GCR135

Overview
NameGCR135
Smed IDSMED30003405
Length (bp)1450
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GCR135 (SMED30003405) t-SNE clustered cells

Violin plots show distribution of expression levels for GCR135 (SMED30003405) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GCR135 (SMED30003405) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GCR135 (SMED30003405) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003405

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30003405h1SMcG0008280 dd_Smed_v4_39877_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of GCR135 vs. TrEMBL
Match: A0A193KUE1 (GCR135 OS=Schmidtea mediterranea OX=79327 GN=gcr135 PE=2 SV=1)

HSP 1 Score: 496.508 bits (1277), Expect = 8.911e-171
Identity = 230/405 (56.79%), Postives = 305/405 (75.31%), Query Frame = 3
Query:  108 MFSLRPKNYTMESINDKYRFVFSKLLKACKMSP-NNSCPTVWWYNACVGIQMVGDHTVVPCPFYKDKITLRCKSNSEWNGTERMFFDLCNGKQFLYRTQIEKYFGFVLYPMQMILLLTSIFKISIFDEMVKDFSWRMIIWSLRAKTIKYTIGWMMVLMNSNYVVSTTCIALSLIPVLADFFSLACQMALFFYVYIILFRFDVQIRTIKSALFRVTVMTPFLAIIVCLVLDLNTDDNYNYFECQTDIFGSRILHAYLTIYFAVTVILFYLWIAISRKINEMRKQVLIGTYRMEYDIVGARKEISRREIFRVRYILNWLLAHNVFDSIVLSSTIYQTI----SETPRTYYFLIMALTLFILIFGEAVTVLAIVWMNRHFRDRHNALRLWYVEINEEKNLGQEE-EDF 1304
            M+SLRPKN TM SINDKYR++FSK ++ CK++  + +CP VWWYN+C+ +    ++   PCPF K  + L+C  + +WN TE  FF LCNG +FLYR QIEKYFGF+LYP+Q+I+ L+SIFKIS++DEM+KDF WRMIIWSLRAKT+K+ IGW++VLMNSN  VS  CI +++IP+  D F  +C MALFFY+YI+L+RFDVQIRTIKSALFR+T+MTP LAI VCLVLD+N DD YNYFECQTDIFGSR++ A++T + A+T+I  YLWIAI +K  EM K+   G YRMEYDI G RKEIS+REI R  Y+L WLL  +VF++IV   ++   +     E P  + F I A+  F++   E VT++AI+W N+HF+ + NAL+ WY+EI+++K    EE E+F
Sbjct:    1 MYSLRPKNNTMASINDKYRYIFSKFIEECKLNNISYTCPNVWWYNSCIRMTNSDENIDAPCPFIKSFVALKCNPDGKWNATEIGFFQLCNGAEFLYRIQIEKYFGFILYPIQIIIYLSSIFKISLYDEMLKDFCWRMIIWSLRAKTLKHGIGWLLVLMNSNSFVSAACIPITVIPIFMDLFGQSCHMALFFYLYIMLYRFDVQIRTIKSALFRITIMTPILAITVCLVLDMNKDDYYNYFECQTDIFGSRVVLAFVTSFVAMTIINIYLWIAILKKCKEMNKEAFFGIYRMEYDIAGTRKEISKREIIRTTYVLKWLLGLSVFETIVYFCSVLHIVLDGYLERPVKFNFFITAIVTFLINNAEMVTMIAIIWFNKHFQTKQNALKQWYIEIDDDKRSNNEELENF 405          
BLAST of GCR135 vs. TrEMBL
Match: A0A193KUM0 (GCR126 OS=Schmidtea mediterranea OX=79327 GN=gcr126 PE=2 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 2.208e-61
Identity = 126/408 (30.88%), Postives = 213/408 (52.21%), Query Frame = 3
