SMED30003093

Overview
NameSMED30003093
Smed IDSMED30003093
Length (bp)2649
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003093 (SMED30003093) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003093 (SMED30003093) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003093 (SMED30003093) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003093 (SMED30003093) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003093

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 24

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30003093SMESG000056838.1 Contig3451newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003093SMESG000056838.1 Contig3451uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003093SMESG000027907.1 Contig3451newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003093SMESG000027907.1 Contig3451uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
neoblastSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynxSMED30003093SMESG000056845.1 SMESG000044775.1 SMESG000007087.1 SMESG000007085.1 SMESG000056847.1 SMESG000056838.1 SMESG000044770.1 SMESG000007140.1 SMESG000044776.1 SMESG000044783.1 SMESG000038659.1 SMESG000030652.1 SMESG000025467.1 SMESG000013956.1 SMESG000056843.1 SMESG000044762.1 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
intestinal phagocyteSMED30003093SMESG000056845.1 SMESG000044775.1 SMESG000007087.1 SMESG000007085.1 SMESG000056847.1 SMESG000056838.1 SMESG000044770.1 SMESG000007140.1 SMESG000044776.1 SMESG000044783.1 SMESG000038659.1 SMESG000030652.1 SMESG000025467.1 SMESG000013956.1 SMESG000056843.1 SMESG000044762.1 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30003093SMESG000056845.1 SMESG000044775.1 SMESG000007087.1 SMESG000007085.1 SMESG000056847.1 SMESG000056838.1 SMESG000044770.1 SMESG000007140.1 SMESG000044776.1 SMESG000044783.1 SMESG000038659.1 SMESG000030652.1 SMESG000025467.1 SMESG000013956.1 SMESG000056843.1 SMESG000044762.1 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cholinergic neuronSMED30003093SMESG000056845.1 SMESG000044775.1 SMESG000007087.1 SMESG000007085.1 SMESG000056847.1 SMESG000056838.1 SMESG000044770.1 SMESG000007140.1 SMESG000044776.1 SMESG000044783.1 SMESG000038659.1 SMESG000030652.1 SMESG000025467.1 SMESG000013956.1 SMESG000056843.1 SMESG000044762.1 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
GABAergic neuronSMED30003093SMESG000056845.1 SMESG000044775.1 SMESG000007087.1 SMESG000007085.1 SMESG000056847.1 SMESG000056838.1 SMESG000044770.1 SMESG000007140.1 SMESG000044776.1 SMESG000044783.1 SMESG000038659.1 SMESG000030652.1 SMESG000025467.1 SMESG000013956.1 SMESG000056843.1 SMESG000044762.1 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30003093SMESG000056845.1 SMESG000044775.1 SMESG000007087.1 SMESG000007085.1 SMESG000056847.1 SMESG000056838.1 SMESG000044770.1 SMESG000007140.1 SMESG000044776.1 SMESG000044783.1 SMESG000038659.1 SMESG000030652.1 SMESG000025467.1 SMESG000013956.1 SMESG000056843.1 SMESG000044762.1 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
secretory systemSMED30003093SMESG000056845.1 SMESG000044775.1 SMESG000007087.1 SMESG000007085.1 SMESG000056847.1 SMESG000056838.1 SMESG000044770.1 SMESG000007140.1 SMESG000044776.1 SMESG000044783.1 SMESG000038659.1 SMESG000030652.1 SMESG000025467.1 SMESG000013956.1 SMESG000056843.1 SMESG000044762.1 dd_Smed_v6_1137_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30003093SMESG000056847.1 SMESG000056843.1 SMESG000056838.1 SMESG000044770.1 SMESG000044762.1 SMESG000007140.1 dd_Smed_v4_1137_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003093 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001626:27561..43986 -12748
Homology
BLAST of SMED30003093 vs. Planmine SMEST
Match: SMESG000056843.1 (SMESG000056843.1)

