GTP-binding protein-like protein

Overview
NameGTP-binding protein-like protein
Smed IDSMED30003086
Length (bp)826
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of GTP-binding protein-like protein (SMED30003086) t-SNE clustered cells

Violin plots show distribution of expression levels for GTP-binding protein-like protein (SMED30003086) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of GTP-binding protein-like protein (SMED30003086) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for GTP-binding protein-like protein (SMED30003086) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30003086

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30003086h1SMcG0003670 Contig41380uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003086h1SMcG0003670 Contig41380newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30003086h1SMcG0003670 dd_Smed_v4_589_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30003086h1SMcG0003670 dd_Smed_v4_589_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30003086h1SMcG0003670 dd_Smed_v4_589_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30003086h1SMcG0003670 dd_Smed_v6_589_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of GTP-binding protein-like protein vs. Ensembl Human
Match: SAR1A (secretion associated Ras related GTPase 1A [Source:HGNC Symbol;Acc:HGNC:10534])

HSP 1 Score: 269.626 bits (688), Expect = 4.327e-91
Identity = 126/198 (63.64%), Postives = 158/198 (79.80%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+LGNKID+  A++EE LR   G+    TGKGNV+ +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNA-RPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Human
Match: SAR1A (secretion associated Ras related GTPase 1A [Source:HGNC Symbol;Acc:HGNC:10534])

HSP 1 Score: 269.626 bits (688), Expect = 4.327e-91
Identity = 126/198 (63.64%), Postives = 158/198 (79.80%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+LGNKID+  A++EE LR   G+    TGKGNV+ +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNA-RPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Human
Match: SAR1A (secretion associated Ras related GTPase 1A [Source:HGNC Symbol;Acc:HGNC:10534])

HSP 1 Score: 269.626 bits (688), Expect = 4.327e-91
Identity = 126/198 (63.64%), Postives = 158/198 (79.80%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+LGNKID+  A++EE LR   G+    TGKGNV+ +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNA-RPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Human
Match: SAR1A (secretion associated Ras related GTPase 1A [Source:HGNC Symbol;Acc:HGNC:10534])

HSP 1 Score: 269.626 bits (688), Expect = 4.327e-91
Identity = 126/198 (63.64%), Postives = 158/198 (79.80%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+LGNKID+  A++EE LR   G+    TGKGNV+ +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNA-RPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Human
Match: SAR1B (secretion associated Ras related GTPase 1B [Source:HGNC Symbol;Acc:HGNC:10535])

HSP 1 Score: 266.544 bits (680), Expect = 6.087e-90
Identity = 124/198 (62.63%), Postives = 159/198 (80.30%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS ++DW     S VL +LGL+KK+G+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +I+GIVFLVD  D +RL ES++EL  L+ D+ I+NVP+L+LGNKID+  A++EE LR   G+    TGKG++S +EL   RPLE+FMCS+LKRQGYGEGFRW+A Y+
Sbjct:    1 MSFIFDWIYSGFSSVLQFLGLYKKTGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETIANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGSISLKELNA-RPLEVFMCSVLKRQGYGEGFRWMAQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Celegans
Match: sar-1 (GTP-binding protein SAR1 [Source:UniProtKB/Swiss-Prot;Acc:Q23445])

HSP 1 Score: 266.929 bits (681), Expect = 1.646e-90
Identity = 122/198 (61.62%), Postives = 163/198 (82.32%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS LWDWF+G    VL+ LGL  K G+LVFLGLDNAGKTT+LHMLKDDR+A H PTLHPT++++ +GG+ FTTYDLGGH QAR++WK+Y  ++D +VFL+D  D +R+ ES+ EL+ L+QD++I++VP+L+LGNKIDK GAL+E+ L++ L IQ++ TGKG+VSR E+   RP+E+FMCS+L+RQGYGEG RWL  YL
Sbjct:    1 MSFLWDWFNG----VLNMLGLANKKGKLVFLGLDNAGKTTLLHMLKDDRIAQHVPTLHPTSEQMSLGGISFTTYDLGGHAQARRVWKDYFPAVDAVVFLIDVADAERMQESRVELESLLQDEQIASVPVLILGNKIDKPGALSEDQLKWQLNIQHMCTGKGDVSRNEMAS-RPMEVFMCSVLQRQGYGEGIRWLGQYL 193          
BLAST of GTP-binding protein-like protein vs. Ensembl Celegans
Match: arl-1 (ADP-ribosylation factor-like protein 1 [Source:UniProtKB/Swiss-Prot;Acc:Q20758])

HSP 1 Score: 106.686 bits (265), Expect = 3.717e-28
Identity = 64/187 (34.22%), Postives = 100/187 (53.48%), Query Frame = 3
Query:   90 GVLSYL-GLW-KKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            GV+SY  GL+  +  R++ LGLD AGKTT+L+ L+   + T  PT+    +++    +KF  +DLGG    R  W+ Y ++ D I+++VD+ D+ R+  S++EL  ++Q+DE+    L VL NK D  G L E  +   LG+  L               R +++F  S  K +G      WLAN L
Sbjct:    3 GVMSYFRGLFGAREMRILILGLDGAGKTTILYRLQVGEVVTTIPTIGFNVEQVEYKNLKFQVWDLGGQTSIRPYWRCYYANTDAIIYVVDSADRDRVGISRQELATMLQEDELQGAVLAVLANKQDIAGCLTETEVYKALGLDAL-------------RNRTIQIFKTSASKGEGLDPAMDWLANQL 176          
BLAST of GTP-binding protein-like protein vs. Ensembl Celegans
Match: arl-1 (ADP-ribosylation factor-like protein 1 [Source:UniProtKB/Swiss-Prot;Acc:Q20758])

HSP 1 Score: 106.686 bits (265), Expect = 3.717e-28
Identity = 64/187 (34.22%), Postives = 100/187 (53.48%), Query Frame = 3
Query:   90 GVLSYL-GLW-KKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            GV+SY  GL+  +  R++ LGLD AGKTT+L+ L+   + T  PT+    +++    +KF  +DLGG    R  W+ Y ++ D I+++VD+ D+ R+  S++EL  ++Q+DE+    L VL NK D  G L E  +   LG+  L               R +++F  S  K +G      WLAN L
Sbjct:    3 GVMSYFRGLFGAREMRILILGLDGAGKTTILYRLQVGEVVTTIPTIGFNVEQVEYKNLKFQVWDLGGQTSIRPYWRCYYANTDAIIYVVDSADRDRVGISRQELATMLQEDELQGAVLAVLANKQDIAGCLTETEVYKALGLDAL-------------RNRTIQIFKTSASKGEGLDPAMDWLANQL 176          
BLAST of GTP-binding protein-like protein vs. Ensembl Celegans
Match: arf-3 (ADP-Ribosylation Factor related; ADP-ribosylation factor related (20.5 kD) (Arf-3) [Source:UniProtKB/TrEMBL;Acc:G5EFK4])

HSP 1 Score: 106.686 bits (265), Expect = 4.176e-28
Identity = 58/178 (32.58%), Postives = 99/178 (55.62%), Query Frame = 3
Query:  117 KKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYG--EGFRWLANYL 644
            K+  R++ +GLD AGKTT+L+ LK   + T  PT+    + +    + FT +D+GG ++ R +W++Y  +  G++F+VD+ D++R+ ES++EL  ++ +DE+ +  LLV  NK D   A+    L   LG+ NL               R  + ++ +    QG+G  EG  WL+N L
Sbjct:   15 KRQVRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDKERIEESREELHKMLNEDELRDATLLVFANKQDLPNAMTAAELTDKLGLHNL---------------RSRQWYIQATCATQGHGLYEGLDWLSNQL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Celegans
Match: arl-5 (ADP-ribosylation factor-like protein 5 [Source:UniProtKB/Swiss-Prot;Acc:P34212])

HSP 1 Score: 102.449 bits (254), Expect = 1.535e-26
Identity = 53/175 (30.29%), Postives = 89/175 (50.86%), Query Frame = 3
Query:  120 KSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            K  +++ +GLDNAGKTT+L+           PT+    +E+    + F  +D+GG E  RK W  Y    D ++ ++D+ D  R+P  +++L  ++Q ++++   +LVL NK D  GA+N   +   LG+Q             L G R  ++  C  +K +G  E   W+AN L
Sbjct:   16 KKYKIIVVGLDNAGKTTILYNYVTKDQVETKPTIGSNVEEVSYRNLDFVIWDIGGQESLRKSWSTYYVQTDVVIVVIDSSDTTRIPIMKEQLHNMLQHEDLARAHILVLANKQDLPGAMNPAEVSTQLGLQT------------LRGARKWQINGCCAVKGEGLPEALEWIANNL 178          
BLAST of GTP-binding protein-like protein vs. Ensembl Fly
Match: Sar1 (gene:FBgn0038947 transcript:FBtr0084208)

HSP 1 Score: 270.396 bits (690), Expect = 6.685e-92
Identity = 127/195 (65.13%), Postives = 159/195 (81.54%), Query Frame = 3
Query:   60 LWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            +WDWF+G    VL YLGLWKKSG+L+FLGLDNAGKTT+LHMLKDD++A H PTLHPT++EL IG M+FTT+DLGGH QAR++WK+Y  ++D IVFL+DA+D+ R  ES+ EL  L+ D+ +SN P+L+LGNKIDK GA +E+ LR   G+  L TGKG V+R +L G RPLELFMCS+LKRQGYGEGFRWLA Y+
Sbjct:    3 IWDWFTG----VLGYLGLWKKSGKLLFLGLDNAGKTTLLHMLKDDKLAQHVPTLHPTSEELSIGNMRFTTFDLGGHTQARRVWKDYFPAVDAIVFLIDAWDRGRFQESKNELDSLLTDEALSNCPVLILGNKIDKPGAASEDELRNVFGLYQLTTGKGKVARADLPG-RPLELFMCSVLKRQGYGEGFRWLAQYI 192          
BLAST of GTP-binding protein-like protein vs. Ensembl Fly
Match: Sar1 (gene:FBgn0038947 transcript:FBtr0084209)

