SMED30003198

Overview
NameSMED30003198
Smed IDSMED30003198
Length (bp)2852
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003198 (SMED30003198) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003198 (SMED30003198) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003198 (SMED30003198) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003198 (SMED30003198) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003198

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30003198SMESG000050537.1 SmedASXL_016596SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30003198SMESG000050537.1 SmedASXL_016596SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30003198SMESG000050537.1 dd_Smed_v6_8384_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30003198 vs. TrEMBL
Match: U6A492 (NCOA5 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 9.694e-6
Identity = 43/164 (26.22%), Postives = 79/164 (48.17%), Query Frame = 3 
Query:  189 KPTFDMALIITHTSLISDICKLQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMNIHEVDDLVRD--DSYNTS-RQIKTLMEKPSKYPH--PKNEFHVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQR 665
            +P FD A+I+  + L+S    +++   ++   TTH+I + + S    C+ DL    + YA ++N          L +LY   +  + M ++     V    + Y    R +KT+  KP+  P   P      +++  Y L LL+D+RML ++EL  +  F+ +R
Sbjct:  175 RPGFDAAIIVMSSELLSYAETIESRLQRQIALTTHIIVLKEESHANACVEDLNNKGVLYAFLVNSLNEQHFSCTLRILYGTPQEHRNMPLNNALTFVARNFERYQKHVRDLKTIAPKPTFDPSRMPPGFIAPDDKIIYYLHLLADSRMLTIEELNEVIDFVQKR 338
[Close]
BLAST of SMED30003198 vs. Planmine SMEST
Match: SMESG000050537.1 (SMESG000050537.1)

HSP 1 Score: 1838.54 bits (4761), Expect = 0.000e+0
Identity = 904/917 (98.58%), Postives = 909/917 (99.13%), Query Frame = 3 
Query:   57 MEPREDEEISLKYKTVDDYMNGCPLSDPNVDLPFDHMQAELIFNKPTFDMALIITHTSLISDICKLQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMNIHEVDDLVRDDSYNTSRQIKTLMEKPSKYPHPKNEFHVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQRKAEMERSVAGQTVHIDERPLEACRQERLKYYIYEPIFNENQLVQRAEIPTRNPITTPEAQRSTTKFSIGPNVNATTSLYQPPSKYPIQSIPEPPIMNRAKIITNTFGSNLQQIKSITIARGQLKPFSYEVIQGGGILKTEAIKKNDSQREEYSKSNYFINQNLWREDDDIISLSSGSCTPPIEYDLIPISPPDEQITSSKVDLGKRKLATLDLSESNSLPKLHKIDLRKPEITASTLVIKNTPDVLVNMFRFIAHTMPYDLPHDNAILNIMINYCNDIQNGIMEPSLKIVKIDYESCKINGANEIVKSIYGIYEVAEKNVEDIVDLQYIWKKYFPDHLSNFSLLVVFYKFIKMLIIFRLFNSQSDRLQIISNTVEKLNLIANFDFYLPLLSDKQHLNCISELFGNTLESHSDLHTKYFDLPHISEIAISNKKLDNVIKKRKIEFVLSPLLKYFVNTLKSIHQQMKLAQTELVYKAIRTLRYCLRVIYPINSPTTVFIVPKTFSESGNNGYKDIQDEIKWLLDEIKYYQCVLFTEKHYDFINCVLDFSFVVEGIIXXXXXXXXXXXXXLVVLDTDQFIESFAAFEGMNLQYHADSEISCLKHFLKESWVQILQKAFRGLSTDGRGKRIVKEINNLLPELWGLKTFDEAVVDAAKCNYTEWTKIAQNNPTGWHESFSKVKFAGIVWLNTNQLSLNKAVEELQRCENMLRNEFLHPS 2807
            MEPREDEEISLKYKTVDDYMNGCPLSDPNVDLPFDH QAELIFNKPTFDMALIITHTSLISDIC+LQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMN+ EVDDLVRDDSYNTS QIK+LMEKPSKYPHPKNEFHVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQRKAEMERSVAGQTVHIDERPLEACRQERLKYYIYEPIFNENQLVQRA IP RNPITTPEAQRS+TKFSIGPNVN TTSLYQPPSKYPIQSIPEPPIMNRAKIITNTFGSNLQQIKSITIARGQLKPFSYEVIQGGGILKTEAIKKNDSQREEYSKSNYFINQNLWREDDDIISLSSGSCTPPIEYDLIPISPPDEQITSSKVDLGKRKLATLDLSESNSLPKLHKIDLRKPEITASTLVIKNTPDVLVNMFRFIAH MPYDLPHDNAILNIMINYCNDIQNGIMEPSLKIVKIDYESCKINGANEIVKSIYGIYEVAEKNVEDIVDLQYIWKKYFPDHLSNFSLLVVFYKFIKMLIIFRLFNSQSDRLQIISNTVEKLNLIANFDFYLPLLSDKQHLNCISELFGNTLESHSDLHTKYFDLPHISEIAIS KKLDNVIKKRKIEFVLSPLLKYFVNTLKSIHQQMKLAQTELVYKAIRTLRYCLRVIYPINSPTTVFIVPKTFSESGNNGYKDIQDEIKWLLDEIKYYQCVLFTEKHYDFINCVLDFSFVVEGIIDSDSSTSRSNTDSLVVLDTDQFIESFAAFEGMNLQYHADSEISCLKHFLKESWVQILQKAFRGLSTDG+GKRIVKEINNLLPELWGLKTFDEAVVDAAKCNYTEWTKIAQNNPTGWHESFSKVKFAGIVWLNTNQLSLNKAVEELQRCENMLRNEFLHPS
Sbjct:    1 MEPREDEEISLKYKTVDDYMNGCPLSDPNVDLPFDHKQAELIFNKPTFDMALIITHTSLISDICELQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMNLSEVDDLVRDDSYNTSIQIKSLMEKPSKYPHPKNEFHVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQRKAEMERSVAGQTVHIDERPLEACRQERLKYYIYEPIFNENQLVQRAAIPPRNPITTPEAQRSSTKFSIGPNVNTTTSLYQPPSKYPIQSIPEPPIMNRAKIITNTFGSNLQQIKSITIARGQLKPFSYEVIQGGGILKTEAIKKNDSQREEYSKSNYFINQNLWREDDDIISLSSGSCTPPIEYDLIPISPPDEQITSSKVDLGKRKLATLDLSESNSLPKLHKIDLRKPEITASTLVIKNTPDVLVNMFRFIAHAMPYDLPHDNAILNIMINYCNDIQNGIMEPSLKIVKIDYESCKINGANEIVKSIYGIYEVAEKNVEDIVDLQYIWKKYFPDHLSNFSLLVVFYKFIKMLIIFRLFNSQSDRLQIISNTVEKLNLIANFDFYLPLLSDKQHLNCISELFGNTLESHSDLHTKYFDLPHISEIAISTKKLDNVIKKRKIEFVLSPLLKYFVNTLKSIHQQMKLAQTELVYKAIRTLRYCLRVIYPINSPTTVFIVPKTFSESGNNGYKDIQDEIKWLLDEIKYYQCVLFTEKHYDFINCVLDFSFVVEGIIDSDSSTSRSNTDSLVVLDTDQFIESFAAFEGMNLQYHADSEISCLKHFLKESWVQILQKAFRGLSTDGKGKRIVKEINNLLPELWGLKTFDEAVVDAAKCNYTEWTKIAQNNPTGWHESFSKVKFAGIVWLNTNQLSLNKAVEELQRCENMLRNEFLHPS 917
[Close]
