SMED30003414

Overview
NameSMED30003414
Smed IDSMED30003414
Length (bp)3078
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003414 (SMED30003414) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003414 (SMED30003414) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003414 (SMED30003414) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003414 (SMED30003414) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Alignments
SMED30003414 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005523:22163..28193 -11893
Homology
BLAST of SMED30003414 vs. Planmine SMEST
Match: SMESG000022814.1 (SMESG000022814.1)

HSP 1 Score: 588.956 bits (1517), Expect = 0.000e+0
Identity = 326/459 (71.02%), Postives = 346/459 (75.38%), Query Frame = 3
Query:   96 MIPTKLDDLKLKRDVEIYVKNNSNGIKNANDINIANLKFSIKSPSLDIKDEDEIYRNLNNSKRAAQLILNLFNEFEYFKKLPISRIYGIPNCSNLDKFYGNNLTADQKPTLEDVTEALYHFVLWSAVAEYVEMKVVQLDLAEKLGITSFECKMCTNKIRIDELECSVCEESVMSYLKAKVEKGCFATETKSNKNQIGASQESNDRSDNPAGEGFIENWDCWRRGCMKKNYGNHQYCSHCGVDRAANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICEEMRYE--KDAYEIITDVEMKRASGELND-WKC--GKCECVNNYHLVSCETCGEIKEK--------------------LCNICSK-RFQAAGEICPGCNAR--------------WKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINKD 1352
            MIPTKLDDLKLKRDVEIYVKNNSNGIKNANDINIANLKFSIKSPSLDIKDEDEIYRNLNNSKRAAQLILNLFNEFEYFKKLPISRIYGIPNCSNLDKFYGNNLTADQKPTLEDVTEALYHFVLWSAVAEYVEMKVVQLDLAEKLGITSFECKMCTNKIRIDELECSVCEESVMSYLKAKVEKGCFATETKSNKNQIGASQESNDRSDNPAGEGFIENWDCWRRGCMKKNYGNHQYCSHCGVD+AANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKC ICE  R E  ++  E         A GE+++ W C    C   N  +   C  CG  K                      +CN C+   +    +IC  CN                WKC  ++CG+ N  +  YC  CG+ +      G  I     G  +++WIC KC L N D
Sbjct:    1 MIPTKLDDLKLKRDVEIYVKNNSNGIKNANDINIANLKFSIKSPSLDIKDEDEIYRNLNNSKRAAQLILNLFNEFEYFKKLPISRIYGIPNCSNLDKFYGNNLTADQKPTLEDVTEALYHFVLWSAVAEYVEMKVVQLDLAEKLGITSFECKMCTNKIRIDELECSVCEESVMSYLKAKVEKGCFATETKSNKNQIGASQESNDRSDNPAGEGFIENWDCWRRGCMKKNYGNHQYCSHCGVDKAANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCGICECSRKEERENKSEQNRGRLGNSAGGEIDENWDCWSRSCRKRNYGNHQYCSHCGVDKAANQNRGEIINVLKDGRKEEMWICNECNHFNWDINAKICKSCNESRYEIECAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINVLKDGRKEKMWICTKCGLFNGD 459          

HSP 2 Score: 479.945 bits (1234), Expect = 6.174e-156
Identity = 234/298 (78.52%), Postives = 249/298 (83.56%), Query Frame = 3
Query:  573 IDELECSVCEESVMSYLKAKVEKGCFATETKSNKNQIGASQESNDRSDNPAGEGFIENWDCWRRGCMKKNYGNHQYCSHCGVDRAANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICEEMRYEKDAYEIITDVEMKRASGELNDWKCGKCECVNNYHLVSCETCGEIKEKLCNICSKRFQAAGEICPGCNARWKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINKDYNDYCWANGYCPGRRGRLCYYCKCWVEHSQYKCPNCHR 1466
            +D  +C +CE              C   E K NK      +++  R  NPAG    ENWDCW R C K+NYGNHQYCSHCGVDRAANQNRGEIIN  KDG++EK+WICTKCG  NGDLDSDKCEICEEMRYEKDAYEIITDVEMKRA+G LNDWKCGKCECVNNY LVSC+ CGEIKEKLCNICS+RFQAAGEICPGCNARWKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINK+ N YCWANGYCPGRRGRLCYYCKCWVE+SQYKCPNC R
Sbjct:  460 LDSDKCEICE--------------CSRKEEKENK-----YEQNRGRLGNPAGGEIDENWDCWSRSCRKRNYGNHQYCSHCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGDLDSDKCEICEEMRYEKDAYEIITDVEMKRAAGVLNDWKCGKCECVNNYDLVSCKACGEIKEKLCNICSERFQAAGEICPGCNARWKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINKENNYYCWANGYCPGRRGRLCYYCKCWVEYSQYKCPNCDR 738          

