1.G9.2

Overview
Name1.G9.2 (AHD24735.1)
Smed IDSMED30002682
Length (bp)684
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 1.G9.2 (SMED30002682) t-SNE clustered cells

Violin plots show distribution of expression levels for 1.G9.2 (SMED30002682) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002682

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 17

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
tail regionSMED30002682SMESG000033795.1 SmedASXL_005535SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30002682SMESG000033798.1 SMESG000033795.1 dd_Smed_v4_3199_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30002682SMESG000033798.1 SMESG000033795.1 dd_Smed_v4_3199_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
dorsal ventral margin of the whole animalSMED30002682SMESG000033798.1 SMESG000033795.1 dd_Smed_v4_3199_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
esophagusSMED30002682SMESG000033798.1 SMESG000033795.1 dd_Smed_v4_3199_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
pharynx tipSMED30002682SMESG000033798.1 SMESG000033795.1 dd_Smed_v4_3199_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult fluorescence in situ hybridization evidence
pharynxSMED30002682SMESG000033795.1 dd_Smed_v4_3234_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30002682SMESG000033795.1 dd_Smed_v4_3234_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002682SMESG000033795.1 Contig47962newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002682SMESG000033795.1 Contig47962uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002682SMESG000033798.1 SMESG000033795.1 Contig12940newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002682SMESG000033798.1 SMESG000033795.1 Contig12940uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30002682SMESG000033798.1 SMESG000033795.1 dd_Smed_v6_3199_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30002682SMESG000033795.1 dd_Smed_v6_3234_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
reproductive organSMED30002682SMESG000033795.1 Contig47962newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30002682SMESG000033795.1 Contig47962uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
dorsal ventral margin of the whole animalSMED30002682SMESG000033795.1 dd_Smed_v4_3234_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
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BLAST of 1.G9.2 vs. TrEMBL
Match: V9XMR4 (1.G9.2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 388.267 bits (996), Expect = 3.221e-135
Identity = 214/225 (95.11%), Postives = 214/225 (95.11%), Query Frame = 3 
Query:   12 MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEATEVDIDKKLKHHKRFLAXXXXXXXXXXXXXXXX----------GEKKINTNSLNHPKDSFEHLNKIRILNCTIESLANKIQLIKTNKEVIKRYNEELKKMETLCGKKRNDYMKKCFKELKSVIFDLDKCEDYLEVADQFCKRQRSNLIYNRQNETLSNISDYYSKKNKLIEKCQNHLQ 656
            MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEATEVDIDKKLKHHKRFLATNNKSKEKTNTNDKSK          GEKKINTNSLNHPKDSFEHLNKIRILNCTIE LANKIQLIKTNKEVIKRYNEELKKMETLCGKKRNDYMKKCFKELKSVIFDLDKCEDYLEVADQFCKRQRSNLIYNRQNETLSNISDYYSKKNKLIEKCQNHLQ
Sbjct:    1 MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEATEVDIDKKLKHHKRFLATNNKSKEKTNTNDKSKEKTNTNDKSKGEKKINTNSLNHPKDSFEHLNKIRILNCTIEGLANKIQLIKTNKEVIKRYNEELKKMETLCGKKRNDYMKKCFKELKSVIFDLDKCEDYLEVADQFCKRQRSNLIYNRQNETLSNISDYYSKKNKLIEKCQNHLQ 225
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BLAST of 1.G9.2 vs. Planmine SMEST
Match: SMESG000033795.1 (SMESG000033795.1)

HSP 1 Score: 389.423 bits (999), Expect = 2.623e-139
Identity = 215/225 (95.56%), Postives = 215/225 (95.56%), Query Frame = 3 
Query:   12 MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEATEVDIDKKLKHHKRFLAXXXXXXXXXXXXXXXX----------GEKKINTNSLNHPKDSFEHLNKIRILNCTIESLANKIQLIKTNKEVIKRYNEELKKMETLCGKKRNDYMKKCFKELKSVIFDLDKCEDYLEVADQFCKRQRSNLIYNRQNETLSNISDYYSKKNKLIEKCQNHLQ 656
            MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEATEVDIDKKLKHHKRFLATNNKSKEKTNTNDKSK          GEKKINTNSLNHPKDSFEHLNKIRILNCTIESLANKIQLIKTNKEVIKRYNEELKKMETLCGKKRNDYMKKCFKELKSVIFDLDKCEDYLEVADQFCKRQRSNLIYNRQNETLSNISDYYSKKNKLIEKCQNHLQ
Sbjct:    1 MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEATEVDIDKKLKHHKRFLATNNKSKEKTNTNDKSKEKTNTNDKSKGEKKINTNSLNHPKDSFEHLNKIRILNCTIESLANKIQLIKTNKEVIKRYNEELKKMETLCGKKRNDYMKKCFKELKSVIFDLDKCEDYLEVADQFCKRQRSNLIYNRQNETLSNISDYYSKKNKLIEKCQNHLQ 225
[Close]
BLAST of 1.G9.2 vs. Planmine SMEST
Match: SMESG000033795.1 (SMESG000033795.1)

