNLDIVLMTLGILASMINGATVPLMSLVLGEISDHLINGCLVQTNRTKYQNCSQT-QEKLNEDIIVLTLYYIGIGAAALIFGYVQISFWVITAARQTTRIRKQFFHSILAQDISWFDGSDICELNTRMTGDINKLCDGIGDKIPLMFQNISGFSIGLVISLIKSWKLSLVVLSTSPLIMASSALCSRMIISLTSKELDAYSKAGAVAEEALSSIQTVTAFGAQEKEIQRYTQHLKDAKDAGIKRATASKLSLGAVYFFMNGAYGLAFWYGTSLIFGGEPGYTIGTILAVFFSVIHSSYCIGSVAPHLETFTVARGAAFNIFQVIDKKPNIDNFSTAGFVPECIEGNIEFKNVSFSYPSRPSAKVLKGLNLKIKAGETVALVGPSGSGKSTTVQLLQRLYDPEDGCITVDENDIRAQNVRHYREQIGVVRQEPVLFGTTIGNNIKFGREGVGEKEMEQAAREANAYDFIMAFPKKFNTLVGEKGAQMSGGQKQRIAIARALVRNPKILILDEATSALDTESESLVQTALEK--ASKGRTTIVVAHRLSTIRGAD 591          

HSP 2 Score: 318.546 bits (815), Expect = 2.221e-95
Identity = 183/523 (34.99%), Postives = 286/523 (54.68%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQ--TGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAIN--EEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G+  P+  +IF  +V +       T+     +   ++   +L     ++  +Q +         + RLR + F A+L Q++ W+D ++  TG L T L  DV  I+   + +L    Q +S+    I+++F  GW+++ +     P++A T  +    +  ++ +  +A   AG IA E + +IRTVV+ + +    + Y   L+      +KR+   G    V    +    +  +  G   I  G   P  +  +F  I  G++A+G  +     +  AKA A  LF ++   P I S  + G  P++  G++EF+ V F YP RP VP+LQN + ++  GKTVAF+G SG GKST + LLQRFYDP+ G++   G D+K++++ W RS   +V QEP LF+ SI ENI  G    +   EEI +    +N + FI    +K+NT +G RG   SGGQKQR+AIA AL+R P++LLLDEATSALDN SEK+VQ+ LDK  A   +T ++VAHRL T++NAD
Sbjct:  704 GSVHPVFSIIFGKLVTMFEDKNKATLKQDAELYSMML--VVLGIVALVTYLMQGLFYGRAEENLAMRLRHSAFKAMLYQDMAWYDDKENNTGALTTTLAVDVAQIQGAATSRLGIVTQDVSNMSLSILISFIYGWEMTLLILSFAPVLAVTGMIQTAAMAGFANRDKQALKRAGKIATEAVENIRTVVSLTRERAFEQMYEETLQTQHRNALKRAHITGCCYAVSHAFVHFAHAAGFRFGAYLIQAGRMMPEGMFIVFTAIAYGAMAIGETLVWAPEYSKAKAGASHLFALLKNKPTINSCSQSGEKPDTCEGNLEFREVSFVYPCRPEVPVLQNMSLSIEKGKTVAFVGSSGCGKSTCVQLLQRFYDPMKGQVLLDGVDVKELNVQWLRSQTAIVSQEPVLFNCSIAENIAYGDNSRMVPLEEIKEVADAANIHSFIEGLPRKYNTLVGLRGVQLSGGQKQRLAIARALLRKPKILLLDEATSALDNESEKVVQQALDK--ARRGKTCLVVAHRLSTIQNAD 1222          
BLAST of SMED30003432 vs. Ensembl Mouse
Match: Abcb4 (ATP-binding cassette, sub-family B (MDR/TAP), member 4 [Source:MGI Symbol;Acc:MGI:97569])

HSP 1 Score: 417.157 bits (1071), Expect = 1.144e-131
Identity = 218/533 (40.90%), Postives = 330/533 (61.91%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVS-------PRDLTMT-------FSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G+  PL +++F ++ +  V        P + +++         E M      ++ L     ++ +IQ+   +L A RQ +++R  +F+A+LRQE+ WFD + T +L T+LTDDV  I  GI DK+  F Q I++F    I+ F  GWKL+ +     PI+  + ++    +  +S K++ AY+ AG +AEE L +IRTV+AF  Q KE++RY  +L+ A+ +G+K++I   +  G+    I+   +L ++ G   +    Y  G  + +F +I+IG+ ++G A P ++ F  A+ AAY +F +ID  P+I S  + G  P++  G++EF +V F YP+R  + IL+  N  +  G+TVA +G SG GKST + LLQR YDP  G+IS  G D+++ ++   R  IGVV QEP LFS +I ENI+ G+ +   +EI K+ +++NAY+FIMK  QKF+T +GDRGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE  VQ  LDK  A   RT +++AHRL T+RNAD
Sbjct:   65 GSGLPLMMIVFGEMTDKFVDNTGNFSLPVNFSLSMLNPGRILEEEMTRYAYYYSGLGGGVLVAAYIQVSFWTLAAGRQIKKIRQKFFHAILRQEMGWFDIKGTTELNTRLTDDVSKISEGIGDKVGMFFQAIATFFAGFIVGFIRGWKLTLVIMAISPILGLSTAVWAKILSTFSDKELAAYAKAGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKKIGIKKAISANISMGIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAAPCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDNIKGNLEFSDVHFSYPSRANIKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGKISIDGQDIRNFNVRCLREIIGVVSQEPVLFSTTIAENIRYGRGNVTMDEIEKAVKEANAYDFIMKLPQKFDTLVGDRGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAEVQAALDK--AREGRTTIVIAHRLSTIRNAD 595          

HSP 2 Score: 331.257 bits (848), Expect = 8.495e-100
Identity = 188/493 (38.13%), Postives = 280/493 (56.80%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQ--------EKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F  L   +F + F+Q  T        + RLRS  F A+LRQ++ WFD  K  TG L T+L  D   ++     +LA   Q  ++    II++F  GW+L+ +    +P +A    + +  +   + +  K    AG IA E + +IRTVV+ + +        EK    Y +++++A   G+  SI Q        F  F+     +  G   I  G      VI +F  IV+G++ALG+A     ++  AK +A  LF + +R P I S+   G+ P+ F G + F  V F YPTR  VP+LQ  +  +  G+T+A +G SG GKST++ LL+RFYDP+AG +   G + K +++ W R+ +G+V QEP LF  SI ENI  G    +  ++EIV++ +++N + FI    QK+NT +GD+G   SGGQKQRIAIA AL+R P++LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  755 FLGLGVLSFFTFFLQGFTFGKAGEILTTRLRSMAFKAMLRQDMSWFDDHKNSTGALSTRLATDAAQVQGATGTRLALIAQNTANLGTGIIISFIYGWQLTLLLLSVVPFIAVAGIVEMKMLAGNAKRDKKEMEAAGKIATEAIENIRTVVSLTQERKFESMYVEKLHGPYRNSVRKAHIYGITFSISQA-------FMYFSYAG-CFRFGSYLIVNGHMRFKDVILVFSAIVLGAVALGHASSFAPDYAKAKLSAAYLFSLFERQPLIDSYSGEGLWPDKFEGSVTFNEVVFNYPTRANVPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVLLDGQEAKKLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVPHDEIVRAAKEANIHPFIETLPQKYNTRVGDKGTQLSGGQKQRIAIARALIRQPRVLLLDEATSALDTESEKVVQEALDK--AREGRTCIVIAHRLSTIQNAD 1237          
BLAST of SMED30003432 vs. UniProt/SwissProt
Match: sp|P21447|MDR1A_MOUSE (ATP-dependent translocase ABCB1 OS=Mus musculus OX=10090 GN=Abcb1a PE=1 SV=3)

HSP 1 Score: 450.284 bits (1157), Expect = 3.332e-143
Identity = 234/562 (41.64%), Postives = 348/562 (61.92%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVS---------------PRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS   +FR+A  +D L ++V  +++I  G A PL +LIF D+ +   S                R +     E M      +  + +   I  +IQ+    L A RQ  ++R  +F+A++ QEI WFD    G+L T+LTDDV  I  GI DK+  F Q +++F G  I+ FT GWKL+ +     P++  +  +    +  ++ K++ AY+ AG +AEE+L++IRTV+AF  Q+KE++RY++NL+EA+ LG+K++I   +  G     I+   +L ++ G   +    Y  G V+ +F +++IG+ ++G A P +E F  A+ AAY +F++ID  P I S  K G  P++  G++EFKN+ F YP+R  V IL+  N  +  G+TVA +G SG GKST + L+QR YDPL G +S  G D++ I++ + R  IGVV QEP LF+ +I ENI+ G+ D   +EI K+ +++NAY+FIMK   +F+T +G+RGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:   33 VSVLTMFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLIFGDMTDSFASVGNVSKNSTNMSEADKRAMFAKLEEEMTTYAYYYTGIGAGVLIVAYIQVSFWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELHAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFSVLIGAFSVGQASPNIEAFANARGAAYEVFKIIDNKPSIDSFSKSGHKPDNIQGNLEFKNIHFSYPSRKEVQILKGLNLKVKSGQTVALVGNSGCGKSTTVQLMQRLYDPLDGMVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGREDVTMDEIEKAVKEANAYDFIMKLPHQFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDK--AREGRTTIVIAHRLSTVRNAD 592          

HSP 2 Score: 328.946 bits (842), Expect = 3.571e-98
Identity = 178/493 (36.11%), Postives = 282/493 (57.20%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNL--------KEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F IL   +FI+ F+Q  T        ++RLR   F ++LRQ++ WFD  K  TG L T+L +D   ++     +LA   Q I++    II++   GW+L+ +    +PI+A    + +  +   ++K  K    +G IA E + + RTVV+ + ++K    Y+ +L        K+A   G+  S  Q +        ++   +  +  G   + +       V+ +F  IV G++A+G       ++  A  +A  + ++I++ P+I S+   G+ P    G+++F  V F YPTRP +P+LQ  +  +  G+T+A +G SG GKST++ LL+RFYDP+AG +   G ++K +++ W R+ +G+V QEP LF  SI ENI  G    +   EEIV++ +++N ++FI     K+NT +GD+G   SGGQKQRIAIA ALVR P +LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  755 FLILGIISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGATGSRLAVIFQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFETMYAQSLQIPYRNAMKKAHVFGITFSFTQAM--------MYFSYAACFRFGAYLVTQQLMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKATVSASHIIRIIEKTPEIDSYSTQGLKPNMLEGNVQFSGVVFNYPTRPSIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGSVFLDGKEIKQLNVQWLRAQLGIVSQEPILFDCSIAENIAYGDNSRVVSYEEIVRAAKEANIHQFIDSLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDK--AREGRTCIVIAHRLSTIQNAD 1237          
BLAST of SMED30003432 vs. UniProt/SwissProt
Match: sp|P21448|MDR1_CRIGR (ATP-dependent translocase ABCB1 OS=Cricetulus griseus OX=10029 GN=ABCB1 PE=1 SV=2)

HSP 1 Score: 449.899 bits (1156), Expect = 3.439e-143
Identity = 233/563 (41.39%), Postives = 349/563 (61.99%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLL----------------VSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS + +FR+A  +D L ++V  +++I  G A PL +L+F D+ +                  V+  D+     E M      +  + +   I  +IQ+    L A RQ  ++R  +F+A++ QEI WFD    G+L T+LTDDV  I  GI DK+  F Q +++F G  I+ FT GWKL+ +     P++  +  +    +  ++ K+++AY+ AG +AEE+L++IRTV+AF  Q+KE++RY++NL+EA+ LG+K++I   +  G     I+   +L ++ G   +    Y  G V+ +F  ++IG+ ++G A P +E F  A+ AAY +F +ID  P I S  K+G  P++  G++EFKN+ F YP+R  V IL+  N  +  G+TVA +G SG GKST + LLQR YDP  G +S  G D++ I++ + R  IGVV QEP LF+ +I ENI+ G+ +   +EI K+ +++NAY+FIMK   KF+T +G+RGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:   33 VSVFTMFRYAGWLDRLYMLVGTLAAIIHGVALPLMMLVFGDMTDSFASVGNIPTNATNNATQVNASDIFGKLEEEMTTYAYYYTGIGAGVLIVAYIQVSFWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQAMATFFGGFIIGFTRGWKLTLVILAISPVLGLSAGIWAKILSSFTDKELQAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNNNLEEAKRLGIKKAITANISMGAAFLLIYASYALAFWYGTSLVISKEYSIGQVLTVFFAVLIGAFSIGQASPNIEAFANARGAAYEIFNIIDNKPSIDSFSKNGYKPDNIKGNLEFKNIHFSYPSRKDVQILKGLNLKVQSGQTVALVGNSGCGKSTTVQLLQRLYDPTEGVVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDK--AREGRTTIVIAHRLSTVRNAD 593          

HSP 2 Score: 331.643 bits (849), Expect = 3.553e-99
Identity = 188/531 (35.40%), Postives = 295/531 (55.56%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNL--------KEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA  P   +IF  VV +     D      +    S++ F IL   +FI+ F+Q  T        ++RLR   F ++LRQ++ WFD  K  TG L T+L +D   ++     +LA   Q I++    II++   GW+L+ +    +PI+A    + +  +   ++K  K    +G IA E + + RTVV+ + ++K    Y+ +L        K+A   G+  S  Q +        ++   +  +  G   +         V+ +F  IV G++A+G       ++  AK +A  +  +I++VP I S+   G+ P +  G+++F  V F YPTRP +P+LQ  N  +  G+T+A +G SG GKST++ LL+RFYDP+AG +   G ++  +++ W R+H+G+V QEP LF  SI ENI  G    +   +EI ++ +++N ++FI     K+NT +GD+G   SGGQKQRIAIA ALVR P +LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  719 GALQPAFSIIFSKVVGVFTRNTDDETKRHDSNLFSLL-FLILGVISFITFFLQGFTFGKAGEILTKRLRYMVFKSMLRQDVSWFDNPKNTTGALTTRLANDAGQVKGATGARLAVITQNIANLGTGIIISLIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTREQKFENMYAQSLQIPYRNALKKAHVFGITFSFTQAM--------MYFSYAACFRFGAYLVARELMTFENVLLVFSAIVFGAMAVGQVSSFAPDYAKAKVSASHIIMIIEKVPSIDSYSTGGLKPNTLEGNVKFNEVVFNYPTRPDIPVLQGLNLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPMAGTVFLDGKEVNQLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIERAAKEANIHQFIESLPDKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDK--AREGRTCIVIAHRLSTIQNAD 1238          
BLAST of SMED30003432 vs. UniProt/SwissProt
Match: sp|P08183|MDR1_HUMAN (ATP-dependent translocase ABCB1 OS=Homo sapiens OX=9606 GN=ABCB1 PE=1 SV=3)

HSP 1 Score: 449.899 bits (1156), Expect = 4.203e-143
Identity = 229/565 (40.53%), Postives = 354/565 (62.65%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLT------------------MTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS + +FR++  +D L ++V  +++I  GA  PL +L+F ++ ++  +  +L                   M   E M      ++ + +   ++ +IQ+    L A RQ  ++R  +F+A++RQEI WFD    G+L T+LTDDV  I  GI DK+  F Q +++F    I+ FT GWKL+ +     P++  + ++    +  ++ K++ AY+ AG +AEE+L++IRTV+AF  Q+KE++RY+ NL+EA+ +G+K++I   +  G     I+   +L ++ G   +  G Y  G V+ +F +++IG+ ++G A P +E F  A+ AAY +F++ID  P I S+ K G  P++  G++EF+NV F YP+R  V IL+  N  +  G+TVA +G SG GKST + L+QR YDP  G +S  G D++ I++ + R  IGVV QEP LF+ +I ENI+ G+ +   +EI K+ +++NAY+FIMK   KF+T +G+RGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:   34 VSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDK--ARKGRTTIVIAHRLSTVRNAD 596          

HSP 2 Score: 332.028 bits (850), Expect = 3.315e-99
Identity = 181/497 (36.42%), Postives = 286/497 (57.55%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGT------------VIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F  L   +FI+ F+Q  T        ++RLR   F ++LRQ++ WFD  K  TG L T+L +D   ++  I  +LA   Q I++    II++F  GW+L+ +    +PI+A    + +  +   ++K  K    +G IA E + + RTVV+ + ++K    Y+ +L+      ++++        + G T     +++Y     F Y G +  G             V+ +F  +V G++A+G       ++  AK +A  +  +I++ P I S+   G++P +  G++ F  V F YPTRP +P+LQ  +  +  G+T+A +G SG GKST++ LL+RFYDPLAG++   G ++K +++ W R+H+G+V QEP LF  SI ENI  G    +   EEIV++ +++N + FI     K++T +GD+G   SGGQKQRIAIA ALVR P +LLLDEATSALD  SEK+VQ+ LDK  A   RT +++AHRL T++NAD
Sbjct:  759 FLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKA-------HIFGITFSFTQAMMY-----FSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDK--AREGRTCIVIAHRLSTIQNAD 1241          
BLAST of SMED30003432 vs. UniProt/SwissProt
Match: sp|P43245|MDR1_RAT (ATP-dependent translocase ABCB1 OS=Rattus norvegicus OX=10116 GN=Abcb1 PE=2 SV=1)

HSP 1 Score: 439.884 bits (1130), Expect = 2.574e-139
Identity = 231/566 (40.81%), Postives = 348/566 (61.48%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRD--------------LTMTFS-----ERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V  + +FR+A  +D L + +  +++I  G   PL +L+F  + +     RD               T T S     E M      +  + +   I  +IQ+    L A RQ  ++R  +F+A++ QEI WFD    G+L T+LTDDV  I  GI DKL  F Q I++F    I+ F +GWKL+ +     P++  + ++    +  ++ K+++AY+ AG +AEE+L++IRTV+AF  Q+KE++RY+ NL+EA+ +G+K++I   +  G+    ++   +L ++ G   +    Y  G V+ +F +I++G+ ++G+  P +E F  A+ AAY +F++ID  P I S    G  P+S  G++EFKNV F YP+R  V IL+  N  +  G+TVA +G SG GKST + LLQR YDP+ GE+S  G D++ I++ + R  IGVV QEP LF+ +I ENI+ G+ +   +EI K+ +++NAY+FIMK   KF+T +G+RGA  SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:   32 VGIFGMFRYADWLDKLCMALGTLAAIIHGTLLPLLMLVFGYMTDSFTPSRDPHSDRAITNQSEINSTHTVSDTSLEEDMAMYAYYYTGIGAGVLIVAYIQVSLWCLAAGRQIHKIRQKFFHAIMNQEIGWFDVNDAGELNTRLTDDVSKINDGIGDKLGMFFQSITTFSAGFIIGFISGWKLTLVILAVSPLIGLSSAMWAKVLTSFTNKELQAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRVGIKKAITANISIGIAYLLVYASYALAFWYGTSLVLSNEYSIGQVLTVFFSILLGTFSIGHLAPNIEAFANARGAAYEIFKIIDNEPSIDSFSTKGHKPDSIMGNLEFKNVYFNYPSRSEVKILKGLNLKVKSGQTVALVGNSGCGKSTTVQLLQRLYDPIEGEVSIDGQDIRTINVRYLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQAALDK--AREGRTTIVIAHRLSTVRNAD 595          

HSP 2 Score: 319.316 bits (817), Expect = 1.306e-94
Identity = 181/525 (34.48%), Postives = 296/525 (56.38%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTT--GWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G   P+  ++F  +V +  S  D   T         + F ++   +F++ F Q  T        ++RLR   F ++LRQ+I WFD  K  TG L T+L  D  +++  +  +LA   Q +++    IIL+     GW+L+ +  + IP++     + +  +   ++K  K   I+G IA E + + RTVV+ + ++K    Y+ +L+      +K++   G+        I+   +  +  G   +         V+ +F  +V G++A GN      ++  AK +A  +  +I+++P+I S+   G+ P    G+++F  V F YPTRP +P+LQ  +F +  G+T+  +G SG GKST++ LL+RFY+P+AG +   G ++K +++   R+ +G+V QEP LF  SI ENI  G    +  +EEIV++ +++N ++FI    +K+NT +GD+G   SGGQKQRIAIA ALVR P +LLLDEATSALD  SEK+VQ+ LDK  A   RT V++AHRL T++NAD
Sbjct:  720 GCIQPVFAIVFSKIVGVF-SRDDDHETKQRNCNLFSLLFLVMGMISFVTYFFQGFTFGKAGEILTKRLRYMVFKSMLRQDISWFDDHKNTTGSLTTRLASDASNVKGAMGSRLAVVTQNVANLGTGIILSLVLVYGWQLTLLLVVIIPLIVLGGIIEMKLLSGQALKDKKELEISGKIATEAIENFRTVVSLTREQKFETMYAQSLQIPYRNALKKAHVFGITFAFTQAMIYFSYAACFRFGAYLVARELMTFENVMLVFSAVVFGAMAAGNTSSFAPDYAKAKVSASHIIGIIEKIPEIDSYSTEGLKPNWLEGNVKFNGVKFNYPTRPNIPVLQGLSFEVKKGQTLRLVGSSGCGKSTVVQLLERFYNPMAGTVFLDGKEIKQLNVQCVRA-LGIVSQEPILFDCSIAENIAYGDNSRVVSHEEIVRAAREANIHQFIDSLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDK--AREGRTCVVIAHRLSTIQNAD 1240          
BLAST of SMED30003432 vs. UniProt/SwissProt
Match: sp|Q2M3G0|ABCB5_HUMAN (ATP-binding cassette sub-family B member 5 OS=Homo sapiens OX=9606 GN=ABCB5 PE=1 SV=4)

