SMED30003218

Overview
NameSMED30003218
Smed IDSMED30003218
Length (bp)1785
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003218 (SMED30003218) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003218 (SMED30003218) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003218 (SMED30003218) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003218 (SMED30003218) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003218

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30003218h1SMcG0003049 dd_Smed_v4_9752_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30003218h1SMcG0003049 dd_Smed_v4_9752_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30003218h1SMcG0003049 dd_Smed_v6_9752_0_1dd_Smed_v6PMID:29291974
Strand et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003218 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003235:14522..16371 +8770
v31.027262:1986..3836 -8721
Homology
[Close]
BLAST of SMED30003218 vs. Ensembl Human
Match: FBXL2 (F-box and leucine rich repeat protein 2 [Source:HGNC Symbol;Acc:HGNC:13598])

HSP 1 Score: 59.3066 bits (142), Expect = 9.492e-9
Identity = 38/128 (29.69%), Postives = 67/128 (52.34%), Query Frame = 1 
Query: 1321 NGLKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            + LKG+S   R L+ L+L  C  +T   +   ++    +  L ++G  ++ D+ LK I N   ++  +NL S S ITDEG++       +L+ L L  CS +T+  ++ L + CP +Q +    C H+
Sbjct:  146 SSLKGISEGCRNLEYLNLSWCDQITKDGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNYCHELVSLNLQSCSRITDEGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQILEAARCSHL 273
[Close]
BLAST of SMED30003218 vs. Ensembl Zebrafish
Match: fbxl2 (F-box and leucine-rich repeat protein 2 [Source:ZFIN;Acc:ZDB-GENE-030925-12])

HSP 1 Score: 50.0618 bits (118), Expect = 5.421e-6
Identity = 34/128 (26.56%), Postives = 63/128 (49.22%), Query Frame = 1 
Query: 1321 NGLKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            + LK LS   R L+ L+L  C  +T   +    +    +  LF++G  +++D  LK +     ++  IN+ S + ITD+G +       KL+ + +  CS IT+  ++ L + C  ++ +    C HV
Sbjct:  155 HALKALSEGCRMLENLNLSWCDQITSDGIEALSRGCTALRALFLRGCTQLDDTALKHLQKHCPELMTINMQSCTQITDDGFVSLCRGCHKLQMVCISGCSNITDASLTALGLNCQRLKILEAARCSHV 282
[Close]
BLAST of SMED30003218 vs. Ensembl Mouse
Match: Fbxl2 (F-box and leucine-rich repeat protein 2 [Source:MGI Symbol;Acc:MGI:1919429])

HSP 1 Score: 57.3806 bits (137), Expect = 3.108e-8
Identity = 37/128 (28.91%), Postives = 67/128 (52.34%), Query Frame = 1 
Query: 1321 NGLKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            + LKG+S   R L+ L+L  C  +T   +   ++    +  L ++G  ++ D+ LK I N   ++  +NL S S ITD+G++       +L+ L L  CS +T+  ++ L + CP +Q +    C H+
Sbjct:  146 SSLKGISEGCRNLEYLNLSWCDQITKEGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNHCHELVSLNLQSCSRITDDGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQVLEAARCSHL 273
[Close]
BLAST of SMED30003218 vs. Ensembl Mouse
Match: Fbxl2 (F-box and leucine-rich repeat protein 2 [Source:MGI Symbol;Acc:MGI:1919429])

HSP 1 Score: 57.3806 bits (137), Expect = 3.108e-8
Identity = 37/128 (28.91%), Postives = 67/128 (52.34%), Query Frame = 1 
Query: 1321 NGLKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            + LKG+S   R L+ L+L  C  +T   +   ++    +  L ++G  ++ D+ LK I N   ++  +NL S S ITD+G++       +L+ L L  CS +T+  ++ L + CP +Q +    C H+
Sbjct:  146 SSLKGISEGCRNLEYLNLSWCDQITKEGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNHCHELVSLNLQSCSRITDDGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQVLEAARCSHL 273
[Close]
BLAST of SMED30003218 vs. UniProt/SwissProt
Match: sp|Q5R3Z8|FBXL2_PONAB (F-box/LRR-repeat protein 2 OS=Pongo abelii OX=9601 GN=FBXL2 PE=2 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 4.363e-8
Identity = 38/128 (29.69%), Postives = 67/128 (52.34%), Query Frame = 1 
Query: 1321 NGLKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            + LKG+S   R L+ L+L  C  +T   +   ++    +  L ++G  ++ D+ LK I N   ++  +NL S S ITDEG++       +L+ L L  CS +T+  ++ L + CP +Q +    C H+
Sbjct:  146 SSLKGISEGCRNLEYLNLSWCDQITKDGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNYCHELVSLNLQSCSRITDEGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQILEAARCSHL 273
[Close]
BLAST of SMED30003218 vs. UniProt/SwissProt
Match: sp|Q9UKC9|FBXL2_HUMAN (F-box/LRR-repeat protein 2 OS=Homo sapiens OX=9606 GN=FBXL2 PE=1 SV=3)

