SMED30002685
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppSub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30002685 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 3
Alignments
SMED30002685 aligns in the following genomic locations:
Homology
BLAST of SMED30002685 vs. TrEMBL
Match: A0A0L8FYC5 (Uncharacterized protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22004138mg PE=4 SV=1) HSP 1 Score: 60.077 bits (144), Expect = 1.203e-6 Identity = 45/126 (35.71%), Postives = 69/126 (54.76%), Query Frame = 2 Query: 371 FRSILLDEWVTFHPIRMNVTVKDYNDATAV-ELVFTVRSTDDVQSWFTANNLISSMPWDISFMKASNYTYFSVSGSPYGMRFYISTYNAICSIDKGFFGVSVQPMLCNYGTKWSNGTDFNLPQILF 745 FR LLD W PI V + Y TAV +VF R+T +++WF+ NNL SS PW ++ YFS++G RFY+S Y+ +CS D+G+ ++ P+ C++ +D+N P I + Sbjct: 111 FRHPLLDFWNRL-PIEQ-VKLVIYKKQTAVVTMVFDGRNTT-IENWFSPNNLKSS-PWKD--LEPGRTNYFSLAGFRSQRRFYVSNYHDVCSKDRGWLFITEPPVPCSWAK-----SDYN-PTIFY 224
BLAST of SMED30002685 vs. TrEMBL
Match: A0A1I8GLC5 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 3.728e-6 Identity = 51/158 (32.28%), Postives = 76/158 (48.10%), Query Frame = 2 Query: 263 VLFRLSSGIDEDPYLYLTTKNPSNNDVIPDLSVDGNFRSILLDE--WVTFHPIRMNVTVKDYNDATAV-ELVFTV-RSTDDV--QSWFTANNLISSMPWDISFMKASNYTYFSVSGSPYGMR-FYISTYNAICSIDKGFFGVSVQPMLCNYGTK-WSN 712 +L+ + SG + + TK PS D+ D S+ +RS LD W H + + +D V ++VF V R T V +WFT L+SS P+ + + Y YFSV+G R F+I+ A C+ D GF+ VS + C + K WS Sbjct: 1015 LLYTVKSGAGHEVFKTFRTKGPS--DIPADRSLPDLYRSDQLDALAWTLLHTGYKRIVAEMRSDGRRVAQVVFDVSRHTKSVGMTNWFTPTRLVSSYPYHLG---KTGYNYFSVAGHRAHKRTFFINKVYAGCAGDLGFWMVSDRRDPCKWANKGWSG 1167
BLAST of SMED30002685 vs. TrEMBL
Match: A0A1I8GLP2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1) HSP 1 Score: 58.9214 bits (141), Expect = 8.045e-6 Identity = 51/157 (32.48%), Postives = 76/157 (48.41%), Query Frame = 2 Query: 263 VLFRLSSGIDEDPYLYLTTKNPSNNDVIPDLSVDGNFRSILLDE--WVTFHPIRMNVTVKDYNDATAV-ELVFTV-RSTDDV--QSWFTANNLISSMPWDISFMKASNYTYFSVSGSPYGMR-FYISTYNAICSIDKGFFGVSVQPMLCNYGTK-WS 709 +L+ + SG + + TK PS D+ D S+ +RS LD W H + + +D V ++VF V R T V +WFT L+SS P+ + + Y YFSV+G R F+I+ A C+ D GF+ VS + C + K WS Sbjct: 1461 LLYTVKSGAGHEVFKTFRTKGPS--DIPADRSLPDLYRSDQLDALAWTLLHTGYKRIVAEMRSDGRRVAQVVFDVSRHTKSVGMTNWFTPTRLVSSYPYHLG---KTGYNYFSVAGHRAHKRSFFINKVYAGCAGDLGFWMVSDRRDPCKWANKGWS 1612
BLAST of SMED30002685 vs. TrEMBL
Match: C3XXV4 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_97527 PE=4 SV=1) HSP 1 Score: 58.151 bits (139), Expect = 8.781e-6 Identity = 35/134 (26.12%), Postives = 71/134 (52.99%), Query Frame = 2 Query: 263 VLFRLSSGIDEDPYLYLTTKNPSNNDVIPDLSVDGNFRSILLDEWVTFHPIRMNVTVKDYNDATAVELVFTVRSTDDVQSWFTANNLISSMPWDISFMKASNYTYFSVSG-SPYGMRFYISTYNAICSIDKGFF 661 ++F++ +G+ Y ++ N+ V P L+ ++RS LD W + H ++ V++ + T +++F ++ +WF+ + LISS PW + +K + +FS+ G +G RF+I+ C+ D G+ Sbjct: 147 MVFKIVAGVPGSSYALWRSRGGYNDQVHPSLTGHSHYRSPDLDRWSSLHVTKVKVSLVTQHQHTR-DIIFNAHGSNR-DNWFSKDRLISS-PW--TDLKTAPANFFSIQGDDRHGRRFFINRSYGGCAHDWGWL 275
BLAST of SMED30002685 vs. Planmine SMEST