HSP 1 Score: 302.368 bits (773), Expect = 5.090e-105
Identity = 171/182 (93.96%), Postives = 171/182 (93.96%), Query Frame = 3 
Query:   12 MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEATEVDIDKKLKHHKRFLAXXXXXXXXXXXXXXXX----------GEKKINTNSLNHPKDSFEHLNKIRILNCTIESLANKIQLIKTNKEVIKRYNEELKKMETLCGKKRNDYMKKCFKELKSVIFDLDKCEDYLEV 527
            MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEATEVDIDKKLKHHKRFLATNNKSKEKTNTNDKSK          GEKKINTNSLNHPKDSFEHLNKIRILNCTIESLANKIQLIKTNKEVIKRYNEELKKMETLCGKKRNDYMKKCFKELKSVIFDLDKCEDYLE 
Sbjct:    1 MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEATEVDIDKKLKHHKRFLATNNKSKEKTNTNDKSKEKTNTNDKSKGEKKINTNSLNHPKDSFEHLNKIRILNCTIESLANKIQLIKTNKEVIKRYNEELKKMETLCGKKRNDYMKKCFKELKSVIFDLDKCEDYLET 182

HSP 2 Score: 72.0182 bits (175), Expect = 3.151e-15
Identity = 34/34 (100.00%), Postives = 34/34 (100.00%), Query Frame = 2 
Query:  575 TKRDVIKYFRLLLKEKQTNRKMPKSSPMTYLFEN 676
            TKRDVIKYFRLLLKEKQTNRKMPKSSPMTYLFEN
Sbjct:  182 TKRDVIKYFRLLLKEKQTNRKMPKSSPMTYLFEN 215
[Close]
BLAST of 1.G9.2 vs. Planmine SMEST
Match: SMESG000033798.1 (SMESG000033798.1)

HSP 1 Score: 111.694 bits (278), Expect = 2.191e-30
Identity = 52/53 (98.11%), Postives = 53/53 (100.00%), Query Frame = 3 
Query:   12 MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEATEVDID 170
            MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLP+ATEVDID
Sbjct:    1 MRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPKATEVDID 53
The following BLAST results are available for this feature:
BLAST of 1.G9.2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
V9XMR43.221e-13595.111.G9.2 OS=Schmidtea mediterranea OX=79327 PE=2 SV=... [more]
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BLAST of 1.G9.2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 1.G9.2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000033795.12.623e-13995.56SMESG000033795.1[more]
SMESG000033795.15.090e-10593.96SMESG000033795.1[more]
SMESG000033798.12.191e-3098.11SMESG000033798.1[more]
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Cross References
External references for this transcript
DatabaseAccession
SmedGD GBrowseSMED30002682
GenBankAHD24735.1
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002682 ID=SMED30002682|Name=1.G9.2|organism=Schmidtea mediterranea sexual|type=transcript|length=684bp
AAACATTTGCAATGAGAATCATTTTTGCAATTTTCGTTTTTATAGTATCG
TATCAAGTAGTATTCGGTGAATATGACTTAGATCATTTGAAACAGGAGCT
CGAAGAAAAGGTCAATCAATTAAAACAAGAATCTCAACAATTACCGGAGG
CAACAGAGGTAGATATAGACAAGAAATTAAAACATCACAAAAGATTTTTA
GCAACAAATAATAAATCCAAAGAGAAAACAAACACAAATGATAAATCCAA
AGGGGAAAAAAAGATAAATACAAATTCCTTGAACCACCCAAAGGACAGTT
TCGAACATTTAAACAAAATTAGAATTTTGAATTGTACAATCGAAAGTTTG
GCAAATAAAATACAATTAATTAAAACAAACAAAGAAGTTATTAAAAGATA
TAATGAGGAATTAAAAAAAATGGAGACATTGTGCGGAAAAAAAAGGAATG
ATTACATGAAAAAATGTTTCAAAGAACTAAAATCTGTTATATTCGATCTG
GATAAATGCGAGGATTATTTGGAGGTTGCAGACCAATTTTGTAAAAGACA
ACGTTCTAATTTAATTTATAATAGACAAAACGAGACGTTATCAAATATTT
CAGATTATTACTCAAAGAAAAACAAACTAATAGAAAAATGCCAAAATCAT
CTCCAATGACGTATTTATTTGAAAATTAGTGACC
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protein sequence of SMED30002682-orf-1

>SMED30002682-orf-1 ID=SMED30002682-orf-1|Name=SMED30002682-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=219bp
TFAMRIIFAIFVFIVSYQVVFGEYDLDHLKQELEEKVNQLKQESQQLPEA
TEVDIDKKLKHHKRFLATNNKSKEKTNTNDKSKGEKKINTNSLNHPKDSF
EHLNKIRILNCTIESLANKIQLIKTNKEVIKRYNEELKKMETLCGKKRND
YMKKCFKELKSVIFDLDKCEDYLEVADQFCKRQRSNLIYNRQNETLSNIS
DYYSKKNKLIEKCQNHLQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000062esophagus
PLANA:0000421tail
PLANA:0002032epidermal cell
PLANA:0002074pharynx tip
PLANA:0002089reproductive organ
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 133..160
NoneNo IPR availableCOILSCoilCoilcoord: 23..53
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 74..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 64..105
NoneNo IPR availableSIGNALP_EUKSignalP-TMSignalP-TMcoord: 1..22
score: 0.56
NoneNo IPR availableTMHMMTMhelixcoord: 2..21