Reverse transcriptase domain-containing protein
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30003181 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A5S6QWV2 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1) HSP 1 Score: 60.8474 bits (146), Expect = 5.921e-10 Identity = 39/92 (42.39%), Postives = 54/92 (58.70%), Query Frame = 3
Query: 234 VIF*CGKVKTLTISY*EKYFRRIKIEG*N--PQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKN 503
+IF G+ T + E+ R++ G P KC+L E+ FLGH V+GKGI T+ K VE E P+C+ LR F+GL +YYR F+KN
Sbjct: 351 IIF--GRTTTEQMERLEEVLNRLQAAGLKVKPSKCKLMTQEVVFLGHVVSGKGISTDPLKCATVENWEPPRCTNELRQFIGLASYYRKFVKN 440 HSP 2 Score: 32.7278 bits (73), Expect = 5.921e-10 Identity = 35/139 (25.18%), Postives = 60/139 (43.17%), Query Frame = 1
Query: 547 CE-SFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVI-A*RPT**PRXXXXXXXXXXXXXESCLW-----------*KFIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYRNRK 924
CE +FQ + RL IL ++ F+ DA+ + LSQV +D E + A R P L + +W +F +RT H L+++N + P + + WL+ L+E++ + +R K
Sbjct: 464 CERAFQEMKRRLLTAPILKFPDFNKTFVLDVDASNDGLGAVLSQVHDDGEHPIAYASRALTKPERRYCATRRELL---ALVWASKHFRPYLYGTRFKVRTDHNCLIWLNNFREPEGQIAR-WLERLAEFDMEICHRPGK 598
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A0V1A064 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Trichinella patagoniensis OX=990121 GN=pol PE=4 SV=1) HSP 1 Score: 48.9062 bits (115), Expect = 9.344e-10 Identity = 29/76 (38.16%), Postives = 44/76 (57.89%), Query Frame = 3
Query: 282 EKYFRRIKIEG*N--PQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKN 503
E+ +RI+ G P KC L E+ FLGH V+ G++T+ K E+V P + +R+FLG +YYR F+K+
Sbjct: 535 EEVLQRIRQSGLKLKPAKCRLCAKEIAFLGHIVSRDGVRTDPSKTEKVASWPTPTSTSEVRTFLGFASYYRRFVKS 610 HSP 2 Score: 43.8986 bits (102), Expect = 9.344e-10 Identity = 38/122 (31.15%), Postives = 53/122 (43.44%), Query Frame = 1
Query: 550 ESFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVIA*RPT**PRXXXXXXXXXXXXXESCLW*KFIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYR 915
E+FQ L L G IL R FI TDA+ I LSQ + + E VIA R + L F +RT H SL ++ K P + + WL++L EY+ + +R
Sbjct: 617 EAFQRLKRALTTGQILAFPRFDIPFIIDTDASETGIGAVLSQKHDPEGERVIAYA----SRTLSKTERKYSTTRKELLSIGFTLRTDHDSLTWLRNFKEPEGQVSR-WLEHLQEYDMEVVHR 733
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A085MDW8 (Uncharacterized protein OS=Trichuris suis OX=68888 GN=M513_03754 PE=4 SV=1) HSP 1 Score: 58.5362 bits (140), Expect = 2.243e-9 Identity = 30/61 (49.18%), Postives = 41/61 (67.21%), Query Frame = 3
Query: 321 PQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKN 503
P KC++ TE+ FLGH ++ KGI T+ K VE+ P+C+ LR FLGL +YYR F+KN
Sbjct: 714 PSKCKVMTTEVLFLGHVISQKGISTDPSKCAAVERWNPPRCTNELRQFLGLTSYYRKFVKN 774 HSP 2 Score: 33.113 bits (74), Expect = 2.243e-9 Identity = 36/131 (27.48%), Postives = 61/131 (46.56%), Query Frame = 1
Query: 550 ESFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYV-IA*RPT**P-RXXXXXXXXXXXXXESC-------LW*KFIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYR 915