HSP 1 Score: 270.396 bits (690), Expect = 6.685e-92
Identity = 127/195 (65.13%), Postives = 159/195 (81.54%), Query Frame = 3
Query:   60 LWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            +WDWF+G    VL YLGLWKKSG+L+FLGLDNAGKTT+LHMLKDD++A H PTLHPT++EL IG M+FTT+DLGGH QAR++WK+Y  ++D IVFL+DA+D+ R  ES+ EL  L+ D+ +SN P+L+LGNKIDK GA +E+ LR   G+  L TGKG V+R +L G RPLELFMCS+LKRQGYGEGFRWLA Y+
Sbjct:    3 IWDWFTG----VLGYLGLWKKSGKLLFLGLDNAGKTTLLHMLKDDKLAQHVPTLHPTSEELSIGNMRFTTFDLGGHTQARRVWKDYFPAVDAIVFLIDAWDRGRFQESKNELDSLLTDEALSNCPVLILGNKIDKPGAASEDELRNVFGLYQLTTGKGKVARADLPG-RPLELFMCSVLKRQGYGEGFRWLAQYI 192          
BLAST of GTP-binding protein-like protein vs. Ensembl Fly
Match: Sar1 (gene:FBgn0038947 transcript:FBtr0084206)

HSP 1 Score: 270.396 bits (690), Expect = 6.685e-92
Identity = 127/195 (65.13%), Postives = 159/195 (81.54%), Query Frame = 3
Query:   60 LWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            +WDWF+G    VL YLGLWKKSG+L+FLGLDNAGKTT+LHMLKDD++A H PTLHPT++EL IG M+FTT+DLGGH QAR++WK+Y  ++D IVFL+DA+D+ R  ES+ EL  L+ D+ +SN P+L+LGNKIDK GA +E+ LR   G+  L TGKG V+R +L G RPLELFMCS+LKRQGYGEGFRWLA Y+
Sbjct:    3 IWDWFTG----VLGYLGLWKKSGKLLFLGLDNAGKTTLLHMLKDDKLAQHVPTLHPTSEELSIGNMRFTTFDLGGHTQARRVWKDYFPAVDAIVFLIDAWDRGRFQESKNELDSLLTDEALSNCPVLILGNKIDKPGAASEDELRNVFGLYQLTTGKGKVARADLPG-RPLELFMCSVLKRQGYGEGFRWLAQYI 192          
BLAST of GTP-binding protein-like protein vs. Ensembl Fly
Match: Sar1 (gene:FBgn0038947 transcript:FBtr0084210)

HSP 1 Score: 270.396 bits (690), Expect = 6.685e-92
Identity = 127/195 (65.13%), Postives = 159/195 (81.54%), Query Frame = 3
Query:   60 LWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            +WDWF+G    VL YLGLWKKSG+L+FLGLDNAGKTT+LHMLKDD++A H PTLHPT++EL IG M+FTT+DLGGH QAR++WK+Y  ++D IVFL+DA+D+ R  ES+ EL  L+ D+ +SN P+L+LGNKIDK GA +E+ LR   G+  L TGKG V+R +L G RPLELFMCS+LKRQGYGEGFRWLA Y+
Sbjct:    3 IWDWFTG----VLGYLGLWKKSGKLLFLGLDNAGKTTLLHMLKDDKLAQHVPTLHPTSEELSIGNMRFTTFDLGGHTQARRVWKDYFPAVDAIVFLIDAWDRGRFQESKNELDSLLTDEALSNCPVLILGNKIDKPGAASEDELRNVFGLYQLTTGKGKVARADLPG-RPLELFMCSVLKRQGYGEGFRWLAQYI 192          
BLAST of GTP-binding protein-like protein vs. Ensembl Fly
Match: Arl1 (gene:FBgn0000115 transcript:FBtr0075517)

HSP 1 Score: 108.997 bits (271), Expect = 5.322e-29
Identity = 61/187 (32.62%), Postives = 101/187 (54.01%), Query Frame = 3
Query:   90 GVLSYLG--LWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            GVLSY    L  +  R++ LGLD AGKTT+L+ L+   + T  PT+    +++    +KF  +DLGG    R  W+ Y S+ D I+++VD+ D+ R+  S+ EL ++++++E++   L+VL NK D  G +    +   LG++NL               R  ++F  S  K +G  +   WL+N L
Sbjct:    3 GVLSYFRGLLGSREMRILILGLDGAGKTTILYRLQVGEVVTTIPTIGFNVEQVTYKNLKFQVWDLGGQTSIRPYWRCYYSNTDAIIYVVDSADRDRIGISKDELLYMLREEELAGAILVVLANKQDMDGCMTVAEVHHALGLENL-------------KNRTFQIFKTSATKGEGLDQAMDWLSNTL 176          
BLAST of GTP-binding protein-like protein vs. Ensembl Zebrafish
Match: sar1b (secretion associated, Ras related GTPase 1B [Source:ZFIN;Acc:ZDB-GENE-040426-1958])

HSP 1 Score: 266.159 bits (679), Expect = 4.924e-90
Identity = 126/198 (63.64%), Postives = 155/198 (78.28%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS ++DW     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ I NVP+L+LGNKIDK  A++EE LR   G+    TGKGN+  +EL   RPLE+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFDWIYSGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHAQARRVWKNYLPAINGIVFLVDCIDHGRLAESKTELDALMTDETIGNVPILILGNKIDKPEAISEEKLREIFGLYGQTTGKGNIPLKEL-NTRPLEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Zebrafish
Match: sar1ab (secretion associated, Ras related GTPase 1Ab [Source:ZFIN;Acc:ZDB-GENE-050417-437])

HSP 1 Score: 259.61 bits (662), Expect = 2.384e-87
Identity = 123/198 (62.12%), Postives = 157/198 (79.29%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS ++DW     S VL  LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH QAR++W+NY+ +I+GIV+LVD  D +RL E++ EL  L+ D+ ISNVP+L+LGNKID+  A++E+ LR   G+    TGKGNVS +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFDWLYRGFSNVLQLLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELSIAGMTFTTFDLGGHAQARRVWRNYLPAINGIVYLVDCADHERLQEAKIELDALLTDETISNVPILILGNKIDRPEAISEDALRGMFGLYGHTTGKGNVSLKEL-NLRPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Zebrafish
Match: sar1ab (secretion associated, Ras related GTPase 1Ab [Source:ZFIN;Acc:ZDB-GENE-050417-437])

HSP 1 Score: 259.61 bits (662), Expect = 2.384e-87
Identity = 123/198 (62.12%), Postives = 157/198 (79.29%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS ++DW     S VL  LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH QAR++W+NY+ +I+GIV+LVD  D +RL E++ EL  L+ D+ ISNVP+L+LGNKID+  A++E+ LR   G+    TGKGNVS +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFDWLYRGFSNVLQLLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELSIAGMTFTTFDLGGHAQARRVWRNYLPAINGIVYLVDCADHERLQEAKIELDALLTDETISNVPILILGNKIDRPEAISEDALRGMFGLYGHTTGKGNVSLKEL-NLRPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Zebrafish
Match: arf4a (ADP-ribosylation factor 4a [Source:ZFIN;Acc:ZDB-GENE-040801-109])

HSP 1 Score: 107.071 bits (266), Expect = 4.146e-28
Identity = 65/190 (34.21%), Postives = 103/190 (54.21%), Query Frame = 3
Query:   81 TVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYG--EGFRWLANYL 644
            T+S V S L   KK  R++ +GLD AGKTT+L+ LK   + T  PT+    + +    + FT +D+GG ++ R +W++Y  +  G++F+VD+ D++R  ES +ELQ ++Q+DE+ +  LL+  NK D   A+    L   LG+QNL               R    ++ +    QG G  EG  WL+  L
Sbjct:    4 TISSVFSRL-FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERAQESAEELQKMLQEDELRDAVLLIFANKQDLPNAMPISELTDKLGLQNL---------------RSRTWYVQAACATQGTGLYEGLDWLSEQL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Zebrafish
Match: zgc:77650 (zgc:77650 [Source:ZFIN;Acc:ZDB-GENE-030131-6151])

HSP 1 Score: 105.916 bits (263), Expect = 1.300e-27
Identity = 64/190 (33.68%), Postives = 104/190 (54.74%), Query Frame = 3
Query:   81 TVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYG--EGFRWLANYL 644
            T+S + S L   KK  R++ +GLD AGKTT+L+ LK   + T  PT+    + +    + FT +D+GG ++ R +W++Y  +  G++F+VD+ D++R+ ES +EL  ++Q+DE+ +  LLV  NK D   A+    L   LG+Q+L               R    ++ +    QG G  EG  WL+N L
Sbjct:    4 TISSLFSRL-FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVAESAEELSKMLQEDELRDAVLLVFANKQDLPNAMAVSELTDKLGLQSL---------------RSRTWYVQATCATQGTGLYEGLDWLSNEL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Xenopus
Match: lgals4.2 (lectin, galactoside-binding, soluble, 4, gene 2 [Source:Xenbase;Acc:XB-GENE-5891875])

