slc12a-4

Overview


Name	slc12a-4 (AKN21478.1)
Smed ID	SMED30003346
Length (bp)	3681

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of slc12a-4 (SMED30003346) t-SNE clustered cells

Violin plots show distribution of expression levels for slc12a-4 (SMED30003346) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of slc12a-4 (SMED30003346) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for slc12a-4 (SMED30003346) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30003346

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 14

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
head region	SMED30003346	SMESG000019076.1	SmedASXL_017003	SmedAsxl_ww_GCZZ01	PMID:27034770 Currie et al., 2016
protonephridia	SMED30003346	SMESG000019076.1	dd_Smed_v4_4389_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
nervous system	SMED30003346	SMESG000019076.1	dd_Smed_v4_4389_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
cephalic ganglia	SMED30003346	SMESG000019076.1	dd_Smed_v4_4389_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
neoblast	SMED30003346	SMESG000019076.1	dd_Smed_v4_4389_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
parenchymal cell	SMED30003346	SMESG000019076.1	dd_Smed_v4_4389_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
muscle cell	SMED30003346	SMESG000019076.1	dd_Smed_v4_4389_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
non-ciliated neuron	SMED30003346	SMESG000019076.1	dd_Smed_v4_4389_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30003346	SMESG000019076.1 SMESG000052056.1 SMESG000025956.1	Contig42190	uc_Smed_v2	PMID:29674431 Fincher et al., 2018
Smed sexual biotype	SMED30003346	SMESG000019076.1 SMESG000052056.1 SMESG000025956.1	Contig42190	newmark_ests	PMID:29674431 Fincher et al., 2018
head region	SMED30003346	SMESG000019076.1	dd_Smed_v6_4389_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017
photoreceptor neuron	SMED30003346	SMESG000019076.1	SMED30003346	smed_20140614	PMID:22884275 Lapan et al., 2012
distal tubule	SMED30003346	SMESG000019076.1 SMESG000052056.1 SMESG000025956.1	Smed-slc12a-4	smed_ncbi_20200123	PMID:21828097 Rink et al., 2011
collecting duct	SMED30003346	SMESG000019076.1 SMESG000052056.1 SMESG000025956.1	Smed-slc12a-4	smed_ncbi_20200123	PMID:21828097 Rink et al., 2011

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Homology

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BLAST of slc12a-4 vs. Ensembl Human
Match: SLC12A5 (solute carrier family 12 member 5 [Source:HGNC Symbol;Acc:HGNC:13818])

HSP 1 Score: 933.324 bits (2411), Expect = 0.000e+0
Identity = 501/1167 (42.93%), Postives = 709/1167 (60.75%), Query Frame = 3

Query:  132 EKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGD---SKFDLYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIEL--DENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEP-------HLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            E   H V + +    E++  +  P        S D E   +       L+EEE  T P ++++ + LA YT          E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G++G    F +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   +   +   M NN RVYG  +L  M + VF+G+K+V+KF+ + L CV+ SIL +Y G+   A    +  +C+LG+R L+R    D  A   +   + +   L+ L+C   ++ LN    +YF  NNVTE   IPG    L+K+  + +Y T KG +    G           +D       SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP G+I A ATTS VY+S+V+ F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAI++D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRF+YYHWT S LG+ LC+ALMF+ SWYYALVA+ +AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   +    +  A+E++ R +  +++KG  ++V++SN+ + +  L Q+ GLG L+HN V+V +P  W   + H  W+ F+  +R   A  LA+LV K +  FP    +               G++D+W IVHDGG+L+LL  LL   + W KC++R+F VA   DN+I +KK++  F+Y LRI A+VEVVE+ ++DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+            +I   +P          N  +  N P    EET  D +  P+E  + ++   +    S++               ++       T D + A  +  P        SP +  G+K  FS     +N      RRMH+AVRLNE+I  +S +A L+L+N+PGP +N + D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   22 ESRRHSVADPRHLPGEDVKGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHG-FDVCAKLAWEGNETVTTRLWGLFC--SSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLT-KGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSE------------GSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESR---------GSIRRKNP---------ANTRLRLNVP----EETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEK--VHLTWTKDKSVAEKNKGP--------SPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS 1139

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BLAST of slc12a-4 vs. Ensembl Human
Match: SLC12A5 (solute carrier family 12 member 5 [Source:HGNC Symbol;Acc:HGNC:13818])

HSP 1 Score: 929.472 bits (2401), Expect = 0.000e+0
Identity = 499/1148 (43.47%), Postives = 705/1148 (61.41%), Query Frame = 3

Query:  189 KEATPLTDEEIEMSDDEEPAGD---SKFDLYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIEL--DENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEP-------HLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            KE++P  +     S D E   +       L+EEE  T P ++++ + LA YT          E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G++G    F +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   +   +   M NN RVYG  +L  M + VF+G+K+V+KF+ + L CV+ SIL +Y G+   A    +  +C+LG+R L+R    D  A   +   + +   L+ L+C   ++ LN    +YF  NNVTE   IPG    L+K+  + +Y T KG +    G           +D       SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP G+I A ATTS VY+S+V+ F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAI++D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRF+YYHWT S LG+ LC+ALMF+ SWYYALVA+ +AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   +    +  A+E++ R +  +++KG  ++V++SN+ + +  L Q+ GLG L+HN V+V +P  W   + H  W+ F+  +R   A  LA+LV K +  FP    +               G++D+W IVHDGG+L+LL  LL   + W KC++R+F VA   DN+I +KK++  F+Y LRI A+VEVVE+ ++DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+            +I   +P          N  +  N P    EET  D +  P+E  + ++   +    S++               ++       T D + A  +  P        SP +  G+K  FS     +N      RRMH+AVRLNE+I  +S +A L+L+N+PGP +N + D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   23 KESSPFIN-----STDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHG-FDVCAKLAWEGNETVTTRLWGLFC--SSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLT-KGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSE------------GSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESR---------GSIRRKNP---------ANTRLRLNVP----EETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEK--VHLTWTKDKSVAEKNKGP--------SPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS 1116

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BLAST of slc12a-4 vs. Ensembl Human
Match: SLC12A6 (solute carrier family 12 member 6 [Source:HGNC Symbol;Acc:HGNC:10914])

HSP 1 Score: 927.546 bits (2396), Expect = 0.000e+0
Identity = 522/1196 (43.65%), Postives = 721/1196 (60.28%), Query Frame = 3

Query:   87 SEVINSENDLSSVA----AEKAAH--YVVESKVENSENITKEATPLTDE------EIEMSDDEEPAGDSKFD----LYEEEFHTRPPMANIFNKLAQYT----GGIEPTSGET---KKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIE-LDENI-MFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVAN-PSRSLRVCMLGDRLLNRSVV----LDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKG---QSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKES-MNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            SE+  +   L++VA    +++ +H   V+E   +NS  IT E + L D+         +++     GD  FD    L+EEE  TRP ++++ N++A YT    G  E    E     K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G +G    F IV +CCC TMLT +SMSAIATNG VPAGGSYFMISR+LGPEFGG VG+ FY GTT AAAMYI+GAIE+ L YIVP+A++F   + L E+  M NN RVYG   L +M+  VFIG+++V+KF+++ LACV+ SIL +Y G   ++       VCMLG+R L+   +      KE NN    +K     L+  +C N ++  N    +YF  NNVT    IPG    ++ +  + NY   KG +  +P  +     G       + DI  SFT+L+GIFFPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTSFVYLS V+ F A +E  VLRDKFG +V   L+V  L+WP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+DN+IPFL VF  +   GEP  A  LT  I+ LG+LIAS+D +AP+++MFFLMCY FVNLA AL   L+ P+WRPRF+YYHW  S +G+ +C+ALMF+SSWYYA+VA+ +AG IYKYIEY+GA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ LDE    + HP+L+ F SQLKAG+GLTIVG+VI G   +   +++ A++T+   +  +++KG  ++VVA+ + E I  L Q+ GLG ++HN V++ +P  W   +    WK F+ T+RV  A+ LA+LV K I  FP        +NV         G +D+W IVHDGG+L+LL  LL + + W KC +R+F VA  +DN+I +KK++  F+Y LRI A+VEVVE+  +DISAY ++RT+ +  R ++L  M L   +  RE Q + D++                      S L   ++   E++ TE  +E V     H    K+  M S   K KS    Q L+                            +M P++ N                        R+  T  +++      E+I ++S  A L+L+N+PGP +N   D NYM+++E LT+GL RVL VRG G EVIT ++
Sbjct:    2 SEMSGATTSLATVALDPPSDRTSHPQDVIEDLSQNS--ITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSK-----LWGFFC-NSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLP-KGEIIEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDL-HVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQLVVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFP--------SNVEQFSE----GNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRN----------------------SMLRLTSIGSDEDEETETYQEKV-----HMTWTKDKYMASRGQKAKSMEGFQDLL----------------------------NMRPDQSNV-----------------------RRMHTAVKLN------EVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS 1091

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Query:   87 SEVINSENDLSSVA----AEKAAH--YVVESKVENSENITKEATPLTDE------EIEMSDDEEPAGDSKFD----LYEEEFHTRPPMANIFNKLAQYT----GGIEPTSGET---KKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIE-LDENI-MFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVAN-PSRSLRVCMLGDRLLNRSVV----LDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKG---QSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKES-MNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            SE+  +   L++VA    +++ +H   V+E   +NS  IT E + L D+         +++     GD  FD    L+EEE  TRP ++++ N++A YT    G  E    E     K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G +G    F IV +CCC TMLT +SMSAIATNG VPAGGSYFMISR+LGPEFGG VG+ FY GTT AAAMYI+GAIE+ L YIVP+A++F   + L E+  M NN RVYG   L +M+  VFIG+++V+KF+++ LACV+ SIL +Y G   ++       VCMLG+R L+   +      KE NN    +K     L+  +C N ++  N    +YF  NNVT    IPG    ++ +  + NY   KG +  +P  +     G       + DI  SFT+L+GIFFPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTSFVYLS V+ F A +E  VLRDKFG +V   L+V  L+WP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+DN+IPFL VF  +   GEP  A  LT  I+ LG+LIAS+D +AP+++MFFLMCY FVNLA AL   L+ P+WRPRF+YYHW  S +G+ +C+ALMF+SSWYYA+VA+ +AG IYKYIEY+GA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ LDE    + HP+L+ F SQLKAG+GLTIVG+VI G   +   +++ A++T+   +  +++KG  ++VVA+ + E I  L Q+ GLG ++HN V++ +P  W   +    WK F+ T+RV  A+ LA+LV K I  FP        +NV         G +D+W IVHDGG+L+LL  LL + + W KC +R+F VA  +DN+I +KK++  F+Y LRI A+VEVVE+  +DISAY ++RT+ +  R ++L  M L   +  RE Q + D++                      S L   ++   E++ TE  +E V     H    K+  M S   K KS    Q L+                            +M P++ N                        R+  T  +++      E+I ++S  A L+L+N+PGP +N   D NYM+++E LT+GL RVL VRG G EVIT ++
Sbjct:    2 SEMSGATTSLATVALDPPSDRTSHPQDVIEDLSQNS--ITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSK-----LWGFFC-NSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLP-KGEIIEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDL-HVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQLVVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFP--------SNVEQFSE----GNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRN----------------------SMLRLTSIGSDEDEETETYQEKV-----HMTWTKDKYMASRGQKAKSMEGFQDLL----------------------------NMRPDQSNV-----------------------RRMHTAVKLN------EVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS 1091

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BLAST of slc12a-4 vs. Ensembl Human
Match: SLC12A6 (solute carrier family 12 member 6 [Source:HGNC Symbol;Acc:HGNC:10914])

HSP 1 Score: 926.776 bits (2394), Expect = 0.000e+0
Identity = 524/1196 (43.81%), Postives = 718/1196 (60.03%), Query Frame = 3

Query:   87 SEVINSENDLSSVA----AEKAAH--YVVESKVENSENITKEATPLTDE------EIEMSDDEEPAGDSKFD----LYEEEFHTRPPMANIFNKLAQYT----GGIEPTSGET---KKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIE-LDENI-MFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVAN-PSRSLRVCMLGDRLLNRSVV----LDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKG---QSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKES-MNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            SE+  +   L++VA    +++ +H   V+E   +NS  IT E + L D+         +++     GD  FD    L+EEE  TRP ++++ N++A YT    G  E    E     K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G +G    F IV +CCC TMLT +SMSAIATNG VPAGGSYFMISR+LGPEFGG VG+ FY GTT AAAMYI+GAIE+ L YIVP+A++F   + L E+  M NN RVYG   L +M+  VFIG+++V+KF+++ LACV+ SIL +Y G   ++       VCMLG+R L+   +      KE NN    +K     L+  +C N ++  N    +YF  NNVT    IPG    ++ +  + NY   KG +  +P  +     G       + DI  SFT+L+GIFFPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTSFVYLS V+ F A +E  VLRDKFG +V   L+V  L+WP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+DN+IPFL VF  +   GEP  A  LT  I+ LG+LIAS+D +AP+++MFFLMCY FVNLA AL   L+ P+WRPRF+YYHW  S +G+ +C+ALMF+SSWYYA+VA+ +AG IYKYIEY+GA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ LDE    + HP+L+ F SQLKAG+GLTIVG+VI G   +   +++ A++T+   +  +++KG  ++VVA+ + E I  L Q+ GLG ++HN V++ +P  W   +    WK F+ T+RV  A+ LA+LV K I  FP        +NV         G +D+W IVHDGG+L+LL  LL + + W KC +R+F VA  +DN+I +KK++  F+Y LRI A+VEVVE+  +DISAY ++RT+ +  R ++L  M L   +  RE Q + D++                      S L   ++   E++ TE  +E V     H    K+  M S   K KS    Q L+         N R         D +N R      K+NE                                        +I ++S  A L+L+N+PGP +N   D NYM+++E LT+GL RVL VRG G EVIT ++
Sbjct:   61 SEMSGATTSLATVALDPPSDRTSHPQDVIEDLSQNS--ITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFHSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEINNMTVPSK-----LWGFFC-NSSQFFNATCDEYFVHNNVTSIQGIPGLASGIITENLWSNYLP-KGEIIEKPSAKSSDVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDL-HVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFCQLVVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFP--------SNVEQFS----EGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRN----------------------SMLRLTSIGSDEDEETETYQEKV-----HMTWTKDKYMASRGQKAKSMEGFQDLL---------NMRP--------DQSNVRRMHTAVKLNE----------------------------------------VIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS 1150

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BLAST of slc12a-4 vs. Ensembl Celegans
Match: kcc-2 (K+/Cl-Cotransporter [Source:UniProtKB/TrEMBL;Acc:V6CJH3])

HSP 1 Score: 788.489 bits (2035), Expect = 0.000e+0
Identity = 460/1147 (40.10%), Postives = 664/1147 (57.89%), Query Frame = 3

Query:  180 NITKEATPLTD-----EEIEMSDDEEPAGDSKFDLYEEEFHTR-PPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVA-NPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCR-NGTKDLNDEACKYFKENNVTEFPAI------PGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSV--SDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            N   EA  L D     E I + +   P       LYE+++ T+   +  +  KL+ Y    E TS E  +  + ++ K+GTI+GV+ PCLQNIFGVLFF+RL WIIG +G    F +V  C   T LT +S+SAIATNG VP+GG Y+MISR+LGPE GG VGILFY GTT+AA+MYI GAIE++L YI P+A LF  I       ++N RV G +LL I+   V  G+KFV++ +   +  V+  IL   +G+FV  + S SL+ CM+GDR ++ S   +K       CT D    L  L+C  NGT D   +  K  K    +  PAI       G    +  D  +  Y      L  +   +  L++G  PS   ++   +F IL+G+FFPS TGIMAGSNRSG+L +   SIPLG++AA   +SF+YL  V+ F A+V    +RDK+G S    LI+++++WP   VIL G F+ST GAG+QSLTGAPRLLQAIA D+V+PFL+ F+    RGEP+RA  LT  I   G+LIA I++I  LIT FFLMCY  VN A AL   L+ P WRP F+Y+HW+ S++G ILC+A+MF+S+W++AL AI +  G+YKYIEY GA KEWGD  RG  +SAA+ A+L L DKP H++NWRPQ+L+L    E+        ++ F SQLKAG+GLT+V   +EGE  D    +   +E L   ++  +IKG  +++V SNVIE I  L QTSGLG +RHN V++S+P +W  ++  +   +F++ +R   A+  A++VPK  ++FP   +K             ++G +D+W +VHDGGLL+LL  LL + + W    +R+F +A  +DN + +K ++ +F+Y LRI+A V V+E+  +DIS Y ++RTM +  R + L+  NL   D  ++ Q     H         ++ IN+ +P +                P ++  E V+DE+    E+ +S N       S+  E   +   D    ++ + V     TL+ +                                  + ++     +MH+AV+LNE++R +SS+A L+ VNLPGP   +S  Y YM ++E LT+GL RVL VRGTG EV+T ++
Sbjct:   13 NPNNEAIRLLDIDEGAERIPLCEQHSP-----LALYEQDYDTQGQKIGTMLRKLSVYNA-TEATSVEADEKPKAAAAKMGTIMGVFLPCLQNIFGVLFFIRLAWIIGTAGVFQAFFVVLTCVSVTFLTSISLSAIATNGVVPSGGPYYMISRNLGPELGGAVGILFYLGTTIAASMYITGAIEILLLYIYPQAKLFDDI-------YHNFRVLGTVLLLILGLIVMAGVKFVNRCALPLVIVVILCILSAILGVFVRYDGSDSLKFCMVGDRPVDLSTYFEKTQVQP-NCTAD---GLQDLFCSTNGTCDHYYDRMKNIKVWKSSGMPAIREERAIKGIASGVFFDNLWPKYLRAGEVLSKDRKDKGDLNRGGHPSYIYAESVTNFMILVGVFFPSATGIMAGSNRSGNLRDAAKSIPLGTLAAQNFSSFIYLLGVVLFGASVSEMFIRDKYGRSAMGKLIISEISWPFPQVILFGCFMSTAGAGMQSLTGAPRLLQAIAADDVLPFLKPFRKMDSRGEPIRAILLTLAICECGILIAVIENITALITQFFLMCYLGVNAACALQSLLKSPGWRPGFRYFHWSLSMIGAILCVAVMFISAWHFALFAIIIGAGVYKYIEYAGAEKEWGDGLRGLGLSAARFALLNLDDKPQHSRNWRPQLLVLAPDVESANTNG---ILSFVSQLKAGKGLTLVAHCMEGEYADNYLKAQAVQEKLKAVVKKNKIKGFCDVLVTSNVIEGISCLVQTSGLGGMRHNTVVLSWPNDWKAEQEWVVANKFVSAIRAISAAKCAIMVPKYAEKFPANGTK-------------VSGFIDVWWVVHDGGLLMLLPFLLRQHKTWKNTTVRLFAIAQMEDNNVQMKTDLEKFLYHLRIDAAVNVIEMTDSDISDYTYERTMKMEERNQYLK--NLNKSDRDKDIQ----NHLEIVTRERKLSRINEEAPAVV---------------PEQRNLE-VVDEEQ---EDGKSENG------SAKIEHKGVRFSDD---EDSKEVKVGNGTLERDREE------------------------------RQRKRRYNVHKMHTAVKLNELMRQKSSDAQLVFVNLPGPPDADSDSY-YMDFIEALTEGLDRVLLVRGTGAEVVTIYS 1061

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BLAST of slc12a-4 vs. Ensembl Celegans
Match: kcc-2 (K+/Cl-Cotransporter [Source:UniProtKB/TrEMBL;Acc:V6CJH3])

HSP 1 Score: 788.104 bits (2034), Expect = 0.000e+0
Identity = 453/1117 (40.56%), Postives = 655/1117 (58.64%), Query Frame = 3

Query:  255 SKFDLYEEEFHTR-PPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVA-NPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCR-NGTKDLNDEACKYFKENNVTEFPAI------PGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSV--SDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            S   LYE+++ T+   +  +  KL+ Y    E TS E  +  + ++ K+GTI+GV+ PCLQNIFGVLFF+RL WIIG +G    F +V  C   T LT +S+SAIATNG VP+GG Y+MISR+LGPE GG VGILFY GTT+AA+MYI GAIE++L YI P+A LF  I       ++N RV G +LL I+   V  G+KFV++ +   +  V+  IL   +G+FV  + S SL+ CM+GDR ++ S   +K       CT D    L  L+C  NGT D   +  K  K    +  PAI       G    +  D  +  Y      L  +   +  L++G  PS   ++   +F IL+G+FFPS TGIMAGSNRSG+L +   SIPLG++AA   +SF+YL  V+ F A+V    +RDK+G S    LI+++++WP   VIL G F+ST GAG+QSLTGAPRLLQAIA D+V+PFL+ F+    RGEP+RA  LT  I   G+LIA I++I  LIT FFLMCY  VN A AL   L+ P WRP F+Y+HW+ S++G ILC+A+MF+S+W++AL AI +  G+YKYIEY GA KEWGD  RG  +SAA+ A+L L DKP H++NWRPQ+L+L    E+        ++ F SQLKAG+GLT+V   +EGE  D    +   +E L   ++  +IKG  +++V SNVIE I  L QTSGLG +RHN V++S+P +W  ++  +   +F++ +R   A+  A++VPK  ++FP   +K             ++G +D+W +VHDGGLL+LL  LL + + W    +R+F +A  +DN + +K ++ +F+Y LRI+A V V+E+  +DIS Y ++RTM +  R + L+  NL   D  ++ Q     H         ++ IN+ +P +                P ++  E V+DE+    E+ +S N       S+  E   +   D    ++ + V     TL+ +                                  + ++     +MH+AV+LNE++R +SS+A L+ VNLPGP   +S  Y YM ++E LT+GL RVL VRGTG EV+T ++
Sbjct:  106 SPLALYEQDYDTQGQKIGTMLRKLSVYNA-TEATSVEADEKPKAAAAKMGTIMGVFLPCLQNIFGVLFFIRLAWIIGTAGVFQAFFVVLTCVSVTFLTSISLSAIATNGVVPSGGPYYMISRNLGPELGGAVGILFYLGTTIAASMYITGAIEILLLYIYPQAKLFDDI-------YHNFRVLGTVLLLILGLIVMAGVKFVNRCALPLVIVVILCILSAILGVFVRYDGSDSLKFCMVGDRPVDLSTYFEKTQVQP-NCTAD---GLQDLFCSTNGTCDHYYDRMKNIKVWKSSGMPAIREERAIKGIASGVFFDNLWPKYLRAGEVLSKDRKDKGDLNRGGHPSYIYAESVTNFMILVGVFFPSATGIMAGSNRSGNLRDAAKSIPLGTLAAQNFSSFIYLLGVVLFGASVSEMFIRDKYGRSAMGKLIISEISWPFPQVILFGCFMSTAGAGMQSLTGAPRLLQAIAADDVLPFLKPFRKMDSRGEPIRAILLTLAICECGILIAVIENITALITQFFLMCYLGVNAACALQSLLKSPGWRPGFRYFHWSLSMIGAILCVAVMFISAWHFALFAIIIGAGVYKYIEYAGAEKEWGDGLRGLGLSAARFALLNLDDKPQHSRNWRPQLLVLAPDVESANTNG---ILSFVSQLKAGKGLTLVAHCMEGEYADNYLKAQAVQEKLKAVVKKNKIKGFCDVLVTSNVIEGISCLVQTSGLGGMRHNTVVLSWPNDWKAEQEWVVANKFVSAIRAISAAKCAIMVPKYAEKFPANGTK-------------VSGFIDVWWVVHDGGLLMLLPFLLRQHKTWKNTTVRLFAIAQMEDNNVQMKTDLEKFLYHLRIDAAVNVIEMTDSDISDYTYERTMKMEERNQYLK--NLNKSDRDKDIQ----NHLEIVTRERKLSRINEEAPAV---------------VPEQRNLE-VVDEEQ---EDGKSENG------SAKIEHKGVRFSDD---EDSKEVKVGNGTLERDREE------------------------------RQRKRRYNVHKMHTAVKLNELMRQKSSDAQLVFVNLPGPPDADSDSY-YMDFIEALTEGLDRVLLVRGTGAEVVTIYS 1129

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BLAST of slc12a-4 vs. Ensembl Celegans
Match: kcc-2 (K+/Cl-Cotransporter [Source:UniProtKB/TrEMBL;Acc:V6CJH3])

HSP 1 Score: 788.104 bits (2034), Expect = 0.000e+0
Identity = 458/1143 (40.07%), Postives = 662/1143 (57.92%), Query Frame = 3

Query:  177 ENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTR-PPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVA-NPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCR-NGTKDLNDEACKYFKENNVTEFPAI------PGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSV--SDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            EN   E  PL ++             S   LYE+++ T+   +  +  KL+ Y    E TS E  +  + ++ K+GTI+GV+ PCLQNIFGVLFF+RL WIIG +G    F +V  C   T LT +S+SAIATNG VP+GG Y+MISR+LGPE GG VGILFY GTT+AA+MYI GAIE++L YI P+A LF  I       ++N RV G +LL I+   V  G+KFV++ +   +  V+  IL   +G+FV  + S SL+ CM+GDR ++ S   +K       CT D    L  L+C  NGT D   +  K  K    +  PAI       G    +  D  +  Y      L  +   +  L++G  PS   ++   +F IL+G+FFPS TGIMAGSNRSG+L +   SIPLG++AA   +SF+YL  V+ F A+V    +RDK+G S    LI+++++WP   VIL G F+ST GAG+QSLTGAPRLLQAIA D+V+PFL+ F+    RGEP+RA  LT  I   G+LIA I++I  LIT FFLMCY  VN A AL   L+ P WRP F+Y+HW+ S++G ILC+A+MF+S+W++AL AI +  G+YKYIEY GA KEWGD  RG  +SAA+ A+L L DKP H++NWRPQ+L+L    E+        ++ F SQLKAG+GLT+V   +EGE  D    +   +E L   ++  +IKG  +++V SNVIE I  L QTSGLG +RHN V++S+P +W  ++  +   +F++ +R   A+  A++VPK  ++FP   +K             ++G +D+W +VHDGGLL+LL  LL + + W    +R+F +A  +DN + +K ++ +F+Y LRI+A V V+E+  +DIS Y ++RTM +  R + L+  NL   D  ++ Q     H         ++ IN+ +P +                P ++  E V+DE+    E+ +S N       S+  E   +   D    ++ + V     TL+ +                                  + ++     +MH+AV+LNE++R +SS+A L+ VNLPGP   +S  Y YM ++E LT+GL RVL VRGTG EV+T ++
Sbjct:   42 ENEGAERIPLCEQH------------SPLALYEQDYDTQGQKIGTMLRKLSVYNA-TEATSVEADEKPKAAAAKMGTIMGVFLPCLQNIFGVLFFIRLAWIIGTAGVFQAFFVVLTCVSVTFLTSISLSAIATNGVVPSGGPYYMISRNLGPELGGAVGILFYLGTTIAASMYITGAIEILLLYIYPQAKLFDDI-------YHNFRVLGTVLLLILGLIVMAGVKFVNRCALPLVIVVILCILSAILGVFVRYDGSDSLKFCMVGDRPVDLSTYFEKTQVQP-NCTAD---GLQDLFCSTNGTCDHYYDRMKNIKVWKSSGMPAIREERAIKGIASGVFFDNLWPKYLRAGEVLSKDRKDKGDLNRGGHPSYIYAESVTNFMILVGVFFPSATGIMAGSNRSGNLRDAAKSIPLGTLAAQNFSSFIYLLGVVLFGASVSEMFIRDKYGRSAMGKLIISEISWPFPQVILFGCFMSTAGAGMQSLTGAPRLLQAIAADDVLPFLKPFRKMDSRGEPIRAILLTLAICECGILIAVIENITALITQFFLMCYLGVNAACALQSLLKSPGWRPGFRYFHWSLSMIGAILCVAVMFISAWHFALFAIIIGAGVYKYIEYAGAEKEWGDGLRGLGLSAARFALLNLDDKPQHSRNWRPQLLVLAPDVESANTNG---ILSFVSQLKAGKGLTLVAHCMEGEYADNYLKAQAVQEKLKAVVKKNKIKGFCDVLVTSNVIEGISCLVQTSGLGGMRHNTVVLSWPNDWKAEQEWVVANKFVSAIRAISAAKCAIMVPKYAEKFPANGTK-------------VSGFIDVWWVVHDGGLLMLLPFLLRQHKTWKNTTVRLFAIAQMEDNNVQMKTDLEKFLYHLRIDAAVNVIEMTDSDISDYTYERTMKMEERNQYLK--NLNKSDRDKDIQ----NHLEIVTRERKLSRINEEAPAVV---------------PEQRNLE-VVDEEQ---EDGKSENG------SAKIEHKGVRFSDD---EDSKEVKVGNGTLERDREE------------------------------RQRKRRYNVHKMHTAVKLNELMRQKSSDAQLVFVNLPGPPDADSDSY-YMDFIEALTEGLDRVLLVRGTGAEVVTIYS 1079

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BLAST of slc12a-4 vs. Ensembl Celegans
Match: kcc-2 (K+/Cl-Cotransporter [Source:UniProtKB/TrEMBL;Acc:V6CJH3])

HSP 1 Score: 787.719 bits (2033), Expect = 0.000e+0
Identity = 453/1117 (40.56%), Postives = 655/1117 (58.64%), Query Frame = 3

Query:  255 SKFDLYEEEFHTR-PPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVA-NPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCR-NGTKDLNDEACKYFKENNVTEFPAI------PGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSV--SDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            S   LYE+++ T+   +  +  KL+ Y    E TS E  +  + ++ K+GTI+GV+ PCLQNIFGVLFF+RL WIIG +G    F +V  C   T LT +S+SAIATNG VP+GG Y+MISR+LGPE GG VGILFY GTT+AA+MYI GAIE++L YI P+A LF  I       ++N RV G +LL I+   V  G+KFV++ +   +  V+  IL   +G+FV  + S SL+ CM+GDR ++ S   +K       CT D    L  L+C  NGT D   +  K  K    +  PAI       G    +  D  +  Y      L  +   +  L++G  PS   ++   +F IL+G+FFPS TGIMAGSNRSG+L +   SIPLG++AA   +SF+YL  V+ F A+V    +RDK+G S    LI+++++WP   VIL G F+ST GAG+QSLTGAPRLLQAIA D+V+PFL+ F+    RGEP+RA  LT  I   G+LIA I++I  LIT FFLMCY  VN A AL   L+ P WRP F+Y+HW+ S++G ILC+A+MF+S+W++AL AI +  G+YKYIEY GA KEWGD  RG  +SAA+ A+L L DKP H++NWRPQ+L+L    E+        ++ F SQLKAG+GLT+V   +EGE  D    +   +E L   ++  +IKG  +++V SNVIE I  L QTSGLG +RHN V++S+P +W  ++  +   +F++ +R   A+  A++VPK  ++FP   +K             ++G +D+W +VHDGGLL+LL  LL + + W    +R+F +A  +DN + +K ++ +F+Y LRI+A V V+E+  +DIS Y ++RTM +  R + L+  NL   D  ++ Q     H         ++ IN+ +P +                P ++  E V+DE+    E+ +S N       S+  E   +   D    ++ + V     TL+ +                                  + ++     +MH+AV+LNE++R +SS+A L+ VNLPGP   +S  Y YM ++E LT+GL RVL VRGTG EV+T ++
Sbjct:   79 SPLALYEQDYDTQGQKIGTMLRKLSVYNA-TEATSVEADEKPKAAAAKMGTIMGVFLPCLQNIFGVLFFIRLAWIIGTAGVFQAFFVVLTCVSVTFLTSISLSAIATNGVVPSGGPYYMISRNLGPELGGAVGILFYLGTTIAASMYITGAIEILLLYIYPQAKLFDDI-------YHNFRVLGTVLLLILGLIVMAGVKFVNRCALPLVIVVILCILSAILGVFVRYDGSDSLKFCMVGDRPVDLSTYFEKTQVQP-NCTAD---GLQDLFCSTNGTCDHYYDRMKNIKVWKSSGMPAIREERAIKGIASGVFFDNLWPKYLRAGEVLSKDRKDKGDLNRGGHPSYIYAESVTNFMILVGVFFPSATGIMAGSNRSGNLRDAAKSIPLGTLAAQNFSSFIYLLGVVLFGASVSEMFIRDKYGRSAMGKLIISEISWPFPQVILFGCFMSTAGAGMQSLTGAPRLLQAIAADDVLPFLKPFRKMDSRGEPIRAILLTLAICECGILIAVIENITALITQFFLMCYLGVNAACALQSLLKSPGWRPGFRYFHWSLSMIGAILCVAVMFISAWHFALFAIIIGAGVYKYIEYAGAEKEWGDGLRGLGLSAARFALLNLDDKPQHSRNWRPQLLVLAPDVESANTNG---ILSFVSQLKAGKGLTLVAHCMEGEYADNYLKAQAVQEKLKAVVKKNKIKGFCDVLVTSNVIEGISCLVQTSGLGGMRHNTVVLSWPNDWKAEQEWVVANKFVSAIRAISAAKCAIMVPKYAEKFPANGTK-------------VSGFIDVWWVVHDGGLLMLLPFLLRQHKTWKNTTVRLFAIAQMEDNNVQMKTDLEKFLYHLRIDAAVNVIEMTDSDISDYTYERTMKMEERNQYLK--NLNKSDRDKDIQ----NHLEIVTRERKLSRINEEAPAV---------------VPEQRNLE-VVDEEQ---EDGKSENG------SAKIEHKGVRFSDD---EDSKEVKVGNGTLERDREE------------------------------RQRKRRYNVHKMHTAVKLNELMRQKSSDAQLVFVNLPGPPDADSDSY-YMDFIEALTEGLDRVLLVRGTGAEVVTIYS 1102

