Phospholipase YtpA

Overview
NamePhospholipase YtpA
Smed IDSMED30003096
Length (bp)1085
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Phospholipase YtpA (SMED30003096) t-SNE clustered cells

Violin plots show distribution of expression levels for Phospholipase YtpA (SMED30003096) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Phospholipase YtpA (SMED30003096) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Phospholipase YtpA (SMED30003096) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003096

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30003096SMESG000043160.1 Contig4864GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003096SMESG000038743.1 SMESG000038738.1 Contig4864GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30003096SMESG000038738.1 dd_Smed_v4_6727_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30003096SMESG000043160.1 Contig4864GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30003096SMESG000038743.1 SMESG000038738.1 Contig4864GPL15192PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30003096SMESG000038738.1 dd_Smed_v4_6727_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Category 4 cellSMED30003096SMESG000038738.1 dd_Smed_v4_6727_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Human
Match: MGLL (monoglyceride lipase [Source:HGNC Symbol;Acc:HGNC:17038])

HSP 1 Score: 156.377 bits (394), Expect = 2.266e-44
Identity = 89/281 (31.67%), Postives = 154/281 (54.80%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   +G  KA++F+ HG  EH  S  YE + + L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    LP+FL GHS+GG I++    +RP  F GM L +P ++  P + +   +   K+++++LP + LG ID + +SR+   ++    D L   + +     I++ NAV  ++  +  +T PF++  G AD+L D +G+ LL++ +   DK    +  A H +  E   V++    +I  W+  + +
Sbjct:   21 HLVNADGQYLF-CRYWKPTGTPKALIFVSHGAGEH--SGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSM---QKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTA 295
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Human
Match: MGLL (monoglyceride lipase [Source:HGNC Symbol;Acc:HGNC:17038])

HSP 1 Score: 156.377 bits (394), Expect = 2.266e-44
Identity = 89/281 (31.67%), Postives = 154/281 (54.80%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   +G  KA++F+ HG  EH  S  YE + + L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    LP+FL GHS+GG I++    +RP  F GM L +P ++  P + +   +   K+++++LP + LG ID + +SR+   ++    D L   + +     I++ NAV  ++  +  +T PF++  G AD+L D +G+ LL++ +   DK    +  A H +  E   V++    +I  W+  + +
Sbjct:   21 HLVNADGQYLF-CRYWKPTGTPKALIFVSHGAGEH--SGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSM---QKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTA 295
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Human
Match: MGLL (monoglyceride lipase [Source:HGNC Symbol;Acc:HGNC:17038])

HSP 1 Score: 155.992 bits (393), Expect = 3.235e-44
Identity = 89/281 (31.67%), Postives = 154/281 (54.80%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   +G  KA++F+ HG  EH  S  YE + + L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    LP+FL GHS+GG I++    +RP  F GM L +P ++  P + +   +   K+++++LP + LG ID + +SR+   ++    D L   + +     I++ NAV  ++  +  +T PF++  G AD+L D +G+ LL++ +   DK    +  A H +  E   V++    +I  W+  + +
Sbjct:   31 HLVNADGQYLF-CRYWKPTGTPKALIFVSHGAGEH--SGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSM---QKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTA 305
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Human
Match: MGLL (monoglyceride lipase [Source:HGNC Symbol;Acc:HGNC:17038])

HSP 1 Score: 149.828 bits (377), Expect = 2.417e-42
Identity = 83/247 (33.60%), Postives = 140/247 (56.68%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVF 813
            ++VN DG  LF   Y   +G  KA++F+ HG  EH  S  YE + + L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    LP+FL GHS+GG I++    +RP  F GM L +P ++  P + +   +   K+++++LP + LG ID + +SR+   ++    D L   + +     I++ NAV  ++  +  +T PF++  G AD+L D +G+ LL++ +   DK  
Sbjct:   21 HLVNADGQYLF-CRYWKPTGTPKALIFVSHGAGEH--SGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSM---QKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTL 261
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Human
Match: MGLL (monoglyceride lipase [Source:HGNC Symbol;Acc:HGNC:17038])

HSP 1 Score: 126.331 bits (316), Expect = 2.997e-33
Identity = 83/280 (29.64%), Postives = 136/280 (48.57%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   +G  KA++F+ HG  EH  S  YE + + L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    LP+FL GHS+GG I++    +RP  F GM L +P ++   N  +   +   I              YN              D L   + +     I++ NAV  ++  +  +T PF++  G AD+L D +G+ LL++ +   DK    +  A H +  E   V++    +I  W+  + +
Sbjct:   31 HLVNADGQYLF-CRYWKPTGTPKALIFVSHGAGEH--SGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSM---QKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVL--ANPESATTFKVDI--------------YN-------------SDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTA 275
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Zebrafish
Match: mgll (monoglyceride lipase [Source:ZFIN;Acc:ZDB-GENE-031006-9])

HSP 1 Score: 163.31 bits (412), Expect = 2.871e-47
Identity = 93/276 (33.70%), Postives = 148/276 (53.62%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFL-IFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWL 900
            +IVN DG+ LF   Y    G  KA+V++ HG  EHC    Y  I   L     +V  HDH+GHG S G+R  + NF + VRD L  ID +  +     +L +F+ GHS+GG IS+    +RP  F G+ L  P + +   + +   ++M K+++ L PK+ LG ID   +SRD + +E   KD+L  H  +     ++M +A   ++ ++ ++  PF I HG+ADKL D+ GS+LL  ++  TDK    +  A H +  +        +K++  W+
Sbjct:   21 HIVNADGLHLF-CRYWEPDGPPKALVYVAHGAGEHCGG--YADIAHSLTQHGILVFAHDHVGHGQSEGERMELKNFQIYVRDSLQHIDIMKARY---PKLAVFIVGHSMGGAISILTACERPQDFTGVVLIGPMVQMSAESATPFKVFMAKVLNRLAPKLTLGPIDPKFVSRDPKQVEAYEKDELNYHGGLRVSFGMQMLDATSRIERELPDIRWPFYILHGDADKLCDIRGSRLLYNEAKSTDKKLKVYEEAYHALHHDLPETIESVLKEVSTWI 290
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Zebrafish
Match: mgll (monoglyceride lipase [Source:ZFIN;Acc:ZDB-GENE-031006-9])

HSP 1 Score: 123.25 bits (308), Expect = 2.191e-32
Identity = 72/212 (33.96%), Postives = 114/212 (53.77%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFL-IFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMEN 708
            +IVN DG+ LF   Y    G  KA+V++ HG  EHC    Y  I   L     +V  HDH+GHG S G+R  + NF + VRD L  ID +  +     +L +F+ GHS+GG IS+    +RP  F G+ L  P + +   + +   ++M K+++ L PK+ LG ID   +SRD + +E   KD+L  H  +     ++M +A   ++ ++ +
Sbjct:   64 HIVNADGLHLF-CRYWEPDGPPKALVYVAHGAGEHCGG--YADIAHSLTQHGILVFAHDHVGHGQSEGERMELKNFQIYVRDSLQHIDIMKARY---PKLAVFIVGHSMGGAISILTACERPQDFTGVVLIGPMVQMSAESATPFKVFMAKVLNRLAPKLTLGPIDPKFVSRDPKQVEAYEKDELNYHGGLRVSFGMQMLDATSRIERELPD 269
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Xenopus
Match: slc35d1 (solute carrier family 35 member D1 [Source:Xenbase;Acc:XB-GENE-992383])

