Eukaryotic translation initiation factor 2-alpha kinase 1

Overview
NameEukaryotic translation initiation factor 2-alpha kinase 1
Smed IDSMED30003352
Length (bp)1829
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Eukaryotic translation initiation factor 2-alpha kinase 1 (SMED30003352) t-SNE clustered cells

Violin plots show distribution of expression levels for Eukaryotic translation initiation factor 2-alpha kinase 1 (SMED30003352) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Eukaryotic translation initiation factor 2-alpha kinase 1 (SMED30003352) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Eukaryotic translation initiation factor 2-alpha kinase 1 (SMED30003352) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003352

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30003352h1SMcG0007762 SmedASXL_011593SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Human
Match: EIF2AK1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:HGNC Symbol;Acc:HGNC:24921])

HSP 1 Score: 165.236 bits (417), Expect = 4.979e-43
Identity = 139/478 (29.08%), Postives = 218/478 (45.61%), Query Frame = 2
Query:  335 ISFVQNFK--PIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFE-------RFEEAN--------WNSQVENFTNCS----GSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNV-----PKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRC----------LARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEI----------SSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKES 1630
            IS +Q  +   +  + + ++ +N+F+ +  +GKGG+GRVY   NK+D Q YAIK+++I  +   +      ++E ++LA L H NIV YHT W E       R + A          + Q E+   C      S ++SI  I    +         S + + N  +V   TN     +G L       S ++ Q +      ++ I E             SH  E   + E     NVN       +    L+IQM+ C L+L DW+  RNK      D    E  C          + ++L++GV YIH+  ++HRD+KP NIFL  H   D Q+KIGDFGL+       C +I          +  R    T+  GT  Y++PE   G      YD KSD+YS+G++LLEL   F T+ E   V+  +  G   + L +  P++ +++++L       RP A  +L Q+  F+ S
Sbjct:  143 ISRIQKIRSREVALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGA--TKTVCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRADRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSI--IFAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELE------STLELQENGLAGLSASSIVEQQLPLRR-----NSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVD----ESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFL--H-GPDQQVKIGDFGLA-------CTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGS----EYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLL-QSELFQNS 586          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Human
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:HGNC Symbol;Acc:HGNC:19687])

HSP 1 Score: 124.02 bits (310), Expect = 1.281e-28
Identity = 131/477 (27.46%), Postives = 207/477 (43.40%), Query Frame = 2
Query:  296 GDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEA--------NWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGS---------LSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVP-KMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKE-VRC--LARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSK---FFEFDK------CDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMV-KFSTQSELCHVVEDISKGIGFKDLIETWPLED----QHVRN------LISNKPENRPEASFIL 1603
            G +++      N +     F   + QF  ++   +F+ ++ +GKG FG V    NK+D   YA+K++ IN +    + I     E  +L++L+H NIVRY+  W ER E          +     ++     G  A+    +    +      +  S   S +G   +S     A   GS             ++ +S +   +S+     S+IIF+     N   +S + +  ED      C  S  +V  +    LYIQME+C          ++ L  T+    +++ VR   L R++L G+ YIH   +IHRD+KP NIFL      D  +KIGDFGL+     F  D        D I S     LT   GTA Y +PEV  G  K  +Y+ K DL+S+G+I  E+      T SE   V+  +      K     +P ED    +H +       L+++ P  RP A+ +L
Sbjct:  557 GQDYVETVIPSNRLPSAAFFSETQRQF--SRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKG---EVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSAS-ARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSE-----SDIIFD-----NEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYC---------EKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFL----DSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEV-QGSTKS-AYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPK-----FP-EDFDDGEHAKQKSVISWLLNHDPAKRPTATELL 996          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Human
Match: EIF2AK3 (eukaryotic translation initiation factor 2 alpha kinase 3 [Source:HGNC Symbol;Acc:HGNC:3255])

HSP 1 Score: 115.161 bits (287), Expect = 5.560e-26
Identity = 70/199 (35.18%), Postives = 112/199 (56.28%), Query Frame = 2
Query: 1013 LYIQMEFCHL-NLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKM----KLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDL-IETWPLEDQHVRNLISNKPENRPEA 1591
            LYIQM+ C   NL+DW+  R     T+++ +      +  Q+ + VE++HS  ++HRD+KP+NIF  +    D  +K+GDFGL    + D+ +E + +  M    + T   GT  Y +PE  +G     SY HK D++S+G+IL EL+  FSTQ E    + D+ + + F  L  + +P E   V++++S  P  RPEA
Sbjct:  884 LYIQMQLCRKENLKDWMNGR----CTIEERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTM----DDVVKVGDFGLVTAMDQDE-EEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGN----SYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDV-RNLKFPPLFTQKYPCEYVMVQDMLSPSPMERPEA 1068          

HSP 2 Score: 66.2402 bits (160), Expect = 1.288e-10
Identity = 34/96 (35.42%), Postives = 57/96 (59.38%), Query Frame = 2
Query:  362 IKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVE 649
            IK    +++ + DF+ I+ +G+GGFG V+ A NK+D   YAIK++ +   +L  +++   ++E + LAKL H  IVRY   W E   E  W  +++
Sbjct:  580 IKNSGYISRYLTDFEPIQCLGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRELAREKV---MREVKALAKLEHPGIVRYFNAWLEAPPE-KWQEKMD 671          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Human
Match: EIF2AK2 (eukaryotic translation initiation factor 2 alpha kinase 2 [Source:HGNC Symbol;Acc:HGNC:9437])

HSP 1 Score: 103.99 bits (258), Expect = 7.006e-23
Identity = 77/213 (36.15%), Postives = 108/213 (50.70%), Query Frame = 2
Query: 1013 LYIQMEFCHLN-LQDWLCNRN--KLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQN 1642
            L+IQMEFC    L+ W+  R   KL   L    F+       Q+ KGV+YIHS  +IHRD+KP+NIFL+       Q+KIGDFGL    + D           K T + GT  Y +PE  + +     Y  + DLY++G+IL EL+    T  E      D+  GI   D+ +    E   ++ L+S KPE+RP  S IL     +K+S  +N
Sbjct:  321 LFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFE-------QITKGVDYIHSKKLIHRDLKPSNIFLV----DTKQVKIGDFGLVTSLKNDG----------KRTRSKGTLRYMSPEQISSQ----DYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGI-ISDIFDKK--EKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKN 505          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Human
Match: EIF2AK2 (eukaryotic translation initiation factor 2 alpha kinase 2 [Source:HGNC Symbol;Acc:HGNC:9437])

HSP 1 Score: 103.99 bits (258), Expect = 8.454e-23
Identity = 77/214 (35.98%), Postives = 111/214 (51.87%), Query Frame = 2
Query: 1013 LYIQMEFCHLN-LQDWLCNRN--KLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIR-KMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQN 1642
            L+IQMEFC    L+ W+  R   KL   L    F+       Q+ KGV+YIHS  +IHRD+KP+NIFL+       Q+KIGDFGL           ++S++   K T + GT  Y +PE  + +     Y  + DLY++G+IL EL+    T  E      D+  GI   D+ +    E   ++ L+S KPE+RP  S IL     +K+S  +N
Sbjct:  362 LFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFE-------QITKGVDYIHSKKLIHRDLKPSNIFLV----DTKQVKIGDFGL-----------VTSLKNDGKRTRSKGTLRYMSPEQISSQ----DYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGI-ISDIFDKK--EKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKN 546          

HSP 2 Score: 58.5362 bits (140), Expect = 2.691e-8
Identity = 32/70 (45.71%), Postives = 43/70 (61.43%), Query Frame = 2
Query:  398 DFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVW 607
            DF  I+ IG GGFG+V+ A ++ID + Y IK+V  N    NEK      +E + LAKL+H NIV Y+  W
Sbjct:  266 DFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYN----NEK----AEREVKALAKLDHVNIVHYNGCW 327          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Celegans
Match: pek-1 (Eukaryotic translation initiation factor 2-alpha kinase pek-1 [Source:UniProtKB/Swiss-Prot;Acc:Q19192])

HSP 1 Score: 101.679 bits (252), Expect = 4.485e-22
Identity = 67/199 (33.67%), Postives = 102/199 (51.26%), Query Frame = 2
Query: 1013 LYIQMEFC-HLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQ-LKIGDFGLSKFFEFDKCDEIS----SIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEA 1591
            LYI M+ C    L+DW+  R+K   T++      ++   +QL  G+EY+H+   IHRD+KP N+F  +  +   Q LKIGD GL+   + D   +I+    S    K T N GT  Y +PE    + K   Y  K D++++G++  EL++ FST SE  H   D  KG     +++  P     +  L S +P  RP A
Sbjct:  880 LYIVMQLCAEKTLEDWI-RRSK---TMESRPLFTMKNWIKQLASGLEYLHNKGFIHRDLKPGNVFFSLDSTHGHQILKIGDLGLAT--KTDGAPKITVRQDSDSSAKHTKNVGTRSYMSPE----QLKHQQYTEKVDIFALGLVATELIISFSTASERIHTFADFQKG-DIPAILDNVPESRDFLLQLTSLEPSERPTA 1067          

HSP 2 Score: 54.299 bits (129), Expect = 2.696e-7
Identity = 30/92 (32.61%), Postives = 47/92 (51.09%), Query Frame = 2
Query:  380 VNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENF 655
             ++  N+F+  K IG GGFG V+ A +  D   YA+K++ +     N+K     ++E R LA  +H  I+RY   W ER  +     + EN 
Sbjct:  597 TSRFANEFEVKKVIGHGGFGVVFRAQSITDMNEYAVKRIAVAD---NDKARNRVLREARALAMFDHPGIIRYFYAWEERPPKGFQEKEDENL 685          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Celegans
Match: Y38E10A.8 (pep chromosome:WBcel235:II:12607200:12608788:1 gene:WBGene00012586.1 transcript:Y38E10A.8.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y38E10A.8)

HSP 1 Score: 77.411 bits (189), Expect = 2.170e-15
Identity = 61/205 (29.76%), Postives = 96/205 (46.83%), Query Frame = 2
Query: 1013 LYIQMEFCHLN-LQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKL-----------TANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEA 1591
            +YI ME C  N L+DW+    K   T +   +  ++   +QL   ++++HSNN IHRD+KP N+F     S+  +LKIGDFGL+          I  ++  K            TA  GT  Y APE       R  Y+ K D++++G+I  EL++  +   E+     DI +   +    + +P   + +  L    P  RP A
Sbjct:   94 MYICMELCEQNTLKDWI----KGNRTARSRPWMLMKDWIKQLACALDHLHSNNFIHRDLKPENVFF-ARDSQFKKLKIGDFGLT-------TRAIEGLKDWKQRTQSQEAGQNHTAGAGTPSYMAPEQI-----RNQYNEKVDIFALGLIAAELIIINTPSDEMG--TNDIIRSGQWPIAWQDYPDAIEFLSQLTHMNPTMRPSA 279          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Celegans
Match: wee-1.3 (Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase wee-1.3 [Source:UniProtKB/Swiss-Prot;Acc:O18209])

HSP 1 Score: 76.6406 bits (187), Expect = 2.449e-14
Identity = 51/150 (34.00%), Postives = 75/150 (50.00%), Query Frame = 2
Query: 1013 LYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQS 1462
            LYIQ E C  +L  +   ++ L          E+  +   LL+ V ++HSN++IH DIKP NIFL    +KD+  K+GDFGL         +++ S       A  G + Y APEV NG+  +      SD++S+GM +LE        S
Sbjct:  179 LYIQTELCDQSLLKYCTEKHALPE-------DEIWNIFVDLLQAVHHLHSNDMIHDDIKPENIFL----TKDMICKLGDFGL--VINLKNPNDVKS-------AEEGDSKYLAPEVLNGRPTK-----SSDIFSLGMTILEATTDLDVPS 303          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Celegans
Match: gck-1 (Germinal center kinase 1 [Source:UniProtKB/Swiss-Prot;Acc:H2L099])

HSP 1 Score: 76.2554 bits (186), Expect = 3.129e-14
Identity = 60/210 (28.57%), Postives = 97/210 (46.19%), Query Frame = 2
Query: 1013 LYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSEL-------------CHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFIL 1603
            L+I ME+        L    KL       D   +  + R++LKG+EY+HS   IHRDIK AN+ +  H      +K+ DFG+          +++   K ++T   G+ F+ APE+     K+ SYD+K+D++S+G+  +EL       S+L               V++       FK+ +E            ++  PENRP AS +L
Sbjct:   90 LWIIMEYLGGGSALDLTKSGKL-------DESHIAVILREILKGLEYLHSERKIHRDIKAANVLVSEH----GDVKVADFGV--------AGQLTETVKKRITF-VGSPFWMAPELI----KQSSYDYKADIWSLGITAIELANGEPPHSDLHPMRVLFLIPKNPPPVLQGSQWSKPFKEFVEM----------CLNKDPENRPSASTLL 265          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Celegans
Match: gck-1 (Germinal center kinase 1 [Source:UniProtKB/Swiss-Prot;Acc:H2L099])

HSP 1 Score: 76.2554 bits (186), Expect = 3.138e-14
Identity = 60/210 (28.57%), Postives = 97/210 (46.19%), Query Frame = 2
Query: 1013 LYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSEL-------------CHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFIL 1603
            L+I ME+        L    KL       D   +  + R++LKG+EY+HS   IHRDIK AN+ +  H      +K+ DFG+          +++   K ++T   G+ F+ APE+     K+ SYD+K+D++S+G+  +EL       S+L               V++       FK+ +E            ++  PENRP AS +L
Sbjct:  105 LWIIMEYLGGGSALDLTKSGKL-------DESHIAVILREILKGLEYLHSERKIHRDIKAANVLVSEH----GDVKVADFGV--------AGQLTETVKKRITF-VGSPFWMAPELI----KQSSYDYKADIWSLGITAIELANGEPPHSDLHPMRVLFLIPKNPPPVLQGSQWSKPFKEFVEM----------CLNKDPENRPSASTLL 280          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Fly
Match: PEK (gene:FBgn0037327 transcript:FBtr0330027)

HSP 1 Score: 111.309 bits (277), Expect = 5.530e-25
Identity = 69/206 (33.50%), Postives = 105/206 (50.97%), Query Frame = 2
Query: 1013 LYIQMEFCHL-NLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFE-----FDKCDEISSIRK-MKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQ 1609
            LYIQM+ C   +L+DWL +           D      +  Q++  V+Y+H   +IHRD+KP+NIF     S+D Q+KIGDFGL            KC + S +    + T   GT  Y +PE   G+     YD+K D+YS+G+I  EL V FST+ E    +  +  G   KD    +P +   ++ ++S +PE RP+   + +Q
Sbjct:  866 LYIQMQLCRKESLRDWLRDNRSETRAAHIGD------IFHQIVDAVDYVHLKGLIHRDLKPSNIFF----SQDGQIKIGDFGLVTDMADIPNLVAKCGDQSGLPSCARHTQQVGTHLYMSPEQLLGQ----HYDYKVDIYSLGLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLLQQMLSAQPEQRPQTKQLKSQ 1057          

HSP 2 Score: 60.077 bits (144), Expect = 5.688e-9
Identity = 39/141 (27.66%), Postives = 72/141 (51.06%), Query Frame = 2
Query:  191 FYVIDNNVIISNTSDNAEEKTKLLVISVDIFKKCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFE 613
            + VI+ +V +    +  E  T+ L+  V   ++  G+ F  P    N  +  ++       +  ++  +DF+ ++ +G+GGFG V+ A NK+D   YAIK++ +   + + + +   ++E R LA   H NIVRY   W E
Sbjct:  511 YLVIERHVPVQTAIEATEASTQALLGPVVPMQRP-GNRFSFPPGQANQRTISESTTHSGEHY-TSRFQSDFELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRV---LREARTLASCEHHNIVRYFHSWTE 646          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Fly
Match: PEK (gene:FBgn0037327 transcript:FBtr0078770)

HSP 1 Score: 110.923 bits (276), Expect = 7.247e-25
Identity = 69/206 (33.50%), Postives = 105/206 (50.97%), Query Frame = 2
Query: 1013 LYIQMEFCHL-NLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFE-----FDKCDEISSIRK-MKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQ 1609
            LYIQM+ C   +L+DWL +           D      +  Q++  V+Y+H   +IHRD+KP+NIF     S+D Q+KIGDFGL            KC + S +    + T   GT  Y +PE   G+     YD+K D+YS+G+I  EL V FST+ E    +  +  G   KD    +P +   ++ ++S +PE RP+   + +Q
Sbjct:  929 LYIQMQLCRKESLRDWLRDNRSETRAAHIGD------IFHQIVDAVDYVHLKGLIHRDLKPSNIFF----SQDGQIKIGDFGLVTDMADIPNLVAKCGDQSGLPSCARHTQQVGTHLYMSPEQLLGQ----HYDYKVDIYSLGLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLLQQMLSAQPEQRPQTKQLKSQ 1120          

HSP 2 Score: 60.077 bits (144), Expect = 5.342e-9
Identity = 39/141 (27.66%), Postives = 72/141 (51.06%), Query Frame = 2
Query:  191 FYVIDNNVIISNTSDNAEEKTKLLVISVDIFKKCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFE 613
            + VI+ +V +    +  E  T+ L+  V   ++  G+ F  P    N  +  ++       +  ++  +DF+ ++ +G+GGFG V+ A NK+D   YAIK++ +   + + + +   ++E R LA   H NIVRY   W E
Sbjct:  574 YLVIERHVPVQTAIEATEASTQALLGPVVPMQRP-GNRFSFPPGQANQRTISESTTHSGEHY-TSRFQSDFELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRV---LREARTLASCEHHNIVRYFHSWTE 709          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Fly
Match: PEK (gene:FBgn0037327 transcript:FBtr0330026)

