SMED30002942

Overview
NameSMED30002942
Smed IDSMED30002942
Length (bp)2734
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002942 (SMED30002942) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002942 (SMED30002942) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002942 (SMED30002942) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002942 (SMED30002942) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002942

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30002942SMESG000009948.1 SmedASXL_002930SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
neoblastSMED30002942SMESG000009948.1 dd_Smed_v4_21084_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002942SMESG000009948.1 Contig40637newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002942SMESG000009948.1 Contig40637uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
zeta neoblastSMED30002942SMESG000009948.1 dd_Smed_v4_21084_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30002942 vs. Planmine SMEST
Match: SMESG000009948.1 (SMESG000009948.1)

HSP 1 Score: 1092.03 bits (2823), Expect = 0.000e+0
Identity = 607/611 (99.35%), Postives = 609/611 (99.67%), Query Frame = 3 
Query:   81 MTMNDANIKCLLHCSENILQCDVKHVSDFRDITLFFKILNNKPXXXXXXXXXXXXXXXXXXDIEDVLNLDDKNFLTDFELLKILLLCIVVYILTNNDKGGSKKLNNLCDNIPIDLHKNACMMFAPLIVKFSDKIEITNKDLENALLLNDKENQTNFFSPIRDKHRPYLVNEKNISVHKQKIYRDMKASQSFEPFPLEMCTPPETSSPLVKLVEYVQSPNCQEKIDLRKTNKNLEKNVNQLEHQLFMNSNFENELDKTIVVWKQKCKNLEKEVKSMQTFIDEKDDIIREKSKEIDELKKVACQNKKLEDKSKDYQEKINEIQILNAQLDEKCEEIRNLKFENRESSHFKALAIDRRTVIKSLTGQIEQYKLMETQFHETCSLLMKTQSELNELQNQIRSSQMNKXXXXXXXXXXXXXIYNPESLAGIVEMERDXXXXXXXXXXXXXXXXQSYENAMEKISELTRALNDSNVQCSQLRLEHESIATFCQSMNNCANQSSDLDSSKCLNDSIQEIFRQIQTTVTNQKENIEHLITANSILTNDINHKVDIEKKLILEIDSKTKHFXXXXXXXXXXXXXXXPDKTFDIKDKEFFLEMKYKIISYQQTTENLKNHT 1913
            MTMNDANIKCLLHCSENILQCDVKHVSDFRDITLFFKILNNKPIELDIENLLINLSNIFSFDIEDVLNLDDKNFLTDFELLKILLLCIVVYILTNNDKGGSKKLNNLCDNIPIDLHKNACMMFAPLIVKFSDKIEITNKDLENALLLNDKENQTNFFSPIRDKHRPYLVNEKNISVHKQKIYRDMKASQSFEPFPLEMCTPPETSSPLVKLVEYVQSPNCQEKIDLRKTNKNLEKNVNQLEHQLFMNSNFENELDKTIVVWKQKCKNLEKEVKSMQTFIDEKDDIIREKSKEIDELKKVACQNKKLEDKSKDYQEKINEIQILNAQLDEKCEEIRNLKFENRESSHFKALAIDRRTVIKSLTGQIEQYKLMETQFHETCSLLMKTQSELNELQNQIRSSQMNKNSDSFSEVSFNSSIYNPESLAGIVEMERDELKTELSLLKESSVTKQSYENAMEKISELTRALNDSNVQCSQLR EHESIATFCQSMNNCANQSSDLDSSKCLNDSIQ IFRQI+TTVTNQKENIEHLITANSILTNDINHKVDIEKKLILEIDSKTKHFENIELEL+EIKELLLPDKTFDIKDKEFFLEMKYKIISYQQTTENLKNHT
