Cilia-and flagella-associated protein 74
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30002606 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 20Note: Hover over icons to view figure legend
Homology
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Human
Match: CFAP74 (cilia and flagella associated protein 74 [Source:HGNC Symbol;Acc:HGNC:29368]) HSP 1 Score: 486.878 bits (1252), Expect = 2.487e-149 Identity = 294/916 (32.10%), Postives = 477/916 (52.07%), Query Frame = 2
Query: 2153 IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDS-YELVKPESIEI-------DSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDR--SSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIEL----SSHFYGQTNLT--------------TKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKT----DDNNEDEKPSLNTPIILKSAY---SDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVT 4795
I P+ ++ I ++P+ GD F++ F++ RV G AIDVPV++ +V + ICM +RLYQD+++ ++R+ AL+L+F V E++ +LE+LP TG+IQA ++ Q+K L R S+ + YFD ET +L+ + +A+Q K V F+ AI+TT+DL++ + +DFG CTI E I L N +LPQEFGF P+F+++QPN G+GTILP ETL+ V+F P KA+ + FQL C+S I+R F +SC +GVHP L+LS + F TA+ D S +++ N+HLS + TH P+IG P+GP +F+F++P +SPI I P G++ P ++ V V F P L I+ EA L+N+++E + +K N + K+ G S L A D +++ P +E+ S+++ + +LL F F+ V C +A+G I DR S + ++ +N+L+LEL CP++ P +++ S+ GK I +FG+ R K ISIQN+S E + ++LNPNG F + N L LV+SF + + Q + ++ L +T + A + ++ N G V+ E + NNS L + + + L S+ GQ L T+N N +F++ P +GV+ GK+Q V+FSPDH S F + ++ +F++ +++G M+++ P ++ P E++ D + +E L PI++ Y D A VGC+RT S KK E+SID++ L GF+++PS+G +E K+I+ISW+PP++++ L S L+ L+G+ T
Sbjct: 170 IGPFSSIKVPIVFTPVVPGDVQARFKVTFKNPQCPTLHFRVVGVAIDVPVWVPKPSVDLKICMYDRLYQDSVLVHTRSKAALRLKFEVCKELRAHLELLPKTGYIQAQSSYSVQLKFLPRHSLPEDAGRYFDKETRVLEAPMTIWVADQNKPVGFTVHAIVTTSDLEL-----SPSEVDFGYCTIYEAIRTEISLHNHSLLPQEFGFVRLPKFVDVQPNDGFGTILPLETLQFCVIFQPTKAEEHRFQLTCKSEINRCFKLSCRAVGVHPPLELSHYQIKFAATALYDTSVATVYVINSHLSMSSPTHSKPRIGSEDASPMGPTSFEFLLPPDSPITISPSVGTVWPGKRCLVQVAFRPVLPEKLIRQEALPLLNKEMETKSFRK---------------------------------NMAPQRKDLHGLSFSVLRAQNRDKLFKVSVPHVLEMRKRELRPSSDEYQAARATLLRAFQAKFDTFVVPCVVASGDIKDRKGSEPLSFSPHNTLYLELWCPTVAPSVVVTSHKGKTIFNFGDVAVGHRSIKKISIQNVSPEDLALDFSLLNPNGPFVLLNHSSLLRAGGTQVLVLSFSPHESILAQETLDII--TKRGTLTLTLMGTGV-ASMITCSIEGSVLNMGYVIAGESVSSGFKLQNNSLLPIKFSMHLDSLSSTRGRGQQQLPQFLSSPSQRTEVVGTQNLNGQSVFSVAPVKGVMDPGKTQDFTVTFSPDHESLYFSDKLQVVLFEKKISHQILLKGAACQHMMFVEGGDPLDV----------PVESLTAIPVFDPRHREEAEELR-PILVTLDYIQFDTDTPAPPATRELQVGCIRTTQPSPKKTVEFSIDSVASLQHKGFSIEPSRGSVERGQTKTISISWVPPADFDPDHPLMVSALLQLRGDVKET 1033 HSP 2 Score: 68.9366 bits (167), Expect = 8.168e-11 Identity = 30/75 (40.00%), Postives = 49/75 (65.33%), Query Frame = 2
Query: 1805 EPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTNCSIKNMSE 2029
+P +N+DL G I F +Q+ +F++P+KC TKKC +S+D+++I F + V+GE+ + + L N G GT SE
Sbjct: 2 KPMINKDLEGNISFLAQTGEFSVPLKCSTKKCSLSLDKELIDFGSYVVGETTSRTITLTNVGGLGTTFKFLPASE 76
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Human
Match: CFAP74 (cilia and flagella associated protein 74 [Source:HGNC Symbol;Acc:HGNC:29368]) HSP 1 Score: 442.965 bits (1138), Expect = 3.746e-129 Identity = 270/851 (31.73%), Postives = 435/851 (51.12%), Query Frame = 2
Query: 2153 IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDS-YELVKPESIEI-------DSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDR--SSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIEL----SSHFYGQTNL--------------TTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKT----DDNNEDEKPSLNTPIILKSAY---SDDGLIQEAKFCFTVGCVRTLAVSQKK 4600
I P+ ++ I ++P+ GD F++ F++ RV G AIDVPV++ +V + ICM +RLYQD+++ ++R+ AL+L+F V E++ +LE+LP TG+IQA ++ Q+K L R S+ + YFD ET +L+ + +A+Q K V F+ AI+TT+DL++ + +DFG CTI E I L N +LPQEFGF P+F+++QPN G+GTILP ETL+ V+F P KA+ + FQL C+S I+R F +SC +GVHP L+LS + F TA+ D S +++ N+HLS + TH P+IG P+GP +F+F++P +SPI I P G++ P ++ V V F P L I+ EA L+N+++E + +K N + K+ G S L A D +++ P +E+ S+++ + +LL F F+ V C +A+G I DR S + ++ +N+L+LEL CP++ P +++ S+ GK I +FG+ R K ISIQN+S E + ++LNPNG F + N L LV+SF + + Q + ++ L +T + A + ++ N G V+ E + NNS L + + + L S+ GQ L T+N N +F++ P +GV+ GK+Q V+FSPDH S F + ++ +F++ +++G M+++ P ++ P E++ D + +E L PI++ Y D A VGC+RT S KK
Sbjct: 753 IGPFSSIKVPIVFTPVVPGDVQARFKVTFKNPQCPTLHFRVVGVAIDVPVWVPKPSVDLKICMYDRLYQDSVLVHTRSKAALRLKFEVCKELRAHLELLPKTGYIQAQSSYSVQLKFLPRHSLPEDAGRYFDKETRVLEAPMTIWVADQNKPVGFTVHAIVTTSDLEL-----SPSEVDFGYCTIYEAIRTEISLHNHSLLPQEFGFVRLPKFVDVQPNDGFGTILPLETLQFCVIFQPTKAEEHRFQLTCKSEINRCFKLSCRAVGVHPPLELSHYQIKFAATALYDTSVATVYVINSHLSMSSPTHSKPRIGSEDASPMGPTSFEFLLPPDSPITISPSVGTVWPGKRCLVQVAFRPVLPEKLIRQEALPLLNKEMETKSFRK---------------------------------NMAPQRKDLHGLSFSVLRAQNRDKLFKVSVPHVLEMRKRELRPSSDEYQAARATLLRAFQAKFDTFVVPCVVASGDIKDRKGSEPLSFSPHNTLYLELWCPTVAPSVVVTSHKGKTIFNFGDVAVGHRSIKKISIQNVSPEDLALDFSLLNPNGPFVLLNHSSLLRAGGTQVLVLSFSPHESILAQETLDII--TKRGTLTLTLMGTGV-ASMITCSIEGSVLNMGYVIAGESVSSGFKLQNNSLLPIKFSMHLDSLSSTRGRGQQQLPQFLSSPSQRTEVVGTQNLNGQSVFSVAPVKGVMDPGKTQDFTVTFSPDHESLYFSDKLQVVLFEKKISHQILLKGAACQHMMFVEGGDPLDV----------PVESLTAIPVFDPRHREEAEELR-PILVTLDYIQFDTDTPAPPATRELQVGCIRTTQPSPKK 1551 HSP 2 Score: 206.453 bits (524), Expect = 9.973e-53 Identity = 155/592 (26.18%), Postives = 297/592 (50.17%), Query Frame = 2
Query: 269 QQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTN-RAVEKAHKKHS---EFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTAN-NLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTNCSIKNMSE 2029
++ + EL+ C ++K+ + EI T N+AA+ RLQ+ +R+ E+E E+DL+ + ++E H +++EG+L E +T ++ R + R ++ + AL +E ++ + L TQ+ L + + +E A K H FL+ ++ + R + + E E ++ +V L I ++ L + ++ L ++ +K+++ +G + + QR++ E+ QK K +K +I +I+ EE +K+ K P P+ L + + + + + D + T+ +E G S E + ++ I+ ++ +++T + PE GLW+ DYK + ++ K +KMD++I+ R + +L+ ++ Q+V + F+G F SKP ++ F++ ++ + Y++K+ L NT+Y+ N C+L V E+L DF+ + F PPGPLS GM+CE + F+P +N+DL G I F +Q+ +F++P+KC TKKC +S+D+++I F + V+GE+ + + L N G GT SE
Sbjct: 96 EKMRGELRACRQRRDLIDKQQEAVAAEIATEEEAGNMAAVGRLQAVSRRLFAELENERDLQSRTEAVLKESENTMWHIEIQEGRL----EAFRTADREEVEATGRRLQVRAAEQLCREQEALGKVERNRLLRIRKSLNTQKELGLRHQKLLEDARKNHKVAVRFLKASLGRIREQEKKEEMECHEYMRRRMDAVVALKGSISANRDTLRKFQAWDRAKAELAEQRVQAEKKAILAQGRDAFRHLVHQRRRQELEAQKRAFEEEQKLRKQEIISRILKEEAEEEKRKKQHP--------PTSARHRLTLRDKTWNYISDFCKKTTVPTNTYTLDYEAAAGPGPSRLLEVVSSEL-------------IQGDPGASSEEET-LAEPEISGLWNE--DYKPYQVPKEDVDRKPVGGTKMDKDILERTVERLRSRVVHKQVVWGREFQGRPFNSKPELLHFQDFDIGKVYKKKITLVNTTYTINYCKLVGVEEHLRDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGNISFLAQTGEFSVPLKCSTKKCSLSLDKELIDFGSYVVGETTSRTITLTNVGGLGTTFKFLPASE 659
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Zebrafish
Match: cacna1db (calcium channel, voltage-dependent, L type, alpha 1D subunit, b [Source:ZFIN;Acc:ZDB-GENE-040525-3]) HSP 1 Score: 563.533 bits (1451), Expect = 1.208e-172 Identity = 428/1578 (27.12%), Postives = 772/1578 (48.92%), Query Frame = 2
Query: 272 QFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKA---HKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKH-ISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLW-----------DTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTNCSIKNMSEID---------------NE-------------------TRKVVS--REPNSAVNIDVQTDLLDTKPITTSLDHLKLWIE--------PYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANK-------KWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGE-TVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNW-SLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSSHFYGQTNLTT------KNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGE 4783
Q K ELK H+SEL ++ E++E + N LFRL++QLKR+ E++ E+DL + + + E + ++E G+ + ++L+ +EK + ++++ Q+ R ++EK++ + D+ K++ Q+ +L + E+A H K + +L++T+++ + K+ A+E ++ +KK +V L + +E++ A ++K+ + ++ ++E ++++ + +G N + + Q +K+ E+ + ++++I K++ E + ++K K P + +P K+ + L E K ++ E+ S P+ SS + ++ F ++ D ++ PEF GLW D I+ + S T + SASK ++ + V + KG F SKP +I+F++ EV Y++KV LTN +Y TN C+L VS+NL D V++ F PPGP+S GM CE + F P ++ DL G I F S + F++P+KC KKC M +D+ + F T V+G+++ + + + N GA GT ++ +S + NE TR + S R P+ + D ++ ++TK + ++++ ++ I P+ + I ++P G+ +F+I F + + D+ G A VPV++ + + + IC +RLYQD I SRA+TAL+L F V E+++++ I P TG IQA +F AQ+K L R S+ + +F+ +TG+L+V + +A+QVK V F+ A++T++DL D E +DFG C+I E ++L N +LPQEFGF G P+FI++QPN G+GT+LP +++++ ++FS KA +NF L C++ I+R F + C +GV P+L LS +L F +TA+ D+ +F+++ + THEP + + F F +P++S I P G L P +++ V V F P LS IK EA + R+ E +++ K ES + ++KDQI AT N+S S+ P I+IDS+++L K SLL F V C + SD + + ++ + +LH+EL +++P L+++S SG + F V +K +KK+I IQNIS + + S++L+ NG F + NA+R + P L+++F P + + +L + L +T +A+ + G + V NE + + N S ++V + + L S + L++ +N + L +F ++P GVI+ + + V+F PDH S +R+ ++ I +RG M++ P ++ + P + E SL + L+S Y + I + +GCV + S KK E+ +NL+ + GF V P++G ++A + + ++W PP+ +++ +T+KGE