HSP 1 Score: 110.923 bits (276), Expect = 7.374e-25
Identity = 69/206 (33.50%), Postives = 105/206 (50.97%), Query Frame = 2
Query: 1013 LYIQMEFCHL-NLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFE-----FDKCDEISSIRK-MKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQ 1609
            LYIQM+ C   +L+DWL +           D      +  Q++  V+Y+H   +IHRD+KP+NIF     S+D Q+KIGDFGL            KC + S +    + T   GT  Y +PE   G+     YD+K D+YS+G+I  EL V FST+ E    +  +  G   KD    +P +   ++ ++S +PE RP+   + +Q
Sbjct:  877 LYIQMQLCRKESLRDWLRDNRSETRAAHIGD------IFHQIVDAVDYVHLKGLIHRDLKPSNIFF----SQDGQIKIGDFGLVTDMADIPNLVAKCGDQSGLPSCARHTQQVGTHLYMSPEQLLGQ----HYDYKVDIYSLGLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLLQQMLSAQPEQRPQTKQLKSQ 1068          

HSP 2 Score: 60.077 bits (144), Expect = 5.558e-9
Identity = 39/141 (27.66%), Postives = 72/141 (51.06%), Query Frame = 2
Query:  191 FYVIDNNVIISNTSDNAEEKTKLLVISVDIFKKCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFE 613
            + VI+ +V +    +  E  T+ L+  V   ++  G+ F  P    N  +  ++       +  ++  +DF+ ++ +G+GGFG V+ A NK+D   YAIK++ +   + + + +   ++E R LA   H NIVRY   W E
Sbjct:  522 YLVIERHVPVQTAIEATEASTQALLGPVVPMQRP-GNRFSFPPGQANQRTISESTTHSGEHY-TSRFQSDFELMQCLGRGGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRV---LREARTLASCEHHNIVRYFHSWTE 657          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Fly
Match: Gcn2 (gene:FBgn0019990 transcript:FBtr0334552)

HSP 1 Score: 83.5741 bits (205), Expect = 3.038e-16
Identity = 75/262 (28.63%), Postives = 118/262 (45.04%), Query Frame = 2
Query:  980 SNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS--KFFEFDKCD-------EISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLEL-MVKFSTQSELCHVVEDISK-GIGFKDLIETWPLEDQHVRN---LISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTL 1723
            +N   P M+  +YIQMEFC        C     +     +D   +  L R++ +G+ +IH   +IHRD+KP NIFL  H     Q+KIGDFGL+   F      D       +I+S      T   GT  Y APE   G   +  Y+ K D+Y++G+IL E+    F T  E    +  +    I   D +   P  ++ V+    L+++ P  RP A  +L         +  N L   ++  ++ N + K+   L+ + L
Sbjct:  686 TNSPKPLMQ-VMYIQMEFCEK------CTLRTAIDDNLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHD----QIKIGDFGLATTSFLALQAHDAAPAPVNQITSAEDGTGTGKVGTTLYVAPE-LTGNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKTVKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALA-NPQSKAYKNLVARCL 934          

HSP 2 Score: 55.8398 bits (133), Expect = 1.143e-7
Identity = 31/106 (29.25%), Postives = 55/106 (51.89%), Query Frame = 2
Query:  314 PNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEAN 631
            PN +Q + S +    P       ++L  +F+ +  +GKG FG V    N +D + YAIK++ +     + +      +E  +L++LNH N+VRY   W E  ++A+
Sbjct:  497 PNTVQRTGSAMPYQIPT-LALSQSRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPAR--SRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDAD 599          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Fly
Match: Gcn2 (gene:FBgn0019990 transcript:FBtr0334551)

HSP 1 Score: 83.5741 bits (205), Expect = 3.090e-16
Identity = 75/262 (28.63%), Postives = 118/262 (45.04%), Query Frame = 2
Query:  980 SNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS--KFFEFDKCD-------EISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLEL-MVKFSTQSELCHVVEDISK-GIGFKDLIETWPLEDQHVRN---LISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTL 1723
            +N   P M+  +YIQMEFC        C     +     +D   +  L R++ +G+ +IH   +IHRD+KP NIFL  H     Q+KIGDFGL+   F      D       +I+S      T   GT  Y APE   G   +  Y+ K D+Y++G+IL E+    F T  E    +  +    I   D +   P  ++ V+    L+++ P  RP A  +L         +  N L   ++  ++ N + K+   L+ + L
Sbjct:  684 TNSPKPLMQ-VMYIQMEFCEK------CTLRTAIDDNLFNDTDRLWRLFREIAEGLAHIHQQGIIHRDLKPVNIFLDSHD----QIKIGDFGLATTSFLALQAHDAAPAPVNQITSAEDGTGTGKVGTTLYVAPE-LTGNASKSVYNQKVDMYTLGIILFEMCQPPFDTSMERAQTIMALRNVSINIPDAMLKDPKYEKTVKMLQWLLNHDPAQRPTAEELLISDLVPPAQLEANELQEMLRHALA-NPQSKAYKNLVARCL 932          

HSP 2 Score: 55.8398 bits (133), Expect = 1.183e-7
Identity = 31/106 (29.25%), Postives = 55/106 (51.89%), Query Frame = 2
Query:  314 PNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEAN 631
            PN +Q + S +    P       ++L  +F+ +  +GKG FG V    N +D + YAIK++ +     + +      +E  +L++LNH N+VRY   W E  ++A+
Sbjct:  495 PNTVQRTGSAMPYQIPT-LALSQSRLRTEFEVLMYLGKGAFGDVLKVRNILDNREYAIKRIPLPAR--SRQLYKKMTREVELLSRLNHENVVRYFNSWIESVDDAD 597          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Zebrafish
Match: BX072573.1 (eukaryotic translation initiation factor 2-alpha kinase 3 [Source:NCBI gene;Acc:559564])

HSP 1 Score: 139.428 bits (350), Expect = 5.916e-34
Identity = 133/514 (25.88%), Postives = 223/514 (43.39%), Query Frame = 2
Query:  287 KCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYAN---SIP---KIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQ-------------------------NSDCRHSGSNIIFEYSSNFNHKHDSLTSHS----------SEDKGALEICKSSNVNVPKMEFALYIQME--------------FCHLNLQDWLCNRNKLVSTLQDSDFKEVR----CLA--RQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCD-EISSIRKMKL----TANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFK-DLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKE 1627
            +C  D     +  +     V + +P ++   V++ + DF+ ++ +G+GGFG V+ A NK+D   YAIK++ +   +L  +++   ++E + LAKL H  I+RY   W E   +  W   ++       S A+   S P   +           S + S+ M   G + S    +     G    S+   S  D+Q                         + DC  S  +I+FE S   +  +DS  S S          S  K          +++P     L +                 + ++ +Q  LC +  L   +      E+R    CL    Q+ + V+++HS  ++HRD+KP+NIF  +    D  +K+GDFGL    + ++ D E+S++  M +    T   GT  Y +PE  +G     SY HK D+YS+G+IL EL+  F TQ E    + ++ + + F     +   LE Q VR ++S  P  RPEAS I  +   F+E
Sbjct:  577 QCQTDTKFEADVTEPKEVVVTDMRPCEY---VSRYLTDFEPVQCLGRGGFGVVFEARNKVDDCNYAIKRIRLPNRELAREKV---MREVKALAKLEHPGIIRYFNAWQESPPQG-WQEDMDKRWLKDASTADWLLSSPEHMEAFSVKVPVATPSPVDSSCMCAAGGDTSVCVGDQVCVEGLGPDSMM--SERDSQADPDAIMEVSDSPHSFELCPPRTVAHGDCTSSSFDIVFEDSGCEHQDNDSDLSGSTPVPPVCLSQSGSKPQRSTSTGPPISLPSRPTTLSLAPSTPCPAVRPLLTPKVYLYIQMQ--LCRKENLKDWMAQRCLPELREHTQCLDIFLQIAEAVDFLHSKGLMHRDLKPSNIFFTM----DDVVKVGDFGLVTAMDQEEDDEELSTLTPMPIYARHTGQVGTKLYMSPEQLSGN----SYSHKVDIYSLGLILFELLCPFRTQMERVRTLTEV-RALQFPAAFCKANILEAQMVRCMLSRVPAERPEASEI-TEAPLFQE 1069          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Zebrafish
Match: eif2ak3 (eukaryotic translation initiation factor 2-alpha kinase 3 [Source:ZFIN;Acc:ZDB-GENE-050414-2])

HSP 1 Score: 139.428 bits (350), Expect = 6.650e-34
Identity = 133/514 (25.88%), Postives = 223/514 (43.39%), Query Frame = 2
Query:  287 KCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYAN---SIP---KIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQ-------------------------NSDCRHSGSNIIFEYSSNFNHKHDSLTSHS----------SEDKGALEICKSSNVNVPKMEFALYIQME--------------FCHLNLQDWLCNRNKLVSTLQDSDFKEVR----CLA--RQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCD-EISSIRKMKL----TANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFK-DLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKE 1627
            +C  D     +  +     V + +P ++   V++ + DF+ ++ +G+GGFG V+ A NK+D   YAIK++ +   +L  +++   ++E + LAKL H  I+RY   W E   +  W   ++       S A+   S P   +           S + S+ M   G + S    +     G    S+   S  D+Q                         + DC  S  +I+FE S   +  +DS  S S          S  K          +++P     L +                 + ++ +Q  LC +  L   +      E+R    CL    Q+ + V+++HS  ++HRD+KP+NIF  +    D  +K+GDFGL    + ++ D E+S++  M +    T   GT  Y +PE  +G     SY HK D+YS+G+IL EL+  F TQ E    + ++ + + F     +   LE Q VR ++S  P  RPEAS I  +   F+E
Sbjct:  577 QCQTDTKFEADVTEPKEVVVTDMRPCEY---VSRYLTDFEPVQCLGRGGFGVVFEARNKVDDCNYAIKRIRLPNRELAREKV---MREVKALAKLEHPGIIRYFNAWQESPPQG-WQEDMDKRWLKDASTADWLLSSPEHMEAFSVKVPVATPSPVDSSCMCAAGGDTSVCVGDQVCVEGLGPDSMM--SERDSQADPDAIMEVSDSPHSFELCPPRTVAHGDCTSSSFDIVFEDSGCEHQDNDSDLSGSTPVPPVCLSQSGSKPQRSTSTGPPISLPSRPTTLSLAPSTPCPVVRPLLTPKVYLYIQMQ--LCRKENLKDWMAQRCLPELREHTQCLDIFLQIAEAVDFLHSKGLMHRDLKPSNIFFTM----DDVVKVGDFGLVTAMDQEEDDEELSTLTPMPIYARHTGQVGTKLYMSPEQLSGN----SYSHKVDIYSLGLILFELLCPFRTQMERVRTLTEV-RALQFPAAFCKANILEAQMVRCMLSRVPAERPEASEI-TEAPLFQE 1069          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Zebrafish
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:ZFIN;Acc:ZDB-GENE-061013-268])

HSP 1 Score: 129.413 bits (324), Expect = 4.189e-31
Identity = 83/267 (31.09%), Postives = 135/267 (50.56%), Query Frame = 2
Query: 1007 FALYIQMEFCHLNLQDWLCNRNKLVS--------TLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS-KFFEFDKCDEISSIRKMKL------TANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENEL--LRKTLDDLRLSQQKQH 1756
              LYIQM+ C  +L+DW+  RN   +             D ++   + +++L+GVEYIHS  ++HRD+KP NIFL+     +  +KIGDFGL+ +    D+ +++ S  +  +      T+  GT  Y+APE   G      YD KSD+YS+G+I  EL   F T+ E  H + ++ +G     L   WP+  ++++ L S+ P  RP A  +L         +V + L   I +          E E+  LR+ + +L++SQ   H
Sbjct:  366 LMLYIQMQLCERSLKDWIQERNSRTTEGLLMSGDPCDSVDCEQALKILKKILEGVEYIHSCGIMHRDLKPRNIFLL---GNECHVKIGDFGLACQNIIMDEHEKLPSSSQAGVNPDATHTSGVGTFVYAAPEQLEGS----RYDSKSDMYSIGVIAFELFQPFGTEMERVHTLGELRQGKIPNTLSTNWPILAKYIKLLTSSDPSMRPSAPQLLQSDLFSTNDMVIHSLKRKIDE---------QEEEIVQLRRRISELQISQNSIH 616          

HSP 2 Score: 76.6406 bits (187), Expect = 3.825e-14
Identity = 31/87 (35.63%), Postives = 55/87 (63.22%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEA 628
            FQ + ++ +++F+ I  +GKG +G+V+   NK+D Q YA+K+++I   K+   +    ++E ++L+ L H NIV YHT W E  + A
Sbjct:  135 FQAQTSRYLSEFEEIATLGKGSYGKVFRVTNKLDGQEYAVKKILIK--KVTRDDCMKVLREVKVLSSLQHPNIVGYHTAWMEHVQPA 219          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Zebrafish
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:ZFIN;Acc:ZDB-GENE-061013-268])

HSP 1 Score: 129.413 bits (324), Expect = 4.425e-31
Identity = 85/267 (31.84%), Postives = 137/267 (51.31%), Query Frame = 2
Query: 1007 FALYIQMEFCHLNLQDWLCNRNK------LVS--TLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS-KFFEFDKCDEISSIRKMKL------TANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENEL--LRKTLDDLRLSQQKQH 1756
              LYIQM+ C  +L+DW+  RN       L+S       D ++   + +++L+GVEYIHS  ++HRD+KP NIFL+     +  +KIGDFGL+ +    D+ +++ S  +  +      T+  GT  Y+APE   G      YD KSD+YS+G+I  EL   F T+ E  H + ++ +G     L   WP+  ++++ L S+ P  RP A  +L         +V + L   I +          E E+  LR+ + +L++SQ   H
Sbjct:  366 LMLYIQMQLCERSLKDWIQERNSRTTEGLLISGDPCDSVDCEQALKILKKILEGVEYIHSCGIMHRDLKPRNIFLL---GNECHVKIGDFGLACQNIIMDEHEKLPSSSQAGVNPDATHTSGVGTFVYAAPEQLEGS----RYDSKSDMYSIGVIAFELFQPFGTEMERVHTLGELRQGKIPNTLSTNWPILAKYIKLLTSSDPSMRPSAPQLLQSDLFSTNDMVIHSLKRKIDE---------QEEEIVQLRRRISELQISQNSIH 616          

HSP 2 Score: 76.6406 bits (187), Expect = 4.137e-14
Identity = 31/87 (35.63%), Postives = 55/87 (63.22%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEA 628
            FQ + ++ +++F+ I  +GKG +G+V+   NK+D Q YA+K+++I   K+   +    ++E ++L+ L H NIV YHT W E  + A
Sbjct:  135 FQAQTSRYLSEFEEIATLGKGSYGKVFRVTNKLDGQEYAVKKILIK--KVTRDDCMKVLREVKVLSSLQHPNIVGYHTAWMEHVQPA 219          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Zebrafish
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:ZFIN;Acc:ZDB-GENE-061013-268])

HSP 1 Score: 126.331 bits (316), Expect = 6.343e-30
Identity = 81/267 (30.34%), Postives = 135/267 (50.56%), Query Frame = 2
Query: 1007 FALYIQMEFCHLNLQDWLCNRNKLVS--------TLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS-KFFEFDKCDEISSIRKMKL------TANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENEL--LRKTLDDLRLSQQKQH 1756
              LYIQM+ C  +L+DW+  RN   +             D ++   + +++L+GVEYIHS  ++HRD+KP NIFL+     +  +KIGDFGL+ +    D+ +++ S  +  +      T+  GT  Y+APE   G      YD K+++YS+G+I  EL   F T+ E  H + ++ +G     L   WP+  ++++ L S+ P  RP A  +L         +V + L   I +          E E+  LR+ + +L++SQ   H
Sbjct:  413 LMLYIQMQLCERSLKDWIQERNSRTTEGLLMSGDPCDSVDCEQALKILKKILEGVEYIHSCGIMHRDLKPRNIFLL---GNECHVKIGDFGLACQNIIMDEHEKLPSSSQAGVNPDATHTSGVGTFVYAAPEQLEGS----RYDSKANMYSIGVIAFELFQPFGTEMERVHTLGELRQGKIPNTLSTNWPILAKYIKLLTSSDPSMRPSAPQLLQSDLFSTNDMVIHSLKRKIDE---------QEEEIVQLRRRISELQISQNSIH 663          

HSP 2 Score: 76.6406 bits (187), Expect = 4.375e-14
Identity = 31/87 (35.63%), Postives = 55/87 (63.22%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEA 628
            FQ + ++ +++F+ I  +GKG +G+V+   NK+D Q YA+K+++I   K+   +    ++E ++L+ L H NIV YHT W E  + A
Sbjct:  182 FQAQTSRYLSEFEEIATLGKGSYGKVFRVTNKLDGQEYAVKKILIK--KVTRDDCMKVLREVKVLSSLQHPNIVGYHTAWMEHVQPA 266          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Xenopus
Match: eif2ak1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:NCBI gene;Acc:733794])

