Transcription factor AP-4

Overview
NameTranscription factor AP-4
Smed IDSMED30002761
Length (bp)1304
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Transcription factor AP-4 (SMED30002761) t-SNE clustered cells

Violin plots show distribution of expression levels for Transcription factor AP-4 (SMED30002761) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Transcription factor AP-4 (SMED30002761) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Transcription factor AP-4 (SMED30002761) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002761

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30002761h1SMcG0016956 DN305431smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
central nervous systemSMED30002761h1SMcG0016956 DN305431smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
progenitor cellSMED30002761h1SMcG0016956 DN305431smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
stem cellSMED30002761h1SMcG0016956 DN305431smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30002761h1SMcG0016956 DN305431smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Human
Match: TFAP4 (transcription factor AP-4 [Source:HGNC Symbol;Acc:HGNC:11745])

HSP 1 Score: 52.7582 bits (125), Expect = 6.486e-7
Identity = 42/73 (57.53%), Postives = 54/73 (73.97%), Query Frame = 3 
Query:  321 NDDDIKIXXXXXXXXXXXXMQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIVKL 539
             D + +IRRE+AN+NERRRMQSINAGF+SL+ L+P  T+ EK+SKA+ILQ TA+YI        SLE E  +L
Sbjct:   42 RDQERRIRREIANSNERRRMQSINAGFQSLKTLIPH-TDGEKLSKAAILQQTAEYIF-------SLEQEKTRL 106
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Human
Match: TFAP4 (transcription factor AP-4 [Source:HGNC Symbol;Acc:HGNC:11745])

HSP 1 Score: 48.1358 bits (113), Expect = 1.867e-6
Identity = 28/59 (47.46%), Postives = 40/59 (67.80%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIVKLSNSLE 554
            MQSINAGF+SL+ L+P  T+ EK+SKA+ILQ TA+YI  L  +   L  +  +L   ++
Sbjct:    1 MQSINAGFQSLKTLIPH-TDGEKLSKAAILQQTAEYIFSLEQEKTRLLQQNTQLKRFIQ 58
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Fly
Match: crp (gene:FBgn0001994 transcript:FBtr0343814)

HSP 1 Score: 53.5286 bits (127), Expect = 2.579e-7
Identity = 27/40 (67.50%), Postives = 32/40 (80.00%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDL 497
            MQSINAGF+SLR LLP   E EK+SKA+ILQ T +YI +L
Sbjct:  109 MQSINAGFQSLRSLLPR-HEGEKLSKAAILQQTFQYIVEL 147
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Fly
Match: crp (gene:FBgn0001994 transcript:FBtr0080854)

HSP 1 Score: 53.5286 bits (127), Expect = 2.579e-7
Identity = 27/40 (67.50%), Postives = 32/40 (80.00%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDL 497
            MQSINAGF+SLR LLP   E EK+SKA+ILQ T +YI +L
Sbjct:  109 MQSINAGFQSLRSLLPR-HEGEKLSKAAILQQTFQYIVEL 147
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Zebrafish
Match: tfap4 (transcription factor AP-4 (activating enhancer binding protein 4) [Source:ZFIN;Acc:ZDB-GENE-090810-1])

HSP 1 Score: 51.2174 bits (121), Expect = 1.257e-6
Identity = 41/73 (56.16%), Postives = 54/73 (73.97%), Query Frame = 3 
Query:  321 NDDDIKIXXXXXXXXXXXXMQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIVKL 539
             D + +IRRE+AN+NERRRMQSINAGF+SL+ L+P  ++ EK+SKA+ILQ TA+YI        SLE E  +L
Sbjct:   42 RDQERRIRREIANSNERRRMQSINAGFQSLKTLIPH-SDGEKLSKAAILQQTAEYIF-------SLEQEKTRL 106
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Xenopus
Match: rbm28 (RNA binding motif protein 28 [Source:Xenbase;Acc:XB-GENE-919947])