HSP 1 Score: 159.458 bits (402), Expect = 2.503e-45
Identity = 88/283 (31.10%), Postives = 159/283 (56.18%), Query Frame = 1 
Query:   67 ESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLI-FPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKI 912
            E  + +N DG  +F   Y   SG+ +A++F+ HG  EHC  C Y+ + + L A++ +V +HDH+GHG S G+R  + +F + VRDV+  +D  + K Q    LP+F+ GHS+GG I++    +RP  F G+ L +P ++  P + ++  ++  K+++ +LP + LGSID N +SR+ + +E    D L  H  +     +++ NA   ++  + +   P ++ HG  DKL D+ GS +++      +K    +  A H +  E   V+S+  ++I  WL+ ++
Sbjct:   40 ELTHYINADGQHIFSR-YWKPSGSPRALMFIVHGAGEHC--CRYDDLAQILTALNFVVFSHDHVGHGQSEGERMTVPDFHIFVRDVIQHLD--LMKKQYPG-LPLFMCGHSMGGAIAILTADERPDDFSGLILISPLVLPNPQSATSFKVFAAKMLNYVLPNLSLGSIDPNFVSRNKKEVEAYTSDPLVYHGGMKVSFGVQLLNATSRVEKALPHFKVPLLLFHGTLDKLCDIRGSHVMMDTIQSEEKTLKVYEGAFHALHKELPEVTSNVFQEIEGWLQQRL 316
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Mouse
Match: Mgll (monoglyceride lipase [Source:MGI Symbol;Acc:MGI:1346042])

HSP 1 Score: 171.4 bits (433), Expect = 2.266e-50
Identity = 95/281 (33.81%), Postives = 157/281 (55.87%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   SG  KA++F+ HG  EHC    Y+ +   L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    +PIFL GHS+GG IS+    +RPT+F GM L +P ++  P + S + +   K+++ +LP M LG ID + +SR+   ++    D L   + +     I++ NAV  ++  M  +T PF++  G AD+L D +G+ LL++ S   DK    +  A H +  E   V++  + ++  W+  +I+
Sbjct:   21 HLVNADGQYLF-CRYWKPSGTPKALIFVSHGAGEHCGR--YDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTI---QKDYPDVPIFLLGHSMGGAISILVAAERPTYFSGMVLISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLSRNKSEVDLYNSDPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIA 295
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Mouse
Match: Mgll (monoglyceride lipase [Source:MGI Symbol;Acc:MGI:1346042])

HSP 1 Score: 171.4 bits (433), Expect = 2.266e-50
Identity = 95/281 (33.81%), Postives = 157/281 (55.87%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   SG  KA++F+ HG  EHC    Y+ +   L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    +PIFL GHS+GG IS+    +RPT+F GM L +P ++  P + S + +   K+++ +LP M LG ID + +SR+   ++    D L   + +     I++ NAV  ++  M  +T PF++  G AD+L D +G+ LL++ S   DK    +  A H +  E   V++  + ++  W+  +I+
Sbjct:   21 HLVNADGQYLF-CRYWKPSGTPKALIFVSHGAGEHCGR--YDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTI---QKDYPDVPIFLLGHSMGGAISILVAAERPTYFSGMVLISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLSRNKSEVDLYNSDPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIA 295
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Mouse
Match: Mgll (monoglyceride lipase [Source:MGI Symbol;Acc:MGI:1346042])

HSP 1 Score: 171.4 bits (433), Expect = 3.530e-50
Identity = 95/281 (33.81%), Postives = 157/281 (55.87%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   SG  KA++F+ HG  EHC    Y+ +   L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    +PIFL GHS+GG IS+    +RPT+F GM L +P ++  P + S + +   K+++ +LP M LG ID + +SR+   ++    D L   + +     I++ NAV  ++  M  +T PF++  G AD+L D +G+ LL++ S   DK    +  A H +  E   V++  + ++  W+  +I+
Sbjct:   37 HLVNADGQYLF-CRYWKPSGTPKALIFVSHGAGEHCGR--YDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTI---QKDYPDVPIFLLGHSMGGAISILVAAERPTYFSGMVLISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLSRNKSEVDLYNSDPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIA 311
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Mouse
Match: Mgll (monoglyceride lipase [Source:MGI Symbol;Acc:MGI:1346042])

HSP 1 Score: 156.377 bits (394), Expect = 3.131e-44
Identity = 95/309 (30.74%), Postives = 158/309 (51.13%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVS----------------------------NVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   SG  KA++F+ HG  EHC    Y+ +   L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    +PIFL GHS+GG IS+    +RPT+F GM L +P ++  P + S                            ++ +   K+++ +LP M LG ID + +SR+   ++    D L   + +     I++ NAV  ++  M  +T PF++  G AD+L D +G+ LL++ S   DK    +  A H +  E   V++  + ++  W+  +I+
Sbjct:   21 HLVNADGQYLF-CRYWKPSGTPKALIFVSHGAGEHCGR--YDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTI---QKDYPDVPIFLLGHSMGGAISILVAAERPTYFSGMVLISPLVLANPESASTLKDKLHITKTSLEKAAEVSVTWIPYAFHLWVLAAKLLNFVLPNMTLGRIDSSVLSRNKSEVDLYNSDPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIA 323
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Mouse
Match: Mgll (monoglyceride lipase [Source:MGI Symbol;Acc:MGI:1346042])

