NEK8-1
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30003066 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 5
Homology
BLAST of NEK8-1 vs. Ensembl Human
Match: NEK9 (NIMA related kinase 9 [Source:HGNC Symbol;Acc:HGNC:18591]) HSP 1 Score: 533.487 bits (1373), Expect = 1.965e-175 Identity = 285/702 (40.60%), Postives = 435/702 (61.97%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSL-SNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIING--EFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV-SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINS-NETEMINQFE-KITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR----CVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLG+GA+G+A LYR D+SLVVWKE+D+ + E + NEI IL++L H NI+AYYNHF+ T+ IE+EY N G+L LRQ + E V+WY FQ++ A+ IHK I+HRDIKTLNIFLT + L+K+GD+G++K LN E A T VGTP YMSPE+ QG+ Y+ KSDIWA GCV++E+++ K+ F+ +N L+L +KI+ G + + + +L+ ++ L +P +RP+A+++L P+ + + M ++V N R + ++V S SEV+VWGGGK K++ + SA QV AG +HF V+T EK+LYTW N+QG KL GQLGHG Y+ PK V+ L+G I++VSCG++ T+C++ G+++ GS+YYG +G++ E++ + +++P++ Q+SCG +H + +++ ++WGCGEYGRLGL E++ Y P VD+P + V CG D TF++ + S ++L CG N++NKLG+N Q +SG ++VP+ T F + L K+ T++ G +HTA +D +L FG N GQ+G+G+ K +N GK++ ++SCGD FTI +T +N IF WG +GRL + T C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 52 YIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQMVHSCLDQDPEQRPTADELLDRPLLRKRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIRQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVAGPEVLEPMQLNFFLSNPVE-------QVSCGDNHVVVLTRNKEV-YSWGCGEYGRLGLDSEEDYYTPQKVDVP-KALIIVAVQCGCDGTFLLTQ-SGKVLACGLNEFNKLGLN---QCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLGVGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGL 740
BLAST of NEK8-1 vs. Ensembl Human
Match: NEK8 (NIMA related kinase 8 [Source:HGNC Symbol;Acc:HGNC:13387]) HSP 1 Score: 324.324 bits (830), Expect = 6.407e-99 Identity = 214/665 (32.18%), Postives = 337/665 (50.68%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDID---KNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVH------RTNQANSNSRP-----------CSGDRK---ERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYKIPKLVQSL----KGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKV--FAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLG 2037
Y ++RV+G+GA+G L D LV+ K+I ++ K + QNE +L +L+HPN++ YY +F+ + I MEYA G+L+ ++ ++ L E +L +F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L+ A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ LL EP +RP ++ P+ C + +H R +A + P C G + +I S V+ WGGG L++ + +QVAAG + +T L W G L GA+ P+ + GV IK V+CG+ T CL+ G + GS G LG S + + T+ + + ++ Q++CG H A+ E LF WG G+ GRLGLG ++ P V +P + + + V CG D++ I+ + L CG+N++NKLG++ + G + + + F A L + ++ G +H+A + Y FG N +GQ+G T T + KV G K+ ++CGD FT+ +E++ WG+ GRLG
Sbjct: 4 YERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPL----CIRALLNLHTDVGSVRMRRAEKSVAPSNTGSRTTSVRCRGIPRGPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTE--VVQVAAGRTQKAGVTRSGRLILWEAPPLGAG-GGSLLPGAVEQPQPQFISRFLEGQSGVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHGS-----LTDISQPTIVEALLGYEMV--QVACGASHVLAL-STERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQ-EAQRVVCGIDSSMIL-TVPGQALACGSNRFNKLGLDHLSLGEEPVPHQQVEEALSF--TLLGSAPLDQEPLLSIDLGTAHSAAVTASGDCYTFGSNQHGQLG---TNTRRGSRAPCKVQGLEGIKMAMVACGDAFTVAIGAESEVYSWGKGARGRLG 646 HSP 2 Score: 58.151 bits (139), Expect = 5.367e-8 Identity = 33/99 (33.33%), Postives = 51/99 (51.52%), Query Frame = 1
Query: 1774 NVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFA--GKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRC 2064
V + V+CG+ TA L + FG S G +G G + D+++ + V A G ++ +++CG + +T E+F WGR DSGRLGL C
Sbjct: 390 GVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHG---SLTDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDSGRLGLGTRESHSC 485
BLAST of NEK8-1 vs. Ensembl Human
Match: NEK8 (NIMA related kinase 8 [Source:HGNC Symbol;Acc:HGNC:13387]) HSP 1 Score: 199.519 bits (506), Expect = 2.622e-55 Identity = 109/283 (38.52%), Postives = 168/283 (59.36%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDID---KNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDR 969
Y ++RV+G+GA+G L D LV+ K+I ++ K + QNE +L +L+HPN++ YY +F+ + I MEYA G+L+ ++ ++ L E +L +F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L+ A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ LL EP +RP ++ P+ C + +H + RP G R
Sbjct: 4 YERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPL----CIRALLNLHTDVGSVRMRRPVQGQR 282
BLAST of NEK8-1 vs. Ensembl Human
Match: NEK5 (NIMA related kinase 5 [Source:HGNC Symbol;Acc:HGNC:7748]) HSP 1 Score: 176.792 bits (447), Expect = 5.855e-49 Identity = 103/254 (40.55%), Postives = 150/254 (59.06%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHP 879
Y ++ +G+GA+GKA L + D+ V KEI+ +K I E + E+ +L + HPNIVA++N F +FI MEY + G L + RQ SE +L +F Q+ L +IH I+HRDIK NIFL+ + ++ K+GDFGI++VLN E A T +GTP Y+SPE+ Q PY++K+DIW+ GCVLYE+ + K FE +N L +KI F + + +L L+ +L P RPS +LK P
Sbjct: 4 YDVIKAIGQGAFGKAYLAKGKSDSKHCVIKEINFEKMPIQEKEASKKEVILLEKMKHPNIVAFFNSFQENGRLFIVMEYCDGGDLMKRINRQRGVLFSEDQILGWFVQISLGLKHIHDRKILHRDIKAQNIFLSKNGMVAKLGDFGIARVLNNSMELARTCIGTPYYLSPEICQNKPYNNKTDIWSLGCVLYELCTLKHPFEGNNLQQLVLKICQAHFAPISPGFSRELHSLISQLFQVSPRDRPSINSILKRP 257
BLAST of NEK8-1 vs. Ensembl Human
Match: NEK3 (NIMA related kinase 3 [Source:HGNC Symbol;Acc:HGNC:7746]) HSP 1 Score: 167.933 bits (424), Expect = 7.148e-46 Identity = 92/249 (36.95%), Postives = 156/249 (62.65%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKIN--EIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDL-SESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVL-NEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVL 870
Y+ +R++G+G++G+A+L ++ N + KEI + K+ N + E +L+ + HPNIVA+ F ++I MEY + G L ++Q K L E +L +F Q+ +++IHK ++HRDIK+ NIFLT + +K+GDFG +++L N FA T VGTP Y+ PE+ + LPY++KSDIW+ GC+LYE+ + K F+ ++ +L +K+ G +P+ + +L L++++ P+ RPSA +L
Sbjct: 4 YMVLRMIGEGSFGRALLVQHESSNQMFAMKEIRLPKSFSNTQNSRKEAVLLAKMKHPNIVAFKESFEAEGHLYIVMEYCDGGDLMQKIKQQKGKLFPEDMILNWFTQMCLGVNHIHKKRVLHRDIKSKNIFLTQNGKVKLGDFGSARLLSNPMAFACTYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCTLKHPFQANSWKNLILKVCQGCISPLPSHYSYELQFLVKQMFKRNPSHRPSATTLL 252
BLAST of NEK8-1 vs. Ensembl Celegans
Match: nekl-1 (NEK (NEver in mitosis Kinase) Like [Source:UniProtKB/TrEMBL;Acc:A0A061AD48]) HSP 1 Score: 287.73 bits (735), Expect = 1.202e-83 Identity = 233/766 (30.42%), Postives = 371/766 (48.43%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEI---DIDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFAN---TQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAI---GGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVY--CGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVA--------VLRNV--KVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHT-KTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRCVLHPRALFGTLQKCADIQSFGWQTIMIT------ERILETSTISANSTAI---MDKLSLKNVNSFETEYDKDGSGD------IQTWLRKELQE-DVIYMD 2316
Y ++R +GKGA+G AVLYR D+SLV+ KEI D+D ++ NE+ +LS ++HPNI+AYY+ F + IEMEYA+ G+L+ L + + L E + Q+ A+ Y+H+ ++HRD+KT N+FLT + +KIGDFGISK++ + A T VGTP Y+SPE+ G+ Y+ KSD+WA GC+LYE+ KK FE N +L I+ + + ++++ +++ LL +P KRPSA LK M+ ++ S S RS VS + ++ I I + S QVA +H +VLT + +L+ + GD GQLG G + +LV+SLKG I+ V G ++ G + G+ LG ++ ++ E++ + I I CGH H A+ G C WG +YG+LGL ++ + PT + +P +K K+VY G D T ++ L+ G+N++NKL NL V F TK K +L+ +V S G+ H+ V+ ++ IFG N ++GLG++ K K K + CGD FT+VSTT E++ WGR D + I + V + GT + D ++++ ++ + S ++ + + +D L K + S E + GS TWLR+E ++ ++I +D
Sbjct: 133 YERIRTVGKGAFGSAVLYRRREDSSLVIIKEINMYDLDSSQRRLALNEVSLLSRIEHPNIIAYYDSFEEEGVLMIEMEYADGGTLAQMLSRTQNLLDEEQIGDMMIQMCSAVAYLHENSVLHRDLKTANVFLTRDSFVKIGDFGISKIMGTETLAQGAKTVVGTPYYISPEMCSGVSYNEKSDMWALGCILYEMCCLKKAFEGDNLPALVNSIMTCAYTPVKGPYSAEMKMVIRELLQLDPQKRPSAPQALK----------MLRPSENRHRHTSGSM--------RSSVSFTT-LYDLHVPTITLSSIPDLPSRISIKQVALSKTHTMVLTNDNELFGF----GDNS-CGQLGFGKFSKMENGRLVESLKGRDIQSVGVGNYFSVICCDRGTLLTFGTAKNLGLGKDAKDSAKPMLLEQLLREN--------IKDIYCGHDHVVAVLESGEC----LGWGSNQYGQLGLPTLEHFFAPTKIPMPSNK---KIVYGKAGKDATMLLSD-DGSLIAMGSNKHNKL---------------NLTQRVGFFTKEKKTPNDYVNKPTILQGFPERVVDFSVGSYHSGVILESGQVMIFGRNDNTELGLGNSQKMAVRCVKPVKSLLHHACTHVLCGDGFTLVSTTEQELYFWGRKDLTDEDVCIDDLEETVTEKKMKRGTTTEIFDKNVVTLPSLILRLQDERGDKGIRLSGLAVSGRKVHVAVDMLEGKRLPSALAERCRSGSAPAINDETTHTWLREEFEKAEIIPID 843
BLAST of NEK8-1 vs. Ensembl Celegans
Match: nekl-1 (NEK (NEver in mitosis Kinase) Like [Source:UniProtKB/TrEMBL;Acc:A0A061AD48]) HSP 1 Score: 287.73 bits (735), Expect = 2.390e-83 Identity = 233/766 (30.42%), Postives = 371/766 (48.43%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEI---DIDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFAN---TQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAI---GGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVY--CGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIV--------AVLRNV--KVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHT-KTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRCVLHPRALFGTLQKCADIQSFGWQTIMIT------ERILETSTISANSTAI---MDKLSLKNVNSFETEYDKDGSGD------IQTWLRKELQE-DVIYMD 2316
Y ++R +GKGA+G AVLYR D+SLV+ KEI D+D ++ NE+ +LS ++HPNI+AYY+ F + IEMEYA+ G+L+ L + + L E + Q+ A+ Y+H+ ++HRD+KT N+FLT + +KIGDFGISK++ + A T VGTP Y+SPE+ G+ Y+ KSD+WA GC+LYE+ KK FE N +L I+ + + ++++ +++ LL +P KRPSA LK M+ ++ S S RS VS + ++ I I + S QVA +H +VLT + +L+ + GD GQLG G + +LV+SLKG I+ V G ++ G + G+ LG ++ ++ E++ + I I CGH H A+ G C WG +YG+LGL ++ + PT + +P +K K+VY G D T ++ L+ G+N++NKL NL V F TK K +L+ +V S G+ H+ V+ ++ IFG N ++GLG++ K K K + CGD FT+VSTT E++ WGR D + I + V + GT + D ++++ ++ + S ++ + + +D L K + S E + GS TWLR+E ++ ++I +D
Sbjct: 171 YERIRTVGKGAFGSAVLYRRREDSSLVIIKEINMYDLDSSQRRLALNEVSLLSRIEHPNIIAYYDSFEEEGVLMIEMEYADGGTLAQMLSRTQNLLDEEQIGDMMIQMCSAVAYLHENSVLHRDLKTANVFLTRDSFVKIGDFGISKIMGTETLAQGAKTVVGTPYYISPEMCSGVSYNEKSDMWALGCILYEMCCLKKAFEGDNLPALVNSIMTCAYTPVKGPYSAEMKMVIRELLQLDPQKRPSAPQALK----------MLRPSENRHRHTSGSM--------RSSVSFTT-LYDLHVPTITLSSIPDLPSRISIKQVALSKTHTMVLTNDNELFGF----GDNS-CGQLGFGKFSKMENGRLVESLKGRDIQSVGVGNYFSVICCDRGTLLTFGTAKNLGLGKDAKDSAKPMLLEQLLREN--------IKDIYCGHDHVVAVLESGEC----LGWGSNQYGQLGLPTLEHFFAPTKIPMPSNK---KIVYGKAGKDATMLLSD-DGSLIAMGSNKHNKL---------------NLTQRVGFFTKEKKTPNDYVNKPTILQGFPERVVDFSVGSYHSGVILESGQVMIFGRNDNTELGLGNSQKMAVRCVKPVKSLLHHACTHVLCGDGFTLVSTTEQELYFWGRKDLTDEDVCIDDLEETVTEKKMKRGTTTEIFDKNVVTLPSLILRLQDERGDKGIRLSGLAVSGRKVHVAVDMLEGKRLPSALAERCRSGSAPAINDETTHTWLREEFEKAEIIPID 881
BLAST of NEK8-1 vs. Ensembl Celegans
Match: nekl-2 (Serine/threonine-protein kinase nekl-2 [Source:UniProtKB/Swiss-Prot;Acc:O01775]) HSP 1 Score: 155.606 bits (392), Expect = 2.704e-41 Identity = 93/263 (35.36%), Postives = 154/263 (58.56%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNS--LVVWKEID---IDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQ--------LKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTT-STLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPV 882
Y +VRV+G+GA+G L R D S V+ K I+ + + + N IQ+E+++L + HP I+ Y + F+ + + I M+YA G+L + ++E E VL YF Q++ AL+++H+ +I+HRD+K NI + T+LK+ DFGISK L A+T +GTP Y+SPE+ + PY+ KSD+W+ GCVLYE++ ++ F+ N ++ MKI + + + ++D+ L++ LL KRP +L P+
Sbjct: 4 YEKVRVVGRGAFGVCWLCRGKNDASHQKVIIKLINTHGMTEKEENSIQSEVNLLKKVQHPLIIGYIDSFIMDNQLGIVMQYAEGGTLERLINDQRAIKDSNMREYFPEKTVLDYFTQILIALNHMHQKNIVHRDLKPQNILMNRRKTVLKLSDFGISKELGTKSAASTVIGTPNYLSPEICESRPYNQKSDMWSLGCVLYELLQLERAFDGENLPAIVMKITRSKQNPLGDHVSNDVKMLVENLLKTHTDKRPDVSQLLSDPL 266
BLAST of NEK8-1 vs. Ensembl Celegans
Match: nekl-3 (Serine/threonine-protein kinase nekl-3 [Source:UniProtKB/Swiss-Prot;Acc:G5EFM9]) HSP 1 Score: 134.035 bits (336), Expect = 1.995e-34 Identity = 88/262 (33.59%), Postives = 150/262 (57.25%), Query Frame = 1
Query: 121 LNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDI----DKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKED---LSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLF--EYSNHLSLAMKIINGEFQTMP-NCNNSXXXXXXXXXXXNEPTKRPSAEDVLK 873
L++ ++ + +GKG + + + + V K+I + D+ + EID+L L+H N++ YY F+ + + I +E A AG +S ++ K+ + E + YF QL AL ++H I+HRDIK N+F+T + ++K+GD G+ + + + A++ VGTP YMSPE IQ Y+ KSD+W++GC+LYE+ + + F + N SL KI N E+ +P + ++ L DL+ R +L E +KRP +VL+
Sbjct: 18 LSLELFIIEKKIGKGQFSEVFRAQCTWVDLHVALKKIQVFEMVDQKARQDCLKEIDLLKQLNHVNVIRYYASFIDNNQLNIVLELAEAGDMSRMIKHFKKGGRLIPEKTIWKYFVQLARALAHMHSKRIMHRDIKPANVFITGNGIVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIQESGYNFKSDLWSTGCLLYEMAALQSPFYGDKMNLYSLCKKIENCEYPPLPADIYSTQLRDLVSRCILPEASKRPETSEVLQ 279
BLAST of NEK8-1 vs. Ensembl Celegans
Match: nekl-3 (Serine/threonine-protein kinase nekl-3 [Source:UniProtKB/Swiss-Prot;Acc:G5EFM9]) HSP 1 Score: 134.035 bits (336), Expect = 1.995e-34 Identity = 88/262 (33.59%), Postives = 150/262 (57.25%), Query Frame = 1
Query: 121 LNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDI----DKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKED---LSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLF--EYSNHLSLAMKIINGEFQTMP-NCNNSXXXXXXXXXXXNEPTKRPSAEDVLK 873
L++ ++ + +GKG + + + + V K+I + D+ + EID+L L+H N++ YY F+ + + I +E A AG +S ++ K+ + E + YF QL AL ++H I+HRDIK N+F+T + ++K+GD G+ + + + A++ VGTP YMSPE IQ Y+ KSD+W++GC+LYE+ + + F + N SL KI N E+ +P + ++ L DL+ R +L E +KRP +VL+
