SMED30002886
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30002886 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Alignments
SMED30002886 aligns in the following genomic locations:
Homology
BLAST of SMED30002886 vs. TrEMBL
Match: A0A267EXH3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig019845g1 PE=4 SV=1) HSP 1 Score: 126.331 bits (316), Expect = 2.469e-31 Identity = 88/253 (34.78%), Postives = 118/253 (46.64%), Query Frame = 2 Query: 26 MASNVELSIEPVAPLIVDPNYDIHTQRPRTPNIPYSNYSETQIKNIHQNEETFFHYL-------PVLPD------FDYNKYKKKLVDFD------------YTSDEASCRSRARHNASRPGAYFHWGKFPSPGDNICKHFHCESKESFTEIDMYRRPPTNRPGRIVMKFGRPNRDYYAPKYPSSTTWFQSDCPLNQCASFGMNKPMTFKQHNAYKKEEKDYFKSQM--SKYPNISEYIDKYTLENRTMPTMTN 703 MASNVEL D + D +T P+TPN+PYSN + Q K+I +N FFH +PD + ++Y + Y D H+ASRPGAYF WG + G+ HF ES +F +D Y RPPTN PG I K+GRP YYA + P S TWF S LN+ +P T ++ E + ++ KYP SEY KYT++ RT PT ++ Sbjct: 1 MASNVELKRTKNLEYSCDESTDPYTGLPKTPNVPYSNMTSEQEKSIKKNPHAFFHESKEEPREPGCIPDPREGGRYYIDRYADSMSQHLFRDQPTPSNPHCYIGDRLDRELPPDHSASRPGAYFSWGPNRA-GEKTLGHFDGESPVAFLPVDTYHRPPTNLPGEITFKYGRPGDGYYAQRNPQSNTWFASSHRLNEQRILQNIRPKTLAEYEQISSESRSLAATRRDEEKYPEYSEYTAKYTVQERT-PTASS 251
BLAST of SMED30002886 vs. TrEMBL
Match: A0A267FGG0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig003160g1 PE=4 SV=1) HSP 1 Score: 126.331 bits (316), Expect = 2.576e-31 Identity = 88/253 (34.78%), Postives = 118/253 (46.64%), Query Frame = 2 Query: 26 MASNVELSIEPVAPLIVDPNYDIHTQRPRTPNIPYSNYSETQIKNIHQNEETFFHYL-------PVLPD------FDYNKYKKKLVDFD------------YTSDEASCRSRARHNASRPGAYFHWGKFPSPGDNICKHFHCESKESFTEIDMYRRPPTNRPGRIVMKFGRPNRDYYAPKYPSSTTWFQSDCPLNQCASFGMNKPMTFKQHNAYKKEEKDYFKSQM--SKYPNISEYIDKYTLENRTMPTMTN 703 MASNVEL D + D +T P+TPN+PYSN + Q K+I +N FFH +PD + ++Y + Y D H+ASRPGAYF WG + G+ HF ES +F +D Y RPPTN PG I K+GRP YYA + P S TWF S LN+ +P T ++ E + ++ KYP SEY KYT++ RT PT ++ Sbjct: 1 MASNVELKRTKNLEYSCDESTDPYTGLPKTPNVPYSNMTSEQEKSIKKNPHAFFHESKEEPREPGCIPDPREGGRYYIDRYANSMSQHLFRDQPTPSNPHCYIGDRLDRELPPDHSASRPGAYFSWGPNRA-GEKTLGHFDGESPVAFLPVDTYHRPPTNLPGEITFKYGRPGDGYYAQRNPQSNTWFASSHRLNEQRILQNIRPKTLAEYEQISSESRSLAATRRDEEKYPEYSEYTAKYTVQERT-PTASS 251
BLAST of SMED30002886 vs. TrEMBL