HSP 1 Score: 137.887 bits (346), Expect = 5.361e-38
Identity = 85/281 (30.25%), Postives = 136/281 (48.40%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   SG  KA++F+ HG  EHC    Y+ +   L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    +PIFL GHS+GG IS+    +RPT+F GM L +P ++  P + S + +   K+++ +LP M LG ID + +SR+                                                G AD+L D +G+ LL++ S   DK    +  A H +  E   V++  + ++  W+  +I+
Sbjct:   21 HLVNADGQYLF-CRYWKPSGTPKALIFVSHGAGEHCGR--YDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTI---QKDYPDVPIFLLGHSMGGAISILVAAERPTYFSGMVLISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLSRNKS---------------------------------------------EGSADRLCDSKGAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIA 250
[Close]
BLAST of Phospholipase YtpA vs. UniProt/SwissProt
Match: sp|Q8R431|MGLL_RAT (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 176.407 bits (446), Expect = 1.827e-51
Identity = 96/282 (34.04%), Postives = 162/282 (57.45%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKISI 918
            ++VN DG  LF   Y   SG  KA++F+ HG  EHC    Y+ + + L  +D +V  HDH+GHG S G+R ++ +F + VRD+L  ++ V    +   ++P+FL GHS+GG IS+ A  +RPT F GM L +P ++  P + S + +   K+++ +LP + LG ID + +SR+   ++    D L  H+ +     I++ NAV  ++  M  +T PF++  G AD+L D +G+ LL++ SP  DK    +  A H +  E   V++  + +I  W+  +I++
Sbjct:   21 HLVNADGQYLF-CRYWKPSGTPKALIFVSHGAGEHCGR--YDELAQMLKRLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDLLQHVNTV---QKDYPEVPVFLLGHSMGGAISILAAAERPTHFSGMILISPLILANPESASTLKVLAAKLLNFVLPNISLGRIDSSVLSRNKSEVDLYNSDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSPSQDKTLKMYEGAYHVLHKELPEVTNSVLHEINTWVSHRIAV 296
[Close]
BLAST of Phospholipase YtpA vs. UniProt/SwissProt
Match: sp|O35678|MGLL_MOUSE (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.585e-49
Identity = 95/281 (33.81%), Postives = 157/281 (55.87%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   SG  KA++F+ HG  EHC    Y+ +   L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    +PIFL GHS+GG IS+    +RPT+F GM L +P ++  P + S + +   K+++ +LP M LG ID + +SR+   ++    D L   + +     I++ NAV  ++  M  +T PF++  G AD+L D +G+ LL++ S   DK    +  A H +  E   V++  + ++  W+  +I+
Sbjct:   21 HLVNADGQYLF-CRYWKPSGTPKALIFVSHGAGEHCGR--YDELAHMLKGLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTI---QKDYPDVPIFLLGHSMGGAISILVAAERPTYFSGMVLISPLVLANPESASTLKVLAAKLLNFVLPNMTLGRIDSSVLSRNKSEVDLYNSDPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIA 295
[Close]
BLAST of Phospholipase YtpA vs. UniProt/SwissProt
Match: sp|Q99685|MGLL_HUMAN (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 156.377 bits (394), Expect = 1.088e-43
Identity = 89/281 (31.67%), Postives = 154/281 (54.80%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            ++VN DG  LF   Y   +G  KA++F+ HG  EH  S  YE + + L  +D +V  HDH+GHG S G+R ++ +F + VRDVL  +D +    +    LP+FL GHS+GG I++    +RP  F GM L +P ++  P + +   +   K+++++LP + LG ID + +SR+   ++    D L   + +     I++ NAV  ++  +  +T PF++  G AD+L D +G+ LL++ +   DK    +  A H +  E   V++    +I  W+  + +
Sbjct:   21 HLVNADGQYLF-CRYWKPTGTPKALIFVSHGAGEH--SGRYEELARMLMGLDLLVFAHDHVGHGQSEGERMVVSDFHVFVRDVLQHVDSM---QKDYPGLPVFLLGHSMGGAIAILTAAERPGHFAGMVLISPLVLANPESATTFKVLAAKVLNLVLPNLSLGPIDSSVLSRNKTEVDIYNSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTA 295
[Close]
BLAST of Phospholipase YtpA vs. UniProt/SwissProt
Match: sp|O07427|MGLL_MYCTU (Monoacylglycerol lipase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv0183 PE=1 SV=2)

HSP 1 Score: 120.553 bits (301), Expect = 1.882e-30
Identity = 84/278 (30.22%), Postives = 130/278 (46.76%), Query Frame = 1 
Query:   85 NVDGIKLFRMIYH--STSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDV---LYDIDDVVN-KMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWL 900
            N  GI   R++Y   +   A +A+V L HGL EH +   Y+ + + L A   +    DH GHG S G R       +LVRD+     D D +V    +        + GHS+GG I     ++RP  +  M L AP +     VS V     K++ V++P +P+  +D+  ISRD E ++    D L  H R+PA     +    E +  +   +T P ++ HG  D+L  +EGS+ LV+     D     +P   HE+  EP    +  +  +V WL
Sbjct:    9 NFAGIGDVRIVYDVWTPDTAPQAVVVLAHGLGEHARR--YDHVAQRLGAAGLVTYALDHRGHGRSGGKR-------VLVRDISEYTADFDTLVGIATREYPGCKRIVLGHSMGGGIVFAYGVERPDNYDLMVLSAPAVAAQDLVSPVVAVAAKLLGVVVPGLPVQELDFTAISRDPEVVQAYNTDPLVHHGRVPAGIGRALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVECVGSADVQLKEYPGLYHEVFNEPER--NQVLDDVVAWL 275
[Close]
BLAST of Phospholipase YtpA vs. UniProt/SwissProt
Match: sp|A0QNZ7|MGLL_MYCS2 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 2.647e-26
Identity = 71/256 (27.73%), Postives = 117/256 (45.70%), Query Frame = 1 
Query:   94 GIKLFRMIYHSTSGAT--KAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEP 855
            G+   R++Y   +  T  + +V L HG  EH     Y  + +   A   +V   DH GHG S G R  + +    V D    +    N   T   LP  + GHS+GG I      + P  +  M L  P +     VS V + + K++  L P +P+ ++D + +SRD E +   + D +  H ++PA     +    +++  +   +T P ++ HG+ D+L  V GS+LLV +    D     +P   HE+  EP
Sbjct:   13 GVGGVRIVYDVWTPDTDPRGVVVLAHGYAEHAGR--YHHVAQRFGAAGLLVYALDHRGHGRSGGKRVHLRDLSEFVEDFRTLVGIAANDHPT---LPRIVLGHSMGGGIVFAYGARYPGEYSAMVLSGPAVNAHDGVSPVLVAVAKVLGKLAPGIPVENLDADAVSRDPEVVAAYKADPMVHHGKLPAGIARALIGLGQSMPQRAAALTAPLLVVHGDKDRLIPVAGSRLLVDRVASEDVHLKVYPGLYHEVFNEP 263
[Close]
BLAST of Phospholipase YtpA vs. TrEMBL
Match: A0A224Z3S0 (Lysophospholipase OS=Rhipicephalus zambeziensis OX=60191 PE=4 SV=1)

HSP 1 Score: 189.504 bits (480), Expect = 6.909e-54
Identity = 95/283 (33.57%), Postives = 162/283 (57.24%), Query Frame = 1 
Query:   67 ESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFL-IFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKI 912
            +S    N DG K+  + + S     +A+VFL HG  EHC    Y+ + + L  + C V +HDH+GHG S G R ++ + D+ V D+L  +D V  K  ++   P+FL+GHS+GGL+   A  +RP    G+ + AP L +     + + + + +I+  ++P +P+G++D + +S+D E ++ + KD LR H R+       M NA+E+L+ +++++ TPF I HG ADKL D+ GS+   KK+P  DK F  + +  H +  EP +++   ++ I  W   +I
Sbjct:   25 DSGSFRNRDGHKISCLTW-SPEVEPRALVFLAHGYAEHCHGPCYDMLARKLVEMGCYVFSHDHVGHGKSEGPRALVKSADIYVDDILTHVDLVKQKYPSR---PVFLFGHSMGGLLVALAAERRPQDIAGIVMMAPMLAVDKEQATWLKMTLARILGRIVPTLPIGNLDLSLVSKDPEIVDRMTKDPLRYHGRVRVGWAAAMLNALEDLQAKVDSIDTPFFIQHGSADKLCDIGGSEDFYKKAPTKDKSFKVYKDCYHSLLTEPEDMAQQVLQDIANWYSARI 303
[Close]
BLAST of Phospholipase YtpA vs. TrEMBL
Match: A0A1E1X759 (Putative lysophospholipase (Fragment) OS=Amblyomma aureolatum OX=187763 PE=2 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 4.804e-53
Identity = 97/278 (34.89%), Postives = 153/278 (55.04%), Query Frame = 1 
Query:   67 ESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFL-IFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEW 897
            +S    N DG K+    + S     +A+VFL HG  EHC    Y+S+ + L  + C V  HDH+GHG S G RG + + D+ V D+L  +D V  K   +   P+FL+GHS+GG + V + L+RP  + GM L  P + I P   +   + + +I+  ++P +P+G +D + +S+D + +  +  D LR H  +       M NA+E+L+ ++E V  PF+I HG ADKL D+ GS+   KK+P  DK    + +  H +  EPG +    +K I +W
Sbjct:    8 DSGSFRNRDGYKIACTTW-SPEAQPRALVFLAHGYAEHCHVPCYDSLARTLVELGCYVFAHDHVGHGKSEGPRGTVKSADIYVDDILTHVDLVRQKFPGR---PVFLFGHSMGGTMCVRSALERPGEYAGMVLNGPAVRIDPVQGTWTKVTLARILGRVVPNLPIGDLDLSLVSKDPDTVAWMASDPLRYHGSVRMGWAAAMLNALEDLQAKIELVDIPFLIQHGSADKLCDLAGSEDFYKKAPSKDKSMKVYEDCYHSLLTEPGEMGQQVLKDITDW 281
[Close]
BLAST of Phospholipase YtpA vs. TrEMBL
Match: A0A023FL54 (Putative lysophospholipase OS=Amblyomma cajennense OX=34607 PE=2 SV=1)