Sbjct: 18 LSLELFIIEKKIGKGQFSEVFRAQCTWVDLHVALKKIQVFEMVDQKARQDCLKEIDLLKQLNHVNVIRYYASFIDNNQLNIVLELAEAGDMSRMIKHFKKGGRLIPEKTIWKYFVQLARALAHMHSKRIMHRDIKPANVFITGNGIVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIQESGYNFKSDLWSTGCLLYEMAALQSPFYGDKMNLYSLCKKIENCEYPPLPADIYSTQLRDLVSRCILPEASKRPETSEVLQ 279
BLAST of NEK8-1 vs. Ensembl Fly
Match: niki (gene:FBgn0045980 transcript:FBtr0084737) HSP 1 Score: 263.077 bits (671), Expect = 2.878e-75 Identity = 206/672 (30.65%), Postives = 321/672 (47.77%), Query Frame = 1
Query: 127 VVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDK---NKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL--RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKH-------------------------------PVWKGNCQSMIDQVH-RTNQANSNSRPCSGDRKERSIVSLNSEVF-VWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAI-VYK-IPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYK-PTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKT--HKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWG 2013
+ +Y +VRV+G+G++G A+LYR D +V+K+I++ + + NE+D+ S L HPNIV+Y F+ +T+ IEMEYA+ G+L+ + RQ K E ++ F Q+ A++Y+H +I+HRD+KT N+FL ++KIGDFGISK++N A T +GTP Y SPE+ +G Y +KSDIWA GC+L E+ KK F SN L KI+ G + +P+ S L L+ LL E +RP+A +VL + PV ++ ++ T Q + + I+ S ++ + G ++ Q K + VA SHFVV+ + Y W + GQLG A+ +K P ++S++ + G+ TI ++ G + CGSN + LG + E N + D V + Q++ G H A+ E ++ WG G LGLG+ K P + + K K +YCG DT+ ++ + L CG+N YNKLG A+ T FK V + KV+ ++H+ L +Y G N+ GQ G+ H + H + S K + I K +C D TIV++ +N I WG
Sbjct: 102 LANYEKVRVVGQGSFGIAILYRRKSDGHQIVFKQINLSELSPPGRDLAMNEVDVFSKLHHPNIVSYLGSFIKDNTLLIEMEYADGGTLAQIIAERQGKLHFPERYIIAVFEQISSAINYMHSENILHRDLKTANVFLNRRGIVKIGDFGISKIMNTKIHAQTVLGTPYYFSPEMCEGKEYDNKSDIWALGCILGEMCCLKKTFAASNLSELVTKIMAGNYTPVPSGYTSGLRSLMSNLLQVEAPRRPTASEVLVYWIPLIFRSLGKNKGYSYEDDVGGPGSDQLTAPVPAAAYSNVSMELELPTAQTETKQLMIADTAAPHEILEKRSVLYQLKAFGTCFSMAPIQLPPKAVIVDVAMSDSHFVVVNEDGSAYAWG-----EGTHGQLGLTALEAWKHYPSRMESVRNYHVVSACAGDGFTILVTQAGSLLSCGSNAHLALG----QDEQRNYHSPKLIARLAD---VRVEQVAAGLQHVLALSR-EGAVYVWGTSTCGALGLGNYQQQQKFPQKILLSHVKTKPSKIYCGPDTSAVLF-ANGELHVCGSNDYNKLGFQRSAK----------------ITAFKKVQLPH--KVTQACFSSTHSVFLVEGGYVYTMGRNAEGQRGIRHCNSVDHPTLVDSVK---SRYIVKANCSDQCTIVASEDNIITVWG 738 HSP 2 Score: 52.373 bits (124), Expect = 1.828e-6 Identity = 52/191 (27.23%), Postives = 84/191 (43.98%), Query Frame = 1
Query: 1477 FTWGCGEYGRLGL-GHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLG-HTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLE 2043
+ WG G +G+LGL E + P+ ++ + + C D I+ + LL CG+N + LG + Q+N Y P K++A L +V+V V+ G H L +Y++G ++ G +GLG + + K K K KI CG + V N E+ G D +LG +
Sbjct: 474 YAWGEGTHGQLGLTALEAWKHYPSRMESVRNYHVVSA--CAGDGFTILVTQAGSLLSCGSNAHLALGQD---------EQRN--YHSP-----KLIARLADVRVEQVAAGLQHVLALSREGAVYVWGTSTCGALGLGNYQQQQKFPQKILLSHVKTKPSKIYCGPDTSAVLFANGELHVCGSNDYNKLGFQ 646
BLAST of NEK8-1 vs. Ensembl Fly
Match: Nek2 (gene:FBgn0029970 transcript:FBtr0071142) HSP 1 Score: 159.844 bits (403), Expect = 2.885e-40 Identity = 96/272 (35.29%), Postives = 151/272 (55.51%), Query Frame = 1
Query: 106 DFSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEI---DIDKNKINEIQNEIDILSILDHPNIVAYYNHFVYID--TVFIEMEYANAGSLSNYL---RQLKEDLSESCVLWYFFQLIYALDYIHKL----HIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDE-FANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPV 882
DFS + Y + V+G G++G R+ L WK + ++D+ K + + +EI +L L HPNIV YY+H V + +V+I ME G L+ + R ++ E + FQL AL H I+HRDIK NIFL + K+GDFG++++L D+ FA + VGTP YMSPE+++G Y KSD+WA GC++YE+ + + F L+ KI GEF +P ++DL +++ +L + +RP E +++HP+
Sbjct: 10 DFSQK-TLQDYEVLAVMGNGSFGTCYKVRDKSTGELFAWKGMNYDELDEAKCDALVSEISVLRQLQHPNIVQYYHHLVNREAKSVYIVMECCAGGDLAQIVQRARSQRQRFEEPYIWRVLFQLCRALQVCHNKIPNGTILHRDIKPANIFLDAAGNAKLGDFGLARMLRRDQSFAASFVGTPHYMSPELVKGRKYDRKSDVWAVGCLVYEMCALRPPFRGRAFDQLSEKIAQGEFSRIPAIYSTDLQEIIAFMLAVDHEQRPGIEVIIRHPL 280
BLAST of NEK8-1 vs. Ensembl Fly
Match: Sik2 (gene:FBgn0025625 transcript:FBtr0070340) HSP 1 Score: 131.339 bits (329), Expect = 9.722e-31 Identity = 83/261 (31.80%), Postives = 145/261 (55.56%), Query Frame = 1
Query: 148 RVLGKGAYGKAVLYRNAVDNSLVVWKEID---IDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKS-DIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQV 918
R +GKG + L R+ + + V K ID +D+ + ++ E++I+ L HP+I+ Y + ++I EYA+ G + +Y+ + +SES + F+Q+I A++Y HK I+HRD+K N+ L + +KI DFG S E T G+P Y +PEV +G Y+ DIW+ G VLY ++ F+ S SL ++++G F+ +P +S+ L++R+L+ EPT+R + + + +H W C +++ V
Sbjct: 145 RTIGKGNFAVVKLARHRITKNEVAIKIIDKSQLDQTNLQKVYREVEIMKRLKHPHIIKLYQVMETKNMIYIVSEYASQGEIFDYIAKYGR-MSESAARFKFWQIISAVEYCHKKGIVHRDLKAENLLLDLNMNIKIADFGFSNHFKPGELLATWCGSPPYAAPEVFEGKQYTGPEIDIWSLGVVLYVLVCGALPFDGSTLQSLRDRVLSGRFR-IPFFMSSECEHLIRRMLVLEPTRRYTIDQIKRH-RWM--CPELLEHV 400
BLAST of NEK8-1 vs. Ensembl Fly
Match: AMPKalpha (gene:FBgn0023169 transcript:FBtr0070216) HSP 1 Score: 121.324 bits (303), Expect = 2.621e-28 Identity = 80/252 (31.75%), Postives = 134/252 (53.17%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNK----INEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKS-DIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKH 876
Y+ LG G +GK + + + V K ++ K K + +I+ EI L + HP+I+ Y +F+ MEY + G L +Y+ + L E +F Q+I +DY H+ I+HRD+K N+ L + +KI DFG+S ++ + EF T G+P Y +PEVI G Y+ DIW+ G +LY ++ F+ + +L KI +G F +P N +++L+ ++L +P KR + E++ KH
Sbjct: 28 YLLGATLGTGTFGKVKIGEHQITRVKVAVKILNRQKIKSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMIMEYVSGGELFDYIVK-HGKLQEHQARRFFQQIISGVDYCHRHMIVHRDLKPENLLLDHNMHVKIADFGLSNMMLDGEFLRTSCGSPNYAAPEVISGKLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFRKIKSGIF-PIPEYLNKQVVNLVCQMLQVDPLKRANIEEIKKH 277
BLAST of NEK8-1 vs. Ensembl Fly
Match: AMPKalpha (gene:FBgn0023169 transcript:FBtr0070217) HSP 1 Score: 121.324 bits (303), Expect = 2.621e-28 Identity = 80/252 (31.75%), Postives = 134/252 (53.17%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNK----INEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKS-DIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKH 876
Y+ LG G +GK + + + V K ++ K K + +I+ EI L + HP+I+ Y +F+ MEY + G L +Y+ + L E +F Q+I +DY H+ I+HRD+K N+ L + +KI DFG+S ++ + EF T G+P Y +PEVI G Y+ DIW+ G +LY ++ F+ + +L KI +G F +P N +++L+ ++L +P KR + E++ KH
Sbjct: 28 YLLGATLGTGTFGKVKIGEHQITRVKVAVKILNRQKIKSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMIMEYVSGGELFDYIVK-HGKLQEHQARRFFQQIISGVDYCHRHMIVHRDLKPENLLLDHNMHVKIADFGLSNMMLDGEFLRTSCGSPNYAAPEVISGKLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFRKIKSGIF-PIPEYLNKQVVNLVCQMLQVDPLKRANIEEIKKH 277
BLAST of NEK8-1 vs. Ensembl Zebrafish
Match: nek8 (NIMA-related kinase 8 [Source:NCBI gene;Acc:171094]) HSP 1 Score: 327.405 bits (838), Expect = 1.141e-99 Identity = 221/678 (32.60%), Postives = 354/678 (52.21%), Query Frame = 1
Query: 109 FSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINE---IQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKH-----PVWK-----GNCQ-SMIDQVHRTNQANSNSRP---CSGDRKERSIVSLN----------SEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTW---SNIQGDKKLSGQLGHGAIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGI-NSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIV--AVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLG 2037
F + N+ Y +++V+G+GA+G L R D++LV+ KEI +++ +E QNE +L +L HPNI+ YY +F+ + I MEYA G+L++Y+ ++ L E +L +F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ +L +P+KRP +++ H P+ GN + I++ QA + RP + R + SL S V+ WG G L++ + +QV+ G + + +T L TW S G+ L G + + I + ++ GV IK VSCG+ T CL+ G + GS G LG N N+ E + + + Q+SCG H A+ E +F+WG G+ GRLGL +D+ P V +P + + V CG D + I+ ++L CGNN++NKLG++ +SG+ + + V F++V A L K+ + G +H+ + + + FG N +GQ+G H ++ + + S + + I +CGD FT+ E++ WG+ GRLG
Sbjct: 41 FGASENMEKYEKIKVVGRGAFGIVHLCRRRTDSALVILKEIPVEQMTRDERLAAQNECQVLKLLSHPNIIEYYENFLEDKALMIAMEYAPGGTLADYIQKRCNSLLDEDTILHFFVQILLALYHVHNKLILHRDLKTQNILLDKHQMIVKIGDFGISKILVSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLILNMLNLDPSKRPQLNEIMAHAICIRPLLNLYTDIGNVKMRRIEKPLSNVQAGPHGRPGGWITSTRTRGGLSSLTSSKMMHPLPLSSVYTWGSGISTPLRLPMLNTE--VIQVSLGRTQKMGVTKSGRLITWEAPSVGSGEPTLPGAVEQMQPQF-ISRFLEGQSGVTIKSVSCGDLFTTCLTDRGIIMTFGSGSNGCLGHGNFNDVTQPKIVEALLGYE--------LVQVSCGASHVLAVTN-EREVFSWGRGDNGRLGLATQDSHNCPQQVSLP-ADFEAQRVLCGVDCSMIM-STQHQILACGNNRFNKLGLD----KVSGTEEPSSFCQVEEVHLFQLVQSAPLNTEKIVYIDIGTAHSVAVTEKGQCFTFGSNQHGQLGCSHRRSSRVPYQVSGL---QGITMAACGDAFTLAIGAEGEVYTWGKGARGRLG 697 HSP 2 Score: 57.3806 bits (137), Expect = 6.633e-8 Identity = 34/98 (34.69%), Postives = 48/98 (48.98%), Query Frame = 1
Query: 1777 VKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFA--GKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRC 2064
V + +VSCG+ T L + FG S G +G G+ DV + V A G ++ ++SCG + T E+F WGR D+GRLGL C
Sbjct: 444 VTIKSVSCGDLFTTCLTDRGIIMTFGSGSNGCLGHGN---FNDVTQPKIVEALLGYELVQVSCGASHVLAVTNEREVFSWGRGDNGRLGLATQDSHNC 538
BLAST of NEK8-1 vs. Ensembl Zebrafish
Match: nek8 (NIMA-related kinase 8 [Source:NCBI gene;Acc:171094]) HSP 1 Score: 323.939 bits (829), Expect = 6.634e-99 Identity = 219/669 (32.74%), Postives = 350/669 (52.32%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINE---IQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKH-----PVWK-----GNCQ-SMIDQVHRTNQANSNSRP---CSGDRKERSIVSLN----------SEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTW---SNIQGDKKLSGQLGHGAIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGI-NSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIV--AVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLG 2037
Y +++V+G+GA+G L R D++LV+ KEI +++ +E QNE +L +L HPNI+ YY +F+ + I MEYA G+L++Y+ ++ L E +L +F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ +L +P+KRP +++ H P+ GN + I++ QA + RP + R + SL S V+ WG G L++ + +QV+ G + + +T L TW S G+ L G + + I + ++ GV IK VSCG+ T CL+ G + GS G LG N N+ E + + + Q+SCG H A+ E +F+WG G+ GRLGL +D+ P V +P + + V CG D + I+ ++L CGNN++NKLG++ +SG+ + + V F++V A L K+ + G +H+ + + + FG N +GQ+G H ++ + + S + + I +CGD FT+ E++ WG+ GRLG
Sbjct: 4 YEKIKVVGRGAFGIVHLCRRRTDSALVILKEIPVEQMTRDERLAAQNECQVLKLLSHPNIIEYYENFLEDKALMIAMEYAPGGTLADYIQKRCNSLLDEDTILHFFVQILLALYHVHNKLILHRDLKTQNILLDKHQMIVKIGDFGISKILVSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLILNMLNLDPSKRPQLNEIMAHAICIRPLLNLYTDIGNVKMRRIEKPLSNVQAGPHGRPGGWITSTRTRGGLSSLTSSKMMHPLPLSSVYTWGSGISTPLRLSMLNTE--VIQVSLGRTQKMGVTKSGRLITWEAPSVGSGEPTLPGAVEQMQPQF-ISRFLEGQSGVTIKSVSCGDLFTTCLTDRGIIMTFGSGSNGCLGHGNFNDVTQPKIVEALLGYELV--------QVSCGASHVLAVTN-EREVFSWGRGDNGRLGLATQDSHNCPQQVSLPA-DFEAQRVLCGVDCSMIM-STQHQILACGNNRFNKLGLD----KVSGTEEPSSFCQVEEVHLFQLVQSAPLNTEKIVYIDIGTAHSVAVTEKGQCFTFGSNQHGQLGCSHRRSSRVPYQVSGL---QGITMAACGDAFTLAIGAEGEVYTWGKGARGRLG 651 HSP 2 Score: 57.3806 bits (137), Expect = 6.797e-8 Identity = 34/98 (34.69%), Postives = 48/98 (48.98%), Query Frame = 1
Query: 1777 VKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFA--GKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRC 2064
V + +VSCG+ T L + FG S G +G G+ DV + V A G ++ ++SCG + T E+F WGR D+GRLGL C
Sbjct: 398 VTIKSVSCGDLFTTCLTDRGIIMTFGSGSNGCLGHGN---FNDVTQPKIVEALLGYELVQVSCGASHVLAVTNEREVFSWGRGDNGRLGLATQDSHNC 492
BLAST of NEK8-1 vs. Ensembl Zebrafish
Match: nek1 (NIMA-related kinase 1 [Source:ZFIN;Acc:ZDB-GENE-040730-1]) HSP 1 Score: 173.326 bits (438), Expect = 4.106e-48 Identity = 99/253 (39.13%), Postives = 149/253 (58.89%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDK---NKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDL-SESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHP 879
Y +++ +G+G++GKA+L ++ D V KEI I + + E + E+ +L+ + HPNIV Y F ++I M+Y G L + + L E +L +F Q+ AL ++H I+HRDIK+ NIFLT +++GDFGI++VLN E A T +GTP Y+SPE+ + PY++KSDIWA GCVLYE+ + K FE N +L +KII G + + + DL LL +L P +RPS +L P
Sbjct: 4 YERLKKIGEGSFGKAILVKSRTDGRQYVIKEIGISRMSNKERQESRKEVAVLANMSHPNIVQYKESFEESGCLYIVMDYCEGGDLFKKINNQRGSLFPEEQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSIHYSPDLRSLLAQLFKRNPRERPSVSTILDKP 256
BLAST of NEK8-1 vs. Ensembl Zebrafish
Match: nek12 (NIMA-related kinase 12 [Source:ZFIN;Acc:ZDB-GENE-050410-11]) HSP 1 Score: 176.407 bits (446), Expect = 4.251e-47 Identity = 103/283 (36.40%), Postives = 169/283 (59.72%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQN----EIDILSILDHPNIVAYYNHFVYI--DTVFIEMEYANAGSLSNYLRQLK--EDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMI-DQVHRTNQANSNSRP 954
Y +V LG+G G L ++ L K I +D ++ + ++ E +L L HP+I+ + + F ++++I M+Y + G+L +++++ K E +ES ++ +F Q++ A+ YIH I+HRDIK N+ LT ++K+GDFGISK++N + A+T VGTP Y+SPE+ Q +PYS+KSDIWA GC+LYE+ + K F+ N LSL KII GE+ +P + D+ L++++L P RPSA +L + + + I +Q + N+ SRP
Sbjct: 4 YDKVLTLGQGGAGVVSLMKHVDTKKLCAVKRIHVDSSRKTKTKDVVLQEAIVLRSLKHPHIITWIDTFFDATNESIYIVMDYCDGGTLDDHIKEQKCGEHFTESNIMDWFVQVVMAVSYIHSEKILHRDIKPSNVLLTKRGVIKLGDFGISKIMNHTLDMASTCVGTPSYLSPELCQDVPYSTKSDIWAVGCLLYELCALKPAFQAKNLLSLFYKIIKGEYIKVPEMYSEDIHTLIEKMLSLIPENRPSAASILSMTYVQEHLGNFITNQEKELTKVNAESRP 286
BLAST of NEK8-1 vs. Ensembl Zebrafish
Match: nek12 (NIMA-related kinase 12 [Source:ZFIN;Acc:ZDB-GENE-050410-11]) HSP 1 Score: 176.407 bits (446), Expect = 4.251e-47 Identity = 103/283 (36.40%), Postives = 169/283 (59.72%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQN----EIDILSILDHPNIVAYYNHFVYI--DTVFIEMEYANAGSLSNYLRQLK--EDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMI-DQVHRTNQANSNSRP 954