HSP 1 Score: 439.113 bits (1128), Expect = 3.522e-139
Identity = 234/562 (41.64%), Postives = 354/562 (62.99%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSP----------RDLTMT---FSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGS--YDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISH-KKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V   ++FRFA G+D+ ++I+ I++S+  GA  PL  L+  ++ + L+S           ++ T +    +E M    + +  +     I G+IQ+    +TAARQ++R+R  +F+++L Q+I WFD    G+L T++TDD++ I  GI DK+A   Q +S+F   + +    GWKL+ +   + P++  + +     +   + K++ AYS AG +AEE+LSSIRTV+AF AQEKE++RY+ NLK+A+  G+KR+I   +  G + F +     L ++ G   I  G   Y  GTV+ +F +++  S  +G AVP  E F  A+ AA+ +FQVID+ P I +    G  PES  G +EFKNV F YP+RP + IL+  N  +  G+TVA +G +GSGKST++ LLQR YDP  G I     D++ +++  YR HIGVV QEP LF  +I  NI+ G+ D  +EE+ ++ +++NAY+FIM+   KFNT +G++GA  SGGQKQRIAIA ALVRNP++L+LDEATSALD+ S+  VQ  L+K  A   RT ++VAHRL T+R+AD
Sbjct:   31 VGSIEIFRFADGLDITLMILGILASLVNGACLPLMPLVLGEMSDNLISGCLVQTNTTNYQNCTQSQEKLNEDMTLLTLYYVGIGVAALIFGYIQISLWIITAARQTKRIRKQFFHSVLAQDIGWFDSCDIGELNTRMTDDIDKISDGIGDKIALLFQNMSTFSIGLAVGLVKGWKLTLVTLSTSPLIMASAAACSRMVISLTSKELSAYSKAGAVAEEVLSSIRTVIAFRAQEKELQRYTQNLKDAKDFGIKRTIASKVSLGAVYFFMNGTYGLAFWYGTSLILNGEPGYTIGTVLAVFFSVIHSSYCIGAAVPHFETFAIARGAAFHIFQVIDKKPSIDNFSTAGYKPESIEGTVEFKNVSFNYPSRPSIKILKGLNLRIKSGETVALVGLNGSGKSTVVQLLQRLYDPDDGFIMVDENDIRALNVRHYRDHIGVVSQEPVLFGTTISNNIKYGRDDVTDEEMERAAREANAYDFIMEFPNKFNTLVGEKGAQMSGGQKQRIAIARALVRNPKILILDEATSALDSESKSAVQAALEK--ASKGRTTIVVAHRLSTIRSAD 590          

HSP 2 Score: 310.457 bits (794), Expect = 1.479e-91
Identity = 185/531 (34.84%), Postives = 289/531 (54.43%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQ--TGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNL--------KEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAIN--EEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G   P+  +IF  ++ +  +    T+     +   +  F IL    F+S F+Q +         + RLR   F A+L Q+I WFD+++  TG L T L  D+  I+     ++    Q  ++    +I++F  GW+++F+     P++A T  +    +  ++ K  +    AG IA E L +IRT+V+ + ++   + Y   L        K+A+ +G     C       I F  +  G   +  G   I  G   P  +  +F  I  G++A+G  + +   +  AK+ A  LF ++++ P I S  + G  P++  G++EF+ V F YP RP V IL+  + ++  GKTVAF+G SG GKST + LLQR YDP+ G++ F G D K++++ W RS I +V QEP LF+ SI ENI  G    +   +EI ++   +N + FI    +K+NT +G +GA  SGGQKQR+AIA AL++ P++LLLDEATSALDN SEK+VQ  LDK  A   RT ++V HRL  ++NAD
Sbjct:  703 GTVHPVFSIIFAKIITMFGNNDKTTLKHDAEIYSMI--FVILGVICFVSYFMQGLFYGRAGEILTMRLRHLAFKAMLYQDIAWFDEKENSTGGLTTILAIDIAQIQGATGSRIGVLTQNATNMGLSVIISFIYGWEMTFLILSIAPVLAVTGMIETAAMTGFANKDKQELKHAGKIATEALENIRTIVSLTREKAFEQMYEEMLQTQHRNTSKKAQIIGS----CYAFSHAFIYFA-YAAG---FRFGAYLIQAGRMTPEGMFIVFTAIAYGAMAIGETLVLAPEYSKAKSGAAHLFALLEKKPNIDSRSQEGKKPDTCEGNLEFREVSFFYPCRPDVFILRGLSLSIERGKTVAFVGSSGCGKSTSVQLLQRLYDPVQGQVLFDGVDAKELNVQWLRSQIAIVPQEPVLFNCSIAENIAYGDNSRVVPLDEIKEAANAANIHSFIEGLPEKYNTQVGLKGAQLSGGQKQRLAIARALLQKPKILLLDEATSALDNDSEKVVQHALDK--ARTGRTCLVVTHRLSAIQNAD 1221          
BLAST of SMED30003432 vs. TrEMBL
Match: A0A4S2MG49 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_000267 PE=4 SV=1)

HSP 1 Score: 521.546 bits (1342), Expect = 2.538e-168
Identity = 258/550 (46.91%), Postives = 373/550 (67.82%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVS----PRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V +++LF++A   D   ++  ++ ++  GA FPLN+ I+R  ++  VS    P D+          +V  F IL +  F   FIQ + + ++++RQ RR+R  YF A+LRQ++ WF+KQ +G LV KL+++V+ I++GI  K   FIQ IS F+  +I+AF  GWKLS +    +P++A  F+L    +K  ++K++ AYS AGGIA E+LS+IRTVVAF  +EKE  RYS  L  A+  GVK+S+  G   G+IG T+FT  ++V++ G+  +    Y  GTV+ +F  +++GSI LGNA+P L+ F+TA AAA  ++  I+R+P I     G  PE F+G+I F++++F YPTRP   +LQ F+  L  G+TVA +GPSGSGKST++H+LQRFY+P+ G I   GTD++++DL  +RS  G VQQEP LF G++ ENI++GK+DA   EI ++ + +NA++FI+   + +NT +G+RG G SGGQKQRIAIA AL+R P+LLLLDEATSALD  SE++VQ  LDK  A   RTVVMVAHRL TVRNAD
Sbjct:   18 VKFWRLFQYADAADKACIVCSVLCALGAGAGFPLNIFIYRGTIDNFVSTEFSPTDVY--------ANVKWFVILGAVIFCITFIQSMLLGISSSRQGRRIRLLYFEAILRQDVPWFEKQTSGGLVQKLSENVDIIQNGIGTKFGDFIQNISGFLAGLIIAFAVGWKLSLVAFAMLPLVAIAFALFGFLMKILTMKEVSAYSRAGGIANEVLSAIRTVVAFGGEEKEYNRYSAELTTAQKQGVKKSMAVGGVMGLIGLTLFTSAAVVFWYGVELMLTAEYTAGTVVAVFFNVILGSIYLGNALPGLQYFLTATAAARDVYDTIERIPSIDKNYAGTFPEDFHGNINFQDIEFVYPTRPDTTVLQEFSMNLREGQTVALVGPSGSGKSTVVHMLQRFYEPVEGRILVEGTDIRELDLKAFRSQQGFVQQEPVLFEGTVAENIRMGKLDADQAEIEEAARLANAHDFILSLPEGYNTVVGERGTGMSGGQKQRIAIARALIRKPRLLLLDEATSALDTNSERIVQAALDK--ASSGRTVVMVAHRLTTVRNAD 557          

HSP 2 Score: 280.796 bits (717), Expect = 1.688e-78
Identity = 167/472 (35.38%), Postives = 263/472 (55.72%), Query Frame = -1
Query:    1 LTAARQSRRLRSNYFNALLRQEICWFDKQ--QTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSH----NLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLA----GEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1380
            ++  R ++RLRS  F A+L Q+I WFD+Q  Q G L  +L  +   +++     L   ++     +  I++AF   W+L+ +     P++  +  L +  ++      +  +++   IA+E LS+ +TV AF+ ++   KR+ +    NLK      +  S+   L   ++ F      SL  Y+    + + S     + ++F+ + + S +LG    ++     A  AA  +F  +DR+P I         E F G +EFKNV F YP+RPG  IL+ F+  +  G++VA +G SG GKST++ L+QRFYDP+       + F G +L+ +  +W R  IG+V QEP LF  SI+ENI  G    +   EEI+++ + +N ++F+    Q ++T +G RG   SGGQKQR+AIA AL+R P LLLLDEATSALDN SE++VQ+ LD  +  GK T ++VAHRL TV N D
Sbjct:  734 VSGERLTQRLRSQLFKAILSQDIGWFDRQENQPGILTARLATEASKLKALSGSSLGFIVEAGVLSIISIVVAFIYSWQLALLVLGFAPVLVLSGMLQVKRMQGGDGTSVSLFAMK--IAQEALSAEKTVFAFNLEDYFYKRFGNALQSNLKSELKDNLVTSLVFALTQSIMMFCFAASMSLGAYL----LNQNSLTLVGLFRVFIVLNMSSQSLGRTASIVPELTAAAKAAKSIFSTMDRIPHILTDAGEKPIEQFTGQVEFKNVTFTYPSRPGTRILKRFSHCISAGESVALVGVSGCGKSTLLQLVQRFYDPIHTGPDSGVFFDGHNLRSLAPSWIRRQIGIVSQEPNLFDLSIRENIAYGDNSKEVSMEEIIEAARQANIHDFVSTLPQGYDTQVGARGGKLSGGQKQRVAIARALIRKPALLLLDEATSALDNESERIVQQALDGIV--GKCTSIVVAHRLTTVENVD 1197          
BLAST of SMED30003432 vs. TrEMBL
Match: A0A4V6RH95 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_000267 PE=4 SV=1)

HSP 1 Score: 521.546 bits (1342), Expect = 3.713e-168
Identity = 258/550 (46.91%), Postives = 373/550 (67.82%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVS----PRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V +++LF++A   D   ++  ++ ++  GA FPLN+ I+R  ++  VS    P D+          +V  F IL +  F   FIQ + + ++++RQ RR+R  YF A+LRQ++ WF+KQ +G LV KL+++V+ I++GI  K   FIQ IS F+  +I+AF  GWKLS +    +P++A  F+L    +K  ++K++ AYS AGGIA E+LS+IRTVVAF  +EKE  RYS  L  A+  GVK+S+  G   G+IG T+FT  ++V++ G+  +    Y  GTV+ +F  +++GSI LGNA+P L+ F+TA AAA  ++  I+R+P I     G  PE F+G+I F++++F YPTRP   +LQ F+  L  G+TVA +GPSGSGKST++H+LQRFY+P+ G I   GTD++++DL  +RS  G VQQEP LF G++ ENI++GK+DA   EI ++ + +NA++FI+   + +NT +G+RG G SGGQKQRIAIA AL+R P+LLLLDEATSALD  SE++VQ  LDK  A   RTVVMVAHRL TVRNAD
Sbjct:   31 VKFWRLFQYADAADKACIVCSVLCALGAGAGFPLNIFIYRGTIDNFVSTEFSPTDVY--------ANVKWFVILGAVIFCITFIQSMLLGISSSRQGRRIRLLYFEAILRQDVPWFEKQTSGGLVQKLSENVDIIQNGIGTKFGDFIQNISGFLAGLIIAFAVGWKLSLVAFAMLPLVAIAFALFGFLMKILTMKEVSAYSRAGGIANEVLSAIRTVVAFGGEEKEYNRYSAELTTAQKQGVKKSMAVGGVMGLIGLTLFTSAAVVFWYGVELMLTAEYTAGTVVAVFFNVILGSIYLGNALPGLQYFLTATAAARDVYDTIERIPSIDKNYAGTFPEDFHGNINFQDIEFVYPTRPDTTVLQEFSMNLREGQTVALVGPSGSGKSTVVHMLQRFYEPVEGRILVEGTDIRELDLKAFRSQQGFVQQEPVLFEGTVAENIRMGKLDADQAEIEEAARLANAHDFILSLPEGYNTVVGERGTGMSGGQKQRIAIARALIRKPRLLLLDEATSALDTNSERIVQAALDK--ASSGRTVVMVAHRLTTVRNAD 570          

HSP 2 Score: 280.796 bits (717), Expect = 1.566e-78
Identity = 167/472 (35.38%), Postives = 263/472 (55.72%), Query Frame = -1
Query:    1 LTAARQSRRLRSNYFNALLRQEICWFDKQ--QTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSH----NLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLA----GEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1380
            ++  R ++RLRS  F A+L Q+I WFD+Q  Q G L  +L  +   +++     L   ++     +  I++AF   W+L+ +     P++  +  L +  ++      +  +++   IA+E LS+ +TV AF+ ++   KR+ +    NLK      +  S+   L   ++ F      SL  Y+    + + S     + ++F+ + + S +LG    ++     A  AA  +F  +DR+P I         E F G +EFKNV F YP+RPG  IL+ F+  +  G++VA +G SG GKST++ L+QRFYDP+       + F G +L+ +  +W R  IG+V QEP LF  SI+ENI  G    +   EEI+++ + +N ++F+    Q ++T +G RG   SGGQKQR+AIA AL+R P LLLLDEATSALDN SE++VQ+ LD  +  GK T ++VAHRL TV N D
Sbjct:  747 VSGERLTQRLRSQLFKAILSQDIGWFDRQENQPGILTARLATEASKLKALSGSSLGFIVEAGVLSIISIVVAFIYSWQLALLVLGFAPVLVLSGMLQVKRMQGGDGTSVSLFAMK--IAQEALSAEKTVFAFNLEDYFYKRFGNALQSNLKSELKDNLVTSLVFALTQSIMMFCFAASMSLGAYL----LNQNSLTLVGLFRVFIVLNMSSQSLGRTASIVPELTAAAKAAKSIFSTMDRIPHILTDAGEKPIEQFTGQVEFKNVTFTYPSRPGTRILKRFSHCISAGESVALVGVSGCGKSTLLQLVQRFYDPIHTGPDSGVFFDGHNLRSLAPSWIRRQIGIVSQEPNLFDLSIRENIAYGDNSKEVSMEEIIEAARQANIHDFVSTLPQGYDTQVGARGGKLSGGQKQRVAIARALIRKPALLLLDEATSALDNESERIVQQALDGIV--GKCTSIVVAHRLTTVENVD 1210          
BLAST of SMED30003432 vs. TrEMBL
Match: A0A4E0RLC9 (Multidrug resistance protein 1 OS=Fasciola hepatica OX=6192 GN=D915_007290 PE=4 SV=1)

HSP 1 Score: 513.072 bits (1320), Expect = 5.996e-165
Identity = 255/547 (46.62%), Postives = 372/547 (68.01%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIK-FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS+++LF  A   D + ++   I S+  GA FPLNVLIFR +++  + P     +F   +   ++K FA+L +C  +  F+Q     ++A RQSRR+R  YF A+LRQ++ WFD Q +G L+ KLT ++++IE GI  K   FI  +S F   +I+AF  GWKL+ +    +P + T F      +K +++K++ AYS A  I+ E+LS+IRTV+AF  ++KE +RYS  L  A+ +G+ +S   G   G IG T+F+  +L+++ G+  I   +Y  GTV+ +F+ +VIGSI LGNA+P ++ F+ A A+A  ++ +IDRVP I   + G     F G+I FK+VDF YPTRP + +L+ FN  L  G+TVA +GPSGSGKSTI+H+LQRFYDP+ GEI   G +++D+DL  +R+ +G VQQEP LF G++++NI+LGK+DA NEEI+++ + +NA++FI+   + + T L +RGAG SGGQKQRIAIA AL+R P+LLLLDEATSALD  SE++VQ+ LD+  A   RTVV+VAHRL TVRNAD
Sbjct:   22 VSFFKLFSHADLRDKMCIVFGFIFSVASGAGFPLNVLIFRSIIDQFIGP-----SFDASLIYGIVKWFAVLGACLIVLTFLQSFLFGISAQRQSRRIRVKYFKAVLRQDVPWFDTQSSGSLMNKLTQNIDNIEEGIGSKFGEFILNLSGFFCGLIIAFFVGWKLTLVACAMVPFVVTVFLFFGVSMKYFTMKELDAYSRASAISGEVLSAIRTVMAFGGEKKEFQRYSKELGAAQKMGIIKSAALGGVVGAIGLTLFSSAALIFWYGIELIITENYSGGTVVTVFINVVIGSIFLGNALPNMQYFLIAMASATEVYGLIDRVPPIDKDRSGKRIPDFTGNIIFKDVDFAYPTRPDIVVLKKFNLELKSGQTVALVGPSGSGKSTIVHMLQRFYDPIGGEIIVEGENIRDLDLKAFRAQLGCVQQEPILFEGTVEDNIRLGKLDATNEEIIEAAKMANAHDFILHLPEGYKTVLAERGAGLSGGQKQRIAIARALIRKPKLLLLDEATSALDTRSERVVQEALDQ--ASAGRTVVVVAHRLTTVRNAD 561          

HSP 2 Score: 252.677 bits (644), Expect = 1.126e-68
Identity = 183/540 (33.89%), Postives = 278/540 (51.48%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFA----ILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDK--QQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSH--------NLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSY-DPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIP-ESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDP----LAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA  P   L++  +  +     D      E+MR  V  FA     L    F+    Q     ++  R +RR+R   F A++RQEI WFD+   Q G L   L  +   ++S    +L   I+  +     +++AF   W+L+ +     P+   +  L I  I     K+    S    +A+E +SS RTV   + ++    R+          NLK+     V  S+ Q  G        FT+G+        F+ E  Y +   V ++F  + + + +LG    +      A+ A+  + + +DR P I     G++P E F G++EFK+V F YPTR  + +L+ F+  +  G+TVA +G SG GKST++ L+QRFYDP        I F G +++ +   W R  IG+V QEP LF  SI++NI  G    +   +EI+++ + +N +EFI    + ++T +G +G+  SGGQKQR+AIA AL+R P LLLLDEATSALD  SE++VQ+ LD   A G RT +MVAHRL TV NAD
Sbjct:  694 GAVQPSFALLYSQMFQIFTYAND-----PEKMRSQVSIFAALMVFLGCLRFLGMLGQGFFFGVSGERLTRRVRGMLFQAIMRQEIGWFDRTENQPGTLTAVLATEASKMKSLSGSQLGFIIEAFALVTMSLVIAFIYSWQLTLLMLAFYPMFILSGMLQIKRIAGEGSKETDGPSTQ--LAQEAISSDRTVFTLNLEDYFQNRFEDLLMNKKLSNLKQTILFAVLFSLTQA-GPMFCFAAAFTLGA--------FLVEHKYIEMLAVFRVFAVLNMSAQSLGRTASIGPETQRAREASKIILKTLDRTPSI-RTDDGLVPREPFRGNVEFKHVYFSYPTRKTISVLKKFSHAVNSGETVALVGESGCGKSTLLQLVQRFYDPDDFGSDSGIFFDGHNIRQLAPAWVRRQIGIVSQEPNLFDMSIRDNIAYGDNSREVTMDEIMEAARQANVHEFITTLPEGYDTSVGQKGSHLSGGQKQRVAIARALLRKPILLLLDEATSALDVESERVVQEALDS--AMGSRTCLMVAHRLSTVENAD 1214          
BLAST of SMED30003432 vs. TrEMBL
Match: A0A504YDC3 (Multidrug resistance protein 1 OS=Fasciola gigantica OX=46835 GN=FGIG_06363 PE=4 SV=1)