HSP 1 Score: 193.356 bits (490), Expect = 4.060e-53
Identity = 148/481 (30.77%), Postives = 235/481 (48.86%), Query Frame = 2
Query:  377 KVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQ----VENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSI------YDR---SNIDTQNSDCRHS--GSNIIFEYSS-NFNHKHDSLTSHSS--EDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDS---DFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS--KFFEFDKCDEISSIRKMKLTANT--GTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLR 1735
            + ++ +N+FD I  +GKGG+G+VY   NK+D Q YAIK+++I   K++ ++    ++E ++LA L H NIV YHT W E  +  N   +    V+ F N        + ++    S  +      S     +  +VS   N S  +     H I      Y +   +N+  +   C  S     ++ +     F+ + D   S SS  E+    ++C   +  + +    L+IQM+ C  +L DW+  RN       +S   DF +VR   R   Q+L+GV YIHS  V+HRD+KP N+FL      D+ ++IGDFGL+    F+  +   +   R   LT  T  GT  Y+APE   G      YD KSD+YS+G+ILLEL   F T+ E   V+  +  G         WP++   V+ L S++   RP AS +L       ES + +   N ++D+  +   L+ ENE L+++L  L+
Sbjct:  158 QTSRYLNEFDEIAQLGKGGYGKVYKVRNKLDGQFYAIKKILIK--KVSRRDCRKVLREVKVLAGLQHPNIVGYHTAWMEHVQPPNSQCKLLKAVKGFPNNKAE--QDMKEVRSVESSGSFIMFEDSAHQDPSAQSVSESVNMSVSSPDKPDHCIENAGNNYKKDSNANVAKKTKACSKSYQSQGLVCQRDKCPFSSEDDVDKSDSSCDEECPQKDLCLHRHFEI-QYHLMLHIQMKLCEKSLWDWIVERNNHFQESGNSNPYDFVDVRITTRILYQILEGVRYIHSMEVLHRDLKPRNVFL---HGADLHVRIGDFGLACRDIFQQSRDSWLQKDRSNGLTHTTGVGTCLYAAPEQLKGS----HYDFKSDMYSIGIILLELFQPFGTEMERTQVLTALRNGTVPCSYERQWPVQSNFVKLLTSSECSERPSASQML-------ESQLFHQKENLVQDLQKKVLYLEEENERLKRSLQKLQ 619          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Xenopus
Match: eif2ak1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:NCBI gene;Acc:733794])

HSP 1 Score: 161.384 bits (407), Expect = 7.571e-42
Identity = 138/481 (28.69%), Postives = 217/481 (45.11%), Query Frame = 2
Query:  377 KVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQ----VENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSI------YDR---SNIDTQNSDCRHS--GSNIIFEYSS-NFNHKHDSLTSHSS--EDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDS---DFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS--KFFEFDKCDEISSIRKMKLTANT--GTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLR 1735
            + ++ +N+FD I  +GKGG+G+VY                               ++E ++LA L H NIV YHT W E  +  N   +    V+ F N        + ++    S  +      S     +  +VS   N S  +     H I      Y +   +N+  +   C  S     ++ +     F+ + D   S SS  E+    ++C   +  + +    L+IQM+ C  +L DW+  RN       +S   DF +VR   R   Q+L+GV YIHS  V+HRD+KP N+FL      D+ ++IGDFGL+    F+  +   +   R   LT  T  GT  Y+APE   G      YD KSD+YS+G+ILLEL   F T+ E   V+  +  G         WP++   V+ L S++   RP AS +L       ES + +   N ++D+  +   L+ ENE L+++L  L+
Sbjct:  158 QTSRYLNEFDEIAQLGKGGYGKVYK-----------------------------VLREVKVLAGLQHPNIVGYHTAWMEHVQPPNSQCKLLKAVKGFPNNKAE--QDMKEVRSVESSGSFIMFEDSAHQDPSAQSVSESVNMSVSSPDKPDHCIENAGNNYKKDSNANVAKKTKACSKSYQSQGLVCQRDKCPFSSEDDVDKSDSSCDEECPQKDLCLHRHFEI-QYHLMLHIQMKLCEKSLWDWIVERNNHFQESGNSNPYDFVDVRITTRILYQILEGVRYIHSMEVLHRDLKPRNVFLH---GADLHVRIGDFGLACRDIFQQSRDSWLQKDRSNGLTHTTGVGTCLYAAPEQLKGS----HYDFKSDMYSIGIILLELFQPFGTEMERTQVLTALRNGTVPCSYERQWPVQSNFVKLLTSSECSERPSASQML-------ESQLFHQKENLVQDLQKKVLYLEEENERLKRSLQKLQ 592          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Xenopus
Match: cpt1al (carnitine palmitoyltransferase 1A (liver) like [Source:Xenbase;Acc:XB-GENE-5770297])

HSP 1 Score: 152.91 bits (385), Expect = 2.557e-38
Identity = 135/489 (27.61%), Postives = 226/489 (46.22%), Query Frame = 2
Query:  380 VNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRH-------------------SGSNIIFEYSS---NFNH----------------KHDSLTSHSSEDKGA----------------LEICKSSNVNVPKMEFALY--IQMEFCHL-NLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKM----KLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLI-ETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHI 1660
            V++ + DF+ I+ +G+GGFG V+ A NK+D   YAIK++ +   +L  +++   ++E + LAKL H  IVRY   W E   E  W  +++      GS    +   +     +     +   SM      V  +T + +++  SLS +      I   + D  H                   + S+I+FE S    + NH                + +S T  S+E K                  L++ K++  ++      +Y  IQM+ C   NL+DW+  R     TL++    E   +  Q+ + V+++HS  ++HRD+KP+NIF  +    D  +K+GDFGL    + ++ DE + +  M    + T   GT  Y +PE   G+    SY HK D++S+G+IL EL+  FSTQ E   V+ ++ + + F  L  E +  E   V ++IS+ P  RPEA           E I++N L++ I
Sbjct:  399 VSRYLTDFEPIQCLGRGGFGVVFEARNKVDDCNYAIKRIRLPNRELAREKV---MREVKALAKLEHPGIVRYFNAWLEAPPEL-WQEKMDELLLKDGSTDWPLSSPTPLDGLSVKVRSVDPYSMK---EQVEVITPSESYS--SLSAAFVAVPGIGDSDDDDNHGIWYEHCPRTAADIGVHERTSSSIVFEDSGCDNSSNHVDKAEVSVLVMNSEFSERESGTKDSNESKPTSISTLSESPPRPKSLCLDLSKNTAESIKPTSPKVYLYIQMQLCRKENLKDWMSAR----CTLEERPQTESLHVFLQIAEAVQFLHSKGLMHRDLKPSNIFFTL----DDVVKVGDFGLVTAMDQEE-DEEAVLTPMPAYARHTGQVGTKLYMSPEQIFGQ----SYSHKVDIFSLGLILFELLYPFSTQMERVRVLTEL-RDLKFPQLFTERYSTEQNMVLHMISHNPNERPEA-----------EEIIENPLFDSI 853          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Xenopus
Match: cpt1al (carnitine palmitoyltransferase 1A (liver) like [Source:Xenbase;Acc:XB-GENE-5770297])

HSP 1 Score: 148.673 bits (374), Expect = 6.621e-37
Identity = 134/489 (27.40%), Postives = 226/489 (46.22%), Query Frame = 2
Query:  380 VNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRH-------------------SGSNIIFEYSS---NFNH----------------KHDSLTSHSSEDKGA----------------LEICKSSNVNVPKMEFALY--IQMEFCHL-NLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKM----KLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLI-ETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHI 1660
            V++ + DF+ I+ +G+GGFG V+ A NK+D   YAIK++ +   +L  +++   ++E + LAKL H  IVRY   W E   E  W  +++      GS    +   +     +     +   SM      V  +T + +++  SLS +      I   + D  H                   + S+I+FE S    + NH                + +S T  S+E K                  L++ K++  ++      +Y  IQM+ C   NL+DW+  R     TL++    E   +  Q+ + V+++HS  ++HRD+KP+NIF  +    D  +K+GDFGL    + ++ DE + +  M    + T   GT  Y +PE   G+    SY HK D++S+G+IL EL+  FSTQ E   V+ ++ + + F  L+     L+   V ++IS+ P  RPEA           E I++N L++ I
Sbjct:  397 VSRYLTDFEPIQCLGRGGFGVVFEARNKVDDCNYAIKRIRLPNRELAREKV---MREVKALAKLEHPGIVRYFNAWLEAPPEL-WQEKMDELLLKDGSTDWPLSSPTPLDGLSVKVRSVDPYSMK---EQVEVITPSESYS--SLSAAFVAVPGIGDSDDDDNHGIWYEHCPRTAADIGVHERTSSSIVFEDSGCDNSSNHVDKAEVSVLVMNSEFSERESGTKDSNESKPTSISTLSESPPRPKSLCLDLSKNTAESIKPTSPKVYLYIQMQLCRKENLKDWMSAR----CTLEERPQTESLHVFLQIAEAVQFLHSKGLMHRDLKPSNIFFTL----DDVVKVGDFGLVTAMDQEE-DEEAVLTPMPAYARHTGQVGTKLYMSPEQIFGQ----SYSHKVDIFSLGLILFELLYPFSTQMERVRVLTEL-RDLKFPQLLFLCLYLQQNMVLHMISHNPNERPEA-----------EEIIENPLFDSI 851          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Xenopus
Match: eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:100216307])

HSP 1 Score: 134.035 bits (336), Expect = 5.670e-32
Identity = 139/455 (30.55%), Postives = 205/455 (45.05%), Query Frame = 2
Query:  362 IKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSY-----------------ANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDS-LTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDS-------DFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGL----------SKFFEFDKC--DEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVK-FSTQSELCHVVEDISKG-IGFKDLIETWPLEDQH--VRNLISNKPENRPEASFIL 1603
            I+ Q + ++  N+F+ ++ +GKG FG V    NK+D   YA+K+V IN +    K+      E  +L++LNH NIVRY+  W E+ E    + + E       +                   N+   I  SS   + S    S++   NG   SS  ++     G  S S    ++ D          S IIFE   + +   D     ++ ED+ A      +N  V +    LYIQME+C               STL+D+       D   +  L R++L G+ YIH   +IHRD+KP NIFL    S+D  +KIGDFGL          S   E D+   D +    + KLT   GTA Y +PEV  G  K  SY+ K DLYS+G+IL E+  +   T SE   V+  + +  I F    E    E Q   +  L+++ P +RP A  +L
Sbjct:  594 IESQRQFSRYFNEFEELQMLGKGAFGAVIKVQNKLDGCYYAVKRVQINPAS---KQFRRIKGEVTLLSRLNHENIVRYYNAWIEKHEHPTISPKCEAHEEKKVTVKPEQIVKNDLGLLDDIEENAPAPILTSSVEWSTSCERSSSAHCTNGDQDSSDDDDDDDEEGVFSPSFLPGTDSD----------SEIIFENEDDISPDQDEEYDKNNVEDRKA-----GANEIVTQAVHYLYIQMEYCE-------------KSTLRDTIDQGLYMDVGRLWRLFREILDGLTYIHEQGMIHRDLKPVNIFL---DSED-HVKIGDFGLATDHPASYTESAQGEEDQAHSDLVKVDPEGKLTGMVGTALYVSPEV-QGNTK-ASYNQKVDLYSLGIILFEMSYRPMDTSSERISVLSRLRQALIVFPSDFENPENEKQKKVITWLLNHDPASRPTAMELL 1011          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Mouse
Match: Eif2ak1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:MGI Symbol;Acc:MGI:1353448])

HSP 1 Score: 170.629 bits (431), Expect = 3.283e-45
Identity = 133/449 (29.62%), Postives = 209/449 (46.55%), Query Frame = 2
Query:  338 SFVQNFKP--IKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQV-------ENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSL--SHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHD---SLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRC----------LARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLT--ANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILN 1606
            S++Q  +   I F+ + ++ +N+F+ +  +GKGG+GRVY   NK+D Q YAIK+++I  +   + +    ++E ++LA L H NIV YHT W E         +V       E  +   G       K + SSS    + +       F  S V +  NN    T +L   +S    S+I+ Q           +  +     H  +   + TS     +G L +   + V   +    L+IQM+ C L+L DW+  RNK      D    E  C          + ++L++GV YIH+  ++HRD+KP NIFL      D Q+KIGDFGL+        D  +   K   T  +  GT  Y++PE   G      YD KSD+YS+G+ILLEL   F T+ E   V+  +  G   + L +  P++ ++++ L       RP A  +L 
Sbjct:  144 SYMQKIRSREIAFEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSA--TKTDCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVVQPQDRVPIQLPSLEVLSEQEGDRDQGGVKDNESSSSIVFAELTPEKEKPFGESEVKNENNNLVSYTANLVVRNSSESESSIELQEDGLTDLSVRPVVRHQLPLGHSSELEGNFTSTDESSEGNLNLLGQTEV---RYHLMLHIQMQLCELSLWDWITERNKRSREYVD----EAACPYVMASVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFL---HGPDQQVKIGDFGLACADIIQNADWTNRNGKGTRTHTSRVGTCLYASPEQLEGS----QYDAKSDMYSLGVILLELFQPFGTEMERATVLTGVRTGRIPESLSKRCPVQAKYIQLLTGRNVSQRPSALQLLQ 576          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Mouse
Match: Eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:MGI Symbol;Acc:MGI:1353427])

HSP 1 Score: 126.716 bits (317), Expect = 8.562e-30
Identity = 127/418 (30.38%), Postives = 189/418 (45.22%), Query Frame = 2
Query:  296 GDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEA--------NWNSQVENF----------TNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSF--NGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSED----KGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS----KFFEFDKCDE------ISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLEL 1438
            G ++I      N +     F   + QF  ++   +F+ ++ +GKG FG V    NK+D   YA+K++ IN +  + + I     E  +L++L+H NIVRY+  W ER E          +   Q ++           T   GS   + P    SSS   ++S   STS  F   G + SS   +     G  S S    S+ D          S+IIF+     N   +S + +  ED     G+ EI    +V    + + LYIQME+C          ++ L  T+    F++   L R   ++L G+ YIH   +IHRD+KP NIFL      D  +KIGDFGL+     F    K D+      I S     LT   GTA Y +PEV  G  K  +Y+ K DL+S+G+I  E+
Sbjct:  278 GQDYIETVIPSNQLPSAAFFSETQKQF--SRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKG---EVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSD----------SDIIFD-----NEDENSKSQNQDEDCNQKDGSHEI--EPSVTAEAVHY-LYIQMEYC---------EKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFL----DSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEV-QGSTKS-AYNQKVDLFSLGIIFFEM 657          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Mouse
Match: Eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:MGI Symbol;Acc:MGI:1353427])

HSP 1 Score: 126.716 bits (317), Expect = 9.343e-30
Identity = 127/418 (30.38%), Postives = 189/418 (45.22%), Query Frame = 2
Query:  296 GDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEA--------NWNSQVENF----------TNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSF--NGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSED----KGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS----KFFEFDKCDE------ISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLEL 1438
            G ++I      N +     F   + QF  ++   +F+ ++ +GKG FG V    NK+D   YA+K++ IN +  + + I     E  +L++L+H NIVRY+  W ER E          +   Q ++           T   GS   + P    SSS   ++S   STS  F   G + SS   +     G  S S    S+ D          S+IIF+     N   +S + +  ED     G+ EI    +V    + + LYIQME+C          ++ L  T+    F++   L R   ++L G+ YIH   +IHRD+KP NIFL      D  +KIGDFGL+     F    K D+      I S     LT   GTA Y +PEV  G  K  +Y+ K DL+S+G+I  E+
Sbjct:  478 GQDYIETVIPSNQLPSAAFFSETQKQF--SRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKG---EVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSD----------SDIIFD-----NEDENSKSQNQDEDCNQKDGSHEI--EPSVTAEAVHY-LYIQMEYC---------EKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFL----DSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEV-QGSTKS-AYNQKVDLFSLGIIFFEM 857          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Mouse
Match: Eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:MGI Symbol;Acc:MGI:1353427])

HSP 1 Score: 126.716 bits (317), Expect = 9.521e-30
Identity = 125/414 (30.19%), Postives = 189/414 (45.65%), Query Frame = 2
Query:  296 GDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEA--------NWNSQVENF----------TNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSF--NGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS----KFFEFDKCDE------ISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLEL 1438
            G ++I      N +     F   + QF  ++   +F+ ++ +GKG FG V    NK+D   YA+K++ IN +  + + I     E  +L++L+H NIVRY+  W ER E          +   Q ++           T   GS   + P    SSS   ++S   STS  F   G + SS   +     G  S S    S+ D          S+IIF+ + + N K  +     ++  G+ EI    +V    + + LYIQME+C          ++ L  T+    F++   L R   ++L G+ YIH   +IHRD+KP NIFL      D  +KIGDFGL+     F    K D+      I S     LT   GTA Y +PEV  G  K  +Y+ K DL+S+G+I  E+
Sbjct:  556 GQDYIETVIPSNQLPSAAFFSETQKQF--SRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKG---EVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSD----------SDIIFD-NEDENSKSQNQDEDCNQKDGSHEI--EPSVTAEAVHY-LYIQMEYC---------EKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFL----DSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEV-QGSTKS-AYNQKVDLFSLGIIFFEM 935          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Mouse
Match: Eif2ak4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:MGI Symbol;Acc:MGI:1353427])