Sbjct:    1 MTMNDANIKCLLHCSENILQCDVKHVSDFRDITLFFKILNNKPIELDIENLLINLSNIFSFDIEDVLNLDDKNFLTDFELLKILLLCIVVYILTNNDKGGSKKLNNLCDNIPIDLHKNACMMFAPLIVKFSDKIEITNKDLENALLLNDKENQTNFFSPIRDKHRPYLVNEKNISVHKQKIYRDMKASQSFEPFPLEMCTPPETSSPLVKLVEYVQSPNCQEKIDLRKTNKNLEKNVNQLEHQLFMNSNFENELDKTIVVWKQKCKNLEKEVKSMQTFIDEKDDIIREKSKEIDELKKVACQNKKLEDKSKDYQEKINEIQILNAQLDEKCEEIRNLKFENRESSHFKALAIDRRTVIKSLTGQIEQYKLMETQFHETCSLLMKTQSELNELQNQIRSSQMNKNSDSFSEVSFNSSIYNPESLAGIVEMERDELKTELSLLKESSVTKQSYENAMEKISELTRALNDSNVQCSQLRSEHESIATFCQSMNNCANQSSDLDSSKCLNDSIQVIFRQIKTTVTNQKENIEHLITANSILTNDINHKVDIEKKLILEIDSKTKHFENIELELKEIKELLLPDKTFDIKDKEFFLEMKYKIISYQQTTENLKNHT 611

HSP 2 Score: 401.749 bits (1031), Expect = 2.048e-119
Identity = 223/232 (96.12%), Postives = 227/232 (97.84%), Query Frame = 2 
Query: 1913 LNNYRILRNEIATVKQIFVNIIQSLSVDFQNFQFLILSDSPSKHSFSNPFIESLHGLYNTLKENQANQDQLYSDTQELI-SLEKSQIKSEKRIKEHERMQAELTVKHKNEQLEMKTELSKLIFEYQECLVNLEDISSKYQEQTELKNENENIEKRISELTMQIDKINXXXXXXXXXXXXXXQEIKLINMNYSKEIENLKSWNATYKLKSEKNDAEKHEKIKNLETKIDTKQR 2605
            LNNYRILRNEIATVKQIFVNI QSLSVD Q+FQFLILSDSPSKHSFSNPFIESLHGLYN+LKENQANQDQLYSDTQELI SLEKSQI+SEKRIKEHERMQAELTVKHKNEQLEMKTELSKLIFEYQECLVNLEDISSKYQEQTELKNENENI KRI ELTMQIDKINEELSTSESKLLSKTQEIKLINMNYSKEIENLKSWNATYKLKSEKN+AEKHEKIKNLETKIDTKQR
Sbjct:  612 LNNYRILRNEIATVKQIFVNITQSLSVDLQDFQFLILSDSPSKHSFSNPFIESLHGLYNSLKENQANQDQLYSDTQELIKSLEKSQIESEKRIKEHERMQAELTVKHKNEQLEMKTELSKLIFEYQECLVNLEDISSKYQEQTELKNENENIVKRILELTMQIDKINEELSTSESKLLSKTQEIKLINMNYSKEIENLKSWNATYKLKSEKNEAEKHEKIKNLETKIDTKQR 843
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BLAST of SMED30002942 vs. Planmine SMEST
Match: SMESG000009948.1 (SMESG000009948.1)

HSP 1 Score: 1087.79 bits (2812), Expect = 0.000e+0
Identity = 605/609 (99.34%), Postives = 607/609 (99.67%), Query Frame = 3 
Query:   87 MNDANIKCLLHCSENILQCDVKHVSDFRDITLFFKILNNKPXXXXXXXXXXXXXXXXXXDIEDVLNLDDKNFLTDFELLKILLLCIVVYILTNNDKGGSKKLNNLCDNIPIDLHKNACMMFAPLIVKFSDKIEITNKDLENALLLNDKENQTNFFSPIRDKHRPYLVNEKNISVHKQKIYRDMKASQSFEPFPLEMCTPPETSSPLVKLVEYVQSPNCQEKIDLRKTNKNLEKNVNQLEHQLFMNSNFENELDKTIVVWKQKCKNLEKEVKSMQTFIDEKDDIIREKSKEIDELKKVACQNKKLEDKSKDYQEKINEIQILNAQLDEKCEEIRNLKFENRESSHFKALAIDRRTVIKSLTGQIEQYKLMETQFHETCSLLMKTQSELNELQNQIRSSQMNKXXXXXXXXXXXXXIYNPESLAGIVEMERDXXXXXXXXXXXXXXXXQSYENAMEKISELTRALNDSNVQCSQLRLEHESIATFCQSMNNCANQSSDLDSSKCLNDSIQEIFRQIQTTVTNQKENIEHLITANSILTNDINHKVDIEKKLILEIDSKTKHFXXXXXXXXXXXXXXXPDKTFDIKDKEFFLEMKYKIISYQQTTENLKNHT 1913