HSP 1 Score: 52.7582 bits (125), Expect = 4.955e-7
Identity = 42/73 (57.53%), Postives = 54/73 (73.97%), Query Frame = 3 
Query:  321 NDDDIKIXXXXXXXXXXXXMQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIVKL 539
             D + +IRRE+AN+NERRRMQSINAGF+SL+ L+P  T+ EK+SKA+ILQ TA+YI        SLE E  +L
Sbjct:   42 RDQERRIRREIANSNERRRMQSINAGFQSLKTLIPH-TDGEKLSKAAILQQTAEYIF-------SLEQEKTRL 106
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Mouse
Match: Tfap4 (transcription factor AP4 [Source:MGI Symbol;Acc:MGI:103239])

HSP 1 Score: 52.373 bits (124), Expect = 2.106e-7
Identity = 42/73 (57.53%), Postives = 54/73 (73.97%), Query Frame = 3 
Query:  321 NDDDIKIXXXXXXXXXXXXMQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIVKL 539
             D + +IRRE+AN+NERRRMQSINAGF+SL+ L+P  T+ EK+SKA+ILQ TA+YI        SLE E  +L
Sbjct:   22 RDQERRIRREIANSNERRRMQSINAGFQSLKTLIPH-TDGEKLSKAAILQQTAEYIF-------SLEQEKTRL 86
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Mouse
Match: Tfap4 (transcription factor AP4 [Source:MGI Symbol;Acc:MGI:103239])

HSP 1 Score: 52.373 bits (124), Expect = 2.165e-7
Identity = 30/54 (55.56%), Postives = 38/54 (70.37%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIVKL 539
            MQSINAGF+SL+ L+P  T+ EK+SKA+ILQ TA+YI        SLE E  +L
Sbjct:    1 MQSINAGFQSLKTLIPH-TDGEKLSKAAILQQTAEYIF-------SLEQEKTRL 46
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Mouse
Match: Tfap4 (transcription factor AP4 [Source:MGI Symbol;Acc:MGI:103239])

HSP 1 Score: 52.7582 bits (125), Expect = 4.183e-7
Identity = 42/73 (57.53%), Postives = 54/73 (73.97%), Query Frame = 3 
Query:  321 NDDDIKIXXXXXXXXXXXXMQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIVKL 539
             D + +IRRE+AN+NERRRMQSINAGF+SL+ L+P  T+ EK+SKA+ILQ TA+YI        SLE E  +L
Sbjct:   42 RDQERRIRREIANSNERRRMQSINAGFQSLKTLIPH-TDGEKLSKAAILQQTAEYIF-------SLEQEKTRL 106
[Close]
BLAST of Transcription factor AP-4 vs. UniProt/SwissProt
Match: sp|Q01664|TFAP4_HUMAN (Transcription factor AP-4 OS=Homo sapiens OX=9606 GN=TFAP4 PE=1 SV=2)