HSP 1 Score: 506.908 bits (1304), Expect = 1.476e-162
Identity = 253/547 (46.25%), Postives = 371/547 (67.82%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIK-FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS+++LF  A   D + +++  I S+  GA FPLNVLIFR +++  + P     +F   +   ++K FA+L +C  +  F Q     ++A RQSRR+R  YF A+LRQ++ WFD Q +G L+ KLT ++++IE GI  K   FI  +S F   +I+AF  GWKL+ +    +P + T F      +K +++K++ AYS A  I+ E+LS+IRTV+A+  ++KE +RYS  L  A+ +G+ +S   G   G IG T+F+  +L+++ G+  I   +Y  GTV+ +F+ ++IGSI LGNA+P ++ F+ A A+A  ++ +IDRVP I   + G     F G+I FK+VDF YPTR  + +L+ FN  L  G+TVA +GPSGSGKSTIIH+LQRFYDP+ GEI   G +++D+DL  +R+ +G VQQEP LF G++++NI+LGK+DA NEEI+++ + +NA++FI+   + + T L +RGAG SGGQKQRIAIA AL+R P+LLLLDEATSALD  SE++VQ+ LD+  A   RTVV+VAHRL TVRNAD
Sbjct:   22 VSFFKLFSHADLHDKMCIVLGSIFSVASGAGFPLNVLIFRSIIDQFIGP-----SFDASLIYGIVKWFAVLGACLIVLTFSQSFLFGISAQRQSRRIRVKYFKAILRQDVPWFDTQSSGSLMNKLTQNIDNIEEGIGSKFGEFILNLSGFFCGLIIAFFVGWKLTLVACAMVPFVVTVFLFFGVSMKYFTMKELDAYSRASAISGEVLSAIRTVMAYGGEKKEFQRYSKELGAAQKMGIIKSAALGGVVGAIGLTLFSSAALIFWYGIELIITENYSGGTVVTVFINVIIGSIFLGNALPNMQYFLIAMASATEVYSLIDRVPPIDKDQSGKGIPDFTGNIIFKDVDFAYPTRSDIVVLKKFNLELKSGQTVALVGPSGSGKSTIIHMLQRFYDPIGGEIIVEGENIRDLDLKAFRAQLGCVQQEPVLFEGTVEDNIRLGKLDATNEEIIEAAKMANAHDFILHLPEGYKTVLAERGAGLSGGQKQRIAIARALIRKPKLLLLDEATSALDTRSERVVQEALDQ--ASTGRTVVVVAHRLTTVRNAD 561          

HSP 2 Score: 255.373 bits (651), Expect = 1.490e-69
Identity = 185/540 (34.26%), Postives = 279/540 (51.67%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAIL----SSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDK--QQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSH--------NLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSY-DPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIP-ESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDP----LAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA  P   L++  +  +     D      E+MR  V  FA+L        F+    Q     ++  R +RR+R   F A++RQEI WFD+   Q G L   L  +   ++S    +L   I+  +     +++AF   W+L+ +     P+   +  L I  I     K+    S    +A+E +SS RTV   + ++    R+          NLK+     V  S+ Q  G        FT+G+        F+ E  Y +   V ++F  + + + +LG    +      A+ A+  + + +DR P I     G++P E F G++EFK+V F YPTR  + +L+ F+  +  G+TVA +G SG GKST++ L+QRFYDP        I F G +++ +   W R  IGVV QEP LF  SI++NI  G    +   +EI+++ + +N +EFI    + ++T +G +G+  SGGQKQR+AIA AL+R P LLLLDEATSALD  SE++VQK LD   A G RT +MVAHRL TV NAD
Sbjct:  694 GAVQPSFALLYSQMFQIFTYAND-----PEKMRSQVSIFAVLMVFLGCLRFLGMLGQGFFFGVSGERLTRRVRGMLFQAIMRQEIGWFDRTENQPGTLTAVLATEASKMKSLSGSQLGFIIEAFALVTMSLVIAFIYSWQLTLLMLAFYPMFILSGMLQIKRIAGEESKETDGPSTQ--LAQEAISSDRTVFTLNLEDYFQNRFEDLLMNKKLSNLKQTILFAVLFSLTQA-GPMFCFAAAFTLGA--------FLVEHKYIEMLAVFRVFAVLNMSAQSLGRTASIGPETQRAREASKIILETMDRTPSI-RTDDGLVPREPFRGNVEFKHVYFNYPTRKTISVLKKFSHAVNSGETVALVGESGCGKSTLLQLVQRFYDPDDFGSDSGIFFDGHNIRQLAPAWVRRQIGVVSQEPNLFDMSIRDNIAYGDNSREVTMDEIMEAARQANIHEFITTLPEGYDTSVGQKGSRLSGGQKQRVAIARALLRKPILLLLDEATSALDVESEQVVQKALDS--AMGSRTCLMVAHRLSTVENAD 1214          
BLAST of SMED30003432 vs. TrEMBL
Match: A0A4E0RE86 (ATP binding cassette subfamily B MDR TAP OS=Fasciola hepatica OX=6192 GN=D915_004337 PE=4 SV=1)

HSP 1 Score: 506.908 bits (1304), Expect = 1.558e-162
Identity = 250/548 (45.62%), Postives = 371/548 (67.70%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLTMT-FSERMRPSVIK-FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            +S+++LF  A   D   +    I +I  G  FPLN+LIFR ++N      + T T F+  +   ++  FAIL +C  +  F+Q    + +A RQS+R+R  YF A++RQ++ WFD+Q TG L++KLT ++++IE GI  K   FI  IS F   II+AF  GWKL+ +    IP++   F+L    +KK+++K+++AY+ A GI+ E+ S+IRTVVAF  ++KE++RYS  L  A+ +G+++S   G   G IG  +F   +LV++ G+  + +  Y  G+++ +F+ I++GSI LGNA+P ++ F+ A ++A  ++  IDRVP I   K G     F G+I FK+VDF YP R    +L+ FN  L  G+TVA +GPSGSGKST++H+LQRFYDP+ GEI   G +++D+DL  +R+ +G VQQEP LF G++++NI+LGK+DA NEEI+++ + +NA++FI+   + + T L +RGAG SGGQKQRIAIA AL+R P+LLLLDEATSALD  SE++VQ+ LD+  A   RTVV+VAHRL TVRNAD
Sbjct:   22 ISFFKLFSHADARDKACIFFGSIFAIASGTGFPLNILIFRSIIN------EFTGTNFAASVIYDIVGWFAILGACLAVVSFLQSFLFATSAERQSKRIRLKYFKAIMRQDVPWFDEQSTGALMSKLTQNIDNIEEGIGTKFGEFILNISGFFCGIIIAFAVGWKLALVACCMIPLVVIVFALFGFLMKKFTLKELQAYAQASGISGEVFSAIRTVVAFGGEKKELQRYSKELGAAQKVGIQKSTALGGVMGAIGLALFCAAALVFWYGIELLLKEGYRGGSIVTVFVNIIVGSIFLGNALPSMQYFLIAMSSAAEVYGTIDRVPPIDKNKSGKHIPDFAGNIIFKDVDFAYPLRSDAIVLKKFNLELKSGQTVALVGPSGSGKSTVVHMLQRFYDPIGGEIIVEGENIRDLDLKAFRAQLGCVQQEPILFEGTVEDNIRLGKLDATNEEIIEAAKMANAHDFILHLPEGYKTVLAERGAGLSGGQKQRIAIARALIRKPKLLLLDEATSALDTRSERVVQEALDQ--ASAGRTVVVVAHRLTTVRNAD 561          

HSP 2 Score: 246.128 bits (627), Expect = 2.101e-66
Identity = 167/506 (33.00%), Postives = 267/506 (52.77%), Query Frame = -1
Query:    1 ERMRPSVIKFA----ILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDK--QQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSL-----VYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLA----GEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1467
            ++MR  V  FA    ++    F+S   Q     ++  R +RR+R   F A+++QEI WFD+   Q G L   L  +   ++S    +L   ++ I   V  +++ F   W+L+ +     P+   T  + I  +   + K+    S    +A+E + S RT+   + ++    RY ++L     L  K+S  + +    + F++   G +      + +G   +         V ++F    + + +LG         + A+ A+  + Q++DR P I   +  V  E F G +EFK + F YPTR  + +L+NF+  +  G+TVA +G SG GKST++ L+QRFYDP++      I F G +++ +   W R  IG+V QEP LF  SI++NI  G    +   +EI+++ + +N +EFI    + + T  G +G+  SGGQKQR+AIA AL+R P LLLLDEATSALD  SE++VQ  LD   A G RT ++VAHRL TV NAD
Sbjct:  721 DKMRSQVGLFAGLMVLMGVLRFLSMLGQGFFFGVSGERLTRRVRCKLFEAVMKQEIGWFDRTENQPGVLTVLLATEASKLKSISGAQLGYIVEAIVMVVMSLVITFIYSWQLTLLMLAFYPLFVLTGMIQIQRMSGGNSKKTDITSTR--VAQEAIGSDRTLFTLTLEDYFYSRYENSL-----LHNKKSNLKKIAVYAVLFSLTQAGPMFCFAAAFALGAYLVGRHDIQMLAVFRVFSVQNLTAQSLGRTASYGPESLRAQNASKAILQLLDRNPSIRTDEGSVPQEPFKGHVEFKRIYFSYPTRKTLVVLKNFSHVVNPGETVALVGESGCGKSTLLQLVQRFYDPISVGSDSGIFFDGHNIRQLAPAWVRRQIGIVSQEPNLFDMSIRDNIAYGDNSREVTIDEIIEAARQANIHEFITTLPEGYETSAGQKGSRLSGGQKQRVAIARALLRKPILLLLDEATSALDVESERVVQDALDS--AMGSRTCLVVAHRLSTVENAD 1217          
BLAST of SMED30003432 vs. Ensembl Cavefish
Match: abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6414])

HSP 1 Score: 439.884 bits (1130), Expect = 2.530e-140
Identity = 228/570 (40.00%), Postives = 349/570 (61.23%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVN-LLVSPRDLTMT----FSERMRPSVIK----------------FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEG--SYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDR-VPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V ++QLFR+AT +++L++++ +  +   G   P+  ++F  + +  ++S + L +T    FS  +    +                 F  L +   + G  Q+    LTA RQ++R+R  YF+A+L Q + WFD    G+L T+LTDD+ +I  G+ DK+  F+Q   +F+   I+ F  GWKL+ +     P++A + ++    +   + K++ AY+ AG +AEEIL +IRTVVAF+ Q+K  ++Y  NL  A+  GVK+++   +  G+  F IF   +L ++ G     +   +Y  G VI +F +++IG+ +LG   P LE+   A+ AAY ++  ID   P  S  + G  P+S  GDIEF+NV F YP+R  V ILQ  +  +P+GKT+A +G SG GKST I LLQRFYDP AGE++  G D++ +++ W R ++G+V QEP LF  +I ENI+ G+ DA +++I ++ +++NAYEFI K     NT +G+RGA  SGGQKQRIAIA ALV+NP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL T+R AD
Sbjct:    4 VGFFQLFRYATCLEVLMMVLGLGCAALHGITLPIMCVVFGQMTDSFVLSGQQLNLTGLGNFSTNLSSGCVAIPGIDIEASMSQHAYYFVALGAAVLLLGTFQVTLFLLTATRQTKRIREKYFHAVLHQPMAWFDTHPIGELNTRLTDDINTINDGLGDKICIFVQFFCTFLAGFIIGFIYGWKLTLVIMSVSPLLAASAAVWSKILATLTSKELSAYAKAGAVAEEILVAIRTVVAFNGQQKAAEKYEKNLITAKDFGVKKAVTTNVSMGLTQFFIFGAYALAFWYGTKLTVDDPVNYSIGKVIIVFFSVMIGAFSLGQGAPNLESVAKARGAAYSIYNTIDMPRPIDSSSEEGYKPDSVRGDIEFRNVHFSYPSRKDVKILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPEAGEVTLDGRDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDATDQDIDRAVREANAYEFISKLPDGLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSEAIVQAALDK--ARAGRTTIVIAHRLSTIRTAD 571          

HSP 2 Score: 318.546 bits (815), Expect = 2.271e-95
Identity = 182/528 (34.47%), Postives = 290/528 (54.92%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSP-----RDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G  +P   ++F  ++ +   P     R   + FS       + F ++ +  F++ F Q      +    + RLRS  F A+LRQ+I WFD      G L TKL  D   ++     +L      I + V  +I+AF   W+L+ +     PI+     + +  +  ++ K   A  ++G I+ E + + +TVV+ + ++   ++++ +L +     + ++   G+   +     + V + V+  G   I         V  +F  IV  ++++G +     +F  AKAAA  +  ++++ P+I  + + G  P +F GDIEF++V F YPTRP + ILQ  N ++  G+T+A +G SG GKST I LL+RFY+P  G++   G D + ++L W RS +G+V QEP LF  +I ENIQ G    +   EE+V++   +N + FI    +K+NT +GD+G   SGGQKQRIAIA ALVR P++LLLDEATSALD  SEK+VQK LD+  A   RT +++AHRL TV+NAD
Sbjct:  714 GGVYPAVAILFAKIIGVFSEPDPEVKRQKALMFS-------LLFLLVGAVAFLTFFFQGYMFGKSGEILTMRLRSQAFKAILRQDISWFDDHNNAVGVLTTKLATDASLVKGAAGSRLGLATNTICALVIAMIVAFFHSWQLTLMILACTPILTGANFIQMRAMAGHTSKDQSALEMSGRISTETVENFKTVVSLTKEDVFFRKFNDSLNKPYRSALCKAPIYGITFALAQAIPYFVNAAVFRFGSWLIAHCHTQFENVFLVFSVIVFAAMSIGQSSSFAPDFAKAKAAAGRILALLEKTPEIDIYSEAGDKPINFKGDIEFRDVHFFYPTRPNIKILQGLNVSVTQGQTLALVGGSGCGKSTSIQLLERFYNPAGGQVFADGMDTRGLNLAWLRSQLGLVSQEPILFDCTITENIQYGDNSRVVSQEEVVEAAISANIHNFISDLPEKYNTRVGDKGTQLSGGQKQRIAIARALVRKPKVLLLDEATSALDTESEKIVQKALDE--ARKGRTCIVIAHRLTTVQNAD 1232          
BLAST of SMED30003432 vs. Ensembl Cavefish
Match: abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6414])

HSP 1 Score: 422.935 bits (1086), Expect = 8.789e-135
Identity = 210/483 (43.48%), Postives = 310/483 (64.18%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEG--SYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDR-VPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F  + +   + G  Q++   LTA RQ++R+R  YF+A+L Q++ WFD    G+L T+LTDD+ +I  G+ DK+  F+Q    F+  II+ F  GWKL+ +     P++A + ++    +   + K++ AY+ AG +AEEIL +IRTVVAF+ Q+K +++Y  NL++A+  GVK++I   +  G+  F IF   +L ++ G     +   +Y  G VI +F +++IG+ +LG   P LE+   A+ AAY ++  ID   P  S  K G  P+S  GDIEFKN+ F YP+R  V ILQ  +  +P+GKT+A +G SG GKST I LLQRFYDP AGE++  G D++ +++ W R ++G+V QEP LF  +I ENI+ G+ DA +++I ++ +++NAYEFI K     NT +G+RGA  SGGQKQRIAIA ALV+NP++LLLDEATSALD  SE   Q       A   RT +++AHRL T+R AD
Sbjct:   25 FVGIGAGVLLLGTFQVMLFLLTATRQTKRIREKYFHAVLHQQMAWFDTHPIGELNTRLTDDINTINDGLGDKICIFVQFFCRFLAGIIIGFIYGWKLTLVIMSVSPLLAGSAAVWSKILATLTGKELSAYAKAGAVAEEILVAIRTVVAFNGQQKALEKYEKNLEDAKNFGVKKAITTNVSMGITQFFIFGTYALAFWYGTKLSVDEPENYSIGKVITVFFSVMIGAFSLGQGAPNLESVAKARGAAYSIYNTIDMPRPIDSSSKEGHKPDSVRGDIEFKNIHFSYPSRKDVKILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDAGEVTLDGRDIRSLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDATDQDIDRAVREANAYEFISKLPDGLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSES--QGNFPN--ARAGRTTIVIAHRLSTIRTAD 503          

HSP 2 Score: 320.857 bits (821), Expect = 1.194e-96
Identity = 180/528 (34.09%), Postives = 290/528 (54.92%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVS-----PRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA +P   ++F  ++ +         R  TM FS       + F ++ +  FI+ F Q      +    + RLRS  F A+LRQ+I WFD      G L TKL  D   ++     ++      + +    +I+AF   W+L+ +    +P +     + +  ++ ++ K   A   +G I+ E + + +TVVA + ++   ++++ +L E     + ++   G+   +     + V + V+  G   I         V  +F  I+  ++++G +     +F  AK AA  +  ++++ P+I  + + G  P +F GDIEF  + F YPTRP + ILQ  N ++  G+T+A +G SG GKST I LL+RFY+P  G++   G D + ++L W RS +G+V QEP LF  +I ENIQ G    +   EE+ ++ +++N +EFI+   +++NT +GD+G   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SEK+VQK LD   A   RT +++AHRL TV+NAD
Sbjct:  607 GAVYPCVGILFAKIIGVFAETDPEVKRQKTMMFS-------LLFLLVGAVAFITYFFQGYMFGKSGEILTMRLRSQAFKAILRQDISWFDDHNNAVGVLTTKLATDASLVKGVTGSRIGLATSSVCALGISVIIAFCFSWQLTLLILACVPFLTGANFIQMRAMQGHASKDQSALETSGKISTETVENFKTVVALTKEDVFFRKFNDSLAEPYKSALCKAPIYGITFALAQAIPYLVNAAVFRFGAWLIAHCHTQYENVFLVFSVIIFAAMSIGQSSSFAPDFAKAKVAAGRILSLLEKTPEIDIYSEAGDKPMNFKGDIEFHELHFSYPTRPNIKILQGLNVSVRQGQTLALVGGSGCGKSTSIQLLERFYNPAGGQVFADGMDTRMLNLAWLRSQLGLVSQEPILFDCTITENIQYGDNSRVVSQEEVEEAAKNANIHEFILNLPERYNTRVGDKGTQLSGGQKQRIAIARALVRNPKVLLLDEATSALDTESEKIVQKALDD--ARQGRTCIVIAHRLTTVQNAD 1125          
BLAST of SMED30003432 vs. Ensembl Cavefish
Match: abcb5 (ATP-binding cassette, sub-family B (MDR/TAP), member 5 [Source:ZFIN;Acc:ZDB-GENE-030131-6414])

HSP 1 Score: 417.927 bits (1073), Expect = 3.325e-132
Identity = 218/543 (40.15%), Postives = 334/543 (61.51%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVN-LLVSPRDLT----------------MTF-SERMRPSVIK----FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGL-LFIYE-GSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDR-VPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GAA P+  ++F  + +  ++S + L                 MTF  E +  S+ K    F  + +   + G  Q++   +T+ RQ++R+R  YF+A+L Q + WFD     +L T+LTDD+ +I  G+ DK+  F+Q    F+   I+ F  GWKL+ +     P++A + +L    +   + +++ AY+ AG ++ EIL +IRTVVAF+ ++K +++Y  NL EA+  GV+++I      G+  F +F+  +L ++ G  L + E  +Y  G V+ +F +++IG+++LG   P LE+   A+ AAY ++  ID   P  S    G  P+S  GDIEFK + F YP+R  V ILQ  +  +P+GKT+A +G SG GKST I LLQRFYDP AGE++  G D++ +++ W R ++G+V QEP LF  +I ENI+ G+ DA +++I ++ +++NAYEFI K     NT +G+RGA  SGGQKQRIAIA ALV+NP++LLLDEATSALD  SE +VQ  LDK  A   RT +++AHRL T+R AD
Sbjct:   12 GAALPIMFIVFGSMTDSFILSGQQLNFTGNYTFNLTKRSSDCMTFPGEDIEGSMTKNAYYFVGIGAGVLLLGTFQVMLFVITSTRQTKRIREKYFHAVLHQPMAWFDTHPIRELNTRLTDDINTINDGLGDKICIFVQYFCRFLAGFIIGFIYGWKLTLVIMSVSPLLAGSAALCSKILATLTGRELSAYAKAGSVSAEILGAIRTVVAFNGRQKALEKYETNLLEAKNFGVRKAITTNAAMGITQFLLFSTFALAFWYGTKLSVDEPENYSIGKVLTVFFSVMIGALSLGQGAPNLESIAKARGAAYSIYSTIDMPRPIDSSSIKGYKPDSVSGDIEFKKIHFSYPSRKDVKILQGMSLKVPHGKTIALVGASGCGKSTTIQLLQRFYDPDAGEVTLDGRDIRCLNVRWLRENMGIVSQEPVLFGTTIAENIRYGREDATDQDIDRAVREANAYEFISKLPDGLNTMVGERGAQLSGGQKQRIAIARALVKNPKILLLDEATSALDTQSEAIVQAALDK--ARAGRTTIVIAHRLSTIRTAD 552          

