SMED30003270

Overview
NameSMED30003270
Smed IDSMED30003270
Length (bp)1428
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003270 (SMED30003270) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003270 (SMED30003270) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003270 (SMED30003270) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003270 (SMED30003270) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003270

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30003270h1SMcG0015304 Contig49605uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003270h1SMcG0015304 Contig49605newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30003270h1SMcG0015304 dd_Smed_v4_3513_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30003270h1SMcG0015304 dd_Smed_v4_3513_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003270h1SMcG0015304 dd_Smed_v4_3513_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30003270h1SMcG0015304 dd_Smed_v4_3513_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30003270h1SMcG0015304 dd_Smed_v4_3513_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003270h1SMcG0015304 dd_Smed_v4_3513_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30003270h1SMcG0015304 dd_Smed_v4_3513_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
enteric muscle cellSMED30003270h1SMcG0015304 dd_Smed_v4_3513_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003270 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005447:14564..15991 +7122
Homology
BLAST of SMED30003270 vs. Ensembl Human
Match: IGFALS (insulin like growth factor binding protein acid labile subunit [Source:HGNC Symbol;Acc:HGNC:5468])

HSP 1 Score: 82.8037 bits (203), Expect = 3.908e-16
Identity = 58/186 (31.18%), Postives = 99/186 (53.23%), Query Frame = 1
Query:  355 PQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            P  F+ +S L  L +Q   + ++E Q L    +L  LH+   ++  L+ G F+  P    + LS+NR+S + D  F+    + +L+LG N L V+    F    SL+ L L+ NR+  ++   F  L +++ L+L+ N L  IK  +   L  LQ L+L +N I  +   AF+ LK+L++++L  N
Sbjct:  129 PAAFQNLSSLGFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALGTFAHTPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGLGSLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHN 314          

HSP 2 Score: 52.7582 bits (125), Expect = 1.178e-6
Identity = 44/149 (29.53%), Postives = 74/149 (49.66%), Query Frame = 1
Query:  469 SETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            S   I  L    F D    E ++L  NRI  +A+++F+   ++  L L +N+L  ++   F    ++  ++LS N +  + +  F+ L K+  L+L  + L RI+P    GL  L+ LFL  N +  IEE +   L  L  ++L  N L
Sbjct:  336 SHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFTGLSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQL 484          

HSP 3 Score: 52.373 bits (124), Expect = 1.625e-6
Identity = 54/221 (24.43%), Postives = 94/221 (42.53%), Query Frame = 1
Query:  334 SSLIVLMPQMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNR------------------------LTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            +SL VL    F+ +  L  LV+    +  ++         L+ L +S   +  +   +F   P  +++ L  N I+ +A   F     +  LDL +NR                        +  +R   F + H L+ L L  NRI ++ + SF+ L +++VL L+ N L  +K     GL ++  + LS N +  + E  F  L  L  ++LE + L R
Sbjct:  218 NSLAVLPDAAFRGLGSLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGR 438          
BLAST of SMED30003270 vs. Ensembl Human
Match: IGFALS (insulin like growth factor binding protein acid labile subunit [Source:HGNC Symbol;Acc:HGNC:5468])

HSP 1 Score: 82.4185 bits (202), Expect = 4.530e-16
Identity = 58/186 (31.18%), Postives = 99/186 (53.23%), Query Frame = 1
Query:  355 PQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            P  F+ +S L  L +Q   + ++E Q L    +L  LH+   ++  L+ G F+  P    + LS+NR+S + D  F+    + +L+LG N L V+    F    SL+ L L+ NR+  ++   F  L +++ L+L+ N L  IK  +   L  LQ L+L +N I  +   AF+ LK+L++++L  N
Sbjct:   91 PAAFQNLSSLGFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALGTFAHTPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGLGSLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHN 276          

HSP 2 Score: 52.7582 bits (125), Expect = 1.229e-6
Identity = 44/149 (29.53%), Postives = 74/149 (49.66%), Query Frame = 1
Query:  469 SETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            S   I  L    F D    E ++L  NRI  +A+++F+   ++  L L +N+L  ++   F    ++  ++LS N +  + +  F+ L K+  L+L  + L RI+P    GL  L+ LFL  N +  IEE +   L  L  ++L  N L
Sbjct:  298 SHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFTGLSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQL 446          

HSP 3 Score: 51.9878 bits (123), Expect = 1.881e-6
Identity = 54/221 (24.43%), Postives = 94/221 (42.53%), Query Frame = 1
Query:  334 SSLIVLMPQMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNR------------------------LTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            +SL VL    F+ +  L  LV+    +  ++         L+ L +S   +  +   +F   P  +++ L  N I+ +A   F     +  LDL +NR                        +  +R   F + H L+ L L  NRI ++ + SF+ L +++VL L+ N L  +K     GL ++  + LS N +  + E  F  L  L  ++LE + L R
Sbjct:  180 NSLAVLPDAAFRGLGSLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGR 400          
BLAST of SMED30003270 vs. Ensembl Human
Match: LRIG3 (leucine rich repeats and immunoglobulin like domains 3 [Source:HGNC Symbol;Acc:HGNC:30991])

HSP 1 Score: 67.781 bits (164), Expect = 2.419e-11
Identity = 59/194 (30.41%), Postives = 101/194 (52.06%), Query Frame = 1
Query:  349 LMPQMFKLVSRLETLVV-QAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDF--SFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            LM   F  +S +E L +    +  I   +L   + L+ LH+S+  I  +S   +  C     + L+ N +S + D +F   + +  L +GNNR++ I    F    SL+ LDL  N I+  IED   +F  L K++ L L  N +  I  +   GL++L++L LS N+I +++ +AF  +K L+ ++L  + LL
Sbjct:  254 LMDGAFWGLSNMEILQLDHNNLTEITKGWLYGLLMLQELHLSQNAINRISPDAWEFCQKLSELDLTFNHLSRLDDSSFLGLSLLNTLHIGNNRVSYIADCAFRGLSSLKTLDLKNNEISWTIEDMNGAFSGLDKLRRLILQGNRIRSITKKAFTGLDALEHLDLSDNAIMSLQGNAFSQMKKLQQLHLNTSSLL 447          
BLAST of SMED30003270 vs. Ensembl Human
Match: LRIG3 (leucine rich repeats and immunoglobulin like domains 3 [Source:HGNC Symbol;Acc:HGNC:30991])

HSP 1 Score: 67.781 bits (164), Expect = 2.832e-11
Identity = 59/194 (30.41%), Postives = 101/194 (52.06%), Query Frame = 1
Query:  349 LMPQMFKLVSRLETLVV-QAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDF--SFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            LM   F  +S +E L +    +  I   +L   + L+ LH+S+  I  +S   +  C     + L+ N +S + D +F   + +  L +GNNR++ I    F    SL+ LDL  N I+  IED   +F  L K++ L L  N +  I  +   GL++L++L LS N+I +++ +AF  +K L+ ++L  + LL
Sbjct:  194 LMDGAFWGLSNMEILQLDHNNLTEITKGWLYGLLMLQELHLSQNAINRISPDAWEFCQKLSELDLTFNHLSRLDDSSFLGLSLLNTLHIGNNRVSYIADCAFRGLSSLKTLDLKNNEISWTIEDMNGAFSGLDKLRRLILQGNRIRSITKKAFTGLDALEHLDLSDNAIMSLQGNAFSQMKKLQQLHLNTSSLL 387          
BLAST of SMED30003270 vs. Ensembl Human
Match: LRIG1 (leucine rich repeats and immunoglobulin like domains 1 [Source:HGNC Symbol;Acc:HGNC:17360])

HSP 1 Score: 67.3958 bits (163), Expect = 3.352e-11
Identity = 63/218 (28.90%), Postives = 100/218 (45.87%), Query Frame = 1
Query:  343 IVLMPQMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCN------------------------RVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            +  +P +    S + +L +Q   I+++E   LK ++SL++L +S   I  +    F   P  + + L+ NRI  +    FD  +                        R+  LDL  NR+ +I  L F   +SL+ L L RN I+++ D +F  L KM VL+L  N L+ +    L+GL +L  L LS NSI  I    +   + L  + L  N L R
Sbjct:  104 LTAVPSLGAASSHVVSLFLQHNKIRSVEGSQLKAYLSLEVLDLSLNNITEVRNTCFPHGPPIKELNLAGNRIGTLELGAFDGLSRSLLTLRLSKNRITQLPVRAFKLPRLTQLDLNRNRIRLIEGLTFQGLNSLEVLKLQRNNISKLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSIARIHRKGWSFCQKLHELVLSFNNLTR 321          
BLAST of SMED30003270 vs. Ensembl Celegans
Match: sma-10 (Leucine-rich repeats and immunoglobulin-like domains protein sma-10 [Source:UniProtKB/Swiss-Prot;Acc:Q965M2])

HSP 1 Score: 66.6254 bits (161), Expect = 1.991e-11
Identity = 46/167 (27.54%), Postives = 86/167 (51.50%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            I T+ ++ L    +++ + +S   I +L +   S     E++ L+SN I+ I    F S N +V L L  N +T + +  F+    L++LDL+RN I  +   +F  L  ++ ++L  N + R+   + +  E L++L LS N ++ + E     L SL+ ++L  N
Sbjct:  135 ISTVTSEELSYLAAVRSVDLSRNLISYLPKPTTSAKVNIEKLDLASNSITDIGTDHFSSFNTLVTLKLARNHITTLNQFSFSRLRKLESLDLTRNMIREVRFLAFNQLPSLQNVSLARNDVYRLDDGMFYACEGLKHLNLSTNRVQAVTEGWMFGLTSLEVLDLSYN 301          
BLAST of SMED30003270 vs. Ensembl Celegans
Match: sma-10 (Leucine-rich repeats and immunoglobulin-like domains protein sma-10 [Source:UniProtKB/Swiss-Prot;Acc:Q965M2])

HSP 1 Score: 66.6254 bits (161), Expect = 1.991e-11
Identity = 46/167 (27.54%), Postives = 86/167 (51.50%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            I T+ ++ L    +++ + +S   I +L +   S     E++ L+SN I+ I    F S N +V L L  N +T + +  F+    L++LDL+RN I  +   +F  L  ++ ++L  N + R+   + +  E L++L LS N ++ + E     L SL+ ++L  N
Sbjct:  135 ISTVTSEELSYLAAVRSVDLSRNLISYLPKPTTSAKVNIEKLDLASNSITDIGTDHFSSFNTLVTLKLARNHITTLNQFSFSRLRKLESLDLTRNMIREVRFLAFNQLPSLQNVSLARNDVYRLDDGMFYACEGLKHLNLSTNRVQAVTEGWMFGLTSLEVLDLSYN 301          
BLAST of SMED30003270 vs. Ensembl Celegans
Match: sma-10 (Leucine-rich repeats and immunoglobulin-like domains protein sma-10 [Source:UniProtKB/Swiss-Prot;Acc:Q965M2])

HSP 1 Score: 66.6254 bits (161), Expect = 2.194e-11
Identity = 46/167 (27.54%), Postives = 86/167 (51.50%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            I T+ ++ L    +++ + +S   I +L +   S     E++ L+SN I+ I    F S N +V L L  N +T + +  F+    L++LDL+RN I  +   +F  L  ++ ++L  N + R+   + +  E L++L LS N ++ + E     L SL+ ++L  N
Sbjct:  135 ISTVTSEELSYLAAVRSVDLSRNLISYLPKPTTSAKVNIEKLDLASNSITDIGTDHFSSFNTLVTLKLARNHITTLNQFSFSRLRKLESLDLTRNMIREVRFLAFNQLPSLQNVSLARNDVYRLDDGMFYACEGLKHLNLSTNRVQAVTEGWMFGLTSLEVLDLSYN 301          
BLAST of SMED30003270 vs. Ensembl Celegans
Match: sma-10 (Leucine-rich repeats and immunoglobulin-like domains protein sma-10 [Source:UniProtKB/Swiss-Prot;Acc:Q965M2])

HSP 1 Score: 66.6254 bits (161), Expect = 2.194e-11
Identity = 46/167 (27.54%), Postives = 86/167 (51.50%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            I T+ ++ L    +++ + +S   I +L +   S     E++ L+SN I+ I    F S N +V L L  N +T + +  F+    L++LDL+RN I  +   +F  L  ++ ++L  N + R+   + +  E L++L LS N ++ + E     L SL+ ++L  N
Sbjct:  135 ISTVTSEELSYLAAVRSVDLSRNLISYLPKPTTSAKVNIEKLDLASNSITDIGTDHFSSFNTLVTLKLARNHITTLNQFSFSRLRKLESLDLTRNMIREVRFLAFNQLPSLQNVSLARNDVYRLDDGMFYACEGLKHLNLSTNRVQAVTEGWMFGLTSLEVLDLSYN 301          
BLAST of SMED30003270 vs. Ensembl Celegans
Match: lron-2 (ELRR (Extracellular Leucine-Rich Repeat) ONly [Source:UniProtKB/TrEMBL;Acc:Q23580])

HSP 1 Score: 63.5438 bits (153), Expect = 1.500e-10
Identity = 44/160 (27.50%), Postives = 81/160 (50.62%), Query Frame = 1
Query:  232 VEWPILLMTVKKLRFNADLLAACQRISGL-----LITTESSLIVLMPQMFKLVSRLETLVV-QAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRN 693
            +++P+L  +   L FN   +   Q   G+     L  T +      P+MF+ +++L+TL + + PI  I N    +  +L  L +SE  ++ + RG F   P  + I L+ NR+S +    F+  N + ++DL  NR+  ++ L F    ++ +LD+S N
Sbjct:  231 LDFPVL--SSLSLEFNGITVIPPQAFQGVPNLQFLYMTGNKFPSWAPEMFRYITQLKTLGIGETPISVIPNNAFMHIPNLIRLEMSEAAVDTIERGAFQRTPQIQAIVLNKNRLSQVRADFFEGLNDLYSIDLQGNRIDNVQPLGFANLPAISHLDISYN 388          
BLAST of SMED30003270 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330733)

HSP 1 Score: 70.0922 bits (170), Expect = 2.842e-12
Identity = 55/213 (25.82%), Postives = 101/213 (47.42%), Query Frame = 1
Query:  292 AACQRI---SGLLITTESSLIVLMPQMFKLVSRLETLVVQAPIQTI--ENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            A C R+   +GL +      +  +P+  K+ + +E L +Q    T+  E  F +    L++L +++ +I  + R  F D    ER+RL++NR+  I +    S   ++ LD+ NN +T + R +F    SL++L L  N+IT +++ +FK                        GL  L+ L L+ N++ ++  + F  L  L+ + L  NP 
Sbjct:   71 ARCPRVCSCTGLNVDCSHRGLTSVPR--KISADVERLELQGNNLTVIYETDF-QRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFK------------------------GLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPF 256          
BLAST of SMED30003270 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330731)

HSP 1 Score: 70.0922 bits (170), Expect = 2.842e-12
Identity = 55/213 (25.82%), Postives = 101/213 (47.42%), Query Frame = 1
Query:  292 AACQRI---SGLLITTESSLIVLMPQMFKLVSRLETLVVQAPIQTI--ENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            A C R+   +GL +      +  +P+  K+ + +E L +Q    T+  E  F +    L++L +++ +I  + R  F D    ER+RL++NR+  I +    S   ++ LD+ NN +T + R +F    SL++L L  N+IT +++ +FK                        GL  L+ L L+ N++ ++  + F  L  L+ + L  NP 
Sbjct:   71 ARCPRVCSCTGLNVDCSHRGLTSVPR--KISADVERLELQGNNLTVIYETDF-QRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFK------------------------GLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPF 256          
BLAST of SMED30003270 vs. Ensembl Fly
Match: sli (gene:FBgn0264089 transcript:FBtr0330734)