Sbjct: 93 QAKEELKAFHLHVSELEEQRDKIEEQMENEKQAGNSVPLFRLRAQLKRVCAELQAEEDLGDHLEMVLKEHELELCQVEVELGRFLDLRQELELEEKTIQSQDQKTQKLRFQREKRVIRGRRHKAHQDEIEREKLL---QEQMDLQKKQQEQALASHLKAAVYLKQTLQRIKQ-KDAEAEERKQELLKKRKTAVMALKASYAATQESINARKQRQKASEQIQREQEQQMKEWLEARGMNSTEHLHRLKLQERHSKRNEEFEEKRRARQMEIVAKLLLERN--EEKHKKAPAPIHGKKQPGKLLQDI---------LSSSEHTKREQRHEVLS-----PRASFVSSRSDSISEVSFSPEC---------VEMKDEDDGDMSLAQPEFTGLWEHKHTDYMASSDENIELETSHTTMKTKLNKSSASKAEK-----------------KTVKSRESKGPPFISKPEVILFKDWEVGRIYKKKVTLTNVTYGTNYCKLIGVSDNLRDHVSVSFEPPGPMSAGMACELEAIFRPSVDVDLDGVIHFQSAAGPFSVPIKCSRKKCEMVVDQCFVDFGTHVVGQTVSRMITMTNRGALGTRYTLTPVSSLQQTPVSGKTDVEMPSLNEEKAEITSGMKKTHKMSLTVTRALESPIRGPDEVLMADPES--VETKVMQSTIEPPEISIGQASEGEVGPFMSVRVPIIFTPTIPGEAKLDFQITFSQPECEPIDISAHGVAESVPVWVNNPNIDLKICTYDRLYQDGIQVQSRASTALRLTFKVCKEMRNHMSIQPKTGLIQAKSSFTAQLKFLPRPSLYADAKSFFNEDTGVLEVPLTVQVADQVKPVLFTAHAVVTSSDLRF---DRSE--VDFGHCSIYECVQTAVRLTNCSLLPQEFGFVGIPKFIDVQPNDGFGTLLPSQSMDIYLIFSALKAGEHNFTLTCKTSINRDFQLCCRAVGVQPSLKLSDTVLEFGDTALGDRRTAVLFVEDQRPT---QTHEPTE-----------RLFTFSVPEDSDFTIAPSSGRLLPGQRRLVQVTFSPALSDDMIKAEA---LRRRDESQSDRQQTDSKSKSESPLTVMKDQIS----------------------KATPANES-------------SFHSWSPADIQIDSSEYLSAKASLLRSFTERHVRHLVPCFTST---SDTTEEPRHSPHETLHVELRGLAVRPALLLISESGHNTLHFRTLAVGQKVVKKVI-IQNISSQTVTLSSSLLDVNGPFSVLNAMRSIAPEDKHTLLIAFT---PLLAKKYCETLEVSCSQMTLELTLLGEAVDPIITCS--HEGLLDFDWVPENESTSQVFRLQNVSSVDVQFSVLLESLCPSEQKLSSTAAVGVQNYSGLSVFGVSPAGGVISPAGAADITVTFHPDHRSLHYRDQLRVQISSMQTLCKLELRGSSCRHNMFVCGGDPVDV----SYESLIPSHIYTPGHTHHQESTSLL--LTLRSEYQEGKAIAATRK-LHIGCVYSCKTSTKKGVEFMWENLSAVEQQGFRVTPAQGSVDAGQRRPVTVTWTPPAGLAPNAVVQACAQLTVKGE 1549
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Xenopus
Match: cfap74 (cilia and flagella associated protein 74 [Source:NCBI gene;Acc:100158555]) HSP 1 Score: 546.199 bits (1406), Expect = 2.975e-165 Identity = 311/898 (34.63%), Postives = 504/898 (56.12%), Query Frame = 2
Query: 2153 IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHIS-DRSSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSSHFYGQTN------------------LTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETS 4789
I P G +I + ++P+ G F I F++ K + V+G AIDVPVY+ + + + IC +RLYQD+I+ ++RA TAL+L+F V E+K+++E+LP TGFIQA +F Q+K L R S+ + FD +TG+L+ + ++A+Q + V F+ AI+T++DL+I + +DFG CTI E ++QL NK ILPQ+FGF G PE++EIQPN G+GT+LP ET++L V+F KAK Y F+L C+S I+R F VSC IGVHP L LS ++ FP TA++D S + + N+H S N +TH P+IG G + PIGP +F+F +P++ PI I P G++ P ++ K+ V F+P LS I+ EA +V R E A E+E + ++ R E +KV + K+ + K K +LM P EL KPE I+++S+++ + S+ +F FE + C IA G S + ++ YN+L+LELHCP+I P LII S +G+ +IDFGE +R+ K +++QNIS E ++ +ILNP G F + N + +EP A L+++F+ + +F N + R L ++ K +A+T + L+ + G VL N+ + + N S + V Y ++L S + + + T+N N L +F++ P G I GK+Q L V+FSPDH S + +++ +F ++ + + G + MY++ P +D ++ M+ +D+++ +N+ ++ + ++ VGC+RT + KK+ E+ +N+ VL GFT++P+K +E KSI+I W PP+ Y+ + T+ +TLKG+ +
Sbjct: 802 IGPLGTVKIPVIFTPMVPGKSFAQFEITFDNSSCKTIPVLVTGDAIDVPVYLPNSNIDLKICTYDRLYQDSIMVHNRARTALRLKFEVCKELKNHMELLPKTGFIQAQSSFSVQLKFLPRHSLPEDAGTCFDKDTGVLEAPMTITVADQTRPVPFTVHAIVTSSDLEISPTE-----VDFGYCTIVEAVQASLQLTNKSILPQDFGFVGIPEYVEIQPNDGFGTLLPLETVKLDVIFRAEKAKEYKFELTCKSAINRQFKVSCKAIGVHPPLALSHSLIQFPATALNDASIATLHVINSHTSRNEFTHPVPRIGKGDIAPIGPTSFEFHVPEDYPITISPAVGTVLPGQRCKIQVSFQPLLSQKEIREEAIHIVRRATE--AQATMETEATQMLEKEMQGRKE-KKVMSGKKEKKPLPSPKQHPKEKG----PQLMHSVPT--ELPKPEDIKVNSDEYAAAQESIYRRFMGKFERYVIPCFIAIGDTSKSKDENFKFSHYNTLYLELHCPAIAPPLIITSENGRHLIDFGEIAIGQRVVKRVALQNISLEPVELSVSILNPCGPFMLLNPLSVVEPGANCFLLIAFLPDENKVFFENMKV--SCRHATLTLSIKGRALTPSVSCS-LEGRILDMGYVLANDSSSTTFKLQNTSTVPVTYSVKLGSLSVSRYSELQKLPPFITSLKRPSALVGTQNFNGLSVFSVHPVEGTILPGKTQDLTVNFSPDHESFHYSDVLTVELFGKLSAHTIQMMGACRNHIMYVEGGDP-----LDVAVESLSVIPMEEEDSSD----LVNSLLLTLHCIQTETALKPGVRELHVGCIRTSLLPTKKSVEFFWENMQVLQQKGFTIEPAKAAVEPGQRKSISIQWTPPAGYDPMNPARTTAKLTLKGDIT 1673 HSP 2 Score: 251.906 bits (642), Expect = 8.920e-67 Identity = 197/645 (30.54%), Postives = 342/645 (53.02%), Query Frame = 2
Query: 266 LQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKAHKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSL-SKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTID-YKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTNCSIKNMSE----IDNETRKVVSREPNSAVNIDVQTDLLDTKPITTSLD-HLKLWIEPYGEQEI 2179
+Q+ + EL C I L +E E + NIAA+ RLQ+ KR+ E++ E+ + + + + E D ++E GK E+L DE+ ER+EKER +R + EK L A + + ++ K + E + +K H +FL++T+ + R +N+ ++ E+ ++ SV L + I +ENL I ++ K++ + K+E+ +SV+ +G + + Q++ E K+K++ + +K +I +++ EE +K+ K + L + K K S + T + S +Y + + EK + W + P SSDEE + + + S + + +S ++ ++ PEF GLW YKV K ++ ++ SKM+ EIM+ L +L+ I++ Q+VS FKG F SKPN++ F+NL+V + Y++++ LTN SY+ N C+L VSE+L DF+TI+F PPG +S GM+CE + F+P +NEDL GE+K +Q+ F IPVKC+TKKC ++++++ I F T V+GE+++Q + L N GA GT SI ++ + ET V S + ++ N+ +T+ P+ D H+ E +E+
Sbjct: 114 IQKIREELTACRERIQTLKQEKNNAERETVVEQETNNIAAVSRLQAVHKRLCTELDNEEAVASTLATMLKENEFDLWQIEVEHGKFDSLRERLVKDEEQLERQEKERASQRAQDEKTLLTLAERKKQKEQLRETKRLKEQEVQYRKVMEDAQKNHVTAVQFLKETMTRIRERENKKEMKSREEMERRMQSVLSLKNSISTNRENLRVIEARTKAEAMKAKKQEILENESVLARGEDVTKFIIHQKRLKEFEKRKQEFEEQQRIQKQEIISRLLKEESMQEKQRKQVSSL--DLSKGRKSSKLRAKTMQYIKSVVYPEPE----EKKDPQGNW-RSPSPLSFSSDEETSSSVE-----EKSPDVPPRFTYSSEEEEAESLAQPEFVGLWSQNYKPYKVPKEETDMNLQ--RGSKMEMEIMAERLQKLRSGIVEKQVVSGHEFKGCPFHSKPNLVHFKNLDVGKIYKKRITLTNASYTINFCKLVGVSEHLKDFITIQFDPPGAMSAGMSCEMVVIFKPMINEDLEGEVKLLAQTGPFCIPVKCMTKKCELALEKEHIDFGTHVVGETVIQTITLINHGALGTKFSIHTLTHDSGTCEAETAHVASMKKIASPNLHKETETSLVSPVQDHSDQHMSQQTETQSREEL 744
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Mouse
Match: Cfap74 (cilia and flagella associated protein 74 [Source:MGI Symbol;Acc:MGI:1917130]) HSP 1 Score: 701.434 bits (1809), Expect = 0.000e+0 Identity = 463/1607 (28.81%), Postives = 801/1607 (49.84%), Query Frame = 2
Query: 266 LQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTN-RAVEKAHKKHS---EFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPK--TGKS--SSDEELENDFDFRNNVDNSIET----KIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGT--------------------------NCSIKNMSEIDNETRKVVSREPNSAVNIDVQT------DLLDTKPITTSLDHLKLW-----------------IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEF-------LANKK--WESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSK--IVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIEL------------------SSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKT----DDNNEDEKPSLNTPIILKSAY---SDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVT 4795
+Q+ K EL +C + LNK+ ++ EI T + N+AA+ RLQ+ +R+Q E+E EKDL+ I + +E H ++++G+ E ++ + + E ++ + + A++ E ++ A+ L TQ+ L + + VE A + H +FL+ ++ + R + + E+ ++ +V L + I +E L + ++ L +K L ++ ++ +GG+ + QR+ E+ QK K +K +I ++I+ EE +++ + R P LSK ++ L+ TW+ GKS +S +E E + I+ +++ S +D + P+ GLW+ Y+V K ++ K SKM+++I++R + QL+ ++Q Q+VS + FKG F SKP +I F++ ++ + Y++K+ L N +Y+ N C+L V ENL DF+ I F PPGP+S GM+CE + F+P +N+DL G + F +Q+ F++P+KC TKKC +S+D+++I F T V+GE+ + + L N G GT C K + E + E N + +D+Q+ L D + T ++ + + I P+ ++ I ++P+ G+ F++ F++ + R +G AIDVPV++ TV + ICM +RLYQD+I ++R+ AL+L+F V E++ ++E+LP TG+IQA + Q+K L R+S+ + YFD + +L+ + +A+Q+K V F+ QAI+TT+DL+I + ++FG CTI E I L N +LPQEFGF G P++++IQPN G+GTILP ETL L V+F P KAK Y F+L C+S I+R F VSC +GVHP L+LS + F T++ D S +++ N+HLS N H P+IG P+GP +F+F++P NSPI I P G++ P ++ + V F+P L I EA+ ++N+++E + +K W+ ++++ +D P +V P + EL +P I S +F + +L + F F + C +A+G I DR + + ++ +N+L+LEL CP++ P +++ S+ GK +FG+ R K I++QNI E + ++LNPNG F N KL LV+SF + + Q + ++ L++T + A + + N G VL E + N S L + + + L +SH + T+N N +F+I P G++ GK+Q V+FSPDH S F +++K +F++ +++G + M+++ P ++ P E++ D +++E L PI++ Y D A VGC+RT S ++ E+S+D ++ L GF+++PS+G +E K+INISW+PP++++ L S L+ L+G+ T