HSP 1 Score: 365.54 bits (937), Expect = 2.858e-120
Identity = 192/223 (86.10%), Postives = 201/223 (90.13%), Query Frame = 1
Query:  253 IGQFIKI-----LHYNLFGLIESFYLKNSVKLFKVQLNKDQVNNFPFLSKFDRSHDNPFYLTIVVKKDFVEKCREKLEFLSEFPIINQECIEYKIPNLVKRFRKNELIKVVKENNVKYFNVIDCVFIHGFSCLSVTEPILGSSLEFVEEVHASKVDDYELELKYFQVFIDPKSILSFEKYEKVDLSIGNHVEFKSNDCNVPFRGIVQYIGIANKILPSCIVQL 906
            IG+ IKI     L  N    ++++  K+  K+  +QLN +   NFPFLSKFDRSHDNPFYLTIVVKKDFVEKCREKLEFLSEFPIINQECIEYKIPNLVKRFRKNELIKVVKENNVKYFNVIDCVFIHGFSCLSVTEPILGSSLEFVEEVHASKVDDYELELKYFQVFIDPKSILSFEKYEKVDLSIGNHVEFKSNDCNVPFRGIVQYIGIANKILPSCIVQL
Sbjct:   31 IGKLIKIEEKYYLAINKEDRVKAYNNKSFQKISNIQLN-ELYENFPFLSKFDRSHDNPFYLTIVVKKDFVEKCREKLEFLSEFPIINQECIEYKIPNLVKRFRKNELIKVVKENNVKYFNVIDCVFIHGFSCLSVTEPILGSSLEFVEEVHASKVDDYELELKYFQVFIDPKSILSFEKYEKVDLSIGNHVEFKSNDCNVPFRGIVQYIGIANKILPSCIVQL 252          
BLAST of SMED30003093 vs. Planmine SMEST
Match: SMESG000056843.1 (SMESG000056843.1)

HSP 1 Score: 364.77 bits (935), Expect = 3.439e-120
Identity = 192/223 (86.10%), Postives = 201/223 (90.13%), Query Frame = 1
Query:  253 IGQFIKI-----LHYNLFGLIESFYLKNSVKLFKVQLNKDQVNNFPFLSKFDRSHDNPFYLTIVVKKDFVEKCREKLEFLSEFPIINQECIEYKIPNLVKRFRKNELIKVVKENNVKYFNVIDCVFIHGFSCLSVTEPILGSSLEFVEEVHASKVDDYELELKYFQVFIDPKSILSFEKYEKVDLSIGNHVEFKSNDCNVPFRGIVQYIGIANKILPSCIVQL 906
            IG+ IKI     L  N    ++++  K+  K+  +QLN +   NFPFLSKFDRSHDNPFYLTIVVKKDFVEKCREKLEFLSEFPIINQECIEYKIPNLVKRFRKNELIKVVKENNVKYFNVIDCVFIHGFSCLSVTEPILGSSLEFVEEVHASKVDDYELELKYFQVFIDPKSILSFEKYEKVDLSIGNHVEFKSNDCNVPFRGIVQYIGIANKILPSCIVQL
Sbjct:   22 IGKLIKIEEKYYLAINKEDRVKAYNNKSFQKISNIQLN-ELYENFPFLSKFDRSHDNPFYLTIVVKKDFVEKCREKLEFLSEFPIINQECIEYKIPNLVKRFRKNELIKVVKENNVKYFNVIDCVFIHGFSCLSVTEPILGSSLEFVEEVHASKVDDYELELKYFQVFIDPKSILSFEKYEKVDLSIGNHVEFKSNDCNVPFRGIVQYIGIANKILPSCIVQL 243          
BLAST of SMED30003093 vs. Planmine SMEST
Match: SMESG000056838.1 (SMESG000056838.1)

HSP 1 Score: 288.886 bits (738), Expect = 3.421e-101
Identity = 157/163 (96.32%), Postives = 158/163 (96.93%), Query Frame = 2
Query: 1787 DDCCELEGHRPIKLHQNSAIDENSICCVMANKISSIQIENLIEKINDNQNAFCLWSGLVNDVEVVGVEMKEPIDNGVNGVFRGLKLFNCEQRRGALFRAXXXXXXXXXXXXXKPIPEVFCIFKEIVINSSVKYYFKTGDKIKDLYGTVKWTGADNENR-YFGL 2272
            D  C LEGHRPIKLHQNSAIDENSICCVMANKISSIQIENLIEKINDNQNA CLWSGLVNDVEVVGVEMKEPIDNGVNGVFRGLKLFNCEQRRGALFRAEEFI+IPTEFFETKPIPEVFCIFKEIVINSSVKYYFKTGDKIKDLYGTVKWTGADNENR YFGL
Sbjct:   13 DILCLLEGHRPIKLHQNSAIDENSICCVMANKISSIQIENLIEKINDNQNAICLWSGLVNDVEVVGVEMKEPIDNGVNGVFRGLKLFNCEQRRGALFRAEEFIQIPTEFFETKPIPEVFCIFKEIVINSSVKYYFKTGDKIKDLYGTVKWTGADNENRYYFGL 175          

