G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30003314
Length (bp)1086
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30003314) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30003314) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30003314) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30003314) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003314

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
headSMED30003314SMESG000022454.1 SmedASXL_008644SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
headSMED30003314SMESG000022454.1 dd_Smed_v6_23883_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: G4VR07 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma mansoni OX=6183 GN=Smp_204230 PE=4 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 5.139e-28
Identity = 129/364 (35.44%), Postives = 192/364 (52.75%), Query Frame = 2
Query:  104 LFTYYNYXXXXXXXXXXXXNTLAIFIFNHRLWRKSLTSIILKYLSVFE-----SLF-NATVLIKNSYDNYAIQSKSPN----FSSYESLL-----IDETFRLFIRL----FGIVFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIEXXXXXXXXXXXXPRLFEKYVVACAEPLWKIEGKVA----FLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKF---------RN------------------------------RNSNNKV-----RPQPLGDKXXXXXXXXXXXXESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDXXXXXXXXXXXXPTFK 994
            LF++Y    ++L  ++ +LN L + IFNH  W+KS+ S ILK LS+ E     SLF +A +  +NS     +   + N    + + +  +     I   F  FI      F +VF I RN +V +LAA+RY+ +C P+G+        +  I ++IF LA +I +PR+FE  +V C      I G V+     L +   Y  L+ G+V+FI QSGGPVI V +LS +++++I K +KF         RN                              RNSN        RP P GDK++F +C+ FFILE+P+FFSKIL  YL  ++  ++D TL V+AN+ IY+DS +N FIY+ SNP F+
Sbjct:   39 LFSFYRAIGLLLGPLNTILNGLCVHIFNHSSWKKSIMSRILKGLSIIELGMGLSLFIHALIFSQNSTTTSHLHQNTTNSIYPYHNEQDTIQTNAYIKTIFSFFIMTISSRFLVVFQISRNWSVVLLAAYRYDQICRPIGSPSAFPRERIRYILIIIFFLACLIVIPRIFESGIVVCY-----ISGNVSKDYPLLINYKLYQILYLGLVMFIVQSGGPVICVCVLSAFVIRIIAKRRKFHREKEQRSARNLLRRQTMEIENNQSNLKNNIINNYNNNPLKRNSNGLTKELTERPAPSGDKLMFAVCITFFILETPAFFSKILNPYLE-QDYPLIDLTLSVIANLLIYLDSTLNAFIYMASNPLFR 396          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3Q0KV19 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 5.334e-28
Identity = 129/364 (35.44%), Postives = 192/364 (52.75%), Query Frame = 2
Query:  104 LFTYYNYXXXXXXXXXXXXNTLAIFIFNHRLWRKSLTSIILKYLSVFE-----SLF-NATVLIKNSYDNYAIQSKSPN----FSSYESLL-----IDETFRLFIRL----FGIVFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIEXXXXXXXXXXXXPRLFEKYVVACAEPLWKIEGKVA----FLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKF---------RN------------------------------RNSNNKV-----RPQPLGDKXXXXXXXXXXXXESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDXXXXXXXXXXXXPTFK 994
            LF++Y    ++L  ++ +LN L + IFNH  W+KS+ S ILK LS+ E     SLF +A +  +NS     +   + N    + + +  +     I   F  FI      F +VF I RN +V +LAA+RY+ +C P+G+        +  I ++IF LA +I +PR+FE  +V C      I G V+     L +   Y  L+ G+V+FI QSGGPVI V +LS +++++I K +KF         RN                              RNSN        RP P GDK++F +C+ FFILE+P+FFSKIL  YL  ++  ++D TL V+AN+ IY+DS +N FIY+ SNP F+