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BLAST of slc12a-4 vs. Ensembl Celegans
Match: kcc-2 (K+/Cl-Cotransporter [Source:UniProtKB/TrEMBL;Acc:V6CJH3])

HSP 1 Score: 780.4 bits (2014), Expect = 0.000e+0
Identity = 448/1098 (40.80%), Postives = 645/1098 (58.74%), Query Frame = 3

Query:  309 IFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVA-NPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCR-NGTKDLNDEACKYFKENNVTEFPAI------PGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSV--SDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            +  KL+ Y    E TS E  +  + ++ K+GTI+GV+ PCLQNIFGVLFF+RL WIIG +G    F +V  C   T LT +S+SAIATNG VP+GG Y+MISR+LGPE GG VGILFY GTT+AA+MYI GAIE++L YI P+A LF  I       ++N RV G +LL I+   V  G+KFV++ +   +  V+  IL   +G+FV  + S SL+ CM+GDR ++ S   +K       CT D    L  L+C  NGT D   +  K  K    +  PAI       G    +  D  +  Y      L  +   +  L++G  PS   ++   +F IL+G+FFPS TGIMAGSNRSG+L +   SIPLG++AA   +SF+YL  V+ F A+V    +RDK+G S    LI+++++WP   VIL G F+ST GAG+QSLTGAPRLLQAIA D+V+PFL+ F+    RGEP+RA  LT  I   G+LIA I++I  LIT FFLMCY  VN A AL   L+ P WRP F+Y+HW+ S++G ILC+A+MF+S+W++AL AI +  G+YKYIEY GA KEWGD  RG  +SAA+ A+L L DKP H++NWRPQ+L+L    E+        ++ F SQLKAG+GLT+V   +EGE  D    +   +E L   ++  +IKG  +++V SNVIE I  L QTSGLG +RHN V++S+P +W  ++  +   +F++ +R   A+  A++VPK  ++FP   +K             ++G +D+W +VHDGGLL+LL  LL + + W    +R+F +A  +DN + +K ++ +F+Y LRI+A V V+E+  +DIS Y ++RTM +  R + L+  NL   D  ++ Q     H         ++ IN+ +P +                P ++  E V+DE+    E+ +S N       S+  E   +   D    ++ + V     TL+ +                                  + ++     +MH+AV+LNE++R +SS+A L+ VNLPGP   +S  Y YM ++E LT+GL RVL VRGTG EV+T ++
Sbjct:    1 MLRKLSVYNA-TEATSVEADEKPKAAAAKMGTIMGVFLPCLQNIFGVLFFIRLAWIIGTAGVFQAFFVVLTCVSVTFLTSISLSAIATNGVVPSGGPYYMISRNLGPELGGAVGILFYLGTTIAASMYITGAIEILLLYIYPQAKLFDDI-------YHNFRVLGTVLLLILGLIVMAGVKFVNRCALPLVIVVILCILSAILGVFVRYDGSDSLKFCMVGDRPVDLSTYFEKTQVQP-NCTAD---GLQDLFCSTNGTCDHYYDRMKNIKVWKSSGMPAIREERAIKGIASGVFFDNLWPKYLRAGEVLSKDRKDKGDLNRGGHPSYIYAESVTNFMILVGVFFPSATGIMAGSNRSGNLRDAAKSIPLGTLAAQNFSSFIYLLGVVLFGASVSEMFIRDKYGRSAMGKLIISEISWPFPQVILFGCFMSTAGAGMQSLTGAPRLLQAIAADDVLPFLKPFRKMDSRGEPIRAILLTLAICECGILIAVIENITALITQFFLMCYLGVNAACALQSLLKSPGWRPGFRYFHWSLSMIGAILCVAVMFISAWHFALFAIIIGAGVYKYIEYAGAEKEWGDGLRGLGLSAARFALLNLDDKPQHSRNWRPQLLVLAPDVESANTNG---ILSFVSQLKAGKGLTLVAHCMEGEYADNYLKAQAVQEKLKAVVKKNKIKGFCDVLVTSNVIEGISCLVQTSGLGGMRHNTVVLSWPNDWKAEQEWVVANKFVSAIRAISAAKCAIMVPKYAEKFPANGTK-------------VSGFIDVWWVVHDGGLLMLLPFLLRQHKTWKNTTVRLFAIAQMEDNNVQMKTDLEKFLYHLRIDAAVNVIEMTDSDISDYTYERTMKMEERNQYLK--NLNKSDRDKDIQ----NHLEIVTRERKLSRINEEAPAVV---------------PEQRNLE-VVDEEQ---EDGKSENG------SAKIEHKGVRFSDD---EDSKEVKVGNGTLERDREE------------------------------RQRKRRYNVHKMHTAVKLNELMRQKSSDAQLVFVNLPGPPDADSDSY-YMDFIEALTEGLDRVLLVRGTGAEVVTIYS 1005

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BLAST of slc12a-4 vs. Ensembl Fly
Match: kcc (gene:FBgn0261794 transcript:FBtr0072139)

HSP 1 Score: 933.324 bits (2411), Expect = 0.000e+0
Identity = 504/1150 (43.83%), Postives = 723/1150 (62.87%), Query Frame = 3

Query:  150 VVESKVENSENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSL--YGEPGKEPHLDKGQSPS--VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTI-NVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPE-KINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSK--NESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            V E+K E+S +I +             ++++ + D    LY+++  TRP ++   + +A Y   I P + +        S ++GT++GV+ PC+QNIFGV+ F+RL W++G +G   GF+IV  CCC TMLT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+LFY GTT+AAAMYI+GA+E++L Y+ P AS+FG    D + M+NN RVYG +LL  M   VF+G+KFV+KF+T++LACV+ SI+ VY+GIF   + +  L +C+LG RLL + + L+        CTK+ +  L  +YC +G  +      +Y+  NNVT+   I G    +  D  F ++   KG    YG+   +     G+S +  ++DI  SFT+LIGIFFPSVTGIMAGSNRSGDLA+ Q SIP+G+I A  TTS VYLS+V+FF+ TV++ +LRDKFG S+G  L+VA +AWPN+WVILIGSFLST+GAGLQSLTGAPRLLQAIA+D +IPFL  F  ++KRGEP RA  LT  I   G+L+ ++D +APL++MFFLMCYGFVNLA A+   L+ P+WRPRFK+YHW+ SL+GL LCI++M ++SWY+AL+A+ +A  IYKYIEYRGA KEWGD  RG  ++AA+ ++L+L + P HTKNWRPQIL+L  L++      + K+  F +QLKAG+GLTI  +VI+G+     + ++ AK TL + + D+++KG  +++VA  + E +  + QT GLG ++ N V++ +P  W  +  +  WK F+ T+R   A  +A++VPKGI+ +P    K             + G +D+W IVHDGGLL+LL  LL + R W  C+LR+F VA  +DN+I +KK++  F+Y LRI A VEVVE++ +DISAY ++RT+ +  R ++L  + L   +  +  Q I+D H+ A            T+  + F+  PTI     H++Q  EK     LD   + D                        TP++T  +++ +            A GD     K +EF                            RRMH+A++LNE+I  +S +A L+++NLPGP +      + NYM+++E LT+GL +VL VRG G+EVIT ++
Sbjct:   24 VHETKNEDSGDIHRA-----------EENQKSSIDPNLYLYDDDLETRPHISTFISSIANYENTI-PAATDPDAKPAAPSARMGTLIGVFLPCIQNIFGVILFIRLTWVVGTAGAVCGFLIVLTCCCVTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGMLFYTGTTLAAAMYIVGAVEIVLTYMAPWASIFGDFTKDADAMYNNFRVYGTLLLIFMGLIVFLGVKFVNKFATVALACVILSIIAVYVGIFDNIHGNEKLYMCVLGKRLL-KDIPLEN-------CTKE-DSFLRDIYCPDGKCE------EYYLANNVTKVKGIKGLASGVFYDNIFPSFLE-KGQFISYGKSAIDIENTSGESYNQIMADITTSFTLLIGIFFPSVTGIMAGSNRSGDLADAQKSIPIGTICAILTTSTVYLSSVMFFAGTVDNLLLRDKFGQSIGGKLVVANIAWPNQWVILIGSFLSTLGAGLQSLTGAPRLLQAIARDEIIPFLAPFAKSSKRGEPTRALLLTIVICQCGILLGNVDLLAPLLSMFFLMCYGFVNLACAVQTLLRTPNWRPRFKFYHWSLSLIGLTLCISVMIMTSWYFALIAMGMAIIIYKYIEYRGAEKEWGDGIRGMALTAARYSLLRLEEGPPHTKNWRPQILVLSKLNDNLLP-KYRKIFSFATQLKAGKGLTICVSVIKGDHTKITNKAVDAKATLRKYMTDEKVKGFCDVLVAQQIGEGLSSVIQTIGLGGMKPNTVIIGWPYSWRQEGRN-SWKTFIQTVRTVAACHMALMVPKGINFYPESNHK-------------IGGNIDIWWIVHDGGLLMLLPFLLKQHRTWRNCKLRIFTVAQIEDNSIQMKKDLKTFLYHLRIEADVEVVEMNNSDISAYTYERTLMMEQRNQMLRALGLNKKENSKVVQTIVDHHYDAT----------KTASKVRFAD-PTIEETQHHDSQNDEKRNSIDLDGPENAD------------------------TPETTSNKDEST----------EKADGDFKSSVKPDEFNV--------------------------RRMHTAIKLNEVIVEKSQDAQLVIMNLPGPPREVRAERERNYMEFLEVLTEGLEKVLMVRGGGREVITIYS 1059

[Close]

BLAST of slc12a-4 vs. Ensembl Fly
Match: kcc (gene:FBgn0261794 transcript:FBtr0343301)

HSP 1 Score: 932.939 bits (2410), Expect = 0.000e+0
Identity = 506/1154 (43.85%), Postives = 728/1154 (63.08%), Query Frame = 3

Query:  150 VVESKVENSENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSL--YGEPGKEPHLDKGQSPS--VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKT-----EETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPE-KINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSK--NESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            V E+K E+S +I +             ++++ + D    LY+++  TRP ++   + +A Y   I P + +        S ++GT++GV+ PC+QNIFGV+ F+RL W++G +G   GF+IV  CCC TMLT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+LFY GTT+AAAMYI+GA+E++L Y+ P AS+FG    D + M+NN RVYG +LL  M   VF+G+KFV+KF+T++LACV+ SI+ VY+GIF   + +  L +C+LG RLL + + L+        CTK+ +  L  +YC +G  +      +Y+  NNVT+   I G    +  D  F ++   KG    YG+   +     G+S +  ++DI  SFT+LIGIFFPSVTGIMAGSNRSGDLA+ Q SIP+G+I A  TTS VYLS+V+FF+ TV++ +LRDKFG S+G  L+VA +AWPN+WVILIGSFLST+GAGLQSLTGAPRLLQAIA+D +IPFL  F  ++KRGEP RA  LT  I   G+L+ ++D +APL++MFFLMCYGFVNLA A+   L+ P+WRPRFK+YHW+ SL+GL LCI++M ++SWY+AL+A+ +A  IYKYIEYRGA KEWGD  RG  ++AA+ ++L+L + P HTKNWRPQIL+L  L++      + K+  F +QLKAG+GLTI  +VI+G+     + ++ AK TL + + D+++KG  +++VA  + E +  + QT GLG ++ N V++ +P  W  +  +  WK F+ T+R   A  +A++VPKGI+ +P    K             + G +D+W IVHDGGLL+LL  LL + R W  C+LR+F VA  +DN+I +KK++  F+Y LRI A VEVVE++ +DISAY ++RT+ +  R ++L  + L   +  +  Q I+D                +T  D   S + TI VD H +     +     + T+ +  +H  +N E  NS I      N +     TP++T  +++ +            A GD     K +EF                            RRMH+A++LNE+I  +S +A L+++NLPGP +      + NYM+++E LT+GL +VL VRG G+EVIT ++
Sbjct:   24 VHETKNEDSGDIHRA-----------EENQKSSIDPNLYLYDDDLETRPHISTFISSIANYENTI-PAATDPDAKPAAPSARMGTLIGVFLPCIQNIFGVILFIRLTWVVGTAGAVCGFLIVLTCCCVTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGMLFYTGTTLAAAMYIVGAVEIVLTYMAPWASIFGDFTKDADAMYNNFRVYGTLLLIFMGLIVFLGVKFVNKFATVALACVILSIIAVYVGIFDNIHGNEKLYMCVLGKRLL-KDIPLEN-------CTKE-DSFLRDIYCPDGKCE------EYYLANNVTKVKGIKGLASGVFYDNIFPSFLE-KGQFISYGKSAIDIENTSGESYNQIMADITTSFTLLIGIFFPSVTGIMAGSNRSGDLADAQKSIPIGTICAILTTSTVYLSSVMFFAGTVDNLLLRDKFGQSIGGKLVVANIAWPNQWVILIGSFLSTLGAGLQSLTGAPRLLQAIARDEIIPFLAPFAKSSKRGEPTRALLLTIVICQCGILLGNVDLLAPLLSMFFLMCYGFVNLACAVQTLLRTPNWRPRFKFYHWSLSLIGLTLCISVMIMTSWYFALIAMGMAIIIYKYIEYRGAEKEWGDGIRGMALTAARYSLLRLEEGPPHTKNWRPQILVLSKLNDNLLP-KYRKIFSFATQLKAGKGLTICVSVIKGDHTKITNKAVDAKATLRKYMTDEKVKGFCDVLVAQQIGEGLSSVIQTIGLGGMKPNTVIIGWPYSWRQEGRN-SWKTFIQTVRTVAACHMALMVPKGINFYPESNHK-------------IGGNIDIWWIVHDGGLLMLLPFLLKQHRTWRNCKLRIFTVAQIEDNSIQMKKDLKTFLYHLRIEADVEVVEMNNSDISAYTYERTLMMEQRNQMLRALGLNKKENSKVVQTIMD--------------YKETPSDNKMSLVQTI-VDHHYDATKTASKVRFADPTIEETQHHDSQNDEKRNS-IDLDGPENAD-----TPETTSNKDEST----------EKADGDFKSSVKPDEFNV--------------------------RRMHTAIKLNEVIVEKSQDAQLVIMNLPGPPREVRAERERNYMEFLEVLTEGLEKVLMVRGGGREVITIYS 1077

[Close]

BLAST of slc12a-4 vs. Ensembl Fly
Match: kcc (gene:FBgn0261794 transcript:FBtr0072137)

HSP 1 Score: 931.784 bits (2407), Expect = 0.000e+0
Identity = 498/1123 (44.35%), Postives = 713/1123 (63.49%), Query Frame = 3

Query:  231 DDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSL--YGEPGKEPHLDKGQSPS--VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTI-NVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPE-KINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSK--NESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            ++++ + D    LY+++  TRP ++   + +A Y   I P + +        S ++GT++GV+ PC+QNIFGV+ F+RL W++G +G   GF+IV  CCC TMLT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+LFY GTT+AAAMYI+GA+E++L Y+ P AS+FG    D + M+NN RVYG +LL  M   VF+G+KFV+KF+T++LACV+ SI+ VY+GIF   + +  L +C+LG RLL + + L+        CTK+ +  L  +YC +G  +      +Y+  NNVT+   I G    +  D  F ++   KG    YG+   +     G+S +  ++DI  SFT+LIGIFFPSVTGIMAGSNRSGDLA+ Q SIP+G+I A  TTS VYLS+V+FF+ TV++ +LRDKFG S+G  L+VA +AWPN+WVILIGSFLST+GAGLQSLTGAPRLLQAIA+D +IPFL  F  ++KRGEP RA  LT  I   G+L+ ++D +APL++MFFLMCYGFVNLA A+   L+ P+WRPRFK+YHW+ SL+GL LCI++M ++SWY+AL+A+ +A  IYKYIEYRGA KEWGD  RG  ++AA+ ++L+L + P HTKNWRPQIL+L  L++      + K+  F +QLKAG+GLTI  +VI+G+     + ++ AK TL + + D+++KG  +++VA  + E +  + QT GLG ++ N V++ +P  W  +  +  WK F+ T+R   A  +A++VPKGI+ +P    K             + G +D+W IVHDGGLL+LL  LL + R W  C+LR+F VA  +DN+I +KK++  F+Y LRI A VEVVE++ +DISAY ++RT+ +  R ++L  + L   +  +  Q I+D H+ A            T+  + F+  PTI     H++Q  EK     LD   + D                        TP++T  +++ +            A GD     K +EF                            RRMH+A++LNE+I  +S +A L+++NLPGP +      + NYM+++E LT+GL +VL VRG G+EVIT ++
Sbjct:   55 ENQKSSIDPNLYLYDDDLETRPHISTFISSIANYENTI-PAATDPDAKPAAPSARMGTLIGVFLPCIQNIFGVILFIRLTWVVGTAGAVCGFLIVLTCCCVTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGMLFYTGTTLAAAMYIVGAVEIVLTYMAPWASIFGDFTKDADAMYNNFRVYGTLLLIFMGLIVFLGVKFVNKFATVALACVILSIIAVYVGIFDNIHGNEKLYMCVLGKRLL-KDIPLEN-------CTKE-DSFLRDIYCPDGKCE------EYYLANNVTKVKGIKGLASGVFYDNIFPSFLE-KGQFISYGKSAIDIENTSGESYNQIMADITTSFTLLIGIFFPSVTGIMAGSNRSGDLADAQKSIPIGTICAILTTSTVYLSSVMFFAGTVDNLLLRDKFGQSIGGKLVVANIAWPNQWVILIGSFLSTLGAGLQSLTGAPRLLQAIARDEIIPFLAPFAKSSKRGEPTRALLLTIVICQCGILLGNVDLLAPLLSMFFLMCYGFVNLACAVQTLLRTPNWRPRFKFYHWSLSLIGLTLCISVMIMTSWYFALIAMGMAIIIYKYIEYRGAEKEWGDGIRGMALTAARYSLLRLEEGPPHTKNWRPQILVLSKLNDNLLP-KYRKIFSFATQLKAGKGLTICVSVIKGDHTKITNKAVDAKATLRKYMTDEKVKGFCDVLVAQQIGEGLSSVIQTIGLGGMKPNTVIIGWPYSWRQEGRN-SWKTFIQTVRTVAACHMALMVPKGINFYPESNHK-------------IGGNIDIWWIVHDGGLLMLLPFLLKQHRTWRNCKLRIFTVAQIEDNSIQMKKDLKTFLYHLRIEADVEVVEMNNSDISAYTYERTLMMEQRNQMLRALGLNKKENSKVVQTIVDHHYDA----------TKTASKVRFAD-PTIEETQHHDSQNDEKRNSIDLDGPENAD------------------------TPETTSNKDEST----------EKADGDFKSSVKPDEFNV--------------------------RRMHTAIKLNEVIVEKSQDAQLVIMNLPGPPREVRAERERNYMEFLEVLTEGLEKVLMVRGGGREVITIYS 1074

[Close]

BLAST of slc12a-4 vs. Ensembl Fly
Match: kcc (gene:FBgn0261794 transcript:FBtr0343300)

HSP 1 Score: 931.784 bits (2407), Expect = 0.000e+0
Identity = 498/1123 (44.35%), Postives = 713/1123 (63.49%), Query Frame = 3

Query:  231 DDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSL--YGEPGKEPHLDKGQSPS--VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTI-NVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPE-KINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSK--NESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            ++++ + D    LY+++  TRP ++   + +A Y   I P + +        S ++GT++GV+ PC+QNIFGV+ F+RL W++G +G   GF+IV  CCC TMLT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+LFY GTT+AAAMYI+GA+E++L Y+ P AS+FG    D + M+NN RVYG +LL  M   VF+G+KFV+KF+T++LACV+ SI+ VY+GIF   + +  L +C+LG RLL + + L+        CTK+ +  L  +YC +G  +      +Y+  NNVT+   I G    +  D  F ++   KG    YG+   +     G+S +  ++DI  SFT+LIGIFFPSVTGIMAGSNRSGDLA+ Q SIP+G+I A  TTS VYLS+V+FF+ TV++ +LRDKFG S+G  L+VA +AWPN+WVILIGSFLST+GAGLQSLTGAPRLLQAIA+D +IPFL  F  ++KRGEP RA  LT  I   G+L+ ++D +APL++MFFLMCYGFVNLA A+   L+ P+WRPRFK+YHW+ SL+GL LCI++M ++SWY+AL+A+ +A  IYKYIEYRGA KEWGD  RG  ++AA+ ++L+L + P HTKNWRPQIL+L  L++      + K+  F +QLKAG+GLTI  +VI+G+     + ++ AK TL + + D+++KG  +++VA  + E +  + QT GLG ++ N V++ +P  W  +  +  WK F+ T+R   A  +A++VPKGI+ +P    K             + G +D+W IVHDGGLL+LL  LL + R W  C+LR+F VA  +DN+I +KK++  F+Y LRI A VEVVE++ +DISAY ++RT+ +  R ++L  + L   +  +  Q I+D H+ A            T+  + F+  PTI     H++Q  EK     LD   + D                        TP++T  +++ +            A GD     K +EF                            RRMH+A++LNE+I  +S +A L+++NLPGP +      + NYM+++E LT+GL +VL VRG G+EVIT ++
Sbjct:   47 ENQKSSIDPNLYLYDDDLETRPHISTFISSIANYENTI-PAATDPDAKPAAPSARMGTLIGVFLPCIQNIFGVILFIRLTWVVGTAGAVCGFLIVLTCCCVTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGMLFYTGTTLAAAMYIVGAVEIVLTYMAPWASIFGDFTKDADAMYNNFRVYGTLLLIFMGLIVFLGVKFVNKFATVALACVILSIIAVYVGIFDNIHGNEKLYMCVLGKRLL-KDIPLEN-------CTKE-DSFLRDIYCPDGKCE------EYYLANNVTKVKGIKGLASGVFYDNIFPSFLE-KGQFISYGKSAIDIENTSGESYNQIMADITTSFTLLIGIFFPSVTGIMAGSNRSGDLADAQKSIPIGTICAILTTSTVYLSSVMFFAGTVDNLLLRDKFGQSIGGKLVVANIAWPNQWVILIGSFLSTLGAGLQSLTGAPRLLQAIARDEIIPFLAPFAKSSKRGEPTRALLLTIVICQCGILLGNVDLLAPLLSMFFLMCYGFVNLACAVQTLLRTPNWRPRFKFYHWSLSLIGLTLCISVMIMTSWYFALIAMGMAIIIYKYIEYRGAEKEWGDGIRGMALTAARYSLLRLEEGPPHTKNWRPQILVLSKLNDNLLP-KYRKIFSFATQLKAGKGLTICVSVIKGDHTKITNKAVDAKATLRKYMTDEKVKGFCDVLVAQQIGEGLSSVIQTIGLGGMKPNTVIIGWPYSWRQEGRN-SWKTFIQTVRTVAACHMALMVPKGINFYPESNHK-------------IGGNIDIWWIVHDGGLLMLLPFLLKQHRTWRNCKLRIFTVAQIEDNSIQMKKDLKTFLYHLRIEADVEVVEMNNSDISAYTYERTLMMEQRNQMLRALGLNKKENSKVVQTIVDHHYDA----------TKTASKVRFAD-PTIEETQHHDSQNDEKRNSIDLDGPENAD------------------------TPETTSNKDEST----------EKADGDFKSSVKPDEFNV--------------------------RRMHTAIKLNEVIVEKSQDAQLVIMNLPGPPREVRAERERNYMEFLEVLTEGLEKVLMVRGGGREVITIYS 1066

[Close]

BLAST of slc12a-4 vs. Ensembl Fly
Match: kcc (gene:FBgn0261794 transcript:FBtr0343299)

HSP 1 Score: 931.784 bits (2407), Expect = 0.000e+0
Identity = 498/1123 (44.35%), Postives = 713/1123 (63.49%), Query Frame = 3

Query:  231 DDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSL--YGEPGKEPHLDKGQSPS--VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTI-NVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPE-KINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSK--NESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            ++++ + D    LY+++  TRP ++   + +A Y   I P + +        S ++GT++GV+ PC+QNIFGV+ F+RL W++G +G   GF+IV  CCC TMLT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+LFY GTT+AAAMYI+GA+E++L Y+ P AS+FG    D + M+NN RVYG +LL  M   VF+G+KFV+KF+T++LACV+ SI+ VY+GIF   + +  L +C+LG RLL + + L+        CTK+ +  L  +YC +G  +      +Y+  NNVT+   I G    +  D  F ++   KG    YG+   +     G+S +  ++DI  SFT+LIGIFFPSVTGIMAGSNRSGDLA+ Q SIP+G+I A  TTS VYLS+V+FF+ TV++ +LRDKFG S+G  L+VA +AWPN+WVILIGSFLST+GAGLQSLTGAPRLLQAIA+D +IPFL  F  ++KRGEP RA  LT  I   G+L+ ++D +APL++MFFLMCYGFVNLA A+   L+ P+WRPRFK+YHW+ SL+GL LCI++M ++SWY+AL+A+ +A  IYKYIEYRGA KEWGD  RG  ++AA+ ++L+L + P HTKNWRPQIL+L  L++      + K+  F +QLKAG+GLTI  +VI+G+     + ++ AK TL + + D+++KG  +++VA  + E +  + QT GLG ++ N V++ +P  W  +  +  WK F+ T+R   A  +A++VPKGI+ +P    K             + G +D+W IVHDGGLL+LL  LL + R W  C+LR+F VA  +DN+I +KK++  F+Y LRI A VEVVE++ +DISAY ++RT+ +  R ++L  + L   +  +  Q I+D H+ A            T+  + F+  PTI     H++Q  EK     LD   + D                        TP++T  +++ +            A GD     K +EF                            RRMH+A++LNE+I  +S +A L+++NLPGP +      + NYM+++E LT+GL +VL VRG G+EVIT ++
Sbjct:   55 ENQKSSIDPNLYLYDDDLETRPHISTFISSIANYENTI-PAATDPDAKPAAPSARMGTLIGVFLPCIQNIFGVILFIRLTWVVGTAGAVCGFLIVLTCCCVTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGMLFYTGTTLAAAMYIVGAVEIVLTYMAPWASIFGDFTKDADAMYNNFRVYGTLLLIFMGLIVFLGVKFVNKFATVALACVILSIIAVYVGIFDNIHGNEKLYMCVLGKRLL-KDIPLEN-------CTKE-DSFLRDIYCPDGKCE------EYYLANNVTKVKGIKGLASGVFYDNIFPSFLE-KGQFISYGKSAIDIENTSGESYNQIMADITTSFTLLIGIFFPSVTGIMAGSNRSGDLADAQKSIPIGTICAILTTSTVYLSSVMFFAGTVDNLLLRDKFGQSIGGKLVVANIAWPNQWVILIGSFLSTLGAGLQSLTGAPRLLQAIARDEIIPFLAPFAKSSKRGEPTRALLLTIVICQCGILLGNVDLLAPLLSMFFLMCYGFVNLACAVQTLLRTPNWRPRFKFYHWSLSLIGLTLCISVMIMTSWYFALIAMGMAIIIYKYIEYRGAEKEWGDGIRGMALTAARYSLLRLEEGPPHTKNWRPQILVLSKLNDNLLP-KYRKIFSFATQLKAGKGLTICVSVIKGDHTKITNKAVDAKATLRKYMTDEKVKGFCDVLVAQQIGEGLSSVIQTIGLGGMKPNTVIIGWPYSWRQEGRN-SWKTFIQTVRTVAACHMALMVPKGINFYPESNHK-------------IGGNIDIWWIVHDGGLLMLLPFLLKQHRTWRNCKLRIFTVAQIEDNSIQMKKDLKTFLYHLRIEADVEVVEMNNSDISAYTYERTLMMEQRNQMLRALGLNKKENSKVVQTIVDHHYDA----------TKTASKVRFAD-PTIEETQHHDSQNDEKRNSIDLDGPENAD------------------------TPETTSNKDEST----------EKADGDFKSSVKPDEFNV--------------------------RRMHTAIKLNEVIVEKSQDAQLVIMNLPGPPREVRAERERNYMEFLEVLTEGLEKVLMVRGGGREVITIYS 1074

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BLAST of slc12a-4 vs. Ensembl Zebrafish
Match: slc12a5a (solute carrier family 12 member 5a [Source:ZFIN;Acc:ZDB-GENE-120927-3])

HSP 1 Score: 937.176 bits (2421), Expect = 0.000e+0
Identity = 503/1127 (44.63%), Postives = 699/1127 (62.02%), Query Frame = 3

Query:  267 LYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELD----ENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPS------VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTL-------DLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            L+E+E  T P ++++ + LA Y+          E  + E  + K   + ++GT++GVY PCLQNI GV+ FLR+ W++G+ G    F+IV +CC +TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VGI FY GTT A AMYI+G IE++L YIVP+A++F    L+    E  M NN RVYG ++L  M   VF+G+K+V+K + + LACV+CSIL VY G+   A       VC+LG+R L      D  A    R    +   L++L+C   ++ LN     YF  NNVTE   IPG     L++  F NY   KG+   + G    ++    P+      ++DI   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTS +Y+S+V+ F A +E TVLRDKFG +V   L++  LAWP+ WVI+IGSF ST GAGLQSLTGAPRL+QAIA+D ++PFL VF      GEP  A  LT  I   G+LIAS+D++AP+++MFFLMCY FVNLA A+   L+ P+WRPRFK+YHWT S LG+ LC++LMF+ SWYYA+VA+ +AG IYKYIE+RGA KEWGD  RG  +SAA+ A+++L + P HTKNWRPQIL+L+N+D    K+  P+++   SQLKAG+GLTIVGT +EG   +       A+++L + +  +++KG S++V++SN+ +A+  L Q SGLG L+HN V+ S+P  W   + H  W+ F+  +R   A   A+LVPK I  F            PS+G     G +D+W IVHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LRI+A VEVVE+  +DISAY +++T+ +  R +IL+ +NL   + +RE Q+I D             +I   +P        T  + V E +P   ++E   +E+  P     S    +   KS+N       TP S     Q   T    T        + N   G   PE I +            F+  P   + N+     RRMH+A+RLNE+I  +S  A L+L+N+PGP +N S D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:    3 LFEDEMDTIPMVSSLLSSLANYSNLPQGSKEHEEAENNEGSRKKPVQAPRMGTLMGVYLPCLQNILGVILFLRMTWMVGIGGVIEAFIIVLMCCSTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFYLGTTYAGAMYILGCIEILLVYIVPQAAIFKMEGLEGAEAEAAMLNNMRVYGTLVLSFMAIVVFVGVKYVNKLALVFLACVICSILAVYAGVIKTAFEPPVFPVCVLGNRTLVWKG-FDVCAKVIERENATVTTKLWRLFC--DSEFLNATCDTYFTNNNVTEMQGIPGVMSGTLRENLFGNYMD-KGAFIEKTGLSSVVEPDSIPTNTNRYVLADITSFFTMLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTILAITTTSIIYMSSVVLFGACIEGTVLRDKFGEAVRGNLVIGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLMQAIARDGIVPFLRVFGHGKANGEPTWALLLTACICESGILIASLDNVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLSLMFICSWYYAIVAMGIAGCIYKYIEFRGAEKEWGDGIRGLSLSAARFALMRLEEGPPHTKNWRPQILVLVNMDGEL-KVDQPRMLSLTSQLKAGKGLTIVGTALEGTYLENHDQGQQAEQSLRKLMETEKVKGFSQVVISSNMRDAMSHLVQASGLGGLQHNTVLASWPRNWKQAEDHQSWRNFIELVRETTAGSKALLVPKNISAF------------PSNGERFTEGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRIDAAVEVVEMHDSDISAYTYEKTLMMEQRSQILKQINLTKTEREREIQSITDSSR---------GSIRRKNP-----AAVTTQLSVTE-EPAASSKEQKPEEECVPV--SPSQQEQLIHDKSTNPPTP--ATPQSPSADAQTPGTAVQMTWSEVKCDGEQNKPVGATTPEAIKDL-----------FNMKPEWENLNQSNV--RRMHTALRLNEVIIKKSQEAKLVLLNMPGPPRNRSGDENYMEFMEVLTEGLNRVLLVRGGGREVITIYS 1080

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BLAST of slc12a-4 vs. Ensembl Zebrafish
Match: slc12a7a (solute carrier family 12 member 7a [Source:ZFIN;Acc:ZDB-GENE-070912-696])

HSP 1 Score: 909.827 bits (2350), Expect = 0.000e+0
Identity = 490/1151 (42.57%), Postives = 701/1151 (60.90%), Query Frame = 3

Query:  177 ENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYT----GGIEPTSGETKKLKEK---SSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVA--NPSRSLRVCMLGDRLLN---RSVVLDKEANNTFRCTKDINGP--LYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNY--YTT---KGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            E I  + TP     +  +D ++        LYEEE  + P ++++ NKLA YT    G +E    E++   ++   +  ++GT +GV+ PC QNI GV+ FLRL WI+G +G      +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISR+LGPE GG VG+ FY GTT A +MYI+G +E+ L YI+P A++F     +   M NN R+YG   L IM   VF+G+K+V+K S + L+CV+ SI+ +Y G+  +   PS S  +C+LG+R L    +S   DK          +I  P  L+  +C NGT D      +YF  N +TE  A+PG     + +  +  Y  Y T   K ++  E       +   S  V+D+   FT+L+GI+FPSVTGIMAGSNRSGDL +PQ SIP G+I A ATTSF+Y+S V+ F A  E  VLRDK+G SV + L+VA LAWP+ WVI+IGSF S  GAGLQSLTGAPR+LQAIA+D ++PFL+VF  +   GEP  A  LT  I  +G+LIAS+D++AP+++MFFLMCY FVNLA A+   L+ P+WRPRFK+YHW+ S LG+ L ++LMFVSSWYYALV I +AG IYKYIEYRGA KEWGD  RG  ++AA+ A+++L +   HTKNWRPQ+L+L+ LD   T + HP+L+ F SQLKAG+GLTIV +V+EG    + +D+ +A++ +  A+  ++ KG   +VV+SN  +    L Q++GLG ++HN V+V++P  W   +S + WK F+ T+R   A+  A+LV K +D FP  + +               GT+D+W IVHDGGLL+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LR++A+VEVVE++  DISA+ +++T+ +  R ++L+ M L   + +RE Q+I D+  ++                      P      H ++   K     LDE+     +K             NT+    L   +TP  ++  +T+    L     R   A   + +            F+  P    +N      RRMH+AV+LNE++  +S  A L+L+N+PGP KN+  D NYM+++E LT+GL  VL VRG G+EVIT ++
Sbjct:    9 EQILGDETPRETSSLISTDHDDSTYGKNMALYEEEMDSTPMVSSLLNKLANYTNITQGAVEHEEAESEDGIQRVTVNGPQMGTFIGVFLPCTQNILGVILFLRLTWIVGTAGILEALAVVFMCCACTMLTAISMSAIATNGVVPAGGSYYMISRALGPEIGGAVGLCFYLGTTFAGSMYILGTMEIFLTYIMPSAAVFEAN--NPTGMQNNMRIYGTCCLAIMTLVVFVGVKYVNKLSLVFLSCVVLSIMAIYAGVIQSAIKPS-SFTICLLGNRTLQHFGKSGKFDKCMKT--EVIDNITKPTELWYHFC-NGTCD------EYFNLNELTEISAVPGLLSNAITENLWSRYGEYRTTVEKENITSEAALSSEPEVLNSYVVNDMATFFTLLVGIYFPSVTGIMAGSNRSGDLRDPQRSIPTGTIMAIATTSFIYISCVVLFGACFEGVVLRDKYGDSVNKNLVVATLAWPSPWVIVIGSFFSCCGAGLQSLTGAPRILQAIARDGIMPFLQVFGRSKSGGEPTWALLLTAAICEIGILIASLDAVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKFYHWSLSFLGMSLSLSLMFVSSWYYALVVILIAGCIYKYIEYRGAVKEWGDGLRGLSLNAARYALIKLEEAQPHTKNWRPQLLVLMKLDTDLT-VKHPRLLSFTSQLKAGKGLTIVCSVLEGTFMVRGADAKLAEQNIKAAMAKEKTKGFCHVVVSSNQRDGFSHLIQSAGLGGMKHNAVLVAWPANWKQAESSLSWKNFIETVRETTAAHQALLVAKNVDTFPSNQDRLAE------------GTIDVWWIVHDGGLLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLQMFLYHLRLDAKVEVVEMNAGDISAFTYEKTLVMEQRSQMLKQMQLSKTEREREIQSISDESRIS------------------IRRKPCGEKQTHNSKL--KVATVNLDEEAQLIHDK-------------NTKSHATLVEKATPTSDRVHMTWTKEKLIGERNRLRDASMHVRDI-----------FNMKPEW--ENLDQTNVRRMHTAVKLNEVVVKKSKGAQLVLLNMPGPPKNKGGDENYMEFLEVLTEGLDHVLLVRGGGREVITIYS 1088

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BLAST of slc12a-4 vs. Ensembl Zebrafish
Match: slc12a4 (solute carrier family 12 member 4 [Source:ZFIN;Acc:ZDB-GENE-090218-27])

HSP 1 Score: 906.36 bits (2341), Expect = 0.000e+0
Identity = 491/1125 (43.64%), Postives = 690/1125 (61.33%), Query Frame = 3

Query:  252 DSKFDLYEEEFHTRPPMANIFNKLAQYTGGI-------EPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGP--IELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF--VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEAC-KYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSV------SDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            D    L+EEE   RP ++++ ++L  YT          E  S +  + +   S  +GT++GVY PCLQNIFGV+ FLRL WI+G +G    F+IV +CC  TMLT +SMSAIATNG VPAGG+YFMISRSLGPEFGG VG+ FY GTT AAAMYI+GAIE+ LKY++P+A++F P  I      M NN RVYG I L +M   VF+G+K+V+K +++ LACV+ SI+ +Y G    + +P     +CMLG+R L R +  D          + +   L+K +C   + +++   C +YF  NNVTE   IPG    ++K+  + +Y   KG +  E  K P +D   S         +DI  SFT+L+GIFFPS TGIMAGSNRSGDL + Q SIP+G+I A  TTS VY S+V+ F A +E  VLRDKFG +V + L+V  L+WP+ WVI+IGSF STVGAGLQSLTGAPRLLQAIA+DN+IPFL VF      GEP  A  LT  I+ LG+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRF+YYHW  S LG+ +C+ALMF+SSWYYA+VA+ +AG IYKYIEY+GA KEWGD  RG  +SAA+ ++L+L   P HTKNWRPQ+L+L+ LDE    + +P+L+ F SQLKAG+GLTIVG+VI+G   +   ++  +++ +   +  +++KG  ++VVAS V E I  L Q+ GLG ++HN V++ +P  W   +    WK F+ T+R   A+ LA++VPK +  +P    +               G +D+W IVHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LR+ A+VEVVE+  +DISAY ++RT+ +  R ++L+ M L +                                                    E+  E  L +D H           +  L S   E+T+ + P+    +NQ  +T+ +  ++      + APE   E                  +  K  Q+   RRMH+AV+LNE+I ++S +A L+L+N+PGP +N+  D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   72 DRNLALFEEELDIRPKVSSLLSRLVNYTNVTQGAKEHEEAESADASRRQAPKSPNMGTLMGVYLPCLQNIFGVILFLRLTWIVGTAGVVQSFLIVLMCCSCTMLTAISMSAIATNGVVPAGGAYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLKYLIPQAAIFHPADIHAGNGAMLNNMRVYGTICLSLMAVVVFVGVKYVNKLASLFLACVIISIVSIYAGAVKSIFHPPE-FPICMLGNRTLVRDL-FDVCGKTVVVGNETVPSKLWKNFCT--SDNISSAHCDEYFFHNNVTEIKGIPGLASGIIKENMWGDY-MEKGQIL-EKAKLPSVDVHGSMETFGFYVSADIATSFTLLVGIFFPSATGIMAGSNRSGDLRDAQKSIPIGTILAITTTSLVYFSSVVLFGACIEGVVLRDKFGDAVSKNLVVGTLSWPSPWVIVIGSFFSTVGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTGLIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFRYYHWALSFLGMSMCLALMFISSWYYAIVAMGIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYSLLRLEAGPPHTKNWRPQLLVLLKLDEDL-HVKYPRLLTFASQLKAGKGLTIVGSVIQGNFLECYGETQASEQAIKNMMEIERVKGFCQVVVASKVREGIAHLIQSCGLGGMKHNTVVMGWPYGWRQSEDPRAWKTFINTVRCTTAAHLALMVPKNVSFYPSNHERFTD------------GYIDVWWIVHDGGMLMLLPFLLKQHKVWRKCKMRIFTVAQMDDNSIQMKKDLATFLYQLRLEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLKQMRLSS---------------------------------------------------AERDREAQLVKDRHS-------LIRMGSLYSDEEEETIEILPE----KNQ--MTWTSEKIEAERRNRNNAPENFREL-----------------ISIKPDQSNV-RRMHTAVKLNEVIVNKSHDARLVLLNMPGPPRNQEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS 1095

[Close]

BLAST of slc12a-4 vs. Ensembl Zebrafish
Match: slc12a7a (solute carrier family 12 member 7a [Source:ZFIN;Acc:ZDB-GENE-070912-696])

HSP 1 Score: 906.36 bits (2341), Expect = 0.000e+0
Identity = 488/1146 (42.58%), Postives = 699/1146 (60.99%), Query Frame = 3

Query:  192 EATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYT----GGIEPTSGETKKLKEK---SSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVA--NPSRSLRVCMLGDRLLN---RSVVLDKEANNTFRCTKDINGP--LYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNY--YTT---KGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            + TP     +  +D ++        LYEEE  + P ++++ NKLA YT    G +E    E++   ++   +  ++GT +GV+ PC QNI GV+ FLRL WI+G +G      +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISR+LGPE GG VG+ FY GTT A +MYI+G +E+ L YI+P A++F     +   M NN R+YG   L IM   VF+G+K+V+K S + L+CV+ SI+ +Y G+  +   PS S  +C+LG+R L    +S   DK          +I  P  L+  +C NGT D      +YF  N +TE  A+PG     + +  +  Y  Y T   K ++  E       +   S  V+D+   FT+L+GI+FPSVTGIMAGSNRSGDL +PQ SIP G+I A ATTSF+Y+S V+ F A  E  VLRDK+G SV + L+VA LAWP+ WVI+IGSF S  GAGLQSLTGAPR+LQAIA+D ++PFL+VF  +   GEP  A  LT  I  +G+LIAS+D++AP+++MFFLMCY FVNLA A+   L+ P+WRPRFK+YHW+ S LG+ L ++LMFVSSWYYALV I +AG IYKYIEYRGA KEWGD  RG  ++AA+ A+++L +   HTKNWRPQ+L+L+ LD   T + HP+L+ F SQLKAG+GLTIV +V+EG    + +D+ +A++ +  A+  ++ KG   +VV+SN  +    L Q++GLG ++HN V+V++P  W   +S + WK F+ T+R   A+  A+LV K +D FP  + +               GT+D+W IVHDGGLL+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LR++A+VEVVE++  DISA+ +++T+ +  R ++L+ M L   + +RE Q+I D+  ++                      P      H ++   K     LDE+     +K             NT+    L   +TP  ++  +T+    L     R   A   + +            F+  P    +N      RRMH+AV+LNE++  +S  A L+L+N+PGP KN+  D NYM+++E LT+GL  VL VRG G+EVIT ++
Sbjct:   43 DETPRETSSLISTDHDDSTYGKNMALYEEEMDSTPMVSSLLNKLANYTNITQGAVEHEEAESEDGIQRVTVNGPQMGTFIGVFLPCTQNILGVILFLRLTWIVGTAGILEALAVVFMCCACTMLTAISMSAIATNGVVPAGGSYYMISRALGPEIGGAVGLCFYLGTTFAGSMYILGTMEIFLTYIMPSAAVFEAN--NPTGMQNNMRIYGTCCLAIMTLVVFVGVKYVNKLSLVFLSCVVLSIMAIYAGVIQSAIKPS-SFTICLLGNRTLQHFGKSGKFDKCMKT--EVIDNITKPTELWYHFC-NGTCD------EYFNLNELTEISAVPGLLSNAITENLWSRYGEYRTTVEKENITSEAALSSEPEVLNSYVVNDMATFFTLLVGIYFPSVTGIMAGSNRSGDLRDPQRSIPTGTIMAIATTSFIYISCVVLFGACFEGVVLRDKYGDSVNKNLVVATLAWPSPWVIVIGSFFSCCGAGLQSLTGAPRILQAIARDGIMPFLQVFGRSKSGGEPTWALLLTAAICEIGILIASLDAVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKFYHWSLSFLGMSLSLSLMFVSSWYYALVVILIAGCIYKYIEYRGAVKEWGDGLRGLSLNAARYALIKLEEAQPHTKNWRPQLLVLMKLDTDLT-VKHPRLLSFTSQLKAGKGLTIVCSVLEGTFMVRGADAKLAEQNIKAAMAKEKTKGFCHVVVSSNQRDGFSHLIQSAGLGGMKHNAVLVAWPANWKQAESSLSWKNFIETVRETTAAHQALLVAKNVDTFPSNQDRLAE------------GTIDVWWIVHDGGLLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLQMFLYHLRLDAKVEVVEMNAGDISAFTYEKTLVMEQRSQMLKQMQLSKTEREREIQSISDESRIS------------------IRRKPCGEKQTHNSKL--KVATVNLDEEAQLIHDK-------------NTKSHATLVEKATPTSDRVHMTWTKEKLIGERNRLRDASMHVRDI-----------FNMKPEW--ENLDQTNVRRMHTAVKLNEVVVKKSKGAQLVLLNMPGPPKNKGGDENYMEFLEVLTEGLDHVLLVRGGGREVITIYS 1117

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BLAST of slc12a-4 vs. Ensembl Zebrafish
Match: slc12a7a (solute carrier family 12 member 7a [Source:ZFIN;Acc:ZDB-GENE-070912-696])

HSP 1 Score: 905.205 bits (2338), Expect = 0.000e+0
Identity = 489/1146 (42.67%), Postives = 699/1146 (60.99%), Query Frame = 3

Query:  192 EATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYT----GGIEPTSGETKKLKEK---SSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVA--NPSRSLRVCMLGDRLLN---RSVVLDKEANNTFRCTKDINGP--LYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNY--YTT---KGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            + TP     +  +D ++        LYEEE  + P ++++ NKLA YT    G +E    E++   ++   +  ++GT +GV+ PC QNI GV+ FLRL WI+G +G      +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISR+LGPE GG VG+ FY GTT A +MYI+G +E+ L YI+P A++F     +   M NN R+YG   L IM   VF+G+K+V+K S + L+CV+ SI+ +Y G+  +   PS S  +C+LG+R L    +S   DK          +I  P  L+  +C NGT D      +YF  N +TE  A+PG     + +  +  Y  Y T   K ++  E       +   S  V+D+   FT+L+GI+FPSVTGIMAGSNRSGDL +PQ SIP G+I A ATTSF+Y+S V+ F A  E  VLRDK+G SV + L+VA LAWP+ WVI+IGSF S  GAGLQSLTGAPR+LQAIA+D ++PFL+VF  +   GEP  A  LT  I  +G+LIAS+D++AP+++MFFLMCY FVNLA A+   L+ P+WRPRFK+YHW+ S LG+ L ++LMFVSSWYYALV I +AG IYKYIEYRGA KEWGD  RG  ++AA+ A+++L +   HTKNWRPQ+L+L+ LD   T + HP+L+ F SQLKAG+GLTIV +V+EG    + +D+ +A++ +  A+  ++ KG   +VV+SN  +    L Q++GLG ++HN V+V++P  W   +S + WK F+ T+R   A+  A+LV K +D FP  + +               GT+D+W IVHDGGLL+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LR++A+VEVVE++  DISA+ +++T+ +  R ++L+ M L   + +RE Q+I D+  ++                      P      H ++   K     LDE+     +K             NT+    L   +TP  ++  +T+    L     R   A   + +            F+  P       QT   RRMH+AV+LNE++  +S  A L+L+N+PGP KN+  D NYM+++E LT+GL  VL VRG G+EVIT ++
Sbjct:   43 DETPRETSSLISTDHDDSTYGKNMALYEEEMDSTPMVSSLLNKLANYTNITQGAVEHEEAESEDGIQRVTVNGPQMGTFIGVFLPCTQNILGVILFLRLTWIVGTAGILEALAVVFMCCACTMLTAISMSAIATNGVVPAGGSYYMISRALGPEIGGAVGLCFYLGTTFAGSMYILGTMEIFLTYIMPSAAVFEAN--NPTGMQNNMRIYGTCCLAIMTLVVFVGVKYVNKLSLVFLSCVVLSIMAIYAGVIQSAIKPS-SFTICLLGNRTLQHFGKSGKFDKCMKT--EVIDNITKPTELWYHFC-NGTCD------EYFNLNELTEISAVPGLLSNAITENLWSRYGEYRTTVEKENITSEAALSSEPEVLNSYVVNDMATFFTLLVGIYFPSVTGIMAGSNRSGDLRDPQRSIPTGTIMAIATTSFIYISCVVLFGACFEGVVLRDKYGDSVNKNLVVATLAWPSPWVIVIGSFFSCCGAGLQSLTGAPRILQAIARDGIMPFLQVFGRSKSGGEPTWALLLTAAICEIGILIASLDAVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKFYHWSLSFLGMSLSLSLMFVSSWYYALVVILIAGCIYKYIEYRGAVKEWGDGLRGLSLNAARYALIKLEEAQPHTKNWRPQLLVLMKLDTDLT-VKHPRLLSFTSQLKAGKGLTIVCSVLEGTFMVRGADAKLAEQNIKAAMAKEKTKGFCHVVVSSNQRDGFSHLIQSAGLGGMKHNAVLVAWPANWKQAESSLSWKNFIETVRETTAAHQALLVAKNVDTFPSNQDRLAE------------GTIDVWWIVHDGGLLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLQMFLYHLRLDAKVEVVEMNAGDISAFTYEKTLVMEQRSQMLKQMQLSKTEREREIQSISDESRIS------------------IRRKPCGEKQTHNSKL--KVATVNLDEEAQLIHDK-------------NTKSHATLVEKATPTSDRVHMTWTKEKLIGERNRLRDASMHVRDI-----------FNMKPD------QTNV-RRMHTAVKLNEVVVKKSKGAQLVLLNMPGPPKNKGGDENYMEFLEVLTEGLDHVLLVRGGGREVITIYS 1112

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BLAST of slc12a-4 vs. Ensembl Xenopus
Match: slc12a5 (solute carrier family 12 member 5 [Source:NCBI gene;Acc:779759])

HSP 1 Score: 936.021 bits (2418), Expect = 0.000e+0
Identity = 503/1143 (44.01%), Postives = 708/1143 (61.94%), Query Frame = 3

Query:  189 KEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTG---GI----EPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDEN--IMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYY-----TTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            KE++P  +   EM   +  A      L+EEE  T P ++++ + LA YT    G+    E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++GV G    F++V +CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   E  E    + NN RVYG  +L  M   VF+G+K+V+KF+ + L CV+ SI+ +Y G+   A       +C+LG+R L+R    D  A       + +   L+ L+C   +  LN     YF +NNVTE   IPG    L++D  +  Y        K  L   P  +  L+  Q    SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A ATTSFVY+S+VI F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHWT S LG+ LC++LMF+ SWYYALVA+++AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   +       A+E++ R +  +++KG  ++V++SN  + I  L Q+SGLG L+HN ++V +P  W   + H  W+ F+  +R   A+ LA++V K +  FP    +    N            +D+W +VHDGG+L+LL  LL   + W +C +R+F VA   DN+I +KK++  F+Y LRINA+VEVVE+  +DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+            +I   +P          N  +H + P    EE V D +   +E  + ++   +    S + Q+    PD+T  +   + T    T + N+++    PE I +F           F+  P+      Q+   RRMH+AV+LNE+I  +S +A L+L+N+PGP +N   D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   30 KESSPFINSS-EMDTGKNMA------LFEEEMDTSPMVSSLLSGLANYTNLTQGVKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGVGGIMESFIMVVMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKG-FDVCAKFIDSGNETVTTKLWALFC--SSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDA-LNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEESIRRLMEAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGN------------IDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREIQSITDESR---------GSIRRKNPS---------NSRLHLSVP----EEQVGDCEEKSEEEVQLIHDK-NTTGFSESAQSPAEEPDTTQEKVHLTWTKDKCTAEKNSSKCPSTPEAIKDF-----------FNMKPN------QSNV-RRMHTAVKLNEVIVKKSHDAKLVLLNMPGPPRNRKGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS 1107

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BLAST of slc12a-4 vs. Ensembl Xenopus
Match: slc12a5 (solute carrier family 12 member 5 [Source:NCBI gene;Acc:779759])

HSP 1 Score: 935.25 bits (2416), Expect = 0.000e+0
Identity = 503/1143 (44.01%), Postives = 708/1143 (61.94%), Query Frame = 3

Query:  189 KEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTG---GI----EPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDEN--IMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYY-----TTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            KE++P  +   EM   +  A      L+EEE  T P ++++ + LA YT    G+    E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++GV G    F++V +CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   E  E    + NN RVYG  +L  M   VF+G+K+V+KF+ + L CV+ SI+ +Y G+   A       +C+LG+R L+R    D  A       + +   L+ L+C   +  LN     YF +NNVTE   IPG    L++D  +  Y        K  L   P  +  L+  Q    SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A ATTSFVY+S+VI F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHWT S LG+ LC++LMF+ SWYYALVA+++AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   +       A+E++ R +  +++KG  ++V++SN  + I  L Q+SGLG L+HN ++V +P  W   + H  W+ F+  +R   A+ LA++V K +  FP    +    N            +D+W +VHDGG+L+LL  LL   + W +C +R+F VA   DN+I +KK++  F+Y LRINA+VEVVE+  +DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+            +I   +P          N  +H + P    EE V D +   +E  + ++   +    S + Q+    PD+T  +   + T    T + N+++    PE I +F           F+  P+      Q+   RRMH+AV+LNE+I  +S +A L+L+N+PGP +N   D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   23 KESSPFINSS-EMDTGKNMA------LFEEEMDTSPMVSSLLSGLANYTNLTQGVKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGVGGIMESFIMVVMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKG-FDVCAKFIDSGNETVTTKLWALFC--SSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDA-LNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEESIRRLMEAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGN------------IDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREIQSITDESR---------GSIRRKNPS---------NSRLHLSVP----EEQVGDCEEKSEEEVQLIHDK-NTTGFSESAQSPAEEPDTTQEKVHLTWTKDKCTAEKNSSKCPSTPEAIKDF-----------FNMKPN------QSNV-RRMHTAVKLNEVIVKKSHDAKLVLLNMPGPPRNRKGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS 1100

[Close]

BLAST of slc12a-4 vs. Ensembl Xenopus
Match: slc12a5 (solute carrier family 12 member 5 [Source:NCBI gene;Acc:779759])

HSP 1 Score: 934.48 bits (2414), Expect = 0.000e+0
Identity = 503/1143 (44.01%), Postives = 708/1143 (61.94%), Query Frame = 3

Query:  189 KEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTG---GI----EPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDEN--IMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYY-----TTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            KE++P  +   EM   +  A      L+EEE  T P ++++ + LA YT    G+    E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++GV G    F++V +CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   E  E    + NN RVYG  +L  M   VF+G+K+V+KF+ + L CV+ SI+ +Y G+   A       +C+LG+R L+R    D  A       + +   L+ L+C   +  LN     YF +NNVTE   IPG    L++D  +  Y        K  L   P  +  L+  Q    SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A ATTSFVY+S+VI F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHWT S LG+ LC++LMF+ SWYYALVA+++AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   +       A+E++ R +  +++KG  ++V++SN  + I  L Q+SGLG L+HN ++V +P  W   + H  W+ F+  +R   A+ LA++V K +  FP    +    N            +D+W +VHDGG+L+LL  LL   + W +C +R+F VA   DN+I +KK++  F+Y LRINA+VEVVE+  +DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+            +I   +P          N  +H + P    EE V D +   +E  + ++   +    S + Q+    PD+T  +   + T    T + N+++    PE I +F           F+  P+      Q+   RRMH+AV+LNE+I  +S +A L+L+N+PGP +N   D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   20 KESSPFINSS-EMDTGKNMA------LFEEEMDTSPMVSSLLSGLANYTNLTQGVKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGVGGIMESFIMVVMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKG-FDVCAKFIDSGNETVTTKLWALFC--SSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDA-LNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEESIRRLMEAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGN------------IDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREIQSITDESR---------GSIRRKNPS---------NSRLHLSVP----EEQVGDCEEKSEEEVQLIHDK-NTTGFSESAQSPAEEPDTTQEKVHLTWTKDKCTAEKNSSKCPSTPEAIKDF-----------FNMKPN------QSNV-RRMHTAVKLNEVIVKKSHDAKLVLLNMPGPPRNRKGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS 1097

[Close]

BLAST of slc12a-4 vs. Ensembl Xenopus
Match: slc12a5 (solute carrier family 12 member 5 [Source:NCBI gene;Acc:779759])

HSP 1 Score: 912.909 bits (2358), Expect = 0.000e+0
Identity = 488/1114 (43.81%), Postives = 677/1114 (60.77%), Query Frame = 3

Query:  276 EEFHTRPPMANIFNKLAQYTG---GI----EPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDEN--IMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYY-----TTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            EE  T P ++++ + LA YT    G+    E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++GV G    F++V +CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   E  E    + NN RVYG  +L  M   VF+G+K+V+KF+ + L CV+ SI+ +Y G+   A       +C+LG+R L+R    D  A       + +   L+ L+C   +  LN     YF +NNVTE   IPG    L++D  +  Y        K  L   P  +  L+  Q    SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A ATTSFVY+S+VI F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHWT S LG+ LC++LMF+ SWYYALVA+++AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   +       A+E++ R +  +++KG  ++V++SN  + I  L Q+SGLG L+HN ++V +P  W   + H  W+ F+  +R   A+ LA++V K +  FP    +    N            +D+W +VHDGG+L+LL  LL   + W +C +R+F VA   DN+I +KK++  F+Y LRINA+VEVVE+  +DISAY +++T+ +  R +IL+ M+L   + +RE Q I DK+     E                              P E+           PD  +E +  H++  K   T +                          N+++    PE I +F           F+  P+      Q+   RRMH+AV+LNE+I  +S +A L+L+N+PGP +N   D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   23 EEMDTSPMVSSLLSGLANYTNLTQGVKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGVGGIMESFIMVVMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKG-FDVCAKFIDSGNETVTTKLWALFC--SSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDA-LNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEESIRRLMEAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGN------------IDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREVQLIHDKNTTGFSE--------------------------SAQSPAEE-----------PDTTQEKV--HLTWTKDKCTAEK-------------------------NSSKCPSTPEAIKDF-----------FNMKPN------QSNV-RRMHTAVKLNEVIVKKSHDAKLVLLNMPGPPRNRKGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS 1037

[Close]

BLAST of slc12a-4 vs. Ensembl Xenopus
Match: slc12a5 (solute carrier family 12 member 5 [Source:NCBI gene;Acc:779759])

HSP 1 Score: 912.138 bits (2356), Expect = 0.000e+0
Identity = 495/1144 (43.27%), Postives = 689/1144 (60.23%), Query Frame = 3

Query:  189 KEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTG---GI----EPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDEN--IMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYY-----TTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKE-TLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            KE++P  +   EM   +  A      L+EEE  T P ++++ + LA YT    G+    E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++GV G    F++V +CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   E  E    + NN RVYG  +L  M   VF+G+K+V+KF+ + L CV+ SI+ +Y G+   A       +C+LG+R L+R    D  A       + +   L+ L+C   +  LN     YF +NNVTE   IPG    L++D  +  Y        K  L   P  +  L+  Q    SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A ATTSFVY+S+VI F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHWT S LG+ LC++LMF+ SWYYALVA+++AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   +       A+E +++     +++KG  ++V++SN  + I  L Q+SGLG L+HN ++V +P  W   + H  W+ F+  +R   A+ LA++V K +  FP    +    N            +D+W +VHDGG+L+LL  LL   + W +C +R+F VA   DN+I +KK++  F+Y LRINA+VEVVE+  +DISAY +++T+ +  R +IL+ M+L   + +RE Q I DK+     E                              P E+           PD  +E +  H++  K   T +                          N+++    PE I +F           F+  P   + N+     RRMH+AV+LNE+I  +S +A L+L+N+PGP +N   D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   16 KESSPFINSS-EMDTGKNMA------LFEEEMDTSPMVSSLLSGLANYTNLTQGVKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGVGGIMESFIMVVMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYLFPGMAIFIAQEAREEPAALLNNMRVYGTCVLTCMAIVVFVGVKYVNKFALVFLGCVILSIIAIYAGVIKSAFDPPDFPICLLGNRTLSRKG-FDVCAKFIDSGNETVTTKLWALFC--SSPFLNATCDTYFSQNNVTEVQGIPGVASGLIRDNLWAGYMQKGIIVEKQGLRSAPNSDA-LNLDQPYVFSDMTTYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTIMAIATTSFVYISSVILFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLSLMFICSWYYALVAMSIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVVEGTYLENHPQCQRAEEVSISMKYAAEKVKGFCKVVISSNTRDGISHLIQSSGLGGLQHNTLLVGWPRNWRQKEDHQTWRNFIELVRETTAAHLALIVSKNVAMFPGNLERFSEGN------------IDVWWVVHDGGMLMLLPFLLRHHKVWKRCTMRIFTVAQMDDNSIQMKKDLTTFLYHLRINAEVEVVEMHDSDISAYTYEKTLVMEQRSQILKQMHLTKTEREREVQLIHDKNTTGFSE--------------------------SAQSPAEE-----------PDTTQEKV--HLTWTKDKCTAEK-------------------------NSSKCPSTPEAIKDF-----------FNMKPEWENLNQSNV--RRMHTAVKLNEVIVKKSHDAKLVLLNMPGPPRNRKGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS 1058

[Close]

BLAST of slc12a-4 vs. Ensembl Mouse
Match: Slc12a5 (solute carrier family 12, member 5 [Source:MGI Symbol;Acc:MGI:1862037])

HSP 1 Score: 936.406 bits (2419), Expect = 0.000e+0
Identity = 503/1167 (43.10%), Postives = 713/1167 (61.10%), Query Frame = 3

Query:  132 EKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGD---SKFDLYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIEL--DENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEP-------HLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            E   H V + +    E++  +  P        S D E   +       L+EEE  T P ++++ + LA YT          E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G++G    F +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   +   +   M NN RVYG  +L  M + VF+G+K+V+KF+ + L CV+ SIL +Y G+   A    +  +C+LG+R L+R    D  A   +   + +   L+ L+C   ++ LN    +YF  NNVTE   IPG    L+K+  + +Y T KG +    G           +D       SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP G+I A ATTS VY+S+V+ F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAI++D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRF+YYHWT S LG+ LC+ALMF+ SWYYALVA+ +AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   D    +  A+E++ R +  +++KG  ++V++SN+ + +  L Q+ GLG L+HN V+V +P  W   + H  W+ F+  +R   A  LA+LV K +  FP    +               G++D+W IVHDGG+L+LL  LL   + W KC++R+F VA   DN+I +KK++  F+Y LRI A+VEVVE+ ++DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+   + G +             P +P   +NV           EET  D +  P+E  + ++   +    S++               +  +T+   T D + A  +  P        SP +  G+K  FS     +N      RRMH+AVRLNE+I ++S +A L+L+N+PGP +N + D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   22 ESRRHSVADPRRLPREDVKGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHG-FDVCAKLAWEGNETVTTRLWGLFC--SSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLT-KGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSE------------GSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDE---SRGSIRRKN---------PANPRLRLNV----------PEETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGERETDPEVHLTW---TKDKSVAEKNKGP--------SPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS 1138

[Close]

BLAST of slc12a-4 vs. Ensembl Mouse
Match: Slc12a5 (solute carrier family 12, member 5 [Source:MGI Symbol;Acc:MGI:1862037])

HSP 1 Score: 932.939 bits (2410), Expect = 0.000e+0
Identity = 503/1167 (43.10%), Postives = 712/1167 (61.01%), Query Frame = 3

Query:  132 EKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGD---SKFDLYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIEL--DENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEP-------HLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            E   H V + +    E++  +  P        S D E   +       L+EEE  T P ++++ + LA YT          E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G++G    F +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   +   +   M NN RVYG  +L  M + VF+G+K+V+KF+ + L CV+ SIL +Y G+   A    +  +C+LG+R L+R    D  A   +   + +   L+ L+C   ++ LN    +YF  NNVTE   IPG    L+K+  + +Y T KG +    G           +D       SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP G+I A ATTS VY+S+V+ F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAI++D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRF+YYHWT S LG+ LC+ALMF+ SWYYALVA+ +AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   D    +  A+E++ R +  +++KG  ++V++SN+ + +  L Q+ GLG L+HN V+V +P  W   + H  W+ F+  +R   A  LA+LV K +  FP    +               G++D+W IVHDGG+L+LL  LL   + W KC++R+F VA   DN+I +KK++  F+Y LRI A+VEVVE+ ++DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+   + G +             P +P   +NV           EET  D +  P+E  + ++   +    S++               +  +T+   T D + A  +  P        SP +  G+K  FS      N      RRMH+AVRLNE+I ++S +A L+L+N+PGP +N + D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   22 ESRRHSVADPRRLPREDVKGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHG-FDVCAKLAWEGNETVTTRLWGLFC--SSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLT-KGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSE------------GSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDE---SRGSIRRKN---------PANPRLRLNV----------PEETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGERETDPEVHLTW---TKDKSVAEKNKGP--------SPVSSEGIKDFFSMKPNQSNV-----RRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS 1133

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BLAST of slc12a-4 vs. Ensembl Mouse
Match: Slc12a4 (solute carrier family 12, member 4 [Source:MGI Symbol;Acc:MGI:1309465])

HSP 1 Score: 932.554 bits (2409), Expect = 0.000e+0
Identity = 515/1133 (45.45%), Postives = 691/1133 (60.99%), Query Frame = 3

Query:  252 DSKFDLYEEEFHTRPPMANIFNKLAQYTGGI-------EPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDE--NIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF--VANPSRSLRVCMLGDRLLNR--------SVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACK-YFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSL---YGEPGKEPHLDKGQSPS---VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            D    L+EEE   RP ++++  KL  YT          E  SGE  + +   +  +GT++GVY PCLQNIFGV+ FLRL W++G +G     +IV +CCC T+LT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+ FY GTT AAAMYI+GAIE++L YI P A++F P    +  +   NN RVYG I L +M   VF+G+K+V+KF+++ LACV+ SIL +Y G    + +P     VCMLG+R L+R        +VV+D E   T          L+  +C   + +L  ++C  YF  NNVTE P IPG    +L++  + + Y  KG +   +G P  +  L   +S S   V+DI  SFT+L+GIFFPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTS VY S+VI F A +E  VLRDK+G  V   L+V  LAWP+ WVI++GSF ST GAGLQSLTGAPRLLQAIA+DN+IPFL VF      GEP  A  LT  I+ LG+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHWT S LG+ LC+ALMFVSSWYYALVA+ +AG IYKYIEY+GA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ LDE    + +P+L+ F SQLKAG+GLTIVG+VI+G   +   ++  A++T+   +  +++KG  ++VVAS V E +  L Q+ GLG +RHN V++ +P  W   +    WK F+ T+R   A+ LA+LVPK I            A  PS+    + G +D+W IVHDGG+L+LL  LL + + W KC +R+F VA   DN+I +KK++  F+Y LR+ A+VEVVE+  +DISAY ++RT+ +  R ++L  M L    TK                                               TE+  E  L +D H     ES+         S+ E+  +   D   +   R    A P  D ++A     P+   E                    H        RRMH+AV+LNE+I +RS +A L+L+N+PGP KN   D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   65 DRNLALFEEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPP-VFPVCMLGNRTLSRDQFDICAKTVVVDNETVAT---------RLWTFFCH--SPNLTADSCDPYFLLNNVTEIPGIPGAAAGVLQENLW-SAYLEKGEVVEKHGLPSTDT-LGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDL-HVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNI------------AFYPSNHERYLDGHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRL----TK-----------------------------------------------TERDREAQLVKDRHSALRLESL--------YSDEEEESVAGADKIQMTWTRDKYMAEP-WDPSHA-----PDNFRELV------------------HIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS 1087

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BLAST of slc12a-4 vs. Ensembl Mouse
Match: Slc12a5 (solute carrier family 12, member 5 [Source:MGI Symbol;Acc:MGI:1862037])

HSP 1 Score: 932.554 bits (2409), Expect = 0.000e+0
Identity = 501/1148 (43.64%), Postives = 709/1148 (61.76%), Query Frame = 3

Query:  189 KEATPLTDEEIEMSDDEEPAGD---SKFDLYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIEL--DENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEP-------HLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            KE++P  +     S D E   +       L+EEE  T P ++++ + LA YT          E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G++G    F +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   +   +   M NN RVYG  +L  M + VF+G+K+V+KF+ + L CV+ SIL +Y G+   A    +  +C+LG+R L+R    D  A   +   + +   L+ L+C   ++ LN    +YF  NNVTE   IPG    L+K+  + +Y T KG +    G           +D       SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP G+I A ATTS VY+S+V+ F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAI++D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRF+YYHWT S LG+ LC+ALMF+ SWYYALVA+ +AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   D    +  A+E++ R +  +++KG  ++V++SN+ + +  L Q+ GLG L+HN V+V +P  W   + H  W+ F+  +R   A  LA+LV K +  FP    +               G++D+W IVHDGG+L+LL  LL   + W KC++R+F VA   DN+I +KK++  F+Y LRI A+VEVVE+ ++DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+   + G +             P +P   +NV           EET  D +  P+E  + ++   +    S++               +  +T+   T D + A  +  P        SP +  G+K  FS     +N      RRMH+AVRLNE+I ++S +A L+L+N+PGP +N + D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   23 KESSPFIN-----STDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHG-FDVCAKLAWEGNETVTTRLWGLFC--SSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLT-KGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSE------------GSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDE---SRGSIRRKN---------PANPRLRLNV----------PEETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGERETDPEVHLTW---TKDKSVAEKNKGP--------SPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS 1115

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BLAST of slc12a-4 vs. Ensembl Mouse
Match: Slc12a4 (solute carrier family 12, member 4 [Source:MGI Symbol;Acc:MGI:1309465])

HSP 1 Score: 932.169 bits (2408), Expect = 0.000e+0
Identity = 515/1133 (45.45%), Postives = 691/1133 (60.99%), Query Frame = 3

Query:  252 DSKFDLYEEEFHTRPPMANIFNKLAQYTGGI-------EPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDE--NIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF--VANPSRSLRVCMLGDRLLNR--------SVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACK-YFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSL---YGEPGKEPHLDKGQSPS---VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            D    L+EEE   RP ++++  KL  YT          E  SGE  + +   +  +GT++GVY PCLQNIFGV+ FLRL W++G +G     +IV +CCC T+LT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+ FY GTT AAAMYI+GAIE++L YI P A++F P    +  +   NN RVYG I L +M   VF+G+K+V+KF+++ LACV+ SIL +Y G    + +P     VCMLG+R L+R        +VV+D E   T          L+  +C   + +L  ++C  YF  NNVTE P IPG    +L++  + + Y  KG +   +G P  +  L   +S S   V+DI  SFT+L+GIFFPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTS VY S+VI F A +E  VLRDK+G  V   L+V  LAWP+ WVI++GSF ST GAGLQSLTGAPRLLQAIA+DN+IPFL VF      GEP  A  LT  I+ LG+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHWT S LG+ LC+ALMFVSSWYYALVA+ +AG IYKYIEY+GA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ LDE    + +P+L+ F SQLKAG+GLTIVG+VI+G   +   ++  A++T+   +  +++KG  ++VVAS V E +  L Q+ GLG +RHN V++ +P  W   +    WK F+ T+R   A+ LA+LVPK I            A  PS+    + G +D+W IVHDGG+L+LL  LL + + W KC +R+F VA   DN+I +KK++  F+Y LR+ A+VEVVE+  +DISAY ++RT+ +  R ++L  M L    TK                                               TE+  E  L +D H     ES+         S+ E+  +   D   +   R    A P  D ++A     P+   E                    H        RRMH+AV+LNE+I +RS +A L+L+N+PGP KN   D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   63 DRNLALFEEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPP-VFPVCMLGNRTLSRDQFDICAKTVVVDNETVAT---------RLWTFFCH--SPNLTADSCDPYFLLNNVTEIPGIPGAAAGVLQENLW-SAYLEKGEVVEKHGLPSTDT-LGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDL-HVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNI------------AFYPSNHERYLDGHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRL----TK-----------------------------------------------TERDREAQLVKDRHSALRLESL--------YSDEEEESVAGADKIQMTWTRDKYMAEP-WDPSHA-----PDNFRELV------------------HIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS 1085

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BLAST of slc12a-4 vs. UniProt/SwissProt
Match: sp|Q91V14|S12A5_MOUSE (Solute carrier family 12 member 5 OS=Mus musculus OX=10090 GN=Slc12a5 PE=1 SV=2)

HSP 1 Score: 936.406 bits (2419), Expect = 0.000e+0
Identity = 503/1167 (43.10%), Postives = 713/1167 (61.10%), Query Frame = 3

Query:  132 EKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGD---SKFDLYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIEL--DENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEP-------HLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            E   H V + +    E++  +  P        S D E   +       L+EEE  T P ++++ + LA YT          E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G++G    F +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   +   +   M NN RVYG  +L  M + VF+G+K+V+KF+ + L CV+ SIL +Y G+   A    +  +C+LG+R L+R    D  A   +   + +   L+ L+C   ++ LN    +YF  NNVTE   IPG    L+K+  + +Y T KG +    G           +D       SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP G+I A ATTS VY+S+V+ F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAI++D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRF+YYHWT S LG+ LC+ALMF+ SWYYALVA+ +AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   D    +  A+E++ R +  +++KG  ++V++SN+ + +  L Q+ GLG L+HN V+V +P  W   + H  W+ F+  +R   A  LA+LV K +  FP    +               G++D+W IVHDGG+L+LL  LL   + W KC++R+F VA   DN+I +KK++  F+Y LRI A+VEVVE+ ++DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+   + G +             P +P   +NV           EET  D +  P+E  + ++   +    S++               +  +T+   T D + A  +  P        SP +  G+K  FS     +N      RRMH+AVRLNE+I ++S +A L+L+N+PGP +N + D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   22 ESRRHSVADPRRLPREDVKGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHG-FDVCAKLAWEGNETVTTRLWGLFC--SSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLT-KGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSE------------GSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDE---SRGSIRRKN---------PANPRLRLNV----------PEETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGERETDPEVHLTW---TKDKSVAEKNKGP--------SPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS 1138

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BLAST of slc12a-4 vs. UniProt/SwissProt
Match: sp|Q63633|S12A5_RAT (Solute carrier family 12 member 5 OS=Rattus norvegicus OX=10116 GN=Slc12a5 PE=1 SV=2)

HSP 1 Score: 935.636 bits (2417), Expect = 0.000e+0
Identity = 502/1167 (43.02%), Postives = 711/1167 (60.93%), Query Frame = 3

Query:  132 EKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGD---SKFDLYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIEL--DENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEP-------HLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            E   H V + +    E++  +  P        S D E   +       L+EEE  T P ++++ + LA YT          E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G++G    F +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   +   +   M NN RVYG  +L  M + VF+G+K+V+KF+ + L CV+ SIL +Y G+   A    +  +C+LG+R L+R    D  A   +   + +   L+ L+C   ++ LN    +YF  NNVTE   IPG    L+K+  + +Y T KG +    G           +D       SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP G+I A ATTS VY+S+V+ F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAI++D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRF+YYHWT S LG+ LC+ALMF+ SWYYALVA+ +AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   D    +  A+E++ R +  +++KG  ++V++SN+ + +  L Q+ GLG L+HN V+V +P  W   + H  W+ F+  +R   A  LA+LV K +  FP    +               G++D+W IVHDGG+L+LL  LL   + W KC++R+F VA   DN+I +KK++  F+Y LRI A+VEVVE+ ++DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+            +I   +P          N  +  N P    EET  D +  P+E  + ++   +    S++               ++       T D + A+ +  P        SP +  G+K  FS     +N      RRMH+AVRLNE+I ++S +A L+L+N+PGP +N + D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   22 ESRRHSVADPRRLPREDVKGDGNPKESSPFINSTDTEKGREYDGRNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSKEHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHG-FDVCAKLAWEGNETVTTRLWGLFC--SSRLLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLT-KGVIVERRGMPSVGLADGTPVDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLDNHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSE------------GSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESR---------GSIRRKNP---------ANTRLRLNVP----EETACDNEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEK--VHLTWTKDKSAAQKNKGP--------SPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVNKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEQLDRVMLVRGGGREVITIYS 1139

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BLAST of slc12a-4 vs. UniProt/SwissProt
Match: sp|Q9H2X9|S12A5_HUMAN (Solute carrier family 12 member 5 OS=Homo sapiens OX=9606 GN=SLC12A5 PE=1 SV=3)

HSP 1 Score: 933.324 bits (2411), Expect = 0.000e+0
Identity = 501/1167 (42.93%), Postives = 709/1167 (60.75%), Query Frame = 3

Query:  132 EKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGD---SKFDLYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIEL--DENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEP-------HLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            E   H V + +    E++  +  P        S D E   +       L+EEE  T P ++++ + LA YT          E  + E  K K   + ++GT +GVY PCLQNIFGV+ FLRL W++G++G    F +VF+CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L Y+ P  ++F   +   +   M NN RVYG  +L  M + VF+G+K+V+KF+ + L CV+ SIL +Y G+   A    +  +C+LG+R L+R    D  A   +   + +   L+ L+C   ++ LN    +YF  NNVTE   IPG    L+K+  + +Y T KG +    G           +D       SD+   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP G+I A ATTS VY+S+V+ F A +E  VLRDKFG +V   L+V  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAI++D ++PFL+VF      GEP  A  LT  I  +G+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRF+YYHWT S LG+ LC+ALMF+ SWYYALVA+ +AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ +D+    + HP+L+   SQLKAG+GLTIVG+V+EG   +    +  A+E++ R +  +++KG  ++V++SN+ + +  L Q+ GLG L+HN V+V +P  W   + H  W+ F+  +R   A  LA+LV K +  FP    +               G++D+W IVHDGG+L+LL  LL   + W KC++R+F VA   DN+I +KK++  F+Y LRI A+VEVVE+ ++DISAY +++T+ +  R +IL+ M+L   + +RE Q+I D+            +I   +P          N  +  N P    EET  D +  P+E  + ++   +    S++               ++       T D + A  +  P        SP +  G+K  FS     +N      RRMH+AVRLNE+I  +S +A L+L+N+PGP +N + D NYM+++E LT+ L RV+ VRG G+EVIT ++
Sbjct:   22 ESRRHSVADPRHLPGEDVKGDGNPKESSPFINSTDTEKGKEYDGKNMALFEEEMDTSPMVSSLLSGLANYTNLPQGSREHEEAENNEGGKKKPVQAPRMGTFMGVYLPCLQNIFGVILFLRLTWVVGIAGIMESFCMVFICCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLAYLFPAMAIFKAEDASGEAAAMLNNMRVYGTCVLTCMATVVFVGVKYVNKFALVFLGCVILSILAIYAGVIKSAFDPPNFPICLLGNRTLSRHG-FDVCAKLAWEGNETVTTRLWGLFC--SSRFLNATCDEYFTRNNVTEIQGIPGAASGLIKENLWSSYLT-KGVIVERSGMTSVGLADGTPIDMDHPYVFSDMTSYFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPTGTILAIATTSAVYISSVVLFGACIEGVVLRDKFGEAVNGNLVVGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAISRDGIVPFLQVFGHGKANGEPTWALLLTACICEIGILIASLDEVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFRYYHWTLSFLGMSLCLALMFICSWYYALVAMLIAGLIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLVRVDQD-QNVVHPQLLSLTSQLKAGKGLTIVGSVLEGTFLENHPQAQRAEESIRRLMEAEKVKGFCQVVISSNLRDGVSHLIQSGGLGGLQHNTVLVGWPRNWRQKEDHQTWRNFIELVRETTAGHLALLVTKNVSMFPGNPERFSE------------GSIDVWWIVHDGGMLMLLPFLLRHHKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRITAEVEVVEMHESDISAYTYEKTLVMEQRSQILKQMHLTKNEREREIQSITDESR---------GSIRRKNP---------ANTRLRLNVP----EETAGDSEEKPEEEVQLIHDQSAPSCPSSSPSPGEEPEGEGETDPEK--VHLTWTKDKSVAEKNKGP--------SPVSSEGIKDFFSMKPEWENLNQSNVRRMHTAVRLNEVIVKKSRDAKLVLLNMPGPPRNRNGDENYMEFLEVLTEHLDRVMLVRGGGREVITIYS 1139

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BLAST of slc12a-4 vs. UniProt/SwissProt
Match: sp|Q63632|S12A4_RAT (Solute carrier family 12 member 4 OS=Rattus norvegicus OX=10116 GN=Slc12a4 PE=1 SV=1)

HSP 1 Score: 933.324 bits (2411), Expect = 0.000e+0
Identity = 515/1132 (45.49%), Postives = 688/1132 (60.78%), Query Frame = 3

Query:  252 DSKFDLYEEEFHTRPPMANIFNKLAQYTGGI-------EPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDE--NIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIG-IFVANPSRSLRVCMLGDRLLNR--------SVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACK-YFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSL---YGEPGKEPHLDKGQSPS---VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            D    L+EEE   RP ++++  KL  YT          E  SGE  + +   +  +GT++GVY PCLQNIFGV+ FLRL W++G +G     +IV +CCC T+LT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+ FY GTT AAAMYI+GAIE++L YI P A++F P    +  +   NN RVYG I L  M   VF+G+K+V+KF+++ LACV+ SIL +Y+G I  A       VCMLG+R L+R        +VV+D E   T          L+  +C   + +L  ++C  YF  NNVTE P IPG    +L++  + + Y  KG +   +G P  +  L   +S S   V+DI  SFT+L+GIFFPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTS VY S+VI F A +E  VLRDK+G  V   L+V  LAWP+ WVI++GSF ST GAGLQSLTGAPRLLQAIA+DN+IPFL VF      GEP  A  LT  I+ LG+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHW  S LG+ LC+ALMFVSSWYYALVA+ +AG IYKYIEY+GA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ LDE    + +P+L+ F SQLKAG+GLTIVG+VI+G   +   ++  A++T+   +  +++KG  ++VVAS V E +  L Q+ GLG +RHN V++ +P  W   +    WK F+ T+R   A+ LA+LVPK I            A  PS+    + G +D+W IVHDGG+L+LL  LL + + W KC +R+F VA   DN+I +KK++  F+Y LR+ A+VEVVE+  +DISAY ++RT+ +  R ++L  M L    TK                                               TE+  E  L +D H     ES+         S+ E   +   D   +   R    A P  D ++A     P+   E                    H        RRMH+AV+LNE+I +RS +A L+L+N+PGP KN   D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   63 DRNLALFEEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTFMTLVVFVGVKYVNKFASLFLACVIISILSIYVGGIKSAFDPPVFPVCMLGNRTLSRDQFDICAKTVVVDNETVAT---------RLWTFFCH--SPNLTADSCDPYFLLNNVTEIPGIPGAAAGVLQENLW-SAYLEKGEVVEKHGLPSTDT-LGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFVSSWYYALVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDL-HVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMEIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNI------------AFYPSNHERYLEGHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRL----TK-----------------------------------------------TERDREAQLVKDRHSALRLESL--------YSDEEDESVTGADKIQMTWTRDKYMAEP-WDPSHA-----PDNFRELV------------------HIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS 1085

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BLAST of slc12a-4 vs. UniProt/SwissProt
Match: sp|Q9JIS8|S12A4_MOUSE (Solute carrier family 12 member 4 OS=Mus musculus OX=10090 GN=Slc12a4 PE=1 SV=2)

HSP 1 Score: 932.169 bits (2408), Expect = 0.000e+0
Identity = 515/1133 (45.45%), Postives = 691/1133 (60.99%), Query Frame = 3

Query:  252 DSKFDLYEEEFHTRPPMANIFNKLAQYTGGI-------EPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDE--NIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF--VANPSRSLRVCMLGDRLLNR--------SVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACK-YFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSL---YGEPGKEPHLDKGQSPS---VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            D    L+EEE   RP ++++  KL  YT          E  SGE  + +   +  +GT++GVY PCLQNIFGV+ FLRL W++G +G     +IV +CCC T+LT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+ FY GTT AAAMYI+GAIE++L YI P A++F P    +  +   NN RVYG I L +M   VF+G+K+V+KF+++ LACV+ SIL +Y G    + +P     VCMLG+R L+R        +VV+D E   T          L+  +C   + +L  ++C  YF  NNVTE P IPG    +L++  + + Y  KG +   +G P  +  L   +S S   V+DI  SFT+L+GIFFPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTS VY S+VI F A +E  VLRDK+G  V   L+V  LAWP+ WVI++GSF ST GAGLQSLTGAPRLLQAIA+DN+IPFL VF      GEP  A  LT  I+ LG+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHWT S LG+ LC+ALMFVSSWYYALVA+ +AG IYKYIEY+GA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ LDE    + +P+L+ F SQLKAG+GLTIVG+VI+G   +   ++  A++T+   +  +++KG  ++VVAS V E +  L Q+ GLG +RHN V++ +P  W   +    WK F+ T+R   A+ LA+LVPK I            A  PS+    + G +D+W IVHDGG+L+LL  LL + + W KC +R+F VA   DN+I +KK++  F+Y LR+ A+VEVVE+  +DISAY ++RT+ +  R ++L  M L    TK                                               TE+  E  L +D H     ES+         S+ E+  +   D   +   R    A P  D ++A     P+   E                    H        RRMH+AV+LNE+I +RS +A L+L+N+PGP KN   D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   63 DRNLALFEEELDIRPKVSSLLGKLVSYTNLTQGAKEHEEAESGEGGRRRAAKAPSMGTLMGVYLPCLQNIFGVILFLRLTWMVGTAGVLQALLIVLICCCCTLLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAAAMYILGAIEILLTYIAPPAAIFYPSGTHDMSSATLNNMRVYGTIFLTLMTLVVFVGVKYVNKFASLFLACVIISILSIYAGGIKSIFDPP-VFPVCMLGNRTLSRDQFDICAKTVVVDNETVAT---------RLWTFFCH--SPNLTADSCDPYFLLNNVTEIPGIPGAAAGVLQENLW-SAYLEKGEVVEKHGLPSTDT-LGLKESLSLYVVADIATSFTVLVGIFFPSVTGIMAGSNRSGDLRDAQKSIPVGTILAIVTTSLVYFSSVILFGACIEGVVLRDKYGDGVSRNLVVGTLAWPSPWVIVVGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHGKANGEPTWALLLTALIAELGILIASLDMVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFVSSWYYALVAMLIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQLLVLLKLDEDL-HVKYPRLLTFASQLKAGKGLTIVGSVIQGSFLESYGEAQAAEQTIKNMMDIEKVKGFCQVVVASKVREGLAHLIQSCGLGGMRHNSVVLGWPYGWRQSEDPRAWKTFIDTVRCTTAAHLALLVPKNI------------AFYPSNHERYLDGHIDVWWIVHDGGMLMLLPFLLRQHKVWKKCRMRIFTVAQMDDNSIQMKKDLAIFLYHLRLEAEVEVVEMHNSDISAYTYERTLMMEQRSQMLRQMRL----TK-----------------------------------------------TERDREAQLVKDRHSALRLESL--------YSDEEEESVAGADKIQMTWTRDKYMAEP-WDPSHA-----PDNFRELV------------------HIKPDQSNVRRMHTAVKLNEVIVTRSHDARLVLLNMPGPPKNSEGDENYMEFLEVLTEGLERVLLVRGGGREVITIYS 1085

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BLAST of slc12a-4 vs. TrEMBL
Match: A0A0H3YK51 (Slc12a-4 OS=Schmidtea mediterranea OX=79327 GN=slc12a-4 PE=2 SV=1)

HSP 1 Score: 2295.78 bits (5948), Expect = 0.000e+0
Identity = 1121/1133 (98.94%), Postives = 1121/1133 (98.94%), Query Frame = 3

Query:  177 ENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE 3575
            ENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGLLLLLAHLLIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLK            QTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE
Sbjct:   11 ENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGLLLLLAHLLIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLK------------QTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE 1131

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BLAST of slc12a-4 vs. TrEMBL
Match: A0A5K4F357 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 1201.42 bits (3107), Expect = 0.000e+0
Identity = 619/1145 (54.06%), Postives = 797/1145 (69.61%), Query Frame = 3

Query:  252 DSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEK----SSNK-LGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWAND--KSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSP-DLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDS----TPLQNQR---SVTFA-APTLDLNNARGDMAPEK----------INEFTFSP-----------GAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE 3575
            +++ +L+EE+  T PPM+N F KLA YTGGIEPT GETK+LKEK    S NK LGTILGV+ PC QNIFG+L F+R+ WI GV+G    F+IV +CC  TMLT LSMSAIATNGKVPAGGSYFMISRS+GP FGG VGILFY GTT+A+AMY++GA+EV LKYI P+ASLFG I  D   +FNNTRVYG ILL  +M CVF+GI+FVS+F+ +SLA VL SILCVY+G+F  NPSRS  VC LG RLL++  +     N T +C K+  GP+Y+ YC N  +   +E+C +F  NN++ FPA+PG       + FF+++Y  KG  Y           GQ P+++D+  SF +L+GI+FPSVTGIMAGSNRSGDL NPQ SIP+G+I A   TS VYLS+ + F+A  + +V+RDKFG S G  L+VA  AWP+ WVILIGS LST+GAGLQSLTGAPRLLQAIAQDNV+PFL+VFKV TKRGEPLRAQ L + I+ LG+LIASID + PLITMFFLMCYGFVNLAT LNGFL+EPSWRPRF++YHW  S +GL LCIALMF+SSWYY +VA ALA  IYKYIE+RGA+KEWGDATRG QMS AK+AIL+LG+KP+HTKNWRPQIL+ + LDE   +  H +L+D   QLKAG GLT+V +++EG++ D+++D I AK  L+  I+D +IKGL+E++VAS + E +K + Q +GLGNLRHN +MVS+PE+W  D   S  K  +F++TLR  QA D+A+LVPKGID FP  K              +M G VD+WCIVHDGGLLLL ++LL+R R W KC LR+FVVA+E+D+ + LKK++ +F+YDLRINA VEVV +  ADISAY  QRT  +  R+ +L  M + TV+ + + Q I+D+H   +          D +P D+  S     ++D H+  P      T    D  PD      N+  S   S    +   L PD+     P+ N     +V+FA    + ++ +     P++          INEFTFSP               G K ++  +  H  R T+T RR+H AVRLNE++R+ SS + L++VN+PGPS+   S+Y YM Y+ETLTDGL+R+L VRGTG+EVITAF+E
Sbjct:   54 NTQLNLFEEDDKTLPPMSNWFGKLAAYTGGIEPTVGETKQLKEKQTKSSDNKRLGTILGVFLPCCQNIFGILLFVRVGWITGVAGAFQTFLIVLLCCSCTMLTALSMSAIATNGKVPAGGSYFMISRSIGPAFGGAVGILFYLGTTIASAMYLVGAVEVFLKYIFPQASLFGDITSDA-ALFNNTRVYGTILLFTVMCCVFMGIRFVSRFAAVSLAAVLISILCVYLGVFTVNPSRSPYVCALGGRLLSQDFI---TVNGTAQCHKNTTGPIYEAYCNN-PEIATEESCAFFNANNISYFPAMPGLTSQKFFENFFESHYREKGKAYDNVDFPADRKYGQGPNIADVTSSFMLLLGIYFPSVTGIMAGSNRSGDLTNPQKSIPMGTILAITMTSLVYLSSPLLFAAICDGSVMRDKFGESYGGILLVAAFAWPHFWVILIGSCLSTIGAGLQSLTGAPRLLQAIAQDNVMPFLDVFKVITKRGEPLRAQLLCYGIAQLGILIASIDYLTPLITMFFLMCYGFVNLATMLNGFLKEPSWRPRFRFYHWFLSFVGLCLCIALMFISSWYYTIVAWALAFAIYKYIEFRGASKEWGDATRGLQMSTAKEAILKLGNKPIHTKNWRPQILVYLPLDEN-FQARHDRLLDLVYQLKAGHGLTLVASILEGDIIDRRNDMIAAKAHLSDLIQDHRIKGLAEVLVASTIDEGMKNMAQCAGLGNLRHNTLMVSYPEDWRVDCKSSGSKLSKFISTLRAAQACDIAMLVPKGIDSFPLSKGN------------QMIGNVDVWCIVHDGGLLLLTSYLLMRNRVWRKCHLRIFVVATEEDDIVNLKKDMTKFLYDLRINASVEVVAMSTADISAYVAQRTASIEQRRALLMQMKISTVEARCDPQLIVDQHRKPSSA--------DINPADIVKS---NCSIDSHKLLPAISATSTE-SSDQSPDHATNVTNTSSSDSASKEDTKPSNLKPDTESNDKPISNHSHHCTVSFATGSNVAVSESDKSTNPKENDANSELNRGINEFTFSPSNPLTKLVQKAAREAGSKEEYLLNKAHPGRITRTQRRLHCAVRLNELLRTHSSESDLVIVNMPGPSRTLGSEYYYMDYIETLTDGLSRILLVRGTGREVITAFSE 1168

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BLAST of slc12a-4 vs. TrEMBL
Match: A0A094ZXI2 (Solute carrier family 12 member 5 OS=Schistosoma haematobium OX=6185 GN=MS3_06245 PE=4 SV=1)

HSP 1 Score: 1192.56 bits (3084), Expect = 0.000e+0
Identity = 610/1139 (53.56%), Postives = 788/1139 (69.18%), Query Frame = 3

Query:  252 DSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSN-----KLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWAND--KSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHH------VAAGELTMMTNINDTSPDLP-FSPLPTINVDVHENQPTEKTEETVLD----EDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNA--RGDMAPEKINEFTFSP-----------GAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE 3575
            +++ +L+EE+  T PPM+N F KLA YTGGIEPT GETK+LKEK +      +LGTILGV+ PC QNIFG+L F+R+ WI GV+G    F+IVF+CC  TMLT LSMSAIATNGKVPAGGSYFMISRS+GP FGG VGILFY GTT+A+AMY++GA+EV LKYI P+ASLFG I  D   +FNNTRVYG ILL  +M CVF+GI+FVS+F+ +SLA VL SILCVY+G+F  NPSRS  VC LG RLL++  +     N T +C K+  GP+Y+ YC N  +   +E+C++F  NN++ FPA+PG       + FF+++Y  KG  Y           GQ P+++D+  SF +L+GI+FPSVTGIMAGSNRSGDL NPQ SIPLG+I A   TS VYLS+ + F+A  + +V+RDKFG S G  L+VA  AWP+ WVILIGS LST+GAGLQSLTGAPRLLQAIAQDNV+PFL+VFKV TKRGEPLRAQ L + I+ LG+LIASID + PLITMFFLMCYGFVNLA+ LNGFL+EPSWRPRF++YHW  S +GL LCIALMF+SSWYY +VA ALA  IYKYIE+RGA+KEWGDATRG QMS AK+AIL+LG+KP+HTKNWRPQIL+ + LDE   +  H +L+D   QLKAG GLT+V +++EG++ D+++D I  K  L+  I+  +IKGL+E++VAS + E +K + Q +GLGNLRHN +MVS+PE+W  D   S  K  +F++TLR  QA D+A+LVPKGID FP  K              +M G VD+WCIVHDGGLLLL ++LL+R R W KC LR+FVVA+E D+ + LKK++ +F+YDLRINA VEVV +  ADISAY  QRT  +  R+ +L  M +  V+ + + Q I+D+H       +   ++       D+   LP  S   T + D   +  T  T  +  D    ED  P   K    S+   + + +   T+     S    ++   +      D N+   RG      INEFTFSP               G K +   +  H  + T+T RR+H AVRLNE++R+ SS + L++VN+PGPS+   S+Y YM Y+ETLTDGL+R+L VRGTG+EVITAF+E
Sbjct:   54 NTQLNLFEEDDKTLPPMSNWFGKLAAYTGGIEPTVGETKQLKEKQTKSCDNKRLGTILGVFLPCCQNIFGILLFVRVGWITGVAGAFQTFLIVFLCCSCTMLTALSMSAIATNGKVPAGGSYFMISRSIGPAFGGAVGILFYLGTTIASAMYLVGAVEVFLKYIFPQASLFGDITSDA-ALFNNTRVYGTILLFTVMCCVFMGIRFVSRFAAVSLAAVLISILCVYLGVFTVNPSRSPYVCALGGRLLSQDFI---TVNGTAQCHKNTTGPIYEAYCNN-REIATEESCEFFNANNISYFPAMPGLTSKKFFENFFESHYREKGEAYDSIDFPADRKYGQGPNIADVTSSFMLLLGIYFPSVTGIMAGSNRSGDLTNPQKSIPLGTILAITMTSLVYLSSPLLFAAICDGSVMRDKFGESYGGILLVAAFAWPHFWVILIGSCLSTIGAGLQSLTGAPRLLQAIAQDNVMPFLDVFKVITKRGEPLRAQLLCYGIAQLGILIASIDHLTPLITMFFLMCYGFVNLASMLNGFLKEPSWRPRFRFYHWFLSFVGLCLCIALMFISSWYYTIVAWALAFAIYKYIEFRGASKEWGDATRGLQMSTAKQAILKLGNKPIHTKNWRPQILVYLPLDEN-FQARHDRLLDLVYQLKAGHGLTLVASILEGDIIDRRNDMIAVKSHLSDLIQQHRIKGLAEVLVASTIDEGMKNMAQCAGLGNLRHNTLMVSYPEDWRVDCKSSGSKLSKFISTLRAAQACDIAMLVPKGIDSFPLSKGN------------QMIGNVDVWCIVHDGGLLLLTSYLLMRNRVWRKCHLRIFVVATEDDDIVNLKKDMTKFMYDLRINASVEVVAMSTADISAYVTQRTASIEQRRALLMQMKISNVEARCDPQLIVDQHRKPSSVDINPADIIKSNCSIDSHKLLPAISATSTESSDQSPDHATNVTNTSSSDSASKEDTKPSNMKPDTESNNKSISNHSHHCTVSFATGSNVAVSESDKSTNPKENDTNSELNRG------INEFTFSPSNPLTKLVQKAAKEAGSKEEHLLNKAHPGKITRTQRRLHCAVRLNELLRTHSSESDLVIVNMPGPSRTLGSEYYYMDYIETLTDGLSRILLVRGTGREVITAFSE 1168

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BLAST of slc12a-4 vs. TrEMBL
Match: A0A4Z2DI78 (Solute carrier family 12 member 5 OS=Schistosoma japonicum OX=6182 GN=EWB00_000672 PE=4 SV=1)

HSP 1 Score: 1181.78 bits (3056), Expect = 0.000e+0
Identity = 612/1133 (54.02%), Postives = 781/1133 (68.93%), Query Frame = 3

Query:  300 MANIFNKLAQYTGGIEPTSGETKKLKEKSSN-----KLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWAND--KSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDE--------NKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAA--------PTLDLNNARGDMAPEKINEFTFSPG------------------AMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE 3575
            M+N F KLA YTGGIEPT GETK+L+EK +      +LGTILGV+ PC QNIFG+L F+R+ WI GV+G    F+IV +CC  TMLT LSMSAIATNGKVPAGGSYFMISRS+GP FGG VGILFY GTT+A+AMY++GA+EV LKYI P+ASLFG I  D   +FNNTRVYG ILL I+M CVF+GI+FVS+F+ +SLA VL SI+CVY+GIF  N SRS  VC LG RLL++  V     N T  C K++ GP+Y +YC N  +   DE+C +F  NN++ FPA+PG       + F Q+YY  KG  Y           GQ P+++D+  SF +L+GI+FPSVTGIMAGSNRSGDL NPQ SIPLG+I A A TSFVYLS+ + F+A  + +V+RDKFG S G  L+VA  AWP+ WVILIGS LST+GAGLQSLTGAPRLLQAIAQDNV+PFL VFKV TKRGEPLRAQ L + I+ LG+LIASID + PLITMFFLMCYGFVNLAT LNGFL+EPSWRPRF++YHW  S +GL LCIALMF+SSWYY +VA ALA  IYKYIE+RGA+KEWGDATRG QMS AK+AIL+LG+KP+HTKNWRPQIL+ + LDE   +  H +L+D   QLKAG GLT+V +++EG++ ++++D I AK  L+  I++ +IKGL+E++VAS V E +K + Q +GLGNLRHN +MVSFPE+W  D  +S  K  +F++TLR  QA D+A+LVPKGID FP  K              +MTG VD+WCIVHDGGLLLL ++LL+R R W KC LR+FVVA+E D+T+ L+K++ +F+YDLRINA VEVV +   D+SAY  QRT  +  R+ +L  M +  V  + + Q I+D+H          ++ +   PD+  S     +++ H+  P      T    D  PD         +KES     +K  +  T+    +  DS  L +  +V+FA         P    NN   +     INEFTFSP                    +   K +   +  H  + TKT RR+H AVRLNE++R+ SS++ L++VN+PGPS+   S+Y YM Y+ETLTDGL R+L VRGTG+EVITAF+E
Sbjct:    1 MSNWFGKLAAYTGGIEPTVGETKQLREKQTKSNDNKRLGTILGVFLPCCQNIFGILLFVRVGWITGVAGALQTFLIVLLCCSCTMLTALSMSAIATNGKVPAGGSYFMISRSIGPAFGGAVGILFYLGTTIASAMYLVGAVEVFLKYIFPQASLFGDITSDA-ALFNNTRVYGTILLFIVMCCVFMGIRFVSRFAAVSLAAVLISIICVYLGIFTVNSSRSPFVCALGGRLLSQDFV---TVNGTVECHKNVTGPIYDVYCSN-PETATDESCAFFNANNISYFPAMPGLTSKKFFENFLQSYYREKGQAYDNIDFPADRKYGQGPNIADVTSSFMLLLGIYFPSVTGIMAGSNRSGDLTNPQKSIPLGTILAIAMTSFVYLSSPLLFAAICDGSVMRDKFGESYGGILLVAAFAWPHFWVILIGSCLSTIGAGLQSLTGAPRLLQAIAQDNVMPFLNVFKVITKRGEPLRAQLLCYGIAQLGILIASIDHLTPLITMFFLMCYGFVNLATMLNGFLKEPSWRPRFRFYHWFLSFIGLCLCIALMFISSWYYTIVAWALAFAIYKYIEFRGASKEWGDATRGLQMSTAKQAILKLGNKPIHTKNWRPQILVYLPLDEN-FQARHDRLLDLVYQLKAGHGLTLVASILEGDIIERRNDMIAAKAHLSDLIQEHRIKGLAEVLVASTVDEGMKNMAQCAGLGNLRHNTLMVSFPEDWRIDCKRSGSKLSKFISTLRAAQACDIAMLVPKGIDSFPLSKGN------------QMTGNVDVWCIVHDGGLLLLTSYLLMRNRVWRKCHLRIFVVATEDDDTVNLQKDMTKFMYDLRINASVEVVAMSTVDVSAYITQRTASIEQRRALLMQMKITNVQARCDPQLIVDQHRKP-------SSADIYQPDIVKS---NCSIESHKILPAISAASTE-SSDQSPDHATNLTNTSSKESALKECTKPSNLKTDTEPNIKSDSD-LSHHCTVSFATGSTAAVNDPDKSTNNRPENDTNHGINEFTFSPSNPLTKLVQKAAREAAITNNVTSPKEEHLSNKTHPGKITKTQRRLHCAVRLNELLRNHSSDSVLVIVNMPGPSRTLGSEYYYMDYIETLTDGLPRILLVRGTGREVITAFSE 1103

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BLAST of slc12a-4 vs. TrEMBL
Match: A0A4V3SEW3 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005496 PE=4 SV=1)

HSP 1 Score: 1177.16 bits (3044), Expect = 0.000e+0
Identity = 610/1154 (52.86%), Postives = 794/1154 (68.80%), Query Frame = 3

Query:  261 FDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSN-----KLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWAND--KSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHH--VAAGEL--TMMTNINDTSPDLPFSPLPTIN---VDVHENQPT-EKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFA----------------APTLDLNNARGDMAPEK-INEFTFSPGAMHGLKFDFSPSLGHKN-----------------RQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE 3575
              L+E+E  T  PM+N F KLA YTGGIEPT  ETK+L++K +      +LGT+LGV+ PC QNIFG+L F+R+ WI GV+G    F+IV +CC  TMLT LSMSAIATNGKVPAGGSYFMISRS+GPEFGG VG+LFY GTTVA+AMY++GA+EV LKY+ P+ASLFG I  D   +FNNTR+YG ILL ++M CV +GIKFVS+F+ I L  VLCSI+CVY+GIF+ NP+RS  VC LG RLL++  ++    N T+ C+K+  GP+++ YC N  +   +E+C +F  +N++ FPA+PG       + F  +YY  KG  Y +    P  + GQ P+V+D+  SF IL+ I+FPSVTGIMAGSNRSGDLANPQ SIPLG+I A   TSF YLSA +FFSA  +  V+RDKFG S G GL+VA  AWP+ WV+L+G+ LST+GAGLQ LTGAPRLLQAIAQDNV+P L  FKVTT+RGEPLRAQ +++ I+ LG+LIASIDS+ PLITMFFLMCYGFVNLAT LNGFL+EPSWRPRF+  HW  SL+GL LCIALMF+SSWYY +VA A+AG IYKYIEYRGA+KEWGDATRGFQMS A +AIL+LG KP+HTKNWRPQ+L+ + +D+   +  H  L+D   QLKAG GLT+V  +IEG++ ++  D+  AK TL   I+  +IKGL E++V+S + E +K + Q +GLGNLRHN +MVSFPE+W  D  +   K  QF++T++  QA D+A+LV KGID FP  KSK +          +M G+VD+WCIVHDGGLLLL ++LL+R R W KC+LRVFVVASE D+T+ LKK++ +F+YDLRINA V+VV +  ADISAY  QRT ++  R+++L  M L     + + Q I+D+H   ++ GE     + N    +   P + LP I    +    N P+ E   E+ +DE  H    ++ + S+I  L    T        DS     Q SVTFA                +P  D N+   +  P + +NEFTFSP      K   + +L  K                  +++K  RRMHSA RLNE++R+ SS+A L++VN+P PS++  S+Y YM Y+E LT+GL RVL VRGTG+EVITAF+E
Sbjct:   57 LQLFEDEHKTLTPMSNWFGKLAAYTGGIEPTVDETKQLRDKQAKSSERKRLGTVLGVFLPCCQNIFGILLFVRVGWITGVAGALQSFLIVLMCCSCTMLTALSMSAIATNGKVPAGGSYFMISRSIGPEFGGAVGLLFYLGTTVASAMYLVGAVEVFLKYMCPQASLFGDITSD-TALFNNTRIYGTILLILVMCCVLLGIKFVSRFAAIGLVAVLCSIICVYLGIFIVNPARSPYVCALGGRLLSQEFLV---VNGTYDCSKNETGPIHQAYCAN-PETATEESCAFFHNSNLSYFPAMPGISSTKFFENFLPSYYRKKGEAYDDIPFPPKREYGQGPNVADVTTSFMILLAIYFPSVTGIMAGSNRSGDLANPQVSIPLGTITAITVTSFFYLSAPLFFSAICDGAVMRDKFGESYGGGLLVAAFAWPHYWVVLVGTCLSTIGAGLQCLTGAPRLLQAIAQDNVMPMLNPFKVTTRRGEPLRAQLVSYGIAQLGILIASIDSLTPLITMFFLMCYGFVNLATMLNGFLREPSWRPRFRLSHWFLSLIGLGLCIALMFISSWYYTIVAWAIAGAIYKYIEYRGASKEWGDATRGFQMSTATRAILKLGIKPIHTKNWRPQLLVYLPVDDNL-QFRHLGLLDLVHQLKAGHGLTLVVCIIEGDVVERHEDATNAKNTLAELIQQHRIKGLPEVLVSSTISEGMKNMAQCAGLGNLRHNTLMVSFPEDWRADCEQGGRKLSQFISTVKSAQACDVAMLVAKGIDSFP--KSKAE----------QMEGSVDVWCIVHDGGLLLLTSYLLMRNRVWRKCDLRVFVVASEGDDTVNLKKDMTKFMYDLRINAAVDVVAMSTADISAYVAQRTANIEQRREMLMQMKLANTAARYDPQVIVDQHRKSLSGGEPEDVRLPNAGKEATVRPVNELPNIPKVVIAPDSNGPSNEAPPESGVDE-AHKGRPRDELLSNIPLLSQGTTG-------DSEKSSLQCSVTFASGPGPAASARERFLNGSPEGDKNSNLLNTEPNRQLNEFTFSPSHPLARKVAEAAALTAKTNLGNQTSVPPTTPGGGPKRSKMERRMHSAARLNELLRTHSSDADLVIVNMPTPSRSPGSEYYYMDYIEALTEGLTRVLLVRGTGREVITAFSE 1184

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BLAST of slc12a-4 vs. Ensembl Cavefish
Match: slc12a5a (solute carrier family 12 member 5 [Source:NCBI gene;Acc:103035347])

HSP 1 Score: 929.087 bits (2400), Expect = 0.000e+0
Identity = 506/1162 (43.55%), Postives = 705/1162 (60.67%), Query Frame = 3

Query:  189 KEATPLTDEEIEMSDDEEPAGDSKFD-----LYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELD----ENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPS------VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILD--------KHHVAAG-ELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNAR--GDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            KE++P  +         +P    ++D     L+EEE  T P ++++ + LA Y+          E  + E  + K   + ++GT++GVY PCLQNI GV+ FLR+ W++G+ G    FVIV +CC +TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VGI FY GTT A AMYI+G IE++L YIVP+A++F    L+    E  M NN RVYG ++L  M   VF+G+++V+K + + LACV+ SIL VY G+   A       VC LG+R L      D  A    R    +   L++++C   ++ LN     YF  NNVTE   IPG    +LK+  F  Y   KG    + G   +L+  + P+      ++DI   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTSF+Y+S+V+ F A +E TVLRDKFG +V   L++  LAWP+ WVI+ GSF ST GAGLQSLTGAPRLLQAIA+D ++PFL VF      GEP  A  LT  I   G+LIAS+DS+AP+++MFFLMCY FVNLA AL   L+ P+WRPRFK+YHW  S LG+ LC++LMF+ SWYYA+VA+A+A  IYKYIE+RGA KEWGD  RG  +SAA+ A+++L + P HTKNWRPQIL+L+N+D     +  P+L+   +QLKAG+GLTIVGT + G   +    +  A+++L + +  +++KG  ++VV+SN+ +A   L Q SGLG L+HN V+VS+P  W   + H  W+ F+  +R   A+  A+LVPK I  F            PS+G     G +D+W IVHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LRI+AQVEVVE+  +DISAY +++T+ +  R +IL+ +NL   + +RE Q+I D        K+  A   +L++      +S +        +  D   N PT  T ++   E   P                            ST +Q    +T+     D   A+  G   PE I +            F+  P   + N+     RRMH+A+RLN  I  +S  A L+L+N+PGP KN + D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   34 KESSPFIN-------SSDPEKSQQYDGKNMALFEEEMDTSPMVSSLLSSLANYSNLPQGSKEHEEAENNEGTRKKPVQAPRMGTLMGVYLPCLQNILGVILFLRMTWMVGIGGVIEAFVIVLMCCSTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFYLGTTYAGAMYILGCIEILLIYIVPQAAIFKMEGLEGSEAEAAMLNNMRVYGSLVLTFMAIVVFVGVRYVNKLALVFLACVISSILAVYAGVIKTAFEPPLFPVCTLGNRTLVWKG-FDVCAKVIERDNATVTTKLWRMFC--DSEFLNATCDTYFSNNNVTEIQGIPGVTSGILKENLFSTYLE-KGMFLEKKGLSTYLEPDEIPTNSNRYVLADISTFFTMLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTILAITTTSFIYMSSVVLFGACIEGTVLRDKFGEAVRGNLVIGTLAWPSPWVIVFGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLRVFGHGKANGEPTWALLLTAVICESGILIASLDSVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSFLGMSLCLSLMFICSWYYAIVAMAIASCIYKYIEFRGAEKEWGDGIRGLSLSAARFALMRLEEGPPHTKNWRPQILVLVNVDAE-QNVEQPRLLSLTNQLKAGKGLTIVGTALAGTFLENHPQAQQAEQSLKKIMETEKVKGFCQVVVSSNIRDATSHLVQASGLGGLQHNTVLVSWPRNWKQAEDHQLWRNFIELVRETTAASKALLVPKNIAAF------------PSNGERFTEGHIDVWWIVHDGGMLMLLPFLLRQNKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRIDAQVEVVEMQDSDISAYTYEKTLVMEQRSQILKQINLTKTEREREIQSITDSSRGSIRRKNPAAVNTQLSVTEEPAASSKEEKPEEEEQLIHDKSTNPPTPATPQSPAAEAETP----------------------------STVVQ----MTWTEAKCDGEQAKPLGATTPEAIKDL-----------FNMKPEWENLNQFNV--RRMHTALRLNSEIVKKSQEAKLVLLNMPGPPKNRTGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS 1126

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BLAST of slc12a-4 vs. Ensembl Cavefish
Match: slc12a7b (solute carrier family 12 member 7-like [Source:NCBI gene;Acc:103031657])

HSP 1 Score: 927.546 bits (2396), Expect = 0.000e+0
Identity = 498/1157 (43.04%), Postives = 710/1157 (61.37%), Query Frame = 3

Query:  156 ESKVENSENITKEATPLTDEEIEMSDDEEPAGDSK-FDLYEEEFHTRPPMANIFNKLAQYTG---GIEPTSGETKKLKEKS----SNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIE--LDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF--VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNY----YTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLK--FDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            ES     +   KE++P  +      +D     D K   L+EEE  + P ++++ +KLA YT    G+       ++   K     S ++GT +GVY PCLQNI GV+ FLR+ WI+G +G    F+IV +CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L YIVP A++F   +   +   M NN RVYG   L +M   VF+G+K+V+K + + LACV+ SIL +Y G+   +  P     VC+LG+R L ++   DK A         +   L+ L+C   + +LN    +YF  NNVTE P IPG    ++ +  + +Y       + ++      E   D      V+DI   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A ATT+ +YLS V+ F A ++  VLRDKFG SV   L++  L+WP+ WVI+IGSF S  GAGLQSLTGAPRLLQAIA+D ++PFLEVF      GEP  A  LT  I   G+LIAS+D++AP+++MFFLMCY FVNLA AL   L+ P+WRPRFKYYHW  S LG+ LC+ALMF+SSWYYALVA+ +AG IYKYIEYRGA KEWGD  RG  ++AA+ A+++L + P HTKNWRPQ+L+L+NLD    ++ HP+L+ F +QLKAG+GLTIVG+V+EG    K S++  A++ +  A+  ++ KG   +VV+SN+ + I  L Q++GLG ++HN V++++P  W      + WK F+ T+R   A+ LA+LV K +D FP+++   +             GT+D+W IVHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LR++A+VEVVE+ ++DISA+ +++T+ +  R ++L+ M L   + +RE Q+I D+        + +   N T      S           NQ               P E+++   + +   K++ +   L    D+TP +   + T      + N  R              P    G++  F+  P     N+     RRMH+AV+LNE++ ++S  A L+L+N+PGP KN   D NYM+++E L +GL RVL VRG G+EVIT ++
Sbjct:    5 ESSRRQGDGNPKESSPFINNT---DNDRGTLYDGKNMALFEEEMDSNPMVSSLLSKLANYTNLTQGVREHEEADEEEGAKKQTVKSPQMGTFIGVYLPCLQNILGVILFLRMTWIVGTAGILEAFIIVAMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYIVPSAAIFKAEDKAYEAEAMLNNMRVYGTCCLTLMALVVFVGVKYVNKLALVFLACVVLSILAIYAGVIKTIFEPP-VFPVCLLGNRTL-KNHGFDKCAKTDIIGNVTVTTKLWSLFC--DSPELNSTCNEYFTLNNVTEIPGIPGLTSGVISENMWSSYGPAGMLVEKAIDAMEASETTRDVNLPYVVNDIATFFTLLVGIYFPSVTGIMAGSNRSGDLRDAQRSIPIGTILAIATTTIIYLSCVVLFGACIDGVVLRDKFGDSVRGNLVIGTLSWPSPWVIVIGSFFSCCGAGLQSLTGAPRLLQAIARDGIVPFLEVFGHGKANGEPTWALLLTAGICETGILIASLDAVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFISSWYYALVAMLIAGCIYKYIEYRGAEKEWGDGIRGISLNAARYALIRLEEAPPHTKNWRPQLLVLLNLDSEL-RVKHPRLLSFTTQLKAGKGLTIVGSVLEGTYLTKDSEAKKAEQNIKTAMATERTKGFCHVVVSSNLRDGISHLIQSAGLGGMKHNSVLMAWPGSWKQSNDPLSWKNFIETVRETTAAHLALLVAKNVDSFPHQERLGE-------------GTIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLQMFLYHLRLDAEVEVVEMHESDISAFTYEKTLVMEQRSQMLKQMQLSRTEREREIQSITDESR------SSIRRKNQTGAQCSGS----------SNQ-------------GAPLEDEKDNEAQLIHDKNTASHAALNDKADATPDRVHMTWTKEKLFTERNRNR-------------EPNTNMGVRDLFNMKPEWESLNQSNV--RRMHTAVKLNEVVVNKSQGAQLVLLNMPGPPKNRGGDENYMEFLEVLLEGLNRVLLVRGGGREVITIYS 1096

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BLAST of slc12a-4 vs. Ensembl Cavefish
Match: slc12a5a (solute carrier family 12 member 5 [Source:NCBI gene;Acc:103035347])

HSP 1 Score: 926.391 bits (2393), Expect = 0.000e+0
Identity = 502/1156 (43.43%), Postives = 704/1156 (60.90%), Query Frame = 3

Query:  189 KEATPLTDEEIEMSDDEEPAGDSKFD-----LYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELD----ENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPS------VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVA---AGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNAR--GDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            KE++P  +         +P    ++D     L+EEE  T P ++++ + LA Y+          E  + E  + K   + ++GT++GVY PCLQNI GV+ FLR+ W++G+ G    FVIV +CC +TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VGI FY GTT A AMYI+G IE++L YIVP+A++F    L+    E  M NN RVYG ++L  M   VF+G+++V+K + + LACV+ SIL VY G+   A       VC LG+R L      D  A    R    +   L++++C   ++ LN     YF  NNVTE   IPG    +LK+  F  Y   KG    + G   +L+  + P+      ++DI   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTSF+Y+S+V+ F A +E TVLRDKFG +V   L++  LAWP+ WVI+ GSF ST GAGLQSLTGAPRLLQAIA+D ++PFL VF      GEP  A  LT  I   G+LIAS+DS+AP+++MFFLMCY FVNLA AL   L+ P+WRPRFK+YHW  S LG+ LC++LMF+ SWYYA+VA+A+A  IYKYIE+RGA KEWGD  RG  +SAA+ A+++L + P HTKNWRPQIL+L+N+D     +  P+L+   +QLKAG+GLTIVGT + G   +    +  A+++L + +  +++KG  ++VV+SN+ +A   L Q SGLG L+HN V+VS+P  W   + H  W+ F+  +R   A+  A+LVPK I  F            PS+G     G +D+W IVHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LRI+AQVEVVE+  +DISAY +++T+ +  R +IL+ +NL   + +RE Q+I D    +        + T ++ T      S       +     P  + E+ + D+  +P           S    + T  T++             +T+     D   A+  G   PE I +            F+  P   + N+     RRMH+A+RLN  I  +S  A L+L+N+PGP KN + D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   24 KESSPFIN-------SSDPEKSQQYDGKNMALFEEEMDTSPMVSSLLSSLANYSNLPQGSKEHEEAENNEGTRKKPVQAPRMGTLMGVYLPCLQNILGVILFLRMTWMVGIGGVIEAFVIVLMCCSTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFYLGTTYAGAMYILGCIEILLIYIVPQAAIFKMEGLEGSEAEAAMLNNMRVYGSLVLTFMAIVVFVGVRYVNKLALVFLACVISSILAVYAGVIKTAFEPPLFPVCTLGNRTLVWKG-FDVCAKVIERDNATVTTKLWRMFC--DSEFLNATCDTYFSNNNVTEIQGIPGVTSGILKENLFSTYLE-KGMFLEKKGLSTYLEPDEIPTNSNRYVLADISTFFTMLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTILAITTTSFIYMSSVVLFGACIEGTVLRDKFGEAVRGNLVIGTLAWPSPWVIVFGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLRVFGHGKANGEPTWALLLTAVICESGILIASLDSVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSFLGMSLCLSLMFICSWYYAIVAMAIASCIYKYIEFRGAEKEWGDGIRGLSLSAARFALMRLEEGPPHTKNWRPQILVLVNVDAE-QNVEQPRLLSLTNQLKAGKGLTIVGTALAGTFLENHPQAQQAEQSLKKIMETEKVKGFCQVVVSSNIRDATSHLVQASGLGGLQHNTVLVSWPRNWKQAEDHQLWRNFIELVRETTAASKALLVPKNIAAF------------PSNGERFTEGHIDVWWIVHDGGMLMLLPFLLRQNKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRIDAQVEVVEMQDSDISAYTYEKTLVMEQRSQILKQINLTKTEREREIQSITDSSRGSIRRKNPAAVNTQLSVTEEPAASSKEEKPEEECATVSPPPQ-EQLIHDKSTNPPTPATPQ----SPAAEAETPSTVV------------QMTWTEAKCDGEQAKPLGATTPEAIKDL-----------FNMKPEWENLNQFNV--RRMHTALRLNSEIVKKSQEAKLVLLNMPGPPKNRTGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS 1125

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BLAST of slc12a-4 vs. Ensembl Cavefish
Match: slc12a7b (solute carrier family 12 member 7-like [Source:NCBI gene;Acc:103031657])

HSP 1 Score: 926.006 bits (2392), Expect = 0.000e+0
Identity = 497/1146 (43.37%), Postives = 708/1146 (61.78%), Query Frame = 3

Query:  189 KEATPLTDEEIEMSDDEEPAGDSK-FDLYEEEFHTRPPMANIFNKLAQYTG---GIEPTSGETKKLKEKS----SNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIE--LDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF--VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNY----YTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLK--FDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            KE++P  +      +D     D K   L+EEE  + P ++++ +KLA YT    G+       ++   K     S ++GT +GVY PCLQNI GV+ FLR+ WI+G +G    F+IV +CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ FY GTT A AMYI+G IE++L YIVP A++F   +   +   M NN RVYG   L +M   VF+G+K+V+K + + LACV+ SIL +Y G+   +  P     VC+LG+R L ++   DK A         +   L+ L+C   + +LN    +YF  NNVTE P IPG    ++ +  + +Y       + ++      E   D      V+DI   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A ATT+ +YLS V+ F A ++  VLRDKFG SV   L++  L+WP+ WVI+IGSF S  GAGLQSLTGAPRLLQAIA+D ++PFLEVF      GEP  A  LT  I   G+LIAS+D++AP+++MFFLMCY FVNLA AL   L+ P+WRPRFKYYHW  S LG+ LC+ALMF+SSWYYALVA+ +AG IYKYIEYRGA KEWGD  RG  ++AA+ A+++L + P HTKNWRPQ+L+L+NLD    ++ HP+L+ F +QLKAG+GLTIVG+V+EG    K S++  A++ +  A+  ++ KG   +VV+SN+ + I  L Q++GLG ++HN V++++P  W      + WK F+ T+R   A+ LA+LV K +D FP+++   +             GT+D+W IVHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LR++A+VEVVE+ ++DISA+ +++T+ +  R ++L+ M L   + +RE Q+I D+        + +   N T      S         ++  P E  ++  + +  H D+N  S  +   K  +         TPD   +   +   F     + N  R              P    G++  F+  P     N+     RRMH+AV+LNE++ ++S  A L+L+N+PGP KN   D NYM+++E L +GL RVL VRG G+EVIT ++
Sbjct:   69 KESSPFINNT---DNDRGTLYDGKNMALFEEEMDSNPMVSSLLSKLANYTNLTQGVREHEEADEEEGAKKQTVKSPQMGTFIGVYLPCLQNILGVILFLRMTWIVGTAGILEAFIIVAMCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEILLTYIVPSAAIFKAEDKAYEAEAMLNNMRVYGTCCLTLMALVVFVGVKYVNKLALVFLACVVLSILAIYAGVIKTIFEPP-VFPVCLLGNRTL-KNHGFDKCAKTDIIGNVTVTTKLWSLFCD--SPELNSTCNEYFTLNNVTEIPGIPGLTSGVISENMWSSYGPAGMLVEKAIDAMEASETTRDVNLPYVVNDIATFFTLLVGIYFPSVTGIMAGSNRSGDLRDAQRSIPIGTILAIATTTIIYLSCVVLFGACIDGVVLRDKFGDSVRGNLVIGTLSWPSPWVIVIGSFFSCCGAGLQSLTGAPRLLQAIARDGIVPFLEVFGHGKANGEPTWALLLTAGICETGILIASLDAVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFKYYHWALSFLGMSLCLALMFISSWYYALVAMLIAGCIYKYIEYRGAEKEWGDGIRGISLNAARYALIRLEEAPPHTKNWRPQLLVLLNLDSEL-RVKHPRLLSFTTQLKAGKGLTIVGSVLEGTYLTKDSEAKKAEQNIKTAMATERTKGFCHVVVSSNLRDGISHLIQSAGLGGMKHNSVLMAWPGSWKQSNDPLSWKNFIETVRETTAAHLALLVAKNVDSFPHQERLGE-------------GTIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLQMFLYHLRLDAEVEVVEMHESDISAFTYEKTLVMEQRSQMLKQMQLSRTEREREIQSITDESR------SSIRRKNQTGAQCSGSS--------NQGAPLEDEKDNEVAQLIH-DKNTASHAALNDKADA---------TPDRVHMTWTKEKLFT----ERNRNR-------------EPNTNMGVRDLFNMKPEWESLNQSNV--RRMHTAVKLNEVVVNKSQGAQLVLLNMPGPPKNRGGDENYMEFLEVLLEGLNRVLLVRGGGREVITIYS 1150

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BLAST of slc12a-4 vs. Ensembl Cavefish
Match: slc12a5a (solute carrier family 12 member 5 [Source:NCBI gene;Acc:103035347])

HSP 1 Score: 924.465 bits (2388), Expect = 0.000e+0
Identity = 505/1162 (43.46%), Postives = 704/1162 (60.59%), Query Frame = 3

Query:  189 KEATPLTDEEIEMSDDEEPAGDSKFD-----LYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELD----ENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF-VANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPS------VSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILD--------KHHVAAG-ELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNAR--GDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            KE++P  +         +P    ++D     L+EEE  T P ++++ + LA Y+          E  + E  + K   + ++GT++GVY PCLQNI GV+ FLR+ W++G+ G    FVIV +CC +TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VGI FY GTT A AMYI+G IE++L YIVP+A++F    L+    E  M NN RVYG ++L  M   VF+G+++V+K + + LACV+ SIL VY G+   A       VC LG+R L      D  A    R    +   L++++C   ++ LN     YF  NNVTE   IPG    +LK+  F  Y   KG    + G   +L+  + P+      ++DI   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTSF+Y+S+V+ F A +E TVLRDKFG +V   L++  LAWP+ WVI+ GSF ST GAGLQSLTGAPRLLQAIA+D ++PFL VF      GEP  A  LT  I   G+LIAS+DS+AP+++MFFLMCY FVNLA AL   L+ P+WRPRFK+YHW  S LG+ LC++LMF+ SWYYA+VA+A+A  IYKYIE+RGA KEWGD  RG  +SAA+ A+++L + P HTKNWRPQIL+L+N+D     +  P+L+   +QLKAG+GLTIVGT + G   +    +  A+++L + +  +++KG  ++VV+SN+ +A   L Q SGLG L+HN V+VS+P  W   + H  W+ F+  +R   A+  A+LVPK I  F            PS+G     G +D+W IVHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LRI+AQVEVVE+  +DISAY +++T+ +  R +IL+ +NL   + +RE Q+I D        K+  A   +L++      +S +        +  D   N PT  T ++   E   P                            ST +Q    +T+     D   A+  G   PE I +            F+  P+  +        RRMH+A+RLN  I  +S  A L+L+N+PGP KN + D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   34 KESSPFIN-------SSDPEKSQQYDGKNMALFEEEMDTSPMVSSLLSSLANYSNLPQGSKEHEEAENNEGTRKKPVQAPRMGTLMGVYLPCLQNILGVILFLRMTWMVGIGGVIEAFVIVLMCCSTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFYLGTTYAGAMYILGCIEILLIYIVPQAAIFKMEGLEGSEAEAAMLNNMRVYGSLVLTFMAIVVFVGVRYVNKLALVFLACVISSILAVYAGVIKTAFEPPLFPVCTLGNRTLVWKG-FDVCAKVIERDNATVTTKLWRMFC--DSEFLNATCDTYFSNNNVTEIQGIPGVTSGILKENLFSTYLE-KGMFLEKKGLSTYLEPDEIPTNSNRYVLADISTFFTMLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPIGTILAITTTSFIYMSSVVLFGACIEGTVLRDKFGEAVRGNLVIGTLAWPSPWVIVFGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLRVFGHGKANGEPTWALLLTAVICESGILIASLDSVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSFLGMSLCLSLMFICSWYYAIVAMAIASCIYKYIEFRGAEKEWGDGIRGLSLSAARFALMRLEEGPPHTKNWRPQILVLVNVDAE-QNVEQPRLLSLTNQLKAGKGLTIVGTALAGTFLENHPQAQQAEQSLKKIMETEKVKGFCQVVVSSNIRDATSHLVQASGLGGLQHNTVLVSWPRNWKQAEDHQLWRNFIELVRETTAASKALLVPKNIAAF------------PSNGERFTEGHIDVWWIVHDGGMLMLLPFLLRQNKVWRKCKMRIFTVAQMDDNSIQMKKDLTTFLYHLRIDAQVEVVEMQDSDISAYTYEKTLVMEQRSQILKQINLTKTEREREIQSITDSSRGSIRRKNPAAVNTQLSVTEEPAASSKEEKPEEEEQLIHDKSTNPPTPATPQSPAAEAETP----------------------------STVVQ----MTWTEAKCDGEQAKPLGATTPEAIKDL-----------FNMKPNQFNV-------RRMHTALRLNSEIVKKSQEAKLVLLNMPGPPKNRTGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS 1121

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BLAST of slc12a-4 vs. Ensembl Sea Lamprey
Match: slc12a4 (solute carrier family 12 member 4 [Source:ZFIN;Acc:ZDB-GENE-090218-27])

HSP 1 Score: 709.138 bits (1829), Expect = 0.000e+0
Identity = 402/858 (46.85%), Postives = 553/858 (64.45%), Query Frame = 3

Query:  393 KLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIE--LDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIG-IFVANPSRSLRVCMLGDRLL-----NRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVS------DIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPF-LEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAK--ETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKRE 2915
            ++GT +GVY PCLQNIFGV+ FLRL W++G +G    F IVFVCCC TMLT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+ FY GTT AA+MYI+GAIE++L YIVP A++F   +   D   M NN R+YG   L  M   VF+G+K+V+KF+ + LACV+ SIL +Y G +  A       +CMLG+R L     +       + N+T   TK     L+ L+C   +  LN    +YF  NNVTE P IPG     + +  + +Y + KG L  + G E  L  G+ PS S      DI  SFT L+GIFFPSVTGIMAGSNRSGDL + Q SIP+G+I A ATTS VYL++VI F A VE  VLRDK        L++    W + ++ ++ +       G   L+  PR+  +I    + PF ++VF      GEP  A  LT  I+ LG+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHW  S LG+ +C+ALMF+SSWYYA+VA+ +AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ L E   ++  P+L+ F SQLKAG+GLTIVG+V+ G+     +++  A+  +++ R +  +++KG  ++VV+  V + I  L Q+ GLG ++ N V++ +P  W   +    WK F+ T+R   A+  A+LV K +  FP    +               GT+D+W IVHDGG+L+LL  LL + + W KC++R+F VA  +DN+I +KK++  F+Y LRI+A+VEVVE+  +DISAY ++RT+ +  R ++L+ M L   + +RE
Sbjct:    2 RMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLEAFCIVFVCCCCTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAASMYILGAIEILLTYIVPSAAIFRAEDGGSDAAAMLNNMRMYGTAFLAFMAVVVFVGVKYVNKFAMLFLACVILSILAIYAGAVKTAWSPPHFPICMLGNRTLSGKDFDVCAKTTLQENDTLVATK-----LWSLFCD--STFLNASCDEYFDLNNVTEVPGIPGMSSGAILENVWSSY-SGKGDLAEKAGVEA-LGGGEYPSRSLPYVFADIATSFTALVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTIMAIATTSTVYLTSVILFGACVEGVVLRDKHSNPNRNLLLINDFTWVSNFLAVVFTHFDAFQFGTLELS-EPRVSCSIPNVILFPFPIQVFGHGKANGEPTWALLLTAGIAELGILIASLDMVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQVLVLLKLGEG-MEVRAPRLLSFASQLKAGKGLTIVGSVLHGDFLSSCAEAQAAEPHQSIKRLVETEKVKGFYQVVVSPRVRDGISHLIQSCGLGGMKPNTVVMGWPYGWRQSEDPRSWKTFIDTVRATTAAHRALLVAKNVSLFPANSERFAE------------GTIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQLEDNSIQMKKDLAMFLYHLRIDAEVEVVEMHDSDISAYTYERTLMMEQRCQMLKQMRLSKWERERE 836

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BLAST of slc12a-4 vs. Ensembl Sea Lamprey
Match: slc12a4 (solute carrier family 12 member 4 [Source:ZFIN;Acc:ZDB-GENE-090218-27])

HSP 1 Score: 704.131 bits (1816), Expect = 0.000e+0
Identity = 401/856 (46.85%), Postives = 550/856 (64.25%), Query Frame = 3

Query:  393 KLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIE--LDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIG-IFVANPSRSLRVCMLGDRLL-----NRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVS------DIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPF-LEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKRE 2915
            ++GT +GVY PCLQNIFGV+ FLRL W++G +G    F IVFVCCC TMLT +SMSAIATNG VPAGGSYFMISRSLGPEFGG VG+ FY GTT AA+MYI+GAIE++L YIVP A++F   +   D   M NN R+YG   L  M   VF+G+K+V+KF+ + LACV+ SIL +Y G +  A       +CMLG+R L     +       + N+T   TK     L+ L+C   +  LN    +YF  NNVTE P IPG     + +  + +Y + KG L  + G E  L  G+ PS S      DI  SFT L+GIFFPSVTGIMAGSNRSGDL + Q SIP+G+I A ATTS + L++VI F A VE  VLRDKF         +    W + ++ ++ +       G   L+  PR+  +I    + PF ++VF      GEP  A  LT  I+ LG+LIAS+D +AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHW  S LG+ +C+ALMF+SSWYYA+VA+ +AG IYKYIEYRGA KEWGD  RG  +SAA+ A+L+L + P HTKNWRPQ+L+L+ L E   ++  P+L+ F SQLKAG+GLTIVG+V+ G+     +++  A E++ R +  +++KG  ++VV+  V + I  L Q+ GLG ++ N V++ +P  W   +    WK F+ T+R   A+  A+LV K +  FP    +               GT+D+W IVHDGG+L+LL  LL + + W KC++R+F VA  +DN+I +KK++  F+Y LRI+A+VEVVE+  +DISAY ++RT+ +  R ++L+ M L   + +RE
Sbjct:    3 RMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLEAFCIVFVCCCCTMLTAISMSAIATNGVVPAGGSYFMISRSLGPEFGGAVGLCFYLGTTFAASMYILGAIEILLTYIVPSAAIFRAEDGGSDAAAMLNNMRMYGTAFLAFMAVVVFVGVKYVNKFAMLFLACVILSILAIYAGAVKTAWSPPHFPICMLGNRTLSGKDFDVCAKTTLQENDTLVATK-----LWSLFCD--STFLNASCDEYFDLNNVTEVPGIPGMSSGAILENVWSSY-SGKGDLAEKAGVEA-LGGGEYPSRSLPYVFADIATSFTALVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTIMAIATTSRINLTSVILFGACVEGVVLRDKFSCRFCLHSKINDFTWVSNFLAVVFTHFDAFQFGTLELS-EPRVSCSIPNVILFPFPIQVFGHGKANGEPTWALLLTAGIAELGILIASLDMVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKYYHWALSFLGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYRGAEKEWGDGIRGLSLSAARYALLRLEEGPPHTKNWRPQVLVLLKLGEG-MEVRAPRLLSFASQLKAGKGLTIVGSVLHGDFLSSCAEA-QAAESIKRLVETEKVKGFYQVVVSPRVRDGISHLIQSCGLGGMKPNTVVMGWPYGWRQSEDPRSWKTFIDTVRATTAAHRALLVAKNVSLFPANSERFAE------------GTIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQLEDNSIQMKKDLAMFLYHLRIDAEVEVVEMHDSDISAYTYERTLMMEQRCQMLKQMRLSKWERERE 834

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BLAST of slc12a-4 vs. Ensembl Sea Lamprey
Match: slc12a9 (solute carrier family 12 member 9 [Source:NCBI gene;Acc:793341])

HSP 1 Score: 263.077 bits (671), Expect = 9.230e-74
Identity = 200/710 (28.17%), Postives = 337/710 (47.46%), Query Frame = 3

Query:  357 GETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEW--VILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIP-FLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILI-NLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDK-QIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMA 2471
             E    + + S KL T  GV  P L ++F V+ +LR+ +++G +G     ++  V      LTVLS+ AI+TNG V  GG YFMISR+LGPEFGG++G++F+     ++A++++G  E ++     +     P  L +  ++    +Y   LL I +  V +G    SK + I   CV+  +L V+I  F                  NR +V+ K  N T     + +GP            L+D                        LK+ + Q+Y T                         + ++F  +  I F   TGIMAGSN SGDL +P ++IP G+IAA   T  VY+      S +    +L   +            +   N W   + IG + +++ A +  L GA R+L A+A D++    L+V K T++ G P  A  +++F+  L +    +++IA ++T+FFL+ Y  V+LA     +   P++RP F+++ W T LLG++ C+ +MF+ S  YA  +IA+   +  +I YR  +  WG  ++       +K +L L  +  H K WRPQIL+++ N   +C       LI F + +K G GL I+G V  G+L     D + ++     ++ DK  +K   +I ++  V + ++ L + +GLG ++ N + + F ++   D   +    F A
Sbjct:   16 AEAAPPRAEPSGKLNTFFGVVVPTLLSMFSVVLYLRIGFVVGQAGLLESLLMFVVAYVIIGLTVLSVCAISTNGAVQGGGVYFMISRALGPEFGGSIGVMFFLANVCSSAVFLLGLTEAIMDSFRKRPDF--PHGLPDAYIW--VVLYASCLLLICLVVVLVGADIYSKAAFIIFLCVILVLLTVFISFFAVR---------------NRHIVI-KHGNITMNL--NYSGP--------SLGTLHDN-----------------------LKEHYTQDYTT------------------------GVTMTFASVFAIMFNGCTGIMAGSNMSGDLKDPSYAIPRGTIAAVVFTFCVYVLLCFMVSCSCPRDLLLYNY----------VFMRDINVWGPFVTIGIYAASLSAAMSCLIGASRVLHAVALDDIFGVLLKVVKRTSRSGNPWVAVLVSWFLVQLVLFAGQLNTIAGIVTVFFLIAYATVDLACLAMEWASAPNFRPTFQFFSWHTCLLGMVGCLIMMFIISPIYASASIAVMLLLLVFIHYRSPSSSWGYISQALIFHQVRKYLLLLDIRKDHVKFWRPQILLMVANPRSSC------HLIHFVNDIKKG-GLYILGHVEVGDLDSIPYDPLESQVNYWLSLVDKLNVKAFVDITLSPTVRQGVQNLLRIAGLGGMKPNTLFLGFYDDCVPDDYFLSEPAFAA 631

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BLAST of slc12a-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000732.1 (pep scaffold:Pmarinus_7.0:GL479336:3629:33813:1 gene:ENSPMAG00000000665.1 transcript:ENSPMAT00000000732.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 136.732 bits (343), Expect = 1.312e-33
Identity = 120/438 (27.40%), Postives = 197/438 (44.98%), Query Frame = 3

Query: 1545 VILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTT-KRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGD--KPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTV-IEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSH------------IKWKQFMATLRVCQASDLAVLVP-KGIDQFPYRKSKKQSANVPS------------DGPVEMT-----GTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVI 2756
            +I  G F +T+ + L SL  AP++ QA+ +DN+ P +++F V   K  EP+R   LTF I+   +LIA ++ IAP+I+ FFL  Y  +N +       + P WRP FKYY+   SL G  LC  +MFV +W+ ALV   +   +Y Y+ Y+     WG +T+      A    + +    K V     R Q L+L    E     S P L+DF        GL +   V +E      K    + ++         + K     V A ++        Q +GLG ++ N +++ F + W   +S               ++  +   R+ +  D++ +V  + + +F    S K +++               D   +       GT+D+W +  DGGL LL+ +LL   + W  C++RVF+    K N I   K V+
Sbjct:   13 LITAGIFAATLSSALASLVSAPKVFQALCKDNIYPKMQMFAVGYGKNNEPIRGYILTFIIAIAFILIAELNVIAPIISNFFLASYALINFSCFHASLAKSPGWRPAFKYYNMWVSLFGAALCCGVMFVINWWAALVTYVIIFSLYIYVTYQKPDVNWGSSTQRALHGRAVNLCVNVTKLVKDVCACVGR-QCLVLTGPPE-----SRPALVDFVHAFTKNVGLMVCSHVCVELTANASKQHQYLLRQ---------RRKAFYSAVCADDLRGGTLHFLQAAGLGRMKPNTLVIGFKKNWRRAESKDVDEYINIIHDAFDFQYGVLVFRLHEGLDVSHIVQIQVLPKFNEDASHKINSSCCRAVFQTNFDQKLLDASAQFNSRQGKGTIDVWWLFDDGGLTLLVPYLLTTKKTWRDCKIRVFI--GGKINRIDHDKRVM 433

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BLAST of slc12a-4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003053.1 (pep scaffold:Pmarinus_7.0:GL478333:5880:33924:-1 gene:ENSPMAG00000002656.1 transcript:ENSPMAT00000003053.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 136.346 bits (342), Expect = 1.278e-32
Identity = 110/429 (25.64%), Postives = 186/429 (43.36%), Query Frame = 3

Query: 1287 FTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFG--------GSVGEGLIVAKLAWP-----------------------------NEWVILI--GSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVF-KVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIAL----MFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDK 2441
            F  L G+FFP+VTGI+AG+N  GDL +P  +IP G++ A  TT+  YL      + T+   +LRD  G           G   +  +L W                              + W  LI  G F + + + L +L  AP+  Q +A+DN+ P + +F K   +  EPL    L + IS   ++IA ++ IAP+I+ FF++ Y  +N +     F++ P      +       + G+ L        M +S     L  + + G   + +    A   WG + +    + A    L L  +  H KN+RP  L+L     T      P L+DF        GL + G ++ G  G+K  + + +K   N  +  +++       VA+N+ +    L Q++G+G L  N +++ F   W  ++
Sbjct:  303 FFRLFGVFFPAVTGILAGANICGDLKDPASAIPKGTLLAILTTALSYL----IINTTIGAVMLRDASGLLSDSFAWVCNGSSKLACELGWNFTACSLFRPDTTSPCTFGFVNNYQVMAMVSAWAPLIYAGIFGAALSSALANLISAPKCFQGVARDNIYPGISIFGKGFGRNQEPLFGYLLAYLISICFIIIAELNIIAPIISCFFIISYTILNFSCFHASFIKTPG---ETELMQMPIDVEGMQLAAQRLPHSMSLSMSSVELQCVVITGEYQQLLWSLFADVNWGSSMQAMSFNTALYTNLTLDRELYHVKNYRPNCLVL-----TGPPNLRPALVDFVGTFTKDIGLMVCGNILVGPKGEKVKELLESKH--NEWLHRRKVNAFYTGFVATNMQDGAFGLLQSTGIGKLCPNILIMGFKNMWQEEE 717

HSP 2 Score: 107.457 bits (267), Expect = 1.071e-23
Identity = 69/200 (34.50%), Postives = 107/200 (53.50%), Query Frame = 3

Query:  348 PTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFV-ANPSR 944
            P     +++K       G   GV    + NI+GVL +LRL WI   +G  L +VIV V    T ++ LS+SAI+ NGKV  GG+YFM+SR+LG E G   GIL+ F   V  AM+ +G  E  L+ I+  ++L   I +D+    N+ R+ GVI +  ++     G+ + +K   I    ++ S+L   +G  + A P +
Sbjct:   85 PDGEPVEEVKPPEPVLFGWFRGVMMRVIVNIWGVLMYLRLPWITAQAGIGLTWVIVLVAVIITSISGLSISAISNNGKVARGGTYFMVSRTLGYELGAPFGILYSFAAAVGIAMHTVGFAET-LRDILHASNL---IMVDK---VNDIRIIGVITVTFLLGIAMAGLAWENKAQLIFFVLIIISLLNYLLGTVIPATPEK 277

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BLAST of slc12a-4 vs. Ensembl Yeast
Match: VHC1 (Vacuolar membrane cation-chloride cotransporter (CCC); likely mediates K+ and Cl- cotransport into the vacuole; has a role in potassium homeostasis and salt tolerance; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family [Source:SGD;Acc:S000000439])

HSP 1 Score: 169.859 bits (429), Expect = 3.409e-43
Identity = 157/590 (26.61%), Postives = 262/590 (44.41%), Query Frame = 3

Query: 1236 PHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRD-KQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSF-------------------------------------------PEEWANDKSHI---KWKQFMATLRVCQASDLAVL-------VP---------KGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCE-LRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQ 2813
            PHL KG + S+     +F  L G+FFP+  GI AG+  S +L  P  SIP G++     T   Y   V     ++    L D       E  I+  ++   +WVI +G   +++ + +  + GA  +L+AIA+DN+IP LE+F        PL +   T+ ++ L  L + ++ IA  ITM FLM +  +NLA  L G    P++RP FKY++  T+ +G +L +  M +     A V       ++ +I Y    K WGD ++       +K +L+L  +  + K WRPQIL+ ++   T        LI FC+ LK G GL I+G V       K+ + +  ++     IRD   IK   ++    ++I  I+ +   SGLG ++ N  +V F                                           P +   +++ +   +W Q +  L + Q S++A+        +P         K ID +P +   K  A    D P  +T   D + ++      L LA +L+    W +   LRV +   ++ +     + + + +  LRI+A+V VV LDQ
Sbjct:  267 PHLTKGAAGSLLKGKETFNDLFGVFFPATAGIFAGAGMSSELRKPSKSIPKGTLWGLLFTFICYAVVVFSMGCSIPRRSLYD-------EVQIIQTIS-SVQWVIFMGEMATSLFSIIVGMLGAAYVLEAIAKDNIIPGLEIFA-----HSPLYSLIFTWILTQL-CLFSDVNKIATFITMTFLMTFVVMNLACFLLGISSAPNFRPSFKYFNRYTTAIGALLSVVAMLIVDGISASVLFLAMILLFLFIHYFSPPKSWGDVSQSLIYHQVRKYLLRL--RQDNIKYWRPQILLFVDNPRTSW-----NLIRFCNHLKKG-GLYILGHVAVTADFPKQLNELKTQQKAWMKIRDMAAIKAFVQVGTGPSLIWGIRNVFIGSGLGGMKPNITVVGFFDLESYRKHIPQSRSQNNLQKQVEIKATVPRSTCSDVKINVPLPTDECKNETKVNVQQWVQIVEDLSLMQ-SNIAIAHGFKNLEIPNKRDSCFPKKTIDLYPIQMCGKVEAK--GDQPAAITTNFDTYTLI------LQLAAILVTVPEWKRTHSLRVILFVEQEYHRTNETQRMKKLLQVLRIDAEVLVVSLDQ 825

HSP 2 Score: 113.235 bits (282), Expect = 1.157e-25
Identity = 65/187 (34.76%), Postives = 101/187 (54.01%), Query Frame = 3

Query:  384 SSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTR--VYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANP 938
            + +KLGT  GV+ P   N+  +L FLR  +I+G  G      ++ +     +LT LS+SAI+TNG V  GG+Y+MISRSLGPEFGG++G++F+ G    A M  +G IE +L  +   A    P  L E +   +     Y  ++L +  S  F+G + VS+   I    +  SI  + +   + +P
Sbjct:   59 NKDKLGTYDGVFVPTALNVLSILMFLRFGFILGQLGIICTIGLLLLSYTINLLTTLSISAISTNGTVRGGGAYYMISRSLGPEFGGSIGLVFFLGQVFNAGMNAVGIIEPLLYNLGYSAQGEPPAALGELLPRGHWHEFTYATVILFLCFSVAFVGSQTVSRAGNILFLVLAASIFSIPLSALIRSP 245

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BLAST of slc12a-4 vs. Ensembl Nematostella
Match: EDO35443 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLI2])

HSP 1 Score: 738.799 bits (1906), Expect = 0.000e+0
Identity = 434/1076 (40.33%), Postives = 618/1076 (57.43%), Query Frame = 3

Query:  396 LGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQAL--------TFFISFLGVLIASIDSIAPLITM---FFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAIL-----DKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTE-KTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            +GTI+GVY P +QNIFGV+ F+R+ WI+G++G    F IVF+CCC TMLT +SMSA+ATNG VPAGGSYFMISR+LGPEFGG VG+LFY GTT A++MYI+GAIE+++        LF  +EL                                 FS  SL  ++   +      F+    +   VC+LGD +L +S       N++   T   + P++           N    +Y K         +PG    +  +   +++Y  K  +  + G     ++G+  S  D   SF IL+ IFFPSVTGIMAGSNRSGDL + Q SIP G+IAA ATTSFVYL++V+ F AT++  +LRDKFG S+G  L+VA +AWP +WVILIGS LST+GAG+QSLTGAP LLQAIA+DN+IPFL++   T  +    ++ AL        T F+  LG L   +D   P  ++   FFLMCYGFVNLA A+   L+ P+WRPRFKYYHW TS LG+ LC+ALMF+SSWYYALVA+ +A  +YKYIE++GA KEWGD  RG  +SAA+ ++L+L + P HTKNWRPQILIL  LDE+    S  +L+   SQLK G+GL+IVG+V+EGE  +  +D   AKE L   ++++++KG  +IV + NV + I  L Q SGLG L  N V+++FPE W   +    W  F+ T +     + A+LVP+ ID FP             D    + G+VD+W IVHDGG+++L+  LL + + W +C+LR+F VA  +DN+I +KK++  F+Y LRI A+V+V+E+   DIS Y ++RT+ +  R ++L+ M+L   ++KRE QA++      + H +A +       + TS  L  S      V     +P+E +  ET+++E   PD  K+S                                  A+PT  L       AP++ N                        R+  T  +++      ++++ +S  A L+++NLP P  +     NYM +++ LT+GL RVL VRG G+EVIT ++
Sbjct:    1 MGTIMGVYLPTIQNIFGVILFIRMSWIVGIAGVTQAFFIVFICCCCTMLTAISMSAVATNGVVPAGGSYFMISRALGPEFGGAVGLLFYLGTTFASSMYILGAIEILVI-------LFEELEL---------------------------------FSFFSLPYLISRPVASSFSYFLVFTVKFSEVCILGDAILAKSSYETCSYNDSMLRTAYGSDPVF----------WNSTRLRYVK--------GVPGITSGVFTENA-KSHYLKKNEI--KMGVAAGSNEGEIRS--DTTTSFFILLAIFFPSVTGIMAGSNRSGDLKDAQNSIPKGTIAAIATTSFVYLTSVLLFGATIQGELLRDKFGRSIGGVLVVANIAWPTKWVILIGSLLSTIGAGMQSLTGAPCLLQAIAKDNIIPFLDLSTGTLSQLFTQKSAALAKINLLLKTCFVMVLGYL--PLDFCVPGFSLCYGFFLMCYGFVNLACAVQSLLRTPNWRPRFKYYHWFTSFLGVCLCLALMFISSWYYALVAMIIAAAVYKYIEFQGAKKEWGDGIRGLALSAARFSLLRLEEGPPHTKNWRPQILILCKLDESLQPQSR-RLLSLASQLKHGKGLSIVGSVLEGEYQNLVTDITSAKENLKVCMKEEKVKGFMKIVTSENVKQGISFLIQGSGLGGLDPNTVLLAFPENW---RERELWMSFVETTQTVSLGEQALLVPRHIDAFP-------------DNHERLQGSVDVWWIVHDGGMMILILFLLRQHKVWKRCKLRIFTVAQLEDNSIQMKKDLETFMYHLRIEAEVQVIEMVDNDISEYTYERTLVMEQRNQMLKQMHLSRKESKREIQAVVVNSYRSRSHSSASQ---AAEGSRTSSMLDASSSGGSKV---VRRPSEGEAPETIIEE---PDAEKDSEGK-------------------------------ASPTSSLT------APQEQNV-----------------------RRMNTAVKLN------DIVKEKSKEAQLVVINLPAPPTSMDEWQNYMDFLDVLTEGLDRVLMVRGGGREVITIYS 919

[Close]

BLAST of slc12a-4 vs. Ensembl Nematostella
Match: EDO41391 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4G9])

HSP 1 Score: 212.616 bits (540), Expect = 1.882e-56
Identity = 131/408 (32.11%), Postives = 209/408 (51.23%), Query Frame = 3

Query: 1287 FTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFG--GSVGEGLIVAKLAWP----------------------NEWVILI--GSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGE-PLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEW 2429
            F  +  +FFP+ TGI+AG N SGDL + Q +IP G++ A   ++ VY++      A +    LRD  G   +V        +A P                      + W  L+  G F +T+ + L SL GAP+  QA+ +DN+ P++  F +    GE P R   LTF I+   V + +++ IAP+I+ FFLM Y  +N A       + P WRP F+YY+   SL+G +LC+A+MF+ +W+ ALV IA+   ++KY++ +     WG + + F    A +   +L +   H KN+RPQ L+L     T    S P L    SQ+    GL + G V  G L   KS+    K+ L    R+++I+    +  A+++ + ++ L QT+GLG L+ N +++ F   W
Sbjct:  278 FFSVFAVFFPAATGILAGVNISGDLKDAQKAIPKGTLWAILLSTLVYIALAWLAGACI----LRDASGFVETVVNATANVTMATPPSCPGSGCLYGLINDYQAMEKMSAWGPLVTCGIFAATLSSALASLVGAPKTFQALCKDNIFPYIGYFGIGVGPGEEPRRGYILTFIIAVGFVAVGNLNVIAPVISNFFLMSYALINYAVFAASLGRSPGWRPSFRYYNMWVSLVGALLCVAIMFLINWWAALVTIAIIASLHKYVDIKKPEVNWGSSAQAFTYIQALRFAYRLNNTEDHVKNFRPQCLVL-----TGAPSSRPNLTYIVSQITKNVGLMVCGQVNVGSLCQVKSE----KDWL----RERKIRAFHTVCSAASLRDGVQSLLQTAGLGKLKPNTLVIGFKRNW 668

HSP 2 Score: 139.813 bits (351), Expect = 1.638e-33
Identity = 76/193 (39.38%), Postives = 114/193 (59.07%), Query Frame = 3

Query:  381 KSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVC 959
            K + K G + GV   CL NI+GV+ FLRL W++G SG     VI+ +    T +T LSMSA+ TNG+V  GG+Y++ISRSLGPEFGG++G++F     VA A+Y++G  E +   +    SL     +DE    N+ RV GVI +  +++   IG+++V +   + L  +L SI+   +G F+  P   L V 
Sbjct:   69 KMAPKFGWLKGVMLRCLLNIWGVMLFLRLTWVVGQSGIIWSTVIIILSALVTTVTTLSMSAVCTNGEVKGGGAYYLISRSLGPEFGGSIGLIFSLANAVAVALYVVGFAETVRDILRENGSLI----IDE---VNDIRVIGVISVLALLAVTLIGLEWVVRTQMVLLGILLISIVDAIVGSFIG-PQDKLSVA 253

[Close]

BLAST of slc12a-4 vs. Ensembl Nematostella
Match: EDO49361 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG55])

HSP 1 Score: 211.846 bits (538), Expect = 2.350e-56
Identity = 156/564 (27.66%), Postives = 269/564 (47.70%), Query Frame = 3

Query: 1287 FTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLI-------------------VAKLAWPNE-------W--VILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRG-EPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWA--------------ND--------------KSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTI---TLKKEVIRFVYDLRINAQVEV 2798
            F  +  IFFP+ TGI+AG N SGDL NP  ++P G++ A   +S VY    I  +  +  T  RD  G  +   ++                   +  L + N+       W  ++  G F ST+ + + S+ GAP+  QA+ +D + P ++ F V    G EP R   L F I+     I  +++IAP+I+ FFL+ Y  +N AT +    + P WRP F++Y+   SL+G +LC+A+MF+ +W+ ALV I +  G+YK+++YR     WG + +     +A +    L     H KN+RPQ L+L     +      P L+   SQL    GL + G V   +  DK SD    ++   + +++ +IK       A ++   ++ +   +GLG ++ N +++ F  +W               ND              K  I++ +   T  +C   +     P+ + + P +K +++ A    +G  +  GT+D+W +  DGGL +LL +LL   R W  C+LR+F +     + I    LK   +   + +++++ V+V
Sbjct:  275 FFSVFAIFFPAATGILAGVNISGDLKNPHTAVPKGTLLAILVSSLVY----IVLAWLIGATYARDATGLVMSVAVVNGSSQSNVTSIPTCDTMKCLFGLYFDNQAMQKASGWGPIVTAGIFASTLSSAIASIVGAPKTFQAVCKDKLFPKIDYFGVGYGPGNEPKRGYVLAFLIACAFTAIGDLNAIAPIISNFFLIVYALINYATFVASLGRSPGWRPSFRFYNMWVSLIGALLCVAIMFLINWWAALVTIMIVVGLYKFVDYRKPNVNWGSSGQANTYMSALRFTTLLDTHEEHVKNFRPQCLVL-----SGRPAERPDLMYIASQLTKNSGLMMYGNVCRQKF-DKISDDEEREDA--KWLKEHKIKAFRATTTAHSLRTGVQAMLHLTGLGKMKPNTLVLGFKNDWQIAPLADLEGYFGVINDAFQMDFGVAILRIGKETIEFDEVSLTDSICNEDEFK--RPEPVAE-PLQKPEQELA---FEG--KQRGTIDVWWLYDDGGLTILLPYLLTLHRLWRSCDLRLFYLDIRSKHAIKADQLKMANLMKKFRIQVSSVVQV 818

HSP 2 Score: 151.754 bits (382), Expect = 2.469e-37
Identity = 77/184 (41.85%), Postives = 117/184 (63.59%), Query Frame = 3

Query:  381 KSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVA 932
            + + K G I GV+F CL NI+GV+ +LRL W++G +G  L  VI+ +    T +T LSMSAI TNG+V  GG+Y++ISRSLGPEFGG++GI+F   + VA AMY++G  E +   +    +L     +DE    N+ R+ G+I +  +++   +G+K+V +   I LA ++ SIL V IG F+ 
Sbjct:   67 RDAPKFGWIKGVFFGCLLNIWGVMLYLRLSWVVGQAGIGLATVIIMLSAVVTTVTTLSMSAICTNGEVKGGGAYYLISRSLGPEFGGSIGIIFSIASAVAVAMYVVGFAETVRDLLKENGALI----VDE---VNDVRIIGLITIVFILAVALVGLKWVVRTQVILLAVLIISILDVIIGTFIG 243

HSP 3 Score: 55.0694 bits (131), Expect = 1.759e-7
Identity = 23/69 (33.33%), Postives = 44/69 (63.77%), Query Frame = 3

Query: 3366 RRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            +++   +R+ E++R RS+NA L++++LP P    ++   YM ++E L+  L  VL VRG  + V+T ++
Sbjct:  850 KKVLRTIRIGELVRKRSNNAKLVVISLPVPVAEMTTPLMYMSWLEMLSKDLPPVLLVRGNQRSVLTFYS 918

[Close]

BLAST of slc12a-4 vs. Ensembl Nematostella
Match: EDO41917 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S304])

HSP 1 Score: 191.045 bits (484), Expect = 2.272e-51
Identity = 129/402 (32.09%), Postives = 210/402 (52.24%), Query Frame = 3

Query: 1188 NYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGG-SVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVI-----PFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKE----TLNRAIRDKQIKGLSEIVVASNVIEAIKIL 2363
            ++ T K +L+ +  K+   D+    +   ++        I F  VTGIMAG+N SGDL +P  +IP G++ AC  T  VY+  VI    T    +L + +    V + + V         +I IG F ST+ A L  L GA R+L AIA+D ++     PF   F    +  EPL A  L++F   + + I +++++APL++MFFL+CYG  N+A  +      P++RP FKY+ W T+ LG + C  +MF  +  YA ++IA+   ++  I  R     WGD ++       +K +L+L  +  H K WRPQ+L+L++        S   LIDF + +K   GL I+G V   ++G+  SDSI  ++    T    +   +IK   E+ +AS++ + ++ L
Sbjct:  207 SFKTAKSNLWPKFTKDYTKDEHSEQTWLQVF-------AILFNGVTGIMAGANMSGDLKSPGKAIPYGTLTACLGTFVVYVILVICTGFTCSRELLVENYNYLQVIDNVRV---------LITIGVFASTLSAALSCLIGASRILHAIAKDQLLGVILKPFAAGFG---RNNEPLNAVLLSWFFVEIILFIGNLNTVAPLVSMFFLLCYGVTNMACFVLKVASAPNFRPTFKYFSWQTAFLGTVFCFVIMFAINPLYATISIAVMFILFIIISLRAPPTPWGDVSQALIYHQVRKYLLRLDVRKEHVKFWRPQVLLLVS-----NPRSSYNLIDFVNDIKK-SGLYILGHV---QVGEFNSDSICQQKNDTGTWLNFVDCAEIKSFVELTIASSIRQGVQHL 580

HSP 2 Score: 120.553 bits (301), Expect = 4.688e-28
Identity = 69/186 (37.10%), Postives = 106/186 (56.99%), Query Frame = 3

Query:  393 KLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSL 950
            KL    GV  P   ++F V+ FLRL +++G +G  LG  +  V   +  +TVLS+SAIATNG V  GG+Y+MISR+LGPEFGG +GI+F+F    A+A+Y++G +E +L    P+ SL       +   +    +YG  +L + ++   IG    +K S +    V+ S+    + +FV  PS  L
Sbjct:    1 KLSMFFGVIVPVTLSMFSVILFLRLGFVVGQAGLLLGVGLFIVAYLAVSITVLSISAIATNGVVKGGGAYYMISRTLGPEFGGAIGIIFFFANVFASALYVLGFVEALLNNFGPRGSLAHIESFHQGYWYQ--FLYGSAILMLCLAVCMIGAGMFAKTSFVIFLAVMVSVSAALVSLFVDCPSTHL 184

[Close]

BLAST of slc12a-4 vs. Ensembl Nematostella
Match: EDO46430 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQ45])

HSP 1 Score: 94.7449 bits (234), Expect = 8.146e-20
Identity = 62/200 (31.00%), Postives = 109/200 (54.50%), Query Frame = 3

Query: 1230 KEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACA--TTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRG---EPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLA 1814
            K  +++K  +P        FT+  G+FFPS TG+++G N SGDL +P  +IP G++AA        +Y+   +   A      L   +       +I +K++     + L G ++S++ + L +  GAPR+LQ I+QDNV+P ++   +  +RG   EP  A      I+ L +LI +++++AP++TM FL+ Y  ++ A
Sbjct:  192 KTENMEKNAAPQFGAKQNFFTVF-GVFFPSATGVLSGINMSGDLKDPSSNIPAGTLAALGFRWVGLLYILFAVLLGAVCTREALLTDY-------MIASKVSLVGV-LFLFGLYVSSLSSCLGAQYGAPRVLQVISQDNVVPIIK--PLGKERGANKEPYVASIFVAVIAMLFILIGNLNALAPIVTMPFLVTYASIDYA 380

HSP 2 Score: 80.8777 bits (198), Expect = 1.994e-15
Identity = 55/177 (31.07%), Postives = 86/177 (48.59%), Query Frame = 3

Query:  396 LGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF 926
             GT  GV+  C+ NIFGV+ FLR  W++G +G  L  +I+ +     +  VLS   +     V +GG YF++S  LG   GG + +L+ FG  V+ +++  G  E          SL      D        RV G++    ++  V  G+K+V K   + LA ++ S+L   IG F
Sbjct:   16 FGTWDGVFTSCMLNIFGVIIFLRTGWVVGNAGVGLSLLIIVITLLVALAPVLSSIGVCERCHVGSGGVYFLLSHVLGQRAGGAISLLYAFGQAVSVSLFCAGLGE----------SLAQTAHWDSAWA---VRVIGLLTALAILLVVLAGVKWVVKLQLLLLAILMLSVLDFVIGTF 179

[Close]

BLAST of slc12a-4 vs. Ensembl Medaka
Match: slc12a5a (solute carrier family 12 member 5 [Source:NCBI gene;Acc:101165388])

HSP 1 Score: 916.761 bits (2368), Expect = 0.000e+0
Identity = 498/1167 (42.67%), Postives = 696/1167 (59.64%), Query Frame = 3

Query:  177 ENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGE---------TKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIE-----LDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSL-RVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYIS------FTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFS----------PGA--MHGLK--FDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            +NI KE++P  +     ++  +        L+EEE  T P ++++ + LA Y+   + +            + + K   + +LGT++GVY PC+QNIFGV+ FLR+ W++G+ G    F+IVF+CC +TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VGI FY GTT A AMYI+GAIE++L YIVPKA++F P+E       E  + NN RVYG ILL  M + VF+G+K+V+K + + LACV+ SIL VY G+           VC+LG+R L  S   D  A         +   +++++C   +  LN     YF  NNVT+   IPG    +L +  F  YY  KG L      E   D     + S+ Y+       FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+IAA  TTS VY+S+VI F A +E  VLRDKFG  V   L++  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+D ++P L +F      GEP  +  LT  I   G+LIAS+DS+AP+++MFFLMCY FVNLA AL   L+ P+WRPRFK+YHW  S LG+ LC+ LMF+ SWYYA+VA+ +AG IYKYIE+ GA KEWGD  RG  +SAA+ A+++L + P HTKNWRPQ+L+L++ D     +  P+L+   +QLKAG+GLTIVGT +EG   +    +  A+++L + +  +++KG  ++ V+SN+ +A   L Q SGLG L+HN V+VS+P  W     H  W+  +  +R    + LA+LVPK I  FP    +               G +D+W IVHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LRI+A VEVVE+  +DISAY +++T+ +  R ++L+ +NL   + +RE Q+I D    +                            +    P   T +  + E+      +E     + +L   NT      TP S           A P   L    G  +P +  + T++          PGA     +K  F+  P     N+     RRMH+A+RLNE+I  +SS A L+L+N+PG  KN + D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   20 DNIPKESSPFINSSDAAAEKSQQYDGKNMALFEEEMDTSPMVSSLLSSLANYSNLPQGSKEHEEAENNEAESARKKPVKAPQLGTLMGVYLPCIQNIFGVILFLRMTWLVGIGGVIGTFIIVFMCCTTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFYLGTTYAGAMYILGAIELLLIYIVPKAAIF-PLEGLEGAEAEAALLNNMRVYGTILLTSMATVVFVGVKYVNKLALVFLACVILSILAVYAGVIKTGIEPPFFPVCLLGNRTL-VSKSFDVCAKTLETANGTVTTQMWRIFCD--SPLLNATCDPYFAANNVTQIQGIPGITSGILTENLFGTYYE-KGDLIARTNMESVEDPDDPLTNSNRYVLADITSFFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPVGTIAAITTTSAVYMSSVILFGACIEGVVLRDKFGEGVHGNLVIGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDGLVPALRIFGHGKANGEPTWSLLLTACICESGILIASLDSVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSFLGMSLCLTLMFLCSWYYAIVAMVIAGSIYKYIEFAGAEKEWGDGIRGLSLSAARYALMRLEEGPPHTKNWRPQLLVLVSTDAE-QNVEQPRLLSLTNQLKAGKGLTIVGTALEGTYLENYEQAQRAEQSLRKLMETEKVKGFCQVTVSSNLRDATSHLLQASGLGGLKHNAVLVSWPRNWKQGDDHQNWRNLIELVRETTTARLALLVPKNISAFPSNSERFSE------------GHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQLDDNSIQMKKDLTTFLYHLRIDAMVEVVEMHDSDISAYTYEKTLVMEQRSQMLKQINLTKTEREREIQSITDSSRGS----------------------------IRRKNPAAVTTQLSVTEEPAATSKEEKPEEEV-QLIHDNT------TPASP----------ATPATLLTPGEGAQSPGEQIQMTWTEKCDGEPAKPPGATTTENIKDIFNMKPEWESLNQSNV--RRMHTALRLNEVILKKSSEARLVLLNMPGAPKNRAGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS 1121

[Close]

Query:  177 ENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGE---------TKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIE-----LDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSL-RVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYIS------FTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFS----------PGA--MHGLK--FDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            +NI KE++P  +     ++  +        L+EEE  T P ++++ + LA Y+   + +            + + K   + +LGT++GVY PC+QNIFGV+ FLR+ W++G+ G    F+IVF+CC +TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VGI FY GTT A AMYI+GAIE++L YIVPKA++F P+E       E  + NN RVYG ILL  M + VF+G+K+V+K + + LACV+ SIL VY G+           VC+LG+R L  S   D  A         +   +++++C   +  LN     YF  NNVT+   IPG    +L +  F  YY  KG L      E   D     + S+ Y+       FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+IAA  TTS VY+S+VI F A +E  VLRDKFG  V   L++  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+D ++P L +F      GEP  +  LT  I   G+LIAS+DS+AP+++MFFLMCY FVNLA AL   L+ P+WRPRFK+YHW  S LG+ LC+ LMF+ SWYYA+VA+ +AG IYKYIE+ GA KEWGD  RG  +SAA+ A+++L + P HTKNWRPQ+L+L++ D     +  P+L+   +QLKAG+GLTIVGT +EG   +    +  A+++L + +  +++KG  ++ V+SN+ +A   L Q SGLG L+HN V+VS+P  W     H  W+  +  +R    + LA+LVPK I  FP    +               G +D+W IVHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LRI+A VEVVE+  +DISAY +++T+ +  R ++L+ +NL   + +RE Q+I D    +                            +    P   T +  + E+      +E     + +L   NT      TP S           A P   L    G  +P +  + T++          PGA     +K  F+  P     N+     RRMH+A+RLNE+I  +SS A L+L+N+PG  KN + D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   55 DNIPKESSPFINSSDAAAEKSQQYDGKNMALFEEEMDTSPMVSSLLSSLANYSNLPQGSKEHEEAENNEAESARKKPVKAPQLGTLMGVYLPCIQNIFGVILFLRMTWLVGIGGVIGTFIIVFMCCTTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFYLGTTYAGAMYILGAIELLLIYIVPKAAIF-PLEGLEGAEAEAALLNNMRVYGTILLTSMATVVFVGVKYVNKLALVFLACVILSILAVYAGVIKTGIEPPFFPVCLLGNRTL-VSKSFDVCAKTLETANGTVTTQMWRIFC--DSPLLNATCDPYFAANNVTQIQGIPGITSGILTENLFGTYYE-KGDLIARTNMESVEDPDDPLTNSNRYVLADITSFFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPVGTIAAITTTSAVYMSSVILFGACIEGVVLRDKFGEGVHGNLVIGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDGLVPALRIFGHGKANGEPTWSLLLTACICESGILIASLDSVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSFLGMSLCLTLMFLCSWYYAIVAMVIAGSIYKYIEFAGAEKEWGDGIRGLSLSAARYALMRLEEGPPHTKNWRPQLLVLVSTDAE-QNVEQPRLLSLTNQLKAGKGLTIVGTALEGTYLENYEQAQRAEQSLRKLMETEKVKGFCQVTVSSNLRDATSHLLQASGLGGLKHNAVLVSWPRNWKQGDDHQNWRNLIELVRETTTARLALLVPKNISAFPSNSERFSE------------GHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQLDDNSIQMKKDLTTFLYHLRIDAMVEVVEMHDSDISAYTYEKTLVMEQRSQMLKQINLTKTEREREIQSITDSSRGS----------------------------IRRKNPAAVTTQLSVTEEPAATSKEEKPEEEV-QLIHDNT------TPASP----------ATPATLLTPGEGAQSPGEQIQMTWTEKCDGEPAKPPGATTTENIKDIFNMKPEWESLNQSNV--RRMHTALRLNEVILKKSSEARLVLLNMPGAPKNRAGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS 1156

[Close]

BLAST of slc12a-4 vs. Ensembl Medaka
Match: slc12a5a (solute carrier family 12 member 5 [Source:NCBI gene;Acc:101165388])

HSP 1 Score: 914.45 bits (2362), Expect = 0.000e+0
Identity = 497/1165 (42.66%), Postives = 694/1165 (59.57%), Query Frame = 3

Query:  177 ENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGE---------TKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIE-----LDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSL-RVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYIS------FTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFS----------PGA--MHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            +NI KE++P  +     ++  +        L+EEE  T P ++++ + LA Y+   + +            + + K   + +LGT++GVY PC+QNIFGV+ FLR+ W++G+ G    F+IVF+CC +TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VGI FY GTT A AMYI+GAIE++L YIVPKA++F P+E       E  + NN RVYG ILL  M + VF+G+K+V+K + + LACV+ SIL VY G+           VC+LG+R L  S   D  A         +   +++++C   +  LN     YF  NNVT+   IPG    +L +  F  YY  KG L      E   D     + S+ Y+       FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+IAA  TTS VY+S+VI F A +E  VLRDKFG  V   L++  LAWP+ WVI+IGSF ST GAGLQSLTGAPRLLQAIA+D ++P L +F      GEP  +  LT  I   G+LIAS+DS+AP+++MFFLMCY FVNLA AL   L+ P+WRPRFK+YHW  S LG+ LC+ LMF+ SWYYA+VA+ +AG IYKYIE+ GA KEWGD  RG  +SAA+ A+++L + P HTKNWRPQ+L+L++ D     +  P+L+   +QLKAG+GLTIVGT +EG   +    +  A+++L + +  +++KG  ++ V+SN+ +A   L Q SGLG L+HN V+VS+P  W     H  W+  +  +R    + LA+LVPK I  FP    +               G +D+W IVHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LRI+A VEVVE+  +DISAY +++T+ +  R ++L+ +NL   + +RE Q+I D    +                            +    P   T +  + E+      +E     + +L   NT      TP S           A P   L    G  +P +  + T++          PGA     +K  F+      N      RRMH+A+RLNE+I  +SS A L+L+N+PG  KN + D NYM+++E LT+GL RVL VRG G+EVIT ++
Sbjct:   55 DNIPKESSPFINSSDAAAEKSQQYDGKNMALFEEEMDTSPMVSSLLSSLANYSNLPQGSKEHEEAENNEAESARKKPVKAPQLGTLMGVYLPCIQNIFGVILFLRMTWLVGIGGVIGTFIIVFMCCTTTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGICFYLGTTYAGAMYILGAIELLLIYIVPKAAIF-PLEGLEGAEAEAALLNNMRVYGTILLTSMATVVFVGVKYVNKLALVFLACVILSILAVYAGVIKTGIEPPFFPVCLLGNRTL-VSKSFDVCAKTLETANGTVTTQMWRIFC--DSPLLNATCDPYFAANNVTQIQGIPGITSGILTENLFGTYYE-KGDLIARTNMESVEDPDDPLTNSNRYVLADITSFFTLLVGIYFPSVTGIMAGSNRSGDLRDAQKSIPVGTIAAITTTSAVYMSSVILFGACIEGVVLRDKFGEGVHGNLVIGTLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDGLVPALRIFGHGKANGEPTWSLLLTACICESGILIASLDSVAPILSMFFLMCYMFVNLACALQTLLRTPNWRPRFKFYHWALSFLGMSLCLTLMFLCSWYYAIVAMVIAGSIYKYIEFAGAEKEWGDGIRGLSLSAARYALMRLEEGPPHTKNWRPQLLVLVSTDAE-QNVEQPRLLSLTNQLKAGKGLTIVGTALEGTYLENYEQAQRAEQSLRKLMETEKVKGFCQVTVSSNLRDATSHLLQASGLGGLKHNAVLVSWPRNWKQGDDHQNWRNLIELVRETTTARLALLVPKNISAFPSNSERFSE------------GHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQLDDNSIQMKKDLTTFLYHLRIDAMVEVVEMHDSDISAYTYEKTLVMEQRSQMLKQINLTKTEREREIQSITDSSRGS----------------------------IRRKNPAAVTTQLSVTEEPAATSKEEKPEEEV-QLIHDNT------TPASP----------ATPATLLTPGEGAQSPGEQIQMTWTEKCDGEPAKPPGATTTENIKDIFNMKPNQSNV-----RRMHTALRLNEVILKKSSEARLVLLNMPGAPKNRAGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS 1151

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BLAST of slc12a-4 vs. Ensembl Medaka
Match: slc12a7b (solute carrier family 12 member 7 [Source:NCBI gene;Acc:101159301])

HSP 1 Score: 902.508 bits (2331), Expect = 0.000e+0
Identity = 501/1193 (41.99%), Postives = 713/1193 (59.77%), Query Frame = 3

Query:   78 HRTSEVINSENDLSSVAAEKAAHYVVESKVENSENITKEATPL---TDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGP--IELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF--VANPSRSLRVCMLGDRLLNRSVVLDKEANNTF-RCTKD-------INGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEP------GKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
             + +E    E+D+S +  E A            + + KE +P    TD +   S D      +   L+EEE  + P +A++ NKLA YT          E    E  K K   S ++GT +GVY PCLQNI GV+ FLRL WI+G +G     VIV +CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ F+ GTT A +MYI+G IE++L YIVP A++F     E +   + NN RVYG   L +M   VF+G+K+V+K + + LACV+ SI+ +Y G+   +  P     VCMLG+R L          NN F +C K        +   L+KL+C   + +LN    +YF  NNVT    IPG    ++ +  +  Y   +G L          G +P  D      V+DI   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTS +Y+S VI F A +E  +LRDKFG SV   L++  L+WP+ WVI+IGSF S  GAGLQSLTGAPRLLQAIA+D ++PFL+VF      GEP  A  LT  I  +G+LIAS+DS+AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHWT S LG+ LC+ALMF+SSWYYA++A+ +AG IYKYIEYRGA KEWGD  RG  ++AA+ A+++L + P HTKNWRPQ+L+L+NLD     + HP+L+   +QLKAG+GLTIVG V+EG    K++++  A++ +  ++  ++ KG   +VV+SN+ + +  L Q++GLG ++HN V++++P  W        W+ F+ T+R   A+ LA+LV K +D FP  + +               GT+D+W +VHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LR+NA+VEVVE+   DISA+ +++T+ +  R ++L+ M+L   + +RE Q+I D+                       S +   N D  E     +   T   ED   DE +   + +++   ++N +       ++ P +   + T      + N  R   A   + +            F+  P     N+     RRMH+AV+LNE++ ++S  A L+L+N+PGP KN   D NYM+++E L +GL RVL VRG G+EVIT ++
Sbjct:   15 RKAAEWNEEEDDISELKEEDAT----------GDGVPKENSPFINSTDHDKNNSYD-----GTNMALFEEEMDSNPMVASLLNKLANYTNLSQGAQEHEEADEDEGPKKKAVKSPQMGTFMGVYLPCLQNILGVILFLRLTWIVGTAGILESLVIVGLCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFFLGTTFAGSMYILGTIEILLTYIVPAAAIFKAEKKEDEAGALLNNMRVYGTCCLTLMAVVVFVGVKYVNKLALVFLACVILSIIAIYAGVIKTIFEPP-DFPVCMLGNRTLK---------NNDFDKCLKTDIIDNLTVTTALWKLFC--DSPELNATCNEYFSNNNVTLMQGIPGLTSGVISENMWSEY-GPQGMLVENRRVPSFGGSDPSQDIYMPYVVNDITSFFTLLVGIYFPSVTGIMAGSNRSGDLRDAQRSIPIGTILAITTTSIIYVSCVILFGACIEGVLLRDKFGDSVKGNLVIGTLSWPSPWVIVIGSFFSCCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTAAICEIGILIASLDSVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFISSWYYAIIAMVIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALIRLEESPPHTKNWRPQMLVLLNLDSD-QAVKHPRLLSLTTQLKAGKGLTIVGYVLEGTYLTKETEAKKAEQNIKSSMSAERTKGFCHVVVSSNLRDGVSHLIQSAGLGGMKHNTVLMAWPGTWKQSNDPQSWRNFIETIRETTAAHLALLVAKNVDSFPTNQERLGE------------GTIDVWWVVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLQMFLYHLRLNAEVEVVEMHDNDISAFTYEKTLVMEQRSQMLKQMHLSRTEREREIQSITDESR---------------------SSIRRKNQDATEGSSLSRQSSTA--EDTQEDEAQLIHDRNMASHAAANDK------AEAGPERVHMTWTKDKLFTERNRNRECNANVAVRDL-----------FNMKPEWESLNQSNV--RRMHTAVKLNEVVVNKSQGAHLVLLNMPGPPKNRGGDENYMEFLEVLLEGLNRVLLVRGGGREVITIYS 1124

[Close]

BLAST of slc12a-4 vs. Ensembl Medaka
Match: slc12a7b (solute carrier family 12 member 7 [Source:NCBI gene;Acc:101159301])

HSP 1 Score: 899.042 bits (2322), Expect = 0.000e+0
Identity = 495/1160 (42.67%), Postives = 702/1160 (60.52%), Query Frame = 3

Query:  177 ENITKEATPL---TDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTG-------GIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGP--IELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIF--VANPSRSLRVCMLGDRLLNRSVVLDKEANNTF-RCTKD-------INGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEP------GKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            + + KE +P    TD +   S D      +   L+EEE  + P +A++ NKLA YT          E    E  K K   S ++GT +GVY PCLQNI GV+ FLRL WI+G +G     VIV +CC  TMLT +SMSAIATNG VPAGGSY+MISRSLGPEFGG VG+ F+ GTT A +MYI+G IE++L YIVP A++F     E +   + NN RVYG   L +M   VF+G+K+V+K + + LACV+ SI+ +Y G+   +  P     VCMLG+R L          NN F +C K        +   L+KL+C   + +LN    +YF  NNVT    IPG    ++ +  +  Y   +G L          G +P  D      V+DI   FT+L+GI+FPSVTGIMAGSNRSGDL + Q SIP+G+I A  TTS +Y+S VI F A +E  +LRDKFG SV   L++  L+WP+ WVI+IGSF S  GAGLQSLTGAPRLLQAIA+D ++PFL+VF      GEP  A  LT  I  +G+LIAS+DS+AP+++MFFLMCY FVNLA A+   L+ P+WRPRFKYYHWT S LG+ LC+ALMF+SSWYYA++A+ +AG IYKYIEYRGA KEWGD  RG  ++AA+ A+++L + P HTKNWRPQ+L+L+NLD     + HP+L+   +QLKAG+GLTIVG V+EG    K++++  A++ +  ++  ++ KG   +VV+SN+ + +  L Q++GLG ++HN V++++P  W        W+ F+ T+R   A+ LA+LV K +D FP  + +               GT+D+W +VHDGG+L+LL  LL + + W KC++R+F VA   DN+I +KK++  F+Y LR+NA+VEVVE+   DISA+ +++T+ +  R ++L+ M+L   + +RE Q+I D+                       S +   N D  E     +   T   ED   DE +   + +++   ++N +       ++ P +   + T      + N  R   A   + +            F+  P     N+     RRMH+AV+LNE++ ++S  A L+L+N+PGP KN   D NYM+++E L +GL RVL VRG G+EVIT ++
Sbjct:  123 DGVPKENSPFINSTDHDKNNSYD-----GTNMALFEEEMDSNPMVASLLNKLANYTNLSQGAQEHEEADEDEGPKKKAVKSPQMGTFMGVYLPCLQNILGVILFLRLTWIVGTAGILESLVIVGLCCSCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFFLGTTFAGSMYILGTIEILLTYIVPAAAIFKAEKKEDEAGALLNNMRVYGTCCLTLMAVVVFVGVKYVNKLALVFLACVILSIIAIYAGVIKTIFEPP-DFPVCMLGNRTLK---------NNDFDKCLKTDIIDNLTVTTALWKLFC--DSPELNATCNEYFSNNNVTLMQGIPGLTSGVISENMWSEY-GPQGMLVENRRVPSFGGSDPSQDIYMPYVVNDITSFFTLLVGIYFPSVTGIMAGSNRSGDLRDAQRSIPIGTILAITTTSIIYVSCVILFGACIEGVLLRDKFGDSVKGNLVIGTLSWPSPWVIVIGSFFSCCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTAAICEIGILIASLDSVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKYYHWTLSFLGMSLCLALMFISSWYYAIIAMVIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALIRLEESPPHTKNWRPQMLVLLNLDSD-QAVKHPRLLSLTTQLKAGKGLTIVGYVLEGTYLTKETEAKKAEQNIKSSMSAERTKGFCHVVVSSNLRDGVSHLIQSAGLGGMKHNTVLMAWPGTWKQSNDPQSWRNFIETIRETTAAHLALLVAKNVDSFPTNQERLGE------------GTIDVWWVVHDGGMLMLLPFLLRQHKVWRKCKMRIFTVAQMDDNSIQMKKDLQMFLYHLRLNAEVEVVEMHDNDISAFTYEKTLVMEQRSQMLKQMHLSRTEREREIQSITDESR---------------------SSIRRKNQDATEGSSLSRQSSTA--EDTQEDEAQLIHDRNMASHAAANDKA------EAGPERVHMTWTKDKLFTERNRNRECNANVAVRDL-----------FNMKPEWESLNQSNV--RRMHTAVKLNEVVVNKSQGAHLVLLNMPGPPKNRGGDENYMEFLEVLLEGLNRVLLVRGGGREVITIYS 1209

[Close]

BLAST of slc12a-4 vs. Planmine SMEST
Match: SMESG000019076.1 (SMESG000019076.1)

HSP 1 Score: 2385.91 bits (6182), Expect = 0.000e+0
Identity = 1164/1174 (99.15%), Postives = 1165/1174 (99.23%), Query Frame = 3

Query:   54 MNEARFVVHRTSEVINSENDLSSVAAEKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE 3575
            MNEARFVVHRTSEVINSENDL+SVAAEKAAHY         ENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGLLLLLAHLLIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE
Sbjct:    1 MNEARFVVHRTSEVINSENDLNSVAAEKAAHY--------GENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGLLLLLAHLLIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE 1166

[Close]

BLAST of slc12a-4 vs. Planmine SMEST
Match: SMESG000019076.1 (SMESG000019076.1)

HSP 1 Score: 2377.05 bits (6159), Expect = 0.000e+0
Identity = 1161/1174 (98.89%), Postives = 1162/1174 (98.98%), Query Frame = 3

Query:   54 MNEARFVVHRTSEVINSENDLSSVAAEKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE 3575
            MNEARFVVHRTSEVINSENDL+SVAAEKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGLLLLLAHLLIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLK            QTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE
Sbjct:    1 MNEARFVVHRTSEVINSENDLNSVAAEKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGLLLLLAHLLIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPTEKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQRSVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLK------------QTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFAE 1162

[Close]

BLAST of slc12a-4 vs. Planmine SMEST
Match: SMESG000043221.1 (SMESG000043221.1)

HSP 1 Score: 342.043 bits (876), Expect = 1.364e-105
Identity = 201/474 (42.41%), Postives = 271/474 (57.17%), Query Frame = 3

Query:   93 VINSENDLSSVAAEKAAHYVVESKVENSENITKEATPLTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLF----GPIELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVG 1502
            ++N EN   SV+       + + K+   E + K ++ LTD   E  + ++   D    LY+E++       +I N ++ Y   +   + +T    +K + KL  I+GVY P LQ+IFGV+ FLRL WIIG SG   GF +VF+CC  T LT +SMSAIATNGKV +GGSYFMISR+LGP+FG  +GILFY G  +A AMY++G IE++L Y+ P    F    G    D   MFNN R Y   LL ++   V +G+KFV  F+ I+LACV+ SI+CVY+G FV +P RS  VC LG+RLL +S       N T  C K +   L   YC        D+   YFK N V   PA+PG+   ++K   +  Y +    + G  G       G      D+  +F +L+ I+FPSVTGIM GSN SGDL +PQ  IP  +IAA  TTSFVYLS V  F+ T    VL DK+G S+G
Sbjct:   28 LLNDENREHSVSFRDRHSIINDQKIGKLE-VRKVSSGLTDNVEEGHESDDDVKDETMALYQEDY-------SIMNSISGY---LRAMTYQTSVAGQKKNPKLNGIIGVYLPTLQHIFGVIMFLRLYWIIGNSGFLQGFFMVFICCLCTFLTSISMSAIATNGKVKSGGSYFMISRNLGPQFGSAIGILFYLGNAIATAMYLVGTIEILLTYVAPTLPQFQSKPGSSHDDHTAMFNNFRTYASGLLFLVFVIVALGVKFVQLFAPITLACVIISIVCVYLGGFVRSPERSEMVCFLGERLLTKSF---WTINKTLSCNKTM---LEDYYCNGNLTTFCDD---YFKANPVQYLPAMPGYASGVIKSNLWPVYMSEGEVMPGVKG------LGGRDVTRDMMTNFFVLLAIYFPSVTGIMTGSNMSGDLKDPQKDIPRYTIAAQLTTSFVYLSFVFIFAGTTRREVLLDKYGVSMG 475

[Close]

BLAST of slc12a-4 vs. Planmine SMEST
Match: SMESG000043220.1 (SMESG000043220.1)

HSP 1 Score: 317.775 bits (813), Expect = 2.088e-96
Identity = 167/388 (43.04%), Postives = 243/388 (62.63%), Query Frame = 3

Query: 1788 MCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLD-ETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDK--KSDSIIAKETLNRAIRDKQIKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWA--NDKSHIKWKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGTVDMWCIVHDGGXXXXXXXXXIRARAWGKCELRVFVVASEKDNTITLKKEVIRFVYDLRINAQVEVVELDQAD-ISAYAFQRTMDLNMRKKILEDMNLRTVDTKREFQAILD 2933
            M Y FVNLA AL   L  P+WRPRF+YYHW  SL+G  L I ++F + WYY LV +AL   IYKY+EYRGA KEWGD  RG +++ AK ++L + ++  HTKNWRPQILIL   + + C K  +PKLI F  QLK GRGL I+ +++EG+  DK  +  +   KE L   +   ++KG  + +V S+V E I  L Q+ GLG L+ N VMV +P  W   +D+   +  +F+  +    ++++ +L+PKG+ ++P                +   GT+D+W I+ DGGLL++L  LL R R W +CE+RVF VA++ DN+I +K  +++++YDLRI A VEVVEL  +D ISAY  Q+T+ +  R+K++E M L   +   E QA +D
Sbjct:    1 MLYAFVNLACALQNILNAPNWRPRFRYYHWFLSLVGAALSIFIIFATYWYYGLVTVALTLAIYKYVEYRGAEKEWGDGIRGLKLAGAKYSLLNI-EEETHTKNWRPQILILCKTNSDLCIK--YPKLISFAGQLKGGRGLVILASLVEGDYADKDVRDKAQKVKENLKECMATTKVKGFVQTIVCSDVKEGISTLIQSIGLGALKPNTVMVGWPHTWKGISDEESPE-HEFLDAIHRVVSAEICMLIPKGVSEYP---------------SISCKGTIDIWWIMQDGGLLMMLPVLLKRHRVWERCEVRVFTVATQSDNSILMKDSLVKWLYDLRIKATVEVVELPNSDAISAYLHQKTLVMEEREKLIEQMKLTAKEVDLEPQARID 369

HSP 2 Score: 89.3521 bits (220), Expect = 4.964e-18
Identity = 44/105 (41.90%), Postives = 67/105 (63.81%), Query Frame = 3

Query: 3273 NEFTFSPGAMHGLK-----FDFSPSLGHKNRQTKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVETLTDGLARVLFVRGTGKEVITAFA 3572
            ++FTFSP  +  +K        +      N + +  ++MH+AV LN +I+ +S NA L+++NLPGP KN+ S   Y+ Y+  L++GL RVL VRGTGKEVIT ++
Sbjct:  390 HQFTFSPATLAQMKDVLEMKTTTKKSKKLNPENEEAKKMHNAVELNILIKEKSLNADLVILNLPGPPKNKHSLECYLNYLGVLSEGLPRVLLVRGTGKEVITIYS 494

[Close]

BLAST of slc12a-4 vs. Planmine SMEST
Match: SMESG000033021.1 (SMESG000033021.1)

HSP 1 Score: 256.914 bits (655), Expect = 1.710e-70
Identity = 194/703 (27.60%), Postives = 335/703 (47.65%), Query Frame = 3

Query:  276 EEFHTRPPMANIFNKLAQYTGGIEPTSGETKKLK-EKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALGFVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGILFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPI-ELDENIMFNNTRVYGVILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLRVCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEACKYFKENNVTEFPAIPGFYRPLLKDGFFQNY---YTTKGSLYGEPGKEPHLDKGQSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAACATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEW--VILIGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIP-FLEVFKVTTKRGEPLRAQALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWRPRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGATKEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMSHPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRD-KQIKGLSEIVVASNVIEAIK 2357
            E   T P +   + + ++Y    +PT  ET  L  +++   L T  GV+ P     F  L FLRL +++G +G  L  + + +     +LTVLS+ AI+TNG +  GG YFMISR+LGPE GG++GILF+     ++A+++   +E ++         FGP+  +D +++  N     +   C+ + C+ + +   S F+  +L   +   +C +                        SV++      +F   K    P+ K+                +  +N T+F    G       +  + NY   +TTK                        Y++F  +  I FP VTGIM G+N SG+L  P  SIP G++AA   T   Y+ +    +AT +  +L + +            +   N W   ++IG FL+ + A L +  GA R+L A+AQD++   FL+  K+TTK G P+ +  ++F +  + +LI  ++ IAP++++FFL+ Y  VNLA AL  +    ++RP FKY+ W TSLLG+  C+ + F++S+ Y+L   +L   I   + +R     WG  ++       +K +L +  +  H K WRPQIL+L++        S   L+ F + LK G GL ++G VI+ +    + + +   E     ++D  +IK   ++ +A  + +  +
Sbjct:    5 EVLRTFPSINEEYAQNSRYYH--DPTIIETLTLSSDQTKRNLKTFTGVFCPVALAQFSSLLFLRLGFVLGQTGLLLAILQLIMAYLILLLTVLSICAISTNGAIKGGGVYFMISRTLGPEVGGSIGILFFLANVFSSALFMAAFVEGLVDD-------FGPLGRVDADLIQENFGWNYLFATCVCIFCLGVCLVGGSLFAKTNLFIFIIVFVCFF------------------------SVIV------SFLFHKAFQVPIPKI------------NTIVYSNSNETQFGNYTGLSGTTFINNLYANYTVDFTTKK-----------------------YMTFFTVFAILFPGVTGIMNGANMSGELIKPAVSIPRGTLAATLFTFICYIISFFLSAATCDRFLLWNNY----------LFMQATNLWKPFVIIGMFLTCISAALGNFIGASRILFALAQDDIFGIFLKPAKLTTKGGNPIGSVVISFVLVQIVLLIGKVNVIAPVVSVFFLLAYASVNLACALLDWAAASNFRPTFKYFSWYTSLLGVACCLGMCFLASYIYSLGIFSLFFIIIVILNFRSLDYSWGSISQALLFHQVRKYLLMIDIRKEHVKYWRPQILLLLS-----NPRSQASLVQFVNILKKG-GLFVIGHVIKTDFEHIEKNPVHEIEGNFAKMKDFLKIKAFIDVTIAPTIRDGCR 617

The following BLAST results are available for this feature:

BLAST of slc12a-4 vs. Ensembl Human
BLAST of slc12a-4 vs. Ensembl Celegans
BLAST of slc12a-4 vs. Ensembl Fly
BLAST of slc12a-4 vs. Ensembl Zebrafish
BLAST of slc12a-4 vs. Ensembl Xenopus
BLAST of slc12a-4 vs. Ensembl Mouse
BLAST of slc12a-4 vs. UniProt/SwissProt
BLAST of slc12a-4 vs. TrEMBL
BLAST of slc12a-4 vs. Ensembl Cavefish
BLAST of slc12a-4 vs. Ensembl Sea Lamprey
BLAST of slc12a-4 vs. Ensembl Yeast
BLAST of slc12a-4 vs. Ensembl Nematostella
BLAST of slc12a-4 vs. Ensembl Medaka
BLAST of slc12a-4 vs. Planmine SMEST

BLAST of slc12a-4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
SLC12A5	0.000e+0	42.93	solute carrier family 12 member 5 [Source:HGNC Sym...	[more]
SLC12A5	0.000e+0	43.47	solute carrier family 12 member 5 [Source:HGNC Sym...	[more]
SLC12A6	0.000e+0	43.65	solute carrier family 12 member 6 [Source:HGNC Sym...	[more]
SLC12A6	0.000e+0	43.65	solute carrier family 12 member 6 [Source:HGNC Sym...	[more]
SLC12A6	0.000e+0	43.81	solute carrier family 12 member 6 [Source:HGNC Sym...	[more]

BLAST of slc12a-4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5


Match Name	E-value	Identity	Description
kcc-2	0.000e+0	40.10	K+/Cl-Cotransporter [Source:UniProtKB/TrEMBL;Acc:...	[more]
kcc-2	0.000e+0	40.56	K+/Cl-Cotransporter [Source:UniProtKB/TrEMBL;Acc:...	[more]
kcc-2	0.000e+0	40.07	K+/Cl-Cotransporter [Source:UniProtKB/TrEMBL;Acc:...	[more]
kcc-2	0.000e+0	40.56	K+/Cl-Cotransporter [Source:UniProtKB/TrEMBL;Acc:...	[more]
kcc-2	0.000e+0	40.80	K+/Cl-Cotransporter [Source:UniProtKB/TrEMBL;Acc:...	[more]

BLAST of slc12a-4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
kcc	0.000e+0	43.83	gene:FBgn0261794 transcript:FBtr0072139	[more]
kcc	0.000e+0	43.85	gene:FBgn0261794 transcript:FBtr0343301	[more]
kcc	0.000e+0	44.35	gene:FBgn0261794 transcript:FBtr0072137	[more]
kcc	0.000e+0	44.35	gene:FBgn0261794 transcript:FBtr0343300	[more]
kcc	0.000e+0	44.35	gene:FBgn0261794 transcript:FBtr0343299	[more]

BLAST of slc12a-4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
slc12a5a	0.000e+0	44.63	solute carrier family 12 member 5a [Source:ZFIN;Ac...	[more]
slc12a7a	0.000e+0	42.57	solute carrier family 12 member 7a [Source:ZFIN;Ac...	[more]
slc12a4	0.000e+0	43.64	solute carrier family 12 member 4 [Source:ZFIN;Acc...	[more]
slc12a7a	0.000e+0	42.58	solute carrier family 12 member 7a [Source:ZFIN;Ac...	[more]
slc12a7a	0.000e+0	42.67	solute carrier family 12 member 7a [Source:ZFIN;Ac...	[more]

BLAST of slc12a-4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5


Match Name	E-value	Identity	Description
slc12a5	0.000e+0	44.01	solute carrier family 12 member 5 [Source:NCBI gen...	[more]
slc12a5	0.000e+0	44.01	solute carrier family 12 member 5 [Source:NCBI gen...	[more]
slc12a5	0.000e+0	44.01	solute carrier family 12 member 5 [Source:NCBI gen...	[more]
slc12a5	0.000e+0	43.81	solute carrier family 12 member 5 [Source:NCBI gen...	[more]
slc12a5	0.000e+0	43.27	solute carrier family 12 member 5 [Source:NCBI gen...	[more]

BLAST of slc12a-4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5


Match Name	E-value	Identity	Description
Slc12a5	0.000e+0	43.10	solute carrier family 12, member 5 [Source:MGI Sym...	[more]
Slc12a5	0.000e+0	43.10	solute carrier family 12, member 5 [Source:MGI Sym...	[more]
Slc12a4	0.000e+0	45.45	solute carrier family 12, member 4 [Source:MGI Sym...	[more]
Slc12a5	0.000e+0	43.64	solute carrier family 12, member 5 [Source:MGI Sym...	[more]
Slc12a4	0.000e+0	45.45	solute carrier family 12, member 4 [Source:MGI Sym...	[more]

BLAST of slc12a-4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q91V14\|S12A5_MOUSE	0.000e+0	43.10	Solute carrier family 12 member 5 OS=Mus musculus ...	[more]
sp\|Q63633\|S12A5_RAT	0.000e+0	43.02	Solute carrier family 12 member 5 OS=Rattus norveg...	[more]
sp\|Q9H2X9\|S12A5_HUMAN	0.000e+0	42.93	Solute carrier family 12 member 5 OS=Homo sapiens ...	[more]
sp\|Q63632\|S12A4_RAT	0.000e+0	45.49	Solute carrier family 12 member 4 OS=Rattus norveg...	[more]
sp\|Q9JIS8\|S12A4_MOUSE	0.000e+0	45.45	Solute carrier family 12 member 4 OS=Mus musculus ...	[more]

BLAST of slc12a-4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0H3YK51	0.000e+0	98.94	Slc12a-4 OS=Schmidtea mediterranea OX=79327 GN=slc...	[more]
A0A5K4F357	0.000e+0	54.06	Uncharacterized protein OS=Schistosoma mansoni OX=...	[more]
A0A094ZXI2	0.000e+0	53.56	Solute carrier family 12 member 5 OS=Schistosoma h...	[more]
A0A4Z2DI78	0.000e+0	54.02	Solute carrier family 12 member 5 OS=Schistosoma j...	[more]
A0A4V3SEW3	0.000e+0	52.86	Uncharacterized protein OS=Opisthorchis felineus O...	[more]

BLAST of slc12a-4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
slc12a5a	0.000e+0	43.55	solute carrier family 12 member 5 [Source:NCBI gen...	[more]
slc12a7b	0.000e+0	43.04	solute carrier family 12 member 7-like [Source:NCB...	[more]
slc12a5a	0.000e+0	43.43	solute carrier family 12 member 5 [Source:NCBI gen...	[more]
slc12a7b	0.000e+0	43.37	solute carrier family 12 member 7-like [Source:NCB...	[more]
slc12a5a	0.000e+0	43.46	solute carrier family 12 member 5 [Source:NCBI gen...	[more]

BLAST of slc12a-4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5


Match Name	E-value	Identity	Description
slc12a4	0.000e+0	46.85	solute carrier family 12 member 4 [Source:ZFIN;Acc...	[more]
slc12a4	0.000e+0	46.85	solute carrier family 12 member 4 [Source:ZFIN;Acc...	[more]
slc12a9	9.230e-74	28.17	solute carrier family 12 member 9 [Source:NCBI gen...	[more]
ENSPMAT00000000732.1	1.312e-33	27.40	pep scaffold:Pmarinus_7.0:GL479336:3629:33813:1 ge...	[more]
ENSPMAT00000003053.1	1.278e-32	25.64	pep scaffold:Pmarinus_7.0:GL478333:5880:33924:-1 g...	[more]

BLAST of slc12a-4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1


Match Name	E-value	Identity	Description
VHC1	3.409e-43	26.61	Vacuolar membrane cation-chloride cotransporter (C...	[more]

BLAST of slc12a-4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5


Match Name	E-value	Identity	Description
EDO35443	0.000e+0	40.33	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41391	1.882e-56	32.11	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO49361	2.350e-56	27.66	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO41917	2.272e-51	32.09	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO46430	8.146e-20	31.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of slc12a-4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5


Match Name	E-value	Identity	Description
slc12a5a	0.000e+0	42.67	solute carrier family 12 member 5 [Source:NCBI gen...	[more]
slc12a5a	0.000e+0	42.67	solute carrier family 12 member 5 [Source:NCBI gen...	[more]
slc12a5a	0.000e+0	42.66	solute carrier family 12 member 5 [Source:NCBI gen...	[more]
slc12a7b	0.000e+0	41.99	solute carrier family 12 member 7 [Source:NCBI gen...	[more]
slc12a7b	0.000e+0	42.67	solute carrier family 12 member 7 [Source:NCBI gen...	[more]

BLAST of slc12a-4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000019076.1	0.000e+0	99.15	SMESG000019076.1	[more]
SMESG000019076.1	0.000e+0	98.89	SMESG000019076.1	[more]
SMESG000043221.1	1.364e-105	42.41	SMESG000043221.1	[more]
SMESG000043220.1	2.088e-96	43.04	SMESG000043220.1	[more]
SMESG000033021.1	1.710e-70	27.60	SMESG000033021.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Cross References

External references for this transcript


Database	Accession
SmedGD GBrowse	SMED30003346
GenBank	AKN21478.1

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30003346 ID=SMED30003346|Name=slc12a-4|organism=Schmidtea mediterranea sexual|type=transcript|length=3681bp
CAAAATTTAAAATTTCAATTTTAATGAAATATTTTGATAACTTATAATTT
TAAATGAATGAAGCAAGATTCGTTGTTCACCGGACAAGCGAAGTAATAAA
TTCAGAAAATGATCTGAGTTCTGTTGCTGCTGAAAAAGCAGCTCATTATG
TAGTGGAGTCAAAAGTCGAAAATAGCGAGAACATAACAAAAGAAGCAACT
CCTTTGACAGATGAGGAAATAGAAATGAGTGATGATGAAGAACCAGCAGG
AGACAGCAAATTTGACCTTTACGAGGAAGAATTTCACACCCGACCTCCGA
TGGCTAATATATTCAATAAGCTCGCCCAATACACTGGTGGAATAGAGCCC
ACGTCTGGGGAAACTAAAAAGCTAAAAGAAAAATCGTCGAATAAATTGGG
AACAATTCTCGGTGTTTATTTTCCATGCTTACAAAATATATTTGGAGTTT
TATTTTTCTTGCGGTTGGTATGGATTATAGGAGTTTCAGGAACCGCTTTG
GGATTTGTCATAGTATTCGTCTGCTGTTGTTCGACAATGCTCACAGTTTT
AAGTATGTCTGCAATAGCAACTAATGGCAAAGTACCTGCTGGTGGCTCAT
ATTTTATGATTAGCAGATCTCTCGGTCCAGAATTCGGTGGAACAGTCGGT
ATCCTTTTCTATTTTGGTACCACTGTGGCCGCGGCTATGTATATCATCGG
TGCAATAGAAGTTATGCTTAAATATATTGTTCCCAAAGCAAGTTTATTCG
GTCCAATTGAACTCGACGAAAATATTATGTTTAACAATACAAGGGTTTAT
GGGGTCATCTTACTCTGCATCATGATGAGTTGCGTATTTATCGGGATTAA
ATTCGTCAGTAAATTCTCAACTATTAGTTTAGCTTGTGTTCTCTGCTCAA
TATTATGTGTATATATTGGTATATTTGTTGCCAATCCTTCAAGATCACTT
AGAGTATGCATGCTAGGTGATCGTTTACTAAATAGAAGTGTTGTTTTGGA
TAAAGAAGCAAACAATACCTTCAGATGTACCAAAGATATAAATGGACCTT
TATATAAATTATATTGTCGAAATGGAACCAAAGACCTAAATGATGAGGCT
TGTAAATATTTTAAAGAAAACAATGTGACGGAGTTTCCTGCTATTCCTGG
ATTTTATAGACCACTATTAAAAGATGGATTTTTTCAAAATTATTACACAA
CCAAAGGTAGTCTCTATGGTGAACCGGGAAAAGAGCCCCATTTAGATAAA
GGCCAATCACCCAGTGTTTCTGATATCTATATTTCTTTCACAATTTTAAT
AGGAATATTTTTCCCTTCCGTTACAGGTATTATGGCTGGTTCCAATAGGT
CTGGAGATTTAGCCAATCCACAATTTTCAATTCCTCTTGGATCTATTGCT
GCTTGTGCTACTACCTCATTTGTCTATTTATCTGCTGTCATATTTTTCTC
TGCAACAGTTGAACATACTGTTTTAAGAGATAAATTCGGGGGAAGTGTCG
GTGAAGGGTTGATAGTTGCTAAATTAGCCTGGCCTAACGAATGGGTCATT
CTTATTGGCTCATTCCTTAGCACTGTTGGAGCTGGATTGCAGTCCTTAAC
CGGAGCTCCGAGATTGTTACAAGCAATCGCACAAGACAATGTCATACCGT
TTTTGGAAGTCTTTAAAGTTACTACAAAACGTGGAGAACCATTGCGAGCT
CAGGCTTTAACATTTTTTATTTCTTTCCTAGGAGTTCTCATTGCCAGTAT
TGATTCAATTGCACCATTAATTACCATGTTTTTTTTAATGTGTTACGGAT
TTGTGAATCTCGCTACTGCATTAAACGGATTTCTCCAGGAACCCAGTTGG
AGACCTAGATTCAAATATTATCATTGGACAACATCGTTATTAGGCCTCAT
CCTCTGTATAGCTTTGATGTTCGTATCCAGCTGGTACTATGCGTTGGTGG
CTATTGCTTTAGCTGGCGGCATTTACAAATACATCGAATATCGCGGTGCA
ACAAAGGAATGGGGGGACGCCACCAGAGGGTTTCAAATGTCGGCGGCAAA
GAAAGCAATTTTACAATTAGGTGATAAACCTGTCCATACGAAAAATTGGC
GCCCACAAATTCTTATATTGATCAATCTTGATGAAACTTGCACGAAAATG
TCCCATCCGAAACTCATAGATTTCTGCTCCCAATTGAAAGCCGGACGTGG
CTTAACCATAGTTGGAACTGTTATTGAAGGAGAATTGGGTGATAAAAAGA
GCGATTCTATTATTGCAAAAGAAACTTTAAATCGAGCTATTCGTGATAAA
CAGATTAAAGGATTAAGCGAAATCGTTGTCGCTTCAAATGTTATAGAAGC
TATTAAAATATTATGTCAAACCTCAGGTCTTGGTAATCTAAGACACAATT
GCGTTATGGTTTCATTCCCAGAAGAGTGGGCCAACGATAAGAGCCACATT
AAATGGAAACAGTTTATGGCAACCTTAAGAGTTTGTCAAGCCAGCGATTT
GGCAGTTCTTGTACCGAAGGGAATAGATCAATTTCCATATCGTAAATCTA
AAAAACAGTCAGCAAACGTTCCAAGTGACGGACCCGTAGAAATGACTGGT
ACTGTTGATATGTGGTGTATTGTACATGACGGAGGTCTTCTGTTATTACT
GGCACATTTATTGATAAGAGCGCGTGCTTGGGGAAAATGTGAATTGCGGG
TTTTTGTTGTTGCCAGTGAAAAAGACAACACAATCACCTTGAAAAAGGAA
GTCATCCGGTTTGTTTACGATCTGCGAATAAATGCTCAAGTTGAAGTAGT
TGAATTGGATCAAGCAGATATTTCTGCATACGCTTTCCAACGAACTATGG
ATCTAAATATGCGGAAAAAAATTCTTGAAGACATGAATCTTCGAACTGTC
GACACAAAGCGCGAATTTCAAGCGATTCTCGACAAACACCATGTAGCTGC
CGGAGAGCTCACGATGATGACGAATATTAATGACACTAGCCCCGATCTTC
CATTCTCACCGTTACCGACAATAAATGTTGACGTTCACGAAAATCAACCA
ACTGAGAAAACGGAAGAGACAGTATTAGATGAAGATAATCATCCAGATGA
AAACAAGGAATCTATGAATTCTCATATTTCAAAATTAAAATCGTCCAATA
CTGAACAAACACTGATTCTTACTCCAGATTCCACACCGCTCCAGAACCAG
AGGTCAGTGACTTTTGCCGCTCCGACTCTAGATCTGAATAATGCTCGTGG
CGATATGGCTCCTGAAAAAATCAATGAGTTCACATTTTCACCGGGTGCTA
TGCATGGATTAAAGTTCGATTTCAGCCCTTCTCTTGGCCATAAAAACAGA
CAAACAAAAACCGGCAGAAGGATGCATAGTGCGGTGCGATTAAACGAAAT
GATTCGGAGCCGATCAAGCAATGCCAGCCTAATACTTGTCAATCTCCCAG
GTCCATCCAAAAATGAATCTAGTGATTATAACTATATGCAATACGTTGAA
ACATTGACCGATGGTCTTGCTCGAGTACTTTTTGTCAGAGGAACTGGTAA
AGAGGTGATCACTGCATTTGCTGAATAGACCCAAGCTGTCAAATAGAGAC
GATGATAATTATTATATTATTGCATGCATAGTTGTGTTGATTCACCAAAG
ATGCGTATTATAACAAAATTTTATGAACGAA

protein sequence of SMED30003346-orf-1

>SMED30003346-orf-1 ID=SMED30003346-orf-1|Name=SMED30003346-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1175bp
MNEARFVVHRTSEVINSENDLSSVAAEKAAHYVVESKVENSENITKEATP
LTDEEIEMSDDEEPAGDSKFDLYEEEFHTRPPMANIFNKLAQYTGGIEPT
SGETKKLKEKSSNKLGTILGVYFPCLQNIFGVLFFLRLVWIIGVSGTALG
FVIVFVCCCSTMLTVLSMSAIATNGKVPAGGSYFMISRSLGPEFGGTVGI
LFYFGTTVAAAMYIIGAIEVMLKYIVPKASLFGPIELDENIMFNNTRVYG
VILLCIMMSCVFIGIKFVSKFSTISLACVLCSILCVYIGIFVANPSRSLR
VCMLGDRLLNRSVVLDKEANNTFRCTKDINGPLYKLYCRNGTKDLNDEAC
KYFKENNVTEFPAIPGFYRPLLKDGFFQNYYTTKGSLYGEPGKEPHLDKG
QSPSVSDIYISFTILIGIFFPSVTGIMAGSNRSGDLANPQFSIPLGSIAA
CATTSFVYLSAVIFFSATVEHTVLRDKFGGSVGEGLIVAKLAWPNEWVIL
IGSFLSTVGAGLQSLTGAPRLLQAIAQDNVIPFLEVFKVTTKRGEPLRAQ
ALTFFISFLGVLIASIDSIAPLITMFFLMCYGFVNLATALNGFLQEPSWR
PRFKYYHWTTSLLGLILCIALMFVSSWYYALVAIALAGGIYKYIEYRGAT
KEWGDATRGFQMSAAKKAILQLGDKPVHTKNWRPQILILINLDETCTKMS
HPKLIDFCSQLKAGRGLTIVGTVIEGELGDKKSDSIIAKETLNRAIRDKQ
IKGLSEIVVASNVIEAIKILCQTSGLGNLRHNCVMVSFPEEWANDKSHIK
WKQFMATLRVCQASDLAVLVPKGIDQFPYRKSKKQSANVPSDGPVEMTGT
VDMWCIVHDGGLLLLLAHLLIRARAWGKCELRVFVVASEKDNTITLKKEV
IRFVYDLRINAQVEVVELDQADISAYAFQRTMDLNMRKKILEDMNLRTVD
TKREFQAILDKHHVAAGELTMMTNINDTSPDLPFSPLPTINVDVHENQPT
EKTEETVLDEDNHPDENKESMNSHISKLKSSNTEQTLILTPDSTPLQNQR
SVTFAAPTLDLNNARGDMAPEKINEFTFSPGAMHGLKFDFSPSLGHKNRQ
TKTGRRMHSAVRLNEMIRSRSSNASLILVNLPGPSKNESSDYNYMQYVET
LTDGLARVLFVRGTGKEVITAFAE*

Aliases

The feature 'slc12a-4' has the following synonyms


Synonym
slc12a-4

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **molecular function**
Term	Definition
GO:0022857	transmembrane transporter activity
GO:0015377	cation:chloride symporter activity
GO:0015379	potassium:chloride symporter activity
GO:0005215	transporter activity

Vocabulary: **biological process**
Term	Definition
GO:0071805	potassium ion transmembrane transport
GO:0006811	ion transport
GO:0055085	transmembrane transport
GO:0006810	transport
GO:0071477	cellular hypotonic salinity response
GO:1902476	chloride transmembrane transport

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000017	photoreceptor neuron
PLANA:0000020	protonephridia
PLANA:0000047	collecting duct
PLANA:0000053	distal tubule
PLANA:0000099	neuron
PLANA:0000101	muscle cell
PLANA:0000418	head
PLANA:0003116	parenchymal cell

Vocabulary: **INTERPRO**
Term	Definition
IPR004842	SLC12A_fam
IPR030364	KCC3
IPR000076	KCL_cotranspt
IPR004841	AA-permease/SLC12A_dom
IPR018491	SLC12_C

Vocabulary: **cellular component**
Term	Definition
GO:0016021	integral component of membrane
GO:0016020	membrane
GO:0005887	integral component of plasma membrane

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000076	K/Cl co-transporter	PRINTS	PR01081	KCLTRNSPORT	coord: 294..311 score: 42.86 coord: 532..548 score: 47.06 coord: 558..566 score: 65.08 coord: 260..272 score: 53.85
IPR004841	Amino acid permease/ SLC12A domain	PFAM	PF00324	AA_permease	coord: 120..292 e-value: 2.8E-18 score: 65.8 coord: 410..687 e-value: 7.0E-45 score: 153.5
IPR018491	SLC12A transporter, C-terminal	PFAM	PF03522	SLC12	coord: 702..830 e-value: 9.8E-14 score: 51.2 coord: 1099..1171 e-value: 2.7E-14 score: 53.1 coord: 848..916 e-value: 1.4E-10 score: 40.8
None	No IPR available	GENE3D	G3DSA:1.20.1740.10		coord: 117..303 e-value: 3.2E-6 score: 28.1 coord: 391..663 e-value: 7.2E-22 score: 79.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 997..1026
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 997..1023
None	No IPR available	PANTHER	PTHR11827	SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS	coord: 1075..1172 coord: 19..981
None	No IPR available	TMHMM	TMhelix		coord: 572..594
None	No IPR available	TMHMM	TMhelix		coord: 547..566
None	No IPR available	TMHMM	TMhelix		coord: 271..293
None	No IPR available	TMHMM	TMhelix		coord: 138..157
None	No IPR available	TMHMM	TMhelix		coord: 445..467
None	No IPR available	TMHMM	TMhelix		coord: 196..218
None	No IPR available	TMHMM	TMhelix		coord: 164..186
None	No IPR available	TMHMM	TMhelix		coord: 115..134
None	No IPR available	TMHMM	TMhelix		coord: 615..637
None	No IPR available	TMHMM	TMhelix		coord: 408..430
None	No IPR available	TMHMM	TMhelix		coord: 248..265
IPR004842	SLC12A transporter family	TIGRFAM	TIGR00930	TIGR00930	coord: 62..1172 e-value: 6.2E-294 score: 975.7
IPR030364	K/Cl co-transporter 3	PANTHER	PTHR11827:SF66	SOLUTE CARRIER FAMILY 12 MEMBER 6	coord: 19..981
IPR030364	K/Cl co-transporter 3	PANTHER	PTHR11827:SF66	SOLUTE CARRIER FAMILY 12 MEMBER 6	coord: 1075..1172