SMED30003142

Overview
NameSMED30003142
Smed IDSMED30003142
Length (bp)1884
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003142 (SMED30003142) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003142 (SMED30003142) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003142 (SMED30003142) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003142 (SMED30003142) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003142

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30003142h1SMcG0018230 dd_Smed_v4_10003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30003142h1SMcG0018230 dd_Smed_v4_10003_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003142 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002032:18196..42276 +8947
Homology
BLAST of SMED30003142 vs. Ensembl Human
Match: TMX3 (thioredoxin related transmembrane protein 3 [Source:HGNC Symbol;Acc:HGNC:24718])

HSP 1 Score: 172.555 bits (436), Expect = 9.316e-47
Identity = 120/425 (28.24%), Postives = 206/425 (48.47%), Query Frame = -1
Query:  397 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESST--MKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEAR 1659
            V +L+ +F E  +   WLV FYAPWCG+C+KLEPI+NE+   +K +  P+K+ ++DAT Y  +AS + +RGYPTIK + G   Y + G RT + +I F+ R  G  ++ LPS+  F    + H    FF+Y+G  S  LK  Y D A+ L + +YF+S   + +P+  Y  L         E   +LV+K +    +   E     +  L +WI+  RF  +  +    L+                   +      +   E+ +S    +L S  +++A +  +    D  F        +N L M  L VP V++    N   ++       +++K +++  +  F+++   G +E  GG     +++R  +D  + ++    ++P++  F+ G+PL VIS +CY I    T+D D   IE R
Sbjct:   28 VEDLDESFKENRNDDIWLVDFYAPWCGHCKKLEPIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIKLLKGDLAYNYRGPRTKDDIIEFAHRVSGALIRPLPSQQMFEHMQKRH--RVFFVYVGGESP-LKEKYIDAASELIVYTYFFSASEEVVPE--YVTL--------KEMPAVLVFKDETYFVYDEYE-----DGDLSSWINRERFQNYLAMDGFLLY---------------ELGDTGKLVALAVIDEKNTSVEHTRLKSIIQEVARDYRDLFHRDFQFGHMDGNDYINTLLMDELTVPTVVVLNTSNQQYFLLD-----RQIKNVED--MVQFINNILDGTVEAQGGDSILQRLKRIVFDAKSTIVSIFKSSPLMGCFLFGLPLGVISIMCYGIY---TADTDGGYIEER 409          
BLAST of SMED30003142 vs. Ensembl Human
Match: TMX3 (thioredoxin related transmembrane protein 3 [Source:HGNC Symbol;Acc:HGNC:24718])

HSP 1 Score: 133.265 bits (334), Expect = 3.014e-35
Identity = 67/166 (40.36%), Postives = 102/166 (61.45%), Query Frame = -1
Query: 1168 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQ 1659
            V +L+ +F E  +   WLV FYAPWCG+C+KLEPI+NE+   +K +  P+K+ ++DAT Y  +AS + +RGYPTIK + G   Y + G RT + +I F+ R  G  ++ LPS+  F    + H    FF+Y+G  S  LK  Y D A+ L + +YF+S   + +P+
Sbjct:   28 VEDLDESFKENRNDDIWLVDFYAPWCGHCKKLEPIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIKLLKGDLAYNYRGPRTKDDIIEFAHRVSGALIRPLPSQQMFEHMQKRH--RVFFVYVGGESP-LKEKYIDAASELIVYTYFFSASEEVVPE 190          
BLAST of SMED30003142 vs. Ensembl Human
Match: TMX3 (thioredoxin related transmembrane protein 3 [Source:HGNC Symbol;Acc:HGNC:24718])

HSP 1 Score: 103.219 bits (256), Expect = 1.508e-24
Identity = 58/166 (34.94%), Postives = 88/166 (53.01%), Query Frame = -1
Query: 1168 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQ 1659
            V +L+ +F E  +   WLV FYAPWCG+C+KLEPI+NE+   +K +  P+K+ ++DAT Y  +AS + +RGYPTIK                              ++ LPS+  F    + H    FF+Y+G  S  LK  Y D A+ L + +YF+S   + +P+
Sbjct:   28 VEDLDESFKENRNDDIWLVDFYAPWCGHCKKLEPIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIKL---------------------------ALIRPLPSQQMFEHMQKRH--RVFFVYVGGESP-LKEKYIDAASELIVYTYFFSASEEVVPE 163          
BLAST of SMED30003142 vs. Ensembl Human
Match: TMX3 (thioredoxin related transmembrane protein 3 [Source:HGNC Symbol;Acc:HGNC:24718])

HSP 1 Score: 85.8853 bits (211), Expect = 2.462e-19
Identity = 38/76 (50.00%), Postives = 55/76 (72.37%), Query Frame = -1
Query: 1438 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIK 1659
            V +L+ +F E  +   WLV FYAPWCG+C+KLEPI+NE+   +K +  P+K+ ++DAT Y  +AS + +RGYPTIK
Sbjct:   28 VEDLDESFKENRNDDIWLVDFYAPWCGHCKKLEPIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIK 103          
BLAST of SMED30003142 vs. Ensembl Human
Match: P4HB (prolyl 4-hydroxylase subunit beta [Source:HGNC Symbol;Acc:HGNC:8548])

HSP 1 Score: 82.0333 bits (201), Expect = 8.084e-16
Identity = 71/233 (30.47%), Postives = 111/233 (47.64%), Query Frame = -1
Query:  991 NSNFIE-IADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIK--DLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYT---HDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRF--------STFPKI 1647
             SNF E +A     LV+FYAPWCG+C+ L P Y +    +K     I++A++DAT   D+A  Y +RGYPTIK+    D  +   +   R  + ++ + K+  GPA   LP       A ES +  +    IG    + K V  D A      +   +I++  IP    S+ D+ S     ++G +L  K D   + +F+ E++  N  L ++I  N+          T PKI
Sbjct:   31 KSNFAEALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKLKAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFFRNGDTASPKEYTAGREADDIVNWLKKRTGPAATTLPDGA----AAESLVESSEVAVIG----FFKDVESDSAKQFLQAA--EAIDD--IPFGITSNSDVFSKYQLDKDGVVLFKKFDEGRN-NFEGEVTKEN--LLDFIKHNQLPLVIEFTEQTAPKI 248          

HSP 2 Score: 55.4546 bits (132), Expect = 2.009e-7
Identity = 31/99 (31.31%), Postives = 58/99 (58.59%), Query Frame = -1
Query: 1372 LNSNFIEIA--DKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDATRYPDVASFYQIRGYPTIKYINGT---DIYTHDGDRTVEHLILF 1650
            +  NF ++A  +K    V+FYAPWCG+C++L PI+++L    KD   I IA++D+T   +     ++  +PT+K+   +    +  ++G+RT++    F
Sbjct:  373 VGKNFEDVAFDEKKNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENIVIAKMDST--ANEVEAVKVHSFPTLKFFPASADRTVIDYNGERTLDGFKKF 469          
BLAST of SMED30003142 vs. Ensembl Celegans
Match: ZK973.11 (pep chromosome:WBcel235:I:4379325:4381948:-1 gene:WBGene00022836.1 transcript:ZK973.11.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ZK973.11)

HSP 1 Score: 135.191 bits (339), Expect = 5.633e-34
Identity = 101/422 (23.93%), Postives = 201/422 (47.63%), Query Frame = -1
Query:  418 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKD--LPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFN---DEQNDLFFVW--STDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMD-TSDAD 1659
            V++L+  F+++ D+G W V+FYAPWC +C++L P+++++   + D  LPI++ ++D TR+P VA+   I+GYPTI +     +  + G R  E L+ F+KR   P ++ + +E +  +   S   +  +++ G  S  L   +++ A+S    + FYS+       S+                 + V+K + ++ F+   E       L  W++  R+  F + +  +L E+    K +++++ + S                +S ++ F    + A +      D  N   F W   +D+AS   + M  +  P++ IF   ++  Y+  D  +   +K+I   F+           I  +GG     +++R  ++    + +  +T P+L+  + GVP++ +S +CY+IC  D T D D
Sbjct:   30 VLDLSDKFLDVKDEGMWFVEFYAPWCAHCKRLHPVWDQVGHTLSDSNLPIRVGKLDCTRFPAVANKLSIQGYPTILFFRNGHVIDYRGGREKEALVSFAKRCAAPIIEVI-NENQIEKVKLSARSQPSYVFFGTSSGPLFDAFNEAASSKFSVARFYSVAPPENDASFRQR--------------VAVFKDNFEIEFNGDIE------KLTEWVTRERWPGFLQATSSNLAEIGASGKLVVLVVSSESHKF-----------NNTSPIREFHKTAEEASKELRKHPDLWNRFQFAWLDGSDLASQ--IQMAAVSEPHLFIFNYTSYEYYLSEDEPSQMTIKSI-LTFLEQTSEGIDKETIVAFGGRHLLTRIKRMAFELYWNIAQMFATQPLLSSCLFGVPIAFLSIICYSICSADFTVDRD 416          
BLAST of SMED30003142 vs. Ensembl Celegans
Match: pdi-2 (Protein disulfide-isomerase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q17770])

HSP 1 Score: 85.8853 bits (211), Expect = 1.757e-17
Identity = 65/231 (28.14%), Postives = 109/231 (47.19%), Query Frame = -1
Query:  982 NVIELNS-NFIEIADKGPW-LVKFYAPWCGYCRKLEPIYNELEAHIKDLP--IKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQH 1662
            NVI L   NF E+ +   + LV+FYAPWCG+C+ L P Y +    +K+    IK+ ++DAT + +V+S +++RGYPT+K         ++G R  + +I + K+  GP  K L       E  ES  +     Y  D +S     + ++A  +    +  S E+     +  S ++L         G +L  K D      F E+++     L+ WI  NR +   + +Q 
Sbjct:   24 NVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADV-VVIGYFKDTTSDDAKTFLEVAAGIDDVPFGISTED-----AVKSEIELKGE------GIVLFKKFDDG-RVAFDEKLT--QDGLKTWIQANRLALVSEFTQE 239          

HSP 2 Score: 61.6178 bits (148), Expect = 1.054e-9
Identity = 33/84 (39.29%), Postives = 50/84 (59.52%), Query Frame = -1
Query: 1372 LVKFYAPWCGYCRKLEPIYNEL-EAHIKDLPIKIARIDAT--RYPDVASFYQIRGYPTIKYI--NGTDIYTHDGDRTVEHLILF 1608
            LV+FYAPWCG+C++L P +++L E    D  I IA++D+T     DV    +I+ +PTIK+       +  + GDRT+E    F
Sbjct:  385 LVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDV----KIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKF 464          
BLAST of SMED30003142 vs. Ensembl Celegans
Match: pdi-2 (Protein disulfide-isomerase 2 [Source:UniProtKB/Swiss-Prot;Acc:Q17770])

HSP 1 Score: 85.8853 bits (211), Expect = 1.757e-17
Identity = 65/231 (28.14%), Postives = 109/231 (47.19%), Query Frame = -1
Query:  982 NVIELNS-NFIEIADKGPW-LVKFYAPWCGYCRKLEPIYNELEAHIKDLP--IKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQH 1662
            NVI L   NF E+ +   + LV+FYAPWCG+C+ L P Y +    +K+    IK+ ++DAT + +V+S +++RGYPT+K         ++G R  + +I + K+  GP  K L       E  ES  +     Y  D +S     + ++A  +    +  S E+     +  S ++L         G +L  K D      F E+++     L+ WI  NR +   + +Q 
Sbjct:   24 NVIVLTKDNFDEVINGNEFILVEFYAPWCGHCKSLAPEYAKAATQLKEEGSDIKLGKLDATVHGEVSSKFEVRGYPTLKLFRNGKPQEYNGGRDHDSIIAWLKKKTGPVAKPLADADAVKELQESADV-VVIGYFKDTTSDDAKTFLEVAAGIDDVPFGISTED-----AVKSEIELKGE------GIVLFKKFDDG-RVAFDEKLT--QDGLKTWIQANRLALVSEFTQE 239          

HSP 2 Score: 61.6178 bits (148), Expect = 1.054e-9
Identity = 33/84 (39.29%), Postives = 50/84 (59.52%), Query Frame = -1
Query: 1372 LVKFYAPWCGYCRKLEPIYNEL-EAHIKDLPIKIARIDAT--RYPDVASFYQIRGYPTIKYI--NGTDIYTHDGDRTVEHLILF 1608
            LV+FYAPWCG+C++L P +++L E    D  I IA++D+T     DV    +I+ +PTIK+       +  + GDRT+E    F
Sbjct:  385 LVEFYAPWCGHCKQLAPTWDKLGEKFADDESIVIAKMDSTLNEVEDV----KIQSFPTIKFFPAGSNKVVDYTGDRTIEGFTKF 464          
BLAST of SMED30003142 vs. Ensembl Celegans
Match: pdi-1 (Protein disulfide-isomerase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q17967])

HSP 1 Score: 85.8853 bits (211), Expect = 2.043e-17
Identity = 51/153 (33.33%), Postives = 81/153 (52.94%), Query Frame = -1
Query: 1216 NVIELN-SNFIEIADKGPW-LVKFYAPWCGYCRKLEPIYNELEAHIKDLP--IKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSL 1662
            NV+ L  SNF E  +   + LVKFYAPWC +C+ L P Y+E    +K+    IK+A++DAT    +AS +++RGYPTI Y        + G R    ++ + K+  GP V  + S  +  E ++         Y  D  S    +Y+++A+S+
Sbjct:   24 NVLVLTESNFEETINGNEFVLVKFYAPWCVHCKSLAPKYDEAADLLKEEGSDIKLAKVDATENQALASKFEVRGYPTILYFKSGKPTKYTGGRATAQIVDWVKKKSGPTVTTVESVEQLEE-LKGKTRVVVLGYFKDAKSDAATIYNEVADSV 175          

HSP 2 Score: 63.1586 bits (152), Expect = 2.879e-10
Identity = 39/108 (36.11%), Postives = 59/108 (54.63%), Query Frame = -1
Query: 1348 LNSNFIEIA--DKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDAT--RYPDVASFYQIRGYPTIKY--INGTDIYTHDGDRTVEHLILFSKRALGPA 1650
            + SNF EIA  +     VKFYAPWCG+C++L P+++EL    +  P + IA++DAT     DV    ++  +PT+K      +    +DGDR +E    F  +  G A
Sbjct:  369 VASNFNEIALDETKTVFVKFYAPWCGHCKQLVPVWDELAEKYESNPNVVIAKLDATLNELADV----KVNSFPTLKLWPAGSSTPVDYDGDRNLEKFEEFVNKYAGSA 472          
BLAST of SMED30003142 vs. Ensembl Celegans
Match: pdi-1 (Protein disulfide-isomerase 1 [Source:UniProtKB/Swiss-Prot;Acc:Q17967])

HSP 1 Score: 85.8853 bits (211), Expect = 2.043e-17
Identity = 51/153 (33.33%), Postives = 81/153 (52.94%), Query Frame = -1
Query: 1216 NVIELN-SNFIEIADKGPW-LVKFYAPWCGYCRKLEPIYNELEAHIKDLP--IKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSL 1662
            NV+ L  SNF E  +   + LVKFYAPWC +C+ L P Y+E    +K+    IK+A++DAT    +AS +++RGYPTI Y        + G R    ++ + K+  GP V  + S  +  E ++         Y  D  S    +Y+++A+S+
Sbjct:   24 NVLVLTESNFEETINGNEFVLVKFYAPWCVHCKSLAPKYDEAADLLKEEGSDIKLAKVDATENQALASKFEVRGYPTILYFKSGKPTKYTGGRATAQIVDWVKKKSGPTVTTVESVEQLEE-LKGKTRVVVLGYFKDAKSDAATIYNEVADSV 175          

HSP 2 Score: 63.1586 bits (152), Expect = 2.879e-10
Identity = 39/108 (36.11%), Postives = 59/108 (54.63%), Query Frame = -1
Query: 1348 LNSNFIEIA--DKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDAT--RYPDVASFYQIRGYPTIKY--INGTDIYTHDGDRTVEHLILFSKRALGPA 1650
            + SNF EIA  +     VKFYAPWCG+C++L P+++EL    +  P + IA++DAT     DV    ++  +PT+K      +    +DGDR +E    F  +  G A
Sbjct:  369 VASNFNEIALDETKTVFVKFYAPWCGHCKQLVPVWDELAEKYESNPNVVIAKLDATLNELADV----KVNSFPTLKLWPAGSSTPVDYDGDRNLEKFEEFVNKYAGSA 472          
BLAST of SMED30003142 vs. Ensembl Fly
Match: CG5027 (gene:FBgn0036579 transcript:FBtr0075457)