Y +V LG+G G L ++ L K I +D ++ + ++ E +L L HP+I+ + + F ++++I M+Y + G+L +++++ K E +ES ++ +F Q++ A+ YIH I+HRDIK N+ LT ++K+GDFGISK++N + A+T VGTP Y+SPE+ Q +PYS+KSDIWA GC+LYE+ + K F+ N LSL KII GE+ +P + D+ L++++L P RPSA +L + + + I +Q + N+ SRP
Sbjct: 4 YDKVLTLGQGGAGVVSLMKHVDTKKLCAVKRIHVDSSRKTKTKDVVLQEAIVLRSLKHPHIITWIDTFFDATNESIYIVMDYCDGGTLDDHIKEQKCGEHFTESNIMDWFVQVVMAVSYIHSEKILHRDIKPSNVLLTKRGVIKLGDFGISKIMNHTLDMASTCVGTPSYLSPELCQDVPYSTKSDIWAVGCLLYELCALKPAFQAKNLLSLFYKIIKGEYIKVPEMYSEDIHTLIEKMLSLIPENRPSAASILSMTYVQEHLGNFITNQEKELTKVNAESRP 286
BLAST of NEK8-1 vs. Ensembl Xenopus
Match: nek9 (NIMA-related kinase 9 [Source:NCBI gene;Acc:100216232]) HSP 1 Score: 534.643 bits (1376), Expect = 1.291e-176 Identity = 294/706 (41.64%), Postives = 431/706 (61.05%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSL-SNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXX----XNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV---SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR------CVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLG GAYG+A LYR D+SLVVWKE+ + + E + NEI ILS+L H NI+AYYNHF+ +T+ IE+EY N G+L +RQ + E VLWY FQ++ A+ IH+ I+HRDIKTLNIFLT + L+K+GD+G++K L+ E A T VGT YMSPE+ QG+ YS KSDIWA GCVLYE+++ + F+ +N L+L +KI+ G + +NS L ++ +P KRP+A+++LK P+ + M ++V N+ SN +P +G E I S +SEV+VWGGGK K++ F+ A QV AG +HF V+T EK+LYTW N+QG KL GQLGHG Y+ PK V+ L+G +++VSCG + T+C+S G+++ GS+YYG LG+N + + E + V D PV Q+SCG H A+ + +++WGCGEYGRLGL ED++Y P V++ C + V CG D +F++ + ++L CG N++NKLG+N + G+ +VP+ T + L K+ ++S G +HTA +D +L FG N GQ+G+G + H +N GK++ ++SCGD FTI +T +N IF WG +GRL + T ++R C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 34 YIPIRVLGHGAYGEATLYRRTEDDSLVVWKEVGLARLSEKERRDALNEIVILSLLQHDNIIAYYNHFLDSNTLLIELEYCNGGNLFDKIVRQKAQLFQEEMVLWYLFQIVSAVSCIHRAGILHRDIKTLNIFLTKANLIKLGDYGLAKQLSSEYSMAETCVGTLYYMSPELCQGVKYSFKSDIWAVGCVLYELLTLTRTFDATNPLNLCVKIVQGNWAM--GLDNSVYSQDLIEVVHACLEQDPEKRPTADEILKMPILSWRRRDMEEKVSMLNR--SNKKPRTGTVTEAPIAVVTSRSSEVYVWGGGKTTPQKLDVFKGGCRARQVCAGDAHFAVVTVEKELYTWVNMQGGSKLHGQLGHGDRASYRQPKHVEKLQGKSVQQVSCGSDFTVCISDEGQLYSFGSDYYGCLGVNQSAGAEV--LEPLLV-DFFLNEPV--EQVSCGDSHIIALTRSK-CVYSWGCGEYGRLGLDSEDDVYSPQKVEVQRDLCIVN-VCCGSDGSFLL-TLTGKVLACGLNEHNKLGLNQYTAGII---NHEAFQEVPYTTSLTLAKQLSFYKIRSISPGRTHTAAIDERGRLLTFGSNKCGQLGVGDYRKHLGINLLGGPLGGKQVIRVSCGDEFTIAATADNHIFAWGNGGNGRLAM--TPNERPQGSDICTSWPRPIFGSLHHVTDLSCRGWHTILIVEKVLNSKTIRSNSSGL 722
BLAST of NEK8-1 vs. Ensembl Xenopus
Match: nek9 (NIMA-related kinase 9 [Source:NCBI gene;Acc:100216232]) HSP 1 Score: 533.872 bits (1374), Expect = 3.471e-176 Identity = 294/706 (41.64%), Postives = 431/706 (61.05%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSL-SNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXX----XNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV---SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR------CVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLG GAYG+A LYR D+SLVVWKE+ + + E + NEI ILS+L H NI+AYYNHF+ +T+ IE+EY N G+L +RQ + E VLWY FQ++ A+ IH+ I+HRDIKTLNIFLT + L+K+GD+G++K L+ E A T VGT YMSPE+ QG+ YS KSDIWA GCVLYE+++ + F+ +N L+L +KI+ G + +NS L ++ +P KRP+A+++LK P+ + M ++V N+ SN +P +G E I S +SEV+VWGGGK K++ F+ A QV AG +HF V+T EK+LYTW N+QG KL GQLGHG Y+ PK V+ L+G +++VSCG + T+C+S G+++ GS+YYG LG+N + + E + V D PV Q+SCG H A+ + +++WGCGEYGRLGL ED++Y P V++ C + V CG D +F++ + ++L CG N++NKLG+N + G+ +VP+ T + L K+ ++S G +HTA +D +L FG N GQ+G+G + H +N GK++ ++SCGD FTI +T +N IF WG +GRL + T ++R C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 34 YIPIRVLGHGAYGEATLYRRTEDDSLVVWKEVGLARLSEKERRDALNEIVILSLLQHDNIIAYYNHFLDSNTLLIELEYCNGGNLFDKIVRQKAQLFQEEMVLWYLFQIVSAVSCIHRAGILHRDIKTLNIFLTKANLIKLGDYGLAKQLSSEYSMAETCVGTLYYMSPELCQGVKYSFKSDIWAVGCVLYELLTLTRTFDATNPLNLCVKIVQGNWAM--GLDNSVYSQDLIEVVHACLEQDPEKRPTADEILKMPILSWRRRDMEEKVSMLNR--SNKKPRTGTVTEAPIAVVTSRSSEVYVWGGGKTTPQKLDVFKGGCRARQVCAGDAHFAVVTVEKELYTWVNMQGGSKLHGQLGHGDRASYRQPKHVEKLQGKSVQQVSCGSDFTVCISDEGQLYSFGSDYYGCLGVNQSAGAEV--LEPLLV-DFFLNEPV--EQVSCGDSHIIALTRSK-CVYSWGCGEYGRLGLDSEDDVYSPQKVEVQRDLCIVN-VCCGSDGSFLL-TLTGKVLACGLNEHNKLGLNQYTAGII---NHEAFQEVPYTTSLTLAKQLSFYKIRSISPGRTHTAAIDERGRLLTFGSNKCGQLGVGDYRKHLGINLLGGPLGGKQVIRVSCGDEFTIAATADNHIFAWGNGGNGRLAM--TPNERPQGSDICTSWPRPIFGSLHHVTDLSCRGWHTILIVEKVLNSKTIRSNSSGL 722
BLAST of NEK8-1 vs. Ensembl Xenopus
Match: nek9 (NIMA-related kinase 9 [Source:NCBI gene;Acc:100216232]) HSP 1 Score: 492.271 bits (1266), Expect = 2.112e-161 Identity = 282/704 (40.06%), Postives = 414/704 (58.81%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXX----XXXXXXXNEPTKRPSAEDVLKHPV--WKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR------CVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLG GAYG+A LYR D+SLVVWKE+ + + E + NEI ILS+L H NI+AYYNHF+ +T+ IE+EY N SL N Q+K+ A+ IH+ I+HRDIKTLNIFLT + L+K+GD+G++K L+ E A T VGT YMSPE+ QG+ YS KSDIWA GCVLYE+++ + F+ +N L+L +KI+ G + +NS ++ L +P KRP+A+++LK P+ W+ R+ P + + S +SEV+VWGGGK K++ F+ A QV AG +HF V+T EK+LYTW N+QG KL GQLGHG Y+ PK V+ L+G +++VSCG + T+C+S G+++ GS+YYG LG+N + + E + V D PV Q+SCG H A+ + +++WGCGEYGRLGL ED++Y P V++ C + V CG D +F++ + ++L CG N++NKLG+N + G+ +VP+ T + L K+ ++S G +HTA +D +L FG N GQ+G+G + H +N GK++ ++SCGD FTI +T +N IF WG +GRL + T ++R C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 34 YIPIRVLGHGAYGEATLYRRTEDDSLVVWKEVGLARLSEKERRDALNEIVILSLLQHDNIIAYYNHFLDSNTLLIELEYCNGKSLQN---QIKD----------------AVSCIHRAGILHRDIKTLNIFLTKANLIKLGDYGLAKQLSSEYSMAETCVGTLYYMSPELCQGVKYSFKSDIWAVGCVLYELLTLTRTFDATNPLNLCVKIVQGNWAM--GLDNSVYSQDLIEVVHACLEQDPEKRPTADEILKMPILSWR-----------RSLNIFYLPSPIA------VVTSRSSEVYVWGGGKTTPQKLDVFKGGCRARQVCAGDAHFAVVTVEKELYTWVNMQGGSKLHGQLGHGDRASYRQPKHVEKLQGKSVQQVSCGSDFTVCISDEGQLYSFGSDYYGCLGVNQSAGAEV--LEPLLV-DFFLNEPV--EQVSCGDSHIIALTRSK-CVYSWGCGEYGRLGLDSEDDVYSPQKVEVQRDLCIVN-VCCGSDGSFLL-TLTGKVLACGLNEHNKLGLNQYTAGIINHEAFQ---EVPYTTSLTLAKQLSFYKIRSISPGRTHTAAIDERGRLLTFGSNKCGQLGVGDYRKHLGINLLGGPLGGKQVIRVSCGDEFTIAATADNHIFAWGNGGNGRLAM--TPNERPQGSDICTSWPRPIFGSLHHVTDLSCRGWHTILIVEKVLNSKTIRSNSSGL 686
BLAST of NEK8-1 vs. Ensembl Xenopus
Match: nek9 (NIMA-related kinase 9 [Source:NCBI gene;Acc:100216232]) HSP 1 Score: 492.271 bits (1266), Expect = 7.717e-161 Identity = 282/704 (40.06%), Postives = 414/704 (58.81%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXX----XNEPTKRPSAEDVLKHPV--WKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR------CVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLG GAYG+A LYR D+SLVVWKE+ + + E + NEI ILS+L H NI+AYYNHF+ +T+ IE+EY N SL N Q+K+ A+ IH+ I+HRDIKTLNIFLT + L+K+GD+G++K L+ E A T VGT YMSPE+ QG+ YS KSDIWA GCVLYE+++ + F+ +N L+L +KI+ G + +NS L ++ +P KRP+A+++LK P+ W+ R+ P + + S +SEV+VWGGGK K++ F+ A QV AG +HF V+T EK+LYTW N+QG KL GQLGHG Y+ PK V+ L+G +++VSCG + T+C+S G+++ GS+YYG LG+N + + E + V D PV Q+SCG H A+ + +++WGCGEYGRLGL ED++Y P V++ C + V CG D +F++ + ++L CG N++NKLG+N + G+ +VP+ T + L K+ ++S G +HTA +D +L FG N GQ+G+G + H +N GK++ ++SCGD FTI +T +N IF WG +GRL + T ++R C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 34 YIPIRVLGHGAYGEATLYRRTEDDSLVVWKEVGLARLSEKERRDALNEIVILSLLQHDNIIAYYNHFLDSNTLLIELEYCNGKSLQN---QIKD----------------AVSCIHRAGILHRDIKTLNIFLTKANLIKLGDYGLAKQLSSEYSMAETCVGTLYYMSPELCQGVKYSFKSDIWAVGCVLYELLTLTRTFDATNPLNLCVKIVQGNWAM--GLDNSVYSQDLIEVVHACLEQDPEKRPTADEILKMPILSWR-----------RSLNIFYLPSPIA------VVTSRSSEVYVWGGGKTTPQKLDVFKGGCRARQVCAGDAHFAVVTVEKELYTWVNMQGGSKLHGQLGHGDRASYRQPKHVEKLQGKSVQQVSCGSDFTVCISDEGQLYSFGSDYYGCLGVNQSAGAEV--LEPLLV-DFFLNEPV--EQVSCGDSHIIALTRSK-CVYSWGCGEYGRLGLDSEDDVYSPQKVEVQRDLCIVN-VCCGSDGSFLL-TLTGKVLACGLNEHNKLGLNQYTAGIINHEAFQ---EVPYTTSLTLAKQLSFYKIRSISPGRTHTAAIDERGRLLTFGSNKCGQLGVGDYRKHLGINLLGGPLGGKQVIRVSCGDEFTIAATADNHIFAWGNGGNGRLAM--TPNERPQGSDICTSWPRPIFGSLHHVTDLSCRGWHTILIVEKVLNSKTIRSNSSGL 686
BLAST of NEK8-1 vs. Ensembl Xenopus
Match: nek9 (NIMA-related kinase 9 [Source:NCBI gene;Acc:100216232]) HSP 1 Score: 478.404 bits (1230), Expect = 6.077e-155 Identity = 280/705 (39.72%), Postives = 412/705 (58.44%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXX----XNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV---SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR------CVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLG GAYG+A LYR D+SLVVWKE+ + + E + NEI ILS+L H NI+AYYNHF+ +T+ IE+EY N SL N Q+K+ A+ IH+ I+HRDIKTLNIFLT + L+K+GD+G++K L+ E A T VGT YMSPE+ QG+ YS KSDIWA GCVLYE+++ + F+ +N L+L +KI+ G + +NS L ++ +P KRP+A+++LK P+ + M ++V N+ SN +P +G E I S +SEV+VWGGGK K++ F+ A QV AG +HF V+T EK+LYTW N+QG KL GQLGHG Y+ PK V+ L+G +++VSCG + T+C+S G+++ GS+YYG LG+N + + E + V D PV Q+SCG H A+ + +++WGCGEY L V++ C + V CG D +F++ + ++L CG N++NKLG+N + G+ +VP+ T + L K+ ++S G +HTA +D +L FG N GQ+G+G + H +N GK++ ++SCGD FTI +T +N IF WG +GRL + T ++R C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 48 YIPIRVLGHGAYGEATLYRRTEDDSLVVWKEVGLARLSEKERRDALNEIVILSLLQHDNIIAYYNHFLDSNTLLIELEYCNGKSLQN---QIKD----------------AVSCIHRAGILHRDIKTLNIFLTKANLIKLGDYGLAKQLSSEYSMAETCVGTLYYMSPELCQGVKYSFKSDIWAVGCVLYELLTLTRTFDATNPLNLCVKIVQGNWAM--GLDNSVYSQDLIEVVHACLEQDPEKRPTADEILKMPILSWRRRDMEEKVSMLNR--SNKKPRTGTVTEAPIAVVTSRSSEVYVWGGGKTTPQKLDVFKGGCRARQVCAGDAHFAVVTVEKELYTWVNMQGGSKLHGQLGHGDRASYRQPKHVEKLQGKSVQQVSCGSDFTVCISDEGQLYSFGSDYYGCLGVNQSAGAEV--LEPLLV-DFFLNEPV--EQVSCGDSHIIALTRSK-CVYSWGCGEYVSL--------LSFWQVEVQRDLCIVN-VCCGSDGSFLL-TLTGKVLACGLNEHNKLGLNQYTAGII---NHEAFQEVPYTTSLTLAKQLSFYKIRSISPGRTHTAAIDERGRLLTFGSNKCGQLGVGDYRKHLGINLLGGPLGGKQVIRVSCGDEFTIAATADNHIFAWGNGGNGRLAM--TPNERPQGSDICTSWPRPIFGSLHHVTDLSCRGWHTILIVEKVLNSKTIRSNSSGL 708
BLAST of NEK8-1 vs. Ensembl Mouse
Match: Nek9 (NIMA (never in mitosis gene a)-related expressed kinase 9 [Source:MGI Symbol;Acc:MGI:2387995]) HSP 1 Score: 515.768 bits (1327), Expect = 8.215e-169 Identity = 286/702 (40.74%), Postives = 434/702 (61.82%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSL-SNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIING--EFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV-SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINS-NETEMINQFE-KITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR----CVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLG+GA+G+A LYR D+SLVVWKE+D+ + E + NEI IL++L H NI+AYYNHF+ T+ IE+EY N G+L LRQ + E V+WY FQ++ A+ IHK I+HRDIKTLNIFLT + L+K+GD+G++K LN E A T VGTP YMSPE+ QG+ Y+ KSDIWA GCV++E+++ K+ F+ +N L+L +KI+ G + + + +L+ L+ L +P +RP+A+ +L P+ + + M ++V N R + ++V S SEV+VWGGGK K++ + SA QV AG +HF V+T EK+LYTW N+QG KL GQLGHG Y+ PK V+ L+G I +VSCG++ T+C++ G+++ GS+YYG +G++ + E++ + +++P++ Q+SCG +H + +++ ++WGCGEYGRLGL E++ Y P VD+P + V CG D TF++ + S ++L CG N++NKLG+N Q +SG ++VP+ T F + L K+ T++ G +HTA +D +L FG N GQ+G+G+ K +N GK++ ++SCGD FTI +T +N IF WG +GRL + T C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 52 YIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQLVHACLDQDPEQRPAADALLDLPLLRTRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIHQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVSGPEVLEPMQLNFFLSNPVE-------QVSCGDNHVVVLTRNKEV-YSWGCGEYGRLGLDSEEDYYTPQRVDVP-KALIIVAVQCGCDGTFLLTQ-SGKVLACGLNEFNKLGLN---QCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLGVGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGL 740
BLAST of NEK8-1 vs. Ensembl Mouse
Match: Nek8 (NIMA (never in mitosis gene a)-related expressed kinase 8 [Source:MGI Symbol;Acc:MGI:1890646]) HSP 1 Score: 294.664 bits (753), Expect = 6.948e-88 Identity = 217/673 (32.24%), Postives = 339/673 (50.37%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDID---KNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVH------RTNQANSNSRP----CSGDRKERS----------------IVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYKIPKLVQSL----KGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIV--AVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKV--FAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLG 2037
Y ++RV+G+GA+G L D LV+ K+I ++ K + QNE +L +L+HPN++ YY +F+ + I MEYA G+L+ ++ ++ L E +L +F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L+ A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ LL EP +RP ++ P+ C + +H R +A + P SG R+ I S V+ WGGG + L++ + +QVAAG + +T L W G L GA+ P+ V GV IK V+CG+ T CL+ G + GS G LG + N T++ + T+ + + ++ Q++CG H A+ + LF WG G+ GRLGLG ++ P V + + + + V CG D++ I+ R+L CG+N++NKLG++ LS + V F + A L + V G +H+A + Y FG N +GQ+G T + + +V G K+ ++CGD FT+ E++ WG+ GRLG
Sbjct: 4 YERIRVVGRGAFGIVHLCLRKADQKLVILKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPL----CIRALLNIHTDVGSVRMRRAEKSLTPGPPIASGSTGSRATSARCRGVPRGPVRPAIPPPLSSVYAWGGGLSSPLRLPMLNTE--VVQVAAGRTQKAGVTRSGRLILWEAPPLGAG-GGTLLPGAVELPQPQFVSRFLEGQSGVTIKHVACGDLFTACLTDRGIIMTFGSGSNGCLG-HGNLTDI----SQPTIVEALLGYEMV--QVACGASHVLALS-TDGELFAWGRGDGGRLGLGTRESHNCPQQVPVAPGQ-EAQRVVCGIDSSMIL-TSPGRVLACGSNRFNKLGLD----HLSLDEEPVPYQQVEEALSFTPLGSAPLDQEPLLCVDLGTAHSAAITASGDCYTFGSNQHGQLG---TSSRRVSRAPCRVQGLEGIKMVMVACGDAFTVAVGAEGEVYSWGKGTRGRLG 652
BLAST of NEK8-1 vs. Ensembl Mouse
Match: Nek4 (NIMA (never in mitosis gene a)-related expressed kinase 4 [Source:MGI Symbol;Acc:MGI:1344404]) HSP 1 Score: 177.563 bits (449), Expect = 1.127e-49 Identity = 98/259 (37.84%), Postives = 155/259 (59.85%), Query Frame = 1
Query: 130 VSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINE---IQNEIDILSILDHPNIVAYYNHFVYID-TVFIEMEYANAGSLSNYLRQLK-EDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVL-NEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWK 888