HSP 1 Score: 70.0922 bits (170), Expect = 3.102e-12
Identity = 55/213 (25.82%), Postives = 101/213 (47.42%), Query Frame = 1
Query:  292 AACQRI---SGLLITTESSLIVLMPQMFKLVSRLETLVVQAPIQTI--ENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            A C R+   +GL +      +  +P+  K+ + +E L +Q    T+  E  F +    L++L +++ +I  + R  F D    ER+RL++NR+  I +    S   ++ LD+ NN +T + R +F    SL++L L  N+IT +++ +FK                        GL  L+ L L+ N++ ++  + F  L  L+ + L  NP 
Sbjct:   71 ARCPRVCSCTGLNVDCSHRGLTSVPR--KISADVERLELQGNNLTVIYETDFQR-LTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFK------------------------GLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPF 256          
BLAST of SMED30003270 vs. Ensembl Fly
Match: CG7896 (gene:FBgn0039728 transcript:FBtr0085545)

HSP 1 Score: 66.6254 bits (161), Expect = 3.025e-11
Identity = 52/193 (26.94%), Postives = 97/193 (50.26%), Query Frame = 1
Query:  340 LIVLMPQMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            L  L   +FK + RL+ + +    I+ I    L     LK +++++ +++ L  G F +      I LS+NRI  I    F +  ++  LDL  N+L+  +   FN G  ++ LD+S N+++ +   SF+   +++ +    N       E++  L+ L+++ LS N ++TIEE  F  L  L+ + +  N L
Sbjct:  534 LTPLTGALFKPLDRLQVIDLSGCNIRQISGDLLAGLQDLKHIYLNDNQLQELQDGSFVNLWNISSIDLSNNRIGSIRSGAFVNVMKLQKLDLHGNQLSAFKGEYFNTGTGIEELDISDNQLSYLFPSSFRIHPRLREIRAANNKFSFFPAELISTLQYLEHIDLSHNQLKTIEELDFARLPRLRVLLVANNQL 726          

HSP 2 Score: 50.8322 bits (120), Expect = 2.443e-6
Identity = 43/170 (25.29%), Postives = 78/170 (45.88%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            I++I++   K    ++ + ++  RI HL+  +F      +++ LS N           +   + +L+L +N L  +         SL++LD+SRN IT I   +F+                         + +L+YL LS NS+ TIE+DA   L SL+ + ++ N +L
Sbjct:  244 IRSIDSLAFKGLQKIREIKLAGNRISHLNSDVFEKLQSLQKLDLSENFFGQFPTVALAAVPGLKHLNLSSNMLQQLDYTHMQVVRSLESLDISRNTITTITPGTFRE------------------------MGALKYLDLSLNSLRTIEDDALEGLDSLQTLIIKDNNIL 389          
BLAST of SMED30003270 vs. Ensembl Fly
Match: CG32055 (gene:FBgn0052055 transcript:FBtr0076304)

HSP 1 Score: 60.8474 bits (146), Expect = 1.452e-9
Identity = 62/197 (31.47%), Postives = 94/197 (47.72%), Query Frame = 1
Query:  328 TESSLIVLMPQMFKLVSRLETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIF---NEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            +E+ + V+ P  F+ +  L  L +    Q  EN  L    +L+ L IS   +           P  E + L SN +  +    F +   +  L++ NN L  I+R +F    E   L+ LD S N +  ++D  F  LKK++ LNL  N + RI P    GL SLQ L L  N I  + +D F NL +L+ ++L KN
Sbjct:  154 SENLIAVIEPNAFRHMKNLRFLDLTTNYQ--ENITLGENANLRFLSISNNNLRDFQWCHLRVLPKLEELHLHSNWLEHLDMGIFYALPNLRVLNVSNNNLFEIKRTLFMAPGEIAPLELLDYSSNIVKVLDDSVFCRLKKLRTLNLWLNQINRIHPRAFLGLSSLQTLHLQGNKISILPDDVFANLTALEKLDLSKN 348          
BLAST of SMED30003270 vs. Ensembl Zebrafish
Match: lrig1 (leucine-rich repeats and immunoglobulin-like domains 1 [Source:ZFIN;Acc:ZDB-GENE-031113-23])

HSP 1 Score: 67.781 bits (164), Expect = 1.862e-11
Identity = 56/160 (35.00%), Postives = 90/160 (56.25%), Query Frame = 1
Query:  445 VSLKILHISETRIEHLSRGMFSDCPITERI-RLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            + +K LH+S  +I HL  G F +   + +I RLS NR++ +  K  +   ++  L+L  NRL +I  L F    SL+ L L RN I+++ D +F  L +M+VL+L+ N L  +    L+GLESL  L+L+ NSI     + +   + L+ +NL  N L +
Sbjct:  130 LQIKDLHLSSNKIVHLEFGAFKNLAGSLQILRLSRNRLTHLPVKGLE-LPKLTQLELSRNRLRLIEGLTFQGLSSLEVLKLQRNNISKLTDGAFWGLARMRVLHLDYNSLREVNSGSLYGLESLLQLYLANNSISNFNPEGWGFCERLRELNLSYNNLTK 288          

HSP 2 Score: 57.3806 bits (137), Expect = 2.681e-8
Identity = 58/218 (26.61%), Postives = 109/218 (50.00%), Query Frame = 1
Query:  307 ISGLLITTESSLIVLMPQ----------MFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFS--FXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            I GL     SSL VL  Q           F  ++R+  L +    ++ + +  L    SL  L+++   I + +   +  C     + LS N ++ +++ +F     +++L LG+N ++ I    F    SL+ L+L  N I+  IED S  F  L+ +  L L  N +  +  +   GLE+L++L L +N+I +I+ +AF  +K+L+Y++++ +  L
Sbjct:  193 IEGLTFQGLSSLEVLKLQRNNISKLTDGAFWGLARMRVLHLDYNSLREVNSGSLYGLESLLQLYLANNSISNFNPEGWGFCERLRELNLSYNNLTKLSEGSFAKLVNLISLRLGHNSISHITEGAFRGLSSLRTLELDHNDISGTIEDTSGAFTGLENLNKLTLFGNKIKSVAKKAFSGLEALEHLNLGENAIRSIQPEAFSKMKNLRYLHIQSDSFL 410          
BLAST of SMED30003270 vs. Ensembl Zebrafish
Match: lrig1 (leucine-rich repeats and immunoglobulin-like domains 1 [Source:ZFIN;Acc:ZDB-GENE-031113-23])

HSP 1 Score: 67.781 bits (164), Expect = 1.943e-11
Identity = 56/160 (35.00%), Postives = 90/160 (56.25%), Query Frame = 1
Query:  445 VSLKILHISETRIEHLSRGMFSDCPITERI-RLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            + +K LH+S  +I HL  G F +   + +I RLS NR++ +  K  +   ++  L+L  NRL +I  L F    SL+ L L RN I+++ D +F  L +M+VL+L+ N L  +    L+GLESL  L+L+ NSI     + +   + L+ +NL  N L +
Sbjct:  154 LQIKDLHLSSNKIVHLEFGAFKNLAGSLQILRLSRNRLTHLPVKGLE-LPKLTQLELSRNRLRLIEGLTFQGLSSLEVLKLQRNNISKLTDGAFWGLARMRVLHLDYNSLREVNSGSLYGLESLLQLYLANNSISNFNPEGWGFCERLRELNLSYNNLTK 312          

HSP 2 Score: 57.3806 bits (137), Expect = 2.891e-8
Identity = 58/218 (26.61%), Postives = 109/218 (50.00%), Query Frame = 1
Query:  307 ISGLLITTESSLIVLMPQ----------MFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFS--FXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            I GL     SSL VL  Q           F  ++R+  L +    ++ + +  L    SL  L+++   I + +   +  C     + LS N ++ +++ +F     +++L LG+N ++ I    F    SL+ L+L  N I+  IED S  F  L+ +  L L  N +  +  +   GLE+L++L L +N+I +I+ +AF  +K+L+Y++++ +  L
Sbjct:  217 IEGLTFQGLSSLEVLKLQRNNISKLTDGAFWGLARMRVLHLDYNSLREVNSGSLYGLESLLQLYLANNSISNFNPEGWGFCERLRELNLSYNNLTKLSEGSFAKLVNLISLRLGHNSISHITEGAFRGLSSLRTLELDHNDISGTIEDTSGAFTGLENLNKLTLFGNKIKSVAKKAFSGLEALEHLNLGENAIRSIQPEAFSKMKNLRYLHIQSDSFL 434          
BLAST of SMED30003270 vs. Ensembl Zebrafish
Match: lrig1 (leucine-rich repeats and immunoglobulin-like domains 1 [Source:ZFIN;Acc:ZDB-GENE-031113-23])

HSP 1 Score: 67.3958 bits (163), Expect = 2.067e-11
Identity = 56/160 (35.00%), Postives = 90/160 (56.25%), Query Frame = 1
Query:  445 VSLKILHISETRIEHLSRGMFSDCPITERI-RLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            + +K LH+S  +I HL  G F +   + +I RLS NR++ +  K  +   ++  L+L  NRL +I  L F    SL+ L L RN I+++ D +F  L +M+VL+L+ N L  +    L+GLESL  L+L+ NSI     + +   + L+ +NL  N L +
Sbjct:  154 LQIKDLHLSSNKIVHLEFGAFKNLAGSLQILRLSRNRLTHLPVKGLE-LPKLTQLELSRNRLRLIEGLTFQGLSSLEVLKLQRNNISKLTDGAFWGLARMRVLHLDYNSLREVNSGSLYGLESLLQLYLANNSISNFNPEGWGFCERLRELNLSYNNLTK 312          

HSP 2 Score: 53.1434 bits (126), Expect = 6.673e-7
Identity = 47/189 (24.87%), Postives = 94/189 (49.74%), Query Frame = 1
Query:  364 FKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXX---XXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            F+ +S LE L +Q   I  + +        +++LH+    +  ++ G         ++ L++N IS    + +  C R+  L+L  N LT +    F +  +L +L L  N I+ I + +F+ L  ++ L L+    +  +        GLE+L  L L +N+I +I+ +AF  +K+L+Y++++ +  L
Sbjct:  222 FQGLSSLEVLKLQRNNISKLTDGAFWGLARMRVLHLDYNSLREVNSGSLYGLESLLQLYLANNSISNFNPEGWGFCERLRELNLSYNNLTKLSEGSFAKLVNLISLRLGHNSISHITEGAFRGLSSLRTLELDHNDISGTIEDTSGAFTGLENLNKLNLGENAIRSIQPEAFSKMKNLRYLHIQSDSFL 410          
BLAST of SMED30003270 vs. Ensembl Zebrafish
Match: lrrtm4l1 (leucine rich repeat transmembrane neuronal 4 like 1 [Source:ZFIN;Acc:ZDB-GENE-080327-15])

HSP 1 Score: 66.2402 bits (160), Expect = 3.736e-11
Identity = 55/187 (29.41%), Postives = 91/187 (48.66%), Query Frame = 1
Query:  358 QMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            + F+ V RL+ L++ +  I  + N       +L+ L +S  +++ L  G F      + + L SN ++ I  + F  C  +  LDLG NRL V+ R  F     L  L L  N+ +RI  F F  L  ++ L L  N +  +   + +   +LQ L +S N I+ ++   F  L +L+ +NLE N L
Sbjct:  117 RAFQGVRRLKELILSSNRISQLHNVTFHGVPNLRSLDLSYNKLQELQPGQFYGLRKLQNLHLRSNGLTAIPVRAFLECRSLEFLDLGYNRLRVLTRTAFLGLSRLMELHLEHNQFSRINFFLFPRLANLRALYLQWNRIRAVNQGLPWSWYTLQRLDISGNEIQVLDPVVFQCLPNLQVLNLESNKL 303          
BLAST of SMED30003270 vs. Ensembl Zebrafish
Match: lrrn3a (leucine rich repeat neuronal 3a [Source:ZFIN;Acc:ZDB-GENE-061220-7])

HSP 1 Score: 65.855 bits (159), Expect = 5.130e-11
Identity = 64/263 (24.33%), Postives = 128/263 (48.67%), Query Frame = 1
Query:  133 CQKKCRCKHKEKSSKIEVAHCTVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            C K C+C+ +   S + +     +E  T V CN    + L+T+ + R  +D        + +L++  +S+  +   +  LV+  E  + +  I ++ + ++ +   L  LH+ E  +  L     +  P  + + ++ N +S+I+ + F   N+++ L L +N+L  IR   F +   L+ L +  N I RI++ +FK L  ++ L L    L  I    L GL+ L+ +    N    + + A   +++LK+++L KNP+ R
Sbjct:   29 CLKLCKCEIRPWFSPLSM----YNEAPT-VDCN---DLGLLTLPE-RLPSD--------TQVLLSQANSIAKIDSPLDYLVNLTEVDLSRNNISSLSDIYIGHIPQLLSLHLEENWLSSLHDNFLAHFPNLQELYVNHNLLSLISAEAFQGLNKLLRLHLNSNQLRAIRSEWFQDLSQLEILMIGENPIARIQNMNFKPLINLRSLVLTRMNLTEIPDSALVGLDKLESVSFYDNMFPKVPQAALRQVRNLKFLDLNKNPIER 274          
BLAST of SMED30003270 vs. Ensembl Xenopus
Match: IGFALS (insulin like growth factor binding protein acid labile subunit [Source:NCBI gene;Acc:100489984])

HSP 1 Score: 68.1662 bits (165), Expect = 1.272e-11
Identity = 52/189 (27.51%), Postives = 100/189 (52.91%), Query Frame = 1
Query:  346 VLMPQMFKLVSRLETLVV-QAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            +L+ + F+ +++LE L +    +Q I        +++ ++++S   ++ L+   F        + + S+ +S I  + F   + +  L L NN +  I    F + H L  LDL  N++T +   SF  LK +  L L++N ++ I PE+   ++ LQ+L LS N ++ + ED F+ L SL+Y++L+ N
Sbjct:  321 ILLERTFEGLAQLELLSLNHNNVQEIRPGSFLGLLNVAVINLSGNCLKSLAEHCFKGLGKLHSLHMESSCLSHIKPQMFAGLSSIRRLFLQNNEIVAIENHSFTDLHGLLELDLRSNKLTHLTTRSFTGLKNLSYLLLSSNQILTISPEVFSPVQQLQWLDLSDNQLKALTEDIFLPLSSLRYLSLKNN 509          

HSP 2 Score: 55.0694 bits (131), Expect = 2.166e-7
Identity = 49/193 (25.39%), Postives = 83/193 (43.01%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXX------------------------XXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            I T+  +      +L+ L +S  R+  L    F   P    +RLS+N ++ +  + F     +V L+LG N++ ++    F     L+ L L+ N +  I   SF  L  +                          L++ ++ L  IKP++  GL S++ LFL  N I  IE  +F +L  L  ++L  N L
Sbjct:  247 ISTVAPRAFSGMKNLRWLDLSHNRLTALYEDTFFGLPSLNVLRLSNNSLTSLRPRIFKDLQFLVELNLGQNKIKILLERTFEGLAQLELLSLNHNNVQEIRPGSFLGLLNVAVINLSGNCLKSLAEHCFKGLGKLHSLHMESSCLSHIKPQMFAGLSSIRRLFLQNNEIVAIENHSFTDLHGLLELDLRSNKL 439          