HSP 1 Score: 149.058 bits (375), Expect = 7.887e-39
Identity = 105/431 (24.36%), Postives = 200/431 (46.40%), Query Frame = -1
Query:  385 NVIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSF---HFKEEI--SSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEARQQKK 1662
             V+EL+  FI++  +G WLV FYAPWCGYC+K EPI+  +   +    +++ R+D T+YP  A  +++RGYPTI +I G   +T++GDR  + L+ ++ R  GP V+ +           SH +  FF+++G +   +   Y   A   +   +FY+       Q    H D     +      ++V+K ++   +   H   E+  + +N ++  W++  RF+ FPK+++ ++      +          +++ +   N     E E   M      K  A   ++D+     F W  + +  + + +  L  P+++         +I P+ +  Q    +  + +H FL S ++     YGG  +  ++ RA ++    L +     P+L   I G+PL  +S + Y+I C D    +    E  ++K+
Sbjct:   27 KVLELSDRFIDVRHEGQWLVMFYAPWCGYCKKTEPIFALVAQALHATNVRVGRLDCTKYPAAAKEFKVRGYPTIMFIKGNMEFTYNGDRGRDELVDYALRMSGPPVQLVTRTESVDMLKGSHTI--FFIFVGQQEGVVWDTYYAAAEGYQEHGFFYATSEDIAAQ----HFDFEKLPA------VIVYKEEQHHFYPHGHLAHEMDPNEVNETVFQWVNVERFTLFPKVTRFNI-----HQLLKTNKYLVLAVVQEDKLNQIATHELEFRDMVEGVIRKHRA--RYHDK---FQFGWIGEPSIAHSIILDQLPTPHLIAINSSTQHHFI-PEDDPMQ----MTPQALHLFLESIRNESAIAYGGDTYFVRLNRALFEVRRALRDMWLGNPVLTTVIFGLPLGFLSLIMYSIFCGDCLVTEEDPDEDHEKKE 430          
BLAST of SMED30003142 vs. Ensembl Fly
Match: Pdi (gene:FBgn0286818 transcript:FBtr0346644)

HSP 1 Score: 87.4261 bits (215), Expect = 8.972e-18
Identity = 62/217 (28.57%), Postives = 102/217 (47.00%), Query Frame = -1
Query:  991 IADKGPWLVKFYAPWCGYCRKLEPIYNELEAHI--KDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKP--DRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKI 1629
            IAD    LV+FYAPWCG+C+ L P Y +    +  K+ PIK+A++DAT   ++A  Y +RGYPT+K+        + G R    +I +  +  GP  K L S     + ++ + +     +  D  S     +  +AN+  L S+ + +          S+ D+ +     +NG +L +KP  D+K  F        LN   +N   F +  + P I
Sbjct:   41 IADNEFVLVEFYAPWCGHCKALAPEYAKAAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSGSPVEYSGGRQAADIIAWVTKKTGPPAKDLTSVADAEQFLKDNEI-AIIGFFKDLESEEAKTFTKVANA--LDSFVFGVS---------SNADVIAKYEAKDNGVVL-FKPFDDKKSVFE-----GELNE--ENLKKFAQVQSLPLI 237          

HSP 2 Score: 64.3142 bits (155), Expect = 1.750e-10
Identity = 39/98 (39.80%), Postives = 59/98 (60.20%), Query Frame = -1
Query: 1372 LNSNFIEIA-DKGP-WLVKFYAPWCGYCRKLEPIYNELEAHIKD-LPIKIARIDATRYPDVASFYQIRGYPTIKYINGTD--IYTHDGDRTVEHLILF 1650
            ++SNF  +A DK    LV+FYAPWCG+C++L PIY++L    KD   I IA++D+T   +     +I  +PTIKY    D  +   + DRT++  + F
Sbjct:  373 VSSNFESVALDKSKSVLVEFYAPWCGHCKQLAPIYDQLAEKYKDNEDIVIAKMDST--ANELESIKISSFPTIKYFRKEDNKVIDFNLDRTLDDFVKF 468          
BLAST of SMED30003142 vs. Ensembl Fly
Match: Pdi (gene:FBgn0286818 transcript:FBtr0075648)

HSP 1 Score: 87.4261 bits (215), Expect = 8.972e-18
Identity = 62/217 (28.57%), Postives = 102/217 (47.00%), Query Frame = -1
Query:  991 IADKGPWLVKFYAPWCGYCRKLEPIYNELEAHI--KDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKP--DRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKI 1629
            IAD    LV+FYAPWCG+C+ L P Y +    +  K+ PIK+A++DAT   ++A  Y +RGYPT+K+        + G R    +I +  +  GP  K L S     + ++ + +     +  D  S     +  +AN+  L S+ + +          S+ D+ +     +NG +L +KP  D+K  F        LN   +N   F +  + P I
Sbjct:   41 IADNEFVLVEFYAPWCGHCKALAPEYAKAAQQLAEKESPIKLAKVDATVEGELAEQYAVRGYPTLKFFRSGSPVEYSGGRQAADIIAWVTKKTGPPAKDLTSVADAEQFLKDNEI-AIIGFFKDLESEEAKTFTKVANA--LDSFVFGVS---------SNADVIAKYEAKDNGVVL-FKPFDDKKSVFE-----GELNE--ENLKKFAQVQSLPLI 237          

HSP 2 Score: 64.3142 bits (155), Expect = 1.750e-10
Identity = 39/98 (39.80%), Postives = 59/98 (60.20%), Query Frame = -1
Query: 1372 LNSNFIEIA-DKGP-WLVKFYAPWCGYCRKLEPIYNELEAHIKD-LPIKIARIDATRYPDVASFYQIRGYPTIKYINGTD--IYTHDGDRTVEHLILF 1650
            ++SNF  +A DK    LV+FYAPWCG+C++L PIY++L    KD   I IA++D+T   +     +I  +PTIKY    D  +   + DRT++  + F
Sbjct:  373 VSSNFESVALDKSKSVLVEFYAPWCGHCKQLAPIYDQLAEKYKDNEDIVIAKMDST--ANELESIKISSFPTIKYFRKEDNKVIDFNLDRTLDDFVKF 468          
BLAST of SMED30003142 vs. Ensembl Fly
Match: ERp60 (gene:FBgn0033663 transcript:FBtr0088058)

HSP 1 Score: 76.2554 bits (186), Expect = 2.707e-14
Identity = 43/141 (30.50%), Postives = 73/141 (51.77%), Query Frame = -1
Query: 1198 LVKFYAPWCGYCRKLEPIYNELEAHIK--DLPIKIARIDATRY-PDVASFYQIRGYPTIKYINGTDI-YTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYF 1608
            LV FYAPWCG+C++L+P Y +    +K  D PIK+A++D T    +  S Y + GYPT+K     ++   ++G R    +  + +  +GPA K + +     + +++    T F Y  D  S L  ++   A+  R +  F
Sbjct:   43 LVMFYAPWCGHCKRLKPEYAKAAEIVKDDDPPIKLAKVDCTEAGKETCSKYSVSGYPTLKIFRQDEVSQDYNGPREASGIAKYMRAQVGPASKTVRTVAELKKFLDTKDT-TLFGYFSDSDSKLAKIFLKFADKNREKYRF 182          

HSP 2 Score: 67.781 bits (164), Expect = 1.308e-11
Identity = 32/99 (32.32%), Postives = 56/99 (56.57%), Query Frame = -1
Query: 1321 LVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYI---NGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGR 1608
            L++FYAPWCG+C+KL PIY EL   ++D  + I ++DAT   DV   + +RG+PT+ ++         +++G R V+  + +  +     +K     G+
Sbjct:  386 LIEFYAPWCGHCKKLSPIYEELAEKLQDEDVAIVKMDATAN-DVPPEFNVRGFPTLFWLPKDAKNKPVSYNGGREVDDFLKYIAKEATTELKGFDRSGK 483          
BLAST of SMED30003142 vs. Ensembl Fly
Match: CG9302 (gene:FBgn0032514 transcript:FBtr0080470)

HSP 1 Score: 73.9442 bits (180), Expect = 1.543e-13
Identity = 37/101 (36.63%), Postives = 61/101 (60.40%), Query Frame = -1
Query: 1381 VIELNSNFIEIA--DKGPWLVKFYAPWCGYCRKLEPIYNE--LEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYI-NGT---DIYTHDGDRTVEHL 1659
            ++ L S   E A  D+   LV FYAPWCG+C++++P Y +  LE   K +P  +A +DAT+ P +A  Y+++GYPT+K+  NG    ++   +  + VE +
Sbjct:  273 IVHLTSQGFEPALKDEKSALVMFYAPWCGHCKRMKPEYEKAALEMKQKKIPGLLAALDATKEPSIAEKYKVKGYPTVKFFSNGVFKFEVNVREASKIVEFM 373          

HSP 2 Score: 59.3066 bits (142), Expect = 7.200e-9
Identity = 29/81 (35.80%), Postives = 44/81 (54.32%), Query Frame = -1
Query: 1372 LVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDATRYPDVASFYQIRGYPTIKYINGTDI-YTHDGDRTVEHLILF 1608
            LV FYAPWCG+C+  +P +      ++D P I    ID T+   + + Y +RGYPTI Y +       ++G RT +  I +
Sbjct:  417 LVMFYAPWCGHCKHTKPEFTAAATALQDDPRIAFVAIDCTKLAALCAKYNVRGYPTILYFSYLKTKLDYNGGRTSKDFIAY 497          

HSP 3 Score: 57.3806 bits (137), Expect = 2.719e-8
Identity = 34/101 (33.66%), Postives = 52/101 (51.49%), Query Frame = -1
Query: 1333 DKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKI-ARIDATRYPD--VASFYQIRGYPTIKYI-NGTDIYTHDGDRTVEHLILFSKRALGPAVKHLP 1623
            D  P LV FY PWCG+C+K++P Y +    +K     I A ++  R  +  +   + I G+PT+ Y  NG   +T++G+   E L+ F    L P  K  P
Sbjct:  161 DIRPMLVMFYVPWCGFCKKMKPEYGKASTELKTKGGYILAAMNVERQENAPIRKMFNITGFPTLIYFENGKLRFTYEGENNKEALVSF---MLNPNAKPTP 258          
BLAST of SMED30003142 vs. Ensembl Zebrafish
Match: tmx3a (thioredoxin related transmembrane protein 3a [Source:ZFIN;Acc:ZDB-GENE-050522-396])

HSP 1 Score: 136.346 bits (342), Expect = 3.142e-34
Identity = 112/422 (26.54%), Postives = 198/422 (46.92%), Query Frame = -1
Query:  400 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEA 1659
            V EL+  F E      WLV+FYAPWC YC   EP++ E+ A +K L  P+ + +ID T +  +A+ + IRGYPTIK   G   + + G RT + +I F+ R  GP V+ L S   F   +  H  +  F+YIG   S LK  Y   A    + +YF++   + +P++    L    +++  ++GT  ++                ++  L +WI+  RF ++ +I  + L+++  L K + + + +    S+ +       ER S+  +             +  ++D  F +      +N L M  L +P++++        YI P+S+     + I++  +  FL+S   G     GG GF    +R  Y     ++  V T P+ + F++G+P+ V+  + YA C    +D +    EA
Sbjct:   24 VEELDDKFTEFRQNELWLVEFYAPWCAYCHTFEPVWTEVGAELKSLGSPVNVGKIDTTAHTSIATEFNIRGYPTIKLFKGDLSFDYKGPRTKDGIIEFTNRVSGPVVRPLSSVQLFQHVMSRH--DVIFVYIGGE-SLLKKEYYKAATEFIVHTYFFTASEEILPKAV--TLQDVPAVAVFKDGTYYIYN-------------EFIDGDLSSWINRERFLSYFQIDSYSLYQMGELSKLVALAVVDEKNPSEESIRYKTLMERVSTEYR-------------DHYKSDFQFGYVDGNEYVNGLIMGELAMPSIIVLNMSIDGYYI-PESST----ETIED--LLQFLNSVLDGSTTLLGGNGFWQCAKRIFYKGKNTVMSMVETAPVFSCFVLGLPVGVVVLVIYATCTAVPADDEKPEEEA 407          
BLAST of SMED30003142 vs. Ensembl Zebrafish
Match: tmx3a (thioredoxin related transmembrane protein 3a [Source:ZFIN;Acc:ZDB-GENE-050522-396])

HSP 1 Score: 136.346 bits (342), Expect = 3.538e-34
Identity = 114/422 (27.01%), Postives = 199/422 (47.16%), Query Frame = -1
Query:  400 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEA 1659
            V EL+  F E      WLV+FYAPWC YC   EP++ E+ A +K L  P+ + +ID T +  +A+ + IRGYPTIK   G   + + G RT + +I F+ R  GP V+ L S   F   +  H  +  F+YIG   S LK  Y   A    + +YF++   + +P++         +L D     + V+K     +++   E   ++  L +WI+  RF ++ +I  + L+++  L K + + + +    S+ +       ER S+  +             +  ++D  F +      +N L M  L +P++++        YI P+S+     + I++  +  FL+S   G     GG GF    +R  Y     ++  V T P+ + F++G+P+ V+  + YA C    +D +    EA
Sbjct:   23 VEELDDKFTEFRQNELWLVEFYAPWCAYCHTFEPVWTEVGAELKSLGSPVNVGKIDTTAHTSIATEFNIRGYPTIKLFKGDLSFDYKGPRTKDGIIEFTNRVSGPVVRPLSSVQLFQHVMSRH--DVIFVYIGGE-SLLKKEYYKAATEFIVHTYFFTASEEILPKAV--------TLQDVP--AVAVFKDG---TYYIYNEF--IDGDLSSWINRERFLSYFQIDSYSLYQMGELSKLVALAVVDEKNPSEESIRYKTLMERVSTEYR-------------DHYKSDFQFGYVDGNEYVNGLIMGELAMPSIIVLNMSIDGYYI-PESST----ETIED--LLQFLNSVLDGSTTLLGGNGFWQCAKRIFYKGKNTVMSMVETAPVFSCFVLGLPVGVVVLVIYATCTAVPADDEKPEEEA 406          
BLAST of SMED30003142 vs. Ensembl Zebrafish
Match: tmx3a (thioredoxin related transmembrane protein 3a [Source:ZFIN;Acc:ZDB-GENE-050522-396])

HSP 1 Score: 110.153 bits (274), Expect = 1.141e-27
Identity = 54/136 (39.71%), Postives = 76/136 (55.88%), Query Frame = -1
Query: 1258 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRS 1659
            V EL+  F E      WLV+FYAPWC YC   EP++ E+ A +K L  P+ + +ID T +  +A+ + IRGYPTIK   G   + + G RT + +I F+ R  GP V+ L S   F   +  H  +  F+YIG  S
Sbjct:   23 VEELDDKFTEFRQNELWLVEFYAPWCAYCHTFEPVWTEVGAELKSLGSPVNVGKIDTTAHTSIATEFNIRGYPTIKLFKGDLSFDYKGPRTKDGIIEFTNRVSGPVVRPLSSVQLFQHVMSRH--DVIFVYIGGES 156          
BLAST of SMED30003142 vs. Ensembl Zebrafish
Match: tmx3b (thioredoxin related transmembrane protein 3b [Source:ZFIN;Acc:ZDB-GENE-060901-5])

HSP 1 Score: 81.2629 bits (199), Expect = 2.512e-18
Identity = 32/60 (53.33%), Postives = 45/60 (75.00%), Query Frame = -1
Query: 1438 WLVKFYAPWCGYCRKLEPIYNELEAHI--KDLPIKIARIDATRYPDVASFYQIRGYPTIK 1611
            WLV FYAPWCGYC+KLEP++ E+ A +     P+++ ++DAT Y  +AS + +RGYPTIK
Sbjct:   37 WLVDFYAPWCGYCKKLEPVWEEVGAELSRSGSPVRVGKMDATAYSGMASEFGVRGYPTIK 96          
BLAST of SMED30003142 vs. Ensembl Zebrafish
Match: pdia3 (protein disulfide isomerase family A, member 3 [Source:ZFIN;Acc:ZDB-GENE-031002-9])

HSP 1 Score: 83.1889 bits (204), Expect = 2.250e-16
Identity = 42/133 (31.58%), Postives = 70/133 (52.63%), Query Frame = -1
Query: 1213 LVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLR 1608
            LV+F+APWCG+C++L P Y      +K + + +A++D T    V   Y + GYPT+K + +G D   +DG RT + ++   K+  GPA   L +E  F +            YIGDR + +   + D  ++ +
Sbjct:    4 LVEFFAPWCGHCKRLAPEYEAAATRLKGI-VPLAKVDCTANSKVCGKYGVSGYPTLKIFRDGEDSGGYDGPRTADGIVSHLKKQAGPASVELKNEADFEK------------YIGDRDASVVGFFADGGSAAQ 123          