HSP 1 Score: 52.7582 bits (125), Expect = 3.115e-6
Identity = 42/73 (57.53%), Postives = 54/73 (73.97%), Query Frame = 3 
Query:  321 NDDDIKIXXXXXXXXXXXXMQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIVKL 539
             D + +IRRE+AN+NERRRMQSINAGF+SL+ L+P  T+ EK+SKA+ILQ TA+YI        SLE E  +L
Sbjct:   42 RDQERRIRREIANSNERRRMQSINAGFQSLKTLIPH-TDGEKLSKAAILQQTAEYIF-------SLEQEKTRL 106
[Close]
BLAST of Transcription factor AP-4 vs. TrEMBL
Match: A0A0R3SX86 (BHLH domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=HDID_LOCUS10340 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.057e-8
Identity = 34/51 (66.67%), Postives = 39/51 (76.47%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEI 530
            MQSINAGFE LR LLP   + EK+SKASILQ TAK IT L  KI++LE E+
Sbjct:   58 MQSINAGFEHLRGLLPPTQDGEKLSKASILQQTAKLITSLHAKISALEQEL 108
[Close]
BLAST of Transcription factor AP-4 vs. TrEMBL
Match: A0A564XXH8 (BHLH domain-containing protein OS=Hymenolepis diminuta OX=6216 GN=WMSIL1_LOCUS865 PE=4 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 1.301e-8
Identity = 34/51 (66.67%), Postives = 39/51 (76.47%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEI 530
            MQSINAGFE LR LLP   + EK+SKASILQ TAK IT L  KI++LE E+
Sbjct:   58 MQSINAGFEHLRGLLPPTQDGEKLSKASILQQTAKLITSLHAKISALEQEL 108
[Close]
BLAST of Transcription factor AP-4 vs. TrEMBL
Match: A0A0R3TYX8 (BHLH domain-containing protein OS=Rodentolepis nana OX=102285 GN=HNAJ_LOCUS13051 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.605e-8
Identity = 33/51 (64.71%), Postives = 41/51 (80.39%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEI 530
            MQSINAGFE+LR LLP   + EK+SKASILQ TAK IT+L  +I++LE E+
Sbjct:   58 MQSINAGFENLRGLLPPTQDGEKLSKASILQQTAKLITNLHGRISALEQEL 108
[Close]
BLAST of Transcription factor AP-4 vs. TrEMBL
Match: A0A0R3UIK6 (BHLH domain-containing protein OS=Mesocestoides corti OX=53468 GN=MCOS_LOCUS7267 PE=4 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 1.710e-8
Identity = 31/52 (59.62%), Postives = 42/52 (80.77%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIV 533
            MQSINAGF++LR LLP   + EK+SKA+ILQ TAK+I  L MK+++LE+E+ 
Sbjct:   59 MQSINAGFDNLRVLLPPAQDGEKLSKATILQQTAKFINTLLMKVSALEHEVA 110
[Close]
BLAST of Transcription factor AP-4 vs. TrEMBL
Match: A0A183LCD8 (BHLH domain-containing protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS1463 PE=4 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 3.831e-8
Identity = 32/52 (61.54%), Postives = 42/52 (80.77%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIV 533
            MQSINAGF+SLR LLP  ++ EK+SKA+ILQ TAKYI  L M+++ LE+E+ 
Sbjct:    1 MQSINAGFDSLRILLPSISDGEKMSKATILQLTAKYINTLLMRVSLLESEVA 52
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Cavefish
Match: tfap4 (transcription factor AP-4 [Source:NCBI gene;Acc:103031420])

HSP 1 Score: 51.2174 bits (121), Expect = 9.999e-7
Identity = 41/73 (56.16%), Postives = 54/73 (73.97%), Query Frame = 3 
Query:  321 NDDDIKIXXXXXXXXXXXXMQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIVKL 539
             D + +IRRE+AN+NERRRMQSINAGF+SL+ L+P  ++ EK+SKA+ILQ TA+YI        SLE E  +L
Sbjct:   42 RDQERRIRREIANSNERRRMQSINAGFQSLKTLIPH-SDGEKLSKAAILQQTAEYIF-------SLEQEKTRL 106
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Nematostella
Match: EDO39015 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBF3])

HSP 1 Score: 50.8322 bits (120), Expect = 6.776e-8
Identity = 24/37 (64.86%), Postives = 32/37 (86.49%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYI 488
            MQSIN+GF++LR L+P  TE EK+SKA+ILQ T++YI
Sbjct:   26 MQSINSGFQALRMLIP-NTEGEKLSKAAILQQTSEYI 61
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Medaka
Match: TFAP4 (transcription factor AP-4 [Source:NCBI gene;Acc:101157191])

HSP 1 Score: 53.1434 bits (126), Expect = 2.483e-7
Identity = 38/56 (67.86%), Postives = 47/56 (83.93%), Query Frame = 3 
Query:  321 NDDDIKIXXXXXXXXXXXXMQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYI 488
             D + +IRRE+AN+NERRRMQSINAGF+SL+ LLP  T+ EK+SKA+ILQ TA YI
Sbjct:   42 QDQERRIRREIANSNERRRMQSINAGFQSLKTLLPH-TDGEKLSKAAILQQTADYI 96
[Close]
BLAST of Transcription factor AP-4 vs. Ensembl Medaka
Match: tfap4 (transcription factor AP-4 [Source:NCBI gene;Acc:101175405])