HSP 3 Score: 136.732 bits (343), Expect = 1.005e-32
Identity = 87/204 (42.65%), Postives = 105/204 (51.47%), Query Frame = 3
Query:  741 ENWDCWRRGCMKKNYGNHQYCSHCGVDRAANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICEEMRYEKDAYEIITDVEMKRASGELNDWKCGKCECVNNYHLVSCETCGEIKEKLCNICSKRFQAAGEICPGCNARWKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINKD 1352
            ENW C +  C K N G+ +YCS CGVDRAANQNRGEIINVLKDGRKEKMWICTKCG  NGDLDSDKCEICE  R E+       + + ++  G L +                                    A GEI    +  W CW   C + N  N  YC +CG+ +      G  I  +  G+ ++IWIC KC L N D
Sbjct:  399 ENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEIINVLKDGRKEKMWICTKCGLFNGDLDSDKCEICECSRKEE------KENKYEQNRGRLGN-----------------------------------PAGGEI----DENWDCWSRSCRKRNYGNHQYCSHCGVDRAANQNRGEIINAFKDGKEEKIWICTKCGLFNGD 557          
BLAST of SMED30003414 vs. Planmine SMEST
Match: SMESG000022780.1 (SMESG000022780.1)

HSP 1 Score: 471.085 bits (1211), Expect = 1.600e-152
Identity = 226/243 (93.00%), Postives = 232/243 (95.47%), Query Frame = 3
Query:  741 ENWDCWRRGCMKKNYGNHQYCSHCGVDRAANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICEEMRYEKDAYEIITDVEMKRASGELNDWKCGKCECVNNYHLVSCETCGEIKEKLCNICSKRFQAAGEICPGCNARWKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINKDYNDYCWANGYCPGRRGRLCYYCKCWVEHSQYKCPNCHRY 1469
            ENW C +  C K N G+ +YCSHCGVDRAANQNR EIINVLKDG++EK+WICTKCGF NGDL SDKCEICEEMRYEKDAYEIITDVEMKRASGELNDWKCGKCECVNNYHLVSCETCGEIKEKLCNICSKRFQAAGEICPGCNARWKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINKDYNDYCWANGYCPGRRGRLCYYCKCWVEHSQYKCPNCHRY
Sbjct:  504 ENWKCLQSICGKANVGSDKYCSHCGVDRAANQNR-EIINVLKDGKEEKIWICTKCGFFNGDLGSDKCEICEEMRYEKDAYEIITDVEMKRASGELNDWKCGKCECVNNYHLVSCETCGEIKEKLCNICSKRFQAAGEICPGCNARWKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINKDYNDYCWANGYCPGRRGRLCYYCKCWVEHSQYKCPNCHRY 745          