HSP 3 Score: 51.9878 bits (123), Expect = 1.730e-6
Identity = 53/193 (27.46%), Postives = 95/193 (49.22%), Query Frame = 1
Query:  337 SLIVLMPQMF--KLVSRLETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            +L+V++P M    L++  E ++    +  ++     +   LK L +S   ++ +   +F+    T ++ L+ N IS +A + F     +  LDL +NRLT +    F    SL  L LS N +T +    FK+L+ +  LNL  N +  +      GL  L+ L L+ N+++ I   +F+ L ++  INL  N
Sbjct:  173 NLLVVLPDMVFHDLLNLRELVLAGNRLVYLQPSLFLSLGELKELDLSGNSLKGIKANVFTRQQKTHKLYLNHNHISTVAPRAFSGMKNLRWLDLSHNRLTALYEDTFFGLPSLNVLRLSNNSLTSLRPRIFKDLQFLVELNLGQNKIKILLERTFEGLAQLELLSLNHNNVQEIRPGSFLGLLNVAVINLSGN 365          

HSP 4 Score: 49.6766 bits (117), Expect = 9.324e-6
Identity = 51/188 (27.13%), Postives = 84/188 (44.68%), Query Frame = 1
Query:  349 LMPQMFKLVSRLETLVVQAPIQT-IENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            L P  F     L +L +     T IE+       +L  L++    +  L   +F D      + L+ NR+  +    F S   +  LDL  N L  I+  +F        L L+ N I+ +   +F  +K ++ L+L+ N L  +  +  FGL SL  L LS NS+ ++    F +L+ L  +NL +N
Sbjct:  130 LAPSTFTHTQNLVSLTLNNNFLTKIEDGLFSGLSNLWYLNLGWNLLVVLPDMVFHDLLNLRELVLAGNRLVYLQPSLFLSLGELKELDLSGNSLKGIKANVFTRQQKTHKLYLNHNHISTVAPRAFSGMKNLRWLDLSHNRLTALYEDTFFGLPSLNVLRLSNNSLTSLRPRIFKDLQFLVELNLGQN 317          
BLAST of SMED30003270 vs. Ensembl Xenopus
Match: LRIG2 (leucine-rich repeats and immunoglobulin like domains 2 [Source:NCBI gene;Acc:100494845])

HSP 1 Score: 65.855 bits (159), Expect = 9.189e-11
Identity = 60/181 (33.15%), Postives = 94/181 (51.93%), Query Frame = 1
Query:  382 LETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSS-NRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            LETL + + + T    F    + LK L++S  RI  L  G F +   +  +   + NRI+VI  K+F     +  L+L  NR+ ++  L F    SL++L L RN I ++ D +F  L  M+ L L  N +  I    L+GL SLQ L++S+N++  I  DA+   + L  ++L  N L R
Sbjct:  129 LETLDLSSNLLTEIKSFYFPRMPLKYLNLSNNRIATLEAGCFDNLSSSLLVLKLNRNRINVIQPKSF-KLPHLQYLELRRNRIKIVESLTFQGLDSLKSLKLQRNGIVKLMDGAFFGLDNMEQLELEYNNVTDINKGWLYGLRSLQQLYISQNAVHRISPDAWEFCQKLLDLDLSYNQLNR 308          

HSP 2 Score: 58.151 bits (139), Expect = 2.422e-8
Identity = 51/197 (25.89%), Postives = 89/197 (45.18%), Query Frame = 1
Query:  334 SSLIVLMPQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            + ++ LM   F  +  +E L ++   +  I   +L    SL+ L+IS+  +  +S   +  C     + LS N+++ + D  F   + +  ++LG+NR+  I   +F    +L  LDL  N I+  IED +                      E+  GL  L  L L  N I++I   AF  L SL++++L  N +L
Sbjct:  232 NGIVKLMDGAFFGLDNMEQLELEYNNVTDINKGWLYGLRSLQQLYISQNAVHRISPDAWEFCQKLLDLDLSYNQLNRLDDFAFVGLSSLEKINLGDNRINHIAEGVFKGLANLLVLDLRNNEISWAIEDSN----------------------EVFAGLSRLHTLILQGNKIKSITMKAFTGLDSLQHLDLSNNAIL 406          
BLAST of SMED30003270 vs. Ensembl Xenopus
Match: LRIG2 (leucine-rich repeats and immunoglobulin like domains 2 [Source:NCBI gene;Acc:100494845])

HSP 1 Score: 65.855 bits (159), Expect = 1.005e-10
Identity = 60/181 (33.15%), Postives = 94/181 (51.93%), Query Frame = 1
Query:  382 LETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSS-NRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            LETL + + + T    F    + LK L++S  RI  L  G F +   +  +   + NRI+VI  K+F     +  L+L  NR+ ++  L F    SL++L L RN I ++ D +F  L  M+ L L  N +  I    L+GL SLQ L++S+N++  I  DA+   + L  ++L  N L R
Sbjct:   74 LETLDLSSNLLTEIKSFYFPRMPLKYLNLSNNRIATLEAGCFDNLSSSLLVLKLNRNRINVIQPKSF-KLPHLQYLELRRNRIKIVESLTFQGLDSLKSLKLQRNGIVKLMDGAFFGLDNMEQLELEYNNVTDINKGWLYGLRSLQQLYISQNAVHRISPDAWEFCQKLLDLDLSYNQLNR 253          

HSP 2 Score: 58.151 bits (139), Expect = 2.528e-8
Identity = 51/197 (25.89%), Postives = 89/197 (45.18%), Query Frame = 1
Query:  334 SSLIVLMPQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            + ++ LM   F  +  +E L ++   +  I   +L    SL+ L+IS+  +  +S   +  C     + LS N+++ + D  F   + +  ++LG+NR+  I   +F    +L  LDL  N I+  IED +                      E+  GL  L  L L  N I++I   AF  L SL++++L  N +L
Sbjct:  177 NGIVKLMDGAFFGLDNMEQLELEYNNVTDINKGWLYGLRSLQQLYISQNAVHRISPDAWEFCQKLLDLDLSYNQLNRLDDFAFVGLSSLEKINLGDNRINHIAEGVFKGLANLLVLDLRNNEISWAIEDSN----------------------EVFAGLSRLHTLILQGNKIKSITMKAFTGLDSLQHLDLSNNAIL 351          
BLAST of SMED30003270 vs. Ensembl Xenopus
Match: LRIG2 (leucine-rich repeats and immunoglobulin like domains 2 [Source:NCBI gene;Acc:100494845])

HSP 1 Score: 65.855 bits (159), Expect = 1.038e-10
Identity = 60/181 (33.15%), Postives = 94/181 (51.93%), Query Frame = 1
Query:  382 LETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSS-NRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            LETL + + + T    F    + LK L++S  RI  L  G F +   +  +   + NRI+VI  K+F     +  L+L  NR+ ++  L F    SL++L L RN I ++ D +F  L  M+ L L  N +  I    L+GL SLQ L++S+N++  I  DA+   + L  ++L  N L R
Sbjct:   61 LETLDLSSNLLTEIKSFYFPRMPLKYLNLSNNRIATLEAGCFDNLSSSLLVLKLNRNRINVIQPKSF-KLPHLQYLELRRNRIKIVESLTFQGLDSLKSLKLQRNGIVKLMDGAFFGLDNMEQLELEYNNVTDINKGWLYGLRSLQQLYISQNAVHRISPDAWEFCQKLLDLDLSYNQLNR 240          

HSP 2 Score: 58.151 bits (139), Expect = 2.414e-8
Identity = 51/197 (25.89%), Postives = 89/197 (45.18%), Query Frame = 1
Query:  334 SSLIVLMPQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            + ++ LM   F  +  +E L ++   +  I   +L    SL+ L+IS+  +  +S   +  C     + LS N+++ + D  F   + +  ++LG+NR+  I   +F    +L  LDL  N I+  IED +                      E+  GL  L  L L  N I++I   AF  L SL++++L  N +L
Sbjct:  164 NGIVKLMDGAFFGLDNMEQLELEYNNVTDINKGWLYGLRSLQQLYISQNAVHRISPDAWEFCQKLLDLDLSYNQLNRLDDFAFVGLSSLEKINLGDNRINHIAEGVFKGLANLLVLDLRNNEISWAIEDSN----------------------EVFAGLSRLHTLILQGNKIKSITMKAFTGLDSLQHLDLSNNAIL 338          
BLAST of SMED30003270 vs. Ensembl Xenopus
Match: nprl2 (NPR2 like, GATOR1 complex subunit [Source:Xenbase;Acc:XB-GENE-854704])

HSP 1 Score: 64.6994 bits (156), Expect = 2.084e-10
Identity = 47/189 (24.87%), Postives = 94/189 (49.74%), Query Frame = 1
Query:  349 LMPQMFKLVSRLETLVV-QAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFG-LESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            L P +F  +  LE L++ +  + ++ ++ LKN   L  L++S  +I HL   +FS     +++ L  N++  I    F+    +++L L +N +  + +  F     LQ+L LS+N++ ++  +               +  ++  P++LFG +E+L  L+L    + TI    F NL +L+ + L +N
Sbjct:  136 LHPNLFCCLQHLEELILNRNQLTSLPDELLKNLTKLISLNLSRNKISHLPMSIFSSLTKLKKLFLYDNQLVTITSSAFNKLGELLDLALYSNSIQFVAQDAFYHLPKLQSLKLSKNKL-QLLPYGLFLHLPQLSTLTLYDNPLKELPDVLFGKMENLTSLWLYNTHLTTIPNFVFCNLTNLQLLVLTRN 323          

HSP 2 Score: 51.6026 bits (122), Expect = 2.509e-6
Identity = 52/169 (30.77%), Postives = 83/169 (49.11%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            + +I     K+F  LK L +++ +++ L  GMF      E++ L  N +S +    F     +  L L  N+LT +   +      L +L+LSRN+I+ +    F +L K+K L L  N L+ I       L  L  L L  NSI+ + +DAF +L  L+ + L KN L
Sbjct:   85 LTSITRNAFKSFPHLKSLKLTDNKLKTLPAGMFDSLISLEQLFLDKNCLSSLHPNLFCCLQHLEELILNRNQLTSLPDELLKNLTKLISLNLSRNKISHLPMSIFSSLTKLKKLFLYDNQLVTITSSAFNKLGELLDLALYSNSIQFVAQDAFYHLPKLQSLKLSKNKL 253          

HSP 3 Score: 50.447 bits (119), Expect = 5.921e-6
Identity = 36/150 (24.00%), Postives = 69/150 (46.00%), Query Frame = 1
Query:  460 LHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            +HI ET    ++   F + P+T R+RL +N ++ I    F S   + +L L +N+L  +   +F+   SL+ L L +N ++ +                         P +   L+ L+ L L++N + ++ ++   NL  L  +NL +N
Sbjct:   54 IHIMETLATEITDKSFGNMPVTLRLRLEANHLTSITRNAFKSFPHLKSLKLTDNKLKTLPAGMFDSLISLEQLFLDKNCLSSLH------------------------PNLFCCLQHLEELILNRNQLTSLPDELLKNLTKLISLNLSRN 179          
BLAST of SMED30003270 vs. Ensembl Mouse
Match: Lrig1 (leucine-rich repeats and immunoglobulin-like domains 1 [Source:MGI Symbol;Acc:MGI:107935])

HSP 1 Score: 70.0922 bits (170), Expect = 3.986e-12
Identity = 61/195 (31.28%), Postives = 93/195 (47.69%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCN------------------------RVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            I +++   LK+++SL++L +S   I  +    F +      + L+SNRIS++    FD  +                        R+  LDL  NR+ +I  L F    SL+ L L RN I+R+ D +F  L KM VL+L  N L+ +    L+GL +L  L LS NSI  I+ D +   + L  + L  N L R
Sbjct:  129 ILSVDGSQLKSYLSLEVLDLSSNNITEIRSSCFPNGLRIRELNLASNRISILESGAFDGLSRSLLTLRLSKNRITQLPVKAFKLPRLTQLDLNRNRIRLIEGLTFQGLDSLEVLRLQRNNISRLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSISRIQRDGWSFCQKLHELILSFNNLTR 323          

HSP 2 Score: 51.9878 bits (123), Expect = 1.557e-6
Identity = 51/162 (31.48%), Postives = 82/162 (50.62%), Query Frame = 1
Query:  433 LKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFS--FXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLK--YINLE 903
            L    +L  LH+S   I  + R  +S C     + LS N ++ + +++    + +  L L +N ++ I    F    SL+ LDL  N I+  IED S  F  L  +  L L  N +  +      GLESL++L L +N+I +++ DAF  +K+LK  YI+ E
Sbjct:  281 LYGLTALHQLHLSNNSISRIQRDGWSFCQKLHELILSFNNLTRLDEESLAELSSLSILRLSHNAISHIAEGAFKGLKSLRVLDLDHNEISGTIEDTSGAFTGLDNLSKLTLFGNKIKSVAKRAFSGLESLEHLNLGENAIRSVQFDAFAKMKNLKELYISSE 442          
BLAST of SMED30003270 vs. Ensembl Mouse
Match: Lrig1 (leucine-rich repeats and immunoglobulin-like domains 1 [Source:MGI Symbol;Acc:MGI:107935])

HSP 1 Score: 70.0922 bits (170), Expect = 3.986e-12
Identity = 61/195 (31.28%), Postives = 93/195 (47.69%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCN------------------------RVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            I +++   LK+++SL++L +S   I  +    F +      + L+SNRIS++    FD  +                        R+  LDL  NR+ +I  L F    SL+ L L RN I+R+ D +F  L KM VL+L  N L+ +    L+GL +L  L LS NSI  I+ D +   + L  + L  N L R
Sbjct:  129 ILSVDGSQLKSYLSLEVLDLSSNNITEIRSSCFPNGLRIRELNLASNRISILESGAFDGLSRSLLTLRLSKNRITQLPVKAFKLPRLTQLDLNRNRIRLIEGLTFQGLDSLEVLRLQRNNISRLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSISRIQRDGWSFCQKLHELILSFNNLTR 323          

HSP 2 Score: 51.9878 bits (123), Expect = 1.557e-6
Identity = 51/162 (31.48%), Postives = 82/162 (50.62%), Query Frame = 1
Query:  433 LKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFS--FXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLK--YINLE 903
            L    +L  LH+S   I  + R  +S C     + LS N ++ + +++    + +  L L +N ++ I    F    SL+ LDL  N I+  IED S  F  L  +  L L  N +  +      GLESL++L L +N+I +++ DAF  +K+LK  YI+ E
Sbjct:  281 LYGLTALHQLHLSNNSISRIQRDGWSFCQKLHELILSFNNLTRLDEESLAELSSLSILRLSHNAISHIAEGAFKGLKSLRVLDLDHNEISGTIEDTSGAFTGLDNLSKLTLFGNKIKSVAKRAFSGLESLEHLNLGENAIRSVQFDAFAKMKNLKELYISSE 442          
BLAST of SMED30003270 vs. Ensembl Mouse
Match: Lrig1 (leucine-rich repeats and immunoglobulin-like domains 1 [Source:MGI Symbol;Acc:MGI:107935])