HSP 2 Score: 100.908 bits (250), Expect = 3.421e-101
Identity = 47/48 (97.92%), Postives = 48/48 (100.00%), Query Frame = 3
Query: 2292 YFGLELEKRISIGTNGLFNGQQLFICPNGYGVLAPIDRVAIDIRFIER 2435
            YFGLEL+KRISIGTNGLFNGQQLFICPNGYGVLAPIDRVAIDIRFIER
Sbjct:  172 YFGLELDKRISIGTNGLFNGQQLFICPNGYGVLAPIDRVAIDIRFIER 219          
BLAST of SMED30003093 vs. Planmine SMEST
Match: SMESG000056845.1 (SMESG000056845.1)

HSP 1 Score: 228.794 bits (582), Expect = 9.276e-62
Identity = 136/307 (44.30%), Postives = 181/307 (58.96%), Query Frame = 1
Query:   28 GTCEWLGLINGKEYVGI-LFDEITHR------RTRSDQISEILQIDYRIQ----MIPIQCVSISIFEARNKKFTQSDSIFLDDTYKIGQFIKILHYNLFGLIESFYLKNSVKLFKVQLNKDQVNNFPFLSKFDRSHDNPFYLTIVVKKDFVEKCREKLEFLSEFPIINQECIEYKIPNLVKRFRKNELIKVVKENNVKYFNVIDCVFIHGFSCLSVTEPILGSSLEFVEEVHASKVDDYELELKYFQ-VFIDPKSILSFEKYEKVDLSIGNHVEFKSNDCNVPFRGIVQYIGIANKILPSCIVQLEN 912
            GT +W GL+N      + L  E  +       RTR  +  + L I    Q     +PI  +   IF  +N+K     S+   D Y +GQF+KIL ++LFG IE F++       +VQL KDQ+   PF  + DR+  N  Y T+++ KD + KC E LEF +E  +IN+ECIEY+IP  ++ FR  +LIK  K  NVKYF V +   I+G SCLSV E ILG + E++ E  A KVD+ ELE+KYFQ VFIDPKSI SFEK E  D  IGN VEF S D    + G ++  GIANK +PSC+VQ+ N
Sbjct:  750 GTLKWYGLMNNVPVCAVQLLQEHENGTNGRFWRTRLFECRDKLAIATLTQNLSPSMPIDFIK-DIFVEKNQKEFSRGSVRTGDIYHVGQFVKILPFDLFGQIECFHVVYEEMFVQVQLTKDQIKRLPFHQRIDRAASNAIYSTVILLKDSIAKCSENLEFFNESSLINKECIEYRIPRPMETFRTGDLIKAGKTGNVKYFIVNNRFIINGISCLSVMESILGEAEEYLPESEAMKVDNSELEMKYFQGVFIDPKSIRSFEKCEINDFCIGNRVEFMSMDRKSSYNGRIKSTGIANKTIPSCVVQMIN 1055          

HSP 2 Score: 78.9518 bits (193), Expect = 1.200e-14
Identity = 35/59 (59.32%), Postives = 45/59 (76.27%), Query Frame = 1
Query:    1 VFCYLLKDGGTCEWLGLINGKEYVGILFDEITHRRTRSDQISEILQIDYRIQMIPIQCV 177
            VFC  LK GG C+W+GLINGK YVGI FDEI  R  R +++S IL+I+ RI+ IP++CV
Sbjct:  566 VFCQQLKAGGKCKWMGLINGKGYVGIEFDEIHRRAERFNELSRILKIEGRIRFIPLECV 624          

