SMED30003036

Overview
NameSMED30003036
Smed IDSMED30003036
Length (bp)395
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003036 (SMED30003036) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003036 (SMED30003036) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003036 (SMED30003036) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003036 (SMED30003036) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30003036

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 86

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30003036h1SMcG0008881 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008881 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008881 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008881 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008787 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008787 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008787 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008787 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008824 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008824 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008824 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008824 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008880 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008880 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008880 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008880 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008883 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008883 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008883 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008883 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008893 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008893 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008893 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008893 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008788 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008788 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008788 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008788 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008881 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008881 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008881 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008881 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008787 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008787 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008787 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008787 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008824 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008824 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008824 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008824 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008880 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008880 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008880 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008880 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008883 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008883 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008883 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008883 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008893 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008893 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008893 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008893 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
pharynxSMED30003036h1SMcG0008788 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
gutSMED30003036h1SMcG0008788 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
epidermisSMED30003036h1SMcG0008788 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		whole organism asexual adult colorimetric in situ hybridization evidence
intestinal phagocyteSMED30003036h1SMcG0008788 Contig1645GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
head regionSMED30003036h1SMcG0008788 SmedASXL_078386SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30003036 SmedASXL_078270SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30003036 SmedASXL_078391SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30003036h1SMcG0008788 SmedASXL_078386SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30003036 SmedASXL_078270SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
head regionSMED30003036 SmedASXL_078391SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig14133newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig14133uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig14877uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig14877newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig20188newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig20188uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig46004newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig46004uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008788 Contig5395newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008788 Contig5395uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0009013 Contig5395newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0009013 Contig5395uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig14133newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig14133uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig14877uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig14877newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig20188newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig20188uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig46004newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008787 Contig46004uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008788 Contig5395newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0008788 Contig5395uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0009013 Contig5395newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30003036h1SMcG0009013 Contig5395uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30003036 vs. Planmine SMEST
Match: SMESG000076371.1 (SMESG000076371.1)

HSP 1 Score: 160.999 bits (406), Expect = 2.406e-49
Identity = 98/103 (95.15%), Postives = 101/103 (98.06%), Query Frame = 1 
Query:   52 WTNAMKKAKGKGFXXXXXXXSDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTXXXXXXXXXXXKCIKLMHQYQLNTNTQNKARE 360
            WTNAMKKAK KGF+EIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMH+YQLNTNTQNK +E
Sbjct:  243 WTNAMKKAKAKGFLEIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMHKYQLNTNTQNKEKE 345
[Close]
BLAST of SMED30003036 vs. Planmine SMEST
Match: SMESG000076371.1 (SMESG000076371.1)

HSP 1 Score: 160.999 bits (406), Expect = 3.310e-49
Identity = 98/103 (95.15%), Postives = 101/103 (98.06%), Query Frame = 1 
Query:   52 WTNAMKKAKGKGFXXXXXXXSDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTXXXXXXXXXXXKCIKLMHQYQLNTNTQNKARE 360
            WTNAMKKAK KGF+EIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMH+YQLNTNTQNK +E
Sbjct:  252 WTNAMKKAKAKGFLEIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMHKYQLNTNTQNKEKE 354
[Close]
BLAST of SMED30003036 vs. Planmine SMEST
Match: SMESG000076371.1 (SMESG000076371.1)

HSP 1 Score: 160.614 bits (405), Expect = 7.146e-49
Identity = 98/103 (95.15%), Postives = 101/103 (98.06%), Query Frame = 1 
Query:   52 WTNAMKKAKGKGFXXXXXXXSDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTXXXXXXXXXXXKCIKLMHQYQLNTNTQNKARE 360
            WTNAMKKAK KGF+EIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMH+YQLNTNTQNK +E
Sbjct:  276 WTNAMKKAKAKGFLEIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMHKYQLNTNTQNKEKE 378
[Close]
BLAST of SMED30003036 vs. Planmine SMEST
Match: SMESG000076371.1 (SMESG000076371.1)

HSP 1 Score: 160.614 bits (405), Expect = 8.515e-49
Identity = 98/103 (95.15%), Postives = 101/103 (98.06%), Query Frame = 1 
Query:   52 WTNAMKKAKGKGFXXXXXXXSDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTXXXXXXXXXXXKCIKLMHQYQLNTNTQNKARE 360
            WTNAMKKAK KGF+EIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMH+YQLNTNTQNK +E
Sbjct:  285 WTNAMKKAKAKGFLEIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMHKYQLNTNTQNKEKE 387
[Close]
BLAST of SMED30003036 vs. Planmine SMEST
Match: SMESG000076375.1 (SMESG000076375.1)

HSP 1 Score: 159.844 bits (403), Expect = 1.014e-48
Identity = 97/100 (97.00%), Postives = 99/100 (99.00%), Query Frame = 1 
Query:   52 WTNAMKKAKGKGFXXXXXXXSDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTXXXXXXXXXXXKCIKLMHQYQLNTNTQNK 351
            WTNAMKKAK KGF+EIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMHQY+LNTNTQNK
Sbjct:  168 WTNAMKKAKAKGFLEIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYILDHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMHQYKLNTNTQNK 267
The following BLAST results are available for this feature:
BLAST of SMED30003036 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30003036 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000076371.12.406e-4995.15SMESG000076371.1[more]
SMESG000076371.13.310e-4995.15SMESG000076371.1[more]
SMESG000076371.17.146e-4995.15SMESG000076371.1[more]
SMESG000076371.18.515e-4995.15SMESG000076371.1[more]
SMESG000076375.11.014e-4897.00SMESG000076375.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003036 ID=SMED30003036|Name=SMED30003036|organism=Schmidtea mediterranea sexual|type=transcript|length=395bp
TTATTATTTTTAATATTATTTGTATTGTTATTTCAATATTTGTATTAGAT
CTGGACTAATGCCATGAAAAAAGCTAAGGGAAAGGGATTCATTGAAATCG
AAGAAGAAATATCAGATAGAATGATTCTGTTGGAATCAAGGGAAAAGGTC
AATTTCACATTACTGCACTCGGCAATCTGCATTAATGACCTTGACATGAT
TAAATATATACTCGATCATCAAAATATTATTGACCTTAATTCCAATAATG
AAATATTTGGAACGGCATTGGCATTAGCTCATTCCCTTCATCTCTCAAAA
TGTATAAAACTAATGCACCAATACCAACTCAATACAAATACACAGAACAA
GGCAAGAGAATAATTGATTTTATTGGAGTAATATTGATATTATTT
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protein sequence of SMED30003036-orf-1

>SMED30003036-orf-1 ID=SMED30003036-orf-1|Name=SMED30003036-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=100bp
MKKAKGKGFIEIEEEISDRMILLESREKVNFTLLHSAICINDLDMIKYIL
DHQNIIDLNSNNEIFGTALALAHSLHLSKCIKLMHQYQLNTNTQNKARE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000026gut
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036770Ankyrin_rpt-contain_sf
IPR020683Ankyrin_rpt-contain_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036770Ankyrin repeat-containing domain superfamilyGENE3DG3DSA:1.25.40.20coord: 1..99
e-value: 7.4E-8
score: 34.0
IPR036770Ankyrin repeat-containing domain superfamilySUPERFAMILYSSF48403Ankyrin repeatcoord: 8..96