HSP 2 Score: 326.25 bits (835), Expect = 3.202e-98
Identity = 186/531 (35.03%), Postives = 294/531 (55.37%), Query Frame = -1
Query:    1 CVGAA-FPLNVLIFRDVVNLLVS-----PRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDK--QQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1560
            CVG   +P   ++F  ++ +         R  +M FS       + F ++ +  F++ F Q   +  +    + RLRS  F A+LRQ+I WFD      G L TKL  D   ++     +L+       S    +I+A    W+L+ +    +P +     +    +K ++ K  +A   +G I+ E + + RTVVA + +E  ++++S +L E     + ++   G+   +   + + V +  +  G   I         +  +F  +V+ ++ +G       +F  AKAAA  +  ++++ P+I  +   GV P +F GDIEF +V F YPT+P V ILQ  N ++  G+T+A +G SG GKST I LL+RFY+P  G++   G D + ++L W RS +G+V QEP LF  +I ENIQ G    +   EE+V++ + +N +EFI+   +++NT +GD+G   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SEK+VQK LD+  A   RT +++AHRL TV+NAD
Sbjct:  689 CVGGGVYPCVGILFAKIIGVFAELDPEVKRQKSMMFS-------LLFFLVGTMAFLTYFFQGYMLGKSGESLTMRLRSQAFKAILRQDISWFDDYDNAVGVLTTKLATDASLVKGAAGSRLSLATSSFCSLGVSVIVALCFSWQLTLLILACVPFLTGAMFIQTRSMKGHASKDQRALETSGKISTETVENFRTVVALTKEEVFMQKFSDSLSEPYKSALWKAPLYGMSFALAQASSYFVRAAFFRFGAWLIAHCYAQYEDIYLVFSVLVLSTMNVGQTSSANLDFAKAKAAAGRIIALLEKKPEIDIYSDAGVKPTNFRGDIEFHDVHFSYPTQPDVRILQGLNVSVRQGQTLALVGGSGCGKSTSIQLLERFYNPAGGQVFADGMDTRGLNLAWLRSQLGLVSQEPILFDCTITENIQYGDNSRVVSQEEVVEAAKKANIHEFILNLPERYNTRVGDKGTQLSGGQKQRIAIARALVRNPKVLLLDEATSALDTESEKIVQKALDE--ARKGRTCIVIAHRLTTVQNAD 1210          
BLAST of SMED30003432 vs. Ensembl Cavefish
Match: abcb11a (ATP binding cassette subfamily B member 11 [Source:NCBI gene;Acc:103030940])

HSP 1 Score: 417.927 bits (1073), Expect = 8.401e-132
Identity = 239/586 (40.78%), Postives = 356/586 (60.75%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISG--------------------------------------FIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMG-LLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V ++QLFRFAT  D+++++V  + ++  GAA PL +L++  + N+ V+     +   E   P   K  I ++ T+ +G                                      + Q+      AARQ++R+R  YF  ++  EI WFD    G+L TK++DD+  I + I+D++  FI++IS+F+   ++ F  GWKL+ +     P++     LM   + + + ++++AY+ AG IA+E+LSSIRTV AF  ++KE +RY  NL+EA+T GVK+    G+  G +   IF   +L ++ G  L I      PGT+IQ+F  ++IG++ LG A P LE F + +AAA  +F  IDR+P+I    + G   E   GDIEF +++F YP+RP V IL N N  +  G+T AF+GPSGSGKST + L+QRFYDP  G ++  G D++ +++ W RS IG+V+QEP LF+ SI ENI+ G+     EEI+++++++NAY FIM   QKF+T +G+ G   SGGQKQRIAIA ALVRNP++LLLD ATSALDN SE +VQ+ LDK  A   RT + +AHRL T+RNAD
Sbjct:   31 VGFFQLFRFATCTDIVMMVVGGLCALVHGAASPLMLLVYGMMTNIFVA---YELEVQELADPD--KTCINNTVTWTNGSVVELEGNTVYCGVDIEAQMTMFAYYYIGIGLGVLILSYFQIALWVSAAARQTQRIRKTYFRKIMCMEIGWFDCTSVGELNTKISDDINKINNAIADQVTIFIERISTFIFGFMVGFVGGWKLTLVIIAVSPLIGLAAGLMAMAVARLTGRELQAYAKAGAIADEVLSSIRTVAAFGGEDKETERYDKNLEEAQTWGVKKGTIIGIFQGYLWCIIFLCYALAFWYGSKLVIDTKELTPGTLIQVFFGVLIGAMNLGQASPCLEAFASGRAAAKSIFDTIDRIPKIDCFSEEGHKLEKVKGDIEFHSIEFNYPSRPDVKILDNLNLEVKAGETTAFVGPSGSGKSTAVQLIQRFYDPSQGMVTLDGHDIRSLNIQWLRSLIGIVEQEPVLFATSISENIRYGRPGVTMEEIIQASKEANAYNFIMDLPQKFDTLVGEGGGQMSGGQKQRIAIARALVRNPKILLLDMATSALDNESEAVVQEALDK--ARVGRTTITIAHRLSTIRNAD 609          

HSP 2 Score: 296.975 bits (759), Expect = 1.722e-87
Identity = 183/527 (34.72%), Postives = 289/527 (54.84%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDK--QQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRS----ICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G+  P+  L+F  ++    + RDL     +++    I F I+   +F S F+Q    + +  R +RRLR   F A+L+QE+ WFD      G L T+L  D   ++     ++   +  +++     I+A+T  WKLS + +  +P++  +       +  ++ +  KA   AG ++ E +++IRTV   + +++ V  Y   L+       K++    IC      VI        +   Y G L  YEG +    V ++   IV    ALG A     ++  AK AA   F+++DRVP+I+ ++K G   + F G +EFKN  F YP+RP + +L+     +  G+T+AF+G SG GKST + LL+RFYDP  G++   G    D+++ + RS IG+V QEP LF  +I ENIQ G    +   +EIV +++ +  +EF+M    K+ T +G +G+  S GQKQRIAIA A++RNP++LLLDEATSALD  SEK VQ  LD+  A   RT +++AHRL T++ AD
Sbjct:  756 GSVNPIYALLFSQILGTF-ALRDLEEQ-RDQINGVCILFVIVGCMSFFSQFLQGYAFAKSGERLTRRLRKVGFQAMLQQEVGWFDDPTNSPGALTTRLATDASMVQGATGSQIGMIVNSLTNIGASFIIAYTFSWKLSLVVTGFLPLIGLSGVFQAKMLTGFANEDKKALEAAGQVSSEAMANIRTVAGLAKEKQFVDLYEQQLEAPYRAAKKKANIYAICFAFAQCVI---FMAYAASFRYGGYLVTYEGLHYV-VVFRVIAAIVTSGTALGKASSFTPDYAKAKIAAAQFFKLLDRVPRININQKDGKRWDHFKGRVEFKNCTFTYPSRPSIQVLRGLEVAVSPGQTLAFVGSSGCGKSTSVQLLERFYDPDQGQVLIDGHPSHDVNVPFLRSQIGIVSQEPVLFDCTIAENIQYGDNSRLISMDEIVDASKKAYLHEFVMSLPDKYETQVGAQGSQLSRGQKQRIAIARAIIRNPKILLLDEATSALDTESEKTVQAALDE--ARRGRTCIVIAHRLSTIQTAD 1274          
BLAST of SMED30003432 vs. Ensembl Cavefish
Match: abcb11b (bile salt export pump-like [Source:NCBI gene;Acc:103028308])

HSP 1 Score: 412.92 bits (1060), Expect = 1.015e-129
Identity = 227/584 (38.87%), Postives = 352/584 (60.27%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIF---------------------RDVVNLLVSPRDLT---------------MTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEG-SYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISH-KKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            + ++QLFRFAT  ++ +++   + ++  G+A PL +L+F                     +  +N  +  R+L+               +     M      +  + S  FI G++Q+     +AARQ + +R  YF  ++R EI WFD    G+L T+++DD+  I   I+D+++ FIQ+ ++FV   ++ F  GWKL+ +     P++    +LM  F+ K + ++++AY+ AG +A+E+LSSIRTV AF  ++KEV+RY HNL  A+  G+++ +  G  +G + F IF   +L ++ G   + +   Y PGT++Q+F  ++I ++ LG A P LE F   + AA  +F+ IDR P+I    + G   +   GD+EF NV F YP+RP V IL   N  +  G+T AF+GPSG+GKST I LLQRFYDP  G ++  G D++ +++ W RS +G+V+QEP LF+ +I ENI+ G+    +E+I+K+ +++NAY FIM   QKF+T +G+ G   SGGQKQRIAIA ALVRNP++LLLD ATSALDN SE +VQ+ LDK      RT + +AHRL T++NAD
Sbjct:   56 IGFFQLFRFATCREVCMMVCGSVCAVLHGSAQPLMLLVFGLLTDTFIEYDIELNELTDPEKRCINNTIQWRNLSAEENLELNMTRTCGLLDIEYEMTNFAYYYVGIGSGVFILGYLQISLWVTSAARQIQIIRRLYFGKVMRMEIGWFDCNSVGELNTRMSDDINKINDAIADQVSIFIQRFTTFVCGFLMGFVRGWKLTLVIISVSPLIGVGAALMALFVAKLTGRELQAYAKAGAVADEVLSSIRTVAAFGGEKKEVERYDHNLISAQRWGIRKGLIMGFFTGYLWFIIFLCYALAFWYGSSLVVDTLEYSPGTLLQVFFGVLIAALNLGQASPCLEAFAAGRGAATTIFETIDREPEIDCLSEAGYKLDRVKGDLEFHNVTFHYPSRPEVKILDQLNLQVKTGETTAFVGPSGAGKSTAIQLLQRFYDPKEGMVTLDGHDIRGLNIQWLRSLMGIVEQEPVLFATTIAENIRYGRPGVSHEDIIKAAKEANAYNFIMDLPQKFDTLVGEGGGQMSGGQKQRIAIARALVRNPRILLLDMATSALDNESEAIVQEALDKV--RLGRTTISIAHRLSTIKNAD 637          

HSP 2 Score: 269.626 bits (688), Expect = 9.673e-78
Identity = 164/485 (33.81%), Postives = 265/485 (54.64%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F ++   +F +  +Q  + S +    +RRLR   F A+L QE+ WFD  +   G L T+L  D   ++     ++   +  +++    II++F   WKLS +    +P +A +       +  ++ +  +A   AG I+ E L++IRT+     + + V+ Y   L+      +K++   G   G     +F   S  Y  G   +         V ++   IV    ALG A     ++  AK +A   FQ++DR P+IS +   G     F GDIEF +  F YP+RP + +L     ++  G+T+AF+G SG GKST + LL+RFYDP  G++   G +   I++ + RS IG+V QEP LF  SI ENI+ G    +   ++++ +++ +  ++F+M   +K+ T +G +G+  S GQKQRIAIA A++R+P++LLLDEATSALD  SEK VQ+ LDK  A   RT +++AHRL T++N+D
Sbjct:  829 FVLVGVISFFTQMLQGYSFSKSGELLTRRLRRLGFQAMLGQEVGWFDDHRNSPGALTTRLATDASQVQGATGSQIGMIVNSLTNIGVAIIISFYFSWKLSLVIMCFLPFLALSGGFQAKMLTGFAKQDKQAMEAAGQISGEALNNIRTIAGLGKERRFVEMYEAQLEAPYRAALKKANVYGACYGFAQCVVFMANSASYRFGGYLVRHEGLHFSLVFRVISAIVTSGTALGRASSYTPDYAKAKISAARFFQLLDRTPKISVYSDKGDKWNHFKGDIEFIDCKFTYPSRPDIQVLNGLTVSVKPGQTLAFVGSSGCGKSTSVQLLERFYDPDFGKVLIDGRESSRINVQFLRSKIGIVSQEPILFDCSILENIRYGDNKRVLSMDDVISASKKAQLHDFVMSLPEKYETNVGSQGSQLSRGQKQRIAIARAIIRDPKILLLDEATSALDTESEKTVQEALDK--AREGRTCIVIAHRLSTIQNSD 1311          
BLAST of SMED30003432 vs. Ensembl Sea Lamprey
Match: abcb11b (ATP-binding cassette, sub-family B (MDR/TAP), member 11b [Source:ZFIN;Acc:ZDB-GENE-050517-14])

HSP 1 Score: 395.586 bits (1015), Expect = 4.181e-124
Identity = 228/579 (39.38%), Postives = 346/579 (59.76%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLV----------SPRDLTMTFS-----------------ERMRPSVIKFAI----LSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGL-LFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V   QLFRFA G D+++++   + +I  GAA P  +L+F  + +  +           P  + +  S                   +   +  FA     +     + G++Q+    + AARQ  R+R  +F+ ++R EI WFD    G+L T+++DD+  I   I+DK++ FIQ+ ++FV   ++ F  GWKL+ +     P++    +LM  F+ + + +++KAY+ AG +AEE+LSSIRTV AF  ++KE  RY  NL  A+  G+++ +  G   G + F IF   +L ++ G  L + E  Y PGT++Q+F  +++G+I LG A P LE F + + AA  +F +IDR P I      G I +   GDIEF NV F YPTR  +  L +FN  +  G+T AF+GPSG+GKST + L+QRFYDP  G+++  G D++ ++L W RS IGVV+QEP LF+ +I ENI+ G+ +    E+ ++ + +NAY FIM+   KF+T +G+ G   SGGQKQRIAIA ALVRNP++LLLD+ATSALDN SE +VQ  LDK + +G  T + +AHRL T+ +AD
Sbjct:   51 VGLCQLFRFAKGGDIVLMVTGALGAIVHGAALPFMLLVFGRMADAFIEYDLETQELQDPNKMCLNNSILWVNGTTIPNATACGHTDIEVELTTFAFYYLGIGCVVLLVGYLQICFWVMAAARQVLRIRQAFFHNIMRMEIGWFDMNSVGELNTRISDDINKINDAIADKMSIFIQRFTTFVCGFLIGFLNGWKLTLVILAVSPLLGLGGALMGSFVARMTGRELKAYAKAGAVAEEVLSSIRTVAAFGGEKKEENRYDKNLVMAQKWGIRKGMILGFFQGYMWFIIFLCYALAFWYGSNLVLEEEEYSPGTLLQVFFGVLVGAINLGQASPCLEAFASGRGAATHVFNIIDREPIIDCMSDDGNILQKVKGDIEFHNVKFSYPTRLDLTALHSFNLKVNAGETTAFVGPSGAGKSTAMQLIQRFYDPQEGKVTLDGHDIRSLNLRWMRSLIGVVEQEPILFATTIAENIRYGRDNVTVSEVEQAARRANAYNFIMELPHKFDTMVGEGGGQMSGGQKQRIAIARALVRNPRILLLDQATSALDNESEAVVQSALDK-VRKGC-TTISIAHRLSTIHSAD 627          

HSP 2 Score: 265.388 bits (677), Expect = 4.510e-77
Identity = 171/537 (31.84%), Postives = 275/537 (51.21%), Query Frame = -1
Query:    1 VGAAF-----PLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNL--------KEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGK--MDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1557
            VGAA      P+  ++F +++     P +        +  + + F ++   +F++ F+Q    S +    +RRLR   F A+LRQ++ WFD  K   G L T+L  D   ++      +  F  ++           +  W+              TF + I+    Y        + A  + E I++ IRTV     +   VK+Y+ +L        ++A   G   S  Q +         FT  +   Y G L  ++G   P  V ++F  +V    ALG A     ++  AK +A  LF++ DR P I S    GV  + F GD+ F N  F YP+RP   +L   + ++  G+T+A +G SG GKST + LL+RFYDP  G++   G D + +++ + RS IG+V QEP LF  SI +NI+ G    +   +E++ +++ +  ++F+M   +K+ T +GD+GA  S G+KQR+AIA A++R+P++LLLDEATSALD  SE+ VQ  LD+  A   RT +++AHRL T++NAD
Sbjct:  759 VGAAINGGIQPVFAILFSEILGTFTLPTEEEQ--RNMINLTCLLFVLIGIVSFVTYFLQGYMFSKSGELLTRRLRRCGFTAMLRQDVGWFDDLKNSPGALTTRLATDASQVQGVKICYVFEFHSRMFFSFSSSCCCCSHRWERQ------------TFIMNIYIRTPYGEDNRTRNARAHFVNESIVN-IRTVAGLGKERTFVKQYTDSLTVPHRAAIRKANVFGATFSFSQSV-------IFFTYAASFRYGGFLVAHDGLKFP-LVFRVFSALVFSGTALGRASSYAPDYAKAKISAARLFKLFDRTPAIDSSPDKGVSWDGFRGDVTFTNCTFTYPSRPDTQVLNGLSVSVQPGQTLALVGTSGCGKSTSVQLLERFYDPDGGQVLIDGRDSRTVNVGFLRSKIGIVSQEPVLFDCSIADNIRYGANFRELTWDEVINASKAAQLHDFVMGLPEKYETNVGDKGAQLSRGEKQRVAIARAIIRDPRILLLDEATSALDTESEQTVQSALDQ--ARQGRTCIVIAHRLSTIQNAD 1270          
BLAST of SMED30003432 vs. Ensembl Sea Lamprey
Match: ABCB1 (ATP binding cassette subfamily B member 1 [Source:HGNC Symbol;Acc:HGNC:40])

HSP 1 Score: 313.153 bits (801), Expect = 4.306e-95
Identity = 168/383 (43.86%), Postives = 237/383 (61.88%), Query Frame = -1
Query:    1 PIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEG-SYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRV----PQISHK---KHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1125
            P++A +  L    +  ++ +++ AYS AG +AEE+LS+IRTV+AF  QEKE+KRY  NL EA  +G+K++I   L  G   F ++   SL ++ G   + E  SY  G V+ +F  ++IG+  +G A P LE    A+ AAY +F++IDR     P  +H    ++      F       ++ F         +L+  N  +  G+TVA +G SG GKST I LLQRFYDP  G I   G D++ ++L   R  IGVV QEP LF+ +I ENI+ G      E+I ++ +++NAY+FI +  ++FNT +GDRGA  SGGQKQRIAIA AL+RNP+LLLLDEATSALD  SE +VQ+ LDK  A   RT +++AHRL T+RNAD
Sbjct:    9 PVLAISAGLWSKILTSFTNRELLAYSKAGAVAEEVLSAIRTVMAFGGQEKELKRYDKNLVEARNIGIKKAITTNLSIGFTMFIVYASYSLAFWYGSRLVLEDTSYTIGNVMIVFFAVIIGAFGIGQAAPFLEAISVARGAAYTIFEIIDRTIHMYPFNTHNSSLRNRKRCTWFREHTLVNSIAF-------CQVLKGVNLQVKSGQTVALVGSSGCGKSTTIQLLQRFYDPQQGTIMVDGRDVRTLNLRSLRQLIGVVSQEPVLFATTIAENIRFGHEKVTMEDIERAARNANAYDFISQMPERFNTLVGDRGAQLSGGQKQRIAIARALIRNPKLLLLDEATSALDGESEAIVQEALDK--AREGRTTIVIAHRLSTIRNAD 382          

HSP 2 Score: 310.457 bits (794), Expect = 3.913e-94
Identity = 181/523 (34.61%), Postives = 283/523 (54.11%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GA  P   +IF  ++ +  +  DL     +    S++ F +L   +F++ F+Q      +    + +LRS    A+LRQ++ WFD  K  TG L T+L  D   ++     +LA   Q I++    I+++F  GW+L+ +    +P +     L +  +   + +  K    AG IA E + +IRTV + + +    K Y  +L+       K+S   GL  G     ++   +  +  G   +  G  +   V  +F  IV G++A+G       ++  AK +A  LF +   +P I S+   G   E   G++EF++V F YP RP V +L+  +  +  G+T+A +G SG GKST + LL+RFYD L+G +   G D++ + + W R+ +G+V QEP LF  S+ ENI  G    I   +EIV + + +N + FI    +K+NT +GD+G   SGGQKQRIAIA ALVR P++LLLDEATSALD  SEK+VQ+ LD+  A   RT +++AHRL TV+ AD
Sbjct:  506 GALQPAFAVIFSRIIGVF-AETDLVKREEQSNLYSLL-FLVLGIVSFVTFFLQGFLFGRSGELLTMKLRSMGLRAMLRQDMMWFDDPKNSTGALTTRLATDASQVQGATGARLATIAQNIANMGTAIVISFVYGWELTLLILAIVPFVGAAGFLEMQALTGQANRDKKLLEEAGKIATEAVENIRTVASLTRERTLEKIYQDSLEGPAKTAKKKSQFYGLTYGFSQGVVYLAQAAAFRFGAFLVTAGRMEFENVFLVFSAIVFGAMAVGQTSSFAPDYAKAKTSANHLFYLFQLIPAIDSYSDEGEKLEDCEGNVEFQSVGFNYPMRPNVEVLKGLSLKVENGQTLALVGSSGCGKSTSVQLLERFYDTLSGNVLIDGKDVRSLHIQWLRAQMGIVSQEPVLFDCSMAENIAYGDNSRIVPQDEIVAAARAANIHTFIDSLPEKYNTKVGDKGTQLSGGQKQRIAIARALVRQPKILLLDEATSALDTESEKIVQEALDR--AREGRTCIVIAHRLSTVQTAD 1024          
BLAST of SMED30003432 vs. Ensembl Sea Lamprey
Match: abcb8 (ATP-binding cassette, sub-family B (MDR/TAP), member 8 [Source:NCBI gene;Acc:548340])

