DNA topoisomerase I

Overview
NameDNA topoisomerase I
Smed IDSMED30002736
Length (bp)2519
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA topoisomerase I (SMED30002736) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA topoisomerase I (SMED30002736) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA topoisomerase I (SMED30002736) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA topoisomerase I (SMED30002736) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002736

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X2 cellSMED30002736h1SMcG0007637 SmedASXL_012596SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30002736h1SMcG0007637 Contig37153uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002736h1SMcG0007637 Contig37153newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
pharynxSMED30002736h1SMcG0007637 dd_Smed_v4_2421_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30002736h1SMcG0007637 dd_Smed_v4_2421_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30002736h1SMcG0007637 dd_Smed_v4_2421_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30002736h1SMcG0007637 dd_Smed_v4_2421_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30002736h1SMcG0007637 dd_Smed_v4_2421_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30002736h1SMcG0007637 dd_Smed_v4_2421_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002736h1SMcG0007637 dd_Smed_v4_2421_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Human
Match: TOP1 (DNA topoisomerase I [Source:HGNC Symbol;Acc:HGNC:11986])

HSP 1 Score: 684.485 bits (1765), Expect = 0.000e+0
Identity = 342/552 (61.96%), Postives = 427/552 (77.36%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLP +VKFYYDGK MKL P+ EE+ATF+AKM DH+YT  ++F KNF +DW   M+ +EK++I +LSKCDF  M  YFK  +E +K + KEEK K K +NE+++ +YG+C+MD++K++I NFKIEPPGLFRGRGNHPKMG +KRRI+PED+IINCSK++K+P PP G KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +DKIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    EY+V+FDFLGKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNT  LN++L++LM GLTAKVFRTYNASITL+++L       +N+  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK+  ++ +   K  KA+    KD+  K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRI++AWC K  VP+ KIYNKTQ +KF WA+DM  E+Y+F
Sbjct:  214 GIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF 765
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Human
Match: TOP1MT (DNA topoisomerase I mitochondrial [Source:HGNC Symbol;Acc:HGNC:29787])

HSP 1 Score: 625.935 bits (1613), Expect = 0.000e+0
Identity = 317/552 (57.43%), Postives = 409/552 (74.09%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+ LEH GP F   YEPLP  V+F+Y+G+ ++L    EE+ATFY +M DH+YT  +VF KNF  DW   M+ +E+ VIK L KCDF  +  YF   +  +K L +EEK+K K + E++  ++GYC++D +++KIGNFKIEPPGLFRGRG+HPKMG +KRRI PEDV+INCS++SKIP+PPAG +WKEVR D TVTWLA W E+VQN+ KY+MLN  S+LKGE  W+K+ETAR+L   +D+IR  Y  D+  REM+ RQRAVALYFIDKLALRAGNEK + E ADTVGCCSLRVEH+ L  ++   +++V+FDFLGKD IRY N V V K VYKNL  F E K   DDLFDRL T++LN++L+ELM GLTAKVFRTYNASITL+ +L       D+++ K+L+YN ANR VAILCNHQRA P TFEKSM+NL  KI  KK+  +E +   ++ +AE+    D   +   EK ++ LEK +E L ++  Q TD+E+NK VALGTSKLNYLDPRISIAWC +  VP+ KIY+KTQ ++F WA+ M  E+++F
Sbjct:   50 GVKWRQLEHKGPYFAPPYEPLPDGVRFFYEGRPVRLSVAAEEVATFYGRMLDHEYTTKEVFRKNFFNDWRKEMAVEEREVIKSLDKCDFTEIHRYFVDKAAARKVLSREEKQKLKEEAEKLQQEFGYCILDGHQEKIGNFKIEPPGLFRGRGDHPKMGMLKRRITPEDVVINCSRDSKIPEPPAGHQWKEVRSDNTVTWLAAWTESVQNSIKYIMLNPCSKLKGETAWQKFETARRLRGFVDEIRSQYRADWKSREMKTRQRAVALYFIDKLALRAGNEKEDGEAADTVGCCSLRVEHVQLHPEADGCQHVVEFDFLGKDCIRYYNRVPVEKPVYKNLQLFMENKDPRDDLFDRLTTTSLNKHLQELMDGLTAKVFRTYNASITLQEQLRALTRAEDSIAAKILSYNRANRVVAILCNHQRATPSTFEKSMQNLQTKIQAKKEQVAEARAELRRARAEHKAQGDGKSRSVLEKKRRLLEKLQEQLAQLSVQATDKEENKQVALGTSKLNYLDPRISIAWCKRFRVPVEKIYSKTQRERFAWALAMAGEDFEF 601
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Human
Match: TOP1MT (DNA topoisomerase I mitochondrial [Source:HGNC Symbol;Acc:HGNC:29787])

HSP 1 Score: 568.155 bits (1463), Expect = 0.000e+0
Identity = 292/503 (58.05%), Postives = 375/503 (74.55%), Query Frame = 2 
Query:  497 MFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            M DH+YT  +VF KNF  DW   M+ +E+ VIK L KCDF  +  YF   +  +K L +EEK+K K + E++  ++GYC++D +++KIGNFKIEPPGLFRGRG+HPKMG +KRRI PEDV+INCS++SKIP+PPAG +WKEVR D TVTWLA W E+VQN+ KY+MLN  S+LKGE  W+K+ETAR+L   +D+IR  Y  D+  REM+ RQRAVALYFIDKLALRAGNEK + E ADTVGCCSLRVEH+ L  ++   +++V+FDFLGKD IRY N V V K VYKNL  F E K   DDLFDRL T++LN++L+ELM GLTAKVFRTYNASITL+ +L       D+++ K+L+YN ANR VAILCNHQRA P TFEKSM+NL  KI  KK+  +E +   ++ +AE+    D   +   EK ++ LEK +E L ++  Q TD+E+NK VALGTSKLNYLDPRISIAWC +  VP+ KIY+KTQ ++F WA+ M  E+++F
Sbjct:    1 MLDHEYTTKEVFRKNFFNDWRKEMAVEEREVIKSLDKCDFTEIHRYFVDKAAARKVLSREEKQKLKEEAEKLQQEFGYCILDGHQEKIGNFKIEPPGLFRGRGDHPKMGMLKRRITPEDVVINCSRDSKIPEPPAGHQWKEVRSDNTVTWLAAWTESVQNSIKYIMLNPCSKLKGETAWQKFETARRLRGFVDEIRSQYRADWKSREMKTRQRAVALYFIDKLALRAGNEKEDGEAADTVGCCSLRVEHVQLHPEADGCQHVVEFDFLGKDCIRYYNRVPVEKPVYKNLQLFMENKDPRDDLFDRLTTTSLNKHLQELMDGLTAKVFRTYNASITLQEQLRALTRAEDSIAAKILSYNRANRVVAILCNHQRATPSTFEKSMQNLQTKIQAKKEQVAEARAELRRARAEHKAQGDGKSRSVLEKKRRLLEKLQEQLAQLSVQATDKEENKQVALGTSKLNYLDPRISIAWCKRFRVPVEKIYSKTQRERFAWALAMAGEDFEF 503
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Human
Match: TOP1MT (DNA topoisomerase I mitochondrial [Source:HGNC Symbol;Acc:HGNC:29787])

HSP 1 Score: 568.155 bits (1463), Expect = 0.000e+0
Identity = 292/503 (58.05%), Postives = 375/503 (74.55%), Query Frame = 2 
Query:  497 MFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            M DH+YT  +VF KNF  DW   M+ +E+ VIK L KCDF  +  YF   +  +K L +EEK+K K + E++  ++GYC++D +++KIGNFKIEPPGLFRGRG+HPKMG +KRRI PEDV+INCS++SKIP+PPAG +WKEVR D TVTWLA W E+VQN+ KY+MLN  S+LKGE  W+K+ETAR+L   +D+IR  Y  D+  REM+ RQRAVALYFIDKLALRAGNEK + E ADTVGCCSLRVEH+ L  ++   +++V+FDFLGKD IRY N V V K VYKNL  F E K   DDLFDRL T++LN++L+ELM GLTAKVFRTYNASITL+ +L       D+++ K+L+YN ANR VAILCNHQRA P TFEKSM+NL  KI  KK+  +E +   ++ +AE+    D   +   EK ++ LEK +E L ++  Q TD+E+NK VALGTSKLNYLDPRISIAWC +  VP+ KIY+KTQ ++F WA+ M  E+++F
Sbjct:    1 MLDHEYTTKEVFRKNFFNDWRKEMAVEEREVIKSLDKCDFTEIHRYFVDKAAARKVLSREEKQKLKEEAEKLQQEFGYCILDGHQEKIGNFKIEPPGLFRGRGDHPKMGMLKRRITPEDVVINCSRDSKIPEPPAGHQWKEVRSDNTVTWLAAWTESVQNSIKYIMLNPCSKLKGETAWQKFETARRLRGFVDEIRSQYRADWKSREMKTRQRAVALYFIDKLALRAGNEKEDGEAADTVGCCSLRVEHVQLHPEADGCQHVVEFDFLGKDCIRYYNRVPVEKPVYKNLQLFMENKDPRDDLFDRLTTTSLNKHLQELMDGLTAKVFRTYNASITLQEQLRALTRAEDSIAAKILSYNRANRVVAILCNHQRATPSTFEKSMQNLQTKIQAKKEQVAEARAELRRARAEHKAQGDGKSRSVLEKKRRLLEKLQEQLAQLSVQATDKEENKQVALGTSKLNYLDPRISIAWCKRFRVPVEKIYSKTQRERFAWALAMAGEDFEF 503
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Human
Match: TOP1MT (DNA topoisomerase I mitochondrial [Source:HGNC Symbol;Acc:HGNC:29787])

HSP 1 Score: 568.155 bits (1463), Expect = 0.000e+0
Identity = 292/503 (58.05%), Postives = 375/503 (74.55%), Query Frame = 2 
Query:  497 MFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            M DH+YT  +VF KNF  DW   M+ +E+ VIK L KCDF  +  YF   +  +K L +EEK+K K + E++  ++GYC++D +++KIGNFKIEPPGLFRGRG+HPKMG +KRRI PEDV+INCS++SKIP+PPAG +WKEVR D TVTWLA W E+VQN+ KY+MLN  S+LKGE  W+K+ETAR+L   +D+IR  Y  D+  REM+ RQRAVALYFIDKLALRAGNEK + E ADTVGCCSLRVEH+ L  ++   +++V+FDFLGKD IRY N V V K VYKNL  F E K   DDLFDRL T++LN++L+ELM GLTAKVFRTYNASITL+ +L       D+++ K+L+YN ANR VAILCNHQRA P TFEKSM+NL  KI  KK+  +E +   ++ +AE+    D   +   EK ++ LEK +E L ++  Q TD+E+NK VALGTSKLNYLDPRISIAWC +  VP+ KIY+KTQ ++F WA+ M  E+++F
Sbjct:    1 MLDHEYTTKEVFRKNFFNDWRKEMAVEEREVIKSLDKCDFTEIHRYFVDKAAARKVLSREEKQKLKEEAEKLQQEFGYCILDGHQEKIGNFKIEPPGLFRGRGDHPKMGMLKRRITPEDVVINCSRDSKIPEPPAGHQWKEVRSDNTVTWLAAWTESVQNSIKYIMLNPCSKLKGETAWQKFETARRLRGFVDEIRSQYRADWKSREMKTRQRAVALYFIDKLALRAGNEKEDGEAADTVGCCSLRVEHVQLHPEADGCQHVVEFDFLGKDCIRYYNRVPVEKPVYKNLQLFMENKDPRDDLFDRLTTTSLNKHLQELMDGLTAKVFRTYNASITLQEQLRALTRAEDSIAAKILSYNRANRVVAILCNHQRATPSTFEKSMQNLQTKIQAKKEQVAEARAELRRARAEHKAQGDGKSRSVLEKKRRLLEKLQEQLAQLSVQATDKEENKQVALGTSKLNYLDPRISIAWCKRFRVPVEKIYSKTQRERFAWALAMAGEDFEF 503
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Celegans
Match: top-1 (DNA topoisomerase 1 [Source:UniProtKB/Swiss-Prot;Acc:O17966])

HSP 1 Score: 603.208 bits (1554), Expect = 0.000e+0
Identity = 319/587 (54.34%), Postives = 397/587 (67.63%), Query Frame = 2 
Query:  341 RAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITL-------EVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKI----------------------DKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTT---ENYKF 2002
            +  G KW  L+H GP+F   Y PLPSHV F Y G+ MKL  ETEEIA FYA + DH+Y+  + FNKNFM+DW  VM+ +E+  I DL KCDF+ +  Y K+  E +KA+ KEEK K K + E  +  YG  ++D ++QK+ NF+IEPPG+FRGRG HPKMG +K+RI+PEDVIINC K+++IPKPP G KWKEVRHD TVTWL  W E+V    KY+MLN SS++KGEKD++KYETAR+L +KI  IR  YT+DF  +EMR+RQRA ALYFIDKLALRAGNEK  DE ADTVGCCSLRVEHI L       E D    E++V+FDFLGKDSIRY N VSV K+VYKNL  F EGK   DDLFDRL+T+TLN +LR LM GLT KVFRTYNASITL+ +L+      DNV+ K+L+YN ANRQVAILCNHQRA+ K F++SM+ L +KI                      +K +K Y  +K+  KKLK                                   +TD+++NK +ALGTSKLNY+DPRI++AWC K  VPL K++ KT  +KF+WA+DMT    E Y F
Sbjct:  181 KPAGVKWNSLQHCGPLFAPPYIPLPSHVHFKYGGEKMKLTLETEEIAQFYAGVLDHEYSTKEAFNKNFMKDWRKVMTVEERERIHDLKKCDFRAIDAYQKEQREIRKAMTKEEKLKIKEEKEAEVKIYGIAIIDGHRQKVANFRIEPPGVFRGRGGHPKMGLIKKRIMPEDVIINCGKDTEIPKPPPGHKWKEVRHDNTVTWLCSWTESVLGQNKYIMLNPSSKIKGEKDFEKYETARRLKKKIGGIRERYTDDFKSKEMRVRQRATALYFIDKLALRAGNEKDVDEAADTVGCCSLRVEHIKLFDSAKLNEDDKKEKEFVVEFDFLGKDSIRYFNRVSVEKRVYKNLKIFMEGKAPSDDLFDRLDTATLNDHLRSLMDGLTVKVFRTYNASITLQEQLIKLTNPKDNVAAKILSYNRANRQVAILCNHQRAVSKGFDESMQKLEQKIKDKKKEVKEAEAALKSARGAEKEKAQKKYDRLKEQLKKLKI---------------------------------SRTDKDENKQIALGTSKLNYIDPRITVAWCKKFEVPLEKVFTKTHREKFRWAIDMTNSSDEEYVF 734
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Celegans
Match: top-1 (DNA topoisomerase 1 [Source:UniProtKB/Swiss-Prot;Acc:O17966])