HSP 1 Score: 126.716 bits (317), Expect = 9.831e-30
Identity = 127/418 (30.38%), Postives = 189/418 (45.22%), Query Frame = 2
Query:  296 GDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEA--------NWNSQVENF----------TNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSF--NGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSED----KGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS----KFFEFDKCDE------ISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLEL 1438
            G ++I      N +     F   + QF  ++   +F+ ++ +GKG FG V    NK+D   YA+K++ IN +  + + I     E  +L++L+H NIVRY+  W ER E          +   Q ++           T   GS   + P    SSS   ++S   STS  F   G + SS   +     G  S S    S+ D          S+IIF+     N   +S + +  ED     G+ EI    +V    + + LYIQME+C          ++ L  T+    F++   L R   ++L G+ YIH   +IHRD+KP NIFL      D  +KIGDFGL+     F    K D+      I S     LT   GTA Y +PEV  G  K  +Y+ K DL+S+G+I  E+
Sbjct:  435 GQDYIETVIPSNQLPSAAFFSETQKQF--SRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKG---EVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSD----------SDIIFD-----NEDENSKSQNQDEDCNQKDGSHEI--EPSVTAEAVHY-LYIQMEYC---------EKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFL----DSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEV-QGSTKS-AYNQKVDLFSLGIIFFEM 814          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. UniProt/SwissProt
Match: sp|Q4R8E0|E2AK1_MACFA (Eukaryotic translation initiation factor 2-alpha kinase 1 OS=Macaca fascicularis OX=9541 GN=EIF2AK1 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.647e-44
Identity = 142/489 (29.04%), Postives = 219/489 (44.79%), Query Frame = 2
Query:  335 ISFVQNFK--PIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERF-----------------------EEANWNSQVENFTNCSGS--YANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSL--SHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNF---NHKHDSLTSHSSEDKGALEICKSSNVNV-----PKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRC----------LARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEI----------SSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKES 1630
            +S +Q  +   + F+ + ++ +N+F+ +  +GKGG+GRVY   NK+D Q YAIK+++I  +   + +    ++E ++LA L H NIV YHT W E                         EE      V+N  + S S  +A   P+                    F  S+  +  + S   T SL    S    S ++ Q +D     ++ I E        +H  DS TS         E     NVN       +    L+IQM+ C L+L DW+  RNK      D    E  C          + ++L++GV YIH+  ++HRD+KP NIFL  H   D Q+KIGDFGL+       C +I          +  R    T+  GT  Y++PE   G      YD KSD+YS+G+ILLEL   F T+ E   V+  +  G   + L +  P++ +++++L       RP A  +L Q+  F+ S
Sbjct:  143 VSHIQKIRSREVAFEAQTSRYLNEFEEVAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGA--TKTDCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRADRAAIELPTLEVLSDQEEDREQYDVKNDESSSSSIVFAEPTPE----------------KGKRFGESDTENQNDKSVKYTTSLVIRDSGELESTLELQENDLAGLSTSSIMEQQLPLRRNSHLDDSFTS--------TEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIAERNKRSRECVD----ESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFL--H-GPDQQVKIGDFGLA-------CTDILQKNADWTNRNGKRTPTHTSRVGTCLYASPEQLEGS----EYDAKSDMYSLGVILLELFQPFGTEMERAEVLTGLRTGQLPESLSKRCPVQAKYIQHLTRRNSSQRPSAVQLL-QSELFQTS 586          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. UniProt/SwissProt
Match: sp|Q9Z2R9|E2AK1_MOUSE (Eukaryotic translation initiation factor 2-alpha kinase 1 OS=Mus musculus OX=10090 GN=Eif2ak1 PE=1 SV=2)

HSP 1 Score: 170.629 bits (431), Expect = 2.297e-44
Identity = 133/449 (29.62%), Postives = 209/449 (46.55%), Query Frame = 2
Query:  338 SFVQNFKP--IKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQV-------ENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSL--SHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHD---SLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRC----------LARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLT--ANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILN 1606
            S++Q  +   I F+ + ++ +N+F+ +  +GKGG+GRVY   NK+D Q YAIK+++I  +   + +    ++E ++LA L H NIV YHT W E         +V       E  +   G       K + SSS    + +       F  S V +  NN    T +L   +S    S+I+ Q           +  +     H  +   + TS     +G L +   + V   +    L+IQM+ C L+L DW+  RNK      D    E  C          + ++L++GV YIH+  ++HRD+KP NIFL      D Q+KIGDFGL+        D  +   K   T  +  GT  Y++PE   G      YD KSD+YS+G+ILLEL   F T+ E   V+  +  G   + L +  P++ ++++ L       RP A  +L 
Sbjct:  144 SYMQKIRSREIAFEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSA--TKTDCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVVQPQDRVPIQLPSLEVLSEQEGDRDQGGVKDNESSSSIVFAELTPEKEKPFGESEVKNENNNLVSYTANLVVRNSSESESSIELQEDGLTDLSVRPVVRHQLPLGHSSELEGNFTSTDESSEGNLNLLGQTEV---RYHLMLHIQMQLCELSLWDWITERNKRSREYVD----EAACPYVMASVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFL---HGPDQQVKIGDFGLACADIIQNADWTNRNGKGTRTHTSRVGTCLYASPEQLEGS----QYDAKSDMYSLGVILLELFQPFGTEMERATVLTGVRTGRIPESLSKRCPVQAKYIQLLTGRNVSQRPSALQLLQ 576          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. UniProt/SwissProt
Match: sp|Q9BQI3|E2AK1_HUMAN (Eukaryotic translation initiation factor 2-alpha kinase 1 OS=Homo sapiens OX=9606 GN=EIF2AK1 PE=1 SV=2)

HSP 1 Score: 165.236 bits (417), Expect = 2.391e-42
Identity = 139/478 (29.08%), Postives = 218/478 (45.61%), Query Frame = 2
Query:  335 ISFVQNFK--PIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFE-------RFEEAN--------WNSQVENFTNCS----GSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNV-----PKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRC----------LARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEI----------SSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKES 1630
            IS +Q  +   +  + + ++ +N+F+ +  +GKGG+GRVY   NK+D Q YAIK+++I  +   +      ++E ++LA L H NIV YHT W E       R + A          + Q E+   C      S ++SI  I    +         S + + N  +V   TN     +G L       S ++ Q +      ++ I E             SH  E   + E     NVN       +    L+IQM+ C L+L DW+  RNK      D    E  C          + ++L++GV YIH+  ++HRD+KP NIFL  H   D Q+KIGDFGL+       C +I          +  R    T+  GT  Y++PE   G      YD KSD+YS+G++LLEL   F T+ E   V+  +  G   + L +  P++ +++++L       RP A  +L Q+  F+ S
Sbjct:  143 ISRIQKIRSREVALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQYYAIKKILIKGA--TKTVCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVIQPRADRAAIELPSLEVLSDQEEDREQCGVKNDESSSSSI--IFAEPTPEKEKRFGESDTENQNNKSVKYTTNLVIRESGELE------STLELQENGLAGLSASSIVEQQLPLRR-----NSHLEESFTSTEESSEENVNFLGQTEAQYHLMLHIQMQLCELSLWDWIVERNKRGREYVD----ESACPYVMANVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFL--H-GPDQQVKIGDFGLA-------CTDILQKNTDWTNRNGKRTPTHTSRVGTCLYASPEQLEGS----EYDAKSDMYSLGVVLLELFQPFGTEMERAEVLTGLRTGQLPESLRKRCPVQAKYIQHLTRRNSSQRPSAIQLL-QSELFQNS 586          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. UniProt/SwissProt
Match: sp|Q63185|E2AK1_RAT (Eukaryotic translation initiation factor 2-alpha kinase 1 OS=Rattus norvegicus OX=10116 GN=Eif2ak1 PE=1 SV=2)

HSP 1 Score: 162.155 bits (409), Expect = 2.442e-41
Identity = 130/448 (29.02%), Postives = 209/448 (46.65%), Query Frame = 2
Query:  338 SFVQNFK--PIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQV-------ENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSL--SHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRC----------LARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMK----LTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILN 1606
            S++Q  +   I  + + ++ +N+F+ +  +GKGG+GRVY   NK+D Q YAIK+++I  +   + +    ++E ++LA L H NIV YHT W E         +V       E  +   G       K + SSS    + +          S+V +  NN      +L    S    S+I+ Q      S    + ++     H  D   + +S D+ + +          +    L+IQM+ C L+L DW+  RN       D    E  C          + ++L++GV YIH+  ++HRD+KP NIFL  H   D Q+KIGDFGL+      K  + ++ R  K     T+  GT  Y++PE   G      YD KSD+YS+G+ILLEL   F T+ E   V+  +  G   + L +  P++ ++++ L       RP A  +L 
Sbjct:  144 SYMQKIRSREIALEAQTSRYLNEFEELAILGKGGYGRVYKVRNKLDGQHYAIKKILIKSA--TKTDCMKVLREVKVLAGLQHPNIVGYHTAWIEHVHVLQPQDRVPIQLPSLEVLSEHEGDRNQGGVKDNESSSSIIFAELTPEKENPLAESDVKNENNNLVSYRANLVIRSSSESESSIELQEDGLNESPLRPVVKHQLPLGHSSDVEGNFTSTDESSEDNLNLLGQTEARYHLMLHIQMQLCELSLWDWIAERNNRSRECVD----EAACPYVMASVATKIFQELVEGVFYIHNMGIVHRDLKPRNIFL--H-GPDQQVKIGDFGLACADIIQKSADWTN-RNGKGTPTHTSRVGTCLYASPEQLEGS----EYDAKSDMYSLGVILLELFQPFGTEMERATVLTGVRTGRIPESLSKRCPVQAKYIQLLTGRNAAQRPSALQLLQ 577          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. UniProt/SwissProt
Match: sp|Q9HGN1|GCN2_SCHPO (eIF-2-alpha kinase GCN2 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gcn2 PE=2 SV=2)

HSP 1 Score: 148.673 bits (374), Expect = 5.287e-36
Identity = 132/448 (29.46%), Postives = 215/448 (47.99%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCH-LNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS-----------KFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDI-SKGIGFKDLIETWPL-----EDQHVRNLISNKPENRPEASFILNQTCC---FKESIVQNGL 1648
            ++  V++   DF+ ++ +G+GGFG V    N+ID + YA+K++++     ++KE +  ++E   L++L+H ++VRY+T W E   EAN                +++ +I  S S + + S     +M+ +    SSL    A    SL     D  N      D     S+I F+YS+  + +       SS+   ++E   S       +   LYIQME+C  L+LQD +  R+K+          E+  L RQ+L+ + YIHS  ++HRD+KP NIFL     ++  +K+GDFGL+           K+      DE        LT   GTA Y APE+ + +   + YD K D+YS+G+IL E+ + FST  E   +++ I S  I F     T+P      E + +  L+ + P  RP +  +L          E  +Q GL
Sbjct:  545 YRKSVSRYETDFEELEFLGRGGFGEVVKVKNRIDGRFYAVKKLVLLS---DDKENSRILREVMTLSRLHHEHVVRYYTAWVE--TEAN----------------DTVTEIISSDSESLSQS----LNMAVDFRQSSSL---PADKLSSLDIHFEDDYNSSADEEDP--EASDISFQYSNTSDKEG------SSDKDSSIEEASSVKTQENGLNATLYIQMEYCEKLSLQDII--RDKI-------PVDEMWRLFRQILEALAYIHSRGMMHRDLKPGNIFL----DENRNVKLGDFGLATENENYQDNNDKWKNRQSADE-------DLTTGVGTALYVAPELLSRR-NGVRYDAKVDMYSLGIILFEMCMTFSTSMERIRIIDTIRSPSISFPS---TFPFSRASHEFKVIHCLLQHDPTKRPSSQELLESEAIPPKVGEEFIQEGL 932          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. TrEMBL
Match: A0A1L8EXZ4 (Protein kinase domain-containing protein OS=Xenopus laevis OX=8355 GN=eif2ak1 PE=1 SV=1)

HSP 1 Score: 197.208 bits (500), Expect = 2.938e-51
Identity = 160/515 (31.07%), Postives = 246/515 (47.77%), Query Frame = 2
Query:  335 ISF-VQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVS---SLTNNSAFNTGSLS------------HSIYDRSNIDTQNSD---------CRHS--GSNIIFEYSSNFNHKHDSLTSHSS--EDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDS---DFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEI-----------SSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFIL-NQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLRL 1738
            +SF V   K +  + + ++ +N+FD I  +GKGG+G+VY   NK+D Q YAIK+++I   K++ ++    ++E ++LA L H NIV YHT W E  +  N  SQ +      GS  N   +            +    S+  +GS ++   S+  +    TGS S            HSI + +N   ++S+         C  S     +       FN + D   S SS  E+    ++C   +  + +    L+IQM+ C  +L DW+  RN       +S   DF +VR   R   Q+L+GV Y+HS  V+HRD+KP N+FL      D+ ++IGDFGL+       C +I              +    T   GT  Y+APE   G      YD KSD+YS+G+ILLEL   F T+ E   V+  +  G         WP++ + V+ L S++   RP AS +L +Q    KE+IVQ        D+  +   L+ ENE L+K+L  L+L
Sbjct:  143 LSFHVSRAKKMLSETQTSRYLNEFDEIVQLGKGGYGKVYKVRNKLDGQFYAIKKILIK--KVSRRDCKKVLREVKVLAGLQHPNIVGYHTAWMEHVQPPN--SQCKLLKVIKGSSDNKAEQ-----------DMKGVQSVESSGSFITFEDSVEGDPGEQTGSESVNMSLSSLNKREHSIENAANNYRKDSNTNVTRKTRVCHKSYQSPGLCQRDECPFNSEEDVDKSDSSCDEECPQKDLCLHRHFEI-QYHLMLHIQMKLCEKSLWDWIVERNNHFQESGNSNPYDFVDVRITTRILYQILEGVRYVHSMGVLHRDLKPRNVFL---HGADLHVRIGDFGLA-------CRDIIQKSRDSWLNKDGSKGATHTTGVGTCLYAAPEQLKGS----RYDFKSDMYSIGIILLELFQPFGTEMERTRVLTALRNGTVPGSYERQWPVQSEFVKLLTSSECSERPSASQMLESQLFHQKENIVQ--------DLQKKVLDLEEENERLKKSLQKLQL 619          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. TrEMBL
Match: A0A099ZQU1 (Eukaryotic translation initiation factor 2-alpha kinase 1 (Fragment) OS=Tinamus guttatus OX=94827 GN=N309_06018 PE=4 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 7.592e-51
Identity = 141/455 (30.99%), Postives = 222/455 (48.79%), Query Frame = 2
Query:  356 KPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYAN-----------SIPKIXXXXXXXXXXXXXXXTSM-SFNGSNVSSLTNNSAFNTGS-----------LSHSI--YDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGA----LEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKE---------VRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS----KFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEAS 1594
            K   F+ + ++ +N+FD I  +GKGG+G+VY   NK+D Q YAIK+  IN  K   ++    ++E ++LA L H NIV YHT W E  + A     +      S    N           S   I  +   +        TS+ + N  ++ S+   + F + S           LS  +  Y  +++D+ +SD ++  S     Y  +F+  +DS+    S  +G+    + +C +  V    M   LYIQM+ C ++L DW+  RNK  S   +  F +         ++C  ++LL+GV YIHS  V+HRD+KP NIFL      D  +KIGDFGL+     + + D   + + I  +  T+  GT  Y++PE   G      YD KSD+YS+G+ILLEL   F T+ E   V+  +  G       + WP++ ++V+ L S  P  RP A+
Sbjct:  112 KEFLFESQTSRYLNEFDEIARLGKGGYGKVYKVRNKLDGQFYAIKR--INIKKATRRDCMKVLREVKVLAGLQHPNIVGYHTAWMEHAQLACPKKAIMTLEPLSLEQENGNDHRHIQSVESDSSIIFADLTSQEKKSRDCTSLKNLNNESMPSMDEGNDFTSSSNKIYTKPDKCKLSVELQEYSTAHVDSTSSDFKNHSS-----YGPHFSLDNDSIAGSKSCIEGSSQTDIALCGAFEVEYRLM---LYIQMQLCEISLCDWIAERNKRCSERAEETFSQYHLVDVTWTMKCF-QELLEGVCYIHSMGVMHRDLKPRNIFLY---GSDRHVKIGDFGLACKDLLWHDADHWLKTNRINGLTHTSGVGTCLYASPEQLQGS----HYDFKSDMYSMGVILLELFQPFGTEMERTEVLMCLRNGHIPNTFYKKWPIQAKYVKLLTSKIPSQRPTAA 548          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. TrEMBL
Match: Q28EX6 (Eukaryotic translation initiation factor 2 alpha kinase 1 OS=Xenopus tropicalis OX=8364 GN=eif2ak1 PE=2 SV=1)

HSP 1 Score: 195.282 bits (495), Expect = 1.670e-50
Identity = 150/481 (31.19%), Postives = 236/481 (49.06%), Query Frame = 2
Query:  377 KVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANS----IPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSI------YDR---SNIDTQNSDCRHS--GSNIIFEYSS-NFNHKHDSLTSHSS--EDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDS---DFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS--KFFEFDKCDEISSIRKMKLTANT--GTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLR 1735
            + ++ +N+FD I  +GKGG+G+VY   NK+D Q YAIK+++I   K++ ++    ++E ++LA L H NIV YHT W E  +    NSQ +      GS  N     + ++    S  +      S     +  +VS   N S  +     H I      Y +   +N+  +   C  S     ++ +     F+ + D   S SS  E+    ++C   +  + +    L+IQM+ C  +L DW+  RN       +S   DF +VR   R   Q+L+GV YIHS  V+HRD+KP N+FL      D+ ++IGDFGL+    F+  +   +   R   LT  T  GT  Y+APE   G      YD KSD+YS+G+ILLEL   F T+ E   V+  +  G         WP++   V+ L S++   RP AS +L       ES + +   N ++D+  +   L+ ENE L+++L  L+
Sbjct:  158 QTSRYLNEFDEIAQLGKGGYGKVYKVRNKLDGQFYAIKKILIK--KVSRRDCRKVLREVKVLAGLQHPNIVGYHTAWMEHVQPP--NSQCKLLKAVKGSPNNKAEQDMKEVRSVESSGSFIMFEDSAHQDPSAQSVSESVNMSVSSPDKPDHCIENAGNNYKKDSNANVAKKTKACSKSYQSQGLVCQRDKCPFSSEDDVDKSDSSCDEECPQKDLCLHRHFEI-QYHLMLHIQMKLCEKSLWDWIVERNNHFQESGNSNPYDFVDVRITTRILYQILEGVRYIHSMEVLHRDLKPRNVFL---HGADLHVRIGDFGLACRDIFQQSRDSWLQKDRSNGLTHTTGVGTCLYAAPEQLKGS----HYDFKSDMYSIGIILLELFQPFGTEMERTQVLTALRNGTVPCSYERQWPVQSNFVKLLTSSECSERPSASQML-------ESQLFHQKENLVQDLQKKVLYLEEENERLKRSLQKLQ 619          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. TrEMBL
Match: A0A5N5MM44 (Uncharacterized protein OS=Pangasianodon hypophthalmus OX=310915 GN=PHYPO_G00034410 PE=4 SV=1)