Query:  108 MFSLRPKNYTMESINDKYRFVFSKLLKACKMSPNNSCPTVWWYNACVGIQMVGDHTVVPCPFYKDKITLRCKSNSEWNGTERMFFDLCNGKQFLYRTQIEKYFGFVLYPMQMILLLTSIFKISIFDEMVKDFSWRMIIWSLRAKTIKYTIGWMMVLMNSNYVVSTTCIALSLIPVLADFFSLACQMALFFYVYIILFRFDVQIRTIKSALFRVTVMTPFLAIIVCLVLDLNTDDNYNYFECQTDIFGSRILHAYLTIYFAVTVILFYLWIAISRKINEMR-KQVLIGTYRMEYDIVGARKEISRREIFRVRYILNWLLAHNVFDSIVLSSTIYQTISETPRTYYFLIMALTLFILIFGEAVTVLAIVWMNRHFRDRHNALRLWYVEINEEKNLGQEEEDFKSALKETT 1328
            M+ L P + +ME I DK+   ++   K C    N+SC + W +  C+   M  +   + CPF+   I   C SN  W+  +R  ++ C  K+F YR  IE+ F  ++ P  +++  T ++ IS + E+VKDF+WR ++W L++++I   I   M  +NS+      C+  + +P+  D F     +ALF +VY I+F  + Q+R IKS+L     + P L  IVCL+LD+ T   ++YF+CQ     SR+ + Y+       +I F L++ I  K+  +  +  L   YRMEYDI+G RKE+S+RE+ R +  +   L  ++++ ++    +   I   P    + IM +        + + +  ++W N+  R R  A  LW   +N E    Q + DF  A++ETT
Sbjct:   29 MYYLTPVDDSMEKIEDKFSPFYNIDKKKCLKLSNSSCISTWVFQVCLTDSMRSEDNEILCPFFTTYIQPVC-SNGRWDYDDRRVYEDCYTKEFYYRLTIERTFMLLINPFILLIYQTGVYYISEYPEIVKDFNWRFVVWLLKSRSIHLFILISMCFINSSRATYEWCMLFTFLPLTLDLFYHTSMLALFIHVYAIIFNQETQLRVIKSSLKHFCFIMPLLIAIVCLILDIGT-KGHSYFQCQCGPILSRVYYVYVATTLVQILIAFPLYLRIMSKLKTIELENNLRNHYRMEYDILGERKEVSKRELVRCKLSMKVFLLLSIWNFVMYCVMVINRII-LPNRLVYKIMVIIYSSRNIADCIMLTILIWFNKGLRQRMAAWDLWNNVLNVEVKTNQNDADFLLAIQETT 433          
BLAST of GCR135 vs. TrEMBL
Match: A0A193KUM8 (GCR136 OS=Schmidtea mediterranea OX=79327 GN=gcr136 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 2.570e-48
Identity = 122/418 (29.19%), Postives = 213/418 (50.96%), Query Frame = 3
Query:  108 MFSLRPKNYTMESINDKYRFVFSKLLKACKMSPNN-SCPTVWWYNACVGI-QMVGDHTVVPCPFYKDKITLRCKSNSEWNGTERMFFDLCNGKQFLYRTQIEKYFGFVLYPMQMILLLTSIFKISIFDEMVKDFSWRMIIWSLRAKTIKYTIGWMMVLMNSNYVVSTTCIALSLIPVLADFFSLACQMALFFYVYIILFRFDVQIRTIKSALFRVTVMTPFLAIIVCLVLDLNTDDNYNYFECQTDIFGSRILHAYLTIYFAVTVILFYLWIAISRKINEMRKQVLIGT-YRMEYDIVGARKEISRREIFRVRYILNWLLAHNVFDSIVLSSTIYQTISETP--RTYYFLIMALTLFILIFGE---AVTVLAIVWMNRHFRDRHNALRLWYVEINEEKNLGQEEED-FKSALKETTDE 1334
            M+SL P + + ESI+ K+   F +    C +  ++  CP  WWY +CV +   +   + V CPFY  K  + C  N  WN + +  F  CNG  FL+R  I       L P+ +++ +TS FK+  +DE+ ++F W++IIW    K++    G ++  +NS+  +   C+ +    +  ++F++       FY+Y I+     Q+R IKSAL  +  ++P + I++ L+ D+ T      F+C+ +   S I   Y+ I  +  V + Y+ + I+  ++ +       T YR+EYDI+G RKEIS R+I R+ YIL  ++     + +V    ++   ++ P    YY+     T+ +  F +    V  + +VW+N   +    AL  WY +I+ +K L     D F +ALK  TD+