HSP 1 Score: 603.208 bits (1554), Expect = 0.000e+0
Identity = 319/587 (54.34%), Postives = 397/587 (67.63%), Query Frame = 2 
Query:  341 RAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITL-------EVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKI----------------------DKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTT---ENYKF 2002
            +  G KW  L+H GP+F   Y PLPSHV F Y G+ MKL  ETEEIA FYA + DH+Y+  + FNKNFM+DW  VM+ +E+  I DL KCDF+ +  Y K+  E +KA+ KEEK K K + E  +  YG  ++D ++QK+ NF+IEPPG+FRGRG HPKMG +K+RI+PEDVIINC K+++IPKPP G KWKEVRHD TVTWL  W E+V    KY+MLN SS++KGEKD++KYETAR+L +KI  IR  YT+DF  +EMR+RQRA ALYFIDKLALRAGNEK  DE ADTVGCCSLRVEHI L       E D    E++V+FDFLGKDSIRY N VSV K+VYKNL  F EGK   DDLFDRL+T+TLN +LR LM GLT KVFRTYNASITL+ +L+      DNV+ K+L+YN ANRQVAILCNHQRA+ K F++SM+ L +KI                      +K +K Y  +K+  KKLK                                   +TD+++NK +ALGTSKLNY+DPRI++AWC K  VPL K++ KT  +KF+WA+DMT    E Y F
Sbjct:  181 KPAGVKWNSLQHCGPLFAPPYIPLPSHVHFKYGGEKMKLTLETEEIAQFYAGVLDHEYSTKEAFNKNFMKDWRKVMTVEERERIHDLKKCDFRAIDAYQKEQREIRKAMTKEEKLKIKEEKEAEVKIYGIAIIDGHRQKVANFRIEPPGVFRGRGGHPKMGLIKKRIMPEDVIINCGKDTEIPKPPPGHKWKEVRHDNTVTWLCSWTESVLGQNKYIMLNPSSKIKGEKDFEKYETARRLKKKIGGIRERYTDDFKSKEMRVRQRATALYFIDKLALRAGNEKDVDEAADTVGCCSLRVEHIKLFDSAKLNEDDKKEKEFVVEFDFLGKDSIRYFNRVSVEKRVYKNLKIFMEGKAPSDDLFDRLDTATLNDHLRSLMDGLTVKVFRTYNASITLQEQLIKLTNPKDNVAAKILSYNRANRQVAILCNHQRAVSKGFDESMQKLEQKIKDKKKEVKEAEAALKSARGAEKEKAQKKYDRLKEQLKKLKI---------------------------------SRTDKDENKQIALGTSKLNYIDPRITVAWCKKFEVPLEKVFTKTHREKFRWAIDMTNSSDEEYVF 734
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Celegans
Match: top-1 (DNA topoisomerase 1 [Source:UniProtKB/Swiss-Prot;Acc:O17966])

HSP 1 Score: 603.208 bits (1554), Expect = 0.000e+0
Identity = 319/587 (54.34%), Postives = 397/587 (67.63%), Query Frame = 2 
Query:  341 RAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITL-------EVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKI----------------------DKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTT---ENYKF 2002
            +  G KW  L+H GP+F   Y PLPSHV F Y G+ MKL  ETEEIA FYA + DH+Y+  + FNKNFM+DW  VM+ +E+  I DL KCDF+ +  Y K+  E +KA+ KEEK K K + E  +  YG  ++D ++QK+ NF+IEPPG+FRGRG HPKMG +K+RI+PEDVIINC K+++IPKPP G KWKEVRHD TVTWL  W E+V    KY+MLN SS++KGEKD++KYETAR+L +KI  IR  YT+DF  +EMR+RQRA ALYFIDKLALRAGNEK  DE ADTVGCCSLRVEHI L       E D    E++V+FDFLGKDSIRY N VSV K+VYKNL  F EGK   DDLFDRL+T+TLN +LR LM GLT KVFRTYNASITL+ +L+      DNV+ K+L+YN ANRQVAILCNHQRA+ K F++SM+ L +KI                      +K +K Y  +K+  KKLK                                   +TD+++NK +ALGTSKLNY+DPRI++AWC K  VPL K++ KT  +KF+WA+DMT    E Y F
Sbjct:  253 KPAGVKWNSLQHCGPLFAPPYIPLPSHVHFKYGGEKMKLTLETEEIAQFYAGVLDHEYSTKEAFNKNFMKDWRKVMTVEERERIHDLKKCDFRAIDAYQKEQREIRKAMTKEEKLKIKEEKEAEVKIYGIAIIDGHRQKVANFRIEPPGVFRGRGGHPKMGLIKKRIMPEDVIINCGKDTEIPKPPPGHKWKEVRHDNTVTWLCSWTESVLGQNKYIMLNPSSKIKGEKDFEKYETARRLKKKIGGIRERYTDDFKSKEMRVRQRATALYFIDKLALRAGNEKDVDEAADTVGCCSLRVEHIKLFDSAKLNEDDKKEKEFVVEFDFLGKDSIRYFNRVSVEKRVYKNLKIFMEGKAPSDDLFDRLDTATLNDHLRSLMDGLTVKVFRTYNASITLQEQLIKLTNPKDNVAAKILSYNRANRQVAILCNHQRAVSKGFDESMQKLEQKIKDKKKEVKEAEAALKSARGAEKEKAQKKYDRLKEQLKKLKI---------------------------------SRTDKDENKQIALGTSKLNYIDPRITVAWCKKFEVPLEKVFTKTHREKFRWAIDMTNSSDEEYVF 806
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Celegans
Match: top-1 (DNA topoisomerase 1 [Source:UniProtKB/Swiss-Prot;Acc:O17966])

HSP 1 Score: 603.208 bits (1554), Expect = 0.000e+0
Identity = 319/587 (54.34%), Postives = 397/587 (67.63%), Query Frame = 2 
Query:  341 RAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITL-------EVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKI----------------------DKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTT---ENYKF 2002
            +  G KW  L+H GP+F   Y PLPSHV F Y G+ MKL  ETEEIA FYA + DH+Y+  + FNKNFM+DW  VM+ +E+  I DL KCDF+ +  Y K+  E +KA+ KEEK K K + E  +  YG  ++D ++QK+ NF+IEPPG+FRGRG HPKMG +K+RI+PEDVIINC K+++IPKPP G KWKEVRHD TVTWL  W E+V    KY+MLN SS++KGEKD++KYETAR+L +KI  IR  YT+DF  +EMR+RQRA ALYFIDKLALRAGNEK  DE ADTVGCCSLRVEHI L       E D    E++V+FDFLGKDSIRY N VSV K+VYKNL  F EGK   DDLFDRL+T+TLN +LR LM GLT KVFRTYNASITL+ +L+      DNV+ K+L+YN ANRQVAILCNHQRA+ K F++SM+ L +KI                      +K +K Y  +K+  KKLK                                   +TD+++NK +ALGTSKLNY+DPRI++AWC K  VPL K++ KT  +KF+WA+DMT    E Y F
Sbjct:  253 KPAGVKWNSLQHCGPLFAPPYIPLPSHVHFKYGGEKMKLTLETEEIAQFYAGVLDHEYSTKEAFNKNFMKDWRKVMTVEERERIHDLKKCDFRAIDAYQKEQREIRKAMTKEEKLKIKEEKEAEVKIYGIAIIDGHRQKVANFRIEPPGVFRGRGGHPKMGLIKKRIMPEDVIINCGKDTEIPKPPPGHKWKEVRHDNTVTWLCSWTESVLGQNKYIMLNPSSKIKGEKDFEKYETARRLKKKIGGIRERYTDDFKSKEMRVRQRATALYFIDKLALRAGNEKDVDEAADTVGCCSLRVEHIKLFDSAKLNEDDKKEKEFVVEFDFLGKDSIRYFNRVSVEKRVYKNLKIFMEGKAPSDDLFDRLDTATLNDHLRSLMDGLTVKVFRTYNASITLQEQLIKLTNPKDNVAAKILSYNRANRQVAILCNHQRAVSKGFDESMQKLEQKIKDKKKEVKEAEAALKSARGAEKEKAQKKYDRLKEQLKKLKI---------------------------------SRTDKDENKQIALGTSKLNYIDPRITVAWCKKFEVPLEKVFTKTHREKFRWAIDMTNSSDEEYVF 806
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Fly
Match: Top1 (gene:FBgn0004924 transcript:FBtr0100294)

HSP 1 Score: 640.573 bits (1651), Expect = 0.000e+0
Identity = 331/555 (59.64%), Postives = 413/555 (74.41%), Query Frame = 2 
Query:  341 RAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEY-SKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            RA G KW  LEH GP+F   YE +P +V+FYYDGK ++L  ETEE ATFYAKM +HDY   +VFN NF +D+   M+  E+ +IKD  KC+F+ M NYF+  SE +KA  KEEK   K +NE ++ ++G+CM+D +K+KIGNF++EPPGLFRGRG HPKMG +KRRI   DV INC K+SK+P PP G +WKEVRHD TVTWLA W ENVQ   KY+MLN SS+LKGEKD  KYETAR+L + IDKIR  Y +++  +EMR+RQRAVALYFIDKLALRAGNEK ED+ADTVGCCSLRVEH+ L  + +  E +V FDF GKDSIRY NEV V K+V+KNL  F E K+EGDDLFDRLNT  LN++L+ELM GLTAKVFRTYNAS TL+ +L      +  V  K+LAYN ANR VAILCNHQR++PK+ EKSMENL EKI  K       +++ +K ++EY S+ +  G         K+LE+ ++ L K+  Q+TDR++NK +ALGTSKLNYLDPRIS+AWC K++VP+ KI+NKTQ  KF WAV M  ENY+F
Sbjct:  436 RADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAK-------REAIEKCESEYHSRDEKKG---------KQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF 974
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Fly
Match: Top1 (gene:FBgn0004924 transcript:FBtr0074005)

HSP 1 Score: 640.573 bits (1651), Expect = 0.000e+0
Identity = 331/555 (59.64%), Postives = 413/555 (74.41%), Query Frame = 2 
Query:  341 RAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEY-SKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            RA G KW  LEH GP+F   YE +P +V+FYYDGK ++L  ETEE ATFYAKM +HDY   +VFN NF +D+   M+  E+ +IKD  KC+F+ M NYF+  SE +KA  KEEK   K +NE ++ ++G+CM+D +K+KIGNF++EPPGLFRGRG HPKMG +KRRI   DV INC K+SK+P PP G +WKEVRHD TVTWLA W ENVQ   KY+MLN SS+LKGEKD  KYETAR+L + IDKIR  Y +++  +EMR+RQRAVALYFIDKLALRAGNEK ED+ADTVGCCSLRVEH+ L  + +  E +V FDF GKDSIRY NEV V K+V+KNL  F E K+EGDDLFDRLNT  LN++L+ELM GLTAKVFRTYNAS TL+ +L      +  V  K+LAYN ANR VAILCNHQR++PK+ EKSMENL EKI  K       +++ +K ++EY S+ +  G         K+LE+ ++ L K+  Q+TDR++NK +ALGTSKLNYLDPRIS+AWC K++VP+ KI+NKTQ  KF WAV M  ENY+F
Sbjct:  436 RADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAK-------REAIEKCESEYHSRDEKKG---------KQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF 974
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Fly
Match: Top1 (gene:FBgn0004924 transcript:FBtr0308699)

HSP 1 Score: 639.802 bits (1649), Expect = 0.000e+0
Identity = 331/555 (59.64%), Postives = 413/555 (74.41%), Query Frame = 2 
Query:  341 RAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEY-SKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            RA G KW  LEH GP+F   YE +P +V+FYYDGK ++L  ETEE ATFYAKM +HDY   +VFN NF +D+   M+  E+ +IKD  KC+F+ M NYF+  SE +KA  KEEK   K +NE ++ ++G+CM+D +K+KIGNF++EPPGLFRGRG HPKMG +KRRI   DV INC K+SK+P PP G +WKEVRHD TVTWLA W ENVQ   KY+MLN SS+LKGEKD  KYETAR+L + IDKIR  Y +++  +EMR+RQRAVALYFIDKLALRAGNEK ED+ADTVGCCSLRVEH+ L  + +  E +V FDF GKDSIRY NEV V K+V+KNL  F E K+EGDDLFDRLNT  LN++L+ELM GLTAKVFRTYNAS TL+ +L      +  V  K+LAYN ANR VAILCNHQR++PK+ EKSMENL EKI  K       +++ +K ++EY S+ +  G         K+LE+ ++ L K+  Q+TDR++NK +ALGTSKLNYLDPRIS+AWC K++VP+ KI+NKTQ  KF WAV M  ENY+F
Sbjct:  432 RADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAK-------REAIEKCESEYHSRDEKKG---------KQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF 970
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Fly
Match: Top1 (gene:FBgn0004924 transcript:FBtr0074006)

HSP 1 Score: 637.876 bits (1644), Expect = 0.000e+0
Identity = 331/555 (59.64%), Postives = 413/555 (74.41%), Query Frame = 2 
Query:  341 RAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEY-SKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            RA G KW  LEH GP+F   YE +P +V+FYYDGK ++L  ETEE ATFYAKM +HDY   +VFN NF +D+   M+  E+ +IKD  KC+F+ M NYF+  SE +KA  KEEK   K +NE ++ ++G+CM+D +K+KIGNF++EPPGLFRGRG HPKMG +KRRI   DV INC K+SK+P PP G +WKEVRHD TVTWLA W ENVQ   KY+MLN SS+LKGEKD  KYETAR+L + IDKIR  Y +++  +EMR+RQRAVALYFIDKLALRAGNEK ED+ADTVGCCSLRVEH+ L  + +  E +V FDF GKDSIRY NEV V K+V+KNL  F E K+EGDDLFDRLNT  LN++L+ELM GLTAKVFRTYNAS TL+ +L      +  V  K+LAYN ANR VAILCNHQR++PK+ EKSMENL EKI  K       +++ +K ++EY S+ +  G         K+LE+ ++ L K+  Q+TDR++NK +ALGTSKLNYLDPRIS+AWC K++VP+ KI+NKTQ  KF WAV M  ENY+F
Sbjct:   43 RADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAK-------REAIEKCESEYHSRDEKKG---------KQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF 581
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Fly
Match: Top1 (gene:FBgn0004924 transcript:FBtr0340434)

HSP 1 Score: 637.876 bits (1644), Expect = 0.000e+0
Identity = 331/555 (59.64%), Postives = 413/555 (74.41%), Query Frame = 2 
Query:  341 RAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEY-SKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            RA G KW  LEH GP+F   YE +P +V+FYYDGK ++L  ETEE ATFYAKM +HDY   +VFN NF +D+   M+  E+ +IKD  KC+F+ M NYF+  SE +KA  KEEK   K +NE ++ ++G+CM+D +K+KIGNF++EPPGLFRGRG HPKMG +KRRI   DV INC K+SK+P PP G +WKEVRHD TVTWLA W ENVQ   KY+MLN SS+LKGEKD  KYETAR+L + IDKIR  Y +++  +EMR+RQRAVALYFIDKLALRAGNEK ED+ADTVGCCSLRVEH+ L  + +  E +V FDF GKDSIRY NEV V K+V+KNL  F E K+EGDDLFDRLNT  LN++L+ELM GLTAKVFRTYNAS TL+ +L      +  V  K+LAYN ANR VAILCNHQR++PK+ EKSMENL EKI  K       +++ +K ++EY S+ +  G         K+LE+ ++ L K+  Q+TDR++NK +ALGTSKLNYLDPRIS+AWC K++VP+ KI+NKTQ  KF WAV M  ENY+F
Sbjct:   55 RADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEAATFYAKMLNHDYCTKEVFNNNFFKDFRKSMTPREREIIKDFRKCNFQEMFNYFQAESEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGMIKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPSSKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNEKDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLELFMEHKKEGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATVPEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAK-------REAIEKCESEYHSRDEKKG---------KQLERLRDQLKKLELQETDRDENKTIALGTSKLNYLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF 593
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Zebrafish
Match: top1l (DNA topoisomerase I, like [Source:ZFIN;Acc:ZDB-GENE-060616-217])