Sbjct: 92 IQKTKGELHICRQRMDLLNKQQESLAAEIATEKEANNMAAIGRLQAASRRLQTELENEKDLQSKITAMLKDSENAMWHIEIQKGQF----EDVRKHHEAEAEARQRGLEVHSARQLQREREAMEKSEKNRLLRARKSLHTQKELGLRHQKLVEDAQRNHRIAVKFLKASLGRVREREQKEEMESRTHMQRRMDAVLSLKNNITASRETLKKFQAWGQTRADLAKQKALTEKEVILSQGGDAFKYLFHQRRHQELEAQKRAFEEEQKLRKQEIVNRILKEEAEEEQR--------KRRQHP-------------LSKPINRRTLRD-------KTWQYISDFCEGKSVLTSQQERERELLLYPKTVCVIKAISSESVQVDLGSISTEDEVLAEPDISGLWNKE-SYQVPK--EDMERKPVGGSKMEKDILARTMEQLRSGVVQKQVVSGREFKGRPFNSKPEVIHFKDFDIGKVYKKKITLINATYTINYCKLVGVEENLKDFIHIDFEPPGPMSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGSFSVPLKCSTKKCSLSLDKELIDFGTYVVGETTSRIITLTNVGGLGTKFKFLPDSEFYEMDESQPAMKISSLFTCEEKIIYEKIMTSLSEQQLEVNDSSLVDLQSLKESEKQLDDPEVTTAAVSAMTMIPSEEQAEITLGEVTEGEIGPFSSVKVPITFTPVIPGEVQTKFKVMFKNPQSPPLYFRATGTAIDVPVWVPKATVDLKICMYDRLYQDSITVHTRSKAALRLKFEVCKELRGHIELLPETGYIQAQSTYSVQLKFLPRQSLPEDARKYFDPASRVLEAPMTIRVADQIKPVRFTVQAIVTTSDLEI-----NPSEINFGYCTIYEAIRTEICLSNLSLLPQEFGFVGLPKYVDIQPNDGFGTILPLETLHLDVIFQPIKAKEYKFELVCKSEINRCFKVSCQAVGVHPPLELSHYQIKFSATSLYDTSVSTLYVINSHLSMNKMIHSLPRIGSEEAAPVGPTSFEFLLPPNSPITISPSVGTVLPGKRCLIQVAFQPVLPKEIIYKEASQILNKEIETKPVSQKEIVQRKELWKQSFSVVRVHNRDR---PTRVST------------------------------PQATELQRP-VINSSSTEFQIAQATLSKAFQGKFNRFVIPCVVASGDIKDRKTAEPLSFSPHNTLYLELWCPAVAPFIVVTSHKGKTDFNFGDIAVGHRSVKKITLQNICNEDLTLEYSVLNPNGPFVRLNPFNKLRSGETQTLVLSFSPHENILAQETLDII--TKRGTLSLTLFGMGV-ASMITCSIDGNILNMGYVLARESVSTNFKLQNESSLPIKFWVRLESLSRKKAEAHQQLPKFITSHEQRAEIVGTQNYNGQSVFSIVPVEGLMFPGKAQEFTVTFSPDHESLFFSDLLKVVLFEKKVSHQILLKGAAREHMMFVEGGDPLDV----------PVESLAVVTAFDTEHKEEAEELK-PILVTLNYVQLDTDTTTSPATRELQVGCIRTTQPSPRRTVEFSLDGVSWLQHKGFSIEPSRGSVERGQTKTINISWVPPADFDPDHPLMVSTLLQLRGDVKET 1610
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Mouse
Match: Cfap74 (cilia and flagella associated protein 74 [Source:MGI Symbol;Acc:MGI:1917130]) HSP 1 Score: 694.501 bits (1791), Expect = 0.000e+0 Identity = 457/1588 (28.78%), Postives = 792/1588 (49.87%), Query Frame = 2
Query: 266 LQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTN-RAVEKAHKKHS---EFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEE-DNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPK--TGKS--SSDEELENDFDFRNNVDNSIET----KIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGT--------------------------NCSIKNMSEIDNETRKVVSREPNSAVNIDVQT------DLLDTKPITTSLDHLKLW-----------------IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEF-------LANKK--WESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSK--IVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIEL------------------SSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKT----DDNNEDEKPSLNTPIILKSAY---SDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYE 4735
+Q+ K EL +C + LNK+ ++ EI T + N+AA+ RLQ+ +R+Q E+E EKDL+ I + +E H ++++G+ E ++ + + E ++ + + A++ E ++ A+ L TQ+ L + + VE A + H +FL+ ++ + R + + E+ ++ +V L + I +E L + ++ L +K L ++ ++ +GG+ + QR+ E+ QK K +K +I ++I+ EE + ++K + P LSK ++ L+ TW+ GKS +S +E E + I+ +++ S +D + P+ GLW+ Y+V K ++ K SKM+++I++R + QL+ ++Q Q+VS + FKG F SKP +I F++ ++ + Y++K+ L N +Y+ N C+L V ENL DF+ I F PPGP+S GM+CE + F+P +N+DL G + F +Q+ F++P+KC TKKC +S+D+++I F T V+GE+ + + L N G GT C K + E + E N + +D+Q+ L D + T ++ + + I P+ ++ I ++P+ G+ F++ F++ + R +G AIDVPV++ TV + ICM +RLYQD+I ++R+ AL+L+F V E++ ++E+LP TG+IQA + Q+K L R+S+ + YFD + +L+ + +A+Q+K V F+ QAI+TT+DL+I + ++FG CTI E I L N +LPQEFGF G P++++IQPN G+GTILP ETL L V+F P KAK Y F+L C+S I+R F VSC +GVHP L+LS + F T++ D S +++ N+HLS N H P+IG P+GP +F+F++P NSPI I P G++ P ++ + V F+P L I EA+ ++N+++E + +K W+ ++++ +D P +V P + EL +P I S +F + +L + F F + C +A+G I DR + + ++ +N+L+LEL CP++ P +++ S+ GK +FG+ R K I++QNI E + ++LNPNG F N KL LV+SF + + Q + ++ L++T + A + + N G VL E + N S L + + + L +SH + T+N N +F+I P G++ GK+Q V+FSPDH S F +++K +F++ +++G + M+++ P ++ P E++ D +++E L PI++ Y D A VGC+RT S ++ E+S+D ++ L GF+++PS+G +E K+INISW+PP++++
Sbjct: 92 IQKTKGELHICRQRMDLLNKQQESLAAEIATEKEANNMAAIGRLQAASRRLQTELENEKDLQSKITAMLKDSENAMWHIEIQKGQF----EDVRKHHEAEAEARQRGLEVHSARQLQREREAMEKSEKNRLLRARKSLHTQKELGLRHQKLVEDAQRNHRIAVKFLKASLGRVREREQKEEMESRTHMQRRMDAVLSLKNNITASRETLKKFQAWGQTRADLAKQKALTEKEVILSQGGDAFKYLFHQRRHQELEAQKRAFEEEQKLRKQEIVNRILKEEAEEEQRKRRQHP----------------------LSKPINRRTLRD-------KTWQYISDFCEGKSVLTSQQERERELLLYPKTVCVIKAISSESVQVDLGSISTEDEVLAEPDISGLWNKE-SYQVPK--EDMERKPVGGSKMEKDILARTMEQLRSGVVQKQVVSGREFKGRPFNSKPEVIHFKDFDIGKVYKKKITLINATYTINYCKLVGVEENLKDFIHIDFEPPGPMSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGSFSVPLKCSTKKCSLSLDKELIDFGTYVVGETTSRIITLTNVGGLGTKFKFLPDSEFYEMDESQPAMKISSLFTCEEKIIYEKIMTSLSEQQLEVNDSSLVDLQSLKESEKQLDDPEVTTAAVSAMTMIPSEEQAEITLGEVTEGEIGPFSSVKVPITFTPVIPGEVQTKFKVMFKNPQSPPLYFRATGTAIDVPVWVPKATVDLKICMYDRLYQDSITVHTRSKAALRLKFEVCKELRGHIELLPETGYIQAQSTYSVQLKFLPRQSLPEDARKYFDPASRVLEAPMTIRVADQIKPVRFTVQAIVTTSDLEI-----NPSEINFGYCTIYEAIRTEICLSNLSLLPQEFGFVGLPKYVDIQPNDGFGTILPLETLHLDVIFQPIKAKEYKFELVCKSEINRCFKVSCQAVGVHPPLELSHYQIKFSATSLYDTSVSTLYVINSHLSMNKMIHSLPRIGSEEAAPVGPTSFEFLLPPNSPITISPSVGTVLPGKRCLIQVAFQPVLPKEIIYKEASQILNKEIETKPVSQKEIVQRKELWKQSFSVVRVHNRDR---PTRVST------------------------------PQATELQRP-VINSSSTEFQIAQATLSKAFQGKFNRFVIPCVVASGDIKDRKTAEPLSFSPHNTLYLELWCPAVAPFIVVTSHKGKTDFNFGDIAVGHRSVKKITLQNICNEDLTLEYSVLNPNGPFVRLNPFNKLRSGETQTLVLSFSPHENILAQETLDII--TKRGTLSLTLFGMGV-ASMITCSIDGNILNMGYVLARESVSTNFKLQNESSLPIKFWVRLESLSRKKAEAHQQLPKFITSHEQRAEIVGTQNYNGQSVFSIVPVEGLMFPGKAQEFTVTFSPDHESLFFSDLLKVVLFEKKVSHQILLKGAAREHMMFVEGGDPLDV----------PVESLAVVTAFDTEHKEEAEELK-PILVTLNYVQLDTDTTTSPATRELQVGCIRTTQPSPRRTVEFSLDGVSWLQHKGFSIEPSRGSVERGQTKTINISWVPPADFD 1590
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Mouse
Match: Cfap74 (cilia and flagella associated protein 74 [Source:MGI Symbol;Acc:MGI:1917130]) HSP 1 Score: 648.277 bits (1671), Expect = 0.000e+0 Identity = 439/1542 (28.47%), Postives = 759/1542 (49.22%), Query Frame = 2
Query: 266 LQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTN-RAVEKAHKKHS---EFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPK--TGKS--SSDEELENDFDFRNNVDNSIET----KIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGT--------------------------NCSIKNMSEIDNETRKVVSREPNSAVNIDVQT------DLLDTKPITTSLDHLKLW-----------------IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEF-------LANKK--WESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSK--IVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIEL------------------SSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKT----DDNNEDEKPSLNTPIILKSAY---SDDGLIQEAKFCFTVGCVRTLAVSQKK 4600
+Q+ K EL +C + LNK+ ++ EI T + N+AA+ RLQ+ +R+Q E+E EKDL+ I + +E H ++++G+ E ++ + + E ++ + + A++ E ++ A+ L TQ+ L + + VE A + H +FL+ ++ + R + + E+ ++ +V L + I +E L + ++ L +K L ++ ++ +GG+ + QR+ E+ QK K +K +I ++I+ EE +++ + R P LSK ++ L+ TW+ GKS +S +E E + I+ +++ S +D + P+ GLW+ Y+V K ++ K SKM+++I++R + QL+ ++Q Q+VS + FKG F SKP +I F++ ++ + Y++K+ L N +Y+ N C+L V ENL DF+ I F PPGP+S GM+CE + F+P +N+DL G + F +Q+ F++P+KC TKKC +S+D+++I F T V+GE+ + + L N G GT C K + E + E N + +D+Q+ L D + T ++ + + I P+ ++ I ++P+ G+ F++ F++ + R +G AIDVPV++ TV + ICM +RLYQD+I ++R+ AL+L+F V E++ ++E+LP TG+IQA + Q+K L R+S+ + YFD + +L+ + +A+Q+K V F+ QAI+TT+DL+I + ++FG CTI E I L N +LPQEFGF G P++++IQPN G+GTILP ETL L V+F P KAK Y F+L C+S I+R F VSC +GVHP L+LS + F T++ D S +++ N+HLS N H P+IG P+GP +F+F++P NSPI I P G++ P ++ + V F+P L I EA+ ++N+++E + +K W+ ++++ +D P +V P + EL +P I S +F + +L + F F + C +A+G I DR + + ++ +N+L+LEL CP++ P +++ S+ GK +FG+ R K I++QNI E + ++LNPNG F N KL LV+SF + + Q + ++ L++T + A + + N G VL E + N S L + + + L +SH + T+N N +F+I P G++ GK+Q V+FSPDH S F +++K +F++ +++G + M+++ P ++ P E++ D +++E L PI++ Y D A VGC+RT S ++
Sbjct: 92 IQKTKGELHICRQRMDLLNKQQESLAAEIATEKEANNMAAIGRLQAASRRLQTELENEKDLQSKITAMLKDSENAMWHIEIQKGQF----EDVRKHHEAEAEARQRGLEVHSARQLQREREAMEKSEKNRLLRARKSLHTQKELGLRHQKLVEDAQRNHRIAVKFLKASLGRVREREQKEEMESRTHMQRRMDAVLSLKNNITASRETLKKFQAWGQTRADLAKQKALTEKEVILSQGGDAFKYLFHQRRHQELEAQKRAFEEEQKLRKQEIVNRILKEEAEEEQR--------KRRQHP-------------LSKPINRRTLRD-------KTWQYISDFCEGKSVLTSQQERERELLLYPKTVCVIKAISSESVQVDLGSISTEDEVLAEPDISGLWNKE-SYQVPK--EDMERKPVGGSKMEKDILARTMEQLRSGVVQKQVVSGREFKGRPFNSKPEVIHFKDFDIGKVYKKKITLINATYTINYCKLVGVEENLKDFIHIDFEPPGPMSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGSFSVPLKCSTKKCSLSLDKELIDFGTYVVGETTSRIITLTNVGGLGTKFKFLPDSEFYEMDESQPAMKISSLFTCEEKIIYEKIMTSLSEQQLEVNDSSLVDLQSLKESEKQLDDPEVTTAAVSAMTMIPSEEQAEITLGEVTEGEIGPFSSVKVPITFTPVIPGEVQTKFKVMFKNPQSPPLYFRATGTAIDVPVWVPKATVDLKICMYDRLYQDSITVHTRSKAALRLKFEVCKELRGHIELLPETGYIQAQSTYSVQLKFLPRQSLPEDARKYFDPASRVLEAPMTIRVADQIKPVRFTVQAIVTTSDLEI-----NPSEINFGYCTIYEAIRTEICLSNLSLLPQEFGFVGLPKYVDIQPNDGFGTILPLETLHLDVIFQPIKAKEYKFELVCKSEINRCFKVSCQAVGVHPPLELSHYQIKFSATSLYDTSVSTLYVINSHLSMNKMIHSLPRIGSEEAAPVGPTSFEFLLPPNSPITISPSVGTVLPGKRCLIQVAFQPVLPKEIIYKEASQILNKEIETKPVSQKEIVQRKELWKQSFSVVRVHNRDR---PTRVST------------------------------PQATELQRP-VINSSSTEFQIAQATLSKAFQGKFNRFVIPCVVASGDIKDRKTAEPLSFSPHNTLYLELWCPAVAPFIVVTSHKGKTDFNFGDIAVGHRSVKKITLQNICNEDLTLEYSVLNPNGPFVRLNPFNKLRSGETQTLVLSFSPHENILAQETLDII--TKRGTLSLTLFGMGV-ASMITCSIDGNILNMGYVLARESVSTNFKLQNESSLPIKFWVRLESLSRKKAEAHQQLPKFITSHEQRAEIVGTQNYNGQSVFSIVPVEGLMFPGKAQEFTVTFSPDHESLFFSDLLKVVLFEKKVSHQILLKGAAREHMMFVEGGDPLDV----------PVESLAVVTAFDTEHKEEAEELK-PILVTLNYVQLDTDTTTSPATRELQVGCIRTTQPSPRR 1545
