SMED30003032

Overview
NameSMED30003032
Smed IDSMED30003032
Length (bp)2676
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30003032 (SMED30003032) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30003032 (SMED30003032) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30003032 (SMED30003032) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30003032 (SMED30003032) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30003032

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30003032SMESG000061110.1 Contig2130newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003032SMESG000061110.1 Contig2130uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003032SMESG000066535.1 Contig2130newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30003032SMESG000066535.1 Contig2130uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
headSMED30003032SMESG000061110.1 SmedASXL_016675SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30003032SMESG000061110.1 dd_Smed_v4_11638_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
epidermal cellSMED30003032SMESG000061110.1 dd_Smed_v4_11638_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
headSMED30003032SMESG000061110.1 dd_Smed_v6_11638_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30003032SMESG000061110.1 dd_Smed_v4_11638_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30003032SMESG000061110.1 dd_Smed_v4_11638_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30003032 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001521:29814..45303 -12950
Homology
BLAST of SMED30003032 vs. Planmine SMEST
Match: SMESG000061110.1 (SMESG000061110.1)

HSP 1 Score: 1300.8 bits (3365), Expect = 0.000e+0
Identity = 819/838 (97.73%), Postives = 824/838 (98.33%), Query Frame = 1
Query:  100 MSAYERRLLRKEINRKLYIMSNKESQENDFLNSSQLITERQHMHMIITKHFREQENKRTSKKRIPFPIESTSLPHVXXXXXXXXXXXXKAITSKSEEVNKVLDAHSYRTATPYYTNPELTVERGNFMNYSQHVGTLLHTPRYIEFLXXXXXXXXXXXXXXXXXDFVDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLENKYIQNTENYHXXXXXXXXXXXXXNMSISNAESSTFSNDXXXXXXXXXXXXXXXGNHLKDSSINEGRINSSRMSREKRTNNSRNFGLSNKSNNDTISQTSVKSEKEDLHPDLSFHXXXXXXXXXXXXXXXXXXXXXRKSIVSKASNERIDSSNETELSTDLTTPDKGNQSPYDKNVASKEIEQGEDLKENVTGAYENDETSNQTKADLNKSDQNMSPNTEKSTIKRSNFEEPDKFEQEVYFSQETKNGLSITNKETNLCPKKMERTEVLCSDDEMFHFEMDDKSKTNFMSKMEEFLNNNDFEPDEVHLMKSYFETIFRFQITNLKYVKDYYKFSAKHNKGSFITFAKICNKHPVHSLNQDEVRRITDLIEKNSIINSADKKNESRIKNKSVDILEIPNGENAEEINSRFESSQNDKIQNDMINKDTNNDXXXXXXXXXXXXXXXXXXXXXXXVTKDEDEDFSEAFEKLFRYSVAEISKEDLEAIKRLAENYKESKNDFNDMDNNSEVLLNDHEQLQENSVSNCNDIQKDEKLVEHVSRKYSKDDIKEQNETTNNETVIKNEFIEHKETDSERVNEEIKSTXXXXXXXXXXXXXXXXRNFRNLFRTSIDNIKDKDLEKVNQIIEAHEKQYKYNTTIIQ 2613
            MSAYERRLLRKEINRKLYIMSNKESQENDFLNSSQLITERQHM+MIITKHFREQENKRTSKKRIPFPIESTSLPHVNLGRTNRLLNLTKAITSKSEEVNKVLDAHSYRTATPYYTNPELTVERGNFMNYSQHVGTLLHTPRYIEFLNSFRESKSSNSEKRSNNDFVDSVSCSSLDHSSNSKKT+RSSQSSNSSTSSNKTLENKYIQNTENYHSVSLSRELSS SLNMSISNAESSTFSND KVPSKRSSSVLSPSGNHLKDSSINEGRINSSRMSR KRTNNSRNFGLSNKSNNDTISQTSVKSEKEDLHPDLSFHNKNEIVNDVNNVEEDFINNDNRKSIVSKASNERIDSSNETELSTDLT PDKGNQSPYDKNVASKEIEQGEDLKENVTGAYENDETSNQTKADLNKSDQNMSPNTEKSTIKRSNFEEPDKFEQEV+FSQETKNGLSITNKETNL PKKMERTEVLCSDDEMFHFEMDDK KTNFMSKMEEFLNNNDFEPDEVHLMKSYFETIFRFQ TNLKYVKDYYKFSAKHNKGSFITFAKICNKHPVHSLNQDEVRRITDLIEKNSIINSADKKNESRIKNKSVDILEIPNGENAEEINSRFESSQNDKIQNDMINKDTNNDEEVKSEKGENKE ENENLCFEENVTKDEDEDFSEAFEKLFRYS+AEISKEDLEAIKRLAENYKESKNDFNDMDNNSEVL NDHEQ QENSVSNC DIQKDEKLVEHVSRKYSKDDIKEQNETTNNET IKNEFIEHKETDSE VNEEIK TLLEDNNIESEEENEILRNFRNLFRTSIDNIKD+DLEKVNQIIEAHEKQYKYNTTIIQ
Sbjct:    1 MSAYERRLLRKEINRKLYIMSNKESQENDFLNSSQLITERQHMYMIITKHFREQENKRTSKKRIPFPIESTSLPHVNLGRTNRLLNLTKAITSKSEEVNKVLDAHSYRTATPYYTNPELTVERGNFMNYSQHVGTLLHTPRYIEFLNSFRESKSSNSEKRSNNDFVDSVSCSSLDHSSNSKKTYRSSQSSNSSTSSNKTLENKYIQNTENYHSVSLSRELSSTSLNMSISNAESSTFSNDIKVPSKRSSSVLSPSGNHLKDSSINEGRINSSRMSRGKRTNNSRNFGLSNKSNNDTISQTSVKSEKEDLHPDLSFHNKNEIVNDVNNVEEDFINNDNRKSIVSKASNERIDSSNETELSTDLTRPDKGNQSPYDKNVASKEIEQGEDLKENVTGAYENDETSNQTKADLNKSDQNMSPNTEKSTIKRSNFEEPDKFEQEVFFSQETKNGLSITNKETNLSPKKMERTEVLCSDDEMFHFEMDDKGKTNFMSKMEEFLNNNDFEPDEVHLMKSYFETIFRFQTTNLKYVKDYYKFSAKHNKGSFITFAKICNKHPVHSLNQDEVRRITDLIEKNSIINSADKKNESRIKNKSVDILEIPNGENAEEINSRFESSQNDKIQNDMINKDTNNDEEVKSEKGENKEIENENLCFEENVTKDEDEDFSEAFEKLFRYSIAEISKEDLEAIKRLAENYKESKNDFNDMDNNSEVLFNDHEQPQENSVSNCYDIQKDEKLVEHVSRKYSKDDIKEQNETTNNETFIKNEFIEHKETDSESVNEEIKLTLLEDNNIESEEENEILRNFRNLFRTSIDNIKDEDLEKVNQIIEAHEKQYKYNTTIIQ 838          
The following BLAST results are available for this feature:
BLAST of SMED30003032 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30003032 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000061110.10.000e+097.73SMESG000061110.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30003032 ID=SMED30003032|Name=SMED30003032|organism=Schmidtea mediterranea sexual|type=transcript|length=2676bp
TGAAGATATATTTAAATATTTTAAAAACATTATTATATTTAAAATTAATT
TAATATTAAAATTTTCACTTAGTCGAAATAAAATAAATTTTTTGTCATCA
TGAGTGCCTACGAACGTCGGCTATTACGAAAAGAAATCAATCGTAAACTA
TATATAATGTCCAACAAAGAATCTCAAGAAAATGACTTTCTAAATTCATC
GCAGTTGATCACTGAACGGCAGCATATGCACATGATAATTACGAAACACT
TTAGAGAACAGGAAAACAAAAGAACTTCAAAAAAAAGGATTCCGTTTCCT
ATCGAAAGCACATCCTTGCCCCATGTTAATTTAGGTCGCACCAATCGTTT
ACTTAATCTAACAAAAGCAATTACTAGTAAATCTGAAGAAGTCAACAAAG
TTCTCGATGCGCATTCGTACAGGACTGCTACTCCGTACTACACGAATCCA
GAATTGACTGTCGAGAGGGGTAATTTCATGAACTACTCACAACACGTTGG
CACGTTATTGCACACCCCCCGTTATATTGAATTCCTCAACAGTTTTCGTG
AATCAAAATCTTCAAACTCCGAAAAAAGATCAAATAACGATTTTGTAGAC
TCTGTTTCATGTTCTTCATTGGATCATTCTTCTAATTCCAAGAAAACATT
TAGGAGCTCCCAATCCTCCAATTCTTCAACTTCTTCAAATAAAACATTGG
AAAACAAATATATTCAAAATACCGAAAACTATCATTCGGTCTCATTGAGT
AGAGAATTATCCAGCGCTTCTTTAAACATGTCCATCAGTAATGCAGAATC
GTCAACCTTCTCAAATGATAAAAAGGTTCCTTCAAAAAGATCAAGTTCTG
TATTATCTCCTTCAGGAAATCATTTAAAAGATTCTTCTATTAATGAAGGA
CGAATTAATTCGTCAAGAATGTCTCGTGAAAAAAGAACTAATAATTCTAG
AAATTTTGGTCTTTCAAATAAGAGTAACAATGATACTATATCTCAAACGT
CGGTCAAATCTGAAAAAGAAGACTTACATCCTGATTTAAGCTTTCATAAT
AAGAATGAAATCGTCAATGATGTGAATAATGTTGAAGAAGATTTCATTAA
CAATGACAATAGAAAGTCAATAGTTTCAAAAGCATCAAATGAAAGAATAG
ATTCATCTAATGAAACTGAATTATCAACAGATCTTACAACACCTGATAAA
GGGAACCAAAGTCCATACGACAAGAATGTAGCATCTAAAGAAATAGAACA
AGGCGAGGATTTAAAAGAAAATGTTACAGGAGCATATGAGAATGATGAGA
CAAGCAACCAAACAAAAGCTGATTTAAACAAAAGTGATCAAAACATGTCA
CCAAACACTGAAAAAAGCACTATAAAGCGAAGTAATTTTGAAGAACCAGA
TAAATTTGAACAAGAAGTTTATTTCTCACAGGAAACTAAAAACGGGTTAT
CAATAACAAATAAGGAAACTAATTTATGTCCAAAAAAAATGGAAAGAACT
GAAGTATTATGCTCTGATGATGAAATGTTCCATTTCGAAATGGATGACAA
GAGCAAAACAAATTTTATGTCAAAAATGGAAGAATTCCTAAACAATAATG
ATTTTGAACCTGACGAAGTTCATCTGATGAAAAGCTACTTTGAAACCATT
TTTCGTTTCCAAATCACTAATCTGAAATATGTTAAAGATTATTATAAGTT
CTCTGCAAAACATAATAAAGGGAGTTTCATAACTTTCGCCAAGATTTGTA
ACAAACATCCAGTTCATAGTCTCAATCAGGATGAAGTAAGGAGAATAACC
GATCTTATTGAAAAGAATTCCATCATAAACTCAGCCGATAAAAAGAATGA
AAGTCGAATAAAAAATAAATCAGTAGATATTTTGGAAATACCCAATGGAG
AAAATGCTGAAGAAATTAATTCTCGATTTGAATCTTCCCAAAACGATAAA
ATTCAAAATGACATGATAAATAAAGACACAAATAATGATGAAGAAGTTAA
AAGTGAAAAAGGAGAGAATAAAGAAAATGAAAACGAAAACCTTTGTTTTG
