SMED30003294

Overview
NameSMED30003294
Smed IDSMED30003294
Length (bp)1253
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003294 (SMED30003294) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003294 (SMED30003294) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003294 (SMED30003294) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003294 (SMED30003294) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003294

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30003294h1SMcG0015625 dd_Smed_v4_16153_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003294h1SMcG0015625 dd_Smed_v4_16153_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30003294h1SMcG0015625 dd_Smed_v4_16153_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30003294 vs. TrEMBL
Match: A0A5K3EP67 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 5.929e-14
Identity = 44/128 (34.38%), Postives = 69/128 (53.91%), Query Frame = 2 
Query:  680 NCQCTNKNEKCLPTTIQKKLHYVVVDDKS-GQEYTYALYLSQHVGCTCKPKCLNRKCIEPF-FKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 1057
            +C C    E+CLPT ++ K+  V+V + S G   T  + L++HV C C+ +C NR+CI P          C C CP      C+ +L GR KF+  +    ++G+ + P C + P+ E  LY++ CPR
Sbjct:  444 SCTCLLPEEECLPTKVRLKVASVIVYNTSKGSTSTEVIALTEHVSCGCQRRCENRRCIPPLQLNNYTYDDCYCSCP-EGNTRCQALLDGRAKFTDKELQIPLNGSYILPPCYHGPMDEVHLYHRHCPR 570
[Close]
BLAST of SMED30003294 vs. TrEMBL
Match: A0A068WE22 (Uncharacterized protein OS=Echinococcus granulosus OX=6210 GN=EgrG_000830700 PE=4 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 1.560e-12
Identity = 46/131 (35.11%), Postives = 68/131 (51.91%), Query Frame = 2 
Query:  680 NCQCTNKNEKCLPTTIQKKLHYVVV-DDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPF----FKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 1057
            +C C    E CLPT ++ K+  VVV +   G   T  + L++H+ C C+ +C NRKCI P     +  D    C C C       CE +L GR KF+  +    ++G+ + P C + P+ E  LY+K CPR
Sbjct:  580 SCNCFLPEEVCLPTEVRLKIAPVVVYNTAKGTTTTEIVALTEHIKCRCQRRCENRKCISPLRLNIYTYDD---CYCACAESD-ERCEALLDGRAKFTDEELPIPLNGSYILPPCYHGPMDEVHLYHKHCPR 706
[Close]
BLAST of SMED30003294 vs. TrEMBL
Match: A0A4S2LMS5 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_007441 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.547e-12
Identity = 36/127 (28.35%), Postives = 69/127 (54.33%), Query Frame = 2 
Query:  680 NCQCTNKNEKCLPTTIQKKLHYVVVDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPF-FKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 1057
            +C C N +++CLPT +  K+  V    + G   T  + +++H+ C+C+P+C NR C+ P   +      C C+C  +    C+++L+G ++F   +    +SG+ + P C    + E  L+++ CPR
Sbjct:  519 SCYCLNPDQECLPTKVTTKMQPVGYPTRQGYWSTDLIVVTEHLACSCQPRCQNRMCVAPLKLRMSTTSHCSCVCELNDMR-CQKLLEGEEQFQPDELPVPLSGSFILPPCRFGSMDEYHLFHRRCPR 644
[Close]
BLAST of SMED30003294 vs. TrEMBL
Match: A0A068Y851 (Uncharacterized protein OS=Echinococcus multilocularis OX=6211 GN=EmuJ_000830700 PE=4 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 2.766e-12
Identity = 45/131 (34.35%), Postives = 68/131 (51.91%), Query Frame = 2 
Query:  680 NCQCTNKNEKCLPTTIQKKLHYVVV-DDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPF----FKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 1057
            +C C    E CLPT ++ K+  VVV +   G   T  + L++H+ C C+ +C NRKCI P     +  D    C C C       CE +L GR +F+  +    ++G+ + P C + P+ E  LY+K CPR
Sbjct:  580 SCNCFLPEEVCLPTEVRLKIAPVVVYNTAKGTTTTEIVALTEHIKCRCQRRCENRKCISPLQLNIYTYDD---CYCACAESD-ERCEALLDGRARFTDEELPIPLNGSYILPPCYHGPMDEVHLYHKHCPR 706
[Close]
BLAST of SMED30003294 vs. TrEMBL
Match: A0A564YEW7 (Uncharacterized protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS5689 PE=4 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 3.089e-12
Identity = 43/128 (33.59%), Postives = 70/128 (54.69%), Query Frame = 2 
Query:  680 NCQCTNKNEKCLPTTIQKKLHYVVV-DDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPF-FKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 1057
            +C C    E+CLPT ++ K   VVV +   G   T  + L++H+GC CK +C NR+C+ P    +     C C C +     CE +L+G++KF++ +    ++G+ + P C +  L E  LY + CPR
Sbjct:  487 SCTCALPEEECLPTEVRLKTSPVVVYNTAEGTTTTEVIALTEHMGCQCKRRCDNRQCVPPLKLNKYTYDDCYCYCEKGDA-RCEALLEGQEKFTEKELPTPLNGSFIMPPCYHGQLDEVHLYFRHCPR 613
[Close]
BLAST of SMED30003294 vs. Planmine SMEST
Match: SMESG000042814.1 (SMESG000042814.1)