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Mouse
Match: Cfap74 (cilia and flagella associated protein 74 [Source:MGI Symbol;Acc:MGI:1917130]) HSP 1 Score: 59.6918 bits (143), Expect = 6.077e-9 Identity = 50/211 (23.70%), Postives = 88/211 (41.71%), Query Frame = 2
Query: 4043 FNCGSVLINEFNERPLTITNNSKLNVDYRIEL------------------SSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKT----DDNNEDEKPSLNTPIILKSAY---SDDGLIQEAKFCFTVGCVRTLAVSQKK 4600
N G VL E + N S L + + + L +SH + T+N N +F+I P G++ GK+Q V+FSPDH S F +++K +F++ +++G + M+++ P ++ P E++ D +++E L PI++ Y D A VGC+RT S ++
Sbjct: 16 LNMGYVLARESVSTNFKLQNESSLPIKFWVRLESLSRKKAEAHQQLPKFITSHEQRAEIVGTQNYNGQSVFSIVPVEGLMFPGKAQEFTVTFSPDHESLFFSDLLKVVLFEKKVSHQILLKGAAREHMMFVEGGDPLDV----------PVESLAVVTAFDTEHKEEAEELK-PILVTLNYVQLDTDTTTSPATRELQVGCIRTTQPSPRR 215
BLAST of Cilia-and flagella-associated protein 74 vs. UniProt/SwissProt
Match: sp|Q3UY96|CFA74_MOUSE (Cilia- and flagella-associated protein 74 OS=Mus musculus OX=10090 GN=Cfap74 PE=2 SV=3) HSP 1 Score: 648.277 bits (1671), Expect = 0.000e+0 Identity = 439/1542 (28.47%), Postives = 759/1542 (49.22%), Query Frame = 2
Query: 266 LQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTN-RAVEKAHKKHS---EFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPK--TGKS--SSDEELENDFDFRNNVDNSIET----KIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGT--------------------------NCSIKNMSEIDNETRKVVSREPNSAVNIDVQT------DLLDTKPITTSLDHLKLW-----------------IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEF-------LANKK--WESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSK--IVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIEL------------------SSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKT----DDNNEDEKPSLNTPIILKSAY---SDDGLIQEAKFCFTVGCVRTLAVSQKK 4600
+Q+ K EL +C + LNK+ ++ EI T + N+AA+ RLQ+ +R+Q E+E EKDL+ I + +E H ++++G+ E ++ + + E ++ + + A++ E ++ A+ L TQ+ L + + VE A + H +FL+ ++ + R + + E+ ++ +V L + I +E L + ++ L +K L ++ ++ +GG+ + QR+ E+ QK K +K +I ++I+ EE +++ + R P LSK ++ L+ TW+ GKS +S +E E + I+ +++ S +D + P+ GLW+ Y+V K ++ K SKM+++I++R + QL+ ++Q Q+VS + FKG F SKP +I F++ ++ + Y++K+ L N +Y+ N C+L V ENL DF+ I F PPGP+S GM+CE + F+P +N+DL G + F +Q+ F++P+KC TKKC +S+D+++I F T V+GE+ + + L N G GT C K + E + E N + +D+Q+ L D + T ++ + + I P+ ++ I ++P+ G+ F++ F++ + R +G AIDVPV++ TV + ICM +RLYQD+I ++R+ AL+L+F V E++ ++E+LP TG+IQA + Q+K L R+S+ + YFD + +L+ + +A+Q+K V F+ QAI+TT+DL+I + ++FG CTI E I L N +LPQEFGF G P++++IQPN G+GTILP ETL L V+F P KAK Y F+L C+S I+R F VSC +GVHP L+LS + F T++ D S +++ N+HLS N H P+IG P+GP +F+F++P NSPI I P G++ P ++ + V F+P L I EA+ ++N+++E + +K W+ ++++ +D P +V P + EL +P I S +F + +L + F F + C +A+G I DR + + ++ +N+L+LEL CP++ P +++ S+ GK +FG+ R K I++QNI E + ++LNPNG F N KL LV+SF + + Q + ++ L++T + A + + N G VL E + N S L + + + L +SH + T+N N +F+I P G++ GK+Q V+FSPDH S F +++K +F++ +++G + M+++ P ++ P E++ D +++E L PI++ Y D A VGC+RT S ++
Sbjct: 92 IQKTKGELHICRQRMDLLNKQQESLAAEIATEKEANNMAAIGRLQAASRRLQTELENEKDLQSKITAMLKDSENAMWHIEIQKGQF----EDVRKHHEAEAEARQRGLEVHSARQLQREREAMEKSEKNRLLRARKSLHTQKELGLRHQKLVEDAQRNHRIAVKFLKASLGRVREREQKEEMESRTHMQRRMDAVLSLKNNITASRETLKKFQAWGQTRADLAKQKALTEKEVILSQGGDAFKYLFHQRRHQELEAQKRAFEEEQKLRKQEIVNRILKEEAEEEQR--------KRRQHP-------------LSKPINRRTLRD-------KTWQYISDFCEGKSVLTSQQERERELLLYPKTVCVIKAISSESVQVDLGSISTEDEVLAEPDISGLWNKE-SYQVPK--EDMERKPVGGSKMEKDILARTMEQLRSGVVQKQVVSGREFKGRPFNSKPEVIHFKDFDIGKVYKKKITLINATYTINYCKLVGVEENLKDFIHIDFEPPGPMSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGSFSVPLKCSTKKCSLSLDKELIDFGTYVVGETTSRIITLTNVGGLGTKFKFLPDSEFYEMDESQPAMKISSLFTCEEKIIYEKIMTSLSEQQLEVNDSSLVDLQSLKESEKQLDDPEVTTAAVSAMTMIPSEEQAEITLGEVTEGEIGPFSSVKVPITFTPVIPGEVQTKFKVMFKNPQSPPLYFRATGTAIDVPVWVPKATVDLKICMYDRLYQDSITVHTRSKAALRLKFEVCKELRGHIELLPETGYIQAQSTYSVQLKFLPRQSLPEDARKYFDPASRVLEAPMTIRVADQIKPVRFTVQAIVTTSDLEI-----NPSEINFGYCTIYEAIRTEICLSNLSLLPQEFGFVGLPKYVDIQPNDGFGTILPLETLHLDVIFQPIKAKEYKFELVCKSEINRCFKVSCQAVGVHPPLELSHYQIKFSATSLYDTSVSTLYVINSHLSMNKMIHSLPRIGSEEAAPVGPTSFEFLLPPNSPITISPSVGTVLPGKRCLIQVAFQPVLPKEIIYKEASQILNKEIETKPVSQKEIVQRKELWKQSFSVVRVHNRDR---PTRVST------------------------------PQATELQRP-VINSSSTEFQIAQATLSKAFQGKFNRFVIPCVVASGDIKDRKTAEPLSFSPHNTLYLELWCPAVAPFIVVTSHKGKTDFNFGDIAVGHRSVKKITLQNICNEDLTLEYSVLNPNGPFVRLNPFNKLRSGETQTLVLSFSPHENILAQETLDII--TKRGTLSLTLFGMGV-ASMITCSIDGNILNMGYVLARESVSTNFKLQNESSLPIKFWVRLESLSRKKAEAHQQLPKFITSHEQRAEIVGTQNYNGQSVFSIVPVEGLMFPGKAQEFTVTFSPDHESLFFSDLLKVVLFEKKVSHQILLKGAAREHMMFVEGGDPLDV----------PVESLAVVTAFDTEHKEEAEELK-PILVTLNYVQLDTDTTTSPATRELQVGCIRTTQPSPRR 1545
BLAST of Cilia-and flagella-associated protein 74 vs. UniProt/SwissProt
Match: sp|Q9C0B2|CFA74_HUMAN (Cilia- and flagella-associated protein 74 OS=Homo sapiens OX=9606 GN=CFAP74 PE=2 SV=3) HSP 1 Score: 442.965 bits (1138), Expect = 1.799e-128 Identity = 270/851 (31.73%), Postives = 435/851 (51.12%), Query Frame = 2
Query: 2153 IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDS-YELVKPESIEI-------DSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDR--SSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIEL----SSHFYGQTNL--------------TTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKT----DDNNEDEKPSLNTPIILKSAY---SDDGLIQEAKFCFTVGCVRTLAVSQKK 4600
I P+ ++ I ++P+ GD F++ F++ RV G AIDVPV++ +V + ICM +RLYQD+++ ++R+ AL+L+F V E++ +LE+LP TG+IQA ++ Q+K L R S+ + YFD ET +L+ + +A+Q K V F+ AI+TT+DL++ + +DFG CTI E I L N +LPQEFGF P+F+++QPN G+GTILP ETL+ V+F P KA+ + FQL C+S I+R F +SC +GVHP L+LS + F TA+ D S +++ N+HLS + TH P+IG P+GP +F+F++P +SPI I P G++ P ++ V V F P L I+ EA L+N+++E + +K N + K+ G S L A D +++ P +E+ S+++ + +LL F F+ V C +A+G I DR S + ++ +N+L+LEL CP++ P +++ S+ GK I +FG+ R K ISIQN+S E + ++LNPNG F + N L LV+SF + + Q + ++ L +T + A + ++ N G V+ E + NNS L + + + L S+ GQ L T+N N +F++ P +GV+ GK+Q V+FSPDH S F + ++ +F++ +++G M+++ P ++ P E++ D + +E L PI++ Y D A VGC+RT S KK
Sbjct: 753 IGPFSSIKVPIVFTPVVPGDVQARFKVTFKNPQCPTLHFRVVGVAIDVPVWVPKPSVDLKICMYDRLYQDSVLVHTRSKAALRLKFEVCKELRAHLELLPKTGYIQAQSSYSVQLKFLPRHSLPEDAGRYFDKETRVLEAPMTIWVADQNKPVGFTVHAIVTTSDLEL-----SPSEVDFGYCTIYEAIRTEISLHNHSLLPQEFGFVRLPKFVDVQPNDGFGTILPLETLQFCVIFQPTKAEEHRFQLTCKSEINRCFKLSCRAVGVHPPLELSHYQIKFAATALYDTSVATVYVINSHLSMSSPTHSKPRIGSEDASPMGPTSFEFLLPPDSPITISPSVGTVWPGKRCLVQVAFRPVLPEKLIRQEALPLLNKEMETKSFRK---------------------------------NMAPQRKDLHGLSFSVLRAQNRDKLFKVSVPHVLEMRKRELRPSSDEYQAARATLLRAFQAKFDTFVVPCVVASGDIKDRKGSEPLSFSPHNTLYLELWCPTVAPSVVVTSHKGKTIFNFGDVAVGHRSIKKISIQNVSPEDLALDFSLLNPNGPFVLLNHSSLLRAGGTQVLVLSFSPHESILAQETLDII--TKRGTLTLTLMGTGV-ASMITCSIEGSVLNMGYVIAGESVSSGFKLQNNSLLPIKFSMHLDSLSSTRGRGQQQLPQFLSSPSQRTEVVGTQNLNGQSVFSVAPVKGVMDPGKTQDFTVTFSPDHESLYFSDKLQVVLFEKKISHQILLKGAACQHMMFVEGGDPLDV----------PVESLTAIPVFDPRHREEAEELR-PILVTLDYIQFDTDTPAPPATRELQVGCIRTTQPSPKK 1551 HSP 2 Score: 206.453 bits (524), Expect = 4.789e-52 Identity = 155/592 (26.18%), Postives = 297/592 (50.17%), Query Frame = 2
Query: 269 QQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTN-RAVEKAHKKHS---EFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTAN-NLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTNCSIKNMSE 2029
++ + EL+ C ++K+ + EI T N+AA+ RLQ+ +R+ E+E E+DL+ + ++E H +++EG+L E +T ++ R + R ++ + AL +E ++ + L TQ+ L + + +E A K H FL+ ++ + R + + E E ++ +V L I ++ L + ++ L ++ +K+++ +G + + QR++ E+ QK K +K +I +I+ EE +K+ K P P+ L + + + + + D + T+ +E G S E + ++ I+ ++ +++T + PE GLW+ DYK + ++ K +KMD++I+ R + +L+ ++ Q+V + F+G F SKP ++ F++ ++ + Y++K+ L NT+Y+ N C+L V E+L DF+ + F PPGPLS GM+CE + F+P +N+DL G I F +Q+ +F++P+KC TKKC +S+D+++I F + V+GE+ + + L N G GT SE