HSP 1 Score: 194.897 bits (494), Expect = 2.032e-50
Identity = 146/502 (29.08%), Postives = 240/502 (47.81%), Query Frame = 2
Query:  374 FKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHS-------SEDKGALEICKS-----------SNVNVPKMEFA------------LYIQMEFCHLNLQDWLCNRNKL--------VSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS-KFFEFDKCDEISSIRKM------KLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLRLSQ 1744
            F+ ++ +++FD I P+G+G +G+V+  +NK+D Q YA+K+++I    +   +    ++E ++L+ L H NIV YHT W E  + A          NC  +   +IP +   S           ++ S NGS++  +  NS  +     HS    +N+  +NS      S +  E  S    K    + H+        E + A E C +           S V++   ++A            LYIQM+ C  +L+DW+  RN            + Q  D ++   + +++L+GVEYIHS  ++HRD+KP NIFL  H   +  +KIGDFGL+ K    ++ +++SS   +        T   GT  Y++PE   G      YD KSD+YSVG+I +EL   F T+ E    + D+ KG    DL + WP+  + ++ L S  P  RP AS +L       + +V + L+  I       S+ + E   LR+ + +L+ SQ
Sbjct:  165 FQTSRYLSEFDEISPLGRGSYGKVFKVINKLDGQHYAVKKILIK--NVTRNDCMKVIREVKVLSSLQHLNIVGYHTAWMEHVQPA--------VKNCKPTLLATIPALETPSQTERLVEE---STSSTNGSSI--VFENSECSKDRKGHS----TNV-LKNSSNNILRSTVATEQRSQVLSKKIVHSPHNFVPCAFLGEPRSAGETCPALRWESSTLSEESYVDMSSQQWAEKHPKEVQFHLMLYIQMQLCERSLKDWIQERNSTPKPELTMSTGSFQSFDSQQASDILKKILEGVEYIHSRGIMHRDLKPRNIFLNGH---ECHVKIGDFGLACKDIVMNENEQLSSTSHVNGNAESSHTTGVGTFVYASPEQLQGS----HYDSKSDMYSVGVIAVELFQPFGTEMERVQTLSDLRKGKVPDDLYKNWPVLTEQIKLLTSMDPSLRPSASQLLQSDLFNNKDMVIHNLHQRI-------SEQEEEIIQLRRQISELQTSQ 632          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. TrEMBL
Match: A0A5G3K809 (Protein kinase domain-containing protein OS=Xenopus tropicalis OX=8364 GN=eif2ak1 PE=4 SV=1)

HSP 1 Score: 193.356 bits (490), Expect = 6.375e-50
Identity = 148/481 (30.77%), Postives = 235/481 (48.86%), Query Frame = 2
Query:  377 KVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQ----VENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSI------YDR---SNIDTQNSDCRHS--GSNIIFEYSS-NFNHKHDSLTSHSS--EDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDS---DFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS--KFFEFDKCDEISSIRKMKLTANT--GTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLR 1735
            + ++ +N+FD I  +GKGG+G+VY   NK+D Q YAIK+++I   K++ ++    ++E ++LA L H NIV YHT W E  +  N   +    V+ F N        + ++    S  +      S     +  +VS   N S  +     H I      Y +   +N+  +   C  S     ++ +     F+ + D   S SS  E+    ++C   +  + +    L+IQM+ C  +L DW+  RN       +S   DF +VR   R   Q+L+GV YIHS  V+HRD+KP N+FL      D+ ++IGDFGL+    F+  +   +   R   LT  T  GT  Y+APE   G      YD KSD+YS+G+ILLEL   F T+ E   V+  +  G         WP++   V+ L S++   RP AS +L       ES + +   N ++D+  +   L+ ENE L+++L  L+
Sbjct:  158 QTSRYLNEFDEIAQLGKGGYGKVYKVRNKLDGQFYAIKKILIK--KVSRRDCRKVLREVKVLAGLQHPNIVGYHTAWMEHVQPPNSQCKLLKAVKGFPNNKAE--QDMKEVRSVESSGSFIMFEDSAHQDPSAQSVSESVNMSVSSPDKPDHCIENAGNNYKKDSNANVAKKTKACSKSYQSQGLVCQRDKCPFSSEDDVDKSDSSCDEECPQKDLCLHRHFEI-QYHLMLHIQMKLCEKSLWDWIVERNNHFQESGNSNPYDFVDVRITTRILYQILEGVRYIHSMEVLHRDLKPRNVFL---HGADLHVRIGDFGLACRDIFQQSRDSWLQKDRSNGLTHTTGVGTCLYAAPEQLKGS----HYDFKSDMYSIGIILLELFQPFGTEMERTQVLTALRNGTVPCSYERQWPVQSNFVKLLTSSECSERPSASQML-------ESQLFHQKENLVQDLQKKVLYLEEENERLKRSLQKLQ 619          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Cavefish
Match: eif2ak1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:NCBI gene;Acc:103026818])

HSP 1 Score: 187.193 bits (474), Expect = 4.475e-51
Identity = 145/496 (29.23%), Postives = 240/496 (48.39%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCS---GSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQN-SDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFA------------LYIQMEFCHLNLQDWLCNRN-----KLV---STLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS-KFFEFDKCDEI---SSIRKMKLTANT---GTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENEL--LRKTLDDLRLSQQKQH 1756
            FQ + ++ +++F+ I  +GKG +GRV+   NK+D Q YA+K+++I    ++  +    ++E ++L+ L H NIV YHT W E  + A  N       N S   GS + +  ++  S++ ++        S         +  + S  +T   S +++ ++  + QN   C   G       +        S+ S        +E+  +S +++   ++A            LYIQM+ C  +L+DW+  RN     +L       Q  D +E   + +++L+GVEYIHS  ++HRD+KP NIFL  H      +KIGDFGL+ K    +K +++   S I K   +A+T   GT  Y++PE   G      YD KSD+YSVG+I +EL   F T+ E  H + ++ +G     L E WPL  + ++ L S  P  RP AS +L         +V   +   I         +K E E+  LRK + +L+ SQ  +H
Sbjct:  139 FQAQTSRYLSEFEEISTLGKGSYGRVFKVKNKLDGQHYAVKKILIK--NVSRDDCVKVLREVKVLSSLQHPNIVGYHTAWMEHVQPAVNNPTKPMLPNSSPALGSLSQTDGRLEESTNGSSIVFESSECSKDTKDPPTQTPQSTSVKSTPETSQAMFTKNMRNPQNFVPCVFLGKPRSVVDTCPALSWDTSVLSEDEASPNRVELNNNSYIDMDSQQWAEKHPKEVQFHLMLYIQMQLCERSLKDWIQERNCTPKAELAVAGGPYQPFDCEEAFNILKKILEGVEYIHSCGIMHRDLKPRNIFLHGHA---CHVKIGDFGLACKDIVANKQEQLHFNSHIGKNAESAHTTGVGTFVYASPEQLEGS----HYDSKSDMYSVGVIAVELFHPFGTEMERVHTLGELRRGKVPDTLAENWPLLTKQIKLLTSADPSLRPNASELLQSDLFNSRDMVIRSMQQQI---------VKQEEEIVQLRKQISELQSSQNSKH 616          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Cavefish
Match: eif2ak1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:NCBI gene;Acc:103026818])

HSP 1 Score: 186.808 bits (473), Expect = 8.192e-51
Identity = 145/496 (29.23%), Postives = 240/496 (48.39%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCS---GSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQN-SDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFA------------LYIQMEFCHLNLQDWLCNRN-----KLV---STLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLS-KFFEFDKCDEI---SSIRKMKLTANT---GTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENEL--LRKTLDDLRLSQQKQH 1756
            FQ + ++ +++F+ I  +GKG +GRV+   NK+D Q YA+K+++I    ++  +    ++E ++L+ L H NIV YHT W E  + A  N       N S   GS + +  ++  S++ ++        S         +  + S  +T   S +++ ++  + QN   C   G       +        S+ S        +E+  +S +++   ++A            LYIQM+ C  +L+DW+  RN     +L       Q  D +E   + +++L+GVEYIHS  ++HRD+KP NIFL  H      +KIGDFGL+ K    +K +++   S I K   +A+T   GT  Y++PE   G      YD KSD+YSVG+I +EL   F T+ E  H + ++ +G     L E WPL  + ++ L S  P  RP AS +L         +V   +   I         +K E E+  LRK + +L+ SQ  +H
Sbjct:  177 FQAQTSRYLSEFEEISTLGKGSYGRVFKVKNKLDGQHYAVKKILIK--NVSRDDCVKVLREVKVLSSLQHPNIVGYHTAWMEHVQPAVNNPTKPMLPNSSPALGSLSQTDGRLEESTNGSSIVFESSECSKDTKDPPTQTPQSTSVKSTPETSQAMFTKNMRNPQNFVPCVFLGKPRSVVDTCPALSWDTSVLSEDEASPNRVELNNNSYIDMDSQQWAEKHPKEVQFHLMLYIQMQLCERSLKDWIQERNCTPKAELAVAGGPYQPFDCEEAFNILKKILEGVEYIHSCGIMHRDLKPRNIFLHGHA---CHVKIGDFGLACKDIVANKQEQLHFNSHIGKNAESAHTTGVGTFVYASPEQLEGS----HYDSKSDMYSVGVIAVELFHPFGTEMERVHTLGELRRGKVPDTLAENWPLLTKQIKLLTSADPSLRPNASELLQSDLFNSRDMVIRSMQQQI---------VKQEEEIVQLRKQISELQSSQNSKH 654          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Cavefish
Match: eif2ak1 (eukaryotic translation initiation factor 2 alpha kinase 1 [Source:NCBI gene;Acc:103026818])

HSP 1 Score: 176.792 bits (447), Expect = 1.182e-47
Identity = 144/505 (28.51%), Postives = 231/505 (45.74%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTV------------WFERFEEA-NWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIY---DRSNIDTQNSDCRHSGSNIIFEYSSNF----NHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRN-----KLV---STLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKL------------TANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENEL--LRKTLDDLRLSQQKQH 1756
            FQ + ++ +++F+ I  +GKG +GRV+   NK+D Q YA+K+++I    ++  +    ++E ++L+ L H NIV YHT             W  R EE+ N +S V   + CS    +              +    STS+        ++   +  N  +    ++    RS +DT  +    +      E S N     N+ +  + S    +K   E+         +    LYIQM+ C  +L+DW+  RN     +L       Q  D +E   + +++L+GVEYIHS  ++HRD+KP NIFL  H      +KIGDFGL+       C +I + ++ +L            T   GT  Y++PE   G      YD KSD+YSVG+I +EL   F T+ E  H + ++ +G     L E WPL  + ++ L S  P  RP AS +L         +V   +   I         +K E E+  LRK + +L+ SQ  +H
Sbjct:  110 FQAQTSRYLSEFEEISTLGKGSYGRVFKVKNKLDGQHYAVKKILIK--NVSRDDCVKVLREVKVLSSLQHPNIVGYHTAWMEHVQPAVNSKWLGRLEESTNGSSIVFESSECSKDTKDP------------PTQTPQSTSVKSTPETSQAMFTKNMRNPQNFVPCVFLGKPRSVVDTCPALSWDTSVLSEDEASPNRVELNNNSYIDMDSQQWAEKHPKEV---------QFHLMLYIQMQLCERSLKDWIQERNCTPKAELAVAGGPYQPFDCEEAFNILKKILEGVEYIHSCGIMHRDLKPRNIFLHGHA---CHVKIGDFGLA-------CKDIVANKQEQLHFNSHIGKNSAHTTGVGTFVYASPEQLEGS----HYDSKSDMYSVGVIAVELFHPFGTEMERVHTLGELRRGKVPDTLAENWPLLTKQIKLLTSADPSLRPNASELLQSDLFNSRDMVIRSMQQQI---------VKQEEEIVQLRKQISELQSSQNSKH 568          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Cavefish
Match: eif2ak3 (eukaryotic translation initiation factor 2-alpha kinase 3 [Source:ZFIN;Acc:ZDB-GENE-050414-2])

HSP 1 Score: 112.079 bits (279), Expect = 2.595e-25
Identity = 81/238 (34.03%), Postives = 126/238 (52.94%), Query Frame = 2
Query:  962 LEICKSSNVNVPKM--EFALYIQMEFCHL-NLQDWLCNRNKLVSTLQDSDFKEVRCLA--RQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGL--SKFFEFDKCDEISSIRKMKL----TANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGF-----KDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKE 1627
            L  C S+    P +  +  LYIQM+ C   NL+DW+  R+      Q    +  +CLA   Q+ + V++IHS  ++HRD+KP+NIF  +    D  +K+GDFGL  +   E D  +++S++  M +    T   GT  Y +PE  +G     SY HK D+YS+G+IL EL+  F TQ E    + ++ + + F     K+ ++    E   VR ++S  P  RPEAS I  +   F+E
Sbjct:  689 LPPCPSTPSARPPLTPKVYLYIQMQLCRKENLKDWMAQRS------QPEHREHSQCLAIFLQIAEAVDFIHSKGLMHRDLKPSNIFFTL----DDVVKVGDFGLVTAMDQEEDDDEDLSTLTPMPMYARHTGQVGTKLYMSPEQMSGN----SYSHKVDIYSLGLILFELLCPFRTQMERVRTLTEV-RALQFPTAFYKNNVQ----EVSMVRCMLSRVPAERPEASEI-TEAPLFQE 906          

HSP 2 Score: 64.3142 bits (155), Expect = 2.443e-10
Identity = 34/105 (32.38%), Postives = 61/105 (58.10%), Query Frame = 2
Query:  344 VQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIA--DTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVEN 652
            V + +P ++   V++ + DF+ ++ +G+GGFG V+ A NK+D   YAIK++     +L  +E+A    ++E + LAKL H  I+RY   W E   +  W  +++ 
Sbjct:  435 VSDARPSEY---VSRYLTDFEPVQCLGRGGFGVVFEARNKVDDCNYAIKRI-----RLPNREVAREKVMREVKALAKLEHPGIIRYFNAWQESPPQG-WQEEMDQ 530          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Cavefish
Match: eif2ak2 (eukaryotic translation initiation factor 2-alpha kinase 2 [Source:ZFIN;Acc:ZDB-GENE-080422-1])

HSP 1 Score: 103.99 bits (258), Expect = 6.332e-23
Identity = 70/201 (34.83%), Postives = 102/201 (50.75%), Query Frame = 2
Query: 1013 LYIQMEFCHLN-LQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQT 1612
            LYIQME C  + LQ W+   N    + +    +E   +  Q+LK VEYIHS N+IHRD+KPANI      +K+  LK+GDFGL    E D  + +     ++ T  TGT                SYD K D++ +G+I  EL+    T  E   V +D+      +D  + + LE + +  ++S  PE RP+AS +L  T
Sbjct:  403 LYIQMELCEGDTLQFWIETENTKDPSPERR--QEAAKIMTQVLKAVEYIHSVNLIHRDLKPANIMF----TKEKVLKVGDFGLVTAAETDSDEAL-----LERTNRTGT----------------SYDKKVDIFPLGLIYFELLWNLKTGHEKAEVWKDVRSRKLPEDFSKKFMLESKLIEQMLSASPEERPDASDLLANT 576          

HSP 2 Score: 63.1586 bits (152), Expect = 5.058e-10
Identity = 33/75 (44.00%), Postives = 44/75 (58.67%), Query Frame = 2
Query:  389 LINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFE 613
             + +F+SI  +GKGGFGRV+ A   I+ + YA+K V         K  +  ++E   LAKL H NIVRYHT W E
Sbjct:  315 FLENFNSISKLGKGGFGRVFKARKVIEQKDYAVKIV---------KYTSKALREVAALAKLVHPNIVRYHTSWIE 380          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Sea Lamprey
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:ZFIN;Acc:ZDB-GENE-061013-268])