HSP 1 Score: 70.0922 bits (170), Expect = 3.986e-12
Identity = 61/195 (31.28%), Postives = 93/195 (47.69%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCN------------------------RVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            I +++   LK+++SL++L +S   I  +    F +      + L+SNRIS++    FD  +                        R+  LDL  NR+ +I  L F    SL+ L L RN I+R+ D +F  L KM VL+L  N L+ +    L+GL +L  L LS NSI  I+ D +   + L  + L  N L R
Sbjct:  129 ILSVDGSQLKSYLSLEVLDLSSNNITEIRSSCFPNGLRIRELNLASNRISILESGAFDGLSRSLLTLRLSKNRITQLPVKAFKLPRLTQLDLNRNRIRLIEGLTFQGLDSLEVLRLQRNNISRLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSISRIQRDGWSFCQKLHELILSFNNLTR 323          

HSP 2 Score: 51.9878 bits (123), Expect = 1.557e-6
Identity = 51/162 (31.48%), Postives = 82/162 (50.62%), Query Frame = 1
Query:  433 LKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFS--FXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLK--YINLE 903
            L    +L  LH+S   I  + R  +S C     + LS N ++ + +++    + +  L L +N ++ I    F    SL+ LDL  N I+  IED S  F  L  +  L L  N +  +      GLESL++L L +N+I +++ DAF  +K+LK  YI+ E
Sbjct:  281 LYGLTALHQLHLSNNSISRIQRDGWSFCQKLHELILSFNNLTRLDEESLAELSSLSILRLSHNAISHIAEGAFKGLKSLRVLDLDHNEISGTIEDTSGAFTGLDNLSKLTLFGNKIKSVAKRAFSGLESLEHLNLGENAIRSVQFDAFAKMKNLKELYISSE 442          
BLAST of SMED30003270 vs. Ensembl Mouse
Match: Lrig3 (leucine-rich repeats and immunoglobulin-like domains 3 [Source:MGI Symbol;Acc:MGI:2443955])

HSP 1 Score: 67.3958 bits (163), Expect = 2.587e-11
Identity = 58/194 (29.90%), Postives = 101/194 (52.06%), Query Frame = 1
Query:  349 LMPQMFKLVSRLETLVV-QAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFS--FXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            LM   F  +S +E L +    +  I   +L   + L+ LH+S+  I  +S   +  C     + L+ N +S + D +F   + +  L +GNN+++ I    F    SL+ LDL  N I+  IED S  F  L +++ L L  N +  I  +   GL++L++L LS N+I +++ +AF  +K L+ ++L  + LL
Sbjct:  254 LMDGAFWGLSNMEVLQLDHNNLTEITKGWLYGLLMLRELHLSQNAINRISPDAWEFCQKLSELDLTFNHLSRLDDSSFLGLSLLNALHIGNNKVSYIADCAFRGLTSLKTLDLRNNEISWTIEDMSGAFSGLDRLRQLILQGNRIRSITKKAFAGLDTLEHLDLSGNAIMSLQSNAFSQMKKLQQLHLNTSSLL 447          
BLAST of SMED30003270 vs. Ensembl Mouse
Match: Slit1 (slit guidance ligand 1 [Source:MGI Symbol;Acc:MGI:1315203])

HSP 1 Score: 67.3958 bits (163), Expect = 2.715e-11
Identity = 45/156 (28.85%), Postives = 72/156 (46.15%), Query Frame = 1
Query:  451 LKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            L++L + E +I  + RG F D    ER+RL+ N++ V+ +  F +   +  LDL  N L  + R  F     L+NL L +NRI+ IE+ +F+                         L  L+ L L+ N+I TI   +F ++  L+   L  N L 
Sbjct:   87 LRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLQVLPELLFQNNQALSRLDLSENFLQAVPRKAFRGATDLKNLQLDKNRISCIEEGAFR------------------------ALRGLEVLTLNNNNITTIPVSSFNHMPKLRTFRLHSNHLF 218          

HSP 2 Score: 58.151 bits (139), Expect = 1.824e-8
Identity = 44/139 (31.65%), Postives = 62/139 (44.60%), Query Frame = 1
Query:  499 GMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            G+F      ++I LS+N++S I D TF+    V  L L  N+L  IR  +F     L+ L L  NRI+ I + SF                         GL +++ L L  N I TI   AF  L++L  +NL  NP 
Sbjct:  559 GLFKKLSHLKKINLSNNKVSEIEDGTFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHNDSFT------------------------GLRNVRLLSLYDNHITTISPGAFDTLQALSTLNLLANPF 673          
BLAST of SMED30003270 vs. UniProt/SwissProt
Match: sp|P35858|ALS_HUMAN (Insulin-like growth factor-binding protein complex acid labile subunit OS=Homo sapiens OX=9606 GN=IGFALS PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 2.176e-15
Identity = 58/186 (31.18%), Postives = 99/186 (53.23%), Query Frame = 1
Query:  355 PQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            P  F+ +S L  L +Q   + ++E Q L    +L  LH+   ++  L+ G F+  P    + LS+NR+S + D  F+    + +L+LG N L V+    F    SL+ L L+ NR+  ++   F  L +++ L+L+ N L  IK  +   L  LQ L+L +N I  +   AF+ LK+L++++L  N
Sbjct:   91 PAAFQNLSSLGFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLALGTFAHTPALASLGLSNNRLSRLEDGLFEGLGSLWDLNLGWNSLAVLPDAAFRGLGSLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHN 276          

HSP 2 Score: 52.7582 bits (125), Expect = 5.902e-6
Identity = 44/149 (29.53%), Postives = 74/149 (49.66%), Query Frame = 1
Query:  469 SETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            S   I  L    F D    E ++L  NRI  +A+++F+   ++  L L +N+L  ++   F    ++  ++LS N +  + +  F+ L K+  L+L  + L RI+P    GL  L+ LFL  N +  IEE +   L  L  ++L  N L
Sbjct:  298 SHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFTGLSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQL 446          

HSP 3 Score: 51.9878 bits (123), Expect = 9.035e-6
Identity = 54/221 (24.43%), Postives = 94/221 (42.53%), Query Frame = 1
Query:  334 SSLIVLMPQMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNR------------------------LTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            +SL VL    F+ +  L  LV+    +  ++         L+ L +S   +  +   +F   P  +++ L  N I+ +A   F     +  LDL +NR                        +  +R   F + H L+ L L  NRI ++ + SF+ L +++VL L+ N L  +K     GL ++  + LS N +  + E  F  L  L  ++LE + L R
Sbjct:  180 NSLAVLPDAAFRGLGSLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFVQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFKDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKAGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGR 400          
BLAST of SMED30003270 vs. UniProt/SwissProt
Match: sp|O02833|ALS_PAPHA (Insulin-like growth factor-binding protein complex acid labile subunit OS=Papio hamadryas OX=9557 GN=IGFALS PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.414e-14
Identity = 57/186 (30.65%), Postives = 98/186 (52.69%), Query Frame = 1
Query:  355 PQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            P  F+ +S L  L +Q   + ++E Q L    +L  LH+   ++  L+ G F+  P    + LS+NR+S + D  F+    + +L+LG N L V+    F     L+ L L+ NR+  ++   F  L +++ L+L+ N L  IK  +   L  LQ L+L +N I  +   AF+ LK+L++++L  N
Sbjct:   91 PAAFRNLSSLAFLNLQGGQLGSLEPQALLGLENLCHLHLERNQLRSLAVGTFAYTPALALLGLSNNRLSRLEDGLFEGLGNLWDLNLGWNSLAVLPDAAFRGLGGLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFAQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHN 276          

HSP 2 Score: 52.373 bits (124), Expect = 6.902e-6
Identity = 54/221 (24.43%), Postives = 95/221 (42.99%), Query Frame = 1
Query:  334 SSLIVLMPQMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNR------------------------LTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            +SL VL    F+ +  L  LV+    +  ++         L+ L +S   +  +   +F+  P  +++ L  N I+ +A   F     +  LDL +NR                        +  +R   F + H L+ L L  NRI ++ + SF+ L +++VL L+ N L  +K     GL ++  + LS N +  + E  F  L  L  ++LE + L R
Sbjct:  180 NSLAVLPDAAFRGLGGLRELVLAGNRLAYLQPALFSGLAELRELDLSRNALRAIKANVFAQLPRLQKLYLDRNLIAAVAPGAFLGLKALRWLDLSHNRVAGLLEDTFPGLLGLRVLRLSHNAIASLRPRTFEDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKVGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGR 400          

HSP 3 Score: 52.373 bits (124), Expect = 7.931e-6
Identity = 44/149 (29.53%), Postives = 74/149 (49.66%), Query Frame = 1
Query:  469 SETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            S   I  L    F D    E ++L  NRI  +A+++F+   ++  L L +N+L  ++   F    ++  ++LS N +  + +  F+ L K+  L+L  + L RI+P    GL  L+ LFL  N +  IEE +   L  L  ++L  N L
Sbjct:  298 SHNAIASLRPRTFEDLHFLEELQLGHNRIRQLAERSFEGLGQLEVLTLDHNQLQEVKVGAFLGLTNVAVMNLSGNCLRNLPEQVFRGLGKLHSLHLEGSCLGRIRPHTFAGLSGLRRLFLKDNGLVGIEEQSLWGLAELLELDLTSNQL 446          
BLAST of SMED30003270 vs. UniProt/SwissProt
Match: sp|P70193|LRIG1_MOUSE (Leucine-rich repeats and immunoglobulin-like domains protein 1 OS=Mus musculus OX=10090 GN=Lrig1 PE=1 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 2.788e-11
Identity = 61/195 (31.28%), Postives = 93/195 (47.69%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCN------------------------RVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            I +++   LK+++SL++L +S   I  +    F +      + L+SNRIS++    FD  +                        R+  LDL  NR+ +I  L F    SL+ L L RN I+R+ D +F  L KM VL+L  N L+ +    L+GL +L  L LS NSI  I+ D +   + L  + L  N L R
Sbjct:  129 ILSVDGSQLKSYLSLEVLDLSSNNITEIRSSCFPNGLRIRELNLASNRISILESGAFDGLSRSLLTLRLSKNRITQLPVKAFKLPRLTQLDLNRNRIRLIEGLTFQGLDSLEVLRLQRNNISRLTDGAFWGLSKMHVLHLEYNSLVEVNSGSLYGLTALHQLHLSNNSISRIQRDGWSFCQKLHELILSFNNLTR 323          
BLAST of SMED30003270 vs. UniProt/SwissProt
Match: sp|P24014|SLIT_DROME (Protein slit OS=Drosophila melanogaster OX=7227 GN=sli PE=1 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 2.849e-11
Identity = 55/213 (25.82%), Postives = 101/213 (47.42%), Query Frame = 1
Query:  292 AACQRI---SGLLITTESSLIVLMPQMFKLVSRLETLVVQAPIQTI--ENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            A C R+   +GL +      +  +P+  K+ + +E L +Q    T+  E  F +    L++L +++ +I  + R  F D    ER+RL++NR+  I +    S   ++ LD+ NN +T + R +F    SL++L L  N+IT +++ +FK                        GL  L+ L L+ N++ ++  + F  L  L+ + L  NP 
Sbjct:   71 ARCPRVCSCTGLNVDCSHRGLTSVPR--KISADVERLELQGNNLTVIYETDF-QRLTKLRMLQLTDNQIHTIERNSFQDLVSLERLRLNNNRLKAIPENFVTSSASLLRLDISNNVITTVGRRVFKGAQSLRSLQLDNNQITCLDEHAFK------------------------GLVELEILTLNNNNLTSLPHNIFGGLGRLRALRLSDNPF 256          
BLAST of SMED30003270 vs. UniProt/SwissProt
Match: sp|P35859|ALS_RAT (Insulin-like growth factor-binding protein complex acid labile subunit OS=Rattus norvegicus OX=10116 GN=Igfals PE=1 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 7.181e-11
Identity = 53/183 (28.96%), Postives = 101/183 (55.19%), Query Frame = 1
Query:  364 FKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            F+ +S L+ L +Q   ++++E Q L    +L  LH+   R+ +L+ G+F+  P    + LSSN +  + +  F   + + +L+LG N L V+   +F    +L  L L+ N++T ++   F  L +++ L+L+ N L  +K  +   L  LQ L+L +N I  +   AF+ +K+L++++L  N
Sbjct:   94 FQNLSSLDFLNLQGSWLRSLEPQALLGLQNLYYLHLERNRLRNLAVGLFTHTPSLASLSLSSNLLGRLEEGLFQGLSHLWDLNLGWNSLVVLPDTVFQGLGNLHELVLAGNKLTYLQPALFCGLGELRELDLSRNALRSVKANVFVHLPRLQKLYLDRNLITAVAPGAFLGMKALRWLDLSHN 276          

HSP 2 Score: 58.9214 bits (141), Expect = 5.544e-8
Identity = 47/166 (28.31%), Postives = 84/166 (50.60%), Query Frame = 1
Query:  418 IENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            +E+ F    + L +L ++   I  L    F D    E ++L  NRI  + ++TF+   ++  L L +N++T +R   F+   ++  ++LS N +  + +  F+ L K+  L+L  + L  ++     GL  L+ LFL  NSI +IEE +   L  L  ++L  N L
Sbjct:  282 MEDTF-PGLLGLHVLRLAHNAIASLRPRTFKDLHFLEELQLGHNRIRQLGERTFEGLGQLEVLTLNDNQITEVRVGAFSGLFNVAVMNLSGNCLRSLPERVFQGLDKLHSLHLEHSCLGHVRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTTNRL 446          