HSP 1 Score: 298.13 bits (762), Expect = 4.395e-93
Identity = 176/493 (35.70%), Postives = 281/493 (57.00%), Query Frame = -1
Query:    1 VIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYS----HNLKEAETLGVKRSICQGLGSGVIGF----TIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPE-SFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSH-IGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1449
            ++ +    + TF  G+I +  +S    R +  +R + F AL+RQ++ +FD  +TG LV +LT DV+  +S     ++  ++ ++  VGC+   +    KL+ +  + +  M    +L+  F++K S +  +  S A G+A+E L ++RTV AF+ ++ + ++Y+     + K  E LG+  +I QGL + V+      TIF  GSL+    L         PG ++   +       ++ +   +    +   +A   LF+ ++  P I  +  G+IP  S  G + F NV F YPTRPG  +L NFN  LP  +T+A +G SG GKST+  LL+RFYDP +G I+  G DL+ +D +W R   IG + QEP LF  +I ENI+ GK  A ++E+  + + +NA +FI      +NT +G+RGA  SGGQKQR+AIA AL++ P +L+LDEATSALD  +E  VQ+ L++  A   RTV+++AHRL TV  A+
Sbjct:  109 LVMYGFQGALTF--GYIAL--LSQVGERIAASMRKSLFEALIRQDVAFFDANRTGHLVNRLTSDVQEFKSSFKLVISQGLRSVTQTVGCVASLYLISPKLTGLMVVVMTAMVGGGALIGSFLRKLSRRAQEQISKATGVADETLGNVRTVRAFAMEDSKSEQYAVEVDKSCKMNEDLGIGIAIFQGLSNIVLNCIVLGTIFVGGSLMAGEEL--------SPGELMSFLVASQTVQRSMASVSILFGQMVRGLSAGARLFEYMELEPSIPVRGGGIIPHHSCLGHVRFNNVTFSYPTRPGQVVLHNFNLNLPPSQTIAIVGLSGGGKSTVAALLERFYDPASGSITLDGKDLRTLDPSWLRGRVIGFINQEPVLFGTTIIENIRFGKPGATDDEVYAAARLANADDFIRSFPDGYNTVVGERGATLSGGQKQRVAIARALIKQPAVLVLDEATSALDAEAEGAVQEALER--AAHGRTVLVIAHRLSTVAGAN 587          
BLAST of SMED30003432 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001786.1 (pep scaffold:Pmarinus_7.0:GL477870:201625:214977:-1 gene:ENSPMAG00000001622.1 transcript:ENSPMAT00000001786.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 291.197 bits (744), Expect = 3.872e-92
Identity = 164/451 (36.36%), Postives = 259/451 (57.43%), Query Frame = -1
Query:    1 QEICWF--DKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIM--ATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKR----SICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI--NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1320
            QEI WF   K  TG L  +L+ D   ++     +    +Q +S     +++A   GWKL+ +    +P M  A     ++   +  +IK  +AY  A  I+ E + +IRTV +   +EK    +   L E   +  +R    S+  G   G++ F      + V+ +G   + +G      V ++F  I+ G++A+G A+ M  N+  AK +A  +  ++ R   I S+ + G+ P++F G+++   V F YPTRP   +L+  +  +  G+T+A +GPSGSGKSTI+ LL RFYD  AG++  +G +++ ++L W R  +GVV QEP LF  SI ENI  G +      +E+V + + +N ++F+    Q ++T +G+RGA  SGGQKQR+AIA AL+R+P++LLLDEATSALD  SEK+VQ  L+K  A+  RT +++AHRL TVR AD
Sbjct:    7 QEIRWFYNHKNNTGALTAQLSTDAAQVQGATGRRAGMLVQNVSGLATGLLIALLVGWKLALLMLAFMPFMVGAGLVQSVLQGSEATTIK--RAYEQASKISTEAVQNIRTVASLCLEEKFSGMFEQCLLEPYRVIKRRGYLFSVTFGFTQGILCFAY----AAVFRLGGFLVAQGELTFQDVYKVFGAIIFGALAVGQAMAMAPNYGRAKVSAARILALLRREMAIDSYSEGGIQPDAFDGELQLSGVHFRYPTRPHAEVLRGVSLRVAPGQTLALVGPSGSGKSTIMQLLLRFYDSSAGQLLLNGVEVRQLNLGWLRRQLGVVSQEPVLFGCSIAENIAYGDVSRAVSRDEVVAAARAANLHDFVESLPQAYDTVVGERGAQLSGGQKQRVAIARALLRDPRVLLLDEATSALDAESEKVVQDALEK--AQQGRTCIVIAHRLSTVRAAD 449          
BLAST of SMED30003432 vs. Ensembl Sea Lamprey
Match: abcb9 (ATP-binding cassette, sub-family B (MDR/TAP), member 9 [Source:ZFIN;Acc:ZDB-GENE-050517-12])

HSP 1 Score: 270.781 bits (691), Expect = 3.135e-81
Identity = 157/486 (32.30%), Postives = 265/486 (54.53%), Query Frame = -1
Query:    1 SVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVG-SLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVI-PESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1452
            +++  ++++  + I+  ++    +LT +R + R+R+  F +L+ QE+ +FD  +TG + ++L  D   +   +S     F++ +    G  I  F   W+LS +  + +PI+         F +K S     + ++A G AEE L++IRTV + + +E E +RY   L+       ++++     S     T   +   ++YY G L + +       +  I   + +G+  L N   +    + A  AA  +F+ ++R P++  +  G + P    G + FK+V F YP+RP  P+L+  +F L  G   A +GPSGSGKS+ +HLLQR Y+PL G +   G  +      +  + + +V QEP LF+ S+++NI  G  D     ++ ++Q +NA+EFI      + T  G++GA  SGGQKQR+AIA ALVR+P++L+LDEATSALD  SE +VQ+ L      G RTV+++AHRL +V  AD
Sbjct:  252 AILYMSLIAIASSIAAGLRGGLFTLTMSRLNIRIRNLLFRSLIHQEVAFFDTTRTGDITSRLNSDTTVVSDVVSQNANVFLRSLVKAGGVCIFMFGLSWRLSLLTLLGLPIVTLVSKFYGDFYEKLSKAVQDSLAVASGAAEETLAAIRTVRSLAGEEAECRRYRDALQHTYRHNRRQALAYAGFSCSNSLTQLALQVGILYYGGHLVVTDRISGAHLISFILYQMELGA-CLENMGSVYTGLMQAVGAAGKVFEYMEREPRM--RTDGTLAPVQLEGQVSFKDVTFAYPSRPDAPVLKGVSFELQPGLVTALVGPSGSGKSSCVHLLQRLYEPLGGRVLLDGQSVHKYQHHYLHTKVALVGQEPVLFARSLRDNIAYGMEDCPENAVISASQQANAHEFISDLPLAYQTEAGEKGAQLSGGQKQRVAIARALVRDPRVLILDEATSALDAESEHMVQQALYNGELRG-RTVLVIAHRLSSVERAD 733          
BLAST of SMED30003432 vs. Ensembl Yeast
Match: MDL1 (Mitochondrial inner membrane half-type ABC transporter; mediates export of peptides generated upon proteolysis of mitochondrial proteins; plays a role in the regulation of cellular resistance to oxidative stress [Source:SGD;Acc:S000004178])

HSP 1 Score: 242.662 bits (618), Expect = 7.190e-72
Identity = 161/478 (33.68%), Postives = 255/478 (53.35%), Query Frame = -1
Query:    4 QMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDV----ESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAG--GIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGD-IEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENI------QLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNA 1398
            +++ + +T  R   RLR+    A L Q+  + D  + G L+++L+ D     +S+   +SD   A IQ    FVG  +++F + WKL+ +  +  P +     +    I+  S +Q++  S+ G   +AEE L++ RT+ A+  ++ EV+RY+  ++    +G+K ++  GL  G  G    T    +  +G   I  GS   G +    +  V    +L          +    AA  +F++ DR P I     G  P S     I FKNV F YPTRP   I ++ N T+  G+ V  +GPSGSGKSTI  LL R+YD  +G I F   D+++ +L  YR  IG VQQEP LF+G+I +NI      ++ + D   + I ++   +N  +F+        T +G RGA  SGGQKQRIA+A A + +P +L+LDEATSALD+ SE++V K L + +  G  T + +AHRL T++++
Sbjct:  173 RIIILKVTGERLVARLRTRTMKAALDQDATFLDTNRVGDLISRLSSDASIVAKSVTQNVSDGTRAIIQ---GFVGFGMMSFLS-WKLTCVMMILAPPLGAMALIYGRKIRNLS-RQLQT-SVGGLTKVAEEQLNATRTIQAYGGEKNEVRRYAKEVRNVFHIGLKEAVTSGLFFGSTGLVGNTAMLSLLLVGTSMIQSGSMTVGELSSFMMYAVYTGSSLFGLSSFYSELMKGAGAAARVFELNDRKPLI-RPTIGKDPVSLAQKPIVFKNVSFTYPTRPKHQIFKDLNITIKPGEHVCAVGPSGSGKSTIASLLLRYYDVNSGSIEFGDEDIRNFNLRKYRRLIGYVQQEPLLFNGTILDNILYCIPPEIAEQD---DRIRRAIGKANCTKFLANFPDGLQTMVGARGAQLSGGQKQRIALARAFLLDPAVLILDEATSALDSQSEEIVAKNLQRRVERG-FTTISIAHRLSTIKHS 639          
BLAST of SMED30003432 vs. Ensembl Yeast
Match: MDL2 (Mitochondrial inner membrane half-type ABC transporter; required for respiratory growth at high temperature; localizes to vacuole membrane in response to H2O2; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis [Source:SGD;Acc:S000006191])

HSP 1 Score: 236.884 bits (603), Expect = 4.228e-69
Identity = 165/492 (33.54%), Postives = 252/492 (51.22%), Query Frame = -1
Query:    1 AILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYE-GSYDPGTVIQIFLTI------VIGSIALGNAVPMLENF----ITAKAAAYPLFQVIDRVPQISHK-KHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG-KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1437
            A+L  C   + F + + + + + R   RLR+N     L Q+  +FD  + G L+++L  D   +   ++ K++  ++ +   V  + +  +   +LS +     P +  + S+    I+  S    +A      +AEE LS I+TV +F A+  E+ RY+  +++   +G   +              FT  SL+  +  L +   GSY    V+Q  L+I      ++ +   GNAV  L  F    +    AA  LF++ DR P IS    H   P+   G IEFK+V F YPTRP V I +N NF +  G +V  +GPSG GKSTI  LL R+Y+P  G I+    D+  ++    R HIG+VQQEP L SG+I++NI  G       EEI    +    + FI K    ++T +G  G   SGGQKQRIAIA AL++ P +L+LDEATSALD  SE  +  T  + +     T+V +AHRL T+R ++
Sbjct:  177 ALLIGCA--ANFGRFILLRILSERVVARLRANVIKKTLHQDAEFFDNHKVGDLISRLGSDAYVVSRSMTQKVSDGVKALICGVVGVGMMCSLSPQLSILLLFFTPPVLFSASVFGKQIRNTSKDLQEATGQLTRVAEEQLSGIKTVQSFVAEGNELSRYNVAIRDIFQVGKTAAFTNA--------KFFTTTSLLGDLSFLTVLAYGSY---LVLQSQLSIGDLTAFMLYTEYTGNAVFGLSTFYSEIMQGAGAASRLFELTDRKPSISPTVGHKYKPDR--GVIEFKDVSFSYPTRPSVQIFKNLNFKIAPGSSVCIVGPSGRGKSTIALLLLRYYNPTTGTITIDNQDISKLNCKSLRRHIGIVQQEPVLMSGTIRDNITYGLTYTPTKEEIRSVAKQCFCHNFITKFPNTYDTVIGPHGTLLSGGQKQRIAIARALIKKPTILILDEATSALDVESEGAINYTFGQLMKSKSMTIVSIAHRLSTIRRSE 653          
BLAST of SMED30003432 vs. Ensembl Yeast
Match: ATM1 (Mitochondrial inner membrane ATP-binding cassette (ABC) transporter; exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol; human homolog ABCB7 can complement yeast null mutant [Source:SGD;Acc:S000004916])

HSP 1 Score: 204.142 bits (518), Expect = 8.555e-58
Identity = 145/457 (31.73%), Postives = 237/457 (51.86%), Query Frame = -1
Query:    1 FNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQIS--SF---VGCIILAFTTGWKLSFIGSMSIPIMATTFSL-----MIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGV-IPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1338
            F  L++ ++ W   +QTG     LT  ++    GIS  L A +  I   SF   V C IL +  G   + I + S  ++ + F++       HF +  +    KA + A  +A + L +   V  F+ ++    +Y+ +L       +K S      +        T  + + YMG   +  G+   G ++ I   +   S+ L     +  +   +      LF++     +I + +  + +PE+   DI F+NV F Y   P   IL+N +FT+P G   A +G SGSGKSTI+ L+ RFYDP +G I  +G D+K+ D+   R  IGVV Q+ PLF+ +I EN++ G++DA +EE++   + +     I K  Q F+T +G+RG   SGG+KQR+AIA  L++N +++  DEATSALD  +E+ + +T+      G RT V +AHRL+T+ +AD
Sbjct:  195 FQHLMKLDLGWHLSRQTG----GLTRAMDRGTKGISQVLTAMVFHIIPISFEISVVCGILTYQFGASFAAI-TFSTMLLYSIFTIKTTAWRTHFRRDAN----KADNKAASVALDSLINFEAVKYFNNEKYLADKYNGSLMNYRDSQIKVSQSLAFLNSGQNLIFTTALTAMMYMGCTGVIGGNLTVGDLVLINQLVFQLSVPLNFLGSVYRDLKQSLIDMETLFKLRKNEVKIKNAERPLMLPENVPYDITFENVTFGY--HPDRKILKNASFTIPAGWKTAIVGSSGSGKSTILKLVFRFYDPESGRILINGRDIKEYDIDALRKVIGVVPQDTPLFNDTIWENVKFGRIDATDEEVITVVEKAQLAPLIKKLPQGFDTIVGERGLMISGGEKQRLAIARVLLKNARIMFFDEATSALDTHTEQALLRTIRDNFTSGSRTSVYIAHRLRTIADAD 640          
BLAST of SMED30003432 vs. Ensembl Yeast
Match: STE6 (Plasma membrane ATP-binding cassette (ABC) transporter; required for the export of a-factor, catalyzes ATP hydrolysis coupled to a-factor transport; contains 12 transmembrane domains and two ATP binding domains; expressed only in MATa cells; human homolog ABCB1 mediates multidrug resistance in many chemotherapy-resistant tumors by effluxing toxic compounds from the cell [Source:SGD;Acc:S000001692])

HSP 1 Score: 181.8 bits (460), Expect = 7.387e-49
Identity = 119/466 (25.54%), Postives = 236/466 (50.64%), Query Frame = -1
Query:    1 LRSNYFNALLRQEICWF--DKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLK-EAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDR------VPQISHKKHGVIPESFYGD-----IEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINE-EIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1353
            LR+     L R+ + WF  +  +  ++   + +D+  + S +S+ L+A    ++     +I A  +GWKLS +     P++    ++    ++K       + +       +I+++I+T+    A E   +   H+LK + + +  KR+I  G G  +    +  + +++YY GL  +    Y    +   F  ++   ++  + V  + +    + AA  +++++D       V   + +  G+   +++G      +  +N+ F YP+ P   + +N NF +  G+T+  IG SG+GKST++ LL + Y+   G+I   GTD+ D +LT  R  I VV+Q+P LF+G+I++N+  G  D I E E+  + +    ++F++ + Q  +T +       SGGQ QR+ IA AL+R  ++L+LDE TSALD++S  ++ + + K         +++ H  Q +R+ +
Sbjct:  795 LRNEVMEKLTRKNMDWFSGENNKASEISALVLNDLRDLRSLVSEFLSAMTSFVTVSTIGLIWALVSGWKLSLVCISMFPLIIIFSAIYGGILQKCETDYKTSVAQLENCLYQIVTNIKTIKCLQA-EFHFQLTYHDLKIKMQQIASKRAIATGFGISMTNMIVMCIQAIIYYYGLKLVMIHEYTSKEMFTTFTLLLFTIMSCTSLVSQIPDISRGQRAASWIYRILDEKHNTLEVENNNARTVGIAGHTYHGKEKKPIVSIQNLTFAYPSAPTAFVYKNMNFDMFCGQTLGIIGESGTGKSTLVLLLTKLYNCEVGKIKIDGTDVNDWNLTSLRKEISVVEQKPLLFNGTIRDNLTYGLQDEILEIEMYDALKYVGIHDFVISSPQGLDTRIDT--TLLSGGQAQRLCIARALLRKSKILILDECTSALDSVSSSIINEIVKK--GPPALLTMVITHSEQMMRSCN 1255          

HSP 2 Score: 177.563 bits (449), Expect = 1.788e-47
Identity = 134/476 (28.15%), Postives = 222/476 (46.64%), Query Frame = -1
Query:    1 RQSRRLRSNYFNALLRQE-ICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIM---ATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVID----RVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI---------NEEIVK-STQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1368
            RQ  R+RS    A L ++ + W+D  +   G   T++   VE + S  ++  A   Q + +    +  +F   W L+ I   S PI+   A  FS MIH    YS K+    S A  +    +++ + V  +  Q  E K++   +    T  +K        +G++ F   T+    ++ G   I +G  +   VI  F + ++    L N +  +        A   +  ++     R P      H    +    D+ F NV F YP+RP   +L+N +     G+    +G SGSGKST+ +LL RFYD   G IS +G +++ ID      +I VV+Q   LF+ ++++NI LG  D++         N  ++K + Q +    FI+       T +G  G   SGGQ+QR+AIA A +R+  +L LDEA SALD +   L+ K + +   +GK T+++  H L  + + D
Sbjct:  102 RQGFRIRSQILEAYLEEKPMEWYDNNEKLLGDF-TQINRCVEELRSSSAEASAITFQNLVAICALLGTSFYYSWSLTLIILCSSPIITFFAVVFSRMIHV---YSEKENSETSKAAQLLTWSMNAAQLVRLYCTQRLERKKFKEIILNCNTFFIKSCFFVAANAGILRFLTLTMFVQGFWFGSAMIKKGKLNINDVITCFHSCIMLGSTLNNTLHQIVVLQKGGVAMEKIMTLLKDGSKRNPLNKTVAHQFPLDYATSDLTFANVSFSYPSRPSEAVLKNVSLNFSAGQFTFIVGKSGSGKSTLSNLLLRFYDGYNGSISINGHNIQTIDQKLLIENITVVEQRCTLFNDTLRKNILLGSTDSVRNADCSTNENRHLIKDACQMALLDRFILDLPDGLETLIGTGGVTLSGGQQQRVAIARAFIRDTPILFLDEAVSALDIVHRNLLMKAI-RHWRKGKTTIIL-THELSQIESDD 571          
BLAST of SMED30003432 vs. Ensembl Yeast
Match: YOR1 (Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter mediates export of many different organic anions including oligomycin; homolog of human cystic fibrosis transmembrane receptor (CFTR) [Source:SGD;Acc:S000003513])

HSP 1 Score: 135.961 bits (341), Expect = 1.051e-33
Identity = 131/521 (25.14%), Postives = 238/521 (45.68%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVG-CIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKK--YSIKQIKAY--SIAGGIAEEILSSIRTVVAFSAQEKEVKRYS---HNLKEAETLGV--KRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGN----AVPMLENFITAKAAAYPLFQVIDRVP-QISHKKHGVIPESFY---GDIEFKNVDFEYPTRPGVPI-LQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINE----------------------EIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            F + ++  F++G  Q   +       S+ L       +L   + + D    G+++ + T D +S+++ +++ L     Q ++ VG C++      W      +++IP +   F L+    +     IK+++A   S       E+L  + T+ A+ +QE+ + +     + + EA  L V  +R +   L    I F +         + LL +          + + LT V+    L N    A+   EN + +   A  L      +P + S++K  + P   +   G+I F+NVDF Y  RPG+PI L+N N  +  G+ +   G +G+GKSTI+  L R  +  AG+I     D+  + L   R  + ++ Q+P LF G+I++N     +D  NE                      E+     D N     M  K   +  + + G+ FS G++Q +A+  ALVR  ++L+LDEATS++D  ++  +Q  + +    G  T++ +AHRL+T+ N D
Sbjct:  940 FFVFAAFIFMNG--QFTILCAMGIMASKWLNLRAVKRILHTPMSYIDTTPLGRILNRFTKDTDSLDNELTESLRLMTSQFANIVGVCVMCIVYLPW-----FAIAIPFLLVIFVLIADHYQSSGREIKRLEAVQRSFVYNNLNEVLGGMDTIKAYRSQERFLAKSDFLINKMNEAGYLVVVLQRWVGIFLDMVAIAFALI--------ITLLCVTRAFPISAASVGVLLTYVLQLPGLLNTILRAMTQTENDMNS---AERLVTYATELPLEASYRKPEMTPPESWPSMGEIIFENVDFAY--RPGLPIVLKNLNLNIKSGEKIGICGRTGAGKSTIMSALYRLNELTAGKILIDNVDISQLGLFDLRRKLAIIPQDPVLFRGTIRKN-----LDPFNERTDDELWDALVRGGAIAKDDLPEVKLQKPDENGTHGKMH-KFHLDQAVEEEGSNFSLGERQLLALTRALVRQSKILILDEATSSVDYETDGKIQTRIVEEF--GDCTILCIAHRLKTIVNYD 1432          