BLAST of SMED30003198 vs. Planmine SMEST
Match: SMESG000050537.1 (SMESG000050537.1)

HSP 1 Score: 1765.74 bits (4572), Expect = 0.000e+0
Identity = 876/917 (95.53%), Postives = 881/917 (96.07%), Query Frame = 3 
Query:   57 MEPREDEEISLKYKTVDDYMNGCPLSDPNVDLPFDHMQAELIFNKPTFDMALIITHTSLISDICKLQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMNIHEVDDLVRDDSYNTSRQIKTLMEKPSKYPHPKNEFHVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQRKAEMERSVAGQTVHIDERPLEACRQERLKYYIYEPIFNENQLVQRAEIPTRNPITTPEAQRSTTKFSIGPNVNATTSLYQPPSKYPIQSIPEPPIMNRAKIITNTFGSNLQQIKSITIARGQLKPFSYEVIQGGGILKTEAIKKNDSQREEYSKSNYFINQNLWREDDDIISLSSGSCTPPIEYDLIPISPPDEQITSSKVDLGKRKLATLDLSESNSLPKLHKIDLRKPEITASTLVIKNTPDVLVNMFRFIAHTMPYDLPHDNAILNIMINYCNDIQNGIMEPSLKIVKIDYESCKINGANEIVKSIYGIYEVAEKNVEDIVDLQYIWKKYFPDHLSNFSLLVVFYKFIKMLIIFRLFNSQSDRLQIISNTVEKLNLIANFDFYLPLLSDKQHLNCISELFGNTLESHSDLHTKYFDLPHISEIAISNKKLDNVIKKRKIEFVLSPLLKYFVNTLKSIHQQMKLAQTELVYKAIRTLRYCLRVIYPINSPTTVFIVPKTFSESGNNGYKDIQDEIKWLLDEIKYYQCVLFTEKHYDFINCVLDFSFVVEGIIXXXXXXXXXXXXXLVVLDTDQFIESFAAFEGMNLQYHADSEISCLKHFLKESWVQILQKAFRGLSTDGRGKRIVKEINNLLPELWGLKTFDEAVVDAAKCNYTEWTKIAQNNPTGWHESFSKVKFAGIVWLNTNQLSLNKAVEELQRCENMLRNEFLHPS 2807
            MEPREDEEISLKYKTVDDYMNGCPLSDPNVDLPFDH QAELIFNKPTFDMALIITHTSLISDIC+LQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMN+ EVDDLVRDDSYNTS QIK+LMEKPSKYPHPKNEFHVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQRKAEMERSVAGQTVHIDERPLEACRQERLKYYIYEPIFNENQLVQRA IP RNPITTPEAQRS+TKFSIGPNVN TTSLYQPPSKYPIQSIPEPPIMNRAKIITNTFGSNLQQIKSITIARGQLKPFSYEVIQGGGILKTEAIKKNDSQREEYSKSNYFINQNLWREDDDIISLSSG                            KRKLATLDLSESNSLPKLHKIDLRKPEITASTLVIKNTPDVLVNMFRFIAH MPYDLPHDNAILNIMINYCNDIQNGIMEPSLKIVKIDYESCKINGANEIVKSIYGIYEVAEKNVEDIVDLQYIWKKYFPDHLSNFSLLVVFYKFIKMLIIFRLFNSQSDRLQIISNTVEKLNLIANFDFYLPLLSDKQHLNCISELFGNTLESHSDLHTKYFDLPHISEIAIS KKLDNVIKKRKIEFVLSPLLKYFVNTLKSIHQQMKLAQTELVYKAIRTLRYCLRVIYPINSPTTVFIVPKTFSESGNNGYKDIQDEIKWLLDEIKYYQCVLFTEKHYDFINCVLDFSFVVEGIIDSDSSTSRSNTDSLVVLDTDQFIESFAAFEGMNLQYHADSEISCLKHFLKESWVQILQKAFRGLSTDG+GKRIVKEINNLLPELWGLKTFDEAVVDAAKCNYTEWTKIAQNNPTGWHESFSKVKFAGIVWLNTNQLSLNKAVEELQRCENMLRNEFLHPS