HSP 1 Score: 59.3066 bits (142), Expect = 4.558e-8
Identity = 38/128 (29.69%), Postives = 67/128 (52.34%), Query Frame = 1 
Query: 1321 NGLKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            + LKG+S   R L+ L+L  C  +T   +   ++    +  L ++G  ++ D+ LK I N   ++  +NL S S ITDEG++       +L+ L L  CS +T+  ++ L + CP +Q +    C H+
Sbjct:  146 SSLKGISEGCRNLEYLNLSWCDQITKDGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNYCHELVSLNLQSCSRITDEGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQILEAARCSHL 273
[Close]
BLAST of SMED30003218 vs. UniProt/SwissProt
Match: sp|Q8BH16|FBXL2_MOUSE (F-box/LRR-repeat protein 2 OS=Mus musculus OX=10090 GN=Fbxl2 PE=1 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 2.174e-7
Identity = 37/128 (28.91%), Postives = 67/128 (52.34%), Query Frame = 1 
Query: 1321 NGLKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            + LKG+S   R L+ L+L  C  +T   +   ++    +  L ++G  ++ D+ LK I N   ++  +NL S S ITD+G++       +L+ L L  CS +T+  ++ L + CP +Q +    C H+
Sbjct:  146 SSLKGISEGCRNLEYLNLSWCDQITKEGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNHCHELVSLNLQSCSRITDDGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQVLEAARCSHL 273
[Close]
BLAST of SMED30003218 vs. TrEMBL
Match: A0A2C9JQ68 (F-box domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106063429 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 1.184e-8
Identity = 81/294 (27.55%), Postives = 135/294 (45.92%), Query Frame = 1 
Query:   76 MNSLPIEILTKISNYLSFSDRKSFALVCLRFNQAIYFKELWQNVTFVIGKAISMKIDTHFL-VILTNIKFFQ---LNIVPQELSEILKYTKNLLNLDLVICPNLFLNTENIDYYFRKHIDIDVKSVLMNLKGLSLSGNKTTLTNIGFNCFVENCCSLKKIDLSELDIGSLENIWNWSLDEIDLYSEKKWKFNKFLGNISEKSDRLSFSNIISKLISLVGYLEDINFCSTPIGHDELFILSTMPRLKLRVIRLDNCKNLDIKAINSLFHFQSHSLE--EISFINTFPVTINWFVS 939
            MN LP E+++ I  YL+F+DRK  +LV   +  A     L +NV           +    L   LT+I F +    +I    L +ILK    L+ LDL  C +LF +   ++    +HI   ++  L+NLK L L+G +  +T++ F   V  C +L++I L+  +I     I+   L  ++  S   ++F+ FL  + +                + G ++ I+   T + +D L  L+++  L L  I L NC  L  K +  L   Q HSL+  +IS       T  +F +
Sbjct:    1 MNYLPSELISHIMGYLNFNDRKEASLVNKAWYLAYLHPSLQRNVVVKCKPPEKGSLPQGLLRRRLTHIDFGEGRSESINEDSLIQILKECPGLMELDLSGCNHLFFSGRFLERESDRHI---LRKTLLNLKELKLTGLR-QMTDMTFLRLVSVCENLERISLASTNI-----IFGNQLSGLNQISPVIFQFSTFLKFVKD----------------IAGQIKSIDLSYTTVPNDALDALASIKGLVLDEIVLKNCTELSDKGVKFLVTKQ-HSLKILDISECRELGTTKAFFAT 268
[Close]
BLAST of SMED30003218 vs. TrEMBL
Match: E2LQF7 (F-box domain-containing protein OS=Moniliophthora perniciosa (strain FA553 / isolate CP02) OX=554373 GN=MPER_09163 PE=4 SV=1)