HSP 2 Score: 54.6842 bits (130), Expect = 4.029e-8
Identity = 43/190 (22.63%), Postives = 90/190 (47.37%), Query Frame = -1
Query:    4 QNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGK--MDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDN-ISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNA 564
            ++ NF +  G+ +   GP G+GKS++++ +        G++  +G    D+ +  Y          P + + S+++NI  G        +E+V+        + +        T +G+RG   SGGQK RI +A ++ +   + L D+  SA+D+ + + ++ + L   +A   +T ++  H+L  +  A
Sbjct:  605 KDLNFDIKKGEFIMITGPIGTGKSSLLNAMAGSMRKTDGKVEVNG----DLLMCGY----------PWIQNASVRDNIIFGSPFNKEKYDEVVRVCSLKADLDILPAGDM---TEIGERGITLSGGQKARINLARSVYKKKDIYLFDDVLSAVDSRVGKHIMDECLTGMLA--NKTRILATHQLSLIERA 775          
BLAST of SMED30003432 vs. Ensembl Nematostella
Match: EDO49737 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFK3])

HSP 1 Score: 428.713 bits (1101), Expect = 7.799e-138
Identity = 210/487 (43.12%), Postives = 313/487 (64.27%), Query Frame = -1
Query:    4 MRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNA 1461
            M    I +  L+    I  ++Q      TA RQ++R+R N+F A++RQ+I WFD    G+L  +LT+D+  +  G+  K+   +Q  ++F+   I+ F   WKL+ +     P+M     +M   I  ++ K+++AY+ AG IAEE+LSSIRTV AF  ++KE +RY+ +L EA+  GVK+ +  GLG G     +F   SL ++ G + + + + + G ++ +F ++++G+  LG A P +E   TA+ AAY L+ +IDR P I S  + G+ P S  GDI+F ++ F+YP+RP V +L+  + T+  G+TVA +G SG GKST+I L+QRFYDP  G +   G D++ ++L W R HIGVV QEP LF+ ++ ENI+ G+      EI K+T+ +NA++FI    Q +NT +G+RGA  SGGQKQRIAIA ALV+NP LL+LDEATSALD  SEK+VQ  LDK  +EG RT +++AHRL T+RNA
Sbjct:    1 MTDLAIYYIYLAIGALIVAYLQAGFFQYTAVRQAKRIRCNFFKAVMRQDIGWFDTYDAGELNNRLTEDISKVVDGLGSKVGLVVQFTTTFLAGFIMGFAYSWKLTLVILALTPLMVIAGGIMGKVISVFTSKELEAYAKAGAIAEEVLSSIRTVAAFGGEKKECERYNSHLGEAQAFGVKKGLSTGLGFGFFQLIMFGSYSLAFWYGAVLVADKAINSGDLLVVFFSVMVGATQLGQAGPNIEAIATARGAAYELYSIIDRQPPIDSSSEEGLKPASVKGDIDFTDIHFQYPSRPDVKVLKGLHLTIRSGQTVALVGESGCGKSTLIKLVQRFYDPAEGTVCMDGIDIRSLNLKWLRQHIGVVSQEPILFATTVAENIRYGREGITQAEIEKATKMANAHDFIRNLPQGYNTVVGERGAQMSGGQKQRIAIARALVKNPTLLILDEATSALDTESEKIVQAALDKA-SEG-RTTLVIAHRLSTIRNA 485          

HSP 2 Score: 307.76 bits (787), Expect = 1.438e-92
Identity = 173/488 (35.45%), Postives = 269/488 (55.12%), Query Frame = -1
Query:    1 IKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDK--QQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSL-MIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1446
            + F ++   +F +   Q    +++    + ++R   F +LLRQE+ +FD     TG L T L      ++     +L      +S+ V   I AF  GWKLS +    IP +    +L M  F   +  K    Y  +G IA E   ++RT+     +    + YS ++     + V+R+   G   G+    +F   +  +  G   I +G  D   V+++ + IVI  +  G    +  ++  A+ AA  +F+++DR P I S  ++G+ P +  G ++ ++V F+YPTRP V +L+  +  +  G+T+A +GPSG GKST + LL+RFYDP  GE++    +++ ++L W RS IG+V QEP LF  SI +NI  G    +    EI  + + +N + FI    + ++T +GD+G   SGGQKQRIAIA AL+RNP +LLLDEATSALD  SEK+VQ  LD   +EG RTV+M+AHRL TV+NAD
Sbjct:  595 LMFLVMGGASFFTQIFQNYMFAISGEALTVKIRRLSFKSLLRQEMAFFDDPFHTTGALTTALATHASDVKGAAGSRLGTLALGLSTVVASAIYAFYNGWKLSLVVCAFIPFIVLAGALHMKAFTGDHGGKD--DYIESGKIAVEAFENVRTIATLGREHTFFEHYSRSIDGPHKVAVRRAHLSGASYGLTEAIMFLCNAACFRFGAYLIVQGEMDMPRVMKVVMCIVIAGLVAGQISSLSPDYQKARTAAGKIFKLLDRTPAIDSASENGLQPAAVRGTVQVRSVRFKYPTRPNVKVLRGLSLEVNQGQTLALVGPSGCGKSTTVSLLERFYDPEDGEMAIDNANVRQLNLKWLRSKIGIVSQEPVLFGYSIAQNIAYGDNSREVSMAEIETAAKAANIHNFICGLPKGYDTEVGDKGTLISGGQKQRIAIARALIRNPPILLLDEATSALDTESEKVVQDALDAA-SEG-RTVIMIAHRLSTVKNAD 1078          
BLAST of SMED30003432 vs. Ensembl Nematostella
Match: EDO41930 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S348])

HSP 1 Score: 308.145 bits (788), Expect = 1.231e-94
Identity = 174/489 (35.58%), Postives = 278/489 (56.85%), Query Frame = -1
Query:    1 ISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPIL------------------QNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1413
            I+  I+    +L   R   R+R   FNA+++QE+ +FD  +TG+L+ +L+ D + I++ ++  ++  ++ I   +G ++  FT   KL+ +    IPI+         F++    K       A  +AEE + SIRTV +FS ++K +K Y+  + E+  +G + +I  G  SGV+         LV + G   + EG  + G +    L  V  ++A      +  +F+ A  A++ +F+++DRVP+I  +  GVI  SF   I F +V F YP+R    +L                  +  +F +  G+TVA +GPSG GKST+I L++RFYDP+ G I    TD+  +DL WYR  I +V QEP LF+ +I  NI  G+ DA  EEI ++ + +NA++FI   +  + T +G+RG   SGGQKQR+AIA AL+ +P++LLLDEATSALD  SE  V++ +D+ +    RTV+++AHRL TVR+A+
Sbjct:  277 IAAMIRAWLFTLAGQRLVARIRRELFNAIIKQEVAFFDTNRTGELINRLSSDTQVIQNAVTVNVSMLLRYIVQIIGSLVFMFTLSAKLTGVLISVIPIVGVGAQKYGQFVQGLRKKFQDELGNASTVAEESIGSIRTVRSFSQEKKTMKLYAEAIHESYLVGARLAIASGAFSGVVMAVGQAAIVLVLWYGGKLVNEGEMNVGILTAFALYTVNVAMAFAFLSSLYGDFMQAVGASFRMFELMDRVPKIKMEG-GVILPSFKEPIVFDDVRFSYPSREDTEVLKIEEAARQANAHDFITTFEALSFKINPGETVALVGPSGGGKSTVISLIERFYDPMTGNIRIGDTDISLLDLAWYRKKIALVSQEPVLFATTIASNIAYGR-DASQEEIEEAARQANAHDFITTFEDGYKTQVGERGVKLSGGQKQRVAIARALLMDPEILLLDEATSALDAESEHFVKEAIDRAMV--NRTVLVIAHRLSTVRSAN 761          
BLAST of SMED30003432 vs. Ensembl Nematostella
Match: EDO45926 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRD5])

HSP 1 Score: 292.352 bits (747), Expect = 2.336e-91
Identity = 166/461 (36.01%), Postives = 264/461 (57.27%), Query Frame = -1
Query:    1 TAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMD-AINEEIVKS-TQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1377
             A+R + R+ +  F +++ Q+I +FDK +TG++V++LT D   +   +S  +  F++ +    G  I  F   W+LS +  + +P++A    +  ++ KK S     A +    +A E++SS++TV +F+ ++ E+ RY     E   L +K ++  G  +G        +  ++ Y G   + EG    G ++   L  +  + AL     +    + A  AA  +F++IDR P I +    VIP S  G IEFK+V F YPTRP +P+L N +F +  G+ VA +GPSG GKST I+LL+  Y+P  GE+      +KD+D  +  + + +V QEP LF+ SI++NI  G  D A N E V+  ++ +NA+ F+    + ++T  G++G   SGGQKQRIAIA AL+RNP++LLLDEATSALD  SE LVQ  + K +     TV+++AHRL T+  AD
Sbjct:   78 VASRFNIRINNLLFGSIVNQDISFFDKTKTGEIVSRLTSDTTKMSDQVSLNVNVFLRNVFKGAGVCIFMFKLSWQLSVVTMIGLPLIAFLSEVYGNYYKKLSTAVQDAIARTNEVAAEVVSSMKTVRSFANEQGELNRYKEKNNEVFKLQIKEAVVIGGYNGCTEIMFLAMEVIILYYGGHLVLEGYLTGGNLVSFILYQLELADALEEIGDVYTGLMEAVGAAEKVFKLIDREPAILNNGK-VIPLSCEGAIEFKDVSFSYPTRPDIPVLDNVSFMVEPGQVVALVGPSGGGKSTCINLLEHLYEPTGGEVLIDAVPVKDLDHYYLHNKVSLVGQEPVLFARSIKKNIAYGVEDLADNPETVEHVSRLANAHSFVSGMPKGYDTETGEKGLQLSGGQKQRIAIARALIRNPKILLLDEATSALDAESEHLVQDAIYKNLQ--GHTVLIIAHRLSTIEKAD 535          
BLAST of SMED30003432 vs. Ensembl Nematostella
Match: EDO48846 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RHG7])

HSP 1 Score: 292.352 bits (747), Expect = 1.428e-88
Identity = 169/487 (34.70%), Postives = 260/487 (53.39%), Query Frame = -1
Query:    1 IKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDK--QQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG--KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1446
            + F  +   +F +   Q    +L+    + RLR     ALLRQEI +FD     TG L T L      ++     +L      IS+ +   I A   GWKL+      +P +A   +L I      +  Q   Y  +G +A E   ++RT+     ++   +RY+  L       V R+   G G GV    ++   +  +  G   + E       V+++ + I+I  +  G       +++ AK A   +F+++DR+P I S    G+ P +  G ++ +++ F YP RP V +L+  +  +  G+T+A +GPSG GKST + LL+RFYDP  G+++    D++ +++ W RS IG+V QEP LF  SI +NI  G    +   +EI  + + +N ++FI    + + T +GD+G   SGGQKQRIAIA ALV+NP +LLLDEATSALD  SEK+VQ  LD   +EG RTV+M+AHRL TV+NAD
Sbjct:  326 LMFLAMGGISFFTQLFQNYMFALSGEALTVRLRRMSLKALLRQEIAFFDDPMHSTGALTTALATHTSDVKGAAGSRLGTIATGISTVIASAIYASINGWKLTLAVLAFVPFLAIGRALEIKGYTGDTGGQ-NDYIESGKVAVEAFENVRTIAILGREKTFFERYAQTLVRPHRAAVVRAHLFGAGYGVTEAIMYFCFAACFRYGAHLMVEKEMTMEEVMKVVMCIMIAGMVAGELFAFSPDYLKAKVAGARIFKLVDRIPVIDSASDEGLTPSTVQGTLQMRSLRFRYPARPDVKVLRGLSLEVKQGQTLALVGPSGCGKSTTVSLLERFYDPEEGKVAVDNRDVRQLNIKWLRSKIGIVSQEPVLFGYSIAQNIAYGDNSREVSMKEIETAARAANIHDFISGLPKGYETEVGDKGTLLSGGQKQRIAIARALVKNPPILLLDEATSALDTESEKVVQDALDAA-SEG-RTVIMIAHRLSTVKNAD 809          

HSP 2 Score: 182.185 bits (461), Expect = 7.218e-49
Identity = 87/165 (52.73%), Postives = 119/165 (72.12%), Query Frame = -1
Query:    1 STIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 495
            ST+I L+QRFYDP  G +   GTD++ ++L W R  IGVV QEP LF+ +I ENI+ G+      EI ++ + +NA++FI K  + + T +G++G+  SGGQKQR+AIA ALVRNP++L+LDEATSALD  SE++VQ  LDK  A   RT +++AHRL TVRNAD
Sbjct:    1 STVIKLIQRFYDPENGSVCLDGTDIRSLNLHWLRQRIGVVSQEPALFATTIAENIRYGQDGVTQAEIEQAAKMANAHDFITKLPKGYETVVGEQGSQLSGGQKQRVAIARALVRNPRILILDEATSALDTESERVVQAALDK--AREGRTTLVIAHRLSTVRNAD 163          
BLAST of SMED30003432 vs. Ensembl Nematostella
Match: EDO39130 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SAY5])

HSP 1 Score: 257.299 bits (656), Expect = 8.587e-77
Identity = 155/478 (32.43%), Postives = 260/478 (54.39%), Query Frame = -1
Query:    1 SGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIP--ESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG------KMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1410
            + F ++  I ++  R  ++LR N F+++LRQE  +FD+ +TG+LV +L+ D   +   ++D ++  ++ ++     + +      KL+ I  + +P +A    +   F++  + +   + + A  +AEE +S+I  V AF  + +E+K Y+  ++    L  + ++ + +  G  G +   +   V Y G + +       G +    L      +++          +    A+  ++Q+ DR P +S    G+ P  +     I+FKNV+F YP+RP   I  + +  +  G   A +GPSGSGKST+  LL R YDP +G +   G D+K + L W R  +G V Q+P LFS SI ENI  G       MDAI +    + + +NA  FI      ++T +G+RG   SGGQ+QRIAIA A+++NP++LLLDEATSALD+ SE LVQ+ L++ +    RTV+ +AHRL T++NAD
Sbjct:  189 ANFGRVYLIQVSGQRIVKQLRQNLFSSILRQETAFFDRTKTGELVNRLSADTILVGKAVTDNVSDGLRAVAQVTAGVSMMAYVSPKLTGIVLLIVPPVAVGAVVYGRFLRSITKRTQDSLADATQVAEERISNIHVVRAFGQENREIKSYTDRVEYVLQLAKREALARAVFFGFTGLSGNLIVLSVLYSGGMMMTSAQISVGELTSFMLYTGFVGVSIAGLSSFYSELMKGIGASSRVWQLSDRKPAMSFT-GGLQPSIDVLARGIQFKNVNFTYPSRPNARIFADLSLDMRAGSVTAVVGPSGSGKSTLGALLLRLYDPESGSVFVGGHDVKSLSLDWLRGAVGAVHQDPILFSCSIAENIAYGAASSEVSMDAIKD----AARQANAANFIESFPNGYDTIVGERGQMLSGGQRQRIAIARAILKNPKILLLDEATSALDSESEHLVQEALERLMK--GRTVITIAHRLSTIKNAD 659          
BLAST of SMED30003432 vs. Ensembl Medaka
Match: abcb4 (ATP binding cassette subfamily B member 1 [Source:NCBI gene;Acc:101171435])

HSP 1 Score: 452.21 bits (1162), Expect = 6.113e-152
Identity = 231/555 (41.62%), Postives = 355/555 (63.96%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVS-------PRDLTMTFSERMRPS-VIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS   +FRFA G D+L++++  + +I  GA  PL  ++F D+ + L++        ++ T  F     PS  + ++I+ +   ++ ++Q+   ++ A RQ +R+R+ +F+ +++Q+I WFD  +TG+L T+LTDDV  I+ GI DK+   IQ +SSF+   ++ FT GWKL+ +     P +  + +L    +  ++ K+  AY+ AG +AEE+LSSIRTV AFS Q KE++RY  NL++A+ +G+K++I   +  G     I+   +L ++ G   I  G Y  G+V+ +F  ++IG   +G   P ++ F +A+ AA+ ++ +ID+ P I S+ + G  P+   GDIEF N+ F YP+RP V IL+N + ++  G+T+A +G SG GKST I LLQRFYDP+ G +S  G D++ +++ + R  IGVV QEP LF+ +I ENI+ G+ D    EI ++ +++NAY+FIM    KF T +GDRG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK      RT ++VAHRL T+RNAD
Sbjct:   29 VSPIAVFRFADGWDILMLVIGTVMAIANGAVLPLMCIVFGDMTDSLINGMKGCFRAKNATSLF-----PSFAVYYSIMGAVVLVAAYLQVSLWTIAAGRQVKRIRTLFFHKIMQQDIGWFDINETGELNTRLTDDVYKIQEGIGDKVGMLIQSVSSFITAFVIGFTKGWKLTLVILAVSPALGISGALFSKVLTSFTSKEQSAYAKAGAVAEEVLSSIRTVFAFSGQNKEIERYHKNLQQAKKMGMKKAISANIAMGFTFLMIYFSYALAFWYGSTLILSGEYTIGSVLTVFFVVIIGVFTMGQTSPNIQTFASARGAAHKVYNIIDKEPIIDSYSEDGFKPDFIKGDIEFSNIHFTYPSRPDVKILENMSLSVKSGQTMALVGSSGCGKSTTIQLLQRFYDPVEGNVSIDGHDIRSLNVRYLREMIGVVSQEPILFATTISENIRYGRPDVTQPEIEQAAKEANAYDFIMNLPNKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQSALDKV--RLGRTTLIVAHRLSTIRNAD 576          
BLAST of SMED30003432 vs. Ensembl Medaka
Match: abcb4 (ATP binding cassette subfamily B member 1 [Source:NCBI gene;Acc:101171435])

HSP 1 Score: 455.292 bits (1170), Expect = 4.218e-146
Identity = 233/561 (41.53%), Postives = 358/561 (63.81%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLV--SPRDLT-----MTF-------SERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQI-SHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            VS   +FRFA G D+L++++  + +I  GA  PL  ++F D+ + L+  +P ++T     +TF        E M    + ++I+ +   ++ ++Q+   ++ A RQ +R+R+ +F+ +++Q+I WFD  +TG+L T+LTDDV  I+ GI DK+   IQ +SSF+   ++ FT GWKL+ +     P +  + +L    +  ++ K+  AY+ AG +AEE+LSSIRTV AFS Q KE++RY  NL++A+ +G+K++I   +  G     I+   +L ++ G   I  G Y  G+V+ +F  ++IG   +G   P ++ F +A+ AA+ ++ +ID+ P I S+ + G  P+   GDIEF N+ F YP+RP V IL+N + ++  G+T+A +G SG GKST I LLQRFYDP+ G +S  G D++ +++ + R  IGVV QEP LF+ +I ENI+ G+ D    EI ++ +++NAY+FIM    KF T +GDRG   SGGQKQRIAIA ALVRNP++LLLDEATSALD  SE +VQ  LDK      RT ++VAHRL T+RNAD
Sbjct:   41 VSPIAVFRFADGWDILMLVIGTVMAIANGAVLPLMCIVFGDMTDSLINGAPPNMTAGYPNLTFINMTIDIEEEMTVFAVYYSIMGAVVLVAAYLQVSLWTIAAGRQVKRIRTLFFHKIMQQDIGWFDINETGELNTRLTDDVYKIQEGIGDKVGMLIQSVSSFITAFVIGFTKGWKLTLVILAVSPALGISGALFSKVLTSFTSKEQSAYAKAGAVAEEVLSSIRTVFAFSGQNKEIERYHKNLQQAKKMGMKKAISANIAMGFTFLMIYFSYALAFWYGSTLILSGEYTIGSVLTVFFVVIIGVFTMGQTSPNIQTFASARGAAHKVYNIIDKEPIIDSYSEDGFKPDFIKGDIEFSNIHFTYPSRPDVKILENMSLSVKSGQTMALVGSSGCGKSTTIQLLQRFYDPVEGNVSIDGHDIRSLNVRYLREMIGVVSQEPILFATTISENIRYGRPDVTQPEIEQAAKEANAYDFIMNLPNKFETLVGDRGTQMSGGQKQRIAIARALVRNPKILLLDEATSALDAESETIVQSALDKV--RLGRTTLIVAHRLSTIRNAD 599          