+Y +RV+G+G+YG+ L ++ D V K++++ E + E +LS L HPNIV Y + D ++I M + G L L++ K + L ES V+ +F Q+ AL Y+H+ HI+HRD+KT N+FLT + ++K+GD GI++VL N + A+T +GTP YMSPE+ PY+ KSD+WA GC +YE+ + K F + SL +II G+ MP +++L +L++ +L P +RPS +L+ P K
Sbjct: 4 AAYCYMRVVGRGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIK 262
BLAST of NEK8-1 vs. Ensembl Mouse
Match: Nek4 (NIMA (never in mitosis gene a)-related expressed kinase 4 [Source:MGI Symbol;Acc:MGI:1344404]) HSP 1 Score: 178.718 bits (452), Expect = 2.742e-46 Identity = 107/298 (35.91%), Postives = 171/298 (57.38%), Query Frame = 1
Query: 130 VSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINE---IQNEIDILSILDHPNIVAYYNHFVYID-TVFIEMEYANAGSLSNYLRQLK-EDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVL-NEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMID----QVHRTNQANSNSR--PCSG--DRKERS 981
+Y +RV+G+G+YG+ L ++ D V K++++ E + E +LS L HPNIV Y + D ++I M + G L L++ K + L ES V+ +F Q+ AL Y+H+ HI+HRD+KT N+FLT + ++K+GD GI++VL N + A+T +GTP YMSPE+ PY+ KSD+WA GC +YE+ + K F + SL +II G+ MP +++L +L++ +L P +RPS +L+ P K + ++ + + N N +SR P + RKE S
Sbjct: 4 AAYCYMRVVGRGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIKHHISLFLEATKAKTSKNNVKNCDSRAKPVAAVVSRKEES 301
BLAST of NEK8-1 vs. Ensembl Mouse
Match: Nek4 (NIMA (never in mitosis gene a)-related expressed kinase 4 [Source:MGI Symbol;Acc:MGI:1344404]) HSP 1 Score: 178.333 bits (451), Expect = 5.408e-46 Identity = 98/259 (37.84%), Postives = 155/259 (59.85%), Query Frame = 1
Query: 130 VSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINE---IQNEIDILSILDHPNIVAYYNHFVYID-TVFIEMEYANAGSLSNYLRQLK-EDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVL-NEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWK 888
+Y +RV+G+G+YG+ L ++ D V K++++ E + E +LS L HPNIV Y + D ++I M + G L L++ K + L ES V+ +F Q+ AL Y+H+ HI+HRD+KT N+FLT + ++K+GD GI++VL N + A+T +GTP YMSPE+ PY+ KSD+WA GC +YE+ + K F + SL +II G+ MP +++L +L++ +L P +RPS +L+ P K
Sbjct: 4 AAYCYMRVVGRGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKESWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPESQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLTRTNIIKVGDLGIARVLENHGDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKDMNSLVYRIIEGKLPPMPKVYSTELAELIRTMLSRRPEERPSVRSILRQPYIK 262
BLAST of NEK8-1 vs. UniProt/SwissProt
Match: sp|Q8TD19|NEK9_HUMAN (Serine/threonine-protein kinase Nek9 OS=Homo sapiens OX=9606 GN=NEK9 PE=1 SV=2) HSP 1 Score: 533.487 bits (1373), Expect = 9.438e-175 Identity = 285/702 (40.60%), Postives = 435/702 (61.97%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSL-SNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIING--EFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV-SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINS-NETEMINQFE-KITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR----CVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLG+GA+G+A LYR D+SLVVWKE+D+ + E + NEI IL++L H NI+AYYNHF+ T+ IE+EY N G+L LRQ + E V+WY FQ++ A+ IHK I+HRDIKTLNIFLT + L+K+GD+G++K LN E A T VGTP YMSPE+ QG+ Y+ KSDIWA GCV++E+++ K+ F+ +N L+L +KI+ G + + + +L+ ++ L +P +RP+A+++L P+ + + M ++V N R + ++V S SEV+VWGGGK K++ + SA QV AG +HF V+T EK+LYTW N+QG KL GQLGHG Y+ PK V+ L+G I++VSCG++ T+C++ G+++ GS+YYG +G++ E++ + +++P++ Q+SCG +H + +++ ++WGCGEYGRLGL E++ Y P VD+P + V CG D TF++ + S ++L CG N++NKLG+N Q +SG ++VP+ T F + L K+ T++ G +HTA +D +L FG N GQ+G+G+ K +N GK++ ++SCGD FTI +T +N IF WG +GRL + T C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 52 YIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQMVHSCLDQDPEQRPTADELLDRPLLRKRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIRQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVAGPEVLEPMQLNFFLSNPVE-------QVSCGDNHVVVLTRNKEV-YSWGCGEYGRLGLDSEEDYYTPQKVDVP-KALIIVAVQCGCDGTFLLTQ-SGKVLACGLNEFNKLGLN---QCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLGVGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGL 740
BLAST of NEK8-1 vs. UniProt/SwissProt
Match: sp|Q7ZZC8|NEK9_XENLA (Serine/threonine-protein kinase Nek9 OS=Xenopus laevis OX=8355 GN=nek9 PE=2 SV=1) HSP 1 Score: 530.02 bits (1364), Expect = 6.578e-174 Identity = 291/706 (41.22%), Postives = 431/706 (61.05%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDL-SESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXX----XNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV---SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR------CVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLG GAYG+A LYR D+SLVVWKE+ + + E + NEI ILS+L H NI+AYYNHF+ +T+ IE+EY N G+L + + K L E VLWY FQ++ A+ IHK I+HRDIKTLNIFLT + L+K+GD+G++K L+ E A T VGT YMSPE+ QG+ YS KSDIWA GCVLYE+++ + F+ +N L+L +KI+ G + +N+ L ++ +P KRP+A+++L+ P+ + M ++V N+ SN +P +G E I S +SEV+VWGGGK K++ F+ A QV AG +HF V+T EK+LYTW N+QG KL GQLGHG Y+ PK V+ L+G +++VSCG + T+C++ G+++ GS+YYG LG+N + + E + V D PV +Q+SCG H A+ + + ++WGCGEYGRLGL ED++Y P V++ C + V CG D +F++ + ++L CG N++NKLG+N + G+ +VP+ T + L K+ ++S G +HTA +D +L FG N GQ+G+G + H +N GK++ ++SCGD FT +T +N IF WG +GRL + T ++R C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 34 YIPIRVLGHGAYGEATLYRRTEDDSLVVWKEVGLARLSEKERRDALNEIVILSLLQHDNIIAYYNHFLDSNTLLIELEYCNGGNLFDKIVHQKAQLFQEETVLWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKQLSSEYSMAETCVGTLYYMSPEICQGVKYSFKSDIWAVGCVLYELLTLTRTFDATNPLNLCVKIVQGNWAV--GLDNTVYTQELIEVVHACLEQDPEKRPTADEILERPILSWRRRDMEEKVSMLNR--SNKKPRTGTVTEAPIAVVTSRSSEVYVWGGGKTTPQKLDVFKGGWRARQVCAGDAHFAVVTVEKELYTWVNMQGGSKLHGQLGHGDRASYRQPKHVEKLQGKSVQKVSCGSDFTVCITDEGQLYSFGSDYYGCLGVNQSAGSEV--LEPLLV-DFFLNEPV--DQVSCGDSHIMALTRSKSV-YSWGCGEYGRLGLDSEDDVYSPQKVEVQRGLCIVN-VCCGSDGSFLL-TLTGKVLACGLNEHNKLGLNQYTAGII---NHEAFQEVPYTTSLTLAKQLSFYKIRSISPGRTHTAAIDERGRLLTFGSNKCGQLGVGDYRKHLGINLLGGPLGGKQVIRVSCGDEFTTAATADNHIFAWGNGGNGRLAM--TPNERPQGSDICTSWPRPIFGSLHHVTDLSCRGWHTILIVEKVLNSKTIRSNSSGL 722
BLAST of NEK8-1 vs. UniProt/SwissProt
Match: sp|Q8K1R7|NEK9_MOUSE (Serine/threonine-protein kinase Nek9 OS=Mus musculus OX=10090 GN=Nek9 PE=1 SV=2) HSP 1 Score: 515.768 bits (1327), Expect = 5.746e-168 Identity = 286/702 (40.74%), Postives = 434/702 (61.82%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSL-SNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIING--EFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV-SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINS-NETEMINQFE-KITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR----CVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLG+GA+G+A LYR D+SLVVWKE+D+ + E + NEI IL++L H NI+AYYNHF+ T+ IE+EY N G+L LRQ + E V+WY FQ++ A+ IHK I+HRDIKTLNIFLT + L+K+GD+G++K LN E A T VGTP YMSPE+ QG+ Y+ KSDIWA GCV++E+++ K+ F+ +N L+L +KI+ G + + + +L+ L+ L +P +RP+A+ +L P+ + + M ++V N R + ++V S SEV+VWGGGK K++ + SA QV AG +HF V+T EK+LYTW N+QG KL GQLGHG Y+ PK V+ L+G I +VSCG++ T+C++ G+++ GS+YYG +G++ + E++ + +++P++ Q+SCG +H + +++ ++WGCGEYGRLGL E++ Y P VD+P + V CG D TF++ + S ++L CG N++NKLG+N Q +SG ++VP+ T F + L K+ T++ G +HTA +D +L FG N GQ+G+G+ K +N GK++ ++SCGD FTI +T +N IF WG +GRL + T C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 52 YIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVILALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEEEMVVWYLFQIVSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYMSPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQYSLELIQLVHACLDQDPEQRPAADALLDLPLLRTRRREMEEKVTLLNAPTKRPRSSTVTEAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYTWVNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIHQVSCGDDFTVCVTDEGQLYAFGSDYYGCMGVDKVSGPEVLEPMQLNFFLSNPVE-------QVSCGDNHVVVLTRNKEV-YSWGCGEYGRLGLDSEEDYYTPQRVDVP-KALIIVAVQCGCDGTFLLTQ-SGKVLACGLNEFNKLGLN---QCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCNKCGQLGVGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRLAMTPTERPHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGL 740
BLAST of NEK8-1 vs. UniProt/SwissProt
Match: sp|Q86SG6|NEK8_HUMAN (Serine/threonine-protein kinase Nek8 OS=Homo sapiens OX=9606 GN=NEK8 PE=1 SV=1) HSP 1 Score: 324.324 bits (830), Expect = 3.077e-98 Identity = 214/665 (32.18%), Postives = 337/665 (50.68%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDID---KNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVH------RTNQANSNSRP-----------CSGDRK---ERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYKIPKLVQSL----KGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKV--FAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLG 2037
Y ++RV+G+GA+G L D LV+ K+I ++ K + QNE +L +L+HPN++ YY +F+ + I MEYA G+L+ ++ ++ L E +L +F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L+ A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ LL EP +RP ++ P+ C + +H R +A + P C G + +I S V+ WGGG L++ + +QVAAG + +T L W G L GA+ P+ + GV IK V+CG+ T CL+ G + GS G LG S + + T+ + + ++ Q++CG H A+ E LF WG G+ GRLGLG ++ P V +P + + + V CG D++ I+ + L CG+N++NKLG++ + G + + + F A L + ++ G +H+A + Y FG N +GQ+G T T + KV G K+ ++CGD FT+ +E++ WG+ GRLG
Sbjct: 4 YERIRVVGRGAFGIVHLCLRKADQKLVIIKQIPVEQMTKEERQAAQNECQVLKLLNHPNVIEYYENFLEDKALMIAMEYAPGGTLAEFIQKRCNSLLEEETILHFFVQILLALHHVHTHLILHRDLKTQNILLDKHRMVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLVLSLLSLEPAQRPPLSHIMAQPL----CIRALLNLHTDVGSVRMRRAEKSVAPSNTGSRTTSVRCRGIPRGPVRPAIPPPLSSVYAWGGGLGTPLRLPMLNTE--VVQVAAGRTQKAGVTRSGRLILWEAPPLGAG-GGSLLPGAVEQPQPQFISRFLEGQSGVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHGS-----LTDISQPTIVEALLGYEMV--QVACGASHVLAL-STERELFAWGRGDSGRLGLGTRESHSCPQQVPMPPGQ-EAQRVVCGIDSSMIL-TVPGQALACGSNRFNKLGLDHLSLGEEPVPHQQVEEALSF--TLLGSAPLDQEPLLSIDLGTAHSAAVTASGDCYTFGSNQHGQLG---TNTRRGSRAPCKVQGLEGIKMAMVACGDAFTVAIGAESEVYSWGKGARGRLG 646 HSP 2 Score: 58.151 bits (139), Expect = 2.578e-7 Identity = 33/99 (33.33%), Postives = 51/99 (51.52%), Query Frame = 1
Query: 1774 NVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFA--GKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRC 2064
V + V+CG+ TA L + FG S G +G G + D+++ + V A G ++ +++CG + +T E+F WGR DSGRLGL C
Sbjct: 390 GVTIKHVACGDFFTACLTDRGIIMTFGSGSNGCLGHG---SLTDISQPTIVEALLGYEMVQVACGASHVLALSTERELFAWGRGDSGRLGLGTRESHSC 485
BLAST of NEK8-1 vs. UniProt/SwissProt
Match: sp|Q90XC2|NEK8_DANRE (Serine/threonine-protein kinase Nek8 OS=Danio rerio OX=7955 GN=nek8 PE=2 SV=1) HSP 1 Score: 320.857 bits (821), Expect = 8.068e-97 Identity = 219/669 (32.74%), Postives = 348/669 (52.02%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINE---IQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKH-----PVWK-----GNCQ-SMIDQVHRTNQANSNSRP---CSGDRKERSIVSLNS----------EVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTW---SNIQGDKKLSGQLGHGAIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGI-NSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIV--AVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLG 2037
Y + +V+G+GA+G L R D++LV+ KEI +++ +E QNE +L +L HPNI+ YY +F+ + I MEYA G+L++Y+ ++ L E +L F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ +L +P+KRP +++ H P+ GN + I++ QA + RP + R + SL S V+ WG G L++ + +QV+ G + + +T L TW S G+ L G + + I + ++ GV IK VSCG+ T CL+ G + GS G LG N N+ E + + + Q+SCG H A+ E +F+WG G+ GRLGL +D+ P V +P + + V CG D + I+ ++L CGNN++NKLG++ +SG+ + + V F++V A L K+ + G +H+ + + + FG N +GQ+G H ++ + + S + + I +CGD FT+ E++ WG+ GRLG
Sbjct: 4 YEKTKVVGRGAFGIVHLCRRRTDSALVILKEIPVEQMTRDERLAAQNECQVLKLLSHPNIIEYYENFLEDKALMIAMEYAPGGTLADYIQKRCNSLLDEDTILHSFVQILLALYHVHNKLILHRDLKTQNILLDKHQMIVKIGDFGISKILVSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLILNMLNLDPSKRPQLNEIMAHAICIRPLLNLYTDIGNVKMRRIEKPLSNVQAGPHGRPGGWITSTRTRGGLSSLTSSKMMHPLPLFSVYTWGSGISTPLRLPMLNTE--VIQVSLGRTQKMGVTKSGRLITWEAPSVGSGEPTLPGAVEQMQPQF-ISRFLEGQSGVTIKSVSCGDLFTTCLTDRGIIMTFGSGSNGCLGHGNFNDVTQPKIVEALLGYELV--------QVSCGASHVLAVTN-EREVFSWGRGDNGRLGLATQDSHNCPQQVSLPA-DFEAQRVLCGVDCSMIM-STQHQILACGNNRFNKLGLD----KVSGTEEPSSFCQVEEVHLFQLVQSAPLNTEKIVYIDIGTAHSVAVTEKGQCFTFGSNQHGQLGCSHRRSSRVPYQVSGL---QGITMAACGDAFTLAIGAEGEVYTWGKGARGRLG 651 HSP 2 Score: 57.3806 bits (137), Expect = 5.044e-7 Identity = 34/98 (34.69%), Postives = 48/98 (48.98%), Query Frame = 1
Query: 1777 VKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFA--GKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRC 2064
V + +VSCG+ T L + FG S G +G G+ DV + V A G ++ ++SCG + T E+F WGR D+GRLGL C
Sbjct: 398 VTIKSVSCGDLFTTCLTDRGIIMTFGSGSNGCLGHGN---FNDVTQPKIVEALLGYELVQVSCGASHVLAVTNEREVFSWGRGDNGRLGLATQDSHNC 492
BLAST of NEK8-1 vs. TrEMBL
Match: A0A0H3YFE5 (NEK8-1 OS=Schmidtea mediterranea OX=79327 GN=NEK8-1 PE=2 SV=1) HSP 1 Score: 1603.19 bits (4150), Expect = 0.000e+0 Identity = 803/803 (100.00%), Postives = 803/803 (100.00%), Query Frame = 1
Query: 67 MDNCRDNEVLSNRDFSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRCVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAIMDKLSLKNVNSFETEYDKDGSGDIQTWLRKELQEDVIYMDQVSVNLHXXXXXXXXXXXXXELYSCECGCQKCQNYIKFLENRIRLLCDKNILQ 2475
MDNCRDNEVLSNRDFSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSDLLDLLQRLLLNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRCVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAIMDKLSLKNVNSFETEYDKDGSGDIQTWLRKELQEDVIYMDQVSVNLHSINVNSYSSNNSNELYSCECGCQKCQNYIKFLENRIRLLCDKNILQ