HSP 2 Score: 66.6254 bits (161), Expect = 3.921e-11
Identity = 36/86 (41.86%), Postives = 52/86 (60.47%), Query Frame = -1
Query: 1363 LVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDATRYPDVASFYQIRGYPTIKYINGT---DIYTHDGDRTVEHLILFSKR 1608
            L++FYAPWCG+C+ LEP Y EL   + + P I IA++DAT   DV S Y++ G+PTI +       +   ++G R V   I + KR
Sbjct:  353 LIEFYAPWCGHCKSLEPKYKELGEKLSEDPNIVIAKMDATAN-DVPSPYEVSGFPTIYFSPAGRKQNPKKYEGGREVSDFISYLKR 437          
BLAST of SMED30003142 vs. Ensembl Xenopus
Match: nup35 (nucleoporin 35kDa [Source:Xenbase;Acc:XB-GENE-980941])

HSP 1 Score: 172.94 bits (437), Expect = 8.653e-47
Identity = 117/417 (28.06%), Postives = 198/417 (47.48%), Query Frame = -1
Query:  421 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNE--LEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESST--MKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDA 1659
            V +L+ +F E      WLV FYAPWCG+C+KLEP++NE  +E      PI++ ++DAT +  +AS + +RG+PTIK + G   Y + G RT E ++ F+ R  GP ++ LPS+  F    + H     F+Y+G  S+ LK  Y ++A+ L + +YF+S     +P+    ++ LN      E   +LV+K      +   E     +  L +W++  RF  +  I    L+                   +      +   E+ +S    +L S A+ +A    +    D  F        +N L M  L +P  ++    N   ++       ++++  +E     F++S   G  E  GG G   +++R  YD  + ++    ++P+L  F+ G+PL VIS +CY IC  DT D 
Sbjct:   46 VEDLDDSFKENRKDDIWLVDFYAPWCGHCKKLEPVWNEVGIEMRSSGSPIRVGKMDATVHSSIASEFGVRGFPTIKVLKGDMAYNYRGPRTKEDIVEFANRVAGPLIRPLPSQQMFDHVQKRH--SVLFVYVGVEST-LKEKYIEVASELIVYTYFFSSSEDVLPK----YVTLN------EVPAVLVFKDSTYFVYDEYE-----DGDLSSWVNKERFEGYLHIDGFTLY---------------ELGDTGKLVAVAVIDEKNNSVEHTRLKSIAQDVAKNYRDHFHRDFQFGHMDGNDYINSLLMDELSIPTFVVLNTSNQQYFLPS-----KQIENTEEMI--QFINSILDGTAEAQGGDGILQRIKRVFYDAKSTVVSVFKSSPLLGCFLFGLPLGVISIMCYGICTADTEDG 422          
BLAST of SMED30003142 vs. Ensembl Xenopus
Match: nup35 (nucleoporin 35kDa [Source:Xenbase;Acc:XB-GENE-980941])

HSP 1 Score: 168.703 bits (426), Expect = 2.185e-45
Identity = 118/416 (28.37%), Postives = 198/416 (47.60%), Query Frame = -1
Query:  421 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNE--LEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDE-QNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDA 1659
            V +L+ +F E      WLV FYAPWCG+C+KLEP++NE  +E      PI++ ++DAT +  +AS + +RG+PTIK + G   Y + G RT E ++ F+ R  GP ++ LPS+  F    + H     F+Y+G  S+ LK  Y ++A+ L + +YF+S     +P+    ++ LN      E   +LV+K      +   E     +  L +W++  RF  +  I    L+EL      + I+I  +                         F+ K   +++ D    D  F        +N L M  L +P  ++    N   ++       ++++  +E     F++S   G  E  GG G   +++R  YD  + ++    ++P+L  F+ G+PL VIS +CY IC  DT D 
Sbjct:   28 VEDLDDSFKENRKDDIWLVDFYAPWCGHCKKLEPVWNEVGIEMRSSGSPIRVGKMDATVHSSIASEFGVRGFPTIKVLKGDMAYNYRGPRTKEDIVEFANRVAGPLIRPLPSQQMFDHVQKRH--SVLFVYVGVEST-LKEKYIEVASELIVYTYFFSSSEDVLPK----YVTLN------EVPAVLVFKDSTYFVYDEYE-----DGDLSSWVNKERFEGYLHIDGFTLYELGDTGSLVSIIISAACY-----------------------FSLKYVAKNYRDHFHRDFQFGHMDGNDYINSLLMDELSIPTFVVLNTSNQQYFLPS-----KQIENTEEMI--QFINSILDGTAEAQGGDGILQRIKRVFYDAKSTVVSVFKSSPLLGCFLFGLPLGVISIMCYGICTADTEDG 395          
BLAST of SMED30003142 vs. Ensembl Xenopus
Match: nup35 (nucleoporin 35kDa [Source:Xenbase;Acc:XB-GENE-980941])

HSP 1 Score: 162.54 bits (410), Expect = 2.235e-43
Identity = 112/403 (27.79%), Postives = 193/403 (47.89%), Query Frame = -1
Query:  421 WLVKFYAPWCGYCRKLEPIYNE--LEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLF----SFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDA 1611
            WL  FYAPWCG+C+KLEP++NE  +E      PI++ ++DAT +  +AS + +RG+PTIK + G   Y + G RT E ++ F+ R  GP ++ LPS+  F    + H     F+Y+G  S+ LK  Y ++A+ L + +YF+S     +P+    ++ LN      E   +LV+K      +   E     +  L +W++  RF  +  I    L+          +      +   +    +++ E  S ++ L     SF     F+  + + ND           +N L M  L +P  ++    N   ++       ++++  +E     F++S   G  E  GG G   +++R  YD  + ++    ++P+L  F+ G+PL VIS +CY IC  DT D 
Sbjct:   26 WL--FYAPWCGHCKKLEPVWNEVGIEMRSSGSPIRVGKMDATVHSSIASEFGVRGFPTIKVLKGDMAYNYRGPRTKEDIVEFANRVAGPLIRPLPSQQMFDHVQKRH--SVLFVYVGVEST-LKEKYIEVASELIVYTYFFSSSEDVLPK----YVTLN------EVPAVLVFKDSTYFVYDEYE-----DGDLSSWVNKERFEGYLHIDGFTLY---------ELGDTGKLVAVAVIDEKNNSVEHTSVSVTLLYRFCSFLVGRDFQFGHMDGNDY----------INSLLMDELSIPTFVVLNTSNQQYFLPS-----KQIENTEEMI--QFINSILDGTAEAQGGDGILQRIKRVFYDAKSTVVSVFKSSPLLGCFLFGLPLGVISIMCYGICTADTEDG 382          
BLAST of SMED30003142 vs. Ensembl Xenopus
Match: nup35 (nucleoporin 35kDa [Source:Xenbase;Acc:XB-GENE-980941])

HSP 1 Score: 162.155 bits (409), Expect = 2.542e-43
Identity = 110/400 (27.50%), Postives = 192/400 (48.00%), Query Frame = -1
Query:  421 KFYAPWCGYCRKLEPIYNE--LEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLF----SFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDA 1602
            +FYAPWCG+C+KLEP++NE  +E      PI++ ++DAT +  +AS + +RG+PTIK + G   Y + G RT E ++ F+ R  GP ++ LPS+  F    + H     F+Y+G  S+ LK  Y ++A+ L + +YF+S     +P+    ++ LN      E   +LV+K      +   E     +  L +W++  RF  +  I    L+          +      +   +    +++ E  S ++ L     SF     F+  + + ND           +N L M  L +P  ++    N   ++       ++++  +E     F++S   G  E  GG G   +++R  YD  + ++    ++P+L  F+ G+PL VIS +CY IC  DT D 
Sbjct:   20 QFYAPWCGHCKKLEPVWNEVGIEMRSSGSPIRVGKMDATVHSSIASEFGVRGFPTIKVLKGDMAYNYRGPRTKEDIVEFANRVAGPLIRPLPSQQMFDHVQKRH--SVLFVYVGVEST-LKEKYIEVASELIVYTYFFSSSEDVLPK----YVTLN------EVPAVLVFKDSTYFVYDEYE-----DGDLSSWVNKERFEGYLHIDGFTLY---------ELGDTGKLVAVAVIDEKNNSVEHTSVSVTLLYRFCSFLVGRDFQFGHMDGNDY----------INSLLMDELSIPTFVVLNTSNQQYFLPS-----KQIENTEEMI--QFINSILDGTAEAQGGDGILQRIKRVFYDAKSTVVSVFKSSPLLGCFLFGLPLGVISIMCYGICTADTEDG 375          
BLAST of SMED30003142 vs. Ensembl Xenopus
Match: nup35 (nucleoporin 35kDa [Source:Xenbase;Acc:XB-GENE-980941])

HSP 1 Score: 146.747 bits (369), Expect = 1.202e-37
Identity = 114/419 (27.21%), Postives = 189/419 (45.11%), Query Frame = -1
Query:  421 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNE--LEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLF----SFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDA 1659
            V +L+ +F E      WLV FYAPWCG+C+KLEP++NE  +E      PI++ ++DAT +  +AS + +RG+PTIK + G   Y + G RT E ++ F+ R  GP ++ LPS+  F    + H     F+Y+G  S+ LK  Y ++A+ L + +YF+S     +P+    ++ LN      E   +LV+K      +   E     +  L +W++  RF  +  I    L+          +      +   +    +++ E  S ++ L     SF     F+  + + ND           +N L M  L +P  ++    N   Y  P        + I  +F++  L    + E+       F  K                 ++P+L  F+ G+PL VIS +CY IC  DT D 
Sbjct:   28 VEDLDDSFKENRKDDIWLVDFYAPWCGHCKKLEPVWNEVGIEMRSSGSPIRVGKMDATVHSSIASEFGVRGFPTIKVLKGDMAYNYRGPRTKEDIVEFANRVAGPLIRPLPSQQMFDHVQKRH--SVLFVYVGVEST-LKEKYIEVASELIVYTYFFSSSEDVLPK----YVTLN------EVPAVLVFKDSTYFVYDEYE-----DGDLSSWVNKERFEGYLHIDGFTLY---------ELGDTGKLVAVAVIDEKNNSVEHTSVSVTLLYRFCSFLVGRDFQFGHMDGNDY----------INSLLMDELSIPTFVVLNTSNQQ-YFLPSKQIENTEEMI--QFINSILDG--TAEVSCLPTCFFCLKQS------------VFKSSPLLGCFLFGLPLGVISIMCYGICTADTEDG 392          
BLAST of SMED30003142 vs. Ensembl Mouse
Match: Tmx3 (thioredoxin-related transmembrane protein 3 [Source:MGI Symbol;Acc:MGI:2442418])

HSP 1 Score: 170.244 bits (430), Expect = 5.233e-46
Identity = 123/425 (28.94%), Postives = 200/425 (47.06%), Query Frame = -1
Query:  397 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESST--MKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEAR 1659
            V +LN +F +      WLV FYAPWCG+C+KLEPI+NE+   +K +  P+K+ ++DAT Y  +AS + +RGYPTIK + G   Y + G RT + +I F+ R  G  ++ LPS+  F    + H    FF+YIG  S  LK  Y D A+ L + +YF+S     +P+  Y  L         E   +LV+K D    +   E     +  L +WIS  RF  +  +    L+                   +      +   E+ +S    +L S  +++A +  +    D  F        +N L M  L VP +++    N   ++    + + K    D   +  F++S   G +   GG     +++R  +D  + ++    ++P++  F+ G+PL VIS +CY I    T+D D   IE R
Sbjct:   31 VEDLNESFKDNRKDDIWLVDFYAPWCGHCKKLEPIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIKLLKGDLAYNYRGPRTKDDIIEFAHRVSGALIRPLPSQQMFDHVRKRH--RVFFVYIGGESP-LKEKYIDAASELIVYTYFFSASEDVVPE--YVTL--------KEMPAVLVFKDDTYFVYDEYE-----DGDLSSWISRERFQNYLTMDGFLLY---------------ELGDTGKLVAIAVIDEKNTSLEHTRLKSIIQEVARDFRDHFHRDFQFGHMDGNDYINTLLMDELTVPTIVVLNTSNQQYFLL---DRHIK----DASDMVQFINSILDGTVPAQGGDSIFQRLKRIVFDAKSTIVSIFKSSPLMGCFLFGLPLGVISIMCYGIY---TADTDGGYIEER 412          
BLAST of SMED30003142 vs. Ensembl Mouse
Match: Tmx3 (thioredoxin-related transmembrane protein 3 [Source:MGI Symbol;Acc:MGI:2442418])

HSP 1 Score: 137.887 bits (346), Expect = 3.629e-35
Identity = 82/222 (36.94%), Postives = 120/222 (54.05%), Query Frame = -1
Query: 1000 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTF 1659
            V +LN +F +      WLV FYAPWCG+C+KLEPI+NE+   +K +  P+K+ ++DAT Y  +AS + +RGYPTIK + G   Y + G RT + +I F+ R  G  ++ LPS+  F    + H    FF+YIG  S  LK  Y D A+ L + +YF+S     +P+  Y  L         E   +LV+K D    +   E     +  L +WIS  RF  +
Sbjct:   31 VEDLNESFKDNRKDDIWLVDFYAPWCGHCKKLEPIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIKLLKGDLAYNYRGPRTKDDIIEFAHRVSGALIRPLPSQQMFDHVRKRH--RVFFVYIGGESP-LKEKYIDAASELIVYTYFFSASEDVVPE--YVTL--------KEMPAVLVFKDDTYFVYDEYE-----DGDLSSWISRERFQNY 234          
BLAST of SMED30003142 vs. Ensembl Mouse
Match: Pdia4 (protein disulfide isomerase associated 4 [Source:MGI Symbol;Acc:MGI:104864])

HSP 1 Score: 80.4925 bits (197), Expect = 2.429e-15
Identity = 36/86 (41.86%), Postives = 55/86 (63.95%), Query Frame = -1
Query: 1378 IADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIK--DLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLI 1629
            +ADK   L++FYAPWCG+C++  P Y ++ + +K  D PI +A+IDAT    +AS + + GYPTIK +       +DG RT E ++
Sbjct:   72 VADKDTVLLEFYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASMLASKFDVSGYPTIKILKKGQAVDYDGSRTQEEIV 157          

HSP 2 Score: 75.8702 bits (185), Expect = 7.945e-14
Identity = 44/136 (32.35%), Postives = 73/136 (53.68%), Query Frame = -1
Query: 1213 LVKFYAPWCGYCRKLEPIYNELEAHI--KDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAI-ESHLMETFFLYIGDRS-SYLKAVYDDLANSLR 1608
            LV+FYAPWCG+C+KL P Y +    +  +  PI +A++DAT   D+A  + + GYPT+K       + ++G R    ++ +     GP  K + +  +  E + +   +    L+ GD   +YL+  Y D AN+LR
Sbjct:  194 LVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATEQTDLAKRFDVSGYPTLKIFRKGRPFDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFLKDGDDVVIIGLFQGDGDPAYLQ--YQDAANNLR 327          

HSP 3 Score: 64.3142 bits (155), Expect = 3.200e-10
Identity = 34/84 (40.48%), Postives = 48/84 (57.14%), Query Frame = -1
Query: 1372 LVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDATRYPDVASFYQIRGYPTIKYINGTD----IYTHDGDRTVEHLILF 1608
            L++FYAPWCG+C++LEPIY  L    K    + IA++DAT        Y++ G+PTI +    D    I    G+R +EHL  F
Sbjct:  543 LIEFYAPWCGHCKQLEPIYTSLGKKYKGQKDLVIAKMDATANDITNDQYKVEGFPTIYFAPSGDKKNPIKFEGGNRDLEHLSKF 626          
BLAST of SMED30003142 vs. Ensembl Mouse
Match: Pdia3 (protein disulfide isomerase associated 3 [Source:MGI Symbol;Acc:MGI:95834])

HSP 1 Score: 78.5666 bits (192), Expect = 9.141e-15
Identity = 47/151 (31.13%), Postives = 79/151 (52.32%), Query Frame = -1
Query: 1240 NVIELN-----SNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAI--ESHLMETFF--LYIGDRSSYLKAV 1662
            +V+EL      S   +    G  LV+F+APWCG+C++L P Y      +K + + +A++D T   +  + Y + GYPT+K + +G +   +DG RT + ++   K+  GPA   L +E  F + I  +   +  FF  L+    S +LKA 
Sbjct:   26 DVLELTDENFESRVSDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGI-VPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASVVGFFRDLFSDGHSEFLKAA 175          

HSP 2 Score: 67.3958 bits (163), Expect = 2.959e-11
Identity = 37/86 (43.02%), Postives = 53/86 (61.63%), Query Frame = -1
Query: 1363 LVKFYAPWCGYCRKLEPIYNEL-EAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYT---HDGDRTVEHLILFSKR 1608
            L++FYAPWCG+C+ LEP Y EL E   KD  I IA++DAT   DV S Y+++G+PTI +       T   ++G R +   I + +R
Sbjct:  398 LIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATAN-DVPSPYEVKGFPTIYFSPANKKLTPKKYEGGRELNDFISYLQR 482          
BLAST of SMED30003142 vs. Ensembl Mouse
Match: Pdia6 (protein disulfide isomerase associated 6 [Source:MGI Symbol;Acc:MGI:1919103])