HSP 2 Score: 323.553 bits (828), Expect = 3.807e-97
Identity = 189/534 (35.39%), Postives = 287/534 (53.75%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFD--KQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYY-------MGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISH-KKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIV------KSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G   P+  ++F  ++ +   P    +   ER +   + F  +   +F++ F+Q      +    + +LR   F A+LRQ++ WFD  K   G L T+L  D   ++     +LA   Q  ++    IILAF  GW+L+ +    +PI+A   ++ +  +  ++ +  K    AG IA E + +IRTV + + + K    Y  NLK       K++   G       FT     S++Y+        G   I +G  D   V  +   ++ G++A+G A     N+  AK +A  L  +I++VP I +    G  P+ F G++ FK+V F YP+RP VPIL+  N  +  G+T+A +G SG GKST I LL+RFYD   G++     D+K +++ W RS IG+V QEP LF  ++ ENI  G     N   V       + + +N ++FI +  Q++NT  GD+G   SGGQKQRIAIA A++RNPQ+LLLDEATSALD  SEK+VQ  LD+  A   RT ++VAHRL T+RNAD
Sbjct:  729 GGIQPVFAVLFSKIIAVFAEPDQEVV--RERSQFFSLMFVTIGCVSFVTMFLQGFCFGKSGEVLTLKLRLGAFKAMLRQDLGWFDDSKNSVGALTTRLATDAAQVQGATGARLATVAQNFANLGTGIILAFIYGWELTLLVLAVVPIIAVAGAVGMQMLTGHAAEDKKELEKAGKIATEAIENIRTVASLTREAKFESLYQENLKVPYKNSQKKAHTHG-------FTFSFSQSMIYFAYAGCFRFGAWLIVQGRMDVEGVFLVISAVLFGAMAVGEANSFAPNYAKAKMSAAHLMMLINKVPDIDNLSNEGETPDKFNGNVIFKDVRFNYPSRPDVPILKGLNLKVKKGETLALVGSSGCGKSTTIQLLERFYDTKEGKVVLDDVDIKQLNIHWLRSQIGIVSQEPVLFDCTLAENIAYGD----NSRTVTMEEIEAAAKAANIHKFIEELPQRYNTQAGDKGTQLSGGQKQRIAIARAILRNPQVLLLDEATSALDTESEKVVQDALDQ--ASKGRTCIIVAHRLSTIRNAD 1247          
BLAST of SMED30003432 vs. Ensembl Medaka
Match: abcb11a (ATP binding cassette subfamily B member 11 [Source:NCBI gene;Acc:101157171])

HSP 1 Score: 404.831 bits (1039), Expect = 5.592e-127
Identity = 234/583 (40.14%), Postives = 351/583 (60.21%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLV------------------------------SPRDLTMTFSERMRPSVIKFA-----ILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMG-LLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISH-KKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V Y+QL+RFATG D ++++V    ++  GAA PL +L++  + +  V                              +  + T+T    +   + +FA     I S    +S F  M  +S  AARQ++R+R  YF  +++ EI WFD    G+L T+++DD+  I + I+D+++ FI++IS+F+   ++ F  GWKL+ +     P++     LM   + + + +++KAY+ AG +A+E+LSSIRTV AF  +EKE +RY  NL EA+  GVK+    G+  G +   IF   +L ++ G  L I      PG +IQ+F  ++I +  LG A P LE F + +AAA  +F+ IDR P+I    + G   +   GDIEF NV F YP+RP + IL + N  +  G+T AF+GPSGSGK++ + L+QRFYDP  G ++  G DL+ +++ W RS IG+V+QEP LF+ +I ENI+ G+     E+I+++T+++NAY FIM   QKF+T +G+ G   SGGQKQRIAIA ALVR P++LLLD ATSALDN SE  VQ+ L+K   E  RT + VAHRL T+R+AD
Sbjct:   28 VGYFQLYRFATGRDTVMMVVGGFCALIHGAATPLMLLVYGMMTDTFVAYELEVQELKDPNKECLNDTIYWINGSIYETTDNQTVTCGVNIEGQMTQFAYYYIGIGSGVLLVSYFQIMFWVS-AAARQTQRIRKTYFRRVMQMEIGWFDCNSVGELNTRISDDINKISNAIADQVSIFIERISTFIFGFMVGFIGGWKLTLVVVAVSPLIGMGAGLMAMAVARLTGRELKAYAKAGAVADEVLSSIRTVAAFGGEEKEAERYDRNLVEAQNWGVKKGSIIGVFQGYLWCIIFLCYALAFWYGSKLVIDSKELSPGNLIQVFFGVLIAATNLGQAAPCLEAFASGRAAAKTVFETIDREPEIDCLSEDGYKLDKIKGDIEFHNVTFYYPSRPDIKILDDLNLHIKAGETTAFVGPSGSGKTSAVQLIQRFYDPKEGMVTLDGHDLRSLNIQWLRSLIGIVEQEPVLFATTIAENIRYGRPGVTMEDIIQATREANAYHFIMDLPQKFDTLVGEGGGQMSGGQKQRIAIARALVRKPRILLLDMATSALDNESEATVQEALNKVHTE--RTTISVAHRLSTIRSAD 607          

HSP 2 Score: 293.123 bits (749), Expect = 3.682e-86
Identity = 183/522 (35.06%), Postives = 285/522 (54.60%), Query Frame = -1
Query:    1 GAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLG-KMDAIN-EEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            G+  P+  ++F  ++     P DL      ++    + F +++  +F S FIQ  + + +    +RRLR   F A+L+QEI WFD  +   G L T+L  D   ++     ++   I  ++S     I+AF   WKL+ +    +P++  +       +  ++ +  KA   AG ++ E L +IRTV   + +   V+ +   L+       KR+   GL  G+    IF   +  +  G   +         V ++   +VI   ALG A     ++  AK AA   F+++DRVP+ISH   G   E+F G++EF N  F YPTRP   +L+    ++  G+T+A +G SG GKST + LL+RFYDP  G++   G     + + + RS IG+V QEP LF  SI ENIQ G     IN EEIV++ + +N ++F+M    K++T +G +G+  S GQKQRIAIA A++RNP++LLLDEATSALD  SE++VQ  LD+  A   RT +++AHRL T++NAD
Sbjct:  753 GSVNPIYAVLFSQILGTFSIP-DLDEQ-RRQINGICLLFCVVAVISFFSQFIQGFSFAKSGELLTRRLRKVGFQAMLKQEIGWFDDPENSPGALTTRLATDASMVQGATGSQIGMIINSLTSIGASFIIAFYFSWKLTLVILGFLPLIGLSGVFQAKMLTGFAKEDKKAMEAAGRVSSEALGNIRTVAGLTKERSFVESFEEKLELPYKSAKKRANIYGLCFGLTQCVIFMAYAASFRFGGYLVRAEGLQYMLVFRVISAVVISGTALGRASSFTPDYAKAKTAAAQFFKLLDRVPKISHTD-GEKWENFKGEVEFLNCKFTYPTRPDTQVLKGLVVSVKPGQTLALVGSSGCGKSTGVQLLERFYDPDEGKVLIDGRPSYSVSVPFLRSQIGIVSQEPVLFDCSIAENIQYGDNSHRINMEEIVEAAKTANLHDFVMTLPDKYDTQVGAQGSQLSRGQKQRIAIARAIIRNPKILLLDEATSALDTESEQIVQSALDE--ARKGRTCIVIAHRLSTIQNAD 1269          
BLAST of SMED30003432 vs. Ensembl Medaka
Match: abcb11b (bile salt export pump [Source:NCBI gene;Acc:101163554])

HSP 1 Score: 398.282 bits (1022), Expect = 3.846e-124
Identity = 233/604 (38.58%), Postives = 354/604 (58.61%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIF---------------------RDVVNLLVSPR-------DLTM----TFSERMRPSVIKFAILS--SC----------------------TFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMG-LLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISH-KKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V ++QLFRFAT  D+L++++  + ++  G+A PL +L+F                     ++ VN  +  +       D+T+    +F   +  ++  F  LS  SC                       F+ G+ Q+      AARQ + +R  YF  ++R EI WFD    G+L T+L+DD+  I   I+D+++ F+Q+ ++FV    + F  GWKL+ +   + P++     LM  F+ K +  +++AY+ AG +A+E+LSSIRTV AF  + KEV+RY  NL  A+  G+++ +  G  +G +   IF   +L ++ G  L +    Y PGT++Q+F  +++ +++LG A P LE F   + AA  +F+ IDR PQI    + G   E   GDIEF NV F YP+RP V IL   +  +  G+T AF+GPSG+GKST I L+QRFYDPL G ++  G D++ +++ W RS IG+V+QEP LF+ +I ENI+ G+     ++I+ + +++NAY FIM   QKF+T +G+ G   SGGQKQRIAIA ALVRNP++LLLD ATSALDN SE +VQ+ LDK      RT + +AHRL T++NAD
Sbjct:   55 VGFFQLFRFATCKDVLMMVLGSVCAVLHGSAQPLMLLVFGLLTDTFIDYDIELQELSDDRKECVNNTIQWKRNYTGTLDMTLPLNQSFGSLINSTLEMFTPLSNMSCGILDIEHEMTLFALYYVGIGAGVFLLGYFQISLWVTAAARQIQLIRKLYFTKVMRMEIGWFDCTSVGELNTRLSDDINKINDAIADQVSIFVQRFTTFVCGFCIGFVKGWKLTLVIVAASPLIGIGAGLMALFVAKLTGMELQAYAKAGAVADEVLSSIRTVAAFGGEIKEVERYDRNLVSAQRWGIRKGLIMGFFTGYMWLIIFLCYALAFWYGSTLVVDTAEYTPGTLLQVFFGVLVAAMSLGQASPCLEAFAAGRGAATIIFETIDREPQIDCLSESGYKLERVKGDIEFHNVTFHYPSRPEVKILDQLSVQVKSGETTAFVGPSGAGKSTAIQLIQRFYDPLEGMVTLDGHDIRGLNIQWLRSLIGIVEQEPVLFATTIAENIRYGRPGVSMDDIINAAKEANAYNFIMDLPQKFDTLVGEGGGQMSGGQKQRIAIARALVRNPRILLLDMATSALDNESEAVVQEALDKV--RMGRTTISIAHRLSTIKNAD 656          

HSP 2 Score: 280.411 bits (716), Expect = 1.661e-81
Identity = 164/485 (33.81%), Postives = 267/485 (55.05%), Query Frame = -1
Query:    1 FAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQIS-HKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGK--MDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1440
            FA++   +F +  +Q    S +    +RRLR   F+A+L QEI WFD  +   G L T+L  D   ++     ++   +  +++    ++++F   WKL+ +    +P +A +       +  ++ +  +A   AG I+ E L++IRT+     ++  V+ Y   L       +K++   G   G     +F   S  Y  G   + +       V ++   IV    ALG A     ++  AK +A   FQ++DR+P IS +   G    +F G+IEF +  F YPTRP + +L     ++  G+T+AF+G SG GKST + LL+RFYDP  G++   G D K +++ + RS IG+V QEP LF  SI ENI+ G    +   +E++ + + +  ++F+    +++NT +G +G+  S GQKQRIAIA A++R+P++LLLDEATSALD  SEK VQ+ LDK  A   RT +++AHRL T++N+D
Sbjct:  844 FAMVGVVSFFTQMLQGYAFSKSGELLTRRLRRIGFHAMLGQEIGWFDDHRNSPGALTTRLATDASQVQGATGSQIGMIVNSLTNIGVAVLMSFYFSWKLTLLILCFLPFLALSGGFQAKMLTGFAKQDKQAMETAGRISGEALNNIRTIAGLGKEKNFVEMYEFQLDAPYQAALKKANVYGACYGFAQCVVFMTNSASYRFGGYLVKQEGLHFSLVFRVISAIVTSGTALGRASSYTPDYAKAKISAARFFQLLDRIPTISVYSDKGDKWNNFQGNIEFIDCKFTYPTRPDIQVLNGLTVSVKPGQTLAFVGSSGCGKSTSVQLLERFYDPDHGKVLIDGHDSKHVNVPYLRSKIGIVSQEPILFDCSIAENIKYGDNSREISMDEVILAAKKAQLHDFVTALPEQYNTNVGSQGSQLSRGQKQRIAIARAIIRDPKILLLDEATSALDTESEKTVQEALDK--AREGRTCIVIAHRLSTIQNSD 1326          
BLAST of SMED30003432 vs. Ensembl Medaka
Match: abcb8 (ATP binding cassette subfamily B member 8 [Source:NCBI gene;Acc:101174865])

HSP 1 Score: 322.013 bits (824), Expect = 1.095e-100
Identity = 189/530 (35.66%), Postives = 304/530 (57.36%), Query Frame = -1
Query:    1 GAAFPLNV---LIFRDVVNLLVSP-RDLTMTFSERMRPSVIKFAILSSCT--FISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEA----ETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPE-SFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSH-IGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1554
            GAA  LN+   L+   +VN++    RD T  +   MR   +K   +        SG+I  + +S    R +  +R   F +LLRQ++ +FD  +TGQLV +LT D++  +S     ++  ++ ++  VGC +  +    KL+ +  + +P +    +L+  F++K S    +  + A G+A+E L ++RTV AF+ +E+E++ Y++ + ++    E LG   +I QGL +  +      +G++  + G   I      PG ++   +       +L +   +    +   ++   +F+ +   P I     G IP  S  G ++F N+ F YPTRPG  +L+ FN  LP  KT+A +G SG GKST+  LL+RFYDP +G I   G D++ +DL+W R   IG + QEP LF  SI ENI+ GK +A + E++++++ +NAY FI    + FNT +G+RGA  SGGQKQRIAIA AL++NP +L+LDEATSALD  SE++VQ+ LD+  A   RTV+++AHRL T++ AD
Sbjct:  146 GAAI-LNIQIPLMLGHLVNVVARHLRDNTGNYIGEMRGPALKLLGMYGIQGLLTSGYI--ILLSRVGERVAADMRKTLFASLLRQDVAFFDANKTGQLVNRLTADIQEFKSSFKLVISQGLRSVTQTVGCFVSLYVISPKLTGLTVVVLPCLVGAGALIGSFLRKLSRLAQEQVAKATGVADEALGNVRTVKAFAMEERELQLYAYEVDKSCEMNENLGNGIAIFQGLSN--VALNCIVLGTI--FAGGTLISNNELSPGDLMSFLVASQTVQRSLASISILFGQMVRGISSGARVFEYLSLQPSIPLCGGGRIPHHSLMGRVDFMNISFSYPTRPGHEVLKKFNLMLPPSKTIAIVGESGGGKSTVAALLERFYDPSSGVIMLDGLDIRTLDLSWLRGQVIGFINQEPVLFGSSIMENIRFGKPEATDAEVIEASKQANAYGFITSFPEGFNTLVGERGATLSGGQKQRIAIARALIKNPSILVLDEATSALDAESERVVQEALDR--ATRGRTVLIIAHRLSTIQRAD 666          
BLAST of SMED30003432 vs. Planmine SMEST
Match: SMESG000027392.1 (SMESG000027392.1)

HSP 1 Score: 1143.26 bits (2956), Expect = 0.000e+0
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = -1
Query:    1 MGKTSYNVEEANFANSILKEKPAKSKTDIXXXXXXXXXXXXXXSVSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1770
            MGKTSYNVEEANFANSILKEKPAKSKTDIPEAKKEKPKKSAMESVSYYQLFRFATGIDLLIVIVMIISSICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD
Sbjct:    1 MGKTSYNVEEANFANSILKEKPAKSKTDIPEAKKEKPKKSAMESVSYYQLFRFATGIDLLIVIVMIISSICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 590          

HSP 2 Score: 285.034 bits (728), Expect = 1.787e-83
Identity = 184/541 (34.01%), Postives = 297/541 (54.90%), Query Frame = -1
Query:    1 VGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMV-------TISLTAARQSRRLRSNYFNALLRQEICWFDKQQT--GQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEIL-----SSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPES-FYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLA------GEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAIN-EEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1557
            VGA FP   LI+ ++ N+      L  TF + M   + K   L     I GFI +         +       + RLR+   +++L+QE+ +FD+ Q   G L  +L  +   ++S    +L+ FI+ I +    + + F   W+LS +  + IP +A T       I +  +   +  S AG ++   L     ++IRTV + S Q+    +Y  +LK        + +  G  SG+    ++ V  +V+++G +F+ +G  D  +++++F    + + ALG A+ + ++   A+ AA    Q IDR+  I   K GV+PE    G+IEFKN+ F YPTR  V +L+  +  +  G+TVA +G SG GKST+I LL+R+YDP        G++   G D+K ++L+W R+ IG+V+QEP LF+ S+ +NI  G    ++ E ++K  + +N ++FI+   + +NT +G RG   SGGQKQRI IA AL   P +LLLDEATSALD  SE +VQ+ LD+  A+   T +++AHRL T++NAD
Sbjct:  699 VGAVFPCASLIYAELYNVFA----LKFTFPDLM---ISKGRTLVLLHLIPGFILLFGQLTKAWCLGKAGEALTTRLRTFMMSSVLKQEMAYFDEPQNLPGVLTARLAVEASLVKSAAGIQLSVFIEAIVNLSASLFIGFYYNWELSLLFLIFIPCIALTG------IMQGKLSTGQRLSTAGSVSSSSLVLEVLNNIRTVNSLSLQDHFGDKYFDSLKLDGKKIKSKLVFLGCVSGLAVSALYYVYCVVFFVGAIFLDKGKTDMISLMRVFSCFTMSTAALGRALSLAQDTEKARKAARKTLQTIDRLTAIDAHK-GVVPEEKLTGNIEFKNLHFRYPTRANVSVLRGLDLKINAGQTVALVGHSGCGKSTLIQLLERYYDPWVSKNYGQGQVLLDGHDIKALNLSWVRNQIGLVKQEPSLFNISVADNIIYGSNSDVDYEHMIKVAKMANIHDFILSLPEGYNTVVGQRGGHLSGGQKQRICIARALYNKPPILLLDEATSALDTQSEIVVQEALDR--AQENTTCLVIAHRLSTIKNAD 1223          
BLAST of SMED30003432 vs. Planmine SMEST
Match: SMESG000027395.1 (SMESG000027395.1)

HSP 1 Score: 764.607 bits (1973), Expect = 0.000e+0
Identity = 369/546 (67.58%), Postives = 455/546 (83.33%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V +++LFRF   +D+L++ + +ISSI +G A PLN+LIF+DVVNLLV P    + FS  +R  +  FAIL+S   I+ F+Q + IS+T  RQSRR +SNYF ALLRQEI WFDKQQTGQ++T+ +DD++SIE  ISDKLA FIQQ+S F   II+A T+GWKL+ +G M+IPIM       +HFIKKY I QI+AYS+AGGIAEEILSS+RTVVAF+AQE EV RYS NL +A++LG+K+SI QG+GSG +G ++F++GSLV+Y+GLL I   +YDPGTVIQ+FLTIV+GSI++G A P++ENF+TA+AAAY +F+VIDRVP I H+K G+IP++F  DI+F+NV F+Y TRPG+ IL NFNFT+PYGKTVAFIGPSGSGKSTIIHLLQRFYDPL+GE+     ++KDIDL WYRSHIGVVQQEPPLFSGSI+ENIQ+GK+DA  +EI+KSTQ SNAY+FIM TK+ F+T LGDRG GFSGGQKQRIAIA AL+R+PQLLLLDEATSALDN SEKLVQKTLD TIAEGKRT+VMVAHRLQTVRNAD
Sbjct:   50 VPFHRLFRFMNSMDILLISLTVISSIGIGVALPLNMLIFKDVVNLLVLPTG-NINFSSAIRNPINYFAILASGALIASFVQSICISVTGKRQSRRYKSNYFAALLRQEISWFDKQQTGQMITRFSDDIDSIEVAISDKLAQFIQQLSLFFSSIIIAATSGWKLTLVGCMAIPIMIIILYSNLHFIKKYVIAQIRAYSVAGGIAEEILSSVRTVVAFNAQESEVNRYSKNLVKAKSLGIKKSIVQGIGSGCVGLSVFSIGSLVFYVGLLLINSENYDPGTVIQVFLTIVLGSISIGYAAPLIENFMTAQAAAYSIFEVIDRVPSIDHRKLGIIPDTFAKDIKFQNVTFQYSTRPGMTILDNFNFTIPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLSGEVILGDQNVKDIDLNWYRSHIGVVQQEPPLFSGSIRENIQMGKLDATEDEIIKSTQYSNAYDFIMATKETFDTKLGDRGTGFSGGQKQRIAIATALIRDPQLLLLDEATSALDNKSEKLVQKTLDTTIAEGKRTIVMVAHRLQTVRNAD 594          
BLAST of SMED30003432 vs. Planmine SMEST
Match: SMESG000027395.1 (SMESG000027395.1)