Sbjct: 96 EKMRGELRACRQRRDLIDKQQEAVAAEIATEEEAGNMAAVGRLQAVSRRLFAELENERDLQSRTEAVLKESENTMWHIEIQEGRL----EAFRTADREEVEATGRRLQVRAAEQLCREQEALGKVERNRLLRIRKSLNTQKELGLRHQKLLEDARKNHKVAVRFLKASLGRIREQEKKEEMECHEYMRRRMDAVVALKGSISANRDTLRKFQAWDRAKAELAEQRVQAEKKAILAQGRDAFRHLVHQRRRQELEAQKRAFEEEQKLRKQEIISRILKEEAEEEKRKKQHP--------PTSARHRLTLRDKTWNYISDFCKKTTVPTNTYTLDYEAAAGPGPSRLLEVVSSEL-------------IQGDPGASSEEET-LAEPEISGLWNE--DYKPYQVPKEDVDRKPVGGTKMDKDILERTVERLRSRVVHKQVVWGREFQGRPFNSKPELLHFQDFDIGKVYKKKITLVNTTYTINYCKLVGVEEHLRDFIHVDFDPPGPLSAGMSCEVLVTFKPMINKDLEGNISFLAQTGEFSVPLKCSTKKCSLSLDKELIDFGSYVVGETTSRTITLTNVGGLGTTFKFLPASE 659
BLAST of Cilia-and flagella-associated protein 74 vs. UniProt/SwissProt
Match: sp|D4P3R7|CFA74_CHLRE (Cilia- and flagella-associated protein 74 OS=Chlamydomonas reinhardtii OX=3055 GN=CFAP74 PE=1 SV=1) HSP 1 Score: 222.631 bits (566), Expect = 6.474e-57 Identity = 158/642 (24.61%), Postives = 271/642 (42.21%), Query Frame = 2
Query: 1475 IKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSV-IGESLVQNVKLKNSGA----------------KGTNCSIKNMSEIDNETRKVV----------SREPNSA---VNIDVQTDLL---DTKPITTSLDHLKL------------WIEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDL-----RVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIET---GILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSI 3241
+ ++ +K +Q +M + K I PQ + F G +F P ++ F++ +V V + N SY N R+ + D + + PG L+PG++ E I F PK D+ ++ + + F +P++C TK+ +S+ + F V +GE + + N GA + S NM+ + + P SA N+ V DT P+ + +L ++ Y + + ++P+ A ++ K L ++G DVPVY+E + CML LY+D +V + +A+K+ E++ + E P GF+QAG +F I+ ++L C +E IL++ ++ S+ +Q V F+ +A +T TDL P LDFG C + E + + N LPQ FGF G P + PN G+G +LP E LE V F PP A F + ++L R+FT+ C GV P + LS+ + P T I D + + + N T + PQ+++F +P +S + + P G + P + +V + + P +
Sbjct: 568 LATRALTKHEQGLMEAAKTRHKGGIGAPQTQMGRTFGGDAFLPSPAVVEFKDFDVGRTVSASVQIINRSYKKNTFRVTGIPVEYCDVLDFAYKLPGYLAPGVSGEVVISFTPKAPVDIDTSLQLLADTGPFEVPIRCRTKRAALSVSPSAVDFGAGVTLGECATRTFTIANDGALEVEFRLDSPQLSEDERALLKSTANMAHLMTSATVAAAAAAAAATASGQGPRSARSFANMGVADGAAPETDTGPLVAPREERRLAHGGFTVFPCVGHLKGYSKVTFTVTFAPVVAAPAKLLLHASYKAPAMKRLALPHHEVTLTGTGRDVPVYVERPLIDFQCCMLGHLYRDVLVVRNGGKSAMKVMVVPRPELEGFFEFSPDFGFVQAGDSFPITIRFKPTPALL-HACRKHVVEPEEEQILEIGMRLSVPDQSLPVPFTLRAQITNTDLLFQPP-----ALDFGDCVMGERTAVKLLVRNPGRLPQTFGFVGLPSGLHFTPNDGFGYVLPGEALERLVSFQPPIAGPQTFSITAKTLAGRSFTLPCRANGVAPPVVLSSNRVRMPATPIGDTRTVSVVLINK-------TDQ-------------PQSYEFAVPADSDLTLSPHVGRVPPQSRLRVQIDYSPRPPV 1183 HSP 2 Score: 78.1814 bits (191), Expect = 6.124e-13 Identity = 59/259 (22.78%), Postives = 118/259 (45.56%), Query Frame = 2
Query: 3656 LHLELHCPSIKPDLIILSNSGKPII--------DFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEF--KELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLK-IDSPE 4399
+HL++ ++ PDL++L+ P + DFG +R+ + + + N +E + + ++ VF + NA+R + P A ++VSF + + L ++ + L + K I +L+ + + + G VL+ E R LT+TN + + + L+ G+ + N F+ P G +A G+S +AVSF+P F ++++ + +Q + +RG G +++ D PE
Sbjct: 1422 IHLQVATCAVLPDLVLLAPPDLPYVPLHQCHCLDFGPVPVGQRVVRRLELANEGEEPLVLGAESMDSREVFGLVNALRPVRPGASFRVMVSFTPQARTEYLEVLVL--KSARTRLRLALKGAGIAPELQLGPEGVTATGLDMGDVLVGEAAARTLTVTNVCPFPLSFTMRLA----GKASAADPNPTMRQAFSCHPAEGTLAQGESCEVAVSFTPFSQRPYFEDVLQVVVPNQQDQLLVPLRGRGWREAVFVAGPDYPE 1674
BLAST of Cilia-and flagella-associated protein 74 vs. TrEMBL
Match: W5MG58 (Calcium channel, voltage-dependent, L type, alpha 1D subunit, b OS=Lepisosteus oculatus OX=7918 PE=4 SV=1) HSP 1 Score: 736.102 bits (1899), Expect = 0.000e+0 Identity = 498/1581 (31.50%), Postives = 827/1581 (52.31%), Query Frame = 2
Query: 278 KNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTNR-AVEKA---HKKHSEFLRKTVEKERAVKNQIAQETAEKEM-------KKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTN--------VITPEFQGLWDTTI-DYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTN---------CSIKNMSE-IDNETRKV----------VSREPNSAVNID--------------VQTDLLDTKPITTSLDHLKLW------IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSS--KIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIE---------------LSSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETS 4789
+ ELK C I+EL K+ +IE + +EN AALFRL++ KR+ E++GE+DL I + + E + ++E G+ L+ + + E+ + +QE++ + + + A ++ E R K + + + E +R AV +A HKK +L++T+ N+I Q+ AE+EM ++ +V L + I +ENL A+ ++ K+ + +KE +R+SV +GG+ + Q++ E+ ++K K +K++I KI+ EE +K+ K Q F + K DK T + ++ + +DEE + S +T + S +DD+ + PEF GLW+ +YKV N +K KMDQEI++R L + + I++ Q+V + FKG F SKP +I F++ +V + Y+++V LTN SYS N C+L VSE+L DF+++ F PPG +S GM+C+ + F+P +N+DL G+++F SQ+ F++ +KC KKC +++D +I F T V+GE++ + + L N G GT+ ++ +SE E K+ V R ++ I+ +T + P+ D L ++ I P ++ I ++P GD F I+F D + + V G AID+PV++ V + ICM +RLYQD+IV +SR++TAL+L F V E++++LEILP TGFIQA F Q+K L R S+ + FD ETG+L+V + +A+Q + V F+ A++TT+DL+ KE LDFG C+I E+ ++ L N +LPQ+FGF G P+FI++QPN G+GT+LP ETLE+ ++FSP KA+ Y+F+L C+S I+R F +SC +GVHP L+LS ++ F TA++D S +++ N+H S+N +TH P+IG G + P+GP +F+FI PDNS I I P+ G++ P ++ V V F P++S +I+ EA + K+ +SE+ K Q P AK+ +K S S + K S +E P I +S F K SLL F F+ V C +A+G +D + + Y+ +N+L+LELHCP ++P L+++S++G+ ++F E +++ + ++IQNIS++ + S++L+PNG F + NA+R LEP A+ L++SF + + + +K L + + + ++ G + G VL E + + N S L V Y ++ L+S Q + T+N + L +F+++P G I+ GKSQ + V+F PDH S + + +K + ++ ++G + TMY+ P ++ P E++ +E+ L T LKS YS + + A VGC+R+ KKN E+++DNL VL GF+VDP KG +E +K+I ++W P S + L S+ +T+KG+ +
Sbjct: 104 REELKACRLRIAELGKQRDVVERDIEELKESENTAALFRLRALHKRLCSELQGEEDLESQIEMALTQNELELCQVEVELGRF----SHLRQEVQREEQALEVQQEQQAARRLRQEETAARAAELRHRLEKKRQMNSLKEREAQHRKAVVEAQQNHKKAVLYLKETM-------NRIRQKEAEEEMQSREQMERRVQAVLSLKTNIAATRENLHALQARDKARIARQKEKERLVRESVRAEGGDVTKYMHQQKQLRELERKKRAFEEQQKSRKIQIISKILQEEALLEKRKKYQAQHF-----------------PTYRKSLDKASYSTKTREKLLEYLD----LTYGEADEESYSPV--------SRKTYLMKCNGSPERDDSEEERTTEESLAVPEFTGLWEQKHKEYKVQAALN---VKMH---KMDQEILARTLEKQRSGIVRKQVVVGREFKGCPFYSKPEVIHFKDFDVGKTYKKRVTLTNVSYSINFCKLLGVSEHLKDFISVHFEPPGAMSAGMSCDMLVTFKPMINKDLDGKVQFLSQTGPFSVSLKCTIKKCELAVDNSLIDFGTHVVGETISRVITLTNRGTLGTHFILVPSSDSGPVQLVSEPFSMEPEKIRSLVTTTISPVHRWIHTVFRIEKKLVRKMSNLFLKITKTQGTEAFPVIFLCDVLSIFQVQEGEIGPLSCIKLQIIFTPTIPGDVQMAFDIRFSDPSAQPIPITVRGVAIDLPVWVAQPNVDLKICMYDRLYQDSIVVHSRSSTALRLTFVVCKELRNHLEILPKTGFIQAQSTFNVQLKFLPRHSLPDDTGACFDKETGVLEVPLMIQVADQARPVPFTVHAVVTTSDLEF---GRKE--LDFGHCSIYESVRTSVLLTNHSLLPQDFGFVGIPKFIDVQPNDGFGTLLPLETLEIDIIFSPTKAREYSFELTCKSGINRDFKLSCRAVGVHPPLELSHSLVQFGATAVNDMSTATLYVVNSHTSSNEFTHPVPRIGRGEIAPVGPTSFEFINPDNSDITITPEVGTVMPGKRCLVQVSFRPSISDEAIREEAVR---------SKKEAQSELKRGKKQ-------PNPASAKTAVKEKCSKVSVSPKICS-------------PFEPPNPRDIHPESETFAAAKASLLRSFADCFKSYTVPCFVASGETTDGKNPEHLQYSPHNTLYLELHCPIVRPPLVVVSDNGRTSVNFNEVATGQKVVRRVTIQNISQDSLDLSSSLLDPNGPFLLLNALRHLEPGAMHTLLISFSPAQGKKYYETLQI--QTKKMTLRLNLCGIGLQPTINCS-VEGGLISFGYVLEKESATQMFKLHNTSSLLVHYSLKLESLSNFTHEGLPPFLASGLQSQAPVGTQNYSGLSVFSVSPIEGAISPGKSQDITVTFQPDHESLYYSDRLKVELMNKQTVQEVQLKGAARRHTMYVSGGDPLDV----------PVESLAFLPPDEESTAVLLT---LKSVYS-ESTVPPAVRELEVGCIRSTQPMVKKNVEFALDNLPVLQQKGFSVDPVKGTVEPGQKKTITVTWAPHSGQNLTQPLCVSVPLTVKGDVT 1587
BLAST of Cilia-and flagella-associated protein 74 vs. TrEMBL
Match: A0A571BG73 (Cilia- and flagella-associated protein 74 OS=Mus musculus OX=10090 GN=Cfap74 PE=4 SV=1) HSP 1 Score: 701.434 bits (1809), Expect = 0.000e+0 Identity = 463/1607 (28.81%), Postives = 801/1607 (49.84%), Query Frame = 2
Query: 266 LQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTN-RAVEKAHKKHS---EFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPK--TGKS--SSDEELENDFDFRNNVDNSIET----KIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGT--------------------------NCSIKNMSEIDNETRKVVSREPNSAVNIDVQT------DLLDTKPITTSLDHLKLW-----------------IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEF-------LANKK--WESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSK--IVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIEL------------------SSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKT----DDNNEDEKPSLNTPIILKSAY---SDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVT 4795