Sbjct:   38 LFSFYRAIGLLLGPLNTILNGLCVHIFNHSSWKKSIMSRILKGLSIIELGMGLSLFIHALIFSQNSTTTSHLHQNTTNSIYPYHNEQDTIQTNAYIKTIFSFFIMTISSRFLVVFQISRNWSVVLLAAYRYDQICRPIGSPSAFPRERIRYILIIIFFLACLIVIPRIFESGIVVCY-----ISGNVSKDYPLLINYKLYQILYLGLVMFIVQSGGPVICVCVLSAFVIRIIAKRRKFHREKEQRSARNLLRRQTMEIENNQSNLKNNIINNYNNNPLKRNSNGLTKELTERPAPSGDKLMFAVCITFFILETPAFFSKILNPYLE-QDYPLIDLTLSVIANLLIYLDSTLNAFIYMASNPLFR 395          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183P9C9 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS10965 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 7.755e-28
Identity = 133/377 (35.28%), Postives = 194/377 (51.46%), Query Frame = 2
Query:  104 LFTYYNYXXXXXXXXXXXXNTLAIFIFNHRLWRKSLTSIILKYLSVFE-----SLF-NATVLIKNSYDNYAIQ----------SKSPNF-------------SSYESLLIDETFRLFIRL----FGIVFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIEXXXXXXXXXXXXPRLFEKYVVACAEPLWKIEGKVA----FLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKF---------RN-----------------------------RNSNNKV-----RPQPLGDKXXXXXXXXXXXXESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDXXXXXXXXXXXXPTFK 994
            LF++Y    ++L  ++ +LN L + IFNH LW+KS+ S ILK LS+ E     SLF +A +  +NS  +Y+ Q          S  PN+             S + +  I      FI      F + F I RN +V +LAA+RY+ +C P+G         +  I ++IF LA +I +PR+FE  +V C      I G V+     L +   Y  L+ G+V+FI QSGGPVI V +LS +++++I K +KF         RN                             RNSN        RP P GDK++F +C+ FFILE+P+FFSKIL  YL  ++  ++D TL V+AN+ IY+DS +N FIY+ SNP F+
Sbjct:   38 LFSFYRAIGLLLGPLNTILNGLCVHIFNHHLWKKSIMSRILKGLSIIELGMGLSLFIHALIFSQNSTSSYSNQNLFNINNTTNSIYPNYDIHINHLNQFQHDSIHTNAYIKTICSFFITTISSRFLVAFQISRNWSVVLLAAYRYDQICRPIGTSSAFPRERIRYILIIIFFLACLIVIPRIFESGIVVCY-----ISGNVSKDYPLLINYKLYQILYLGLVMFIVQSGGPVICVCVLSAFVIRIIAKRRKFHREKEQRSARNLLRRQTMEIENNQSNLKNNTNNNNTNFLQRNSNGLTKELTERPAPSGDKLMFAVCITFFILETPAFFSKILNPYLE-QDYPLIDLTLSVIANLLIYLDSTLNAFIYMASNPLFR 408          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A193KUR7 (GCR101 OS=Schmidtea mediterranea OX=79327 GN=gcr101 PE=2 SV=1)

HSP 1 Score: 121.709 bits (304), Expect = 8.601e-28
Identity = 118/339 (34.81%), Postives = 186/339 (54.87%), Query Frame = 2
Query:   38 IKVINCSKII--TNASGHVS--YSNLLFTYYNYXXXXXXXXXXXXNTLAIFIFNHRLWRKSLTSIILKYLSVFESLFNATVLIKNSYDNYAIQSKSPNFSSYESL-LIDETFRLFIRLFGI----VFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIEXXXXXXXXXXXXPRLFEKYVVACAEPLWKIEGKVAFLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFRNRNSN--NKVRPQPLGDKXXXXXXXXXXXXESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDXXXXXXXXXXXXPTFKLAFVQILKP 1021
            +++ NCS I+  +N + +VS   S LL+ ++N++ ++L  +S +LN   +FIF  ++WRKS  SI+LKYLS  E  FN +  I               F  Y S+    + F +F+   G       LICRN T+  LAA R+E +C P+ +++   ++   ++  + ++LA + A PR FE     C   L    G + +    +WY Y ++G ++FI Q+  PV    +LSF +++ I KMKKFR   S   +K      GD ++  +C  FFILE PSFFSK+L++YL        D  L  + N+ +Y+DS+IN F+YIISNPTF+     + +P