Sbjct:    1 MEPREDEEISLKYKTVDDYMNGCPLSDPNVDLPFDHKQAELIFNKPTFDMALIITHTSLISDICELQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMNLSEVDDLVRDDSYNTSIQIKSLMEKPSKYPHPKNEFHVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQRKAEMERSVAGQTVHIDERPLEACRQERLKYYIYEPIFNENQLVQRAAIPPRNPITTPEAQRSSTKFSIGPNVNTTTSLYQPPSKYPIQSIPEPPIMNRAKIITNTFGSNLQQIKSITIARGQLKPFSYEVIQGGGILKTEAIKKNDSQREEYSKSNYFINQNLWREDDDIISLSSGC---------------------------KRKLATLDLSESNSLPKLHKIDLRKPEITASTLVIKNTPDVLVNMFRFIAHAMPYDLPHDNAILNIMINYCNDIQNGIMEPSLKIVKIDYESCKINGANEIVKSIYGIYEVAEKNVEDIVDLQYIWKKYFPDHLSNFSLLVVFYKFIKMLIIFRLFNSQSDRLQIISNTVEKLNLIANFDFYLPLLSDKQHLNCISELFGNTLESHSDLHTKYFDLPHISEIAISTKKLDNVIKKRKIEFVLSPLLKYFVNTLKSIHQQMKLAQTELVYKAIRTLRYCLRVIYPINSPTTVFIVPKTFSESGNNGYKDIQDEIKWLLDEIKYYQCVLFTEKHYDFINCVLDFSFVVEGIIDSDSSTSRSNTDSLVVLDTDQFIESFAAFEGMNLQYHADSEISCLKHFLKESWVQILQKAFRGLSTDGKGKRIVKEINNLLPELWGLKTFDEAVVDAAKCNYTEWTKIAQNNPTGWHESFSKVKFAGIVWLNTNQLSLNKAVEELQRCENMLRNEFLHPS 890
[Close]
BLAST of SMED30003198 vs. Planmine SMEST
Match: SMESG000050533.1 (SMESG000050533.1)

HSP 1 Score: 372.859 bits (956), Expect = 8.349e-119
Identity = 195/478 (40.79%), Postives = 308/478 (64.44%), Query Frame = 3 
Query: 1383 KNTPDVLVNMFRFIAHTMPYDLPHDNAILNIMINYCNDIQNGIMEPSLKIVKIDYESCKINGANEIVKSIYGIYEVAEKNVEDIVDLQYIWKKYFPDHLSNFSLLVVFYKFIKMLIIFRLFNSQSDRLQIISNTVEKLNLIANFDFYLPLLS-DKQHLNCISELFGNTLESHSDLHTKYFDLPHISEIAISNKKLDNVIKKRKIEFVLSPLLKYFVNTLKSIHQQMKLAQTELVYKAIRTLRYCLRVIYPINSPTTVFIVPKTF---SESGNNGYKDIQDEIKWLLDEIKYYQCVLFTEKHYDFINCVLDFSFVVEGIIXXXX--XXXXXXXXXLVVLDTDQFIESFAAFEG-MNLQYHADSEISCLKHFLKESWVQILQKAFRGLSTDGRGKRIVKEINNLLPELWGLKTFDEAVVDAAKCNYTEWTKIAQNNPTGWHESFSKVKFAGIVWLNTNQLSLNKAVEELQRCENMLRNEF 2795
            K++PD L+N+F  + + +P  LP DN +  I+ N C  IQ GI EP+L+ +KI++ESCK NG +EI+ +IY I+   EK V +I  +++IW KYFP  LSNF+L+++FY+F+K+L+++ +FN      +++ + +EK+++I NF+F   + +  K+ L+C S  F    + H  LH  Y  L +   + +++KKLD  IKK+KIEF + PLL YF++++ SIH +M+  Q E+VY AIRTLR+CLR I  +    T F  P  F   +++ ++   +++ EI WL  E+KYYQCVLFT+KHYDFINC+LDFS+ +E  I  +S     +   + LV  +  QF+++F AF+  + +QYH +SE+  L   LK  W+ IL +AF G   +  G RI+KEIN LLP  WGL TF+EA+++++K  Y  + K+A+ +P+GW+  FSK K   + WL   QL ++  +E L   + +L +++
Sbjct:   38 KSSPDGLINLFALVLNLVPLKLPIDNGVCTILTNSCVTIQEGIQEPALRNIKINHESCKANGVSEIIANIYSIFNEPEKEVLNISFMKFIWVKYFPQELSNFNLMILFYRFVKLLMLYTMFNKSLVTKKLVIDVIEKIDVIVNFNFKSSVSTFKKRQLSCFSHKFDKYFKEHDQLHVLYSKLSNSKSLVVNSKKLDITIKKKKIEFTVGPLLNYFISSINSIHNKMEPFQIEVVYGAIRTLRFCLRKI-NLKLVQTSFTKPYLFDFKTKNMDHHCTEVKKEINWLCKELKYYQCVLFTDKHYDFINCILDFSWRLECTIKVNSDPLQPKPICEPLVFHNPMQFVQTFTAFQCPIFIQYHPESELEYLNTCLKTKWIGILHEAFIGSLPEPCGARIIKEINVLLPPTWGLFTFNEAIIESSKMYYERFQKLAETDPSGWYTKFSKTKHCAMNWLIQYQLPVD-GLEVLHDYQYVLESKY 513