HSP 1 Score: 265.003 bits (676), Expect = 2.025e-89
Identity = 125/198 (63.13%), Postives = 157/198 (79.29%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+LGNKID+  A++EE LR   G+    TGKGNV  ++L   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCVDHGRLMESKVELNALMTDETISNVPILILGNKIDRPEAISEEKLREIFGLYGQTTGKGNVPLKDLNA-RPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Xenopus
Match: sar1b (secretion associated, Ras related GTPase 1B [Source:NCBI gene;Acc:548588])

HSP 1 Score: 259.225 bits (661), Expect = 4.226e-87
Identity = 123/198 (62.12%), Postives = 155/198 (78.28%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS L+ W S   SGVL +LGL+KK+G+LVFLGLDNAGKTT+L MLKD RM  + PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +I+GIVFL+D  D  RL ES++EL  L+ D+ I+NVP+L+LGNKID+  A++EE L    GI    TGKG V +++L   RPLE+FMCSILKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFLFSWISSGFSGVLQFLGLYKKTGKLVFLGLDNAGKTTLLQMLKDGRMGQYVPTLHPTSEELTIAGMTFTTFDLGGHTQARRVWKNYLPAINGIVFLIDCADHDRLSESKRELDALMADETIANVPILLLGNKIDRPEAISEERLLHLFGIYGQTTGKGKVPQKQL-ANRPLEVFMCSILKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Xenopus
Match: sar1b (secretion associated, Ras related GTPase 1B [Source:NCBI gene;Acc:548588])

HSP 1 Score: 243.817 bits (621), Expect = 5.455e-81
Identity = 115/192 (59.90%), Postives = 146/192 (76.04%), Query Frame = 3
Query:   69 WFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            W        L+ L L+KK+G+LVFLGLDNAGKTT+L MLKD RM  + PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +I+GIVFL+D  D  RL ES++EL  L+ D+ I+NVP+L+LGNKID+  A++EE L    GI    TGKG V +++L   RPLE+FMCSILKRQGYGEGFRWL+ Y+
Sbjct:    7 WVYSIFKSFLAELRLYKKTGKLVFLGLDNAGKTTLLQMLKDGRMGQYVPTLHPTSEELTIAGMTFTTFDLGGHTQARRVWKNYLPAINGIVFLIDCADHDRLSESKRELDALMADETIANVPILLLGNKIDRPEAISEERLLHLFGIYGQTTGKGKVPQKQLAN-RPLEVFMCSILKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Xenopus
Match: arf4 (ADP ribosylation factor 4 [Source:NCBI gene;Acc:595036])

HSP 1 Score: 106.686 bits (265), Expect = 8.832e-28
Identity = 64/190 (33.68%), Postives = 105/190 (55.26%), Query Frame = 3
Query:   81 TVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYG--EGFRWLANYL 644
            T+S + S L   KK  R++ +GLD AGKTT+L+ LK   + T  PT+    + +    + FT +D+GG ++ R +W++Y  +  G++F+VD+ D++R+ E+ +ELQ ++Q+DE+ +  LLV  NK D   A+    +   LG+QNL               R    ++ +    QG G  EG  WL+N L
Sbjct:    4 TISSLFSRL-FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEAAEELQKMLQEDELRDAVLLVFANKQDLPNAMAISEMTDKLGLQNL---------------RNRTWYVQATCATQGTGLYEGLDWLSNEL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Xenopus
Match: arf3 (ADP ribosylation factor 3 [Source:NCBI gene;Acc:100145191])

HSP 1 Score: 99.7525 bits (247), Expect = 3.560e-25
Identity = 57/178 (32.02%), Postives = 97/178 (54.49%), Query Frame = 3
Query:  117 KKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYG--EGFRWLANYL 644
            KK  R++ +GLD AGKTT+L+ LK   + T  PT+    + +    + FT +D+GG ++ R +W++Y  +  G++F+VD+ D++R+ E+++EL  ++ +DE+ +  LLV  NK D   A+N   +   LG+ +L               R    ++ +     G G  EG  WLAN L
Sbjct:   15 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGLHSL---------------RHRNWYIQATCATSGDGLYEGLDWLANQL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Mouse
Match: Sar1a (secretion associated Ras related GTPase 1A [Source:MGI Symbol;Acc:MGI:98230])

HSP 1 Score: 268.855 bits (686), Expect = 4.910e-91
Identity = 126/198 (63.64%), Postives = 158/198 (79.80%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+LGNKID+  A++EE LR   G+    TGKGNV+ +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLMESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNA-RPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Mouse
Match: Sar1a (secretion associated Ras related GTPase 1A [Source:MGI Symbol;Acc:MGI:98230])

HSP 1 Score: 268.855 bits (686), Expect = 4.910e-91
Identity = 126/198 (63.64%), Postives = 158/198 (79.80%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+LGNKID+  A++EE LR   G+    TGKGNV+ +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLMESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNA-RPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Mouse
Match: Sar1b (secretion associated Ras related GTPase 1B [Source:MGI Symbol;Acc:MGI:1913647])

HSP 1 Score: 268.085 bits (684), Expect = 9.153e-91
Identity = 126/198 (63.64%), Postives = 159/198 (80.30%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS ++DW     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +I+GIVFLVD  D +RL ES++EL  L+ D+ I+NVP+L+LGNKID+  A++EE LR   G+    TGKG+VS +EL   RPLE+FMCS+LKRQGYGEGFRW+A Y+
Sbjct:    1 MSFIFDWIYSGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETIANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGSVSLKELNA-RPLEVFMCSVLKRQGYGEGFRWMAQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Mouse
Match: Sar1a (secretion associated Ras related GTPase 1A [Source:MGI Symbol;Acc:MGI:98230])

HSP 1 Score: 185.267 bits (469), Expect = 4.691e-59
Identity = 85/131 (64.89%), Postives = 106/131 (80.92%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLV 443
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLMESKVELNALMTDETISNVPILI 131          
BLAST of GTP-binding protein-like protein vs. Ensembl Mouse
Match: Sar1a (secretion associated Ras related GTPase 1A [Source:MGI Symbol;Acc:MGI:98230])

HSP 1 Score: 146.747 bits (369), Expect = 2.068e-44
Identity = 66/91 (72.53%), Postives = 78/91 (85.71%), Query Frame = 3
Query:  123 SGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKEL 395
            SG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL
Sbjct:    2 SGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLMESKVEL 92          
BLAST of GTP-binding protein-like protein vs. UniProt/SwissProt
Match: sp|Q5R548|SAR1A_PONAB (GTP-binding protein SAR1a OS=Pongo abelii OX=9601 GN=SAR1A PE=2 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 2.078e-90
Identity = 126/198 (63.64%), Postives = 158/198 (79.80%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+LGNKID+  A++EE LR   G+    TGKGNV+ +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNA-RPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. UniProt/SwissProt
Match: sp|Q9NR31|SAR1A_HUMAN (GTP-binding protein SAR1a OS=Homo sapiens OX=9606 GN=SAR1A PE=1 SV=1)

HSP 1 Score: 269.626 bits (688), Expect = 2.078e-90
Identity = 126/198 (63.64%), Postives = 158/198 (79.80%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+LGNKID+  A++EE LR   G+    TGKGNV+ +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNA-RPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. UniProt/SwissProt
Match: sp|Q3T0T7|SAR1B_BOVIN (GTP-binding protein SAR1b OS=Bos taurus OX=9913 GN=SAR1B PE=2 SV=1)

HSP 1 Score: 268.47 bits (685), Expect = 5.145e-90
Identity = 125/198 (63.13%), Postives = 159/198 (80.30%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS ++DW     S VL +LGL+KK+G+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +I+GIVFLVD  D +RL ES++EL  L+ D+ ++NVP+L+LGNKID+  A++EE LR   G+    TGKGNVS +EL   RPLE+FMCS+LKRQGYGEGFRW+A Y+
Sbjct:    1 MSFIFDWIYSGFSSVLQFLGLYKKTGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETVANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGNVSLKELNA-RPLEVFMCSVLKRQGYGEGFRWMAQYI 197          
BLAST of GTP-binding protein-like protein vs. UniProt/SwissProt
Match: sp|Q9CQC9|SAR1B_MOUSE (GTP-binding protein SAR1b OS=Mus musculus OX=10090 GN=Sar1b PE=1 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 6.402e-90
Identity = 126/198 (63.64%), Postives = 159/198 (80.30%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS ++DW     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +I+GIVFLVD  D +RL ES++EL  L+ D+ I+NVP+L+LGNKID+  A++EE LR   G+    TGKG+VS +EL   RPLE+FMCS+LKRQGYGEGFRW+A Y+
Sbjct:    1 MSFIFDWIYSGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAINGIVFLVDCADHERLLESKEELDSLMTDETIANVPILILGNKIDRPEAISEERLREMFGLYGQTTGKGSVSLKELNA-RPLEVFMCSVLKRQGYGEGFRWMAQYI 197          
BLAST of GTP-binding protein-like protein vs. UniProt/SwissProt
Match: sp|Q52NJ3|SAR1A_PIG (GTP-binding protein SAR1a OS=Sus scrofa OX=9823 GN=SAR1A PE=2 SV=1)

HSP 1 Score: 268.085 bits (684), Expect = 7.064e-90
Identity = 126/198 (63.64%), Postives = 158/198 (79.80%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +++W     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGHEQAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ ISNVP+L+LGNKID+  A++EE LR   G+    TGKGNV+ +EL   RP+E+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHPRLMESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNA-RPMEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. TrEMBL
Match: A0A1I8G4X2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig002478g1 PE=3 SV=1)