HSP 1 Score: 764.222 bits (1972), Expect = 0.000e+0
Identity = 369/546 (67.58%), Postives = 455/546 (83.33%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V +++LFRF   +D+L++ + +ISSI +G A PLN+LIF+DVVNLLV P    + FS  +R  +  FAIL+S   I+ F+Q + IS+T  RQSRR +SNYF ALLRQEI WFDKQQTGQ++T+ +DD++SIE  ISDKLA FIQQ+S F   II+A T+GWKL+ +G M+IPIM       +HFIKKY I QI+AYS+AGGIAEEILSS+RTVVAF+AQE EV RYS NL +A++LG+K+SI QG+GSG +G ++F++GSLV+Y+GLL I   +YDPGTVIQ+FLTIV+GSI++G A P++ENF+TA+AAAY +F+VIDRVP I H+K G+IP++F  DI+F+NV F+Y TRPG+ IL NFNFT+PYGKTVAFIGPSGSGKSTIIHLLQRFYDPL+GE+     ++KDIDL WYRSHIGVVQQEPPLFSGSI+ENIQ+GK+DA  +EI+KSTQ SNAY+FIM TK+ F+T LGDRG GFSGGQKQRIAIA AL+R+PQLLLLDEATSALDN SEKLVQKTLD TIAEGKRT+VMVAHRLQTVRNAD
Sbjct:   50 VPFHRLFRFMNSMDILLISLTVISSIGIGVALPLNMLIFKDVVNLLVLPTG-NINFSSAIRNPINYFAILASGALIASFVQSICISVTGKRQSRRYKSNYFAALLRQEISWFDKQQTGQMITRFSDDIDSIEVAISDKLAQFIQQLSLFFSSIIIAATSGWKLTLVGCMAIPIMIIILYSNLHFIKKYVIAQIRAYSVAGGIAEEILSSVRTVVAFNAQESEVNRYSKNLVKAKSLGIKKSIVQGIGSGCVGLSVFSIGSLVFYVGLLLINSENYDPGTVIQVFLTIVLGSISIGYAAPLIENFMTAQAAAYSIFEVIDRVPSIDHRKLGIIPDTFAKDIKFQNVTFQYSTRPGMTILDNFNFTIPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLSGEVILGDQNVKDIDLNWYRSHIGVVQQEPPLFSGSIRENIQMGKLDATEDEIIKSTQYSNAYDFIMATKETFDTKLGDRGTGFSGGQKQRIAIATALIRDPQLLLLDEATSALDNKSEKLVQKTLDTTIAEGKRTIVMVAHRLQTVRNAD 594          
BLAST of SMED30003432 vs. Planmine SMEST
Match: SMESG000027395.1 (SMESG000027395.1)

HSP 1 Score: 751.125 bits (1938), Expect = 0.000e+0
Identity = 365/546 (66.85%), Postives = 451/546 (82.60%), Query Frame = -1
Query:    1 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAINEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALVRNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRNAD 1638
            V +++LFRF   +D+L++ + +ISSI +G A PLN+LIF+DVVNLLV P    + FS  +R  +  FAIL+S   I+ F+Q + IS+T  RQSRR +SNYF ALLRQEI WFDKQQTGQ++T+ +DD++SIE  ISDKLA FIQQ+S F   II+A T+GWKL+ +G M+IPIM       +HFIKKY I QI+AYS+AGGIAEEILSS+RTVVAF+AQE EV RYS NL +A++LG+K+SI QG+GSG +G ++F++GSLV+Y+GLL I   +YDPGTVIQ+FLTIV+GSI++G A P++ENF+TA+AAAY +F+V    P I H+K G+IP++F  DI+F+NV F+Y TRPG+ IL NFNFT+PYGKTVAFIGPSGSGKSTIIHLLQRFYDPL+GE+     ++KDIDL WYRSHIGVVQQEPPLFSGSI+ENIQ+GK+DA  +EI+KSTQ SNAY+FIM TK+ F+T LGDRG GFSGGQKQRIAIA AL+R+PQLLLLDEATSALDN SEKLVQKTLD TIAEGKRT+VMVAHRLQTVRNAD
Sbjct:   50 VPFHRLFRFMNSMDILLISLTVISSIGIGVALPLNMLIFKDVVNLLVLPTG-NINFSSAIRNPINYFAILASGALIASFVQSICISVTGKRQSRRYKSNYFAALLRQEISWFDKQQTGQMITRFSDDIDSIEVAISDKLAQFIQQLSLFFSSIIIAATSGWKLTLVGCMAIPIMIIILYSNLHFIKKYVIAQIRAYSVAGGIAEEILSSVRTVVAFNAQESEVNRYSKNLVKAKSLGIKKSIVQGIGSGCVGLSVFSIGSLVFYVGLLLINSENYDPGTVIQVFLTIVLGSISIGYAAPLIENFMTAQAAAYSIFEV----PSIDHRKLGIIPDTFAKDIKFQNVTFQYSTRPGMTILDNFNFTIPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLSGEVILGDQNVKDIDLNWYRSHIGVVQQEPPLFSGSIRENIQMGKLDATEDEIIKSTQYSNAYDFIMATKETFDTKLGDRGTGFSGGQKQRIAIATALIRDPQLLLLDEATSALDNKSEKLVQKTLDTTIAEGKRTIVMVAHRLQTVRNAD 590          
BLAST of SMED30003432 vs. Planmine SMEST
Match: SMESG000027399.1 (SMESG000027399.1)

HSP 1 Score: 531.176 bits (1367), Expect = 0.000e+0
Identity = 258/399 (64.66%), Postives = 328/399 (82.21%), Query Frame = -1
Query:  442 VSYYQLFRFATGXXXXXXXXXXXXXICVGAAFPLNVLIFRDVVNLLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSRRLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQQISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIKAYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQGLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIALGNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKNVDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLAGEI 1638
            V +++LFRF   +D+ ++++M+ISS+ +G A PLNVLIF+DVVNLLV P    + F   M+  +I FAIL+S   I+ F+Q + IS+T  RQSRR +SNYF ALLRQEI WFDKQQTGQ++T+ +DD++SIE  ISDKLA FIQQIS F+G II++   GWKL+ +G +SIPIM   F  MI+FIKKY I QI+AYS+AGGIAEEILSS+RTVVAF+AQE EV RYS NL +A++LG+K+SI QG+GSG +G ++F++GSLV+Y+GLL I   +YDPGTVIQ+FLTIV+GSI+LG AVP++ENF+TA+AAAY +F+VIDRVP I HKK G+IP++F  DI+ +NV FEYPTRPGV IL NFNFT+PYGKTVAFIGPSGSGKSTIIHLLQRFYDPL+GE+
Sbjct:   70 VPFHRLFRFMDSMDVFLIVLMVISSMGLGVALPLNVLIFKDVVNLLVLPPG-NIKFVSAMQNLIIYFAILASGALIASFVQSICISVTGKRQSRRYKSNYFAALLRQEISWFDKQQTGQMITRFSDDIDSIEVAISDKLAQFIQQISLFIGSIIISAINGWKLTLVGCVSIPIMIIVFYFMINFIKKYVIAQIRAYSVAGGIAEEILSSVRTVVAFNAQESEVNRYSKNLVKAKSLGIKKSIVQGIGSGCVGLSVFSIGSLVFYVGLLLINSENYDPGTVIQVFLTIVLGSISLGIAVPIIENFMTAQAAAYSIFEVIDRVPTIDHKKAGIIPDTFASDIKLQNVTFEYPTRPGVTILDNFNFTIPYGKTVAFIGPSGSGKSTIIHLLQRFYDPLSGEV 467          
The following BLAST results are available for this feature:
BLAST of SMED30003432 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ABCB18.751e-14440.53ATP binding cassette subfamily B member 1 [Source:... [more]
ABCB18.751e-14440.53ATP binding cassette subfamily B member 1 [Source:... [more]
ABCB57.333e-14041.64ATP binding cassette subfamily B member 5 [Source:... [more]
ABCB41.769e-13240.65ATP binding cassette subfamily B member 4 [Source:... [more]
ABCB41.769e-13240.65ATP binding cassette subfamily B member 4 [Source:... [more]
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BLAST of SMED30003432 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pgp-92.844e-12639.57p-GlycoProtein related [Source:UniProtKB/TrEMBL;A... [more]
pgp-12.424e-12139.25Multidrug resistance protein pgp-1 [Source:UniPro... [more]
pgp-24.938e-11735.88ABC transporter PGP-2; p-GlycoProtein related [So... [more]
pgp-25.628e-11735.88ABC transporter PGP-2; p-GlycoProtein related [So... [more]
pgp-26.183e-11735.88ABC transporter PGP-2; p-GlycoProtein related [So... [more]
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BLAST of SMED30003432 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mdr501.370e-12538.11gene:FBgn0010241 transcript:FBtr0087537[more]
Mdr491.648e-11136.55gene:FBgn0004512 transcript:FBtr0087801[more]
Mdr658.816e-10538.16gene:FBgn0004513 transcript:FBtr0077011[more]
CG102264.057e-10133.90gene:FBgn0035695 transcript:FBtr0077012[more]
CG102262.003e-10038.23gene:FBgn0035695 transcript:FBtr0332068[more]
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BLAST of SMED30003432 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
abcb41.168e-14442.07ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
abcb51.188e-13939.40ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
abcb51.297e-13939.24ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
abcb11a1.051e-12738.49ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
abcb11b5.795e-12443.46ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
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BLAST of SMED30003432 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mia21.381e-15143.01MIA SH3 domain ER export factor 2 [Source:Xenbase;... [more]
mia21.460e-15143.01MIA SH3 domain ER export factor 2 [Source:Xenbase;... [more]
mia24.276e-15142.86MIA SH3 domain ER export factor 2 [Source:Xenbase;... [more]
mia26.877e-15142.91MIA SH3 domain ER export factor 2 [Source:Xenbase;... [more]
mia21.426e-15043.76MIA SH3 domain ER export factor 2 [Source:Xenbase;... [more]
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BLAST of SMED30003432 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Abcb1a4.764e-14441.64ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
Abcb1b1.228e-13943.00ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
Abcb41.822e-13540.90ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
Abcb53.510e-13240.25ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
Abcb41.144e-13140.90ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
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BLAST of SMED30003432 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P21447|MDR1A_MOUSE3.332e-14341.64ATP-dependent translocase ABCB1 OS=Mus musculus OX... [more]
sp|P21448|MDR1_CRIGR3.439e-14341.39ATP-dependent translocase ABCB1 OS=Cricetulus gris... [more]
sp|P08183|MDR1_HUMAN4.203e-14340.53ATP-dependent translocase ABCB1 OS=Homo sapiens OX... [more]
sp|P43245|MDR1_RAT2.574e-13940.81ATP-dependent translocase ABCB1 OS=Rattus norvegic... [more]
sp|Q2M3G0|ABCB5_HUMAN3.522e-13941.64ATP-binding cassette sub-family B member 5 OS=Homo... [more]
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BLAST of SMED30003432 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4S2MG492.538e-16846.91Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4V6RH953.713e-16846.91Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4E0RLC95.996e-16546.62Multidrug resistance protein 1 OS=Fasciola hepatic... [more]
A0A504YDC31.476e-16246.25Multidrug resistance protein 1 OS=Fasciola giganti... [more]
A0A4E0RE861.558e-16245.62ATP binding cassette subfamily B MDR TAP OS=Fascio... [more]
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BLAST of SMED30003432 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
abcb52.530e-14040.00ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
abcb58.789e-13543.48ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
abcb53.325e-13240.15ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
abcb11a8.401e-13240.78ATP binding cassette subfamily B member 11 [Source... [more]
abcb11b1.015e-12938.87bile salt export pump-like [Source:NCBI gene;Acc:1... [more]
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BLAST of SMED30003432 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
abcb11b4.181e-12439.38ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
ABCB14.306e-9543.86ATP binding cassette subfamily B member 1 [Source:... [more]
abcb84.395e-9335.70ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
ENSPMAT00000001786.13.872e-9236.36pep scaffold:Pmarinus_7.0:GL477870:201625:214977:-... [more]
abcb93.135e-8132.30ATP-binding cassette, sub-family B (MDR/TAP), memb... [more]
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BLAST of SMED30003432 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
MDL17.190e-7233.68Mitochondrial inner membrane half-type ABC transpo... [more]
MDL24.228e-6933.54Mitochondrial inner membrane half-type ABC transpo... [more]
ATM18.555e-5831.73Mitochondrial inner membrane ATP-binding cassette ... [more]
STE67.387e-4925.54Plasma membrane ATP-binding cassette (ABC) transpo... [more]
YOR11.051e-3325.14Plasma membrane ATP-binding cassette (ABC) transpo... [more]
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BLAST of SMED30003432 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO497377.799e-13843.12Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO419301.231e-9435.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO459262.336e-9136.01Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO488461.428e-8834.70Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO391308.587e-7732.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30003432 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
abcb46.113e-15241.62ATP binding cassette subfamily B member 1 [Source:... [more]
abcb44.218e-14641.53ATP binding cassette subfamily B member 1 [Source:... [more]
abcb11a5.592e-12740.14ATP binding cassette subfamily B member 11 [Source... [more]
abcb11b3.846e-12438.58bile salt export pump [Source:NCBI gene;Acc:101163... [more]
abcb81.095e-10035.66ATP binding cassette subfamily B member 8 [Source:... [more]
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BLAST of SMED30003432 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000027392.11.787e-83100.00SMESG000027392.1[more]
SMESG000027395.10.000e+067.58SMESG000027395.1[more]
SMESG000027395.10.000e+067.58SMESG000027395.1[more]
SMESG000027395.10.000e+066.85SMESG000027395.1[more]
SMESG000027399.10.000e+064.66SMESG000027399.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003432 ID=SMED30003432|Name=SMED30003432|organism=Schmidtea mediterranea sexual|type=transcript|length=1806bp
ATCAGCATTTCTTACAGTTTGAAGCCTATGAGCCACCATAACCACAGTTC
GTTTTCCTTCGGCTATTGTTTTGTCAAGGGTCTTTTGAACCAATTTCTCA
GAAATGTTATCCAGTGCCGAAGTTGCTTCGTCTAACAATAACAATTGAGG
ATTTCTTACCAATGCCATGGCAATTGCTATTCGTTGTTTCTGTCCACCAC
TGAATCCTGCCCCTCTATCTCCCAAAGGTGTATTAAATTTCTGTTTTGTT
TTCATAATAAATTCATAAGCGTTTGAATCTTGAGTAGACTTGACGATTTC
CTCATTAATGGCGTCCATTTTTCCTAACTGAATATTTTCTTGAATAGATC
CCGAGAATAGTGGAGGCTCCTGTTGAACAACTCCAATGTGAGATCGGTAC
CAGGTCAAATCGATGTCTTTTAGGTCTGTTCCACTAAAAGATATCTCCCC
AGCGAGCGGATCGTAAAACCTTTGCAGTAAATGAATGATTGTGCTCTTGC
CTGATCCACTTGGTCCAATAAAGGCCACGGTTTTTCCATAAGGCAATGTA
AAATTGAAGTTTTGAAGAATCGGTACTCCAGGCCGGGTTGGATATTCAAA
ATCTACGTTTTTAAACTCTATATCGCCGTAAAAACTTTCTGGAATGACAC
CGTGTTTCTTGTGACTAATTTGTGGCACTCTATCAATAACTTGAAACAAA
GGATAAGCGGCAGCTTTGGCAGTTATGAAATTTTCCAGCATTGGTACTGC
ATTTCCTAGCGCAATGCTTCCAATGACAATAGTCAAGAATATTTGAATAA
CAGTTCCTGGGTCATATGAGCCTTCATAAATAAACAACAATCCCATGTAG
TAAACGAGAGAGCCCACTGTGAAAATAGTGAATCCAATTACTCCCGATCC
AAGTCCTTGACAAATCGATCTTTTGACTCCCAAAGTTTCTGCTTCTTTTA
AATTGTGTGAATAACGTTTCACTTCCTTTTCTTGTGCACTGAATGCAACA
ACGGTTCGAATACTGCTGAGAATTTCTTCGGCAATTCCTCCGGCAATCGA
ATAAGCTTTGATTTGTTTGATTGAATATTTTTTAATGAAATGAATCATCA
ATGAAAAGGTTGTGGCCATTATTGGAATGGACATGCTACCGATGAAACTG
AGCTTCCATCCGGTTGTGAATGCGAGAATAATGCATCCGACAAAGGATGA
AATCTGTTGGATAAATGCGGCTAATTTGTCAGAAATGCCCGATTCAATTG
ATTCAACATCGTCGGTAAGTTTTGTCACAAGTTGGCCGGTTTGTTGCTTG
TCGAACCAGCAGATTTCTTGTCGTAAAAGCGCATTGAAATAATTCGATCT
CAATCTTCGGCTTTGGCGAGCTGCAGTGAGAGAAATCGTAACCATTTGAA
TGAATCCAGAAATAAATGTGCATGATGACAAGATTGCGAACTTGATGACC
GACGGTCTCATTCGTTCTGAGAAAGTCATTGTCAGGTCTCGGGGTGACAC
AAGCAAGTTCACGACGTCTCGGAATATTAAAACATTCAATGGAAATGCGG
CTCCAACGCAAATGCTGCTGATAATCATGACAATGACAATAAGCAGGTCG
ATTCCAGTAGCGAATCGGAACAATTGATAATATGATACGGATTCCATTGC
TGATTTCTTTGGTTTCTCTTTTTTTGCTTCTGGAATGTCAGTTTTAGATT
TTGCAGGTTTCTCTTTCAATATCGAATTCGCAAAATTTGCTTCTTCAACA
TTGTAAGAAGTTTTACCCATGTTGAAAATTGAAACTGATTTGAATAGAAT
TAAAAA
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protein sequence of SMED30003432-orf-1

>SMED30003432-orf-1 ID=SMED30003432-orf-1|Name=SMED30003432-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=602bp
FLILFKSVSIFNMGKTSYNVEEANFANSILKEKPAKSKTDIPEAKKEKPK
KSAMESVSYYQLFRFATGIDLLIVIVMIISSICVGAAFPLNVLIFRDVVN
LLVSPRDLTMTFSERMRPSVIKFAILSSCTFISGFIQMVTISLTAARQSR
RLRSNYFNALLRQEICWFDKQQTGQLVTKLTDDVESIESGISDKLAAFIQ
QISSFVGCIILAFTTGWKLSFIGSMSIPIMATTFSLMIHFIKKYSIKQIK
AYSIAGGIAEEILSSIRTVVAFSAQEKEVKRYSHNLKEAETLGVKRSICQ
GLGSGVIGFTIFTVGSLVYYMGLLFIYEGSYDPGTVIQIFLTIVIGSIAL
GNAVPMLENFITAKAAAYPLFQVIDRVPQISHKKHGVIPESFYGDIEFKN
VDFEYPTRPGVPILQNFNFTLPYGKTVAFIGPSGSGKSTIIHLLQRFYDP
LAGEISFSGTDLKDIDLTWYRSHIGVVQQEPPLFSGSIQENIQLGKMDAI
NEEIVKSTQDSNAYEFIMKTKQKFNTPLGDRGAGFSGGQKQRIAIAMALV
RNPQLLLLDEATSALDNISEKLVQKTLDKTIAEGKRTVVMVAHRLQTVRN
AD
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000231vitelline gland
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR036640ABC1_TM_sf
IPR003593AAA+_ATPase
IPR003439ABC_transporter-like
IPR011527ABC1_TM_dom
IPR027417P-loop_NTPase
IPR017871ABC_transporter_CS
Vocabulary: molecular function
TermDefinition
GO:0005524ATP binding
GO:0016887ATPase activity
GO:0042626ATPase activity, coupled to transmembrane movement of substances
Vocabulary: biological process
TermDefinition
GO:0006810transport
GO:0055085transmembrane transport
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 272..292
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 418..602
e-value: 1.3E-61
score: 210.1
NoneNo IPR availablePANTHERPTHR24222FAMILY NOT NAMEDcoord: 38..602
NoneNo IPR availableCDDcd18577ABC_6TM_Pgp_ABCB1_D1_likecoord: 83..370
e-value: 3.04425E-97
score: 297.078
NoneNo IPR availableTMHMMTMhelixcoord: 73..95
NoneNo IPR availableTMHMMTMhelixcoord: 219..241
NoneNo IPR availableTMHMMTMhelixcoord: 334..356
NoneNo IPR availableTMHMMTMhelixcoord: 119..141
NoneNo IPR availableTMHMMTMhelixcoord: 302..324
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 423..602
e-value: 6.6E-15
score: 65.5
IPR036640ABC transporter type 1, transmembrane domain superfamilyGENE3DG3DSA:1.20.1560.10coord: 53..408
e-value: 8.6E-105
score: 353.3
IPR036640ABC transporter type 1, transmembrane domain superfamilySUPERFAMILYSSF90123ABC transporter transmembrane regioncoord: 62..378
IPR011527ABC transporter type 1, transmembrane domainPFAMPF00664ABC_membranecoord: 74..347
e-value: 2.9E-55
score: 187.9
IPR011527ABC transporter type 1, transmembrane domainPROSITEPS50929ABC_TM1Fcoord: 75..362
score: 44.772
IPR003439ABC transporter-likePFAMPF00005ABC_trancoord: 414..563
e-value: 7.4E-36
score: 123.7
IPR003439ABC transporter-likePROSITEPS50893ABC_TRANSPORTER_2coord: 396..602
score: 16.68
IPR017871ABC transporter, conserved sitePROSITEPS00211ABC_TRANSPORTER_1coord: 535..549
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 386..602