HSP 3 Score: 77.0258 bits (188), Expect = 5.172e-14
Identity = 49/136 (36.03%), Postives = 64/136 (47.06%), Query Frame = 2
Query: 1622 KEFFKLKWRGLVDKRYCLGCEINFQTDFGTNGTFGQEHLFDCKENQGVLIPL-DYVDDCCELEGHRPIKLHQNSAIDENSICCVMANKISSIQIENLIEKINDNQNAFCL---------WSGLVNDVEVVGVEMKE 1999
            K F KL W+G ++   CLGCE+      GT GTFG+  LF C  ++GVLIP+ D V  C   +    + L  N  + EN+ICC          +E L   I  N   FC          W GL+N    VG+E  E
Sbjct:  467 KSFVKLFWKGKINNINCLGCELPEYWREGTEGTFGRHKLFTCSYSRGVLIPINDAVKSCESADESNDLPLTDNDELTENNICCFEP-------LEKLSNDIEVNSRVFCQQLKAGGKCKWMGLINGKGYVGIEFDE 595          

HSP 4 Score: 72.4034 bits (176), Expect = 1.475e-12
Identity = 36/65 (55.38%), Postives = 45/65 (69.23%), Query Frame = 1
Query:    1 VFCYLLKDGGTCEWLGLINGKEYVGILFDE-ITHRRTRSDQISEILQIDYRIQMIPIQCVSISIF 192
            VFC  LK GG C W+GLINGK YVGI FDE I    +  + ISEIL+I  +I+MIP+Q V  +I+
Sbjct:  367 VFCQQLKAGGKCNWMGLINGKGYVGIEFDEIIPDGNSTQNSISEILKIQGQIRMIPVQSVLPTIY 431          

HSP 5 Score: 57.7658 bits (138), Expect = 3.725e-8
Identity = 38/113 (33.63%), Postives = 52/113 (46.02%), Query Frame = 2
Query: 1706 GTNGTFGQEHLFDCKENQGVLIPL-DYVDDCCELEGHRPIKLHQNSAIDENSICCVMANKISSIQIENLIEKINDNQNAFCL---------WSGLVNDVEVVGVEMKEPIDNG 2014
            GT GTFG+  LF C  ++GVLIP+ D V  C   +    + L  N  +  N+ICC          ++ L + I  N   FC          W GL+N    VG+E  E I +G
Sbjct:  296 GTEGTFGRYKLFTCSYDKGVLIPINDAVKSCESADESNHLPLADNDELTANNICCFEP-------LDKLSKDIKVNSRVFCQQLKAGGKCNWMGLINGKGYVGIEFDEIIPDG 401          
BLAST of SMED30003093 vs. Planmine SMEST
Match: SMESG000056845.1 (SMESG000056845.1)

HSP 1 Score: 228.794 bits (582), Expect = 9.704e-62
Identity = 136/307 (44.30%), Postives = 181/307 (58.96%), Query Frame = 1
Query:   28 GTCEWLGLINGKEYVGI-LFDEITHR------RTRSDQISEILQIDYRIQ----MIPIQCVSISIFEARNKKFTQSDSIFLDDTYKIGQFIKILHYNLFGLIESFYLKNSVKLFKVQLNKDQVNNFPFLSKFDRSHDNPFYLTIVVKKDFVEKCREKLEFLSEFPIINQECIEYKIPNLVKRFRKNELIKVVKENNVKYFNVIDCVFIHGFSCLSVTEPILGSSLEFVEEVHASKVDDYELELKYFQ-VFIDPKSILSFEKYEKVDLSIGNHVEFKSNDCNVPFRGIVQYIGIANKILPSCIVQLEN 912
            GT +W GL+N      + L  E  +       RTR  +  + L I    Q     +PI  +   IF  +N+K     S+   D Y +GQF+KIL ++LFG IE F++       +VQL KDQ+   PF  + DR+  N  Y T+++ KD + KC E LEF +E  +IN+ECIEY+IP  ++ FR  +LIK  K  NVKYF V +   I+G SCLSV E ILG + E++ E  A KVD+ ELE+KYFQ VFIDPKSI SFEK E  D  IGN VEF S D    + G ++  GIANK +PSC+VQ+ N
Sbjct:  779 GTLKWYGLMNNVPVCAVQLLQEHENGTNGRFWRTRLFECRDKLAIATLTQNLSPSMPIDFIK-DIFVEKNQKEFSRGSVRTGDIYHVGQFVKILPFDLFGQIECFHVVYEEMFVQVQLTKDQIKRLPFHQRIDRAASNAIYSTVILLKDSIAKCSENLEFFNESSLINKECIEYRIPRPMETFRTGDLIKAGKTGNVKYFIVNNRFIINGISCLSVMESILGEAEEYLPESEAMKVDNSELEMKYFQGVFIDPKSIRSFEKCEINDFCIGNRVEFMSMDRKSSYNGRIKSTGIANKTIPSCVVQMIN 1084          