HSP 1 Score: 691.804 bits (1784), Expect = 0.000e+0
Identity = 351/552 (63.59%), Postives = 427/552 (77.36%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLPS+VKFYYDGKHMKL P  EE+ATF++KM DH+YT  D+F KNF +DW   M+ +EKS I DL+KCDF  M  YFK  SE +KA+ KEEK K K +NE I+ +YG+C+MD++K++I NF+IEPPGLFRGRG+HPKMG +KRRI PED+IINCSK+SK P PP G KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +D+IR  Y +D+  +EM+IRQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEH+TL  +    E++V+FDFLGKDSIRY N+V V K+V+KNL  F E K+  DDLFDRLNTS LN++L+ELM GLTAKVFRTYNASITL+++L       DNV  K+L+YN ANR VAILCNHQRA PKTFEKSM+NL  KID KK+   E K   K  KA+    KD   K+  E  KK L++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC K  +P+ KIYNKTQ +KF WA+DM  ++Y+F
Sbjct:  207 GVKWKFLEHKGPVFAPPYEPLPSNVKFYYDGKHMKLSPNAEEVATFFSKMLDHEYTTKDIFRKNFFKDWRKEMTSEEKSKITDLNKCDFSNMSEYFKAQSEARKAMTKEEKLKIKEENERILQEYGFCIMDNHKERIANFRIEPPGLFRGRGDHPKMGMLKRRIRPEDIIINCSKDSKHPTPPPGTKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDRIRSQYRDDWKSKEMKIRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHLTLHPEMDGQEFVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMEDKEPEDDLFDRLNTSILNKHLQELMDGLTAKVFRTYNASITLQQQLNELTNSEDNVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMQNLQTKIDAKKEQLVEAKRELKGAKADAKVRKDEKFKKAVEAKKKALQRVEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKFGIPIEKIYNKTQREKFAWAIDMAEKDYEF 758
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Zebrafish
Match: top1 (DNA topoisomerase I [Source:ZFIN;Acc:ZDB-GENE-030131-5390])

HSP 1 Score: 671.389 bits (1731), Expect = 0.000e+0
Identity = 343/552 (62.14%), Postives = 422/552 (76.45%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLP H+KFYYDG  MKL P  EE+ATFYAKM +H+YT N++F KNF +DW   M+ +EKS I +L KCDF  M  YFK  SE +K + KEEK+KNK +NE I+ +YG+C+MD+++++IGNF+IEPPGLFRGRG+HPKMG +KRRI PED+IINCSK+S  PK P G +WKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +++IR  Y  D+  +EMRIRQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L       +Y+V+FDFLGKDSIRY N++ V K+V+KNL  F E KQ  DDLFDRLNTS LN+YL+ELM GLTAKVFRTYNASITL+++L N+    +N+  K+L+YN ANR VA+LCNHQRA PKTFEKSM+NL  KID+KKK  S  K   K  KA+     D   K+  E  KK +++  E L K+  Q TDRE+NK +ALGTSKLNYLDPRIS++WC K  VP+ KIY+KTQ  KF WA+DM  E+++F
Sbjct:   11 GSKWRFLEHKGPVFPPPYEPLPGHIKFYYDGNAMKLSPAAEEVATFYAKMLEHEYTTNEIFRKNFFKDWRKEMTSEEKSKITELKKCDFTQMHEYFKAQSEARKQMSKEEKQKNKEENERILQEYGFCLMDNHRERIGNFRIEPPGLFRGRGDHPKMGKLKRRIRPEDIIINCSKDSVQPKAPPGTRWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVERIRAQYREDWRSKEMRIRQRAVALYFIDKLALRAGNEKDEGETADTVGCCSLRVEHIKLHPQLDGQDYVVEFDFLGKDSIRYYNKIPVEKRVFKNLQLFLENKQSEDDLFDRLNTSILNKYLQELMDGLTAKVFRTYNASITLQQQL-NELTTGENIPEKILSYNRANRAVALLCNHQRAPPKTFEKSMQNLQTKIDEKKKQLSAAKKEHKAAKADAKALHDEKSKKAVETKKKAVQRISEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVSWCKKWGVPIEKIYSKTQRDKFAWAIDMADEDFEF 561
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Zebrafish
Match: zgc:173742 (zgc:173742 [Source:ZFIN;Acc:ZDB-GENE-030131-6489])

HSP 1 Score: 551.977 bits (1421), Expect = 0.000e+0
Identity = 276/454 (60.79%), Postives = 342/454 (75.33%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKK 1708
            G KW+FLEH GP F  AY+PLP  VKFYY+G+ +KL    EE+A ++A+M DH+YT  +VF  NF +DW   M+ +E+ +I DL KCDF  +    K+  E +K L KEEK  NK  N++I+++YG+C++D ++++IGNFKIEPPGLFRGRG+HPK G +K+RI PEDVIINCSK S+IP+ PAG KWKEVRHD TVTWLA W ENVQ + KYVMLNA+S+LKGEKDW+KYE ARKL   +D IR  Y  D   ++M  RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHITL       + +V+FDFLGKD IRY N+V V K+V+KNL  F E KQ GDDLFDRLNT++LN++L  LMPGLTAKVFRTYNASITL+++L     + DNV  K+L+YN ANR VA+LCNHQRA PK+F++SM NL  K+    KD+  +K   KK
Sbjct:  184 GIKWKFLEHKGPYFPPAYQPLPDDVKFYYNGEPVKLSLPAEEVALYFAQMLDHEYTTKEVFRNNFFKDWRKEMTLEERQLIMDLKKCDFGELHAMHKEKVEARKNLSKEEKLVNKEANQKIVDEYGFCVLDHHRERIGNFKIEPPGLFRGRGDHPKQGMLKKRIRPEDVIINCSKESRIPEAPAGHKWKEVRHDKTVTWLASWTENVQGSCKYVMLNANSKLKGEKDWEKYEVARKLKTCVDAIRTQYQEDLKSKQMGTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHDALDDQKCVVEFDFLGKDCIRYYNKVPVTKRVFKNLKLFMENKQGGDDLFDRLNTASLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNMKDNVPEKLLSYNRANRAVAVLCNHQRAPPKSFDQSMANLQAKVKLHMKDWIVMKQFKKK 637
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Zebrafish
Match: top1 (DNA topoisomerase I [Source:ZFIN;Acc:ZDB-GENE-030131-5390])

HSP 1 Score: 524.628 bits (1350), Expect = 5.820e-178
Identity = 255/389 (65.55%), Postives = 310/389 (79.69%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLEREL 1513
            G KW+FLEH GP+F   YEPLP H+KFYYDG  MKL P  EE+ATFYAKM +H+YT N++F KNF +DW   M+ +EKS I +L KCDF  M  YFK  SE +K + KEEK+KNK +NE I+ +YG+C+MD+++++IGNF+IEPPGLFRGRG+HPKMG +KRRI PED+IINCSK+S  PK P G +WKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +++IR  Y  D+  +EMRIRQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L       +Y+V+FDFLGKDSIRY N++ V K+V+KNL  F E KQ  DDLFDRLNTS LN+YL+ELM GLTAKVFRTYNASITL+++L
Sbjct:  121 GSKWRFLEHKGPVFPPPYEPLPGHIKFYYDGNAMKLSPAAEEVATFYAKMLEHEYTTNEIFRKNFFKDWRKEMTSEEKSKITELKKCDFTQMHEYFKAQSEARKQMSKEEKQKNKEENERILQEYGFCLMDNHRERIGNFRIEPPGLFRGRGDHPKMGKLKRRIRPEDIIINCSKDSVQPKAPPGTRWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVERIRAQYREDWRSKEMRIRQRAVALYFIDKLALRAGNEKDEGETADTVGCCSLRVEHIKLHPQLDGQDYVVEFDFLGKDSIRYYNKIPVEKRVFKNLQLFLENKQSEDDLFDRLNTSILNKYLQELMDGLTAKVFRTYNASITLQQQL 509
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Zebrafish
Match: top1mt (DNA topoisomerase I mitochondrial [Source:ZFIN;Acc:ZDB-GENE-040624-2])

HSP 1 Score: 501.516 bits (1290), Expect = 5.837e-169
Identity = 273/551 (49.55%), Postives = 358/551 (64.97%), Query Frame = 2 
Query:  356 KWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADN--VSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            KW  LEH GP F   YEPLP+++ FYYDG+ +KL P TEEIATFYAKM DH+YT  +VF KNF +DW   M+K+E+ +I  LSKCDF  +  YF + +E+KK + KEEK+  K +   ++ ++GYC++D +++KIGNFK+EPPGLFRGRG HPKMG +K+RI PEDV INCSK+SKIP+PPAG KWK V+HD TVTWLA W EN+Q   KY+MLN SS+LK                                             +       GN+ G D    +      + HI L       +++V+FDFLGKDSIRY N+V V K+V+KNL  F + K   DDLFDR++T  LN+ L E M GLTAKVFRT+NAS TL+ +L   K+ +D+  V  K+L+YN ANR VAILCNHQRA PKTFEKSM+ L EKI KKK+   E +   K+ K     +      +  EK +K +++  + L K+  Q+TDRE+NK +ALGTSKLNYLDPRI++AWC K+ VP+ KI+NKT  +KF WA+DMT E+++F
Sbjct:   52 KWSSLEHKGPYFAPDYEPLPANICFYYDGQQVKLSPLTEEIATFYAKMLDHEYTTKEVFQKNFFKDWRKEMTKEERKLITTLSKCDFTHIHKYFVEKAEEKKNMTKEEKQVLKDEAARLMEEFGYCLLDGHREKIGNFKLEPPGLFRGRGEHPKMGRLKKRIQPEDVAINCSKDSKIPEPPAGHKWKRVQHDNTVTWLASWLENIQKRPKYIMLNPSSKLK---------------------------------------------VGNYDGGVGNDGGSDYDGVI------ILHIRLHKHLDGLDHVVEFDFLGKDSIRYYNKVPVEKQVFKNLKLFIKHKDPEDDLFDRISTVNLNKALTESMSGLTAKVFRTFNASTTLQDQL--NKLTSDDGTVEEKILSYNRANRAVAILCNHQRAAPKTFEKSMQLLQEKIQKKKEQIEEARKELKEAKQVDKDTPSDKSSKLVEKKQKAVQRLCDQLKKLQLQETDREENKVIALGTSKLNYLDPRITVAWCKKHQVPIEKIFNKTHREKFAWAIDMTDESFQF 549
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Xenopus
Match: TOP1 (DNA topoisomerase I, gene 1 [Source:NCBI gene;Acc:100496521])

HSP 1 Score: 666.381 bits (1718), Expect = 0.000e+0
Identity = 337/554 (60.83%), Postives = 421/554 (75.99%), Query Frame = 2 
Query:  344 AGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            A G KW+FLEH GP+F   YEPLP +VKFYYDG  +KL P+ EE+ATF+AKM DH+YT  D+F KNF +DW   M+ DE+++I +L KCDF  M  YFK+ SE +K + KEEK+K K +NE ++ +YG+C+MD+++++I NF+IEPPGLFRGRG+HPKMG +K+RI+PED+IINCSK+SK P  PAG KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L   ++KIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    E++V+FDF GKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNTS LN++L++LM GLTAKVFRTYNASITL+++L       DNV  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK    + +   K  KA+    +D   K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC K  +P+ KIYNKTQ +KF WA+DM  E++ F
Sbjct:  156 ADGIKWKFLEHKGPVFAPPYEPLPDNVKFYYDGNLVKLSPKAEEVATFFAKMLDHEYTTKDIFRKNFFKDWKKEMTADERNLITNLGKCDFNAMSQYFKEQSEARKNMTKEEKQKIKEENERLLQEYGFCVMDNHRERIANFRIEPPGLFRGRGDHPKMGKLKKRIMPEDIIINCSKDSKYPVAPAGHKWKEVRHDGKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKMCVEKIRNTYKEDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLFQELDGQEFVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPDDDLFDRLNTSILNKHLQDLMEGLTAKVFRTYNASITLQQQLNELTNGDDNVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQGKIDAKKDQLMDARRELKSAKADAKVKRDEKSKKLVESKKKAVQRVEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKYGIPIEKIYNKTQREKFAWALDMADEDFAF 709
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Xenopus
Match: TOP1 (DNA topoisomerase I, gene 1 [Source:NCBI gene;Acc:100496521])

HSP 1 Score: 665.611 bits (1716), Expect = 0.000e+0
Identity = 337/554 (60.83%), Postives = 421/554 (75.99%), Query Frame = 2 
Query:  344 AGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            A G KW+FLEH GP+F   YEPLP +VKFYYDG  +KL P+ EE+ATF+AKM DH+YT  D+F KNF +DW   M+ DE+++I +L KCDF  M  YFK+ SE +K + KEEK+K K +NE ++ +YG+C+MD+++++I NF+IEPPGLFRGRG+HPKMG +K+RI+PED+IINCSK+SK P  PAG KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L   ++KIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    E++V+FDF GKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNTS LN++L++LM GLTAKVFRTYNASITL+++L       DNV  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK    + +   K  KA+    +D   K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC K  +P+ KIYNKTQ +KF WA+DM  E++ F
Sbjct:   32 ADGIKWKFLEHKGPVFAPPYEPLPDNVKFYYDGNLVKLSPKAEEVATFFAKMLDHEYTTKDIFRKNFFKDWKKEMTADERNLITNLGKCDFNAMSQYFKEQSEARKNMTKEEKQKIKEENERLLQEYGFCVMDNHRERIANFRIEPPGLFRGRGDHPKMGKLKKRIMPEDIIINCSKDSKYPVAPAGHKWKEVRHDGKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKMCVEKIRNTYKEDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLFQELDGQEFVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPDDDLFDRLNTSILNKHLQDLMEGLTAKVFRTYNASITLQQQLNELTNGDDNVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQGKIDAKKDQLMDARRELKSAKADAKVKRDEKSKKLVESKKKAVQRVEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKYGIPIEKIYNKTQREKFAWALDMADEDFAF 585
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Xenopus
Match: TOP1 (DNA topoisomerase I, gene 1 [Source:NCBI gene;Acc:100496521])