HSP 1 Score: 643.269 bits (1658), Expect = 0.000e+0
Identity = 316/318 (99.37%), Postives = 316/318 (99.37%), Query Frame = 2 
Query:  104 FFVIWIIFFTAIYQSLMVQTYPNSIKKSIFQRKFQIKLQGNSSNNIKLESFTQYLINLYNLEKDNLTILSVIERWRKFVEDFDTLLLSKMNKTISKPIIPETKKLIRKRHHGNAIYKNSIVLKRQLSKLRFDKNEKQKALEEEYELKQSNFGVCKPQNQTFCMKRFFSLKPGSEYIVLFRPAVLPVQRCPKDNCQCTNKNEKCLPTTIQKKLHYVVVDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPFFKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 1057
            F  IWIIFFTAIYQSLMVQTYPNSIKKSIFQRKFQIKLQGNSSNNIKLESFTQYLINLYNLEKDNLTILSVIERWRKFVEDFDTLLLSKMNKTISKPIIPETKKLIRKRHHGNAIYKNSIVLKRQLSKLRFDKNEKQKALEEEYELKQSNFGVCKPQNQTFCMKRFFSLKPGSEYIVLFRPAVLPVQRCPKDNCQCTNKNEKCLPTTIQKKLHYVVVDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPFFKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR
Sbjct:    9 FCQIWIIFFTAIYQSLMVQTYPNSIKKSIFQRKFQIKLQGNSSNNIKLESFTQYLINLYNLEKDNLTILSVIERWRKFVEDFDTLLLSKMNKTISKPIIPETKKLIRKRHHGNAIYKNSIVLKRQLSKLRFDKNEKQKALEEEYELKQSNFGVCKPQNQTFCMKRFFSLKPGSEYIVLFRPAVLPVQRCPKDNCQCTNKNEKCLPTTIQKKLHYVVVDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPFFKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 326
[Close]
BLAST of SMED30003294 vs. Planmine SMEST
Match: SMESG000042814.1 (SMESG000042814.1)

HSP 1 Score: 642.884 bits (1657), Expect = 0.000e+0
Identity = 316/318 (99.37%), Postives = 316/318 (99.37%), Query Frame = 2 
Query:  104 FFVIWIIFFTAIYQSLMVQTYPNSIKKSIFQRKFQIKLQGNSSNNIKLESFTQYLINLYNLEKDNLTILSVIERWRKFVEDFDTLLLSKMNKTISKPIIPETKKLIRKRHHGNAIYKNSIVLKRQLSKLRFDKNEKQKALEEEYELKQSNFGVCKPQNQTFCMKRFFSLKPGSEYIVLFRPAVLPVQRCPKDNCQCTNKNEKCLPTTIQKKLHYVVVDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPFFKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 1057
            F  IWIIFFTAIYQSLMVQTYPNSIKKSIFQRKFQIKLQGNSSNNIKLESFTQYLINLYNLEKDNLTILSVIERWRKFVEDFDTLLLSKMNKTISKPIIPETKKLIRKRHHGNAIYKNSIVLKRQLSKLRFDKNEKQKALEEEYELKQSNFGVCKPQNQTFCMKRFFSLKPGSEYIVLFRPAVLPVQRCPKDNCQCTNKNEKCLPTTIQKKLHYVVVDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPFFKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR
Sbjct:    6 FCQIWIIFFTAIYQSLMVQTYPNSIKKSIFQRKFQIKLQGNSSNNIKLESFTQYLINLYNLEKDNLTILSVIERWRKFVEDFDTLLLSKMNKTISKPIIPETKKLIRKRHHGNAIYKNSIVLKRQLSKLRFDKNEKQKALEEEYELKQSNFGVCKPQNQTFCMKRFFSLKPGSEYIVLFRPAVLPVQRCPKDNCQCTNKNEKCLPTTIQKKLHYVVVDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPFFKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 323
[Close]
BLAST of SMED30003294 vs. Planmine SMEST
Match: SMESG000042814.1 (SMESG000042814.1)