HSP 2 Score: 78.9518 bits (193), Expect = 1.260e-14
Identity = 35/59 (59.32%), Postives = 45/59 (76.27%), Query Frame = 1
Query:    1 VFCYLLKDGGTCEWLGLINGKEYVGILFDEITHRRTRSDQISEILQIDYRIQMIPIQCV 177
            VFC  LK GG C+W+GLINGK YVGI FDEI  R  R +++S IL+I+ RI+ IP++CV
Sbjct:  566 VFCQQLKAGGKCKWMGLINGKGYVGIEFDEIHRRAERFNELSRILKIEGRIRFIPLECV 624          

HSP 3 Score: 77.0258 bits (188), Expect = 5.159e-14
Identity = 49/136 (36.03%), Postives = 64/136 (47.06%), Query Frame = 2
Query: 1622 KEFFKLKWRGLVDKRYCLGCEINFQTDFGTNGTFGQEHLFDCKENQGVLIPL-DYVDDCCELEGHRPIKLHQNSAIDENSICCVMANKISSIQIENLIEKINDNQNAFCL---------WSGLVNDVEVVGVEMKE 1999
            K F KL W+G ++   CLGCE+      GT GTFG+  LF C  ++GVLIP+ D V  C   +    + L  N  + EN+ICC          +E L   I  N   FC          W GL+N    VG+E  E
Sbjct:  467 KSFVKLFWKGKINNINCLGCELPEYWREGTEGTFGRHKLFTCSYSRGVLIPINDAVKSCESADESNDLPLTDNDELTENNICCFEP-------LEKLSNDIEVNSRVFCQQLKAGGKCKWMGLINGKGYVGIEFDE 595          

HSP 4 Score: 72.4034 bits (176), Expect = 1.522e-12
Identity = 36/65 (55.38%), Postives = 45/65 (69.23%), Query Frame = 1
Query:    1 VFCYLLKDGGTCEWLGLINGKEYVGILFDE-ITHRRTRSDQISEILQIDYRIQMIPIQCVSISIF 192
            VFC  LK GG C W+GLINGK YVGI FDE I    +  + ISEIL+I  +I+MIP+Q V  +I+
Sbjct:  367 VFCQQLKAGGKCNWMGLINGKGYVGIEFDEIIPDGNSTQNSISEILKIQGQIRMIPVQSVLPTIY 431          

HSP 5 Score: 57.7658 bits (138), Expect = 3.740e-8
Identity = 38/113 (33.63%), Postives = 52/113 (46.02%), Query Frame = 2
Query: 1706 GTNGTFGQEHLFDCKENQGVLIPL-DYVDDCCELEGHRPIKLHQNSAIDENSICCVMANKISSIQIENLIEKINDNQNAFCL---------WSGLVNDVEVVGVEMKEPIDNG 2014
            GT GTFG+  LF C  ++GVLIP+ D V  C   +    + L  N  +  N+ICC          ++ L + I  N   FC          W GL+N    VG+E  E I +G
Sbjct:  296 GTEGTFGRYKLFTCSYDKGVLIPINDAVKSCESADESNHLPLADNDELTANNICCFEP-------LDKLSKDIKVNSRVFCQQLKAGGKCNWMGLINGKGYVGIEFDEIIPDG 401          