HSP 1 Score: 50.8322 bits (120), Expect = 1.220e-6
Identity = 36/56 (64.29%), Postives = 48/56 (85.71%), Query Frame = 3 
Query:  321 NDDDIKIXXXXXXXXXXXXMQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYI 488
             D + +IRRE+AN+NERRRMQSINAGF+SL+ L+P  ++ EK+SKA+ILQ TA+YI
Sbjct:   42 RDQERRIRREIANSNERRRMQSINAGFQSLKTLIPH-SDGEKLSKAAILQQTAEYI 96
[Close]
BLAST of Transcription factor AP-4 vs. Planmine SMEST
Match: SMESG000062271.1 (SMESG000062271.1)

HSP 1 Score: 311.612 bits (797), Expect = 3.867e-105
Identity = 227/243 (93.42%), Postives = 228/243 (93.83%), Query Frame = 3 
Query:  501 MKINSLENEIVKLSNSLENDIAKISNSEKTFINKSLPKESVEKTLKSEIATEETHCXXXXXXXXXXXXXXXXXXXXXXXXXXXINFNSEEEIGVRINSFSTVNESIGFSXXXXXXXXXXXAAVEQVERLEAMQSISLKDNLNYYKTXXXXXXXXXXXXXXXXGYEKTSFFVMKPIISPFAEKIIQDVVNIKNPYKNLTTSVIGSNSFILPISCPTYIPESNKSNDYLQNSEISNNYMRINNVI 1229
            MKINSLENEIVKLSNS+ENDIAKISNSEKTFINKSLP ESVEKTLKSEIATEETHCNNSNVDSNSTNDLSSSLSSTTDYSSTLINFNSEEEIG              FSQQQQNNLEQLLAAVEQVERLEAMQSISLKDNLNYYKTKKSKLESDSNSSSLSLGYEKTSFFVMKPIISPFAEKIIQDVVNIKNPYKNLTTSVIGSNSFILPISCPTYIPESNKSNDYLQNSEISNNYMRINNVI
Sbjct:    1 MKINSLENEIVKLSNSIENDIAKISNSEKTFINKSLPTESVEKTLKSEIATEETHCNNSNVDSNSTNDLSSSLSSTTDYSSTLINFNSEEEIG--------------FSQQQQNNLEQLLAAVEQVERLEAMQSISLKDNLNYYKTKKSKLESDSNSSSLSLGYEKTSFFVMKPIISPFAEKIIQDVVNIKNPYKNLTTSVIGSNSFILPISCPTYIPESNKSNDYLQNSEISNNYMRINNVI 229
[Close]
BLAST of Transcription factor AP-4 vs. Planmine SMEST
Match: SMESG000062264.1 (SMESG000062264.1)

HSP 1 Score: 102.449 bits (254), Expect = 1.406e-26
Identity = 78/79 (98.73%), Postives = 78/79 (98.73%), Query Frame = 3 
Query:  222 MSTYSDKLERHYILGDLENXXXXXXXXXXXXXXNDDDIKIXXXXXXXXXXXXMQSINAGFESLRKLLPFGTEKEKISKA 458
            MSTYSDKLERHYILGDLENKLKRKSSRTLKLLKNDDDIKIRREVANNNERRRMQSINAGFESLRKLLPFGTEKEKISK 
Sbjct:    1 MSTYSDKLERHYILGDLENKLKRKSSRTLKLLKNDDDIKIRREVANNNERRRMQSINAGFESLRKLLPFGTEKEKISKV 79
[Close]
BLAST of Transcription factor AP-4 vs. Planmine SMEST
Match: SMESG000081049.1 (SMESG000081049.1)

HSP 1 Score: 65.855 bits (159), Expect = 1.779e-11
Identity = 32/52 (61.54%), Postives = 40/52 (76.92%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIV 533
            MQSIN GFE+L++LLP   E EK+SKASILQ TAKYI  L +++ SLE E+ 
Sbjct:   48 MQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVA 99
[Close]
BLAST of Transcription factor AP-4 vs. Planmine SMEST
Match: SMESG000081049.1 (SMESG000081049.1)