HSP 1 Score: 184.882 bits (468), Expect = 1.685e-51
Identity = 126/437 (28.83%), Postives = 206/437 (47.14%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNG-SNVSSLTNNSAFNTGSLSHSIYDRSNIDTQN-------SDCRHSGSNIIFEYS-SNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDS----DFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEI-----------SSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILN 1606
            F    ++ +++F+ +  +G+GG+G+V+   NK+D Q+YA+K+V+I  S   E      ++E ++LA L H +IV YH  W E  +     +  +   +       ++         + +   +       N  S  +S  N ++ N  S   S +  SN    N       S+ +H GSN  +E+S SN  H  +S TS +            S+     +   LYIQM+ C  +L++WL +RN L+          D K+   + +QLL+GV YIHS  ++HRD+KP NIFL      D  ++IGDFGL+       C +I           + +     T+  GT  Y+APE  +G      YD KSD+YSVG++L EL   F T+ E    ++++  G+        WP++ +++  L  + P  RP    IL 
Sbjct:   94 FHMHTSRYLSEFEEVCRLGRGGYGQVFKVRNKLDRQLYAVKKVLIKGS--GESICKKVLREVKLLAGLQHLSIVGYHGAWLELLQPPTLAAPFQPLPSAEAGCQRNVGWCPPGRFSSCHVPSLVQAHGGLNSESRDNSGHNKNSINEDSGRKSEHSGSNGKPTNLRKELYESNSKHVGSN--YEHSESNSEHSENSNTSWTP----------GSDSKDRDLHLVLYIQMQLCERSLKEWLMDRNALLQQCTGRYALVDGKQTTRMFQQLLEGVHYIHSRGIMHRDLKPRNIFL---HGPDHHVRIGDFGLA-------CQDIVYEDPRLIRAGAGVVGSAHTSGVGTTLYAAPEQLSGS----HYDFKSDMYSVGIVLFELFQPFGTEMERVKTIQELRGGVVSSPFTAQWPVQAKYIGLLTQDDPAARPNPQDILT 502          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007738.1 (pep scaffold:Pmarinus_7.0:GL476507:25231:69652:1 gene:ENSPMAG00000006975.1 transcript:ENSPMAT00000007738.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 136.732 bits (343), Expect = 1.107e-33
Identity = 134/460 (29.13%), Postives = 217/460 (47.17%), Query Frame = 2
Query:  371 QFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFE----EANWNSQVEN---------FTNCSG------SYANSIPKIXXXXXXXXXXXXXXXTSMSFN-------GSNVSSLTNNSAFNTGSLSHSIYDRSNIDT-QNSDCRHSGSNIIFEYSSNFNHK---HDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLN-LQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSK-----FFEFDKCDEISSIRKMK---------LTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMV-KFSTQSELCHVVEDIS-KGIGFKDLIETWPLEDQH--VRNLISNKPENRPEASFIL 1603
            Q ++++   +F+ +  +GKG FG V    NK+D   YA+K++ +N +    + I     E  +L++LNH NIVRY+  W ER+E    +A     V N          +  SG        AN+ P ++ S+    + SV    SMS+        G + +  + + + +       ++ RS + +  +SDC     +++FE S   N     HDS  S     K    +C+ +    P +   L+IQME+C  + L+D +   +K +    D  ++    L R++L G+ YIH   +IHRD+KP NIFL    S D  +KIGDFGL+        E  K D  ++  ++          LT   GTA Y +PEV  G  K + Y+ K DLYS+G+I  E+     +T SE   V++ +    I F +      L+ Q   +R L+++    RP A  +L
Sbjct:  606 QRQLSRYYTEFEELALLGKGAFGAVIKVKNKLDGCYYALKRIAVNPTSRQFRRIKG---EVTLLSRLNHENIVRYYNAWIERYELSPSDAKETPDVANVRGRGPAMHLSRLSGIAGLDDVEANAPPPVTASTRTVASGSVEW--SMSYEHSVSARCGPSTTGGSPSDSSDEDDEDDVVFGRSFVPSGSDSDC-----DVVFENSGEKNQSEESHDSSLSDKELSKNRAPVCQPT----PTVVHHLFIQMEYCERSTLRDTI---DKGLHMETDRMWR----LFREILDGLAYIHEQGMIHRDLKPVNIFL---DSSD-SVKIGDFGLATDHRLVATESSKIDVSTANIELPTENMDVTGGLTGQIGTALYVSPEVL-GNIKAV-YNQKVDLYSLGIIFFEMTYHPMTTASERVAVLQKLRLPTIQFPEDFHPQTLKQQAFIIRWLLNHDAGQRPTAQELL 1038          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006924.1 (pep scaffold:Pmarinus_7.0:GL476537:325917:352372:-1 gene:ENSPMAG00000006216.1 transcript:ENSPMAT00000006924.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 105.531 bits (262), Expect = 7.095e-24
Identity = 71/222 (31.98%), Postives = 108/222 (48.65%), Query Frame = 2
Query:  977 SSNVNVPKMEFALYIQMEFCHLN-LQDWLCNRNKLVSTLQDSDFKEVRCLA--RQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKL--TANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKE 1627
            +S    P     LYIQM+ C  + L++WL +R         +D     CL   +Q++  V+++H + ++HRD+KP+NIF     S D  +KIGDFGL    E ++   + +     +  T   GT  Y +PE   G+    +Y HK D++S+G+IL EL+  F TQ E    +      I     + +   E Q V  L++  P  RPEA  I  Q   F E
Sbjct:  762 ASTETGPVPRLFLYIQMQLCRKDSLKEWLNSRTNA------ADRSRAECLTIFQQIVNAVQFLHVSGLMHRDLKPSNIFF----SLDGVVKIGDFGLVTGTEHEEEGAVLTPMPGHIHHTGQVGTKLYMSPEQLAGR----AYSHKVDIFSLGLILFELLCPFGTQMERIKTLSAARNRIFPDPFVTSNVDEHQLVERLLAQDPSLRPEAEEI-AQAALFAE 968          

HSP 2 Score: 63.929 bits (154), Expect = 7.391e-11
Identity = 34/102 (33.33%), Postives = 58/102 (56.86%), Query Frame = 2
Query:  380 VNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQV-IINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVEN--FTNCSGSY 676
            V++ + DF+ ++ +G+GGFG V+ A NK+D   YAIK++ + N  +  EK     ++E + LAKL H  +VRY   W E     +W  + +    ++CS  +
Sbjct:  477 VSRYLTDFEHVQCLGRGGFGVVFEARNKVDDCHYAIKRIRLPNRIQAREK----VMREVKALAKLEHQGVVRYFNAWLE-MPPQDWQEEKDKQWISDCSSEW 573          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000555.1 (pep scaffold:Pmarinus_7.0:GL477093:35206:42652:1 gene:ENSPMAG00000000500.1 transcript:ENSPMAT00000000555.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 81.2629 bits (199), Expect = 3.080e-18
Identity = 54/166 (32.53%), Postives = 89/166 (53.61%), Query Frame = 2
Query: 1130 QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLI-ETWPLEDQHVRN-------LISNKPENRPEASFIL 1603
            Q+   V+YIHS  ++HRDIK +N+FL    +K   +K+GDFG+SK  E            M +T   GT +Y +PE+    C+ + Y  KSD++++G +L EL        E  +++    + + +K +  E  PL++ + R+       L+S  PE+RP AS +L
Sbjct:   16 QVCMAVQYIHSVKILHRDIKTSNVFL----TKAGTVKVGDFGISKAME--------GTLDMAVTC-VGTPYYLSPEM----CQDLPYSSKSDVWALGCLLYEL-CALRPAFEASNIIRLTRRSLFYKIMRGEPDPLDESYSRDVRALVALLLSRSPEDRPSASAVL 163          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Sea Lamprey
Match: stk35 (serine/threonine kinase 35 [Source:ZFIN;Acc:ZDB-GENE-061103-553])

HSP 1 Score: 76.2554 bits (186), Expect = 7.201e-16
Identity = 44/156 (28.21%), Postives = 83/156 (53.21%), Query Frame = 2
Query:  995 PKMEFALYIQMEFC-HLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFF--EFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFS 1453
            P   + L++ ME+C   ++  +L +R          D       A QL + V ++H N+++HRD+KP NI +  +  + + LK+ DFGLSK F  +        S+ +  L+   G++FY APEV+ G      +  K+D++++G+I+  ++ + +
Sbjct:    7 PGNSYYLWLVMEYCDGGDMNGYLLSRQP--------DHWLNTSFAMQLSRAVAFLHRNSIVHRDLKPDNILVAENSGRPV-LKVADFGLSKIFAGQGQGLSSGESVNRAWLSPACGSSFYMAPEVWEGH-----FTAKADIFALGIIVWAMLERIT 148          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Yeast
Match: GCN2 (Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control [Source:SGD;Acc:S000002691])

HSP 1 Score: 134.806 bits (338), Expect = 2.375e-33
Identity = 128/420 (30.48%), Postives = 207/420 (49.29%), Query Frame = 2
Query:  395 NDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGS-LSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVP----KMEFALYIQMEFCH-LNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSK-------FFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDI-SKGIGFKDLIE--TWPLEDQHVRNLISNKPENRPEASFILN 1606
            +DF+ I  +G+G FG+V  A N +D + YAIK++     +  E++++  + E  +LA LNH  +VRY+  W E       +S  EN    +   ++     S SSS    + ++  +S+  N +N     +N  F +GS     +++ S+ D +N D  H  S+     S +   K         E K    + +  N   P    K +  L+IQME+C    L D + + N  ++  +D  ++    L RQ+L+ + YIHS  +IHRD+KP NIF  I  S+++  KIGDFGL+K         + D  +   S     LT+  GTA Y A EV +G      Y+ K D+YS+G+I  E++  FST  E  ++++ + S  I F    +     +E + +R LI + P  RP A  +LN
Sbjct:  597 SDFEEIAVLGQGAFGQVVKARNALDSRYYAIKKI-----RHTEEKLSTILSEVMLLASLNHQYVVRYYAAWLEE------DSMDENVFESTDEESDL----SESSSDFEENDLLDQSSIFKNRTNHDLDNSNWDFISGSGYPDIVFENSSRDDENEDLDHDTSSTSSSESQDDTDK---------ESKSIQNVPRRRNFVKPMTAVKKKSTLFIQMEYCENRTLYDLIHSEN--LNQQRDEYWR----LFRQILEALSYIHSQGIIHRDLKPMNIF--IDESRNV--KIGDFGLAKNVHRSLDILKLDSQNLPGS--SDNLTSAIGTAMYVATEVLDGTGH---YNEKIDMYSLGIIFFEMIYPFSTGMERVNILKKLRSVSIEFPPDFDDNKMKVEKKIIRLLIDHDPNKRPGARTLLN 977          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Yeast
Match: DUN1 (Cell-cycle checkpoint S/T protein kinase; required for transient G2/M arrest after DNA damage, damage-induced transcription, and nuclear-to-cytoplasmic redistribution of Rnr2p-Rnr4p after genotoxic stress and iron deprivation; phosphorylates repair protein Rad55p, transcriptional repressor Sml1p, superoxide dismutase, and ribonucleotide reductase inhibitors Crt1p and Dif1p; functions in the Mec1p pathway to regulate dNTP pools and telomere length; postreplicative repair role [Source:SGD;Acc:S000002259])

HSP 1 Score: 78.5666 bits (192), Expect = 7.331e-16
Identity = 55/136 (40.44%), Postives = 71/136 (52.21%), Query Frame = 2
Query: 1109 EVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLII----HPSK-----------DIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANT--GTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSE 1465
            E + L +QLL G++Y+H  N+IHRDIKP NI L I    +PS+           DIQ+KI DFGL+KF             +M+ T NT  GT  Y APEV   K     Y  K DL+S G+IL   +  F   S+
Sbjct:  304 ESKALFKQLLTGLKYLHEQNIIHRDIKPENILLNITRRENPSQVQLGPWDEDEIDIQVKIADFGLAKF-----------TGEMQFT-NTLCGTPSYVAPEVLTKK----GYTSKVDLWSAGVILYVCLCGFPPFSD 423          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Yeast
Match: BCK1 (MAPKKK acting in the protein kinase C signaling pathway; the kinase C signaling pathway controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym for mitogen-activated protein (MAP) kinase kinase kinase [Source:SGD;Acc:S000003631])

HSP 1 Score: 74.3294 bits (181), Expect = 3.217e-14
Identity = 61/187 (32.62%), Postives = 91/187 (48.66%), Query Frame = 2
Query: 1112 VRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQS--ELCHVVEDISKGIGFKDLIE-TWPLEDQHVRNLISN----KPENRPEASFIL-------NQTCCFKES 1630
            ++ L  Q+LKG+ Y+HS  ++HRD+K  N+ L     +D   KI DFG+S+     K  +I S   M +    GT F+ APE+ + K     Y  K D++S+G I+LE+       S  E+   +  I K      + E T PL  Q  RN +       PE RP A+ +L       N+T  FK +
Sbjct: 1280 IKHLTTQVLKGLAYLHSKGILHRDMKADNLLL----DQDGICKISDFGISR-----KSKDIYSNSDMTM---RGTVFWMAPEMVDTKQ---GYSAKVDIWSLGCIVLEMFAGKRPWSNLEVVAAMFKIGKSKSAPPIPEDTLPLISQIGRNFLDACFEINPEKRPTANELLSHPFSEVNETFNFKST 1451          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Yeast
Match: MKK1 (MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication [Source:SGD;Acc:S000005757])

HSP 1 Score: 70.0922 bits (170), Expect = 4.051e-13
Identity = 53/153 (34.64%), Postives = 84/153 (54.90%), Query Frame = 2
Query: 1010 ALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELM-VKFSTQSE 1465
            ++YI ME+      D +  +N L    + S+ K +  +A  +L+G+ Y+H   VIHRDIKP NI L    +++ Q+K+ DFG+S        + ++S+     T  TGT+FY APE   G+     Y   SD++S+G+ +LE+   KF   SE
Sbjct:  294 SIYIAMEYMGGRSLDAIY-KNLLERGGRISE-KVLGKIAEAVLRGLSYLHEKKVIHRDIKPQNILL----NENGQVKLCDFGVS-------GEAVNSL----ATTFTGTSFYMAPERIQGQ----PYSVTSDVWSLGLTILEVANGKFPCSSE 425          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Yeast
Match: CDC5 (Polo-like kinase; controls targeting and activation of Rho1p at cell division site via Rho1p guanine nucleotide exchange factors; regulates Spc72p; also functions in adaptation to DNA damage during meiosis; regulates the shape of the nucleus and expansion of the nuclear envelope during mitosis; similar to Xenopus Plx1 and S. pombe Plo1p; human homologs PLK1, PLK3 can each complement yeast cdc5 thermosensitive mutants [Source:SGD;Acc:S000004603])

HSP 1 Score: 70.0922 bits (170), Expect = 4.732e-13
Identity = 73/260 (28.08%), Postives = 120/260 (46.15%), Query Frame = 2
Query: 1013 LYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMV-KFSTQSELCHVVEDISKGIGFKDLIETWPLEDQH---VRNLISNKPENRPEASFILNQT---CCFKESIVQNGLYN--HIKDIMSENSKLK----SENELLRKTLDDLRLSQQKQ 1753
            +YI +E C       L  R K+++        EVR    Q+   ++Y+HS  VIHRD+K  NIF       +  LKIGDFGL+     +      S RK  +    GT  Y APEV  GK    S++   D++S+G++L  L++ K   Q+   + + +  K   F       P+ D+    +R+++S  P  RP  + I++       F  SI    +    + +DI  E S +      E  LL +++   ++ +QK+
Sbjct:  154 VYILLEICPNGSLMELLKRRKVLTE------PEVRFFTTQICGAIKYMHSRRVIHRDLKLGNIFF----DSNYNLKIGDFGLAAVLANE------SERKYTI---CGTPNYIAPEVLMGKHSGHSFE--VDIWSLGVMLYALLIGKPPFQARDVNTIYERIKCRDF-SFPRDKPISDEGKILIRDILSLDPIERPSLTEIMDYVWFRGTFPPSIPSTVMSEAPNFEDIPEEQSLVNFKDCMEKSLLLESMSSDKIQRQKR 391          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Nematostella
Match: EDO46416 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQ17])

HSP 1 Score: 107.842 bits (268), Expect = 1.652e-24
Identity = 91/300 (30.33%), Postives = 143/300 (47.67%), Query Frame = 2
Query:  923 DSLTSHSSEDKGALEICKSSNVNVPK---MEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLA--RQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKD------IQLKIGDFGLSK---FFEFDKCDEISSI---------------RKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSE----NELLRKTL 1723
            +SL S S  DK      +S +  V +   +   L+IQM+ C   L DWL +RN      +  +   V  +A  RQ+++GV YIHS  ++HRD+KP NIFL     +D      +Q+KIGDFGL++       D    ++S+                K   T   GT  Y+APE    + +   Y +K+D+YS+G+IL EL   F T+ E    ++++ +G   K     WP E   V   +S + E + +      +    K  I++  L   ++   SEN KL+ E    NEL+R+ +
Sbjct:  377 NSLVSLSGNDK-----SESPSTQVARKKTLGMMLFIQMQLCTTTLCDWLQSRN-----FKGGNVDTVASMAIFRQIVEGVRYIHSQGLMHRDLKPRNIFLQSLTRRDEQTTAPLQVKIGDFGLARKDAVLYPDTSSPLASLIEPLSHHNPFLPGLSTKDAPTCGVGTCTYAAPE----QLRNTDYANKADMYSLGIILFELYWPFGTEMERVTSIKNLRQGNLPKAFSHRWPAE---VSQQLSEQLEMQSK------EIDELKNVIIEKDL--ELEAQGSENEKLQQELAERNELIRRLM 651          

HSP 2 Score: 61.6178 bits (148), Expect = 5.599e-10
Identity = 27/73 (36.99%), Postives = 46/73 (63.01%), Query Frame = 2
Query:  395 NDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFE 613
            ++F  +  +GKGGFG V    NK+D + YA+K+V++   + +       ++E ++LA+LNH N+V YH+ W E
Sbjct:  175 DEFVEMGRLGKGGFGSVLKVKNKLDGREYAVKKVLLK--ETDPGLCLKILREVKVLARLNHVNVVGYHSAWLE 245          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Nematostella
Match: EDO48785 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RIC0])