+Q+ K EL +C + LNK+ ++ EI T + N+AA+ RLQ+ +R+Q E+E EKDL+ I + +E H ++++G+ E ++ + + E ++ + + A++ E ++ A+ L TQ+ L + + VE A + H +FL+ ++ + R + + E+ ++ +V L + I +E L + ++ L +K L ++ ++ +GG+ + QR+ E+ QK K +K +I ++I+ EE +++ + R P LSK ++ L+ TW+ GKS +S +E E + I+ +++ S +D + P+ GLW+ Y+V K ++ K SKM+++I++R + QL+ ++Q Q+VS + FKG F SKP +I F++ ++ + Y++K+ L N +Y+ N C+L V ENL DF+ I F PPGP+S GM+CE + F+P +N+DL G + F +Q+ F++P+KC TKKC +S+D+++I F T V+GE+ + + L N G GT C K + E + E N + +D+Q+ L D + T ++ + + I P+ ++ I ++P+ G+ F++ F++ + R +G AIDVPV++ TV + ICM +RLYQD+I ++R+ AL+L+F V E++ ++E+LP TG+IQA + Q+K L R+S+ + YFD + +L+ + +A+Q+K V F+ QAI+TT+DL+I + ++FG CTI E I L N +LPQEFGF G P++++IQPN G+GTILP ETL L V+F P KAK Y F+L C+S I+R F VSC +GVHP L+LS + F T++ D S +++ N+HLS N H P+IG P+GP +F+F++P NSPI I P G++ P ++ + V F+P L I EA+ ++N+++E + +K W+ ++++ +D P +V P + EL +P I S +F + +L + F F + C +A+G I DR + + ++ +N+L+LEL CP++ P +++ S+ GK +FG+ R K I++QNI E + ++LNPNG F N KL LV+SF + + Q + ++ L++T + A + + N G VL E + N S L + + + L +SH + T+N N +F+I P G++ GK+Q V+FSPDH S F +++K +F++ +++G + M+++ P ++ P E++ D +++E L PI++ Y D A VGC+RT S ++ E+S+D ++ L GF+++PS+G +E K+INISW+PP++++ L S L+ L+G+ T
Sbjct: 92 IQKTKGELHICRQRMDLLNKQQESLAAEIATEKEANNMAAIGRLQAASRRLQTELENEKDLQSKITAMLKDSENAMWHIEIQKGQF----EDVRKHHEAEAEARQRGLEVHSARQLQREREAMEKSEKNRLLRARKSLHTQKELGLRHQKLVEDAQRNHRIAVKFLKASLGRVREREQKEEMESRTHMQRRMDAVLSLKNNITASRETLKKFQAWGQTRADLAKQKALTEKEVILSQGGDAFKYLFHQRRHQELEAQKRAFEEEQKLRKQEIVNRILKEEAEEEQR--------KRRQHP-------------LSKPINRRTLRD-------KTWQYISDFCEGKSVLTSQQERERELLLYPKTVCVIKAISSESVQVDLGSISTEDEVLAEPDISGLWNKE-SYQVPK--EDMERKPVGGSKMEKDILARTMEQLRSGVVQKQVVSGREFKGRPFNSKPEVIHFKDFDIGKVYKKKITLINATYTINYCKLVGVEENLKDFIHIDFEPPGPMSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGSFSVPLKCSTKKCSLSLDKELIDFGTYVVGETTSRIITLTNVGGLGTKFKFLPDSEFYEMDESQPAMKISSLFTCEEKIIYEKIMTSLSEQQLEVNDSSLVDLQSLKESEKQLDDPEVTTAAVSAMTMIPSEEQAEITLGEVTEGEIGPFSSVKVPITFTPVIPGEVQTKFKVMFKNPQSPPLYFRATGTAIDVPVWVPKATVDLKICMYDRLYQDSITVHTRSKAALRLKFEVCKELRGHIELLPETGYIQAQSTYSVQLKFLPRQSLPEDARKYFDPASRVLEAPMTIRVADQIKPVRFTVQAIVTTSDLEI-----NPSEINFGYCTIYEAIRTEICLSNLSLLPQEFGFVGLPKYVDIQPNDGFGTILPLETLHLDVIFQPIKAKEYKFELVCKSEINRCFKVSCQAVGVHPPLELSHYQIKFSATSLYDTSVSTLYVINSHLSMNKMIHSLPRIGSEEAAPVGPTSFEFLLPPNSPITISPSVGTVLPGKRCLIQVAFQPVLPKEIIYKEASQILNKEIETKPVSQKEIVQRKELWKQSFSVVRVHNRDR---PTRVST------------------------------PQATELQRP-VINSSSTEFQIAQATLSKAFQGKFNRFVIPCVVASGDIKDRKTAEPLSFSPHNTLYLELWCPAVAPFIVVTSHKGKTDFNFGDIAVGHRSVKKITLQNICNEDLTLEYSVLNPNGPFVRLNPFNKLRSGETQTLVLSFSPHENILAQETLDII--TKRGTLSLTLFGMGV-ASMITCSIDGNILNMGYVLARESVSTNFKLQNESSLPIKFWVRLESLSRKKAEAHQQLPKFITSHEQRAEIVGTQNYNGQSVFSIVPVEGLMFPGKAQEFTVTFSPDHESLFFSDLLKVVLFEKKVSHQILLKGAAREHMMFVEGGDPLDV----------PVESLAVVTAFDTEHKEEAEELK-PILVTLNYVQLDTDTTTSPATRELQVGCIRTTQPSPRRTVEFSLDGVSWLQHKGFSIEPSRGSVERGQTKTINISWVPPADFDPDHPLMVSTLLQLRGDVKET 1610
BLAST of Cilia-and flagella-associated protein 74 vs. TrEMBL
Match: A0A571BED3 (Cilia- and flagella-associated protein 74 OS=Mus musculus OX=10090 GN=Cfap74 PE=4 SV=1) HSP 1 Score: 694.501 bits (1791), Expect = 0.000e+0 Identity = 457/1588 (28.78%), Postives = 792/1588 (49.87%), Query Frame = 2
Query: 266 LQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTN-RAVEKAHKKHS---EFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEE-DNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPK--TGKS--SSDEELENDFDFRNNVDNSIET----KIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGT--------------------------NCSIKNMSEIDNETRKVVSREPNSAVNIDVQT------DLLDTKPITTSLDHLKLW-----------------IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEF-------LANKK--WESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSK--IVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIEL------------------SSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKT----DDNNEDEKPSLNTPIILKSAY---SDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYE 4735
+Q+ K EL +C + LNK+ ++ EI T + N+AA+ RLQ+ +R+Q E+E EKDL+ I + +E H ++++G+ E ++ + + E ++ + + A++ E ++ A+ L TQ+ L + + VE A + H +FL+ ++ + R + + E+ ++ +V L + I +E L + ++ L +K L ++ ++ +GG+ + QR+ E+ QK K +K +I ++I+ EE + ++K + P LSK ++ L+ TW+ GKS +S +E E + I+ +++ S +D + P+ GLW+ Y+V K ++ K SKM+++I++R + QL+ ++Q Q+VS + FKG F SKP +I F++ ++ + Y++K+ L N +Y+ N C+L V ENL DF+ I F PPGP+S GM+CE + F+P +N+DL G + F +Q+ F++P+KC TKKC +S+D+++I F T V+GE+ + + L N G GT C K + E + E N + +D+Q+ L D + T ++ + + I P+ ++ I ++P+ G+ F++ F++ + R +G AIDVPV++ TV + ICM +RLYQD+I ++R+ AL+L+F V E++ ++E+LP TG+IQA + Q+K L R+S+ + YFD + +L+ + +A+Q+K V F+ QAI+TT+DL+I + ++FG CTI E I L N +LPQEFGF G P++++IQPN G+GTILP ETL L V+F P KAK Y F+L C+S I+R F VSC +GVHP L+LS + F T++ D S +++ N+HLS N H P+IG P+GP +F+F++P NSPI I P G++ P ++ + V F+P L I EA+ ++N+++E + +K W+ ++++ +D P +V P + EL +P I S +F + +L + F F + C +A+G I DR + + ++ +N+L+LEL CP++ P +++ S+ GK +FG+ R K I++QNI E + ++LNPNG F N KL LV+SF + + Q + ++ L++T + A + + N G VL E + N S L + + + L +SH + T+N N +F+I P G++ GK+Q V+FSPDH S F +++K +F++ +++G + M+++ P ++ P E++ D +++E L PI++ Y D A VGC+RT S ++ E+S+D ++ L GF+++PS+G +E K+INISW+PP++++
Sbjct: 92 IQKTKGELHICRQRMDLLNKQQESLAAEIATEKEANNMAAIGRLQAASRRLQTELENEKDLQSKITAMLKDSENAMWHIEIQKGQF----EDVRKHHEAEAEARQRGLEVHSARQLQREREAMEKSEKNRLLRARKSLHTQKELGLRHQKLVEDAQRNHRIAVKFLKASLGRVREREQKEEMESRTHMQRRMDAVLSLKNNITASRETLKKFQAWGQTRADLAKQKALTEKEVILSQGGDAFKYLFHQRRHQELEAQKRAFEEEQKLRKQEIVNRILKEEAEEEQRKRRQHP----------------------LSKPINRRTLRD-------KTWQYISDFCEGKSVLTSQQERERELLLYPKTVCVIKAISSESVQVDLGSISTEDEVLAEPDISGLWNKE-SYQVPK--EDMERKPVGGSKMEKDILARTMEQLRSGVVQKQVVSGREFKGRPFNSKPEVIHFKDFDIGKVYKKKITLINATYTINYCKLVGVEENLKDFIHIDFEPPGPMSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGSFSVPLKCSTKKCSLSLDKELIDFGTYVVGETTSRIITLTNVGGLGTKFKFLPDSEFYEMDESQPAMKISSLFTCEEKIIYEKIMTSLSEQQLEVNDSSLVDLQSLKESEKQLDDPEVTTAAVSAMTMIPSEEQAEITLGEVTEGEIGPFSSVKVPITFTPVIPGEVQTKFKVMFKNPQSPPLYFRATGTAIDVPVWVPKATVDLKICMYDRLYQDSITVHTRSKAALRLKFEVCKELRGHIELLPETGYIQAQSTYSVQLKFLPRQSLPEDARKYFDPASRVLEAPMTIRVADQIKPVRFTVQAIVTTSDLEI-----NPSEINFGYCTIYEAIRTEICLSNLSLLPQEFGFVGLPKYVDIQPNDGFGTILPLETLHLDVIFQPIKAKEYKFELVCKSEINRCFKVSCQAVGVHPPLELSHYQIKFSATSLYDTSVSTLYVINSHLSMNKMIHSLPRIGSEEAAPVGPTSFEFLLPPNSPITISPSVGTVLPGKRCLIQVAFQPVLPKEIIYKEASQILNKEIETKPVSQKEIVQRKELWKQSFSVVRVHNRDR---PTRVST------------------------------PQATELQRP-VINSSSTEFQIAQATLSKAFQGKFNRFVIPCVVASGDIKDRKTAEPLSFSPHNTLYLELWCPAVAPFIVVTSHKGKTDFNFGDIAVGHRSVKKITLQNICNEDLTLEYSVLNPNGPFVRLNPFNKLRSGETQTLVLSFSPHENILAQETLDII--TKRGTLSLTLFGMGV-ASMITCSIDGNILNMGYVLARESVSTNFKLQNESSLPIKFWVRLESLSRKKAEAHQQLPKFITSHEQRAEIVGTQNYNGQSVFSIVPVEGLMFPGKAQEFTVTFSPDHESLFFSDLLKVVLFEKKVSHQILLKGAAREHMMFVEGGDPLDV----------PVESLAVVTAFDTEHKEEAEELK-PILVTLNYVQLDTDTTTSPATRELQVGCIRTTQPSPRRTVEFSLDGVSWLQHKGFSIEPSRGSVERGQTKTINISWVPPADFD 1590
BLAST of Cilia-and flagella-associated protein 74 vs. TrEMBL
Match: A0A3M6T4P1 (Uncharacterized protein OS=Pocillopora damicornis OX=46731 GN=pdam_00005199 PE=4 SV=1) HSP 1 Score: 666.381 bits (1718), Expect = 0.000e+0 Identity = 451/1635 (27.58%), Postives = 817/1635 (49.97%), Query Frame = 2
Query: 266 LQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKAHKKHSEFLRKTVEKER---AVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQE-------RIKPSKVSP-VLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQ--------NVKLKNSGAKGTNCSIKNMSEIDNETRKV----VSREPN----------------SAVNID--------------------VQTDLLDTKPITTS--------LDHLKLW------IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCP-YFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGK--SLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSS-----HFYGQT-----------------NLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWP-FETMKTDDNNEDEKPSLNTP-----------------IILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVTQSLEIRGFL 4822
+ QF++E K C HI EL K+ + EIE + N+A+ +RLQS ++ EI EKD+ IL ++ +AE + +E+GK + L E E K E+Q+ + +N ++ +R + E + + A ++ ++ S+FL++T+ + R A + ++++ E M+ ++ L I KENL A+ ++ + + ++EL+ R+ + +G NP+ E+ +RK++ A ++ Q +E++++I + I+ EE KK+ + PQL+ E RI + P T S S Y + T + P + S E+ E+DF ++ + + + + + PEF+GLW+ + K + SKM+QE+M R L + + I+Q Q+ + + FKG +F SKP +I+F++ +V + Y++K++LTN SY+ N C+ +S L DF+ + F PPG +S G+TC+F + F+P LNEDL+G I F +Q+ F++P++C+TKKC++S D ++F + V+GE+ + NV ++S + M+E E + + EP SAV + + DL P T S L+ L++ I P+ ++ + ++P +G+ + F I F D + +R G IDVPV++E + V + +CM +RLYQD I+ N+RA +AL+L+F V E++++LE+LP T +IQA F AQ+K L R++ L ++CP YFD E+ +L+ + +A+Q + V F+ +AI+T++D++ V +DFG CT+ E+ +T+ L NK +LPQ+FGF G P+ +++QPN G+GT+LP E+ ++ ++F+ KAK Y F L C++ I R F + C +GV P L+LS ++ TA+ D I + N+H S N +TH P+IG GP+V +GP +F+F++P ++P+ + P G+++ E+ V V F P + + ++ EA ++ R+ E+E L+ E+ + + K K ++ +SA K G S L A + + SN++ L+LL + F V C +A G ++ +S + +++N+++LE+HCP+++P L+++S+SG+ ++DF + K +IQNIS E ++ S++L+P+G F + NA+R L+P A ++VSF P F + + L + K + + + +++G + G L+ + I+N S+L++ Y ++L S H Q L N N + +F+ P +GVI G+S+ + +SF+PDH S + + V ++I++ E S+ G+ WP ++ D SL + +KS + DG + + +GC+++ ++ KK+ ++ DN LGF+ +P K ++ +K+I W PP+ ++ ++TS ++T+K + + + ++G +
