Rotatin
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30003301 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of Rotatin vs. Ensembl Human
Match: RTTN (rotatin [Source:HGNC Symbol;Acc:HGNC:18654]) HSP 1 Score: 93.5893 bits (231), Expect = 4.333e-18 Identity = 218/1036 (21.04%), Postives = 412/1036 (39.77%), Query Frame = 2
Query: 1301 IYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFLN-NNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLI-----SSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVND---NKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKI-LDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRV-GKYSANKTSKTEWTFSNFSKIPNQKWN--YQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETSL-WISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMT--TPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYP-FDNLSKLLIHIHLMSRSLELKSNF--FQG-NTLKVISNVINLCG-------DLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWL--------------RKSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSM 4285
I + LFA+ + ++ E A ++L E T L D + L ++ Y+E + + K + + S E F + E K + + A+ +L Y KE S I +S + G + + + +K+ + C +I + L + KP S+ N I+FLL + + +FG+ E++ A A+ L +G+ +M+ + W + + + LQ D +G IL L ++S + L L K VR A + L L + + + + + ++S V D K RL + + + ++ +K+++I+ S ++D+ +RKSAAEQL + +Q MH + ++E L + D + LV + +L +++ +R +L S L+ L +++L F D V ++ ++ L++F+ V + D S + LP V +Y S +S + + + LRI W L + G +N L K++ +++D L L+ D+ L + L D LH I A H +V++++ + L+ + SL W + ++FL +P+C+ D LL I+ L +IK N + + W+++++ + N L + + +TD R T + +++ L F L+ LN ++++ ++ S+++ + + + L Q + + P F L L R+L +N F G ++ ++ +++C ++I F+ R ++++FMG GV + L+ HL E+ + + W + WL+ L R ++R S + S + E L C I LW + + LD SE +VR EA ILQNLL +P P +
Sbjct: 474 ISISLFAV--RLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCC-------LEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASS---------DTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDAR--VLSRVTDLFIGKKPIEL-RLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLL--------KQMNSETKTQEILD-------ALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIR-TAVRQQLQKELIALFDTLL----LNFMEVTDRKC---SELLYVFQTQLALKLLQCLKVTDAPHFYGLPSL-------ERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLL-----HLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNI-SGGLWGTVVNILLDQSECSMVRREAAFILQNLL-VIPMPTEI 1451 HSP 2 Score: 59.3066 bits (142), Expect = 1.271e-7 Identity = 83/346 (23.99%), Postives = 159/346 (45.95%), Query Frame = 2
Query: 5375 NTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTV-KLIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNG-----SGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANL 6391
N L LL S+ + + ++ I++ ++K+++ AQ N+D +R K ++ + +IKE+ ++LL+N Y++ + A+ +++ LW L+D L+ L L +A+ N ++ S SL+ I +L S L TV +++F+LLSNL + ++ + FL F + P K S + I L L++++S E+GQ I++ +G + + K + + +L LLI N+C P K K++ N +I + CL+S + +++ + L AL+Y+ K + LKS ++
Sbjct: 1864 NALMSLLAVSRRAQKHALKANLIDNCMEQMKHIN------AQLNLD-SLRPGKAALKKKED-----GVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLAS----QMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILW--LKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLL-----PLLIFHNVCFSPANKPKILAN-EKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSV 2185 HSP 3 Score: 56.9954 bits (136), Expect = 5.583e-7 Identity = 42/123 (34.15%), Postives = 66/123 (53.66%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNT-SNFQI-LDQIIKNLLKVNSNDTILS 411
L + KL H L E+R RALK+I K+E +I DL + ++ +LLEWFN V + VL L+ ++ + ++ +G +EFL++L+ SN N Q +D I+ L + S LS
Sbjct: 3 LAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLR--SNVEPNLQAEIDGILDGLFLLPSEVPALS 123 HSP 4 Score: 55.8398 bits (133), Expect = 1.282e-6 Identity = 38/119 (31.93%), Postives = 67/119 (56.30%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQN 931
LK S P +PLT D++VLS + +LRS + ++ + + DVI++DFP+ +FLQR +++ LL + + + + +V + +LC + +N DP FFS K+ ++QN
Sbjct: 170 LKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKH--DTVSQN 286
BLAST of Rotatin vs. Ensembl Human
Match: RTTN (rotatin [Source:HGNC Symbol;Acc:HGNC:18654]) HSP 1 Score: 93.2041 bits (230), Expect = 6.289e-18 Identity = 218/1036 (21.04%), Postives = 412/1036 (39.77%), Query Frame = 2
Query: 1301 IYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFLN-NNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLI-----SSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVND---NKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKI-LDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRV-GKYSANKTSKTEWTFSNFSKIPNQKWN--YQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETSL-WISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMT--TPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYP-FDNLSKLLIHIHLMSRSLELKSNF--FQG-NTLKVISNVINLCG-------DLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWL--------------RKSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSM 4285
I + LFA+ + ++ E A ++L E T L D + L ++ Y+E + + K + + S E F + E K + + A+ +L Y KE S I +S + G + + + +K+ + C +I + L + KP S+ N I+FLL + + +FG+ E++ A A+ L +G+ +M+ + W + + + LQ D +G IL L ++S + L L K VR A + L L + + + + + ++S V D K RL + + + ++ +K+++I+ S ++D+ +RKSAAEQL + +Q MH + ++E L + D + LV + +L +++ +R +L S L+ L +++L F D V ++ ++ L++F+ V + D S + LP V +Y S +S + + + LRI W L + G +N L K++ +++D L L+ D+ L + L D LH I A H +V++++ + L+ + SL W + ++FL +P+C+ D LL I+ L +IK N + + W+++++ + N L + + +TD R T + +++ L F L+ LN ++++ ++ S+++ + + + L Q + + P F L L R+L +N F G ++ ++ +++C ++I F+ R ++++FMG GV + L+ HL E+ + + W + WL+ L R ++R S + S + E L C I LW + + LD SE +VR EA ILQNLL +P P +
Sbjct: 474 ISISLFAV--RLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCC-------LEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASS---------DTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDAR--VLSRVTDLFIGKKPIEL-RLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLL--------KQMNSETKTQEILD-------ALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIR-TAVRQQLQKELIALFDTLL----LNFMEVTDRKC---SELLYVFQTQLALKLLQCLKVTDAPHFYGLPSL-------ERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLL-----HLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNI-SGGLWGTVVNILLDQSECSMVRREAAFILQNLL-VIPMPTEI 1451 HSP 2 Score: 59.6918 bits (143), Expect = 9.021e-8 Identity = 93/386 (24.09%), Postives = 177/386 (45.85%), Query Frame = 2
Query: 5375 NTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTV-KLIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNG-----SGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANL---VTKLMSL-REIYENDSKNGL-------LLDIISLLNA 6478
N L LL S+ + + ++ I++ ++K+++ AQ N+D +R K ++ + +IKE+ ++LL+N Y++ + A+ +++ LW L+D L+ L L +A+ N ++ S SL+ I +L S L TV +++F+LLSNL + ++ + FL F + P K S + I L L++++S E+GQ I++ +G + + K + + +L LLI N+C P K K++ N +I + CL+S + +++ + L AL+Y+ K + LKS ++ V + SL ++ + N N L L +++ LLN+
Sbjct: 1864 NALMSLLAVSRRAQKHALKANLIDNCMEQMKHIN------AQLNLD-SLRPGKAALKKKED-----GVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLAS----QMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILW--LKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLL-----PLLIFHNVCFSPANKPKILAN-EKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNS 2225 HSP 3 Score: 56.9954 bits (136), Expect = 5.685e-7 Identity = 42/123 (34.15%), Postives = 66/123 (53.66%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNT-SNFQI-LDQIIKNLLKVNSNDTILS 411
L + KL H L E+R RALK+I K+E +I DL + ++ +LLEWFN V + VL L+ ++ + ++ +G +EFL++L+ SN N Q +D I+ L + S LS
Sbjct: 3 LAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLR--SNVEPNLQAEIDGILDGLFLLPSEVPALS 123 HSP 4 Score: 55.8398 bits (133), Expect = 1.350e-6 Identity = 38/119 (31.93%), Postives = 67/119 (56.30%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQN 931
LK S P +PLT D++VLS + +LRS + ++ + + DVI++DFP+ +FLQR +++ LL + + + + +V + +LC + +N DP FFS K+ ++QN
Sbjct: 170 LKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKH--DTVSQN 286
BLAST of Rotatin vs. Ensembl Human
Match: RTTN (rotatin [Source:HGNC Symbol;Acc:HGNC:18654]) HSP 1 Score: 72.0182 bits (175), Expect = 1.508e-11 Identity = 106/508 (20.87%), Postives = 206/508 (40.55%), Query Frame = 2
Query: 1301 IYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFLN-NNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLI-----SSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVND---NKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKI-LDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQD 2794
I + LFA+ + ++ E A ++L E T L D + L ++ Y+E + + K + + S E F + E K + + A+ +L Y KE S I +S + G + + + +K+ + C +I + L + KP S+ N I+FLL + + +FG+ E++ A A+ L +G+ +M+ + W + + + LQ D +G IL L ++S + L L K VR A + L L + + + + + ++S V D K RL + + + ++ +K+++I+ S ++D+ +RKSAAEQL + +Q MH + ++E L + D + LV + +L +++ +R +L S L+ L +++L F D V ++ ++ L++F+ V + D
Sbjct: 301 ISISLFAV--RLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCC-------LEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASS---------DTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDAR--VLSRVTDLFIGKKPIEL-RLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMD 787
BLAST of Rotatin vs. Ensembl Human
Match: RTTN (rotatin [Source:HGNC Symbol;Acc:HGNC:18654]) HSP 1 Score: 58.151 bits (139), Expect = 1.064e-7 Identity = 92/386 (23.83%), Postives = 178/386 (46.11%), Query Frame = 2
Query: 5375 NTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTV-KLIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNG-----SGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANL---VTKLMSL-REIYENDSKNGL-------LLDIISLLNA 6478
N L LL S+ + + ++ I++ ++K+++++L N+D +R K ++ + +IKE+ ++LL+N Y++ + A+ +++ LW L+D L+ L L +A+ N ++ S SL+ I +L S L TV +++F+LLSNL + ++ + FL F + P K S + I L L++++S E+GQ I++ +G + + K + + +L LLI N+C P K K++ N +I + CL+S + +++ + L AL+Y+ K + LKS ++ V + SL ++ + N N L L +++ LLN+
Sbjct: 33 NALMSLLAVSRRAQKHALKANLIDNCMEQMKHINAQL------NLDS-LRPGKAALKKKED-----GVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLAS----QMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILW--LKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLL-----PLLIFHNVCFSPANKPKILAN-EKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNS 394
BLAST of Rotatin vs. Ensembl Human
Match: RTTN (rotatin [Source:HGNC Symbol;Acc:HGNC:18654]) HSP 1 Score: 56.9954 bits (136), Expect = 3.149e-7 Identity = 43/123 (34.96%), Postives = 65/123 (52.85%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNT-SNFQI-LDQIIKNLLKVNSNDTILS 411
L + KL H L E+R RALK+I K+E +I DL + ++ +LLEWFN V + VL L+ ++ + ++ +G +EFL+ KL SN N Q +D I+ L + S LS
Sbjct: 3 LAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLS--KLRSNVEPNLQAEIDGILDGLFLLPSEVPALS 123 HSP 2 Score: 55.4546 bits (132), Expect = 9.695e-7 Identity = 38/119 (31.93%), Postives = 67/119 (56.30%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQN 931
LK S P +PLT D++VLS + +LRS + ++ + + DVI++DFP+ +FLQR +++ LL + + + + +V + +LC + +N DP FFS K+ ++QN
Sbjct: 170 LKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKH--DTVSQN 286
BLAST of Rotatin vs. Ensembl Zebrafish
Match: rttn (rotatin [Source:ZFIN;Acc:ZDB-GENE-090609-5]) HSP 1 Score: 107.457 bits (267), Expect = 1.849e-22 Identity = 197/950 (20.74%), Postives = 373/950 (39.26%), Query Frame = 2
Query: 1793 FTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVNDNKHERWFPRLLNGQSKSLDI------------DNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYF----ISNGGLHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEI---------KILEIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETS---------LWISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTN-HMKDFIQWLVQIMTTPNNSLFYFLEKGD-------KLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYP-FDNLSKLLIHIHLMSRSLELKSNFFQGNT--LKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWL------RKS------------PWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSMEIPIDVNQNDENEDISSLFIDRNSSGKYQEINGFYQKLLYLDFFRKAGLSLTNFYT 4453
++ I++ L ++ AKP S+ ++FLL + + + +FGL ++ E+ A + L +G+ +M+ WR+ + + LQ +G + +K R+ + L L + K + VRK A Q+L L QN L D+ P L N +KS+DI ++ K+++I S +D+ +RKSAAEQL I LQ +MH +S+ ++ E + + + L+ S+ +L ++V +R +L + + L++ +L ++ V++ ++++ +++F+ + + D + E + I R LPF+ + A S +S + + K ++ L+I W H G++ L + F D L L+ES VL S + + D +H + +A +H V I R H +L D + + T W S +FL+ P+C D LL I+ L K + + +D L ++ +++L L D + G+ D + L+ ++++ L F L+ + + ++ S + + + +++ + L+ + P + + + H+ + + + NT LK +S ++ ++I F+ +S++FMG GV + L+ HL E+ + E+T DW+ R+ WL+ L R ++R S + + + E+ L C I LW+ L + L+ E +VR EA ILQNLL M +P + + E S D +S + L + FF G + + YT