HSP 1 Score: 614.764 bits (1584), Expect = 0.000e+0
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 2 
Query:  152 MVQTYPNSIKKSIFQRKFQIKLQGNSSNNIKLESFTQYLINLYNLEKDNLTILSVIERWRKFVEDFDTLLLSKMNKTISKPIIPETKKLIRKRHHGNAIYKNSIVLKRQLSKLRFDKNEKQKALEEEYELKQSNFGVCKPQNQTFCMKRFFSLKPGSEYIVLFRPAVLPVQRCPKDNCQCTNKNEKCLPTTIQKKLHYVVVDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPFFKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 1057
            MVQTYPNSIKKSIFQRKFQIKLQGNSSNNIKLESFTQYLINLYNLEKDNLTILSVIERWRKFVEDFDTLLLSKMNKTISKPIIPETKKLIRKRHHGNAIYKNSIVLKRQLSKLRFDKNEKQKALEEEYELKQSNFGVCKPQNQTFCMKRFFSLKPGSEYIVLFRPAVLPVQRCPKDNCQCTNKNEKCLPTTIQKKLHYVVVDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPFFKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR
Sbjct:    1 MVQTYPNSIKKSIFQRKFQIKLQGNSSNNIKLESFTQYLINLYNLEKDNLTILSVIERWRKFVEDFDTLLLSKMNKTISKPIIPETKKLIRKRHHGNAIYKNSIVLKRQLSKLRFDKNEKQKALEEEYELKQSNFGVCKPQNQTFCMKRFFSLKPGSEYIVLFRPAVLPVQRCPKDNCQCTNKNEKCLPTTIQKKLHYVVVDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPFFKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 302
[Close]
BLAST of SMED30003294 vs. Planmine SMEST
Match: SMESG000020729.1 (SMESG000020729.1)