HSP 2 Score: 284.263 bits (726), Expect = 1.331e-82
Identity = 204/488 (41.80%), Postives = 262/488 (53.69%), Query Frame = 3
Query:  135 DVEIYVKNNSNGIKNANDINIANLKFSIKSPSLDIKDEDEIYRNLNNSKRAAQLILNLFNEFEYFKKLPISRIYGIPNCSNLDKFYGNNLTADQKPTLEDVTEALYHFVLWSAVAEYVE-MKVVQLDLAEKLGITSFECKMCTNKIRIDELECSVCEESVMSYLKA----------KVEKG----CFATETKSNKNQIGASQ-------------------------ESNDRSDNPAGEGFIENWDCWRRGCMKKNYGNHQYCSHCGVDRAANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICEEMRYE--KDAYEIITDVEMKRASGELND-WKC--GKCECVNNYHLVSCETC----------GEI--------KEKL--CNICSK-RFQAAGEICPGCNAR--------------WKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWLSGECDEIWICRKCNLINKDYN 1358
            DV+ ++++N NG+K+ NDI I N      S    +K+ DEI + +NN +  +Q  LNLFNE E        +I+GI + S           A ++PT ++V E LY +VLW +V +Y+  ++  Q+D A KLGITSF+C+ C  + +IDELECS+C ESVMSYLKA          K+ K          +  NKN   +SQ                         ESNDRSDNPAG    ENWDCW RGCMK+NYGNHQYCSHCGVD+AANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICE  R E  ++  E         A GE+++ W C    C   N  +   C  C          GEI        KEK+  CN C+        +IC  CN                WKC  ++CG+ N  +  YC  CG+ +      G  I   L+G  +++WIC +CN  N+D N
Sbjct:   13 DVKTFIESNINGLKDVNDI-INNDTLKNSSKIFPLKN-DEINQMINNWQIISQSTLNLFNEPE------PKKIFGIGDSS-----------AIRRPTEKNVVEILYQYVLWHSVGKYISSVRDFQIDFAGKLGITSFKCEKCATENQIDELECSMCGESVMSYLKANNRALGELKEKINKNENNEQMNNISMKNKNDENSSQWKCEYCGNANEFLSNRCAGCPNLRRESNDRSDNPAGGEIDENWDCWWRGCMKRNYGNHQYCSHCGVDKAANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICECSRKEEKENKSEQNRGRLGNPAGGEIDENWDCWSRNCRERNYGNHQYCSHCGVDKAANQNRGEIINVFKDGRKEKMWICNGCNHFNRDINAKICKSCNESRYEIESAINKANENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEVIGVLLNGRKEKMWICNECNHFNRDIN 481          

HSP 3 Score: 92.8189 bits (229), Expect = 6.719e-19
Identity = 45/79 (56.96%), Postives = 57/79 (72.15%), Query Frame = 3
Query:  741 ENWDCWRRGCMKKNYGNHQYCSHCGVDRAANQNRGEIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICEEMRYEKDA 977
            ENW C +  C K N G+ +YCS CGVDRAANQNRGE+I VL +GRKEKMWIC +C   N D+++  C+ C E RYE ++
Sbjct:  419 ENWKCQQSICGKANVGSDKYCSRCGVDRAANQNRGEVIGVLLNGRKEKMWICNECNHFNRDINAKICKSCNESRYEIES 497          
BLAST of SMED30003414 vs. Planmine SMEST
Match: SMESG000022781.1 (SMESG000022781.1)

HSP 1 Score: 308.916 bits (790), Expect = 1.153e-98
Identity = 185/199 (92.96%), Postives = 186/199 (93.47%), Query Frame = 2
Query: 2405 MRNADSELETNENLGKKTTYEDPDLIMASLLDMEYTSRKIPNNSLFVDLTSNEESAQLLLLCTVRSITYMLSYSKTNYIDPNMSMRILKSSGVVKFMNLHGMSNTDFIGLHVGAFYVYLRSRRHLSDNQYINFVLPELAKLIFNYXXXXXXXXXXXXSYXXXXXXXXXXXXXXGQLRLETCSVKDYVLGIYTDLAYLRL 3001
            MRNADSELETNENLGKKTTYEDPDLIMASLLDMEYTSRKIPNNSLFVDLT             +RSITYMLSYSKTNYIDPNMSMRILKSSGVVKFMNLHGMSNTDFIGLHVGAFYVYLRSRRHLSDNQYINFVLPELAKLIFNYKIKEKLKIKNVKSYRDIFKIIRRLIIFRGQLRLETCSVKDYVLGIYTDLAYLRL
Sbjct:    1 MRNADSELETNENLGKKTTYEDPDLIMASLLDMEYTSRKIPNNSLFVDLT-------------IRSITYMLSYSKTNYIDPNMSMRILKSSGVVKFMNLHGMSNTDFIGLHVGAFYVYLRSRRHLSDNQYINFVLPELAKLIFNYKIKEKLKIKNVKSYRDIFKIIRRLIIFRGQLRLETCSVKDYVLGIYTDLAYLRL 186          
BLAST of SMED30003414 vs. Planmine SMEST
Match: SMESG000022934.1 (SMESG000022934.1)