HSP 1 Score: 301.597 bits (771), Expect = 1.322e-100
Identity = 142/199 (71.36%), Postives = 170/199 (85.43%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNV-SRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MSG WDW SG+VSG+L YLGLWKKSG+LVFLGLDNAGKTT+LHMLKDDRM THNPTL+PTT+EL IGGMKFTT+DLGGHEQARK+WK Y  ++DGIVF+VDA+D+QRL E+  EL  L+ D+++++ P+LVLGNKIDK GA +EE LR+YLG+    TGK +  SR EL  +RPLELFMCSILKRQGYGE FRWL+NY+
Sbjct:    1 MSGWWDWISGSVSGILGYLGLWKKSGKLVFLGLDNAGKTTLLHMLKDDRMGTHNPTLYPTTEELSIGGMKFTTFDLGGHEQARKVWKTYFPAVDGIVFIVDAWDRQRLEEAHVELDNLLADEQVASAPILVLGNKIDKPGAASEEELRYYLGLHGRTTGKSDARSRNEL-AQRPLELFMCSILKRQGYGEAFRWLSNYI 198          
BLAST of GTP-binding protein-like protein vs. TrEMBL
Match: A0A430QPD9 (GTP-binding protein SAR1 OS=Schistosoma bovis OX=6184 GN=DC041_0005783 PE=3 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 4.338e-99
Identity = 134/198 (67.68%), Postives = 171/198 (86.36%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +WDW SG+VS VLSYLGLW+K+G+LVFLGLDNAGKTT+LH LKDDRMA H PTLHPT++EL IGGM+FTT+DLGGHEQAR++WKNY+ ++DG+VF+VDA+D+ R  ES+KEL  L+QD++I++ P+L+LGNKIDK GA +EE LR++LG+Q + TGKG V++ ++   RP+ELFMCSILKRQGYGE F WLA YL
Sbjct:    1 MSFIWDWLSGSVSKVLSYLGLWQKNGKLVFLGLDNAGKTTLLHRLKDDRMAQHVPTLHPTSEELSIGGMRFTTFDLGGHEQARRVWKNYIPAVDGLVFMVDAYDRNRFLESKKELDNLLQDEQIAHAPILILGNKIDKPGAASEEELRYFLGLQGITTGKGTVNKSQIPTGRPIELFMCSILKRQGYGEAFNWLAQYL 198          
BLAST of GTP-binding protein-like protein vs. TrEMBL
Match: A0A183RFT3 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=3 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 4.840e-99
Identity = 133/198 (67.17%), Postives = 171/198 (86.36%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +WDW SG+VS VLSYLGLW+K+G+LVFLGLDNAGKTT+LH LKDDRMA H PTLHPT++EL IGGM+FTT+DLGGHEQAR++WKNY+ ++DG+VF+VDA+D+ R  ES+KEL  L+QD++I++ P+L+LGNKIDK GA +EE LR++LG+Q + TGKG +++ ++   RP+ELFMCSILKRQGYGE F WLA YL
Sbjct:    1 MSFIWDWLSGSVSKVLSYLGLWQKNGKLVFLGLDNAGKTTLLHRLKDDRMAQHVPTLHPTSEELSIGGMRFTTFDLGGHEQARRVWKNYIPAVDGLVFMVDAYDRNRFLESKKELDNLLQDEQIAHAPILILGNKIDKPGAASEEELRYFLGLQGITTGKGTINKSQIPSGRPIELFMCSILKRQGYGEAFNWLAQYL 198          
BLAST of GTP-binding protein-like protein vs. TrEMBL
Match: G4V709 (GTP-binding protein-like protein OS=Schistosoma mansoni OX=6183 GN=Smp_073590 PE=3 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 4.840e-99
Identity = 133/198 (67.17%), Postives = 171/198 (86.36%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +WDW SG+VS VLSYLGLW+K+G+LVFLGLDNAGKTT+LH LKDDRMA H PTLHPT++EL IGGM+FTT+DLGGHEQAR++WKNY+ ++DG+VF+VDA+D+ R  ES+KEL  L+QD++I++ P+L+LGNKIDK GA +EE LR++LG+Q + TGKG +++ ++   RP+ELFMCSILKRQGYGE F WLA YL
Sbjct:    1 MSFIWDWLSGSVSKVLSYLGLWQKNGKLVFLGLDNAGKTTLLHRLKDDRMAQHVPTLHPTSEELSIGGMRFTTFDLGGHEQARRVWKNYIPAVDGLVFMVDAYDRNRFLESKKELDNLLQDEQIAHAPILILGNKIDKPGAASEEELRYFLGLQGITTGKGTINKSQIPSGRPIELFMCSILKRQGYGEAFNWLAQYL 198          
BLAST of GTP-binding protein-like protein vs. TrEMBL
Match: A0A183JBK6 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS61 PE=3 SV=1)

HSP 1 Score: 296.975 bits (759), Expect = 7.831e-99
Identity = 133/198 (67.17%), Postives = 171/198 (86.36%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS +WDW SG+VS VLSYLGLW+K+G+LVFLGLDNAGKTT+LH LKDDRMA H PTLHPT++EL IGGM+FTT+DLGGHEQAR++WKNY+ ++DG+VF+VDA+D+ R  ES+KEL  L+QD++I++ P+L+LGNKIDK GA +EE LR++LG+Q + TGKG +++ ++   RP+ELFMCSILKRQGYGE F WLA YL
Sbjct:    1 MSFIWDWLSGSVSKVLSYLGLWQKNGKLVFLGLDNAGKTTLLHRLKDDRMAQHVPTLHPTSEELSIGGMRFTTFDLGGHEQARRVWKNYIPAVDGLVFMVDAYDRNRFLESKKELDNLLQDEQIAHAPILILGNKIDKPGAASEEELRYFLGLQGITTGKGTINKSQIPTGRPIELFMCSILKRQGYGEAFNWLAQYL 198          
BLAST of GTP-binding protein-like protein vs. Ensembl Cavefish
Match: ENSAMXT00000056144.1 (pep primary_assembly:Astyanax_mexicanus-2.0:6:22046095:22052022:-1 gene:ENSAMXG00000034192.1 transcript:ENSAMXT00000056144.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 262.692 bits (670), Expect = 2.473e-88
Identity = 123/198 (62.12%), Postives = 157/198 (79.29%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS ++DW     SGVL +LGL+KK+G+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +++G+VFLVD  D +RL ES+ EL  L+ D+ ISNVP+LVLGNKID+  A++E  LR    +    TGKG+VS +EL   RPLE+FMCS+LK+QGYGEGFRWL+ Y+
Sbjct:   21 MSFIFDWIYRGFSGVLQFLGLYKKTGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAVNGVVFLVDCADHERLAESKVELDALLSDETISNVPILVLGNKIDRPEAVSEVRLREVFALDGQTTGKGSVSLKEL-NVRPLEVFMCSVLKKQGYGEGFRWLSQYI 217          
BLAST of GTP-binding protein-like protein vs. Ensembl Cavefish
Match: sar1b (secretion associated, Ras related GTPase 1B [Source:ZFIN;Acc:ZDB-GENE-040426-1958])

HSP 1 Score: 169.859 bits (429), Expect = 5.765e-53
Identity = 80/122 (65.57%), Postives = 97/122 (79.51%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKEL---QFLI 407
            MS ++DW     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL   QFL+
Sbjct:    1 MSFIFDWIYSGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHAQARRVWKNYLPAINGIVFLVDCIDHPRLAESKTELDVNQFLL 122          
BLAST of GTP-binding protein-like protein vs. Ensembl Cavefish
Match: ENSAMXT00000055305.1 (pep primary_assembly:Astyanax_mexicanus-2.0:6:22048234:22051962:-1 gene:ENSAMXG00000034192.1 transcript:ENSAMXT00000055305.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 164.851 bits (416), Expect = 4.603e-51
Identity = 74/114 (64.91%), Postives = 94/114 (82.46%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKE 392
            MS ++DW     SGVL +LGL+KK+G+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +++G+VFLVD  D +RL ES+ E
Sbjct:    1 MSFIFDWIYRGFSGVLQFLGLYKKTGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHVQARRVWKNYLPAVNGVVFLVDCADHERLAESKVE 114          
BLAST of GTP-binding protein-like protein vs. Ensembl Cavefish
Match: tysnd1 (trypsin domain containing 1 [Source:NCBI gene;Acc:103024644])

HSP 1 Score: 157.532 bits (397), Expect = 1.999e-48
Identity = 73/119 (61.34%), Postives = 91/119 (76.47%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLI 407
            MS L DWF    SGVL  LGL+KKSG+LV LGLDNAGKTT+L MLKDDR+    PTLHPT++EL I GM FTT+DLGGH QAR++WKNY+ +I+GIV++VD  D +RL E++ E    +
Sbjct:    1 MSFLLDWFYKGFSGVLQLLGLYKKSGKLVLLGLDNAGKTTLLQMLKDDRLGQPVPTLHPTSEELTIAGMTFTTFDLGGHAQARRVWKNYLPAINGIVYIVDCADHERLLEAKNEFNCFL 119          
BLAST of GTP-binding protein-like protein vs. Ensembl Cavefish
Match: ENSAMXT00000046201.1 (ADP-ribosylation factor 4-like [Source:NCBI gene;Acc:107197496])

HSP 1 Score: 104.375 bits (259), Expect = 3.958e-27
Identity = 60/178 (33.71%), Postives = 98/178 (55.06%), Query Frame = 3
Query:  117 KKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYG--EGFRWLANYL 644
            KK  R++ +GLD AGKTT+L+ LK   + T  PT+    + +    + FT +D+GG ++ R +W++Y  +  G++F+VD+ D++R+ ES +EL  ++Q+DE+ +  LLV  NK D   A+    L   LG+Q+L               R    ++ +    QG G  EG  WL+N L
Sbjct:   15 KKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVAESAEELAKMLQEDELRDAVLLVFANKQDLPNAMAVSELTDKLGLQSL---------------RSRTWYVQATCATQGTGLYEGLDWLSNEL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Sea Lamprey
Match: sar1b (secretion associated, Ras related GTPase 1B [Source:ZFIN;Acc:ZDB-GENE-040426-1958])