++FQ L RL IL L ++ F DA+ I LSQ ED E V A R P R +L ++ +C +F +RT H +L+++ + + P + + WL+ L+E++ + +R
Sbjct: 800 QAFQQLKQRLLSAPILRLPDFTKPFTLDVDASGEGIGAVLSQRYEDGEHPVAYASRALTKPERRYCATRRELLALVWACKHFRPYLYGARFSVRTDHNALIWLKSFREPEGQVAR-WLERLAEFDMEVTHR 929
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A0V1GTF0 (Transposon Ty3-I Gag-Pol polyprotein OS=Trichinella zimbabwensis OX=268475 GN=TY3B-I PE=4 SV=1) HSP 1 Score: 45.8246 bits (107), Expect = 3.611e-9 Identity = 26/61 (42.62%), Postives = 37/61 (60.66%), Query Frame = 3
Query: 321 PQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKN 503
P KC L E+ F GH V+ GI+T+ K E+V P + +R+FLGL +YYR F+K+
Sbjct: 722 PAKCRLCAKEILFSGHIVSRDGIRTDPSKTEKVASWPTPASTSEVRTFLGLASYYRRFVKS 782 HSP 2 Score: 44.669 bits (104), Expect = 3.611e-9 Identity = 38/122 (31.15%), Postives = 54/122 (44.26%), Query Frame = 1
Query: 550 ESFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVIA*RPT**PRXXXXXXXXXXXXXESCLW*KFIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYR 915
E+FQ L L IL R FI TDA+ I LSQ + + E VIA R + L +FI+RT H SL ++ K P + + WL++L EY+ + +R
Sbjct: 808 EAFQRLKRALTTAPILAFPRFDIPFIIDTDASETGIGAVLSQKHDPEGERVIAYE----SRTLSKTERKYSTTRKELLSIRFILRTDHDSLTWLGNFKEPEGQVAR-WLEHLQEYDMEVVHR 924
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A2G8LFI6 (Putative thy-1 membrane glycoprotein isoform X1 OS=Stichopus japonicus OX=307972 GN=BSL78_04104 PE=4 SV=1) HSP 1 Score: 53.1434 bits (126), Expect = 6.885e-9 Identity = 36/80 (45.00%), Postives = 49/80 (61.25%), Query Frame = 3
Query: 282 EKYFRRIKIEG*N--PQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKNCQKC 515
++ F+R++ G P KC L E+ +LGH V+ KGI T+ +K VEK E P+ +K +RSFLGL YYR FIK K
Sbjct: 300 DEVFQRLEKHGLKIKPSKCHLFQEEIGYLGHVVSSKGIATDPEKTAVVEKWEAPRNAKEVRSFLGLAGYYRRFIKGFSKI 379 HSP 2 Score: 36.5798 bits (83), Expect = 6.885e-9 Identity = 40/142 (28.17%), Postives = 58/142 (40.85%), Query Frame = 1
Query: 550 ESFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVIA-----*RPT**PRXXXXXXXXXXXXXESCLW------------*KFIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYRNRK 924
E+F+ L L +L ++ FI +TDA+ + LSQV D +E VIA RP N Y L + W +F + T H LV++ A + + + W LS YNF + YR K
Sbjct: 413 EAFEKLKKSLTTAPVLSYPDYTKPFIVHTDASAQGLGAVLSQV-SDGQEKVIAFASRTLRPA---EKNDSNYSAFKLELLALTWAVTEKFRDYLYGSQFTVYTDHNPLVYLKKAHLNTTEM--RWTARLSSYNFNIVYRPGK 548
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000051425.1 (pep primary_assembly:Astyanax_mexicanus-2.0:24:673219:676197:-1 gene:ENSAMXG00000029629.1 transcript:ENSAMXT00000051425.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 44.669 bits (104), Expect = 5.469e-9 Identity = 24/49 (48.98%), Postives = 35/49 (71.43%), Query Frame = 3
Query: 354 YFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIK 500
+FLGH V+G G++T+ KK E V P+ +K +RSF+GL +YYR FI+
Sbjct: 9 FFLGHVVSGAGVETDPKKTEAVRDWPTPRDAKMVRSFVGLASYYRRFIR 57 HSP 2 Score: 29.6462 bits (65), Expect = 5.469e-9 Identity = 21/52 (40.38%), Postives = 28/52 (53.85%), Query Frame = 1
Query: 553 SFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVIA 708