Sbjct:    1 MEIENCSDILETSNNAANVSASLSLLLYRFHNHALLILPILSMVLNGFCLFIFFDKIWRKSTLSILLKYLSATEIAFNFSGFI----------CTITKFIDYPSIGYFSKLFWVFVLQVGTFSVSTALICRNWTILHLAAFRFESICRPLSSRKLYTNKYSPRVLWLTYVLAGIFAFPRTFEYEPSFCKGELSLNPGLIKY----HWYEYGYQGSLIFIIQTAAPVAITSVLSFGVMKAIFKMKKFRQSCSKKFHKKHNAVSGDFLMIFICFAFFILEIPSFFSKVLKSYLMKHQKFTEDILLATMGNLLVYLDSVINIFVYIISNPTFRERSKNLFQP 325          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3SGW5 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS4169 PE=4 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 9.924e-27
Identity = 110/312 (35.26%), Postives = 159/312 (50.96%), Query Frame = 2
Query:  161 NTLAIFIFNHRLWRKSLTSIILKYLSVFESLFNATVLIKN----------SYDNYAIQSKSPNFSSYESLLID--ETFRLFIRLFGIVFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIEXXXXXXXXXXXXPRLFEKYVVACAEPLWKIEGKVAFLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFR-----------NRNSNNKVRPQ-----------PLGDKXXXXXXXXXXXXESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDXXXXXXXXXXXXPTFK 994
            N +++ IF HRLW+ S TS ILK LSVFE  F +++ +              DNY   + +       + L+      +  +  F   F I RN +V +LAA+RY+ +C  +G         +  I     +L+ + ALPRLFE     C  P   I  +   L  +  Y  L+ G+  FI QSGGPV+ V ILSF++++ I   +KFR           N+N +N    Q           P GDK++  LC +FFILE+P+FFSKIL +Y  S N   +D  + V+AN  IY+DS  N F+Y+ SNPTF+
Sbjct:   51 NGISLKIFGHRLWKNSSTSAILKSLSVFEICFGSSLFLHTINSKVLETYTRLDNYTTANLTEKERMVNAKLVAGMTVSQQILSGFLFAFQISRNWSVVLLAAYRYDAICRKLGTVSSFPRERIPWIMTTTMVLSGLFALPRLFEIKSFICI-PTVMIYQEEPLLAGNLMYQLLYLGIGTFIVQSGGPVLCVCILSFFVIRKIALRRKFRKQNQRTVFIAANKNEDNSGSAQQTATKVVERKLPSGDKLMLALCFMFFILETPAFFSKILSSY-TSTNYPTLDVYISVIANFLIYLDSTANVFVYMGSNPTFR 360          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000022454.1 (SMESG000022454.1)

HSP 1 Score: 587.415 bits (1513), Expect = 0.000e+0
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 2
Query:   32 MYIKVINCSKIITNASGHVSYSNLLFTYYNYXXXXXXXXXXXXNTLAIFIFNHRLWRKSLTSIILKYLSVFESLFNATVLIKNSYDNYAIQSKSPNFSSYESLLIDETFRLFIRLFGIVFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIEXXXXXXXXXXXXPRLFEKYVVACAEPLWKIEGKVAFLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFRNRNSNNKVRPQPLGDKXXXXXXXXXXXXESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDXXXXXXXXXXXXPTFKLAFVQILKPCFLAKSENVHQTTVIL 1069
            MYIKVINCSKIITNASGHVSYSNLLFTYYNYSSIVLSSISFLLNTLAIFIFNHRLWRKSLTSIILKYLSVFESLFNATVLIKNSYDNYAIQSKSPNFSSYESLLIDETFRLFIRLFGIVFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIELVIFLLALVIALPRLFEKYVVACAEPLWKIEGKVAFLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFRNRNSNNKVRPQPLGDKIIFPLCLIFFILESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDSIINFFIYIISNPTFKLAFVQILKPCFLAKSENVHQTTVIL