HSP 1 Score: 100.138 bits (248), Expect = 4.034e-25
Identity = 55/135 (40.74%), Postives = 81/135 (60.00%), Query Frame = 2 
Query:  671 PKDNCQCTNKNEKCLPTTIQKKLHYVVVD---DKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPF---FKRDKNGSCKCLCPRHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMCPR 1057
            PKD C C+  +EKCLP  ++ +  Y++ +   + +  E T  L L  H  C C+ +CLNRKCI  F   +K   + SCKC   +  +  C+++L+G   F+++DY CI+SG CL PDCL   LSEE LY + CP+
Sbjct:    2 PKD-CTCSQTHEKCLPRLVRNRTEYIIANNIVETNFVEETLTLNLVDHENCGCRSRCLNRKCISNFEFDYKSQADCSCKC---KDNQKLCQKILKGYHNFTEADYLCILSGECLAPDCLGGQLSEELLYLRKCPQ 132
The following BLAST results are available for this feature:
BLAST of SMED30003294 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5K3EP675.929e-1434.38Uncharacterized protein OS=Mesocestoides corti OX=... [more]
A0A068WE221.560e-1235.11Uncharacterized protein OS=Echinococcus granulosus... [more]
A0A4S2LMS52.547e-1228.35Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A068Y8512.766e-1234.35Uncharacterized protein OS=Echinococcus multilocul... [more]
A0A564YEW73.089e-1233.59Uncharacterized protein OS=Hymenolepis diminuta OX... [more]
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BLAST of SMED30003294 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003294 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000042814.10.000e+099.37SMESG000042814.1[more]
SMESG000042814.10.000e+099.37SMESG000042814.1[more]
SMESG000042814.10.000e+0100.00SMESG000042814.1[more]
SMESG000020729.14.034e-2540.74SMESG000020729.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003294 ID=SMED30003294|Name=SMED30003294|organism=Schmidtea mediterranea sexual|type=transcript|length=1253bp
GTCGACAATGCTCACGAATTCGAATACAGAGATGACAGGTTTCATTTCAA
AATCAAAATTATCAGAATTGTCATTGTAACAATGTGTTCAATGATTCGGT
TACTTTTTTGTCATTTGGATCATATTTTTCACCGCCATTTATCAATCTCT
GATGGTGCAAACATATCCGAATTCAATCAAAAAATCCATATTTCAAAGAA
AGTTTCAAATAAAATTACAAGGAAATTCTTCCAACAACATCAAACTAGAA
TCATTTACTCAGTATCTCATCAATCTTTATAACTTAGAAAAAGATAATTT
AACAATTTTATCTGTTATTGAAAGATGGAGGAAATTCGTGGAAGACTTTG
ATACTTTATTATTATCAAAAATGAATAAAACAATTTCAAAACCAATAATT
CCAGAAACTAAGAAATTAATCAGGAAAAGGCATCACGGGAATGCCATCTA
TAAAAACAGTATTGTGTTAAAACGACAGTTAAGTAAACTCAGATTTGATA
AAAATGAGAAACAAAAAGCATTGGAAGAAGAATACGAGTTAAAACAATCA
AATTTCGGAGTTTGCAAGCCACAGAATCAAACGTTTTGTATGAAACGGTT
TTTCAGTTTAAAACCGGGTAGTGAATATATTGTTTTATTTCGCCCGGCTG
TTCTACCCGTTCAGAGATGTCCAAAAGACAATTGCCAGTGCACAAACAAA
AACGAAAAGTGTCTCCCAACAACAATTCAGAAGAAACTGCATTATGTGGT
TGTAGACGATAAAAGTGGTCAGGAATATACTTACGCTTTATATTTATCCC
AGCATGTCGGTTGTACTTGTAAACCAAAATGCTTGAATAGAAAATGCATT
GAACCGTTTTTCAAGCGAGACAAAAACGGATCGTGTAAATGTCTCTGCCC
AAGGCATAAACGGAATGAATGCGAACAAGTTCTACAGGGTCGAAAGAAAT
TCTCAAAATCAGACTACAACTGCATTGTTTCTGGGAATTGTCTAACACCA
GATTGTTTGAATCAGCCTCTTTCAGAAGAAAGTCTTTACAACAAAATGTG
TCCTAGGTAATATTGGATAGAGAGTCACGTAAAAATCCTCACTAGTCATC
AATAGTAATATTACAATTCAGTCGGAATTCGAATCATTTACAATAAATCT
CAATATCAAATTCCCATTCCTGACAAATCAGGTTATCTACAGCTTATTTT
TATTTGAGTTTGATTTATTAAAAGAATTTAAATGGAATTTAATAAAAATG
GAT
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protein sequence of SMED30003294-orf-1

>SMED30003294-orf-1 ID=SMED30003294-orf-1|Name=SMED30003294-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=303bp
MVQTYPNSIKKSIFQRKFQIKLQGNSSNNIKLESFTQYLINLYNLEKDNL
TILSVIERWRKFVEDFDTLLLSKMNKTISKPIIPETKKLIRKRHHGNAIY
KNSIVLKRQLSKLRFDKNEKQKALEEEYELKQSNFGVCKPQNQTFCMKRF
FSLKPGSEYIVLFRPAVLPVQRCPKDNCQCTNKNEKCLPTTIQKKLHYVV
VDDKSGQEYTYALYLSQHVGCTCKPKCLNRKCIEPFFKRDKNGSCKCLCP
RHKRNECEQVLQGRKKFSKSDYNCIVSGNCLTPDCLNQPLSEESLYNKMC
PR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR029034Cystine-knot_cytokine
Vocabulary: molecular function
TermDefinition
GO:0008083growth factor activity
Vocabulary: cellular component
TermDefinition
GO:0016020membrane