Tgs-1
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30002872 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8
Homology
BLAST of Tgs-1 vs. TrEMBL
Match: A0A2Z4ELZ5 (Tgs-1 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 543.117 bits (1398), Expect = 0.000e+0 Identity = 285/308 (92.53%), Postives = 286/308 (92.86%), Query Frame = 3 Query: 483 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSET-----------------PLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKR----KSLSPLKWNKKCIVPNLNEFKKRKLAAN 1343 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSE+ PLGILSALKVSINTRT PTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKR KSLSPLKWNKKCIVPNLNEFKKRKLAAN Sbjct: 144 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSESIKLCKDMIVLVEHLFLAPLGILSALKVSINTRTAPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN 451 HSP 2 Score: 295.049 bits (754), Expect = 0.000e+0 Identity = 141/143 (98.60%), Postives = 141/143 (98.60%), Query Frame = 1 Query: 13 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE 441 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNI LTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLG DEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE Sbjct: 1 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNIPLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGFDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE 143
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000058876.1 (SMESG000058876.1) HSP 1 Score: 561.992 bits (1447), Expect = 0.000e+0 Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 3 Query: 483 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKSLSPLKWNKKCIVPNLNEFKKRKLAAN 1343 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKSLSPLKWNKKCIVPNLNEFKKRKLAAN Sbjct: 144 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKSLSPLKWNKKCIVPNLNEFKKRKLAAN 430 HSP 2 Score: 299.286 bits (765), Expect = 0.000e+0 Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 1 Query: 13 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE 441 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE Sbjct: 1 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE 143
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000058876.1 (SMESG000058876.1) HSP 1 Score: 556.214 bits (1432), Expect = 0.000e+0 Identity = 287/291 (98.63%), Postives = 287/291 (98.63%), Query Frame = 3 Query: 483 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKR----KSLSPLKWNKKCIVPNLNEFKKRKLAAN 1343 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKR KSLSPLKWNKKCIVPNLNEFKKRKLAAN Sbjct: 144 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN 434 HSP 2 Score: 299.286 bits (765), Expect = 0.000e+0 Identity = 143/143 (100.00%), Postives = 143/143 (100.00%), Query Frame = 1 Query: 13 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE 441 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE Sbjct: 1 MEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE 143
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000058876.1 (SMESG000058876.1) HSP 1 Score: 555.829 bits (1431), Expect = 0.000e+0 Identity = 287/291 (98.63%), Postives = 287/291 (98.63%), Query Frame = 3 Query: 483 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKR----KSLSPLKWNKKCIVPNLNEFKKRKLAAN 1343 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKR KSLSPLKWNKKCIVPNLNEFKKRKLAAN Sbjct: 124 DLPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNKPISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVILTAFNKINSPEQIKKPSVPMIFTPTLKNQTLKRKIIVKSLSPLKWNKKCIVPNLNEFKKRKLAAN 414 HSP 2 Score: 254.988 bits (650), Expect = 0.000e+0 Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 1 Query: 73 MKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE 441 MKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE Sbjct: 1 MKIHRQNLGVDKDNNILLTNKELVIQLLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFE 123