Sbjct:    1 MYIKVINCSKIITNASGHVSYSNLLFTYYNYSSIVLSSISFLLNTLAIFIFNHRLWRKSLTSIILKYLSVFESLFNATVLIKNSYDNYAIQSKSPNFSSYESLLIDETFRLFIRLFGIVFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIELVIFLLALVIALPRLFEKYVVACAEPLWKIEGKVAFLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFRNRNSNNKVRPQPLGDKIIFPLCLIFFILESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDSIINFFIYIISNPTFKLAFVQILKPCFLAKSENVHQTTVIL 346          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000063294.1 (SMESG000063294.1)

HSP 1 Score: 171.014 bits (432), Expect = 1.054e-49
Identity = 130/300 (43.33%), Postives = 187/300 (62.33%), Query Frame = 2
Query:  110 TYYNYXXXXXXXXXXXXNTLAIFIFNHRLWRKSLTSIILKYLSVFESLFNATVLIKNSYDNYAIQSKSPNFSSYESLLIDETFRLFIRLFGIVFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIEXXXXXXXXXXXXPRLFEKYVVACAEPLWKIEGKVAFLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFRNRN---SNNKVRPQPLGDKXXXXXXXXXXXXESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDXXXXXXXXXXXXPTFKLA 1000
            T Y Y +++L+ +S L NT A  +F   +W+KS+ S IL+ LS+FE LFN  VL++ S   + I+ + P   S   L+I        ++  IVFLI RN TV +LA +RYE VC PVG+ + I   N+      IF L+++IALPR+ E  +  C +P   +   V FL S  WY+YLH G++LF+ Q+GGPVI V  LS  +L+VI+KMKK R  N   ++ K  PQP GD++I  L   FF+LE+P+FFSKIL  Y   +    +D  + ++AN  IY+DSIIN  +Y+ISNPTF+++
Sbjct:   32 TIYKYITLILTPLSILSNTFAFVVFKESIWKKSVMSKILRMLSLFEILFNVNVLLEASI-RHKIEEQHP---SMVELVIFIYVITAAKVLMIVFLIARNWTVVILAGYRYEAVCRPVGSCKYIRYNNINLAFCFIFALSILIALPRMMETKIEIC-QPTLSVNRFVPFLISYKWYLYLHTGILLFLIQNGGPVILVCFLSSCVLKVIRKMKKIRKSNFNLADKKGSPQPSGDRLIVILSAAFFVLETPAFFSKILHHYFEYQGYYNLDIHIGLLANTLIYLDSIINVAVYVISNPTFRIS 326          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000023730.1 (SMESG000023730.1)

HSP 1 Score: 125.176 bits (313), Expect = 2.318e-31
Identity = 116/342 (33.92%), Postives = 189/342 (55.26%), Query Frame = 2
Query:   47 INCS---KIITNASGH-----VSYSNLLFTYYNYXXXXXXXXXXXXNTLAIFIFNHRLWRKSLTSIILKYLSVFESLFNATVLIKNSYDNYAIQS--KSPNFSSYESLLIDETFRLFIRLFGIV-FLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIEXXXXXXXXXXXXPRLFEKYVVACAEPLWKIEGKVAF---LDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFRN------RNSNNKVRPQPLGDKXXXXXXXXXXXXESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDXXXXXXXXXXXXPTFKLAFVQI 1012
            INCS   + + N S        +++  ++ ++NY+ +V+  IS + N   +FIF  ++W+KS+ SI+LKYLS  E +FN +  +      Y++      P+ + +  L     F L I  F +   LICRN T+  LA +R++ +C P+  K+     + +++  + ++LA VIA PR FE     C       EG +     L + +WY Y ++G ++FI Q+  PV+   ILS  +++ I K+K+FR       +  NN +     GD ++  +CL FF+ E+PSFFSK L++YL   N +I D  L  VAN+ +Y+DS+IN F+YI+SNP F+    QI
Sbjct:  237 INCSYPLETVGNKSDESDENLAAFALRIYNFHNYALLVIPIISIIFNGFCLFIFFDKIWKKSILSILLKYLSATEIVFNISGFL------YSVTKFIDYPSINQFSKLF--WVFILQIGTFSVASGLICRNWTILHLAIYRFDSICRPLNTKKIYTETSTYKLLWITYILAAVIAFPRTFEYKATFC-------EGNLVLKPSLLNYDWYAYGYQGSLIFIIQTAAPVVCTSILSISVMRAILKVKRFRKSFSKTIKTKNNAIS----GDALMIFICLAFFLFETPSFFSKALQSYLMRNNKIIEDVLLATVANLLVYLDSVINIFVYILSNPVFRKKLKQI 559          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000004763.1 (SMESG000004763.1)