HSP 1 Score: 65.0846 bits (157), Expect = 1.262e-7
Identity = 33/114 (28.95%), Postives = 66/114 (57.89%), Query Frame = 1 
Query: 1351 RLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINLSG-SSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNC 1689
            RL+ L+L+GC +L+   LS  L S   +  + + GV   +D+ +  + +++R+++ INL G  +++DEG+     +   LR+++L     +T+K VS L   CP + ++ + +C
Sbjct:  151 RLERLTLVGCSDLSTSTLSRVLPSFPNLVAIDLTGVENTSDKAIIGLASVARRLQGINLGGCKNVSDEGVFALAKNCPLLRRVKLSGLETLTDKPVSTLAKSCPLLLEIDLNSC 264
[Close]
BLAST of SMED30003218 vs. TrEMBL
Match: A0A0W0EZC0 (F-box domain-containing protein OS=Moniliophthora roreri OX=221103 GN=WG66_18015 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 4.985e-7
Identity = 38/130 (29.23%), Postives = 72/130 (55.38%), Query Frame = 1 
Query: 1336 LSSFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINLSG-SSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNC----DHVERPI 1710
            LS   RL+ L+L+GC +L+   LS  L S   +  + + GV   +D+ +  + +++++++ INL G  +++ EG+     +   LR+++L     +T+K VS L   CPF+ ++ + +C    DH  R I
Sbjct:  146 LSKCDRLERLTLVGCSDLSTNTLSRVLPSFPNLVAIDLTGVENTSDKAIIGLASVAKRLQGINLGGCKNVSGEGVFALAKNCPLLRRVKLSGLETLTDKPVSALAKSCPFLLEIDLNSCKLITDHAIRDI 275
[Close]
BLAST of SMED30003218 vs. TrEMBL
Match: V2YRY5 (Scf e3 ubiquitin ligase complex f-box protein grra OS=Moniliophthora roreri (strain MCA 2997) OX=1381753 GN=Moror_8087 PE=4 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 5.204e-7
Identity = 38/130 (29.23%), Postives = 72/130 (55.38%), Query Frame = 1 
Query: 1336 LSSFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINLSG-SSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNC----DHVERPI 1710
            LS   RL+ L+L+GC +L+   LS  L S   +  + + GV   +D+ +  + +++++++ INL G  +++ EG+     +   LR+++L     +T+K VS L   CPF+ ++ + +C    DH  R I
Sbjct:  146 LSKCDRLERLTLVGCSDLSTNTLSRVLPSFPNLVAIDLTGVENTSDKAIIGLASVAKRLQGINLGGCKNVSGEGVFALAKNCPLLRRVKLSGLETLTDKPVSALAKSCPFLLEIDLNSCKLITDHAIRDI 275
[Close]
BLAST of SMED30003218 vs. TrEMBL
Match: A0A2R6RZN1 (F-box domain-containing protein OS=Phlebia centrifuga OX=98765 GN=PHLCEN_2v1643 PE=4 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 6.220e-7
Identity = 34/126 (26.98%), Postives = 71/126 (56.35%), Query Frame = 1 
Query: 1351 RLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINLSGS-SITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHVERPIVLNYY 1725
            RL+ L+LI C +LT   L+  L     +  L + GV +  D+ + ++ + +++++ INL G   +TD+G++   ++   LR+++L+   +IT++ VS L   CP + ++ + NC  +    V + +
Sbjct:  174 RLERLTLINCTSLTDSALTRVLPCCPNLVALDLTGVVETTDRVVVALASSTKRLQGINLCGCRKLTDKGVLALAANCPSLRRVKLNGVEQITDEAVSALAKSCPLLLEIDLNNCKKITDASVRDLW 299
[Close]
BLAST of SMED30003218 vs. Ensembl Cavefish
Match: fbxl2 (F-box and leucine-rich repeat protein 2 [Source:ZFIN;Acc:ZDB-GENE-030925-12])