HSP 1 Score: 251.906 bits (642), Expect = 3.947e-85
Identity = 121/201 (60.20%), Postives = 154/201 (76.62%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQ---ARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS  + W     S +L +LGL+KKSG+LVFLGLDNAGKTT+L MLKDDRM    PTLHPT++EL + GM FTT+DLGGH Q   AR+IWKNY+ +IDG+VFLVD  D +RL E++KEL  LI D+ I+NVP+L+LGNKID+  A++EE LR   G+    TGKGN+  ++L   RPLE+FMCS+LKRQGYGEGFRW++ Y+
Sbjct:    1 MSFFFSWIYKGFSSMLQFLGLYKKSGKLVFLGLDNAGKTTLLCMLKDDRMGQPVPTLHPTSEELTMAGMTFTTFDLGGHAQGSSARRIWKNYLPAIDGVVFLVDCTDHERLTEAKKELDALITDETIANVPVLILGNKIDRPEAISEERLREVFGLYGQTTGKGNIPLKDL-NTRPLEVFMCSVLKRQGYGEGFRWMSQYI 200          
BLAST of GTP-binding protein-like protein vs. Ensembl Sea Lamprey
Match: arf4a (ADP-ribosylation factor 4a [Source:ZFIN;Acc:ZDB-GENE-040801-109])

HSP 1 Score: 103.99 bits (258), Expect = 1.119e-27
Identity = 65/190 (34.21%), Postives = 104/190 (54.74%), Query Frame = 3
Query:   81 TVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYG--EGFRWLANYL 644
             +S VL+ L L  K+ R++ +GLD AGKTT+L+ LK   + T  PT     + +    + FT +D+GG ++ R +W++Y  +  G++F+VD+ D++R+ E+ +ELQ ++Q+D++ +  LLVL NK D   A+    L   LG               L G R    F+ +    QG G  EG  WL+N L
Sbjct:    4 AISRVLAAL-LGTKAMRILMVGLDAAGKTTILYKLKLGEIVTTIPTTRFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEAAEELQKMMQEDDLKDAALLVLANKQDLPNAMAIGELTEKLG---------------LHGLRNRAWFVQATCAPQGTGLCEGLDWLSNKL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Sea Lamprey
Match: arf4a (ADP-ribosylation factor 4a [Source:ZFIN;Acc:ZDB-GENE-040801-109])

HSP 1 Score: 103.99 bits (258), Expect = 1.299e-27
Identity = 65/190 (34.21%), Postives = 104/190 (54.74%), Query Frame = 3
Query:   81 TVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYG--EGFRWLANYL 644
             +S VL+ L L  K+ R++ +GLD AGKTT+L+ LK   + T  PT     + +    + FT +D+GG ++ R +W++Y  +  G++F+VD+ D++R+ E+ +ELQ ++Q+D++ +  LLVL NK D   A+    L   LG               L G R    F+ +    QG G  EG  WL+N L
Sbjct:    4 AISRVLAAL-LGTKAMRILMVGLDAAGKTTILYKLKLGEIVTTIPTTRFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERIQEAAEELQKMMQEDDLKDAALLVLANKQDLPNAMAIGELTEKLG---------------LHGLRNRAWFVQATCAPQGTGLCEGLDWLSNKL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Sea Lamprey
Match: arl2 (ADP-ribosylation factor-like 2 [Source:ZFIN;Acc:ZDB-GENE-041010-19])

HSP 1 Score: 103.219 bits (256), Expect = 2.399e-27
Identity = 53/132 (40.15%), Postives = 81/132 (61.36%), Query Frame = 3
Query:  129 RLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLIT 524
            RL+ LGLDNAGKTT+L     + + T +PTL      L   G K   +D+GG +  R  W+NY  S DG+V++VD+ D++RL +  KEL  L+Q++ ++   LLV  NK D  GAL+ +N++  L ++ + T
Sbjct:   18 RLLMLGLDNAGKTTILKKFNGEDIDTISPTLGFNIKTLEHRGFKLNIWDVGGQKSLRSYWRNYFESTDGLVWVVDSADRRRLEDCGKELASLLQEERLTGATLLVFANKQDLAGALSADNIKEALDLEAIKT 149          
BLAST of GTP-binding protein-like protein vs. Ensembl Sea Lamprey
Match: ARF6 (ADP ribosylation factor 6 [Source:HGNC Symbol;Acc:HGNC:659])

HSP 1 Score: 100.523 bits (249), Expect = 2.144e-26
Identity = 63/173 (36.42%), Postives = 97/173 (56.07%), Query Frame = 3
Query:  129 RLVFLGLDNAGKTTMLHMLKDDR-MATHN--PTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLAN 638
            R++ LGLDNAGKTT+L+ LK  + +AT +  PT+    + +      F+ +D+GG  + R +W++Y +    IVF+VD  D+ R+ E+++EL  L+ D E+  V LLVL NK D   A+    L+  LG+     G+G+        RR L    C+I+   G  EG  WLA+
Sbjct:   15 RILMLGLDNAGKTTILYKLKSGKGVATKSTVPTVGFNVETVSYKSASFSVWDVGGQHKIRSLWRHYYTGTQAIVFVVDCGDRDRVDEARQELSSLLSDSEMRGVSLLVLANKQDAPEAMRPAELQEALGLPR--PGEGD-------SRRWLVQPSCAIMG-HGLYEGLVWLAD 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Yeast
Match: SAR1 (ARF family GTPase; component of the COPII vesicle coat; required for transport vesicle formation during ER to Golgi protein transport; lowers membrane rigidity aiding vesicle formation; localizes to ER-mitochondrial contact sites where it enhances membrane curvature, thereby reducing contact size via its N-terminal amphipathic helix; regulates mitochondrial fission and fusion dynamics [Source:SGD;Acc:S000006139])

HSP 1 Score: 214.542 bits (545), Expect = 9.883e-71
Identity = 106/198 (53.54%), Postives = 141/198 (71.21%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            M+G WD F G    VL+ LGLW K G+L+FLGLDNAGKTT+LHMLK+DR+AT  PT HPT++EL IG +KFTT+DLGGH QAR++WK+Y   ++GIVFLVDA D +R  E++ EL  L    E+ +VP ++LGNKID   A++E  LR  LG+ N          Q + G+RP+E+FMCS++ R GY E F+WL+ Y+
Sbjct:    1 MAG-WDIF-GWFRDVLASLGLWNKHGKLLFLGLDNAGKTTLLHMLKNDRLATLQPTWHPTSEELAIGNIKFTTFDLGGHIQARRLWKDYFPEVNGIVFLVDAADPERFDEARVELDALFNIAELKDVPFVILGNKIDAPNAVSEAELRSALGLLN------TTGSQRIEGQRPVEVFMCSVVMRNGYLEAFQWLSQYI 190          
BLAST of GTP-binding protein-like protein vs. Ensembl Yeast
Match: ARL1 (Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; role in membrane organization at trans-Golgi network; required for delivery of Atg9p to the phagophore assembly site during autophagy under high temperature stress; required with Ypt6p for starvation-induced autophagy; required for the CVT pathway under non-starvation conditions; G protein of the Ras superfamily, similar to ADP-ribosylation factor [Source:SGD;Acc:S000000368])

HSP 1 Score: 100.908 bits (250), Expect = 1.338e-26
Identity = 58/180 (32.22%), Postives = 92/180 (51.11%), Query Frame = 3
Query:  111 LW--KKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            LW   K  R++ LGLD AGKTT+L+ L+   + T  PT+    + L    +K   +DLGG    R  W+ Y +    ++F+VD+ D+ R+  + KEL  ++Q++E+ +  LLV  NK D+ GAL+   +   L   NL+  K           R   +   S +K +G  EG  WL + +
Sbjct:   12 LWGSNKELRILILGLDGAGKTTILYRLQIGEVVTTKPTIGFNVETLSYKNLKLNVWDLGGQTSIRPYWRCYYADTAAVIFVVDSTDKDRMSTASKELHLMLQEEELQDAALLVFANKQDQPGALSASEVSKEL---NLVELKD----------RSWSIVASSAIKGEGITEGLDWLIDVI 178          
BLAST of GTP-binding protein-like protein vs. Ensembl Yeast
Match: ARF3 (Glucose-repressible ADP-ribosylation factor; GTPase of Ras superfamily involved in regulating cell polarity and invasive growth; localizes to dynamic spots at plasma membrane and modulates PtdIns(4,5)P2 levels to facilitate endocytosis; required for localization of endocytic protein Lsb5p to correct cortical site in cells; also has mRNA binding activity; homolog of mammalian Arf6 [Source:SGD;Acc:S000005620])

HSP 1 Score: 97.4413 bits (241), Expect = 2.059e-25
Identity = 52/173 (30.06%), Postives = 96/173 (55.49%), Query Frame = 3
Query:  120 KSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLAN 638
            K  +++ LGLD AGKTT+L+ LK +++ T  PT+    + +    +KF  +D+GG ++ R +W++Y  +   ++F++D+  + R+ E+++EL  +I + E+ NV LLV  NK D   A+  + +  +L ++       N+  Q      P  +   + L  QG  EG  W++N
Sbjct:   16 KEMKILMLGLDKAGKTTILYKLKLNKIKTSTPTVGFNVETVTYKNVKFNMWDVGGQQRLRPLWRHYFPATTALIFVIDSSARNRMEEAKEELYSIIGEKEMENVVLLVWANKQDLKDAMKPQEVSDFLELEK------NLKNQ------PWCVIGSNALSGQGLVEGLSWISN 176          
BLAST of GTP-binding protein-like protein vs. Ensembl Yeast
Match: ARF1 (ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6 [Source:SGD;Acc:S000002351])