Sbjct: 635 YSCTLSILKECLGVQNVAKPASSICAGVHFLLHSRVLYEISVFGLQDQATEVSAAAKDILLYLLKGRLMMTAASWRRFNEALYPVIPILQSYAGTEDALGN--------CILLLSEASAEKGDEMRSPTARLRAALRLLFTKQHTVRKMAVQHLLPHLTSAE------GAQNSRPTL-----DSSTLSCLPNLYN-LNKSVDIKFSSSDKSYVKVESVNKLYNILASETVDLVLRKSAAEQLAIVLQDSSMHAVLKALGLSDKLTSFITECVNSNV---PSMDCLLESSACMLRKLVFADPSLRHSLAQQTTFLITLLRASLIIKENQGDVSEAAALMCILLFDEIARMDVWTDEAGSDTGLSSFSLPAAIIRRYNLPFQAPSHHA--VSPYCSVLPPYSDLLSLKPAWEA-----LQITWNTAWHSGIDGLLEHLRGFRSDVPEFHSD--------------LVLSESQAVVLRISNMRSGIEDCIHTVCDASSHSTV--------TFAITRMHLYLLFDFLALKSTTQSCKNILQTLSWHSAVERFLVVRPACHQDERLLVDILSFLNTFFKQNQSELDQQDLKWLLELLLNQESHTLLNLLLSADPQNHTHVQSQGEVDKLQAHLS-HRLQRELTGLFNTLLLLMTFTSDRMCEVLSGPFASELSL-RLLQCLRLSDAPCFYGLPSLERTLRTMAHVTALP-GWSTHTPSVEPNTLCLKYLSGLL----EVISSFYVDLGGNSMSFMGKGVTKNAVICLL-----HLSHEM--MKEST--NKDWISLWSLGREQSADEQSAAQLGLAWLIPLWVDRDPEVRCASLAVGAALSSLERGCVALSASCQNI-SGGLWATVLNILLEKQECSMVRREAAFILQNLL-------VMPMPANAEEAKECAWQSPCVHDEDSGLCLVGLPALQALLYHCQFFESVGQMVKSCYT 1508 HSP 2 Score: 70.0922 bits (170), Expect = 3.467e-11 Identity = 41/113 (36.28%), Postives = 66/113 (58.41%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNLLKV 387
L+ + K+ H L E+R RALKNI +K++ +I++ DL + K +LLEWFN V + VL+LV+M S+ G + + ++G +EFLTQL + I+D + L +
Sbjct: 3 LSSLIKKIGHSLVEIRVRALKNIRSKLDHGLISVPDLVQEKMLFVFLLEWFNFPEVPMQEEVLELVDMLSKHPTGAQMLREVGAVEFLTQLSPNVDLRLRTIVDGVFDQLFHL 115 HSP 3 Score: 64.6994 bits (156), Expect = 1.600e-9 Identity = 79/349 (22.64%), Postives = 152/349 (43.55%), Query Frame = 2
Query: 5369 VHNTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTV--KLIFLLLSNLVWCPEGINILLRIRFLDKFEQINPNQLQKSASGIAIQNLWLGLI--ISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKL 6403
V L LL ++Y + G I+S +K+++++L ++ + N ++ + ++E+ +LE+L+N Y+ ++VA + +++ W LLD + L L +A+ A+ S + + N L + +N+ L F LL+NL +G +LL+ FL F + + A++ L L L +++SF E+GQ I++ G+ + V Q+ + + LLI+ N+C K K++ N + +E + CL S+ I+ + S L ALL++N K + LK ++ K+
Sbjct: 1863 VAKALMSLLACCSSAQSYACKAGVIDSCVERMKHIYAELH-----------LESVKTGRNAHRRKQVENSMRELKMILEMLRNCLYQHNECKAVATDLRLAVVLLAFWPWLLLDDLTMAAALELLCVYTANCTAACSALCFSSSSSSVSVGSVQPVLPKTAVTNSLMHAVMKLASQVAPDNSSIHSLAFSLLANLAISRDGKGLLLKCNFLQNFLSLPMPKASGRGGAPAVETLSLWLRLLLNVSFGEDGQQMILRLRGALELLVELTQSKHSSAKPTVLLILHNICFCSANKPKVLANDKA-VELLVSCLSSSSSDIRNIGASALWALLHNNQKAKATLKCPSIRLKV 2199 HSP 4 Score: 64.3142 bits (155), Expect = 2.077e-9 Identity = 37/111 (33.33%), Postives = 61/111 (54.95%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI-----SEKQYSTI-AVKAISKLCYKMLQYINTCLDPNFFSTKN 907
LK SV P + L D+++LS + +LRSD+ +V + + DVI++DFP+ +FLQR ++ LL + + Y T A+ + +LC + + DP+FFS K
Sbjct: 166 LKFSVFPWLSLNTTDRHILSSNESSLRSDNHNLVRTTCELLRDVIMQDFPAEIFLQRPSTVQSLLSLLGLNVDGDVSYLTFQALACLQQLCRNLKSRLRFYRDPSFFSAKQ 276
BLAST of Rotatin vs. Ensembl Zebrafish
Match: rttn (rotatin [Source:ZFIN;Acc:ZDB-GENE-090609-5]) HSP 1 Score: 103.605 bits (257), Expect = 2.540e-21 Identity = 198/974 (20.33%), Postives = 380/974 (39.01%), Query Frame = 2
Query: 1793 FTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRF------------DRLWNCQLNIQNM---TDFLVSSVNDN-------KHERWFPRLL-----------NGQSKSL---DIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYF----ISNGGLHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEI---------KILEIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETS---------LWISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTN-HMKDFIQWLVQIMTTPNNSLFYFLEKGD-------KLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYP-FDNLSKLLIHIHLMSRSLELKSNFFQGNT--LKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWL------RKS------------PWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSMEIPIDVNQNDENEDISSLFIDRNSSGKYQEINGFYQKLLYLDFFRKAGLSLTNFYT 4453
++ I++ L ++ AKP S+ ++FLL + + + +FGL ++ E+ A + L +G+ +M+ WR+ + + LQ +G + +K R+ + L L + K + VRK A Q+L L +C N+ N+ D SS + + H F +LL N +S+ +++ K+++I S +D+ +RKSAAEQL I LQ +MH +S+ ++ E + + + L+ S+ +L ++V +R +L + + L++ +L ++ V++ ++++ +++F+ + + D + E + I R LPF+ + A S +S + + K ++ L+I W H G++ L + F D L L+ES VL S + + D +H + +A +H V I R H +L D + + T W S +FL+ P+C D LL I+ L K + + +D L ++ +++L L D + G+ D + L+ ++++ L F L+ + + ++ S + + + +++ + L+ + P + + + H+ + + + NT LK +S ++ ++I F+ +S++FMG GV + L+ HL E+ + E+T DW+ R+ WL+ L R ++R S + + + E+ L C I LW+ L + L+ E +VR EA ILQNLL M +P + + E S D +S + L + FF G + + YT
Sbjct: 635 YSCTLSILKECLGVQNVAKPASSICAGVHFLLHSRVLYEISVFGLQDQATEVSAAAKDILLYLLKGRLMMTAASWRRFNEALYPVIPILQSYAGTEDALGN--------CILLLSEASAEKGDEMRSPTARLRAALRLLFTKQHTVRKMAVQHLLPHLTSAEGAQNSRPTLDSSTLSCLPNLYNLNKSVDIKFSSSDKSYVKVKHFSHSAAFDKLLSRITLNQLVYANSDKRSVVFKQVESVNKLYNILASETVDLVLRKSAAEQLAIVLQDSSMHAVLKALGLSDKLTSFITECVNSNV---PSMDCLLESSACMLRKLVFADPSLRHSLAQQTTFLITLLRASLIIKENQGDVSEAAALMCILLFDEIARMDVWTDEAGSDTGLSSFSLPAAIIRRYNLPFQAPSHHA--VSPYCSVLPPYSDLLSLKPAWEA-----LQITWNTAWHSGIDGLLEHLRGFRSDVPEFHSD--------------LVLSESQAVVLRISNMRSGIEDCIHTVCDASSHSTV--------TFAITRMHLYLLFDFLALKSTTQSCKNILQTLSWHSAVERFLVVRPACHQDERLLVDILSFLNTFFKQNQSELDQQDLKWLLELLLNQESHTLLNLLLSADPQNHTHVQSQGEVDKLQAHLS-HRLQRELTGLFNTLLLLMTFTSDRMCEVLSGPFASELSL-RLLQCLRLSDAPCFYGLPSLERTLRTMAHVTALP-GWSTHTPSVEPNTLCLKYLSGLL----EVISSFYVDLGGNSMSFMGKGVTKNAVICLL-----HLSHEM--MKEST--NKDWISLWSLGREQSADEQSAAQLGLAWLIPLWVDRDPEVRCASLAVGAALSSLERGCVALSASCQNI-SGGLWATVLNILLEKQECSMVRREAAFILQNLL-------VMPMPANAEEAKECAWQSPCVHDEDSGLCLVGLPALQALLYHCQFFESVGQMVKSCYT 1544 HSP 2 Score: 70.0922 bits (170), Expect = 4.077e-11 Identity = 41/113 (36.28%), Postives = 66/113 (58.41%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNLLKV 387
L+ + K+ H L E+R RALKNI +K++ +I++ DL + K +LLEWFN V + VL+LV+M S+ G + + ++G +EFLTQL + I+D + L +
Sbjct: 3 LSSLIKKIGHSLVEIRVRALKNIRSKLDHGLISVPDLVQEKMLFVFLLEWFNFPEVPMQEEVLELVDMLSKHPTGAQMLREVGAVEFLTQLSPNVDLRLRTIVDGVFDQLFHL 115 HSP 3 Score: 64.6994 bits (156), Expect = 1.730e-9 Identity = 79/349 (22.64%), Postives = 152/349 (43.55%), Query Frame = 2
Query: 5369 VHNTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTV--KLIFLLLSNLVWCPEGINILLRIRFLDKFEQINPNQLQKSASGIAIQNLWLGLI--ISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKL 6403
V L LL ++Y + G I+S +K+++++L ++ + N ++ + ++E+ +LE+L+N Y+ ++VA + +++ W LLD + L L +A+ A+ S + + N L + +N+ L F LL+NL +G +LL+ FL F + + A++ L L L +++SF E+GQ I++ G+ + V Q+ + + LLI+ N+C K K++ N + +E + CL S+ I+ + S L ALL++N K + LK ++ K+
Sbjct: 1899 VAKALMSLLACCSSAQSYACKAGVIDSCVERMKHIYAELH-----------LESVKTGRNAHRRKQVENSMRELKMILEMLRNCLYQHNECKAVATDLRLAVVLLAFWPWLLLDDLTMAAALELLCVYTANCTAACSALCFSSSSSSVSVGSVQPVLPKTAVTNSLMHAVMKLASQVAPDNSSIHSLAFSLLANLAISRDGKGLLLKCNFLQNFLSLPMPKASGRGGAPAVETLSLWLRLLLNVSFGEDGQQMILRLRGALELLVELTQSKHSSAKPTVLLILHNICFCSANKPKVLANDKA-VELLVSCLSSSSSDIRNIGASALWALLHNNQKAKATLKCPSIRLKV 2235 HSP 4 Score: 64.3142 bits (155), Expect = 2.189e-9 Identity = 37/111 (33.33%), Postives = 61/111 (54.95%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI-----SEKQYSTI-AVKAISKLCYKMLQYINTCLDPNFFSTKN 907
LK SV P + L D+++LS + +LRSD+ +V + + DVI++DFP+ +FLQR ++ LL + + Y T A+ + +LC + + DP+FFS K
Sbjct: 166 LKFSVFPWLSLNTTDRHILSSNESSLRSDNHNLVRTTCELLRDVIMQDFPAEIFLQRPSTVQSLLSLLGLNVDGDVSYLTFQALACLQQLCRNLKSRLRFYRDPSFFSAKQ 276
BLAST of Rotatin vs. Ensembl Xenopus
Match: RTTN (rotatin [Source:NCBI gene;Acc:100379860]) HSP 1 Score: 91.2781 bits (225), Expect = 1.835e-17 Identity = 202/1033 (19.55%), Postives = 388/1033 (37.56%), Query Frame = 2
Query: 1283 LSNKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFL-NNNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFIKCDE---ILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVNDNKHERWFPRLLNGQSKSLDI-DNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKI-LDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLN----VDIYDIFNETSL----WISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNS-LFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQG-----NTLKVISNVINLCG----------------------DLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLR------------------KSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLD 4201
L + R ++ + + SI E A L E L D + +I+++E + + K+S+ S E F L + E K + + AE +L L + ++F S +IF + + + +E +L LM + + +IV++SL I KP S+ I+FLL + + + FGL EI A + L +G+ +M W ++ + LQ D +G IL L +++++ I L ++K VR A +++ L + C+ Q + L ++V E+ L+ KS + D +K+ +I+LS +D +++KSAAEQ+ + Q MH+ G + +L L + D ++ +++L +IV R L + L +++L + + + +++L +++F+ + + D + ++ ++ R LP V Y A + S W + LR+ W + H G++ +L K + ++ +EI ++ L L+ ++ L + L D L I A++H +V+S++ + N LN ++ +SL W + +FLL P+C D LL ++ L I++ N +QW+++++ + S LF +E+ + K + ++ L FF LM +CL +T + + +HL++R + F G TL+ +S+V + G ++I F+ +++++MG GV + L+ HL E+ + TDW+ WL+ L R ++R S I S + ++ L C I LW +++L+
Sbjct: 455 LMSHRMAFISISLFTMRLLQSILPVEKANDVLPESLMNALFLISLDLPFRMAYPSVHESVISFMEQLNSEHCGIYKQSSETAYSIECATTFMLYSRKEGKKDFIELVELAEKALSSLPFHKHFPLVQKFIQICS-----DIFKSAQSSPVLLEESQKVLLCLMSHWMPLVKAEAYKTNLEIVKDSLGIHNVTKPVSSVCQGIHFLLHSKVLYEISTFGLQESNSEICTAAKTILSYLLQGRLVMENHTWNTLIESICPVIPVLQGFADTEETLGTSILTLCETSANDDGILPKTA---------RIRAALRQLFSKKQAVRSVAIKWIAVHLMNEEDSTCK-RPQILGSTLSNAVCLFILEQPLDLKLDNNEKSFFMEDTVQKVCEIFLSDTVDFALQKSAAEQMSVIAQDVKMHSVLKKPGVVDKILHYLYKYVQQDGKAFECMILPCLMLLRKIVYADPGFRLTLSQKPCFLLTLFRVSLMMQQEVTSLTEAAALLCMLLFDEISRMD--TCIFYVIYVLFLRFHLPVLVSAYHAVSPYTMASSLS-------ADWLTSKPVSVMLRMSWNISWHQGVDPFL---------KTVANEQ-----EEIENSLETLKLSPEEVLTLKMTHSVSGLQDCLSSIIQAVSHGEVRSAVARM-------NFYLLNDRLVLNCLTACYMSSLKCLPWHTALSRFLLVHPACEEDEKLLADVIRFLNKILQEQKGNSDPHDLQWILEMLLKQSPSPLFELMEQKEYPAKKDLDDTRAAVRQHLQKELMMFFNTLM------------------------------LCLTSVTDRKCLVLGGTFRTQLALHLLNRLKISDAPHFYGLPSLAQTLRGMSHVTSQPGWSAYSPSTEPFAICVKTLTSLLEIISSFYVEWGGNALSYMGKGVTKSTVLCLL-----HLSHEMTAQAKN----TDWIHLWTLPYDHGTEDQVLSRLGLEWLIPLWVDRDPEVRFTSLGIASALTCLQKGCLALSASCQNI-SGGLWGTVFSIFLN 1402 HSP 2 Score: 72.7886 bits (177), Expect = 8.963e-12 Identity = 41/135 (30.37%), Postives = 71/135 (52.59%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI-------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQNYGEYEEDNHWNSDDL 976
LK S P +PLT DK+VLS + +LRS++ +V Q DV+++DFP +FLQR +++ LL ++ ++ ++ +S+LC + + DPNF STK + + ++ Y D N+ +
Sbjct: 163 LKFSTFPWLPLTTTDKHVLSSNESSLRSNNPSLVWRSCQLLQDVVMQDFPPEIFLQRPKIVQSLLSLFGLASEKDGQQHLASQSISCLSQLCTLLRNRFSFHRDPNFLSTKQEI-VSHNSFASYCHDAQANNSQV 296 HSP 3 Score: 67.781 bits (164), Expect = 2.867e-10 Identity = 90/328 (27.44%), Postives = 158/328 (48.17%), Query Frame = 2
Query: 5387 ILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTVK-LIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKI 6364
ILLG S +V G I+ EIK++H++L N+D +R K + + + +K++ ++++L+N Y++ ++ A+ F +++ LW L+D L+ L L +A ++ + PP G+S SL+ NI +L S D TA +TV+ + F LLSNLV + I+ + FL F ++ P KS +A N WL + +LS +GQ I+K GS + V ++ LLI+ N+C + K K++ N + + + CL+S+++ +Q+ S ALL+ K+
Sbjct: 1594 ILLGKSHLVD------GCIQ----EIKHIHAQL------NLD-SLRPGKAAHKKKEE-----NHLKQLKVLMQILRNCLYQNEEGKAAAIDSQFPFVLHSLWPWLLMDDALMQGALQLLCVYTAYYPAACTSLCWTNTGVTTPQPPPKTGASNSLMHNILKLAS-DSTA---NNSTVQQMAFALLSNLVVIQDSKCIIQKSNFLQNFLTLSLPKAGTKSLCILA--NNWLKFLWNLSMGSDGQQMILKLKGSLDLLVELTTYKQKMISPVALLILHNVCFNTANKAKILSNDKA-VAVLSTCLESDNLLDQQIGCSAFWALLHGYQKV 1892 HSP 4 Score: 54.299 bits (129), Expect = 3.330e-6 Identity = 36/110 (32.73%), Postives = 60/110 (54.55%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNL 378
++ V KL H L E+R RALKNI K++ ++++ DL + K F +LLEWFN V VL L+ + + +I++G ++F +QL+ ++D I+ L
Sbjct: 3 ISTLVKKLGHQLVEIRERALKNILCKLDHNLVSLVDLAQEKTFFLHLLEWFNFPAVPMKADVLHLLNKLVKHSEAAQWLIQLGAVDFFSQLRSNVEPDLQAVVDSILDGL 112
BLAST of Rotatin vs. Ensembl Xenopus
Match: RTTN (rotatin [Source:NCBI gene;Acc:100379860]) HSP 1 Score: 91.2781 bits (225), Expect = 2.143e-17 Identity = 203/1036 (19.59%), Postives = 390/1036 (37.64%), Query Frame = 2
Query: 1283 LSNKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFL-NNNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFIKCDE---ILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVNDNKHERWFPRLLNGQSKSLDI-DNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGG----LHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLN----VDIYDIFNETSL----WISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNS-LFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQG-----NTLKVISNVINLCG----------------------DLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLR------------------KSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLD 4201
