SAM domain-containing protein

Overview
NameSAM domain-containing protein
Smed IDSMED30002927
Length (bp)644
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SAM domain-containing protein (SMED30002927) t-SNE clustered cells

Violin plots show distribution of expression levels for SAM domain-containing protein (SMED30002927) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SAM domain-containing protein (SMED30002927) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SAM domain-containing protein (SMED30002927) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002927

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30002927SMESG000041461.1 SmedASXL_012522SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30002927SMESG000041461.1 SmedASXL_012522SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30002927SMESG000041461.1 dd_Smed_v4_19710_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30002927SMESG000041461.1 Contig32731newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30002927SMESG000041461.1 Contig32731uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30002927SMESG000041461.1 Contig27143newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30002927SMESG000041461.1 Contig27143uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
zeta neoblastSMED30002927SMESG000041461.1 dd_Smed_v4_19710_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30002927SMESG000041461.1 SmWIOct06_011737GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30002927SMESG000041461.1 SMED_20461_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30002927SMESG000041461.1 SMED_20461_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Human
Match: SAMD15 (sterile alpha motif domain containing 15 [Source:HGNC Symbol;Acc:HGNC:18631])

HSP 1 Score: 88.1965 bits (217), Expect = 1.135e-19
Identity = 50/139 (35.97%), Postives = 82/139 (58.99%), Query Frame = 1 
Query:   34 DDKLIVLGESVCEEVNENWNINRDDEDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIF 450
            + +++ L + + E V+E+     +   +L+    L W  E+ A ++  +GFPQY      N I G++LI +  SNL +MGI +F+ +KAI+   +E L IE+P + R I LP R+ +G+YLE K H+G KSD  + + F
Sbjct:  510 EKEVVDLSQELKERVSEDDETQPEKGTELQFE-HLNWDPEEVAEWISQLGFPQYKECFITNFISGRKLIHVNCSNLPQMGITNFEDMKAISRHTQELLEIEEPLFKRSISLPYRDIIGLYLEQKGHTGIKSDSLTLSEF 647
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Human
Match: SAMD15 (sterile alpha motif domain containing 15 [Source:HGNC Symbol;Acc:HGNC:18631])

HSP 1 Score: 58.151 bits (139), Expect = 7.509e-11
Identity = 28/61 (45.90%), Postives = 41/61 (67.21%), Query Frame = 1 
Query:  268 MGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIF 450
            MGI +F+ +KAI+   +E L IE+P + R I LP R+ +G+YLE K H+G KSD  + + F
Sbjct:    1 MGITNFEDMKAISRHTQELLEIEEPLFKRSISLPYRDIIGLYLEQKGHTGIKSDSLTLSEF 61
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Fly
Match: CG15625 (gene:FBgn0031644 transcript:FBtr0077390)

HSP 1 Score: 68.9366 bits (167), Expect = 1.314e-13
Identity = 35/107 (32.71%), Postives = 52/107 (48.60%), Query Frame = 1 
Query:  112 DDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDK 432
            D L +P    W   D   ++   G+PQYMN    N I G++L+ ++AS L  M IK+FDHI+ I+  +R     E   +   I LP      +YL     +G   D+
Sbjct:   69 DKLPLPSIFEWDDMDIRRWINGYGYPQYMNTFRVNMITGRKLLLLDASALCAMNIKNFDHIRHISYGIRMLFHFELTKFSSSISLPDEKPNELYLLFHTQTGVNYDE 175
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Fly
Match: CG13996 (gene:FBgn0031763 transcript:FBtr0079181)

HSP 1 Score: 52.7582 bits (125), Expect = 3.328e-8
Identity = 18/59 (30.51%), Postives = 38/59 (64.41%), Query Frame = 1 
Query:  142 WSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVR 318
            WS+ D  +++++ G+P+Y      N IDG +L+ ++A  LV + +++F+HI+ +   +R
Sbjct:   87 WSINDVTDWLRNFGYPEYEQTFRENYIDGHKLLNLDAVALVALNVRNFEHIRHLGRGIR 145
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Xenopus
Match: smg8 (SMG8 nonsense mediated mRNA decay factor [Source:Xenbase;Acc:XB-GENE-965595])

HSP 1 Score: 90.8929 bits (224), Expect = 7.620e-23
Identity = 40/117 (34.19%), Postives = 68/117 (58.12%), Query Frame = 1 
Query:  109 EDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDE 459
            E + R P  L WS  +   +++  GFPQY      N I G++LI +  S L ++G+ DF H+K I+  +R+ L I +P + R + LP R+ MG++LE K+ +G  ++  +Y+ F  +
Sbjct:   13 EIEQRGPDCLRWSCREVGGWLRFKGFPQYEACFTQNYISGRKLIHVNCSTLPQIGVTDFQHMKEISRLIRDLLNITEPLWTRSVSLPHRDNMGLFLEKKSQTGLNTESLTYSQFIKQ 129
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Mouse
Match: Samd15 (sterile alpha motif domain containing 15 [Source:MGI Symbol;Acc:MGI:2685109])