Sbjct: 1 MDNCRDNEVLSNRDFSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSDLLDLLQRLLLNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRCVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAIMDKLSLKNVNSFETEYDKDGSGDIQTWLRKELQEDVIYMDQVSVNLHSINVNSYSSNNSNELYSCECGCQKCQNYIKFLENRIRLLCDKNILQ 803
BLAST of NEK8-1 vs. TrEMBL
Match: A0A1I8HFL4 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 577.4 bits (1487), Expect = 0.000e+0 Identity = 303/704 (43.04%), Postives = 432/704 (61.36%), Query Frame = 1
Query: 133 SYVQVRVLGKGAYGKAVLYRNAVDNS--LVVWKEIDI---DKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGE--FQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV-SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIIN----QISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEI----------TVDQRCVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTI 2175
SY+ + LGKGA+G+A LYR +N LVVWK I++ D+ + E QNEID+L +LDHPNIVAYYNHF + + IEMEYAN GSL ++ Q L E ++WYFFQ+ A+ +IH+ I+HRDIKTLNIFLT + L+K+GDFGI+KVL DE A T GTP YMSPE++QG Y+ KSDIWA GCVL+E+++ K+ F+ +N L LA I++ E + + L LL L EP RPSA ++L + ++ + + M +V Q + +RP S +V S +EVF WGGGK +KIEQF+ ++SALQVA G H V+T E++++TW N+QG KL GQLGHG + Y+ PK V+ L GV I+E CG+E T+ + G+++ CGSNY+G LG+ + E + +P+ P +N +++CG H A+ + +F WGCGE+GRLGL EDN P V +P+ + + CG D +F++ R+L CG+N++NKLG N A+GL+ ++ +D P FK+V L +V +SCG SHTA +D N+L +FG N G++GLG + V + GK+ +++CGD FT+ TT+N I+ WGRAD+GRLG+E+ + CV PR +FG+L ADI S W ++MI ER+LE T+
Sbjct: 285 SYIPINCLGKGAFGEAWLYRKTEENEEQLVVWKMINLTGLDERRRREAQNEIDVLHMLDHPNIVAYYNHFFDGNILLIEMEYANGGSLYQFIGQCNSLLKEETIIWYFFQMTMAIRHIHEASILHRDIKTLNIFLTKAGLIKLGDFGIAKVLGSDEMAETCQGTPYYMSPELVQGKRYNYKSDIWALGCVLFELLTLKRAFDATNQLKLAQNIVHTEISLSLVDESYSVQLKALLGATLSKEPEARPSASEILINKLFS-DGERMQSRVRELQQQSRLARPSSAGTDTTPVVTSKTTEVFYWGGGKQTPIKIEQFERQNSALQVATGSHHMAVVTIEREVFTWCNVQGCAKLCGQLGHGDLASYRTPKKVEKLNGVPIEEARCGDEFTVLRTVEGQLYACGSNYHGCLGVPREQLEE-ETGDSDQALEPV-PVPFFLNLEVKRVACGDAHVLAVTR-QSEVFAWGCGEFGRLGLNGEDNYDTPQPVSLPVKRQLIVGACCGSDGSFLLTD-GGRVLACGSNEHNKLGFNSVAKGLT-AANSTCSFDTPCVHTFKVVKPLTKYRVRQLSCGRSHTAAIDDCNQLLMFGSNRMGELGLGEMRHRNGVFNIGRACGGKRFLRVACGDGFTVACTTDNHIYSWGRADNGRLGIELQAASLLNMNKALASACVSTPRPMFGSLHLVADISSRHWHSLMIAERVLEQRTV 982
BLAST of NEK8-1 vs. TrEMBL
Match: A0A267F6Z2 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022512g2 PE=4 SV=1) HSP 1 Score: 576.63 bits (1485), Expect = 0.000e+0 Identity = 303/704 (43.04%), Postives = 432/704 (61.36%), Query Frame = 1
Query: 133 SYVQVRVLGKGAYGKAVLYRNAVDNS--LVVWKEIDI---DKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGE--FQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV-SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIIN----QISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEI----------TVDQRCVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTI 2175
SY+ + LGKGA+G+A LYR +N LVVWK I++ D+ + E QNEID+L +LDHPNIVAYYNHF + + IEMEYAN GSL ++ Q L E ++WYFFQ+ A+ +IH+ I+HRDIKTLNIFLT + L+K+GDFGI+KVL DE A T GTP YMSPE++QG Y+ KSDIWA GCVL+E+++ K+ F+ +N L LA I++ E + + L LL L EP RPSA ++L + ++ + + M +V Q + +RP S +V S +EVF WGGGK +KIEQF+ ++SALQVA G H V+T E++++TW N+QG KL GQLGHG + Y+ PK V+ L GV I+E CG+E T+ + G+++ CGSNY+G LG+ + E + +P+ P +N +++CG H A+ + +F WGCGE+GRLGL EDN P V +P+ + + CG D +F++ R+L CG+N++NKLG N A+GL+ ++ +D P FK+V L +V +SCG SHTA +D N+L +FG N G++GLG + V + GK+ +++CGD FT+ TT+N I+ WGRAD+GRLG+E+ + CV PR +FG+L ADI S W ++MI ER+LE T+
Sbjct: 28 SYIPINCLGKGAFGEAWLYRKTEENEEQLVVWKMINLTGLDERRRREAQNEIDVLHMLDHPNIVAYYNHFFDGNILLIEMEYANGGSLYQFIGQCNSLLKEETIIWYFFQMTMAIRHIHEASILHRDIKTLNIFLTKAGLIKLGDFGIAKVLGSDEMAETCQGTPYYMSPELVQGKRYNYKSDIWALGCVLFELLTLKRAFDATNQLKLAQNIVHTEISLSLVDESYSVQLKALLGATLSKEPEARPSASEILINKLFS-DGERMQSRVRELQQQSRLARPSSAGTDTTPVVTSKTTEVFYWGGGKQTPIKIEQFERQNSALQVATGSHHMAVVTIEREVFTWCNVQGCAKLCGQLGHGDLASYRTPKKVEKLNGVPIEEARCGDEFTVLRTVEGQLYACGSNYHGYLGVPREQLEE-ETGDSDQALEPV-PVPFFLNLEVKRVACGDAHVLAVTR-QSEVFAWGCGEFGRLGLNGEDNYDTPQPVSLPVKRQLIVGACCGSDGSFLLTD-GGRVLACGSNEHNKLGFNSVAKGLT-AANSTCSFDTPCVHTFKVVKPLTKYRVRQLSCGRSHTAAIDDCNQLLMFGSNRMGELGLGEMRHRNGVFNIGRACGGKRFLRVACGDGFTVACTTDNHIYSWGRADNGRLGIELQAASLLNMNKALASACVSTPRPMFGSLHLVADISSRHWHSLMIAERVLEQRTV 725
BLAST of NEK8-1 vs. TrEMBL
Match: A0A267EN97 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022512g3 PE=4 SV=1) HSP 1 Score: 575.859 bits (1483), Expect = 0.000e+0 Identity = 303/704 (43.04%), Postives = 431/704 (61.22%), Query Frame = 1
Query: 133 SYVQVRVLGKGAYGKAVLYRNAVDNS--LVVWKEIDI---DKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGE--FQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV-SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIIN----QISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITV----------DQRCVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTI 2175
SY+ + LGKGA+G+A LYR +N LVVWK I++ D+ + E QNEID+L +LDHPNIVAYYNHF + + IEMEYAN GSL ++ Q L E ++WYFFQ+ A+ +IH+ I+HRDIKTLNIFLT + L+K+GDFGI+KVL DE A T GTP YMSPE++QG Y+ KSDIWA GCVL+E+++ K+ F+ +N L LA I++ E + + L LL L EP RPSA ++L + ++ + + M +V Q + +RP S +V S +EVF WGGGK +KIEQF+ ++SALQVA G H V+T E++++TW N+QG KL GQLGHG + Y+ PK V+ L GV I+E CG+E T+ + G+++ CGSNY+G LG+ + E + +P+ P +N +++CG H A+ + +F WGCGE+GRLGL EDN P V +P+ + + CG D +F++ R+L CG+N++NKLG N A+GL+ ++ +D P FK+V L +V +SCG SHTA +D N+L +FG N G++GLG + V + GK+ +++CGD FT+ TT+N I+ WGRAD+GRLG+E+ CV PR +FG+L ADI S W ++MI ER+LE T+
Sbjct: 28 SYIPINCLGKGAFGEAWLYRKTEENEEQLVVWKMINLTGLDERRRREAQNEIDVLHMLDHPNIVAYYNHFFDGNILLIEMEYANGGSLYQFIGQCNSLLKEETIIWYFFQMTMAIRHIHEASILHRDIKTLNIFLTKAGLIKLGDFGIAKVLGSDEMAETCQGTPYYMSPELVQGKRYNYKSDIWALGCVLFELLTLKRAFDATNQLKLAQNIVHTEISLSLVDESYSVQLKALLGATLSKEPEARPSASEILINKLFS-DGERMQSRVRELQQQSRLARPSSAGTDTTPVVTSKTTEVFYWGGGKQTPIKIEQFERQNSALQVATGSHHMAVVTIEREVFTWCNVQGCAKLCGQLGHGDLASYRTPKKVEKLNGVPIEEARCGDEFTVLRTVEGQLYACGSNYHGCLGVPREQLEE-ETGDSDQALEPV-PVPFFLNLEVKRVACGDAHVLAVTR-QSEVFAWGCGEFGRLGLNGEDNYDTPQPVSLPVKRQLIVGACCGSDGSFLLTD-GGRVLACGSNEHNKLGFNSVAKGLT-AANSTCSFDTPCVHTFKVVKPLTKYRVRQLSCGRSHTAAIDDCNQLLMFGSNRMGELGLGEMRHRNGVFNIGRACGGKRFLRVACGDGFTVACTTDNHIYSWGRADNGRLGIELQAASLLNMNKASASACVSTPRPMFGSLHLVADISSRHWHSLMIAERVLEQRTV 725
BLAST of NEK8-1 vs. TrEMBL
Match: A0A1I8HNF1 (Protein kinase domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig022512g1 PE=4 SV=1) HSP 1 Score: 575.859 bits (1483), Expect = 0.000e+0 Identity = 303/704 (43.04%), Postives = 431/704 (61.22%), Query Frame = 1
Query: 133 SYVQVRVLGKGAYGKAVLYRNAVDNS--LVVWKEIDI---DKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGE--FQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV-SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIIN----QISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITV----------DQRCVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTI 2175
SY+ + LGKGA+G+A LYR +N LVVWK I++ D+ + E QNEID+L +LDHPNIVAYYNHF + + IEMEYAN GSL ++ Q L E ++WYFFQ+ A+ +IH+ I+HRDIKTLNIFLT + L+K+GDFGI+KVL DE A T GTP YMSPE++QG Y+ KSDIWA GCVL+E+++ K+ F+ +N L LA I++ E + + L LL L EP RPSA ++L + ++ + + M +V Q + +RP S +V S +EVF WGGGK +KIEQF+ ++SALQVA G H V+T E++++TW N+QG KL GQLGHG + Y+ PK V+ L GV I+E CG+E T+ + G+++ CGSNY+G LG+ + E + +P+ P +N +++CG H A+ + +F WGCGE+GRLGL EDN P V +P+ + + CG D +F++ R+L CG+N++NKLG N A+GL+ ++ +D P FK+V L +V +SCG SHTA +D N+L +FG N G++GLG + V + GK+ +++CGD FT+ TT+N I+ WGRAD+GRLG+E+ CV PR +FG+L ADI S W ++MI ER+LE T+
Sbjct: 28 SYIPINCLGKGAFGEAWLYRKTEENEEQLVVWKMINLTGLDERRRREAQNEIDVLHMLDHPNIVAYYNHFFDGNILLIEMEYANGGSLYQFIGQCNSLLKEETIIWYFFQMTMAIRHIHEASILHRDIKTLNIFLTKAGLIKLGDFGIAKVLGSDEMAETCQGTPYYMSPELVQGKRYNYKSDIWALGCVLFELLTLKRAFDATNQLKLAQNIVHTEISLSLVDESYSVQLKALLGATLSKEPEARPSASEILINKLFS-DGERMQSRVRELQQQSRLARPSSAGTDTTPVVTSKTTEVFYWGGGKQTPIKIEQFERQNSALQVATGSHHMAVVTIEREVFTWCNVQGCAKLCGQLGHGDLASYRTPKKVEKLNGVPIEEARCGDEFTVLRTVEGQLYACGSNYHGCLGVPREQLEE-ETGDSDQALEPV-PVPFFLNLEVKRVACGDAHVLAVTR-QSEVFAWGCGEFGRLGLNGEDNYDTPQPVSLPVKRQLIVGACCGSDGSFLLTD-GGRVLACGSNEHNKLGFNSVAKGLT-AANSTCSFDTPCVHTFKVVKPLTKYRVRQLSCGRSHTAAIDDCNQLLMFGSNRMGELGLGEMRHRNGVFNIGRACGGKRFLRVACGDGFTVACTTDNHIYSWGRADNGRLGIELQAASLLNMNKASASACVSTPRPMFGSLHLVADISSRHWHSLMIAERVLEQRTV 725
BLAST of NEK8-1 vs. Ensembl Cavefish
Match: NEK9 (NIMA related kinase 9 [Source:NCBI gene;Acc:103037873]) HSP 1 Score: 547.354 bits (1409), Expect = 0.000e+0 Identity = 292/701 (41.65%), Postives = 439/701 (62.62%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDK---NKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDL-SESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCN--NSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNS-EVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQ--KNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITV---DQRCVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +RVLGKGA+G+A LYR DNSLVVWKEID++ K ++ NEI ILSIL H NI+AY+NHF+ +T+ IE+EY N G+L + + Q K L +E V+WY +Q+ A+ +IHK ++HRDIKTLNIFLT + L+K+GD+G++K L+ E A T VGTP YMSPE+ QG+ Y+ KSDIWA GCVLYE+++ + F+ +N L+L +KI+ G + N + +S+L+ ++ L +P KRP+++ +L P+ Q + D+V N A + + +IV+ S EV+ WGGGK K++ F+ +SA QV +G +HF V+T EK+LYTW+++QG K+ GQLGHG Y+ P+ V+ L+G I++V+CG + T C++ +M+ GS+YYG +G+ E EM + + + + ++ PV Q+SCG +H + D+ ++WGCGE+GRLGL ED+ P LV++P + VYCG D TF + + S ++L CGNN NKLG+N QG++G + ++P+ T +V L K+ ++ G +HTA +D +L FG N YGQ+G+ K H+ V F GK + K+SCGD FTI +T +N+IF WG A +GRLG+ + C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 39 YIPIRVLGKGAFGEATLYRRTEDNSLVVWKEIDLNNLSDKKRRDVMNEISILSILQHHNIIAYFNHFMDKNTLLIELEYCNGGNLYDKIIQQKGKLFTEEIVIWYLYQIASAVAHIHKAGVLHRDIKTLNIFLTKTNLIKLGDYGLAKKLDSEYSMAETCVGTPYYMSPELCQGVKYNFKSDIWAMGCVLYELLTLTRTFDATNPLNLCVKIVQGNWTMEVNSDVYSSELIKIVYACLDQDPEKRPTSDQILDQPIISRVRQELEDRVAVLNSAIRKPKLSTVSETPVAIVTTRSREVYFWGGGKFTPQKLDMFKGGNSAQQVCSGETHFAVVTVEKELYTWASVQGGAKMVGQLGHGDQASYRQPRKVERLQGKAIRQVACGADFTACVTDEDQMYMFGSDYYGCIGV---ENEMGMEVLEPVLLEFFEERPV--RQVSCGDNHVVVLTHSGDI-YSWGCGEHGRLGLDCEDDFASPMLVEVP-KGASIDSVYCGSDGTFFLTE-SGKVLACGNNDLNKLGLN---QGITGIKNHFREGSQEIPYTTTLTLVKQLARFKIQIIAPGKTHTAAIDERGRLMTFGCNKYGQLGVKDFKRHQGVQILLGPFGGKVVSKVSCGDGFTIAATEDNQIFAWGNAGNGRLGMPADRGFGSEVCPALPRPIFGSLHHVPDLSCRGWHTILIMEKVLNSKTIRSNSSGL 728
BLAST of NEK8-1 vs. Ensembl Cavefish
Match: nek8 (NIMA related kinase 8 [Source:NCBI gene;Acc:103024333]) HSP 1 Score: 330.872 bits (847), Expect = 6.546e-102 Identity = 216/646 (33.44%), Postives = 346/646 (53.56%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINE---IQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLN-SEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTW---SNIQGDKKLSGQLGHGAIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGI-NSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIV--AVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLG 2037
Y +++V+G+GA+G L R VD +LV+ KEI +++ +E QNE +L +L+HPNI+ YY +F+ + I MEYA G+L++Y+ ++ L E +L +F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ +L +P+KRP +++ P+ C + ++ T+ N R S + + SL S V+ WG G L++ + LQV+ G + + +T L TW S G+ L G + + I + ++ GV IK VSCG+ T C++ G + GS G LG N N+ E + + + Q+SCG H A+ E +F+WG G+ GRLGLG +D P VD+P + + + V CG D + I+ ++L CG+N++NKLG++ ++ + + C V F+ V A L K+ + G +H+A + + + FG N +GQ+G ++ + V + + +CGD FT+ T E++ WG+ GRLG
Sbjct: 4 YEKIKVVGRGAFGIVHLCRRRVDGALVILKEIPVEQMTRDERLAAQNECQVLKLLNHPNIIEYYENFLEDKALMIAMEYAPGGTLADYIQKRCNSLLDEDTILHFFVQILLALYHVHNKLILHRDLKTQNILLDKHQMIVKIGDFGISKILVSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLILTMLNLDPSKRPQLNEIMALPI----CIRPLLNLY-TDIGNVKMRGLSSLSSTKMMHSLPLSSVYTWGSGISTPLRLPMLNTE--VLQVSLGRTQKMGVTKSGRLITWEAPSVGSGEPSLPGAVEQMQPQF-ISRFLEGQSGVTIKSVSCGDLFTTCMTDRGIIMTFGSGSNGCLGHGNFNDVTQPKIVEALLGYELV--------QVSCGASHVLAVTN-EREVFSWGRGDNGRLGLGTQDTHNAPQQVDVP-AEFEAQRVLCGVDCSMII-STQHQILACGSNRFNKLGLD----KIADTEEPPSCCQVEEVQTFQPVQSAPLNVEKIVYIDIGTAHSAAVTEKGQCFTFGSNQHGQLGCSSRRSSR---VPYPVPGLQGVTTAACGDAFTLAIGTEGEVYTWGKGARGRLG 623 HSP 2 Score: 56.225 bits (134), Expect = 1.383e-7 Identity = 32/90 (35.56%), Postives = 47/90 (52.22%), Query Frame = 1
Query: 1777 VKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFA--GKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGL 2040
V + +VSCG+ T + + FG S G +G G+ DV + V A G ++ ++SCG + T E+F WGR D+GRLGL
Sbjct: 370 VTIKSVSCGDLFTTCMTDRGIIMTFGSGSNGCLGHGN---FNDVTQPKIVEALLGYELVQVSCGASHVLAVTNEREVFSWGRGDNGRLGL 456
BLAST of NEK8-1 vs. Ensembl Cavefish
Match: nek8 (NIMA related kinase 8 [Source:NCBI gene;Acc:103024333]) HSP 1 Score: 326.25 bits (835), Expect = 6.996e-100 Identity = 218/669 (32.59%), Postives = 350/669 (52.32%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINE---IQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVW----------KGNCQ-SMIDQVHRTNQANSNSRP---CSGDRKERSIVSLN----------SEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTW---SNIQGDKKLSGQLGHGAIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGI-NSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIV--AVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLG 2037
Y +++V+G+GA+G L R VD +LV+ KEI +++ +E QNE +L +L+HPNI+ YY +F+ + I MEYA G+L++Y+ ++ L E +L +F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ +L +P+KRP +++ P+ GN + I++ + Q + R +G R + SL+ S V+ WG G L++ + LQV+ G + + +T L TW S G+ L G + + I + ++ GV IK VSCG+ T C++ G + GS G LG N N+ E + + + Q+SCG H A+ E +F+WG G+ GRLGLG +D P VD+P + + + V CG D + I+ ++L CG+N++NKLG++ ++ + + C V F+ V A L K+ + G +H+A + + + FG N +GQ+G ++ + V + + +CGD FT+ T E++ WG+ GRLG