HSP 1 Score: 74.7146 bits (182), Expect = 1.114e-13
Identity = 41/116 (35.34%), Postives = 63/116 (54.31%), Query Frame = -1
Query: 1291 WLVKFYAPWCGYCRKLEPIYNELEAHIKDL---PIKIARIDATRYPDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLI-----LFSKRALGPAVKHLPSEGRFHEAIESHLM 1611
            W+V+FYAPWCG+C+ LEP +      +K+     +K+A +DAT    +AS Y I+G+PTIK +  G     +DG RT   ++     LFS  A  P +  + +E    +  E H +
Sbjct:  186 WMVEFYAPWCGHCKNLEPEWAAAATEVKEQTKGKVKLAAVDATMNQVLASRYGIKGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKKTCEEHQL 301          

HSP 2 Score: 73.1738 bits (178), Expect = 3.339e-13
Identity = 41/112 (36.61%), Postives = 63/112 (56.25%), Query Frame = -1
Query: 1342 NVIELN-SNFIE--IADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHD--GDRTVEHLILFSKRALGPAVK 1662
            +VIEL  SNF    I   G WLV+FYAPWCG+C++L P + +    +KD+ +K+  ++A ++  +   Y ++G+PTIK          D  G RT E ++  +  AL   VK
Sbjct:   31 DVIELTPSNFNREVIQSDGLWLVEFYAPWCGHCQRLTPEWKKAATALKDV-VKVGAVNADKHQSLGGQYGVQGFPTIKIFGANKNKPEDYQGGRTGEAIVDAALSALRQLVK 141          
BLAST of SMED30003142 vs. UniProt/SwissProt
Match: sp|Q6GNG3|TMX3_XENLA (Protein disulfide-isomerase TMX3 OS=Xenopus laevis OX=8355 GN=tmx3 PE=2 SV=1)

HSP 1 Score: 177.948 bits (450), Expect = 4.949e-48
Identity = 120/417 (28.78%), Postives = 198/417 (47.48%), Query Frame = -1
Query:  421 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIK--DLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESST--MKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDA 1659
            V +L+ +F E      WLV FYAPWCG+C+KLEP++NE+   I+    PI++ +IDAT Y  +AS + +RG+PTIK + G   Y + G RT E ++ F+ R  GP ++ LPS+  F    + H     F+Y+G  S+ LK  + ++A+ L + +YF+S     +P+    ++ LN      E   +LV+K      +   E     +  L +W++  RF  +  I    L+                   +      +   E+ +S    ++ S A+ +A  + N+   D  F        +N L M  L +P  ++    N   Y  P  +     + I       F++S   G  E  GG G   +++R  YD  + ++    ++P+L  F+ G+PL VIS +CY IC  DT D 
Sbjct:   28 VEDLDDSFKENRKDDIWLVDFYAPWCGHCKKLEPVWNEVGIEIRTSGSPIRVGKIDATVYSSIASEFGVRGFPTIKALKGDMAYNYRGPRTKEDIVEFANRVAGPLIRPLPSQQMFDHVKKRH--PVLFVYVGVEST-LKEKFIEVASELIVYTYFFSASEDVLPK----YVTLN------EVPAVLVFKDSTYFVYDEYE-----DGDLSSWVNKERFEGYLHIDGFTLY---------------ELGDTGKLVAVAVIDEKNNSIEHTRIKSIAQDVAKNNRNNFHRDFQFGHMDGNDYINSLLMDELSIPTFVVLNTSNQQ-YFLPSKHIENPEEMI------QFINSILDGTAEAQGGDGILQRIKRVFYDAKSTVVSVFKSSPLLGCFLFGLPLGVISIMCYGICTADTEDG 404          
BLAST of SMED30003142 vs. UniProt/SwissProt
Match: sp|Q5R875|TMX3_PONAB (Protein disulfide-isomerase TMX3 OS=Pongo abelii OX=9601 GN=TMX3 PE=2 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 5.832e-47
Identity = 122/425 (28.71%), Postives = 206/425 (48.47%), Query Frame = -1
Query:  397 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESST--MKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEAR 1659
            V +L+ +F E  +   WLV FYAPWCG+C+KLEPI+NE+   +K +  P+K+ ++DAT Y  +AS + +RGYPTIK + G   Y H G RT + +I F+ R  G  ++ LPS+  F    + H    FF+YIG  S  LK  Y D A+ L + +YF+S   + +P+  Y  L         E   +LV+K +    +   E     +  L +WI+  RF  +  +    L+                   +      +   E+ +S    +L S  +++A +  +    D  F        +N L M  L VP V++    N   ++       +++K +++  +  F+++   G +E  GG     +++R  +D  + ++    ++P++  F+ G+PL VIS +CY I    T+D D   IE R
Sbjct:   28 VEDLDESFKENRNDDIWLVDFYAPWCGHCKKLEPIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIKLLKGDLAYNHRGPRTKDDIIEFAHRVSGALIRPLPSQQMFEHMQKRH--RVFFVYIGGESP-LKEKYIDAASELIVYTYFFSASEEVVPE--YVTL--------KEMPAVLVFKDETYFVYDEYE-----DGDLSSWINRERFQNYLAMDGFLLY---------------ELGDTGKLVALAVIDEKNTSVEHTRLKSIIQEVARDYRDLFHRDFQFGHMDGNDYINTLLMDELTVPTVVVLNTSNQQYFLLD-----RQIKNVED--MVQFINNILDGTVEAQGGDSILQRLKRIVFDAKSTIVSIFKSSPLMGCFLFGLPLGVISIMCYGIY---TADTDGGYIEER 409          
BLAST of SMED30003142 vs. UniProt/SwissProt
Match: sp|Q96JJ7|TMX3_HUMAN (Protein disulfide-isomerase TMX3 OS=Homo sapiens OX=9606 GN=TMX3 PE=1 SV=2)

HSP 1 Score: 172.555 bits (436), Expect = 4.474e-46
Identity = 120/425 (28.24%), Postives = 206/425 (48.47%), Query Frame = -1
Query:  397 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESST--MKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEAR 1659
            V +L+ +F E  +   WLV FYAPWCG+C+KLEPI+NE+   +K +  P+K+ ++DAT Y  +AS + +RGYPTIK + G   Y + G RT + +I F+ R  G  ++ LPS+  F    + H    FF+Y+G  S  LK  Y D A+ L + +YF+S   + +P+  Y  L         E   +LV+K +    +   E     +  L +WI+  RF  +  +    L+                   +      +   E+ +S    +L S  +++A +  +    D  F        +N L M  L VP V++    N   ++       +++K +++  +  F+++   G +E  GG     +++R  +D  + ++    ++P++  F+ G+PL VIS +CY I    T+D D   IE R
Sbjct:   28 VEDLDESFKENRNDDIWLVDFYAPWCGHCKKLEPIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIKLLKGDLAYNYRGPRTKDDIIEFAHRVSGALIRPLPSQQMFEHMQKRH--RVFFVYVGGESP-LKEKYIDAASELIVYTYFFSASEEVVPE--YVTL--------KEMPAVLVFKDETYFVYDEYE-----DGDLSSWINRERFQNYLAMDGFLLY---------------ELGDTGKLVALAVIDEKNTSVEHTRLKSIIQEVARDYRDLFHRDFQFGHMDGNDYINTLLMDELTVPTVVVLNTSNQQYFLLD-----RQIKNVED--MVQFINNILDGTVEAQGGDSILQRLKRIVFDAKSTIVSIFKSSPLMGCFLFGLPLGVISIMCYGIY---TADTDGGYIEER 409          
BLAST of SMED30003142 vs. UniProt/SwissProt
Match: sp|Q8BXZ1|TMX3_MOUSE (Protein disulfide-isomerase TMX3 OS=Mus musculus OX=10090 GN=Tmx3 PE=1 SV=2)

HSP 1 Score: 170.244 bits (430), Expect = 3.660e-45
Identity = 123/425 (28.94%), Postives = 200/425 (47.06%), Query Frame = -1
Query:  397 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESST--MKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEAR 1659
            V +LN +F +      WLV FYAPWCG+C+KLEPI+NE+   +K +  P+K+ ++DAT Y  +AS + +RGYPTIK + G   Y + G RT + +I F+ R  G  ++ LPS+  F    + H    FF+YIG  S  LK  Y D A+ L + +YF+S     +P+  Y  L         E   +LV+K D    +   E     +  L +WIS  RF  +  +    L+                   +      +   E+ +S    +L S  +++A +  +    D  F        +N L M  L VP +++    N   ++    + + K    D   +  F++S   G +   GG     +++R  +D  + ++    ++P++  F+ G+PL VIS +CY I    T+D D   IE R
Sbjct:   31 VEDLNESFKDNRKDDIWLVDFYAPWCGHCKKLEPIWNEVGLEMKSIGSPVKVGKMDATSYSSIASEFGVRGYPTIKLLKGDLAYNYRGPRTKDDIIEFAHRVSGALIRPLPSQQMFDHVRKRH--RVFFVYIGGESP-LKEKYIDAASELIVYTYFFSASEDVVPE--YVTL--------KEMPAVLVFKDDTYFVYDEYE-----DGDLSSWISRERFQNYLTMDGFLLY---------------ELGDTGKLVAIAVIDEKNTSLEHTRLKSIIQEVARDFRDHFHRDFQFGHMDGNDYINTLLMDELTVPTIVVLNTSNQQYFLL---DRHIK----DASDMVQFINSILDGTVPAQGGDSIFQRLKRIVFDAKSTIVSIFKSSPLMGCFLFGLPLGVISIMCYGIY---TADTDGGYIEER 412          
BLAST of SMED30003142 vs. UniProt/SwissProt
Match: sp|O13704|YEU5_SCHPO (Thioredoxin domain-containing protein C13F5.05, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC13F5.05 PE=4 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 4.934e-18
Identity = 77/234 (32.91%), Postives = 116/234 (49.57%), Query Frame = -1
Query: 1000 NVIELNS-NFIE-IADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKD-LPIKIARIDATRYPDVASFYQIRGYPTIKYI------NGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLN-SSLSDTENGTILVWK-PDRKLSFHFKEEIS--SLNHSLQNWISFNRFSTF 1662
            N IELNS NF + +  KGP LV FYAPWCGYC+KL P Y +L +++   LP+     DA +   V S YQ++G+PTIK +      +      ++GDR+ + L  F   ++   VK L SE +  + I+     +  + I  +      +Y  L+N       F S+   F+P     +   N S+  DT +  IL  K P+   SF F   ++  S+   LQ+ I  N FS +
Sbjct:   32 NTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSIPSKVKILTSEAKTQKFIQDAQNSSKVILISQKMK-PTLLYKSLSNE------FSSLPFSFMPAKANVNDLFNISAYVDTSDLPILFIKHPNNGTSF-FSNSLNRDSIVEFLQSSIKDNNFSEY 257          
BLAST of SMED30003142 vs. TrEMBL
Match: A0A210QUZ2 (Protein disulfide-isomerase TMX3 OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT13753 PE=4 SV=1)

HSP 1 Score: 240.35 bits (612), Expect = 1.067e-68
Identity = 127/415 (30.60%), Postives = 230/415 (55.42%), Query Frame = -1
Query:  424 NVIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIG--DRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSD 1662
             V+EL+  F+++  + PWLV+FYAPWCG+C+KLEP ++++   +++ P+K+ ++D TR+  VAS + ++G+PTIK+ NG +IYTH GDRT E ++ F  RA GPAV++L S+G+F EA   H    FFLY+G  D    + + Y ++A  + ++SYFY+  +  +PQ           +      T+L +K    + +  ++ I +++ SLQ+WI+  RF  FP ++  ++             I  S  +  + +   +N E ++ + ++    K LA +  +   +   F+W +D+ ++N +AM  +  P V++  P  H  Y+         M+ + +     +L   +  ++   GG GF  +++R  YD I  ++    ++  L + + G+P +VIS +CY++CCMDT D
Sbjct:   24 QVLELDERFLDVYKEVPWLVEFYAPWCGHCKKLEPTFHQVSMSLRNTPVKVGKLDCTRFSSVASEFSVKGFPTIKFFNGENIYTHRGDRTKEDIMEFVNRAKGPAVRNLASKGKFEEAKNQHKDSAFFLYLGRQDPVDDMFSKYSNVAEQMLIQSYFYAGNSAVLPQ----------RIKPAVTPTVLAFKDQEVVEYKAQDGIVTMS-SLQDWINSERFPAFPLVTGANI-----------NDIAESGKILVMMAFDDENTESKAGSERIKDMVKVLATQHKDRFHSGFQFLWMSDMETVNSIAMAFMKGPVVMLLNPTTHYYYMPLTPIQDMAMEQLMQ-----YLEDVRDEKVPALGGTGFFQRLKRVFYDLIVMVVGVWQSSRWLFLLMFGLPTTVISVVCYSLCCMDTVD 411          
BLAST of SMED30003142 vs. TrEMBL
Match: A0A267EQ68 (Thioredoxin domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009031g3 PE=4 SV=1)

HSP 1 Score: 226.483 bits (576), Expect = 3.126e-63
Identity = 131/418 (31.34%), Postives = 211/418 (50.48%), Query Frame = -1
Query:  424 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENG------TILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSD 1659
            V+E+   FI       +LVKFYAPWCG+CR +EP Y E+   +   P+ +ARIDAT +   A+ +++RG+PT+ Y+ G   +   G+RT + ++ F  R  G  VK + S G+F+E   + + + FFL+IG   S   AV++ +A  +R  S FY  + Q +    +   DL +   D  +G       +L +K +  + F  + +    N SL  W++  RF  +PK+     +               S   S          E +    +     + LAF S + E  ++ F W+    + +D+AMT+L  PNV++  P +   Y+ PD +    ++      +   +    +GE+  YGG G    ++R  YDF TGL  FV + P LA+ +IG P++V+S LCYAICCMDT+D
Sbjct:   38 VVEVGEEFIGRDASQAFLVKFYAPWCGHCRNMEPAYEEVSDRLAGSPVTVARIDATVHQAAAAHFRVRGFPTLIYVKGDTRHEFRGERTADEIVKFVNRVSGDPVKVIASPGKFNEVFATSVKDPFFLFIGSEESKEFAVFNGIAGQMRTDSSFYRADAQLLANLGF---DLAAKRLDRGHGGDIAEPCVLAFKDNGFVLFRGQFD----NDSLGVWMTNERFHIYPKLGDFLFY---------------SFYPSTKLLVAIVTNESDPEERRFVQLGRALAF-SMDLELANVQFGWAGSAKAASDMAMTSLPTPNVMLLNPNSTRYYLRPDYHLPISVRDETVGSLKRLVLQGLNGELPAYGGGGLIEGLKRTVYDFATGLAGFVLSHPGLALVVIGAPVAVVSILCYAICCMDTTD 432          
BLAST of SMED30003142 vs. TrEMBL
Match: A0A2T7NH06 (Thioredoxin domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_18619 PE=4 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 1.274e-62
Identity = 131/446 (29.37%), Postives = 237/446 (53.14%), Query Frame = -1
Query:  340 NFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSY--LKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQ--PWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCM---DTSDADNRRIEARQQKK-----IRIPLYEVALKSSEE 1641
            +F+E      WLV+FYAPWCG+C+KLEP+Y E+   +++ P+K+ ++D TR+ D+A+ + IRG+PTIK++ G  ++TH GDR+ E ++ F+ RA GPAV+ L S G+ +EA   H  E FFLYIGD      L   Y  +A+ + ++ Y+Y+ +   +             +S   + T+L +K   +  F   E ++++  +L++WI+  R+  FP+I     W         I L+ ++     I     +  ++     +L + A+ +A +  +   ++  F+W TD+ ++N + M+ L  P +L+ +  P  + +   P SN       I       FL   ++G ++ +GG GF  +++R  YD +T +I     +  L + + G+P  +IS +CY++CCM   D S  ++   E RQ ++      + P Y+  LK+ ++
Sbjct:    4 SFLEKKGDDMWLVEFYAPWCGHCKKLEPVYREVAHELRNSPVKVGKVDCTRFTDLAAHFSIRGFPTIKFVKGNQVFTHRGDRSKEGILEFASRAQGPAVRKLSSVGKLNEARSKHSDEVFFLYIGDDDEQDDLYQKYQTVADKMMVQGYYYAGKKHIL-----------EDISTKNHPTVLAFKDRMQYEFLPPEGVATM-EALEHWINTERYPAFPQI-----W------GGAINLLADTKKYLVIIVVDPEIKDKMDGNNRLKNIAEAIALDQRDKYHSEFQFLWMTDVETVNTITMSFLTPPVLLVLEVDPQYYYLPDLPASN-------ITINTFMQFLDGIRNGTVQAHGGNGFFMRVKRLLYDIMTTIISIWQASRWLFLLVFGLPTLIISIICYSLCCMEPLDDSYLEDEEDELRQLQQESAGDAKAPDYDEELKNEQD 419          
BLAST of SMED30003142 vs. TrEMBL
Match: V4B8B0 (Thioredoxin domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_206090 PE=4 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.770e-62
Identity = 126/405 (31.11%), Postives = 211/405 (52.10%), Query Frame = -1
Query:  424 GPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLP--IKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYI--GDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQ---NDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSD 1617
            G WL++FYAPWCG+CRKLEP+Y ++   +K+    + +++IDATRY +VAS + +RG+PT+K++NG  +YTH GDR+ E ++ F  +A GPAV+++ S G+F+E    H    FF+++  GD +  L   Y  LA    + SYFYS     +P            ++     T+LV+K      F   E        L+ W++  RF  FPK+    L               N  + +          +  S   +     K ++  + N  Q    D  F+W  D  + N + M+ L +P++++ +  +   YI  D N    +  + E+ + DFL   + G+++ YGG GF +K++RAG+DF+T ++     +  L + + G+P  +IS +CY++CCM+T D
Sbjct:    5 GMWLIEFYAPWCGHCRKLEPVYRQVFTELKNSGSGVIVSKIDATRYSNVASEFDVRGFPTLKFVNGDKVYTHRGDRSKEDILDFVNKAKGPAVRNIASVGKFNEIKTLHGKSVFFMFVGNGDENDDLFKKYSSLAEKHIIESYFYSGPKNSLPD----------KVTIKTFPTVLVFKDKNIFEFDVGESEVVTKDKLEKWLNRERFPAFPKVQGGGL---------------NELVTAAKYLVIVVVNKDSSVKNECKKVKKVVSNVALNHRQKYHQDFQFLWMPDEETANSITMSFLNIPSIVVLETASQLHYI-SDKN----VTDLTEQSIQDFLDGIKVGKVDAYGGTGFLSKLKRAGFDFLTTVLSIWQASRWLFLLMFGLPTVIISVVCYSLCCMETID 379          
BLAST of SMED30003142 vs. TrEMBL
Match: A0A0X3PAE8 (Protein disulfide-isomerase TMX3 OS=Schistocephalus solidus OX=70667 GN=TMX3 PE=4 SV=1)