HSP 1 Score: 97.0561 bits (240), Expect = 5.564e-23
Identity = 48/107 (44.86%), Postives = 66/107 (61.68%), Query Frame = 1 
Query:  136 LFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTD 456
            L WS E  A ++ D+GFPQY      N I+G++LI +  SNL +MGI DF+ +KAI+   R  L IE+P + R I LP R+  G++ E K HSG KSD  + A F +
Sbjct:  478 LTWSPERVAEWISDLGFPQYKECFTENFINGQKLIHVNCSNLPQMGITDFEDMKAISYHTRVLLGIEEPLFSRSISLPYRDNKGLFFEQKGHSGVKSDSLTLAKFVE 584
[Close]
BLAST of SAM domain-containing protein vs. UniProt/SwissProt
Match: sp|F6XZJ7|SAM15_MOUSE (Sterile alpha motif domain-containing protein 15 OS=Mus musculus OX=10090 GN=Samd15 PE=4 SV=2)

HSP 1 Score: 97.0561 bits (240), Expect = 3.892e-22
Identity = 48/107 (44.86%), Postives = 66/107 (61.68%), Query Frame = 1 
Query:  136 LFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTD 456
            L WS E  A ++ D+GFPQY      N I+G++LI +  SNL +MGI DF+ +KAI+   R  L IE+P + R I LP R+  G++ E K HSG KSD  + A F +
Sbjct:  478 LTWSPERVAEWISDLGFPQYKECFTENFINGQKLIHVNCSNLPQMGITDFEDMKAISYHTRVLLGIEEPLFSRSISLPYRDNKGLFFEQKGHSGVKSDSLTLAKFVE 584
[Close]
BLAST of SAM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q95JY5|SAM15_MACFA (Sterile alpha motif domain-containing protein 15 OS=Macaca fascicularis OX=9541 GN=SAMD15 PE=2 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 4.846e-19
Identity = 46/105 (43.81%), Postives = 65/105 (61.90%), Query Frame = 1 
Query:  136 LFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIF 450
            L W  E  A ++  +GFPQY      N I G++LI +  SNL +MGI +F+ +KAI+   RE L IE+P + R I LP R+ +G+YLE K H+G KSD  + + F
Sbjct:  536 LNWDPEKVAEWISQLGFPQYKGCFITNFISGRKLIHVNCSNLPQMGITNFEDMKAISRHTRELLEIEEPLFKRSISLPHRDIIGLYLEQKGHTGIKSDALTLSEF 640
[Close]
BLAST of SAM domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9P1V8|SAM15_HUMAN (Sterile alpha motif domain-containing protein 15 OS=Homo sapiens OX=9606 GN=SAMD15 PE=2 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 5.451e-19
Identity = 50/139 (35.97%), Postives = 82/139 (58.99%), Query Frame = 1 
Query:   34 DDKLIVLGESVCEEVNENWNINRDDEDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIF 450
            + +++ L + + E V+E+     +   +L+    L W  E+ A ++  +GFPQY      N I G++LI +  SNL +MGI +F+ +KAI+   +E L IE+P + R I LP R+ +G+YLE K H+G KSD  + + F
Sbjct:  510 EKEVVDLSQELKERVSEDDETQPEKGTELQFE-HLNWDPEEVAEWISQLGFPQYKECFITNFISGRKLIHVNCSNLPQMGITNFEDMKAISRHTQELLEIEEPLFKRSISLPYRDIIGLYLEQKGHTGIKSDSLTLSEF 647
[Close]
BLAST of SAM domain-containing protein vs. TrEMBL
Match: K1RET5 (SAM domain-containing protein OS=Crassostrea gigas OX=29159 GN=CGI_10017842 PE=4 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 2.562e-37
Identity = 65/135 (48.15%), Postives = 86/135 (63.70%), Query Frame = 1 
Query:  103 DDEDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETEGKIWQSPLTNEGMF 507
            +D  D  VP  L+WSV+  A +++ IGFP Y + I +NRIDG++LI +EAS L  +GI DF HIK I+ AVRE L IE+PD+ R I LPPR  +GMYLE K H+G+  D  +Y  F    +   WQ PL N  + 
Sbjct:    9 EDLKDDSVPPCLYWSVDKVAEWIEKIGFPNYKSCITSNRIDGRKLITLEASQLPNIGITDFKHIKTISKAVRELLFIEEPDWKRSISLPPRENLGMYLERKGHNGKNLDGLTYKSFLLNLKDSKWQPPLANHCLI 143
[Close]
BLAST of SAM domain-containing protein vs. TrEMBL
Match: A0A1S3K6N1 (sterile alpha motif domain-containing protein 15-like OS=Lingula unguis OX=7574 GN=LOC106179289 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 5.802e-35