HSP 1 Score: 665.611 bits (1716), Expect = 0.000e+0
Identity = 337/554 (60.83%), Postives = 421/554 (75.99%), Query Frame = 2 
Query:  344 AGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            A G KW+FLEH GP+F   YEPLP +VKFYYDG  +KL P+ EE+ATF+AKM DH+YT  D+F KNF +DW   M+ DE+++I +L KCDF  M  YFK+ SE +K + KEEK+K K +NE ++ +YG+C+MD+++++I NF+IEPPGLFRGRG+HPKMG +K+RI+PED+IINCSK+SK P  PAG KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L   ++KIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    E++V+FDF GKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNTS LN++L++LM GLTAKVFRTYNASITL+++L       DNV  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK    + +   K  KA+    +D   K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC K  +P+ KIYNKTQ +KF WA+DM  E++ F
Sbjct:  153 ADGIKWKFLEHKGPVFAPPYEPLPDNVKFYYDGNLVKLSPKAEEVATFFAKMLDHEYTTKDIFRKNFFKDWKKEMTADERNLITNLGKCDFNAMSQYFKEQSEARKNMTKEEKQKIKEENERLLQEYGFCVMDNHRERIANFRIEPPGLFRGRGDHPKMGKLKKRIMPEDIIINCSKDSKYPVAPAGHKWKEVRHDGKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKMCVEKIRNTYKEDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLFQELDGQEFVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPDDDLFDRLNTSILNKHLQDLMEGLTAKVFRTYNASITLQQQLNELTNGDDNVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQGKIDAKKDQLMDARRELKSAKADAKVKRDEKSKKLVESKKKAVQRVEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKYGIPIEKIYNKTQREKFAWALDMADEDFAF 706
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Xenopus
Match: TOP1 (DNA topoisomerase I, gene 1 [Source:NCBI gene;Acc:100496521])

HSP 1 Score: 664.84 bits (1714), Expect = 0.000e+0
Identity = 337/554 (60.83%), Postives = 421/554 (75.99%), Query Frame = 2 
Query:  344 AGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            A G KW+FLEH GP+F   YEPLP +VKFYYDG  +KL P+ EE+ATF+AKM DH+YT  D+F KNF +DW   M+ DE+++I +L KCDF  M  YFK+ SE +K + KEEK+K K +NE ++ +YG+C+MD+++++I NF+IEPPGLFRGRG+HPKMG +K+RI+PED+IINCSK+SK P  PAG KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L   ++KIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    E++V+FDF GKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNTS LN++L++LM GLTAKVFRTYNASITL+++L       DNV  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK    + +   K  KA+    +D   K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC K  +P+ KIYNKTQ +KF WA+DM  E++ F
Sbjct:  185 ADGIKWKFLEHKGPVFAPPYEPLPDNVKFYYDGNLVKLSPKAEEVATFFAKMLDHEYTTKDIFRKNFFKDWKKEMTADERNLITNLGKCDFNAMSQYFKEQSEARKNMTKEEKQKIKEENERLLQEYGFCVMDNHRERIANFRIEPPGLFRGRGDHPKMGKLKKRIMPEDIIINCSKDSKYPVAPAGHKWKEVRHDGKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKMCVEKIRNTYKEDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLFQELDGQEFVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPDDDLFDRLNTSILNKHLQDLMEGLTAKVFRTYNASITLQQQLNELTNGDDNVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQGKIDAKKDQLMDARRELKSAKADAKVKRDEKSKKLVESKKKAVQRVEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKYGIPIEKIYNKTQREKFAWALDMADEDFAF 738
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Xenopus
Match: TOP1 (DNA topoisomerase I, gene 1 [Source:NCBI gene;Acc:100496521])

HSP 1 Score: 664.455 bits (1713), Expect = 0.000e+0
Identity = 337/554 (60.83%), Postives = 421/554 (75.99%), Query Frame = 2 
Query:  344 AGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            A G KW+FLEH GP+F   YEPLP +VKFYYDG  +KL P+ EE+ATF+AKM DH+YT  D+F KNF +DW   M+ DE+++I +L KCDF  M  YFK+ SE +K + KEEK+K K +NE ++ +YG+C+MD+++++I NF+IEPPGLFRGRG+HPKMG +K+RI+PED+IINCSK+SK P  PAG KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L   ++KIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    E++V+FDF GKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNTS LN++L++LM GLTAKVFRTYNASITL+++L       DNV  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK    + +   K  KA+    +D   K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC K  +P+ KIYNKTQ +KF WA+DM  E++ F
Sbjct:  101 ADGIKWKFLEHKGPVFAPPYEPLPDNVKFYYDGNLVKLSPKAEEVATFFAKMLDHEYTTKDIFRKNFFKDWKKEMTADERNLITNLGKCDFNAMSQYFKEQSEARKNMTKEEKQKIKEENERLLQEYGFCVMDNHRERIANFRIEPPGLFRGRGDHPKMGKLKKRIMPEDIIINCSKDSKYPVAPAGHKWKEVRHDGKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKMCVEKIRNTYKEDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLFQELDGQEFVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPDDDLFDRLNTSILNKHLQDLMEGLTAKVFRTYNASITLQQQLNELTNGDDNVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQGKIDAKKDQLMDARRELKSAKADAKVKRDEKSKKLVESKKKAVQRVEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKYGIPIEKIYNKTQREKFAWALDMADEDFAF 654
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Mouse
Match: Top1 (topoisomerase (DNA) I [Source:MGI Symbol;Acc:MGI:98788])

HSP 1 Score: 683.715 bits (1763), Expect = 0.000e+0
Identity = 344/552 (62.32%), Postives = 424/552 (76.81%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLP  VKFYYDGK MKL P+ EE+ATF+AKM DH+YT  ++F KNF +DW   M+ DEK+ I +LSKCDF  M  YFK  SE +K + KEEK K K +NE+++ +YG+C+MD+++++I NFKIEPPGLFRGRGNHPKMG +KRRI+PED+IINCSK++K+P PP G KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +DKIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    EY+V+FDF GKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNT  LN++L++LM GLTAKVFRTYNASITL+++L       +NV  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK   ++ +   K  KA+    KD+  K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRI++AWC K  VP+ KIYNKTQ +KF WA+DMT E+Y+F
Sbjct:  216 GIKWKFLEHKGPVFAPPYEPLPESVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNDEKNTITNLSKCDFTQMSQYFKAQSEARKQMSKEEKLKIKEENEKLLKEYGFCVMDNHRERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF 767
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Mouse
Match: Top1mt (DNA topoisomerase 1, mitochondrial [Source:MGI Symbol;Acc:MGI:1920210])

HSP 1 Score: 605.905 bits (1561), Expect = 0.000e+0
Identity = 306/550 (55.64%), Postives = 403/550 (73.27%), Query Frame = 2 
Query:  356 KWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            KW+ LEH GP F  AYEPLP  V F YDGK +KL    EE+ATFY KM   + T  +VF +NF  DW   M+ +E+ +I  L KCDF  +  +F + +E ++ LP+E+K+K K + E++  ++GYC++D +++KIGNFK EPPGLFRGRG+HPKMG +KRR++PEDV+INCS++SKIP+PPAG +WKEVR D TV WLA W E++QN+FKYV+LN SS+ KGE DW+KYE AR+L   +DKIR  Y  D+   EM+ RQ AVALYFIDKLALR GNEK E E ADTVGCCSLRVEHI L   +   E++V+ DFLGKDSIRY+N V+V K V++NL  F E K   DDLFD L TS+LN++L++LM GLTAKVFRTYNAS+TL+ +L       D+V+ KVLAYN ANR VA+LCNHQRA+PKTFEKSM+ L +KI+ KK   +E +   +K + +     DS   + + + +++L K +E L ++  + TD+E+NK VALGT+KLNYLDPRISIAWC +  VP+ KIYNKTQ ++F WA +   E+++F
Sbjct:   45 KWRQLEHKGPYFTPAYEPLPDGVHFLYDGKPLKLSLAAEEVATFYGKMLHLECTSKEVFRRNFFSDWRKEMTPEERRLITHLDKCDFTEIHKHFLERAEARRTLPREQKQKLKEEAEKLQQEFGYCVLDGHREKIGNFKTEPPGLFRGRGDHPKMGMLKRRVMPEDVVINCSRDSKIPEPPAGHQWKEVRSDNTVMWLAAWVEHIQNSFKYVILNPSSKPKGEMDWQKYEVARRLKGVVDKIRAQYQADWKSPEMKKRQLAVALYFIDKLALRTGNEKEEGETADTVGCCSLRVEHIRLHAPAGGQEHVVELDFLGKDSIRYKNHVTVEKLVFQNLQHFMEDKDPRDDLFDALTTSSLNKHLQDLMEGLTAKVFRTYNASVTLQEQLRVLTRAEDSVTCKVLAYNRANRAVAVLCNHQRAVPKTFEKSMQTLQKKIETKKAQVAEAQVELQKAETDLRMRGDSK-PKSFLQKQRRLLKLEEQLARLCAKATDKEENKQVALGTAKLNYLDPRISIAWCKRFGVPVEKIYNKTQRERFAWAFNQAGEDFEF 593
[Close]
BLAST of DNA topoisomerase I vs. UniProt/SwissProt
Match: sp|P11387|TOP1_HUMAN (DNA topoisomerase 1 OS=Homo sapiens OX=9606 GN=TOP1 PE=1 SV=2)

HSP 1 Score: 684.485 bits (1765), Expect = 0.000e+0
Identity = 342/552 (61.96%), Postives = 427/552 (77.36%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLP +VKFYYDGK MKL P+ EE+ATF+AKM DH+YT  ++F KNF +DW   M+ +EK++I +LSKCDF  M  YFK  +E +K + KEEK K K +NE+++ +YG+C+MD++K++I NFKIEPPGLFRGRGNHPKMG +KRRI+PED+IINCSK++K+P PP G KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +DKIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    EY+V+FDFLGKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNT  LN++L++LM GLTAKVFRTYNASITL+++L       +N+  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK+  ++ +   K  KA+    KD+  K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRI++AWC K  VP+ KIYNKTQ +KF WA+DM  E+Y+F
Sbjct:  214 GIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF 765
[Close]
BLAST of DNA topoisomerase I vs. UniProt/SwissProt
Match: sp|Q7YR26|TOP1_CHLAE (DNA topoisomerase 1 OS=Chlorocebus aethiops OX=9534 GN=TOP1 PE=2 SV=1)

HSP 1 Score: 684.485 bits (1765), Expect = 0.000e+0
Identity = 342/552 (61.96%), Postives = 426/552 (77.17%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLP  VKFYYDGK MKL P+ EE+ATF+AKM DH+YT  ++F KNF +DW   M+ +EK++I +LSKCDF  M  YFK  +E +K + KEEK K K +NE+++ +YG+C+MD++K++I NFKIEPPGLFRGRGNHPKMG +KRRI+PED+IINCSK++K+P PP G KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +DKIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    EY+V+FDFLGKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNT  LN++L++LM GLTAKVFRTYNASITL+++L       +N+  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK+  ++ +   K  KA+    KD+  K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRI++AWC K  VP+ KIYNKTQ +KF WA+DM  E+Y+F
Sbjct:  216 GIKWKFLEHKGPVFAPPYEPLPDSVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMADEDYEF 767
[Close]
BLAST of DNA topoisomerase I vs. UniProt/SwissProt
Match: sp|Q04750|TOP1_MOUSE (DNA topoisomerase 1 OS=Mus musculus OX=10090 GN=Top1 PE=1 SV=2)

HSP 1 Score: 683.715 bits (1763), Expect = 0.000e+0
Identity = 344/552 (62.32%), Postives = 424/552 (76.81%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLP  VKFYYDGK MKL P+ EE+ATF+AKM DH+YT  ++F KNF +DW   M+ DEK+ I +LSKCDF  M  YFK  SE +K + KEEK K K +NE+++ +YG+C+MD+++++I NFKIEPPGLFRGRGNHPKMG +KRRI+PED+IINCSK++K+P PP G KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +DKIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    EY+V+FDF GKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNT  LN++L++LM GLTAKVFRTYNASITL+++L       +NV  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK   ++ +   K  KA+    KD+  K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRI++AWC K  VP+ KIYNKTQ +KF WA+DMT E+Y+F
Sbjct:  216 GIKWKFLEHKGPVFAPPYEPLPESVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNDEKNTITNLSKCDFTQMSQYFKAQSEARKQMSKEEKLKIKEENEKLLKEYGFCVMDNHRERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF 767
[Close]
BLAST of DNA topoisomerase I vs. UniProt/SwissProt
Match: sp|Q9WUL0|TOP1_RAT (DNA topoisomerase 1 OS=Rattus norvegicus OX=10116 GN=Top1 PE=1 SV=1)

HSP 1 Score: 683.33 bits (1762), Expect = 0.000e+0
Identity = 344/552 (62.32%), Postives = 424/552 (76.81%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLP  VKFYYDGK MKL P+ EE+ATF+AKM DH+YT  ++F KNF +DW   M+ DEK+ I +LSKCDF  M  YFK  SE +K + KEEK K K +NE+++ +YG+C+MD+++++I NFKIEPPGLFRGRGNHPKMG +KRRI+PED+IINCSK++K+P PP G KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +DKIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    EY+V+FDF GKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNT  LN++L++LM GLTAKVFRTYNASITL+++L       +NV  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK   ++ +   K  KA+    KD+  K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRI++AWC K  VP+ KIYNKTQ +KF WA+DMT E+Y+F
Sbjct:  216 GIKWKFLEHKGPVFAPPYEPLPEGVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNDEKNTITNLSKCDFTQMSQYFKAQSEARKQMSKEEKLKIKEENEKLLKEYGFCVMDNHRERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARKDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF 767
[Close]
BLAST of DNA topoisomerase I vs. UniProt/SwissProt
Match: sp|Q07050|TOP1_CRIGR (DNA topoisomerase 1 OS=Cricetulus griseus OX=10029 GN=TOP1 PE=2 SV=1)

HSP 1 Score: 681.789 bits (1758), Expect = 0.000e+0
Identity = 344/552 (62.32%), Postives = 424/552 (76.81%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLP  VKFYYDGK MKL P+ EE+ATF+AKM DH+YT  ++F KNF +DW   M+ DEK+VI +LSKCDF  M  YFK  SE +K + KEEK K K +NE+++ +YG+C+MD+++++I NFKIEPPGLFRGRGNHPKMG +KRRI+PED+IINCSK++K+P PP G KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +DKIR  Y  D+  +EM++RQRAVALYFIDKLALRAGNEK E E ADTV CCSLRVEHI L  +    EY+V+FDF GKDSIRY N+V V K+V+KNL  F E KQ  DDLFDRLNT  LN++L++LM GLTAKVFRTYNASITL+++L       +NV  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID KK   ++ +   K  KA+    KD+  K+  E  KK +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRI++AWC K  VP+ KIYNKTQ +KF WA+DMT E+Y+F
Sbjct:  216 GIKWKFLEHKGPVFAPPYEPLPEGVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNDEKNVITNLSKCDFTQMSQYFKDQSEARKQMSKEEKLKIKEENEKLLKEYGFCVMDNHRERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVSCCSLRVEHINLHPELDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF 767
[Close]
BLAST of DNA topoisomerase I vs. TrEMBL
Match: A0A0X3NSW1 (DNA topoisomerase I OS=Schistocephalus solidus OX=70667 GN=TR136369 PE=3 SV=1)