HSP 1 Score: 223.787 bits (569), Expect = 6.489e-62
Identity = 130/427 (30.44%), Postives = 220/427 (51.52%), Query Frame = -1
Query:  385 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHI-KDLP-IKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEARQQKK 1659
            VIEL S  I     G WL+KFYAPWC +CR+LEP+Y+E+   + + LP + +ARID    P V++ + IRGYPTI +++    Y + G+R  + L+ F  RA GPAVK L S+    + ++ H  E  F++ G   S L   Y+ +A+ LRL+  F ++E    P+                + ++ V+K     SF  + +   L   ++ W+   R   F +IS   +      +    I + +S+    +        E + ++ +L +    LA +  +       FVW +D+ S++ L MT+L  PN+ ++ P      +HP  N+ ++++ +    V +FL +  SGE+  YGG G  A+++RA Y+F +G+++ V  +P+LA+ + G PL  +S LCY IC     D D   +E R + +
Sbjct:   25 VIELTSESISKVHTGLWLLKFYAPWCSFCRQLEPVYHEVALTLSRILPEVHVARIDVPANPAVSADFGIRGYPTIIFLHNGHRYPYQGERAADDLVKFVIRASGPAVKKLGSQEELGKELQVHKDEALFVFSGSTKSALWEAYNKVADELRLQVSFVALEKDVSPEPV--------------SASLRVFKDGIHFSFKPQADADDLREEVRRWVLLERHPAFQRISALGI-----RRLVAEINLVSSAQSLSLVVFVLSPDETDVASHRLKTIGSSLASDRSDSLLQHFRFVWCSDVDSVSRLVMTSLQPPNLFVYGP-EDIFALHPLYNSIKELRELTAVEVRNFLKAVASGEVRTYGGSGIFARLKRASYEFTSGVLDLVYNSPVLALLVFGFPLGCLSCLCYCICWGSFED-DPEAVEFRARSR 430          
BLAST of SMED30003142 vs. Ensembl Cavefish
Match: tmx3a (protein disulfide-isomerase TMX3-like [Source:NCBI gene;Acc:103028107])

HSP 1 Score: 136.732 bits (343), Expect = 2.178e-34
Identity = 103/369 (27.91%), Postives = 171/369 (46.34%), Query Frame = -1
Query:  565 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTI--YIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKM 1659
            V EL+  F +     PWLV+FYAPWC +C+  EPI+ E+ A +K L  P+ + +ID T Y +VA+ Y IRGYP I  + G   Y + G RT + ++ F+ R  GP V+ L S   F  A   H  + FFLYIG RS  LK +Y  +A    + +YF+S     +P+S    L    +++  ++GT  V+  +              + +L  W+   RF  + ++    L+                     +     +     + +++  S  +K+A E  +  + D  F +      +N   M  L +P++++    N TI  Y  P+S    K++  +E  +  FL S   G+ +  GG GF  ++
Sbjct:   23 VEELDDKFNDFRKSEPWLVEFYAPWCEFCKTFEPIWYEVGAELKSLGSPVTVGKIDTTVYTNVATEYAIRGYPAIILLKGEKSYEYKGPRTKDGILEFTNRVAGPVVRSLTSAQLFQHATSRH--DLFFLYIGGRSP-LKGLYYKVAADFIIHNYFFSAPEDVLPKSV--TLQEVPAVAVFKDGTYYVYNEEH-------------DGNLSAWVDQERFRCYFQMDSFTLY-------------QMGDRTKLVALTVLEEKNPSADSIRYKSLMEKVATEYRDHYKTDFQFGYMHGNDYINGFIMGELEIPSLIVM---NMTIDGYYLPES----KIETTEE--LLQFLSSVLDGQSQLLGGNGFLRRI 351          
BLAST of SMED30003142 vs. Ensembl Cavefish
Match: tmx3a (protein disulfide-isomerase TMX3-like [Source:NCBI gene;Acc:103028107])

HSP 1 Score: 136.732 bits (343), Expect = 2.178e-34
Identity = 103/369 (27.91%), Postives = 171/369 (46.34%), Query Frame = -1
Query:  565 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTI--YIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKM 1659
            V EL+  F +     PWLV+FYAPWC +C+  EPI+ E+ A +K L  P+ + +ID T Y +VA+ Y IRGYP I  + G   Y + G RT + ++ F+ R  GP V+ L S   F  A   H  + FFLYIG RS  LK +Y  +A    + +YF+S     +P+S    L    +++  ++GT  V+  +              + +L  W+   RF  + ++    L+                     +     +     + +++  S  +K+A E  +  + D  F +      +N   M  L +P++++    N TI  Y  P+S    K++  +E  +  FL S   G+ +  GG GF  ++
Sbjct:   23 VEELDDKFNDFRKSEPWLVEFYAPWCEFCKTFEPIWYEVGAELKSLGSPVTVGKIDTTVYTNVATEYAIRGYPAIILLKGEKSYEYKGPRTKDGILEFTNRVAGPVVRSLTSAQLFQHATSRH--DLFFLYIGGRSP-LKGLYYKVAADFIIHNYFFSAPEDVLPKSV--TLQEVPAVAVFKDGTYYVYNEEH-------------DGNLSAWVDQERFRCYFQMDSFTLY-------------QMGDRTKLVALTVLEEKNPSADSIRYKSLMEKVATEYRDHYKTDFQFGYMHGNDYINGFIMGELEIPSLIVM---NMTIDGYYLPES----KIETTEE--LLQFLSSVLDGQSQLLGGNGFLRRI 351          
BLAST of SMED30003142 vs. Ensembl Cavefish
Match: pdia3 (protein disulfide isomerase family A member 3 [Source:NCBI gene;Acc:103022595])

HSP 1 Score: 90.5077 bits (223), Expect = 8.351e-19
Identity = 64/237 (27.00%), Postives = 116/237 (48.95%), Query Frame = -1
Query:  985 NVIELNSNFIE--IADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSL--SDTENGTILVWKPDRKLSFHFKE------EISSLNHSLQNWISFNRFSTFPKISQ 1662
            +VIE   +  E  I D    LV+F+APWCG+C++L P Y      +K + + +A++D T   +V S Y + GYPT+K + +G +   +DG RT + ++   K+  GPA            ++E   +  F  +IGDR + +   + D  ++   +S F    +       ++H +L   L  SD +   +++++P R L+  F+E      E    N  ++ +I  N F   P +++
Sbjct:   18 DVIEFTDDDFENKIGDHDLILVEFFAPWCGHCKRLAPEYEAAATRLKGI-VSLAKVDCTANSNVCSKYGVSGYPTLKIFRDGEESGAYDGPRTADGIVSHLKKQAGPA------------SVELKTVADFEKFIGDRDASVVGFFADGGSA--AQSEFLKSASALRESYRFAHTNLEDLLKKSDIDGEGVVLFRPPR-LNNKFEESSVKFSEDKFTNAKIKRFIQDNIFGMCPHMTE 238          

HSP 2 Score: 67.3958 bits (163), Expect = 1.861e-11
Identity = 39/103 (37.86%), Postives = 58/103 (56.31%), Query Frame = -1
Query: 1363 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDATRYPDVASFYQIRGYPTIKYI---NGTDIYTHDGDRTVEHLILFSKR 1659
            V+  N + I   D    L++FYAPWCG+C+ LEP Y EL   + + P + IA++DAT   DV S Y +RG+PTI +       +   ++G R V   I + K+
Sbjct:  370 VVAENFDSIVNDDSKDVLIEFYAPWCGHCKSLEPKYKELGEKLANDPNVVIAKMDATAN-DVPSSYDVRGFPTIYFSPAGQKQNPKRYEGGREVSDFISYLKK 471          
BLAST of SMED30003142 vs. Ensembl Cavefish
Match: pdia4 (protein disulfide isomerase family A member 4 [Source:NCBI gene;Acc:103026638])

HSP 1 Score: 77.7962 bits (190), Expect = 1.403e-14
Identity = 35/94 (37.23%), Postives = 57/94 (60.64%), Query Frame = -1
Query: 1333 LVKFYAPWCGYCRKLEPIYNELEAHIK--DLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLP 1608
            LV+FYAPWCG+C++  P Y ++   +K  D PI +A++DAT+   + S +++ GYPTIK +   +   +DGDR+ + ++   K    P  K  P
Sbjct:   82 LVEFYAPWCGHCKQFAPEYEKIAQVLKESDPPIPVAKVDATKATALGSRFEVSGYPTIKILKKGEPVEYDGDRSEKAIVERVKEVAQPDWKPPP 175          

HSP 2 Score: 77.7962 bits (190), Expect = 1.517e-14
Identity = 57/212 (26.89%), Postives = 101/212 (47.64%), Query Frame = -1
Query: 1000 LVKFYAPWCGYCRKLEPIYNELEAHI--KDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYD---DLANSLR----LRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTF 1608
            LV+FYAPWCG+C++L P Y +    +  +  PI +A++DAT   D+AS + + GYPT+K      ++ ++G R    ++ +     GP  K + +  +  E ++        L +G  SS   AVY+   +  N+LR     R  F S   +F+  S                G +++ +P++   F  K E SS + ++++  S +    F
Sbjct:  197 LVEFYAPWCGHCKRLAPEYEKAAKELSNRTPPILLAKVDATVENDLASRFDVTGYPTLKMFRKGKVFDYNGPREKIGIVDYMSEQAGPPSKQVQALKQIQELLKDG---DDALIVGVFSSEEDAVYEIYQEACNTLRDDYKFRHTFSSEIVKFLKAS---------------PGQVVMLQPEK---FRSKHEPSSYSFTIKDSTSASELQDF 387          

HSP 3 Score: 56.6102 bits (135), Expect = 6.648e-8
Identity = 32/84 (38.10%), Postives = 46/84 (54.76%), Query Frame = -1
Query: 1372 LVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDATRYPDVASFYQIRGYPTIKYINGTD----IYTHDGDRTVEHLILF 1608
            L++FYAPWCG+C+KLEP Y  L    K+   + IA++DAT        Y++ G+PTI +    +    I    G+R VE    F
Sbjct:  545 LIEFYAPWCGHCKKLEPDYLALAKKYKNEKNLVIAKMDATANDVPHDSYKVEGFPTIYFAPSNNKQNPIKFEGGERNVEGFSKF 628          
BLAST of SMED30003142 vs. Ensembl Cavefish
Match: PDIA6 (protein disulfide-isomerase A6 [Source:NCBI gene;Acc:103027650])

HSP 1 Score: 77.0258 bits (188), Expect = 1.577e-14
Identity = 73/262 (27.86%), Postives = 118/262 (45.04%), Query Frame = -1
Query:  973 NVIEL-NSNFIEIADKGP--WLVKFYAPWCGYCRKLEPIYNELEAHIKDL---PIKIARIDATRYPDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLI-----LFSKRALGPAVKHLPSEGRFHE----------AIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENG-TILVWKPDRKLSFH-FKEEISSLN-HSLQNWISFNRFST-------FPKISQHDLW 1662
            +V+EL + NF  +   G   W+V+F+APWCG+C++LEP +      +KD     +K+  +DAT +  +AS Y IRG+PTIK +  G +   ++G RT   ++     LFS  A  P +  +  +  F            A+  H+++T       R+SYL  V   +A   + + + +      +     + +DL SSL     G   +     RK+ +   K   S    H     +S  R ST        PKIS  + W
Sbjct:  162 DVVELTDDNFDRLVLNGDEVWMVEFFAPWCGHCKRLEPEWTAAATAVKDQTKGKVKLGAVDATVHQGLASRYGIRGFPTIKIFSKGEEPEDYEGGRTRADIVARAMDLFSANAPPPELHEIIDQDIFKSTCDDSQLCIVAVLPHILDT---GASGRNSYLD-VMRKMAEKYKKKMWGW------LWAEAGAQMDLESSLGIGGFGYPAMAAINARKMKYSLLKGSFSETGIHEFLRDLSVGRGSTASIGGGALPKISTVEAW 413          

HSP 2 Score: 75.485 bits (184), Expect = 4.152e-14
Identity = 43/112 (38.39%), Postives = 64/112 (57.14%), Query Frame = -1
Query: 1342 NVIELN-SNFIE--IADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHD--GDRTVEHLILFSKRALGPAVK 1662
            +VIELN SNF +  +     WL++FYAPWCG+C++L P + +    +K + +KI  +DA ++  + S Y IRG+PTIK          D  G RT E ++  +  AL   VK
Sbjct:   25 DVIELNPSNFNKQVLQSDSLWLIEFYAPWCGHCQRLTPDWKKAATALKGI-VKIGAVDADQHKSLGSQYGIRGFPTIKVFGANKNKPDDYQGGRTSEAIVEGAMTALRSLVK 135          
BLAST of SMED30003142 vs. Ensembl Sea Lamprey
Match: TMX3 (thioredoxin related transmembrane protein 3 [Source:HGNC Symbol;Acc:HGNC:24718])

HSP 1 Score: 174.866 bits (442), Expect = 8.735e-49
Identity = 117/417 (28.06%), Postives = 201/417 (48.20%), Query Frame = -1
Query:  418 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIK--DLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIAS-MNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDAD 1659
            + +LNS F E  + G WLV FYAPWCG+C+KLEP++NE+   +K    P+++ ++DAT +  +A+ Y +RGYPTIK I G   Y + G RT + +I F+ R  GPA++HL +   F      H  + FFLY+G  S  LK  +  +A  + + +YF+S   + +P + + H          E    LV+K        + E   S +  L  W+   RF TF  I  + ++ +    K +++ I +                 E +  +L    + +A E       D  F +       M++L M+++  P++L+F   +   Y+  +         +  + +  F+++   G +  +GG G   K++R  ++    +      +P+LA  + G+PL VIS +CY    M T+D D
Sbjct:    1 ISDLNSRFAEHKNDGVWLVDFYAPWCGHCKKLEPVWNEVAQEMKASGSPVRVGKLDATAHSGIATEYGVRGYPTIKLIKGDLSYNYKGPRTKDEIIAFANRVAGPAMRHLSTPALFAHTQSRH--DIFFLYVGGDSK-LKESFTQVALEMVVNAYFFSTPEEVLPANVHIH----------EAPAALVFKDGT-----YYEYDESDDGDLATWVRRERFPTFQAIDGYAMYAISDTGKLVVLAIVDPMST-------------EENHSRLKKLVEDVAHEYREHFHRDFQFGYVDGAEGYMSNLLMSDVEAPSLLVFNTSSQEYYLPTE-------PLLSNQDLLSFINNILDGSVPAHGGDGLGQKIKRMLFELKATITSVFRASPLLACVLFGLPLGVISTMCY---WMWTADGD 376          
BLAST of SMED30003142 vs. Ensembl Sea Lamprey
Match: TMX3 (thioredoxin related transmembrane protein 3 [Source:HGNC Symbol;Acc:HGNC:24718])