HSP 1 Score: 51.6026 bits (122), Expect = 1.618e-6
Identity = 36/128 (28.12%), Postives = 64/128 (50.00%), Query Frame = 1 
Query: 1321 NGLKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            + LK LS   R L+ L+L  C  +T   +    +  N +  LF++G  +++D  LK +     ++  IN+ S + ITDEG +       KL+ + +  CS IT+  ++ L + C  ++ +    C HV
Sbjct:  143 HALKALSEGCRMLENLNLSWCDQITRDGIEALSRGCNGLKALFLRGCTQLDDTALKHLQKHCPELVTINMQSCTQITDEGFVSLCRGCHKLQMVCVSGCSNITDASLTALGLNCQRLKILEAARCSHV 270
[Close]
BLAST of SMED30003218 vs. Ensembl Sea Lamprey
Match: amn1 (antagonist of mitotic exit network 1 homolog (S. cerevisiae) [Source:NCBI gene;Acc:751744])

HSP 1 Score: 48.1358 bits (113), Expect = 2.071e-6
Identity = 29/114 (25.44%), Postives = 56/114 (49.12%), Query Frame = 1 
Query: 1354 LKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINLSGSSITDEGLIGFLSSAF--KLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNC 1689
            L+ + L  C  LT   + +   S   +  L ++G  ++ D  L ++   S  ++ IN + + +TDEG+I  ++ A    L ++ +D C  +T+K V  +   CP I+ +    C
Sbjct:  114 LRDVELSQCTELTDRAIIKLAASCPQLWILNLRGCHRLGDASLHALGRASSSLQFINFAKTQVTDEGVIALVNGACSKSLEEVHMDHCPSLTDKAVEAVLKSCPQIKYLHFHEC 227
[Close]
BLAST of SMED30003218 vs. Ensembl Nematostella
Match: EDO37271 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SG89])

HSP 1 Score: 51.6026 bits (122), Expect = 8.327e-7
Identity = 29/112 (25.89%), Postives = 59/112 (52.68%), Query Frame = 1 
Query: 1309 GNRKNGLKGLSSFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINLSGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVL 1644
            G     L+ L+S + L+ L+   C  ++   L    QS   + ++ V     + D+ ++++      + ++++S   +T+EGL  FL+S   L++LR+  CSK+T+ L+  L
Sbjct:  916 GVTDKALQSLASCKELEELNFSSCFQISDNGLVPLFQSCPRLLEVHVSSCYGVTDRSVQALAKSCPYLRDLDVSWCHVTNEGLEAFLTSPTSLKRLRIKCCSKVTDALIWKL 1027
[Close]
BLAST of SMED30003218 vs. Ensembl Medaka
Match: fbxl2 (F-box/LRR-repeat protein 2 [Source:NCBI gene;Acc:101157070])

HSP 1 Score: 54.6842 bits (130), Expect = 1.807e-7
Identity = 38/128 (29.69%), Postives = 64/128 (50.00%), Query Frame = 1 
Query: 1321 NGLKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            + LK LS   R L+TL+L  C  +T   +    +    +  LF++G  +++D  LK       ++  IN+ S + ITDEGL+       KL+ L +  C  IT+  ++ L + CP ++ +    C HV
Sbjct:  155 HSLKALSDGCRMLETLNLSWCDQITRDGIEALARGCMGLRALFLRGCTQLDDGALKHFQKHCPELTTINMQSCTQITDEGLVSLCRGCHKLQVLCVSGCGNITDASLTALGLNCPRLKILEAARCSHV 282
[Close]
BLAST of SMED30003218 vs. Ensembl Medaka
Match: fbxl20 (F-box/LRR-repeat protein 20 [Source:NCBI gene;Acc:101160411])

HSP 1 Score: 49.6766 bits (117), Expect = 7.296e-6
Identity = 35/126 (27.78%), Postives = 64/126 (50.79%), Query Frame = 1 
Query: 1327 LKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            LK LS     L+ L++  C  +T   +   ++S   +  LF++G  ++ D+ LK I     ++  +NL + S ITDEGLI       +L+ L +  C+ IT+ ++  L   CP ++ + +  C  +
Sbjct:  161 LKALSEGCHSLEQLNISWCDQVTKDGIQALVRSCPGLKGLFLKGCTQLEDEALKQIGAYCPELVTLNLQTCSQITDEGLITICRGCHRLQSLCVSGCANITDAILHALGQNCPRLRILEVARCSQL 286
[Close]
BLAST of SMED30003218 vs. Ensembl Medaka
Match: fbxl20 (F-box/LRR-repeat protein 20 [Source:NCBI gene;Acc:101160411])