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000023662.1 (SMESG000023662.1) HSP 1 Score: 74.3294 bits (181), Expect = 9.903e-24 Identity = 71/230 (30.87%), Postives = 119/230 (51.74%), Query Frame = 3 Query: 486 LPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPLGILSALKVSINTRTTPTNLIIDFPEKKLKLNK----PISTPTKPNIKIFHKPTTPITKSISGVPIVSFFPNSKWNSQQESVIL 1163 LP+NF+ + K + K+++D L ++ K E ++ L +IG++++ I+ K+G+L P IS +I LK L N I K +S I AVQILLL+I +R L+ K++++F I++ + + E+L ++L S F+ N+ L + K IN +N I D P + K+ I +P I K T P KS + I +F +SK N + + +L Sbjct: 129 LPINFMNYSNMDK-EVKRVLDDLKGIKLSERKTEYLKRQKLFVIGETMKTIIQKLGVLLPAKISTEIESLKSLTCLKNNKIKKQKSHILAVQILLLNIPEQSRTLLQKIIKLFNILANHAALNRNKEQLYELLKLSLFDGENNYNKLQL---SKDFINL----SNHIFDCPSELHKIVNIYMIAIVNSNQPCELI--KGTEPSNKSTNST-IDCYFSHSKINRETNADLL 347 HSP 2 Score: 55.0694 bits (131), Expect = 9.903e-24 Identity = 33/93 (35.48%), Postives = 51/93 (54.84%), Query Frame = 1 Query: 151 LLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPRRMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHIL 429 ++ +K +P AF +++Q LN L L LD+ FL + ++ R ++N R R R RSMSPSG++ E WE EIA I+++L Sbjct: 34 VVRSKNLFPTAFKKISQELLNRLDLELDKSFLPELTAGKRSRSRIASVVNK-RIPSRT--RQRSMSPSGSLNKAEKSWENNFEIANATIEYLL 123
BLAST of Tgs-1 vs. Planmine SMEST
Match: SMESG000062778.1 (SMESG000062778.1) HSP 1 Score: 76.2554 bits (186), Expect = 6.511e-19 Identity = 52/147 (35.37%), Postives = 87/147 (59.18%), Query Frame = 3 Query: 486 LPLNFLKDQPIWKTQSKQLIDTLGKSIENKTKIEKIRESNLHIIGQSLRIILNKMGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRILINKLLEMFRIISKIRKIQMSGEELGKILCQSFFEELNSETPL 926 LP NFL D P + +SK+++D L K + E + ++ L++IG++L+ IL+K+G L P IS ++ LK L N K +S I AVQILLL+I NR L+ ++E+++ + K + ++++ E+L +L S F N+ L Sbjct: 168 LPSNFLND-PNLEKESKKILDDLKVFKTAKERKEYLEKNKLNVIGEALKNILSKIGGLVPLRISSEVELLKSLMNVRDNKACKRKSHIIAVQILLLNIPEMNRTLLKSIIELYKKLMKHKTVKINNEQLYDLLNISLFNGENNYNKL 313 HSP 2 Score: 37.3502 bits (85), Expect = 6.511e-19 Identity = 38/124 (30.65%), Postives = 63/124 (50.81%), Query Frame = 1 Query: 100 VDKDNNILLTNKELVIQ-------LLEAKEEWPMAFSRVTQNRLNELKLGLDEDFLLKQHEQRKPR-RMSPMIINHNRNCGRNSNRDRSMSPSGAIKMKESKWERRSEIAMLLIDHILMKFEGK 447 V+K I+L+N IQ LLE+K +P+ F +Q LN LKL LD+ FL++ + + R ++S +I + S + +S SG++ + WE E A ++I+ I+ E K Sbjct: 30 VNKIFCIILSNIPFSIQSEIVMKSLLESKVFYPLEFKDASQELLNRLKLQLDKSFLIESNGVARSRLKISKVISSR-----IRSRSRQRVSSSGSLNRTDKSWENNVEFANVIIEVIMNNLESK 148 The following BLAST results are available for this feature:
BLAST of Tgs-1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Tgs-1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Tgs-1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of Tgs-1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Tgs-1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Tgs-1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30002872 ID=SMED30002872|Name=Tgs-1|organism=Schmidtea mediterranea sexual|type=transcript|length=1519bpback to top protein sequence of SMED30002872-orf-1 >SMED30002872-orf-1 ID=SMED30002872-orf-1|Name=SMED30002872-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=233bp MGMLFPKVISEQILQLKFLRTRSGNAIVKDRSKIEAVQILLLHISTPNRIback to top protein sequence of SMED30002872-orf-2 >SMED30002872-orf-2 ID=SMED30002872-orf-2|Name=SMED30002872-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=151bp QINNMEQIYFNDFLSSCGASSTPIMKIHRQNLGVDKDNNILLTNKELVIQback to top Annotated Terms
The following terms have been associated with this transcript:
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