[Close]
BLAST of SMED30003198 vs. Planmine SMEST
Match: SMESG000050535.1 (SMESG000050535.1)

HSP 1 Score: 119.398 bits (298), Expect = 7.693e-29
Identity = 71/176 (40.34%), Postives = 109/176 (61.93%), Query Frame = 3 
Query:  174 ELIFNKPTFDMALIITHTSLISDICKLQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMNIHEVDDLVRDDSYNTSRQIKTLMEKPSKYPH---PKN-EF-HVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQRKAEMERS 686
            ELIFN    D+A I+TH  L+S + KLQN+F  + KF+T+  YI D   LKF +RDLK H ++YA+++++    +   DLYVL+   E+   + + +V  LVR D  N   ++K L   PSK+PH   P++ E+  +N++ RY  CLLSD+R L ++EL  +N +L  RK  ++ S
Sbjct:   27 ELIFNNSMCDIAFIVTHQPLMSKLDKLQNYFQSELKFSTYCTYIEDNESLKFYLRDLKTHDVSYALLLHDSFENRNFYDLYVLHK-DEVLPNITLEKVISLVRSDFDNMMSKVKILKNIPSKWPHSVKPEDLEYSKINDKLRYYFCLLSDSRTLSIEELNYVNDYLVTRKELLQIS 201
[Close]
BLAST of SMED30003198 vs. Planmine SMEST
Match: SMESG000010804.1 (SMESG000010804.1)

HSP 1 Score: 61.2326 bits (147), Expect = 3.082e-9
Identity = 43/164 (26.22%), Postives = 79/164 (48.17%), Query Frame = 3 
Query:  189 KPTFDMALIITHTSLISDICKLQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHRITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMNIHEVDDLVRD--DSYNTS-RQIKTLMEKPSKYPH--PKNEFHVNNETRYQLCLLSDNRMLKMQELQNLNRFLCQR 665
            +P FD A+I+  + L+S    +++   ++   TTH+I + + S    C+ DL    + YA ++N          L +LY   +  + M ++     V    + Y    R +KT+  KP+  P   P      +++  Y L LL+D+RML ++EL  +  F+ +R
Sbjct:  224 RPGFDAAIIVMSSELLSYAETIESRLQRQIALTTHIIVLKEESHANACVEDLNNKGVLYAFLVNSLNEQHFSCTLRILYGTPQEHRNMPLNNALTFVARNFERYQKHVRDLKTIAPKPTFDPSRMPPGFIAPDDKIIYYLHLLADSRMLTIEELNEVIDFVQKR 387
The following BLAST results are available for this feature:
BLAST of SMED30003198 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
U6A4929.694e-626.22NCOA5 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1[more]
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BLAST of SMED30003198 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003198 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000050537.10.000e+098.58SMESG000050537.1[more]
SMESG000050537.10.000e+095.53SMESG000050537.1[more]
