SMED30003184

Overview
NameSMED30003184
Smed IDSMED30003184
Length (bp)1099
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003184 (SMED30003184) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003184 (SMED30003184) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003184 (SMED30003184) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003184 (SMED30003184) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003184

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
oocyteSMED30003184h1SMcG0008680 Contig30958newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30003184h1SMcG0008680 Contig30958uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30003184h1SMcG0008680 Contig30958newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30003184h1SMcG0008680 Contig30958uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003184 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001068:30965..32088 +5331
v31.004684:14311..15434 +5323
v31.004235:11675..12673 +4219
v31.007469:5282..7874 -2535
Homology
[Close]
BLAST of SMED30003184 vs. Planmine SMEST
Match: SMESG000047667.1 (SMESG000047667.1)

HSP 1 Score: 40.4318 bits (93), Expect = 9.256e-9
Identity = 16/22 (72.73%), Postives = 21/22 (95.45%), Query Frame = 2 
Query:  980 DVDRTTLTVPNRFAGRYRRGLY 1045
            DV+RTTLTVP++FAGRYR+ L+
Sbjct: 1568 DVERTTLTVPSKFAGRYRQALW 1589

HSP 2 Score: 37.7354 bits (86), Expect = 9.256e-9
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = 1 
Query: 1036 RFISISTYGTPEWPNRDPESR 1098
            R  SISTYGTPEWPNR+P+ R
Sbjct: 1591 RGYSISTYGTPEWPNRNPDRR 1611
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BLAST of SMED30003184 vs. Planmine SMEST
Match: SMESG000051105.1 (SMESG000051105.1)

HSP 1 Score: 55.0694 bits (131), Expect = 1.093e-8
Identity = 24/36 (66.67%), Postives = 29/36 (80.56%), Query Frame = 3 
Query:  321 RSNSNNNLMHSHERVRTEGCSAGKADLGLIFECVLI 428
            R  S++N MH+HE++RTE CS GKADLG I ECVLI
Sbjct:  169 RDASSSNPMHAHEKIRTEECSTGKADLGSISECVLI 204
[Close]
BLAST of SMED30003184 vs. Planmine SMEST
Match: SMESG000051105.1 (SMESG000051105.1)

HSP 1 Score: 54.6842 bits (130), Expect = 3.599e-8
Identity = 24/36 (66.67%), Postives = 29/36 (80.56%), Query Frame = 3 
Query:  321 RSNSNNNLMHSHERVRTEGCSAGKADLGLIFECVLI 428
            R  S++N MH+HE++RTE CS GKADLG I ECVLI
Sbjct:  251 RDASSSNPMHAHEKIRTEECSTGKADLGSISECVLI 286
[Close]
BLAST of SMED30003184 vs. Planmine SMEST
Match: SMESG000014495.1 (SMESG000014495.1)

HSP 1 Score: 42.743 bits (99), Expect = 4.016e-7
Identity = 18/22 (81.82%), Postives = 21/22 (95.45%), Query Frame = 2 
Query:  980 DVDRTTLTVPNRFAGRYRRGLY 1045
            DV+RTTLTVPNRFAGRYR+ L+
Sbjct:   68 DVNRTTLTVPNRFAGRYRQALW 89

HSP 2 Score: 30.0314 bits (66), Expect = 4.016e-7
Identity = 13/17 (76.47%), Postives = 14/17 (82.35%), Query Frame = 1 
Query: 1036 RFISISTYGTPEWPNRD 1086
            R  SISTYGTPEWP R+
Sbjct:   91 RGYSISTYGTPEWPCRN 107
The following BLAST results are available for this feature:
BLAST of SMED30003184 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003184 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000047667.19.256e-972.73SMESG000047667.1[more]
SMESG000051105.11.093e-866.67SMESG000051105.1[more]
SMESG000051105.13.599e-866.67SMESG000051105.1[more]
SMESG000014495.14.016e-781.82SMESG000014495.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003184 ID=SMED30003184|Name=SMED30003184|organism=Schmidtea mediterranea sexual|type=transcript|length=1099bp
GTCTCGAGAAAATCCTAATCCAAAACTGACATACAAACTTGGACAGGAGA
AATCGTAGAATATTTGTTATGGAGACAAAAATCTGCTGTGAAATCTATCA
CCCTCACGATCAATGGAAGAGAAACTCTGTGAGCAGCCAATCACAAACCG
CCACTGCCAATTATATCTCTCACCAGCCCATGTGACAGAAAAACGAACCA
ATATACTATACGCGAACTGAATTACAATGGAGGTCATACTTATCTCGTTC
TCAATCGAATTTATGTCTAAATCAACGGCACACAATTCAGAGCAATACGT
AAACGGGCACAGATTCATCGAGATCCAACTCCAATAACAATCTAATGCAT
TCCCATGAAAGAGTTCGCACGGAAGGATGCTCGGCAGGTAAGGCCGATCT
TGGCTTAATCTTTGAATGCGTGTTAATTTAATATAATTTTCTTTGGAGTT
ATAATTATTCAATTTAAAAAGTTAGACCTTTTACGTAATCGATTTTTGTC
CGTAACCCAGTTTCGTAAAAGTAAACACATAATAGTGATTCTGTACACCA
TTTATTTTCATCGTGGACAGATTTTTTGATTAGAAATGGTCGCCTAATGA
TAGCCATGAAATGATGAAATTAGTTTCTGATGTCGAACTTCTCCACCGAA
TGCAATGCTCAGCTCGCGATAACAAGTAAATCACCGAGGTAAATCACTCG
AGTATCACTCGACTAGCTTAGCCGATCTACGATTGTAAAACATGAATGTA
TAATCCACCCATTAGAAAAGTAGACGGAAAGAGAACCAGCAAGTCGTTAA
TTGAATAGATAGGTGCTCATTCGTAGCAATTCTATATTTTGGATTATACC
CATATTTCGGAAATGGCTAATCAAGTATATGTTCCGTGATCAGTGTGGAA
CTAGACGAGTAGCAATTGAGCCGTGACTTAAATGAATTTATTATTAACAT
TAACAAATACATCCAGCAGTAAATGGGTCGACGTCGATCGCACAACATTA
ACGGTTCCTAATAGGTTCGCTGGGAGATATCGCAGAGGTTTATATCCATT
TCGACTTATGGTACTCCCGAATGGCCGAATCGAGATCCAGAAAGTCGAA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000231vitelline gland
Vocabulary: INTERPRO
TermDefinition
IPR011107PPI_Ypi1