Sbjct:    1 MYSLIPDSDSAESISKKFSIFFEQEKADCLLQNDSYKCPKEWWYLSCVTLANNITTASQVQCPFYNLKTDIMC-INGTWNLSPKQLFVNCNGNLFLFRNTITNIVYKSLLPVLLLIFMTSTFKLYEYDEISQEFQWKIIIWMFIFKSVSTITGIVLASLNSSNFIYHLCMLIVTFSMYGEYFTICSMFVQCFYLYAIVNYMHTQLRIIKSALNHIMFISPIILIVIGLIFDV-TFTKGRTFQCKDEPMSSPIQFLYVYICISGIVSMIYMRVKINYSLHYLNISTNHTTVYRVEYDIIGRRKEISHRDIQRLLYILKIIICITGINILV---NVFALATKYPYVSVYYY---KFTVILYSFKDLTFVVLAVKVVWLNPTLKSLTTALERWYQDIDLKKTLDDYGNDEFFAALKVATDD 410          
BLAST of GCR135 vs. Planmine SMEST
Match: SMESG000076928.1 (SMESG000076928.1)

HSP 1 Score: 664.84 bits (1714), Expect = 0.000e+0
Identity = 327/327 (100.00%), Postives = 327/327 (100.00%), Query Frame = 3
Query:  354 MFFDLCNGKQFLYRTQIEKYFGFVLYPMQMILLLTSIFKISIFDEMVKDFSWRMIIWSLRAKTIKYTIGWMMVLMNSNYVVSTTCIALSLIPVLADFFSLACQMALFFYVYIILFRFDVQIRTIKSALFRVTVMTPFLAIIVCLVLDLNTDDNYNYFECQTDIFGSRILHAYLTIYFAVTVILFYLWIAISRKINEMRKQVLIGTYRMEYDIVGARKEISRREIFRVRYILNWLLAHNVFDSIVLSSTIYQTISETPRTYYFLIMALTLFILIFGEAVTVLAIVWMNRHFRDRHNALRLWYVEINEEKNLGQEEEDFKSALKETTDE 1334
            MFFDLCNGKQFLYRTQIEKYFGFVLYPMQMILLLTSIFKISIFDEMVKDFSWRMIIWSLRAKTIKYTIGWMMVLMNSNYVVSTTCIALSLIPVLADFFSLACQMALFFYVYIILFRFDVQIRTIKSALFRVTVMTPFLAIIVCLVLDLNTDDNYNYFECQTDIFGSRILHAYLTIYFAVTVILFYLWIAISRKINEMRKQVLIGTYRMEYDIVGARKEISRREIFRVRYILNWLLAHNVFDSIVLSSTIYQTISETPRTYYFLIMALTLFILIFGEAVTVLAIVWMNRHFRDRHNALRLWYVEINEEKNLGQEEEDFKSALKETTDE
Sbjct:    1 MFFDLCNGKQFLYRTQIEKYFGFVLYPMQMILLLTSIFKISIFDEMVKDFSWRMIIWSLRAKTIKYTIGWMMVLMNSNYVVSTTCIALSLIPVLADFFSLACQMALFFYVYIILFRFDVQIRTIKSALFRVTVMTPFLAIIVCLVLDLNTDDNYNYFECQTDIFGSRILHAYLTIYFAVTVILFYLWIAISRKINEMRKQVLIGTYRMEYDIVGARKEISRREIFRVRYILNWLLAHNVFDSIVLSSTIYQTISETPRTYYFLIMALTLFILIFGEAVTVLAIVWMNRHFRDRHNALRLWYVEINEEKNLGQEEEDFKSALKETTDE 327          
BLAST of GCR135 vs. Planmine SMEST
Match: SMESG000076929.1 (SMESG000076929.1)

HSP 1 Score: 479.559 bits (1233), Expect = 8.204e-168
Identity = 222/395 (56.20%), Postives = 296/395 (74.94%), Query Frame = 3
Query:  138 MESINDKYRFVFSKLLKACKMSP-NNSCPTVWWYNACVGIQMVGDHTVVPCPFYKDKITLRCKSNSEWNGTERMFFDLCNGKQFLYRTQIEKYFGFVLYPMQMILLLTSIFKISIFDEMVKDFSWRMIIWSLRAKTIKYTIGWMMVLMNSNYVVSTTCIALSLIPVLADFFSLACQMALFFYVYIILFRFDVQIRTIKSALFRVTVMTPFLAIIVCLVLDLNTDDNYNYFECQTDIFGSRILHAYLTIYFAVTVILFYLWIAISRKINEMRKQVLIGTYRMEYDIVGARKEISRREIFRVRYILNWLLAHNVFDSIVLSSTIYQTI----SETPRTYYFLIMALTLFILIFGEAVTVLAIVWMNRHFRDRHNALRLWYVEINEEKNLGQEE-EDF 1304