Match: SMESG000065828.1 (SMESG000065828.1) HSP 1 Score: 531.946 bits (1369), Expect = 0.000e+0 Identity = 261/265 (98.49%), Postives = 262/265 (98.87%), Query Frame = 2 Query: 56 MTIYFYLALSLLLFRPLDSKTKLNCALSASNIKQYFVRINDTCFLPQAKVYPVGGKLSSRIFKKIDSSIVLFRLSSGIDEDPYLYLTTKNPSNNDVIPDLSVDGNFRSILLDEWVTFHPIRMNVTVKDYNDATAVELVFTVRSTDDVQSWFTANNLISSMPWDISFMKASNYTYFSVSGSPYGMRFYISTYNAICSIDKGFFGVSVQPMLCNYGTKWSNGTDFNLPQILFVLNNRGTDDLMQSFTRPLKYLEIAGTYDSDVSIFY 850 MTIYFYLALSLLL RPLDSKTKLNCALSASNIKQYFVRINDTCFLPQAKVYPVGGKL+SRIFKKIDSSIVLFRLSSGIDEDPYLYLTTKNPSNNDVIPDLSVDGNFRSILLDEWVTFHPIRMNVTVKDYN ATAVELVFTVRSTDDVQSWFTANNLISSMPWDISFMKASNYTYFSVSGSPYGMRFYISTYNAICSIDKGFFGVSVQPMLCNYGTKWSNGTDFNLPQILFVLNNRGTDDLMQSFTRPLKYLEI GTYDSDVSIFY Sbjct: 1 MTIYFYLALSLLLSRPLDSKTKLNCALSASNIKQYFVRINDTCFLPQAKVYPVGGKLASRIFKKIDSSIVLFRLSSGIDEDPYLYLTTKNPSNNDVIPDLSVDGNFRSILLDEWVTFHPIRMNVTVKDYNYATAVELVFTVRSTDDVQSWFTANNLISSMPWDISFMKASNYTYFSVSGSPYGMRFYISTYNAICSIDKGFFGVSVQPMLCNYGTKWSNGTDFNLPQILFVLNNRGTDDLMQSFTRPLKYLEITGTYDSDVSIFY 265
BLAST of SMED30002685 vs. Planmine SMEST
Match: SMESG000065749.1 (SMESG000065749.1) HSP 1 Score: 212.231 bits (539), Expect = 7.787e-68 Identity = 110/240 (45.83%), Postives = 145/240 (60.42%), Query Frame = 2 Query: 107 DSKTKLNCALSASNIKQYFVRINDTCFLPQAKVYPVGGKLSSRIFKKIDSSIVLFRLSSGIDEDPYLYLTTKNPSNNDVIPDLSVDGNFRSILLDEWVTFHPIRMNVTVKDYNDATAVELVFTVRSTDDVQSWFTANNLISSMPWDISFMKASNYTYFSVSGSPYGMRFYISTYNAICSIDKGFFGVSVQPMLCNYGTKWSNGTDFNLPQILFVLNNRGTDDLMQSFTRPLKYLEIAGTY 826 D + C L A N + YF IN+ C+ PQ + KL R + K+++S V+FRL SG DPY Y +T N S + V D + NFRSI+LDEW P R+ + DYND AV L F V TD + SWFT +N S PWDI+F++ NYTYFS+ G+ +GM FYI+ N +C +D G FG++ M C + W NGT F PQI++VLNN T DL ++F R LKYLEI+G Y Sbjct: 18 DRNIAIECGLIALNGQMYFAVINNVCYFPQTPSSIINSKLLQRSYIKLNNSFVIFRLVSGSGVDPYGYWSTTNVSIHTVADDQNKLMNFRSIILDEWNNIKPSRLTLVAFDYNDQVAVILGFNVFQTDSITSWFTKSNFNYSFPWDINFLQTINYTYFSLIGASFGMCFYINQINDVCIVDSGIFGIAKPNMPC---SMWPNGTSFTYPQIMYVLNNERTPDLWKNFERKLKYLEISGEY 254
BLAST of SMED30002685 vs. Planmine SMEST
Match: SMESG000065776.1 (SMESG000065776.1) HSP 1 Score: 196.823 bits (499), Expect = 1.244e-61 Identity = 105/247 (42.51%), Postives = 147/247 (59.51%), Query Frame = 2 Query: 113 KTKLNCALSASNIKQYFVRINDTCFLPQAKVYPVGGKLSSRIFKKIDSSIVLFRLSSGIDEDPYLYLTTKNPSNNDVIPDLSVDGNFRSILLDEWVTFHPIRMNVTVKDYNDATAVELVFTVRSTDDVQSWFTANNLISSMPWDISFMKASNYTYFSVSGSPYGMRFYISTYNAICSIDKGFFGVSVQPMLCN--YGTKWSNGTDFNLPQILFVLNNRGTDDLMQSFTRPLKYLEIAGTYDSDVSIF 847 K C L N + +F IN+ C++ QA V L R + + + +VLFRL SG DPYLY +T N S + + + NFRSI+LDEW P+ M +T DYND +V L F V ++D + SWFT +N+ SS PW+ ++++ +Y YFS+SG+ +GMRFYI+ N C D GFFG+ C+ YG W NGT F LPQI+++LN T DL +++ PLKYLEI+G YD + F Sbjct: 21 KNVYKCGLETMNKRVFFAIINNICYISQAPSSIVTSTLQWRSYSNLFNKMVLFRLVSGSGVDPYLYWSTTNRSIHTAADNSNKLQNFRSIILDEWDYVKPLIMTLTAFDYNDQISVILEFNVLASDSITSWFTKSNIKSSYPWNSTYLQTLSYFYFSMSGTSWGMRFYINIVNINCYRDAGFFGILSAVTGCSNQYGGFWPNGTAFILPQIMYILNVEKTYDLWYNYSYPLKYLEISGQYDKNTIFF 267
BLAST of SMED30002685 vs. Planmine SMEST