L + R ++ + + SI E A L E L D + +I+++E + + K+S+ S E F L + E K + + AE +L L + ++F S +IF + + + +E +L LM + + +IV++SL I KP S+ I+FLL + + + FGL EI A + L +G+ +M W ++ + LQ D +G IL L +++++ I L ++K VR A +++ L + C+ Q + L ++V E+ L+ KS + D +K+ +I+LS +D +++KSAAEQ+ + Q MH+ G LHY+ + ++ D ++ +++L +IV R L + L +++L + + + +++L +++F+ + + D + ++ ++ R LP V Y A + S W + LR+ W + H G++ +L K + ++ +EI ++ L L+ ++ L + L D L I A++H +V+S++ + N LN ++ +SL W + +FLL P+C D LL ++ L I++ N +QW+++++ + S LF +E+ + K + ++ L FF LM +CL +T + + +HL++R + F G TL+ +S+V + G ++I F+ +++++MG GV + L+ HL E+ + TDW+ WL+ L R ++R S I S + ++ L C I LW +++L+
Sbjct: 440 LMSHRMAFISISLFTMRLLQSILPVEKANDVLPESLMNALFLISLDLPFRMAYPSVHESVISFMEQLNSEHCGIYKQSSETAYSIECATTFMLYSRKEGKKDFIELVELAEKALSSLPFHKHFPLVQKFIQICS-----DIFKSAQSSPVLLEESQKVLLCLMSHWMPLVKAEAYKTNLEIVKDSLGIHNVTKPVSSVCQGIHFLLHSKVLYEISTFGLQESNSEICTAAKTILSYLLQGRLVMENHTWNTLIESICPVIPVLQGFADTEETLGTSILTLCETSANDDGILPKTA---------RIRAALRQLFSKKQAVRSVAIKWIAVHLMNEEDSTCK-RPQILGSTLSNAVCLFILEQPLDLKLDNNEKSFFMEDTVQKVCEIFLSDTVDFALQKSAAEQMSVIAQDVKMHSVLKKPGVVDKILHYLYKYVQQ---DGKAFECMILPCLMLLRKIVYADPGFRLTLSQKPCFLLTLFRVSLMMQQEVTSLTEAAALLCMLLFDEISRMD--TCIFYVIYVLFLRFHLPVLVSAYHAVSPYTMASSLS-------ADWLTSKPVSVMLRMSWNISWHQGVDPFL---------KTVANEQ-----EEIENSLETLKLSPEEVLTLKMTHSVSGLQDCLSSIIQAVSHGEVRSAVARM-------NFYLLNDRLVLNCLTACYMSSLKCLPWHTALSRFLLVHPACEEDEKLLADVIRFLNKILQEQKGNSDPHDLQWILEMLLKQSPSPLFELMEQKEYPAKKDLDDTRAAVRQHLQKELMMFFNTLM------------------------------LCLTSVTDRKCLVLGGTFRTQLALHLLNRLKISDAPHFYGLPSLAQTLRGMSHVTSQPGWSAYSPSTEPFAICVKTLTSLLEIISSFYVEWGGNALSYMGKGVTKSTVLCLL-----HLSHEMTAQAKN----TDWIHLWTLPYDHGTEDQVLSRLGLEWLIPLWVDRDPEVRFTSLGIASALTCLQKGCLALSASCQNI-SGGLWGTVFSIFLN 1387 HSP 2 Score: 72.7886 bits (177), Expect = 8.352e-12 Identity = 41/135 (30.37%), Postives = 71/135 (52.59%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI-------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQNYGEYEEDNHWNSDDL 976
LK S P +PLT DK+VLS + +LRS++ +V Q DV+++DFP +FLQR +++ LL ++ ++ ++ +S+LC + + DPNF STK + + ++ Y D N+ +
Sbjct: 148 LKFSTFPWLPLTTTDKHVLSSNESSLRSNNPSLVWRSCQLLQDVVMQDFPPEIFLQRPKIVQSLLSLFGLASEKDGQQHLASQSISCLSQLCTLLRNRFSFHRDPNFLSTKQEI-VSHNSFASYCHDAQANNSQV 281 HSP 3 Score: 69.3218 bits (168), Expect = 9.905e-11 Identity = 92/366 (25.14%), Postives = 174/366 (47.54%), Query Frame = 2
Query: 5273 FTELLLKKRESLSRINAKSHSYTDTSINERINVHNTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTVK-LIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKI 6364
F+ L +K ++ + + +H Y S +++ ++ T K L +L+ ++ EIK++H++L N+D +R K + + + +K++ ++++L+N Y++ ++ A+ F +++ LW L+D L+ L L +A ++ + PP G+S SL+ NI +L S D TA +TV+ + F LLSNLV + I+ + FL F ++ P KS +A N WL + +LS +GQ I+K GS + V ++ LLI+ N+C + K K++ N + + + CL+S+++ +Q+ S ALL+ K+
Sbjct: 1534 FSSLSMKNNKTWGKSHPVTH-YLLNSKSKQFHIKPTQKCL--------GHLLSTHLVDGCIQEIKHIHAQL------NLD-SLRPGKAAHKKKEE-----NHLKQLKVLMQILRNCLYQNEEGKAAAIDSQFPFVLHSLWPWLLMDDALMQGALQLLCVYTAYYPAACTSLCWTNTGVTTPQPPPKTGASNSLMHNILKLAS-DSTA---NNSTVQQMAFALLSNLVVIQDSKCIIQKSNFLQNFLTLSLPKAGTKSLCILA--NNWLKFLWNLSMGSDGQQMILKLKGSLDLLVELTTYKQKMISPVALLILHNVCFNTANKAKILSNDKA-VAVLSTCLESDNLLDQQIGCSAFWALLHGYQKV 1871 HSP 4 Score: 56.225 bits (134), Expect = 8.089e-7 Identity = 40/131 (30.53%), Postives = 69/131 (52.67%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNLLKVNSNDTILSENEGFSCSLL 441
++ V KL H L E+R RALKNI K++ ++++ DL + K F +LLEWFN V VL L+ + + +I++G ++F +QL+ ++D I+ L + + S+NE S++
Sbjct: 3 ISTLVKKLGHQLVEIRERALKNILCKLDHNLVSLVDLAQEKTFFLHLLEWFNFPAVPMKADVLHLLNKLVKHSEAAQWLIQLGAVDFFSQLRSNVEPDLQAVVDSILDGLFLLPTERPEPSQNENLLISVI 133
BLAST of Rotatin vs. Ensembl Xenopus
Match: RTTN (rotatin [Source:NCBI gene;Acc:100379860]) HSP 1 Score: 91.2781 bits (225), Expect = 2.177e-17 Identity = 203/1036 (19.59%), Postives = 390/1036 (37.64%), Query Frame = 2
Query: 1283 LSNKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFL-NNNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFIKCDE---ILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVNDNKHERWFPRLLNGQSKSLDI-DNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGG----LHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLN----VDIYDIFNETSL----WISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNS-LFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQG-----NTLKVISNVINLCG----------------------DLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLR------------------KSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLD 4201
L + R ++ + + SI E A L E L D + +I+++E + + K+S+ S E F L + E K + + AE +L L + ++F S +IF + + + +E +L LM + + +IV++SL I KP S+ I+FLL + + + FGL EI A + L +G+ +M W ++ + LQ D +G IL L +++++ I L ++K VR A +++ L + C+ Q + L ++V E+ L+ KS + D +K+ +I+LS +D +++KSAAEQ+ + Q MH+ G LHY+ + ++ D ++ +++L +IV R L + L +++L + + + +++L +++F+ + + D + ++ ++ R LP V Y A + S W + LR+ W + H G++ +L K + ++ +EI ++ L L+ ++ L + L D L I A++H +V+S++ + N LN ++ +SL W + +FLL P+C D LL ++ L I++ N +QW+++++ + S LF +E+ + K + ++ L FF LM +CL +T + + +HL++R + F G TL+ +S+V + G ++I F+ +++++MG GV + L+ HL E+ + TDW+ WL+ L R ++R S I S + ++ L C I LW +++L+
Sbjct: 427 LMSHRMAFISISLFTMRLLQSILPVEKANDVLPESLMNALFLISLDLPFRMAYPSVHESVISFMEQLNSEHCGIYKQSSETAYSIECATTFMLYSRKEGKKDFIELVELAEKALSSLPFHKHFPLVQKFIQICS-----DIFKSAQSSPVLLEESQKVLLCLMSHWMPLVKAEAYKTNLEIVKDSLGIHNVTKPVSSVCQGIHFLLHSKVLYEISTFGLQESNSEICTAAKTILSYLLQGRLVMENHTWNTLIESICPVIPVLQGFADTEETLGTSILTLCETSANDDGILPKTA---------RIRAALRQLFSKKQAVRSVAIKWIAVHLMNEEDSTCK-RPQILGSTLSNAVCLFILEQPLDLKLDNNEKSFFMEDTVQKVCEIFLSDTVDFALQKSAAEQMSVIAQDVKMHSVLKKPGVVDKILHYLYKYVQQ---DGKAFECMILPCLMLLRKIVYADPGFRLTLSQKPCFLLTLFRVSLMMQQEVTSLTEAAALLCMLLFDEISRMD--TCIFYVIYVLFLRFHLPVLVSAYHAVSPYTMASSLS-------ADWLTSKPVSVMLRMSWNISWHQGVDPFL---------KTVANEQ-----EEIENSLETLKLSPEEVLTLKMTHSVSGLQDCLSSIIQAVSHGEVRSAVARM-------NFYLLNDRLVLNCLTACYMSSLKCLPWHTALSRFLLVHPACEEDEKLLADVIRFLNKILQEQKGNSDPHDLQWILEMLLKQSPSPLFELMEQKEYPAKKDLDDTRAAVRQHLQKELMMFFNTLM------------------------------LCLTSVTDRKCLVLGGTFRTQLALHLLNRLKISDAPHFYGLPSLAQTLRGMSHVTSQPGWSAYSPSTEPFAICVKTLTSLLEIISSFYVEWGGNALSYMGKGVTKSTVLCLL-----HLSHEMTAQAKN----TDWIHLWTLPYDHGTEDQVLSRLGLEWLIPLWVDRDPEVRFTSLGIASALTCLQKGCLALSASCQNI-SGGLWGTVFSIFLN 1374 HSP 2 Score: 72.7886 bits (177), Expect = 8.205e-12 Identity = 41/135 (30.37%), Postives = 71/135 (52.59%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI-------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQNYGEYEEDNHWNSDDL 976
LK S P +PLT DK+VLS + +LRS++ +V Q DV+++DFP +FLQR +++ LL ++ ++ ++ +S+LC + + DPNF STK + + ++ Y D N+ +
Sbjct: 135 LKFSTFPWLPLTTTDKHVLSSNESSLRSNNPSLVWRSCQLLQDVVMQDFPPEIFLQRPKIVQSLLSLFGLASEKDGQQHLASQSISCLSQLCTLLRNRFSFHRDPNFLSTKQEI-VSHNSFASYCHDAQANNSQV 268 HSP 3 Score: 68.9366 bits (167), Expect = 1.067e-10 Identity = 92/366 (25.14%), Postives = 174/366 (47.54%), Query Frame = 2
Query: 5273 FTELLLKKRESLSRINAKSHSYTDTSINERINVHNTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTVK-LIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKI 6364
F+ L +K ++ + + +H Y S +++ ++ T K L +L+ ++ EIK++H++L N+D +R K + + + +K++ ++++L+N Y++ ++ A+ F +++ LW L+D L+ L L +A ++ + PP G+S SL+ NI +L S D TA +TV+ + F LLSNLV + I+ + FL F ++ P KS +A N WL + +LS +GQ I+K GS + V ++ LLI+ N+C + K K++ N + + + CL+S+++ +Q+ S ALL+ K+
Sbjct: 1521 FSSLSMKNNKTWGKSHPVTH-YLLNSKSKQFHIKPTQKCL--------GHLLSTHLVDGCIQEIKHIHAQL------NLD-SLRPGKAAHKKKEE-----NHLKQLKVLMQILRNCLYQNEEGKAAAIDSQFPFVLHSLWPWLLMDDALMQGALQLLCVYTAYYPAACTSLCWTNTGVTTPQPPPKTGASNSLMHNILKLAS-DSTA---NNSTVQQMAFALLSNLVVIQDSKCIIQKSNFLQNFLTLSLPKAGTKSLCILA--NNWLKFLWNLSMGSDGQQMILKLKGSLDLLVELTTYKQKMISPVALLILHNVCFNTANKAKILSNDKA-VAVLSTCLESDNLLDQQIGCSAFWALLHGYQKV 1858
BLAST of Rotatin vs. Ensembl Xenopus
Match: RTTN (rotatin [Source:NCBI gene;Acc:100379860]) HSP 1 Score: 90.8929 bits (224), Expect = 2.477e-17 Identity = 195/985 (19.80%), Postives = 372/985 (37.77%), Query Frame = 2
Query: 1460 LITYIELFDQNFAKLNKESNRLMQSSESLIKFFL-NNNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFIKCDE---ILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVNDNKHERWFPRLLNGQSKSLDI-DNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGG----LHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILE--------IIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLN----VDIYDIFNETSL----WISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNS-LFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQG-----NTLKVISNVINLCG----------------------DLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLR------------------KSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLD 4201
+I+++E + + K+S+ S E F L + E K + + AE +L L + ++F S +IF + + + +E +L LM + + +IV++SL I KP S+ I+FLL + + + FGL EI A + L +G+ +M W ++ + LQ D +G IL L +++++ I L ++K VR A +++ L + C+ Q + L ++V E+ L+ KS + D +K+ +I+LS +D +++KSAAEQ+ + Q MH+ G LHY+ + ++ D ++ +++L +IV R L + L +++L + + + +++L +++F+ + + D S I + R LP V Y A + S W + LR+ W + H G++ +L K + ++ +EI ++ L L+ ++ L + L D L I A++H +V+S++ + N LN ++ +SL W + +FLL P+C D LL ++ L I++ N +QW+++++ + S LF +E+ + K + ++ L FF LM +CL +T + + +HL++R + F G TL+ +S+V + G ++I F+ +++++MG GV + L+ HL E+ + TDW+ WL+ L R ++R S I S + ++ L C I LW +++L+
Sbjct: 533 VISFMEQLNSEHCGIYKQSSETAYSIECATTFMLYSRKEGKKDFIELVELAEKALSSLPFHKHFPLVQKFIQICS-----DIFKSAQSSPVLLEESQKVLLCLMSHWMPLVKAEAYKTNLEIVKDSLGIHNVTKPVSSVCQGIHFLLHSKVLYEISTFGLQESNSEICTAAKTILSYLLQGRLVMENHTWNTLIESICPVIPVLQGFADTEETLGTSILTLCETSANDDGILPKTA---------RIRAALRQLFSKKQAVRSVAIKWIAVHLMNEEDSTCK-RPQILGSTLSNAVCLFILEQPLDLKLDNNEKSFFMEDTVQKVCEIFLSDTVDFALQKSAAEQMSVIAQDVKMHSVLKKPGVVDKILHYLYKYVQQ---DGKAFECMILPCLMLLRKIVYADPGFRLTLSQKPCFLLTLFRVSLMMQQEVTSLTEAAALLCMLLFDEISRMDTWSEAIPFCTQPPFGLPVTVVRRFHLPVLVSAYHAVSPYTMASSLS-------ADWLTSKPVSVMLRMSWNISWHQGVDPFL---------KTVANEQ-----EEIENSLETLKLSPEEVLTLKMTHSVSGLQDCLSSIIQAVSHGEVRSAVARM-------NFYLLNDRLVLNCLTACYMSSLKCLPWHTALSRFLLVHPACEEDEKLLADVIRFLNKILQEQKGNSDPHDLQWILEMLLKQSPSPLFELMEQKEYPAKKDLDDTRAAVRQHLQKELMMFFNTLM------------------------------LCLTSVTDRKCLVLGGTFRTQLALHLLNRLKISDAPHFYGLPSLAQTLRGMSHVTSQPGWSAYSPSTEPFAICVKTLTSLLEIISSFYVEWGGNALSYMGKGVTKSTVLCLL-----HLSHEMTAQAKN----TDWIHLWTLPYDHGTEDQVLSRLGLEWLIPLWVDRDPEVRFTSLGIASALTCLQKGCLALSASCQNI-SGGLWGTVFSIFLN 1431 HSP 2 Score: 73.559 bits (179), Expect = 5.091e-12 Identity = 88/331 (26.59%), Postives = 160/331 (48.34%), Query Frame = 2
Query: 5378 TLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTVK-LIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKI 6364
TL +LL S+ + Y + ++ EIK++H++L N+D +R K + + + +K++ ++++L+N Y++ ++ A+ F +++ LW L+D L+ L L +A ++ + PP G+S SL+ NI +L S D TA +TV+ + F LLSNLV + I+ + FL F ++ P KS +A N WL + +LS +GQ I+K GS + V ++ LLI+ N+C + K K++ N + + + CL+S+++ +Q+ S ALL+ K+
Sbjct: 1864 TLILLLAVSRSARKYSLDAHLVDGCIQEIKHIHAQL------NLDS-LRPGKAAHKKKEE-----NHLKQLKVLMQILRNCLYQNEEGKAAAIDSQFPFVLHSLWPWLLMDDALMQGALQLLCVYTAYYPAACTSLCWTNTGVTTPQPPPKTGASNSLMHNILKLAS-DSTA---NNSTVQQMAFALLSNLVVIQDSKCIIQKSNFLQNFLTLSLPKAGTKSLCILA--NNWLKFLWNLSMGSDGQQMILKLKGSLDLLVELTTYKQKMISPVALLILHNVCFNTANKAKILSNDKA-VAVLSTCLESDNLLDQQIGCSAFWALLHGYQKV 2175 HSP 3 Score: 72.7886 bits (177), Expect = 8.793e-12 Identity = 41/131 (31.30%), Postives = 69/131 (52.67%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI-------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQNYGEYEEDNHWN 964
LK S P +PLT DK+VLS + +LRS++ +V Q DV+++DFP +FLQR +++ LL ++ ++ ++ +S+LC + + DPNF STK + + ++ Y D N
Sbjct: 178 LKFSTFPWLPLTTTDKHVLSSNESSLRSNNPSLVWRSCQLLQDVVMQDFPPEIFLQRPKIVQSLLSLFGLASEKDGQQHLASQSISCLSQLCTLLRNRFSFHRDPNFLSTKQEI-VSHNSFASYCHDAQAN 307 HSP 4 Score: 54.299 bits (129), Expect = 3.439e-6 Identity = 36/110 (32.73%), Postives = 60/110 (54.55%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNL 378
++ V KL H L E+R RALKNI K++ ++++ DL + K F +LLEWFN V VL L+ + + +I++G ++F +QL+ ++D I+ L
Sbjct: 3 ISTLVKKLGHQLVEIRERALKNILCKLDHNLVSLVDLAQEKTFFLHLLEWFNFPAVPMKADVLHLLNKLVKHSEAAQWLIQLGAVDFFSQLRSNVEPDLQAVVDSILDGL 112
BLAST of Rotatin vs. Ensembl Xenopus
Match: RTTN (rotatin [Source:NCBI gene;Acc:100379860]) HSP 1 Score: 90.8929 bits (224), Expect = 3.090e-17 Identity = 195/985 (19.80%), Postives = 372/985 (37.77%), Query Frame = 2
Query: 1460 LITYIELFDQNFAKLNKESNRLMQSSESLIKFFL-NNNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFIKCDE---ILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVNDNKHERWFPRLLNGQSKSLDI-DNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGG----LHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILE--------IIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLN----VDIYDIFNETSL----WISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNS-LFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQG-----NTLKVISNVINLCG----------------------DLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLR------------------KSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLD 4201