            M SINDKYR++FSK ++ CK++  + +CP VWWYN+C+ +    ++   PCPF K  + L+C  + +WN TE  FF LCNG +FLYR QIEKYFGF+LYP+Q+I+ L+SIFKIS++DEM+KDF WRMIIWSLRAKT+K+ IGW++VLMNSN  VS  CI +++IP+  D F  +C MALFFY+YI+L+RFDVQIRTIKSALFR+T+MTP LAI VCLVLD+N DD YNYFECQTDIFGSR++ A++T + A+T+I  YLWIAI +K  EM K+   G YRMEYDI G RKEIS+REI R  Y+L WLL  +VF++IV   ++   +     E P  + F I A+  F++   E VT++AI+W N+HF+ + NAL+ WY+EI+++K    EE E+F
Sbjct:    1 MASINDKYRYIFSKFIEECKLNNISYTCPNVWWYNSCIRMTNSDENIDAPCPFIKSFVALKCNPDGKWNATEIGFFQLCNGAEFLYRIQIEKYFGFILYPIQIIIYLSSIFKISLYDEMLKDFCWRMIIWSLRAKTLKHGIGWLLVLMNSNSFVSAACIPITVIPIFMDLFGQSCHMALFFYLYIMLYRFDVQIRTIKSALFRITIMTPILAITVCLVLDMNKDDYYNYFECQTDIFGSRVVLAFVTSFVAMTIINIYLWIAILKKCKEMNKEAFFGIYRMEYDIAGTRKEISKREIIRTTYVLKWLLGLSVFETIVYFCSVLHIVLDGYLERPVKFNFFITAIVTFLINNAEMVTMIAIIWFNKHFQTKQNALKQWYIEIDDDKRSNNEELENF 395          
BLAST of GCR135 vs. Planmine SMEST
Match: SMESG000076933.1 (SMESG000076933.1)

HSP 1 Score: 216.468 bits (550), Expect = 2.953e-65
Identity = 126/408 (30.88%), Postives = 213/408 (52.21%), Query Frame = 3
Query:  108 MFSLRPKNYTMESINDKYRFVFSKLLKACKMSPNNSCPTVWWYNACVGIQMVGDHTVVPCPFYKDKITLRCKSNSEWNGTERMFFDLCNGKQFLYRTQIEKYFGFVLYPMQMILLLTSIFKISIFDEMVKDFSWRMIIWSLRAKTIKYTIGWMMVLMNSNYVVSTTCIALSLIPVLADFFSLACQMALFFYVYIILFRFDVQIRTIKSALFRVTVMTPFLAIIVCLVLDLNTDDNYNYFECQTDIFGSRILHAYLTIYFAVTVILFYLWIAISRKINEMR-KQVLIGTYRMEYDIVGARKEISRREIFRVRYILNWLLAHNVFDSIVLSSTIYQTISETPRTYYFLIMALTLFILIFGEAVTVLAIVWMNRHFRDRHNALRLWYVEINEEKNLGQEEEDFKSALKETT 1328
            M+ L P + +ME I DK+   ++   K C    N+SC + W +  C+   M  +   + CPF+   I   C SN  W+  +R  ++ C  K+F YR  IE+ F  ++ P  +++  T ++ IS + E+VKDF+WR ++W L++++I   I   M  +NS+      C+  + +P+  D F     +ALF +VY I+F  + Q+R IKS+L     + P L  IVCL+LD+ T   ++YF+CQ     SR+ + Y+       +I F L++ I  K+  +  +  L   YRMEYDI+G RKE+S+RE+ R +  +   L  ++++ ++    +   I   P    + IM +        + + +  ++W N+  R R  A  LW   +N E    Q + DF  A++ETT
Sbjct:    1 MYYLTPVDDSMEKIEDKFSPFYNIDKKKCLKLSNSSCISTWVFQVCLTDSMRSEDNEILCPFFTTYIQPVC-SNGRWDYDDRRVYEDCYTKEFYYRLTIERTFMLLINPFILLIYQTGVYYISEYPEIVKDFNWRFVVWLLKSRSIHLFILISMCFINSSRATYEWCMLFTFLPLTLDLFYHTSMLALFIHVYAIIFNQETQLRVIKSSLKHFCFIMPLLIAIVCLILDIGT-KGHSYFQCQCGPILSRVYYVYVATTLVQILIAFPLYLRIMSKLKTIELENNLRNHYRMEYDILGERKEVSKRELVRCKLSMKVFLLLSIWNFVMYCVMVINRII-LPNRLVYKIMVIIYSSRNIADCIMLTILIWFNKGLRQRMAAWDLWNNVLNVEVKTNQNDADFLLAIQETT 405          