Match: SMESG000065824.1 (SMESG000065824.1) HSP 1 Score: 191.815 bits (486), Expect = 1.188e-59 Identity = 103/248 (41.53%), Postives = 144/248 (58.06%), Query Frame = 2 Query: 113 KTKLNCALSASNIKQYFVRINDTCFLPQAKVYPVGGKLSSRIFKKIDSSIVLFRLSSGIDEDPYLYLTTKNPSNNDVIPDLSVDGNFRSILLDEWVTFHPIRMNVTVKDYNDATAVELVFTVRSTDDVQSWFTANNLISSMPWDISFMKASNYTYFSVSGSPYGMRFYISTYNAICSIDKGFFGVSVQPMLCN--YGTKWSNGTDFNLPQILFVLNNRGTDDLMQSFTRPLKYLEIAGTYDSDVSIFY 850 K C N + +F IN+ C++ QA V L R + + + +VLFRL S DPYLY +T N S + + NFRSI+LDEW P+ M +T DYND +V L F V ++D + SWF +N+ SS PW+ ++++ +Y YFS+SG+ +GMRFYI+ N C D GFFG+ C YG W NGT LPQI+++LN T DL +++ PLKYLEI+G YD + SIF+ Sbjct: 21 KNVYKCGFQTMNKRVFFAIINNICYISQAPSSIVTSTLQWRSYSNLFNKMVLFRLVSVSGVDPYLYWSTTNSSIQTAADNSNKLQNFRSIILDEWDYVKPLIMTLTAFDYNDQVSVILEFNVLASDSITSWFIKSNIKSSYPWNSTYLRTLSYYYFSMSGTSWGMRFYINIVNIYCYTDAGFFGILTTATGCPNLYGGFWPNGTALQLPQIMYILNVEKTYDLWYNYSYPLKYLEISGQYDKN-SIFF 267
BLAST of SMED30002685 vs. Planmine SMEST
Match: SMESG000065804.1 (SMESG000065804.1) HSP 1 Score: 169.088 bits (427), Expect = 1.360e-51 Identity = 90/199 (45.23%), Postives = 124/199 (62.31%), Query Frame = 2 Query: 260 IVLFRLSSGIDEDPYLYLTTKNPSNNDVIPDLSVDGNFRSILLDEWVTFHPIRMNVTVKDYNDATAVELVFTVRSTDDVQSWFTANNLISSMPWDISFMKASNYTYFSVSGSPYGMRFYISTYNAICSIDKGFFGVSVQPMLCN--YGTKWSNGTDFNLPQILFVLNNRGTDDLMQSFTRPLKYLEIAGTYDSDVSIFY 850 + + RL SG DPYLY +T N S + + NFRSI+LDEW P+ M +T DYND +V L F V ++D + SWFT +N+ SS PW+ ++++ +Y YFS+SG+ +GMRFYI+ N C D GFFG+ C YG W NGT LPQI+++LN T DL +++ PLKYLEI+G YD + SIF+ Sbjct: 15 VEIHRLVSGSGVDPYLYWSTTNSSIQTAADNSNKLQNFRSIILDEWDYVKPLIMTLTAFDYNDQISVILEFNVLASDSITSWFTESNIKSSYPWNSTYLQTLSYYYFSMSGTSWGMRFYINIANINCYTDAGFFGILTTAHDCPNLYGGFWPNGTALQLPQIMYILNVEKTYDLWYNYSYPLKYLEISGQYDKN-SIFF 212 The following BLAST results are available for this feature:
BLAST of SMED30002685 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30002685 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30002685 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30002685 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30002685 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30002685 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30002685 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30002685 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 4
BLAST of SMED30002685 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30002685 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30002685 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30002685 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30002685 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30002685 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30002685 ID=SMED30002685|Name=SMED30002685|organism=Schmidtea mediterranea sexual|type=transcript|length=892bpback to top protein sequence of SMED30002685-orf-1 >SMED30002685-orf-1 ID=SMED30002685-orf-1|Name=SMED30002685-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=266bp MTIYFYLALSLLLFRPLDSKTKLNCALSASNIKQYFVRINDTCFLPQAKVback to top InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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