HSP 3 Score: 54.6842 bits (130), Expect = 1.254e-6
Identity = 41/169 (24.26%), Postives = 76/169 (44.97%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            ++++  +  +    L  LH+  + + H+    F+      R+ L  N IS I +++    + ++ LDL  NRLT + R +F     L+ L LS N++T                         +  E+L  L+   +L +S N +ET+ E  F +L  ++Y++L  N L
Sbjct:  374 LRSLPERVFQGLDKLHSLHLEHSCLGHVRLHTFAGLSGLRRLFLRDNSISSIEEQSLAGLSELLELDLTTNRLTHLPRQLFQGLGHLEYLLLSYNQLT------------------------TLSAEVLGPLQRAFWLDISHNHLETLAEGLFSSLGRVRYLSLRNNSL 518          
BLAST of SMED30003270 vs. TrEMBL
Match: A0A3Q1FV13 (Insulin-like growth factor binding protein, acid labile subunit OS=Acanthochromis polyacanthus OX=80966 PE=4 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 7.048e-16
Identity = 75/263 (28.52%), Postives = 131/263 (49.81%), Query Frame = 1
Query:  130 PCQKKCRCKHKEKSSKIEVAHCTVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            PC K C C H + SS++ + +C+      F     + P    +   L  + +L  +   +S                 F+ +S L+ L +Q+  + TI+ Q  K   SL  +H+   R+  L   +F + P    + L +N++S I ++ F   + +  L+LG N LTV+  + F++   L+ L L+ NR+  ++   F+NL ++K L+L  N L  IK  +   L  LQ L+L++N I T+   AFV +KSL++++L  N L
Sbjct:   34 PCSKGCTCLHDDYSSELNM-YCSARN---FTQVPTDMPT---STHSLWLDGNLFTSLPAVS-----------------FRDLSNLDFLNLQSGQLMTIDAQAFKGLRSLAHIHLERNRLRGLPGAIFQNTPNLASLSLHNNQLSRIEERLFAGLSHMWLLNLGWNSLTVLPEMAFHDLQGLRELILAGNRLAYLQPQLFQNLVELKELDLTGNSLKNIKANVFVKLTKLQKLYLAQNQIVTVVPRAFVGMKSLRWLDLTNNKL 272          
BLAST of SMED30003270 vs. TrEMBL
Match: A0A3B3YX16 (Insulin-like growth factor binding protein, acid labile subunit OS=Poecilia mexicana OX=48701 PE=4 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 8.196e-15
Identity = 74/263 (28.14%), Postives = 132/263 (50.19%), Query Frame = 1
Query:  130 PCQKKCRCKHKEKSSKIEVAHCTVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            PC K C C H + S+++ + +C+     T V  ++      + +    F   L AAC                     F+ +S L+ L +Q+  + T+++Q  +   SL  LHI   R+  L   +F + P    + L +N IS I ++ F   + +  L+LG N LTV+   +F++   L+ L+L+ NR+  ++   F+NL ++K L+L  N +  IK  +   L  LQ L+L++N I ++   AFV +KSL+++++  N L
Sbjct:   34 PCAKGCNCMHDDYSAEL-IVYCSA-RNLTQVPADIPPSTHYLWLDGNLF-VSLPAAC---------------------FRDLSNLDFLNLQSGQLLTLDHQTFRGLRSLAHLHIERNRLRGLPGSIFQNTPNLASLSLHNNHISRIDERLFAGLSHMWFLNLGWNSLTVLPETVFHDLQGLRELNLAGNRLAYLQPALFQNLGELKELDLTGNYIKVIKANVFVKLHKLQKLYLAQNQIGSVVPRAFVGMKSLRWLDMRNNKL 272          
BLAST of SMED30003270 vs. TrEMBL
Match: A0A087YST5 (Insulin-like growth factor binding protein, acid labile subunit OS=Poecilia formosa OX=48698 PE=4 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 9.207e-15
Identity = 74/263 (28.14%), Postives = 132/263 (50.19%), Query Frame = 1
Query:  130 PCQKKCRCKHKEKSSKIEVAHCTVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            PC K C C H + S+++ + +C+     T V  ++      + +    F   L AAC                     F+ +S L+ L +Q+  + T+++Q  +   SL  LHI   R+  L   +F + P    + L +N IS I ++ F   + +  L+LG N LTV+   +F++   L+ L+L+ NR+  ++   F+NL ++K L+L  N +  IK  +   L  LQ L+L++N I ++   AFV +KSL+++++  N L
Sbjct:   34 PCAKGCNCMHDDYSTEL-IVYCSA-RNLTQVPADIPPSTHYLWLDGNLF-VSLPAAC---------------------FRDLSNLDFLNLQSGQLLTLDHQTFRGLRSLAHLHIERNRLRGLPGSIFQNTPNLASLSLHNNHISRIDERLFAGLSHMWFLNLGWNSLTVLPETVFHDLQGLRELNLAGNRLAYLQPALFQNLGELKELDLTGNYIKVIKANVFVKLHKLQKLYLAQNQIGSVVPRAFVGMKSLRWLDMRNNKL 272          
BLAST of SMED30003270 vs. TrEMBL
Match: A0A3B3TLZ0 (Insulin-like growth factor binding protein, acid labile subunit OS=Poecilia latipinna OX=48699 PE=4 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 9.289e-15
Identity = 74/263 (28.14%), Postives = 132/263 (50.19%), Query Frame = 1
Query:  130 PCQKKCRCKHKEKSSKIEVAHCTVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            PC K C C H + S+++ + +C+     T V  ++      + +    F   L AAC                     F+ +S L+ L +Q+  + T+++Q  +   SL  LHI   R+  L   +F + P    + L +N IS I ++ F   + +  L+LG N LTV+   +F++   L+ L+L+ NR+  ++   F+NL ++K L+L  N +  IK  +   L  LQ L+L++N I ++   AFV +KSL+++++  N L
Sbjct:   34 PCAKGCNCMHDDYSTEL-IVYCSA-RNLTQVPADIPPSTHYLWLDGNLF-VSLPAAC---------------------FRDLSNLDFLNLQSGQLLTLDHQTFRGLRSLAHLHIERNRLRGLPGSIFQNTPNLASLSLHNNHISRIDERLFAGLSHMWFLNLGWNSLTVLPETVFHDLQGLRELNLAGNRLAYLQPALFQNLGELKELDLTGNYIKVIKANVFVKLHKLQKLYLAQNQIGSVVPRAFVGMKSLRWLDMRNNKL 272          
BLAST of SMED30003270 vs. TrEMBL
Match: A0A1S3R9S0 (insulin-like growth factor-binding protein complex acid labile subunit OS=Salmo salar OX=8030 GN=LOC106601095 PE=4 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 1.561e-14
Identity = 79/268 (29.48%), Postives = 128/268 (47.76%), Query Frame = 1
Query:  130 PCQKKCRCKHKEKSSKIEVAHC-----TVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            PC K C C H + S ++ V +C     T    D  VS    W                      + G L TT      L    FK +S L+ L +Q+  + ++++Q  +   SL  +H+   RI  L   +F + P    + L +N++S I +K F   + +  L+LG N L V+    F++ H L+ L L+ NR+  ++   F+ L ++K L+L  N L  IK  +   L  LQ L+L++N I T+   AFV +KSL++++L  N L
Sbjct:   34 PCSKGCTCLHDDYSLELNV-YCSARNYTQAPSDVPVSTRSLW----------------------LDGNLFTT------LPATAFKDLSNLDFLNLQSGQLVSLDSQVFRGLKSLAHIHLERNRIRSLPGTIFQNTPNLASLSLHNNQLSRIEEKMFAGLSHMWLLNLGWNSLAVLPETGFHDLHGLRELVLAGNRLAYLQPQLFQGLTELKELDLTGNYLKVIKANVFMKLPKLQKLYLAQNQIVTVVPRAFVGMKSLRWLDLTNNKL 272          

HSP 2 Score: 65.4698 bits (158), Expect = 1.512e-7
Identity = 56/200 (28.00%), Postives = 96/200 (48.00%), Query Frame = 1
Query:  319 LITTESSLIVLMPQMFKLVSRLETL-VVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            L   ++ ++ ++P+ F  +  L  L +    +  + ++      SL +L +S   I  L    F D    E +RLS NRI  +AD+ F+    +  L+L +N++   R   F     L  ++LS +    + D  FK L K+  L+L+   L R+  +   GL  L+ LFL  N+I  +E  +FV L  L+ ++L  N L
Sbjct:  241 LYLAQNQIVTVVPRAFVGMKSLRWLDLTNNKLSALHDETFLGLHSLHVLRLSNNSISGLRPRTFRDLQYLEELRLSYNRIQALADRIFEGLGHLEVLELEHNQVQEARMGTFMGLSHLAVINLSGSCFRSLPDQVFKGLSKLHSLHLDKGCLTRVTTQAFIGLSGLRRLFLQNNNISVVERQSFVELLGLQQLDLRFNKL 440          
BLAST of SMED30003270 vs. Ensembl Cavefish
Match: igfals (insulin like growth factor binding protein acid labile subunit [Source:NCBI gene;Acc:103036545])

HSP 1 Score: 83.9593 bits (206), Expect = 8.090e-17
Identity = 74/265 (27.92%), Postives = 134/265 (50.57%), Query Frame = 1
Query:  130 PCQKKCRCKHKEKSSKIEVAHCTVDEKDTFVSCNVEW--PILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            PC K+C C   + +S++ V +C+        S N+    P L  + + L  + +L A                  L    FK ++ L+ L +Q+  + ++++Q  K   +L  LH+    +  L   +F + P    + L +N++S I ++ F   + +  L+LG N L+V+    F++ H L+ L LS NR+  ++   F+ L ++K L+L+ N L  IK  +   L  LQ L+L++N I T+   AFV +KSL++++L +N L
Sbjct:   32 PCSKRCTCLLDDYTSELSV-YCS--------SRNLTQAPPDLPASTRSLWLDGNLFA-----------------TLPADSFKNLTNLDFLNLQSGQLTSLDSQVFKRLHNLAHLHLERNSLRILPAAIFQNTPNLATLNLHNNQLSRIEERLFAGLSHMWLLNLGWNSLSVLPETGFHDLHGLRELVLSGNRLAYLQPQLFQGLTELKELDLSGNQLRVIKANVFLKLPKLQKLYLAQNQITTVAPRAFVGMKSLRWLDLSRNRL 270          

HSP 2 Score: 56.9954 bits (136), Expect = 3.135e-8
Identity = 51/190 (26.84%), Postives = 90/190 (47.37%), Query Frame = 1
Query:  334 SSLIVLMPQMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINL 900
            +SL VL    F  +  L  LV+    +  ++ Q  +    LK L +S  ++  +   +F   P  +++ L+ N+I+ +A + F     +  LDL  NRLT++    F   HSL  L LS N I  +   +F++L+ ++ L++  N +  +   +  GL  L+ L L  N ++      F+ L  L  I L
Sbjct:  172 NSLSVLPETGFHDLHGLRELVLSGNRLAYLQPQLFQGLTELKELDLSGNQLRVIKANVFLKLPKLQKLYLAQNQITTVAPRAFVGMKSLRWLDLSRNRLTILHDETFLGLHSLHVLRLSNNSINSLRPGTFRDLQYLEELDMCHNQIRALGERVFEGLGHLEVLSLEYNKLQDARSGTFMGLSHLAVIKL 361          

HSP 3 Score: 52.373 bits (124), Expect = 8.933e-7
Identity = 47/197 (23.86%), Postives = 94/197 (47.72%), Query Frame = 1
Query:  328 TESSLIVLMPQMFKLVSRLETL-VVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            + +S+  L P  F+ +  LE L +    I+ +  +  +    L++L +   +++    G F        I+L+ +    + D+ F   +++ ++ L    LT +    F    SL+ L L  N I+ ++  SF  L+ ++ L++  N L  +     +GL++L+YL LSKN I     +  + L+ L +++L  N L
Sbjct:  290 SNNSINSLRPGTFRDLQYLEELDMCHNQIRALGERVFEGLGHLEVLSLEYNKLQDARSGTFMGLSHLAVIKLTGSCFRSLPDQVFKGLSKLHSMHLDKGCLTKVSTQGFIGLASLRRLFLQHNNISAVDRQSFVELQGLQQLDMRFNNLASLTSHTFYGLKALEYLLLSKNLIRHFPSEVLLPLQQLSWLDLSGNKL 486          
BLAST of SMED30003270 vs. Ensembl Cavefish
Match: lrrn3a (leucine rich repeat neuronal 3 [Source:NCBI gene;Acc:103039721])

HSP 1 Score: 73.9442 bits (180), Expect = 1.341e-13
Identity = 56/184 (30.43%), Postives = 92/184 (50.00%), Query Frame = 1
Query:  370 LVSRLETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            LV+  E  V Q  +  + +  + +   L  LH+ E RI  L    FS  P  + + ++ N +S+I+D  F     ++ L L +N+L VIR   FN  + L+ L +  N I +I+D +FK L  +  L L    L  I    L GL +L+ +    N    + + A   ++SLK+++L KNP+ R
Sbjct:   91 LVNLTEVDVSQNNVTRLSDLNVGHLPQLLSLHMEENRISTLHDNSFSHLPNLQELYVNHNLLSLISDGAFQGLGNLLRLHLNSNQLRVIRPEWFNALNQLEILMIGENPIAQIQDMNFKPLSNLLSLVLARMNLTEIPDNALVGLNNLESISFYDNKFAKVPQSALRKVQSLKFLDLNKNPIER 274          
BLAST of SMED30003270 vs. Ensembl Cavefish
Match: ENSAMXT00000025596.2 (pep primary_assembly:Astyanax_mexicanus-2.0:1:16543202:16552825:-1 gene:ENSAMXG00000024870.2 transcript:ENSAMXT00000025596.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 73.1738 bits (178), Expect = 2.841e-13
Identity = 56/197 (28.43%), Postives = 93/197 (47.21%), Query Frame = 1
Query:  328 TESSLIVLMPQMFKLVSRLETL-VVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            + S L VL P +F+ V +L+ L +    + ++      N   L+ L + + +I  +   +F      + + + SN IS +   TF +  R+V++DL NN+LT I   +F     L +LDLS+N +T +   +FK                         +  L  LFL KN+I ++ E+AF  L  LK ++L  NPL
Sbjct:  323 SSSKLQVLPPSVFQDVGKLKHLCLFDIALTSLPQGLFNNLTLLQELQLDKNKIHIIPPDLFHPLSKLKHLHMDSNLISELNSSTFRTLRRLVHIDLSNNQLTNISSGLFKGTRKLAHLDLSKNMLTAVPTEAFKE------------------------IPRLNRLFLQKNAISSVPENAFSGLAGLKKLDLSNNPL 495          

HSP 2 Score: 72.7886 bits (177), Expect = 3.962e-13
Identity = 52/169 (30.77%), Postives = 81/169 (47.93%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            I+ +  +FL+   +LKIL +S   I  LS G F      E I L+ N I +I    F++C  +  L L NN L+ +    F   H L++LDLS+NR++ +                     ++  P       +L  L+L +N I  + E+AF +L SL+ +NL  NPL
Sbjct:  159 IKILPQKFLQGLTNLKILVLSLNEILSLSYGAFEGGSDLEEIHLNKNHIKIIEKGVFENCTYLNKLVLSNNNLSSVEEGTFLGLHGLRHLDLSKNRLSTVP-----------------TGTLKDTP-------NLTSLYLHQNGITRVPENAFYSLSSLQMLNLNNNPL 303          
BLAST of SMED30003270 vs. Ensembl Cavefish
Match: lrig3 (leucine rich repeats and immunoglobulin like domains 3 [Source:NCBI gene;Acc:103037077])

HSP 1 Score: 71.2478 bits (173), Expect = 1.244e-12
Identity = 58/158 (36.71%), Postives = 88/158 (55.70%), Query Frame = 1
Query:  451 LKILHISETRIEHLSRGMFSDCPITERI-RLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            LK L+++  RI  L  G  ++   T  + RL+ NR++ I  KTF   N + +LDL  NR+  I  L F+  H L++L + RN ITR+ D +F  L  M++L L  N L  +    L+GL +LQ L L+ N+I  I+ DA+   + L  ++L  N L R
Sbjct:  154 LKNLYLNNNRISVLEHGCLNNLSSTLLVLRLNRNRLNSIPAKTFQLSN-LQHLDLSRNRVRRIEGLTFHGLHRLRSLKMQRNGITRLMDGAFWGLSNMEILQLEYNNLTEVSKGWLYGLLTLQQLHLAHNAISRIKPDAWEFCQKLAELDLNSNQLAR 310          

HSP 2 Score: 54.6842 bits (130), Expect = 1.983e-7
Identity = 52/194 (26.80%), Postives = 97/194 (50.00%), Query Frame = 1
Query:  349 LMPQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFS--FXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            LM   F  +S +E L ++   +  +   +L   ++L+ LH++   I  +    +  C     + L+SN+++ + + +F   + +  L +G+NR++ I    F     L  LDL  N I+  IED +  F  L  ++ L L  N +  +  +   GL+ L+ L LS N+I +I+ +AF  +K L+ + L  + LL
Sbjct:  239 LMDGAFWGLSNMEILQLEYNNLTEVSKGWLYGLLTLQQLHLAHNAISRIKPDAWEFCQKLAELDLNSNQLARLEEGSFMGLSVLEQLSIGHNRVSFIADGAFRGLSLLNTLDLKYNEISWTIEDMNGPFSALNNLRKLFLQGNRIRSVTKKSFTGLDLLEQLDLSNNAIMSIQGNAFSQMKKLEELLLNTSSLL 432          