BLAST of GCR135 vs. Planmine SMEST
Match: SMESG000018582.1 (SMESG000018582.1)

HSP 1 Score: 162.155 bits (409), Expect = 2.293e-45
Identity = 98/316 (31.01%), Postives = 168/316 (53.16%), Query Frame = 3
Query:  108 MFSLRPKNYTMESINDKYRFVFSKLLKACKMSPNN-SCPTVWWYNACVGI-QMVGDHTVVPCPFYKDKITLRCKSNSEWNGTERMFFDLCNGKQFLYRTQIEKYFGFVLYPMQMILLLTSIFKISIFDEMVKDFSWRMIIWSLRAKTIKYTIGWMMVLMNSNYVVSTTCIALSLIPVLADFFSLACQMALFFYVYIILFRFDVQIRTIKSALFRVTVMTPFLAIIVCLVLDLNTDDNYNYFECQTDIFGSRILHAYLTIYFAVTVILFYLWIAISRKINEMRKQVLIGT-YRMEYDIVGARKEISRREIFRVRYIL 1046
            M+SL P + + ESI+ K+   F +    C +  ++  CP  WWY +CV +   +   + V CPFY  K  + C  N  WN + +  F  CNG  FL+R  I       L P+ +++ +TS FK+  +DE+ ++F W++IIW    K++    G ++  +NS+  +   C+ +    +  ++F++       FY+Y I+     Q+R IKSAL  +  ++P + I++ L+ D+ T      F+C+ +   S I   Y+ I  +  V + Y+ + I+  ++ +       T YR+EYDI+G RKEIS R+I R+ YIL
Sbjct:    1 MYSLIPDSDSAESISKKFSIFFEQEKADCLLQNDSYKCPKEWWYLSCVTLANNITAASQVQCPFYNLKTDIMCI-NGTWNLSPKQLFVNCNGNLFLFRNTITNIVYKSLLPVLLLIFMTSTFKLYEYDEISQEFQWKIIIWMFIFKSVSTITGIVLASLNSSNFIYHLCMLIVTFSMYGEYFTICSMFVQCFYLYAIVNYMHTQLRIIKSALNHIMFISPIILIVIGLIFDV-TFTKGRTFQCKDEPMSSPIQFLYVYICISGIVSMIYMRVKINYSLHYLNISTNHTTVYRVEYDIIGRRKEISHRDIQRLLYIL 314          
The following BLAST results are available for this feature:
BLAST of GCR135 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
A0A193KUE18.911e-17156.79GCR135 OS=Schmidtea mediterranea OX=79327 GN=gcr13... [more]
A0A193KUM02.208e-6130.88GCR126 OS=Schmidtea mediterranea OX=79327 GN=gcr12... [more]
A0A193KUM82.570e-4829.19GCR136 OS=Schmidtea mediterranea OX=79327 GN=gcr13... [more]
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BLAST of GCR135 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of GCR135 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000076928.10.000e+0100.00SMESG000076928.1[more]
SMESG000076929.18.204e-16856.20SMESG000076929.1[more]
SMESG000076933.12.953e-6530.88SMESG000076933.1[more]
SMESG000018582.12.293e-4531.01SMESG000018582.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003405 ID=SMED30003405|Name=GCR135|organism=Schmidtea mediterranea sexual|type=transcript|length=1450bp
TTTTGACTAGAAATTTGAAGACATTGATATAAAACCTAACCTTAAGTTAT
TTATCCTTATCATTACTTTGTTAATTGATTATAATTAATAATTAATTAAA
TTTCAAAATGTTTTCTTTACGACCAAAAAACTACACAATGGAGTCAATCA