Identity = 59/139 (42.45%), Postives = 89/139 (64.03%), Query Frame = 1 
Query:  103 DDEDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETEGKIWQSPLTNEGMFSSYA 519
            +D  D R+P    W+ E    +++++GFPQY +   +N IDGK+LI + AS L K+GI DF+HIK I+ ++R+ LTIE P++ R I LPPR  +GMYLE K+ +G K+D+ +Y  F    E   WQ PL+N  +   ++
Sbjct:   23 EDLRDERIPSCQQWTCEQVGKWIEELGFPQYKDCFVSNLIDGKKLILISASALPKIGITDFEHIKFISGSIRQLLTIEHPNWSRSISLPPRETLGMYLERKSKTGEKADELTYKRFLMNFEDYKWQPPLSNHCLILPHS 161
[Close]
BLAST of SAM domain-containing protein vs. TrEMBL
Match: A0A2T7PQW6 (SAM domain-containing protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_02744 PE=4 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 6.402e-35
Identity = 57/135 (42.22%), Postives = 90/135 (66.67%), Query Frame = 1 
Query:  103 DDEDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETEGKIWQSPLTNEGMF 507
            +D  D RVP AL+W+ E  A +++++GFP Y +    N IDG+RL+ +EAS L ++GI DF+HIK I  ++R+ L +E+PD+ R I +PPR+ +GMYLE+K++ G+  D +++  F  +     WQ PL N G+ 
Sbjct:   11 EDLKDERVPPALYWTTEQVAEWIEELGFPHYKSCFTTNFIDGRRLMRIEASALPRIGIVDFEHIKIIAKSIRDLLRVEEPDWTRSISIPPRSDLGMYLEMKSNRGKYMDTTTFEQFLQDYPNPKWQPPLANVGLI 145
[Close]
BLAST of SAM domain-containing protein vs. TrEMBL
Match: A0A2C9LJF0 (SAM domain-containing protein OS=Biomphalaria glabrata OX=6526 GN=106062900 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.095e-34
Identity = 54/135 (40.00%), Postives = 89/135 (65.93%), Query Frame = 1 
Query:  103 DDEDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETEGKIWQSPLTNEGMF 507
            +D  D RVP AL+W+ E  A++++++GFPQY   I  N I+G++L F+ AS   ++GI DF+HIK I+  +R+ + +E+P + R I +PP+N +GMYLELK+H+G+  D +++           WQ PL+N+ + 
Sbjct:   10 EDLKDKRVPSALYWTTEHVADWVEELGFPQYKACILTNLINGRKLCFLHASAFPRIGITDFEHIKIISKNIRDLIYLEEPFWNRSIAVPPKNNLGMYLELKSHTGKTIDTTTFKQMYQNNPDPKWQPPLSNQCLI 144
[Close]
BLAST of SAM domain-containing protein vs. TrEMBL
Match: A0A433T3X1 (SAM domain-containing protein OS=Elysia chlorotica OX=188477 GN=EGW08_015970 PE=4 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 3.901e-33
Identity = 55/135 (40.74%), Postives = 85/135 (62.96%), Query Frame = 1 
Query:  103 DDEDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETEGKIWQSPLTNEGMF 507
            +D  D RVP AL+W+VE  A +++++GFPQY      N IDG++L  + AS   ++GI DF+HIK I   +R+ + +E+P + R I  PP+N +GMYLELK+H+G+  D +++           WQ PL+N+ M 
Sbjct:   10 EDLKDERVPSALYWTVEHVAEWIEELGFPQYKACFLTNMIDGRKLCCIHASAFPRIGITDFEHIKIIAKNIRDLIYLEEPYWNRSIAEPPKNNLGMYLELKSHTGKVMDTTTFKQMYLNCPDPKWQPPLSNQCMI 144
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Cavefish
Match: ENSAMXT00000040150.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:46330990:46335498:-1 gene:ENSAMXG00000031813.1 transcript:ENSAMXT00000040150.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 79.337 bits (194), Expect = 5.246e-19
Identity = 34/85 (40.00%), Postives = 57/85 (67.06%), Query Frame = 1 
Query:  214 NRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETEG 468
            N I G++LI++    L ++G+ DF H+KAI+A VRE L + +P + R I  PPR+ MGM+LE+K+ +G ++D  +Y  F ++ + 
Sbjct:   26 NLITGRKLIYVNCRYLPRLGMTDFQHMKAISAHVRELLGVSEPLWSRSIADPPRDNMGMFLEIKSRTGNRADSLTYKQFLNDQKA 110
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Nematostella
Match: EDO37353 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SG04])