HSP 1 Score: 186.808 bits (473), Expect = 6.968e-53
Identity = 98/278 (35.25%), Postives = 152/278 (54.68%), Query Frame = 1 
Query:   67 ESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFL-IFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEW 897
            +S    N DG K+    + S     +A+VFL HG  EHC    Y+S+ + L  + C V  HDH+GHG S G RG + + D+ V D+L  +D V  K   +   P++L+GHS+GG + V + L+RP  F GM L  P + I P   +   + + +I+  ++P +P+G +D + +SRD + +  +  D LR H  +       M NA+E+L+ ++E V  PF+I HG ADKL D+ GS+   KK+P  DK    +    H +  EPG +    +K I +W
Sbjct:   25 DSGSFRNRDGYKIACTTW-SPEVQPRALVFLAHGYAEHCHVPCYDSLARTLVELGCYVFAHDHVGHGKSEGPRGTVKSADIYVDDILTHVDLVRQKFPGR---PVYLFGHSMGGTMCVRSALERPGEFAGMVLNGPAVRIDPVQGTWTKMTLARILGRVVPNLPIGDLDLSLVSRDPDTVAWMANDPLRYHGSVRMGWAAAMLNALEDLQAKIELVDIPFLIQHGSADKLCDLAGSEEFYKKAPSKDKSMKVYKECYHSLLTEPGEMGQQVLKDIADW 298
[Close]
BLAST of Phospholipase YtpA vs. TrEMBL
Match: A0A1E1X7Q5 (Putative lysophospholipase (Fragment) OS=Amblyomma aureolatum OX=187763 PE=2 SV=1)

HSP 1 Score: 183.726 bits (465), Expect = 7.056e-52
Identity = 97/278 (34.89%), Postives = 152/278 (54.68%), Query Frame = 1 
Query:   67 ESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFL-IFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEW 897
            +S    N DG K+    + S     +A+VFL HG  EHC    Y+S+ + L  + C V  HDH+GHG S G RG + + D+ V D+L  +D V  K   +   P+FL+GHS+GGL+   A  +RP    G+ + AP L I     S + + + +I+  ++P +P+G +D + +S+D + +  +  D LR H  +       M NA+E+L+ ++E V  PF+I HG ADKL D+ GS+   KK+P  DK    + +  H +  EPG +    +K I +W
Sbjct:    8 DSGSFRNRDGYKIACTTW-SPEAQPRALVFLAHGYAEHCHVPCYDSLARTLVELGCYVFAHDHVGHGKSEGPRGTVKSADIYVDDILTHVDLVRQKFPGR---PVFLFGHSMGGLLVAMAAERRPKDIAGLIMMAPLLAIAKEQGSWLKVTLARILGRVVPNLPIGDLDLSLVSKDPDTVAWMASDPLRYHGSVRMGWAAAMLNALEDLQAKIELVDIPFLIQHGSADKLCDLAGSEDFYKKAPSKDKSMKVYEDCYHSLLTEPGEMGQQVLKDITDW 281
[Close]
BLAST of Phospholipase YtpA vs. TrEMBL
Match: A7T3M9 (Predicted protein OS=Nematostella vectensis OX=45351 GN=v1g195674 PE=4 SV=1)

HSP 1 Score: 180.644 bits (457), Expect = 1.472e-50
Identity = 99/278 (35.61%), Postives = 162/278 (58.27%), Query Frame = 1 
Query:   85 NVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVAL-YMGKIVSVLLPKMPL-GSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKI 912
            N DG+ +    + S S   KA++F+CHG  +H +  Y + + + L      V++HDH+GHG S G+R  I +    VRD+   ID ++ K +    LPI+L+GHS+GGLI+V A  +RPTFFKG+ L AP LI   +  N  + ++GK+VS + P + L  ++D N +SRD E ++   +D L  H  +     + + +AV+ ++  ME++  PF++ HG AD L  +EGSK L +++   DK    +    H++  EP + S+  +K I+EWL  ++
Sbjct:   25 NKDGLSISTRTWTSQSEQPKALIFICHGYGDHSKR-YSKFLAQALVDEGFFVLSHDHVGHGKSEGERAQIDSLQKYVRDIFDHIDQIIPKYEG---LPIYLFGHSMGGLIAVLAAQRRPTFFKGVVLSAPALIVDPHKDNKCMRFLGKMVSWVAPSLQLLPAMDPNSMSRDPEQVKAYAEDPLVWHGGVKVGIGLAIAHAVDEVQASMESIKWPFLVLHGTADTLCLMEGSKQLERRAGSKDKTIKTYDGYYHDLLKEPKDDSTVILKDIIEWLNARM 298
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Cavefish
Match: mgll (monoglyceride lipase [Source:NCBI gene;Acc:103026761])

HSP 1 Score: 164.081 bits (414), Expect = 1.220e-47
Identity = 89/276 (32.25%), Postives = 149/276 (53.99%), Query Frame = 1 
Query:   76 YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFL-IFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWL 900
            +IV+ DG+ LF   Y       +A+VF+ HG  EHC    Y  +   L      V  HDH+GHG S G+R  I NF + +RD L  ID +  +  +   LP+F+ GHS+GG IS+ A  +RP  F G+ L  P + + P + +   +++ K++S ++P + LG+ID   +SRD + +E    D+L  H  +     +++  A   ++ ++ N+T PF I HG+ADKL D+ GS L+  ++  TDK    +  A H +  +    +   ++++  W+
Sbjct:   23 HIVSADGLHLF-CRYWEPEAPPRALVFIAHGAGEHC--GMYTDVAHTLVQKGLYVFAHDHVGHGQSEGERMNIKNFQIYIRDALQHIDLIRARHPS---LPVFILGHSMGGAISILAACERPQDFVGVVLIGPLIQMNPESATPFKVFLAKVLSHMIPSLTLGAIDPKSVSRDPKQVEAYEADELNYHGGMRVSFGMQLMTAANRIERELPNITWPFYILHGDADKLCDIGGSHLMFNQAKSTDKKLKVYEGAYHALHHDLPETAESVLQEVSSWI 292
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Sea Lamprey
Match: mgll (monoglyceride lipase [Source:ZFIN;Acc:ZDB-GENE-031006-9])