HSP 3 Score: 49.2914 bits (116), Expect = 7.736e-6
Identity = 40/128 (31.25%), Postives = 64/128 (50.00%), Query Frame = 1
Query:  538 LSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            L++N+IS I+ +       +  LDLGNN L  +R   F     L+NL L+ NRI+ +E     NL    ++       +   P   F L +LQ+L LS+N +  IE   F  L  L+ + +++N + R
Sbjct:  112 LANNKISKISSERLRPLVALETLDLGNNNLQEVRTGAFPP-LPLKNLYLNNNRISVLEHGCLNNLSSTLLVLRLNRNRLNSIPAKTFQLSNLQHLDLSRNRVRRIEGLTFHGLHRLRSLKMQRNGITR 238          
BLAST of SMED30003270 vs. Ensembl Cavefish
Match: lrrtm4l1 (leucine-rich repeat transmembrane neuronal protein 4-like [Source:NCBI gene;Acc:103034268])

HSP 1 Score: 65.4698 bits (158), Expect = 5.369e-11
Identity = 54/187 (28.88%), Postives = 91/187 (48.66%), Query Frame = 1
Query:  358 QMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            + F+ + RL+ L++    I  + N       +L+ L +S  +++ L  G F      + + L SN ++ I  + F  C  +  LDLG NRL ++ R  F     L  L L  N+ +RI  F F  L  ++ L L  N +  +   + +   +LQ L LS N I++++   F  L +L+ +NLE N L
Sbjct:  100 RAFQGIRRLKELILSTNRISQLHNATFHGVPNLRSLDLSYNKLQELQPGQFHGLRKLQNLHLRSNGLTTIPIRAFIECRSLEFLDLGYNRLRILTRTAFLGLSRLMELHLEHNQFSRINLFLFPRLANLRGLYLQWNRIRAVNQGLPWIWYTLQKLDLSGNEIQSLDPVVFQCLPNLQVLNLESNKL 286          
BLAST of SMED30003270 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005797.1 (pep scaffold:Pmarinus_7.0:GL476660:214761:544110:-1 gene:ENSPMAG00000005255.1 transcript:ENSPMAT00000005797.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 67.3958 bits (163), Expect = 1.715e-13
Identity = 49/158 (31.01%), Postives = 87/158 (55.06%), Query Frame = 1
Query:  439 NFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNP 912
            N  +L+ LH+ + ++  L  G+F+     +++ L  N++S + +  FD    +  LDL  N+L  +  L+F+   +L+ L L  N++T + +  F  L ++K L L+ N L  +   +   L +LQ L+L +N +++I   AF NLKSL +I L  NP
Sbjct:   23 NLANLEKLHLYDNQLTSLPAGVFNRLVNLQKLHLYQNQMSALPNGVFDKLTELTILDLRTNQLQALPTLVFDSLGNLEVLGLCCNKLTDLPNGVFDKLTRLKQLGLDRNQLTSVPAGVFDRLGNLQKLWLHRNQLKSIPRGAFDNLKSLTHIWLFGNP 180          
BLAST of SMED30003270 vs. Ensembl Sea Lamprey
Match: lrrn1 (leucine rich repeat neuronal 1 [Source:ZFIN;Acc:ZDB-GENE-071126-1])

HSP 1 Score: 70.4774 bits (171), Expect = 2.370e-13
Identity = 53/167 (31.74%), Postives = 83/167 (49.70%), Query Frame = 1
Query:  415 TIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            ++ +  L N   L  LH+ E R+  L+ G  S  P  + + L+ NRIS +A   F     ++ L L +NRL  I R  F     L+ L +  N I  +   +F+ L  ++ L L    L  +  E L GLE L+ L L  N +E +   A  +L +LK+++L KNP+
Sbjct:   84 SVADVGLLNVSRLLTLHLEENRVTELADGCLSILPSLQELYLNHNRISSVAPDAFAGLGGLLRLHLNSNRLRAIDRRWFEATPHLEVLMIGENPIVGVAPANFRPLANLRSLVLAGTGLAHVPGEALLGLEKLESLSLYDNLLEAVPRAALRHLPALKFLDLNKNPV 250          
BLAST of SMED30003270 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000079.1 (pep scaffold:Pmarinus_7.0:GL476403:206014:206980:-1 gene:ENSPMAG00000000071.1 transcript:ENSPMAT00000000079.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 67.3958 bits (163), Expect = 6.010e-13
Identity = 58/194 (29.90%), Postives = 97/194 (50.00%), Query Frame = 1
Query:  352 MPQMFKLVSRLETLVVQAPIQTIENQFLKNFVS-----LKILHISETRIEHLSRGMFSDCPITERIRLSSNRISV--IADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNP 912
            +P M + +S +E L +       +NQ L+ F +     L+ LH+ E  IE +   +    P  E++ L+ N +S   I   TF    R+  L L  N L+ + R +      L+ L L  NRI+RI D  F  L  +  L L+ N + R+ P + +GL+ LQ + LS N + ++ + +F  L+SL +++   NP
Sbjct:   55 LPAMLQALSSVEVLYL------YDNQ-LEEFPAHLPPNLRELHLQENNIEVVPGAVLERTPFLEKLHLNDNSVSSAGIEPATFHGTPRLKLLFLSRNHLSSVPRGL---PRGLEELRLDDNRISRIPDRIFGQLTYLSRLALDDNQISRLPPGVFYGLKQLQRIDLSGNGLRSLSQGSFNGLESLSHLSACNNP 238          
BLAST of SMED30003270 vs. Ensembl Sea Lamprey
Match: lrig3 (leucine-rich repeats and immunoglobulin-like domains 3 [Source:NCBI gene;Acc:564554])

HSP 1 Score: 69.3218 bits (168), Expect = 6.585e-13
Identity = 53/156 (33.97%), Postives = 90/156 (57.69%), Query Frame = 1
Query:  463 HISETRIEHLSRGMFSDCPITERI-RLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLRPN 927
            +++  RI+ L    F     T +I +LS+NRI+V+  K F     + +LDL  NRL  +  L F+E  SL+ L L RNRI+ + D +F  L +++ + L  N L  +    L+GL+SL++L +S+N+I  ++ +A+   ++L  ++L  N L R N
Sbjct:    1 YLNNNRIKVLRPASFDTFGATLKILKLSNNRITVLTTKLFQ-LPSLQHLDLSRNRLQRVEGLAFSELASLEYLRLQRNRISSLMDGAFWGLHRIQKIQLQHNRLTEVSTGWLYGLQSLRHLHVSRNAITAMDSEAWKFCENLWELDLSHNGLTRLN 155          

HSP 2 Score: 59.6918 bits (143), Expect = 6.516e-10
Identity = 52/195 (26.67%), Postives = 89/195 (45.64%), Query Frame = 1
Query:  340 LIVLMPQMFKLVSRLETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEED---AFVNLKSLKYINLEKNPL 915
            + VL  ++F+L S     + +  +Q +E        SL+ L +   RI  L  G F      ++I+L  NR++ ++         + +L +  N +T +    +    +L  LDLS N +TR+ + +F  L  ++ L+L  N +  I      GL SL+ L L  N I    ED   AF  L +L  ++L  N +
Sbjct:   32 ITVLTTKLFQLPSLQHLDLSRNRLQRVEGLAFSELASLEYLRLQRNRISSLMDGAFWGLHRIQKIQLQHNRLTEVSTGWLYGLQSLRHLHVSRNAITAMDSEAWKFCENLWELDLSHNGLTRLNEAAFVELGALQSLSLAHNAISHIVEGAFRGLSSLRILDLGGNQISWTVEDMQGAFTGLSALSKLSLHGNQI 226          
BLAST of SMED30003270 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004666.1 (pep scaffold:Pmarinus_7.0:GL476584:91022:304495:-1 gene:ENSPMAG00000004069.1 transcript:ENSPMAT00000004666.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 61.6178 bits (148), Expect = 3.688e-11
Identity = 52/168 (30.95%), Postives = 80/168 (47.62%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNP 912
            I  +E     +   L  L +   ++  L  G+F      +++ L  NR+S I    FD    +  L L +N+LT +   +FN   +L+ L L  N++T     +F  L  +K L L+ N L  I    L    +LQ+L L  N +++I   AF NLKSL +I L  NP
Sbjct:   41 ITKLEPGVFDSLTQLTYLTLRNNQLTALPEGVFDRLVNLQQLYLHLNRLSSIPAGMFDKLTNLKELKLYSNQLTALPEGLFNRLGNLEALGLCCNKLTEQPSRAFDKLTWLKYLGLDGNQLKSIPAGCLTAWGNLQWLSLHVNQLKSIPRGAFDNLKSLTHIWLYNNP 208          

HSP 2 Score: 48.1358 bits (113), Expect = 1.111e-6
Identity = 49/149 (32.89%), Postives = 76/149 (51.01%), Query Frame = 1
Query:  472 ETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFG-LESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            E R+  +  G+ +    T+ + L +N+I+ +    FDS  ++  L L NN+LT +   +F+   +LQ L L  NR++ I    F  L  +K L L +N L  + PE LF  L +L+ L L  N +      AF  L  LKY+ L+ N L
Sbjct:   17 ERRLASVPAGIPT---TTQVLYLYTNKITKLEPGVFDSLTQLTYLTLRNNQLTALPEGVFDRLVNLQQLYLHLNRLSSIPAGMFDKLTNLKELKLYSNQLTAL-PEGLFNRLGNLEALGLCCNKLTEQPSRAFDKLTWLKYLGLDGNQL 161          
BLAST of SMED30003270 vs. Ensembl Nematostella
Match: EDO49136 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGZ6])

HSP 1 Score: 69.707 bits (169), Expect = 6.587e-13
Identity = 59/228 (25.88%), Postives = 110/228 (48.25%), Query Frame = 1
Query:  415 TIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKN-SIETIEEDAF--VNLKSLKYINLEKNPLLRPNMXXXXXXXXXXXXXXGVKCVDEQYSDYSKVSINVSNEFEKYPICSGIRKRNETG 1089
            T+   +L+N   L  L++   +I++++ G F+D    E + LS N+++ I++  F     +  L L  N +  +   +F    +L+ L ++RN IT++    F+N   ++ L ++ N L  IKP    G  +L+ L L+ N +++T+E+D F   NL +L+ + +    L+R  M                     Q+ D+ KV + +S    KYPI +    R + G
Sbjct:   65 TVTRGYLRNLRQLLELNLGMNQIDYIASGTFTDMAKLELLDLSGNQLTFISEGMFTGLRNLTTLFLFMNEILDLPASLFWGTPNLKILSINRNNITKLHQHLFRNSLHLEYLFMDDNNLTTIKPNTFKGARALEILSLTNNVALKTVEDDTFNVANLPNLRLMYILNTQLVRIRM--------------------SQFKDHQKVGVVISPTVAKYPIMAANDSRLQGG 272          
BLAST of SMED30003270 vs. Ensembl Nematostella
Match: EDO48179 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RK32])

HSP 1 Score: 60.8474 bits (146), Expect = 9.053e-10
Identity = 51/162 (31.48%), Postives = 88/162 (54.32%), Query Frame = 1
Query:  451 LKILHISETRIEHLSRGMFSDC---PITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDFS--FXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            L+ L++   +I  +    F+D    P+  ++ L  NR+S I D TF     +  L+L NNR+  I +  F++  SL+ LDLS N I+  +E+ +  F+ L  +  L L+ N +  I      GLE+++YL LS N I +I+E++F  +  L+ + L  + L+
Sbjct:  321 LQNLYLQSNQISTIITTKFADWRSFPVLRKLNLERNRLSHIQDTTFKHLLALKVLNLANNRIYHISQGSFSDLRSLEGLDLSNNDISWTVEEMNGPFRGLTNLASLRLDGNRITAIAATAFLGLENIKYLNLSANIITSIQENSFQGMDKLQKLWLNTSQLM 482          

HSP 2 Score: 55.4546 bits (132), Expect = 3.961e-8
Identity = 55/173 (31.79%), Postives = 81/173 (46.82%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILH-ISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKS---LKYINLEKNPL 915
            I +I +    N  +L+ L+ I+  R   L+     DC      +L  NR++ +          +  LDL  N  T I    FN   SL+ L LS+NRI+ I   +F    K++ L L+ N    I+    FGL  LQ L+L  N I TI    F + +S   L+ +NLE+N L
Sbjct:  191 ISSIGHHAFTNLSNLQNLYSIAYLRYTSLTEYKKEDCIK----KLGKNRLTSVPSDALSQLQSLKRLDLSRNFFTSILASAFNRLSSLEVLKLSKNRISTIRG-AFWGQNKLQQLYLDRNNFTAIQTSSFFGLRDLQNLYLQSNQISTIITTKFADWRSFPVLRKLNLERNRL 358          
BLAST of SMED30003270 vs. Ensembl Nematostella
Match: EDO39412 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SA74])

HSP 1 Score: 56.6102 bits (135), Expect = 1.429e-8
Identity = 43/184 (23.37%), Postives = 95/184 (51.63%), Query Frame = 1
Query:  367 KLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            +++ RL  L + +  I  I N FL  F +L+ L++S  ++E L   +F++    + + +++N +  +   +F +  R+++L L NN+L  +   + +    L++L ++ N++  +++ +F     ++++    NL+  +   +  GL SLQ +   +N +  +    F N  SLK + L  N L
Sbjct:  174 RVMDRLSYLWLSSTGITEIPNGFLDYFPALEALYLSNNKLEQLPSALFNNNTRLQYLYINNNELEHLHSSSFSNNARLLHLSLVNNKLVRLPLYLLSNNTKLESLSIAYNQLEYLQNDTFFYNADLQIIIFENNLITSLPENLTDGLNSLQIISFPRNKLSKVPSKIFQNKPSLKIVFLGMNNL 357          

HSP 2 Score: 51.9878 bits (123), Expect = 4.014e-7
Identity = 43/164 (26.22%), Postives = 74/164 (45.12%), Query Frame = 1
Query:  433 LKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLES-LQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            LK    L IL++S   ++ +  G F D    +   +  N+I  +AD  F + +    LD+G N++  +   +      L  L LS   IT I +  F +          +N  +   P  LF   + LQYL+++ N +E +   +F N   L +++L  N L+R
Sbjct:  101 LKGLRRLTILYLSNNFLDSIPGGCFLDLKSLKHFFVGDNQIKKVADDVFQNVSNGFKLDIGRNKMNGVPDAVKRVMDRLSYLWLSSTGITEIPN-GFLDYFPALEALYLSNNKLEQLPSALFNNNTRLQYLYINNNELEHLHSSSFSNNARLLHLSLVNNKLVR 263          
BLAST of SMED30003270 vs. Ensembl Nematostella
Match: EDO35407 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLJ8])

HSP 1 Score: 52.373 bits (124), Expect = 1.208e-7
Identity = 49/177 (27.68%), Postives = 89/177 (50.28%), Query Frame = 1
Query:  394 VVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDL--SRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEI---LFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            V ++P++ ++N   +++  LK L +S   I  +  G F +    +++ L  N+I  ++   F     +  LDL  N L  + R +F    SL+ L L  +RN  +RIE+ +F  L  ++ L+++A  + +I       L  LE L  L ++ NS+ T+   A   +K LK ++L  N
Sbjct:    1 VSRSPLKLLKNTSFQDYQYLKWLDLSNNDISDIQDGTFENLRYLKQLNLGENKIRTVSASMFKGLVSLEKLDLRFNDLKALPRGVFKPLKSLRELFLHDNRNMGSRIEEGAFNGLDNLRKLHVSATGMKKITQGTFGSLMSLEFLDVLHINNNSLTTVPLHALRKVKFLKELDLSAN 177          
BLAST of SMED30003270 vs. Ensembl Nematostella
Match: EDO31669 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXA1])