HSP 1 Score: 747.658 bits (1929), Expect = 0.000e+0
Identity = 374/557 (67.15%), Postives = 447/557 (80.25%), Query Frame = 2 
Query:  356 KWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKFQDLNSDKS 2026
            KW FLEH GPMF   YEPLPS V+FYYDG  M+L PE EE+A FYA+M DH+YT  ++FN NF RDW   M+++E+ VIK L KCDF+PM  YFK+ SE++K + KEEK+K K  N+ I  +YG+C +D +KQ+I NFKIEPP LFRGRG+HPKMG +K+RI+PED+IINCSK+SKIP PP G +WKEVRHD TVTWLACW EN+Q+NFKY+MLNASSRLKGEKDWKKYETARKLH+ +DKIR DY  DF  +EMRIRQRAVALYFIDKLALRAGNEK EDEADTVGCCSLR EH+TL  +     ++V+FDFLGKDSIRYQN V VPK+V+KNL  F + KQ  DDLFDRLNTS LNQYLRELM GLTAKVFRTYNAS TLE +L       D+V+ K+L+YN ANR VA+LCNHQRA+PKTF KSMENL +KID KK+  SEV   AK++KA++  SK +  K +YEKLKK+L   KE L K+  Q TDR++NKD+AL TSKLNYLDPRI+IAWC K NVP+ K+YNKTQ  KFQWA+ MTTE++KF D++SDK+
Sbjct:  129 KWTFLEHKGPMFAPPYEPLPSSVRFYYDGVQMRLSPEAEEVAGFYARMLDHEYTTKEIFNTNFFRDWQKTMTEEERRVIKRLDKCDFRPMFEYFKEKSEERKNMTKEEKQKIKEANQAISEEYGFCTLDGHKQRIANFKIEPPNLFRGRGDHPKMGMLKKRIMPEDIIINCSKDSKIPAPPPGHRWKEVRHDNTVTWLACWIENIQSNFKYIMLNASSRLKGEKDWKKYETARKLHQYVDKIRADYRTDFKSKEMRIRQRAVALYFIDKLALRAGNEKDEDEADTVGCCSLRCEHVTLHEELDGKPFVVEFDFLGKDSIRYQNSVPVPKRVFKNLKLFMKNKQGSDDLFDRLNTSILNQYLRELMEGLTAKVFRTYNASRTLEEQLELLTNPDDSVAAKLLSYNRANRAVAVLCNHQRAVPKTFAKSMENLQKKIDAKKEQISEVTREAKQIKADWKHSKQASQKVQYEKLKKRLATLKEQLLKLQVQATDRDENKDIALSTSKLNYLDPRITIAWCRKLNVPVEKVYNKTQRDKFQWAIQMTTEDFKFLDVSSDKN 685
[Close]
BLAST of DNA topoisomerase I vs. TrEMBL
Match: A0A074Z8H7 (DNA topoisomerase I OS=Opisthorchis viverrini OX=6198 GN=T265_11721 PE=3 SV=1)

HSP 1 Score: 747.273 bits (1928), Expect = 0.000e+0
Identity = 369/564 (65.43%), Postives = 449/564 (79.61%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKFQDLNSDKSTNVLK 2041
            G KW FLEH GP+F   YE LP +V+FYYDG+ ++L    EE+A FYA+M DHDYT  +VFNKNF +DW+ VM+ DE+ +IK LSKCDF+ M  +F KL+E++K   KEEK+K K +N  ++N+YGYC +D ++Q+IGNF+IEPPGLFRGRGNHPKMG +K+R++PE VIINCSKNSKIP PP G +WKEVRHD TVTWLACW ENVQNNFKYVMLN SSRLKGEKDWKKYETAR LH+ ID+IR DY  DF H+EMRIRQRAVALYFID+LALRAGNEK EDEADTVGCCSLR EHI L  +    +Y+V+FDFLGKDSIRYQN V+VPK+V+KN+  F + K+E DDLFDRLNT+ LNQYLRELM GLTAKVFRTYNAS TLE +L       D+   K+LAYN ANR VA+LCNHQRA+PK+F KSMENL +KID K+   +E+   AK++KA+Y ++K    K  YEKLKK+L   KE L K+  Q TDR++NK++AL TSKLNYLDPRI++AWC + NVP+ K+YNKTQ +KFQWA+DM T  YKF D +SD++TN LK
Sbjct:  465 GVKWTFLEHKGPIFAPPYERLPDNVRFYYDGQPVRLSDAAEEVAGFYARMLDHDYTTKEVFNKNFFKDWLKVMNDDERRIIKSLSKCDFREMHAHFLKLAEERKNRTKEEKQKLKEENLALLNEYGYCTLDGHRQRIGNFRIEPPGLFRGRGNHPKMGMLKKRVLPEHVIINCSKNSKIPVPPKGHRWKEVRHDNTVTWLACWTENVQNNFKYVMLNPSSRLKGEKDWKKYETARGLHKIIDRIRADYRADFKHKEMRIRQRAVALYFIDRLALRAGNEKDEDEADTVGCCSLRYEHIKLHEELDGKQYVVEFDFLGKDSIRYQNSVAVPKRVFKNVRLFLQNKKENDDLFDRLNTTVLNQYLRELMEGLTAKVFRTYNASRTLEEQLELLTDPNDSPQEKLLAYNRANRAVAVLCNHQRAVPKSFAKSMENLQKKIDAKRDQIAEITSEAKQIKADYKRNKQVAKKVAYEKLKKRLSVAKEQLAKLQLQATDRDENKEIALSTSKLNYLDPRITVAWCKRFNVPIEKVYNKTQREKFQWAIDMATAEYKFLDRDSDRNTNKLK 1028
[Close]
BLAST of DNA topoisomerase I vs. TrEMBL
Match: A0A430QRQ7 (DNA topoisomerase I OS=Schistosoma bovis OX=6184 GN=DC041_0012350 PE=3 SV=1)

HSP 1 Score: 746.117 bits (1925), Expect = 0.000e+0
Identity = 375/564 (66.49%), Postives = 450/564 (79.79%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKFQDLNSDKSTNVLK 2041
            G KW+FLEH GP+F   YE LPS V F+YD K +KL    EE+A FYA+M DHDYT  +VFN NF RDW+  M+++E+  IK LSKCDF+ M  YF KLSE++K   KEEK+K K +N +I N+YGYC++D +KQ+IGNF+IEPPGLFRGRGNHPKMG +K+RI+PEDVIINCSK+SK+P PP G KWKEVRHD +VTWLACW EN+QN+FKY+MLN SSRLKGEKDWKKYETAR LH+ IDKIR DY  DF H+EMRIRQRAVALYFIDKLALRAGNEK EDEADTVGCCSLR EHI L  + +   Y+V+FDFLGKDSIRYQN VSVPK+V+KN+  F + K+E DDLFDRLNTS LNQYLRELM GLTAKVFRTYNAS TLE +L       D    K+LAYN ANR VA+LCNHQR++PK+F KSMENL +KID K++  SE+   AK++KA+Y   K +  K  YEKLKK+L   KE L+K+  Q TDR++NK++AL TSKLNYLDPRIS+AWC K NVP+ KI+N+TQ +KFQWA+DM T +YKF D++SD+STN LK
Sbjct:  125 GVKWKFLEHKGPLFAPPYERLPSSVHFHYDSKPIKLSDHAEEVAGFYARMLDHDYTTKEVFNHNFFRDWVKTMNEEERRTIKSLSKCDFREMHQYFLKLSEERKNRTKEEKQKIKEENLQIQNEYGYCILDGHKQRIGNFRIEPPGLFRGRGNHPKMGMLKKRIMPEDVIINCSKDSKVPAPPKGHKWKEVRHDNSVTWLACWTENIQNSFKYIMLNPSSRLKGEKDWKKYETARCLHKVIDKIRADYRADFKHKEMRIRQRAVALYFIDKLALRAGNEKDEDEADTVGCCSLRYEHIKLHEELNGQPYVVEFDFLGKDSIRYQNAVSVPKRVFKNVKLFLKNKKENDDLFDRLNTSVLNQYLRELMDGLTAKVFRTYNASRTLEEQLERLTNPDDPPHAKLLAYNRANRAVAVLCNHQRSVPKSFAKSMENLQKKIDAKREQISEITSEAKQIKADYKNHKQASKKVAYEKLKKRLASAKEALSKLQLQATDRDENKEIALSTSKLNYLDPRISVAWCKKYNVPIEKIFNRTQREKFQWAIDMATCDYKFLDVDSDRSTNKLK 688
[Close]
BLAST of DNA topoisomerase I vs. TrEMBL
Match: A0A4S2LVU2 (DNA topoisomerase I OS=Opisthorchis felineus OX=147828 GN=CRM22_004515 PE=3 SV=1)

HSP 1 Score: 746.117 bits (1925), Expect = 0.000e+0
Identity = 371/564 (65.78%), Postives = 449/564 (79.61%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKFQDLNSDKSTNVLK 2041
            G KW FLEH GP+F   YE LP +V+FYYDG+ ++L    EE+A FYA+M DHDYT  +VFNKNF +DW+ VM+ DE+ +IK LSKCDF+ M  YF KL+E++K   KEEK+K K +N  ++N+YGYC +D ++Q+IGNF+IEPPGLFRGRGNHPKMG +K+R++PE VIINCSKNSKIP PP G +WKEVRHD TVTWLACW ENVQNNFKYVMLN SSRLKGEKDWKKYETAR LH+ ID+IR DY  DF H+EMRIRQRAVALYFIDKLALRAGNEK EDEADTVGCCSLR EHI L  +    +Y+V+FDFLGKDSIRYQN V+VPK+V+KN+  F + K+E DDLFDRLNT+ LNQYLRELM GLTAKVFRTYNAS TLE +L       D+   K+LAYN ANR VA+LCNHQRA+PK+F KSMENL +KID K+   +E+   AK++KA+Y ++K    K  YEKLKK+L   KE L K+  Q TDR++NK++AL TSKLNYLDPRI++AWC + NVP+ K+YNKTQ +KFQWA+DM T  YKF D +SD++TN LK
Sbjct:  134 GVKWTFLEHKGPIFAPPYERLPDNVRFYYDGQPVRLSDAAEEVAGFYARMLDHDYTTKEVFNKNFFKDWLKVMNDDERRIIKSLSKCDFREMHAYFLKLTEERKNRTKEEKQKLKEENLALLNEYGYCTLDGHRQRIGNFRIEPPGLFRGRGNHPKMGMLKKRVLPEHVIINCSKNSKIPVPPKGHRWKEVRHDNTVTWLACWTENVQNNFKYVMLNPSSRLKGEKDWKKYETARGLHKIIDRIRADYRADFKHKEMRIRQRAVALYFIDKLALRAGNEKDEDEADTVGCCSLRYEHIKLHEELDGKQYVVEFDFLGKDSIRYQNSVAVPKRVFKNVRLFLQNKKENDDLFDRLNTTVLNQYLRELMEGLTAKVFRTYNASRTLEEQLELLTDPNDSPHEKLLAYNRANRAVAVLCNHQRAVPKSFAKSMENLQKKIDAKRDQIAEITSEAKQIKADYKRNKQVAKKVAYEKLKKRLSVAKEQLAKLQLQATDRDENKEIALSTSKLNYLDPRITVAWCKRFNVPIEKVYNKTQREKFQWAIDMATAEYKFLDRDSDRNTNKLK 697
[Close]
BLAST of DNA topoisomerase I vs. TrEMBL
Match: H2KNI9 (DNA topoisomerase I OS=Clonorchis sinensis OX=79923 GN=CLF_100246 PE=3 SV=1)

HSP 1 Score: 743.806 bits (1919), Expect = 0.000e+0
Identity = 369/564 (65.43%), Postives = 450/564 (79.79%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKFQDLNSDKSTNVLK 2041
            G KW FLEH GP+F   YE LP +V+FYYDG+ ++L    EE+A FYA+M DHDYT  +VFNKNF +DW+ VM+ DE+ +IK LSKCDF+ M  +F KL+E++K   KEEK+K K +N  ++N+YGYC +D ++Q+IGNF+IEPPGLFRGRGNHPKMG +K+R++PE VIINCSKNSKIP PP G +WKEVRHD TVTWLACW ENVQNNFKYVMLN SSRLKGEKDWKKYETAR LH+ ID+IR DY  DF H+EMRIRQRAVALYFID+LALRAGNEK EDEADTVGCCSLR EHI L  +    +Y+V+FDFLGKDSIRYQN V+VPK+V+KN+  F + K+E DDLFDRLNT+ LNQYLRELM GLTAKVFRTYNAS TLE +L       D+   K+LAYN ANR VA+LCNHQRA+PK+F KSMENL +KID K+   +E+ + AK++KA+Y ++K    K  YEKLKK+L   KE L K+  Q TDR++NK++AL TSKLNYLDPRI++AWC + NVP+ K+YNKTQ +KFQWA+DM T  YKF D +SD++TN LK
Sbjct:  134 GVKWTFLEHKGPIFAPPYERLPDNVRFYYDGQPVRLSDAAEEVAGFYARMLDHDYTTKEVFNKNFFKDWLKVMNDDERRIIKSLSKCDFREMHAHFLKLTEERKNRTKEEKQKLKEENLALLNEYGYCTLDGHRQRIGNFRIEPPGLFRGRGNHPKMGMLKKRVLPEHVIINCSKNSKIPVPPKGHRWKEVRHDNTVTWLACWTENVQNNFKYVMLNPSSRLKGEKDWKKYETARGLHKIIDRIRADYRADFKHKEMRIRQRAVALYFIDRLALRAGNEKDEDEADTVGCCSLRYEHIKLHEELDGKQYVVEFDFLGKDSIRYQNSVAVPKRVFKNVRLFLQNKKENDDLFDRLNTTVLNQYLRELMEGLTAKVFRTYNASRTLEEQLELLTDPNDSPQEKLLAYNRANRAVAVLCNHQRAVPKSFAKSMENLQKKIDAKRDQIAEITNEAKQIKADYKRNKQVAKKVAYEKLKKRLSVAKEQLAKLQLQATDRDENKEIALSTSKLNYLDPRITVAWCKRFNVPIEKVYNKTQREKFQWAIDMATAEYKFLDRDSDRNTNKLK 697
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Cavefish
Match: zgc:173742 (DNA topoisomerase I, mitochondrial-like [Source:NCBI gene;Acc:103027608])

HSP 1 Score: 652.899 bits (1683), Expect = 0.000e+0
Identity = 331/552 (59.96%), Postives = 410/552 (74.28%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP F   Y+PLP +V+FYYDGK +KL  + EE+A F+ +M DH+YT  +VF  NF +DW   M+ +E+ +I DL+KCDF  +    K+  E +K + KEEK   K  N++I+ +YG+CM+D ++++IGNFKIEPP LFRGRG HPK G +K+RI PEDVIINCSK+S IP+PPAG KWKEVRHD TVTWLA W ENVQ + KYVMLNA+S+LKGEKDW+KYE ARKL   +D IR  Y  D   ++M  RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHITL      ++ +V+FDFLGKD IRY N+V V K+V+KNL  F E KQEGDDLFDRLNT+TLN++L  LMPGLTAKVFRTYNASITL+++L      +DNV  K+L+YN ANR VA+LCNHQRA PKTFE+SM NL  KID +K+     K   K+ K E  +S D+ +    EK KK +++ +E L KM  Q TDRE+NK +AL TSKLNYLDPRIS+AWC    VPL+KIYNK+   KF WA+DMT  +++F
Sbjct:  193 GVKWKFLEHKGPYFPPEYQPLPDNVRFYYDGKAVKLSLQAEEVALFFGQMLDHEYTTKEVFRNNFFKDWRKEMTLEERMLITDLNKCDFGELHTMHKERVEARKNMSKEEKLVIKEANQKIVEEYGFCMLDHHRERIGNFKIEPPALFRGRGEHPKQGMLKKRIQPEDVIINCSKDSCIPQPPAGHKWKEVRHDNTVTWLASWTENVQGSCKYVMLNANSKLKGEKDWEKYEVARKLKTCVDAIRSQYQEDLKSKQMGTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHDTIDGEKCVVEFDFLGKDCIRYYNKVPVSKRVFKNLKLFMENKQEGDDLFDRLNTATLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNPSDNVPEKLLSYNRANRAVAVLCNHQRAPPKTFEQSMANLQAKIDSRKEQLVLAKKELKQAKKEAKESSDNKLLVLVEKKKKAVQRCEEQLLKMEVQATDREENKQIALSTSKLNYLDPRISVAWCKNLEVPLDKIYNKSLRDKFAWAIDMTEMDFQF 744
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Cavefish
Match: top1 (DNA topoisomerase I [Source:ZFIN;Acc:ZDB-GENE-030131-5390])