+I+++E + + K+S+ S E F L + E K + + AE +L L + ++F S +IF + + + +E +L LM + + +IV++SL I KP S+ I+FLL + + + FGL EI A + L +G+ +M W ++ + LQ D +G IL L +++++ I L ++K VR A +++ L + C+ Q + L ++V E+ L+ KS + D +K+ +I+LS +D +++KSAAEQ+ + Q MH+ G LHY+ + ++ D ++ +++L +IV R L + L +++L + + + +++L +++F+ + + D S I + R LP V Y A + S W + LR+ W + H G++ +L K + ++ +EI ++ L L+ ++ L + L D L I A++H +V+S++ + N LN ++ +SL W + +FLL P+C D LL ++ L I++ N +QW+++++ + S LF +E+ + K + ++ L FF LM +CL +T + + +HL++R + F G TL+ +S+V + G ++I F+ +++++MG GV + L+ HL E+ + TDW+ WL+ L R ++R S I S + ++ L C I LW +++L+
Sbjct: 533 VISFMEQLNSEHCGIYKQSSETAYSIECATTFMLYSRKEGKKDFIELVELAEKALSSLPFHKHFPLVQKFIQICS-----DIFKSAQSSPVLLEESQKVLLCLMSHWMPLVKAEAYKTNLEIVKDSLGIHNVTKPVSSVCQGIHFLLHSKVLYEISTFGLQESNSEICTAAKTILSYLLQGRLVMENHTWNTLIESICPVIPVLQGFADTEETLGTSILTLCETSANDDGILPKTA---------RIRAALRQLFSKKQAVRSVAIKWIAVHLMNEEDSTCK-RPQILGSTLSNAVCLFILEQPLDLKLDNNEKSFFMEDTVQKVCEIFLSDTVDFALQKSAAEQMSVIAQDVKMHSVLKKPGVVDKILHYLYKYVQQ---DGKAFECMILPCLMLLRKIVYADPGFRLTLSQKPCFLLTLFRVSLMMQQEVTSLTEAAALLCMLLFDEISRMDTWSEAIPFCTQPPFGLPVTVVRRFHLPVLVSAYHAVSPYTMASSLS-------ADWLTSKPVSVMLRMSWNISWHQGVDPFL---------KTVANEQ-----EEIENSLETLKLSPEEVLTLKMTHSVSGLQDCLSSIIQAVSHGEVRSAVARM-------NFYLLNDRLVLNCLTACYMSSLKCLPWHTALSRFLLVHPACEEDEKLLADVIRFLNKILQEQKGNSDPHDLQWILEMLLKQSPSPLFELMEQKEYPAKKDLDDTRAAVRQHLQKELMMFFNTLM------------------------------LCLTSVTDRKCLVLGGTFRTQLALHLLNRLKISDAPHFYGLPSLAQTLRGMSHVTSQPGWSAYSPSTEPFAICVKTLTSLLEIISSFYVEWGGNALSYMGKGVTKSTVLCLL-----HLSHEMTAQAK----NTDWIHLWTLPYDHGTEDQVLSRLGLEWLIPLWVDRDPEVRFTSLGIASALTCLQKGCLALSASCQNI-SGGLWGTVFSIFLN 1431 HSP 2 Score: 75.8702 bits (185), Expect = 8.728e-13 Identity = 90/336 (26.79%), Postives = 162/336 (48.21%), Query Frame = 2
Query: 5378 TLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTVK-LIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLK 6379
TL +LL S+ + Y + ++ EIK++H++L N+D +R K + + + +K++ ++++L+N Y++ ++ A+ F +++ LW L+D L+ L L +A ++ + PP G+S SL+ NI +L S D TA +TV+ + F LLSNLV + I+ + FL F ++ P KS +A N WL + +LS +GQ I+K GS + V ++ LLI+ N+C + K K++ N + + + CL+S+++ +Q+ S ALL+ K + LK
Sbjct: 1864 TLILLLAVSRSARKYSLDAHLVDGCIQEIKHIHAQL------NLDS-LRPGKAAHKKKEE-----NHLKQLKVLMQILRNCLYQNEEGKAAAIDSQFPFVLHSLWPWLLMDDALMQGALQLLCVYTAYYPAACTSLCWTNTGVTTPQPPPKTGASNSLMHNILKLAS-DSTA---NNSTVQQMAFALLSNLVVIQDSKCIIQKSNFLQNFLTLSLPKAGTKSLCILA--NNWLKFLWNLSMGSDGQQMILKLKGSLDLLVELTTYKQKMISPVALLILHNVCFNTANKAKILSNDKA-VAVLSTCLESDNLLDQQIGCSAFWALLHGYQKAKATLK 2180 HSP 3 Score: 72.7886 bits (177), Expect = 8.588e-12 Identity = 41/131 (31.30%), Postives = 69/131 (52.67%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI-------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQNYGEYEEDNHWN 964
LK S P +PLT DK+VLS + +LRS++ +V Q DV+++DFP +FLQR +++ LL ++ ++ ++ +S+LC + + DPNF STK + + ++ Y D N
Sbjct: 178 LKFSTFPWLPLTTTDKHVLSSNESSLRSNNPSLVWRSCQLLQDVVMQDFPPEIFLQRPKIVQSLLSLFGLASEKDGQQHLASQSISCLSQLCTLLRNRFSFHRDPNFLSTKQEI-VSHNSFASYCHDAQAN 307 HSP 4 Score: 54.299 bits (129), Expect = 3.218e-6 Identity = 36/110 (32.73%), Postives = 60/110 (54.55%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNL 378
++ V KL H L E+R RALKNI K++ ++++ DL + K F +LLEWFN V VL L+ + + +I++G ++F +QL+ ++D I+ L
Sbjct: 3 ISTLVKKLGHQLVEIRERALKNILCKLDHNLVSLVDLAQEKTFFLHLLEWFNFPAVPMKADVLHLLNKLVKHSEAAQWLIQLGAVDFFSQLRSNVEPDLQAVVDSILDGL 112
BLAST of Rotatin vs. Ensembl Mouse
Match: Rttn (rotatin [Source:MGI Symbol;Acc:MGI:2179288]) HSP 1 Score: 100.523 bits (249), Expect = 2.664e-20 Identity = 217/1041 (20.85%), Postives = 406/1041 (39.00%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI-------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFF-------STKNTFGIINQNYGEYE-------------------------EDNHWNS------------DDLLGFHYEPNFG---LPE------------PLPVPVFCLQVINVCLYHILEIDTDSNWAELFHLICTAIELFRESFGSDLRQPYLLELWNLKDQINDVLTLWSRLLKQYSIIEDMNYRKLS-----------NKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFLN-NNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLI-----SSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRN-----FQNNIIKFLPLFYNKNYDVRKEACQYL------EQFLRFDRLWNCQLNIQNMTD-FLVSSVNDNKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKI-LDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRV-GKYSANKTSKTEWTFSNFS---KIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLN-VDIYDIFNETSLWISTFHKFLLNIPSCSADFILLEFIMDVLENIIK 3412
L+ SV P +PLT D++VLS + +LRS + ++ + + DVI++DFP+ +FLQR +++ LL + + + + +V + +LC + +N DPNFF S ++ ++ G Y + W++ + + H + LPE L +P FC+ + +L + + L+ + L ++ S++ L N+K+++ VL S+ E M YRK S + R ++ + + + E A ++L E L D + L E ++ Y+E D + K + + S E F + E K + + A+ +L Y KE S I +S + G + +F+ +K + C +IV+ L ++ KP S+ + I+FLL + + FG+ E++ A A+ L +G+ +M+ + W + + + LQ D +G IL L K+S++ + L L K VR A + L E+ R + + +T+ F+V D K L + + + ++ KK+ DI+ S ++D+ +RKSAAEQL + +Q MH + ++ L + D + L+ + +L +++ IR +L + L+ L++++L F D VA++ ++ L++F+ V + D S + + LP V +Y S +S +P + + + LRI W L + G +N L+ S KI + D L E + + K+T T+S L D L I A H V++++ + L+ + + + + ++ W + ++FL +P+C+ D LL I+ L +IK
Sbjct: 170 LRFSVFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQGLLCLVKLAFGGDGKHRLALQSVSCLQQLCTSLRNRLNFYRDPNFFSSRQDTVSQNSSLSYCHEARGPYHSPNPSPGSSSSRPSVVGRTGQRPRGDGQDWDAVSSSGSSSHTHVNSRISVHSPLDVAPVDLPELEAEDTLELQFQQLSLPQFCISTLEAA-APLLRTGSREMIIRVLELLAEDMLLIGQAISSEIWDDNSLFAINVKEKLLQVLG---------SLGETMCYRKTSFSMEQAEAVLVHHRMAFVSISLFTVRLLQLLLPVEKASEFLPESMSAALFFVSLDMPISLEYPEIHDTVVAYLEQLDSENYSIYKRTAEAVYSIECTCNFLSDVGKEGEKNLLELVELADQALRSFSYHQHSPLIKEIICICSKIWKSAQASPLLQGESQKVFLHMLSHPLPQVKVETYQCC-------LEIVKECLGVQNVTKPVSSLCSGIHFLLHPKVLYEISAFGIQESKNEVNAAAKAILLYLLQGRLMMTALTWNKFIESLAPVIPVLQGYADTEDPLGNCILLLS-------------KVSSEAGEGALPCTARLKSVLRLLLVKKPSVRSLALKLLAFHLTSEEGADKKRPFIDAGVLSRVTNLFIVKKPIDLK-------LDDRRELVIKLETVKKVCDIFTSDDVDLVLRKSAAEQLAVIMQDIKMHTVVKKLCLVEKIIGHLNEFVGQDGEVIECLIQPCLTLLRKVLYADPVIRVSLSQQASLLTLLLRVSLIFHEDCTVVAEVGALFCLLLFDEVSRMDTWSDTLSDKPSLSSVFSLPVSVFRRYHLPVHVTGHHAVSPYSIVLPLPTECLTLKPVSDM-LRIAWNLAWYHGSDNLLKQMTS--EAKIQEFLDTLQLSTEDILAL-KITYTDSGLK------------DCLQSIIQAGGHRDVRAAITRMSFYLLNDKLSLKDDIGPCGVTLKSLAWHTVLNRFLQVLPACTEDEKLLIDIIHFLNKLIK 1157 HSP 2 Score: 64.6994 bits (156), Expect = 1.975e-9 Identity = 84/346 (24.28%), Postives = 159/346 (45.95%), Query Frame = 2
Query: 5402 SQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTI---------NTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTV--KLIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKL 6403
S+ + + +R IE+ ++K+++ AQ N+D+ +R K + + IKE+ ++LL+N Y++ + A+ +++ LW L++ L+ L L +A+ N + I H+ S SLI I +L S + A ENT +L+F+ LSNL + ++ + FL F + P + S +AI LWL L++++SF E+GQ I++ +G + + + LLI N+C P K K++ N ++ + CL+S + +++ ++L AL Y+ K + LK+ ++ K+
Sbjct: 1874 SRRAQRHALRSDLIENCMEQMKHIN------AQLNLDL-LRPGKAVLKKKED-----GFIKELSITMQLLRNCLYQNEECKVAALEARLVPVLHSLWPWLLMEDSLMQIALQLLCVYTANFPAGCSSLCWSNYGQCIQTAHR----------GTPSSSLILCILKLAS-QMPA----ENTTIQQLVFMFLSNLALSHDCKGVIQKSNFLQHFLSLTLPKGGNQHLSTVAI--LWLKLLLNMSFGEDGQQMILRLDGCLDLLIEMSKYKHKSSPHMPLLIFHNICFSPANKPKILAN-EKVVTLLAACLESENQTAQRIGAASLWALTYNYQKAKATLKNPSIKRKV 2189 HSP 3 Score: 53.9138 bits (128), Expect = 3.470e-6 Identity = 33/92 (35.87%), Postives = 52/92 (56.52%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLK 324
L + KL H L E+R RALK+I K+E +I DL + + +LLEWFN V + VL L+ ++ + +I +G +EFL++L+
Sbjct: 3 LAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERLLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYSPAVQHLIDLGAVEFLSKLR 94
BLAST of Rotatin vs. UniProt/SwissProt
Match: sp|Q8R4Y8|RTTN_MOUSE (Rotatin OS=Mus musculus OX=10090 GN=Rttn PE=1 SV=2) HSP 1 Score: 100.523 bits (249), Expect = 1.864e-19 Identity = 217/1041 (20.85%), Postives = 406/1041 (39.00%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI-------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFF-------STKNTFGIINQNYGEYE-------------------------EDNHWNS------------DDLLGFHYEPNFG---LPE------------PLPVPVFCLQVINVCLYHILEIDTDSNWAELFHLICTAIELFRESFGSDLRQPYLLELWNLKDQINDVLTLWSRLLKQYSIIEDMNYRKLS-----------NKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFLN-NNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLI-----SSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRN-----FQNNIIKFLPLFYNKNYDVRKEACQYL------EQFLRFDRLWNCQLNIQNMTD-FLVSSVNDNKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKI-LDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRV-GKYSANKTSKTEWTFSNFS---KIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLN-VDIYDIFNETSLWISTFHKFLLNIPSCSADFILLEFIMDVLENIIK 3412
L+ SV P +PLT D++VLS + +LRS + ++ + + DVI++DFP+ +FLQR +++ LL + + + + +V + +LC + +N DPNFF S ++ ++ G Y + W++ + + H + LPE L +P FC+ + +L + + L+ + L ++ S++ L N+K+++ VL S+ E M YRK S + R ++ + + + E A ++L E L D + L E ++ Y+E D + K + + S E F + E K + + A+ +L Y KE S I +S + G + +F+ +K + C +IV+ L ++ KP S+ + I+FLL + + FG+ E++ A A+ L +G+ +M+ + W + + + LQ D +G IL L K+S++ + L L K VR A + L E+ R + + +T+ F+V D K L + + + ++ KK+ DI+ S ++D+ +RKSAAEQL + +Q MH + ++ L + D + L+ + +L +++ IR +L + L+ L++++L F D VA++ ++ L++F+ V + D S + + LP V +Y S +S +P + + + LRI W L + G +N L+ S KI + D L E + + K+T T+S L D L I A H V++++ + L+ + + + + ++ W + ++FL +P+C+ D LL I+ L +IK
Sbjct: 170 LRFSVFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQGLLCLVKLAFGGDGKHRLALQSVSCLQQLCTSLRNRLNFYRDPNFFSSRQDTVSQNSSLSYCHEARGPYHSPNPSPGSSSSRPSVVGRTGQRPRGDGQDWDAVSSSGSSSHTHVNSRISVHSPLDVAPVDLPELEAEDTLELQFQQLSLPQFCISTLEAA-APLLRTGSREMIIRVLELLAEDMLLIGQAISSEIWDDNSLFAINVKEKLLQVLG---------SLGETMCYRKTSFSMEQAEAVLVHHRMAFVSISLFTVRLLQLLLPVEKASEFLPESMSAALFFVSLDMPISLEYPEIHDTVVAYLEQLDSENYSIYKRTAEAVYSIECTCNFLSDVGKEGEKNLLELVELADQALRSFSYHQHSPLIKEIICICSKIWKSAQASPLLQGESQKVFLHMLSHPLPQVKVETYQCC-------LEIVKECLGVQNVTKPVSSLCSGIHFLLHPKVLYEISAFGIQESKNEVNAAAKAILLYLLQGRLMMTALTWNKFIESLAPVIPVLQGYADTEDPLGNCILLLS-------------KVSSEAGEGALPCTARLKSVLRLLLVKKPSVRSLALKLLAFHLTSEEGADKKRPFIDAGVLSRVTNLFIVKKPIDLK-------LDDRRELVIKLETVKKVCDIFTSDDVDLVLRKSAAEQLAVIMQDIKMHTVVKKLCLVEKIIGHLNEFVGQDGEVIECLIQPCLTLLRKVLYADPVIRVSLSQQASLLTLLLRVSLIFHEDCTVVAEVGALFCLLLFDEVSRMDTWSDTLSDKPSLSSVFSLPVSVFRRYHLPVHVTGHHAVSPYSIVLPLPTECLTLKPVSDM-LRIAWNLAWYHGSDNLLKQMTS--EAKIQEFLDTLQLSTEDILAL-KITYTDSGLK------------DCLQSIIQAGGHRDVRAAITRMSFYLLNDKLSLKDDIGPCGVTLKSLAWHTVLNRFLQVLPACTEDEKLLIDIIHFLNKLIK 1157 HSP 2 Score: 64.6994 bits (156), Expect = 1.381e-8 Identity = 84/346 (24.28%), Postives = 159/346 (45.95%), Query Frame = 2
Query: 5402 SQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTI---------NTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTV--KLIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKL 6403
S+ + + +R IE+ ++K+++ AQ N+D+ +R K + + IKE+ ++LL+N Y++ + A+ +++ LW L++ L+ L L +A+ N + I H+ S SLI I +L S + A ENT +L+F+ LSNL + ++ + FL F + P + S +AI LWL L++++SF E+GQ I++ +G + + + LLI N+C P K K++ N ++ + CL+S + +++ ++L AL Y+ K + LK+ ++ K+
Sbjct: 1874 SRRAQRHALRSDLIENCMEQMKHIN------AQLNLDL-LRPGKAVLKKKED-----GFIKELSITMQLLRNCLYQNEECKVAALEARLVPVLHSLWPWLLMEDSLMQIALQLLCVYTANFPAGCSSLCWSNYGQCIQTAHR----------GTPSSSLILCILKLAS-QMPA----ENTTIQQLVFMFLSNLALSHDCKGVIQKSNFLQHFLSLTLPKGGNQHLSTVAI--LWLKLLLNMSFGEDGQQMILRLDGCLDLLIEMSKYKHKSSPHMPLLIFHNICFSPANKPKILAN-EKVVTLLAACLESENQTAQRIGAASLWALTYNYQKAKATLKNPSIKRKV 2189
BLAST of Rotatin vs. UniProt/SwissProt
Match: sp|Q86VV8|RTTN_HUMAN (Rotatin OS=Homo sapiens OX=9606 GN=RTTN PE=1 SV=3) HSP 1 Score: 93.2041 bits (230), Expect = 3.020e-17 Identity = 218/1036 (21.04%), Postives = 412/1036 (39.77%), Query Frame = 2
Query: 1301 IYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFLN-NNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLI-----SSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVND---NKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKI-LDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRV-GKYSANKTSKTEWTFSNFSKIPNQKWN--YQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETSL-WISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMT--TPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYP-FDNLSKLLIHIHLMSRSLELKSNF--FQG-NTLKVISNVINLCG-------DLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWL--------------RKSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSM 4285