HSP 1 Score: 98.5969 bits (244), Expect = 1.125e-26
Identity = 46/114 (40.35%), Postives = 73/114 (64.04%), Query Frame = 1 
Query:  124 VPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETE 465
             P  + W++ED A +++ +GF QY      N ++G+RLI ++AS L +MG+ DF+HIK I+  +RE L IEDP + R I LP R  +GMYLE K+ +G ++D  ++  +  + E
Sbjct:    8 TPSCIKWTIEDVAEWIEYLGFKQYRRPFSDNLVNGRRLIQVDASTLPRMGVTDFEHIKFISKKIREALGIEDPYWNRSISLPHREVLGMYLEKKSKTGAEADILTFEEYIRQLE 121
[Close]
BLAST of SAM domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000045480.1 (sterile alpha motif domain containing 15 [Source:NCBI gene;Acc:101158569])

HSP 1 Score: 90.5077 bits (223), Expect = 6.271e-23
Identity = 41/105 (39.05%), Postives = 65/105 (61.90%), Query Frame = 1 
Query:  136 LFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIF 450
            L W     AN+++ +G+PQY      N+I+G++LIF+  SNL K+GI DF  ++ I+A VRE L I +  +   I  PPR+ M ++LE K+ +G ++D  +Y  F
Sbjct:   18 LQWDCVSVANWIESLGYPQYKACFTENQINGRKLIFVNCSNLPKLGIVDFKDMQVISARVRELLGITETPWSHSIADPPRDAMALFLERKSRTGERADSLTYQQF 122
[Close]
BLAST of SAM domain-containing protein vs. Planmine SMEST
Match: SMESG000041461.1 (SMESG000041461.1)