HSP 1 Score: 97.4413 bits (241), Expect = 2.548e-25
Identity = 52/175 (29.71%), Postives = 94/175 (53.71%), Query Frame = 3
Query:  120 KSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            K  R++ +GLD AGKTT+L+ LK   + T  PT+    + +    + FT +D+GG ++ R +W++Y  + +G++F+VD+ D+ R+ E+++ +Q ++ +DE+ N   LV  NK D   A++   +   LG+ ++               RP  +        +G  EG  WL+N L
Sbjct:   16 KEMRILMVGLDGAGKTTVLYKLKLGEVITTIPTIGFNVETVQYKNISFTVWDVGGQDRIRSLWRHYYRNTEGVIFVVDSNDRSRIGEAREVMQRMLNEDELRNAAWLVFANKQDLPEAMSAAEITEKLGLHSI-------------RNRPWFIQATCATSGEGLYEGLEWLSNSL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Yeast
Match: ARF2 (ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6 [Source:SGD;Acc:S000002296])

HSP 1 Score: 95.5153 bits (236), Expect = 1.350e-24
Identity = 51/175 (29.14%), Postives = 94/175 (53.71%), Query Frame = 3
Query:  120 KSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            K  R++ +GLD AGKTT+L+ LK   + T  PT+    + +    + FT +D+GG ++ R +W++Y  + +G++F++D+ D+ R+ E+++ +Q ++ +DE+ N   LV  NK D   A++   +   LG+ ++               RP  +        +G  EG  WL+N L
Sbjct:   16 KEMRILMVGLDGAGKTTVLYKLKLGEVITTIPTIGFNVETVQYKNISFTVWDVGGQDRIRSLWRHYYRNTEGVIFVIDSNDRSRIGEAREVMQRMLNEDELRNAVWLVFANKQDLPEAMSAAEITEKLGLHSI-------------RNRPWFIQSTCATSGEGLYEGLEWLSNNL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Nematostella
Match: EDO45717 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RS05])

HSP 1 Score: 258.07 bits (658), Expect = 1.938e-87
Identity = 120/195 (61.54%), Postives = 159/195 (81.54%), Query Frame = 3
Query:   60 LWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            LWDWF+G     L YLGL++KSG+LVFLGLDNAGKTT+LHMLKDDRMA H PTLHPT++EL +GGM+FTT+DLGGH QAR+IWK+Y  +++GIVF++D  D +RL ES+KEL  L+ D+++S+ P+L+LGNKID  GA+ E+ +R   G+    TGKG+V+ ++L   RP+ELFMCS+LKR+GYGEGFRWL+ Y+
Sbjct:    3 LWDWFTG----ALGYLGLYQKSGKLVFLGLDNAGKTTLLHMLKDDRMAQHVPTLHPTSEELSMGGMRFTTFDLGGHRQARRIWKDYFPAVNGIVFIIDCADFERLAESKKELDSLLADEQLSSCPVLILGNKIDIPGAVGEDYIRQNFGLFGQTTGKGSVAAKDL-ATRPMELFMCSVLKREGYGEGFRWLSEYI 192          
BLAST of GTP-binding protein-like protein vs. Ensembl Nematostella
Match: EDO48392 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJK4])

HSP 1 Score: 105.916 bits (263), Expect = 4.577e-28
Identity = 57/176 (32.39%), Postives = 96/176 (54.55%), Query Frame = 3
Query:  117 KKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            K+  R++ +GLDNAGKTT+L+ LK + + +  PTL    + +    + FT +D+GG ++ R +W+ Y     GI+F+VD+ D++R  E++ EL  L+ ++E+  V LLV+ NK D   A+    +R  L +             E+ GR       C++ K +G  EG  W+A  +
Sbjct:   15 KEEVRILMIGLDNAGKTTILYRLKLEEVVSTVPTLGFNVETVTYKNISFTVWDIGGQDKIRALWRVYYQGCQGIIFVVDSADRERAEEARNELHKLLAEEELQQVILLVIANKQDMANAMTASEIREKLKL------------NEIRGRPWFVQSACAV-KGEGLFEGLDWMATQI 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Nematostella
Match: EDO48359 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJD9])

HSP 1 Score: 105.145 bits (261), Expect = 8.479e-28
Identity = 60/190 (31.58%), Postives = 108/190 (56.84%), Query Frame = 3
Query:   81 TVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYG--EGFRWLANYL 644
            T+S + + L   KK  R++ +GLD AGKTT+L+ LK   + T  PT+    + +    + FT +D+GG ++ R +W++Y  +  G++++VD+ D++R+ ES++EL  ++Q+DE+ +  +LV+ NK D   AL+   +   LG+Q++               R  + ++ +    QG G  EG  WL+N L
Sbjct:    4 TISSLFTRL-FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVESVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIYVVDSNDRERVNESKEELNKMLQEDELKDAVVLVMANKQDLPNALSVSEITEKLGLQSI---------------RDRQWYIQAACATQGTGLYEGLDWLSNEL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Nematostella
Match: EDO48360 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJE1])

HSP 1 Score: 105.145 bits (261), Expect = 1.457e-27
Identity = 60/190 (31.58%), Postives = 108/190 (56.84%), Query Frame = 3
Query:   81 TVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYG--EGFRWLANYL 644
            T+S + + L   KK  R++ +GLD AGKTT+L+ LK   + T  PT+    + +    + FT +D+GG ++ R +W++Y  +  G++++VD+ D++R+ ES++EL  ++Q+DE+ +  +LV+ NK D   AL+   +   LG+Q++               R  + ++ +    QG G  EG  WL+N L
Sbjct:    4 TISSLFTRL-FGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVESVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIYVVDSNDRERVNESKEELNKMLQEDELKDAVVLVMANKQDLPNALSVSEITEKLGLQSI---------------RDRQWYIQAACATQGTGLYEGLDWLSNEL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Nematostella
Match: EDO42683 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0H8])

HSP 1 Score: 101.293 bits (251), Expect = 1.905e-26
Identity = 54/175 (30.86%), Postives = 91/175 (52.00%), Query Frame = 3
Query:  120 KSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            K  R++ LGLD AGKTT+L+ L+   + T  PT+    + +    +KF  +DLGG    R  W+ Y ++ D ++++VD+ D+ R+  S++EL  ++++DE+    L+V  NK D  GA++   +   LG+  L               R   +F  S +K +G  E   WL + L
Sbjct:   16 KELRILILGLDGAGKTTILYRLQVGEVVTTIPTIGFNVETVTYKNLKFQVWDLGGQTSIRPYWRCYYANTDAVIYVVDSVDKDRIGISKQELLAMLEEDELKKAILIVFANKQDMEGAMSSSEVSNALGLSAL-------------KSRTWAIFKTSAVKGEGLDEAMEWLVDAL 177          
BLAST of GTP-binding protein-like protein vs. Ensembl Medaka
Match: ENSORLT00000030237.1 (secretion associated Ras related GTPase 1A [Source:NCBI gene;Acc:101158641])

HSP 1 Score: 263.848 bits (673), Expect = 7.490e-89
Identity = 123/198 (62.12%), Postives = 157/198 (79.29%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS ++DW     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL IGGM FTT+DLGGH QAR++WKNY+ +++G+VFLVD  D  RLPES+ EL  L+ D+ I +VP+LVLGNKID+  A++E  LR    +   +TGKGN+ R++L   RPLE+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:   23 MSFIFDWIYRGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIGGMTFTTFDLGGHVQARRVWKNYLPAVNGVVFLVDCADHDRLPESKVELDALLGDETIEDVPVLVLGNKIDRPEAISEGGLRGAFALDGQVTGKGNIPRKDLNA-RPLEVFMCSVLKRQGYGEGFRWLSQYI 219          
BLAST of GTP-binding protein-like protein vs. Ensembl Medaka
Match: sar1b (secretion associated Ras related GTPase 1B [Source:NCBI gene;Acc:101172263])

HSP 1 Score: 262.307 bits (669), Expect = 1.720e-88
Identity = 124/198 (62.63%), Postives = 156/198 (78.79%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS L+DW     + VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL I GM FTT+DLGGH+QAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ I NVP+L+LGNKID+  A++EE LR   G+    TGKGN+  ++L   RPLE+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFLFDWIYSGFNSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHQQARRVWKNYLPAINGIVFLVDCADPDRLTESKTELDALMTDETIGNVPILILGNKIDRQDAISEERLRERFGLYGHTTGKGNIPLKDL-NTRPLEVFMCSVLKRQGYGEGFRWLSQYI 197          
BLAST of GTP-binding protein-like protein vs. Ensembl Medaka
Match: sar1ab (GTP-binding protein SAR1b [Source:NCBI gene;Acc:101155138])

HSP 1 Score: 261.151 bits (666), Expect = 1.191e-86
Identity = 123/200 (61.50%), Postives = 157/200 (78.50%), Query Frame = 3
Query:   45 LKMSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            + MS LWDW     S VL  LGL+KKSG+LVFLGLDNAGKTT+L ML+DDR+  HNPTL+PT++EL I GM FTT+DLGGH QAR+IWKNY  +I+GIV+LVD  D +RL E++ EL  L+ D+ I+N+P+L+LGNKID+  A++E+ LR   G+    TGKGNVS +EL   RPLE+FMCS+LKRQGYG+GFRWL+ Y+
Sbjct:   96 VPMSFLWDWIYRGFSSVLHLLGLYKKSGKLVFLGLDNAGKTTLLQMLRDDRLGQHNPTLYPTSEELTIAGMTFTTFDLGGHTQARRIWKNYFPAINGIVYLVDCADHERLGEAKTELDALLTDETIANIPVLILGNKIDRPEAISEDGLRGLFGLHGHTTGKGNVSLKEL-NLRPLEVFMCSVLKRQGYGDGFRWLSQYI 294          
BLAST of GTP-binding protein-like protein vs. Ensembl Medaka
Match: ENSORLT00000032693.1 (secretion associated Ras related GTPase 1A [Source:NCBI gene;Acc:101158641])