I + LFA+ + ++ E A ++L E T L D + L ++ Y+E + + K + + S E F + E K + + A+ +L Y KE S I +S + G + + + +K+ + C +I + L + KP S+ N I+FLL + + +FG+ E++ A A+ L +G+ +M+ + W + + + LQ D +G IL L ++S + L L K VR A + L L + + + + + ++S V D K RL + + + ++ +K+++I+ S ++D+ +RKSAAEQL + +Q MH + ++E L + D + LV + +L +++ +R +L S L+ L +++L F D V ++ ++ L++F+ V + D S + LP V +Y S +S + + + LRI W L + G +N L K++ +++D L L+ D+ L + L D LH I A H +V++++ + L+ + SL W + ++FL +P+C+ D LL I+ L +IK N + + W+++++ + N L + + +TD R T + +++ L F L+ LN ++++ ++ S+++ + + + L Q + + P F L L R+L +N F G ++ ++ +++C ++I F+ R ++++FMG GV + L+ HL E+ + + W + WL+ L R ++R S + S + E L C I LW + + LD SE +VR EA ILQNLL +P P +
Sbjct: 474 ISISLFAV--RLLQTLLPVEKASEFLSEPMSTALFLLSLDMPISLEYPNIHEAVVAYLEQLNSENYSIYKRTAEAVYSIECTCNFLSDIGKEGEKNLLELVELADQALRSFSYHQHFPLIKEIISICSKIWKSAQASPLLQGESQKVLLHMLSHPLPRVKAETYHCC-------LEITKECLGVHNVTKPVSSLCNGIHFLLHPKVLYEISVFGIQEPESEVNTAAKAILLYLLQGRLMMTALTWNKFIESLCPVIPILQGYADTEDPLGNCILLLSKASS---------DTEEMLPCTTRLKSMLRLLLVKKPSVRSLALKLLAFHLTSEEGADTKRPLIDAR--VLSRVTDLFIGKKPIEL-RLDDRRELVIKLETVEKVYEIFTSDDVDLVLRKSAAEQLAVIMQDIKMHAVVKKLCLIDKIIEYLNECVSQDGKVVECLVQPCLTLLRKVLCGDPVMRVSLSQQSSLLTVLFRVSLIFHEDCSVVTEVGALFCLLLFDEVSRMDMWSVNPSNKPSLPSVFSLPVSVFRRYHLPVHVIGHHAVSPYSIVLPLSADCLALKPVSDMLRIAWNLSWYHGSDNLL--------KQMNSETKTQEILD-------ALKLSTEDILTLKITHMASGLQDCLHSIVQAATHREVRAAVTRMSFYLLNDRLSLKGCPGPCGVTLKSLAWHTALNRFLQVLPACTEDEKLLIDIIHFLNKLIKEQRKNSSLELLNWILELLLRHSANPLLDLLVLTESQAREETDDIR-TAVRQQLQKELIALFDTLL----LNFMEVTDRKC---SELLYVFQTQLALKLLQCLKVTDAPHFYGLPSL-------ERTLRGMANLTAFPGWSSHSPLTKPLDICVKYLSGLLEVITSFYVERGGNAMSFMGKGVTKSTILCLL-----HLSHEMMAQAGSLEWMSLWFLPLGSHSEEHIPTQQGLAWLIPLWVDRDPEVRFTSLGLGSALTTLETGCVALANSCQNI-SGGLWGTVVNILLDQSECSMVRREAAFILQNLL-VIPMPTEI 1451 HSP 2 Score: 59.6918 bits (143), Expect = 4.332e-7 Identity = 93/386 (24.09%), Postives = 177/386 (45.85%), Query Frame = 2
Query: 5375 NTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTV-KLIFLLLSNLVWCPEGINILLRIRFLDKFEQIN-PNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNG-----SGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANL---VTKLMSL-REIYENDSKNGL-------LLDIISLLNA 6478
N L LL S+ + + ++ I++ ++K+++ AQ N+D +R K ++ + +IKE+ ++LL+N Y++ + A+ +++ LW L+D L+ L L +A+ N ++ S SL+ I +L S L TV +++F+LLSNL + ++ + FL F + P K S + I L L++++S E+GQ I++ +G + + K + + +L LLI N+C P K K++ N +I + CL+S + +++ + L AL+Y+ K + LKS ++ V + SL ++ + N N L L +++ LLN+
Sbjct: 1864 NALMSLLAVSRRAQKHALKANLIDNCMEQMKHIN------AQLNLD-SLRPGKAALKKKED-----GVIKELSIAMQLLRNCLYQNEECKEAALEAHLVPVLHSLWPWILMDDSLMQISLQLLCVYTANFPNGCSSLCWSSCGQHPVQATHRGAVSNSLMLCILKLAS----QMPLENTTVQQMVFMLLSNLALSHDCKGVIQKSNFLQNFLSLALPKGGNKHLSNLTILW--LKLLLNISSGEDGQQMILRLDGCLDLLTEMSKYKHKSSPLL-----PLLIFHNVCFSPANKPKILAN-EKVITVLAACLESENQNAQRIGAAALWALIYNYQKAKTALKSPSVKRRVDEAYSLAKKTFPNSEANPLNAYYLKCLENLVQLLNS 2225 HSP 3 Score: 56.9954 bits (136), Expect = 2.730e-6 Identity = 42/123 (34.15%), Postives = 66/123 (53.66%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNT-SNFQI-LDQIIKNLLKVNSNDTILS 411
L + KL H L E+R RALK+I K+E +I DL + ++ +LLEWFN V + VL L+ ++ + ++ +G +EFL++L+ SN N Q +D I+ L + S LS
Sbjct: 3 LAGLIRKLGHQLAEIRERALKSILCKIEHNLICYADLIQERQLFLHLLEWFNFPSVPMKEEVLNLLSRLVKYPPAVQHLVDVGAVEFLSKLR--SNVEPNLQAEIDGILDGLFLLPSEVPALS 123 HSP 4 Score: 55.8398 bits (133), Expect = 6.485e-6 Identity = 38/119 (31.93%), Postives = 67/119 (56.30%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQN 931
LK S P +PLT D++VLS + +LRS + ++ + + DVI++DFP+ +FLQR +++ LL + + + + +V + +LC + +N DP FFS K+ ++QN
Sbjct: 170 LKFSTFPWLPLTTTDRHVLSSNESSLRSSNHTLIWNTCELLKDVIMQDFPAEIFLQRPKIVQSLLSLLKLAFGDGKHRLALQSVSCLQQLCMYLRNRLNFHRDPGFFSNKH--DTVSQN 286
BLAST of Rotatin vs. TrEMBL
Match: A0A210R0J0 (Rotatin OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT13424 PE=4 SV=1) HSP 1 Score: 310.842 bits (795), Expect = 4.889e-81 Identity = 422/1829 (23.07%), Postives = 772/1829 (42.21%), Query Frame = 2
Query: 1265 DMNYRKLSNKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFF-LNNNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQL------NIQNMTDFLV----SSVNDNKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKILDKSHLSIL--VP---STILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQD-----NLSAEIKILEIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETSLWISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKD-FIQWLVQIMTTPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYP----FDNLSKL------LIHIHLMS----RSLELKSNFFQGNTLKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLRKSP--------------------WLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSMEIPIDVNQN----DENEDISSLFIDRNSSGKYQEINGFYQKLLYL--DFFRKAGLSLTNFYTLPFINCSYFKHKPLYDPEYQINFDSDFQKSVNINQILSQMR------------------------------TACEI---NISTPLMISALVSFLCNLYYYLPDQVSSQFNELRINTLIM---NMFDPHTLKVYMESFQTEHYSTTVDLVLA-----FTNCVKLIRLQINMNSSLKDSFLIDEKFIETIIQLLIVTKPTSDFDESHDNRLMKLDLYKYISQLRVEILYLLISLVIPTPIKNHVQLMLAHV--ASHVTGIFNCIKEMIEIESRKSGNVALCFVSVFLCLSSSESKFNSLIRSLESQRFSNS--------ERVIIEDFTELLLKKRESLSRINAKSHS---YTDTSINERINVHNTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTVKLIFLLLSNLVWCPEGINILLRIRFLDKFEQINPNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKL 6403
D N L R Y+ + + ++ + A L E+ + +D+ L E + L+T ++L D++ ++ ++ ++ QS KF L E K + + + AE SL L+Y + EF S + + +D+ C ++L + + + + + + IV+ S+++ EA+ P S + FL ++ +++FGL ++ ++ +TA L C L + +M+ +W++ M+ + + LQ DL TR+G+ +L + S K + + + L ++ + R EA + + FL ++ +L ++ N+T+ V S++D+ F ID +K+ DI+ S ++D V+KSAA+QL I LQ N+H+ F + GL VLE LK IL S + +P + + IL +V H +R L D + LI+ A D++ + + L L++F+ V K D N I E++ R LPFR +KTS + P + L++ W + HGG++ L + K+ D+ KL L+ SD ++ + + + + + I NA +H V ++L + L+ A + + D+FN W +T +FL PS +AD LL+ ++ + I L +TN + D +QWL +++ P+ +L L + D K ++ L +F + +SK +KI ++ D+ +QLTQ LN F NL+ L L+HI S EL S + S V++ +++ FH GR +S+++MG GV L HL E + T +W R+ W++ L YR ++RA I + E R ++ HC I P +W A ++ LD +E +VR +A +L N+ + S+E+ +V Q D ++S + + + + + FYQ++L L +F+ + + + +P + S + D Q + NQ S+ R T ++ +I+TP ++S++ FL NL P F LR ++ I NM D + L YM + E S ++LV + + L+R + ++ ++ L +E FI + LL + F+ S D L+ + +L L + + H M AH S + + +++ + + S N L F+S+ S E + L + + +D E+ + IN HS D ++ I+ L+ LL S K+ ++ G +ES +K H+KL N++ I+ K + ++ ++ ++I +LL+NF ++ + A+ G I++RLW LD ++ L+ L A ++ GQ Q S+ SL+ + RLT + +++ T++ +F LL+ L EG NI+ + FL +F +NP + +KS ++ WL L+++LSF+ EGQ ++K+ + + V++ ++ +LI+RNMC H K KL+ + L+ ++ CL S++ I+ ++ S L AL+++N K +V +K+AN++ KL
Sbjct: 505 DNNKSGLIPHRLAYIGVCGFLNQLLKNLVPFQKARSLLPENLVKAVTVVVYDESLSHSYPEIQITLLTVLQLLDKDRYQVYVDTAKVAQSMHKSCKFLMLCQQEAYKGSVELATLAEASLLSLQYHLHLPLVSEFVKLTSSVCARH--SGDQDLQGHCRQVLLKYLSYPLVQVRQQAYNTVLQIVKGSVSVSEASDPSSEACLLGRFLFDTDVLYQIMVFGLDDKDIKVAKTAADLLCHLLDSPLMMTSQMWQEFMDSLLRPLPVLQSYSDLETRLGKCVLAMSDPQMS--------KGPSSLSSLEKLRATLRHMFSSDVRTRAEALKRVAWFLSNEKGSTTKLPVFSDLDVTNLTNIFVMETPRSIDDDLGRSVF-----------QIDGLRKVNDIFKSMSVDPGVKKSAADQLAIILQDHNLHSAFKRDRGLESVLEHLKLAILRDQGPSKIDSLPYLNACVTILRYLVHHDYTLRHRLASDEEIYTCLIRAAFIHQRDERTCYEAAHTLTLLLFDEVAKFDMGGGQNPVVTFSIPEVLKKRYRLPFR--PVCHHKTSPHQG-----PSAPEPDPLENGPPDEMLKVVWNVAWHGGMDKLLSHLKLLKAKQ-----------DKFTEFSVKLHLSCSDRVIIQATHLRQGMQEAVFDISNATSHKGVSAALNRLLTYLVT---AQGHPGVEDVFNLD--WFTTIGRFLKVTPSLTADESLLQEVLKFI--CIALKLTNGVPDNTLQWLGEMLYQPSGTLIGLLHRASVKGEARDVPENVNIKRTLDKELLSFIAM-----------YNSKLPYLLCRRLKIQQLRGDLS-HQLTQRLNVTDAPHFYNLASLEGTLQCLMHITARPGWSHESTELDSG-------ALCSQVLHCLLEVVSAFHIGRGGTSMSYMGKGVTKS-----ATLCLRHLAHE----MATYSDDKNWARQWSHMQQKSDASRSVLTGDSGLIWMLTLWAYRDPEVRAAGLGIAVALTSTEPGRIVMTSHCKHI-PGGIWGAAFSVLLDQAECSMVRQQAALLLVNMTSQTMPCGSVELEQNVWQGPIVTDTEFEVSLVGLTALLALLHH--SQFYQEMLVLLCNFYAQPTIQPVSVLEVPVGHLSSTGSDSTLSTLAEFGTDG-LQSTSGSNQPRSKGRQHIHQTSISSGSKTRNSTTDPTGNSSSSTLTGEQLEYQSIATPSLVSSVCQFLRNLVILAPQDT---FTCLRKDSYIQVLTNMVDTNLLDAYMTEME-EGCSDQLELVFCDLLHMYAAILDLLRACVVYDTPIRQEVLANEGFIRSTAALLTIR-----FEGSADVDGCCQALWSSVLSFLTALLQLQGASAL------HSFTMAAHTLWTSMAETLQSVLQKRLHLNKHLSDN-CLQFLSILF---SEEGRLVGRQPQLAEDTITVTGLLNTPLKSETTDKDEGEVTTGGQLCKVLINVYEHSCLKSPDYKQSDHIHAATCLRTLLAISSSAKDTALQSGLVESVIDHVKQTHAKL------NME-SIQPGKPNTRKKD-----DPLLSDLILTFDLLRNFIFQSLEAKMACYYSGLHNIIHRLWAWCQLDLAVMSSALSLLTTFIAHCPTATSSLAYTTTSASSGGQKSQQLSNNSLVHCLIRLTGRE----GIKDTTLRTLFSLLATLTLSSEGRNIVFKSNFLREFLSLNPRKSKKSKRMQNLELYWLELLVNLSFSVEGQQMLLKTGDCVDLLLDFVESGQGKVQECAVLILRNMCCHTSNKPKLLAS-DKLLPHLLECLTSDNTQIQCVAASALWALVFNNQKAKVMMKNANVIPKL 2219
BLAST of Rotatin vs. TrEMBL
Match: K1QRC4 (Rotatin OS=Crassostrea gigas OX=29159 GN=CGI_10027200 PE=4 SV=1) HSP 1 Score: 206.838 bits (525), Expect = 2.298e-49 Identity = 241/1068 (22.57%), Postives = 452/1068 (42.32%), Query Frame = 2
Query: 1415 FDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFF-LNNNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFI--KCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWN------CQLNIQNMTDFLVSSVNDNKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKIL----DKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKIL-----EIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETSLWISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYP----FDNLSKL------LIHIHLM----SRSLELKSNFFQGNTLKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWL-----------------RKSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFP-NSMEIPIDVNQNDENEDISSLFIDRNSSGKYQEINGFYQKLLYLDFFRKAGLSLTNFYTLPFIN 4468
FD+ L + + ++ YI+ + L ++ ++ QS + +F L E K + + I AE S+ L Y + EF S I + +D + KC E+L + + + + ++ I +V+ SL + EAA P S + FL ++I +I+FGL + ++ +TA+ + C + +GQ +M+ +W++V+N + + L+ D+ T++G S ++ + + + + + L + ++ + R E+ + L FL ++ N L++ N+T + + + E G+S ID +K+++I+ SS++D SV+KSA +QL I LQ N+H F + GG+ VL+ LK ++ +S +P+ IL +V ++R L D + L+++A D++ +++ IL L++F+ V K D + ++ ++ R LPFR + N E P+ + LRI W + HGG+ L++ + N D++ +L L+ + ++ S + L+ + NA +H V + + L ++ ++ + ++ +F + W+S+ +FL PS D LL+ ++ + ++LS + +Q L +++ P+ L + + G+T R I+ L L + N + ++ +K ++ D+C +QL Q LN F NL+ L L+H+ S S EL L + V++ FH GR +S+++MG GV L HL +E+ E W+ + WL+ L YR ++R I + E R++L HC I P +W VA ++ LD SE +VR +A +L N+ + P P S+E+ +EN + D S + L + F+++ + TNFY P I
Sbjct: 545 FDEALAYSYPDIQITVLAYIQNLSPDMYTLFVDTAKISQSVQKSCQFLMLCQEEAYKGSSELIDMAEASIQSLPYHLHLPLVSEFVKLSSSICAR----TSQDKALQNKCVEVLLKFLSHPLPEVKEQTYSTIKQVVKGSLNVNEAADPSSRACYLSRFLFSSDIMYEIIVFGLNDSNPKVSQTASDVICHILDGQLLMTEDLWKEVLNALMRSLPVLETYVDMGTKLG--------STLLSLTDPTEVQAEGGLVYLEKLRGCLRMMFSTDVRARAESLKRLAWFLSNEQNSNEKLPIFASLDVTNLTSIFIVDMQRSLDEDL------GRS-VFQIDGMRKVYEIFSSSSVDPSVKKSAVDQLAIILQDHNLHAPFKAEGGVETVLKHLKLGLVRNDDAQSEYIHYLPACAAILRSLVHFDYKLRHRLARDQEVYLVLLRVAFLHQRDERTCYEVAHILILLLFDEVAKFDLGGGTVPVVHFSIPSVLRKRCRLPFRPACHHDNSPHTVELP-------PDPDPVQTDRPLEMLRISWNVAWHGGMEKLLKH---------LAQSKNSSQKDKMTEFSPRLQLSFIERKIVQCSHLAEGIKQTLYSVSNATSHNAVS-AALGQLLSYLVNSYGYAGSEV--MFGQE--WLSSIGRFLKVHPSMPDDESLLQEVLRFISMALRLS-GEVTEGTLQALGEVLYQPSGPLIGLSHR-TSVRGET---RDVPENVNIKRTLDKELLSFISTYNCKLPYVLCRR-------LKFQQLRGDLC-HQLLQRLNVTDAPHFYNLASLEGTLQCLMHVTARPGWSSESTELGGEALCSQLLACLLEVVS-------AFHIGRGGTSMSYMGKGVTKAATMCL-----RHLAYEMSTTAENKKWAEQWIYVQQRADGQNSAEVEEHQGLDWLLTLWSYRDPEVRVAGLGIGVALTLTESGRTVLTSHCVHI-PGGVWGVAFSILLDQSECSMVRQQAALLLVNMTSQ-PMPCGSVEL-------EENVWQGPVVADTESKVSLVGLTALMALLHHSHFYQEMLVVFTNFYAQPTIQ 1538 HSP 2 Score: 95.5153 bits (236), Expect = 1.696e-15 Identity = 93/368 (25.27%), Postives = 177/368 (48.10%), Query Frame = 2
Query: 5339 TDTSINERINVHNTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXI----SLIENICRLTSADLTAYYLRENTVKLIFLLLSNLVWCPEGINILLRIRFLDKFEQINPNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKLMSLREIYEN 6430
TD +R++ L+ LL SQ K +++GF+E+T IK LHSK++ A ++ + ++V ++I L++L+N + + +A+ G +++++W ++ L+ ++ L +A + ++ + Q S SL+ + +L D ++E +K ++ LL+ L E NI+ + F +F ++NP + +KS ++ WL L+++LSF+ EGQ I+K+ S + + V ++LIIRNMC H K KL+ N L + L+ + ++ ++ S L L+++N K +V +K+ANL+ K L+EI N
Sbjct: 1868 TDHKNTDRLHSAAALRNLLAVSQKAKGTALQMGFVETTIENIKQLHSKINMEALQLGKTAAKKKEDALV------------PDLIITLDVLRNLLFMNEDAKMACYHSGLPTVIHKIWPWCQMEPQLMLSTVSLLSTYAAKCLTATSSLAYTSPSTTSTTQNARSSSQQLGANSLVHCLLKLAGRD----NIKEPLLKALYGLLATLALSSECRNIMFKSNFFREFLELNPRKTKKSKHVKKMEVYWLELMLNLSFSTEGQQMILKTGDSVDLLLDFVDCGSGPGLEYSVLIIRNMCCHCSSKPKLLANDKLLPLLLSC-LNKDSGKVQTIAASALLGLVFNNQKAKVMMKNANLLPK---LQEILSN 2215
BLAST of Rotatin vs. TrEMBL
Match: A0A2C9M5Y7 (RTTN_N domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106052869 PE=4 SV=1) HSP 1 Score: 204.527 bits (519), Expect = 1.243e-48 Identity = 307/1356 (22.64%), Postives = 563/1356 (41.52%), Query Frame = 2