AAGAGAATGTAACAAAAGACGAAGATGAAGATTTTTCGGAAGCTTTTGAA
AAATTATTTCGATACTCCGTTGCGGAGATTTCAAAAGAAGATTTAGAAGC
TATTAAGAGATTGGCTGAAAATTATAAAGAAAGCAAAAATGATTTTAATG
ATATGGATAATAACAGTGAGGTATTATTAAATGACCATGAGCAGCTTCAG
GAGAATTCGGTTTCCAATTGTAATGACATCCAAAAAGATGAAAAATTAGT
TGAACATGTTTCTAGAAAATATTCAAAAGATGACATAAAAGAGCAAAATG
AGACTACAAACAATGAGACTGTCATAAAAAATGAATTTATAGAACACAAG
GAAACGGATTCAGAAAGAGTAAATGAAGAAATCAAGTCGACTTTGTTAGA
AGACAACAACATTGAATCAGAAGAAGAAAATGAAATACTGAGGAATTTTA
GGAATCTATTTCGTACTTCTATAGATAACATTAAAGACAAAGATTTAGAA
AAGGTTAATCAAATAATTGAGGCCCATGAAAAACAATACAAATATAATAC
AACAATTATACAATAACTTTTCGCTCAAACTAACTAATTATTATACTTGT
GATACTTACAATCTGCATTGAACGCT
back to top

protein sequence of SMED30003032-orf-1

>SMED30003032-orf-1 ID=SMED30003032-orf-1|Name=SMED30003032-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=839bp
MSAYERRLLRKEINRKLYIMSNKESQENDFLNSSQLITERQHMHMIITKH
FREQENKRTSKKRIPFPIESTSLPHVNLGRTNRLLNLTKAITSKSEEVNK
VLDAHSYRTATPYYTNPELTVERGNFMNYSQHVGTLLHTPRYIEFLNSFR
ESKSSNSEKRSNNDFVDSVSCSSLDHSSNSKKTFRSSQSSNSSTSSNKTL
ENKYIQNTENYHSVSLSRELSSASLNMSISNAESSTFSNDKKVPSKRSSS
VLSPSGNHLKDSSINEGRINSSRMSREKRTNNSRNFGLSNKSNNDTISQT
SVKSEKEDLHPDLSFHNKNEIVNDVNNVEEDFINNDNRKSIVSKASNERI
DSSNETELSTDLTTPDKGNQSPYDKNVASKEIEQGEDLKENVTGAYENDE
TSNQTKADLNKSDQNMSPNTEKSTIKRSNFEEPDKFEQEVYFSQETKNGL
SITNKETNLCPKKMERTEVLCSDDEMFHFEMDDKSKTNFMSKMEEFLNNN
DFEPDEVHLMKSYFETIFRFQITNLKYVKDYYKFSAKHNKGSFITFAKIC
NKHPVHSLNQDEVRRITDLIEKNSIINSADKKNESRIKNKSVDILEIPNG
ENAEEINSRFESSQNDKIQNDMINKDTNNDEEVKSEKGENKENENENLCF
EENVTKDEDEDFSEAFEKLFRYSVAEISKEDLEAIKRLAENYKESKNDFN
DMDNNSEVLLNDHEQLQENSVSNCNDIQKDEKLVEHVSRKYSKDDIKEQN
ETTNNETVIKNEFIEHKETDSERVNEEIKSTLLEDNNIESEEENEILRNF
RNLFRTSIDNIKDKDLEKVNQIIEAHEKQYKYNTTIIQ*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 675..705
NoneNo IPR availableCOILSCoilCoilcoord: 633..660
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..315
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 344..430
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 170..200
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 379..394
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 301..315
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 356..377
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..300
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 395..424