Match: A0A1I8IGQ4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 116.701 bits (291), Expect = 1.192e-27 Identity = 83/244 (34.02%), Postives = 115/244 (47.13%), Query Frame = 2 Query: 26 MASNVELSIEPVAPLIVDPNYDIHTQRPRTPNIPYSNYSET-QIKNIHQNEETFFHYLPVLPD---FDYNKYKKKLVDFD------------YTSDEASCRSRARHNASRPGAYFHWGKFPSPGDNICKHFHCESKESFTEIDMYRRPPTNRPGRIVMKFGRPNRDYYAPKYPSSTTWFQSDCPLNQCASFGMNKPMTFKQHNAYKKEEKDYFKSQM--SKYPNISEYIDKYTLENRTMPTMTN 703 MASNVEL D + D +T P+TPN+PYSN +++ +I + Q P P+ + ++Y + Y D H+ASRPGAYF WG + G+ HF ES +F +D Y RPPTN PG I K+GRP YYA + P S TWF S LN+ +P T ++ E + ++ KYP SEY KYT++ RT PT ++ Sbjct: 1 MASNVELKRTKNLEYSCDESTDPYTGLPKTPNVPYSNMTKSARIFSRVQGGAARARLHPGSPEGGRYYIDRYADSMSQHLFRDQPTPSNPHCYIGDRLDRELPPDHSASRPGAYFSWGPNRA-GEKTLGHFDGESPVAFLPVDTYHRPPTNLPGEITFKYGRPGDGYYAQRNPQSNTWFASSHRLNEQRILQNIRPKTLAEYEQISSESRSLAATRRDEEKYPEYSEYTAKYTVQERT-PTASS 242
BLAST of SMED30002886 vs. TrEMBL
Match: A0A1I8HDV6 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 116.316 bits (290), Expect = 1.230e-27 Identity = 83/244 (34.02%), Postives = 115/244 (47.13%), Query Frame = 2 Query: 26 MASNVELSIEPVAPLIVDPNYDIHTQRPRTPNIPYSNYSET-QIKNIHQNEETFFHYLPVLPD---FDYNKYKKKLVDFD------------YTSDEASCRSRARHNASRPGAYFHWGKFPSPGDNICKHFHCESKESFTEIDMYRRPPTNRPGRIVMKFGRPNRDYYAPKYPSSTTWFQSDCPLNQCASFGMNKPMTFKQHNAYKKEEKDYFKSQM--SKYPNISEYIDKYTLENRTMPTMTN 703 MASNVEL D + D +T P+TPN+PYSN +++ +I + Q P P+ + ++Y + Y D H+ASRPGAYF WG + G+ HF ES +F +D Y RPPTN PG I K+GRP YYA + P S TWF S LN+ +P T ++ E + ++ KYP SEY KYT++ RT PT ++ Sbjct: 1 MASNVELKRTKNLEYSCDESTDPYTGLPKTPNVPYSNMTKSARIFSRVQGGAARARLHPGSPEGGRYYIDRYADSMSQHLFRDQPTPSNPHCYIGDRLDRELPPDHSASRPGAYFSWGPNRA-GEKTLGHFDGESPVAFLPVDTYHRPPTNLPGEITFKYGRPGDGYYAQRNPQSNTWFASSHRLNEQRILQNIRPKTLAEYEQISSESRSLAATRRDEEKYPEYSEYTAKYTVQERT-PTASS 242
BLAST of SMED30002886 vs. TrEMBL
Match: A0A2T7PUJ2 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_04085 PE=4 SV=1) HSP 1 Score: 112.464 bits (280), Expect = 3.474e-26 Identity = 76/236 (32.20%), Postives = 108/236 (45.76%), Query Frame = 2 Query: 71 IVDPNYDIHTQRPRTPNIPYSNYSETQIKN--IHQNEETFFHYLPVL----PDFDYNKYKKKLVDFD-----------YTSDEASCRSRARHNASRPGAYFHWGKFPSPGDNICKHFHCESKESFTEIDMYRRPPTNRPGRIVMKFGRPNRDYYAPKYPSSTTWFQSDCPLNQCASFGMNKPMTFKQHNAYKKEEKDYFKSQMSKYPNISEYIDKYTLENRTMPTMTNMERRIAHL 727 + N D+ T+RPRTP+ Y+ +E QI N I+ FF L + ++Y + + D + +HN R GA WG + G HF E + ID+Y RPPTN G +V K GRP R YY ++P S TWF S LNQ +P T K+H + EK + K+P SEY+D+YTL ++ P +T+ ER H+ Sbjct: 1 MASENIDMITKRPRTPHDQYTCMTEEQIHNNTINGRPNRFFMSQKPLVMSKKRYCMDRYATNTSQYLFREQPRSYPKCWIGDLWQQQPSEQHNTCRLGASLRWGPEKN-GSRTYSHFVGEDTHRYLNIDVYCRPPTNLVGLVVQKHGRPGRSYYDQRHPHSATWFGSSHILNQTDILQDIRPKTLKEHQMSSEAEKTKMIWRHGKWPEFSEYVDRYTLHSKDEPELTSFERNKRHI 235