HSP 1 Score: 170.629 bits (431), Expect = 1.965e-49
Identity = 92/120 (76.67%), Postives = 96/120 (80.00%), Query Frame = 3
Query:   96 MIPTKLDDLKLKRDVEIYVKNNSNGIKNANDINIANLKFSIKSPSLDIKDEDEIYRNLNNSKRAAQLILNLFNEFEYFKKLPISRIYGIPNCSNLDKFYGNNLTADQKPTLEDVTEALYH 455
            MIPTKLDD KLKRDVEI VKNNSNGIKNAND N+ANLKFSIK PSLDI DE+EIYRNLNNSKRAAQL LN+ NEFEYFKKLP SR Y IPN S            +QK TLEDVTEALYH
Sbjct:    1 MIPTKLDDSKLKRDVEINVKNNSNGIKNANDNNVANLKFSIKPPSLDINDEEEIYRNLNNSKRAAQLTLNILNEFEYFKKLPNSR-YHIPNRS----------AVEQKSTLEDVTEALYH 109          
BLAST of SMED30003414 vs. Planmine SMEST
Match: SMESG000013263.1 (SMESG000013263.1)

HSP 1 Score: 155.992 bits (393), Expect = 2.119e-42
Identity = 77/100 (77.00%), Postives = 84/100 (84.00%), Query Frame = 3
Query:  210 FSIKSPSLDIKDEDEIYRNLNNSKRAAQLILNLFNEFEYFKKLPISRIYGIPNCSNLDKFYGNNLTADQKPTLEDVTEALYHFVLWSAVAEYVEMKVVQL 509
             + K+PSL+I DE+EIYRNLNNS R  QL  NLFNEFEYFKKLPISR YGIPNCS+LDKFYGNN  A+QKP L+DVTEALYHFVL SAVAEYVEMK   L
Sbjct:   70 ITPKTPSLEINDEEEIYRNLNNSNRPDQLTKNLFNEFEYFKKLPISR-YGIPNCSSLDKFYGNNSVAEQKPILQDVTEALYHFVLLSAVAEYVEMKSGSL 168          
The following BLAST results are available for this feature:
BLAST of SMED30003414 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003414 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000022814.16.174e-15671.02SMESG000022814.1[more]
SMESG000022780.11.600e-15293.00SMESG000022780.1[more]
SMESG000022781.11.153e-9892.96SMESG000022781.1[more]
SMESG000022934.11.965e-4976.67SMESG000022934.1[more]
SMESG000013263.12.119e-4277.00SMESG000013263.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003414 ID=SMED30003414|Name=SMED30003414|organism=Schmidtea mediterranea sexual|type=transcript|length=3078bp
GTGATTTGCAAATTTTTAATTTCATCTCAAATATTGAAAAATAAATTAAA
TACTTTTAGTTTATTACGGTCAATATTAGTTTTATTTAATAGAAAATGAT
TCCCACAAAATTAGACGATTTAAAACTGAAAAGAGATGTTGAAATTTACG
TTAAAAATAATTCAAATGGCATAAAGAATGCAAATGATATTAATATTGCA
AATTTAAAATTTTCAATAAAATCGCCATCTCTTGACATAAAAGATGAAGA
CGAAATTTATCGGAATTTAAATAATTCAAAGCGAGCAGCCCAATTAATTT
TAAATCTTTTTAATGAATTTGAATATTTTAAAAAATTGCCAATTTCACGG
ATATATGGTATTCCAAATTGTTCAAATCTTGATAAATTCTATGGAAATAA
TTTAACTGCTGATCAGAAACCCACCCTGGAGGATGTTACTGAGGCTTTAT
ATCATTTCGTATTATGGTCAGCTGTTGCAGAATATGTTGAAATGAAAGTT
GTTCAACTTGATTTGGCTGAAAAACTGGGTATAACAAGTTTCGAATGCAA
AATGTGCACAAATAAGATTCGAATTGATGAATTGGAATGTTCCGTATGTG
AGGAATCCGTTATGAGCTATCTCAAGGCAAAAGTTGAAAAGGGTTGCTTT
GCAACTGAAACAAAATCAAATAAAAATCAGATTGGAGCATCACAGGAATC