HSP 1 Score: 65.855 bits (159), Expect = 1.984e-11
Identity = 32/52 (61.54%), Postives = 40/52 (76.92%), Query Frame = 3 
Query:  378 MQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLENEIV 533
            MQSIN GFE+L++LLP   E EK+SKASILQ TAKYI  L +++ SLE E+ 
Sbjct:   55 MQSINMGFETLKQLLPMTMEGEKLSKASILQFTAKYIQSLIVRLTSLEKEVA 106
The following BLAST results are available for this feature:
BLAST of Transcription factor AP-4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TFAP46.486e-757.53transcription factor AP-4 [Source:HGNC Symbol;Acc:... [more]
TFAP41.867e-647.46transcription factor AP-4 [Source:HGNC Symbol;Acc:... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor AP-4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
crp2.579e-767.50gene:FBgn0001994 transcript:FBtr0343814[more]
crp2.579e-767.50gene:FBgn0001994 transcript:FBtr0080854[more]
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BLAST of Transcription factor AP-4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tfap41.257e-656.16transcription factor AP-4 (activating enhancer bin... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
rbm284.955e-757.53RNA binding motif protein 28 [Source:Xenbase;Acc:X... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
Tfap42.106e-757.53transcription factor AP4 [Source:MGI Symbol;Acc:MG... [more]
Tfap42.165e-755.56transcription factor AP4 [Source:MGI Symbol;Acc:MG... [more]
Tfap44.183e-757.53transcription factor AP4 [Source:MGI Symbol;Acc:MG... [more]
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BLAST of Transcription factor AP-4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q01664|TFAP4_HUMAN3.115e-657.53Transcription factor AP-4 OS=Homo sapiens OX=9606 ... [more]
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BLAST of Transcription factor AP-4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0R3SX861.057e-866.67BHLH domain-containing protein OS=Hymenolepis dimi... [more]
A0A564XXH81.301e-866.67BHLH domain-containing protein OS=Hymenolepis dimi... [more]
A0A0R3TYX81.605e-864.71BHLH domain-containing protein OS=Rodentolepis nan... [more]
A0A0R3UIK61.710e-859.62BHLH domain-containing protein OS=Mesocestoides co... [more]
A0A183LCD83.831e-861.54BHLH domain-containing protein OS=Schistosoma marg... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
tfap49.999e-756.16transcription factor AP-4 [Source:NCBI gene;Acc:10... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor AP-4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Transcription factor AP-4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO390156.776e-864.86Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Transcription factor AP-4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
TFAP42.483e-767.86transcription factor AP-4 [Source:NCBI gene;Acc:10... [more]
tfap41.220e-664.29transcription factor AP-4 [Source:NCBI gene;Acc:10... [more]
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BLAST of Transcription factor AP-4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000062271.13.867e-10593.42SMESG000062271.1[more]
SMESG000062264.11.406e-2698.73SMESG000062264.1[more]
SMESG000081049.11.779e-1161.54SMESG000081049.1[more]
SMESG000081049.11.984e-1161.54SMESG000081049.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002761 ID=SMED30002761|Name=Transcription factor AP-4|organism=Schmidtea mediterranea sexual|type=transcript|length=1304bp
GTAATTCTCAGATGTTAAGAATAAGTTATTTTTCTAATATATCACTTTAA
GTAAAAAGTATGAAAATTTTTATGGAAATTGACATGGTGTCATTTTAATT
ATTAAAAGTAAATCCGGTGCCAAAATATTCATCCATTTAGTGATTCTCAT
ATGTTAATTTATTTTGCGTCCTAGTTTGCTTAATCGATTTAACCAATAAA
TATACTTATAAATTACTTATTATGTCAACATATTCAGATAAATTGGAAAG
GCATTACATATTGGGTGATTTGGAGAATAAATTAAAACGAAAATCAAGTC
GTACTTTAAAACTTCTGAAAAATGATGATGATATAAAGATCAGAAGAGAA
GTGGCTAATAATAACGAACGCCGCAGAATGCAAAGTATTAATGCTGGTTT
TGAGAGTTTAAGAAAATTACTTCCATTTGGTACTGAAAAAGAAAAAATTA
GCAAAGCTAGCATTCTACAATCTACAGCAAAGTATATAACTGATTTAAGG
ATGAAAATTAATTCTTTAGAAAATGAGATAGTTAAATTATCGAATTCTTT
AGAAAATGATATTGCAAAAATATCAAATTCCGAAAAAACTTTTATTAATA
AATCTTTGCCAAAAGAATCTGTCGAGAAAACTTTAAAATCTGAAATCGCT
ACAGAAGAGACACATTGTAATAACAGCAATGTAGATTCAAATTCAACAAA
TGATTTAAGTTCTTCTTTGTCTTCAACGACAGATTACTCTTCTACACTTA
TTAACTTCAATTCTGAAGAAGAAATAGGTGTTCGAATTAACTCATTTTCA
ACAGTTAATGAATCAATAGGGTTTTCTCAGCAACAGCAGAATAATTTAGA
ACAGCTATTAGCTGCTGTGGAACAAGTTGAACGCCTTGAAGCTATGCAAT
CTATATCATTGAAAGACAACTTAAATTATTACAAAACAAAAAAGTCAAAA
TTAGAATCAGATTCAAATTCATCTTCTCTTTCACTTGGATATGAAAAAAC
AAGCTTTTTTGTCATGAAACCAATAATAAGCCCATTTGCGGAAAAAATCA
TACAGGATGTAGTCAACATAAAAAATCCTTATAAAAATTTAACAACATCA
GTAATTGGTTCGAATTCATTTATTTTACCTATAAGTTGTCCTACGTATAT
TCCCGAGTCTAATAAGTCCAATGATTATTTGCAAAATTCAGAAATTTCTA
ACAATTATATGCGTATAAATAATGTAATTTAAGCCAAATTATTTCAAAAA
AAATTAAAATTTTCTTTTTAAAAGTGATTTTGTGACTTGTATCTATTGTA
ATTA
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protein sequence of SMED30002761-orf-1