+F+ L RL IL + S FI TDA+ + LSQV +D E+VIA
Sbjct: 85 AFEELKSRLCNAPILAFPKVSESFIVDTDASDHGLGAVLSQV-QDGSEHVIA 135 HSP 3 Score: 24.2534 bits (51), Expect = 5.469e-9 Identity = 15/44 (34.09%), Postives = 24/44 (54.55%), Query Frame = 3
Query: 717 YLVTKIELVAVYEYFIHFRE-----LFVVKVYNSYLPQIISFYE 833
Y VT+ EL+AV E HFR F+++ ++ L ++ F E
Sbjct: 148 YCVTRRELIAVVESLKHFRAYMYGVPFLLRTGHALLQWLMRFRE 191
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000042044.1 (pep primary_assembly:Astyanax_mexicanus-2.0:19:20487577:20489571:-1 gene:ENSAMXG00000035832.1 transcript:ENSAMXT00000042044.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.2174 bits (121), Expect = 9.343e-9 Identity = 29/61 (47.54%), Postives = 41/61 (67.21%), Query Frame = 3
Query: 318 NPQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIK 500
NP KC L + FLGH V+G G++T+ KK E V VP+ +K +RSF+GL +YY+ FI+
Sbjct: 49 NPAKCNLLRQRVNFLGHVVSGAGVETDSKKTEAVRDWPVPRNAKMVRSFVGLASYYKRFIR 109 HSP 2 Score: 27.7202 bits (60), Expect = 9.343e-9 Identity = 39/135 (28.89%), Postives = 63/135 (46.67%), Query Frame = 1
Query: 553 SFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVIA---*RPT**PRXXXXXXXXXXXXXESCLW*K-------FIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYRNRKS 927
+F+ L RL +L S FI TDA+ + LSQV +D E VIA R R ++ +L ++ES + F++RT H SL ++ + P L + W+ L E++F + +R +S
Sbjct: 137 AFEELKSRLCNAPVLAYSNMSESFIVDTDASDRGLGAVLSQV-QDGSERVIAYYSRRLDKAERNYCVMRRELLAVVESLKHFRPYVYGIPFLLRTDHASLQWLMHFREPEGQLAR-WISRLQEFSFEVVHRPGRS 269
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000047000.1 (pep primary_assembly:Astyanax_mexicanus-2.0:21:9985706:9990085:1 gene:ENSAMXG00000032330.1 transcript:ENSAMXT00000047000.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 47.7506 bits (112), Expect = 2.011e-8 Identity = 29/62 (46.77%), Postives = 39/62 (62.90%), Query Frame = 3
Query: 318 NPQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKN 503
+P KC+ T + +LGH V+ G++T+ KVE ++ PK K LRSFLG YYR FIKN
Sbjct: 541 SPTKCKFFQTSVRYLGHIVSQDGVETDPNKVEALKTWPRPKGLKELRSFLGFSGYYRRFIKN 602 HSP 2 Score: 29.6462 bits (65), Expect = 2.011e-8 Identity = 38/148 (25.68%), Postives = 59/148 (39.86%), Query Frame = 1
Query: 517 EPFYCAESGYCE-SFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYV-IA*RPT**PRXXXXXXXXXXXXXESCLW*KF----------IIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYRNRK 924
+PF + CE SFQ + +L +L ++ +TDA+ + L Q +E K + A R + L+ + KF ++ S+ + TAKM +S WL LS +NF LKYR K
Sbjct: 638 QPFGSRWTPECECSFQAIIEKLTSAPVLGFANPQLPYVLHTDASTTGLGAALYQEQEGKMRVISYASRGLSRSESKYPAHKLEFLALKWAVTEKFNDYLYGSPFTVVTDSNPLTYILTTAKMDATSY--RWLATLSTFNFKLKYRAGK 783
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000030446.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000181.1:474500:475675:-1 gene:ENSAMXG00000036877.1 transcript:ENSAMXT00000030446.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 52.373 bits (124), Expect = 2.159e-8 Identity = 30/61 (49.18%), Postives = 41/61 (67.21%), Query Frame = 3
Query: 318 NPQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIK 500