HSP 1 Score: 106.686 bits (265), Expect = 5.292e-24
Identity = 81/251 (32.27%), Postives = 122/251 (48.61%), Query Frame = 2
Query:  971 CKSSNVNVPKMEFALYIQMEFCHL-NLQDWLCNRNKLVSTLQDSDFKEVRC--LARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMK-----LTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSE 1699
            C S  ++ P     LYIQM+ C   +L+DWL N     ST +D     + C  +  Q++  VEY H+  ++HRD+KP+NIF     S D  +K+GDFGL    + D   EI             T   GT  Y +PE   GK    +Y  K D++S+G+IL EL   FST+ E   V+ ++ K I   +   +  L+ Q V  + S+ P  RP A+            I  + +Y +I  +  + +K+KSE
Sbjct:  768 CTSDILSAP---LYLYIQMQLCQRESLKDWLKNN----STRED-----IFCFNVFEQIVNAVEYFHNRGMMHRDLKPSNIFF----SLDGLVKVGDFGLVTATKQDNIHEIQETDNPGDLDGNHTGQVGTQLYMSPEQIEGK----AYSFKVDIFSLGLILFELFHPFSTEMERIKVMSNVKKRIMPAEFKTSMTLQSQLVTWMTSDLPTERPTAA-----------EIQSSDIYKNIITLSKQGTKVKSE 987          

HSP 2 Score: 65.0846 bits (157), Expect = 6.769e-11
Identity = 39/100 (39.00%), Postives = 55/100 (55.00%), Query Frame = 2
Query:  383 NQLINDFDSIKPIGKGGFGRVYSAVNKI-DCQIYAIKQV-IINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSY 676
            ++ + DF+    +GKGGFG V+ A NK+ DCQ YAIK+V + NC +   K     ++E + LAKL H  IVRY   WFE      W  +++      G Y
Sbjct:  484 SRYLTDFEHELCLGKGGFGLVFQARNKVDDCQ-YAIKRVRLPNCDEARAK----VMREVKALAKLEHSGIVRYFNAWFEA-PPVGWQEELDEKMVDDGKY 577          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Nematostella
Match: EDO35699 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKU3])

HSP 1 Score: 87.8113 bits (216), Expect = 3.387e-18
Identity = 67/205 (32.68%), Postives = 107/205 (52.20%), Query Frame = 2
Query: 1010 ALYIQMEFCHLN-LQDWLCNRNKLVSTLQDSDFKE--VRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDK-CDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMV--KFSTQSELCHVVEDISKGIGFKDLIETWPLE-DQHVRNLISNKPENRPEASFIL 1603
            +L+I+ME+C+   L D +C +N+L        F E  V     Q+   V +IH N ++HRDIK  NIFL    +K   +K+GDFG+SK  + +   D I            GT +Y +PE+  GK     Y+ KSD+++VG +L E++   +    S    +V DI KG  ++++ E +  E +  V  L+S  P++RP    +L
Sbjct:   88 SLFIEMEYCNGGTLHDKICVQNEL--------FPEEVVIWYFFQICAAVGHIHENGIVHRDIKTMNIFL----TKSGLVKLGDFGISKILDSEGMADSI-----------VGTPYYMSPEIVQGK----KYNQKSDMWAVGCVLYEVLTLKRVFDASNPLRLVSDIVKG-HYEEIDERYTEEMNSLVNKLLSQNPDDRPSVQELL 264          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Nematostella
Match: EDO49138 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH03])

HSP 1 Score: 78.9518 bits (193), Expect = 1.984e-16
Identity = 51/157 (32.48%), Postives = 85/157 (54.14%), Query Frame = 2
Query: 1130 QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFS--TQSELCHVVEDISKGIGFKDLIETW-PLEDQHVRNLISNKPENRPEA 1591
            QLL  +++IH  N++HRD+K  NIFL    +KD  +KIGDFG+++  +   CD   +          GT +Y +PE+    C+R  Y++KSD++++G +L EL  +    T   L ++V  I  G  +  +   + PL +  V  ++   P +RP A
Sbjct:  110 QLLLALKHIHGQNILHRDLKTQNIFL----TKDDVVKIGDFGIARILD-STCDHART--------TVGTPYYLSPEI----CQRQPYNNKSDIWALGCVLYELTTRTHPFTADNLTNLVVKILHG-NYPPIPRFYGPLLEDLVAVMLKINPADRPSA 248          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Nematostella
Match: EDO37567 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SFE6])

HSP 1 Score: 73.1738 bits (178), Expect = 4.271e-14
Identity = 41/110 (37.27%), Postives = 64/110 (58.18%), Query Frame = 2
Query: 1109 EVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLEL 1438
            +++ L  Q+L GV+++H++ ++HRDIKP NI +    +KD Q+KI DFGL++ ++            M LT+   T +Y APEV      + SY    D++SV  IL EL
Sbjct:  114 KIKDLTYQILNGVDFLHTHRIVHRDIKPQNILV----TKDGQVKIADFGLARVYK----------DAMALTSVVVTLWYRAPEVL----LQSSYATSVDIWSVACILAEL 205          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Medaka
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:101172779])

HSP 1 Score: 131.724 bits (330), Expect = 1.796e-31
Identity = 135/481 (28.07%), Postives = 224/481 (46.57%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXX-----------------XXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFA--LYIQMEFCHLNLQDWLCNRNKLVSTLQDS---DFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSK---------FFEFDKCDEISSIRK---MKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVK-FSTQSELCHV-----VEDISKGIGFKDLIETWPLEDQHVRN-----LISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMS 1675
             Q + ++  N+F+ ++ +GKG FG V    NK+D   YA+K++ +N +    K+      E  +L++LNH NIVRY+  W ER E  +      + ++ + S A+  PK   S                      +SSV  STS+  + S   S   +S  +       ++  S + + +SD R   S+IIF+   N +   D ++     ++ A +  +S + +  ++  A  LYIQME+C          ++ L  T+  S   D   +  L R++L G+ YIH   +IHRD+KP NIFL    S+D  +KIGDFGL+           FE +     + ++      +T   GTA Y +PEV  G  K  +Y+ K DL+S+G+IL E+  +  +T +E   V     VE IS       L E +P  +Q  +      L+ + P  RP A  +L         + ++ L+  ++  M+
Sbjct:  459 LQRQFSRYFNEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPAS---KQFRRIKGEVTLLSRLNHENIVRYYNAWIERHELPSTGIPSNSDSSEAQSPADQHPKSRASPQRMNELGLPDNVEDMAPPPALSSSVEWSTSIEKSSSAKCSGHQSSDEDEDEDDEDVFGASFLPS-DSDSR---SDIIFD---NGDESADEMSQAEKSERPATDTTESLDSDR-QLSIAHYLYIQMEYC---------EKSTLRDTIDQSLHLDQNRLWRLFREILDGLAYIHEQGMIHRDLKPVNIFL---DSQD-HVKIGDFGLATDHPANVAAGKFELEDSGSAAVLKPDPTGNMTGMVGTALYVSPEV-QGNTK-ATYNQKVDLFSLGIILFEMSYRPMTTGAERISVLGKLRVEPIS-------LPEDFPAYNQGTQRKVIEWLLKHDPALRPTAQELLKSELLPPPQMEESELHEVLQHTMA 906          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Medaka
Match: EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4 [Source:NCBI gene;Acc:101172779])

HSP 1 Score: 131.724 bits (330), Expect = 2.079e-31
Identity = 135/481 (28.07%), Postives = 224/481 (46.57%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXX-----------------XXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFA--LYIQMEFCHLNLQDWLCNRNKLVSTLQDS---DFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSK---------FFEFDKCDEISSIRK---MKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVK-FSTQSELCHV-----VEDISKGIGFKDLIETWPLEDQHVRN-----LISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMS 1675
             Q + ++  N+F+ ++ +GKG FG V    NK+D   YA+K++ +N +    K+      E  +L++LNH NIVRY+  W ER E  +      + ++ + S A+  PK   S                      +SSV  STS+  + S   S   +S  +       ++  S + + +SD R   S+IIF+   N +   D ++     ++ A +  +S + +  ++  A  LYIQME+C          ++ L  T+  S   D   +  L R++L G+ YIH   +IHRD+KP NIFL    S+D  +KIGDFGL+           FE +     + ++      +T   GTA Y +PEV  G  K  +Y+ K DL+S+G+IL E+  +  +T +E   V     VE IS       L E +P  +Q  +      L+ + P  RP A  +L         + ++ L+  ++  M+
Sbjct:  573 LQRQFSRYFNEFEELQLLGKGAFGAVIKVQNKLDGCYYAVKRIQVNPAS---KQFRRIKGEVTLLSRLNHENIVRYYNAWIERHELPSTGIPSNSDSSEAQSPADQHPKSRASPQRMNELGLPDNVEDMAPPPALSSSVEWSTSIEKSSSAKCSGHQSSDEDEDEDDEDVFGASFLPS-DSDSR---SDIIFD---NGDESADEMSQAEKSERPATDTTESLDSDR-QLSIAHYLYIQMEYC---------EKSTLRDTIDQSLHLDQNRLWRLFREILDGLAYIHEQGMIHRDLKPVNIFL---DSQD-HVKIGDFGLATDHPANVAAGKFELEDSGSAAVLKPDPTGNMTGMVGTALYVSPEV-QGNTK-ATYNQKVDLFSLGIILFEMSYRPMTTGAERISVLGKLRVEPIS-------LPEDFPAYNQGTQRKVIEWLLKHDPALRPTAQELLKSELLPPPQMEESELHEVLQHTMA 1020          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Medaka
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:NCBI gene;Acc:101174538])

HSP 1 Score: 128.642 bits (322), Expect = 7.416e-31
Identity = 80/245 (32.65%), Postives = 122/245 (49.80%), Query Frame = 2
Query: 1007 FALYIQMEFCHLNLQDWLCNRNKLVSTLQDS-------DFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEI---------SSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLK 1693
              LYIQM+ C  +L+DW+  RN        S       D +    L R +L+GVEYIH+  ++HRD+KP NIFL      D  ++IGDFGL+       C +I         SS      T+  GT  Y++PE   G      YD KSD+YS+G+I LEL   F T+ E    + D+ +G       + WP+  ++++NL S +P  R  A+ +L       + +V +GL   +++   E  +L+
Sbjct:  367 LMLYIQMQLCDRSLKDWISERNSWSKEEDISRGHYDCVDAEHTLSLLRHVLEGVEYIHAKGIMHRDLKPRNIFL---QGPDCHVRIGDFGLA-------CRDILVDGYKRSNSSGSDSIHTSGVGTFVYASPEQLKGS----HYDSKSDMYSIGVIALELFQPFGTEMERVRTLGDLREGKVPDSFHQRWPVLSKYIKNLTSKEPSVRLTATQLLQSELFSSKDMVIHGLQRRVEEQREEIMQLR 597          

HSP 2 Score: 70.4774 bits (171), Expect = 2.525e-12
Identity = 29/87 (33.33%), Postives = 57/87 (65.52%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEA 628
            F  + ++ +++F+ I  +GKG +G V+   NK+D Q YA+K+++I   K+++++    ++E ++L+ L+H N+V YHT W E  + A
Sbjct:  141 FLGQTSRYLSEFEEICRLGKGSYGNVFKVRNKLDAQFYAVKKILIK--KVSKEDCMKVLREVKVLSSLHHVNVVGYHTAWMEHVQPA 225          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Medaka
Match: eif2ak1 (eukaryotic translation initiation factor 2-alpha kinase 1 [Source:NCBI gene;Acc:101174538])

HSP 1 Score: 123.635 bits (309), Expect = 3.343e-29
Identity = 75/219 (34.25%), Postives = 110/219 (50.23%), Query Frame = 2
Query:  998 KMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDS-------DFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEI---------SSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILN 1606
            +    LYIQM+ C  +L+DW+  RN        S       D +    L R +L+GVEYIH+  ++HRD+KP NIFL      D  ++IGDFGL+       C +I         SS      T+  GT  Y++PE   G      YD KSD+YS+G+I LEL   F T+ E    + D+ +G       + WP+  ++++NL S +P  R  A+ +L 
Sbjct:  399 QFHLMLYIQMQLCDRSLKDWISERNSWSKEEDISRGHYDCVDAEHTLSLLRHVLEGVEYIHAKGIMHRDLKPRNIFL---QGPDCHVRIGDFGLA-------CRDILVDGYKRSNSSGSDSIHTSGVGTFVYASPEQLKGS----HYDSKSDMYSIGVIALELFQPFGTEMERVRTLGDLREGKVPDSFHQRWPVLSKYIKNLTSKEPSVRLTATQLLQ 603          

HSP 2 Score: 70.8626 bits (172), Expect = 2.342e-12
Identity = 29/87 (33.33%), Postives = 57/87 (65.52%), Query Frame = 2
Query:  368 FQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEA 628
            F  + ++ +++F+ I  +GKG +G V+   NK+D Q YA+K+++I   K+++++    ++E ++L+ L+H N+V YHT W E  + A
Sbjct:  176 FLGQTSRYLSEFEEICRLGKGSYGNVFKVRNKLDAQFYAVKKILIK--KVSKEDCMKVLREVKVLSSLHHVNVVGYHTAWMEHVQPA 260          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Medaka
Match: ENSORLT00000043016.1 (interferon-induced, double-stranded RNA-activated protein kinase [Source:NCBI gene;Acc:100144382])

HSP 1 Score: 116.316 bits (290), Expect = 6.824e-27
Identity = 78/212 (36.79%), Postives = 115/212 (54.25%), Query Frame = 2
Query: 1013 LYIQMEFCHL-NLQDWLCNRNKLVSTLQDSDFK-EVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQN 1642
            LYIQME C    L+DW+ ++N+    LQ    + E   +A Q++ GVEYIHSN +IHRD+KPANI   +    D ++KIGDFGL      D  DE    R     + TGT  Y APE  +G     +Y  K D++++G+I  EL+ K  T  E   + E+  +       +ET+P E   +++L+  KP++R EA+ +  +     E  VQN
Sbjct:  414 LYIQMELCRSETLKDWIKDKNR--KDLQAYQRRGESLPIALQIISGVEYIHSNGLIHRDLKPANIMFGM----DNKVKIGDFGLVTTEAVD--DEKRMAR-----SKTGTKVYMAPEQTSG-----NYGQKVDMFALGLIFFELLWKLWTGHERVQIWENARRKKFPAQFLETFPEESLIIKSLLCEKPDDRLEATAVKAEL----EKFVQN 603          

HSP 2 Score: 56.6102 bits (135), Expect = 5.184e-8
Identity = 29/72 (40.28%), Postives = 44/72 (61.11%), Query Frame = 2
Query:  398 DFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFE 613
            ++D +  IGKGGFGRVY   N++  + YA+K  I++ ++  ++E +        LAKL H NIVRY+  W E
Sbjct:  328 EYDILGRIGKGGFGRVYKVRNRLINREYAVK--IVSGTEKAKQEAS-------TLAKLQHDNIVRYYIAWME 390          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Planmine SMEST
Match: SMESG000020439.1 (SMESG000020439.1)

HSP 1 Score: 1075.08 bits (2779), Expect = 0.000e+0
Identity = 542/548 (98.91%), Postives = 545/548 (99.45%), Query Frame = 2
Query:  113 MTFNLINAPCLEMDGNFWTKATKVCNFYVIDNNVIISNTSDNAEEKTKLLVISVDIFKKCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLRLSQQKQH 1756
            MTFNLINAPCLEMDGNFWTKATKVCNFYVIDNNVIISNTSDNAEEKTKLLVISVDIFKKCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIP+ISHSSSFTNNSSVICSTSMSFNGSNVS+LTNNSAFNTGSLSHSI D SNIDT+NSDCRHS SNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLRLSQQKQH
Sbjct:    1 MTFNLINAPCLEMDGNFWTKATKVCNFYVIDNNVIISNTSDNAEEKTKLLVISVDIFKKCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPRISHSSSFTNNSSVICSTSMSFNGSNVSNLTNNSAFNTGSLSHSICDPSNIDTRNSDCRHSDSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLRLSQQKQH 548          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Planmine SMEST
Match: SMESG000020439.1 (SMESG000020439.1)

HSP 1 Score: 1048.88 bits (2711), Expect = 0.000e+0
Identity = 530/536 (98.88%), Postives = 533/536 (99.44%), Query Frame = 2
Query:  149 MDGNFWTKATKVCNFYVIDNNVIISNTSDNAEEKTKLLVISVDIFKKCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLRLSQQKQH 1756
            MDGNFWTKATKVCNFYVIDNNVIISNTSDNAEEKTKLLVISVDIFKKCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIP+ISHSSSFTNNSSVICSTSMSFNGSNVS+LTNNSAFNTGSLSHSI D SNIDT+NSDCRHS SNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLRLSQQKQH
Sbjct:    1 MDGNFWTKATKVCNFYVIDNNVIISNTSDNAEEKTKLLVISVDIFKKCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPRISHSSSFTNNSSVICSTSMSFNGSNVSNLTNNSAFNTGSLSHSICDPSNIDTRNSDCRHSDSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFALYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQTCCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLRLSQQKQH 536          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Planmine SMEST
Match: SMESG000032678.1 (SMESG000032678.1)