HSP 1 Score: 143.665 bits (361), Expect = 6.973e-38
Identity = 108/408 (26.47%), Postives = 198/408 (48.53%), Query Frame = -1
Query:  451 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNE--LEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTF---PKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCY 1659
            ++EL S   ++  + PWLV FYAPWC +C+KL+P++ +  L  H    P+++ ++DA ++  +AS +  RG+P+IK I G   Y + G  + E +I F+ R  GP V  L S   F +A ++H  +T F+Y+G  S  LK  Y ++A  L + +YFYS   + +P+    +          E   +L +K +   +F+  +E    + SL+ W++  RF  F     I+ HDL +        ++   N    + +          E S     +F K+  F             ++     +NDL M+ + VP+V++    +   ++ P+ +    +++  + F   F+ S  +G++E +GG     +M+R  YD  T +      + +L +F+ G P+ V + +CY
Sbjct:   27 IVELTSTLHDLRQQEPWLVNFYAPWCSHCKKLDPVWRDVALGLHEAGSPVRVGKMDAIKHSSLASEFGARGFPSIKLIKGDLAYNYRGPHSKEKIIEFANRVSGPTVLSLSSPEAFEKAQKAH--DTCFVYVGGESP-LKEKYLEVATELIVYTYFYSATKEALPEDVILY----------ELPAVLAFKDE---TFYVFDEFE--DGSLEEWVNRERFPEFLFIDSITLHDLADTGKSLVLAVLSGTNIKHDNRLKYMV-----EEVSKNYRRNFHKQFQFG------------YTDGNIHINDLIMSVVDVPSVILVNTSSQQFFL-PEGD----LRSAKDLFT--FITSNLAGKLEAHGGNSPYFRMKRLLYDASTVISAVFQQSVLLGLFLYGTPVVVFTLICY 392          
BLAST of SMED30003142 vs. Ensembl Sea Lamprey
Match: pdia4 (protein disulfide isomerase family A, member 4 [Source:ZFIN;Acc:ZDB-GENE-030131-5493])

HSP 1 Score: 74.7146 bits (182), Expect = 2.400e-14
Identity = 39/109 (35.78%), Postives = 60/109 (55.05%), Query Frame = -1
Query: 1333 ELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIK--DLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLP 1653
            +LN +F  +A K   L++FYAPWCG+C++    Y ++   +   D PI +A++DAT    +A  + + GYPTIK +       +DGDRT + +I   K    P  K  P
Sbjct:   63 DLNFDFF-VAGKDTVLLEFYAPWCGHCKEFASEYEKIAKELAENDPPIPVAKVDATVAVHLAERFSVSGYPTIKILKKGQAVDYDGDRTQDAIIAKVKEVASPDWKPPP 170          

HSP 2 Score: 72.4034 bits (176), Expect = 1.293e-13
Identity = 39/108 (36.11%), Postives = 57/108 (52.78%), Query Frame = -1
Query: 1330 SNFIEIA-DKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKD--LPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPS 1644
            SNF EI  D    LV+FYAPWCG+C+KL P Y +    +     PI +A++DAT   ++   + + GYPT+K       Y ++G R    ++       GPA + L S
Sbjct:  179 SNFDEIVNDADIILVEFYAPWCGHCKKLAPEYEKAAQELSKNTPPIPLAKVDATVESELGKRFGVSGYPTLKLFRKGKAYDYNGPREQYGIVSHMIEQAGPASQELKS 286          

HSP 3 Score: 57.7658 bits (138), Expect = 5.824e-9
Identity = 33/87 (37.93%), Postives = 47/87 (54.02%), Query Frame = -1
Query: 1363 LVKFYAPWCGYCRKLEPIYNELEAHIKD-LPIKIARIDATRYPDVASFYQIRGYPTIKY----INGTDIYTHDGDRTVEHLILFSKR 1608
            L++FYAPWCG+C+ LEPIY +L    K    + IA++DAT        Y+  G+PTI +         I   DG R +E L  F ++
Sbjct:  542 LIEFYAPWCGHCKSLEPIYKDLGKKYKGHKGLVIAKMDATVNDIPHDSYKAEGFPTIYFSPSGAKDRPIKFADGSRDLESLSKFIEK 628          
BLAST of SMED30003142 vs. Ensembl Sea Lamprey
Match: pdia3 (protein disulfide isomerase family A, member 3 [Source:ZFIN;Acc:ZDB-GENE-031002-9])

HSP 1 Score: 73.1738 bits (178), Expect = 6.115e-14
Identity = 45/185 (24.32%), Postives = 95/185 (51.35%), Query Frame = -1
Query: 1063 LVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYT-HDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENG--TILVWKPDRKLSFHFKE 1608
            LV+F+APWCG+C++L P Y +    +K+  + +A++D T   +  + + + GYPT+K     ++ + +DG RT + ++ F K+  GP+            ++E   +E    ++GD    +   + D ++    ++ F  + +       ++H ++ + L D +     I+V++P R L+  F+E
Sbjct:   46 LVEFFAPWCGHCKRLAPEYEKAATKLKNT-VALAKVDCTANTETCNKFGVSGYPTLKIFRDGELSSDYDGPRTADGIVGFLKKQAGPS------------SVELKTVEDLEKFVGDNDPSIVGFFKDASSD--AQTEFMKVASSQRDNYRFAHSNVQAVL-DKQGAEEAIVVFRPLR-LASKFEE 213          

HSP 2 Score: 67.781 bits (164), Expect = 3.038e-12
Identity = 38/86 (44.19%), Postives = 54/86 (62.79%), Query Frame = -1
Query: 1363 LVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDATRYPDVASFYQIRGYPTIKY--INGTDI-YTHDGDRTVEHLILFSKR 1608
            L++FYAPWCG+C+ LEP Y EL   +K+ P I IA++DAT   DV + Y +RG+PTI +  +   D    ++G R V   I + KR
Sbjct:  394 LIEFYAPWCGHCKNLEPKYLELADKLKNDPNILIAKMDATAN-DVPAQYDVRGFPTIYFSPMGSKDSPKKYEGGREVSDFISYLKR 478          
BLAST of SMED30003142 vs. Ensembl Sea Lamprey
Match: pdia6 (protein disulfide isomerase family A, member 6 [Source:ZFIN;Acc:ZDB-GENE-030131-879])

HSP 1 Score: 70.4774 bits (171), Expect = 3.804e-13
Identity = 41/112 (36.61%), Postives = 63/112 (56.25%), Query Frame = -1
Query: 1342 NVIELN-SNFIE--IADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKY--INGTDIYTHDGDRTVEHLILFSKRALGPAVK 1662
            +VIEL  SNF    +     WLV+FYAPWCG+C++L P + +    +K + +K+  +DA  +  + S Y +RG+PTIK   IN       +G RT + ++  +  AL   VK
Sbjct:   26 DVIELTASNFNREVVQSNDLWLVEFYAPWCGHCQRLIPEWKKAATALKGI-VKLGAVDADEHKGLGSTYNVRGFPTIKIFGINKNKPEDFNGGRTTQGIVDGALGALRSLVK 136          

HSP 2 Score: 68.9366 bits (167), Expect = 1.219e-12
Identity = 39/130 (30.00%), Postives = 70/130 (53.85%), Query Frame = -1
Query: 1291 LNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIK---DLPIKIARIDATRYPDVASFYQIRGYPTIK-YINGTD-IYTHDGDRTVEHLI-----LFSKRALGPAVKHLPSEGRFHEAIESHLM 1650
            + +N I       W+V+F+APWCG+C+KLEP +      +K   +  +K+A +DAT   ++++ + IRG+PTIK +  G D    + G RT   ++     L++  A  P V  + ++    ++ E H +
Sbjct:  171 ITTNKITFTPSETWIVEFFAPWCGHCKKLEPEWAAAATEVKAQTNGRVKLAAVDATVNQELSNRFGIRGFPTIKVFRKGEDEPQEYQGGRTKSDIVAYALELYTDNAPAPEVVEILNQDVVKKSCEEHQL 300          
BLAST of SMED30003142 vs. Ensembl Yeast
Match: PDI1 (Protein disulfide isomerase; multifunctional oxidoreductase of the ER lumen, essential for disulfide bond formation in secretory and cell-surface proteins, processing of non-native disulfide bonds; Ero1p activator; complexes with exomannosidase, Mnl1p to facilitate the recognition of misfolded glycoproteins and the trimming of glycan Man8GlcNAc2 to Man7GlcNAc2 on substrates, thereby accelerating ERAD; PDI1 has a paralog, EUG1, that arose from the whole genome duplication [Source:SGD;Acc:S000000548])

HSP 1 Score: 64.3142 bits (155), Expect = 2.872e-11
Identity = 29/91 (31.87%), Postives = 51/91 (56.04%), Query Frame = -1
Query: 1345 LVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDI---YTHDGDRTVEHLILFSKRALGPAV 1608
            L +F+APWCG+C+ + P Y +    + +  I +A+ID T   D+   + I G+P++K    +D+     ++G RT E ++ F  +   PAV
Sbjct:   53 LAEFFAPWCGHCKNMAPEYVKAAETLVEKNITLAQIDCTENQDLCMEHNIPGFPSLKIFKNSDVNNSIDYEGPRTAEAIVQFMIKQSQPAV 143          

HSP 2 Score: 51.9878 bits (123), Expect = 1.806e-7
Identity = 32/87 (36.78%), Postives = 46/87 (52.87%), Query Frame = -1
Query: 1363 LVKFYAPWCGYCRKLEPIYNELEAHIKDLP--IKIARIDATRYPDVASFYQIRGYPTIKYING---TDIYTHDGDRTVEHLILFSKR 1608
            LV +YAPWCG+C++L P Y EL     +    + IA++D T   DV     I GYPTI    G   ++   + G R+++ L  F K 
Sbjct:  398 LVLYYAPWCGHCKRLAPTYQELADTYANATSDVLIAKLDHTEN-DVRGVV-IEGYPTIVLYPGGKKSESVVYQGSRSLDSLFDFIKE 482          
BLAST of SMED30003142 vs. Ensembl Yeast
Match: EPS1 (ER protein with chaperone and co-chaperone activity; involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family [Source:SGD;Acc:S000001267])

HSP 1 Score: 47.7506 bits (112), Expect = 4.632e-6
Identity = 28/100 (28.00%), Postives = 49/100 (49.00%), Query Frame = -1
Query: 1363 SNFIEIADKGPWLVKFYAPWCGYCRKLEPI----YNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTH--DGDRTVEHLILFSKR 1644
            +NF E   KG  ++ FY+P+C +C+ L P+    + E +   K L I  ++++     D+     I  +P I+  N +       +  RT E LI F++R
Sbjct:   40 TNFKEELSKGLHIIDFYSPYCPHCKHLAPVWMETWEEFKEESKTLNITFSQVNCIESADLCGDENIEYFPEIRLYNPSGYIKSFTETPRTKESLIAFARR 139          
BLAST of SMED30003142 vs. Ensembl Nematostella
Match: EDO41581 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S406])

HSP 1 Score: 154.836 bits (390), Expect = 6.308e-43
Identity = 78/230 (33.91%), Postives = 134/230 (58.26%), Query Frame = -1
Query:  976 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSY--LKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDL 1659
            V+EL+  F  + ++G WLV+FYAPWCGYCRKLEP+Y E+   +    I +A++DAT Y  ++  Y +RG+PTIK+I G  +  ++GDRT + +I F+++A GPAV+ L S     +      +  FFL +        LK  YD  A+ +  ++YFYSI++  +P+S    + +N+         I V+K  R   +   +++++ N  + +W+S  +F  F +I++ ++
Sbjct:   27 VVELDDRFAHLKEQGSWLVEFYAPWCGYCRKLEPVYEEVAKTLHGSSINVAKLDATVYSGISREYGVRGFPTIKFIKGKKVINYEGDRTAQDIIQFAQKASGPAVRELTSGEELRKVQRERPV--FFLLVQKSGEIDTLKDHYDKAADMMLTKAYFYSIDDNQLPKS----IKINAP-------AISVFKDGRHFEYEVPDDVAASN--ISDWVSQEQFPAFIQITRTNI 241          
BLAST of SMED30003142 vs. Ensembl Nematostella
Match: EDO31634 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SXD7])

HSP 1 Score: 78.1814 bits (191), Expect = 2.996e-15
Identity = 56/162 (34.57%), Postives = 86/162 (53.09%), Query Frame = -1
Query: 1213 NVIEL-NSNFIE-IADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIK--DLPIKIARIDATRY-PDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLILFSKRALGP------AVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLR 1662
            +V++L +SNF   +A K   LV+F+APWCG+C++L P Y      +K  D P+ +A++D T    D  S Y + GYPT+K + NG     +DG R    +I + K+  GP      +V HL  E +  +A ES+++  F     D    LK  +   AN +R
Sbjct:   19 DVLDLGDSNFKSGVAGKDIMLVEFFAPWCGHCKRLAPEYETAAEALKKNDPPVPLAKVDCTEAGKDTCSKYGVSGYPTLKIFRNGEMSKDYDGPRDSSGIIRYMKKQAGPSSVEIKSVDHL--EKKLDDA-ESNVVVGFL----DGDDDLKNAFMRTANEMR 173          

HSP 2 Score: 68.1662 bits (165), Expect = 3.801e-12
Identity = 39/104 (37.50%), Postives = 60/104 (57.69%), Query Frame = -1
Query: 1363 IELNSNFIEIADK--GPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDATRYPDVASFYQIRGYPTIKYI---NGTDIYTHDGDRTVEHLILFSKR 1656
            + +  NF EI +      L++FYAPWCG+C+ LEP YNEL   ++D+  I IA++DAT   D    + ++G+PTI +    N  +   ++G R V   + F KR
Sbjct:  365 VVVGENFKEIVNDPTKDVLIEFYAPWCGHCKSLEPKYNELGEKLQDVKDIVIAKMDATAN-DAPPNFSVQGFPTIYWAPANNKENPEKYEGGREVSDFVDFIKR 467          
BLAST of SMED30003142 vs. Ensembl Nematostella
Match: EDO41377 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4E7])

HSP 1 Score: 75.8702 bits (185), Expect = 7.568e-15
Identity = 34/81 (41.98%), Postives = 51/81 (62.96%), Query Frame = -1
Query: 1372 LVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKI-ARIDATRYPDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLILF 1608
            LV FYAPWCG+C+  +P Y +     KD P ++ A++D T++ DV    ++ GYPT++ Y+ G  +  +DGDR  E LI F
Sbjct:  260 LVMFYAPWCGHCKNAKPKYEKAAETFKDQPNRVFAKLDCTKFGDVCDKEEVNGYPTLRYYLYGKFVVEYDGDRVTEDLISF 340          

HSP 2 Score: 69.707 bits (169), Expect = 7.849e-13
Identity = 33/86 (38.37%), Postives = 53/86 (61.63%), Query Frame = -1
Query: 1360 LVKFYAPWCGYCRKLEPIYNELEA--HIKDLPIKIARIDATRYPDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLILFSKRA 1608
            LV ++APWCG+C +++P Y +     H +D    +A +D T++ DVA    + GYPT+K Y NG     ++GDR+ + L+LF + A
Sbjct:  140 LVMYFAPWCGHCNEMKPNYYKAAQVLHDEDANCNLAAVDCTKHKDVAKKVALAGYPTVKLYKNGKVAKEYEGDRSEKDLVLFMRTA 225          

HSP 3 Score: 48.9062 bits (115), Expect = 3.350e-6
Identity = 23/73 (31.51%), Postives = 42/73 (57.53%), Query Frame = -1
Query: 1372 CGYCRKLEPIYNELEAHI-KDLPIKIARIDATRYPDVASFYQIRGYPTIKYI-NGTDIYTHDGDRTVEHLILF 1584
            C +C+K++P++ +    + KD+   +A +D T   +  +   I+GYPT++YI  G   + + G RT E L+ F
Sbjct:   26 CPHCQKMKPVFEKAAKQLGKDVKGALAAVDCTESKNTCNQRDIKGYPTLQYIREGEFQFKYTGRRTAEALVSF 98          
BLAST of SMED30003142 vs. Ensembl Nematostella
Match: EDO36830 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SHH5])

HSP 1 Score: 76.6406 bits (187), Expect = 9.652e-15
Identity = 34/95 (35.79%), Postives = 53/95 (55.79%), Query Frame = -1
Query: 1330 LVKFYAPWCGYCRKLEPIYNELEAHIKDLP--IKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPS 1608
            LV+FYAPWCG+C+ L P Y +    +K     IK+A++DAT    +   +Q++GYPTIK+        + G RT   ++ +  +  GP  K L +
Sbjct:   45 LVEFYAPWCGHCKALAPEYAKAAGQLKSEKSEIKLAKVDATAETKLGEKFQVQGYPTIKFFKDGKPSEYAGGRTAPEIVSWLNKKTGPPAKDLAT 139          