HSP 1 Score: 49.6766 bits (117), Expect = 7.296e-6
Identity = 35/126 (27.78%), Postives = 64/126 (50.79%), Query Frame = 1 
Query: 1327 LKGLS-SFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINL-SGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHV 1698
            LK LS     L+ L++  C  +T   +   ++S   +  LF++G  ++ D+ LK I     ++  +NL + S ITDEGLI       +L+ L +  C+ IT+ ++  L   CP ++ + +  C  +
Sbjct:  161 LKALSEGCHSLEQLNISWCDQVTKDGIQALVRSCPGLKGLFLKGCTQLEDEALKQIGAYCPELVTLNLQTCSQITDEGLITICRGCHRLQSLCVSGCANITDAILHALGQNCPRLRILEVARCSQL 286
[Close]
BLAST of SMED30003218 vs. Planmine SMEST
Match: SMESG000027728.1 (SMESG000027728.1)

HSP 1 Score: 966.837 bits (2498), Expect = 0.000e+0
Identity = 503/514 (97.86%), Postives = 507/514 (98.64%), Query Frame = 1 
Query:  235 MKIDTHFLVILTNIKFFQLNIVPQELSEILKYTKNLLNLDLVICPNLFLNTENIDYYFRKHIDIDVKSVLMNLKGLSLSGNKTTLTNIGFNCFVENCCSLKKIDLSELDIGSLENIWNWSLDEIDLYSEKKWKFNKFLGNISEKSDRLSFSNIISKLISLVGYLEDINFCSTPIGHDELFILSTMPRLKLRVIRLDNCKNLDIKAINSLFHFQSHSLEEISFINTFPVTINWFVSNFINHNTLKRIRVRGTGQPELQLFENVRLHNIQISFIHLESCSIDDHFIDGLNQIKSIRKLYLLGCNFSKLEKNLLLMDELIELNFSRSYGFTFNDFVRSIFSLTXXXXXXXXXXXXXXXXXGGNRKNGLKGLSSFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINLSGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHVERPIVLNYYDSMFLYQWHSENHDIRI 1776
            MKIDTHFLVILTNIKFFQLNI+PQELSEILKYTKNLLNLDLVICPNLFLNTE IDYYFRKHIDIDVK VLMNLKGLSLSGNKTTLTNIGFNCFVENCCSLKKIDLSELDIGSL+NIWNWSLDEIDLYSEKKWKFNKFLGNISEKSDRLSFSNIISKLISLV YLEDINFCSTPIGHDELFILSTMPRLKLRVIRLDNCKNLDIKAINSL +FQSHSLEEISFINTFPVTINWFV NF NHNTLKRIRVRGTGQPELQLFENVRL NIQISFIHLESCSIDDHFIDGLNQIKSIRKLYL+GCNFSKLEKNLLLMDELIELNFSRSYGFTFNDFVRSIFSLTKIELLKLDWLKIDDDLIGGNRKNGLKGLSSFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINLSGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHVERPIVLNYYDSMFLYQWHSENHDIRI
Sbjct:    1 MKIDTHFLVILTNIKFFQLNIIPQELSEILKYTKNLLNLDLVICPNLFLNTEKIDYYFRKHIDIDVKRVLMNLKGLSLSGNKTTLTNIGFNCFVENCCSLKKIDLSELDIGSLDNIWNWSLDEIDLYSEKKWKFNKFLGNISEKSDRLSFSNIISKLISLVEYLEDINFCSTPIGHDELFILSTMPRLKLRVIRLDNCKNLDIKAINSLLYFQSHSLEEISFINTFPVTINWFVRNFFNHNTLKRIRVRGTGQPELQLFENVRLQNIQISFIHLESCSIDDHFIDGLNQIKSIRKLYLMGCNFSKLEKNLLLMDELIELNFSRSYGFTFNDFVRSIFSLTKIELLKLDWLKIDDDLIGGNRKNGLKGLSSFRRLKTLSLIGCGNLTGCVLSECLQSMNLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINLSGSSITDEGLIGFLSSAFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHVERPIVLNYYDSMFLYQWHSENHDIRI 514
The following BLAST results are available for this feature:
BLAST of SMED30003218 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
FBXL29.492e-929.69F-box and leucine rich repeat protein 2 [Source:HG... [more]
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BLAST of SMED30003218 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003218 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003218 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fbxl25.421e-626.56F-box and leucine-rich repeat protein 2 [Source:ZF... [more]
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BLAST of SMED30003218 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003218 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Fbxl23.108e-828.91F-box and leucine-rich repeat protein 2 [Source:MG... [more]
Fbxl23.108e-828.91F-box and leucine-rich repeat protein 2 [Source:MG... [more]
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BLAST of SMED30003218 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q5R3Z8|FBXL2_PONAB4.363e-829.69F-box/LRR-repeat protein 2 OS=Pongo abelii OX=9601... [more]
sp|Q9UKC9|FBXL2_HUMAN4.558e-829.69F-box/LRR-repeat protein 2 OS=Homo sapiens OX=9606... [more]
sp|Q8BH16|FBXL2_MOUSE2.174e-728.91F-box/LRR-repeat protein 2 OS=Mus musculus OX=1009... [more]