>SMED30002761-orf-1 ID=SMED30002761-orf-1|Name=SMED30002761-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=337bp
MSTYSDKLERHYILGDLENKLKRKSSRTLKLLKNDDDIKIRREVANNNER
RRMQSINAGFESLRKLLPFGTEKEKISKASILQSTAKYITDLRMKINSLE
NEIVKLSNSLENDIAKISNSEKTFINKSLPKESVEKTLKSEIATEETHCN
NSNVDSNSTNDLSSSLSSTTDYSSTLINFNSEEEIGVRINSFSTVNESIG
FSQQQQNNLEQLLAAVEQVERLEAMQSISLKDNLNYYKTKKSKLESDSNS
SSLSLGYEKTSFFVMKPIISPFAEKIIQDVVNIKNPYKNLTTSVIGSNSF
ILPISCPTYIPESNKSNDYLQNSEISNNYMRINNVI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000103central nervous system
PLANA:0000120progenitor cell
PLANA:0000121stem cell
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR036638HLH_DNA-bd_sf
IPR011598bHLH_dom
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 82..116
NoneNo IPR availableCOILSCoilCoilcoord: 202..222
NoneNo IPR availablePANTHERPTHR15741:SF27TRANSCRIPTION FACTOR AP-4coord: 33..93
NoneNo IPR availablePANTHERPTHR15741BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTORcoord: 33..93
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 46..98
e-value: 3.6E-13
score: 59.8
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 41..92
e-value: 1.3E-15
score: 57.1
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 40..92
score: 15.516
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainCDDcd00083HLHcoord: 41..93
e-value: 1.95227E-13
score: 62.2315
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3DG3DSA:4.10.280.10coord: 38..121
e-value: 5.3E-17
score: 63.6
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILYSSF47459HLH, helix-loop-helix DNA-binding domaincoord: 41..109