HSP 1 Score: 358.607 bits (919), Expect = 4.722e-128
Identity = 171/173 (98.84%), Postives = 172/173 (99.42%), Query Frame = 1 
Query:   31 MDDKLIVLGESVCEEVNENWNINRDDEDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETEGKIWQSPLTNEGMFSSYAATGCNTMYPV 549
            MDDKLIVLGESVCEEVNENWNINRDDEDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIE+PDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETEGKIWQ PLTNEGMFSSYAATGCNTMYPV
Sbjct:    1 MDDKLIVLGESVCEEVNENWNINRDDEDDLRVPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLTIEEPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETEGKIWQPPLTNEGMFSSYAATGCNTMYPV 173
[Close]
BLAST of SAM domain-containing protein vs. Planmine SMEST
Match: SMESG000044180.1 (SMESG000044180.1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.566e-11
Identity = 25/59 (42.37%), Postives = 36/59 (61.02%), Query Frame = 1 
Query:  124 VPVALFWSVEDAANFMKDIGFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKA 300
            VP  L+W+ E+ ANF+ + GF  Y +    N IDG++L  M  SNL K+GI  ++ IK 
Sbjct:   18 VPSCLYWTCEEVANFIDEKGFSNYRDCFLKNFIDGRKLFLMNQSNLPKIGISKYEDIKV 76
The following BLAST results are available for this feature:
BLAST of SAM domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
SAMD151.135e-1935.97sterile alpha motif domain containing 15 [Source:H... [more]
SAMD157.509e-1145.90sterile alpha motif domain containing 15 [Source:H... [more]
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BLAST of SAM domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CG156251.314e-1332.71gene:FBgn0031644 transcript:FBtr0077390[more]
CG139963.328e-830.51gene:FBgn0031763 transcript:FBtr0079181[more]
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BLAST of SAM domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smg87.620e-2334.19SMG8 nonsense mediated mRNA decay factor [Source:X... [more]
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BLAST of SAM domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Samd155.564e-2344.86sterile alpha motif domain containing 15 [Source:M... [more]
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BLAST of SAM domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|F6XZJ7|SAM15_MOUSE3.892e-2244.86Sterile alpha motif domain-containing protein 15 O... [more]
sp|Q95JY5|SAM15_MACFA4.846e-1943.81Sterile alpha motif domain-containing protein 15 O... [more]
sp|Q9P1V8|SAM15_HUMAN5.451e-1935.97Sterile alpha motif domain-containing protein 15 O... [more]
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BLAST of SAM domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
K1RET52.562e-3748.15SAM domain-containing protein OS=Crassostrea gigas... [more]
A0A1S3K6N15.802e-3542.45sterile alpha motif domain-containing protein 15-l... [more]
A0A2T7PQW66.402e-3542.22SAM domain-containing protein OS=Pomacea canalicul... [more]
A0A2C9LJF01.095e-3440.00SAM domain-containing protein OS=Biomphalaria glab... [more]
A0A433T3X13.901e-3340.74SAM domain-containing protein OS=Elysia chlorotica... [more]
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BLAST of SAM domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSAMXT00000040150.15.246e-1940.00pep primary_assembly:Astyanax_mexicanus-2.0:3:4633... [more]
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BLAST of SAM domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SAM domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO373531.125e-2640.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SAM domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSORLT00000045480.16.271e-2339.05sterile alpha motif domain containing 15 [Source:N... [more]
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BLAST of SAM domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000041461.14.722e-12898.84SMESG000041461.1[more]
SMESG000044180.13.566e-1142.37SMESG000044180.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002927 ID=SMED30002927|Name=SAM domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=644bp
AATTAATTGAAACTTTGTTTCAATAATAAAATGGATGATAAATTAATTGT
TTTAGGCGAAAGCGTTTGTGAAGAAGTAAATGAAAACTGGAACATAAATC
GGGATGACGAAGATGATTTAAGAGTTCCAGTTGCTTTGTTTTGGTCTGTT
GAAGACGCTGCAAATTTTATGAAAGATATTGGATTCCCTCAATATATGAA
CGCCATTTATGCAAATAGGATAGATGGTAAACGGTTAATATTCATGGAAG
CATCTAATCTAGTAAAAATGGGAATTAAAGATTTTGATCATATTAAAGCA
ATTACGGCTGCTGTAAGAGAGCGCTTAACTATCGAAGATCCTGATTATGG
AAGAAGAATTTATCTTCCTCCACGAAACAAAATGGGAATGTATCTGGAAT
TGAAAGCCCACTCGGGTAGAAAGAGTGATAAATCCAGTTATGCAATATTT
ACAGATGAGACTGAGGGTAAAATATGGCAGTCTCCATTAACGAATGAAGG
AATGTTCTCCAGTTACGCTGCGACCGGTTGTAACACAATGTACCCTGTTT
AAATATTAGGGATTTTAAAAAGGATAAATTTTAAAAATTGCTTTTTGTTT
TACAATTCAATTTATTTTATATTTCGATCTAGAATAAAAATTTT
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protein sequence of SMED30002927-orf-1

>SMED30002927-orf-1 ID=SMED30002927-orf-1|Name=SMED30002927-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=174bp
MDDKLIVLGESVCEEVNENWNINRDDEDDLRVPVALFWSVEDAANFMKDI
GFPQYMNAIYANRIDGKRLIFMEASNLVKMGIKDFDHIKAITAAVRERLT
IEDPDYGRRIYLPPRNKMGMYLELKAHSGRKSDKSSYAIFTDETEGKIWQ
SPLTNEGMFSSYAATGCNTMYPV*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000429neoblast
PLANA:0002089reproductive organ
PLANA:0002109X1 cell
PLANA:0002111X2 cell
Vocabulary: INTERPRO
TermDefinition
IPR042554SAMD15
IPR001660SAM
IPR013761SAM/pointed_sf
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0003674molecular_function
Vocabulary: biological process
TermDefinition
GO:0000027ribosomal large subunit assembly
GO:0006364rRNA processing
Vocabulary: cellular component
TermDefinition
GO:0030687preribosome, large subunit precursor
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001660Sterile alpha motif domainSMARTSM00454SAM_4coord: 35..101
e-value: 7.3E-9
score: 45.4
IPR001660Sterile alpha motif domainPFAMPF00536SAM_1coord: 38..96
e-value: 4.0E-12
score: 46.4
IPR001660Sterile alpha motif domainPROSITEPS50105SAM_DOMAINcoord: 38..101
score: 12.025
IPR013761Sterile alpha motif/pointed domain superfamilyGENE3DG3DSA:1.10.150.50coord: 36..101
e-value: 1.0E-12
score: 50.2
IPR013761Sterile alpha motif/pointed domain superfamilySUPERFAMILYSSF47769SAM/Pointed domaincoord: 37..99
IPR042554Sterile alpha motif domain-containing protein 15PANTHERPTHR46829FAMILY NOT NAMEDcoord: 26..154