HSP 1 Score: 250.366 bits (638), Expect = 3.708e-83
Identity = 123/224 (54.91%), Postives = 157/224 (70.09%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGK--------------------------GNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS ++DW     S VL +LGL+KKSG+LVFLGLDNAGKTT+LHMLKDDR+  H PTLHPT++EL IGGM FTT+DLGGH QAR++WKNY+ +++G+VFLVD  D  RLPES+ EL  L+ D+ I +VP+LVLGNKID+  A++E  LR    +   +TGK                          GN+ R++L   RPLE+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:   23 MSFIFDWIYRGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIGGMTFTTFDLGGHVQARRVWKNYLPAVNGVVFLVDCADHDRLPESKVELDALLGDETIEDVPVLVLGNKIDRPEAISEGGLRGAFALDGQVTGKVSMSGSQTYGRIRSFFLLKSSLFYQQGNIPRKDLNA-RPLEVFMCSVLKRQGYGEGFRWLSQYI 245          
BLAST of GTP-binding protein-like protein vs. Ensembl Medaka
Match: sar1b (secretion associated Ras related GTPase 1B [Source:NCBI gene;Acc:101172263])

HSP 1 Score: 228.024 bits (580), Expect = 4.145e-75
Identity = 113/198 (57.07%), Postives = 148/198 (74.75%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MS L+DW     + VL +LGL+KKSG+LVFLGLDNAGKTT+LH+     +   + T  P+++EL I GM FTT+DLGGH+QAR++WKNY+ +I+GIVFLVD  D  RL ES+ EL  L+ D+ I NVP+L+LGNKID+  A++EE LR   G+    TGKGN+  ++L   RPLE+FMCS+LKRQGYGEGFRWL+ Y+
Sbjct:    1 MSFLFDWIYSGFNSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHI----ELVLLSLT-RPSSEELTIAGMTFTTFDLGGHQQARRVWKNYLPAINGIVFLVDCADPDRLTESKTELDALMTDETIGNVPILILGNKIDRQDAISEERLRERFGLYGHTTGKGNIPLKDL-NTRPLEVFMCSVLKRQGYGEGFRWLSQYI 192          
BLAST of GTP-binding protein-like protein vs. Planmine SMEST
Match: SMESG000018556.1 (SMESG000018556.1)

HSP 1 Score: 405.601 bits (1041), Expect = 3.052e-145
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 3
Query:   51 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 644
            MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL
Sbjct:    1 MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFYLGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL 198          
BLAST of GTP-binding protein-like protein vs. Planmine SMEST
Match: SMESG000052759.1 (SMESG000052759.1)

HSP 1 Score: 106.301 bits (264), Expect = 7.242e-28
Identity = 57/163 (34.97%), Postives = 96/163 (58.90%), Query Frame = 3
Query:  129 RLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIG-GMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENL-------------RFYLGIQNLITGKGNVSRQELGGRRPLE 575
            R++ LGLD AGKTT+L+ LK +   T  PT+    +E+ IG G+KFT +D+GG E+ R +WK+Y ++  G++++VD+ D +RL ES+ EL  + + DE+ NVP++V+ NK D   A++ + +             ++Y+     ++G G     E  G+   E
Sbjct:   22 RILMLGLDAAGKTTILYKLKLNETVTTIPTIGFNVEEVSIGKGLKFTIWDVGGQEKIRLLWKHYYTNCHGLIYVVDSNDAERLKESRDELFAIAECDEMRNVPIVVIANKQDLPNAMSPDRIVEGLELSKLKPTHKWYIQACCAVSGDGLYEALEAAGKLSAE 184          
BLAST of GTP-binding protein-like protein vs. Planmine SMEST
Match: SMESG000052764.1 (SMESG000052764.1)

HSP 1 Score: 106.301 bits (264), Expect = 7.555e-28
Identity = 57/163 (34.97%), Postives = 96/163 (58.90%), Query Frame = 3
Query:  129 RLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIG-GMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENL-------------RFYLGIQNLITGKGNVSRQELGGRRPLE 575
            R++ LGLD AGKTT+L+ LK +   T  PT+    +E+ IG G+KFT +D+GG E+ R +WK+Y ++  G++++VD+ D +RL ES+ EL  + + DE+ NVP++V+ NK D   A++ + +             ++Y+     ++G G     E  G+   E
Sbjct:   22 RILMLGLDAAGKTTILYKLKLNETVTTIPTIGFNVEEVSIGKGLKFTIWDVGGQEKIRLLWKHYYTNCHGLIYVVDSNDAERLKESRDELFAIAECDEMRNVPIVVIANKQDLPNAMSPDRIVEGLELSKLKPTHKWYIQACCAVSGDGLYEALEAAGKLSAE 184          
BLAST of GTP-binding protein-like protein vs. Planmine SMEST
Match: SMESG000052741.1 (SMESG000052741.1)

HSP 1 Score: 105.145 bits (261), Expect = 2.362e-27
Identity = 51/118 (43.22%), Postives = 82/118 (69.49%), Query Frame = 3
Query:  129 RLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIG-GMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALN 479
            R++ LGLD AGKTT+L+ LK +   T  PT+    +E+ IG G+KFT +D+GG E+ R +WK+Y ++  G++++VD+ D +RL ES+ EL  + + DE+ NVP++V+ NK D   A++
Sbjct:   22 RILMLGLDAAGKTTILYKLKLNETVTTIPTIGFNVEEVSIGKGLKFTIWDVGGQEKIRLLWKHYYTNCHGLIYVVDSNDAERLKESRDELFAIAECDEMRNVPIVVIANKQDLPNAMS 139          
BLAST of GTP-binding protein-like protein vs. Planmine SMEST
Match: SMESG000052739.1 (SMESG000052739.1)