Sbjct: 66 VSQFRDEHKKCQEHIDELQKDKELVSNEIEEAETQNNMASFYRLQSYHDKLCAEIGAEKDVEREILNRLEEAEFALAQATVEQGKFL-----LAGQELEKEEKLVEKQKAEWSAVRLRKENMSAAVAERRRRLREKERERKHRQAIA--AAKRNREQASKFLKETMSRVRQKEAEEEELSRRNMEDRMQ---AILCLKGNIEANKENLRALQARDAKLRQEKEEEELQERQEIFAEGLNPDEELT-RRKRIRQAYERRQ-----RERQVEIVENILKEEKQMKKRYQQQPQLWPEKQRERVKRISRRRPKPKFFKSTSGSSSVEYSADVEDT--------GGQHTPGKLGAVSSEDDEDDFSPYGET-----SRDEAKEGDSEAEGEELAQPEFRGLWEEGVHKASEKKEHRFKFATHIPSKMEQEMMERALMKQRAGIVQKQVAAGREFKGCAFYSKPEVIVFKDFDVGKSYKKKILLTNISYTQNYCKYVGMSHILKDFIEVFFDPPGVMSAGLTCDFAVTFKPMLNEDLVGHIDFLAQTGPFSVPIRCVTKKCQISEDVTEVEFGSQVLGETRKRIINTVTDVNVTQQDSNPTELQVPPEGMAEDAKEPQGAGVTEIPSEPGQQEGESPEKALEPRSGSAVKFEKGLATTEGEVHAPAEETGDQLDVDLTSAAPQTISSIPTETSILEGLRVGKVSSGEIAPFSLVQVEVVFNPTKSGNALAEFEISFSDPLSPPIVIRARGTGIDVPVWVEREVVDLKMCMYDRLYQDAILVNNRATSALRLKFEVCKELRNHLELLPKTAYIQAESQFSAQLKFLPRQT-LPDDCPTYFDGESRLLEAPMVVRVADQTRPVPFTVRAIVTSSDIEFNV-----QAIDFGFCTVLESVRRTVTLTNKSVLPQQFGFCGVPDTVDVQPNDGFGTLLPLESFDVDIMFNAKKAKEYEFDLTCKTGIGREFKLHCRAVGVLPPLELSHSVIKMKATAVSDTCAAHIEVINSHTSANEFTHPVPRIGSGPIVEVGPTSFEFMVPADAPLTVSPAVGTVNAGERCLVHVTFSPRMDPNMVRQEAVRMMEREA--------EAERKRLELASPPEKPQEQNTGQKKKGGKAQAGKSAKASPKRGNTPSNTNLAATTKTPTKTFTVADVIDGSNEYESAHLALLRTYQNAFSRYLVPCFVAPGRCTEPNS-LSCSVHNTIYLEVHCPAVRPHLVVVSDSGRSVVDFANVSLGRSEIKSFTIQNISDEPIKLSSSLLDPHGPFQMLNALRTLDPQATHTVIVSFGPNAPKEFHEVLEV--RCPRNTLYLRLKGRGVQPVISLS-VEDGLLDLGDALVGDTISSSFKISNTSELSITYSLKLDSLSPLRHSRAQVLPRFLPPFSEMSETQQHALGPSNYNGMAVFDCVPAQGVIGPGESKEITISFNPDHPSQLYADEVS------------------------VEINNGEQPYSVRLLGRAWPNIVYLRGWDELMPRAESLRAEVEEEEDEEGKAIQKTVLLTMKSVSTPDGY-EAVERAVEIGCIKS-SIQTKKSSDFYFDNPKEANELGFSFEPQKAGVDIGVKKNIVFRWQPPAGHDPNEPVNTSTMLTVKSDVTTQYKILLQGLI 1627
BLAST of Cilia-and flagella-associated protein 74 vs. TrEMBL
Match: A0A2Y9FZK7 (cilia- and flagella-associated protein 74 OS=Trichechus manatus latirostris OX=127582 GN=LOC101360605 PE=4 SV=1) HSP 1 Score: 664.84 bits (1714), Expect = 0.000e+0 Identity = 465/1605 (28.97%), Postives = 798/1605 (49.72%), Query Frame = 2
Query: 266 LQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKAHKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTA-INSKRKSDKILENKKELELRKSVIEK------GGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEE-DNFKKKIKMMPQLFQERIKPSKVSPVL------GITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTAN-NLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGT---------NCS---------------------------IKNMSEIDN----ETRKVVSREPNSAVNIDVQTDLLDT---KPITTSLDHLKLW------IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDR--SSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSS-------------HFY---GQTNLT-TKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLK----IDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQ--------EAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVT 4795
+Q+ + EL+ C I + ++ EI + NIAA+ RLQ+ R+ E+E E+DL I + + E H ++ EG++ + L+ +E R+ + + +RL KEK + A ++ R S + E + +K HK +FL+ ++ +I ++ ++EM+ +++ ++ +K N+TA + RK + + EL +K+ +EK GG+ ++ Q ++ E+ QK K+++L+I ++ EE + K+K +++P R+ + G+ T+ D E +S K + ++ S E +++D N ++ + PE GLW DYK + ++ K +KMD++I++ + +L+ ++ Q+VS + FKG F SKP +I F++ ++ + Y++K+ L NT+Y+ N C+L V E+L DF+ I F PPGP+S GM+CE + F+P +N+DL G + F +Q+ F++P+KC TKKC +S+D+ +I F + V+GE+ + + L N G GT +C +N+ E + ET K S+EP + + + + T P ++L I P+ ++ I ++PI G+ F++ F++ RV G AIDVPV++ V + ICM +RLYQD+++ ++R+ AL+L+F V E++ ++E+LP TG+IQA ++ Q+K L R S+ + YFD E+ +L+ + +A+Q++ V+F+ AI+TT+DL+I + +DFG CTI E ++ L N +LPQEFGF G P+F++IQPN G+GTILP E+L L V+F P KAK Y+F+L C+S I+R F +SC +GVHP L+LS + F TA+ D S +++ N+HLS N TH P+IG V P+GP +F+F++P +SPI I P G++ P ++ V V F+P L ++ EA +++++ E K KD + + +S S + NR + + EL K E ++ S +F + +L F F+ V C +A+G I DR + + ++ N+L+LEL CP++ P +++ S+ GK + +FG+ R K ISIQNIS E V ++LNPNG F + N KL L +SF ++ + Q + K+ A + ++ N G V+ E + N+S L + + + L S F +TN+ T+N N +F++ P GVI G++Q V+FSPDH S F + ++ +FD+ +++G + M+++ +D P L++ P ++ + E P + LKS +Q A VGC+RT +S KK+ E+S+D+++ L GFTV+PS+G +E K+I+ISW+P ++++ L S L+ L+G+ T
Sbjct: 138 IQKTRGELRACRQRIELVTRQQAVVAAEIAQQKEANNIAAVGRLQATRSRLCTELEKERDLESNITSTLKENENAMWHIEILEGQMDAARKALREEEAAEGRRLQAQAAERLCKEKAAFRKAERNRLLRIRKSMHVQKEHGLRHQKLVEDAQKNHKIAVKFLKASL-------GRIREQEQKEEMESRKHLKRRMDAVLALKNNITANRETLRKFQAWGQARAELAEQKAQVEKEAILAQGGDAFKQLFHQHRRQELEAQKRAFEEEQKQRRLEIVSRLWKEEAEENKRKKQLLPAQTANRLTLRDKTWCYVADFCEGLATTAALYPLDTE----------YSVHPKASQLVEAISSESMQSDPGALTN-----------------PEEETLAVPEISGLWKE--DYKPYQVPKEDVDRKPVGGTKMDKDILAHTMERLRSGVVHKQVVSGREFKGCPFNSKPELIHFKDFDIGKVYKKKITLINTTYTINYCQLVGVEEHLRDFIRIDFNPPGPMSAGMSCEVLVTFKPMINKDLEGNVSFLAQTGGFSVPLKCSTKKCSLSLDKNLIDFGSYVVGETASRVITLTNIGGLGTWYKFQPTSESCEQATSLNTSSVFIYEDKNLYDKVIMPSVSENLFEANESPPMETGKTESKEPEEGCHAESEGVTMTTIMEMPQGEDEAEIQLGEVTEGEIGPFSSIKVPIIFTPIIPGEVQTKFKVVFKNPQYPTLYFRVIGIAIDVPVWVPKPNVDLKICMYDRLYQDSVLVHTRSKAALRLRFEVCKELRAHMELLPETGYIQALSSYSVQLKFLPRHSLSEDAGKYFDKESRVLEAPMTIWVADQIRPVEFTVHAIVTTSDLEI-----SPSEVDFGYCTIYEAIKTSVTLHNHALLPQEFGFVGLPKFVDIQPNDGFGTILPLESLHLDVIFQPTKAKEYSFELVCKSEINREFKLSCRAVGVHPPLELSHYQIKFAATALYDTSVTTLYVINSHLSMNSLTHSVPRIGSQDVAPVGPTSFEFLLPLDSPITISPSVGTVLPGKRCLVQVAFQPVLPEELVRQEALQVLSKEAE-------------AKPSCKDTVTQKRDLRRQSIPSMRLQNRE-----------RQFQVSTSPNPELQKQE-LKASSEEFQAAQAALARAFKGKFDKFVVPCVVASGDIKDRKETETMSFSPQNTLYLELWCPTVAPSIVVTSDKGKTVFNFGDVAVGHRSIKKISIQNISPEDLAVEFSVLNPNGPFVLLNPFNKLPMGETQVLGLSFSPRESILAQETLDII---TKRGTLTLTLLGTGVASVIVCSIEGDILNMGYVIARESVSSTFKLQNHSSLPIKFSMRLDSLSCTRREDRQQLPRFLVSDQRTNVVGTQNHNGQSVFSVVPVEGVIDPGRAQDFTVTFSPDHESLYFSDRLQVVLFDKRISHQILLKGAAREHMMFVEGGDPLDVPVESLTV------IPAFDLEHREGEPHEGPPSSEAEKLKSILVTLEYLQLDTNTPAPPATRELQVGCIRTSQLSPKKSVEFSLDSVSALQHKGFTVEPSRGFVERGQTKTISISWMPTADFDPDRPLMVSALLLLRGDVKET 1667
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Cavefish
Match: cacna1db (cilia and flagella associated protein 74 [Source:NCBI gene;Acc:103033679]) HSP 1 Score: 446.817 bits (1148), Expect = 3.033e-131 Identity = 249/749 (33.24%), Postives = 416/749 (55.54%), Query Frame = 2
Query: 2153 IEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGK-------SLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDR-SSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIE----------------LSSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQV 4327
+ P+ ++ + ++P G+ + +I F + + + G A VPV++ ++ + ICM +RLYQD+I SRA TALK+ F V E+K++++ILP TGFIQA +F+AQ+K L R+S+ + FD +TG+L+V + +A+QV+ V F+ A++TT+DL+ D E +DFG C++ E+ +++L N+ +LPQ+FGF G P+FI++QPN G+GT+LP ++LE+ ++FS KA+ YNFQL C+S I+R F +SC IGV P L+LS ++ F TA+ D+S +++ N++ S N +TH P+IG P+ P+GP+ F F P+NS I + P G + P ++ V V F P LS I+ EAA L+ R E + ES+ A K E + K R ++ + SGK S L + P I+ S+++ GK SLL F F + C ++ G I + S + +Y+ +N+L+LELHCP+I+P L+++SN+G+ I+F + + +++ K +++QNIS E +++S+++ NG F + NA+R++ P L+++F + + K L T + I L + G N G VL E + T+ N+S + V +R+ LSSH + L T+N + +F++ P G + GK+Q + V+F PDH S +R+ ++ + ++V
Sbjct: 786 VSPFSSVKLPVVFTPTIPGETKLDIQITFSQPECEAIMVSACGIAESVPVWVTKPSMDLRICMYDRLYQDSIEVQSRANTALKVTFEVCKEMKNHMDILPKTGFIQAKSSFRAQLKFLPRRSLPVDAKSLFDQDTGVLEVPLVIQVADQVRPVSFTVHAVVTTSDLEF---DRTE--VDFGHCSVFESVRTSVRLTNRSLLPQDFGFVGIPKFIDVQPNDGFGTLLPLQSLEIDLIFSASKAEEYNFQLTCKSGINRDFLLSCCAIGVRPLLELSDSLVEFCGTAVGDRSTAVLYVVNSYTSPNKFTHPAPRIGKNPIPPVGPRLFTFATPENSEITVSPASGRVLPGKRCLVQVSFSPLLSDEDIRAEAAQLLCRSEE---TRVLESQKANAPTANKTETQQETK-----------KGRKSSSNHGSGKQAVKERGSKTSLTPKNDSPLQPPNPAEIQNGSDEYAAGKASLLRSFKERFSRYVIPCFVSNGDIMQQESEEPIYSPHNTLYLELHCPAIRPLLVVISNNGETTINFNQVILGQKVLKRVTVQNISSETVKLKSSLMGLNGPFSVQNAMREVRPGDTHTLLLAFTPASAKKYCEKLEV--SCSKMALEFTLCGEGIKP-LVTCSPEGGVMNFGDVLEKETTTQTFTLQNSSSVPVQFRVLLDSLSNHSISRSLPAFLSSHSTPHSTLGTQNYSGQSVFSVAPAEGAVGPGKTQDITVTFQPDHKSRHYRDTLRVQLLNKV 1512 HSP 2 Score: 182.956 bits (463), Expect = 6.717e-46 Identity = 166/617 (26.90%), Postives = 319/617 (51.70%), Query Frame = 2