HSP 1 Score: 95.9005 bits (237), Expect = 8.425e-22
Identity = 110/312 (35.26%), Postives = 158/312 (50.64%), Query Frame = 2
Query:   44 VINCSKIITNASGHVSYSNLLFTYYNYXXXXXXXXXXXXNTLAIFIFNHRLWRKS-LTSIILKYLSVFESLFNATVLI-----KNSYDNYAIQSKSPNFSSYESLLIDETFRLFIRLFGIVFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIEXXXXXXXXXXXXPRLFEKYVVACAEPLWKIEGKVAFLDS--SNWYVY--LHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFRNRNSN-NKVRPQPLGDKXXXXXXXXXXXXESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVD 946
            V NCS   TN SG+ S S  +   Y   SI L SI+   N  A+ IF+  LW    L + IL   S  E +F  T+L+         D Y I S+  N       ++D  FR  I LF + FLI RN  + +LA HR+EC+C P+  KR + S N+      I   A++IA PR+FE+    C +      G V  +    SN Y Y  ++  V +F+ Q G P+++V ILS  +L+ I++   FR  ++  N+ +     DK++  LC+ FF  E PSFFSK+        N  I+ H L  +ANI IY+D
Sbjct:   28 VTNCS---TN-SGNFSASANIEKIYQIISITLLSINSFANCFALHIFSKFLWHMPILMNQILARFSFIELIFQMTLLLFYLVEYIKIDEYIISSRFVNPG-----IMDALFRC-IYLFSVGFLIARNSWIILLAFHRFECICYPLRTKRYVTSANINFYFFGIVSFAILIAFPRMFEQTYTYCLQT-----GDVVNVSEALSNVYQYRIIYLNVFVFLIQIGIPLLSVNILSILMLRKIRQSLTFRRTSAAPNQKKKSFAADKLVVALCITFFTFEMPSFFSKLFHNSHIISNDKIIYH-LTFIANISIYID 323          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000045974.1 (SMESG000045974.1)

HSP 1 Score: 88.9669 bits (219), Expect = 1.321e-19
Identity = 77/252 (30.56%), Postives = 127/252 (50.40%), Query Frame = 2
Query:  206 SLTSIILKYLSVFESLFNATVLIKNSYDNYAIQSKSPNFSSYESLLIDETFRLFIRLFGI-VFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIEXXXXXXXXXXXXPRLFEKYVVACAEPLWKIEGKVAFLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFRN----RNSNNKVRPQPLGDKXXXXXXXXXXXXESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVD 946
            S+   I+  LS+ E  F  TV   N ++   + +    +   E   +D   R  I ++GI VFL+ RN  +  LA HRYE +C P+G+ + I       I +++   +L +  P++FE +++ C      +  ++  L+    Y  ++   ++F+ Q+ GPVI V  LS  IL  I +  +F      R SN+    +   D +I  LCL F +LE P+FFSK+ + +       IV   L ++ANICIY+D
Sbjct:   58 SMMMRIISNLSIIELFFQLTVFTFNLFEYCELDTIIIKYGYLEFYTVDIIVR--IHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLFLTFPKIFEDHIIYCHNT-GMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVIIVITLSILILIKIHRRTEFHRKYFVRKSNSLRHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQMFES-----IVIERLAMIANICIYLD 301          
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
G4VR075.139e-2835.44G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A3Q0KV195.334e-2835.44G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A183P9C97.755e-2835.28G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A193KUR78.601e-2834.81GCR101 OS=Schmidtea mediterranea OX=79327 GN=gcr10... [more]
A0A0R3SGW59.924e-2735.26G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000022454.10.000e+0100.00SMESG000022454.1[more]
SMESG000063294.11.054e-4943.33SMESG000063294.1[more]