Sbjct: 4 YEKIKVVGRGAFGIVHLCRRRVDGALVILKEIPVEQMTRDERLAAQNECQVLKLLNHPNIIEYYENFLEDKALMIAMEYAPGGTLADYIQKRCNSLLDEDTILHFFVQILLALYHVHNKLILHRDLKTQNILLDKHQMIVKIGDFGISKILVSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLILTMLNLDPSKRPQLNEIMALPICIRPLLNLYTDIGNVKMRRIEKQLSSVQTGPHGRAGGRVTGTRTRGGLSSLSSTKMMHSLPLSSVYTWGSGISTPLRLPMLNTE--VLQVSLGRTQKMGVTKSGRLITWEAPSVGSGEPSLPGAVEQMQPQF-ISRFLEGQSGVTIKSVSCGDLFTTCMTDRGIIMTFGSGSNGCLGHGNFNDVTQPKIVEALLGYE--------LVQVSCGASHVLAVTN-EREVFSWGRGDNGRLGLGTQDTHNAPQQVDVP-AEFEAQRVLCGVDCSMII-STQHQILACGSNRFNKLGLD----KIADTEEPPSCCQVEEVQTFQPVQSAPLNVEKIVYIDIGTAHSAAVTEKGQCFTFGSNQHGQLGCSSRRSSR---VPYPVPGLQGVTTAACGDAFTLAIGTEGEVYTWGKGARGRLG 651 HSP 2 Score: 55.8398 bits (133), Expect = 1.542e-7 Identity = 32/90 (35.56%), Postives = 47/90 (52.22%), Query Frame = 1
Query: 1777 VKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFA--GKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGL 2040
V + +VSCG+ T + + FG S G +G G+ DV + V A G ++ ++SCG + T E+F WGR D+GRLGL
Sbjct: 398 VTIKSVSCGDLFTTCMTDRGIIMTFGSGSNGCLGHGN---FNDVTQPKIVEALLGYELVQVSCGASHVLAVTNEREVFSWGRGDNGRLGL 484
BLAST of NEK8-1 vs. Ensembl Cavefish
Match: nek12 (serine/threonine-protein kinase Nek4-like [Source:NCBI gene;Acc:103035324]) HSP 1 Score: 182.185 bits (461), Expect = 2.217e-49 Identity = 112/286 (39.16%), Postives = 166/286 (58.04%), Query Frame = 1
Query: 133 SYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDID---KNKINE-IQNEIDILSILDHPNIVAYYNHFVYIDT----VFIEMEYANAGSLSNYLRQLKED--LSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMID-QVHRTNQANSNSRP 954
+Y Q+ LG G G +L R+ N L K I +D K K E + E +IL L HP+IV + + + DT ++I M+Y + G+L + ++ K D SE + +F Q++ A+ YIH I+HRDIKT NI LT ++K+GDFGISK+++ + A+T VGTP Y+SPE+ Q +PYS+KSDIWA GC+LYE+ + K F N L+L KI+ GE+ P+ + DL L++++L P RPSA +L P + + I+ Q + N SRP
Sbjct: 3 TYEQILTLGSGGAGVVLLMRHKETNKLYAVKRIQVDNSRKTKTKEAVLQEAEILRNLKHPHIVTW--SYTFYDTANELIYIAMDYCDGGTLDDQIKGRKPDEYFSEHVTMKWFVQVLMAVSYIHSKKILHRDIKTSNILLTKRGMIKLGDFGISKIMSHTLDMASTCVGTPCYLSPELCQDIPYSTKSDIWALGCLLYELCTLKPAFWARNLLNLFYKILKGEYSPAPDQYSKDLHSLIEKMLCLVPENRPSASSILGMPYVQEHLSQFIEHQETELTKVNVESRP 286
BLAST of NEK8-1 vs. Ensembl Cavefish
Match: nek12 (serine/threonine-protein kinase Nek4-like [Source:NCBI gene;Acc:103035324]) HSP 1 Score: 182.185 bits (461), Expect = 5.249e-49 Identity = 112/286 (39.16%), Postives = 166/286 (58.04%), Query Frame = 1
Query: 133 SYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDID---KNKINE-IQNEIDILSILDHPNIVAYYNHFVYIDT----VFIEMEYANAGSLSNYLRQLKED--LSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMID-QVHRTNQANSNSRP 954
+Y Q+ LG G G +L R+ N L K I +D K K E + E +IL L HP+IV + + + DT ++I M+Y + G+L + ++ K D SE + +F Q++ A+ YIH I+HRDIKT NI LT ++K+GDFGISK+++ + A+T VGTP Y+SPE+ Q +PYS+KSDIWA GC+LYE+ + K F N L+L KI+ GE+ P+ + DL L++++L P RPSA +L P + + I+ Q + N SRP
Sbjct: 3 TYEQILTLGSGGAGVVLLMRHKETNKLYAVKRIQVDNSRKTKTKEAVLQEAEILRNLKHPHIVTW--SYTFYDTANELIYIAMDYCDGGTLDDQIKGRKPDEYFSEHVTMKWFVQVLMAVSYIHSKKILHRDIKTSNILLTKRGMIKLGDFGISKIMSHTLDMASTCVGTPCYLSPELCQDIPYSTKSDIWALGCLLYELCTLKPAFWARNLLNLFYKILKGEYSPAPDQYSKDLHSLIEKMLCLVPENRPSASSILGMPYVQEHLSQFIEHQETELTKVNVESRP 286
BLAST of NEK8-1 vs. Ensembl Sea Lamprey
Match: NEK9 (NIMA related kinase 9 [Source:HGNC Symbol;Acc:HGNC:18591]) HSP 1 Score: 513.072 bits (1320), Expect = 5.800e-171 Identity = 302/768 (39.32%), Postives = 451/768 (58.72%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDI----DKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSL-SNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQT-MPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKER--SIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGH-GAIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIIN---QISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVST-TNNEIFGWGRADSGRLGL---EITVDQRCVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTI-SANSTAIMDKLSLKNVNSF-----------ETEYDKDGS------------------GDIQTWLRKELQE 2298
Y+ VRVLG+GA+G+A LYR DNSLVVWKEID+ DK + + QNEIDILS+L+H NI++YYNHF+ + + IE+EY N G+L ++Q E ++WYF+QL+ A++ IH I+HRDIKTLNIFLT S L+K+GDFGISK+L+ + A + VGTP YMSPE+I+G Y++KSDIWA GCVLYE+++ + F+ +N L L +II+G+ Q + + + ++ +++Q+ L + +RP+AED+L+ K + M V R N+ +R D E + + +S+V+ WGGGK ++QF+ SAL+V A +HF +T EK+LYTW+N+QG K+ GQLGH Y+ PK V L G+ +++V+CG+E T+CL+ GE+F GS+YYG +G N + V +P+ IN QISCG H A+ ++ WGCGEYGRLGL + + P V + ++ K+ V CG D TF + ++L CGNN++NKLG+N GL + + ++ ++V + +V L K+ ++S G +H+AV+D+ +L FG N +GQ+G G K H + GK I +SCGD FTI +T +N I+ WG D+GRLG+ E + L PR +FG+L +D+ GW TI+I E++L + TI S + + +L N +F E+ YD+D + WLR+EL+E
Sbjct: 36 YIPVRVLGRGAFGEAALYRRNEDNSLVVWKEIDLTRLSDKER-RDSQNEIDILSLLEHANIISYYNHFLDGNVLLIELEYCNGGTLYEKIIKQHGAFFPEKDIVWYFYQLVSAVEQIHNFKILHRDIKTLNIFLTKSDLIKVGDFGISKILDSRSQMAESYVGTPYYMSPELIRGDKYNTKSDIWAMGCVLYELLTLTRTFDATNPLKLCYEIIHGKKQVEIDDQYSKEIKEVVQKCLQQDAEQRPTAEDLLELSPIKTRRREMEKNVWRLNEP---ARRIRSDNAEAVPVVTARSSDVYYWGGGKRTPQMLDQFRGGYSALRVCASNTHFAAVTVEKELYTWANVQGGTKMVGQLGHKDKASYRQPKRVDKLLGLAVRQVACGDEFTVCLTEQGEVFSFGSDYYGCMGCNGE--------LGVEVLEPMPVQFFNINPVTQISCGDCHVMALTR-SGAVYAWGCGEYGRLGLETDATEFYPKRVPLS-NRWKITEVCCGTDGTFFL-TAQGKVLACGNNEFNKLGLNQCTSGLR-NRETSVFHEVIMKLMPTLVKQLSIYKIRSISGGKTHSAVIDVCGRLLTFGHNKHGQLGTGDFKKHLGIVLHKGPLVGKLITMVSCGDGFTIAATEADNHIYAWGNTDNGRLGVMPSEKPGNSPLAL-PRPIFGSLHVVSDLSCRGWHTILIAEKVLTSKTIRSGGNFSARSSFTLSNNKTFFPRSSLGETEDESGYDEDSAKSQFDPPTYEESESSTAGSSCPEWLRRELEE 786
BLAST of NEK8-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001750.1 (pep scaffold:Pmarinus_7.0:GL481695:1137:12922:1 gene:ENSPMAG00000001576.1 transcript:ENSPMAT00000001750.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 276.559 bits (706), Expect = 6.703e-83 Identity = 198/611 (32.41%), Postives = 295/611 (48.28%), Query Frame = 1
Query: 370 AGSLSNYLRQLKEDL-SESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFL-TTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMI-------------------DQVHRTNQANSNSRPCSGDRKERSIVSLN-------SEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTW---SNIQGDKKLSGQLGHGAIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINS-NETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHED--NIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNN-QYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAV----LRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQRCVLHPRAL 2085
G+L+ Y+++ L E +L +F Q+ AL ++H HI+HRD+KT NI L T++KIGDFGISK+L+ A T VGTP Y+SPE+ +G PY+ KSDIWA GC+LYE+ S K+ FE N +L +KI++G F + + + +L L+ LL +P+KRP +VL HPV C S + + + + A+ + P SGD S V++WG G + +K+ LQVA G + +T L W GD L G +G + + ++ GV I+ VSCG+ T CL+ G + GS G LG S ++ E + + I Q+SCG H A+ E +F WG G+ GRLGLG+++ +P V P C +CG D + + S +LL CG+N +YNKLG++ +S C E + V L +V V+ G +H+A + Y FG N +GQ+G G +T + G K+ +++CGD FT+ ++F WG+A GRLG T + CV P L
Sbjct: 1 GGTLAEYIQKRGGSLLDEDTILHFFVQIALALHHVHSNHILHRDLKTHNILLDKRQTVVKIGDFGISKILSSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCILYELASLKRAFEAENLPALVLKIMSGNFAPVSDRYSGELRQLVVSLLNLDPSKRPQLSEVLSHPV----CMSAMIRLYMDIGRVPARFVSGEPELISPSVSASRPTAPVSGDGAPAGSAGRAGAAPPPLSSVYMWGSGTLAPVKLA--MPNTEVLQVAVGRTQRAGVTRSGRLIVWEASPGTVGDAALPGSVGS-VQPQLVSRFLEGQSGVTIQHVSCGDLFTACLTDRGILMTYGSGSSGCLGHGSYSDVAQPKIVEALLGYE--------ITQMSCGMSHVLAVSN-EQEVFAWGRGDNGRLGLGNQELHLCPQPVAVPPPYEPCT---AHCGIDCSMVTS--SGKLLACGSNRRYNKLGLD----KISHDGDDAACPAEQTEESLVLSPVQSSPLDGARVVHVALGTAHSAAVTDTGTCYTFGSNQHGQLG-GGARTSSRAPQPVDGLGGVKVVQVTCGDAFTVAVAEGGQVFTWGKAARGRLGR--TDGEACVPGPVQL 583
BLAST of NEK8-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007414.1 (pep scaffold:Pmarinus_7.0:GL476981:230882:244405:1 gene:ENSPMAG00000006689.1 transcript:ENSPMAT00000007414.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 182.57 bits (462), Expect = 8.974e-52 Identity = 102/263 (38.78%), Postives = 155/263 (58.94%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDK---NKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDL-SESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMI 909
Y V +G+G++GKA+L ++ D L V KEI+I + + +E + E+ +L+ + HPNIV+Y F ++I M+Y G L + K L SE +L + QL AL ++H I+HRDIK+ NIFLT + ++++GDFGI++VLN E A T +GTP Y+SPE+ + PY+ KSD+WA GCVLYE+ + + FE N +L +KII G F + +S+L L LL +P RP +L P+ Q ++
Sbjct: 4 YTMVSQIGEGSFGKAMLVKSKADGHLYVIKEINISRMSPKERDEARKEVCVLANMRHPNIVSYKESFEERGNLYIVMDYCEGGDLFKRINAQKGVLFSEDQILDWLVQLCLALKHVHDRKILHRDIKSQNIFLTKAGIVQLGDFGIARVLNSTTELARTCIGTPYYLSPEICENKPYNHKSDMWALGCVLYEMCTLRHAFEAGNMKNLVLKIIRGSFPPVSAHYSSELRSTLSGLLRRDPRARPPVAVLLSRPLLMARAQRLM 266
BLAST of NEK8-1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000555.1 (pep scaffold:Pmarinus_7.0:GL477093:35206:42652:1 gene:ENSPMAG00000000500.1 transcript:ENSPMAT00000000555.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 134.806 bits (338), Expect = 1.093e-36 Identity = 75/165 (45.45%), Postives = 104/165 (63.03%), Query Frame = 1
Query: 403 KEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSN-----HLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHP 879
K+ ++++ V+ +F Q+ A+ YIH + I+HRDIKT N+FLT + +K+GDFGISK + + A T VGTP Y+SPE+ Q LPYSSKSD+WA GC+LYE+ + + FE SN SL KI+ GE + + D+ L+ LL P RPSA VL P
Sbjct: 2 KQIITKTNVMQWFVQVCMAVQYIHSVKILHRDIKTSNVFLTKAGTVKVGDFGISKAMEGTLDMAVTCVGTPYYLSPEMCQDLPYSSKSDVWALGCLLYELCALRPAFEASNIIRLTRRSLFYKIMRGEPDPLDESYSRDVRALVALLLSRSPEDRPSASAVLASP 166
BLAST of NEK8-1 vs. Ensembl Sea Lamprey
Match: nek2 (NIMA-related kinase 2 [Source:ZFIN;Acc:ZDB-GENE-040426-752]) HSP 1 Score: 139.813 bits (351), Expect = 9.908e-36 Identity = 78/205 (38.05%), Postives = 116/205 (56.59%), Query Frame = 1
Query: 211 LVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYID--TVFIEMEYANAGSLSNYLRQLKED---LSESCVLWYFFQLIYALDYIHKLH-----IIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMP 783
++VWK +D E Q +E+++L L HPNIV Y++ + T++I MEY G L++ + + + D L E VL QL+ AL H+ ++HRD+K N+FL +K+GDFG++++L D FA T VGTP YMSPE + Y+ KSDIW+ GC+LYE+ + F N LA KI G+F+ +P
Sbjct: 2 VLVWKVLDYGTMSEPEKQMLVSEVNLLRELKHPNIVRYFDRIIDRSNTTLYIVMEYCECGDLASLISRCRRDRRFLEEEFVLRVLAQLVLALRECHRRTAGGRTVLHRDLKPANVFLDARHNVKLGDFGLARILQHDTSFAKTFVGTPYYMSPEQFSRMSYNEKSDIWSLGCLLYELCALVPPFTAMNQKQLAEKIKEGKFRRIP 206
BLAST of NEK8-1 vs. Ensembl Yeast
Match: GIN4 (Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication [Source:SGD;Acc:S000002915]) HSP 1 Score: 122.479 bits (306), Expect = 7.766e-29 Identity = 71/215 (33.02%), Postives = 127/215 (59.07%), Query Frame = 1
Query: 259 IQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSS-KSDIWASGCVLYEIISRKKLF--EYSNHLSLAMKIINGEFQTMPNCN--NSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWK 888
I+ EI I+ +L+HPN++ Y+ + +++ +EYA G L N L + + L E + +F Q+I + Y H L I+HRD+K N+ L +KI DFG++ + E + T G+P Y +PE++ G+PY SD+W+ G +L+ +++ + F E N +L +K+ GEF+ MP+ + + + DL++++L +P +R D+LKHP+ +
Sbjct: 78 IEREIIIMKLLNHPNVLRLYDVWETNTDLYLVLEYAEKGELFNLLVE-RGPLPEHEAIRFFRQIIIGVSYCHALGIVHRDLKPENLLLDHKYNIKIADFGMAALETEGKLLETSCGSPHYAAPEIVSGIPYQGFASDVWSCGVILFALLTGRLPFDEEDGNIRTLLLKVQKGEFE-MPSDDEISREAQDLIRKILTVDPERRIKTRDILKHPLLQ 290
BLAST of NEK8-1 vs. Ensembl Yeast
Match: TPK3 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication [Source:SGD;Acc:S000001649]) HSP 1 Score: 113.62 bits (283), Expect = 2.297e-27 Identity = 69/235 (29.36%), Postives = 119/235 (50.64%), Query Frame = 1
Query: 91 VLSNRDFSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEID----IDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMP 783
+L RD S ++ + +R LG G++G+ L R+ + K + + ++ +E +LSI+ HP I+ + F VF+ M+Y G L + LR+ + +Y ++ AL+Y+H II+RD+K NI L + +KI DFG +K + + + T GTP Y++PEV+ PY+ D W+ G ++YE+++ F SN + I+N E + P
Sbjct: 73 MLQYRDTSGKYSLSDFQILRTLGTGSFGRVHLIRSNHNGRFYALKTLKKHTIVKLKQVEHTNDERRMLSIVSHPFIIRMWGTFQDSQQVFMVMDYIEGGELFSLLRK-SQRFPNPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILLDKNGHIKITDFGFAKYVPDVTY--TLCGTPDYIAPEVVSTKPYNKSVDWWSFGVLIYEMLAGYTPFYNSNTMKTYENILNAELKFPP 304
BLAST of NEK8-1 vs. Ensembl Yeast
Match: SNF1 (AMP-activated S/T protein kinase; forms a complex with Snf4p and members of the Sip1p/Sip2p/Gal83p family; required for transcription of glucose-repressed genes, thermotolerance, sporulation, and peroxisome biogenesis; regulates nucleocytoplasmic shuttling of Hxk2p; regulates filamentous growth and acts as a non-canonical GEF, activating Arf3p during invasive growth; SUMOylation by Mms21p inhibits its function and targets Snf1p for destruction via the Slx5-Slx8 Ub ligase [Source:SGD;Acc:S000002885]) HSP 1 Score: 115.931 bits (289), Expect = 2.945e-27 Identity = 73/221 (33.03%), Postives = 121/221 (54.75%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEID---IDKNKIN-EIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKS-DIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMP 783
Y V+ LG+G++GK L + V K I+ + K+ + I+ EI L +L HP+I+ Y+ D + + +EYA L +Y+ Q ++ +SE +F Q+I A++Y H+ I+HRD+K N+ L +KI DFG+S ++ + F T G+P Y +PEVI G Y+ D+W+ G +LY ++ R+ F+ + L I NG + T+P
Sbjct: 55 YQIVKTLGEGSFGKVKLAYHTTTGQKVALKIINKKVLAKSDMQGRIEREISYLRLLRHPHIIKLYDVIKSKDEIIMVIEYA-GNELFDYIVQ-RDKMSEQEARRFFQQIISAVEYCHRHKIVHRDLKPENLLLDEHLNVKIADFGLSNIMTDGNFLKTSCGSPNYAAPEVISGKLYAGPEVDVWSCGVILYVMLCRRLPFDDESIPVLFKNISNGVY-TLP 272
BLAST of NEK8-1 vs. Ensembl Yeast