NP KC L + FLGH V+G G++T+ KK E V VP+ +K +RSF+GL +YYR FI+
Sbjct: 49 NPAKCNLLRQRVNFLGHVVSGAGVETDPKKTEAVRDWPVPRNAKMVRSFVGLASYYRRFIR 109 HSP 2 Score: 25.0238 bits (53), Expect = 2.159e-8 Identity = 20/52 (38.46%), Postives = 25/52 (48.08%), Query Frame = 1
Query: 553 SFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVIA 708
+F L RL +L S FI TDA+ + LSQV +D E VIA
Sbjct: 137 AFGELKRRLCNAPVLAYPNMSESFIVDTDASDRGLGAVLSQV-QDGSERVIA 187
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000034038.1 (pep primary_assembly:Astyanax_mexicanus-2.0:14:3120146:3124090:1 gene:ENSAMXG00000040885.1 transcript:ENSAMXT00000034038.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 41.9726 bits (97), Expect = 2.391e-8 Identity = 26/60 (43.33%), Postives = 36/60 (60.00%), Query Frame = 3
Query: 321 PQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIK 500
PQKC L E+ +LGH V+ GI+T+ K+ +V +VP+ L FLG YYR FI+
Sbjct: 424 PQKCHLLRREVLYLGHVVSSDGIKTDPDKISKVAGWKVPENRHELLQFLGFAGYYRRFIE 483 HSP 2 Score: 35.039 bits (79), Expect = 2.391e-8 Identity = 38/144 (26.39%), Postives = 62/144 (43.06%), Query Frame = 1
Query: 520 PFYCAESGYCE-SFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYV-IA*R---------PT**PRXXXXXXXXXXXXXESCLW*KFIIRTSHKSLVF-MNTAKMPISSLFQTWLDNLSEYNFGLKYR 915
PF E CE +FQ L +L +L S+ F+ TDA+ + L+QV++ +E + A R P L + + KF + T + L + M TAK+ + Q W+ LS ++F ++YR
Sbjct: 516 PFIWTEE--CENAFQTLKTKLTTAPVLGYADYSQPFVLQTDASLAGLGAVLAQVQDGRERVIAYASRGLNPAETRYPAHKLEFLALKWAVTDKFYDHLYGHKFSVLTDNNPLKYVMTTAKLDATG--QRWVAQLSMFDFDIRYR 655
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000037906.1 (pep primary_assembly:ASM223467v1:16:28643737:28647831:-1 gene:ENSORLG00000027277.1 transcript:ENSORLT00000037906.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 49.2914 bits (116), Expect = 1.893e-7 Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 3
Query: 324 QKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKNCQK 512
QKC +E+ +LGH V+ G+ T+ K++ V + + P C K L+SFLG ++YR F+KN K
Sbjct: 539 QKCSFLKSEVAYLGHLVSAAGVATDPDKIKAVVEWKRPSCLKDLKSFLGFASFYRRFVKNFAK 601 HSP 2 Score: 25.0238 bits (53), Expect = 1.893e-7 Identity = 39/158 (24.68%), Postives = 61/158 (38.61%), Query Frame = 1
Query: 493 LLKTARNAEP---FYCAESGYCE-SFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQ-VKEDKEEYVIA*RPT**PRXXXXXXXXXXXXXESCLW------------*KFIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYR 915
LL T++ P F CE +FQ L +L +L + F+ DA+ ++ LSQ V A R N Y + L W KF I T + L +N+AK+ ++ Q W+ L+ ++F + YR
Sbjct: 612 LLSTSKGGRPTNGFQQLWDCVCEEAFQCLKKKLTSAPVLAYADFCKPFVLEIDASHQGLRAVLSQDVDGQLRPVAFASRGLRSSERNMENYSAMKLELLGLKWAVTEKFRDYLIGSKFTILTDNNPLCHLNSAKL--GAVEQRWVSELARFDFQICYR 767
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000031432.1 (pep primary_assembly:ASM223467v1:18:18329765:18334348:-1 gene:ENSORLG00000027440.1 transcript:ENSORLT00000031432.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 45.4394 bits (106), Expect = 2.822e-7 Identity = 29/86 (33.72%), Postives = 45/86 (52.33%), Query Frame = 3