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BLAST of SMED30003218 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2C9JQ681.184e-827.55F-box domain-containing protein OS=Biomphalaria gl... [more]
E2LQF71.262e-728.95F-box domain-containing protein OS=Moniliophthora ... [more]
A0A0W0EZC04.985e-729.23F-box domain-containing protein OS=Moniliophthora ... [more]
V2YRY55.204e-729.23Scf e3 ubiquitin ligase complex f-box protein grra... [more]
A0A2R6RZN16.220e-726.98F-box domain-containing protein OS=Phlebia centrif... [more]
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BLAST of SMED30003218 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fbxl21.618e-628.13F-box and leucine-rich repeat protein 2 [Source:ZF... [more]
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BLAST of SMED30003218 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
amn12.071e-625.44antagonist of mitotic exit network 1 homolog (S. c... [more]
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BLAST of SMED30003218 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003218 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO372718.327e-725.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30003218 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
fbxl21.807e-729.69F-box/LRR-repeat protein 2 [Source:NCBI gene;Acc:1... [more]
fbxl207.296e-627.78F-box/LRR-repeat protein 20 [Source:NCBI gene;Acc:... [more]
fbxl207.296e-627.78F-box/LRR-repeat protein 20 [Source:NCBI gene;Acc:... [more]
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BLAST of SMED30003218 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000027728.10.000e+097.86SMESG000027728.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003218 ID=SMED30003218|Name=SMED30003218|organism=Schmidtea mediterranea sexual|type=transcript|length=1785bp
CCGATCTGTTAAAATATAAACTTATATTGAAAATGAACTGTTTGATATTT
AAAATTTCCAAATAATTATAATTCTATGAATTCCTTACCTATTGAAATTT
TGACAAAGATTTCTAATTATTTATCTTTTTCGGATAGGAAATCATTTGCG
TTAGTTTGTTTAAGATTTAACCAAGCTATATATTTTAAAGAATTGTGGCA
AAATGTCACTTTCGTTATCGGTAAAGCAATTTCAATGAAAATAGATACTC
ACTTTTTGGTTATTTTGACAAATATTAAATTTTTTCAATTGAATATTGTA
CCTCAAGAATTATCTGAAATATTAAAATATACAAAAAATCTATTAAATTT
AGATTTGGTAATCTGTCCGAATTTATTTTTAAACACTGAAAATATAGATT
ATTACTTCAGAAAACATATCGATATTGATGTTAAAAGTGTTTTAATGAAT
TTGAAAGGTTTGAGTCTTAGTGGAAATAAAACAACATTAACCAATATTGG
CTTCAATTGTTTTGTTGAAAATTGTTGTAGTCTTAAGAAAATAGATTTAT
CTGAACTTGATATTGGAAGCTTAGAAAATATATGGAATTGGTCATTAGAT
GAAATTGATTTATATTCGGAAAAAAAATGGAAATTTAATAAATTTCTTGG
TAATATTTCAGAAAAATCCGATCGATTATCATTTTCTAATATTATTAGCA
AATTGATTTCATTGGTTGGATATTTAGAAGACATAAACTTTTGCTCAACG
CCGATTGGCCACGATGAACTTTTTATATTGTCTACAATGCCTAGATTAAA
ATTACGTGTAATTCGATTGGATAATTGTAAAAATTTAGATATAAAAGCAA
TTAATTCATTGTTTCACTTTCAAAGTCATTCACTAGAAGAAATTAGTTTT
ATAAATACTTTTCCCGTAACCATAAATTGGTTTGTTAGTAATTTTATTAA
TCATAATACTCTTAAAAGGATTCGTGTCCGTGGAACTGGACAACCGGAAC
TGCAATTATTTGAAAATGTTCGATTGCATAATATTCAAATATCTTTTATT
CATTTAGAAAGCTGTTCCATCGATGATCACTTCATTGATGGCCTAAATCA
AATAAAATCCATTAGAAAACTTTATCTTTTGGGATGTAATTTCAGTAAAT
TAGAAAAAAATCTTTTGTTAATGGACGAATTGATTGAATTAAATTTCAGT
AGATCTTATGGATTTACATTCAATGATTTTGTTCGTTCGATATTTTCCCT
AACGAAAATAGAATTATTGAAATTGGATTGGTTGAAGATTGACGATGATT
TAATTGGTGGGAATAGAAAAAACGGACTAAAAGGATTGAGCTCATTTAGA
CGCTTGAAAACATTGAGTTTAATCGGATGCGGAAATCTGACTGGTTGCGT
GCTGAGTGAATGTTTACAGTCAATGAATTTAATGACAAAACTGTTTGTTC
AAGGCGTCAGGAAAATAAATGATCAATTTTTAAAAAGCATTCACAATTTG
TCTCGGCAAATTGAAGAAATAAATCTATCAGGGTCTTCGATTACTGATGA
AGGCTTGATTGGATTTCTTAGTTCGGCTTTTAAATTAAGGAAATTACGAT
TGGATGATTGCAGTAAAATAACGGAAAAGTTAGTATCTGTGTTGCCAGTT
GTTTGTCCATTTATACAGGATGTGAGTATGAGGAATTGTGATCATGTTGA
ACGTCCAATTGTATTGAATTATTACGATTCAATGTTTCTTTATCAATGGC
ACAGCGAAAATCACGATATTCGTATATAATCAATA
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protein sequence of SMED30003218-orf-1