HSP 1 Score: 634.41 bits (1635), Expect = 0.000e+0
Identity = 324/552 (58.70%), Postives = 406/552 (73.55%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLP +V+F+YDGK MKL    EE+ATF+AKM DH+YT  D+F KNF +DW   M+ +EKS I DL+KC+F+ M  YFK  +E +K + KEEK K K +NE I+ +YG+C+MD++K++IGNF+IEPPGLFRGRG+HPKMG +KRRI PED+IINCSK+SK PKPP G +WKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +++IR  Y  D+  +EMRIRQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  +    +Y+V+FDFLGKDSIRY N++ V K+V+KNL  F E KQ  DDLFDRLNTS LN++L+ELM GLTAKVFRTYNASITL+++L       +N   K+L+YN ANR V +            +KSM+NL  KID KK+  S  K   K  K +     D   ++  E  KK + +  + L K+  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC K  V + KIYNKTQ +KF WA+DM  E+++F
Sbjct:   24 GSKWRFLEHKGPVFAPPYEPLPDNVRFFYDGKAMKLSESAEEVATFFAKMLDHEYTTKDIFRKNFFKDWRKEMTSEEKSKITDLNKCNFREMSEYFKAQTEARKQMTKEEKLKIKEENERIVQEYGFCLMDNHKERIGNFRIEPPGLFRGRGDHPKMGMLKRRIRPEDIIINCSKDSKHPKPPPGTRWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARQLKKCVERIRAQYHEDWKSKEMRIRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLYPELDGQDYVVEFDFLGKDSIRYYNKIPVEKRVFKNLQLFMESKQPEDDLFDRLNTSILNKHLQELMEGLTAKVFRTYNASITLQQQLKQLTSPEENAPAKILSYNRANRAVNLT-----------KKSMQNLQTKIDAKKQQLSAAKKELKSAKVDAKTMHDEKSRKALETKKKAVARLSDQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKWGVGIEKIYNKTQREKFAWAIDMADEDFEF 564
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Cavefish
Match: ENSAMXT00000022486.2 (DNA topoisomerase 1-like [Source:NCBI gene;Acc:103043264])

HSP 1 Score: 633.254 bits (1632), Expect = 0.000e+0
Identity = 333/555 (60.00%), Postives = 415/555 (74.77%), Query Frame = 2 
Query:  344 AGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADN--VSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            + G KW  LEH GP+F   YEPLPS+V F YDG+ +KL   TEE+ATFYAKM DH+YT  ++F  NF  DW  VM++ E+ +IK LSKCDF  +  YF + +E+KK + KEEK+  K +  ++   YGYC++D +K+KIGNF++EPPGLFRGRG HPKMG +K+R+ PEDVIINCS+++KIP PP G +WK V+HD +VTWLA W EN+  + KY+MLN SS+LKGEKDW+KYETARKL   +D IR +Y   ++ REM+ RQR VALYFIDKLALRAGNEK +D ADTVGCCSLRVEHITL       EY+V+FDFLGKDSIRY N+V V K+V+KNL  F E K   DDLFDR++T  LN+ L E MPGLTAKVFRT+NAS TL+ +L  KK+  D+  V  K+LAYN ANR VAILCNHQRA PKTFEKSM+ L EKI KKK++  + K   K+ K E+ +S     K+  EK +K L++  E L K+  Q+TDRE+NK +ALGTSKLNYLDPRIS+AWC KNNVP+ KIYNKT   KF WA+DMT EN++F
Sbjct:   44 SDGQKWSTLEHNGPLFPPEYEPLPSNVPFLYDGEPVKLSLATEEVATFYAKMLDHEYTTKEIFQNNFFTDWREVMTESERRLIKKLSKCDFSSIHKYFVEKAEEKKNMTKEEKQVLKDEANKLTEHYGYCLLDGHKEKIGNFRVEPPGLFRGRGEHPKMGKLKKRVQPEDVIINCSQDAKIPDPPVGHRWKRVQHDNSVTWLASWVENIHAHIKYIMLNPSSKLKGEKDWQKYETARKLKLSVDSIRSEYRKAWTAREMKQRQRGVALYFIDKLALRAGNEKDDDTADTVGCCSLRVEHITLHRRLDDQEYVVEFDFLGKDSIRYYNKVPVEKQVFKNLKLFMESKNPEDDLFDRISTIYLNKKLNEAMPGLTAKVFRTFNASTTLQEQL--KKLTTDDMTVEEKILAYNRANRAVAILCNHQRAAPKTFEKSMQLLQEKIKKKKEEVEKAKKELKQAKKEHKESPSEKSKKLVEKKEKTLKRLLEQLKKLQLQETDREENKVIALGTSKLNYLDPRISVAWCKKNNVPVEKIYNKTHRAKFAWAIDMTDENFQF 596
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Cavefish
Match: zgc:173742 (DNA topoisomerase I, mitochondrial-like [Source:NCBI gene;Acc:103027608])

HSP 1 Score: 617.076 bits (1590), Expect = 0.000e+0
Identity = 314/519 (60.50%), Postives = 387/519 (74.57%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAW 1903
            G KW+FLEH GP F   Y+PLP +V+FYYDGK +KL  + EE+A F+ +M DH+YT  +VF  NF +DW   M+ +E+ +I DL+KCDF  +    K+  E +K + KEEK   K  N++I+ +YG+CM+D ++++IGNFKIEPP LFRGRG HPK G +K+RI PEDVIINCSK+S IP+PPAG KWKEVRHD TVTWLA W ENVQ + KYVMLNA+S+LKGEKDW+KYE ARKL   +D IR  Y  D   ++M  RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHITL      ++ +V+FDFLGKD IRY N+V V K+V+KNL  F E KQEGDDLFDRLNT+TLN++L  LMPGLTAKVFRTYNASITL+++L      +DNV  K+L+YN ANR VA+LCNHQRA PKTFE+SM NL  KID +K+     K   K+ K E  +S D+ +    EK KK +++ +E L KM  Q TDRE+NK +AL TSKLNYLDPRIS+AW
Sbjct:  193 GVKWKFLEHKGPYFPPEYQPLPDNVRFYYDGKAVKLSLQAEEVALFFGQMLDHEYTTKEVFRNNFFKDWRKEMTLEERMLITDLNKCDFGELHTMHKERVEARKNMSKEEKLVIKEANQKIVEEYGFCMLDHHRERIGNFKIEPPALFRGRGEHPKQGMLKKRIQPEDVIINCSKDSCIPQPPAGHKWKEVRHDNTVTWLASWTENVQGSCKYVMLNANSKLKGEKDWEKYEVARKLKTCVDAIRSQYQEDLKSKQMGTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHDTIDGEKCVVEFDFLGKDCIRYYNKVPVSKRVFKNLKLFMENKQEGDDLFDRLNTATLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNPSDNVPEKLLSYNRANRAVAVLCNHQRAPPKTFEQSMANLQAKIDSRKEQLVLAKKELKQAKKEAKESSDNKLLVLVEKKKKAVQRCEEQLLKMEVQATDREENKQIALSTSKLNYLDPRISVAW 711
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Sea Lamprey
Match: top1 (DNA topoisomerase I [Source:ZFIN;Acc:ZDB-GENE-030131-5390])

HSP 1 Score: 671.003 bits (1730), Expect = 0.000e+0
Identity = 342/552 (61.96%), Postives = 418/552 (75.72%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G +W+FLEH GP+F   YEPLP  V FYY+GK M+L  + EE+ATFYAKM DH+YT  D+F KNF +DW   M+ +E+SVI DLSKC+F  +  YFK  +E++K + K+EK+  K KNE++   +G+C+MDS+K++IGNF+IEPPGLFRGRG+HPK G +KRR++P+DVIINCSK+SKIP PP G KWKEVRHD  VTWLA W ENVQ   KYVMLNA+SR+KGEKDW+KYE ARKL   ++KIR  Y +D+  +EMRIRQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L       EY+V+FDF GKDSIRY N+V V K+V+KNL  F E KQ GDDLFDRLNTS LN++L+ELM GLTAKVFRTYNASITL+ +L       ++V  K+L+YN ANR VAILCNHQRA PKTFEKSM NL  KID K++  +  K   K  K +    KD   K+  E  KK +E+ +E L K+    TD+E+NK +ALGTSKLNYLDPRIS+AWC K  VPL KIYNKTQ +KF WAVDM  ++++F
Sbjct:  211 GVRWKFLEHQGPVFAPPYEPLPDDVHFYYNGKPMRLSEKAEEVATFYAKMLDHEYTSKDIFRKNFFKDWRKEMTSEERSVITDLSKCNFNEIDEYFKVKTEERKNMTKDEKQMLKEKNEKMTQDFGFCLMDSHKERIGNFRIEPPGLFRGRGDHPKQGMLKRRVLPQDVIINCSKDSKIPVPPPGHKWKEVRHDNKVTWLASWTENVQGQVKYVMLNATSRIKGEKDWQKYEVARKLKTCVEKIRAQYRDDWKSKEMRIRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLHEKMDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQGGDDLFDRLNTSILNKHLQELMDGLTAKVFRTYNASITLQWQLKELTSTDESVPAKILSYNRANRAVAILCNHQRAAPKTFEKSMANLQGKIDGKREQLALAKRELKSAKTDLKVRKDIKSKKVVETRKKAVERMEEQLMKLEVTATDKEENKQIALGTSKLNYLDPRISVAWCKKFGVPLEKIYNKTQREKFAWAVDMAEKDFEF 762
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Yeast
Match: TOP1 (Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation [Source:SGD;Acc:S000005366])

HSP 1 Score: 449.129 bits (1154), Expect = 9.686e-147
Identity = 231/443 (52.14%), Postives = 307/443 (69.30%), Query Frame = 2 
Query:  356 KWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSV----IKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKE-GKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLEREL---VNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKI 1660
            KW  L+H G +F   Y+PLPSH+K YYDGK + L P+ EE+A F+A + + D+ KN VF KNF  D++ V+ +    +    IK+ S+CDF  M +YF+   E KK L  +EK++ +L+ E+    Y +C +D  ++++GNFK+EPP LFRGRG HPK G +KRR+ PED+++N SK++ +P  P G KW E+RHD TV WLA W+EN+ N+FKYV L A+S LKG+ D+KK+E AR+L   ID IRRDYT +   + M  RQ+AVA+Y ID  ALRAG EK EDEADTVGCCSLR EH+TL+  ++     V FDFLGKDSIR+  EV V K+V+KNL  FK   KQ G  LFDRL+ S LN+YL+  MPGLTAKVFRTYNAS T++ +L    NK  VA+    K+L YN+ANR VAILCNHQR + K   +++E  + +I
Sbjct:  144 KWVTLKHNGVIFPPPYQPLPSHIKLYYDGKPVDLPPQAEEVAGFFAALLESDHAKNPVFQKNFFNDFLQVLKESGGPLNGIEIKEFSRCDFTKMFDYFQLQKEQKKQLTSQEKKQIRLEREKFEEDYKFCELDGRREQVGNFKVEPPDLFRGRGAHPKTGKLKRRVNPEDIVLNLSKDAPVPPAPEGHKWGEIRHDNTVQWLAMWRENIFNSFKYVRLAANSSLKGQSDYKKFEKARQLKSYIDAIRRDYTRNLKSKVMLERQKAVAIYLIDVFALRAGGEKSEDEADTVGCCSLRYEHVTLKPPNT-----VIFDFLGKDSIRFYQEVEVDKQVFKNLTIFKRPPKQPGHQLFDRLDPSILNKYLQNYMPGLTAKVFRTYNASKTMQDQLDLIPNKGSVAE----KILKYNAANRTVAILCNHQRTVTKGHAQTVEKANNRI 577

HSP 2 Score: 76.2554 bits (186), Expect = 9.675e-15
Identity = 31/62 (50.00%), Postives = 48/62 (77.42%), Query Frame = 2 
Query: 1817 QQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            Q  D+E+N  V+LGTSK+NY+DPR+S+ +C K +VP+ KI+ KT  +KF+WA++   EN++F
Sbjct:  708 QLKDKEENSQVSLGTSKINYIDPRLSVVFCKKYDVPIEKIFTKTLREKFKWAIESVDENWRF 769
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Nematostella
Match: EDO35433 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SLG4])

HSP 1 Score: 585.874 bits (1509), Expect = 0.000e+0
Identity = 301/502 (59.96%), Postives = 383/502 (76.29%), Query Frame = 2 
Query:  497 MFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            M +HDYT  ++FNKNF  DW   M+++E+  IKDL  CDFK M  YF+  +E++K + KEEK + K KNEEI+N+YG+CMMD +KQ++GNFKIEPPGLFRGRG+HPK G +K+R++PE+V IN  K++K+P PPAG KWK+V  D TVTWLACW EN+Q ++KYVMLN +SRLKGEKDW+KYETARKL E +DKIR DY  D+  +EMR+RQR VALYFIDKLALRAG+EK +D ADTVGCCSLRVEHI L  +    E +V+FDFLGKDS+RY+N V + K+ +KN+  F EGKQEGDDLFDRL T+++N+YL ELM GL+AKVFRT+NAS+TL+ +L       DNV+ K+L+YN ANR VAILCNHQR  PKTFEKSM NL  KI  KKK   E K   K L+ +  +SK     +  EK K ++++ +E L K+    TD+E+NK++ALGTSKLNYLDPRI++AWC K  VP+ K YNKTQ +KF WA+DM  E++ F
Sbjct:    1 MLEHDYTSKEIFNKNFFSDWRKEMTEEERKHIKDLKNCDFKEMHKYFEMKNEERKNMTKEEKLEIKKKNEEILNEYGFCMMDHHKQRVGNFKIEPPGLFRGRGDHPKQGKLKKRVMPEEVTINIGKDAKVPDPPAGHKWKKVICDDTVTWLACWIENIQGSYKYVMLNPTSRLKGEKDWRKYETARKLKECVDKIREDYRKDWKSKEMRLRQRGVALYFIDKLALRAGHEKDDDTADTVGCCSLRVEHIKLHEEWEDKECVVEFDFLGKDSVRYRNFVPIEKRAFKNMKLFMEGKQEGDDLFDRLTTTSVNKYLSELMEGLSAKVFRTFNASLTLQDQLQKLTNPEDNVNAKILSYNRANRAVAILCNHQRTAPKTFEKSMSNLQLKISDKKKTVKEAKKELKALRVKAKESKSESNNKAVEKKKAQVQRLEEQLFKLEVTATDKEENKEIALGTSKLNYLDPRITVAWCKKFEVPIEKAYNKTQREKFAWAIDMAGEDFVF 502
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Medaka
Match: top1l (DNA topoisomerase I [Source:NCBI gene;Acc:101164052])