GAATGATCGATCCGACAATCCCGCTGGTGAAGGATTTATTGAGAATTGGG
ATTGCTGGCGGCGGGGTTGTATGAAAAAGAATTACGGTAATCATCAATAT
TGCTCGCATTGCGGTGTTGATAGGGCAGCCAATCAGAATCGAGGAGAAAT
TATAAATGTGCTCAAGGATGGAAGGAAGGAGAAAATGTGGATTTGTACTA
AATGCGGGTTTCACAATGGAGATTTGGACTCAGATAAATGCGAGATTTGT
GAGGAAATGAGATATGAGAAAGATGCCTACGAGATAATCACAGACGTAGA
AATGAAGCGTGCCTCTGGAGAATTGAATGATTGGAAATGCGGCAAATGCG
AATGCGTGAACAATTATCATTTGGTGAGCTGCGAAACTTGTGGGGAAATC
AAGGAGAAATTATGCAATATTTGCTCCAAACGGTTTCAGGCAGCAGGTGA
AATTTGTCCCGGTTGCAATGCGAGATGGAAATGTTGGTATAATTTATGTG
GTGAAATGAATAATCGCAATGTGGATTACTGTAAATATTGCGGCCTCGGT
AAAGGTGACGCTGGGAAATACGGGATTCCAATTGCTCGCTGGCTCAGTGG
GGAATGTGATGAAATATGGATTTGTAGGAAGTGCAACCTCATCAATAAGG
ACTATAATGATTATTGCTGGGCTAACGGATATTGTCCTGGGAGAAGGGGT
CGGTTGTGCTATTATTGTAAATGCTGGGTTGAACATTCCCAATACAAATG
TCCGAACTGTCATAGATATTGATCATAATTATTCATCATTTACTGTGATA
TCTAGCTCTCATATCTGATTAATCAATAATTATGCAAATCCACACTCACT
CACACACATTAAATGCGCCTGTAACCATGGTGATATTCTCCCGTGCCTCA
TGGGGAATTATTCAGTTATTGCTCATTACCAAATCGAAATCCGTGAGAAT
TTCATGTTTCGCCATTGACATTACCATCAAAAATCAGTTTATAAAATTTC
CTATTATATCAGAATCACGTATAAAAGTCCACCGGCTGACAGCTGGGGTA
AATGCATGAATTTTGCTTTTATTGCTCGTAGAAAAGAAATTACAACAATA
AAAGTATTAACCTGCTACTGGGGATCTCCTACAATGAAATAATTCTAGGA
CTATTCATCAAAGGACTGTTCATCGAGGACTGTTCACCAGGAGTACAATT
CATCAAGAGGACTGTTCATCACAGGATGGTTCATTAACGGACGATTCATC
ATGATCTATTAATTTGCTTTGGATATATCTTACTATATGTATTATGTTAA
AATAAAGTAAATGAACAAATTAATGGAGATTGCGGAGAAAAGCCTCCGGT
GATGAAACGTCCGGATACCGTATTTCAAAAAGATAAATCCAGAAATTTTG
AATAATTTCAAAATTACAGTCAAATGCGTATTGTATATTTCTTTATTAAT
TACAGGATAAATAAAACATTTTTAGTAATTTATTTACAATTACCGGTTGT
TAATAACATTCACAGAACCTCGATATTATCCAGAGAAATTACCTGACATA
GCTTTTAATTTGGACTAAGAGTATTCAAAAGTTTCTGGATTTATCTTTTA
GAAACGTTTCATCACCGGCGGTTCAATATATAATTCTACAATATATAAAT
CAATATATTCAAAAGAAATTAATAAACAAAGCGGACGACAATTTTAATTA
GAAAATGCGTAATGCTGATTCTGAATTGGAAACTAATGAAAATCTAGGAA
AAAAGACGACCTATGAAGATCCAGATTTAATCATGGCATCTCTCCTAGAC
ATGGAATACACAAGTCGAAAGATACCAAACAATTCATTGTTTGTTGATCT
TACAAGTAATGAAGAGTCTGCTCAATTATTATTATTATGTACAGTCAGAT
CAATCACGTACATGCTCTCGTACAGTAAAACCAATTACATAGATCCAAAC
ATGTCAATGCGAATTCTCAAATCGTCAGGGGTCGTCAAGTTTATGAACCT
ACATGGCATGTCAAATACAGATTTTATTGGGTTGCATGTTGGAGCATTTT
ATGTGTATTTGCGTAGCCGAAGGCATCTTTCGGACAATCAGTACATCAAC
TTTGTTTTGCCTGAACTTGCTAAATTAATTTTTAATTATAAAATTAAAGA
GAAATTAAAAATTAAAAATGTTAAAAGTTATAGAGATATTTTCAAAATCA
TTCGAAGATTAATAATTTTCCGAGGACAATTGCGTCTTGAAACGTGTTCT
GTGAAAGATTACGTTTTGGGAATTTATACTGATTTGGCATATCTTCGTCT
GTGACATTTCAAAGATATCAAATTCAATTCTTTTGATTTCTCTGTTATAA
TTCGATCAAATAAAATAATTTTCACCCG
back to top