HSP 1 Score: 104.76 bits (260), Expect = 2.570e-27
Identity = 51/118 (43.22%), Postives = 82/118 (69.49%), Query Frame = 3
Query:  129 RLVFLGLDNAGKTTMLHMLKDDRMATHNPTLHPTTDELIIG-GMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLVDAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALN 479
            R++ LGLD AGKTT+L+ LK +   T  PT+    +E+ IG G+KFT +D+GG E+ R +WK+Y ++  G++++VD+ D +RL ES+ EL  + + DE+ NVP++V+ NK D   A++
Sbjct:   22 RILMLGLDAAGKTTILYKLKLNETVTTIPTIGFNVEEVSIGKGLKFTIWDVGGQEKIRLLWKHYYTNCHGLIYVVDSNDAERLKESRDELFAIAECDEMRNVPIVVIANKQDLPNAMS 139          
The following BLAST results are available for this feature:
BLAST of GTP-binding protein-like protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SAR1A4.327e-9163.64secretion associated Ras related GTPase 1A [Source... [more]
SAR1A4.327e-9163.64secretion associated Ras related GTPase 1A [Source... [more]
SAR1A4.327e-9163.64secretion associated Ras related GTPase 1A [Source... [more]
SAR1A4.327e-9163.64secretion associated Ras related GTPase 1A [Source... [more]
SAR1B6.087e-9062.63secretion associated Ras related GTPase 1B [Source... [more]
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BLAST of GTP-binding protein-like protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sar-11.646e-9061.62GTP-binding protein SAR1 [Source:UniProtKB/Swiss-... [more]
arl-13.717e-2834.22ADP-ribosylation factor-like protein 1 [Source:Un... [more]
arl-13.717e-2834.22ADP-ribosylation factor-like protein 1 [Source:Un... [more]
arf-34.176e-2832.58ADP-Ribosylation Factor related; ADP-ribosylation ... [more]
arl-51.535e-2630.29ADP-ribosylation factor-like protein 5 [Source:Un... [more]
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BLAST of GTP-binding protein-like protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Sar16.685e-9265.13gene:FBgn0038947 transcript:FBtr0084208[more]
Sar16.685e-9265.13gene:FBgn0038947 transcript:FBtr0084209[more]
Sar16.685e-9265.13gene:FBgn0038947 transcript:FBtr0084206[more]
Sar16.685e-9265.13gene:FBgn0038947 transcript:FBtr0084210[more]
Arl15.322e-2932.62gene:FBgn0000115 transcript:FBtr0075517[more]
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BLAST of GTP-binding protein-like protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sar1b4.924e-9063.64secretion associated, Ras related GTPase 1B [Sourc... [more]
sar1ab2.384e-8762.12secretion associated, Ras related GTPase 1Ab [Sour... [more]
sar1ab2.384e-8762.12secretion associated, Ras related GTPase 1Ab [Sour... [more]
arf4a4.146e-2834.21ADP-ribosylation factor 4a [Source:ZFIN;Acc:ZDB-GE... [more]
zgc:776501.300e-2733.68zgc:77650 [Source:ZFIN;Acc:ZDB-GENE-030131-6151][more]
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BLAST of GTP-binding protein-like protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lgals4.22.025e-8963.13lectin, galactoside-binding, soluble, 4, gene 2 [S... [more]
sar1b4.226e-8762.12secretion associated, Ras related GTPase 1B [Sourc... [more]
sar1b5.455e-8159.90secretion associated, Ras related GTPase 1B [Sourc... [more]
arf48.832e-2833.68ADP ribosylation factor 4 [Source:NCBI gene;Acc:59... [more]
arf33.560e-2532.02ADP ribosylation factor 3 [Source:NCBI gene;Acc:10... [more]
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BLAST of GTP-binding protein-like protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Sar1a4.910e-9163.64secretion associated Ras related GTPase 1A [Source... [more]
Sar1a4.910e-9163.64secretion associated Ras related GTPase 1A [Source... [more]
Sar1b9.153e-9163.64secretion associated Ras related GTPase 1B [Source... [more]
Sar1a4.691e-5964.89secretion associated Ras related GTPase 1A [Source... [more]
Sar1a2.068e-4472.53secretion associated Ras related GTPase 1A [Source... [more]
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BLAST of GTP-binding protein-like protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5R548|SAR1A_PONAB2.078e-9063.64GTP-binding protein SAR1a OS=Pongo abelii OX=9601 ... [more]
sp|Q9NR31|SAR1A_HUMAN2.078e-9063.64GTP-binding protein SAR1a OS=Homo sapiens OX=9606 ... [more]
sp|Q3T0T7|SAR1B_BOVIN5.145e-9063.13GTP-binding protein SAR1b OS=Bos taurus OX=9913 GN... [more]
sp|Q9CQC9|SAR1B_MOUSE6.402e-9063.64GTP-binding protein SAR1b OS=Mus musculus OX=10090... [more]
sp|Q52NJ3|SAR1A_PIG7.064e-9063.64GTP-binding protein SAR1a OS=Sus scrofa OX=9823 GN... [more]
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BLAST of GTP-binding protein-like protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8G4X21.322e-10071.36Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A430QPD94.338e-9967.68GTP-binding protein SAR1 OS=Schistosoma bovis OX=6... [more]
A0A183RFT34.840e-9967.17Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
G4V7094.840e-9967.17GTP-binding protein-like protein OS=Schistosoma ma... [more]
A0A183JBK67.831e-9967.17Uncharacterized protein OS=Schistosoma curassoni O... [more]
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BLAST of GTP-binding protein-like protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000056144.12.473e-8862.12pep primary_assembly:Astyanax_mexicanus-2.0:6:2204... [more]
sar1b5.765e-5365.57secretion associated, Ras related GTPase 1B [Sourc... [more]
ENSAMXT00000055305.14.603e-5164.91pep primary_assembly:Astyanax_mexicanus-2.0:6:2204... [more]
tysnd11.999e-4861.34trypsin domain containing 1 [Source:NCBI gene;Acc:... [more]
ENSAMXT00000046201.13.958e-2733.71ADP-ribosylation factor 4-like [Source:NCBI gene;A... [more]
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BLAST of GTP-binding protein-like protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
sar1b3.947e-8560.20secretion associated, Ras related GTPase 1B [Sourc... [more]
arf4a1.119e-2734.21ADP-ribosylation factor 4a [Source:ZFIN;Acc:ZDB-GE... [more]
arf4a1.299e-2734.21ADP-ribosylation factor 4a [Source:ZFIN;Acc:ZDB-GE... [more]
arl22.399e-2740.15ADP-ribosylation factor-like 2 [Source:ZFIN;Acc:ZD... [more]
ARF62.144e-2636.42ADP ribosylation factor 6 [Source:HGNC Symbol;Acc:... [more]
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BLAST of GTP-binding protein-like protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
SAR19.883e-7153.54ARF family GTPase; component of the COPII vesicle ... [more]
ARL11.338e-2632.22Soluble GTPase with a role in regulation of membra... [more]
ARF32.059e-2530.06Glucose-repressible ADP-ribosylation factor; GTPas... [more]
ARF12.548e-2529.71ADP-ribosylation factor; GTPase of the Ras superfa... [more]
ARF21.350e-2429.14ADP-ribosylation factor; GTPase of the Ras superfa... [more]
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BLAST of GTP-binding protein-like protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO457171.938e-8761.54Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO483924.577e-2832.39Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO483598.479e-2831.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO483601.457e-2731.58Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO426831.905e-2630.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of GTP-binding protein-like protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000030237.17.490e-8962.12secretion associated Ras related GTPase 1A [Source... [more]
sar1b1.720e-8862.63secretion associated Ras related GTPase 1B [Source... [more]
sar1ab1.191e-8661.50GTP-binding protein SAR1b [Source:NCBI gene;Acc:10... [more]
ENSORLT00000032693.13.708e-8354.91secretion associated Ras related GTPase 1A [Source... [more]
sar1b4.145e-7557.07secretion associated Ras related GTPase 1B [Source... [more]
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BLAST of GTP-binding protein-like protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000018556.13.052e-145100.00SMESG000018556.1[more]
SMESG000052759.17.242e-2834.97SMESG000052759.1[more]
SMESG000052764.17.555e-2834.97SMESG000052764.1[more]
SMESG000052741.12.362e-2743.22SMESG000052741.1[more]
SMESG000052739.12.570e-2743.22SMESG000052739.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003086 ID=SMED30003086|Name=GTP-binding protein-like protein|organism=Schmidtea mediterranea sexual|type=transcript|length=826bp
TTAGTATTCATCGTCACAATTCTGTTTTAATTTTGTTAATATAATTGAAA
ATGAGTGGACTTTGGGACTGGTTTTCTGGCACAGTTTCTGGTGTTCTCAG
TTATTTGGGTCTTTGGAAAAAATCTGGTAGGCTTGTTTTCCTTGGTTTAG
ATAACGCAGGAAAAACCACTATGCTTCATATGTTGAAGGACGATCGAATG
GCTACTCATAATCCGACACTTCATCCTACAACAGATGAATTAATTATAGG
TGGGATGAAATTCACTACTTATGACCTTGGTGGCCACGAACAGGCTCGAA
AAATTTGGAAAAATTATATGTCTTCAATAGATGGAATTGTGTTTTTAGTG
GATGCTTTTGATCAACAGAGATTACCAGAATCTCAGAAAGAATTGCAATT
TCTTATTCAAGATGATGAGATATCGAATGTTCCTTTATTAGTACTCGGAA
ATAAGATTGATAAAGGTGGTGCGTTAAATGAAGAAAATTTGCGATTTTAC
TTGGGAATTCAAAATTTAATAACTGGTAAAGGCAACGTTTCCAGACAAGA
ATTAGGTGGCCGCAGACCTTTGGAGTTATTCATGTGTTCAATTTTAAAAA
GGCAAGGATATGGAGAAGGCTTTAGATGGCTAGCAAACTACCTTTAATTT
TTTTGTTATTAAAACATTCAATAGATATCATTGATATATTAATTATCTTA
TAAAACTAGTAAATGAAAAGCTGTATATATTGTCTGATATTGATTTTGTT
TTGACTAGTAAAATGATGGATATGATAATTTTTTAATACAAATAACATTT
TCAAAATACTTGAGCATTATCTGTTA
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protein sequence of SMED30003086-orf-1

>SMED30003086-orf-1 ID=SMED30003086-orf-1|Name=SMED30003086-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=199bp
MSGLWDWFSGTVSGVLSYLGLWKKSGRLVFLGLDNAGKTTMLHMLKDDRM
ATHNPTLHPTTDELIIGGMKFTTYDLGGHEQARKIWKNYMSSIDGIVFLV
DAFDQQRLPESQKELQFLIQDDEISNVPLLVLGNKIDKGGALNEENLRFY
LGIQNLITGKGNVSRQELGGRRPLELFMCSILKRQGYGEGFRWLANYL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000035Category 3 cell
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR005225Small_GTP-bd_dom
IPR006689Small_GTPase_ARF/SAR
IPR027417P-loop_NTPase
IPR006687Small_GTPase_SAR1
Vocabulary: molecular function
TermDefinition
GO:0005525GTP binding
GO:0000166nucleotide binding
Vocabulary: cellular component
TermDefinition
GO:0005622intracellular
GO:0005783endoplasmic reticulum
GO:0005794Golgi apparatus
Vocabulary: biological process
TermDefinition
GO:0006886intracellular protein transport
GO:0006810transport
GO:0009792embryo development ending in birth or egg hatching
GO:0015031protein transport
GO:0016192vesicle-mediated transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006689Small GTPase superfamily, ARF/SAR typePRINTSPR00328SAR1GTPBPcoord: 55..79
score: 64.94
coord: 27..50
score: 76.96
coord: 127..148
score: 59.89
coord: 172..196
score: 64.47
coord: 82..107
score: 50.9
IPR006689Small GTPase superfamily, ARF/SAR typePFAMPF00025Arfcoord: 14..197
e-value: 1.2E-59
score: 200.7
NoneNo IPR availableSMARTSM00178sar_sub_1coord: 5..198
e-value: 6.4E-88
score: 308.1
NoneNo IPR availableSMARTSM00177arf_sub_2coord: 8..198
e-value: 6.1E-16
score: 69.0
NoneNo IPR availableGENE3DG3DSA:3.40.50.300coord: 26..198
e-value: 1.3E-55
score: 189.5
NoneNo IPR availablePANTHERPTHR45684:SF20GTP-BINDING PROTEIN SAR1Bcoord: 3..198
NoneNo IPR availablePANTHERPTHR45684FAMILY NOT NAMEDcoord: 3..198
NoneNo IPR availableCDDcd00879Sar1coord: 4..198
e-value: 1.73046E-112
score: 316.912
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 25..145
e-value: 2.0E-14
score: 51.7
IPR006687Small GTPase superfamily, SAR1-typePROSITEPS51422SAR1coord: 19..198
score: 26.173
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILYSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 22..198