HSP 1 Score: 691.804 bits (1784), Expect = 0.000e+0
Identity = 347/552 (62.86%), Postives = 426/552 (77.17%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLPS VKFYYDGK M+L PE EE+ATF+AKM DH+YT  D+F KNF +DW   M+ +EK+ I DL KC+F  M  YFK  SE +KA+ KEEK+K K +NE ++ +YGYC+MD++K++I NF+IEPPGLFRGRG+HPKMG +KRRI PED+IINCSK+SK P+PP G KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +D+IR  Y  D+  +EMR+RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  ++   EY+V+FDFLGKDSIRY N+V V K+V+KNL  F E K+  DDLFDRLNTS LN++L+ELM GLTAKVFRTYNASITL+++L       +N+  K+L+YN ANR VAILCNHQRA PKTFEKSM+NL  KID KK   S+ K   K  KA+    KD   K+  E  +K +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC K  +P+ KIYNKTQ +KF WA+DM  E+Y+F
Sbjct:  199 GVKWKFLEHKGPVFAPPYEPLPSRVKFYYDGKPMRLSPEAEEVATFFAKMLDHEYTTKDIFRKNFFKDWRKEMTAEEKATITDLKKCNFTEMSEYFKAQSEARKAMSKEEKQKIKEENERLLQKYGYCIMDNHKERIANFRIEPPGLFRGRGDHPKMGMLKRRIRPEDIIINCSKDSKCPEPPPGTKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDRIRAQYREDWKSKEMRVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLYPENEGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKEPDDDLFDRLNTSILNKHLQELMDGLTAKVFRTYNASITLQQQLKELTTADENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMQNLQAKIDAKKDQLSDAKRELKSAKADAKVRKDEKTKKAVESKRKAVQRIEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKWGIPVEKIYNKTQREKFAWAIDMADEDYEF 750
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Medaka
Match: top1l (DNA topoisomerase I [Source:NCBI gene;Acc:101164052])

HSP 1 Score: 690.263 bits (1780), Expect = 0.000e+0
Identity = 347/552 (62.86%), Postives = 426/552 (77.17%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F   YEPLPS VKFYYDGK M+L PE EE+ATF+AKM DH+YT  D+F KNF +DW   M+ +EK+ I DL KC+F  M  YFK  SE +KA+ KEEK+K K +NE ++ +YGYC+MD++K++I NF+IEPPGLFRGRG+HPKMG +KRRI PED+IINCSK+SK P+PP G KWKEVRHD  VTWL  W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +D+IR  Y  D+  +EMR+RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L  ++   EY+V+FDFLGKDSIRY N+V V K+V+KNL  F E K+  DDLFDRLNTS LN++L+ELM GLTAKVFRTYNASITL+++L       +N+  K+L+YN ANR VAILCNHQRA PKTFEKSM+NL  KID KK   S+ K   K  KA+    KD   K+  E  +K +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC K  +P+ KIYNKTQ +KF WA+DM  E+Y+F
Sbjct:   26 GVKWKFLEHKGPVFAPPYEPLPSRVKFYYDGKPMRLSPEAEEVATFFAKMLDHEYTTKDIFRKNFFKDWRKEMTAEEKATITDLKKCNFTEMSEYFKAQSEARKAMSKEEKQKIKEENERLLQKYGYCIMDNHKERIANFRIEPPGLFRGRGDHPKMGMLKRRIRPEDIIINCSKDSKCPEPPPGTKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDRIRAQYREDWKSKEMRVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLYPENEGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKEPDDDLFDRLNTSILNKHLQELMDGLTAKVFRTYNASITLQQQLKELTTADENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMQNLQAKIDAKKDQLSDAKRELKSAKADAKVRKDEKTKKAVESKRKAVQRIEEQLMKLEVQATDREENKQIALGTSKLNYLDPRISVAWCKKWGIPVEKIYNKTQREKFAWAIDMADEDYEF 577
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Medaka
Match: top1 (DNA topoisomerase I [Source:ZFIN;Acc:ZDB-GENE-030131-5390])

HSP 1 Score: 686.411 bits (1770), Expect = 0.000e+0
Identity = 347/552 (62.86%), Postives = 425/552 (76.99%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+FLEH GP+F  AYEPLP  VKFYY+GK MKL    EE+ATF+AKM DH+YT  D+F KNF +DW   M+ +EKS+I DL KCDF  M  YFK  SE +K + KEEK+K K +NE ++++YG+C+MD+++++IGNF+IEPPGLFRGRG+HPKMG +KRRI PEDVIINCSK+SK PKPP G KWKEVRHD  VTWLA W EN+Q + KY+MLN SSR+KGEKDW+KYETAR+L + +D+IR  Y  D+  +EMRIRQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHI L       EY+V+FDFLGKDSIRY N++ V K+V+KNL  F E KQ  DDLFDRLNTS LN++L+ELM GLTAKVFRTYNASITL+++L       D++  K+L+YN ANR VAILCNHQRA PKTFEKSM+NL  KID+K+   S  +   K  KA +  S D   K+  E  +K +++ +E L K+  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC K  VP+ KIYNKTQ +KF WA+DM  ++Y+F
Sbjct:   36 GSKWRFLEHKGPVFVPAYEPLPEKVKFYYNGKPMKLSAPAEEVATFFAKMLDHEYTTKDIFRKNFYKDWRKEMTSEEKSIITDLKKCDFSEMNEYFKAQSEARKQMSKEEKQKIKEENERLLSEYGFCIMDNHRERIGNFRIEPPGLFRGRGDHPKMGMLKRRIRPEDVIINCSKDSKHPKPPPGTKWKEVRHDNKVTWLASWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDRIRTQYREDWKSKEMRIRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHIKLFPKMDEQEYVVEFDFLGKDSIRYYNKIPVEKRVFKNLQLFLENKQPEDDLFDRLNTSILNKHLQELMDGLTAKVFRTYNASITLQQQLKELTCCDDSIPAKLLSYNRANRAVAILCNHQRAPPKTFEKSMQNLQTKIDEKQNQLSAARKQLKAAKANHKASHDDKSKKAVEVKRKAVQRIEEQLMKLQMQATDREENKQIALGTSKLNYLDPRISVAWCKKWGVPIEKIYNKTQREKFAWAIDMAEKDYEF 587
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Medaka
Match: zgc:173742 (DNA topoisomerase I, mitochondrial [Source:NCBI gene;Acc:101159011])

HSP 1 Score: 652.514 bits (1682), Expect = 0.000e+0
Identity = 331/554 (59.75%), Postives = 408/554 (73.65%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXX--XMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+ LEH GP F   Y+PLP  V FYYDGK MKL    EE+A F+A+M DH+YT   VF +NF +DW   M+ +E+SVI+DL+KCDF  +    K   E +K + KEEK+  K  N++I++QYGYC++D +K++IGNFKIEPPGLFRGRG HPK G +K+RI PEDVIINCSK+++IP PP G  WKEVRHD TVTWLA W EN+Q + KY+MLNA+S+LKGEKDW+KYE ARKL   +D IR  Y  D   REM  RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHITL      ++ +V+FDFLGKDSIRY N+V V  KV+K+L RF + K+ GD+LFDRLNTS LN++L  LMPGLTAKVFRTYNASITL+++L      +DNV+ K+L+YN ANR VAILCNHQRA PKTFE+SM NL  KID +K+  +  K   K+ K E     +S IK +    +KK    +   +  KM  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC    VP+ KIYNKTQ +KF WA+DMT ++++F
Sbjct:  186 GVKWKSLEHNGPYFPPEYQPLPDDVHFYYDGKKMKLSVAAEEVALFFAQMLDHEYTTKKVFRENFFKDWRKEMTPEERSVIQDLNKCDFGEIHAMHKTKVEARKNMTKEEKQTLKEANQKIVDQYGYCLLDHHKERIGNFKIEPPGLFRGRGEHPKQGMLKKRIQPEDVIINCSKDARIPDPPPGHHWKEVRHDNTVTWLASWTENIQGSIKYIMLNANSKLKGEKDWEKYEIARKLKSCVDDIRNTYLGDLKSREMVTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHEQLDGNKCVVEFDFLGKDSIRYYNKVPVISKVFKSLKRFMDNKEPGDELFDRLNTSLLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNKSDNVAEKLLSYNRANRAVAILCNHQRAPPKTFEQSMANLQAKIDARKEQLALAKSELKQAKKEAKDKGNSDIKLQALVERKKAAVKRCEEHLLKMEVQATDREENKQIALGTSKLNYLDPRISVAWCKNMEVPIEKIYNKTQREKFAWAIDMTEDDFEF 739
[Close]
BLAST of DNA topoisomerase I vs. Ensembl Medaka
Match: zgc:173742 (DNA topoisomerase I, mitochondrial [Source:NCBI gene;Acc:101159011])

HSP 1 Score: 652.129 bits (1681), Expect = 0.000e+0
Identity = 331/554 (59.75%), Postives = 408/554 (73.65%), Query Frame = 2 
Query:  350 GDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDE-ADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXX--XMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKF 2002
            G KW+ LEH GP F   Y+PLP  V FYYDGK MKL    EE+A F+A+M DH+YT   VF +NF +DW   M+ +E+SVI+DL+KCDF  +    K   E +K + KEEK+  K  N++I++QYGYC++D +K++IGNFKIEPPGLFRGRG HPK G +K+RI PEDVIINCSK+++IP PP G  WKEVRHD TVTWLA W EN+Q + KY+MLNA+S+LKGEKDW+KYE ARKL   +D IR  Y  D   REM  RQRAVALYFIDKLALRAGNEK E E ADTVGCCSLRVEHITL      ++ +V+FDFLGKDSIRY N+V V  KV+K+L RF + K+ GD+LFDRLNTS LN++L  LMPGLTAKVFRTYNASITL+++L      +DNV+ K+L+YN ANR VAILCNHQRA PKTFE+SM NL  KID +K+  +  K   K+ K E     +S IK +    +KK    +   +  KM  Q TDRE+NK +ALGTSKLNYLDPRIS+AWC    VP+ KIYNKTQ +KF WA+DMT ++++F
Sbjct:  153 GVKWKSLEHNGPYFPPEYQPLPDDVHFYYDGKKMKLSVAAEEVALFFAQMLDHEYTTKKVFRENFFKDWRKEMTPEERSVIQDLNKCDFGEIHAMHKTKVEARKNMTKEEKQTLKEANQKIVDQYGYCLLDHHKERIGNFKIEPPGLFRGRGEHPKQGMLKKRIQPEDVIINCSKDARIPDPPPGHHWKEVRHDNTVTWLASWTENIQGSIKYIMLNANSKLKGEKDWEKYEIARKLKSCVDDIRNTYLGDLKSREMVTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHEQLDGNKCVVEFDFLGKDSIRYYNKVPVISKVFKSLKRFMDNKEPGDELFDRLNTSLLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNKSDNVAEKLLSYNRANRAVAILCNHQRAPPKTFEQSMANLQAKIDARKEQLALAKSELKQAKKEAKDKGNSDIKLQALVERKKAAVKRCEEHLLKMEVQATDREENKQIALGTSKLNYLDPRISVAWCKNMEVPIEKIYNKTQREKFAWAIDMTEDDFEF 706
[Close]
BLAST of DNA topoisomerase I vs. Planmine SMEST
Match: SMESG000055403.1 (SMESG000055403.1)