HSP 6 Score: 55.8398 bits (133), Expect = 1.453e-7
Identity = 42/151 (27.81%), Postives = 73/151 (48.34%), Query Frame = 2
Query: 1634 KLKWRGLVDKRYCLGCEINFQTDFGTNGTFGQEHLFDCKENQGVLIPLDYVDDC----CELEGHRPIKLHQ--NSAIDENSICCVMANKISSIQIENLIEKINDNQN--AFCLWSGLVNDVEVVGVEMKEPIDNGVNGVFRGLKLFNCEQR 2062
            +LKW G ++   C+G ++  + +F  +G   Q++ FDC E  G++  L  ++      C L    P K  +   +  DE    C+ A +I    + N ++ +N   N      W GL+N+V V  V++ +  +NG NG F   +LF C  +
Sbjct:  675 ELKWIGKINNEKCVGLDMEDEMEFTMDGIINQDNRFDCMEKHGLITVLSNINMTIMFICYLWIINPDKYLRCKETFGDE----CIKAQRIKKDGMVN-VKLLNGQANLKGTLKWYGLMNNVPVCAVQLLQEHENGTNGRFWRTRLFECRDK 820          
The following BLAST results are available for this feature:
BLAST of SMED30003093 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003093 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000056843.12.858e-12086.10SMESG000056843.1[more]
SMESG000056843.13.439e-12086.10SMESG000056843.1[more]
SMESG000056838.13.421e-10196.32SMESG000056838.1[more]
SMESG000056845.19.276e-6244.30SMESG000056845.1[more]
SMESG000056845.19.704e-6244.30SMESG000056845.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003093 ID=SMED30003093|Name=SMED30003093|organism=Schmidtea mediterranea sexual|type=transcript|length=2649bp
GTATTTTGTTATTTATTAAAAGATGGTGGGACGTGCGAATGGCTGGGATT
AATCAATGGAAAAGAATATGTTGGGATATTATTTGATGAAATTACTCACA
GACGCACCAGGAGTGATCAAATATCAGAAATATTGCAGATAGATTATAGA
ATCCAAATGATCCCCATTCAATGTGTTTCAATTTCCATATTTGAGGCAAG
AAATAAAAAATTCACCCAATCCGATTCTATTTTCTTGGATGATACTTATA
AAATTGGTCAATTTATCAAAATTTTACACTACAACTTATTTGGTCTCATT
GAAAGCTTTTATTTGAAAAACAGTGTAAAATTATTTAAAGTCCAATTAAA
CAAAGATCAAGTCAATAATTTTCCTTTTCTATCAAAATTCGATCGTTCCC
ATGACAATCCATTTTATTTGACAATTGTTGTGAAAAAGGATTTTGTAGAA
AAATGTCGAGAAAAACTTGAATTCCTCAGTGAATTTCCAATAATAAACCA
AGAATGTATTGAGTATAAGATTCCAAATCTAGTGAAAAGGTTTCGAAAGA
ATGAGCTCATTAAAGTTGTGAAAGAAAACAATGTGAAATATTTCAATGTT
ATAGACTGTGTCTTCATACATGGATTTTCCTGTTTATCTGTTACTGAACC
TATTCTTGGATCTTCCTTGGAGTTTGTGGAAGAAGTTCATGCTTCAAAAG
TGGATGATTATGAATTGGAATTGAAATATTTTCAAGTTTTTATTGACCCT
AAATCAATTTTATCATTTGAAAAATATGAGAAAGTCGATTTAAGTATTGG
AAATCATGTGGAATTCAAGTCGAATGATTGCAATGTTCCCTTCAGAGGAA
TAGTTCAATATATTGGAATTGCCAACAAAATTCTGCCTTCGTGCATTGTT
CAACTGGAAAACAAAACAAAGTTTTTGATAGAAAAATATATTCCAAATAA
AAATACAATCCAATTCGACAAAAATTCAAAAATACTTCAAAACCCGAACG
CTTTTAAACAAACGTAGACCAGGAAAAAATTACAAAATTCAACTAATTTA
ATCCAACACAGAAAATCTCGATTTATACTCGATAACAAAAATCTCTTCGA
AAATCGAAAGGCGAACAATTACAAGCTAAAAGTGAGCGCACTAGTGTTTT
TGATTTGGAAAAACCTGTCTAACTTTTGAAAAATGGAAATAAGAAATAAA
TTCTGGAGAAAATCACATTATAACAAGAAACCTGACAAAACGTCTAAGCA
TCTAAAATGATATGTAGAATAAATTAGGTATCAGGAAACTGTGTTCACGA
TTTGTTCCACGATTTCTTATGGGTAACATGCAACAAAGAAAAATTCTTCC
GAGGAAATTACTGAAAAAATATCCGGATATTCATTAAATGAAAACCTTGC
GTTCCAAAGCTTATGTACCACTGAGGATGGCCCTACTTATATAATTTCCT
CTTATTTACTAACTAGGCTAGGTTTGTTTGTTGTATTTTTTTTTAAGTGA
TTGATGTTTAGATTTGTCCGAGACGGTAAATGGCATTCAAGTAATAAATT
CAAAATTTATCAAAAATATAAAAGGAAACAAAATACAAGAGAAATTTATA
ATTTTCAAGAGTAATAATAAAAAAGAATTTTTCAAATTGAAATGGAGGGG
TCTTGTTGATAAGCGATATTGTTTAGGCTGTGAAATTAACTTTCAAACAG
ATTTCGGGACTAATGGTACATTTGGACAGGAACATCTGTTTGATTGTAAA
GAAAATCAAGGTGTTTTGATTCCGTTGGATTACGTGGACGATTGCTGTGA
ATTAGAAGGGCATAGACCAATCAAATTGCATCAAAACAGCGCAATAGACG
AAAACAGTATTTGTTGTGTCATGGCTAATAAAATTTCAAGCATTCAAATT
GAAAATCTAATAGAAAAAATTAATGACAATCAGAATGCCTTTTGTTTATG
GAGTGGCCTTGTGAATGATGTTGAAGTTGTTGGAGTCGAAATGAAAGAGC
CAATCGATAATGGGGTAAATGGAGTATTTCGTGGACTAAAGTTATTCAAT
TGTGAACAGCGGCGTGGAGCATTGTTTAGAGCTGAAGAATTCATAGAAAT
TCCAACTGAGTTCTTTGAAACTAAACCGATTCCTGAAGTGTTTTGCATAT
TTAAAGAAATTGTCATAAATTCATCTGTTAAATATTATTTCAAAACTGGT
GATAAAATTAAAGATCTATATGGTACTGTTAAATGGACTGGGGCGGATAA
CGAAAATCGGTACTTTGGATTACTTTGGATTACTTTTGGATTACTTTGGA
TTAGAATTGGAAAAAAGAATTAGTATTGGGACAAATGGATTATTTAATGG
TCAACAGTTATTTATTTGCCCTAATGGTTATGGGGTATTGGCACCAATAG
ATCGTGTTGCTATAGATATCCGATTTATCGAAAGAAGTGAGGATTAATTT
TTTGTCATGATAAATATTAAATATTTATTTATCTTCATTTCTTTTCAGGC
TATAGATCAATATTTGTATAAATCAATATTTAACTAGAATATTGATTTAT
ATAGTCTAAGAAGCGATTAAGGTCAAAGAAATTGAACTTGAAAATAGATT
AATTTTTAGCATTTACAAAAATTGGAAAATTATAGAGTAGATTTTTAAC
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protein sequence of SMED30003093-orf-1