HSP 1 Score: 132.109 bits (331), Expect = 1.124e-36
Identity = 76/258 (29.46%), Postives = 128/258 (49.61%), Query Frame = 1 
Query:  142 KAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKI 912
            +A+VF+ HGL EHC    Y+ + + L     +V  HDH+GHG S G+R       + VRD L        +     +LP FL GHS+GG ++V    + P  F G+ L +P +   P+  S   +YM +I++ + P+ P+GS++   +SRD   ++    D L  H R+     + +      ++  + +   PF++  G AD L D EG++L    +P  DK    + +A H +  E   V    +K+I  W+  ++
Sbjct:    1 RALVFVSHGLGEHCGR--YDEVARMLVQHGVLVFAHDHVGHGRSEGERVSEARLGVYVRDCLQHARLARTR---HPELPCFLLGHSMGGAVAVAVASECPDDFAGVILISPLVTLSPSVTSPTLMYMARIMNYMWPRCPVGSVNTAWLSRDSNEVQAYEYDPLVHHGRLSVAMGLGLSETSARVRWLVPSARWPFLLLQGGADLLCDPEGARLFYDLAPSQDKTLRWYQDAYHVLHKELPEVKEAVLKEIESWVLARL 253
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Sea Lamprey
Match: mgll (monoglyceride lipase [Source:ZFIN;Acc:ZDB-GENE-031006-9])

HSP 1 Score: 130.568 bits (327), Expect = 9.987e-36
Identity = 79/258 (30.62%), Postives = 128/258 (49.61%), Query Frame = 1 
Query:  130 SGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIF-PTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWL 900
             G    +V + HG  EHC+   Y+ +   L+A+      HDH+GHG S G R  + +F + VRD L   D +  +      LPIFL GHS+GG IS+ A  +R     G+ L  P +   P  VS   +++ KI   LLP + L SI    ++R+   +E    D L  H+ +    +  + +AV  ++  + +VT PF++ HG+ D+L  V GSK   +++   DK    +  A H +  E    +   +++I  W+
Sbjct:   37 GGGKAGLVHVIHGAGEHCRR--YDEVASRLSALGYFAFAHDHVGHGQSEGPRMQVSDFQVFVRDTLQHFDLICKRY---PDLPIFLLGHSMGGAISILAASERSDKVAGVVLIGPLIEGNPDIVSPFKIFLMKIFKYLLPSLTLASIGPTLVTRNKAEVELFESDPLVHHAGLSVSFLSTLMDAVARIEALLPDVTWPFLLLHGDEDQLCFVGGSKAFHERARSADKTLHIYEGAYHALHKEIPECAGTVLEEIEAWI 289
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Sea Lamprey
Match: mgll (monoglyceride lipase [Source:ZFIN;Acc:ZDB-GENE-031006-9])

HSP 1 Score: 95.5153 bits (236), Expect = 4.877e-23
Identity = 76/265 (28.68%), Postives = 126/265 (47.55%), Query Frame = 1 
Query:  142 KAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKR--PTFFKGMCLEAPFLIFPTNVSNVALYM-GKIVSVLLPKMPLGSIDYNDI---SRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVK--KSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKI 912
            + +V + HG  EHC    Y+ + K L     +V  HDH+GHG S   R    + D+ VRD L  +  V     T  +LP FL GHSLG + S  A  +R  P   + + L +P      + +   L   G+++ + LP   LGS+D + +   SR+   +     D L  H R+     + + + V  L   M +V+ PF++ HG  D+L D  GS++LV+  +    D     +  A H +  E  +V    +++I +W+  ++
Sbjct:    1 RGLVCVMHGAGEHCGR--YDELAKTLTRHSLLVFGHDHVGHGRSTRVRLADIHVDMHVRDCLQHVRRVRG---THPRLPCFLLGHSLGHVDSKCAEGERGEPEDPETIHLFSPLSRRSISSAKSGLRAHGEVLQLHLPPFTLGSLDPSLLWPHSREGSEVRDYASDPLVYHGRVRVAFAVRLLHTVSLLARLMPSVSWPFLLLHGSRDQLCDPAGSQMLVRPGRQHGQDHQGEMYEGAYHVLHRELPDVKEAVLREIQQWIMARL 260
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Yeast
Match: YJU3 (Monoglyceride lipase (MGL); functional ortholog of mammalian MGL, localizes to lipid particles and membranes, also member of the eukaryotic serine hydrolase family [Source:SGD;Acc:S000001577])

HSP 1 Score: 63.1586 bits (152), Expect = 9.348e-12
Identity = 66/289 (22.84%), Postives = 126/289 (43.60%), Query Frame = 1 
Query:   85 NVDGIKLFRMIYHSTSGAT--KAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDR--GMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISV-YATL-KRPTFFKGMCLEAPFLIF-PTNVSNVALYM-GKIVSVLLPKMPLGS-IDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTT-------PFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEI-RMEPGNVSSDFVKQIVEWL 900
            N DG K   M +   +G    +  V L HG  E+ +  +   ++ HL+       T D  G G+++  R  G+   + +   D+ + ++  +++ + K  +P+F++GHS+GG I + YA   K      G     P +I  P  + N    +   +++  LP++ + + +D   I+ D  Y   +  D + +      + + +       L     N          P II HG+ D ++D +GS+  ++  P  DK    +P A+H I  +E   V +     + +WL
Sbjct:   19 NFDGAKFGYMFWPVQNGTNEVRGRVLLIHGFGEYTKIQF--RLMDHLSLNGYESFTFDQRGAGVTSPGRSKGVTDEYHVF-NDLEHFVEKNLSECKAKG-IPLFMWGHSMGGGICLNYACQGKHKNEISGYIGSGPLIILHPHTMYNKPTQIIAPLLAKFLPRVRIDTGLDLKGITSDKAYRAFLGSDPMSVPLYGSFRQIHDFMQRGAKLYKNENNYIQKNFAKDKPVIIMHGQDDTINDPKGSEKFIQDCPSADKELKLYPGARHSIFSLETDKVFNTVFNDMKQWL 303
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Nematostella
Match: EDO29435 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T3M9])