HSP 1 Score: 1186.4 bits (3068), Expect = 0.000e+0
Identity = 669/671 (99.70%), Postives = 671/671 (100.00%), Query Frame = 2 
Query:   29 MSKIAINGNGATIKLKKEIVSDDDDVPLSKRVTNEANVSEDDMPLFNNRKRISKASDRXXXXXXXXXXXXXXXXXXXISEIKSPXXXXXXXXXXXXXXXXXXXXRAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSXXXXXXXXXXXXXXXXXXXXIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIXXXXXXXXXXXXXXXXXXXXMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKFQDLNSDKSTNVLK 2041
            MSKIAINGNGATIKLKKEIVSDDDDVPLSKRVTNEANVS+DDMPLFNNRKRISKASDRSSQPEKKKVKKEIKSPKNSISEIKSPKKGKVKKEEETVWEWWKEEKRAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSEDKKALPKEEKEKNKLKNEEIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRA+PKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIKEKYEKLKKKLEKTKEMLNKMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKFQDLNSDKSTNVLK
Sbjct:    1 MSKIAINGNGATIKLKKEIVSDDDDVPLSKRVTNEANVSDDDMPLFNNRKRISKASDRSSQPEKKKVKKEIKSPKNSISEIKSPKKGKVKKEEETVWEWWKEEKRAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEIATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQNYFKKLSEDKKALPKEEKEKNKLKNEEIINQYGYCMMDSYKQKIGNFKIEPPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRHDPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDKIRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSLRVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEGKQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKIVADNVSNKVLAYNSANRQVAILCNHQRAVPKTFEKSMENLSEKIDKKKKDYSEVKDSAKKLKAEYSKSKDSGIKEKYEKLKKKLEKTKEMLNKMINQQTDREKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVDMTTENYKFQDLNSDKSTNVLK 671
The following BLAST results are available for this feature:
BLAST of DNA topoisomerase I vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TOP10.000e+061.96DNA topoisomerase I [Source:HGNC Symbol;Acc:HGNC:1... [more]
TOP1MT0.000e+057.43DNA topoisomerase I mitochondrial [Source:HGNC Sym... [more]
TOP1MT0.000e+058.05DNA topoisomerase I mitochondrial [Source:HGNC Sym... [more]
TOP1MT0.000e+058.05DNA topoisomerase I mitochondrial [Source:HGNC Sym... [more]
TOP1MT0.000e+058.05DNA topoisomerase I mitochondrial [Source:HGNC Sym... [more]
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BLAST of DNA topoisomerase I vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
top-10.000e+054.34DNA topoisomerase 1 [Source:UniProtKB/Swiss-Prot;... [more]
top-10.000e+054.34DNA topoisomerase 1 [Source:UniProtKB/Swiss-Prot;... [more]
top-10.000e+054.34DNA topoisomerase 1 [Source:UniProtKB/Swiss-Prot;... [more]
top-10.000e+054.34DNA topoisomerase 1 [Source:UniProtKB/Swiss-Prot;... [more]
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BLAST of DNA topoisomerase I vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Top10.000e+059.64gene:FBgn0004924 transcript:FBtr0100294[more]
Top10.000e+059.64gene:FBgn0004924 transcript:FBtr0074005[more]
Top10.000e+059.64gene:FBgn0004924 transcript:FBtr0308699[more]
Top10.000e+059.64gene:FBgn0004924 transcript:FBtr0074006[more]
Top10.000e+059.64gene:FBgn0004924 transcript:FBtr0340434[more]
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BLAST of DNA topoisomerase I vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
top1l0.000e+063.59DNA topoisomerase I, like [Source:ZFIN;Acc:ZDB-GEN... [more]
top10.000e+062.14DNA topoisomerase I [Source:ZFIN;Acc:ZDB-GENE-0301... [more]
zgc:1737420.000e+060.79zgc:173742 [Source:ZFIN;Acc:ZDB-GENE-030131-6489][more]
top15.820e-17865.55DNA topoisomerase I [Source:ZFIN;Acc:ZDB-GENE-0301... [more]
top1mt5.837e-16949.55DNA topoisomerase I mitochondrial [Source:ZFIN;Acc... [more]
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BLAST of DNA topoisomerase I vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
TOP10.000e+060.83DNA topoisomerase I, gene 1 [Source:NCBI gene;Acc:... [more]
TOP10.000e+060.83DNA topoisomerase I, gene 1 [Source:NCBI gene;Acc:... [more]
TOP10.000e+060.83DNA topoisomerase I, gene 1 [Source:NCBI gene;Acc:... [more]
TOP10.000e+060.83DNA topoisomerase I, gene 1 [Source:NCBI gene;Acc:... [more]
TOP10.000e+060.83DNA topoisomerase I, gene 1 [Source:NCBI gene;Acc:... [more]
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BLAST of DNA topoisomerase I vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Top10.000e+062.32topoisomerase (DNA) I [Source:MGI Symbol;Acc:MGI:9... [more]
Top1mt0.000e+055.64DNA topoisomerase 1, mitochondrial [Source:MGI Sym... [more]
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BLAST of DNA topoisomerase I vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P11387|TOP1_HUMAN0.000e+061.96DNA topoisomerase 1 OS=Homo sapiens OX=9606 GN=TOP... [more]
sp|Q7YR26|TOP1_CHLAE0.000e+061.96DNA topoisomerase 1 OS=Chlorocebus aethiops OX=953... [more]
sp|Q04750|TOP1_MOUSE0.000e+062.32DNA topoisomerase 1 OS=Mus musculus OX=10090 GN=To... [more]
sp|Q9WUL0|TOP1_RAT0.000e+062.32DNA topoisomerase 1 OS=Rattus norvegicus OX=10116 ... [more]
sp|Q07050|TOP1_CRIGR0.000e+062.32DNA topoisomerase 1 OS=Cricetulus griseus OX=10029... [more]
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BLAST of DNA topoisomerase I vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0X3NSW10.000e+067.15DNA topoisomerase I OS=Schistocephalus solidus OX=... [more]
A0A074Z8H70.000e+065.43DNA topoisomerase I OS=Opisthorchis viverrini OX=6... [more]
A0A430QRQ70.000e+066.49DNA topoisomerase I OS=Schistosoma bovis OX=6184 G... [more]
A0A4S2LVU20.000e+065.78DNA topoisomerase I OS=Opisthorchis felineus OX=14... [more]
H2KNI90.000e+065.43DNA topoisomerase I OS=Clonorchis sinensis OX=7992... [more]
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BLAST of DNA topoisomerase I vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
zgc:1737420.000e+059.96DNA topoisomerase I, mitochondrial-like [Source:NC... [more]
top10.000e+058.70DNA topoisomerase I [Source:ZFIN;Acc:ZDB-GENE-0301... [more]
ENSAMXT00000022486.20.000e+060.00DNA topoisomerase 1-like [Source:NCBI gene;Acc:103... [more]
zgc:1737420.000e+060.50DNA topoisomerase I, mitochondrial-like [Source:NC... [more]
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BLAST of DNA topoisomerase I vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
top10.000e+061.96DNA topoisomerase I [Source:ZFIN;Acc:ZDB-GENE-0301... [more]
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BLAST of DNA topoisomerase I vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
TOP19.686e-14752.14Topoisomerase I; nuclear enzyme that relieves tors... [more]
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BLAST of DNA topoisomerase I vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO354330.000e+059.96Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DNA topoisomerase I vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
top1l0.000e+062.86DNA topoisomerase I [Source:NCBI gene;Acc:10116405... [more]
top1l0.000e+062.86DNA topoisomerase I [Source:NCBI gene;Acc:10116405... [more]
top10.000e+062.86DNA topoisomerase I [Source:ZFIN;Acc:ZDB-GENE-0301... [more]
zgc:1737420.000e+059.75DNA topoisomerase I, mitochondrial [Source:NCBI ge... [more]
zgc:1737420.000e+059.75DNA topoisomerase I, mitochondrial [Source:NCBI ge... [more]
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BLAST of DNA topoisomerase I vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000055403.10.000e+099.70SMESG000055403.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002736 ID=SMED30002736|Name=DNA topoisomerase I|organism=Schmidtea mediterranea sexual|type=transcript|length=2519bp
TCTATATAAATCTTATATGAATTAAATAATGTCAAAAATTGCAATTAATG
GTAATGGTGCAACAATAAAATTAAAGAAAGAAATCGTTTCTGACGATGAC
GATGTTCCTCTTTCAAAACGAGTTACAAATGAGGCAAATGTTAGTGAGGA
TGATATGCCATTATTCAATAATCGTAAAAGAATATCTAAAGCATCTGATA
GATCATCACAACCAGAAAAAAAGAAAGTTAAGAAAGAAATAAAATCTCCT
AAAAACAGTATATCAGAAATAAAATCCCCTAAAAAAGGAAAAGTAAAGAA
AGAAGAGGAAACTGTTTGGGAATGGTGGAAAGAAGAAAAAAGAGCTGGTG
GAGATAAGTGGCAATTCCTTGAACATGCTGGACCAATGTTTGAGCTAGCA
TACGAACCGCTACCTAGCCATGTGAAGTTTTACTATGACGGAAAACATAT
GAAATTAAAACCAGAAACTGAAGAAATTGCTACATTTTATGCTAAAATGT
TTGACCACGATTACACCAAAAACGACGTTTTCAACAAAAATTTTATGCGA
GATTGGATAGCTGTAATGTCAAAAGACGAAAAATCTGTGATAAAAGATCT
AAGCAAATGCGATTTTAAACCAATGCAAAATTATTTCAAGAAACTAAGTG
AAGATAAAAAAGCATTACCAAAAGAAGAAAAAGAAAAAAATAAATTGAAA
AATGAAGAAATTATAAATCAATACGGATACTGTATGATGGACTCTTACAA
ACAGAAAATAGGCAACTTCAAAATTGAGCCACCTGGATTATTCCGCGGTC
GAGGAAATCATCCGAAAATGGGTTCAGTTAAGCGTCGAATTGTACCAGAA
GATGTGATAATTAATTGTTCTAAAAACTCAAAAATACCAAAACCACCGGC
TGGTCGCAAATGGAAAGAAGTTCGTCACGATCCCACTGTTACCTGGCTGG
CTTGTTGGCAGGAAAATGTGCAAAACAATTTCAAATATGTCATGTTAAAT
GCTTCATCAAGATTAAAAGGAGAAAAGGATTGGAAAAAATATGAAACTGC
CAGAAAATTGCATGAAAAAATCGATAAAATACGTCGAGATTACACAAACG
ATTTTAGTCATCGAGAAATGCGCATTCGACAGCGAGCTGTGGCCTTGTAT
TTTATTGACAAACTGGCATTGAGAGCTGGTAATGAGAAAGGTGAAGATGA
AGCTGATACTGTTGGCTGTTGTTCTTTACGGGTCGAGCATATCACTTTGG
AGGTCGATTCAAGCACAGACGAATACATCGTAAAGTTCGATTTCCTCGGT
AAAGATTCGATTCGGTACCAAAATGAAGTCTCGGTTCCGAAAAAAGTCTA
CAAGAATCTGTTGCGATTTAAAGAAGGAAAACAAGAGGGAGATGATTTGT
TTGACAGATTAAACACTAGCACATTAAATCAATATCTCCGGGAACTGATG
CCCGGACTAACAGCAAAAGTCTTCCGTACTTATAACGCTAGTATCACTCT
GGAAAGGGAGCTTGTAAATAAAAAGATTGTCGCCGATAATGTCTCTAACA
AAGTTCTAGCATACAATTCAGCCAATCGGCAAGTCGCTATACTCTGTAAC
CATCAACGGGCTATTCCGAAAACCTTTGAAAAATCGATGGAAAACTTATC
AGAAAAGATTGATAAGAAAAAGAAAGACTATTCCGAAGTGAAAGATTCAG
CCAAAAAATTAAAAGCAGAATATTCTAAATCAAAAGATTCTGGAATCAAA
GAGAAATATGAGAAATTGAAGAAGAAACTGGAAAAAACAAAGGAAATGTT
AAATAAAATGATAAATCAACAAACTGATCGAGAAAAGAATAAAGATGTTG
CACTTGGAACTAGTAAATTGAACTATCTTGATCCTCGAATTTCGATTGCC
TGGTGTCTCAAGAATAATGTTCCATTGAACAAAATCTACAACAAAACTCA
GCTTCAGAAATTCCAATGGGCAGTCGACATGACTACCGAAAATTATAAAT
TCCAGGATTTAAATTCGGACAAATCGACTAATGTTCTGAAGGAATGTGAT
ATTAATGATAATGATGAGGATGATGATGAAGATGAGTATGAATGATAGCG
TTGTTTTGAATGTAATTGTTTTGTTTTTTCTTTTCTGTGAAACTCTTTTC
AATCTGTACTCTAAAATTCGATAAATTGCGACCGAGTTCTACTGGTTTGT
ATGAGCGCATGTGTGATCGCGAATGCGTGTGTGCGATCACCCGCGTGACG
GTGTGTGATTGAATTAGTAATTGTATTTGATATCATTTCTTTTCTTATCC
ATTTCTGTGTGTTGATTTCATTGATAACTGACTGACCACGTCGACGACAA
AAGGAAGAGCTGAGGATGCTGATTGGATAATTGTAGTAATATGTTACTGA
AAACAAATATAATTTTGCATGTTAATGTAGTTCTAATTAATGTTATTTAT
ATATGCACTTCATTTCGTGTTGATTTGCATATAACAATTATTTTAATAAA
TTGATGATTTTCAATAAAA
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protein sequence of SMED30002736-orf-1

>SMED30002736-orf-1 ID=SMED30002736-orf-1|Name=SMED30002736-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=689bp
MSKIAINGNGATIKLKKEIVSDDDDVPLSKRVTNEANVSEDDMPLFNNRK
RISKASDRSSQPEKKKVKKEIKSPKNSISEIKSPKKGKVKKEEETVWEWW
KEEKRAGGDKWQFLEHAGPMFELAYEPLPSHVKFYYDGKHMKLKPETEEI
ATFYAKMFDHDYTKNDVFNKNFMRDWIAVMSKDEKSVIKDLSKCDFKPMQ
NYFKKLSEDKKALPKEEKEKNKLKNEEIINQYGYCMMDSYKQKIGNFKIE
PPGLFRGRGNHPKMGSVKRRIVPEDVIINCSKNSKIPKPPAGRKWKEVRH
DPTVTWLACWQENVQNNFKYVMLNASSRLKGEKDWKKYETARKLHEKIDK
IRRDYTNDFSHREMRIRQRAVALYFIDKLALRAGNEKGEDEADTVGCCSL
RVEHITLEVDSSTDEYIVKFDFLGKDSIRYQNEVSVPKKVYKNLLRFKEG
KQEGDDLFDRLNTSTLNQYLRELMPGLTAKVFRTYNASITLERELVNKKI
VADNVSNKVLAYNSANRQVAILCNHQRAIPKTFEKSMENLSEKIDKKKKD
YSEVKDSAKKLKAEYSKSKDSGIKEKYEKLKKKLEKTKEMLNKMINQQTD
REKNKDVALGTSKLNYLDPRISIAWCLKNNVPLNKIYNKTQLQKFQWAVD
MTTENYKFQDLNSDKSTNVLKECDINDNDEDDDEDEYE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003916DNA topoisomerase activity
GO:0003917DNA topoisomerase type I activity
GO:0003677DNA binding
GO:0003918DNA topoisomerase type II (ATP-hydrolyzing) activity
GO:0016853isomerase activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0002111X2 cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036202TopoI_DNA-bd_euk_N_sf
IPR001631TopoI
IPR013499TopoI_euk
IPR025834TopoI_C_dom
IPR013030DNA_topo_DNA_db_N_dom2
IPR014727TopoI_cat_a/b-sub_euk
IPR014711TopoI_cat_a-hlx-sub_euk
IPR013500TopoI_cat_euk
IPR008336TopoI_DNA-bd_euk
IPR013034DNA_topo_DNA_db_N_dom1
IPR011010DNA_brk_join_enz
Vocabulary: biological process
TermDefinition
GO:0006265DNA topological change
Vocabulary: cellular component
TermDefinition
GO:0005694chromosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 206..226
NoneNo IPR availableCOILSCoilCoilcoord: 537..564
NoneNo IPR availableCOILSCoilCoilcoord: 574..601
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 24..86
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 44..86
NoneNo IPR availablePANTHERPTHR10290DNA TOPOISOMERASE Icoord: 18..656
NoneNo IPR availableCDDcd03488Topoisomer_IB_N_htopoI_likecoord: 110..324
e-value: 2.65168E-119
score: 353.183
NoneNo IPR availableSUPERFAMILYSSF46596Eukaryotic DNA topoisomerase I, dispensable insert domaincoord: 534..605
IPR001631DNA topoisomerase IPRINTSPR00416EUTPISMRASEIcoord: 368..387
score: 70.18
coord: 418..434
score: 64.92
coord: 391..405
score: 75.48
coord: 254..263
score: 70.36
coord: 476..490
score: 80.0
coord: 610..621
score: 72.92
IPR013499DNA topoisomerase I, eukaryotic-typeSMARTSM00435topeucoord: 254..630
e-value: 1.6E-234
score: 795.1
IPR014727DNA topoisomerase I, catalytic core, alpha/beta subdomainGENE3DG3DSA:1.10.132.10coord: 480..658
e-value: 2.9E-64
score: 218.1
IPR025834Topoisomerase I C-terminal domainPFAMPF14370Topo_C_assoccoord: 589..658
e-value: 3.6E-29
score: 100.5
IPR013500DNA topoisomerase I, catalytic core, eukaryotic-typePFAMPF01028Topoisom_Icoord: 326..556
e-value: 5.1E-98
score: 327.0
IPR013500DNA topoisomerase I, catalytic core, eukaryotic-typeCDDcd00659Topo_IB_Ccoord: 332..531
e-value: 4.13974E-82
score: 256.82
IPR008336DNA topoisomerase I, DNA binding, eukaryotic-typePFAMPF02919Topoisom_I_Ncoord: 110..323
e-value: 4.5E-94
score: 313.9
IPR013030DNA topoisomerase I, DNA binding, N-terminal domain 2GENE3DG3DSA:2.170.11.10coord: 98..323
e-value: 1.7E-97
score: 327.3
IPR014711DNA topoisomerase I, catalytic core, alpha-helical subdomain, eukaryotic-typeGENE3DG3DSA:3.90.15.10coord: 326..479
e-value: 3.6E-64
score: 217.5
IPR013034DNA topoisomerase I, DNA binding, N-terminal domain 1GENE3DG3DSA:1.10.10.41coord: 126..213
e-value: 1.7E-97
score: 327.3
IPR036202DNA topoisomerase I, DNA binding, eukaryotic-type, N-terminal domain superfamilySUPERFAMILYSSF56741Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragmentcoord: 96..324
IPR011010DNA breaking-rejoining enzyme, catalytic coreSUPERFAMILYSSF56349DNA breaking-rejoining enzymescoord: 326..656