Match: TPK1 (cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication [Source:SGD;Acc:S000003700]) HSP 1 Score: 113.235 bits (282), Expect = 2.990e-27 Identity = 69/220 (31.36%), Postives = 116/220 (52.73%), Query Frame = 1
Query: 145 VRVLGKGAYGKAVLYRNAVDNSL----VVWKEIDIDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCN 792
+R LG G++G+ L R+ + V+ KEI + ++ +E +LSI+ HP I+ + F +F+ M+Y G L + LR+ + +Y ++ AL+Y+H II+RD+K NI L + +KI DFG +K + + + T GTP Y++PEV+ PY+ D W+ G ++YE+++ F SN + KI+N E + P N
Sbjct: 90 LRTLGTGSFGRVHLIRSRHNGRYYAMKVLKKEIVVRLKQVEHTNDERLMLSIVTHPFIIRMWGTFQDAQQIFMIMDYIEGGELFSLLRK-SQRFPNPVAKFYAAEVCLALEYLHSKDIIYRDLKPENILLDKNGHIKITDFGFAKYVPDVTY--TLCGTPDYIAPEVVSTKPYNKSIDWWSFGILIYEMLAGYTPFYDSNTMKTYEKILNAELRFPPFFN 306
BLAST of NEK8-1 vs. Ensembl Yeast
Match: KCC4 (Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication [Source:SGD;Acc:S000000529]) HSP 1 Score: 115.161 bits (287), Expect = 1.071e-26 Identity = 72/215 (33.49%), Postives = 123/215 (57.21%), Query Frame = 1
Query: 259 IQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSS-KSDIWASGCVLYEIISRKKLF--EYSNHLSLAMKIINGEFQTMPNCN--NSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWK 888
I+ EI I+ +L HPN+++ Y+ + + +++ +EYA G L N L L E + F Q+I + Y H L I+HRD+K N+ L + +KI DFG++ + + + T G+P Y +PE++ GLPY SD+W+ G +L+ +++ + F E N L +K+ G+F+ MPN + D DL+ ++L+ +P +R D+L HP+ K
Sbjct: 74 IEREIVIMKLLSHPNVLSLYDVWETNNNLYLILEYAEKGELFNLLVD-HGPLPEREAINCFRQIIIGISYCHALGIVHRDLKPENLLLDSFYNIKIADFGMAALQTDADLLETSCGSPHYAAPEIVSGLPYEGFASDVWSCGVILFALLTGRLPFDEENGNVRDLLLKVQKGQFE-MPNDTEISRDAQDLIGKILVVDPRQRIKIRDILSHPLLK 286
BLAST of NEK8-1 vs. Ensembl Nematostella
Match: EDO35699 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SKU3]) HSP 1 Score: 473.781 bits (1218), Expect = 1.340e-157 Identity = 248/624 (39.74%), Postives = 392/624 (62.82%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDI----DKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIV-SLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCED-LLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVST 1986
Y+ VR LG+GA+G+AVLYR DN LVVWKE+++ D+ + + ++ EIDILS+L+H NIV Y+NHF+ ++FIEMEY N G+L + + E E V+WYFFQ+ A+ +IH+ I+HRDIKT+NIFLT S L+K+GDFGISK+L+ + A++ VGTP YMSPE++QG Y+ KSD+WA GCVLYE+++ K++F+ SN L L I+ G ++ + ++ L+ +LL P RPS +++L+ P+ + M +V + N + +R + + ++ S EV+ WG GK K++ F ++A QVAAG SHF ++T EK+++TW+N G K +SGQLGHG + Y+ PK V +L G+ IK+V+CG+E T C++ +G ++ GS+Y+G +G ++ E + K+T + DK + ISCG H + C + +++WGCGE+GRLGLG ED+ P + +P CK+K +YCG ++TF++ + R+L CGNN N+LG++ L ++++ VP K+V L ++ V+ G +H+A +D+Y +L G N YGQ+G+ + H + K++ + +CG+ FT+V+T
Sbjct: 17 YIPVRTLGRGAFGEAVLYRKIEDNMLVVWKEVNLSRASDRERADALK-EIDILSLLNHTNIVTYFNHFIDGTSLFIEMEYCNGGTLHDKICVQNELFPEEVVIWYFFQICAAVGHIHENGIVHRDIKTMNIFLTKSGLVKLGDFGISKILDSEGMADSIVGTPYYMSPEIVQGKKYNQKSDMWAVGCVLYEVLTLKRVFDASNPLRLVSDIVKGHYEEIDERYTEEMNSLVNKLLSQNPDDRPSVQELLEMPILATLGKEMEKKVWQLNSSTRRARLVTHSSETVPVITSKTCEVYFWGAGKQTPQKVDTFVLGNTATQVAAGHSHFAIVTVEKEMFTWANAHGGKGMSGQLGHGDVACYRNPKRVDALFGIPIKKVACGDEFTACITDDGALYTFGSDYFGCIGCSNELDEEVLLPHKVTFFN--DKP---VEDISCGDCHVVVM--CANKAIYSWGCGEHGRLGLGSEDDFAFPQKIPLPT-SCKVKRIYCGPESTFLL-TVTGRVLACGNNDENQLGLDT----LHSLRKRSIKGHVPSANSPKLVQSLNRYTIAIVAPGKTHSAFVDVYGRLVTVGSNKYGQLGVNDYQHHPSPCQVKGELVSKQVVEAACGEEFTVVAT 626 HSP 2 Score: 61.2326 bits (147), Expect = 1.377e-9 Identity = 39/108 (36.11%), Postives = 54/108 (50.00%), Query Frame = 1
Query: 1723 CYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKV--FAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGL 2040
CY P K V L + + V+CG+ TA + LY FG + +G IG + + ++V KV F K +E ISCGD +V N I+ WG + GRLGL
Sbjct: 374 CYRNP-----KRVDALFGIPIKKVACGDEFTACITDDGALYTFGSDYFGCIGCSN-ELDEEVLLPHKVTFFNDKPVEDISCGDCHVVVMCANKAIYSWGCGEHGRLGL 475 HSP 3 Score: 52.7582 bits (125), Expect = 4.672e-7 Identity = 55/215 (25.58%), Postives = 90/215 (41.86%), Query Frame = 1
Query: 1420 QISCGHHHAAAIGGCEDLLFTW-----GCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGK--KIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLE 2043
Q++ GH H A + E +FTW G G G+LG G P VD L +K V CG + T + L G++ + +G S++ + +P + F + V +SCG+ H V+ +Y +G +G++GLG + D K+ K+++I CG T + T + G D +LGL+
Sbjct: 334 QVAAGHSHFAIVT-VEKEMFTWANAHGGKGMSGQLGHGDVACYRNPKRVD-ALFGIPIKKVACGDEFTACITD-DGALYTFGSDYFGCIGC---------SNELDEEVLLPHKVTF-----FNDKPVEDISCGDCHVVVMCANKAIYSWGCGEHGRLGLG---SEDDFAFPQKIPLPTSCKVKRIYCGPESTFLLTVTGRVLACGNNDENQLGLD 528
BLAST of NEK8-1 vs. Ensembl Nematostella
Match: EDO46185 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQY7]) HSP 1 Score: 344.739 bits (883), Expect = 1.771e-107 Identity = 229/690 (33.19%), Postives = 356/690 (51.59%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDL-SESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTST-LLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTN---------------QANSNSRPCSGDRKERSIVSLNSE-----VFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYK---IPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKV--------STVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAG-KKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITVDQR---CVLHPRAL 2085
Y +VR++G+GAYG L R VDN LV+ K+I +++ E Q NE+ +LS+ HPNI+ YY+ FV + I MEYA G++ +YL+Q L E +L F Q++ AL ++HK I+HRD+KT NI L ++KIGDFGISK+L+ ANT +G+P Y+SPE+ +G PY+ KSD+W+ GCVLYE+ + K+ FE SN +L +KI+ G F +P + +L L+ +L+ +PTKRP ++++ PV + V R + +S S G + + ++ + V+ WGGG + Q +QV+ G + +TA + W K SG + G K +P+ ++ G+ I +VSCG+ T CL+ G + GS YG LG T ++Q + + D + ISCG H A+ + +F WG G+ GRLG G + + P + +P + + V+CG D +F + ++LL CG+N+ NKL ++ D P E VAVLR V V S+V+ G SHT VL KLY FG NS+GQ+G + V++ ++ + ++CGD F++ ++++E+F WG+ GRLG + D R C+ P A+
Sbjct: 4 YEKVRIVGRGAYGTVYLCRRLVDNFLVIIKQIPVEEMTKEERQSALNEVKVLSMFQHPNIIRYYDSFVQEKALMIVMEYAQGGTIYDYLQQRGGKLMDEDEILRLFVQILLALRHVHKGQILHRDLKTQNILLNKKRKVVKIGDFGISKILSSKSKANTVIGSPCYISPELCEGKPYNQKSDVWSLGCVLYELTTLKRAFEASNLPALVLKIMRGYFSPIPERYSEELRKLILDMLVLDPTKRPGIKEIMAQPVIVNALFDLPTDVGRVPCTRFPRTGAGIAGMYEESSASTILEGLPENLGVFAMKPQEPQHMVYHWGGGITYPNPLPLPQHDSGVVQVSCGRTLMTGVTANGRMILWEG----SKSSGLMSSGGKNEKTVWVPRFLEGQSGITITKVSCGDLFTACLTDRGILMTFGSGTYGCLG--HGTTNYVSQAKIVE-----DLLGFEVEDISCGSSHIMAVTADHE-VFAWGRGDNGRLGTGSQQEYWSPKAISLP-EGMRARSVHCGVDCSFAI-TLENKLLACGSNRCNKLALDNE--------------DGPVEE----VAVLRQVSVPDFNSVGTSSVAMGTSHTLVLLQNGKLYSFGSNSFGQLGY----VREGVSRFPQLVESLNDVSYVACGDTFSVAVSSDSEVFVWGKGARGRLGNKADEDVREPVCLSLPAAV 657
BLAST of NEK8-1 vs. Ensembl Nematostella
Match: EDO49822 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG20]) HSP 1 Score: 182.57 bits (462), Expect = 5.280e-52 Identity = 104/251 (41.43%), Postives = 156/251 (62.15%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLR-QLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLK 873
YV+V+ +G+G++GKA+L + D V KEI+I + K E + E+ +LS L HPNIV+Y F ++I M+Y + G L + Q SE V+ +F Q+ L ++H I+HRDIK+ NIFLT ++K+GDFGI++VL+ E A T +GTP Y+SPE+++ PY++KSDIW+ GCVLYE+++ K FE N +L +KII G + +P ++D+ L+ +LL P RPS VLK
Sbjct: 4 YVRVKKIGEGSFGKALLVKKRNDGKHYVIKEINICRMKPREREESRKEVKVLSQLKHPNIVSYQESFEESGNLYIVMDYCDGGDLYKKINGQRGIAFSEDQVMDWFVQICLGLKHVHDRKILHRDIKSQNIFLTRHGIIKMGDFGIARVLHSTVELARTCIGTPYYLSPEIVENRPYNNKSDIWSLGCVLYEMLTLKHAFEAGNMKNLVLKIIRGSYPPIPLRYSADIRMLVAQLLKRNPHDRPSVNTVLK 254
BLAST of NEK8-1 vs. Ensembl Nematostella
Match: EDO49690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RG02]) HSP 1 Score: 180.259 bits (456), Expect = 5.109e-51 Identity = 102/263 (38.78%), Postives = 159/263 (60.46%), Query Frame = 1
Query: 121 LNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQN---EIDILSILDHPNIVAYYNHFVYIDT--VFIEMEYANAGSLSNYLRQLKE-DLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVL-NEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWK 888
+++ Y + +GKG+YG+ L ++ D V K++D+ K + E + E +LS L HPNIV+Y F DT ++I M + G L + L+ K L E+ ++ +F Q+ AL Y+H+ HI+HRD+KT NIFLT S ++K+GD GI++VL + + A T +GTP YMSPE+ PY+ KSD+WA GC LYE+ + + F + SL KI+ G+ +P ++DL +++ +L +P KRPSA +L+HP K
Sbjct: 1 MSLDRYTIGKCIGKGSYGEVFLSKHKKDKKQYVLKKVDLQKASVRERKAAELEAKLLSQLRHPNIVSYRESFQ-DDTGFLYIAMNFCEGGDLYSRLKAQKGIPLDENQIVEWFVQIAMALQYMHEKHILHRDLKTQNIFLTKSKIIKVGDLGIARVLESSSDMATTLIGTPYYMSPELFSNKPYNHKSDVWALGCCLYEMCTLRHAFNAKDMSSLVYKILKGKTPPLPKQYSTDLCSIIKSMLDQDPDKRPSASRLLRHPYIK 262
BLAST of NEK8-1 vs. Ensembl Nematostella
Match: EDO49138 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RH03]) HSP 1 Score: 175.637 bits (444), Expect = 1.038e-49 Identity = 101/252 (40.08%), Postives = 146/252 (57.94%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHP 879
Y ++ ++GKG +G A L + L KE++ + NE+ ILS L H NIV Y + F +I MEYA G L ++Q E S +L + QL+ AL +IH +I+HRD+KT NIFLT ++KIGDFGI+++L+ + A T VGTP Y+SPE+ Q PY++KSDIWA GCVLYE+ +R F N +L +KI++G + +P L DL+ +L P RPSA+ ++ P
Sbjct: 4 YQKIDIIGKGTFGSAWLVESRQSKRLYALKELNATAMPSEDRHLALNEVKILSTLKHRNIVRYRDAFEETGRFYIVMEYAEGGDLHTKIKQAVEPFSSERILNWLAQLLLALKHIHGQNILHRDLKTQNIFLTKDDVVKIGDFGIARILDSTCDHARTTVGTPYYLSPEICQRQPYNNKSDIWALGCVLYELTTRTHPFTADNLTNLVVKILHGNYPPIPRFYGPLLEDLVAVMLKINPADRPSAKQLIYVP 255
BLAST of NEK8-1 vs. Ensembl Medaka
Match: NEK9 (NIMA related kinase 9 [Source:NCBI gene;Acc:101166349]) HSP 1 Score: 535.798 bits (1379), Expect = 5.484e-177 Identity = 292/702 (41.60%), Postives = 439/702 (62.54%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDID---KNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKE-DLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLN-EDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEF--QTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSI--VSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHG-AIVYKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSG-SSQKNLCYD-VPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGLEITV---DQRCVLHPRALFGTLQKCADIQSFGWQTIMITERILETSTISANSTAI 2196
Y+ +R+LG+GA+G+A LYR DNSLVVWKE+D++ + + ++ NEI+ILS+L+H NI+AY+NHF+ +++ IE+EY N G+L + + Q K SE V+WY +Q+ A+ YIHK I+HRDIKTLNIFLT + L+K+GD+G++K L+ E A T VGTP YMSPE+ QG Y+ KSDIWA GCVLYE+++ K+ F+ +N L+L +KI+ G + + + + +S LL L+ L +P KRP+AE +L P Q + +QV N +S RP E SI V+ + EV+ WGGGK K++ F+ A V AG +HF V+T EK+LYTW+++QG K+ GQLGHG Y+ PK V+ L+G I++V+CG + T C++ +M+ GS+Y G +G+ E ++ +Q + + + + PV + +SCG +H + +D +++WGCGEYGRLGL ED P V +P+ + V CG D+TF + + S ++L CGNN++NKLG+N QG+SG + Y +P+ T +V L K+ ++S G +HTA +D +L FG N +GQ+G+ K H V F GK + K++CGD FTI +T +N+IF WG A +GRLG+ + C PR +FG+L D+ GW TI+I E++L + TI +NS+ +
Sbjct: 41 YIPIRILGRGAFGEATLYRRTEDNSLVVWKEVDLNSLSEKERRDVMNEINILSVLEHNNIIAYFNHFMDKNSLLIELEYCNGGNLYDKIVQQKGVPFSEKVVVWYLYQIASAVAYIHKAGILHRDIKTLNIFLTKTDLIKLGDYGLAKKLDSEFSMAETCVGTPYYMSPELCQGAKYNFKSDIWAMGCVLYEVLTLKRTFDATNPLNLCVKIVQGNWTMEGVSDIYSSGLLKLVYECLHQDPAKRPTAEHILDQPSISCCRQELEEQVVLLN--SSMKRPKLNPVSESSIAVVTRSREVYYWGGGKFTPQKLDSFKAGSGAQHVCAGENHFAVVTVEKELYTWADVQGGAKMVGQLGHGDQASYRQPKRVEKLQGKAIRQVACGADFTACVTDEDQMYMFGSDYDGCIGV---EGQLGSQVLEPVLLEFFEGRPVQL--VSCGDNHVVVLTRDKD-IYSWGCGEYGRLGLESEDEFSSPMQVKVPI-GASISSVSCGSDSTFFLTE-SGKVLACGNNEFNKLGLN---QGISGIKNHPGEGYQGIPYTTAMTLVKQLSRFKIQSISPGKTHTAAIDERGRLITFGCNKFGQLGVKDFKKHAGVQVLVGPFGGKTVTKVACGDGFTIAATEDNQIFAWGNAGNGRLGMPPDKGFGSEVCPALPRPIFGSLHHVPDLSCRGWHTIIIMEKVLNSKTIRSNSSGL 729
BLAST of NEK8-1 vs. Ensembl Medaka
Match: nek8 (NIMA related kinase 8 [Source:NCBI gene;Acc:101161178]) HSP 1 Score: 304.294 bits (778), Expect = 1.361e-91 Identity = 216/670 (32.24%), Postives = 339/670 (50.60%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINE---IQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWK----------GNCQ-SMIDQVHRTNQANSNSRPCSGDRKERS-------------IVSLN-SEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYK-----IPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGI-NSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLG 2037
Y +++V+G+GA+G L R D + V+ KEI +++ +E QNE +L +L+HPNI+ YY +F+ + I MEYA G+L+ Y+ ++ L E +L +F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ +L +P+KRP +++ P+ GN + I++ Q RP G R S + SL S V+ WG G ++ LQV+ G + + +T L TW + +G+LG +V I + ++ GV IK VSCG+ T C++ G + GS G LG N N+ E + + + Q++CG H A+ E +F WG G+ GRLGLG +D P V +P+ + + CG D + I+ ++ CG+N++NKLG++ G SQ N + T + V L K+ +V G SH+A + + + FG N +GQ+G ++ + V + I ++CGD FT+ E++ WG+ GRLG
Sbjct: 4 YEKIKVVGRGAFGIVHLCRRRSDGAFVILKEIPVEQMSRDERLAAQNECQVLKLLNHPNIIEYYENFLEDKALMIAMEYAPGGTLAEYIQKRCNSLLDEDTILHFFVQILLALYHVHNKLILHRDLKTQNILLDKHQMIVKIGDFGISKILVSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLILNMLNLDPSKRPQLNEIMALPICTRPLLNLYTDVGNVKMRRIEKPLSAVQLGLPGRP--GGRVSTSRLREGSFGFGSGQVHSLPLSSVYTWGSGITTPFRLP--MPNTEVLQVSLGRTQKMGVTKSGRLITW---EAPSVGAGELGPPGVVEHMQLLFISRFLEGQSGVTIKSVSCGDLFTTCMTERGIIMTFGSGSNGCLGHGNFNDVTQPKIVEALLGYE--------LVQVACGASHVVAVTN-EREVFAWGRGDNGRLGLGTQDTHNSPQQVCLPV-GFEAQRAVCGVDCSMIISS-QSSIMACGSNRFNKLGLDRITAG-EEPSQVNQVEEAHSFTPVQSVP-LNTDKIVSVDIGTSHSAAVTEGGQCFTFGSNQHGQMGCSSRRSGR---VPYLVLGLQDITMVACGDAFTLAIGAEGEVYTWGKGARGRLG 650 HSP 2 Score: 55.4546 bits (132), Expect = 2.031e-7 Identity = 31/90 (34.44%), Postives = 47/90 (52.22%), Query Frame = 1
Query: 1777 VKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFA--GKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGL 2040
V + +VSCG+ T + + FG S G +G G+ DV + V A G ++ +++CG + T E+F WGR D+GRLGL
Sbjct: 397 VTIKSVSCGDLFTTCMTERGIIMTFGSGSNGCLGHGN---FNDVTQPKIVEALLGYELVQVACGASHVVAVTNEREVFAWGRGDNGRLGL 483
BLAST of NEK8-1 vs. Ensembl Medaka