BLAST of SMED30002886 vs. Planmine SMEST
Match: SMESG000017779.1 (SMESG000017779.1) HSP 1 Score: 499.975 bits (1286), Expect = 0.000e+0 Identity = 237/238 (99.58%), Postives = 237/238 (99.58%), Query Frame = 2 Query: 26 MASNVELSIEPVAPLIVDPNYDIHTQRPRTPNIPYSNYSETQIKNIHQNEETFFHYLPVLPDFDYNKYKKKLVDFDYTSDEASCRSRARHNASRPGAYFHWGKFPSPGDNICKHFHCESKESFTEIDMYRRPPTNRPGRIVMKFGRPNRDYYAPKYPSSTTWFQSDCPLNQCASFGMNKPMTFKQHNAYKKEEKDYFKSQMSKYPNISEYIDKYTLENRTMPTMTNMERRIAHLRGTL 739 MASNVELSIEPVAPLIVDPNYDIHTQRPRTPNIPYSNYSETQIKNIHQNEETFFHYLPVLPDFDYNKYKKKLVDFDYTSDEASCRSRARHNASRPGAYFHWG FPSPGDNICKHFHCESKESFTEIDMYRRPPTNRPGRIVMKFGRPNRDYYAPKYPSSTTWFQSDCPLNQCASFGMNKPMTFKQHNAYKKEEKDYFKSQMSKYPNISEYIDKYTLENRTMPTMTNMERRIAHLRGTL Sbjct: 1 MASNVELSIEPVAPLIVDPNYDIHTQRPRTPNIPYSNYSETQIKNIHQNEETFFHYLPVLPDFDYNKYKKKLVDFDYTSDEASCRSRARHNASRPGAYFHWGTFPSPGDNICKHFHCESKESFTEIDMYRRPPTNRPGRIVMKFGRPNRDYYAPKYPSSTTWFQSDCPLNQCASFGMNKPMTFKQHNAYKKEEKDYFKSQMSKYPNISEYIDKYTLENRTMPTMTNMERRIAHLRGTL 238 The following BLAST results are available for this feature:
BLAST of SMED30002886 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30002886 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30002886 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30002886 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30002886 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30002886 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30002886 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30002886 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30002886 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30002886 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30002886 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30002886 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30002886 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30002886 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30002886 ID=SMED30002886|Name=SMED30002886|organism=Schmidtea mediterranea sexual|type=transcript|length=820bpback to top protein sequence of SMED30002886-orf-1 >SMED30002886-orf-1 ID=SMED30002886-orf-1|Name=SMED30002886-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=239bp MASNVELSIEPVAPLIVDPNYDIHTQRPRTPNIPYSNYSETQIKNIHQNEback to top Annotated Terms
The following terms have been associated with this transcript:
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