>SMED30003093-orf-1 ID=SMED30003093-orf-1|Name=SMED30003093-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=151bp
MANKISSIQIENLIEKINDNQNAFCLWSGLVNDVEVVGVEMKEPIDNGVN
GVFRGLKLFNCEQRRGALFRAEEFIEIPTEFFETKPIPEVFCIFKEIVIN
SSVKYYFKTGDKIKDLYGTVKWTGADNENRYFGLLWITFGLLWIRIGKKN
*
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protein sequence of SMED30003093-orf-2

>SMED30003093-orf-2 ID=SMED30003093-orf-2|Name=SMED30003093-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=339bp
VFCYLLKDGGTCEWLGLINGKEYVGILFDEITHRRTRSDQISEILQIDYR
IQMIPIQCVSISIFEARNKKFTQSDSIFLDDTYKIGQFIKILHYNLFGLI
ESFYLKNSVKLFKVQLNKDQVNNFPFLSKFDRSHDNPFYLTIVVKKDFVE
KCREKLEFLSEFPIINQECIEYKIPNLVKRFRKNELIKVVKENNVKYFNV
IDCVFIHGFSCLSVTEPILGSSLEFVEEVHASKVDDYELELKYFQVFIDP
KSILSFEKYEKVDLSIGNHVEFKSNDCNVPFRGIVQYIGIANKILPSCIV
QLENKTKFLIEKYIPNKNTIQFDKNSKILQNPNAFKQT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000463cholinergic neuron
PLANA:0000464GABAergic neuron
PLANA:0000484secretory system
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR000938CAP-Gly_domain