HSP 1 Score: 180.644 bits (457), Expect = 2.035e-54
Identity = 99/278 (35.61%), Postives = 162/278 (58.27%), Query Frame = 1 
Query:   85 NVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVAL-YMGKIVSVLLPKMPL-GSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKI 912
            N DG+ +    + S S   KA++F+CHG  +H +  Y + + + L      V++HDH+GHG S G+R  I +    VRD+   ID ++ K +    LPI+L+GHS+GGLI+V A  +RPTFFKG+ L AP LI   +  N  + ++GK+VS + P + L  ++D N +SRD E ++   +D L  H  +     + + +AV+ ++  ME++  PF++ HG AD L  +EGSK L +++   DK    +    H++  EP + S+  +K I+EWL  ++
Sbjct:   25 NKDGLSISTRTWTSQSEQPKALIFICHGYGDHSKR-YSKFLAQALVDEGFFVLSHDHVGHGKSEGERAQIDSLQKYVRDIFDHIDQIIPKYEG---LPIYLFGHSMGGLIAVLAAQRRPTFFKGVVLSAPALIVDPHKDNKCMRFLGKMVSWVAPSLQLLPAMDPNSMSRDPEQVKAYAEDPLVWHGGVKVGIGLAIAHAVDEVQASMESIKWPFLVLHGTADTLCLMEGSKQLERRAGSKDKTIKTYDGYYHDLLKEPKDDSTVILKDIIEWLNARM 298
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Medaka
Match: mgll (monoglyceride lipase [Source:NCBI gene;Acc:101169855])

HSP 1 Score: 167.162 bits (422), Expect = 1.130e-48
Identity = 91/283 (32.16%), Postives = 159/283 (56.18%), Query Frame = 1 
Query:   67 ESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFL-IFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKI 912
            E  +IVN DG+ LF   Y   +   +A+VF+ HG  EH  S  Y+ I + L  +  +V  HDH+GHG S G+R  I +F + VRD L  ID + ++      LP+F+ GHS+GG IS+    +RP+   G+ L  P + + P + +   +++ K+++ ++P + LGSI+   +SRD   +E    D+L  H R+     I++  A E ++ ++ +++ PF+I HG+ DKL D+ GSK++ +K+  +DK    +  A H +  +   V+   +K +  W+  ++
Sbjct:   27 ELPHIVNADGLHLF-CRYWEPAAPPRALVFVVHGAGEH--SGPYDEIAQRLKELSLLVFAHDHVGHGQSEGERMNIKDFQIYVRDSLQHIDLMKSR---HPDLPVFIVGHSMGGAISILTACERPSEISGVVLIGPMVQMNPESATPFKVFVAKLLNHMMPSLTLGSIESRWVSRDKTQVEAYDNDELNYHGRLRVSFGIQLMGAAERIEREIPSISWPFLILHGDDDKLCDIRGSKMMHEKAASSDKKLKIYEGAYHALHHDLPEVAESVLKDVTSWITERL 303
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Medaka
Match: mgll (monoglyceride lipase [Source:NCBI gene;Acc:101169855])

HSP 1 Score: 167.162 bits (422), Expect = 1.274e-48
Identity = 91/283 (32.16%), Postives = 159/283 (56.18%), Query Frame = 1 
Query:   67 ESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFL-IFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKI 912
            E  +IVN DG+ LF   Y   +   +A+VF+ HG  EH  S  Y+ I + L  +  +V  HDH+GHG S G+R  I +F + VRD L  ID + ++      LP+F+ GHS+GG IS+    +RP+   G+ L  P + + P + +   +++ K+++ ++P + LGSI+   +SRD   +E    D+L  H R+     I++  A E ++ ++ +++ PF+I HG+ DKL D+ GSK++ +K+  +DK    +  A H +  +   V+   +K +  W+  ++
Sbjct:   30 ELPHIVNADGLHLF-CRYWEPAAPPRALVFVVHGAGEH--SGPYDEIAQRLKELSLLVFAHDHVGHGQSEGERMNIKDFQIYVRDSLQHIDLMKSR---HPDLPVFIVGHSMGGAISILTACERPSEISGVVLIGPMVQMNPESATPFKVFVAKLLNHMMPSLTLGSIESRWVSRDKTQVEAYDNDELNYHGRLRVSFGIQLMGAAERIEREIPSISWPFLILHGDDDKLCDIRGSKMMHEKAASSDKKLKIYEGAYHALHHDLPEVAESVLKDVTSWITERL 306
[Close]
BLAST of Phospholipase YtpA vs. Ensembl Medaka
Match: mgll (monoglyceride lipase [Source:NCBI gene;Acc:101169855])

HSP 1 Score: 167.162 bits (422), Expect = 2.329e-48
Identity = 91/283 (32.16%), Postives = 159/283 (56.18%), Query Frame = 1 
Query:   67 ESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFL-IFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKI 912
            E  +IVN DG+ LF   Y   +   +A+VF+ HG  EH  S  Y+ I + L  +  +V  HDH+GHG S G+R  I +F + VRD L  ID + ++      LP+F+ GHS+GG IS+    +RP+   G+ L  P + + P + +   +++ K+++ ++P + LGSI+   +SRD   +E    D+L  H R+     I++  A E ++ ++ +++ PF+I HG+ DKL D+ GSK++ +K+  +DK    +  A H +  +   V+   +K +  W+  ++
Sbjct:   57 ELPHIVNADGLHLF-CRYWEPAAPPRALVFVVHGAGEH--SGPYDEIAQRLKELSLLVFAHDHVGHGQSEGERMNIKDFQIYVRDSLQHIDLMKSR---HPDLPVFIVGHSMGGAISILTACERPSEISGVVLIGPMVQMNPESATPFKVFVAKLLNHMMPSLTLGSIESRWVSRDKTQVEAYDNDELNYHGRLRVSFGIQLMGAAERIEREIPSISWPFLILHGDDDKLCDIRGSKMMHEKAASSDKKLKIYEGAYHALHHDLPEVAESVLKDVTSWITERL 333
[Close]
BLAST of Phospholipase YtpA vs. Planmine SMEST
Match: SMESG000038738.1 (SMESG000038738.1)

HSP 1 Score: 593.964 bits (1530), Expect = 0.000e+0
Identity = 288/290 (99.31%), Postives = 289/290 (99.66%), Query Frame = 1 
Query:   49 MSEEKIESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKISI 918
            MSEEKIESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLS+VEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLR KISI
Sbjct:    1 MSEEKIESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSEVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRFKISI 290
[Close]
BLAST of Phospholipase YtpA vs. Planmine SMEST
Match: SMESG000038743.1 (SMESG000038743.1)

HSP 1 Score: 557.37 bits (1435), Expect = 0.000e+0
Identity = 266/289 (92.04%), Postives = 279/289 (96.54%), Query Frame = 1 
Query:   49 MSEEKIESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKIS 915
            MSEEK E+DYI+NVDGIKLFRMIYHSTSG TKAIVFLCHGLFEHCQ+CYYE IIKHLN IDC+VVTHDHMGHGLSNGDRGMIY+FDLLVRDVL DIDDV+NKMQTKSQLPIFLYGHSLGGL+SVYATLKRPTFFKGMCLEAPFL+FPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHS IPAKT+IE++NAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDK+FIPFP AKH+IRMEPGNVSSDFVKQIVEW R KI 
Sbjct:    1 MSEEKSENDYIINVDGIKLFRMIYHSTSGTTKAIVFLCHGLFEHCQACYYEPIIKHLNDIDCLVVTHDHMGHGLSNGDRGMIYDFDLLVRDVLSDIDDVINKMQTKSQLPIFLYGHSLGGLVSVYATLKRPTFFKGMCLEAPFLMFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSGIPAKTIIEIYNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKIFIPFPGAKHQIRMEPGNVSSDFVKQIVEWFRNKIG 289
[Close]
BLAST of Phospholipase YtpA vs. Planmine SMEST
Match: SMESG000038748.1 (SMESG000038748.1)