Query: 572 DIEQENLLKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFIS--EKQYSTIAVKAISKL-CY-KMLQY-INTCLDPNFFSTKNTF------------------GIINQ--NYGEYEEDNHWN--------------------SDDLLGFHYEPNFGLPEPLPVPVFCLQVINVCLYHILEIDTDSNWAELFHLICTAIELFRESFGSDLRQPYL-LELW--------NLKDQINDVLTLWSRLL-----KQYSII-EDMNYRKLSNKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFLNNNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQL----NIQNMTDFLVSSVNDNKHERWFPRLL-NGQSKSL-DIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKILDKS-----HLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQD-----------NLSAEIKILEIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYL-RYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETSLWISTFHKFLLNIPSCSADFILLEFIMDVLENI---IKLSMTNHMKDFIQWLVQIMTTPNNSLFYFLEKGDKLIG---KTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQS------NTRSDIIKIHKMVFDICLNQLTQYLNYP-FDNL----SKLLIHIHLMSR---SLELKSNFFQGNTLKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWL--RKSP---------WLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGK-LPFPNSMEIP 4294
D + L LS P +PLT D+ V+ + TL++ D ++I +F DV+ +DFP+ +FLQR +LK LL S + I ++A L CY K LQ I++ DP+ ++ K F G Q NY ++ H DD L EP+ + L VP FC+ V+ L ++ T S L IEL +E+ +L P L + +W ++ D++ D + ++ LL +Q++I ++ + ++S R +Y+ L +F++ + + + L E K + +D+ L L ++ + + + + +S +F + + AE S+ L Y + F F F S + S++ + +C +L +LM S+ + I D ++ +L + +A PKS S FL+ ++ + FGL + + ++ + L + Q +M+ +W++ + K + LQ D T +GR + +L ++ + L +K+ L L + +R +A ++++ FLR + + +L ++ M+ L+ P+L+ + S+S+ + K+++DI+ S +D V+KSA EQ+ I L+ ++H F S+GG+ V +++ +L K + + +P+ I IL + + +R L D L +LI++ L + AD+S IL L++F+ V K D S ++I++ R LPF+ + TS + T + P++ ++I W G++ L +K F+ S+ N + D+++ LT + VL S + L N+ I A +H V +++ + L V + + W F+KF++ P+ +D LL I+ + ++ +K S+ + + WL+ + P + L K + K RRL NK L+ YL N+ L + S R +I++ + F+I + P F NL S L +H+ +R S E S +++ + + ++N +++ FH GR +S ++MG GV L HL +E+ + WL R+ P WL+ L YR ++RA I + E R ++ +C I P LW A ++ LD E +V+ +A +L NL + +P N P
Sbjct: 209 DSQDLGLFHLSTFPWLPLTQTDQQVIESTKSTLQTKDPTMLIGACEFLADVVFQDFPAELFLQRPEILKNLLSITSLPDPLDCLIVIQACKTLTCYLKCLQVRIHSFQDPSMYTLKQDFNASSSTDTSAMHSTDSSRGAYPQRRNYFDWGNQRHRGDGQDVETTPSDSRSSSVNTEPDDQLDMDDEPSL-IQHQLTVPQFCVSVLQRILP---KMHTSSKV-----LGVAVIELIQEAL--NLLMPTLDMGIWTDNRSTSRDVVDRLVDCVDCFTDLLTYHHHRQHTITPKESSLAEVSTHRILYMGLVHTLTSFLTMM---PLDMVQLPEKLKLIVPSIVYDEALALANSRYRSVMLAVAQKLGLSVPTDFQHVVDICRSLNKTCQFCMQVDAKNVSVSDLADMAEESISSLSYHLHLPFVSRFVCFISKLCSSK--AAERSVVTQCTGVLLQLMSYPIDSVRLVCYVTIADTIKKNLQVSQATDPKSQSCLLTKFLINPDVLYEITCFGLADSVAKVRSCSADSLNHLLQSQLLMTEGLWQEFLLALMKSLPVLQGYTDHTTPLGRSLWYILDPTTTTHNLPLFEKLRGS----------LRLMCLSDKKLRMDAAKHVKWFLRSETMAENKLPDNFDVDKMSSLLILPS---------PQLVEDDTSRSVFKEEGLKQVYDIFTSHPVDPGVKKSAGEQMAIMLKDPHLHAAFKSSGGVEVVCFIVQHSVLKKEENVQDNSNSNLPACIFILRYLTHYDYALRHTLAKDQPLYYSLIRVGLIHQESESIRADVSHILTLLLFDEVAKFDVSFGQSQTPGTKFSLPVQIVQ----RYRLPFKPQVHYL--TSPSAITLPD----PSKDIMTTSGPSEMMKITWNCAWQAGIDQLLDSFKTPFF------SETNKEFSDQLI-------LTPVEREVLEISHIPWCLKSNILTIQQATDHSTVMAAVDRMMSYTTA-----LPVPLVIQYFSNQEWFLVFNKFMMVTPTSQSDQSLLLHILSFMNSVLHCLKPSVGSPCP-ALAWLMDKIYQPEGPMISLLNKPGLPVEPEYKNTFRRL----NK----------ELLNYLTSVNLSLPYQLSYRVKPLQLRGEIVRTLQRGFNIG--------DAPHFYNLASLESSLFCLMHITARPGWSCECGSM----DSISLCNQLLNSLLEVVWSFHIGRGGASRSYMGKGVTKS-----CSLCLRHLAYEMITKSDDPGWVKSWLYTRQDPSNGVDQGLNWLLTLWAYRDPEVRAAGLGIAVALTSTEAGRLLVNSNCKHI-PGGLWGAAFSILLDPLECCVVQQQAALLLINLTSQTMPSGNVETTP 1468 HSP 2 Score: 102.834 bits (255), Expect = 1.129e-17 Identity = 105/377 (27.85%), Postives = 179/377 (47.48%), Query Frame = 2
Query: 5300 ESLSRINAKSHSYTDTSINERINVHNTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTVKLIFLLLSNLVWCPEGINILLRIRFLDKFEQINPNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLS-----TLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQL-SLSTLKALLYSNNKIRVYLKSANLVTKLMSL 6412
ES+ +IN + + S ++ V + K LL SQ K + + G +E +K H+ L + V V R+ ++E++ + + L N + + N + G ++V +LW D+ LL IL+ L SA + A ++ + + ++++ + +L +L +KL F LL+NLV E NILL+ FL++F Q+NP + ++S +AI LW L+ +LSF+ +GQ I+K I VL V ++L + + LIIRN+C H K KL+ LI + +D+ KQL ++S L A +Y+N+K RV +K AN++ +L+ L
Sbjct: 1864 ESVIKINVEEKGTKEAS--RKLKVISAFKSLLAISQSAKEFALDAGLVEVALDYLKQTHASLSLETLDMVKVGGRKEG-------------PYLQELVLMFDFLHNLMFNNANVKVTCYHGGLIVVVQKLWAWCHQDTSLLSSILSLLTVYSAHCNSAAGSL----SLPMSAQSSTKPSGQLTILHGVLKLAQRELDRDECSP-ILKLTFSLLTNLVMNAECRNILLKSTFLNQFSQLNPRR-KRSKVRVAIDMLWCDLLTALSFSVDGQQIILK-----IPDVLTVLLDMLEAGSTHCQHCSTLIIRNLCCHSTNKPKLLAV-DRLIAVLMDQMDNTSDEKKQLVAISALWAFIYNNHKARVQVKLANVLPRLVDL 2213
BLAST of Rotatin vs. TrEMBL
Match: A0A1S3GZK1 (rotatin OS=Lingula unguis OX=7574 GN=LOC106150846 PE=4 SV=1) HSP 1 Score: 194.897 bits (494), Expect = 1.178e-45 Identity = 287/1367 (20.99%), Postives = 544/1367 (39.80%), Query Frame = 2
Query: 539 NENSNFMKEIADIEQENL-------LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI---SEKQYS---TIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGII-----------------------------NQNYGEYEEDNHWNS--------DDLLGFHYEPNFGLPE-------PLPVPVFCLQVINVCLYHILEIDTDSNWAELFHLICTAIELFRESFGSDLRQPYLLELWNLKDQINDVLTLWSRLLKQYSIIEDMNYRKLSNKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFLN-NNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVND---NKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKILDKSHL-----SILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEI-----------KILEIIGDRILLPFR------VGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNY---LRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQV-QXXXXXXXXXXXXRNHAFLNVDIYDIFNETSLWISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIE--SRL--PNFFLLLMRYLN--LNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQGNTLKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDW---------LRKSP-------WLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSMEIPIDVNQN 4312
+++ F ++A + +E L +K S P +PLT D++V+S + +L S D +VIS +F +DV+ DFP+ VFLQR ++K+LL+ + SE S T AVK + LC + I+ DP ++ K FG NQ G+ + + +S D +G H + + + E L +P CL+ + +L D ++ L+ ++++ S + + ++ +++ + L + + Y + R +L + F+ + E A + EH + FD+ L L + Y+E D + ++ ++ S + +F LN N + A + I AE+SL L Y + +E+ S + S + ++L E++ + + F+ +V+++L I+ KP S KI FLL ++ + FG+ + + + A + L + Q +M IW ++M I M LQ L + +G + S + + F+ L + ++K+ R EA L FL + + + + T+ V+ ++D + R+ + +D+ K+ +I S ++DI V+KSA +QL I LQ ++H F GGL VL+ +K +L ++ +I +P+ + +L +V +R L +S +++ ++ ++Q D S +L L++F+ V D S + + + R +PF+ + ++ + S T+ P Q E L+I W + HG + + LR K + + KL LT +D +L + + L+ + NA +H +V Q+ + + R H +Y + W +FL IP+ S+D LL I+ L +++ + WL ++ +L +I D R+ + ++ +RL + R++N I +S ++ R DI++ ++ + L Q+ N P +L L + ++ S ++ + ++N +++ FH GR S+++MG GV L HL +E + + DW L + P WL+ L YR ++RA I + E R + HC +++ +W A ++ LD E +VR +A +L NL S+E+ ++ Q
Sbjct: 191 DQDGQFAGQVAGVTREQLDRDISGCIKFSTFPWLPLTPTDRHVMSSTENSLHSGDQTMVISACEFLSDVVFLDFPAEVFLQRPSIVKILLQLLQVPSEGGTSGVVTAAVKTLRDLCVHLQHRIHFYQDPTLYTPKIDFGASPPSSVASPSNQTLNSSDTRPSVIGRSDTRNQGDGQDRDTSPSDSSRPVSPQEDQDMGNHQQEDTDVDEMDALQFSQLTLPQCCLRTLTRA-APLLRTDRKDTLVDVALLMDESLDVLGLSLTGQVWTDGSITARDMVEKLQEFLQILGE-VSSYHHHQSGTSGDKEQHRLGFLVVSVFLHKFLKLLVPVEQAQSVISEHLSQSVSWIVFDEALSQSLPALRPAFLPYLEAVDGEGYQRFLQAETIVVSMQKTCQFLLNCQNRCDQDATELIQIAEDSLPSLPYHCNLTLVEEYVRLCSDVCSP--VNASPIVMTNSRKVLLEVLAHSLVEVKLRAFSVCLALVKDTLHIDGPGKPSSC--QKILFLLDRHVLYEICCFGMDDANERVLGVAQQVVLYLLQSQLLMPDHIWSRLMQIVAAIMPILQGYAGLQSPLG----SSIRSLCEVTPTPVKGTLPKLERFRG----VLRMMFSKDVRTRAEALSKLAWFLSDEE--DSEKKLPVFTELDVAGLSDLYVIREPRYLEDSDEARRSVFQVDSMMKVVNILRSPSVDIGVKKSALDQLAIMLQDTSLHKVFREEGGLEMVLDSMKTAVLKENSTVQFSETIFLPACVTLLRHLVHSDYHLRHQLAHESQTYYMIVRASILHNKNEQVHYDASHLLLLLLFDEVASIDVWSVAVDSSPKKSVQCFSLPAFMAHRYHIPFKPITHHVISQHRIDLVSSTD---------PLQTGPPAEM----LKIAWNVAWHGDMESLMVDLRKKKEEEKETEFSA---------------KLHLTPADRAILETTHLISAIQQCLYSMCNATSHTEVSQALGRLKTYMTVCRVHGLGAEILYRLK-----WREAIGRFLQVIPASSSDESLLMEILSFLNLMLEAGPVPQAEQV--WLGDLLVKKKGAL-------PNMISLQDGRQQDGEGDHVDDITRLLRKEVLCFVRRFVNRLPQMIAKTSLKTWLRGDIVR--SLLLRLNLADAPQFYNLP--SLESTLPCLTNVTARPGWSSGCKDTDSSTLCLQLLNCLLEVLSAFHIGRGGMSMSYMGRGVTRN-----ATLCLRHLAYE----MSDQSTEEDWPKCWLFTKQLPEHPSTDPAYNWLIPLWTYRDPEVRAAGLGIAVALTSTEAGRITMTTHCQQLI-GGVWGAAFSVLLDHYECSMVRQQAALLLVNLTSDTMPSGSVELGANIWQG 1485 HSP 2 Score: 89.3521 bits (220), Expect = 1.238e-13 Identity = 138/654 (21.10%), Postives = 284/654 (43.43%), Query Frame = 2
Query: 4592 NISTPLMISALVSFLCNLYYYLPDQVSSQFNELRINTLIMNMFDPHTLKVYMESFQTEHYSTT-------------------VDLVLAFTNCVKLIRLQINMNSSLKDSFLIDEKFIETIIQLLI--VTKPTSDFDESHDNRLMKLDLYKYISQLRVEILYLLISLVIPTPIKNHVQLMLAHV------ASHVTGIFNCIKEMIEIESRKSGNVALCFVSVFLCLSS--SESKFNSL-----IRSLESQRFSNSERVIIED-------FTELLLKKRESLSRINAKSHSYTDTSINERINVHNTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXI-SLIENICRLTSAD---LTAYYLRENTVKLIFLLLSNLVWCPEGINILLRIRFLDKFEQINPNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHC----LDSN-HICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKL 6403
+I+TP ++SA+ L NL +P + + ++++ DP L VY Q ++ V LV +T+ ++L+ +++ +++ L + ++ ++LL +T P + + L+ + LISL+ + + + + A V A + IF+ ++ + AL F++V LC ES L I S+ + +E+ ED ++ L+K +++ N H T ++ L+ LL K + G +E ++ LH++L+ ++ + G V + K +++ ++ LELL++F ++ ++ A G +++++W +D L++ +L+ L N +A + A ++ + + + SL+ + +LT+ + L+ L + V+ +F +LSN+V E I+ + F +F + P + K+ A+ +WL L+++LSFT +GQ I+K S + + ++ S L+++RNMC H K K++ N E + C L++N + +++S L AL+Y+N K + LK++N++ KL
Sbjct: 1621 SIATPSLVSAVSQLLTNLVVLMPQDTLDCLKKEGLLRALVSLTDPDLLAVYTRDMQQSKTPSSSPSSGSSEDEGMLRQLVILVGLVEMYTSILQLVTHCCELDTEVREEILQEAVILDGTLRLLEYNITDPI---------------VIEKCQALQTAVYNFLISLLQHSGQQGFLNIAQACVKQWDILAGSLAKIFSGSPSEVQ-------DAALNFITVLLCEEGKIQESYVKKLGHKKSILSMLDKTGGKNEKG--EDSPTSGAILSQALIKLYDTVVVKNVTVHLEARTR-----HISVALRSLLSQCATAKMAALEAGLMEILMEHLRKLHNQLN---MESLQI------GKVTSGRKKDD--PLLESMVLTLELLRSFMHDQAEVKTAAQLSGLSSVLHQMWGWCHVDQALMNAVLHVLANYTAKCLAAASSLALTSALSSVDLPNAKPQHNNNSLVHCLLKLTTRECERLSKTSL--SMVRTLFAILSNIVLSSECRGIMWKSNFFHRFSSLCPLKPSKTKVQQALDVMWLELLVNLSFTTDGQQMILKMGDSLALLLEFCESEHSQAASSALMVLRNMCHHSANKPKILGN-----EKVVPCLLRYLETNLDVGDATVAMSALWALVYNNQKAKATLKNSNMLRKL 2227 HSP 3 Score: 67.3958 bits (163), Expect = 6.300e-7 Identity = 37/113 (32.74%), Postives = 66/113 (58.41%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNLLKV 387
L KL H L+E+R RAL+N+ +K++ II DL + K + LLEWFN + D V+ L+ ++ + + IGG+EFLTQL++ + ++DQ+++ ++++
Sbjct: 11 LQPLFRKLGHQLEEIRVRALRNVQSKLDHHIICDADLVQEKHLLIRLLEWFNFPTCPHQDEVMTLLARLAKHSSAAQLLQDIGGVEFLTQLRVNCDKVLHPLIDQVLEYMMRL 123
BLAST of Rotatin vs. TrEMBL
Match: A0A1S3K5M3 (rotatin-like OS=Lingula unguis OX=7574 GN=LOC106178910 PE=4 SV=1) HSP 1 Score: 188.734 bits (478), Expect = 5.714e-44 Identity = 294/1341 (21.92%), Postives = 537/1341 (40.04%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI---SEKQYS---TIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQNYGEYEEDNHWNSDD----LLGFHYEPNFG-------LPEPLPVPVFCLQVINVCLYHILEIDTDS----NWAELFHLICTAIELFRES--FGSDLRQPYLLELWNLKDQINDVLTL------WS-----------------RLLKQYSIIEDMNYRKLSNKRQ---IYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFLN-NNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVND---NKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKILDKSHLS-----ILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEI-----------KILEIIGDRILLPFR------VGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNY---LRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQV-QXXXXXXXXXXXXRNHAFLNVDIYDIFNETSLWISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIE--SRL--PNFFLLLMRYLNL--NNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQGNTLKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDW---------LRKSP-------WLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSMEIPIDVNQN 4312
+K S P +PLT D++V+S + +L S D +VIS +F +DV+ DFP+ VFLQR ++K+LL+ + SE S T AVK + LC + I+ DP ++ K FG + + NS D ++G N G P PV + ++ + + D D +++L C L R + +D R+ L+++ L D+ DVL L W+ ++L + S +K Q +L + F+ + E A + EH + FD+ L L + Y+E D + ++ ++ S + +F LN N + A + I AE+SL L Y + +E+ S + S M ++L E++ + + ++ +V+++L I+ KP S KI FLL ++ + FG+ + + + A + L + Q +M IW ++M I M LQ L + +G + S + + + F+ L + ++K+ R EA L FL + + + + T+ V+ ++D + R+ +D+ K+ +I S ++DI V+KSA +QL I LQ ++H F GGL VL+ +K +L ++ + I +P+ + +L +V +R L +S +++ ++ ++Q D+S +L L++F+ V D S + + + R LPF+ + ++ + S T+ P Q E L+I W + HG + + LR K + + KL LT +D +L + + L+ + NA +H +V Q+ + + R H +Y + W +FL IP+ S D LL I+ L +++ + WL ++ +L +I D R+ + ++ +RL + R++N I +S ++ R DI++ ++ + L Q+ N P +L L + ++ S ++ + ++N +++ FH GR S+++MG GV L HL +E + + DW L + P WL+ L YR ++RA I + E R + HC +++ +W A ++ LD E +VR +A +L NL S+E+ ++ Q