SMESG000023730.12.318e-3133.92SMESG000023730.1[more]
SMESG000004763.18.425e-2235.26SMESG000004763.1[more]
SMESG000045974.11.321e-1930.56SMESG000045974.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003314 ID=SMED30003314|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1086bp
TACAAATTGTTTTAATATGGCCTAATTACAGATGTATATAAAAGTAATTA
ATTGTTCAAAGATAATTACAAATGCATCTGGTCATGTTTCATATTCTAAT
CTTTTATTCACATATTATAACTATTCGAGTATCGTTCTTTCATCAATAAG
TTTTCTTTTGAACACTCTTGCGATCTTTATCTTCAATCATCGGCTATGGA
GGAAATCTCTCACTAGTATTATATTGAAATATTTATCTGTGTTCGAATCT
CTATTCAATGCAACGGTTCTAATAAAAAATTCCTACGACAATTATGCAAT
TCAATCAAAGTCCCCAAATTTCAGCTCTTATGAATCTCTTTTAATAGATG
AAACATTTAGATTATTTATCCGTTTATTCGGAATCGTATTTCTTATTTGT
CGAAACGTAACGGTTGCTGTATTGGCTGCCCATCGGTATGAATGTGTCTG
TTTGCCAGTTGGAGCAAAAAGACGCATTAACAGTAGAAATGTCTTTCAAA
TAGAATTGGTGATATTTCTGCTAGCTTTAGTCATTGCCTTGCCAAGGTTG
TTTGAGAAGTACGTTGTCGCATGCGCCGAACCTCTTTGGAAAATAGAAGG
CAAAGTAGCATTTCTCGATTCAAGCAACTGGTATGTTTATCTTCACAAAG
GTGTTGTTTTATTCATTTTCCAAAGTGGTGGCCCAGTAATCACAGTTTTC
ATTCTCAGTTTCTACATTCTTCAAGTCATCAAAAAAATGAAAAAATTCCG
CAACCGGAATTCCAACAACAAAGTTCGTCCACAACCATTGGGAGACAAAA
TTATTTTCCCTCTTTGTTTAATATTTTTCATTCTTGAATCTCCGTCATTT
TTCAGTAAAATTCTCGAAGCGTATTTGCGATCAAAAAATCTCGTCATTGT
TGATCACACTTTGCTCGTCGTGGCAAACATTTGTATCTATGTTGATAGTA
TTATTAACTTTTTTATCTACATCATCTCTAATCCGACTTTTAAATTGGCT
TTTGTCCAGATTCTCAAACCTTGTTTCTTGGCTAAGTCGGAGAATGTTCA
TCAAACAACTGTAATTTTATGATAAATTTCCTTATA
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protein sequence of SMED30003314-orf-1

>SMED30003314-orf-1 ID=SMED30003314-orf-1|Name=SMED30003314-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=347bp
MYIKVINCSKIITNASGHVSYSNLLFTYYNYSSIVLSSISFLLNTLAIFI
FNHRLWRKSLTSIILKYLSVFESLFNATVLIKNSYDNYAIQSKSPNFSSY
ESLLIDETFRLFIRLFGIVFLICRNVTVAVLAAHRYECVCLPVGAKRRIN
SRNVFQIELVIFLLALVIALPRLFEKYVVACAEPLWKIEGKVAFLDSSNW
YVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFRNRNSNNKVRP
QPLGDKIIFPLCLIFFILESPSFFSKILEAYLRSKNLVIVDHTLLVVANI
CIYVDSIINFFIYIISNPTFKLAFVQILKPCFLAKSENVHQTTVIL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
IPR000299FERM_domain
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0005856cytoskeleton
GO:0016020membrane
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 15..339
e-value: 2.3E-29
score: 104.3
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 23..326
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 28..324
e-value: 1.23227E-22
score: 94.2368
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 23..337
NoneNo IPR availableTMHMMTMhelixcoord: 29..51
NoneNo IPR availableTMHMMTMhelixcoord: 208..230
NoneNo IPR availableTMHMMTMhelixcoord: 257..274
NoneNo IPR availableTMHMMTMhelixcoord: 154..171
NoneNo IPR availableTMHMMTMhelixcoord: 111..133
NoneNo IPR availableTMHMMTMhelixcoord: 294..316
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 115..313
e-value: 1.1E-7
score: 31.4
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 43..313
score: 15.758