SMESG000050533.18.349e-11940.79SMESG000050533.1[more]
SMESG000050535.17.693e-2940.34SMESG000050535.1[more]
SMESG000010804.13.082e-926.22SMESG000010804.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003198 ID=SMED30003198|Name=SMED30003198|organism=Schmidtea mediterranea sexual|type=transcript|length=2852bp
TATCAATAATTTTAATTTCACGTAATTATTATTATAATATCTAGTTATTG
AATATTATGGAACCTAGAGAAGATGAAGAAATATCATTAAAATATAAAAC
AGTTGATGATTATATGAATGGTTGTCCCTTAAGTGATCCCAATGTCGACT
TACCTTTTGACCATATGCAAGCAGAACTAATATTTAATAAGCCAACATTT
GACATGGCTCTAATTATCACCCATACTAGTTTAATATCGGATATTTGTAA
ACTGCAAAATTTTTTCCATAAAAAACATAAATTTACAACTCACGTTATAT
ATATACCAGATGTGAGTAAGTTAAAGTTTTGTATTCGGGATTTAAAATTG
CATCGAATAACATATGCCATTGTTATTAATGAATATGGTTTAATTCAAGG
CAAAATGGATTTATATGTTCTTTATCCGATTATTGAAATTCAAAAAAGAA
TGAATATTCATGAGGTGGATGATCTTGTTAGAGATGATTCTTACAATACA
TCACGACAAATAAAAACCCTAATGGAAAAACCGTCTAAATATCCTCATCC
AAAAAATGAGTTTCACGTGAACAATGAAACCCGTTACCAGTTGTGTTTAC
TATCAGACAATCGAATGCTAAAAATGCAAGAACTACAGAATTTGAATCGC
TTTTTATGTCAACGTAAAGCGGAAATGGAGAGATCTGTAGCTGGGCAAAC
GGTGCATATAGACGAACGACCACTGGAAGCTTGTAGACAGGAAAGATTGA
AGTATTATATCTACGAACCAATTTTCAATGAAAATCAATTAGTACAGAGG
GCAGAAATTCCAACAAGAAACCCAATAACTACTCCAGAGGCACAACGATC
TACAACGAAGTTTAGTATTGGGCCGAATGTAAATGCTACTACGTCATTAT
ACCAACCCCCATCTAAATATCCTATTCAATCTATTCCCGAACCACCAATC
ATGAATAGAGCTAAAATAATAACCAATACCTTCGGTTCTAATTTACAACA
AATCAAGAGCATTACTATTGCAAGAGGGCAACTAAAGCCGTTTTCATATG
AAGTCATTCAAGGAGGAGGAATATTGAAAACTGAGGCAATCAAGAAAAAT
GATTCTCAGAGAGAAGAATATTCTAAATCAAATTACTTTATAAACCAGAA
TTTATGGAGAGAAGATGATGATATCATCTCCTTGTCAAGTGGATCATGCA
CACCACCGATAGAATATGATCTTATTCCCATTTCGCCTCCTGATGAACAA
ATAACGTCTTCTAAAGTTGATTTAGGCAAAAGAAAATTGGCAACTTTGGA
TTTATCAGAGAGTAATTCGTTGCCAAAATTACATAAAATAGATCTAAGGA
AACCTGAAATCACAGCATCGACACTCGTTATTAAAAATACTCCTGATGTT
TTAGTGAACATGTTCAGATTCATTGCACATACCATGCCGTACGATTTGCC
GCATGACAACGCAATTCTCAATATAATGATAAATTATTGCAATGATATTC
AAAACGGAATAATGGAACCAAGTCTAAAAATCGTGAAAATCGATTACGAG
AGTTGTAAAATAAATGGAGCCAATGAAATAGTAAAAAGTATTTATGGCAT
TTATGAAGTGGCTGAGAAAAACGTTGAAGACATTGTTGATTTACAATACA
TTTGGAAAAAGTATTTTCCAGATCATCTTTCGAACTTCAGTCTACTTGTG
GTATTTTATAAATTCATAAAAATGCTTATTATCTTCAGGTTGTTCAATTC
TCAATCAGATAGATTACAAATAATATCTAATACGGTTGAAAAACTCAATT
TGATAGCTAATTTTGATTTCTATTTGCCACTCTTGTCAGACAAGCAGCAC
TTAAATTGCATTTCCGAGTTATTTGGCAATACATTAGAAAGTCACAGTGA
TCTCCATACTAAATATTTTGATTTACCGCATATAAGTGAAATTGCGATTT