protein sequence of SMED30003414-orf-1

>SMED30003414-orf-1 ID=SMED30003414-orf-1|Name=SMED30003414-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
MWITVNIAASVKVTLGNTGFQLLAGSVGNVMKYGFVGSATSSIRTIMIIA
GLTDIVLGEGVGCAIIVNAGLNIPNTNVRTVIDIDHNYSSFTVISSSHI*
back to top

protein sequence of SMED30003414-orf-2

>SMED30003414-orf-2 ID=SMED30003414-orf-2|Name=SMED30003414-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=200bp
MRNADSELETNENLGKKTTYEDPDLIMASLLDMEYTSRKIPNNSLFVDLT
SNEESAQLLLLCTVRSITYMLSYSKTNYIDPNMSMRILKSSGVVKFMNLH
GMSNTDFIGLHVGAFYVYLRSRRHLSDNQYINFVLPELAKLIFNYKIKEK
LKIKNVKSYRDIFKIIRRLIIFRGQLRLETCSVKDYVLGIYTDLAYLRL*
back to top

protein sequence of SMED30003414-orf-3

>SMED30003414-orf-3 ID=SMED30003414-orf-3|Name=SMED30003414-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=459bp
MIPTKLDDLKLKRDVEIYVKNNSNGIKNANDINIANLKFSIKSPSLDIKD
EDEIYRNLNNSKRAAQLILNLFNEFEYFKKLPISRIYGIPNCSNLDKFYG
NNLTADQKPTLEDVTEALYHFVLWSAVAEYVEMKVVQLDLAEKLGITSFE
CKMCTNKIRIDELECSVCEESVMSYLKAKVEKGCFATETKSNKNQIGASQ
ESNDRSDNPAGEGFIENWDCWRRGCMKKNYGNHQYCSHCGVDRAANQNRG
EIINVLKDGRKEKMWICTKCGFHNGDLDSDKCEICEEMRYEKDAYEIITD
VEMKRASGELNDWKCGKCECVNNYHLVSCETCGEIKEKLCNICSKRFQAA
GEICPGCNARWKCWYNLCGEMNNRNVDYCKYCGLGKGDAGKYGIPIARWL
SGECDEIWICRKCNLINKDYNDYCWANGYCPGRRGRLCYYCKCWVEHSQY
KCPNCHRY*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR001876Znf_RanBP2
IPR004039Rubredoxin-type_fold
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding