APH domain-containing protein

Overview
NameAPH domain-containing protein
Smed IDSMED30003322
Length (bp)1323
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of APH domain-containing protein (SMED30003322) t-SNE clustered cells

Violin plots show distribution of expression levels for APH domain-containing protein (SMED30003322) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of APH domain-containing protein (SMED30003322) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for APH domain-containing protein (SMED30003322) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003322

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30003322SMESG000024756.1 SmedASXL_011451SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
parenchymal cellSMED30003322SMESG000024756.1 dd_Smed_v4_9386_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of APH domain-containing protein vs. Ensembl Human
Match: HYKK (hydroxylysine kinase [Source:HGNC Symbol;Acc:HGNC:34403])

HSP 1 Score: 74.3294 bits (181), Expect = 6.230e-14
Identity = 81/386 (20.98%), Postives = 156/386 (40.41%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFL---LSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI---------ADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLN-NHKIFSEQW 1300
            KP  S +Q    +   +   +   V+ L SYD + + V  + +   P +Y+ KI+N+  S++    +  + I+  L+  G     +  +K             N   L    + + +KS+L   L+Y+ G  I    +   L ++I +   +L   +    +   +   R                    N IWN+KN  L+   +        + I++  +  ++++++  + +      HGD ND NILI         A+    G +D+ D +    V++++I I  ++       ES     +G + L G+  E+   L  ++   L + V +R   SL    Y+ +       YL+ T KTGW+ L+ + +   K   E W
Sbjct:   14 KPTFSEEQASALVESVFGL-KVSKVRPLPSYDDQNFHVYVSKTKDGPTEYVLKISNTKASKNPDLIEVQNHIIMFLKAAGFPTASVCHTKGD-----------NTASLVSVDSGSEIKSYLVRLLTYLPGRPIAELPVSPQLLYEIGKLAAKLDKTLQRFHHPKLSSLHRE-------------------NFIWNLKNVPLLEKYLYALGQNRNREIVEHVIHLFKEEVMTKLSHFRECINHGDLNDHNILIESSKSASGNAEYQVSGILDFGDMSYGYYVFEVAITIMYMMI------ESKSPIQVGGHVLAGF--ESITPLTAVEKGALFLLVCSRFCQSLVMAAYSCQLYPENKDYLMVTAKTGWKHLQQMFDMGQKAVEEIW 360
[Close]
BLAST of APH domain-containing protein vs. Ensembl Human
Match: HYKK (hydroxylysine kinase [Source:HGNC Symbol;Acc:HGNC:34403])

HSP 1 Score: 74.3294 bits (181), Expect = 6.230e-14
Identity = 81/386 (20.98%), Postives = 156/386 (40.41%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFL---LSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI---------ADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLN-NHKIFSEQW 1300
            KP  S +Q    +   +   +   V+ L SYD + + V  + +   P +Y+ KI+N+  S++    +  + I+  L+  G     +  +K             N   L    + + +KS+L   L+Y+ G  I    +   L ++I +   +L   +    +   +   R                    N IWN+KN  L+   +        + I++  +  ++++++  + +      HGD ND NILI         A+    G +D+ D +    V++++I I  ++       ES     +G + L G+  E+   L  ++   L + V +R   SL    Y+ +       YL+ T KTGW+ L+ + +   K   E W
Sbjct:   14 KPTFSEEQASALVESVFGL-KVSKVRPLPSYDDQNFHVYVSKTKDGPTEYVLKISNTKASKNPDLIEVQNHIIMFLKAAGFPTASVCHTKGD-----------NTASLVSVDSGSEIKSYLVRLLTYLPGRPIAELPVSPQLLYEIGKLAAKLDKTLQRFHHPKLSSLHRE-------------------NFIWNLKNVPLLEKYLYALGQNRNREIVEHVIHLFKEEVMTKLSHFRECINHGDLNDHNILIESSKSASGNAEYQVSGILDFGDMSYGYYVFEVAITIMYMMI------ESKSPIQVGGHVLAGF--ESITPLTAVEKGALFLLVCSRFCQSLVMAAYSCQLYPENKDYLMVTAKTGWKHLQQMFDMGQKAVEEIW 360
[Close]
BLAST of APH domain-containing protein vs. Ensembl Fly
Match: CG31751 (gene:FBgn0086909 transcript:FBtr0081093)

HSP 1 Score: 86.6557 bits (213), Expect = 3.839e-18
Identity = 87/385 (22.60%), Postives = 170/385 (44.16%), Query Frame = 2 
Query:  152 GEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPL-------KYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI------ADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL 1267
            G+++    + +P V  + VE  L + +  + +  VKE+V+YD R + V    + + PL        Y+ KI NS +S+   F D  + +L +L  + +   + + +  GK    +  + +N       G +N ++  LL +I G++     + K L ++    L RL   + +  +  +                       S  ++W +++   +R  +   +   +++I    +  +E ++L  + ++    IHGD N+ NI++       +    G ID+ D + S  ++++ I +  ++    D        + G   L GY + N   +   ++ +L   V  R   SL  G YT+  L PTN YLL TQ+ GW+LL+ L
Sbjct:   53 GDLLKPGSDVRPKVEPEDVESLLRRLYGITIS-EVKEIVAYDDRNFFVKEDSNVKNPLIVTHCPHGYVLKILNSLDSKKEDFVDAQNQMLLYLGKHSVKCPRPVANATGKY---YSVERLN-------GNSNVVR--LLEFIPGEIFHQVPVTKHLLYRSGEYLARLDRALKNFTHQAYE----------------------SHKTLWMLQSVPELRQFLYVVKDQEQRLICDEVIDAFEAKVLSQLPSMEHQIIHGDFNEQNIVVEQVPNQTEYTIKGVIDFGDTSKSPLIFEIGIALTYMILQAND-------LANGGIFLSGYTSLNP--IENSELALLKYCVTARFVQSLVMGLYTHT-LHPTNEYLLVTQEKGWKLLQKL 392
[Close]
BLAST of APH domain-containing protein vs. Ensembl Fly
Match: CG31751 (gene:FBgn0086909 transcript:FBtr0081092)

HSP 1 Score: 86.6557 bits (213), Expect = 3.839e-18
Identity = 87/385 (22.60%), Postives = 170/385 (44.16%), Query Frame = 2 
Query:  152 GEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPL-------KYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI------ADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL 1267
            G+++    + +P V  + VE  L + +  + +  VKE+V+YD R + V    + + PL        Y+ KI NS +S+   F D  + +L +L  + +   + + +  GK    +  + +N       G +N ++  LL +I G++     + K L ++    L RL   + +  +  +                       S  ++W +++   +R  +   +   +++I    +  +E ++L  + ++    IHGD N+ NI++       +    G ID+ D + S  ++++ I +  ++    D        + G   L GY + N   +   ++ +L   V  R   SL  G YT+  L PTN YLL TQ+ GW+LL+ L
Sbjct:   53 GDLLKPGSDVRPKVEPEDVESLLRRLYGITIS-EVKEIVAYDDRNFFVKEDSNVKNPLIVTHCPHGYVLKILNSLDSKKEDFVDAQNQMLLYLGKHSVKCPRPVANATGKY---YSVERLN-------GNSNVVR--LLEFIPGEIFHQVPVTKHLLYRSGEYLARLDRALKNFTHQAYE----------------------SHKTLWMLQSVPELRQFLYVVKDQEQRLICDEVIDAFEAKVLSQLPSMEHQIIHGDFNEQNIVVEQVPNQTEYTIKGVIDFGDTSKSPLIFEIGIALTYMILQAND-------LANGGIFLSGYTSLNP--IENSELALLKYCVTARFVQSLVMGLYTHT-LHPTNEYLLVTQEKGWKLLQKL 392
[Close]
BLAST of APH domain-containing protein vs. Ensembl Fly
Match: CG31751 (gene:FBgn0086909 transcript:FBtr0344023)

HSP 1 Score: 86.6557 bits (213), Expect = 3.839e-18
Identity = 87/385 (22.60%), Postives = 170/385 (44.16%), Query Frame = 2 
Query:  152 GEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPL-------KYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI------ADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL 1267
            G+++    + +P V  + VE  L + +  + +  VKE+V+YD R + V    + + PL        Y+ KI NS +S+   F D  + +L +L  + +   + + +  GK    +  + +N       G +N ++  LL +I G++     + K L ++    L RL   + +  +  +                       S  ++W +++   +R  +   +   +++I    +  +E ++L  + ++    IHGD N+ NI++       +    G ID+ D + S  ++++ I +  ++    D        + G   L GY + N   +   ++ +L   V  R   SL  G YT+  L PTN YLL TQ+ GW+LL+ L
Sbjct:   53 GDLLKPGSDVRPKVEPEDVESLLRRLYGITIS-EVKEIVAYDDRNFFVKEDSNVKNPLIVTHCPHGYVLKILNSLDSKKEDFVDAQNQMLLYLGKHSVKCPRPVANATGKY---YSVERLN-------GNSNVVR--LLEFIPGEIFHQVPVTKHLLYRSGEYLARLDRALKNFTHQAYE----------------------SHKTLWMLQSVPELRQFLYVVKDQEQRLICDEVIDAFEAKVLSQLPSMEHQIIHGDFNEQNIVVEQVPNQTEYTIKGVIDFGDTSKSPLIFEIGIALTYMILQAND-------LANGGIFLSGYTSLNP--IENSELALLKYCVTARFVQSLVMGLYTHT-LHPTNEYLLVTQEKGWKLLQKL 392
[Close]
BLAST of APH domain-containing protein vs. Ensembl Fly
Match: CG31751 (gene:FBgn0086909 transcript:FBtr0445413)

HSP 1 Score: 86.6557 bits (213), Expect = 3.839e-18
Identity = 87/385 (22.60%), Postives = 170/385 (44.16%), Query Frame = 2 
Query:  152 GEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPL-------KYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI------ADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL 1267
            G+++    + +P V  + VE  L + +  + +  VKE+V+YD R + V    + + PL        Y+ KI NS +S+   F D  + +L +L  + +   + + +  GK    +  + +N       G +N ++  LL +I G++     + K L ++    L RL   + +  +  +                       S  ++W +++   +R  +   +   +++I    +  +E ++L  + ++    IHGD N+ NI++       +    G ID+ D + S  ++++ I +  ++    D        + G   L GY + N   +   ++ +L   V  R   SL  G YT+  L PTN YLL TQ+ GW+LL+ L
Sbjct:   53 GDLLKPGSDVRPKVEPEDVESLLRRLYGITIS-EVKEIVAYDDRNFFVKEDSNVKNPLIVTHCPHGYVLKILNSLDSKKEDFVDAQNQMLLYLGKHSVKCPRPVANATGKY---YSVERLN-------GNSNVVR--LLEFIPGEIFHQVPVTKHLLYRSGEYLARLDRALKNFTHQAYE----------------------SHKTLWMLQSVPELRQFLYVVKDQEQRLICDEVIDAFEAKVLSQLPSMEHQIIHGDFNEQNIVVEQVPNQTEYTIKGVIDFGDTSKSPLIFEIGIALTYMILQAND-------LANGGIFLSGYTSLNP--IENSELALLKYCVTARFVQSLVMGLYTHT-LHPTNEYLLVTQEKGWKLLQKL 392
[Close]
BLAST of APH domain-containing protein vs. Ensembl Zebrafish
Match: hykk.2 (hydroxylysine kinase, tandem duplicate 2 [Source:NCBI gene;Acc:559560])

HSP 1 Score: 64.3142 bits (155), Expect = 6.913e-11
Identity = 81/368 (22.01%), Postives = 154/368 (41.85%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQG-TTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI-ADGLR----VGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL 1267
            KP +S  QV   + + +  + ++ V+ L SYD + + V  ++ G    +++ K+ NS +S ++   +  +  +  L   G+ AQ  + +  G +++  EE         D G  T      LL+Y+ G  I   T    + + + +    L   +  + + + ++  R               E F    IW + +  L+   +   +    Q I+K  +  Y+ Q++P +        HGD ND N+++  DG       G +D+ D +    +++   L  +++ + ++SP  LD   +G   + G+  E+   LNE +   L   V  R   SL    YT         YL+ T + G R L  L
Sbjct:    7 KPNLSHSQVTHVVKRLYGLTASV-VRPLPSYDDQNFYVAPSEGG----EFVLKVMNSADSENVAVIELQTQSMNFLHQRGLPAQTALPTLTG-QLMSLEE--------FDCGFGTQTYLIRLLTYLPGTTIAKITCSPQILYDVGKMAATLDTVLLQMEHPNTSVLQR---------------ERF----IWKLTSIPLLNQYVHVMDGDPVQKIVKGVVEKYQVQVMPKLPLFRECINHGDFNDHNLIVKPDGPSKYKISGILDFADMSCGYFIFE---LAITIMYMMIESPTPLD---VGGPVVAGW--ESVFPLNEAERDSLYWLVMCRFCQSLVLARYTVIQQPENEEYLMITSRKGLRHLSRL 333
[Close]
BLAST of APH domain-containing protein vs. Ensembl Zebrafish
Match: hykk.2 (hydroxylysine kinase, tandem duplicate 2 [Source:NCBI gene;Acc:559560])

HSP 1 Score: 64.3142 bits (155), Expect = 6.913e-11
Identity = 81/368 (22.01%), Postives = 154/368 (41.85%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQG-TTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI-ADGLR----VGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL 1267
            KP +S  QV   + + +  + ++ V+ L SYD + + V  ++ G    +++ K+ NS +S ++   +  +  +  L   G+ AQ  + +  G +++  EE         D G  T      LL+Y+ G  I   T    + + + +    L   +  + + + ++  R               E F    IW + +  L+   +   +    Q I+K  +  Y+ Q++P +        HGD ND N+++  DG       G +D+ D +    +++   L  +++ + ++SP  LD   +G   + G+  E+   LNE +   L   V  R   SL    YT         YL+ T + G R L  L
Sbjct:    7 KPNLSHSQVTHVVKRLYGLTASV-VRPLPSYDDQNFYVAPSEGG----EFVLKVMNSADSENVAVIELQTQSMNFLHQRGLPAQTALPTLTG-QLMSLEE--------FDCGFGTQTYLIRLLTYLPGTTIAKITCSPQILYDVGKMAATLDTVLLQMEHPNTSVLQR---------------ERF----IWKLTSIPLLNQYVHVMDGDPVQKIVKGVVEKYQVQVMPKLPLFRECINHGDFNDHNLIVKPDGPSKYKISGILDFADMSCGYFIFE---LAITIMYMMIESPTPLD---VGGPVVAGW--ESVFPLNEAERDSLYWLVMCRFCQSLVLARYTVIQQPENEEYLMITSRKGLRHLSRL 333
[Close]
BLAST of APH domain-containing protein vs. Ensembl Zebrafish
Match: hykk.1 (hydroxylysine kinase, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-131120-24])

HSP 1 Score: 61.2326 bits (147), Expect = 6.866e-10
Identity = 75/343 (21.87%), Postives = 143/343 (41.69%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQG-TTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI-ADGLR----VGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYT 1192
            KP +S  Q    L + +  + ++ ++ L SY  + + V S++ G    +Y+ K  NS +S+D+      +  +  L   G+ AQ  + +  G +++  EE         D G  T      LL+Y+ G  I   +I+  + + I +    L   +  + + + ++  R                    N IW++ +  L+   I   +    Q ++K  +  Y+ Q+ P +    +   HGD ND N+L+  DG       G +D+ D +    +++   L  ++  +  +SP  LD   +G   + G+  E+   LNE +   L   +  R   SL    YT
Sbjct:    7 KPNLSHSQATDILKRLYGLTTSV-IRPLPSYIDQNFYVASSEGG----EYVLKAMNSADSQDIAVIKVQTYSMAFLNQRGLPAQIALPTLTG-QMMSLEE--------IDCGFGTQTYLVRLLTYMPGTTICKISINTQILYDIGKMAATLDTVLLQMEHPNISVLQRD-------------------NFIWSLTSIPLLYQYIHVMDGDPVQKVVKAVIEQYQVQVRPKLPLFRKCINHGDLNDRNLLVKPDGPSKYAISGILDFADMSCGYFIFE---LAITIRYMMTESPNPLD---VGGPVVAGW--ESVFPLNEAERDSLYWLILCRFCQSLVIARYT 308
[Close]
BLAST of APH domain-containing protein vs. Ensembl Zebrafish
Match: hykk.1 (hydroxylysine kinase, tandem duplicate 1 [Source:ZFIN;Acc:ZDB-GENE-131120-24])

HSP 1 Score: 61.2326 bits (147), Expect = 6.898e-10
Identity = 75/343 (21.87%), Postives = 143/343 (41.69%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQG-TTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI-ADGLR----VGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYT 1192
            KP +S  Q    L + +  + ++ ++ L SY  + + V S++ G    +Y+ K  NS +S+D+      +  +  L   G+ AQ  + +  G +++  EE         D G  T      LL+Y+ G  I   +I+  + + I +    L   +  + + + ++  R                    N IW++ +  L+   I   +    Q ++K  +  Y+ Q+ P +    +   HGD ND N+L+  DG       G +D+ D +    +++   L  ++  +  +SP  LD   +G   + G+  E+   LNE +   L   +  R   SL    YT
Sbjct:   12 KPNLSHSQATDILKRLYGLTTSV-IRPLPSYIDQNFYVASSEGG----EYVLKAMNSADSQDIAVIKVQTYSMAFLNQRGLPAQIALPTLTG-QMMSLEE--------IDCGFGTQTYLVRLLTYMPGTTICKISINTQILYDIGKMAATLDTVLLQMEHPNISVLQRD-------------------NFIWSLTSIPLLYQYIHVMDGDPVQKVVKAVIEQYQVQVRPKLPLFRKCINHGDLNDRNLLVKPDGPSKYAISGILDFADMSCGYFIFE---LAITIRYMMTESPNPLD---VGGPVVAGW--ESVFPLNEAERDSLYWLILCRFCQSLVIARYT 313
[Close]
BLAST of APH domain-containing protein vs. Ensembl Xenopus
Match: hykk (hydroxylysine kinase [Source:NCBI gene;Acc:549168])

HSP 1 Score: 86.2705 bits (212), Expect = 7.783e-18
Identity = 92/381 (24.15%), Postives = 161/381 (42.26%), Query Frame = 2 
Query:  176 NEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRT-YVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSF-LLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNIL---------IADGLRV-GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLNNHK 1282
            N KPA+  +Q  + +   +    + N+K L SYD +  Y+ +         +Y+ KI NS +SR     +  + ++  L NNG+ AQK + + NG+ +           +  D G+T   ++  LL+Y+ G  +       ++ + + +    +             +   +F    + C   G         IWN+ N  L++  +   +    Q I++  ++ YE  +LPN+    +   HGD ND NIL         I +  +V G +D+ D +    +++L+I I  ++       ES D    G Y L G+  E+   L   +   L   V  R + SL    Y+         YL+ T KTGW+ L+ L N  K
Sbjct:   21 NNKPALREEQAVQLIESLYGLKVS-NIKALPSYDDQNFYIKSCCKDPSGCCEYVMKITNSEDSRYGELLEAQTCVMVFLCNNGVPAQKPVCTINGQNL---------SLETIDYGSTIEEQAVRLLTYLPGTPLAKIVATPEMLFDVGKMAANIDK-----------MLAENFQHPNKTCFERGQF-------IWNLSNTPLLQKYVHAVKEEELQNIIEGVITQYETFVLPNLSCFRKCINHGDLNDHNILVEKNSSPGSIQEQYKVSGILDFSDMSYGYYIFELAITIMYMMI------ESNDPLHAGGYVLAGF--ESVIPLTAEEKDALFFLVNCRFSQSLVMARYSVLLCPENEEYLMITAKTGWKHLKTLHNMGK 365
[Close]
BLAST of APH domain-containing protein vs. Ensembl Mouse
Match: Hykk (hydroxylysine kinase 1 [Source:MGI Symbol;Acc:MGI:2443139])

HSP 1 Score: 78.5666 bits (192), Expect = 1.831e-15
Identity = 81/393 (20.61%), Postives = 165/393 (41.98%), Query Frame = 2 
Query:  170 KLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDS---RTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFL---LSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI-----ADGLRV----GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLN-NHKIFSEQW 1300
            + + KP  +  Q    +   + F  +  ++ L SY+    R ++    ++   P++Y+ KI+N+  S+     +  + ++  L+  G     + ++K             N   L    + + +KS+L   L+Y+ G  I    I     ++I R   +L   ++   +   ++  R                    N IWN+KN  L+   +        + I++  +  ++++++  + +      HGD ND NIL+     A G  V    G +D+ D +    V++++I+I  ++       ES +   +G + L G+  E+   L  ++ + L + V +R + SL    Y+ +       YL+ T KTGW+ L+ L +   K   E W
Sbjct:   10 QASAKPVFTEAQASALVESVFGFKVS-KIQPLPSYEDQNFRVHIARGKETTDDPVEYVLKISNTESSQTPELIEMQNHVIMFLRAAGFPTASVCRTKGD-----------NTISLISIDSGSGVKSYLVRMLTYLPGRPIAEVAISHQQLYEIGRLAAQLDKALEEFHHPKLSLFHRE-------------------NFIWNLKNVPLLEKYMGALSQSRNREIVEQVIRMFKEEVMTKLSHFRECINHGDLNDHNILVDLSKSASGEGVHQVSGILDFGDMSYGYYVFEVAIVIMYMMI------ESTNPIQVGGHILAGF--ESVIPLTAVERQALFLLVCSRFSQSLVMAAYSCQLYPENKEYLMITAKTGWKHLQQLFDMGQKAVEEIW 363
[Close]
BLAST of APH domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6PB06|HYKK_XENLA (Hydroxylysine kinase OS=Xenopus laevis OX=8355 GN=hykk PE=2 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 3.103e-19
Identity = 94/376 (25.00%), Postives = 167/376 (44.41%), Query Frame = 2 
Query:  176 NEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRT-YVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSF-LLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNIL---------IADGLRV-GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL 1267
            N KPA+S  Q  + +   +   + +N+K L SYD +  Y+ + ++      +Y+ KI NS +SR     +  + ++  L +NG+ AQK + +KNG+ +           +  D G+T   ++  LL+Y+ G  +       ++ + I +    +   +D        +   +F    + C   G         IWN+ N SL+R      +    Q I++  ++ YE  +LPN+    +   HGD ND NIL         I +  +V G +D+ D +    +++L+I I  ++       ES D    G Y L G+  ++   L + +   L   V  R + SL    Y+ +       YL+ T KTGW+ L+ L
Sbjct:   12 NNKPALSEDQAVQLIESLYGV-KVLNIKPLPSYDDQNFYIKSCSEDPNGCCEYVMKITNSEDSRYGELLEAQTSVMVFLCSNGVPAQKPVFTKNGQSL---------SLETIDYGSTIQKQAVRLLTYLPGTPLARVVATPEILFDIGK----MAANIDK-------MLAENFLHPNKTCFERGQF-------IWNLSNTSLLRKYAHAVKETELQKIIEDVITQYETFVLPNLNCFRKCINHGDLNDHNILVEKTSSPGSIQEQYKVSGILDFSDMSFGYYIFELAITIMYMMI------ESNDPLHAGGYVLAGF--QSVIPLTDEEKDALFFLVNCRFSQSLVMARYSVQLCPENEEYLMITAKTGWKHLQTL 351
[Close]
BLAST of APH domain-containing protein vs. UniProt/SwissProt
Match: sp|Q5U5V2|HYKK_MOUSE (Hydroxylysine kinase OS=Mus musculus OX=10090 GN=Hykk PE=1 SV=2)

HSP 1 Score: 78.5666 bits (192), Expect = 1.281e-14
Identity = 81/393 (20.61%), Postives = 165/393 (41.98%), Query Frame = 2 
Query:  170 KLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDS---RTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFL---LSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI-----ADGLRV----GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLN-NHKIFSEQW 1300
            + + KP  +  Q    +   + F  +  ++ L SY+    R ++    ++   P++Y+ KI+N+  S+     +  + ++  L+  G     + ++K             N   L    + + +KS+L   L+Y+ G  I    I     ++I R   +L   ++   +   ++  R                    N IWN+KN  L+   +        + I++  +  ++++++  + +      HGD ND NIL+     A G  V    G +D+ D +    V++++I+I  ++       ES +   +G + L G+  E+   L  ++ + L + V +R + SL    Y+ +       YL+ T KTGW+ L+ L +   K   E W
Sbjct:   10 QASAKPVFTEAQASALVESVFGFKVS-KIQPLPSYEDQNFRVHIARGKETTDDPVEYVLKISNTESSQTPELIEMQNHVIMFLRAAGFPTASVCRTKGD-----------NTISLISIDSGSGVKSYLVRMLTYLPGRPIAEVAISHQQLYEIGRLAAQLDKALEEFHHPKLSLFHRE-------------------NFIWNLKNVPLLEKYMGALSQSRNREIVEQVIRMFKEEVMTKLSHFRECINHGDLNDHNILVDLSKSASGEGVHQVSGILDFGDMSYGYYVFEVAIVIMYMMI------ESTNPIQVGGHILAGF--ESVIPLTAVERQALFLLVCSRFSQSLVMAAYSCQLYPENKEYLMITAKTGWKHLQQLFDMGQKAVEEIW 363
[Close]
BLAST of APH domain-containing protein vs. UniProt/SwissProt
Match: sp|A2RU49|HYKK_HUMAN (Hydroxylysine kinase OS=Homo sapiens OX=9606 GN=HYKK PE=1 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 2.992e-13
Identity = 81/386 (20.98%), Postives = 156/386 (40.41%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFL---LSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI---------ADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLN-NHKIFSEQW 1300
            KP  S +Q    +   +   +   V+ L SYD + + V  + +   P +Y+ KI+N+  S++    +  + I+  L+  G     +  +K             N   L    + + +KS+L   L+Y+ G  I    +   L ++I +   +L   +    +   +   R                    N IWN+KN  L+   +        + I++  +  ++++++  + +      HGD ND NILI         A+    G +D+ D +    V++++I I  ++       ES     +G + L G+  E+   L  ++   L + V +R   SL    Y+ +       YL+ T KTGW+ L+ + +   K   E W
Sbjct:   14 KPTFSEEQASALVESVFGL-KVSKVRPLPSYDDQNFHVYVSKTKDGPTEYVLKISNTKASKNPDLIEVQNHIIMFLKAAGFPTASVCHTKGD-----------NTASLVSVDSGSEIKSYLVRLLTYLPGRPIAELPVSPQLLYEIGKLAAKLDKTLQRFHHPKLSSLHRE-------------------NFIWNLKNVPLLEKYLYALGQNRNREIVEHVIHLFKEEVMTKLSHFRECINHGDLNDHNILIESSKSASGNAEYQVSGILDFGDMSYGYYVFEVAITIMYMMI------ESKSPIQVGGHVLAGF--ESITPLTAVEKGALFLLVCSRFCQSLVMAAYSCQLYPENKDYLMVTAKTGWKHLQQMFDMGQKAVEEIW 360
[Close]
BLAST of APH domain-containing protein vs. UniProt/SwissProt
Match: sp|A5PJU6|HYKK_BOVIN (Hydroxylysine kinase OS=Bos taurus OX=9913 GN=HYKK PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 5.818e-10
Identity = 82/392 (20.92%), Postives = 156/392 (39.80%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVV------TSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFL---LSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIADGLRV---------GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLN-NHKIFSEQW 1300
            KP+ S  Q    +   +   +   ++ L SYD + + V       +TD    P + + KI+N+  S+     +  + I+  L+  G     + ++K             N   L    + + +KS+L   L+Y+ G  I    I   L ++I R   +L   ++   +   +   R                    N IWN+K+  L+   +        + I++  +  ++D+++ ++ +      HGD ND NILI               G +D+ D +    V++++I I  ++       ES     +G + L G+  E+   L  ++   L + V +R   SL    Y+ +       YL+ T KTGW+ L+ + +   K   E W
Sbjct:   14 KPSFSEVQASALVESVFGL-KVSKIQPLPSYDDQNFHVCIARTKVTTDG---PNECVLKISNTESSKTPDLIEVQTHIIMFLRAAGFPTASVCRTKGD-----------NVSSLVSVDSGSEVKSYLVRLLTYLPGRPIAEIPIGPQLLYEIGRLAAKLDKTLEKFHHPKLSSLHRE-------------------NFIWNLKSVPLLEKYLYALGQNRNREIVEQVIQLFKDEVMTSLSHFRECINHGDLNDHNILIVSSESAFGDAVYQVSGILDFDDMSYGYYVFEVAITIMYMMI------ESKTPIQVGGHVLAGF--ESVVPLTPVERGALFLLVCSRFCQSLVLAAYSCQLYPENEEYLMITAKTGWKHLQQMFDMGRKAVEEIW 363
[Close]
BLAST of APH domain-containing protein vs. UniProt/SwissProt
Match: sp|A8WFT6|HYKK_DANRE (Hydroxylysine kinase OS=Danio rerio OX=7955 GN=hykk PE=2 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 9.634e-10
Identity = 82/368 (22.28%), Postives = 153/368 (41.58%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQG-TTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI-ADGLR----VGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL 1267
            KP +S  QV   + + +  + ++ V+ L SYD + + V  ++ G    +++ K+ NS +S ++   +  +  +  L   G+ AQ  + +  G +++  EE         D G  T      LL+Y+ G  I   T    + + + +    L   +  + + +  +  R               E F    IW + +  L+   +   +    Q I+K  +  Y+ Q++P +        HGD ND N+L+  DG       G +D+ D +    +++   L  +++ + ++SP  LD   +G   + G+  E+   LNE +   L   V  R   SL    YT         YL+ T + G R L  L
Sbjct:    7 KPNLSHSQVTDVVKRLYGLTASV-VRPLPSYDDQNFYVAPSEGG----EFVLKVMNSADSENVAVIELQTQSMNFLHQRGLPAQTALPTLTG-QLMSLEE--------FDCGFGTQIYLVRLLTYLPGTTIAKITCSPQILYDVGKMAATLDTVLLQMEHPNTRVLQR---------------ERF----IWKLTSIPLLNQYVHVMDGDPVQKIVKGVIEKYQVQVMPKLPLFRECINHGDFNDHNLLVKPDGPSKYKISGILDFADMSCGYFIFE---LAITIMYMMIESPTPLD---VGGPVVAGW--ESVFPLNEAERDSLYWLVMCRFCQSLVLARYTVIQQPENEEYLMITSRKGLRHLSRL 333
[Close]
BLAST of APH domain-containing protein vs. TrEMBL
Match: A0A1B6CDI4 (APH domain-containing protein (Fragment) OS=Clastoptera arizonana OX=38151 GN=g.41283 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 2.963e-25
Identity = 112/400 (28.00%), Postives = 181/400 (45.25%), Query Frame = 2 
Query:  152 GEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTY-----VVTSTDSGR--QPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSF---LLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIA-----DGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLNNHKIFSEQWKL 1306
            GE+I      KP +SV Q      + + F    ++ EL  YD + Y      V S    R  Q   Y+FK+ NS +S+ L + D  + +L HL+ NG  A ++ K+  G E    E        +T +   N +KS    LL+YIEG+L+ + +I   LC+ + R                   ++ +   K +   +  Y    S +S+W +KN   I + I   +   K  ++K  +  +E  +L  + +LT G IHGD N+ NIL       +      +D+ D  +S  V++L+I +  ++    +          G++ L GY       L + +I +LPI V  RI  SL  G Y+  + DP N Y+L+TQK+GW +L  L  N     + W L
Sbjct:   10 GEVI------KPELSVDQAIELSQKLYGFEIT-SIMELNGYDDKNYHLKIAAVKSNSHIREVQHSGYVFKVMNSLDSKKLDYVDGQNKLLLHLEKNGFSAPRIQKNIKG-EYYSVEN-------ITSKKDKNDIKSHVVRLLTYIEGELLRDVSITDLLCFDVGR-------------------SVATLDQKLETFKHKAY---DSYDSVWMLKNVPQILNFIYVIKDQRKVALVKKVVREFEKNVLTKLNDLTSGMIHGDANEQNILCVKQPDDEWCVNAILDYGDSHLSAYVFNLAITMTYIILQCKNLQH-------GRFVLSGYTTIKP--LPKKEIDLLPICVSARICQSLVLGAYS-YFQDPGNEYVLSTQKSGWEMLFKLSENFDSLPDLWGL 362
[Close]
BLAST of APH domain-containing protein vs. TrEMBL
Match: A0A443SK05 (Hydroxylysine kinase-like isoform X2 OS=Leptotrombidium deliense OX=299467 GN=B4U80_03839 PE=4 SV=1)

HSP 1 Score: 113.235 bits (282), Expect = 4.298e-24
Identity = 100/379 (26.39%), Postives = 179/379 (47.23%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIADGLR----VGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL-LNNHKIFSEQWK 1303
            KP ++ ++  + L Q + + R +++KEL SYD R Y+V S  S    L++  KI NS ES      +  + I QH+   G+     I +K GK +   +    +D       T N ++  LL +I G ++      + L  +  + L RLTN +    Y + A+  RS                    SIWN+KNA  +   +       +  +++  L  +E+++L  + +L +  IHGD N+ NIL+            ID+ DC  + +V+DLS+    ++        ++D      + ++GY       + + ++ ++P+ ++ R++ SL  G Y+  +LDP N Y L++ KTGW LLE +  +N     ++WK
Sbjct:   15 KPEITKEKAAQLLFQLYGY-RAVDIKELNSYDDRNYLV-SVLSNENVLQFTLKITNSLESNIPGLIEAFNEIGQHISEKGLTVPVPILNKEGKYLCKIKLSENDD-------TINSVR--LLKFIPGQMLKEVAYSERLLIETGQLLARLTNALQD--YRNEALQKRS--------------------SIWNLKNAVKVEEFLFAISDECRIQMIRNVLKEFENKVLCKIDSLNQSIIHGDFNEQNILVRKVNEHYEPYAVIDFGDCHFAPQVFDLSMACAYVMLDCT----TMDPLIAPAFVIQGY--TQIRQITDEELALIPVCIKARLSQSLVMGAYS-YFLDPNNEYTLSSSKTGWILLEKMTASNDSQLLQKWK 353
[Close]
BLAST of APH domain-containing protein vs. TrEMBL
Match: A0A1I8GHX0 (APH domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030284g2 PE=4 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 8.580e-22
Identity = 108/407 (26.54%), Postives = 186/407 (45.70%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVT---------STDSGRQPLKYIFKIANSTESR-DLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSF-----------LLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANS----IWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQIL-------PNMKNLTRGFIHGDPNDMNILIADGLRV--GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYS-IGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLNNHKIFSEQ 1297
            +P V+ +Q  +   + + F+  +  KELVSYD R ++VT         S ++ ++P  ++ KIANS ES+ D+    C   ++Q L   G+  Q ++K+ +G          I D KL     T P  S            +L+Y+ G  +    +  D+   +++E          +G +D+ +T R F            L +  AN+    +W++ +A  +   + +     +Q +++  ++ +   ++             T+ FIHGDPN+ NIL+     V  G +DWQD   +  V+DL++LI     +   +  +LD +  +    ++GY +E    L E +++ LP+ V TR A SL  G Y+    DP N YLL T    W  L+ L N HK   EQ
Sbjct:   12 RPLVTREQAVKLCQELYNFN-AVTCKELVSYDDRNFLVTGNFIGDTSDSVETAQEPHSFVLKIANSLESQGDILEQQCR--LMQSLSEAGLKVQSVMKTVSGA---------IVDKKLL----TTPADSSGRMVDRRHAVRMLTYLPGVPLGELPLTADMLESVAKE----------IGRVDWHLT-RHF------------LTDPGANAAELNLWSLLSAPKLVDFVQYVHGEARQRMVREIVAQFNSVVVGAAGGGSEATAQFTKAFIHGDPNESNILVDGNPPVFSGLLDWQDSHRAYAVFDLALLIMYTSFVQAKTDPTLDPFRLVVPPLIRGYTSEFK--LAECELRHLPLLVATRFAQSLTLGAYSYAR-DPGNDYLLTT-ACSWSTLDRLWNGHKNRLEQ 375
[Close]
BLAST of APH domain-containing protein vs. TrEMBL
Match: A0A1I8GG10 (APH domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig024598g1 PE=4 SV=1)

HSP 1 Score: 106.301 bits (264), Expect = 2.166e-21
Identity = 107/407 (26.29%), Postives = 186/407 (45.70%), Query Frame = 2 
Query:  182 KPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVT---------STDSGRQPLKYIFKIANSTESR-DLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSF-----------LLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANS----IWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQIL-------PNMKNLTRGFIHGDPNDMNILIADGLRV--GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYS-IGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLNNHKIFSEQ 1297
            +P V+ +Q  +   + + F+  +  KELVSYD R ++VT         S ++ ++P  ++ KIANS ES+ D+    C   ++Q L   G+  Q ++K+ +G          I D KL     T P  S            +L+Y+ G  +    +  D+   +++E          +G +D+ +T R F            L +  AN+    +W++ +A  +   + +     +Q +++  ++ +   ++             T+ FIHGDPN+ NIL+     V  G +DWQD   +  V+DL++LI     +   +  ++D +  +    ++GY +E    L E +++ LP+ V TR A SL  G Y+    DP N YLL T    W  L+ L N HK   EQ
Sbjct:   12 RPLVTREQAVKLCQELYNFN-AVTCKELVSYDDRNFLVTGNFIGNTSDSVETAQEPHSFVLKIANSLESQGDILEQQCR--LMQSLSEAGLKVQSVMKTVSGT---------IVDKKLL----TTPADSSGRMVDRRHAVRMLTYLPGVPLGELPLTADMLESVAKE----------IGRVDWHLT-RHF------------LTDPGANAAELNLWSLLSAPKLVDFVQYVHGEARQRMVREIVAQFNSVVVGAAGAKSEATAQFTKAFIHGDPNESNILVDGNPPVFSGLLDWQDSHRAYAVFDLALLIMYTSFVQAKTDPTVDPFRLVVPPLIRGYTSEFK--LAESELRHLPLLVATRFAQSLTLGAYSYAR-DPGNDYLLTT-ACSWSTLDRLWNGHKNRLEQ 375
[Close]
BLAST of APH domain-containing protein vs. TrEMBL
Match: A0A210R6W0 (Hydroxylysine kinase OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT16961 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 5.312e-21
Identity = 111/425 (26.12%), Postives = 187/425 (44.00%), Query Frame = 2 
Query:  101 NNNDKYEIKVQILSI*MGEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPL-------KYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSF---LLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIAD---------GLRV----GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLNNHK-IFSEQWK 1303
            NNN+  + KV +     GE+I      KP +    VE  + + +     ++ KEL SYD + Y VT TD    P         YI KI NS +S+     +  + +L HL+   +   + + + +G E++  E+  +   K      T P  ++   LL +I GD++ N+     L + + + + +  N              + F   F    +L          +W++   S ++  +   +  + + ++   +S +E  +LP    L +G IHGD N+ NIL+ +           RV    G +D+ D T S  V+D++I I  +      S E LDA   G + L G +       N++++  L I V  R+  SL  G Y N Y DP+N Y+L T   GW LL  L N  K +  ++W+
Sbjct:    3 NNNEATKPKVVLQE--KGEVI------KPMIPEGVVEELVRKIYGLDV-VSYKELNSYDDKNYHVTVTDRSSNPHIPEVWTHGYILKILNSMDSKRPVIVEAQNSLLSHLRERNMSVPETVLTISG-ELMSLEK--VFAEKDPKDPDTAPYGTYIVRLLKFIPGDILFNKPCTPGLLYNVGKFMGKFLNAT------------KGFHHSFYDTHSL----------LWSLLEFSKLKEFVFVVKDENDKRVVDEIISAFESDVLPRCDQLQKGVIHGDLNEQNILVVEVPGQDDVTPESRVYDVSGLLDFMDVTDSYCVFDVAINIAYMSFTCEPS-EQLDA---GGHILAGMVPVFP--FNDVELDALQISVCARLCQSLVLGAYAN-YQDPSNSYVLTTAAKGWPLLHKLWNTPKQVLKDRWQ 386
[Close]
BLAST of APH domain-containing protein vs. Ensembl Cavefish
Match: hykk.1 (hydroxylysine kinase [Source:NCBI gene;Acc:103024473])

HSP 1 Score: 77.411 bits (189), Expect = 3.595e-15
Identity = 85/377 (22.55%), Postives = 160/377 (42.44%), Query Frame = 2 
Query:  176 NEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSF---LLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI----ADGLRV-GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLNNHK 1282
            + KP +S+ Q      + +  +   +++ L SYD + + V S +SG    +Y+ K+ NS  S++L   +  + I+  L   G+ AQ  + +  G +++  EE         D G    L+ +   LL+++ G  +   T      +++ +    +   +  + +   +   R                    N IWN+ +  L+   +P F+    Q ++K     Y   I P +    +   HGD ND N+L+    + G R+ G +D+ D +    V++L+I I  L+       ES     +G   + G+  E+   LN  + + + + V +R   SL  G +  +       YL+ T + G RLL+ L N  K
Sbjct:    5 DSKPYLSLSQATELTGRLFGLTVT-SIRPLPSYDDQNFHVESAESG----QYVLKVMNSAASQNLHLLEVQTHIMNFLWEKGLPAQTAMPTVTG-QLMSLEE--------IDCGFG--LQKYMVRLLTFLPGTPMAKVTYTSQFLFEVGKMAATVDKLLLQMEHPHLSALQRE-------------------NFIWNLASVPLLEQFLPLFDGEPIQEVVKRVFEQYRINIAPKIHCFRKCINHGDFNDHNVLVESDASSGYRISGILDFGDMSNGYFVFELAITIMYLMI------ESSSPLEVGGPVIAGF--ESVIPLNSEEREAVYLLVLSRFCQSLALGRHAVQQQPENEEYLMITARPGLRLLQLLWNTGK 338
[Close]
BLAST of APH domain-containing protein vs. Ensembl Cavefish
Match: hykk.1 (hydroxylysine kinase [Source:NCBI gene;Acc:103024473])

HSP 1 Score: 77.0258 bits (188), Expect = 4.600e-15
Identity = 85/377 (22.55%), Postives = 160/377 (42.44%), Query Frame = 2 
Query:  176 NEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSF---LLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI----ADGLRV-GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLNNHK 1282
            + KP +S+ Q      + +  +   +++ L SYD + + V S +SG    +Y+ K+ NS  S++L   +  + I+  L   G+ AQ  + +  G +++  EE         D G    L+ +   LL+++ G  +   T      +++ +    +   +  + +   +   R                    N IWN+ +  L+   +P F+    Q ++K     Y   I P +    +   HGD ND N+L+    + G R+ G +D+ D +    V++L+I I  L+       ES     +G   + G+  E+   LN  + + + + V +R   SL  G +  +       YL+ T + G RLL+ L N  K
Sbjct:   18 DSKPYLSLSQATELTGRLFGLTVT-SIRPLPSYDDQNFHVESAESG----QYVLKVMNSAASQNLHLLEVQTHIMNFLWEKGLPAQTAMPTVTG-QLMSLEE--------IDCGFG--LQKYMVRLLTFLPGTPMAKVTYTSQFLFEVGKMAATVDKLLLQMEHPHLSALQRE-------------------NFIWNLASVPLLEQFLPLFDGEPIQEVVKRVFEQYRINIAPKIHCFRKCINHGDFNDHNVLVESDASSGYRISGILDFGDMSNGYFVFELAITIMYLMI------ESSSPLEVGGPVIAGF--ESVIPLNSEEREAVYLLVLSRFCQSLALGRHAVQQQPENEEYLMITARPGLRLLQLLWNTGK 351
[Close]
BLAST of APH domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001601.1 (pep scaffold:Pmarinus_7.0:GL489249:35:5705:-1 gene:ENSPMAG00000001444.1 transcript:ENSPMAT00000001601.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 59.3066 bits (142), Expect = 3.807e-10
Identity = 49/193 (25.39%), Postives = 81/193 (41.97%), Query Frame = 2 
Query:  749 IWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIADGLRV--------------------GFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL 1267
            IWN+ N  L+   +   +    Q +++  ++ + ++I PN+   TR  +HGD ND NIL+ +   +                    G +D+ D      VYD+++ I  ++       ES     +G + + GY  E+   L E +   +   V +R   SL    YT         YLL T +TGW  L  L
Sbjct:   70 IWNLSNTHLLESYLFALDGDPNQGLVREVIAKFREEIQPNLHKFTRCIVHGDLNDHNILVEETGPIPAVQSSETGEPAAGSHYKLSGVLDFNDMMQGYCVYDIAMAIMYMMI------ESSRPMDVGGHIMAGY--ESVRPLTEDERGAVYWLVASRFCQSLVLARYTILQHPENADYLLTTARTGWERLRRL 254
[Close]
BLAST of APH domain-containing protein vs. Ensembl Nematostella
Match: EDO36201 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJD3])

HSP 1 Score: 87.0409 bits (214), Expect = 7.550e-19
Identity = 90/351 (25.64%), Postives = 154/351 (43.87%), Query Frame = 2 
Query:  254 VKELVSYDSRTYVVTS--TDSGRQPLK---YIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNP---LKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSA-NSIWNIKNASLIRHLIPH-FECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIADGL---RVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDL 1267
            VKEL+SYD R + +     +  ++P     ++ K++N   S+        S +L +L    I       S+NG            DYK+  +   N        L SY+ G L+    + +D+ + +   +  +     H    DF+ T  S             + E S  N IWNI+NA  + + + H F+C  K  ++   +  +++ IL  +  + +  IHGD N+ NIL+ + L     GFID+ D   S +V DL I +  ++ +   +       ++G +   GY +E    L + +++ML      R + SL  G Y + Y+   N YLL +QK  W+ L  L
Sbjct:   37 VKELISYDDRNFYLQGFIQNEEQEPANLRGFLLKVSNPAFSKSQSILKGNSDLLLYLSKRDITCPVPYSSRNG------------DYKVLSKDEDNADGACAVRLFSYVSGSLLEKVALTEDVLYDLGASVASM-----HKAMKDFSNTYPS-------------IHELSRDNFIWNIRNAPRVVNKLSHVFDCGVKLDLINTAMKRFQN-ILSKLDTIEKQIIHGDMNEKNILVQEKLDKNTFGFIDFNDFYFSNRVVDLGIALAYIMMLPQVNSHLTRPQAVG-HMFAGYQSEWP--LPKEEVEMLGAIAAARCSQSLVVGAYQHAYVHLDNEYLLVSQKGSWKALNSL 353
[Close]
BLAST of APH domain-containing protein vs. Ensembl Medaka
Match: hykk.1 (hydroxylysine kinase [Source:NCBI gene;Acc:101157673])

HSP 1 Score: 73.559 bits (179), Expect = 5.647e-14
Identity = 86/375 (22.93%), Postives = 163/375 (43.47%), Query Frame = 2 
Query:  158 IIPIKL---NEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSF---LLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI-----ADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNL-YLLNTQKTG 1246
            + P K+   + KP  S  QV   L + ++ + +  ++ L SYD + + V + + G    +Y+FKI NS +S++    +  +  +  L  NG+ AQ ++ + +G++I            L +      L+ +   LL+Y+ G  I    +  +L ++  +    +   +  + + + +I  R               E F    IW++ N  L+   I   +    Q ++K  + +Y+  + P   +      HGD ND+N+L+      D    G +D+ D      +++L+I I  +++      E  D   +G   L G+  E+   LNEM+   L + V +R   SL    Y+   L P N  YL+ T + G
Sbjct:    4 LFPAKMPPKHAKPEFSESQVSEILQRLYKLTSS-EIRSLPSYDDQNFYVAAVEGG----EYVFKIMNSEDSKNPAITEVQTYAMSFLCQNGLPAQTVLPTVSGQQI-----------SLEEMDCGFGLQKYLVRLLTYLPGVTISKVPLIPNLLYEAGKMAATMDTVLQKMEHPNISILQR---------------EGF----IWSLSNVPLLEAYIGPLDGDPLQELVKSIIHHYKTSVEPKRCHFRTCINHGDFNDLNVLVQADENGDYKISGILDFGDMNYGYYIHELAITIMYMMT------EHPDPVQVGGPILAGW--ESVIPLNEMEKDCLYLLVMSRFCQSLVLARYSVT-LHPENAEYLMITSRKG 334
[Close]
BLAST of APH domain-containing protein vs. Ensembl Medaka
Match: hykk.1 (hydroxylysine kinase [Source:NCBI gene;Acc:101157673])

HSP 1 Score: 73.1738 bits (178), Expect = 7.232e-14
Identity = 84/366 (22.95%), Postives = 159/366 (43.44%), Query Frame = 2 
Query:  176 NEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSF---LLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI-----ADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNL-YLLNTQKTG 1246
            + KP  S  QV   L + ++ + +  ++ L SYD + + V + + G    +Y+FKI NS +S++    +  +  +  L  NG+ AQ ++ + +G++I            L +      L+ +   LL+Y+ G  I    +  +L ++  +    +   +  + + + +I  R               E F    IW++ N  L+   I   +    Q ++K  + +Y+  + P   +      HGD ND+N+L+      D    G +D+ D      +++L+I I  +++      E  D   +G   L G+  E+   LNEM+   L + V +R   SL    Y+   L P N  YL+ T + G
Sbjct:    5 HAKPEFSESQVSEILQRLYKLTSS-EIRSLPSYDDQNFYVAAVEGG----EYVFKIMNSEDSKNPAITEVQTYAMSFLCQNGLPAQTVLPTVSGQQI-----------SLEEMDCGFGLQKYLVRLLTYLPGVTISKVPLIPNLLYEAGKMAATMDTVLQKMEHPNISILQR---------------EGF----IWSLSNVPLLEAYIGPLDGDPLQELVKSIIHHYKTSVEPKRCHFRTCINHGDFNDLNVLVQADENGDYKISGILDFGDMNYGYYIHELAITIMYMMT------EHPDPVQVGGPILAGW--ESVIPLNEMEKDCLYLLVMSRFCQSLVLARYSVT-LHPENAEYLMITSRKG 326
[Close]
BLAST of APH domain-containing protein vs. Ensembl Medaka
Match: hykk.1 (hydroxylysine kinase [Source:NCBI gene;Acc:101157673])

HSP 1 Score: 64.3142 bits (155), Expect = 6.040e-11
Identity = 78/339 (23.01%), Postives = 145/339 (42.77%), Query Frame = 2 
Query:  257 KELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFL---LSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILI-----ADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNL-YLLNTQKTG 1246
            K  V YD + + V + + G    +Y+FKI NS +S++    +  +  +  L  NG+ AQ ++ + +G++I            L +      L+ +L   L+Y+ G  I    +  +L ++  +    +   +  + + + +I  R               E F    IW++ N  L+   I   +    Q ++K  + +Y+  + P   +      HGD ND+N+L+      D    G +D+ D      +++L+I I  +++      E  D   +G   L G+  E+   LNEM+   L + V +R   SL    Y+   L P N  YL+ T + G
Sbjct:   25 KARVHYDDQNFYVAAVEGG----EYVFKIMNSEDSKNPAITEVQTYAMSFLCQNGLPAQTVLPTVSGQQI-----------SLEEMDCGFGLQKYLVRLLTYLPGVTISKVPLIPNLLYEAGKMAATMDTVLQKMEHPNISILQR---------------EGF----IWSLSNVPLLEAYIGPLDGDPLQELVKSIIHHYKTSVEPKRCHFRTCINHGDFNDLNVLVQADENGDYKISGILDFGDMNYGYYIHELAITIMYMMT------EHPDPVQVGGPILAGW--ESVIPLNEMEKDCLYLLVMSRFCQSLVLARYSVT-LHPENAEYLMITSRKG 320
[Close]
BLAST of APH domain-containing protein vs. Planmine SMEST
Match: SMESG000024756.1 (SMESG000024756.1)

HSP 1 Score: 706.442 bits (1822), Expect = 0.000e+0
Identity = 362/386 (93.78%), Postives = 365/386 (94.56%), Query Frame = 2 
Query:  149 MGEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLNNHKIFSEQWKL 1306
            MGEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDS RQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPIND KLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCW+ISRELYRLTNCVDH                  ICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIADGLRVGFIDWQDCTISLKVYDLSILIFSL+SIG+DSPESLD YSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLNNHKIFSEQWKL
Sbjct:    1 MGEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSERQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDDKLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCWRISRELYRLTNCVDH------------------ICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIADGLRVGFIDWQDCTISLKVYDLSILIFSLMSIGIDSPESLDVYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTGWRLLEDLLNNHKIFSEQWKL 368
[Close]
BLAST of APH domain-containing protein vs. Planmine SMEST
Match: SMESG000024756.1 (SMESG000024756.1)

HSP 1 Score: 664.07 bits (1712), Expect = 0.000e+0
Identity = 343/370 (92.70%), Postives = 347/370 (93.78%), Query Frame = 2 
Query:  149 MGEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDYKLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIADGLRVGFIDWQDCTISLKVYDXXXXXXXXXXXXXDSPESLDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKT-GWRL 1255
            MGEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDS RQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPIND KLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCW+ISRELYRLTNCVDH                  ICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIADGLRVGFIDWQDCTISLKVYDLSILIFSL+SIG+DSPESLD YSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKT  W+L
Sbjct:    1 MGEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVTSTDSERQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKSKNGKEILDFEEKPINDDKLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDLCWRISRELYRLTNCVDH------------------ICSNLGYLEEFSANSIWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIHGDPNDMNILIADGLRVGFIDWQDCTISLKVYDLSILIFSLMSIGIDSPESLDVYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNKYLDPTNLYLLNTQKTEQWKL 352
The following BLAST results are available for this feature:
BLAST of APH domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
HYKK6.230e-1420.98hydroxylysine kinase [Source:HGNC Symbol;Acc:HGNC:... [more]
HYKK6.230e-1420.98hydroxylysine kinase [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of APH domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of APH domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 4
Match NameE-valueIdentityDescription
CG317513.839e-1822.60gene:FBgn0086909 transcript:FBtr0081093[more]
CG317513.839e-1822.60gene:FBgn0086909 transcript:FBtr0081092[more]
CG317513.839e-1822.60gene:FBgn0086909 transcript:FBtr0344023[more]
CG317513.839e-1822.60gene:FBgn0086909 transcript:FBtr0445413[more]
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BLAST of APH domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
hykk.26.913e-1122.01hydroxylysine kinase, tandem duplicate 2 [Source:N... [more]
hykk.26.913e-1122.01hydroxylysine kinase, tandem duplicate 2 [Source:N... [more]
hykk.16.866e-1021.87hydroxylysine kinase, tandem duplicate 1 [Source:Z... [more]
hykk.16.898e-1021.87hydroxylysine kinase, tandem duplicate 1 [Source:Z... [more]
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BLAST of APH domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hykk7.783e-1824.15hydroxylysine kinase [Source:NCBI gene;Acc:549168][more]
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BLAST of APH domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Hykk1.831e-1520.61hydroxylysine kinase 1 [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of APH domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6PB06|HYKK_XENLA3.103e-1925.00Hydroxylysine kinase OS=Xenopus laevis OX=8355 GN=... [more]
sp|Q5U5V2|HYKK_MOUSE1.281e-1420.61Hydroxylysine kinase OS=Mus musculus OX=10090 GN=H... [more]
sp|A2RU49|HYKK_HUMAN2.992e-1320.98Hydroxylysine kinase OS=Homo sapiens OX=9606 GN=HY... [more]
sp|A5PJU6|HYKK_BOVIN5.818e-1020.92Hydroxylysine kinase OS=Bos taurus OX=9913 GN=HYKK... [more]
sp|A8WFT6|HYKK_DANRE9.634e-1022.28Hydroxylysine kinase OS=Danio rerio OX=7955 GN=hyk... [more]
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BLAST of APH domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1B6CDI42.963e-2528.00APH domain-containing protein (Fragment) OS=Clasto... [more]
A0A443SK054.298e-2426.39Hydroxylysine kinase-like isoform X2 OS=Leptotromb... [more]
A0A1I8GHX08.580e-2226.54APH domain-containing protein OS=Macrostomum ligna... [more]
A0A1I8GG102.166e-2126.29APH domain-containing protein OS=Macrostomum ligna... [more]
A0A210R6W05.312e-2126.12Hydroxylysine kinase OS=Mizuhopecten yessoensis OX... [more]
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BLAST of APH domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
hykk.13.595e-1522.55hydroxylysine kinase [Source:NCBI gene;Acc:1030244... [more]
hykk.14.600e-1522.55hydroxylysine kinase [Source:NCBI gene;Acc:1030244... [more]
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BLAST of APH domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000001601.13.807e-1025.39pep scaffold:Pmarinus_7.0:GL489249:35:5705:-1 gene... [more]
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BLAST of APH domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of APH domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO362017.550e-1925.64Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of APH domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
hykk.15.647e-1422.93hydroxylysine kinase [Source:NCBI gene;Acc:1011576... [more]
hykk.17.232e-1422.95hydroxylysine kinase [Source:NCBI gene;Acc:1011576... [more]
hykk.16.040e-1123.01hydroxylysine kinase [Source:NCBI gene;Acc:1011576... [more]
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BLAST of APH domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000024756.10.000e+093.78SMESG000024756.1[more]
SMESG000024756.10.000e+092.70SMESG000024756.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003322 ID=SMED30003322|Name=APH domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1323bp
ATTCAGACATCCATTCAAGTACAATGAAATTTGAACTCTAGTAAATACGT
ACCAACTAAGGCATGCTTACCCGTGTTTGTGTGCGTCCGTAAAGAAAATT
AATAATAACGACAAATATGAAATCAAAGTTCAAATCTTATCAATCTGAAT
GGGTGAGATAATTCCAATCAAGCTCAACGAGAAGCCTGCCGTTTCGGTTC
AGCAAGTGGAACGATTTCTCAATCAAAATTGGGAGTTCTCGAGAAATATG
AATGTTAAAGAGCTCGTTTCATACGATTCCAGGACATATGTTGTGACCTC
GACTGATTCTGGGCGCCAGCCGTTGAAATACATTTTCAAAATTGCAAACT
CCACCGAATCTCGAGATTTGCGCTTTTTCGATTGTCTATCAATTATTCTT
CAACATTTACAAAATAACGGCATTCTAGCTCAGAAGCTGATCAAAAGCAA
GAATGGCAAGGAGATTCTTGATTTCGAAGAGAAACCCATAAACGACTATA
AACTGACAGACCAAGGGACAACGAACCCTCTGAAATCATTTCTATTGTCT
TACATTGAAGGGGACTTGATTCTTAACAGAACTATTGATAAAGATTTATG
TTGGAAAATTTCGAGAGAGTTATATCGACTAACCAACTGCGTGGATCACG
TAGGATATTTAGATTTTGCAATCACAATTCGTAGTTTTGGATCTAAATTT
CAGATATGTTCTAATTTGGGATATCTTGAAGAGTTCTCTGCTAACAGCAT
TTGGAATATAAAGAACGCATCACTGATTCGACACCTTATTCCTCATTTTG
AATGTCCTCACAAACAGATGATTCTAAAGCTGACATTGTCAAATTATGAG
GACCAGATTCTACCTAATATGAAAAATTTAACAAGAGGCTTCATTCACGG
CGATCCAAACGATATGAATATTTTAATAGCTGATGGACTTAGAGTTGGCT
TCATTGACTGGCAAGATTGTACAATATCTCTCAAGGTCTACGATCTCAGT
ATCCTTATATTTTCATTATTGAGTATCGGCCTTGACAGTCCGGAATCATT
AGACGCGTACTCGATTGGCAAGTACTGCTTAAAAGGTTATTTAGCTGAGA
ATACTGATTTCCTCAATGAAATGGATATCAAGATGTTGCCAATATTCGTA
CAAACTAGAATAGCCTTATCCCTCTGCACAGGATACTACACAAATAAATA
TCTAGATCCGACCAATTTATATTTATTGAATACTCAGAAAACTGGTTGGA
GATTATTAGAGGACCTGTTAAATAATCATAAGATCTTTTCAGAACAATGG
AAACTTTGAACAATTTATTGTCA
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protein sequence of SMED30003322-orf-1

>SMED30003322-orf-1 ID=SMED30003322-orf-1|Name=SMED30003322-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=387bp
MGEIIPIKLNEKPAVSVQQVERFLNQNWEFSRNMNVKELVSYDSRTYVVT
STDSGRQPLKYIFKIANSTESRDLRFFDCLSIILQHLQNNGILAQKLIKS
KNGKEILDFEEKPINDYKLTDQGTTNPLKSFLLSYIEGDLILNRTIDKDL
CWKISRELYRLTNCVDHVGYLDFAITIRSFGSKFQICSNLGYLEEFSANS
IWNIKNASLIRHLIPHFECPHKQMILKLTLSNYEDQILPNMKNLTRGFIH
GDPNDMNILIADGLRVGFIDWQDCTISLKVYDLSILIFSLLSIGLDSPES
LDAYSIGKYCLKGYLAENTDFLNEMDIKMLPIFVQTRIALSLCTGYYTNK
YLDPTNLYLLNTQKTGWRLLEDLLNNHKIFSEQWKL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0016310phosphorylation
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR002575Aminoglycoside_PTrfase
IPR011009Kinase-like_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0016740transferase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002575Aminoglycoside phosphotransferasePFAMPF01636APHcoord: 37..291
e-value: 7.0E-13
score: 49.1
NoneNo IPR availableGENE3DG3DSA:3.90.1200.10coord: 193..365
e-value: 1.3E-8
score: 36.3
NoneNo IPR availablePANTHERPTHR21064:SF1HYDROXYLYSINE KINASEcoord: 10..378
NoneNo IPR availablePANTHERPTHR21064UNCHARACTERIZEDcoord: 10..378
IPR011009Protein kinase-like domain superfamilySUPERFAMILYSSF56112Protein kinase-like (PK-like)coord: 13..381