HSP 1 Score: 51.9878 bits (123), Expect = 4.146e-7
Identity = 43/140 (30.71%), Postives = 76/140 (54.29%), Query Frame = 1
Query:  448 SLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAF 867
             +K L++    I+ +  G F +    E + LS N++  +    FD+ + +  L L +NRLT + R +F+   SL+ L L RN+I  IE ++F +LK +  L L++N L  I  + L GL +++ + +  N +  +  DAF
Sbjct:  120 GMKKLNLRRNGIKEIEFGAFRNLTALESLILSKNKLRRLTYAMFDTLSYLRLLSLVDNRLTGLTRSMFSGLKSLRELYLQRNQIQDIEPWTFTSLKSLASLYLDSNQLTVISRDTLLGLTNVKDIHVYFNKLRYVARDAF 259          

HSP 2 Score: 49.6766 bits (117), Expect = 2.215e-6
Identity = 49/157 (31.21%), Postives = 74/157 (47.13%), Query Frame = 1
Query:  517 PITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMR------------------------IKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            P T  + L+ NR+S++    F   N +  LDL  NR + IR   F     ++ L+L RN I  IE  +F+NL  ++ L L+ N L R                        +   +  GL+SL+ L+L +N I+ IE   F +LKSL  + L+ N L
Sbjct:   71 PRTSTLLLNDNRLSLLRYDFFLGLNNIRTLDLSGNRFSKIRFNTFGYLPGMKKLNLRRNGIKEIEFGAFRNLTALESLILSKNKLRRLTYAMFDTLSYLRLLSLVDNRLTGLTRSMFSGLKSLRELYLQRNQIQDIEPWTFTSLKSLASLYLDSNQL 227          
BLAST of SMED30003270 vs. Ensembl Medaka
Match: igfals (insulin like growth factor binding protein acid labile subunit [Source:NCBI gene;Acc:101172904])

HSP 1 Score: 82.4185 bits (202), Expect = 2.291e-16
Identity = 58/167 (34.73%), Postives = 96/167 (57.49%), Query Frame = 1
Query:  415 TIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            T++ Q  K   SL  +H+   R+  L   +F + P    + L +N+IS I ++ F   + +  L+LG N LTV+   +F++ HSL+ L L+ NR+  ++   F+NL ++K L+L  N +  IK  +   L  LQ L+L++N I TI   AFV +KSL++++L  N L
Sbjct:  106 TLDPQAFKGLRSLAHVHLERNRLRALPGTIFQNTPNLASLSLHNNQISRIDERLFAGLSHMWLLNLGWNSLTVLPETVFHDLHSLRELILAGNRLAYLQPQLFQNLVELKELDLTGNHVKVIKANVFVKLTKLQKLYLARNQIVTIVPRAFVGMKSLRWLDLTNNKL 272          

HSP 2 Score: 59.3066 bits (142), Expect = 4.477e-9
Identity = 58/211 (27.49%), Postives = 100/211 (47.39%), Query Frame = 1
Query:  271 RFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINL 900
            R +  L A    +  LL    +SL VL   +F  +  L  L++    +  ++ Q  +N V LK L ++   ++ +   +F      +++ L+ N+I  I  + F     +  LDL NN+LT +    F   HSL  L LS N +T I   +F++L+ ++ L L+ N +  +   I  GL  L+ L L  N ++  +  +F  L  +  INL
Sbjct:  154 RIDERLFAGLSHM-WLLNLGWNSLTVLPETVFHDLHSLRELILAGNRLAYLQPQLFQNLVELKELDLTGNHVKVIKANVFVKLTKLQKLYLARNQIVTIVPRAFVGMKSLRWLDLTNNKLTSLHEDTFLGLHSLHVLRLSNNSVTGIRPRTFRDLQYLEELQLSHNKIRVLGERIFDGLGHLEVLELQHNQVQEAQMGSFTGLSHVAVINL 363          

HSP 3 Score: 49.2914 bits (116), Expect = 7.146e-6
Identity = 45/185 (24.32%), Postives = 86/185 (46.49%), Query Frame = 1
Query:  319 LITTESSLIVLMPQMFKLVSRLETL-VVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFV 870
            L    + ++ ++P+ F  +  L  L +    + ++         SL +L +S   +  +    F D    E ++LS N+I V+ ++ FD    +  L+L +N++   +   F     +  ++LS +  + + D  FK L K+  L+L+   L RI  +   GL  L+ LFL  N+I  +E  +F 
Sbjct:  241 LYLARNQIVTIVPRAFVGMKSLRWLDLTNNKLTSLHEDTFLGLHSLHVLRLSNNSVTGIRPRTFRDLQYLEELQLSHNKIRVLGERIFDGLGHLEVLELQHNQVQEAQMGSFTGLSHVAVINLSGSCFSSLPDQMFKGLSKLHSLHLDRGCLTRIPAQAFVGLSGLRRLFLQHNNISVVEYRSFA 425          
BLAST of SMED30003270 vs. Ensembl Medaka
Match: lrrn3b (leucine rich repeat neuronal 3 [Source:NCBI gene;Acc:101158247])

HSP 1 Score: 69.3218 bits (168), Expect = 4.223e-12
Identity = 51/174 (29.31%), Postives = 88/174 (50.57%), Query Frame = 1
Query:  400 QAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            Q  + +I +  L N   L  LH+ E  ++ L     S+    + + ++ N IS I+   F   + +V L L +N+LTVI+R  F    +L+ L +  N +  I+D +FK L  ++ L L    L  +  + L GL+ L+ +    N    +   A  N+K+LK+++L KNP+ R
Sbjct:  101 QNNLSSISDIHLGNLSQLISLHMEENWLQRLPEQCLSNVANLQELYMNHNLISYISPMAFQGMSNLVRLHLNSNKLTVIQREWFEPMPNLEILMIGENPVLSIDDMNFKPLSNLRSLVLTRMNLSHLPEKALTGLDKLESISFYDNIFPEVPHSALKNVKNLKFLDLNKNPIAR 274          
BLAST of SMED30003270 vs. Ensembl Medaka
Match: lrig1 (leucine rich repeats and immunoglobulin like domains 1 [Source:NCBI gene;Acc:101171324])

HSP 1 Score: 69.3218 bits (168), Expect = 4.409e-12
Identity = 86/293 (29.35%), Postives = 132/293 (45.05%), Query Frame = 1
Query:  124 EEPCQKKCRCKHKE-KSSKIEVAHCTVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQAP-IQTIENQFLKNFVSLKILHISETRIEHLSRGMF-----------SDCPIT--------------ERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLR 921
            E PC + C C       S++E+    +D     VS N      L   K    N +  A+   +  L +  + + +  +P +    SR+ +L +    I++I+ + ++  VS++ L +S   I  L    F           S+  I+              + +RLS NRIS I  + F    R+  L+L  NR+  I  L F    SL+ L L RN I+++ D +F +L KMKVL+L  N L  +    L+GL SL  LFLS NSI  I  D     + L+ +NL  N L R
Sbjct:   33 ELPCAQNCTCDGDSVDCSRLELTATPLDLPARTVSLN------LGHNKLTSINPEAFASLPNLRELHL--DHNELTSIPDLGHFASRIVSLYLHHNNIRSIDGRRIRELVSVETLDLSNNEITELRGHCFPAGLQIRDLYLSNNKISVLELGALDRLGSSLQVLRLSRNRISQIPIRAF-QLPRLTQLELNRNRIRQIEGLTFQGLSSLEVLKLQRNSISKLTDGAFWDLAKMKVLHLEYNNLTEVNSGSLYGLTSLTQLFLSNNSIARINPDGLKFCQRLRELNLSYNNLTR 316          

HSP 2 Score: 49.2914 bits (116), Expect = 9.501e-6
Identity = 54/213 (25.35%), Postives = 96/213 (45.07%), Query Frame = 1
Query:  364 FKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDL------------------------GNNRLTVIRRLIFNEGHSLQNLDLSRNRIT-RIEDF--SFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLL 918
            F+ +S LE L +Q   I  + +    +   +K+LH+    +  ++ G         ++ LS+N I+ I       C R+  L+L                        G+N ++ I    F    +++ L+L  N I+  IED   +F  L ++  L L  N +  +  E   GLESL++L L +N I +I+ DAF  +++LK + ++ N  L
Sbjct:  226 FQGLSSLEVLKLQRNSISKLTDGAFWDLAKMKVLHLEYNNLTEVNSGSLYGLTSLTQLFLSNNSIARINPDGLKFCQRLRELNLSYNNLTRLDEGSLAMLGDLHTLRLGHNSISQINEGAFRGLKAVRILELDHNDISGTIEDTNGAFSGLDRLNKLTLFGNKIKSVAKEAFSGLESLEHLNLGENPIRSIQPDAFSKMRNLKNLIMDSNSFL 438          
BLAST of SMED30003270 vs. Ensembl Medaka
Match: ENSORLT00000000072.2 (pep primary_assembly:ASM223467v1:1:1576629:1604541:-1 gene:ENSORLG00000000055.2 transcript:ENSORLT00000000072.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 68.1662 bits (165), Expect = 5.080e-12
Identity = 46/169 (27.22%), Postives = 77/169 (45.56%), Query Frame = 1
Query:  409 IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            +  I    L     L++LH+ E +I ++ RG F +    ER+RL+ NR+S + +  F     +  LDL  NR+  + R  F     L+NL L +N I+ +E+ +F+                         L SL+ L L+ N+I +I   +F ++  L+   L  N L
Sbjct:   84 LTVITKSDLSGLRHLRVLHLMENQISNIERGAFDELKELERLRLNRNRLSQLPELLFQKNEALSRLDLSENRIQAVPRRAFRGAADLKNLQLDKNHISCVEEGAFR------------------------ALRSLEVLTLNNNNISSIPVSSFNHMPKLRTFRLHSNSL 228          

HSP 2 Score: 55.4546 bits (132), Expect = 5.473e-8
Identity = 40/129 (31.01%), Postives = 68/129 (52.71%), Query Frame = 1
Query:  523 TERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            TER+ L+ N ++VI          +  L L  N+++ I R  F+E   L+ L L+RNR++++ +  F+  + +  L+L+ N +  +      G   L+ L L KN I  +EE AF  L+SL+ + L  N
Sbjct:   74 TERLDLNGNNLTVITKSDLSGLRHLRVLHLMENQISNIERGAFDELKELERLRLNRNRLSQLPELLFQKNEALSRLDLSENRIQAVPRRAFRGAADLKNLQLDKNHISCVEEGAFRALRSLEVLTLNNN 202          
BLAST of SMED30003270 vs. Ensembl Medaka
Match: lrrtm4l1 (leucine-rich repeat transmembrane neuronal protein 4-like [Source:NCBI gene;Acc:101154965])

HSP 1 Score: 67.781 bits (164), Expect = 1.131e-11
Identity = 58/187 (31.02%), Postives = 92/187 (49.20%), Query Frame = 1
Query:  358 QMFKLVSRLETLVVQA-PIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPL 915
            + F+ V RL+ L++ +  I ++ N       +L+ L +S  ++E L  G F      + + L SN +S I  + F  C  +  LDLG NR+  + R  F     L  L L  N+ TRI  F F  L  ++ L L  N +  +   + +   +LQ L LS N I+T++   F  L +L+ +NLE N L
Sbjct:  121 RAFQGVRRLKELILSSNRITSLHNSTFHGIPNLRSLDLSFNKLEILQPGQFHGLRKLQNLHLRSNGLSNIPIRVFLECRSLEFLDLGYNRIKALTRTTFLGLQRLMELHLEHNQFTRINFFLFPRLANLRSLYLQWNRIRVVNQGLPWIWYTLQKLDLSGNEIQTLDPAVFHCLPNLQVLNLESNKL 307          
BLAST of SMED30003270 vs. Planmine SMEST
Match: SMESG000052792.1 (SMESG000052792.1)

HSP 1 Score: 756.133 bits (1951), Expect = 0.000e+0
Identity = 418/419 (99.76%), Postives = 418/419 (99.76%), Query Frame = 1
Query:   85 NYSAQTYFEGGLIEEPCQKKCRCKHKEKSSKIEVAHCTVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLRPNMXXXXXXXXXXXXXXGVKCVDEQYSDYSKVSINVSNEFEKYPICSGIRKRNETGENKPLIQNSESKNPQPNPTKVWKTSVGPSIFDLHPYLFPMIFGGXXXXXXXXXXXXXXCHIKNQQINELRLQELQNLPMMQSTL 1341
            NYSAQTYFEGGLIEEPCQKKCRCKHKEKSSKIEVAHCTVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFKNLKKMKVLNLNANLLMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLRPNMVLFLNLDNLTVLLTGVKCVDEQYSDYSKVSINVSNEFEKYPICSGIRKRNETGENKPLIQNSESKNPQPNPTKVWKTSVGPSIFDLHPYLFPMIFGGLSVLLISIIVIA VCHIKNQQINELRLQELQNLPMMQSTL
Sbjct:   16 NYSAQTYFEGGLIEEPCQKKCRCKHKEKSSKIEVAHCTVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESSLIVLMPQMFKLVSRLETLVVQAPIQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFKNLKKMKVLNLNANLLMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLRPNMVLFLNLDNLTVLLTGVKCVDEQYSDYSKVSINVSNEFEKYPICSGIRKRNETGENKPLIQNSESKNPQPNPTKVWKTSVGPSIFDLHPYLFPMIFGGLSVLLISIIVIAFVCHIKNQQINELRLQELQNLPMMQSTL 434          
BLAST of SMED30003270 vs. Planmine SMEST
Match: SMESG000045574.1 (SMESG000045574.1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.693e-10
Identity = 68/290 (23.45%), Postives = 128/290 (44.14%), Query Frame = 1
Query:  133 CQKKCRCKHKEKSSKIEVAHCTVDEKDTFVSCNVEWPILLMTVK-KLRFNAD---LLAACQRISGLLITTESSLIVLMPQMFKLVSR-LETLVVQAP--IQTIENQFLKNFVSLKILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCN--RVVNLD----------------------LGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKN 909
            C   C+C          V  C+V +    + C V W I  + V   + + +D   L   C   +   I      I  +P  + +  R LE L++  P  I  ++    +N + LK   ++  ++  ++  +F+ C   + + L++N I VI +  F +C   R +NL                       L +NR++ I+   F+    + +++L+ N++  ++  SF N+  M  L LN N +  I  +   GL++LQYL L  N +  +EE  F  +K L+ + L+++
Sbjct:    7 CPNHCQCD--------GVVKCSVVQSHQSIFCPVNWEITSIIVSITMPYKSDWNNLFLNCSTSNITAIMINPGNIRSVPASYFVDFRNLEKLIINGPGIIDRLDEGIFRNRIHLKKFKMTSNKLTIITEKLFNSCSTLQELSLANNMIKVIFELAFHTCKNLRFINLSHNLLLGIYEKTFEGLVNLYVLILAHNRISFIKPFAFSSLRKIHSIELNHNQLLSLDIRSFFNIHSMIRLLLNDNQIRDISMKAFKGLDNLQYLSLKNNKLMLLEESMFSEMKRLETVELDED 288          
BLAST of SMED30003270 vs. Planmine SMEST
Match: SMESG000017864.1 (SMESG000017864.1)