Sbjct: 10 IKFSTFPWLPLTPTDRHVMSSTENSLHSGDQTMVISACEFLSDVVFLDFPAEVFLQRPSIVKILLQLLQVPSEGGTSGVVTAAVKTLRDLCVHLQHRIHFYQDPTLYTPKIDFGASPPSSVASPSNQTLNSSDTRPSVIGRSDTRNQGDGQDRDTSPSDSSRPVSPQEDQDMGNHQQEDTDVDEMDALQFSQLTLPQCCLRTLTRAAPLLRTD-RKDTLVDVALLMDESLDVLGLSLTGQVWTDGSITARDMVEKLQEFLQILGEVSSYHHHQSGTSGDKEQHRLGFLVVSVFLHKFLKLLVPIEQAQSVISEHLSQSVSWIVFDEALSQSLPALRPAFLPYLEAVDGEGYQRFLQAETIVVSMQKTCQFLLNCQNRCDQDATELIQIAEDSLPSLPYHCNLTLVEEYVRLCSDVCSP--VNASPLMMTNSRKVLLEVLAHSLVEVKLRAYSVCLALVKDTLHIDGPCKPSSC--QKILFLLDRHVLYEICCFGMDDANERVLGVAQQVVLYLLQSQLLMPDHIWSRLMQIVAAIMPILQGYAGLQSPLG----SSIRSLCEVTPSPVKGTLPKLERFRG----VLRMMFSKDVRTRAEALSKLAWFLSDEE--DSEKKLPVFTELDVAGLSDLYVIREPRYLEDSDEAGRSVFQVDSMMKVVNILRSPSVDIGVKKSALDQLAIMLQDTSLHKVFREEGGLEMVLDSMKTAVLKENSTAQFSETIFLPACVTLLRHLVHSDYHLRHQLAHESQTYYMIVRASILHNKNEQVHYDVSHLLLLLLFDEVASIDVWSVAVDSSPKKSVQCFSLPAFMAHRYHLPFKPITHHVISQHRIDLVSSTD---------PLQTGPPAE----MLKIAWNVAWHGDMESLMVDLRKKKEEEKETEFSA---------------KLHLTPADRAILVTTHLISAIQQCLYSMCNATSHTEVSQALGRLKTYMTVYRVHGLGAEILYRLK-----WREAIGRFLQVIPASSLDESLLMEILSFLNLMLEAGPVPRAEQV--WLGDLLVKKKGAL-------PNMISLQDGRQQDGEGDHVDDITRLLRKEVLCFVRRFVNRLPQMIAKTSLKTWLRGDIVR--SLLLRLNLADAPQFYNLP--SLESTLPCLTNVTARPGWSSGCKDTDSSTLCLQLLNCLLEVLSAFHIGRGGMSMSYMGRGVTRN-----ATLCLRHLAYE----MSDQSTEEDWPKCWLFTKQLPEHPSTDPAYNWLIPLWTYRDPEVRAAGLGIAVALTSTEAGRITMTTHCQQLI-GGVWGAAFSVLLDHYECSMVRQQAALLLVNLTSDTMPSGSVELGANIWQG 1279
BLAST of Rotatin vs. Ensembl Cavefish
Match: rttn (rotatin [Source:NCBI gene;Acc:103043851]) HSP 1 Score: 72.0182 bits (175), Expect = 9.393e-12 Identity = 73/346 (21.10%), Postives = 157/346 (45.38%), Query Frame = 2
Query: 1805 FDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNII--------KFLPLFYNKNYDVRKEACQYLEQFL-RFDRLWNCQ--LNIQNMTD----FLVSSVNDNKHERWFPRLLNGQSKS-LDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQD 2794
++V+ L ++ AKP S+ ++FL + + + FGL ++ +E++ A + L +G+ +M+ W + + + LQ +G IL IS Q + + L L +NK + VR A ++L L +R+ NC+ L+ ++ ++ + D K L+ S+S L +D+ K+++I S +D+ +R+SAAEQL + LQ MH+ + G ++ + + + ++ L+ T+ +L ++V +R +L S + +L++ +L ++ V + + ++ ++F+ + + D
Sbjct: 624 LNVVKECLGVQNVAKPASSVCAGVHFLFHSRVLYEIGTFGLQDQAREVNSAAKDVLLYLLKGRLMMTASSWTRFNEALYPVIPVLQSYAGSTDALGNCILL----------------ISGVSGDQGDGVCSPAGRLRAALRLLFNKQHTVRSTAVEHLLHHLTSSERIKNCRPVLDKTALSSLPSLYVTNKCVDIK--------LDDASRSFLKVDSVTKLYNILASETVDMVLRRSAAEQLVVVLQDSAMHSVLKTQGLPDKLISFITESV-NSLNVDCLLEPTVGMLRKLVYADPSLRHSLAQQSDFLIHLLRASLLIKANQGDVTEAAVLMCFLLFDEIARVD 944 HSP 2 Score: 61.6178 bits (148), Expect = 1.086e-8 Identity = 43/124 (34.68%), Postives = 66/124 (53.23%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNL--LKVNSNDTILSE 414
L+ + K H L E+R RALKNI +K++ +I+ DL + + LLEWFN V + VL+L+ S G + +G +EFLTQL S I+D I +L L V+ + +++ E
Sbjct: 3 LSALIKKTAHSLVEIRVRALKNIRSKLDHGLISRPDLVQERTLFINLLEWFNFPEVPMQEEVLELLSTLSTHPTGAHMLSDVGAVEFLTQLSPSVEPSLQTIIDSIFDHLFHLPVSPSPSVILE 126
BLAST of Rotatin vs. Ensembl Cavefish
Match: rttn (rotatin [Source:NCBI gene;Acc:103043851]) HSP 1 Score: 71.2478 bits (173), Expect = 1.642e-11 Identity = 73/347 (21.04%), Postives = 157/347 (45.24%), Query Frame = 2
Query: 1805 FDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNII--------KFLPLFYNKNYDVRKEACQYLEQFL-RFDRLWNCQ--LNIQNMTD----FLVSSVNDNKHERWFPRLLNGQSKS-LDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKILD-KSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQD 2794
++V+ L ++ AKP S+ ++FL + + + FGL ++ +E++ A + L +G+ +M+ W + + + LQ +G IL IS Q + + L L +NK + VR A ++L L +R+ NC+ L+ ++ ++ + D K L+ S+S L +D+ K+++I S +D+ +R+SAAEQL + LQ MH+ + G ++ + + +++ L+ T+ +L ++V +R +L S + +L++ +L ++ V + + ++ ++F+ + + D
Sbjct: 639 LNVVKECLGVQNVAKPASSVCAGVHFLFHSRVLYEIGTFGLQDQAREVNSAAKDVLLYLLKGRLMMTASSWTRFNEALYPVIPVLQSYAGSTDALGNCILL----------------ISGVSGDQGDGVCSPAGRLRAALRLLFNKQHTVRSTAVEHLLHHLTSSERIKNCRPVLDKTALSSLPSLYVTNKCVDIK--------LDDASRSFLKVDSVTKLYNILASETVDMVLRRSAAEQLVVVLQDSAMHSVLKTQGLPDKLISFITESVNSLAANVDCLLEPTVGMLRKLVYADPSLRHSLAQQSDFLIHLLRASLLIKANQGDVTEAAVLMCFLLFDEIARVD 961 HSP 2 Score: 64.6994 bits (156), Expect = 1.367e-9 Identity = 45/131 (34.35%), Postives = 69/131 (52.67%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNL--LKVNSNDTILSENEGFSCS 435
L+ + K H L E+R RALKNI +K++ +I+ DL + + LLEWFN V + VL+L+ S G + +G +EFLTQL S I+D I +L L V+ + +++ E + S S
Sbjct: 3 LSALIKKTAHSLVEIRVRALKNIRSKLDHGLISRPDLVQERTLFINLLEWFNFPEVPMQEEVLELLSTLSTHPTGAHMLSDVGAVEFLTQLSPSVEPSLQTIIDSIFDHLFHLPVSPSPSVICETQFLSSS 133 HSP 3 Score: 55.4546 bits (132), Expect = 9.681e-7 Identity = 32/110 (29.09%), Postives = 56/110 (50.91%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLK------LLLRFISEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTK 904
LK SV P + LT D+++LS + +LRS+ +V + + DVI++DFP+ +FLQR +++ + A+ + +LC + + DP+F S K
Sbjct: 169 LKFSVFPWLALTPTDRHILSSNESSLRSESQDLVRTTCELLQDVIMQDFPAEIFLQRPRIVQNLLLLLNVSSESDSSSLPLRALACLQQLCRNLRSRLRFHRDPSFCSAK 278
BLAST of Rotatin vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007193.1 (pep scaffold:Pmarinus_7.0:GL476541:87500:145477:-1 gene:ENSPMAG00000006436.1 transcript:ENSPMAT00000007193.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 114.39 bits (285), Expect = 2.158e-25 Identity = 200/862 (23.20%), Postives = 345/862 (40.02%), Query Frame = 2
Query: 1814 VQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKE---IHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVNDNKHERWFPRL-----LNGQSKSL-DIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELL-KGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSA--EIKILEIIGDRILLPFRV-GKYSANKTSKTEWTFSNFSKIPNQKWN--YQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLN-NSIPQFI-LCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETSL----WISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMT-TPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLEL-----KSNFFQGNTLKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLRKSP------------WLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNS 4282
VQ SL I K S + + FLL + + L FGL + KE + A A+ L + + +M W Q++ + LQ +G I+ L + S + + + + + L L K VR Q L L + L +V + P + G KS+ D K++ I++S +D++V+KSAAEQL I +Q +H G + V+ LL + D L +V + +L R+V H +R L L+ +L+++ L + + ++S ++ L++F+ V + A L I + + LP V ++ T S IP + +R+ W L H G+ L+ + + ++ P + L + SD+ L +P + LC L + A +H + + +L+ + L L+ N A L + L W ST +FL+ P +AD LL ++ L+ ++ +QW++ ++ +N L L K + + + + + + L +FF L+R L + KQS S+ ++ H + + L +L + + F L L + M+ + L S+ Q N L++ + +++L F GRE + ++F+G GV + L +L D + + V W RK WL+ L R +++R S + + + EQ L C I LW AL + LD E+ +VR EA SILQNLL LP P S
Sbjct: 568 VQESLGIHNVTKSVSTACLGVRFLLHSRVLYELCAFGLQDSHKENVKVFLAARAIVLYLLQSRLLMCAATWGQLLEALTPVLPVLQGFAGEEGALGSAIVNLSETESQSGEGPIPRT--------DRLRASLRLLMAKQLPVRSAGVQQLAWHLVTEE------QAGQKRPHLPGTVLAGEPNLLIPDVPLCLDTKGIGKSVFKEDAVCKVYGIFMSEAVDLAVKKSAAEQLTIMMQDTILHGVLKKQGAVEKVISLLHQAAHKDGRGLECMVRPCLTLLRRLVYHDPCLRHRLAYQPTLLLSLLRVLLVYQSEAVVQLEVSVLMSLLLFDEVAHIEKWQALHHSLALNIKLEPLSLPLVVEQRFHLPLVMATHHVVSPHRVIPPPPSDPLVAPPARDAIRLGWNLAWHQGVEAMLQAHAA----------SQLEATNQGFP--ELLRMNGSDVMGLRLTHLPSSLQLC--LEAVSRAASHGEAKLALLRLRLQLL--NDALLPQPGAESKAAQMLLSMDWRSTLKRFLVVRPVSNADEKLLGEVLAFLQALLHAQGDIPDHSMLQWILLLLKEQESNHLLDLLNKSNTQVDARHTVTESPTRKGLLKELSSFFNALVRALGCS----PDKQSLAASNGVRCH--LAALLLGRLKEADSSGFYELPSLEATLRGMAHATALPGWSVASSATQSNNLRL--QYLASLQEIVLSFSIGREGNGLSFLGKGVTKSSVVCLRQLASEMSDQAEN---QDWVSPWSWSRKGSGRGETAAQLGLTWLISLWVDRDLEVRLASLGLGTALTSEEQGCMALVAACQNI-SGGLWGTALKILLDQYESSMVRLEAASILQNLL-VLPMPAS 1386 HSP 2 Score: 57.7658 bits (138), Expect = 4.115e-8 Identity = 37/111 (33.33%), Postives = 58/111 (52.25%), Query Frame = 2
Query: 593 LKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFI-------SEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTK 904
LK S P + LT DK+VL+ + +LRS + ++ S F DV++ DFP+ FLQR +++ LL + Q + A++ + LC + + DP FFSTK
Sbjct: 95 LKFSTFPWLSLTPNDKHVLTSTERSLRSKNASLIRSSCDFLLDVMMCDFPAETFLQRPNIVENLLLLLRLAGGADGTHQLALHALRCLHHLCRNLTSRLCFHRDPAFFSTK 205
BLAST of Rotatin vs. Ensembl Nematostella
Match: EDO36600 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SI71]) HSP 1 Score: 93.5893 bits (231), Expect = 8.939e-19 Identity = 98/364 (26.92%), Postives = 171/364 (46.98%), Query Frame = 2
Query: 5381 LKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQ----IIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLREN----------TVKLIFLLLSNLVWCPEGINILLRIRFLDKF---EQINPNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNG--SGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKLMSLR 6415
L LL SQ K ++ G +E+T +IK++H +L N+D ++ +G SWK+ +I E+++ L++L+N Y+ + + G +Y+LW ++S L+ +L L L+A A I + + S + + N+ + T++ L N T K +F LL+NL E I+ + FL +F E + P Q + A+ WL L+++LSF +GQ +MK G + +++ +V ++ LL++RN+C H K L+ N LI+ + LD ++ L+ S L +L+++ K RV LK+A LV KL +R
Sbjct: 1278 LTSLLAVSQTSKASALKDGLVETTLDQIKHIHVQL------NMD-SLQLGRGP--------SWKKRENPLIDEMVACLDVLRNLVYQYEEGKLACMESGLIGTLYKLWAWCTVESNLMSAVLLLLSTLTARCPKVAACIAGGSSI------SRASQSGTTFLNNLLK-TASSTNRKILSSNSCPDLDTHLATHKALFTLLANLAMSAECRGIIWKSNFLQQFSSLEPLKPIQGRPKHFSRAVGGAWLSLLVNLSFHTDGQAMLMKVPGCVNVLLEFCHVTHSKQANSELALLVLRNLCFHGNTKAVLLSN-DDLIQLLIGQLDQLSDHVRTLAASALWSLVHNCQKARVSLKNAGLVKKLEEMR 1618 HSP 2 Score: 55.0694 bits (131), Expect = 4.161e-7 Identity = 87/419 (20.76%), Postives = 165/419 (39.38%), Query Frame = 2
Query: 3125 DLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETSL-------WISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIH------KMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQG---------------NTLKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLRK-----SP--WLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLW---SVALTLYLDISEAPIVRSEAISILQNLLGKL 4267
D ++L +S + D L + A +H + ++ + R H ++ + ++ ++ W ++L +P+ + D LL I+ VL +I+ + + W+ +++ L L S +T ++ + L F L++ L ++ +N R D +++ + F++ L QY + P S L H+ SRS E + QG + +++ S+++ ++ FH GR S +FMG GV + L HL E+ L WLR P WLV L RS ++R M +I S ++ E + L + L W +V LT+ + + +VR +A +++N + L
Sbjct: 399 DSHILRSSYTTTCIADQLTTLQRAASHHPARRAVGVV------RTHLITDLGLAGRGSKLAVETVYTLQWQEALERYLQVLPNNTEDDELLLEILSVLSDILTSPSPP-PEPVVAWMARVVINNEGPLLTLLRMPQNDSAHPTSEAVTDSRRTLRKTLLAFLSTLVKALLRSS-------ANWRFDSERVYIGDLAYAVAFNLDLADNPQYYDLPVLE-SALECLGHVTSRSHE---HSRQGQEPYGDVQYHVSECKSPMEMCSSMLRSLIQVVTAFHAGRLGSRSSFMGKGVTIHAC-----LCLTHLAREMQILDPDKSWAEHWLRARDTGDQPLNWLVTLWSDRSPEVRWMGLSIASTLVTCEAGQGGLV----QALQGDAWAVFAVGLTILMSDGDCGMVRHQAAHLVRNFVRTL 790
BLAST of Rotatin vs. Ensembl Nematostella
Match: EDO36601 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SI72]) HSP 1 Score: 71.633 bits (174), Expect = 3.109e-12 Identity = 43/113 (38.05%), Postives = 68/113 (60.18%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNLLKV 387
L F+ KL+H ++E+R RALKNI K+E +I + DL + +E + LLEWFN VL+L+E S+ + ++ IG IEFL+QL+ + +Q +D +I LL++
Sbjct: 24 LQQFILKLSHQVEEIRVRALKNILFKLEHKLICMSDLVQERELLIRLLEWFNSPSAPLKKEVLELLEKLSKHVSAAQILLSIGTIEFLSQLRQDISPDLYQDVDNVIHQLLRL 136 HSP 2 Score: 52.7582 bits (125), Expect = 1.824e-6 Identity = 38/152 (25.00%), Postives = 68/152 (44.74%), Query Frame = 2
Query: 521 PLSKNRNENSNFMKEIADIEQENLLKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFISEKQYST------IAVKAISKLCYKMLQYINTCLDPNFF----------STKNTFGIINQ 928
P N + S D Q L+ + L+V+D++VL+ + L+S +V +F +DV+ +DFP+ +FLQR V + LL + + +T + ++ LC + + + DP F S+K T G+ +Q
Sbjct: 217 PSQSNMPQGSLSTSRSHDTAQLESTALTFFRWMSLSVSDRHVLTATDSRLQSRSPTLVAQSCEFLSDVLFKDFPAEIFLQRPSVFRTLLDILDTSRNTTDDTLLLSTLNCLTSLCQHVQRRLMATSDPALFCPDNDCWKRTSSKPTAGVPSQ 368
BLAST of Rotatin vs. Ensembl Medaka
Match: rttn (rotatin [Source:NCBI gene;Acc:100302007]) HSP 1 Score: 97.8265 bits (242), Expect = 1.375e-19 Identity = 217/1045 (20.77%), Postives = 421/1045 (40.29%), Query Frame = 2
Query: 1793 FTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSV-NDNKHERWFPRLLNGQSKSL-DIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRVGKYSANK-----TSKTEWTFSNFSKIPNQKWNYQE--SFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDN-YDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFN--ETSLWISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDF----IQWLVQIMTTPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLP--------NFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQGNTLKVISNVINLCGDLILKFHTGREESSITFMGSGV----VYYLLRILIELICNHLDFEVDRL----IETTVCQTDWLRKSP----WLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSMEIPIDVNQNDENEDISSLFIDRNSSGKYQEINGFYQKLLYLDFFRKAGLSLTNFYT------LPF-------------INCSYFKHKP--------LYDPE-----YQINFDSDFQKSVNINQILSQMRTACEINISTPLMISALVSFLCNLYYYLPDQVSSQFNELRINTLIMNMFD 4723
+ ++V++ L I+ ++ + + +NFLL + + + FGL + +++ A + L +G+ +M+ W + + LQ + +G +L + ++ + + +SN + L L ++K VR A Q + + + + +M +++S+ N + L+ +SKSL +++ +K+F + S ++DIS+++SAAEQL + LQ MH S G V+ L+ G + L+ IL ++V +R +L +LL+ L++ +L +K ++I+ ++ L++F+ + S+E+ + + D + PF + +A + T S + +P + + L++ W H G+NN L +G + SD N Y+ + L L+ES L + + L D + I A H V S L + L L++ A +V Y+ + ++ W + +FL P+C D LL I+ L K + D ++W+++++ ++ L G + S + T ++++R+ +FF L+ L+ +L + + + +++ + ++ ++ P +L + L + ++ S+ + S ++ ++I F+ +S++FMG GV V LL + E++ + + + L E T T+ SP WL+ L R ++R S + + + L R C I LW L + LD E +VR EA ILQNLL M +P + + + D S + L + +F+ S T Y LP ++ H P +P Q D+D SV + SQ+ I TP +++A L NL LPD V + + ++ L+ N+ D