Query: 282 EKYFRRIKIEG*N--PQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXX---------CSK*LRSFLGLINYYRSFIKNC 506
E F R++ G P+KC+L + FLGH V+ KG+ T+ KV+ + V K ++SFLG++ YY+ FI+ C
Sbjct: 563 EMVFGRLRAHGLKLAPKKCQLLRRSVKFLGHVVDSKGVSTDPDKVKAITAMTVMDLMMEDGITPSQKKIKSFLGMVMYYQQFIQGC 648 HSP 2 Score: 28.1054 bits (61), Expect = 2.822e-7 Identity = 18/47 (38.30%), Postives = 25/47 (53.19%), Query Frame = 1
Query: 544 YCESFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKE 684
+ +SF+ L L E IL +R FI TDA+ + LSQV+E
Sbjct: 687 HSDSFERLKSALLESVILAHPDFNRPFILSTDASTDGLGAVLSQVQE 733
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000034211.1 (pep primary_assembly:ASM223467v1:14:28948951:28953534:1 gene:ENSORLG00000029163.1 transcript:ENSORLT00000034211.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 45.4394 bits (106), Expect = 2.915e-7 Identity = 29/86 (33.72%), Postives = 45/86 (52.33%), Query Frame = 3
Query: 282 EKYFRRIKIEG*N--PQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXX---------XXCSK*LRSFLGLINYYRSFIKNC 506
E F R++ G P+KC+L + FLGH V+ KG+ T+ KV+ + V K ++SFLG++ YY+ FI+ C
Sbjct: 563 EMVFGRLRAHGLKLAPKKCQLLRRSVKFLGHVVDSKGVSTDPDKVKAITAMTVIDLMMEDGITPSQKKIKSFLGMVMYYQQFIQGC 648 HSP 2 Score: 28.1054 bits (61), Expect = 2.915e-7 Identity = 18/47 (38.30%), Postives = 25/47 (53.19%), Query Frame = 1
Query: 544 YCESFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKE 684
+ +SF+ L L E IL +R FI TDA+ + LSQV+E
Sbjct: 687 HSDSFERLKSALLESVILAHPDFNRPFILSTDASTDGLGAVLSQVQE 733
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000029819.1 (pep primary_assembly:ASM223467v1:24:6358775:6362914:-1 gene:ENSORLG00000022361.1 transcript:ENSORLT00000029819.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 38.891 bits (89), Expect = 3.579e-7 Identity = 27/79 (34.18%), Postives = 44/79 (55.70%), Query Frame = 3
Query: 282 EKYFRRIKIEG*NPQ--KCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKNCQK 512
E R+++EG + KC ++++LGH ++ +G+ T+ KVE V E P LRSF+G +YYR F++ K
Sbjct: 518 ELVLERLQLEGLKVKLAKCAFFQHQVHYLGHVISDQGVSTDPGKVEAVANWEPPTTVFQLRSFIGFASYYRRFVEGFAK 596 HSP 2 Score: 34.2686 bits (77), Expect = 3.579e-7 Identity = 42/159 (26.42%), Postives = 68/159 (42.77%), Query Frame = 1
Query: 499 KTARNAEPFYCAESGYCE-SFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVI----A*RPT**PR-----------XXXXXXXXXXXXXESCLW*KFIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYRNRKS 927
K+AR + E C+ SF+ L +L +L SR FI DA+ G + LSQ +E K + RPT R L + E L K I+ T + L +++TAK+ ++ Q W L+ ++ ++YR+ +S
Sbjct: 616 KSARGLTNHWTEE---CQRSFEALKAKLTTTPVLAYADFSRPFILEVDASNGGLGAVLSQEQEGKVRPIAYASRGLRPT--ERNPVNYSSMRLEFLALKWAVAEKFREYLLGQKCIVYTDNNPLSYLSTAKL--GAMEQRWAAQLAAFDLEIRYRSGRS 767
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000039099.1 (pep primary_assembly:ASM223467v1:16:31928608:31932747:-1 gene:ENSORLG00000023909.1 transcript:ENSORLT00000039099.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 38.891 bits (89), Expect = 3.579e-7 Identity = 27/79 (34.18%), Postives = 44/79 (55.70%), Query Frame = 3