HSP 2 Score: 59.6918 bits (143), Expect = 2.204e-9
Identity = 38/114 (33.33%), Postives = 63/114 (55.26%), Query Frame = -1
Query: 1321 NSNFIEIA---DKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKD-LPIKIARIDATRYPDVASFYQIRGYPTIKYI-NGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGR 1647
              NF E+A   DK  + V+FYAPWCG+C++L PI+++L    KD   I +A++D+T   +     ++  +PTIKY     +   ++G RT++  + F +   G A     +EG 
Sbjct:  371 GKNFDEVARNKDKNVF-VEFYAPWCGHCKQLAPIWDQLGEKYKDHADIVVAKMDST--ANEVEGVKVHSFPTIKYFPKEGEAVDYNGGRTLDDFVKFLESG-GKAGNEPAAEGE 480          
BLAST of SMED30003142 vs. Ensembl Nematostella
Match: EDO33843 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SR22])

HSP 1 Score: 70.0922 bits (170), Expect = 2.561e-14
Identity = 41/101 (40.59%), Postives = 57/101 (56.44%), Query Frame = -1
Query: 1372 IELNSNFIEIAD--KGPWLVKFYAPWCGYCRKLEPIYNELEAHIK-DLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTH---DGDRTVEHLILF 1656
            + +   F EI +  K   L++FYAPWCG+C+ LEP + +L  H + D  I IA+IDAT   DV S Y + G+PTI +    D       DG R ++ LI F
Sbjct:   31 VVVGKTFDEIVNDPKKDVLIEFYAPWCGHCKALEPTFKKLGKHFRNDKNIVIAKIDATAN-DVPSTYAVEGFPTIYFATSKDKKNPIKFDGGRELKDLIKF 130          
BLAST of SMED30003142 vs. Ensembl Medaka
Match: TMX3 (thioredoxin related transmembrane protein 3 [Source:NCBI gene;Acc:101165851])

HSP 1 Score: 191.045 bits (484), Expect = 1.605e-53
Identity = 126/418 (30.14%), Postives = 217/418 (51.91%), Query Frame = -1
Query:  415 VIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDL--PIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIAS-MNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADN 1659
            V +L+S F +      WLV FYAPWCGYC+KLEPI++E+ A ++    P+++ ++DAT Y  +AS + +RGYPTIK +     Y + G RT + ++ F+ R  GPAV+ LPS   F   ++ H  +  F+Y+G  S  LK  Y+D A+ L + +YF+S     +P+          S+S  E   ++V+K     ++   E     + SL +W++  RF  + +I    L+EL    K + I + +    +            ES  +K  +  +++A E + +  N +F     D +  +N L M  + VP+V+I    N   ++   S   + M+ +  +F+ D L+    G  + YGG GF  +++R  +D  + ++    ++P+L  F+ G+PL VIS +CY IC  ++ D  +
Sbjct:   44 VEDLDSTFKDTRVNDIWLVDFYAPWCGYCKKLEPIWDEVGAELRSSGSPVRVGKMDATAYSGMASEFGVRGYPTIKLLKADLAYNYKGPRTKDDIVEFANRVAGPAVRALPSTQMFEHMMKRH--DVLFVYVGGDSP-LKEKYNDAASELIVYTYFFSASEDAVPE----------SISMPELPAVVVFKDGDYFTYDEYE-----DGSLSSWVNRERFQGYLQIDGFTLYELGETGKLVAIAVIDEKDAT-----------EESGRLK--TLMQRVAKE-YREHFNRVFQFGHMDGSDYINSLIMGEVKVPSVIILNTSNEQYFLS--SEPIETMEQL-VQFISDVLN----GSAQAYGGDGFIQRIKRVAFDARSTIMSVFRSSPLLGCFLFGLPLGVISLMCYGICTAESDDGSD 422          
BLAST of SMED30003142 vs. Ensembl Medaka
Match: ENSORLT00000009634.2 (protein disulfide-isomerase TMX3-like [Source:NCBI gene;Acc:101157158])

HSP 1 Score: 140.969 bits (354), Expect = 6.309e-36
Identity = 118/444 (26.58%), Postives = 208/444 (46.85%), Query Frame = -1
Query:  379 VIELNSNFIEIADKGP---WLVKFYAPWCGYCRKLEPIYNELEAHIKDLP--IKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERE--SSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIAS--MNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAID-EKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEAR-------QQKKIR 1659
            V EL+ +F+E   KG    WL++FYAPWC +C++L+P+++E+ + ++ L   +++ + DAT    +A  +++R YP I  +     Y + G RT E ++ F+ R  GP V+ L S   F  A+  H     F+Y+G  +S LK  +  +A  L + ++F+S     +P+S    LD   +        I+V+K    L++   +E S  +  L+ WI+  RF  F  I  + L+               +   S      +   ER   S +++     +++A E  + E    FF++    A   +N   M  + VP+++     N           YQ    ++ E+ + DFL     G IE  GG GF  ++QR  Y+    +I      P++  F+ G PL++ +F C+ +CC + +  D+    A        Q+KKI+
Sbjct:   24 VEELDDSFMET--KGSDEIWLIEFYAPWCSFCKQLDPVWHEIGSELRSLGSMVQVGKSDATVSTGLAKEFRVRTYPAILMLKKNTKYNYAGPRTKEGIMDFANRMEGPVVRSLSSPQLFQHAMSHH--NVMFVYVG-ATSELKGNFTTVAEELIVHTHFFSTTRDVLPKSV--SLDYLPA--------IVVFKDRTYLTY---DEES--DGDLKAWINRERFLNFQHIDSYTLY---------------AMGESGKLVLLALVEERHLCSKSLRYKHLVERVAEE--HKETYSRFFLFGFMAAGDYINGFVMGEVTVPSLIAVNLSN--------DGYYQPAGPVETERQLLDFLDGVLDGTIEMKGGNGFSQRVQRVIYETKATVIPVFRDAPLIGCFLAGFPLAIGAFFCF-LCCRNQTIPDDDEDTAALLASSVPQRKKIK 421          
BLAST of SMED30003142 vs. Ensembl Medaka
Match: pdia3 (protein disulfide isomerase family A member 3 [Source:NCBI gene;Acc:101164975])

HSP 1 Score: 79.337 bits (194), Expect = 3.900e-15
Identity = 53/217 (24.42%), Postives = 104/217 (47.93%), Query Frame = -1
Query:  988 LVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDT---ENGTILVWKPDRKLSFHFKE------EISSLNHSLQNWISFNRFSTFPKIS 1608
            LV+F+APWCG+C++L P Y      +K + + + ++D T   ++ S Y + GYPT+K + +G +   +DG R+ + ++ F K+  GPA   L ++  F +            +IGD+ + +   + D + S   ++ F    +       ++H +  + L      E G +L   P  +L+  F+E      E    ++ ++ +I  N F   P ++
Sbjct:   38 LVEFFAPWCGHCKRLAPEYEAAATRLKGI-VPLVKVDCTANSNICSKYGVSGYPTLKIFRDGEESGPYDGPRSADGIVSFLKKQAGPASVELKTDADFEK------------FIGDQDASVVGFFADQSTS---QAEFLKAASALRDDYRFAHTNSEALLKSNGIDEEGVVLFRPP--RLNNKFEENSVKFTEDKFTSNKIKKFIQENIFGICPHMT 236          

HSP 2 Score: 70.4774 bits (171), Expect = 1.971e-12
Identity = 37/86 (43.02%), Postives = 53/86 (61.63%), Query Frame = -1
Query: 1363 LVKFYAPWCGYCRKLEPIYNELEAHIKDLP-IKIARIDATRYPDVASFYQIRGYPTIKYING---TDIYTHDGDRTVEHLILFSKR 1608
            L++FYAPWCG+C+ LEP YNEL   + + P + IA++DAT   DV S Y++ G+PTI +      T    ++G R V   I + KR
Sbjct:  386 LIEFYAPWCGHCKNLEPKYNELGEKLANDPNVVIAKMDATAN-DVPSPYEVSGFPTIYFSPAGRKTSPKKYEGGREVSDFISYLKR 470          
BLAST of SMED30003142 vs. Ensembl Medaka
Match: pdia2 (protein disulfide-isomerase [Source:NCBI gene;Acc:101164281])

HSP 1 Score: 79.337 bits (194), Expect = 4.043e-15
Identity = 57/204 (27.94%), Postives = 97/204 (47.55%), Query Frame = -1
Query: 1015 LVKFYAPWCGYCRKLEPIYNELEAHIKD--LPIKIARIDATRYPDVASFYQIRGYPTIK-YING--TDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWK-PDRKLSFHFKEEISSLNHSLQNWISFN 1608
            +V+FYAPWCGYCR+ EPIY E    +K+    +++A++DA    ++A  + +  +PT+K ++NG   +   + G RT   ++ + KR  GP    L S     E I +H + +   +  +  S   AV+ ++A  L    +  +      P+ +  +         T N  +L  K  D +  F   EE     H+L N+I  N
Sbjct:   90 MVEFYAPWCGYCRRFEPIYAEAAGMLKEEGSEMRLAKVDAIEEKELAEEFNVDSFPTVKLFMNGDRKEPIEYTGKRTPSGIVQWLKRRSGPGAADLNSADSAAEFINTHNV-SVVGFFDNLESEAAAVFKEVAFDLTDVEFAVTAS----PEVFKEY-------EVTANKVVLFKKFDDGRADFELSEEDKLDKHNLTNFIKEN 281          

HSP 2 Score: 59.3066 bits (142), Expect = 8.526e-9
Identity = 32/81 (39.51%), Postives = 49/81 (60.49%), Query Frame = -1
Query: 1372 VKFYAPWCGYCRKLEPIYNEL-EAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTD--IYTHDGDRTVEHLILF 1605
            V+FYAPWCG+C++L PI+ +L E +     I IA++DAT   +      I G+PT+KY    D  + ++ G+R +E L  F
Sbjct:  422 VEFYAPWCGHCKELAPIWEQLGEIYADHDDIIIAKMDAT--ANEVESVAIDGFPTLKYFPAGDKEVISYTGNRDLETLSKF 500          
BLAST of SMED30003142 vs. Ensembl Medaka
Match: pdia2 (protein disulfide-isomerase [Source:NCBI gene;Acc:101164281])

HSP 1 Score: 78.9518 bits (193), Expect = 4.809e-15
Identity = 57/204 (27.94%), Postives = 97/204 (47.55%), Query Frame = -1
Query: 1015 LVKFYAPWCGYCRKLEPIYNELEAHIKD--LPIKIARIDATRYPDVASFYQIRGYPTIK-YING--TDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWK-PDRKLSFHFKEEISSLNHSLQNWISFN 1608
            +V+FYAPWCGYCR+ EPIY E    +K+    +++A++DA    ++A  + +  +PT+K ++NG   +   + G RT   ++ + KR  GP    L S     E I +H + +   +  +  S   AV+ ++A  L    +  +      P+ +  +         T N  +L  K  D +  F   EE     H+L N+I  N
Sbjct:   86 MVEFYAPWCGYCRRFEPIYAEAAGMLKEEGSEMRLAKVDAIEEKELAEEFNVDSFPTVKLFMNGDRKEPIEYTGKRTPSGIVQWLKRRSGPGAADLNSADSAAEFINTHNV-SVVGFFDNLESEAAAVFKEVAFDLTDVEFAVTAS----PEVFKEY-------EVTANKVVLFKKFDDGRADFELSEEDKLDKHNLTNFIKEN 277          

HSP 2 Score: 61.2326 bits (147), Expect = 1.990e-9
Identity = 34/80 (42.50%), Postives = 49/80 (61.25%), Query Frame = -1
Query: 1372 LVKFYAPWCGYCRKLEPIYNEL-EAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILF 1608
             V+FYAPWCG+C++L PI+ +L E +     I IA++DAT   +V S   I G+PT+KY    D   + G+R +E L  F
Sbjct:  431 FVEFYAPWCGHCKELAPIWEQLGEIYADHDDIIIAKMDATAN-EVESV-AIDGFPTLKYFPAGDKEVYTGNRDLETLSKF 508          
BLAST of SMED30003142 vs. Planmine SMEST
Match: SMESG000029028.1 (SMESG000029028.1)

HSP 1 Score: 861.677 bits (2225), Expect = 0.000e+0
Identity = 450/453 (99.34%), Postives = 452/453 (99.78%), Query Frame = -1
Query:  304 NVIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPERESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEARQQKKIRIPLYEVALKSSEELNIKSDGDKKNE 1662
            NVIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYS+LDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWELEILKKSLIILICNSSLLSDINSNPSDNPE+ESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFV DFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEARQQKKIRIPLYEVALKSSEELNIKSDGDKKNE
Sbjct:   21 NVIELNSNFIEIADKGPWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSYLDLNSSLSDTENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLWELEILKKSLIILICNSSLLSDINSNPSDNPEQESSTMKLFSFAKKLAFESFNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNNYQKMKAIDEKFVLDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEFVSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEARQQKKIRIPLYEVALKSSEELNIKSDGDKKNE 473          
BLAST of SMED30003142 vs. Planmine SMEST
Match: SMESG000023147.1 (SMESG000023147.1)

HSP 1 Score: 91.6633 bits (226), Expect = 2.902e-19
Identity = 50/146 (34.25%), Postives = 82/146 (56.16%), Query Frame = -1
Query: 1219 NSNFIEIADKGPW-LVKFYAPWCGYCRKLEPIYNELEAHIKDLP--IKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLYIGDRSSYLKAVYDDLANS 1647
            NSNF E+ +K  + LV+FYAPWCG+C+ L P Y      +K+    IK+A++DAT   D+AS ++I+GYPT+K+     +  + G RT + +I + ++  GP    L S       IES  +     +  D++S     ++ +A+S
Sbjct:   31 NSNFNEVIEKNKYVLVEFYAPWCGHCKALAPEYASAAEKLKEKNSIIKLAKVDATVENDLASKFKIQGYPTLKFFKDGKVMDYGGGRTADTIISWLEKKTGPPAATLKSVEEAKALIESKDV-VVVGFFKDQASAAAKAFESVADS 175          

HSP 2 Score: 62.3882 bits (150), Expect = 5.280e-10
Identity = 37/98 (37.76%), Postives = 59/98 (60.20%), Query Frame = -1
Query: 1372 LNSNFIEIA-DKGPWL-VKFYAPWCGYCRKLEPIYNELEAHIKD-LPIKIARIDATRYPDVASFYQIRGYPTIKYI--NGTDIYTHDGDRTVEHLILF 1650
            +  NF  +A DK   + V+FYAPWCG+C++L PI+++L    KD   I IA++D+T   +     +I+ +PTIKY   +  ++   +GDRT+E    F
Sbjct:  371 VGKNFDSVARDKSKAVFVEFYAPWCGHCKQLAPIWDQLGETYKDNTDIVIAKMDST--ANEVEGVRIQSFPTIKYFPKDSDEMVDFEGDRTLETFKKF 466          
BLAST of SMED30003142 vs. Planmine SMEST
Match: SMESG000017020.1 (SMESG000017020.1)

HSP 1 Score: 80.4925 bits (197), Expect = 8.161e-16
Identity = 43/136 (31.62%), Postives = 78/136 (57.35%), Query Frame = -1
Query: 1291 NVIEL-NSNFIEIADKG--PWLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKY----INGTDIYTHDGDRTVEHLILFSKRAL-----GPAVKHLPSEGRFHEAIESHLM 1662
            +V+EL +SNF E+  K   PWLV+F+APWCG+C+ L+P +++  + ++   +K+  +DAT +   AS Y ++GYPTIK+    +   D   +DG R+   ++ ++   +      P +K + ++     A E H +
Sbjct:  161 DVVELTDSNFDEMVLKSEEPWLVEFFAPWCGHCKNLKPHWDQAASELRG-KVKLGALDATVHTATASRYGVQGYPTIKFFPAGVKSGDSQDYDGGRSSSDIVRWASDKVVEQLPAPEIKEILNQEILKSACEDHQL 295          

HSP 2 Score: 67.0106 bits (162), Expect = 1.680e-11
Identity = 34/99 (34.34%), Postives = 56/99 (56.57%), Query Frame = -1
Query: 1378 NVIELN-SNFIEIADKGP-WLVKFYAPWCGYCRKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIYTHD--GDRTVEHLI 1662
            +V+EL  SNF ++ +    W V+FYAPWCG+C+ L P + +    +K + +K+  +D  +Y  +   Y I+G+PTIK    +     D  GDRT   ++
Sbjct:   25 DVVELTPSNFDDMKNSDSLWFVEFYAPWCGHCQSLAPEWKKAATALKGV-VKVGAVDMDKYSSLGQPYGIQGFPTIKIFGKSKHSPKDYQGDRTAGSIV 122          
BLAST of SMED30003142 vs. Planmine SMEST
Match: SMESG000041085.1 (SMESG000041085.1)