HSP 1 Score: 313.538 bits (802), Expect = 3.875e-106
Identity = 141/284 (49.65%), Postives = 204/284 (71.83%), Query Frame = 1 
Query:   49 MSEEKIESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWL 900
            M+ +  + D+ VN DGIKLFR++Y  T    +A+VF+CHGLFEHCQ+ Y++ +I+ L    CMV+THDH GHGLS GDRGMI +FD LVRDVL DID+ + +     Q+P+FLYG+SLGGLI +YA+L+R  FFKG+CLEAP L+FP+    +    GK++S   P      +DY+DI R+ +Y+  +  D LRIHS +PAKT +E++ A + LKG ++ ++TPFI+ HG+AD++S++ GS+ L++ +P+ DK FI F  AKH++RME  NV+ +F K I+EW+
Sbjct:    1 MAGKNRDKDFFVNPDGIKLFRLVYPPTVRPYRAVVFICHGLFEHCQASYFKPVIRPLVNSGCMVITHDHRGHGLSEGDRGMINSFDSLVRDVLLDIDETLVRENIPQQVPLFLYGYSLGGLICIYASLRRDRFFKGLCLEAPLLVFPSKFGIITPSFGKLISRYFPTFEFNKLDYDDICRNKDYVHTLYGDHLRIHSGVPAKTAMEIYRAADYLKGCLKEISTPFIVCHGDADEISEIRGSEYLMEIAPVQDKTFIIFQRAKHQLRMEIENVNIEFSKNILEWI 284
[Close]
BLAST of Phospholipase YtpA vs. Planmine SMEST
Match: SMESG000038746.1 (SMESG000038746.1)

HSP 1 Score: 293.123 bits (749), Expect = 4.511e-98
Identity = 139/284 (48.94%), Postives = 192/284 (67.61%), Query Frame = 1 
Query:   49 MSEEKIESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWL 900
            M  E  E+DY +N D IKLFR++Y       KA+VF+CHGLFEHCQS Y + II  L  I CMVVTHDH GHGLS G RGMIY+   LVRDVL DID+V+ +      +P+F+YGHS+GGLISV+A+L+R +FF GMCLEAP L  P  V    +++G+I++ +LP + +   + + I R++E L+ +  DKL IH  +PA++ + +  A++ LK  ++NV TPFIIGHG+ADKL ++ GS   V+    TDK F+ FP AKH +R E  NV  +F  +I+ W+
Sbjct:    1 MDGENTENDYFLNPDKIKLFRLVYKPIDSNVKALVFICHGLFEHCQSGYLKLIINPLKDIGCMVVTHDHRGHGLSEGKRGMIYDIHSLVRDVLLDIDEVMIQQNLNKNIPVFIYGHSMGGLISVFASLERKSFFNGMCLEAPALSIPKIVGKGTIFLGRIMNKVLPSISIAKSNKSYICRNLECLKEIENDKLVIHRGLPARSGMSIMKALQQLKPLIKNVDTPFIIGHGDADKLCEIAGSHYFVENCSATDKSFLTFPGAKHRLRAEIDNVDVEFSTKIINWI 284
[Close]
BLAST of Phospholipase YtpA vs. Planmine SMEST
Match: SMESG000004815.1 (SMESG000004815.1)