Match: nek8 (NIMA related kinase 8 [Source:NCBI gene;Acc:101161178]) HSP 1 Score: 290.426 bits (742), Expect = 2.965e-86 Identity = 210/654 (32.11%), Postives = 327/654 (50.00%), Query Frame = 1
Query: 184 LYRNAVDNSLVVWKEIDIDKNKINE---IQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYL-RQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLL-KIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWK----------GNCQ-SMIDQVHRTNQANSNSRPCSGDRKERS-------------IVSLN-SEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYK-----IPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGI-NSNETEMINQFEKITVTDPIDKSPVIINQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYNKLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLG 2037
L R D + V+ KEI +++ +E QNE +L +L+HPNI+ YY +F+ + I MEYA G+L+ Y+ ++ L E +L +F Q++ AL ++H I+HRD+KT NI L ++ KIGDFGISK+L A T VGTP Y+SPE+ +G PY+ KSDIWA GCVLYE+ S K+ FE +N +L +KI++G F + + + +L L+ +L +P+KRP +++ P+ GN + I++ Q RP G R S + SL S V+ WG G ++ LQV+ G + + +T L TW + +G+LG +V I + ++ GV IK VSCG+ T C++ G + GS G LG N N+ E + + + Q++CG H A+ E +F WG G+ GRLGLG +D P V +P+ + + CG D + I+ ++ CG+N++NKLG++ G SQ N + T + V L K+ +V G SH+A + + + FG N +GQ+G ++ + V + I ++CGD FT+ E++ WG+ GRLG
Sbjct: 36 LCRRRSDGAFVILKEIPVEQMSRDERLAAQNECQVLKLLNHPNIIEYYENFLEDKALMIAMEYAPGGTLAEYIQKRCNSLLDEDTILHFFVQILLALYHVHNKLILHRDLKTQNILLDKHQMIVKIGDFGISKILVSKSKAYTVVGTPCYISPELCEGKPYNQKSDIWALGCVLYELASLKRAFEAANLPALVLKIMSGTFAPISDRYSPELRQLILNMLNLDPSKRPQLNEIMALPICTRPLLNLYTDVGNVKMRRIEKPLSAVQLGLPGRP--GGRVSTSRLREGSFGFGSGQVHSLPLSSVYTWGSGITTPFRLP--MPNTEVLQVSLGRTQKMGVTKSGRLITW---EAPSVGAGELGPPGVVEHMQLLFISRFLEGQSGVTIKSVSCGDLFTTCMTERGIIMTFGSGSNGCLGHGNFNDVTQPKIVEALLGYE--------LVQVACGASHVVAVTN-EREVFAWGRGDNGRLGLGTQDTHNSPQQVCLPV-GFEAQRAVCGVDCSMIISS-QSSIMACGSNRFNKLGLDRITAG-EEPSQVNQVEEAHSFTPVQSVP-LNTDKIVSVDIGTSHSAAVTEGGQCFTFGSNQHGQMGCSSRRSGR---VPYLVLGLQDITMVACGDAFTLAIGAEGEVYTWGKGARGRLG 666 HSP 2 Score: 55.4546 bits (132), Expect = 1.965e-7 Identity = 31/90 (34.44%), Postives = 47/90 (52.22%), Query Frame = 1
Query: 1777 VKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFA--GKKIEKISCGDYFTIVSTTNNEIFGWGRADSGRLGL 2040
V + +VSCG+ T + + FG S G +G G+ DV + V A G ++ +++CG + T E+F WGR D+GRLGL
Sbjct: 413 VTIKSVSCGDLFTTCMTERGIIMTFGSGSNGCLGHGN---FNDVTQPKIVEALLGYELVQVACGASHVVAVTNEREVFAWGRGDNGRLGL 499
BLAST of NEK8-1 vs. Ensembl Medaka
Match: nek4 (NIMA related kinase 4 [Source:NCBI gene;Acc:101169778]) HSP 1 Score: 180.259 bits (456), Expect = 1.517e-46 Identity = 100/258 (38.76%), Postives = 159/258 (61.63%), Query Frame = 1
Query: 133 SYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDID---KNKINEIQNEIDILSILDHPNIVAYYNHFVYIDT-VFIEMEYANAGSLSNYLRQLK-EDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVL-NEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWK 888
SY+ +RV+GKG+YG+ L ++ D V K++++ K + + E +LS L HPNIV Y + D ++I M + G L + L+Q K E L E V+ +F Q+ AL Y+H+ +I+HRD+KT NIFLT + ++K+GD GI++VL N+++ A+T +GTP YMSPE+ PY+ KSD+WA GC +YE+ + K F + SL +I+ G+ MP+ + L DL++ +L +P +RP + +L+ P K
Sbjct: 3 SYIFIRVVGKGSYGEVNLVKHKTDRKQYVIKKLNLSTSSKRERRSAEQEAQLLSQLRHPNIVTYRESWEGDDRQLYIVMGFCEGGDLYHRLKQRKGELLPERQVVEWFVQIAMALQYLHERNILHRDLKTQNIFLTKTNIIKVGDLGIARVLENQNDMASTLIGTPYYMSPELFSNKPYNHKSDVWALGCCVYEMSTLKHAFNAKDMNSLVYRIVEGKLPQMPSRYDPQLGDLIKSMLSKKPEERPDVKLILRQPYIK 260
BLAST of NEK8-1 vs. Ensembl Medaka
Match: ENSORLT00000000545.2 (pep primary_assembly:ASM223467v1:4:1040495:1044496:1 gene:ENSORLG00000000439.2 transcript:ENSORLT00000000545.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 166.777 bits (421), Expect = 3.705e-46 Identity = 100/252 (39.68%), Postives = 147/252 (58.33%), Query Frame = 1
Query: 136 YVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDID---KNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDL-SESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHP 879
Y V+ +G+G++GKA+L + D V KEI I + E + E+ +L+ + HPNIV Y F ++I M+Y G L + K L SE +L +F Q+ AL ++H I+HRDIK+ NIFLT +++GDFGI++VLN E A T +GTP Y+SPE+ + PY++K DIWA GCVLYE+ + K FE N +L +KII G + + + +L LL +L P +RPS +L+ P
Sbjct: 4 YENVKQIGEGSFGKAILVNSKEDGRQYVIKEIGISGMSTKERRESRKEVSVLANMSHPNIVQYKESFEG-GCLYIVMDYCEGGDLFKKINSQKGVLFSEDQILDWFVQICLALKHVHGRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNTVELARTCIGTPYYLSPEICENKPYNNK-DIWALGCVLYEMCTLKHAFEAGNMKNLVLKIIRGSYPPVSVHYSPELRSLLAQLFRRNPRERPSVSSILEKP 253
BLAST of NEK8-1 vs. Planmine SMEST
Match: SMESG000053193.1 (SMESG000053193.1) HSP 1 Score: 757.288 bits (1954), Expect = 0.000e+0 Identity = 380/382 (99.48%), Postives = 380/382 (99.48%), Query Frame = 1
Query: 67 MDNCRDNEVLSNRDFSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYKIPKL 1212
M NCRDNEVLSNRDFSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSDLLDLLQRLLLNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYKIP L
Sbjct: 1 MVNCRDNEVLSNRDFSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQNEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNEDEFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSDLLDLLQRLLLNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIVYKIPNL 382
BLAST of NEK8-1 vs. Planmine SMEST
Match: SMESG000043302.1 (SMESG000043302.1) HSP 1 Score: 309.301 bits (791), Expect = 3.947e-91 Identity = 222/646 (34.37%), Postives = 337/646 (52.17%), Query Frame = 1
Query: 109 FSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVI-INQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYN-----KLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWG 2013
FS + + ++R +GKGAYG AVLYR D+SLV+ KEI++ E Q NE+ +LS LDHPNIVAY++ F + IEMEYA+ G+L+ +L ++ L E +L FFQ++ A+ YIH+ I+HRD+KT+NIFLT ++KIGDFGISK+L+ E AN+ +GTP Y+SPE+ QG Y+ KSD+WA GC+LYE+ K FE SN +L KI+ G+F+ + + +L +L+ LL P R S E +L H + + +T +S+ + + RS+ V+V +N L + K Q+ +G +H +VLT ++ ++TW G + GQLGHG +V K P+LV SL + ++CG + +I NG + CGS G LG F+ + I+ I + IS + H + ED+++ WG + G+LGL + P ++ H +K + D T ++ + L NN +LG + + LS N ++ K+ AV+R+ V+ VS G HT VL K+ FG NS GQ+G G TK + + K KI K++ G FT+ N ++ WG
Sbjct: 42 FSIQQTINGFEKIRTVGKGAYGTAVLYRKKDDDSLVILKEINMHDLSATERQLAMNEVKVLSQLDHPNIVAYFDSFEEDGVLMIEMEYADNGTLAQFLSNQEKPLEEKKILEMFFQIVSAIHYIHEHKILHRDLKTVNIFLTKEEIIKIGDFGISKMLSTTHEGANSVIGTPYYISPEMCQGKLYNEKSDVWALGCILYEMTCLHKTFEGSNLPALVNKILKGQFEPVKGSYSDELKELISSLLEKNPDDRLSTEVILHH---------KLPMMMKTFHTDSSDNVDTAKPQTRSV------VYVMDINTLN-LSPVRLPSKIRIKQICSGENHTIVLTTDRTVFTW----GSNEF-GQLGHGDLVDRKKPELVSSLTQKSLIRIACGNDFSIFGPENGIIMTCGSGISGSLG--------HGDFKDVLKPRLIESLLTIDVVCISATNSHVVVVSS-EDVIYGWGSNKNGQLGLDSLNLYCSPQVITFKEH-VLVKNAFTSEDATMLLTETGGLLASGNNNGNKLGLNARLGFLMTMKSLSQQPTINSVHEFT-----KVEAVMRHC-VNQVSMGKGHTTVLCESGKIIAFGINSSGQLGNGSTK-NSPIPCEVKTMKKHKITKVTSGSNFTVACVDFNSLYFWG 649
BLAST of NEK8-1 vs. Planmine SMEST
Match: SMESG000043302.1 (SMESG000043302.1) HSP 1 Score: 309.301 bits (791), Expect = 4.083e-91 Identity = 222/646 (34.37%), Postives = 337/646 (52.17%), Query Frame = 1
Query: 109 FSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVI-INQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYN-----KLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWG 2013
FS + + ++R +GKGAYG AVLYR D+SLV+ KEI++ E Q NE+ +LS LDHPNIVAY++ F + IEMEYA+ G+L+ +L ++ L E +L FFQ++ A+ YIH+ I+HRD+KT+NIFLT ++KIGDFGISK+L+ E AN+ +GTP Y+SPE+ QG Y+ KSD+WA GC+LYE+ K FE SN +L KI+ G+F+ + + +L +L+ LL P R S E +L H + + +T +S+ + + RS+ V+V +N L + K Q+ +G +H +VLT ++ ++TW G + GQLGHG +V K P+LV SL + ++CG + +I NG + CGS G LG F+ + I+ I + IS + H + ED+++ WG + G+LGL + P ++ H +K + D T ++ + L NN +LG + + LS N ++ K+ AV+R+ V+ VS G HT VL K+ FG NS GQ+G G TK + + K KI K++ G FT+ N ++ WG
Sbjct: 51 FSIQQTINGFEKIRTVGKGAYGTAVLYRKKDDDSLVILKEINMHDLSATERQLAMNEVKVLSQLDHPNIVAYFDSFEEDGVLMIEMEYADNGTLAQFLSNQEKPLEEKKILEMFFQIVSAIHYIHEHKILHRDLKTVNIFLTKEEIIKIGDFGISKMLSTTHEGANSVIGTPYYISPEMCQGKLYNEKSDVWALGCILYEMTCLHKTFEGSNLPALVNKILKGQFEPVKGSYSDELKELISSLLEKNPDDRLSTEVILHHK---------LPMMMKTFHTDSSDNVDTAKPQTRSV------VYVMDINTLN-LSPVRLPSKIRIKQICSGENHTIVLTTDRTVFTW----GSNEF-GQLGHGDLVDRKKPELVSSLTQKSLIRIACGNDFSIFGPENGIIMTCGSGISGSLG--------HGDFKDVLKPRLIESLLTIDVVCISATNSHVVVVSS-EDVIYGWGSNKNGQLGLDSLNLYCSPQVITFKEH-VLVKNAFTSEDATMLLTETGGLLASGNNNGNKLGLNARLGFLMTMKSLSQQPTINSVHEFT-----KVEAVMRHC-VNQVSMGKGHTTVLCESGKIIAFGINSSGQLGNGSTK-NSPIPCEVKTMKKHKITKVTSGSNFTVACVDFNSLYFWG 658
BLAST of NEK8-1 vs. Planmine SMEST
Match: SMESG000043302.1 (SMESG000043302.1) HSP 1 Score: 309.301 bits (791), Expect = 4.204e-91 Identity = 222/646 (34.37%), Postives = 337/646 (52.17%), Query Frame = 1
Query: 109 FSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVI-INQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYN-----KLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWG 2013
FS + + ++R +GKGAYG AVLYR D+SLV+ KEI++ E Q NE+ +LS LDHPNIVAY++ F + IEMEYA+ G+L+ +L ++ L E +L FFQ++ A+ YIH+ I+HRD+KT+NIFLT ++KIGDFGISK+L+ E AN+ +GTP Y+SPE+ QG Y+ KSD+WA GC+LYE+ K FE SN +L KI+ G+F+ + + +L +L+ LL P R S E +L H + + +T +S+ + + RS+ V+V +N L + K Q+ +G +H +VLT ++ ++TW G + GQLGHG +V K P+LV SL + ++CG + +I NG + CGS G LG F+ + I+ I + IS + H + ED+++ WG + G+LGL + P ++ H +K + D T ++ + L NN +LG + + LS N ++ K+ AV+R+ V+ VS G HT VL K+ FG NS GQ+G G TK + + K KI K++ G FT+ N ++ WG
Sbjct: 46 FSIQQTINGFEKIRTVGKGAYGTAVLYRKKDDDSLVILKEINMHDLSATERQLAMNEVKVLSQLDHPNIVAYFDSFEEDGVLMIEMEYADNGTLAQFLSNQEKPLEEKKILEMFFQIVSAIHYIHEHKILHRDLKTVNIFLTKEEIIKIGDFGISKMLSTTHEGANSVIGTPYYISPEMCQGKLYNEKSDVWALGCILYEMTCLHKTFEGSNLPALVNKILKGQFEPVKGSYSDELKELISSLLEKNPDDRLSTEVILHH---------KLPMMMKTFHTDSSDNVDTAKPQTRSV------VYVMDINTLN-LSPVRLPSKIRIKQICSGENHTIVLTTDRTVFTW----GSNEF-GQLGHGDLVDRKKPELVSSLTQKSLIRIACGNDFSIFGPENGIIMTCGSGISGSLG--------HGDFKDVLKPRLIESLLTIDVVCISATNSHVVVVSS-EDVIYGWGSNKNGQLGLDSLNLYCSPQVITFKEH-VLVKNAFTSEDATMLLTETGGLLASGNNNGNKLGLNARLGFLMTMKSLSQQPTINSVHEFT-----KVEAVMRHC-VNQVSMGKGHTTVLCESGKIIAFGINSSGQLGNGSTK-NSPIPCEVKTMKKHKITKVTSGSNFTVACVDFNSLYFWG 653
BLAST of NEK8-1 vs. Planmine SMEST
Match: SMESG000043302.1 (SMESG000043302.1) HSP 1 Score: 309.301 bits (791), Expect = 4.217e-91 Identity = 222/646 (34.37%), Postives = 337/646 (52.17%), Query Frame = 1
Query: 109 FSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVVWKEIDIDKNKINEIQ---NEIDILSILDHPNIVAYYNHFVYIDTVFIEMEYANAGSLSNYLRQLKEDLSESCVLWYFFQLIYALDYIHKLHIIHRDIKTLNIFLTTSTLLKIGDFGISKVLNED-EFANTQVGTPVYMSPEVIQGLPYSSKSDIWASGCVLYEIISRKKLFEYSNHLSLAMKIINGEFQTMPNCNNSXXXXXXXXXXXNEPTKRPSAEDVLKHPVWKGNCQSMIDQVHRTNQANSNSRPCSGDRKERSIVSLNSEVFVWGGGKINALKIEQFQEKDSALQVAAGGSHFVVLTAEKDLYTWSNIQGDKKLSGQLGHGAIV-YKIPKLVQSLKGVCIKEVSCGEETTICLSTNGEMFGCGSNYYGELGINSNETEMINQFEKITVTDPIDKSPVI-INQISCGHHHAAAIGGCEDLLFTWGCGEYGRLGLGHEDNIYKPTLVDIPLHKCKLKLVYCGYDTTFIVHKCSDRLLGCGNNQYN-----KLGINIHAQGLSGSSQKNLCYDVPFETKFKIVAVLRNVKVSTVSCGNSHTAVLDIYNKLYIFGDNSYGQIGLGHTKTHKDVNKSSKVFAGKKIEKISCGDYFTIVSTTNNEIFGWG 2013
FS + + ++R +GKGAYG AVLYR D+SLV+ KEI++ E Q NE+ +LS LDHPNIVAY++ F + IEMEYA+ G+L+ +L ++ L E +L FFQ++ A+ YIH+ I+HRD+KT+NIFLT ++KIGDFGISK+L+ E AN+ +GTP Y+SPE+ QG Y+ KSD+WA GC+LYE+ K FE SN +L KI+ G+F+ + + +L +L+ LL P R S E +L H + + +T +S+ + + RS+ V+V +N L + K Q+ +G +H +VLT ++ ++TW G + GQLGHG +V K P+LV SL + ++CG + +I NG + CGS G LG F+ + I+ I + IS + H + ED+++ WG + G+LGL + P ++ H +K + D T ++ + L NN +LG + + LS N ++ K+ AV+R+ V+ VS G HT VL K+ FG NS GQ+G G TK + + K KI K++ G FT+ N ++ WG
Sbjct: 46 FSIQQTINGFEKIRTVGKGAYGTAVLYRKKDDDSLVILKEINMHDLSATERQLAMNEVKVLSQLDHPNIVAYFDSFEEDGVLMIEMEYADNGTLAQFLSNQEKPLEEKKILEMFFQIVSAIHYIHEHKILHRDLKTVNIFLTKEEIIKIGDFGISKMLSTTHEGANSVIGTPYYISPEMCQGKLYNEKSDVWALGCILYEMTCLHKTFEGSNLPALVNKILKGQFEPVKGSYSDELKELISSLLEKNPDDRLSTEVILHHK---------LPMMMKTFHTDSSDNVDTAKPQTRSV------VYVMDINTLN-LSPVRLPSKIRIKQICSGENHTIVLTTDRTVFTW----GSNEF-GQLGHGDLVDRKKPELVSSLTQKSLIRIACGNDFSIFGPENGIIMTCGSGISGSLG--------HGDFKDVLKPRLIESLLTIDVVCISATNSHVVVVSS-EDVIYGWGSNKNGQLGLDSLNLYCSPQVITFKEH-VLVKNAFTSEDATMLLTETGGLLASGNNNGNKLGLNARLGFLMTMKSLSQQPTINSVHEFT-----KVEAVMRHC-VNQVSMGKGHTTVLCESGKIIAFGINSSGQLGNGSTK-NSPIPCEVKTMKKHKITKVTSGSNFTVACVDFNSLYFWG 653 The following BLAST results are available for this feature:
BLAST of NEK8-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of NEK8-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of NEK8-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of NEK8-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of NEK8-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of NEK8-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of NEK8-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of NEK8-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of NEK8-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of NEK8-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of NEK8-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of NEK8-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of NEK8-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of NEK8-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30003066 ID=SMED30003066|Name=NEK8-1|organism=Schmidtea mediterranea sexual|type=transcript|length=2522bpback to top protein sequence of SMED30003066-orf-1 >SMED30003066-orf-1 ID=SMED30003066-orf-1|Name=SMED30003066-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=804bp MDNCRDNEVLSNRDFSTNLNVVSYVQVRVLGKGAYGKAVLYRNAVDNSLVback to top Aliases
The feature 'NEK8-1' has the following synonyms
Annotated Terms
The following terms have been associated with this transcript:
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