Sbjct: 618 YERTLNLVKDCLGIQNVSRQEPAACAAVNFLLHHRVLYEISTFGLQDSAEKVSLAAKDILLFLLKGRLMMTASAWARFTEALHPVIPILQGYANTEESLGNCVLLISDTSDAGV-------LSNTAKLK----AALRLLFSKKPTVRMAAVQQILPHITSGE--DASTSRPDMDQSVIASLPNIFCGKNLVDVTLDTRSKSLLKVESVEKLFHLLSSDSVDISLKRSAAEQLSVVLQDTTMHPVLKSLGITDKVVSLMMGSVHGNKSFDCLLEPCACILRKLVYADPSLRHSLAQRNLLLLWLLRASLILKENKGNGSEIAVLMTLLLFDEIA-----SSEMWLEKANTDVKISPFSLPLTAARSHNIPFQAATHHAVSPYRCLPAPSDDLLALIPARQALQVAWNTAWHSGINNLLE---KLHG---VASDANEYEFNPD-------LKLSESQLLSVRVAHLPSALRDCVQAIATAAGHRSVASGLSGLSLYLLIDRLALPHVPTYNCRDTLQSLSWHTALARFLQVRPACIEDERLLVGIVTFLNAYFKRVHAEFVPDEEDRDLRWILELLLNQETAVLLHLLHG---VETQSSNQSTEQPEELKNRMSQTLQRELISFFNTLLLRLSHTTDRLCLASAGLFKSQLAV-RLLQSLRVSDAPRFYGLP--SLERTLQGMVSLTAQPGWSSHCPDLEPSSLCSKYLSGLLEVISSFYVEWGGNSLSFMGKGVTKNAVICLLHLSHEMMTENKEKDSIPLWYLGSERT---TEDAGASPLGLAWLIPLWVDRDQEVRFASLGLGAALSSVASGCQALCRSCQNI-SGGLWGTLLNILLDQQETSMVRREAAFILQNLL-------VMPMPANAEEAKNSHWQHPCVHDEVSGISLVGLPALQALLHHCQYFQNVAFSATTCYRGRHTFHLPPCFDEPSGPGLKSDVSGGSGSHTPNISSAVSIADEPPACRLTAQGQSDTDTSNSVTSQE--SQLSAVEATLIVTPDLLTAHCGVLTNLLAILPDFVLTAIRQNQLLHLLANLVD 1612 HSP 2 Score: 62.7734 bits (151), Expect = 6.182e-9 Identity = 33/91 (36.26%), Postives = 54/91 (59.34%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQL 321
L+ + K+ H L E+R RALKNI K++ +I++ D+ + K YLLEWFN V + VL L+ ++S+ + + +G ++FLT L
Sbjct: 3 LSSLIKKIGHSLVEIRVRALKNILCKLDHSLISVNDIVQEKMLFVYLLEWFNFPEVPMKEEVLGLIYLSSKHPSAAQMLRDVGAVDFLTSL 93 HSP 3 Score: 56.225 bits (134), Expect = 5.098e-7 Identity = 37/116 (31.90%), Postives = 60/116 (51.72%), Query Frame = 2
Query: 578 EQENLLKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLK------LLLRFISEKQY-STIAVKAISKLCYKMLQYINTCLDPNFFSTK 904
E LK SV P + LT D+++LS + +L S + +V + + DVI++DFP+ +FLQR G++K L E Y + A+ + +LC + + + DP F S K
Sbjct: 149 ESVRCLKFSVFPWLALTNTDRHILSSNESSLGSTNPNLVRTTCELLCDVIMQDFPAEIFLQRPGIVKNLLSLLRLSSCKGEVNYLRSPALSCLRQLCVGLRRRLRFHQDPGFCSAK 264 HSP 4 Score: 51.9878 bits (123), Expect = 9.988e-6 Identity = 91/358 (25.42%), Postives = 155/358 (43.30%), Query Frame = 2
Query: 5381 LKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTVKLIFLLLSNLVWCPEGINILLRIRFLDKFEQI----NPNQLQKSASGI-AIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKLMSLREIYENDSK 6439
L LL S +N+ + G I+S ++K +S+L+S V K+S K++ + L+ F ++ + A V+ LW LLD + L L +A+ A+ + S S+ SL+ ++ +LTS L++ +N L F LL+NLV + IL + FL F + P A G ++ LWL L++SLS E+GQ +I+K GS L + ++ R LLI+ N+C K ++ + + + + CLDS I + + S L ALL++N + + LK + K+ I + D++
Sbjct: 1778 LMTLLTCSPSAQNFASKAGLIDSCVEQLKQSYSQLES-----------------VRPGKVSLHKKV------GIVWLETF---HLSMSTAATDARLSQAVHGLWPWLLLDDPTMEAALEALCVYTANCTTACSALCGSGPGVVPGVKGSSSSSNSSLMHSVMKLTS--LSSNGPIQN---LAFFLLANLVASRDCRGILQKNNFLQVFLSVPVPKAPGVKATPAGGSGSLLGLWLRLLVSLSSAEDGQQSILKVTGS-----LEMLADLATHRRHALLILHNLCFCSANKSHVLTSDKA-VNVLLSCLDSKDIEARCIGASALWALLHNNQRAKTSLKCPTVRLKIEEAHAISKKDAE 2098
BLAST of Rotatin vs. Ensembl Medaka
Match: rttn (rotatin [Source:NCBI gene;Acc:100302007]) HSP 1 Score: 58.9214 bits (141), Expect = 5.344e-8 Identity = 42/131 (32.06%), Postives = 68/131 (51.91%), Query Frame = 2
Query: 560 KEIADIEQENLLKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLK------LLLRFISEKQY-STIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQN 931
K+IA E LK SV P + LT D+++LS + +L S + +V + + DVI++DFP+ +FLQR G++K L E Y + A+ + +LC + + + DP F S K ++QN
Sbjct: 159 KKIAVNESVRCLKFSVFPWLALTNTDRHILSSNESSLGSTNPNLVRTTCELLCDVIMQDFPAEIFLQRPGIVKNLLSLLRLSSCKGEVNYLRSPALSCLRQLCVGLRRRLRFHQDPGFCSAKQ--DAVSQN 287 HSP 2 Score: 57.7658 bits (138), Expect = 1.262e-7 Identity = 33/91 (36.26%), Postives = 51/91 (56.04%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQL 321
L+ + K+ H L E+R RALKNI K++ +I++ D+ + K YLLEWFN V + VL L+ S + + +G ++FLT L
Sbjct: 3 LSSLIKKIGHSLVEIRVRALKNILCKLDHSLISVNDIVQEKMLFVYLLEWFNFPEVPMKEEVLGLLLTLSRHPSAAQMLRDVGAVDFLTSL 93
BLAST of Rotatin vs. Ensembl Medaka
Match: rttn (rotatin [Source:NCBI gene;Acc:100302007]) HSP 1 Score: 58.5362 bits (140), Expect = 6.498e-8 Identity = 40/122 (32.79%), Postives = 64/122 (52.46%), Query Frame = 2
Query: 560 KEIADIEQENLLKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLK------LLLRFISEKQY-STIAVKAISKLCYKMLQYINTCLDPNFFSTK 904
K+IA E LK SV P + LT D+++LS + +L S + +V + + DVI++DFP+ +FLQR G++K L E Y + A+ + +LC + + + DP F S K
Sbjct: 159 KKIAVNESVRCLKFSVFPWLALTNTDRHILSSNESSLGSTNPNLVRTTCELLCDVIMQDFPAEIFLQRPGIVKNLLSLLRLSSCKGEVNYLRSPALSCLRQLCVGLRRRLRFHQDPGFCSAK 280 HSP 2 Score: 58.151 bits (139), Expect = 8.059e-8 Identity = 33/91 (36.26%), Postives = 51/91 (56.04%), Query Frame = 1
Query: 49 LTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQL 321
L+ + K+ H L E+R RALKNI K++ +I++ D+ + K YLLEWFN V + VL L+ S + + +G ++FLT L
Sbjct: 3 LSSLIKKIGHSLVEIRVRALKNILCKLDHSLISVNDIVQEKMLFVYLLEWFNFPEVPMKEEVLGLLLTLSRHPSAAQMLRDVGAVDFLTSL 93
BLAST of Rotatin vs. Planmine SMEST
Match: SMESG000018486.1 (SMESG000018486.1) HSP 1 Score: 3902.06 bits (10118), Expect = 0.000e+0 Identity = 1986/1999 (99.35%), Postives = 1993/1999 (99.70%), Query Frame = 2
Query: 512 FNSPLSKNRNENSNFMKEIADIEQENLLKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFISEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQNYGEYEEDNHWNSDDLLGFHYEPNFGLPEPLPVPVFCLQVINVCLYHILEIDTDSNWAELFHLICTAIELFRESFGSDLRQPYLLELWNLKDQINDVLTLWSRLLKQYSIIEDMNYRKLSNKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSSESLIKFFLNNNEPIKYAEQCISAAENSLDCLEYVYGVRFCKEFAHFFSLISSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRXXXXXXXXXXXXXXXXXXXKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVNDNKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQXXXXXXXXXXXXRNHAFLNVDIYDIFNETSLWISTFHKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQGNTLKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLRKSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSMEIPIDVNQNDENEDISSLFIDRNSSGKYQEINGFYQKLLYLDFFRKAGLSLTNFYTLPFINCSYFKHKPLYDPEYQINFDSDFQKSVNINQILSQMRTACEINISTPLMISALVSFLCNLYYYLPDQVSSQFNELRINTLIMNMFDPHTLKVYMESFQTEHYSTTVDLVLAFTNCVKLIRLQINMNSSLKDSFLIDEKFIETIIQLLIVTKPTSDFDESHDNRLMKLDLYKYISQLRVEILYLLISLVIPTPIKNHVQLMLAHVASHVTGIFNCIKEMIEIESRKSGNVALCFVSVFLCLSSSESKFNSLIRSLESQRFSNSERVIIEDFTELLLKKRESLSRINAKSHSYTDTSINERINVHNTLKILLGTSQVVKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSMGFQIIVYRLWTVALLDSGXXXXXXXXXXXXSADTINTAKAIVICHQVHXXXXXXXXXXXXISLIENICRLTSADLTAYYLRENTVKLIFLLLSNLVWCPEGINILLRIRFLDKFEQINPNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKLMSLREIYENDSKNGLLLDIISLLNAFQDPKEFRTQ 6508
FNSPLSKNRNENSNFMKEIADIEQENLLKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFISEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQNYGEYEEDNHWNSDDLLGFHYEPNFGLPEPLPVPVFCLQVINVCLYHILEIDTDSNWAELFHLICTAIELFRESFGSDLRQPYLLELWNLKDQINDVLTLWSRLLKQ SIIEDMNYRKLSNKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQS ESLIKFFLNNNEPIKYAEQCISAAENSLDCLEYVYGV+FCKEFAHFFSLISSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRQILQLLLSNSSNIDNFLDDKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVNDNKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQSSLISIEL+LILRNHAFLNVDIYDIFNETSLWISTF KFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQGNTLKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLRKSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSMEIPIDVNQNDENEDISSLFIDRNSSGKYQEINGFYQKLLYLDFFRKAGLSLTNFYTLPFINCSYFKHKPLYDPEYQINFDSDFQKSVNINQILSQMRTACEINISTPLMIS+LVSFLCNLYYYLPDQVSSQFNELRINTLIMNMFDPHTLKVYMESFQTEHYSTTVDLVLAFTNCVKLIRLQINMNSSLKDSFLIDEKFIETIIQLLIVTKPTSD DESHDNRLMKLDLYKYISQLRVEILYLLISLVIPTPIKNHVQLMLAHVASHVTGIFNC+KEMIEIE RKSGNVALCFVSVFLCLSSSESKFNSLIRSLESQRFSNSERVIIEDFTELLLKKRESLSRINAKSHSYTDT INERINVHNTLKILLGTSQV+KNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVS+GFQIIVYRLWTVALLDSGLLHCILNCLINLSADTINTAKAIVICHQVHQQSGQPPSQGSSISLIENICRLTSADLTAY+LRENTVKLIFLLLSNLVWCPEGINILLRIRFLDKFEQINPNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKLMSLREIYENDSKNGLLLDIISLLNAFQDPKEFRTQ
Sbjct: 163 FNSPLSKNRNENSNFMKEIADIEQENLLKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIQFFTDVILEDFPSPVFLQRQGVLKLLLRFISEKQYSTIAVKAISKLCYKMLQYINTCLDPNFFSTKNTFGIINQNYGEYEEDNHWNSDDLLGFHYEPNFGLPEPLPVPVFCLQVINVCLYHILEIDTDSNWAELFHLICTAIELFRESFGSDLRQPYLLELWNLKDQINDVLTLWSRLLKQCSIIEDMNYRKLSNKRQIYLCLFAIFWNFISSIFSPEMALKYLQEHTKTELCRAQFDKCLLLGLGENSVQLITYIELFDQNFAKLNKESNRLMQSFESLIKFFLNNNEPIKYAEQCISAAENSLDCLEYVYGVQFCKEFAHFFSLISSTEIFGNREDMFIKCDEILQELMKSTNHSICKSIFTEIFDIVQNSLTIEEAAKPKSNSENKINFLLRNNIFKNLILFGLYNELKEIHETATALFCKLWEGQRIMSVIIWRQVMNITWKHMIYLQPLCDLNTRVGRQILQLLLSNSSNIDNFLDDKISNQRNFQNNIIKFLPLFYNKNYDVRKEACQYLEQFLRFDRLWNCQLNIQNMTDFLVSSVNDNKHERWFPRLLNGQSKSLDIDNFKKIFDIYLSSNIDISVRKSAAEQLEIFLQMGNMHNYFISNGGLHYVLELLKGKILDKSHLSILVPSTILILLRIVQHTNEIRKNLYDDSLLIDNLIQMALYFCYDKQCVADISSILFLVIFNSVLKQDNLSAEIKILEIIGDRILLPFRVGKYSANKTSKTEWTFSNFSKIPNQKWNYQESFERNLRIHWTLLKHGGLNNYLRYKYSFYGKKIIDSDDNYDLIDEILPCVQKLTLTESDLYVLNNSIPQFILCDNLHQIDNAINHCQVQSSLISIELILILRNHAFLNVDIYDIFNETSLWISTFQKFLLNIPSCSADFILLEFIMDVLENIIKLSMTNHMKDFIQWLVQIMTTPNNSLFYFLEKGDKLIGKTDSRRLTLAKNKIESRLPNFFLLLMRYLNLNNIQLSSKQSNTRSDIIKIHKMVFDICLNQLTQYLNYPFDNLSKLLIHIHLMSRSLELKSNFFQGNTLKVISNVINLCGDLILKFHTGREESSITFMGSGVVYYLLRILIELICNHLDFEVDRLIETTVCQTDWLRKSPWLVELIGYRSVDIRAMSATIISKMIQYEQSRSILFRHCSEILPESLWSVALTLYLDISEAPIVRSEAISILQNLLGKLPFPNSMEIPIDVNQNDENEDISSLFIDRNSSGKYQEINGFYQKLLYLDFFRKAGLSLTNFYTLPFINCSYFKHKPLYDPEYQINFDSDFQKSVNINQILSQMRTACEINISTPLMISSLVSFLCNLYYYLPDQVSSQFNELRINTLIMNMFDPHTLKVYMESFQTEHYSTTVDLVLAFTNCVKLIRLQINMNSSLKDSFLIDEKFIETIIQLLIVTKPTSDIDESHDNRLMKLDLYKYISQLRVEILYLLISLVIPTPIKNHVQLMLAHVASHVTGIFNCVKEMIEIEGRKSGNVALCFVSVFLCLSSSESKFNSLIRSLESQRFSNSERVIIEDFTELLLKKRESLSRINAKSHSYTDTRINERINVHNTLKILLGTSQVIKNYLVRVGFIESTSVEIKYLHSKLDSCAQNNVDVCIRQNKGSVVNENKISSWKQIIKEIISVLELLQNFFYEDVNAQSVAVSIGFQIIVYRLWTVALLDSGLLHCILNCLINLSADTINTAKAIVICHQVHQQSGQPPSQGSSISLIENICRLTSADLTAYHLRENTVKLIFLLLSNLVWCPEGINILLRIRFLDKFEQINPNQLQKSASGIAIQNLWLGLIISLSFTEEGQNAIMKSNGSGIMKVLNVQNNILVLRLSTLLIIRNMCTHPVIKVKLMVNGSSLIENIKHCLDSNHICIKQLSLSTLKALLYSNNKIRVYLKSANLVTKLMSLREIYENDSKNGLLLDIISLLNAFQDPKEFRTQ 2161 HSP 2 Score: 295.819 bits (756), Expect = 4.777e-80 Identity = 160/161 (99.38%), Postives = 160/161 (99.38%), Query Frame = 1
Query: 28 MSFEAKFLTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDXXXXXXXXXXXXEFLTQLKLYSNTSNFQILDQIIKNLLKVNSNDTILSENEGFSCSLLPKVNKLTKNKIDSEEKHFQQAIG 510
MSFEAKFLTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDGGGKQIIKIGGIEFLTQLKLYSNTSNFQILDQIIKNLLKVNSNDTILSENEG SCSLLPKVNKLTKNKIDSEEKHFQQAIG
Sbjct: 1 MSFEAKFLTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFIPYLLEWFNHDVVKYHDSVLKLVEMASEFDGGGKQIIKIGGIEFLTQLKLYSNTSNFQILDQIIKNLLKVNSNDTILSENEGISCSLLPKVNKLTKNKIDSEEKHFQQAIG 161 The following BLAST results are available for this feature:
BLAST of Rotatin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Rotatin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Rotatin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Rotatin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of Rotatin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Rotatin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 1
BLAST of Rotatin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 2
BLAST of Rotatin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Rotatin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of Rotatin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of Rotatin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Rotatin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of Rotatin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 3
BLAST of Rotatin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30003301 ID=SMED30003301|Name=Rotatin|organism=Schmidtea mediterranea sexual|type=transcript|length=6579bpback to top protein sequence of SMED30003301-orf-1 >SMED30003301-orf-1 ID=SMED30003301-orf-1|Name=SMED30003301-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=172bp MSFEAKFLTDFVSKLNHPLDEVRFRALKNISNKVERCIITIEDLCKHKEFback to top protein sequence of SMED30003301-orf-2 >SMED30003301-orf-2 ID=SMED30003301-orf-2|Name=SMED30003301-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1985bp MKEIADIEQENLLKLSVHPIIPLTVADKNVLSGSLGTLRSDDFRVVISGIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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