            MNDANIKCLLHCSENILQCDVKHVSDFRDITLFFKILNNKPIELDIENLLINLSNIFSFDIEDVLNLDDKNFLTDFELLKILLLCIVVYILTNNDKGGSKKLNNLCDNIPIDLHKNACMMFAPLIVKFSDKIEITNKDLENALLLNDKENQTNFFSPIRDKHRPYLVNEKNISVHKQKIYRDMKASQSFEPFPLEMCTPPETSSPLVKLVEYVQSPNCQEKIDLRKTNKNLEKNVNQLEHQLFMNSNFENELDKTIVVWKQKCKNLEKEVKSMQTFIDEKDDIIREKSKEIDELKKVACQNKKLEDKSKDYQEKINEIQILNAQLDEKCEEIRNLKFENRESSHFKALAIDRRTVIKSLTGQIEQYKLMETQFHETCSLLMKTQSELNELQNQIRSSQMNKNSDSFSEVSFNSSIYNPESLAGIVEMERDELKTELSLLKESSVTKQSYENAMEKISELTRALNDSNVQCSQLR EHESIATFCQSMNNCANQSSDLDSSKCLNDSIQ IFRQI+TTVTNQKENIEHLITANSILTNDINHKVDIEKKLILEIDSKTKHFENIELEL+EIKELLLPDKTFDIKDKEFFLEMKYKIISYQQTTENLKNHT
Sbjct:    1 MNDANIKCLLHCSENILQCDVKHVSDFRDITLFFKILNNKPIELDIENLLINLSNIFSFDIEDVLNLDDKNFLTDFELLKILLLCIVVYILTNNDKGGSKKLNNLCDNIPIDLHKNACMMFAPLIVKFSDKIEITNKDLENALLLNDKENQTNFFSPIRDKHRPYLVNEKNISVHKQKIYRDMKASQSFEPFPLEMCTPPETSSPLVKLVEYVQSPNCQEKIDLRKTNKNLEKNVNQLEHQLFMNSNFENELDKTIVVWKQKCKNLEKEVKSMQTFIDEKDDIIREKSKEIDELKKVACQNKKLEDKSKDYQEKINEIQILNAQLDEKCEEIRNLKFENRESSHFKALAIDRRTVIKSLTGQIEQYKLMETQFHETCSLLMKTQSELNELQNQIRSSQMNKNSDSFSEVSFNSSIYNPESLAGIVEMERDELKTELSLLKESSVTKQSYENAMEKISELTRALNDSNVQCSQLRSEHESIATFCQSMNNCANQSSDLDSSKCLNDSIQVIFRQIKTTVTNQKENIEHLITANSILTNDINHKVDIEKKLILEIDSKTKHFENIELELKEIKELLLPDKTFDIKDKEFFLEMKYKIISYQQTTENLKNHT 609

HSP 2 Score: 401.749 bits (1031), Expect = 2.043e-119
Identity = 223/232 (96.12%), Postives = 227/232 (97.84%), Query Frame = 2 
Query: 1913 LNNYRILRNEIATVKQIFVNIIQSLSVDFQNFQFLILSDSPSKHSFSNPFIESLHGLYNTLKENQANQDQLYSDTQELI-SLEKSQIKSEKRIKEHERMQAELTVKHKNEQLEMKTELSKLIFEYQECLVNLEDISSKYQEQTELKNENENIEKRISELTMQIDKINXXXXXXXXXXXXXXQEIKLINMNYSKEIENLKSWNATYKLKSEKNDAEKHEKIKNLETKIDTKQR 2605
            LNNYRILRNEIATVKQIFVNI QSLSVD Q+FQFLILSDSPSKHSFSNPFIESLHGLYN+LKENQANQDQLYSDTQELI SLEKSQI+SEKRIKEHERMQAELTVKHKNEQLEMKTELSKLIFEYQECLVNLEDISSKYQEQTELKNENENI KRI ELTMQIDKINEELSTSESKLLSKTQEIKLINMNYSKEIENLKSWNATYKLKSEKN+AEKHEKIKNLETKIDTKQR
Sbjct:  610 LNNYRILRNEIATVKQIFVNITQSLSVDLQDFQFLILSDSPSKHSFSNPFIESLHGLYNSLKENQANQDQLYSDTQELIKSLEKSQIESEKRIKEHERMQAELTVKHKNEQLEMKTELSKLIFEYQECLVNLEDISSKYQEQTELKNENENIVKRILELTMQIDKINEELSTSESKLLSKTQEIKLINMNYSKEIENLKSWNATYKLKSEKNEAEKHEKIKNLETKIDTKQR 841
The following BLAST results are available for this feature:
BLAST of SMED30002942 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002942 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000009948.12.048e-11999.35SMESG000009948.1[more]
SMESG000009948.12.043e-11999.34SMESG000009948.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002942 ID=SMED30002942|Name=SMED30002942|organism=Schmidtea mediterranea sexual|type=transcript|length=2734bp
TTTGATTTTGATAGCTTCTTAAAGAAATTTTCTATATTGCTAAATTATAA
TAACAAGTTATAATTTTCCGGCATTAAAAAATGACAATGAATGATGCCAA
TATCAAATGCCTATTGCACTGTTCTGAAAATATATTGCAATGCGACGTGA
AACATGTATCCGATTTCAGAGATATAACGTTATTTTTCAAGATTCTTAAC
AATAAACCTATAGAACTTGATATTGAAAATTTATTAATAAATCTGTCAAA
TATATTCAGTTTCGATATTGAGGATGTATTGAATTTAGATGATAAGAACT
TTTTAACTGATTTTGAATTATTAAAAATTTTGCTATTATGCATTGTTGTT
TACATTTTAACAAACAACGACAAAGGTGGCTCAAAAAAATTAAACAATTT
ATGCGACAATATCCCAATTGATTTGCACAAAAATGCCTGCATGATGTTTG
CACCTTTAATAGTGAAATTTAGTGATAAAATAGAAATCACAAATAAAGAT
TTAGAAAATGCTTTATTGTTAAATGACAAAGAAAACCAAACGAATTTTTT
CTCTCCAATTCGTGATAAACATCGACCTTATTTGGTTAACGAGAAAAACA
TTTCCGTCCATAAGCAGAAAATATACCGAGACATGAAGGCGTCTCAGTCA
TTTGAACCGTTTCCGTTAGAAATGTGCACCCCACCCGAAACGAGTTCACC
TCTAGTGAAATTGGTTGAATATGTTCAATCACCGAATTGTCAGGAAAAAA
TAGATCTGAGAAAAACCAATAAAAATCTAGAGAAAAATGTCAATCAATTA
GAGCATCAATTGTTTATGAACTCGAATTTCGAAAACGAACTCGATAAAAC
AATTGTTGTGTGGAAACAGAAATGCAAGAATCTAGAAAAGGAAGTCAAAT
CGATGCAGACATTTATCGATGAAAAAGACGATATAATTCGAGAAAAATCC
AAAGAAATTGACGAACTTAAAAAAGTTGCGTGTCAAAATAAAAAGTTGGA
AGATAAATCAAAAGATTATCAAGAAAAAATCAATGAAATTCAGATTTTGA
ATGCTCAATTGGATGAGAAATGTGAAGAAATCAGAAACCTGAAATTCGAG
AACCGGGAATCGAGCCATTTTAAGGCGCTGGCCATAGATAGGAGGACAGT
GATCAAGTCATTGACAGGTCAAATTGAGCAGTACAAATTGATGGAGACCC
AGTTCCATGAGACTTGCTCACTTCTTATGAAAACTCAATCAGAACTGAAT
GAGTTACAGAACCAGATTCGCAGTTCACAAATGAATAAGAATAGTGATAG
TTTTTCAGAAGTTTCATTTAATTCGTCAATATACAATCCGGAATCTCTTG
CTGGAATTGTCGAAATGGAACGAGATGAATTGAAAACGGAATTATCTCTA
TTGAAAGAAAGCTCTGTAACGAAACAAAGCTATGAAAATGCTATGGAAAA
AATCTCAGAACTCACGAGAGCATTGAATGATTCAAACGTTCAATGTTCCC
AGCTGCGATTGGAACATGAATCAATTGCAACTTTCTGCCAATCAATGAAT
AATTGTGCAAATCAATCAAGTGACCTTGACAGCTCCAAATGTCTGAATGA
CAGCATCCAAGAGATATTTCGACAAATCCAAACAACTGTTACCAACCAAA
AGGAAAATATTGAACATTTGATCACAGCCAATAGTATTTTGACAAATGAT
ATCAATCACAAAGTTGACATTGAAAAGAAACTCATTCTTGAAATCGATTC
AAAAACGAAACATTTTGAAAACATTGAGTTGGAATTGAGGGAAATAAAGG
AGTTATTGCTTCCAGATAAAACATTCGATATCAAGGACAAGGAATTCTTC
CTGGAAATGAAATACAAAATTATTTCCTATCAACAAACAACAGAAAACCT
CAAAAATCATACTTAAACAACTATCGAATCCTTAGGAACGAAATCGCTAC
CGTAAAGCAAATATTCGTAAATATAATCCAATCGCTTTCAGTCGATTTCC