Query: 282 EKYFRRIKIEG*NPQ--KCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKNCQK 512
E R+++EG + KC ++++LGH ++ +G+ T+ KVE V E P LRSF+G +YYR F++ K
Sbjct: 518 ELVLERLQLEGLKVKLAKCAFFQHQVHYLGHVISDQGVSTDPGKVEAVANWEPPTTVFQLRSFIGFASYYRRFVEGFAK 596 HSP 2 Score: 34.2686 bits (77), Expect = 3.579e-7 Identity = 42/159 (26.42%), Postives = 68/159 (42.77%), Query Frame = 1
Query: 499 KTARNAEPFYCAESGYCE-SFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVI----A*RPT**PR-----------XXXXXXXXXXXXXESCLW*KFIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYRNRKS 927
K+AR + E C+ SF+ L +L +L SR FI DA+ G + LSQ +E K + RPT R L + E L K I+ T + L +++TAK+ ++ Q W L+ ++ ++YR+ +S
Sbjct: 616 KSARGLTNHWTEE---CQRSFEALKAKLTTTPVLAYADFSRPFILEVDASNGGLGAVLSQEQEGKVRPIAYASRGLRPT--ERNPVNYSSMRLEFLALKWAVAEKFREYLLGQKCIVYTDNNPLSYLSTAKL--GAMEQRWAAQLAAFDLEIRYRSGRS 767
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000045297.1 (SMESG000045297.1) HSP 1 Score: 79.7221 bits (195), Expect = 1.045e-27 Identity = 56/150 (37.33%), Postives = 79/150 (52.67%), Query Frame = 1
Query: 505 ARNAEPFYCAESGYCESFQ----------VLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVIA*RPT**PRXXXXXXXXXXXXXESCLW*KFII---RTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYR 915
A A P Y A SG +S Q L ++L + ILD RK+ +F+ DA+FG I + LSQ+K++ +E V+A + +I C+ K ++ T HK +FMNT K PIS FQTW+ NLSEY+F L+YR
Sbjct: 179 AEIAAPLYAATSGCDKSLQWTDRCGKCFNELKMKLCKAPILDYPRKNLMFVLDIDASFGVIDLVLSQIKKNGDEAVVA----------YGSWHLSSHIKIYCVTRKELLALHETDHKVFIFMNTTKKPISPKFQTWMANLSEYDFVLQYR 318 HSP 2 Score: 62.7734 bits (151), Expect = 1.045e-27 Identity = 41/68 (60.29%), Postives = 50/68 (73.53%), Query Frame = 3
Query: 297 RIKIEG*NPQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIK 500
RIK E P+KC+ EL FLGHT++ +GI+TN K+EE+ K+E PKCS LRSFLGL NYYR FIK
Sbjct: 112 RIKSE---PKKCKFYQKELTFLGHTISSEGIRTNNLKIEEILKAEAPKCSSKLRSFLGLANYYRRFIK 176
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000064819.1 (SMESG000064819.1) HSP 1 Score: 75.0998 bits (183), Expect = 5.569e-25 Identity = 55/144 (38.19%), Postives = 75/144 (52.08%), Query Frame = 1
Query: 514 AEPFYCAESG---------YC-ESFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVIA*RPT**PRXXXXXXXXXXXXXESCLW*KFIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYR 915
A P Y A +G C + F L L E IL+ R R+FI TDA+FG I+ LSQ+KED E VIA + L+ I++ +F+ RT +K+L FMNT PIS F+T + NL+EY+F L+ R
Sbjct: 110 AAPLYAAMTGNDKMIVWTEECRKCFINLKKLLCEAPILEYPRADRLFIIDTDASFGAIRTVLSQIKEDGTEGVIA-------KELLALHEFIVHFRHYLYGRRFVARTDNKALTFMNTTIKPISPQFRTRMANLTEYDFALQCR 246 HSP 2 Score: 58.151 bits (139), Expect = 5.569e-25 Identity = 36/62 (58.06%), Postives = 46/62 (74.19%), Query Frame = 3
Query: 318 NPQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKN 503
NP+KC L+ F+GHTV+ KGIQTN++K+ E+E + PK + LRSFLGL NYYR FIKN
Sbjct: 49 NPEKCVLN-----FIGHTVSEKGIQTNKRKISEIENATKPKSTTELRSFLGLTNYYRRFIKN 105
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000080555.1 (SMESG000080555.1) HSP 1 Score: 70.0922 bits (170), Expect = 5.843e-23 Identity = 55/148 (37.16%), Postives = 67/148 (45.27%), Query Frame = 1
Query: 514 AEPFYCAESGYCESFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVIA*RPT**PRXXXXXXXXXXXXXESCL--------------W*KFIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYR 915
A P Y +G + V E IL+ R RIFI TDA+F I LSQ+KED E VIA R M + L W +F+ T HK+L FMNT K FQTW+ NLSEY+F L+YR
Sbjct: 121 AAPLYATTTGNDKMI----VWTEESPILEYHRADRIFIIDTDASFEAIGSVLSQIKEDGTEGVIAYGS----RHLTKHEMGYCVTRKELLALHEFIVHFRQYLYWRRFVDTTDHKALTFMNTTKNQFPQ-FQTWMANLSEYDFALQYR 259 HSP 2 Score: 56.225 bits (134), Expect = 5.843e-23 Identity = 33/62 (53.23%), Postives = 46/62 (74.19%), Query Frame = 3
Query: 318 NPQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKN 503
NP+K + + L F+GHTV+ KGI++N++K+ E+E + PK + LRSFLGL NYYR FIKN
Sbjct: 55 NPEKYAFNTSILNFIGHTVSEKGIRSNKRKISEIENATEPKSTTELRSFLGLTNYYRRFIKN 116
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000009248.1 (SMESG000009248.1) HSP 1 Score: 64.6994 bits (156), Expect = 1.661e-22 Identity = 43/55 (78.18%), Postives = 50/55 (90.91%), Query Frame = 3
Query: 339 SNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKN 503
+N +L F+GHTVNG+GIQTNRKKVEE+EK+EVPKCSK LRSFLGL NYYR FIK+
Sbjct: 94 TNYKLCFIGHTVNGEGIQTNRKKVEEIEKAEVPKCSKQLRSFLGLTNYYRRFIKD 148 HSP 2 Score: 60.077 bits (144), Expect = 1.661e-22 Identity = 38/71 (53.52%), Postives = 40/71 (56.34%), Query Frame = 1
Query: 505 ARNAEPFYCAESG---------YC-ESFQVLGVRLREGFILDLLRKSRIFIFYTDANFGDIKVFLSQVKED 687
R AEP Y A SG C ESFQVL RL E ILD R+ R FI TDA+FG I LSQVKED
Sbjct: 150 TRIAEPLYAATSGCDKDIIWTKECNESFQVLKGRLCEALILDYPREGRTFILDTDASFGAIGTVLSQVKED 220
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000081257.1 (SMESG000081257.1) HSP 1 Score: 63.5438 bits (153), Expect = 1.696e-22 Identity = 49/146 (33.56%), Postives = 68/146 (46.58%), Query Frame = 1
Query: 514 AEPFYCAESGYCESFQVLGVRLREGFI-----------LDLLRKSRIFIFYTDANFGDIKVFLSQVKEDKEEYVIA*RPT**PRXXXXXXXXXXXXXESCLW*K-FIIRTSHKSLVFMNTAKMPISSLFQTWLDNLSEYNFGLKYR 915
A P Y A +G C+ V R+ FI L+ R R+FI TD +F I + + + Y + R L+ + L+ + F+ RT HK+L FMNT K PIS FQTW+ N SEY+F L+YR
Sbjct: 703 AAPLYAATTG-CDKMIVWTEECRKRFINLKKLLCEAPILEYPRADRLFIIDTDDSFEAI----GHLTKHEIGYCVT-------RKELLVLHEFIVHFRQYLYGRRFVARTDHKALTFMNTTKKPISPQFQTWMANFSEYDFALQYR 836 HSP 2 Score: 61.2326 bits (147), Expect = 1.696e-22 Identity = 39/75 (52.00%), Postives = 52/75 (69.33%), Query Frame = 3
Query: 285 KYFRRIKIEG*--NPQKCELSNTELYFLGHTVNGKGIQTNRXXXXXXXXXXXXXCSK*LRSFLGLINYYRSFIKN 503
+ F RI+ G NP+KC + + L F+GHTV+ KGIQTN++K+ E+E + PK + LRSFLGL YYR FIKN
Sbjct: 624 EVFTRIRDSGLKVNPEKCAFNKSVLNFIGHTVSEKGIQTNKRKISEIENATEPKSTTELRSFLGLTTYYRRFIKN 698 The following BLAST results are available for this feature:
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30003181 ID=SMED30003181|Name=Reverse transcriptase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1071bpback to top Annotated Terms
The following terms have been associated with this transcript:
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