HSP 1 Score: 122.094 bits (305), Expect = 3.884e-29
Identity = 120/422 (28.44%), Postives = 193/422 (45.73%), Query Frame = 2
Query:  395 NDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHS-SEDKGALEICKSSNVNVPKM---EFALYIQME-FCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMK---------LTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLI-ETWPLEDQHVRNLISNKPENRPEASFILN 1606
             D++ I+ +GKG FG V+ A +++D   YA+K+V +  S     ++   VK F   A L H  IVRY+  WFE   E  W  + +        Y + +  +S SS  +   S   ST  +   S+ S  +  +  +  SL   I+DRS +         SGS  +            S+T+ S +   GAL    ++N     M      LYI M  +   +L+DWL  +    +T   +     RCL     Q++  V Y+HSN ++HRD+KP+NI           ++I DFGL+      K    + +  +          LT+N GT  Y +PE    + +   YD K D++S+G+I +ELMV  ST  E   VV   ++ + F+  + +++P E +    L++  P+ RP    I N
Sbjct:  120 TDYEHIRCLGKGAFGVVFEARHRVDQYNYAVKRVRVRYSDEARSKLLQEVKAF---AHLEHPGIVRYYQAWFELPPEG-WQERKDKEMGLCNLYDDDM-SVSSSSDQSREISNQDSTRCTATNSDWSKFSILAEGDQDSLI--IFDRSAVAP-------SGSESV------------SMTNRSVAPASGALGASGAANNTSNNMNCSHIYLYISMHLYSSRSLKDWLQEQQTSPATRPTT-----RCLLHIFAQIVDAVAYLHSNGLMHRDLKPSNILF----DNQNHIRIVDFGLATSMMRSKPPPSADVSALPSGSHGDSQSLTSNVGTELYMSPE---QQQQGTRYDQKVDVFSLGIIFIELMVPLSTGMERA-VVLTKARRMQFEPWVTDSFPREVEFACRLLNPDPDKRPTCEQIFN 502          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Planmine SMEST
Match: SMESG000032681.1 (SMESG000032681.1)

HSP 1 Score: 113.235 bits (282), Expect = 3.931e-26
Identity = 114/399 (28.57%), Postives = 183/399 (45.86%), Query Frame = 2
Query:  395 NDFDSIKPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRILAKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKIXXXXXXXXXXXXXXXTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSDCRHSGSNIIFEYSSNFNHKHDSLTSHS-SEDKGALEICKSSNVNVPKM---EFALYIQME-FCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLAR---QLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKMK---------LTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQSELCHVVEDISKGIGFKDLI-ETWPLE 1537
             D++ I+ +GKG FG V+ A +++D   YA+K+V +  S     ++   VK F   A L H  IVRY+  WFE   E  W  + +        Y + +  +S SS  +   S   ST  +   S+ S  +  +  +  SL   I+DRS +         SGS  +            S+T+ S +   GAL    ++N     M      LYI M  +   +L+DWL  +    +T   +     RCL     Q++  V Y+HSN ++HRD+KP+NI           ++I DFGL+      K    + +  +          LT+N GT  Y +PE    + +   YD K D++S+G+I +ELMV  ST  E   VV   ++ + F+  + +++P E
Sbjct:  120 TDYEHIRCLGKGAFGVVFEARHRVDQYNYAVKRVRVRYSDEARSKLLQEVKAF---AHLEHPGIVRYYQAWFELPPEG-WQERKDKEMGLCNLYDDDM-SVSSSSDQSREISNQDSTRCTATNSDWSKFSILAEGDQDSLI--IFDRSAVAP-------SGSESV------------SMTNRSVAPASGALGASGAANNTSNNMNCSHIYLYISMHLYSSRSLKDWLQEQQTSPATRPTT-----RCLLHIFAQIVDAVAYLHSNGLMHRDLKPSNILF----DNQNHIRIVDFGLATSMMRSKPPPSADVSALPSGSHGDSQSLTSNVGTELYMSPE---QQQQGTRYDQKVDVFSLGIIFIELMVPLSTGMERA-VVLTKARRMQFEPWVTDSFPRE 479          
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Planmine SMEST
Match: SMESG000021704.1 (SMESG000021704.1)

HSP 1 Score: 84.7297 bits (208), Expect = 9.941e-18
Identity = 55/140 (39.29%), Postives = 80/140 (57.14%), Query Frame = 2
Query: 1112 VRCLARQLLKGVEYIHSNNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFD-KCDEISS--IRKMKLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVK--FSTQSELCHVVEDISKGIGFKDL 1516
            +R    QLL GV YIHS +VIHRD+KPANI +    + +  LKI DFG+++    + KCD   S  +  + LT    T +Y APE+  G+   +SYD K DL+S G IL EL +K        + + +++I K +G  D+
Sbjct:  133 IRYFMYQLLCGVNYIHSASVIHRDLKPANILI----NSNCHLKIADFGMARLLTPNIKCDRKFSENVLNLNLTQYVMTRWYRAPELLMGQ---LSYDMKIDLWSCGCILAELFLKKPLFRGDNMINQLKEIIKKMGTPDV 265          
The following BLAST results are available for this feature:
BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
EIF2AK14.979e-4329.08eukaryotic translation initiation factor 2 alpha k... [more]
EIF2AK41.281e-2827.46eukaryotic translation initiation factor 2 alpha k... [more]
EIF2AK35.560e-2635.18eukaryotic translation initiation factor 2 alpha k... [more]
EIF2AK27.006e-2336.15eukaryotic translation initiation factor 2 alpha k... [more]
EIF2AK28.454e-2335.98eukaryotic translation initiation factor 2 alpha k... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pek-14.485e-2233.67Eukaryotic translation initiation factor 2-alpha k... [more]
Y38E10A.82.170e-1529.76pep chromosome:WBcel235:II:12607200:12608788:1 gen... [more]
wee-1.32.449e-1434.00Membrane-associated tyrosine- and threonine-specif... [more]
gck-13.129e-1428.57Germinal center kinase 1 [Source:UniProtKB/Swiss-... [more]
gck-13.138e-1428.57Germinal center kinase 1 [Source:UniProtKB/Swiss-... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PEK5.530e-2533.50gene:FBgn0037327 transcript:FBtr0330027[more]
PEK7.247e-2533.50gene:FBgn0037327 transcript:FBtr0078770[more]
PEK7.374e-2533.50gene:FBgn0037327 transcript:FBtr0330026[more]
Gcn23.038e-1628.63gene:FBgn0019990 transcript:FBtr0334552[more]
Gcn23.090e-1628.63gene:FBgn0019990 transcript:FBtr0334551[more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
BX072573.15.916e-3425.88eukaryotic translation initiation factor 2-alpha k... [more]
eif2ak36.650e-3425.88eukaryotic translation initiation factor 2-alpha k... [more]
eif2ak14.189e-3131.09eukaryotic translation initiation factor 2-alpha k... [more]
eif2ak14.425e-3131.84eukaryotic translation initiation factor 2-alpha k... [more]
eif2ak16.343e-3030.34eukaryotic translation initiation factor 2-alpha k... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
eif2ak14.060e-5330.77eukaryotic translation initiation factor 2 alpha k... [more]
eif2ak17.571e-4228.69eukaryotic translation initiation factor 2 alpha k... [more]
cpt1al2.557e-3827.61carnitine palmitoyltransferase 1A (liver) like [So... [more]
cpt1al6.621e-3727.40carnitine palmitoyltransferase 1A (liver) like [So... [more]
eif2ak45.670e-3230.55eukaryotic translation initiation factor 2 alpha k... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Eif2ak13.283e-4529.62eukaryotic translation initiation factor 2 alpha k... [more]
Eif2ak48.562e-3030.38eukaryotic translation initiation factor 2 alpha k... [more]
Eif2ak49.343e-3030.38eukaryotic translation initiation factor 2 alpha k... [more]
Eif2ak49.521e-3030.19eukaryotic translation initiation factor 2 alpha k... [more]
Eif2ak49.831e-3030.38eukaryotic translation initiation factor 2 alpha k... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q4R8E0|E2AK1_MACFA1.647e-4429.04Eukaryotic translation initiation factor 2-alpha k... [more]
sp|Q9Z2R9|E2AK1_MOUSE2.297e-4429.62Eukaryotic translation initiation factor 2-alpha k... [more]
sp|Q9BQI3|E2AK1_HUMAN2.391e-4229.08Eukaryotic translation initiation factor 2-alpha k... [more]
sp|Q63185|E2AK1_RAT2.442e-4129.02Eukaryotic translation initiation factor 2-alpha k... [more]
sp|Q9HGN1|GCN2_SCHPO5.287e-3629.46eIF-2-alpha kinase GCN2 OS=Schizosaccharomyces pom... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1L8EXZ42.938e-5131.07Protein kinase domain-containing protein OS=Xenopu... [more]
A0A099ZQU17.592e-5130.99Eukaryotic translation initiation factor 2-alpha k... [more]
Q28EX61.670e-5031.19Eukaryotic translation initiation factor 2 alpha k... [more]
A0A5N5MM442.032e-5029.08Uncharacterized protein OS=Pangasianodon hypophtha... [more]
A0A5G3K8096.375e-5030.77Protein kinase domain-containing protein OS=Xenopu... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
eif2ak14.475e-5129.23eukaryotic translation initiation factor 2 alpha k... [more]
eif2ak18.192e-5129.23eukaryotic translation initiation factor 2 alpha k... [more]
eif2ak11.182e-4728.51eukaryotic translation initiation factor 2 alpha k... [more]
eif2ak32.595e-2534.03eukaryotic translation initiation factor 2-alpha k... [more]
eif2ak26.332e-2334.83eukaryotic translation initiation factor 2-alpha k... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
eif2ak11.685e-5128.83eukaryotic translation initiation factor 2-alpha k... [more]
ENSPMAT00000007738.11.107e-3329.13pep scaffold:Pmarinus_7.0:GL476507:25231:69652:1 g... [more]
ENSPMAT00000006924.17.095e-2431.98pep scaffold:Pmarinus_7.0:GL476537:325917:352372:-... [more]
ENSPMAT00000000555.13.080e-1832.53pep scaffold:Pmarinus_7.0:GL477093:35206:42652:1 g... [more]
stk357.201e-1628.21serine/threonine kinase 35 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
GCN22.375e-3330.48Protein kinase; phosphorylates the alpha-subunit o... [more]
DUN17.331e-1640.44Cell-cycle checkpoint S/T protein kinase; required... [more]
BCK13.217e-1432.62MAPKKK acting in the protein kinase C signaling pa... [more]
MKK14.051e-1334.64MAPKK involved in the protein kinase C signaling p... [more]
CDC54.732e-1328.08Polo-like kinase; controls targeting and activatio... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO464161.652e-2430.33Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO487855.292e-2432.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO356993.387e-1832.68Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO491381.984e-1632.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO375674.271e-1437.27Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
EIF2AK41.796e-3128.07eukaryotic translation initiation factor 2 alpha k... [more]
EIF2AK42.079e-3128.07eukaryotic translation initiation factor 2 alpha k... [more]
eif2ak17.416e-3132.65eukaryotic translation initiation factor 2-alpha k... [more]
eif2ak13.343e-2934.25eukaryotic translation initiation factor 2-alpha k... [more]
ENSORLT00000043016.16.824e-2736.79interferon-induced, double-stranded RNA-activated ... [more]
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BLAST of Eukaryotic translation initiation factor 2-alpha kinase 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000020439.10.000e+098.91SMESG000020439.1[more]
SMESG000020439.10.000e+098.88SMESG000020439.1[more]
SMESG000032678.13.884e-2928.44SMESG000032678.1[more]
SMESG000032681.13.931e-2628.57SMESG000032681.1[more]
SMESG000021704.19.941e-1839.29SMESG000021704.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003352 ID=SMED30003352|Name=Eukaryotic translation initiation factor 2-alpha kinase 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1829bp
GAAATTTCAAACTTCCATCATAATTTATAAATGTTTTAAGAATCTTGTTA
TACCATTAATAAAAGTCTCATAAAAAATCCGATACGAATATATTTTTTTT
CAAAATTGTTTAATGACATTTAATTTAATTAATGCGCCATGTTTGGAAAT
GGATGGCAATTTTTGGACAAAGGCTACAAAAGTTTGTAATTTTTACGTGA
TTGATAATAATGTAATCATTTCTAACACATCTGATAATGCAGAAGAAAAA
ACAAAACTTTTAGTTATTAGTGTAGACATTTTTAAGAAATGTTTTGGCGA
TAATTTTATTTCTCCAAATTTCATACAGAATTCGATTTCTTTTGTCCAAA
ACTTTAAGCCAATCAAATTTCAGTTCAAAGTGAATCAATTGATAAATGAT
TTTGATTCCATCAAACCTATTGGAAAAGGCGGGTTTGGTAGAGTATATTC
AGCTGTAAATAAAATTGACTGTCAAATTTATGCCATTAAACAAGTGATTA
TAAATTGCTCAAAATTGAATGAAAAAGAAATCGCAGATACGGTGAAAGAG
TTTCGAATATTAGCGAAATTAAATCATGGAAATATTGTGAGATATCACAC
AGTCTGGTTCGAAAGATTTGAAGAAGCAAATTGGAATTCTCAAGTTGAGA
ACTTTACCAATTGCAGCGGTTCCTATGCTAATAGCATTCCAAAAATCTCG
CACTCTTCATCTTTTACCAATAATTCTAGTGTAATTTGTTCGACTTCAAT
GTCTTTCAATGGATCGAATGTGTCAAGCCTGACCAATAACTCCGCTTTCA
ATACTGGATCTTTATCTCATTCAATATATGATCGGTCTAATATTGATACA
CAAAACAGTGATTGTCGTCATTCTGGTTCAAACATTATTTTTGAGTATTC
TTCGAACTTTAATCATAAACACGATTCTTTGACAAGTCACTCTTCAGAAG
ATAAAGGAGCTCTCGAAATATGCAAGTCTTCCAATGTTAATGTGCCGAAA
ATGGAATTTGCACTTTATATACAAATGGAATTCTGTCATTTAAACTTGCA
AGATTGGCTATGTAATCGAAATAAACTAGTCAGCACTTTACAAGATTCTG
ACTTTAAAGAAGTCAGATGTCTTGCTCGTCAGTTGTTAAAGGGCGTTGAA
TACATTCATTCAAACAATGTCATACATCGAGACATCAAGCCTGCTAATAT
ATTTCTGATTATTCATCCTAGCAAGGATATTCAATTGAAAATTGGTGATT
TTGGTCTTTCTAAATTCTTTGAATTCGATAAGTGCGATGAGATAAGTTCT
ATTAGAAAAATGAAATTGACAGCAAACACCGGGACGGCTTTTTACAGTGC
GCCAGAAGTATTTAATGGAAAATGTAAAAGAATTTCGTATGATCACAAGT
CTGATTTGTATTCCGTTGGTATGATTCTTCTTGAGCTAATGGTCAAATTC
TCAACTCAATCTGAATTGTGTCATGTAGTCGAAGATATATCTAAAGGAAT
CGGATTCAAAGATCTGATAGAAACATGGCCATTGGAAGATCAACATGTCC
GAAATTTAATTTCAAATAAACCTGAAAATAGACCTGAAGCTAGTTTTATA
TTAAATCAAACTTGCTGCTTTAAAGAATCAATTGTCCAGAATGGATTATA
TAATCACATCAAAGATATTATGTCAGAAAATTCAAAATTGAAAAGTGAGA
ACGAGTTACTGCGTAAAACACTTGATGACCTGCGCTTATCTCAGCAAAAG
CAACATTGAAAACAGTTTTGTGAATAAATTTATGAATTTATATTTATTGG
GTTTTATTTTTCCTGAATTTGAAAGATTA
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protein sequence of SMED30003352-orf-1

>SMED30003352-orf-1 ID=SMED30003352-orf-1|Name=SMED30003352-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=549bp
MTFNLINAPCLEMDGNFWTKATKVCNFYVIDNNVIISNTSDNAEEKTKLL
VISVDIFKKCFGDNFISPNFIQNSISFVQNFKPIKFQFKVNQLINDFDSI
KPIGKGGFGRVYSAVNKIDCQIYAIKQVIINCSKLNEKEIADTVKEFRIL
AKLNHGNIVRYHTVWFERFEEANWNSQVENFTNCSGSYANSIPKISHSSS
FTNNSSVICSTSMSFNGSNVSSLTNNSAFNTGSLSHSIYDRSNIDTQNSD
CRHSGSNIIFEYSSNFNHKHDSLTSHSSEDKGALEICKSSNVNVPKMEFA
LYIQMEFCHLNLQDWLCNRNKLVSTLQDSDFKEVRCLARQLLKGVEYIHS
NNVIHRDIKPANIFLIIHPSKDIQLKIGDFGLSKFFEFDKCDEISSIRKM
KLTANTGTAFYSAPEVFNGKCKRISYDHKSDLYSVGMILLELMVKFSTQS
ELCHVVEDISKGIGFKDLIETWPLEDQHVRNLISNKPENRPEASFILNQT
CCFKESIVQNGLYNHIKDIMSENSKLKSENELLRKTLDDLRLSQQKQH*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR000719Prot_kinase_dom
IPR017441Protein_kinase_ATP_BS
IPR008271Ser/Thr_kinase_AS
IPR011009Kinase-like_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0004672protein kinase activity
GO:0005524ATP binding
GO:0003743translation initiation factor activity
GO:0016301kinase activity
Vocabulary: biological process
TermDefinition
GO:0006468protein phosphorylation
GO:0006413translational initiation
GO:0016310phosphorylation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 516..543
NoneNo IPR availableGENE3DG3DSA:1.10.510.10coord: 309..509
e-value: 1.8E-40
score: 140.4
NoneNo IPR availableGENE3DG3DSA:3.30.200.20coord: 87..200
e-value: 1.5E-23
score: 84.9
NoneNo IPR availablePANTHERPTHR11042EUKARYOTIC TRANSLATION INITIATION FACTOR 2-ALPHA KINASE EIF2-ALPHA KINASE -RELATEDcoord: 85..521
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 97..502
e-value: 5.6E-28
score: 108.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 98..166
e-value: 2.7E-11
score: 43.3
coord: 300..449
e-value: 1.8E-29
score: 102.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 97..508
score: 34.139
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 103..126
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 353..365
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 91..498