AGAATTTCCAGTTTCTAATACTCAGCGATTCTCCCAGCAAACACTCTTTC
TCTAATCCTTTTATCGAATCTCTACATGGATTGTATAACACGCTGAAAGA
AAATCAAGCCAATCAAGATCAATTATATTCCGACACACAAGAATTAATAT
CATTAGAAAAGTCCCAAATTAAATCTGAGAAAAGAATTAAGGAACACGAA
CGAATGCAAGCCGAATTGACGGTAAAGCACAAAAACGAGCAGTTGGAAAT
GAAAACCGAACTATCGAAGTTGATATTTGAGTATCAAGAATGTCTAGTGA
ATTTGGAAGACATTAGCTCGAAATACCAGGAACAAACCGAGTTGAAAAAC
GAAAATGAGAATATTGAGAAGAGAATTTCGGAATTAACAATGCAAATAGA
TAAAATAAATGAAGAGTTATCTACCAGTGAATCAAAATTGCTTAGTAAAA
CCCAAGAAATTAAGCTAATCAACATGAATTATTCAAAAGAAATAGAAAAT
TTGAAATCATGGAATGCGACTTATAAATTGAAATCGGAAAAAAATGACGC
GGAAAAACACGAGAAAATAAAAAATCTCGAAACCAAAATTGACACAAAAC
AAAGAGAAATTATTGAAAAAGAAGATAAAATCAAAAAATTAGAAGAAAAG
AATAAAATTATATATGAAGAATTAAAATTAAAAGAAAATTTGGAAGAAAT
AATAAAACAAAAAGATAAGGAGATAAATATAAAA
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protein sequence of SMED30002942-orf-1

>SMED30002942-orf-1 ID=SMED30002942-orf-1|Name=SMED30002942-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=177bp
MQAELTVKHKNEQLEMKTELSKLIFEYQECLVNLEDISSKYQEQTELKNE
NENIEKRISELTMQIDKINEELSTSESKLLSKTQEIKLINMNYSKEIENL
KSWNATYKLKSEKNDAEKHEKIKNLETKIDTKQREIIEKEDKIKKLEEKN
KIIYEELKLKENLEEIIKQKDKEINIK
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protein sequence of SMED30002942-orf-2

>SMED30002942-orf-2 ID=SMED30002942-orf-2|Name=SMED30002942-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=612bp
MTMNDANIKCLLHCSENILQCDVKHVSDFRDITLFFKILNNKPIELDIEN
LLINLSNIFSFDIEDVLNLDDKNFLTDFELLKILLLCIVVYILTNNDKGG
SKKLNNLCDNIPIDLHKNACMMFAPLIVKFSDKIEITNKDLENALLLNDK
ENQTNFFSPIRDKHRPYLVNEKNISVHKQKIYRDMKASQSFEPFPLEMCT
PPETSSPLVKLVEYVQSPNCQEKIDLRKTNKNLEKNVNQLEHQLFMNSNF
ENELDKTIVVWKQKCKNLEKEVKSMQTFIDEKDDIIREKSKEIDELKKVA
CQNKKLEDKSKDYQEKINEIQILNAQLDEKCEEIRNLKFENRESSHFKAL
AIDRRTVIKSLTGQIEQYKLMETQFHETCSLLMKTQSELNELQNQIRSSQ
MNKNSDSFSEVSFNSSIYNPESLAGIVEMERDELKTELSLLKESSVTKQS
YENAMEKISELTRALNDSNVQCSQLRLEHESIATFCQSMNNCANQSSDLD
SSKCLNDSIQEIFRQIQTTVTNQKENIEHLITANSILTNDINHKVDIEKK
LILEIDSKTKHFENIELELREIKELLLPDKTFDIKDKEFFLEMKYKIISY
QQTTENLKNHT*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000419prepharyngeal region
PLANA:0000429neoblast
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 279..340
NoneNo IPR availableCOILSCoilCoilcoord: 382..402
NoneNo IPR availableCOILSCoilCoilcoord: 555..575
NoneNo IPR availableCOILSCoilCoilcoord: 451..478
NoneNo IPR availableCOILSCoilCoilcoord: 251..271
NoneNo IPR availableCOILSCoilCoilcoord: 223..243