>SMED30003218-orf-1 ID=SMED30003218-orf-1|Name=SMED30003218-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=568bp
MNSLPIEILTKISNYLSFSDRKSFALVCLRFNQAIYFKELWQNVTFVIGK
AISMKIDTHFLVILTNIKFFQLNIVPQELSEILKYTKNLLNLDLVICPNL
FLNTENIDYYFRKHIDIDVKSVLMNLKGLSLSGNKTTLTNIGFNCFVENC
CSLKKIDLSELDIGSLENIWNWSLDEIDLYSEKKWKFNKFLGNISEKSDR
LSFSNIISKLISLVGYLEDINFCSTPIGHDELFILSTMPRLKLRVIRLDN
CKNLDIKAINSLFHFQSHSLEEISFINTFPVTINWFVSNFINHNTLKRIR
VRGTGQPELQLFENVRLHNIQISFIHLESCSIDDHFIDGLNQIKSIRKLY
LLGCNFSKLEKNLLLMDELIELNFSRSYGFTFNDFVRSIFSLTKIELLKL
DWLKIDDDLIGGNRKNGLKGLSSFRRLKTLSLIGCGNLTGCVLSECLQSM
NLMTKLFVQGVRKINDQFLKSIHNLSRQIEEINLSGSSITDEGLIGFLSS
AFKLRKLRLDDCSKITEKLVSVLPVVCPFIQDVSMRNCDHVERPIVLNYY
DSMFLYQWHSENHDIRI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000025nervous system
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR036047F-box-like_dom_sf
IPR001810F-box_dom
IPR032675LRR_dom_sf
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005516calmodulin binding
Vocabulary: biological process
TermDefinition
GO:0010506regulation of autophagy
GO:0014066regulation of phosphatidylinositol 3-kinase signaling
GO:0016567protein ubiquitination
GO:0031146SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0019005SCF ubiquitin ligase complex
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 4..44
e-value: 7.2E-6
score: 35.5
IPR001810F-box domainPFAMPF12937F-box-likecoord: 2..46
e-value: 6.2E-7
score: 29.2
IPR001810F-box domainPROSITEPS50181FBOXcoord: 1..44
score: 12.359
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 1..186
e-value: 3.7E-13
score: 51.1
coord: 187..360
e-value: 1.4E-5
score: 26.2
coord: 361..551
e-value: 1.4E-20
score: 75.5
NoneNo IPR availablePANTHERPTHR13382MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR Bcoord: 207..544
NoneNo IPR availableSUPERFAMILYSSF52047RNI-likecoord: 82..358
NoneNo IPR availableSUPERFAMILYSSF52047RNI-likecoord: 321..553
IPR036047F-box-like domain superfamilySUPERFAMILYSSF81383F-box domaincoord: 2..50