HSP 1 Score: 51.6026 bits (122), Expect = 1.317e-6
Identity = 48/157 (30.57%), Postives = 76/157 (48.41%), Query Frame = 1
Query:  454 KILHISETRIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGL-ESLQYLFLSKNSIETIEEDAFVNLKSLK--YINLEKNPL 915
            + L I +T    LS G+FS       + +S N I  + +  FD    +  L L  N + ++    F     LQ LD++ N IT IE F+F NL  +  + L +  L  I      G+ E+L  + +S N I +I +D+FV + +L    IN+ KN +
Sbjct: 1398 RTLTIVQTHSLELSSGIFSQFIYLGYLNISDNSILELYNGLFDGLFNLRYLILSKNYIRILPSYAFQFLKQLQILDITDNPITTIESFAFHNLISITHIELPSMKLYEINDYTFLGMSENLISINMSNNDIRSISQDSFVGVNNLSTLVINVNKNDI 1554          
BLAST of SMED30003270 vs. Planmine SMEST
Match: SMESG000047911.1 (SMESG000047911.1)

HSP 1 Score: 50.8322 bits (120), Expect = 1.664e-6
Identity = 44/149 (29.53%), Postives = 68/149 (45.64%), Query Frame = 1
Query:  442 FVSLKILHISET-RIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSL 885
            F   K+ H++ T +I++L+   F      ER+ L  N ++VI+  TF    +V  ++L  N +  +    F     L +LDLS N ++ I D                        E+   + +LQ L LS N+I  IE +AFV LKSL
Sbjct:  253 FAVKKLKHLNMTMKIKNLTALTFYGLTDLERLELQGNHLTVISPGTFQQVQKVTIINLSRNAIRCLLPETFRNLPKLLSLDLSYNDLSYISD------------------------EVFSEISTLQTLDLSDNNIYVIERNAFVGLKSL 377          
BLAST of SMED30003270 vs. Planmine SMEST
Match: SMESG000045664.1 (SMESG000045664.1)

HSP 1 Score: 50.8322 bits (120), Expect = 1.664e-6
Identity = 44/149 (29.53%), Postives = 68/149 (45.64%), Query Frame = 1
Query:  442 FVSLKILHISET-RIEHLSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIRRLIFNEGHSLQNLDLSRNRITRIEDFSFXXXXXXXXXXXXXXXXMRIKPEILFGLESLQYLFLSKNSIETIEEDAFVNLKSL 885
            F   K+ H++ T +I++L+   F      ER+ L  N ++VI+  TF    +V  ++L  N +  +    F     L +LDLS N ++ I D                        E+   + +LQ L LS N+I  IE +AFV LKSL
Sbjct:  253 FAVKKLKHLNMTMKIKNLTALTFYGLTDLERLELQGNHLTVISPGTFQQVQKVTIINLSRNAIRCLLPETFRNLPKLLSLDLSYNDLSYISD------------------------EVFSEISTLQTLDLSDNNIYVIERNAFVGLKSL 377          
The following BLAST results are available for this feature:
BLAST of SMED30003270 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
IGFALS3.908e-1631.18insulin like growth factor binding protein acid la... [more]
IGFALS4.530e-1631.18insulin like growth factor binding protein acid la... [more]
LRIG32.419e-1130.41leucine rich repeats and immunoglobulin like domai... [more]
LRIG32.832e-1130.41leucine rich repeats and immunoglobulin like domai... [more]
LRIG13.352e-1128.90leucine rich repeats and immunoglobulin like domai... [more]
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BLAST of SMED30003270 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sma-101.991e-1127.54Leucine-rich repeats and immunoglobulin-like domai... [more]
sma-101.991e-1127.54Leucine-rich repeats and immunoglobulin-like domai... [more]
sma-102.194e-1127.54Leucine-rich repeats and immunoglobulin-like domai... [more]
sma-102.194e-1127.54Leucine-rich repeats and immunoglobulin-like domai... [more]
lron-21.500e-1027.50ELRR (Extracellular Leucine-Rich Repeat) ONly [So... [more]
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BLAST of SMED30003270 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sli2.842e-1225.82gene:FBgn0264089 transcript:FBtr0330733[more]
sli2.842e-1225.82gene:FBgn0264089 transcript:FBtr0330731[more]
sli3.102e-1225.82gene:FBgn0264089 transcript:FBtr0330734[more]
CG78963.025e-1126.94gene:FBgn0039728 transcript:FBtr0085545[more]
CG320551.452e-931.47gene:FBgn0052055 transcript:FBtr0076304[more]
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BLAST of SMED30003270 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lrig11.862e-1135.00leucine-rich repeats and immunoglobulin-like domai... [more]
lrig11.943e-1135.00leucine-rich repeats and immunoglobulin-like domai... [more]
lrig12.067e-1135.00leucine-rich repeats and immunoglobulin-like domai... [more]
lrrtm4l13.736e-1129.41leucine rich repeat transmembrane neuronal 4 like ... [more]
lrrn3a5.130e-1124.33leucine rich repeat neuronal 3a [Source:ZFIN;Acc:Z... [more]
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BLAST of SMED30003270 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
IGFALS1.272e-1127.51insulin like growth factor binding protein acid la... [more]
LRIG29.189e-1133.15leucine-rich repeats and immunoglobulin like domai... [more]
LRIG21.005e-1033.15leucine-rich repeats and immunoglobulin like domai... [more]
LRIG21.038e-1033.15leucine-rich repeats and immunoglobulin like domai... [more]
nprl22.084e-1024.87NPR2 like, GATOR1 complex subunit [Source:Xenbase;... [more]
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BLAST of SMED30003270 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Lrig13.986e-1231.28leucine-rich repeats and immunoglobulin-like domai... [more]
Lrig13.986e-1231.28leucine-rich repeats and immunoglobulin-like domai... [more]
Lrig13.986e-1231.28leucine-rich repeats and immunoglobulin-like domai... [more]
Lrig32.587e-1129.90leucine-rich repeats and immunoglobulin-like domai... [more]
Slit12.715e-1128.85slit guidance ligand 1 [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of SMED30003270 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P35858|ALS_HUMAN2.176e-1531.18Insulin-like growth factor-binding protein complex... [more]
sp|O02833|ALS_PAPHA1.414e-1430.65Insulin-like growth factor-binding protein complex... [more]
sp|P70193|LRIG1_MOUSE2.788e-1131.28Leucine-rich repeats and immunoglobulin-like domai... [more]
sp|P24014|SLIT_DROME2.849e-1125.82Protein slit OS=Drosophila melanogaster OX=7227 GN... [more]
sp|P35859|ALS_RAT7.181e-1128.96Insulin-like growth factor-binding protein complex... [more]
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BLAST of SMED30003270 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3Q1FV137.048e-1628.52Insulin-like growth factor binding protein, acid l... [more]
A0A3B3YX168.196e-1528.14Insulin-like growth factor binding protein, acid l... [more]
A0A087YST59.207e-1528.14Insulin-like growth factor binding protein, acid l... [more]
A0A3B3TLZ09.289e-1528.14Insulin-like growth factor binding protein, acid l... [more]
A0A1S3R9S01.561e-1429.48insulin-like growth factor-binding protein complex... [more]
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BLAST of SMED30003270 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
igfals8.090e-1727.92insulin like growth factor binding protein acid la... [more]
lrrn3a1.341e-1330.43leucine rich repeat neuronal 3 [Source:NCBI gene;A... [more]
ENSAMXT00000025596.22.841e-1328.43pep primary_assembly:Astyanax_mexicanus-2.0:1:1654... [more]
lrig31.244e-1236.71leucine rich repeats and immunoglobulin like domai... [more]
lrrtm4l15.369e-1128.88leucine-rich repeat transmembrane neuronal protein... [more]
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BLAST of SMED30003270 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000005797.11.715e-1331.01pep scaffold:Pmarinus_7.0:GL476660:214761:544110:-... [more]
lrrn12.370e-1331.74leucine rich repeat neuronal 1 [Source:ZFIN;Acc:ZD... [more]
ENSPMAT00000000079.16.010e-1329.90pep scaffold:Pmarinus_7.0:GL476403:206014:206980:-... [more]
lrig36.585e-1333.97leucine-rich repeats and immunoglobulin-like domai... [more]
ENSPMAT00000004666.13.688e-1130.95pep scaffold:Pmarinus_7.0:GL476584:91022:304495:-1... [more]
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BLAST of SMED30003270 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003270 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO491366.587e-1325.88Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO481799.053e-1031.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO394121.429e-823.37Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO354071.208e-727.68Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO316694.146e-730.71Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30003270 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
igfals2.291e-1634.73insulin like growth factor binding protein acid la... [more]
lrrn3b4.223e-1229.31leucine rich repeat neuronal 3 [Source:NCBI gene;A... [more]
lrig14.409e-1229.35leucine rich repeats and immunoglobulin like domai... [more]
ENSORLT00000000072.25.080e-1227.22pep primary_assembly:ASM223467v1:1:1576629:1604541... [more]
lrrtm4l11.131e-1131.02leucine-rich repeat transmembrane neuronal protein... [more]
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BLAST of SMED30003270 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000052792.10.000e+099.76SMESG000052792.1[more]
SMESG000045574.11.693e-1023.45SMESG000045574.1[more]
SMESG000017864.11.317e-630.57SMESG000017864.1[more]
SMESG000047911.11.664e-629.53SMESG000047911.1[more]
SMESG000045664.11.664e-629.53SMESG000045664.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003270 ID=SMED30003270|Name=SMED30003270|organism=Schmidtea mediterranea sexual|type=transcript|length=1428bp
TTGATACGAAACAGTATTAAATTATTCGGCAAAATTTAAATGAAGAAATT
GTTATATATTTTATTAGTAATAATTTTGACATTGAATTATTCGGCGCAAA
CTTATTTCGAAGGTGGCTTAATTGAGGAACCATGCCAGAAAAAGTGCCGA
TGTAAGCACAAAGAAAAATCCAGTAAAATTGAAGTGGCTCATTGCACTGT
TGATGAGAAAGACACCTTTGTGTCATGCAATGTTGAATGGCCGATATTGT
TAATGACGGTTAAGAAACTTCGTTTTAACGCCGATCTCCTTGCGGCTTGT
CAACGCATCAGTGGATTACTCATTACAACAGAAAGCTCTCTGATTGTTCT
AATGCCGCAGATGTTTAAATTAGTTTCTCGCCTCGAAACACTAGTTGTTC
AAGCTCCGATTCAAACAATTGAAAATCAGTTTCTGAAAAATTTTGTTTCC
TTGAAGATTTTGCACATTTCAGAAACGAGAATCGAGCATTTGTCAAGGGG
GATGTTTTCCGATTGCCCGATCACAGAGAGAATTCGCTTGAGTTCAAATC
GTATTTCAGTAATTGCAGATAAAACATTCGACAGTTGCAATCGAGTAGTT
AATTTGGATCTGGGAAACAACCGACTAACGGTCATCCGTCGTTTGATATT
CAATGAAGGACATAGTTTGCAAAATTTAGATCTAAGTCGCAACCGAATCA
CGAGAATTGAAGATTTCAGTTTTAAGAATCTGAAGAAAATGAAAGTTTTG
AACCTGAATGCGAATCTTTTGATGAGAATTAAACCTGAAATTTTATTCGG
CTTGGAGAGTCTTCAGTATTTGTTTTTGAGTAAGAATAGCATCGAAACGA
TTGAAGAGGATGCTTTTGTTAATTTAAAATCTCTGAAATATATCAACCTG
GAAAAGAATCCACTGCTTCGGCCGAATATGGTTTTATTCCTAAACCTCGA
CAATTTGACAGTGCTATTAACCGGAGTGAAATGTGTCGATGAGCAGTATT
CCGATTACTCGAAGGTCTCAATTAATGTTTCGAATGAGTTTGAGAAATAT
CCCATCTGTTCTGGAATCCGAAAACGAAACGAGACGGGAGAAAATAAGCC
TTTAATTCAAAATTCTGAATCGAAAAATCCACAACCAAATCCGACAAAGG
TTTGGAAAACATCCGTCGGCCCGTCCATTTTCGATCTTCATCCTTATTTG
TTCCCAATGATATTCGGAGGTCTATCGGTTCTACTCATCTCAATCATTGT
CATTGCTGTCGTTTGCCATATTAAAAATCAACAGATTAATGAGTTGAGAT
TGCAAGAATTACAGAATTTGCCCATGATGCAATCAACTTTGTAAGACTAT
TTGTATGAATGTATGAAATTGTAAATATCACAGATGACAAAAAAAAATTA
AAGTTTTTTTTACCTCGATATAGATATG
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protein sequence of SMED30003270-orf-1

>SMED30003270-orf-1 ID=SMED30003270-orf-1|Name=SMED30003270-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=435bp
MKKLLYILLVIILTLNYSAQTYFEGGLIEEPCQKKCRCKHKEKSSKIEVA
HCTVDEKDTFVSCNVEWPILLMTVKKLRFNADLLAACQRISGLLITTESS
LIVLMPQMFKLVSRLETLVVQAPIQTIENQFLKNFVSLKILHISETRIEH
LSRGMFSDCPITERIRLSSNRISVIADKTFDSCNRVVNLDLGNNRLTVIR
RLIFNEGHSLQNLDLSRNRITRIEDFSFKNLKKMKVLNLNANLLMRIKPE
ILFGLESLQYLFLSKNSIETIEEDAFVNLKSLKYINLEKNPLLRPNMVLF
LNLDNLTVLLTGVKCVDEQYSDYSKVSINVSNEFEKYPICSGIRKRNETG
ENKPLIQNSESKNPQPNPTKVWKTSVGPSIFDLHPYLFPMIFGGLSVLLI
SIIVIAVVCHIKNQQINELRLQELQNLPMMQSTL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0003104enteric muscle cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR003591Leu-rich_rpt_typical-subtyp
IPR001611Leu-rich_rpt
IPR032675LRR_dom_sf
IPR026906LRR_5
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005149interleukin-1 receptor binding
Vocabulary: biological process
TermDefinition
GO:0002224toll-like receptor signaling pathway
GO:0002237response to molecule of bacterial origin
GO:0007409axonogenesis
Vocabulary: cellular component
TermDefinition
GO:0005578proteinaceous extracellular matrix
GO:0005615extracellular space
GO:0005886plasma membrane
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003591Leucine-rich repeat, typical subtypeSMARTSM00369LRR_typ_2coord: 135..158
e-value: 12.0
score: 12.5
coord: 231..254
e-value: 9.0
score: 13.6
coord: 255..278
e-value: 0.023
score: 23.9
coord: 183..206
e-value: 390.0
score: 0.3
coord: 163..182
e-value: 250.0
score: 1.8
coord: 208..230
e-value: 11.0
score: 13.1
NoneNo IPR availableSMARTSM00365LRR_sd22_2coord: 255..276
e-value: 5.7
score: 15.9
coord: 183..204
e-value: 580.0
score: 1.2
coord: 207..228
e-value: 3.9
score: 16.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 352..369
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 350..369
NoneNo IPR availablePANTHERPTHR46473FAMILY NOT NAMEDcoord: 163..295
coord: 19..251
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.774
NoneNo IPR availableSUPERFAMILYSSF52058L domain-likecoord: 105..309
NoneNo IPR availableTMHMMTMhelixcoord: 386..408
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 62..203
e-value: 6.4E-16
score: 59.2
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 209..268
e-value: 7.1E-15
score: 54.5
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 257..278
score: 7.643
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 281..302
score: 5.078
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 233..254
score: 4.963
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 209..230
score: 9.09
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 185..206
score: 5.987
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 137..158
score: 4.84
IPR026906BspA type Leucine rich repeat regionPFAMPF13306LRR_5coord: 58..184
e-value: 7.3E-7
score: 29.2