HSP 1 Score: 202.216 bits (513), Expect = 1.675e-62
Identity = 98/290 (33.79%), Postives = 169/290 (58.28%), Query Frame = 1 
Query:   52 SEEKIESD-YIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYYESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVVNKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNVSNVALYMGKI--VSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIPAKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPITDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKI 912
            S + IE++ Y +N DG+KLF  I+   SG  +A +F+ HGL EH    Y+  I+K L  I C+V +HDH+GHG S+G+R ++   D L  DV+  I+D+ ++ +    +PIF YGHSLGGLI+   +L +P   KGM LEAP L         + YM  +  +S L+P   +    + +++R+ E +++   D  R    +   T + +  A++ ++ +++++ TPF++ HG+ D +    GSK+L   + + DK++I +  A H +R++ G +  DF  ++VEW+  +I
Sbjct:    4 SSDVIENENYFINKDGLKLFEQIWFPESGEIRAFIFIIHGLSEHSSFYYFNPIVKPLLDIGCLVASHDHVGHGKSDGNREVVEKIDKLTNDVILHIEDIKSRFKLSDNIPIFAYGHSLGGLIACLLSLDKPQILKGMVLEAPALTLNGRDDTGSWYMFIVYMLSFLMPSFVIKKPTFENLTRNNEVIQNALTDTFRNKGGLTMGTAVNIGYAIQGVQSRLKDIKTPFLVAHGKKDVVVSSTGSKILCDVAEVEDKLYIEYEEAYHLLRVDSGEICEDFTCKLVEWINQRI 293
The following BLAST results are available for this feature:
BLAST of Phospholipase YtpA vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MGLL2.266e-4431.67monoglyceride lipase [Source:HGNC Symbol;Acc:HGNC:... [more]
MGLL2.266e-4431.67monoglyceride lipase [Source:HGNC Symbol;Acc:HGNC:... [more]
MGLL3.235e-4431.67monoglyceride lipase [Source:HGNC Symbol;Acc:HGNC:... [more]
MGLL2.417e-4233.60monoglyceride lipase [Source:HGNC Symbol;Acc:HGNC:... [more]
MGLL2.997e-3329.64monoglyceride lipase [Source:HGNC Symbol;Acc:HGNC:... [more]
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BLAST of Phospholipase YtpA vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phospholipase YtpA vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Phospholipase YtpA vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mgll2.871e-4733.70monoglyceride lipase [Source:ZFIN;Acc:ZDB-GENE-031... [more]
mgll2.191e-3233.96monoglyceride lipase [Source:ZFIN;Acc:ZDB-GENE-031... [more]
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BLAST of Phospholipase YtpA vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
slc35d12.503e-4531.10solute carrier family 35 member D1 [Source:Xenbase... [more]
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BLAST of Phospholipase YtpA vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mgll2.266e-5033.81monoglyceride lipase [Source:MGI Symbol;Acc:MGI:13... [more]
Mgll2.266e-5033.81monoglyceride lipase [Source:MGI Symbol;Acc:MGI:13... [more]
Mgll3.530e-5033.81monoglyceride lipase [Source:MGI Symbol;Acc:MGI:13... [more]
Mgll3.131e-4430.74monoglyceride lipase [Source:MGI Symbol;Acc:MGI:13... [more]
Mgll5.361e-3830.25monoglyceride lipase [Source:MGI Symbol;Acc:MGI:13... [more]
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BLAST of Phospholipase YtpA vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q8R431|MGLL_RAT1.827e-5134.04Monoglyceride lipase OS=Rattus norvegicus OX=10116... [more]
sp|O35678|MGLL_MOUSE1.585e-4933.81Monoglyceride lipase OS=Mus musculus OX=10090 GN=M... [more]
sp|Q99685|MGLL_HUMAN1.088e-4331.67Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MG... [more]
sp|O07427|MGLL_MYCTU1.882e-3030.22Monoacylglycerol lipase OS=Mycobacterium tuberculo... [more]
sp|A0QNZ7|MGLL_MYCS22.647e-2627.73Monoacylglycerol lipase OS=Mycolicibacterium smegm... [more]
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BLAST of Phospholipase YtpA vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A224Z3S06.909e-5433.57Lysophospholipase OS=Rhipicephalus zambeziensis OX... [more]
A0A1E1X7594.804e-5334.89Putative lysophospholipase (Fragment) OS=Amblyomma... [more]
A0A023FL546.968e-5335.25Putative lysophospholipase OS=Amblyomma cajennense... [more]
A0A1E1X7Q57.056e-5234.89Putative lysophospholipase (Fragment) OS=Amblyomma... [more]
A7T3M91.472e-5035.61Predicted protein OS=Nematostella vectensis OX=453... [more]
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BLAST of Phospholipase YtpA vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mgll1.220e-4732.25monoglyceride lipase [Source:NCBI gene;Acc:1030267... [more]
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BLAST of Phospholipase YtpA vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mgll1.124e-3629.46monoglyceride lipase [Source:ZFIN;Acc:ZDB-GENE-031... [more]
mgll9.987e-3630.62monoglyceride lipase [Source:ZFIN;Acc:ZDB-GENE-031... [more]
mgll4.877e-2328.68monoglyceride lipase [Source:ZFIN;Acc:ZDB-GENE-031... [more]
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BLAST of Phospholipase YtpA vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
YJU39.348e-1222.84Monoglyceride lipase (MGL); functional ortholog of... [more]
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BLAST of Phospholipase YtpA vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO294352.035e-5435.61Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Phospholipase YtpA vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mgll1.130e-4832.16monoglyceride lipase [Source:NCBI gene;Acc:1011698... [more]
mgll1.274e-4832.16monoglyceride lipase [Source:NCBI gene;Acc:1011698... [more]
mgll2.329e-4832.16monoglyceride lipase [Source:NCBI gene;Acc:1011698... [more]
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BLAST of Phospholipase YtpA vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000038738.10.000e+099.31SMESG000038738.1[more]
SMESG000038743.10.000e+092.04SMESG000038743.1[more]
SMESG000038748.13.875e-10649.65SMESG000038748.1[more]
SMESG000038746.14.511e-9848.94SMESG000038746.1[more]
SMESG000004815.11.675e-6233.79SMESG000004815.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003096 ID=SMED30003096|Name=Phospholipase YtpA|organism=Schmidtea mediterranea sexual|type=transcript|length=1085bp
AAATTCTGTTTGATTATAAATATATTTAGTTAGAATATTTTTAAAATTAT
GTCTGAAGAGAAAATAGAAAGTGATTATATTGTTAATGTTGATGGAATAA
AGTTGTTTAGAATGATTTATCATTCTACATCAGGTGCGACTAAAGCGATC
GTTTTTCTTTGTCATGGGTTATTTGAACATTGTCAATCATGTTATTATGA
ATCGATAATTAAACATTTGAATGCTATTGACTGTATGGTCGTAACTCATG
ATCACATGGGCCATGGACTTAGCAACGGTGACAGAGGAATGATTTATAAC
TTTGATTTGTTAGTACGCGATGTCCTTTATGATATCGATGATGTAGTTAA
TAAAATGCAAACAAAATCGCAACTTCCGATTTTTCTCTATGGTCATTCAT
TGGGTGGCCTTATTTCAGTTTATGCGACTTTAAAAAGGCCCACATTTTTC
AAGGGTATGTGCTTGGAAGCTCCGTTTTTGATCTTTCCAACAAATGTCAG
TAATGTGGCGCTTTATATGGGAAAGATTGTCAGTGTTTTGCTTCCAAAGA
TGCCACTTGGCTCAATTGATTACAATGATATATCTCGAGATGTTGAATAT
TTGGAACATGTACGCAAAGATAAATTGAGAATTCATTCTAGAATCCCTGC
TAAAACCGTTATTGAAATGCATAATGCTGTGGAAAACCTTAAAGGACAAA
TGGAAAATGTTACAACGCCATTTATTATCGGTCATGGTGAGGCAGATAAA
TTGTCCGACGTGGAAGGTAGCAAATTATTGGTAAAAAAATCTCCGATAAC
TGACAAAGTATTTATTCCATTTCCGAATGCCAAACACGAAATTAGAATGG
AACCTGGGAATGTTTCTTCAGATTTTGTGAAACAAATTGTTGAATGGTTG
CGAATTAAAATAAGCATTTGAAATTTTTTATCATATTGTTCAATTTTATA
TTTTCCCATTTTTTATTTAATTTGATCAACAGATTGATATTTTTACAGTT
TTTGATTTTGTTTTCTTTATTATTTAGTTATGATTGATATCTATAGGTGC
AGAGAACATTGGAAATAAAACTTTTTTTATAAATA
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protein sequence of SMED30003096-orf-1

>SMED30003096-orf-1 ID=SMED30003096-orf-1|Name=SMED30003096-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=291bp
MSEEKIESDYIVNVDGIKLFRMIYHSTSGATKAIVFLCHGLFEHCQSCYY
ESIIKHLNAIDCMVVTHDHMGHGLSNGDRGMIYNFDLLVRDVLYDIDDVV
NKMQTKSQLPIFLYGHSLGGLISVYATLKRPTFFKGMCLEAPFLIFPTNV
SNVALYMGKIVSVLLPKMPLGSIDYNDISRDVEYLEHVRKDKLRIHSRIP
AKTVIEMHNAVENLKGQMENVTTPFIIGHGEADKLSDVEGSKLLVKKSPI
TDKVFIPFPNAKHEIRMEPGNVSSDFVKQIVEWLRIKISI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0017000antibiotic biosynthetic process
GO:0006629lipid metabolic process
GO:0006631fatty acid metabolic process
GO:0006633fatty acid biosynthetic process
GO:0009966regulation of signal transduction
GO:0016042lipid catabolic process
GO:0019369arachidonic acid metabolic process
GO:0019433triglyceride catabolic process
GO:0046464acylglycerol catabolic process
GO:0050727regulation of inflammatory response
GO:0051930regulation of sensory perception of pain
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000018Category 4 cell
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR022742Hydrolase_4
IPR029058AB_hydrolase
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005811lipid particle
GO:0005829cytosol
GO:0016020membrane
GO:0019898extrinsic component of membrane
GO:0045202synapse
Vocabulary: molecular function
TermDefinition
GO:0008289lipid binding
GO:0016787hydrolase activity
GO:0047372acylglycerol lipase activity
GO:0052689carboxylic ester hydrolase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 30..269
e-value: 2.0E-51
score: 174.6
IPR029058Alpha/Beta hydrolase foldGENE3DG3DSA:3.40.50.1820coord: 2..288
e-value: 2.5E-54
score: 186.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILYSSF53474alpha/beta-Hydrolasescoord: 11..285
NoneNo IPR availablePANTHERPTHR11614PHOSPHOLIPASE-RELATEDcoord: 8..285
NoneNo IPR availablePANTHERPTHR11614:SF87MONOGLYCERIDE LIPASEcoord: 8..285
NoneNo IPR availableTMHMMTMhelixcoord: 109..128
NoneNo IPR availableTMHMMTMhelixcoord: 143..165