HSP 1 Score: 78.9518 bits (193), Expect = 3.114e-15
Identity = 38/102 (37.25%), Postives = 59/102 (57.84%), Query Frame = -1
Query: 1312 LVKFYAPWCGYCRKLEPIYNELEAHIK--DLPIKIARIDATRYPDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHE 1608
            LV+FYAPWCG+C+KL P Y      +K  D PI IA++D T        + + GYPT+K + NG   + + G+R+ + +I +     GP+ K L +E  F++
Sbjct:   38 LVEFYAPWCGHCKKLAPEYEIAATALKKQDPPIAIAKVDCTAETKTCEKHGVSGYPTLKVFKNGQFAFDYSGERSADGIIKYMVSKSGPSSKELKTEENFNK 139          

HSP 2 Score: 61.6178 bits (148), Expect = 8.763e-10
Identity = 33/100 (33.00%), Postives = 55/100 (55.00%), Query Frame = -1
Query: 1321 LVKFYAPWCGYCRKLEPIYNELEAHIK-DLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIY---THDGDRTVEHLILFSKRALGPAVKHLPSEGR 1608
            L++FYAPWCG+C+ L P Y EL   +  +  I IA++DAT   DV S Y++ G+PTI +      +   ++   R V+ ++ +        +K+   +GR
Sbjct:  386 LIEFYAPWCGHCKSLAPKYEELAKKLSAESDIVIAKMDATAN-DVPSPYEVSGFPTIYFAPKNSKHSPKSYSSGREVDDMLKYIASEASSPLKNFDRQGR 484          
BLAST of SMED30003142 vs. Planmine SMEST
Match: SMESG000041085.1 (SMESG000041085.1)

HSP 1 Score: 78.9518 bits (193), Expect = 3.200e-15
Identity = 38/102 (37.25%), Postives = 59/102 (57.84%), Query Frame = -1
Query: 1312 LVKFYAPWCGYCRKLEPIYNELEAHIK--DLPIKIARIDATRYPDVASFYQIRGYPTIK-YINGTDIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHE 1608
            LV+FYAPWCG+C+KL P Y      +K  D PI IA++D T        + + GYPT+K + NG   + + G+R+ + +I +     GP+ K L +E  F++
Sbjct:   24 LVEFYAPWCGHCKKLAPEYEIAATALKKQDPPIAIAKVDCTAETKTCEKHGVSGYPTLKVFKNGQFAFDYSGERSADGIIKYMVSKSGPSSKELKTEENFNK 125          

HSP 2 Score: 61.6178 bits (148), Expect = 9.876e-10
Identity = 33/100 (33.00%), Postives = 55/100 (55.00%), Query Frame = -1
Query: 1321 LVKFYAPWCGYCRKLEPIYNELEAHIK-DLPIKIARIDATRYPDVASFYQIRGYPTIKYINGTDIY---THDGDRTVEHLILFSKRALGPAVKHLPSEGR 1608
            L++FYAPWCG+C+ L P Y EL   +  +  I IA++DAT   DV S Y++ G+PTI +      +   ++   R V+ ++ +        +K+   +GR
Sbjct:  372 LIEFYAPWCGHCKSLAPKYEELAKKLSAESDIVIAKMDATAN-DVPSPYEVSGFPTIYFAPKNSKHSPKSYSSGREVDDMLKYIASEASSPLKNFDRQGR 470          
The following BLAST results are available for this feature:
BLAST of SMED30003142 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TMX39.316e-4728.24thioredoxin related transmembrane protein 3 [Sourc... [more]
TMX33.014e-3540.36thioredoxin related transmembrane protein 3 [Sourc... [more]
TMX31.508e-2434.94thioredoxin related transmembrane protein 3 [Sourc... [more]
TMX32.462e-1950.00thioredoxin related transmembrane protein 3 [Sourc... [more]
P4HB8.084e-1630.47prolyl 4-hydroxylase subunit beta [Source:HGNC Sym... [more]
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BLAST of SMED30003142 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ZK973.115.633e-3423.93pep chromosome:WBcel235:I:4379325:4381948:-1 gene:... [more]
pdi-21.757e-1728.14Protein disulfide-isomerase 2 [Source:UniProtKB/S... [more]
pdi-21.757e-1728.14Protein disulfide-isomerase 2 [Source:UniProtKB/S... [more]
pdi-12.043e-1733.33Protein disulfide-isomerase 1 [Source:UniProtKB/S... [more]
pdi-12.043e-1733.33Protein disulfide-isomerase 1 [Source:UniProtKB/S... [more]
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BLAST of SMED30003142 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CG50277.887e-3924.36gene:FBgn0036579 transcript:FBtr0075457[more]
Pdi8.972e-1828.57gene:FBgn0286818 transcript:FBtr0346644[more]
Pdi8.972e-1828.57gene:FBgn0286818 transcript:FBtr0075648[more]
ERp602.707e-1430.50gene:FBgn0033663 transcript:FBtr0088058[more]
CG93021.543e-1336.63gene:FBgn0032514 transcript:FBtr0080470[more]
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BLAST of SMED30003142 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tmx3a3.142e-3426.54thioredoxin related transmembrane protein 3a [Sour... [more]
tmx3a3.538e-3427.01thioredoxin related transmembrane protein 3a [Sour... [more]
tmx3a1.141e-2739.71thioredoxin related transmembrane protein 3a [Sour... [more]
tmx3b2.512e-1853.33thioredoxin related transmembrane protein 3b [Sour... [more]
pdia32.250e-1631.58protein disulfide isomerase family A, member 3 [So... [more]
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BLAST of SMED30003142 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nup358.653e-4728.06nucleoporin 35kDa [Source:Xenbase;Acc:XB-GENE-9809... [more]
nup352.185e-4528.37nucleoporin 35kDa [Source:Xenbase;Acc:XB-GENE-9809... [more]
nup352.235e-4327.79nucleoporin 35kDa [Source:Xenbase;Acc:XB-GENE-9809... [more]
nup352.542e-4327.50nucleoporin 35kDa [Source:Xenbase;Acc:XB-GENE-9809... [more]
nup351.202e-3727.21nucleoporin 35kDa [Source:Xenbase;Acc:XB-GENE-9809... [more]
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BLAST of SMED30003142 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Tmx35.233e-4628.94thioredoxin-related transmembrane protein 3 [Sourc... [more]
Tmx33.629e-3536.94thioredoxin-related transmembrane protein 3 [Sourc... [more]
Pdia42.429e-1541.86protein disulfide isomerase associated 4 [Source:M... [more]
Pdia39.141e-1531.13protein disulfide isomerase associated 3 [Source:M... [more]
Pdia61.114e-1335.34protein disulfide isomerase associated 6 [Source:M... [more]
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BLAST of SMED30003142 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q6GNG3|TMX3_XENLA4.949e-4828.78Protein disulfide-isomerase TMX3 OS=Xenopus laevis... [more]
sp|Q5R875|TMX3_PONAB5.832e-4728.71Protein disulfide-isomerase TMX3 OS=Pongo abelii O... [more]
sp|Q96JJ7|TMX3_HUMAN4.474e-4628.24Protein disulfide-isomerase TMX3 OS=Homo sapiens O... [more]
sp|Q8BXZ1|TMX3_MOUSE3.660e-4528.94Protein disulfide-isomerase TMX3 OS=Mus musculus O... [more]
sp|O13704|YEU5_SCHPO4.934e-1832.91Thioredoxin domain-containing protein C13F5.05, mi... [more]
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BLAST of SMED30003142 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210QUZ21.067e-6830.60Protein disulfide-isomerase TMX3 OS=Mizuhopecten y... [more]
A0A267EQ683.126e-6331.34Thioredoxin domain-containing protein (Fragment) O... [more]
A0A2T7NH061.274e-6229.37Thioredoxin domain-containing protein OS=Pomacea c... [more]
V4B8B01.770e-6231.11Thioredoxin domain-containing protein OS=Lottia gi... [more]
A0A0X3PAE86.489e-6230.44Protein disulfide-isomerase TMX3 OS=Schistocephalu... [more]
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BLAST of SMED30003142 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tmx3a2.178e-3427.91protein disulfide-isomerase TMX3-like [Source:NCBI... [more]
tmx3a2.178e-3427.91protein disulfide-isomerase TMX3-like [Source:NCBI... [more]
pdia38.351e-1927.00protein disulfide isomerase family A member 3 [Sou... [more]
pdia41.403e-1437.23protein disulfide isomerase family A member 4 [Sou... [more]
PDIA61.577e-1427.86protein disulfide-isomerase A6 [Source:NCBI gene;A... [more]
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BLAST of SMED30003142 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TMX38.735e-4928.06thioredoxin related transmembrane protein 3 [Sourc... [more]
TMX36.973e-3826.47thioredoxin related transmembrane protein 3 [Sourc... [more]
pdia42.400e-1435.78protein disulfide isomerase family A, member 4 [So... [more]
pdia36.115e-1424.32protein disulfide isomerase family A, member 3 [So... [more]
pdia63.804e-1336.61protein disulfide isomerase family A, member 6 [So... [more]
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BLAST of SMED30003142 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2
Match NameE-valueIdentityDescription
PDI12.872e-1131.87Protein disulfide isomerase; multifunctional oxido... [more]
EPS14.632e-628.00ER protein with chaperone and co-chaperone activit... [more]
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BLAST of SMED30003142 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO415816.308e-4333.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO316342.996e-1534.57Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO413777.568e-1541.98Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO368309.652e-1535.79Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO338432.561e-1440.59Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30003142 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TMX31.605e-5330.14thioredoxin related transmembrane protein 3 [Sourc... [more]
ENSORLT00000009634.26.309e-3626.58protein disulfide-isomerase TMX3-like [Source:NCBI... [more]
pdia33.900e-1524.42protein disulfide isomerase family A member 3 [Sou... [more]
pdia24.043e-1527.94protein disulfide-isomerase [Source:NCBI gene;Acc:... [more]
pdia24.809e-1527.94protein disulfide-isomerase [Source:NCBI gene;Acc:... [more]
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BLAST of SMED30003142 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000029028.10.000e+099.34SMESG000029028.1[more]
SMESG000023147.12.902e-1934.25SMESG000023147.1[more]
SMESG000017020.18.161e-1631.62SMESG000017020.1[more]
SMESG000041085.13.114e-1537.25SMESG000041085.1[more]
SMESG000041085.13.200e-1537.25SMESG000041085.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003142 ID=SMED30003142|Name=SMED30003142|organism=Schmidtea mediterranea sexual|type=transcript|length=1884bp
TAGCAAGACATTATAACTAAATTTCAATAAATTTTCTATGCCCAACACAA
ATACAAATAACAAAATGCTTTACAATTGGTAACAACTAGATTTAAACCAA
TTTCTGTAAAAACAAAAAATAATAGCAATTAAAAGATTAAAAAATAACAG
CAATTATGGTGATTTGATAAATATTGAAATTAAACGACAATTAGAGAAAT
ACATGAATTTCTCAAAACAAAAACAAAAATACCAGTAAACAACAATATAC
ATAATAATAGAACATAATAGTCGTGTAATACAAATTTTTAACTAAGTTTT
TCATTCATTTTTCTTGTCACCATCAGACTTTATATTCAATTCTTCTGATG
ATTTGAGAGCAACTTCATAAAGAGGTATTCTAATCTTCTTTTGCTGACGA
GCTTCAATTCTGCGATTGTCAGCATCACTCGTATCCATGCAACATATTGC
ATAACATAAGAAACTGATAACGCTTAGTGGAACACCGATGATGAACATAG
CTAAAATTGGGGTAGTACTAACAAACTCTATAAGGCCTGTAATAAAATCA
TAACCAGCTCTCTGCATTTTTGCTCTAAATCCAACTCCTCCGTAACCTTC
AATTTCCCCACTTTGAAAAGAATGCAAAAAATCGTGAACAAATTTCTCAT
CAATTGCTTTCATTTTCTGATAATTATTGGAATCAGGGTGAATATAAATG
GTGTGATTCCATGGTTGAAATATTAAAACATTTGGAACAATCAAATTTGT
CATGGCCAAATCATTCATTGATGCAATATCAGTAGACCAAACAAAAAATA
AATCATTTTGTTCATCATTAAAGGATTCAAATGCCAGTTTCTTAGCAAAA
CTAAAAAGTTTCATCGTTGAAGATTCTCGTTCAGGATTATCCGATGGATT
CGAATTTATATCTGATAATAGTGATGAATTGCAAATTAATATTATCAAAG
ATTTCTTCAAGATTTCTAATTCCCATAAATCATGTTGAGAAATTTTTGGA
AATGTGCTAAATCGATTGAATGAAATCCAATTTTGCAATGAGTGATTTAG
TGAACTGATTTCCTCTTTAAAGTGAAAAGAAAGTTTCCTGTCAGGTTTCC
AAACCAAAATTGTACCATTCTCGGTATCAGATAAAGATGAATTCAAATCA
AGATGACTGTAATAACTCTGGGGAATAAATTGATTTTCAATAGAATAAAA
ATAAGAGCGCAATCTTAAAGAATTTGCTAAATCATCATAAACAGCTTTTA
AATAACTAGAACGATCACCGATGTATAGGAAAAAGGTTTCCATTAAATGC
GATTCTATGGCTTCGTGAAATCGGCCTTCACTTGGCAAATGCTTAACTGC
CGGTCCTAAAGCTCGTTTAGAAAACAAAATCAAATGCTCAACAGTTCTAT
CACCATCATGTGTATAAATATCGGTTCCGTTAATATATTTAATTGTTGGA
TAACCGCGAATTTGATAAAATGATGCAACATCAGGATATCGAGTAGCATC
AATACGAGCAATTTTAATGGGTAAATCTTTTATGTGCGCTTCTAATTCAT
TGTAAATTGGTTCCAGTTTCCTACAATAACCACACCACGGTGCGTAAAAT
TTGACCAACCATGGTCCTTTATCTGCTATTTCTATAAAATTTGAATTTAA
TTCAATTACATTACTAAATGCTGGAATTGATATAGATATTATGTATATAA
AAATTTTAAAAAATAATATCATAACTTCACAACTTGTATTTAAAAATATG
TATTTATTAAAATGCGCCAAATTAATAACATTTATTAAGCTAATAAAACA
CTAATAACAAACATTAATTTATTAAAAGTGAATTAGCAAATTTTTAAGCA
TATTCTTTCAATCTTGCACGAGTGCAGGTTTAAA
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protein sequence of SMED30003142-orf-1

>SMED30003142-orf-1 ID=SMED30003142-orf-1|Name=SMED30003142-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=474bp
MILFFKIFIYIISISIPAFSNVIELNSNFIEIADKGPWLVKFYAPWCGYC
RKLEPIYNELEAHIKDLPIKIARIDATRYPDVASFYQIRGYPTIKYINGT
DIYTHDGDRTVEHLILFSKRALGPAVKHLPSEGRFHEAIESHLMETFFLY
IGDRSSYLKAVYDDLANSLRLRSYFYSIENQFIPQSYYSHLDLNSSLSDT
ENGTILVWKPDRKLSFHFKEEISSLNHSLQNWISFNRFSTFPKISQHDLW
ELEILKKSLIILICNSSLLSDINSNPSDNPERESSTMKLFSFAKKLAFES
FNDEQNDLFFVWSTDIASMNDLAMTNLIVPNVLIFQPWNHTIYIHPDSNN
YQKMKAIDEKFVHDFLHSFQSGEIEGYGGVGFRAKMQRAGYDFITGLIEF
VSTTPILAMFIIGVPLSVISFLCYAICCMDTSDADNRRIEARQQKKIRIP
LYEVALKSSEELNIKSDGDKKNE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR036249Thioredoxin-like_sf
IPR013766Thioredoxin_domain
IPR012336Thioredoxin-like_fold
IPR017937Thioredoxin_CS
Vocabulary: biological process
TermDefinition
GO:0045454cell redox homeostasis
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013766Thioredoxin domainPFAMPF00085Thioredoxincoord: 28..115
e-value: 7.4E-21
score: 74.1
IPR013766Thioredoxin domainPROSITEPS51352THIOREDOXIN_2coord: 10..127
score: 13.945
IPR036249Thioredoxin-like superfamilyGENE3DG3DSA:3.40.30.10coord: 16..145
e-value: 1.5E-28
score: 101.6
IPR036249Thioredoxin-like superfamilySUPERFAMILYSSF52833Thioredoxin-likecoord: 23..123
NoneNo IPR availablePANTHERPTHR46426FAMILY NOT NAMEDcoord: 9..446
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..20
score: 0.528
NoneNo IPR availableTMHMMTMhelixcoord: 406..428
IPR017937Thioredoxin, conserved sitePROSITEPS00194THIOREDOXIN_1coord: 39..57