CCAATAAAAAGTTAGACAATGTTATCAAAAAAAGAAAAATTGAATTCGTC
CTTAGTCCACTGCTCAAATATTTTGTAAACACCCTGAAATCCATTCACCA
ACAAATGAAACTCGCTCAAACCGAATTGGTTTACAAAGCTATCAGAACTT
TGCGATATTGTTTGAGAGTAATTTACCCGATCAATTCTCCAACTACAGTT
TTTATTGTCCCTAAAACCTTTTCTGAATCTGGAAATAATGGATATAAAGA
TATTCAAGATGAAATTAAGTGGCTACTTGATGAAATAAAATACTATCAAT
GCGTGTTGTTTACGGAAAAACATTATGATTTTATCAACTGTGTACTGGAT
TTCTCTTTTGTGGTCGAAGGAATTATAGATTCTGATTCCTCTACGTCTAG
ATCAAATACGGATTCTTTAGTTGTCCTTGATACTGATCAATTTATCGAAT
CCTTTGCTGCGTTTGAAGGCATGAATTTACAATATCATGCAGATTCTGAG
ATTTCATGTTTAAAACATTTCCTTAAAGAATCTTGGGTTCAAATTCTTCA
AAAGGCTTTTAGAGGATTATCAACTGATGGAAGGGGTAAAAGAATCGTGA
AGGAAATCAATAATTTACTGCCGGAATTGTGGGGGTTGAAAACATTCGAT
GAAGCTGTTGTAGATGCCGCTAAATGCAACTATACAGAATGGACGAAAAT
CGCCCAGAATAATCCAACAGGCTGGCATGAATCATTCAGCAAAGTTAAAT
TTGCTGGGATTGTTTGGCTGAATACTAATCAACTTTCATTGAATAAAGCT
GTTGAAGAGCTTCAAAGATGTGAAAATATGCTGAGAAATGAATTTTTACA
TCCATCATAAAATAATTTTCAAAATTTGTATGTTATAAATATAAATATGT
TT
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protein sequence of SMED30003198-orf-1

>SMED30003198-orf-1 ID=SMED30003198-orf-1|Name=SMED30003198-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=918bp
MEPREDEEISLKYKTVDDYMNGCPLSDPNVDLPFDHMQAELIFNKPTFDM
ALIITHTSLISDICKLQNFFHKKHKFTTHVIYIPDVSKLKFCIRDLKLHR
ITYAIVINEYGLIQGKMDLYVLYPIIEIQKRMNIHEVDDLVRDDSYNTSR
QIKTLMEKPSKYPHPKNEFHVNNETRYQLCLLSDNRMLKMQELQNLNRFL
CQRKAEMERSVAGQTVHIDERPLEACRQERLKYYIYEPIFNENQLVQRAE
IPTRNPITTPEAQRSTTKFSIGPNVNATTSLYQPPSKYPIQSIPEPPIMN
RAKIITNTFGSNLQQIKSITIARGQLKPFSYEVIQGGGILKTEAIKKNDS
QREEYSKSNYFINQNLWREDDDIISLSSGSCTPPIEYDLIPISPPDEQIT
SSKVDLGKRKLATLDLSESNSLPKLHKIDLRKPEITASTLVIKNTPDVLV
NMFRFIAHTMPYDLPHDNAILNIMINYCNDIQNGIMEPSLKIVKIDYESC
KINGANEIVKSIYGIYEVAEKNVEDIVDLQYIWKKYFPDHLSNFSLLVVF
YKFIKMLIIFRLFNSQSDRLQIISNTVEKLNLIANFDFYLPLLSDKQHLN
CISELFGNTLESHSDLHTKYFDLPHISEIAISNKKLDNVIKKRKIEFVLS
PLLKYFVNTLKSIHQQMKLAQTELVYKAIRTLRYCLRVIYPINSPTTVFI
VPKTFSESGNNGYKDIQDEIKWLLDEIKYYQCVLFTEKHYDFINCVLDFS
FVVEGIIDSDSSTSRSNTDSLVVLDTDQFIESFAAFEGMNLQYHADSEIS
CLKHFLKESWVQILQKAFRGLSTDGRGKRIVKEINNLLPELWGLKTFDEA
VVDAAKCNYTEWTKIAQNNPTGWHESFSKVKFAGIVWLNTNQLSLNKAVE
ELQRCENMLRNEFLHPS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: molecular function
TermDefinition
GO:0000166nucleotide binding
GO:0003676nucleic acid binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 190..210