ACGACAAATACCGATTCGTGTTTTCGAAGTTATTAAAAGCTTGTAAAATG
TCTCCGAACAACTCTTGTCCAACCGTCTGGTGGTACAATGCCTGTGTAGG
AATCCAAATGGTTGGTGATCACACTGTTGTCCCCTGTCCGTTTTATAAAG
ACAAAATCACTCTCAGGTGCAAATCGAACAGCGAATGGAATGGCACTGAA
CGCATGTTTTTCGATCTTTGTAATGGAAAACAATTTCTCTATCGAACACA
GATTGAAAAATACTTCGGATTCGTTCTTTATCCTATGCAAATGATTTTGC
TATTGACTAGCATTTTTAAGATTTCCATTTTCGATGAAATGGTGAAAGAT
TTTTCTTGGAGAATGATCATTTGGTCTTTAAGAGCGAAGACGATTAAGTA
CACAATTGGCTGGATGATGGTGCTGATGAATTCCAACTACGTCGTTTCGA
CAACTTGCATTGCTTTGAGTCTTATTCCGGTTTTGGCCGACTTTTTCAGT
TTAGCTTGTCAAATGGCGCTCTTTTTCTATGTTTATATTATTTTGTTTCG
ATTCGATGTACAAATTCGGACGATCAAGTCGGCATTATTCCGAGTTACCG
TAATGACTCCATTTCTAGCTATTATAGTTTGTCTCGTTCTGGATTTGAAT
ACAGACGACAACTATAACTATTTTGAATGCCAAACTGACATTTTCGGTTC
AAGAATTTTACATGCATATTTGACAATTTACTTTGCCGTAACAGTTATAC
TTTTTTATTTATGGATTGCTATTTCTAGAAAAATTAATGAAATGCGAAAA
CAAGTTCTAATTGGGACATATAGGATGGAATATGACATTGTAGGAGCCAG
GAAAGAGATTTCAAGGAGAGAAATATTTCGTGTGAGATATATTTTAAATT
GGTTACTAGCACATAACGTATTTGACTCGATAGTTCTTAGTTCAACTATT
TATCAAACGATTTCGGAAACTCCAAGAACATACTATTTTCTTATAATGGC
TTTAACATTATTTATATTAATATTCGGAGAAGCAGTTACTGTTCTTGCTA
TAGTCTGGATGAACAGGCATTTCCGAGACCGACACAATGCTCTTCGATTG
TGGTATGTTGAAATTAATGAAGAGAAAAATCTAGGACAAGAAGAAGAAGA
CTTCAAGTCGGCACTAAAGGAAACGACAGATGAAAATATTTTCATAAATT
AACATTTTATTTAGAATTTTTCAAAATTATTTTCCAGTGTAAATACTAGA
TAAACCAACACAAATTGTTTATCGAATGTAATGAATATAATTCACAGCAG
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protein sequence of SMED30003405-orf-1

>SMED30003405-orf-1 ID=SMED30003405-orf-1|Name=SMED30003405-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=415bp
MFSLRPKNYTMESINDKYRFVFSKLLKACKMSPNNSCPTVWWYNACVGIQ
MVGDHTVVPCPFYKDKITLRCKSNSEWNGTERMFFDLCNGKQFLYRTQIE
KYFGFVLYPMQMILLLTSIFKISIFDEMVKDFSWRMIIWSLRAKTIKYTI
GWMMVLMNSNYVVSTTCIALSLIPVLADFFSLACQMALFFYVYIILFRFD
VQIRTIKSALFRVTVMTPFLAIIVCLVLDLNTDDNYNYFECQTDIFGSRI
LHAYLTIYFAVTVILFYLWIAISRKINEMRKQVLIGTYRMEYDIVGARKE
ISRREIFRVRYILNWLLAHNVFDSIVLSSTIYQTISETPRTYYFLIMALT
LFILIFGEAVTVLAIVWMNRHFRDRHNALRLWYVEINEEKNLGQEEEDFK
SALKETTDENIFIN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableTMHMMTMhelixcoord: 250..272
NoneNo IPR availableTMHMMTMhelixcoord: 208..230
NoneNo IPR availableTMHMMTMhelixcoord: 345..367
NoneNo IPR availableTMHMMTMhelixcoord: 146..168
NoneNo IPR availableTMHMMTMhelixcoord: 313..335
NoneNo IPR availableTMHMMTMhelixcoord: 173..195
NoneNo IPR availableTMHMMTMhelixcoord: 103..125