Query: 278 KNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKA---HKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSK------RKSDKILENKKELELRKSVIEKGGNPNLE-VKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWE----KRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTNCSIKNMSEIDNETRKVVSREPNSAVNI 2086
+ ELK C H++EL + EIE EN A+FRL+++ K + ++++ E++L + + + E + ++E G+ + ++++ DEK + +++E+ +R+++EK + K A + K K + E +EL R ++A KK + FL++T+ N++ Q+ +E+E K+ ++K +K N+ A K ++ ++ E K+ +K ++ G ++ + LQ+ Q ++ +++E K ++++I K++ E D K+K + + L+Q P V T++S K ++ L++I P + E +RP + + SS ++ + DF + + +K ++ + PEF GLW T ++P+K + M + + + + + K FK +F SKP +++F++ +V + Y++K+ LTN S+STN CRL VS+NL+DF+++ F PPGPLS GM CE + ++P LNE+L GEI+ S + F++P+KC K+C M +D ++I F T V+G+++ + + L N GA GT ++ S N +S+ P+ +
Sbjct: 110 REELKACRLHLAELGEVRDDLETEIEKEIQAENSVAVFRLRARHKCLCVQLQEEEELEAHLGMVLQEHELELCEVEVELGRFLSLRQQVEQDEKNFNAQKQEKHNQRVQREKNIAKGARLRVLRAKNEQEKALRE---QAELDQRRRDQALLSQKKAAVFLKQTL-------NRMRQKESEREGKRKEQMKKRMEAAASLKANIAATQEKLCVRQVKEKARLQEQKEHENHQKETLQAAGVNSIRLMHLQKAQEDLHRKQEAFQERQKARRIEIVSKLLLE-DEMKEKRQAL--LYQP--TPPHV------TQSSALKQREQRLLQSILPPSTPAEREAGLTQRPASSRVSSRSDVSSSVDF---IKDERSSKGSEAAEREGEEREYLFEPEFTGLWSRMHQDFTRTTDEHIPVKESNPWSMKTTLERSLASKAAK-----KTAGGKEFK-KAFVSKPEMVLFKDFDVGKTYKKKIKLTNVSHSTNYCRLLGVSQNLVDFISVSFEPPGPLSAGMACELEAVYKPLLNENLDGEIQLQSATGPFSVPIKCTVKRCEMVVDSRLIDFGTHVVGQTISRVITLTNRGALGTRFALAPHS--TNRLPPQLSKHPSPTTQV 694
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006006.1 (pep scaffold:Pmarinus_7.0:GL478690:18571:32696:-1 gene:ENSPMAG00000005425.1 transcript:ENSPMAT00000006006.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 314.309 bits (804), Expect = 1.015e-90 Identity = 141/358 (39.39%), Postives = 224/358 (62.57%), Query Frame = 2
Query: 2165 GEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLS 3238
G ++ + ++P GD F + F D + ++R S A+DVPV+++ V + IC +RLYQD+++ ++RA TAL+L+F V ++++YLE+LP G+IQA F AQ+K L R+S+ + YFD +T +L+ + + +Q K V F+ AI+T++DL ++DFG CTI E+ T++L N IL Q+FGF PE++++QPN+G+GT+LP +T+ + ++FS KAK Y F L C+S +R F + C GVHP L+LS +L F TA+ D S + + N+H S N Y+H P++G G V P+GP +FQF++P + P+ + P G++ P +KQ V + F P LS
Sbjct: 501 GYVKLEVVFTPTVPGDTRATFHVYFTDPGSPRLEVRASALAVDVPVWVQRPNVDLKICTFDRLYQDSVLVHNRATTALRLKFEVCRQLQNYLEVLPKNGYIQAQSTFSAQLKFLPRQSMAVDAPGYFDKDTCVLEAPVLIRVGDQTKPVPFTVHAIITSSDLHF-----DREVIDFGCCTIFESVKATVKLTNDSILAQDFGFVTIPEYVDVQPNHGFGTVLPLDTVLIDIIFSATKAKEYKFDLTCKSGTNRDFQLRCVATGVHPPLELSDSLLRFRATAVGDTSVASLHVVNSHTSANQYSHPVPRVGRGDVAPVGPTSFQFVVPPDCPLSVSPAVGTVAPGQKQLVRLLFRPVLS 853 HSP 2 Score: 181.03 bits (458), Expect = 1.111e-46 Identity = 122/395 (30.89%), Postives = 208/395 (52.66%), Query Frame = 2
Query: 833 SVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLE-IAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNS--NRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRK--VFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGT 2002
S+ L I KENL A+ +++ +++ ++E R +++ GGNP EV L+RK+LE + K + +K +++ I + I+ EE ++K + Q R P+ + + ++L D ++ +E E +TG + D D + + + + + T S +++ ++I E+ GLW+ + KT + +K + + + VL + +P+ R +G SF SKPN+I F+N +V + Y+ KVVLTN SY++N CR + +SE+L D V+++F PPG +S GM+ E + FE NEDL GE++F +QS F +PV+C TKKC +++DR I F T V+GE++ + L N GA GT
Sbjct: 3 SIMTLKQSIANNKENLRALRARQVAEQAARMQEEERERAAILASGGNPT-EVLLKRKRLEEFERTKREFEEQVKARQVDIIEHILKEE----AEMKRRSKQQQRRQHPAAATASRDAEGAAGTRLADPRVIRLLE--------EALGETGNRCRSPSPDLDGDGEHVGLSGLGETVAMATRSPGYEEEEDDLIQSEYPGLWEPEDE---QKTKRDSEVKTRPFGPSEADGGGPVLEE------KPKGQRRLGLTVQGRSFSSKPNVIAFKNFDVGKIYKLKVVLTNVSYTSNFCRYKGMSEHLEDSVSVRFKPPGQMSAGMSFELMVTFEATKNEDLNGELQFAAQSGPFTVPVQCTTKKCDVTVDRSKIDFGTHVVGETIRRTFTLVNRGALGT 375
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006010.1 (pep scaffold:Pmarinus_7.0:GL478690:25063:35984:-1 gene:ENSPMAG00000005425.1 transcript:ENSPMAT00000006010.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 186.037 bits (471), Expect = 6.450e-49 Identity = 160/582 (27.49%), Postives = 292/582 (50.17%), Query Frame = 2
Query: 266 LQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKAHKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLE-IAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRK--VFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGT 2002
+QQ EL+ EL +E Q T +++ + ++ N A+ LQ Q R + E++ E+ L+ + + I++ + +E G+ ++ ++ E+ ++ ER E R+ E +A +S+E +R ++ M+ + + + ++ FLR T+ K R + + ++ + ++ S+ L I KENL A+ +++ +++ ++E R +++ GGNP EV L+RK+LE + K + +K +++ I + I+ EE ++K + Q R P+ + + ++L D ++ +E E +TG S + + + + IK+QT ++ E+ GLW+ + KT + +K + + + VL + +P+ R +G SF SKPN+I F+N +V + Y+ KVVLTN SY++N CR + +SE+L D V+++F PPG +S GM+ E + FE NEDL GE++F +QS F +PV+C TKKC +++DR I F T V+GE++ + L N GA GT
Sbjct: 29 IQQTGEELRGSRLREQELRREQQITQQQMRQAADSTNTVAMTLLQRQETRTKEEVDTEQALQDTLSQTISQHQLALCALDVELGRFSQVQQHVQEAERAIATQQHERAEGRIALEMAKATSAQRSVEAWERQRSQEMMALDERRRQAAEEIARNQQRARHFLRDTMAKVRQREAEEEEKVRQDNDRRLKSIMTLKQSIANNKENLRALRARQVAEQAARMQEEERERAAILASGGNPT-EVLLKRKRLEEFERTKREFEEQVKARQVDIIEHILKEE----AEMKRRSKQQQRRQHPAAATASRDAEGAAGTRLADPRVIRLLE--------EALGETGNSQAPVCVPMTHSGHSPGNAQRVLSIKVQT-------ICMLESEYPGLWEPEDE---QKTKRDSEVKTRPFGPSEADGGGPVLEE------KPKGQRRLGLTVQGRSFSSKPNVIAFKNFDVGKIYKLKVVLTNVSYTSNFCRYKGMSEHLEDSVSVRFKPPGQMSAGMSFELMVTFEATKNEDLNGELQFAAQSGPFTVPVQCTTKKCDVTVDRSKIDFGTHVVGETIRRTFTLVNRGALGT 581
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005986.1 (pep scaffold:Pmarinus_7.0:GL478690:7340:16256:-1 gene:ENSPMAG00000005408.1 transcript:ENSPMAT00000005986.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 141.354 bits (355), Expect = 3.740e-36 Identity = 104/393 (26.46%), Postives = 190/393 (48.35%), Query Frame = 2
Query: 3689 PDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQA--ITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSSHFYG---------------QTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLK-GETSVTQSLEIR 4813
P LI +S+SG+ +DFG+ + + K I+++NIS + +V ++ +P G F + NA+R + P LV++F + F + L + +AQ +T +E L + G VL + N+S + V + + L S G +++ T+N + L +F++ P +G+I G L V+FSPDH S R+ + ++ +F ++E+ ++G R +YL+ P ++ +++ +E + D KP L T ++S +DDG + F VGCVR + S +K GE+ D GFTV+P +G +EA +KS+ ++W P + + S S+ ++L+ G+ + S+ +R
Sbjct: 1 PPLICVSDSGRTTLDFGQVCIGQTVTKRITVENISHQHLKV--SLKDPLGPFTLLNALRPISPGCTHTLVLAFAALQQRQFHERLEVQAGGAVLTLTLVGRAQLPLVTCSVEEPCL-----SLGPVLSCSTASAGFKLHNDSSVPVSFLLTLDSESPGLHSSLPILPPSGGSSSSSVGTQNHSGLSVFSVQPVQGIIPVGGDTELLVTFSPDHASARYSDRLRLLLFGKIEFRVIELKGCAVKRMLYLEGGDPLDV-AVESLAPLPVWEDDGSSDVVAAVKPFLLT---MRSTQTDDGFSPVTRELF-VGCVRPVQPSSRKGGEFIFDIAEARAK-GFTVEPVRGTVEAGHKKSVRVTWSPVDGQDPWAPASASVSLSLRSGDATEAVSVLLR 380
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005992.1 (pep scaffold:Pmarinus_7.0:GL478690:7673:13929:-1 gene:ENSPMAG00000005408.1 transcript:ENSPMAT00000005992.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 94.3597 bits (233), Expect = 3.899e-21 Identity = 63/219 (28.77%), Postives = 110/219 (50.23%), Query Frame = 2
Query: 4172 TKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENM----LSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLK-GETSVTQSLEIR 4813
T+N + L +F++ P +G+I G L V+FSPDH S R+ + ++ +F + E+ ++G R +YL+ P ++ L+ + D KP L T ++S +DDG + F VGCVR + S +K GE+ D GFTV+P +G +EA +KS+ ++W P + + S S+ ++L+ G+ + S+ +R
Sbjct: 76 TQNHSGLSVFSVQPVQGIIPVGGDTELLVTFSPDHASARYSDRLRLLLFGKKEFRVIELKGCAVKRMLYLEGGDPLDVAVESLAPLPVCTHYHHVGAARVDVVAAVKPFLLT---MRSTQTDDGFSPVTRELF-VGCVRPVQPSSRKGGEFIFDIAEARA-KGFTVEPVRGTVEAGHKKSVRVTWSPVDGQDPWAPASASVSLSLRSGDATEAVSVLLR 289
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001803.1 (pep scaffold:Pmarinus_7.0:GL476330:20174:144478:-1 gene:ENSPMAG00000001615.1 transcript:ENSPMAT00000001803.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 51.6026 bits (122), Expect = 1.847e-6 Identity = 58/276 (21.01%), Postives = 111/276 (40.22%), Query Frame = 2
Query: 1595 FQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPG----PLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTNCSIKNMSEIDNETRKVVSREPNSAVNIDVQTDLLDTKPITTSLDHLKLWIEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQF 2410
FQ P+ I F+N E E Y+ + L N RL VS+ D K + P ++PGM F I F P+ +D ++ ++ +KF +PV+ + + + V+ F + + + ++N G + N S+ +T + S EP + I S+ ++ + ++P GD I ++ ++ DL G A+DV V ++ +V M+ + Q +++ ++R Q+
Sbjct: 98 FQPFPSEITFQNFEPCETYEVPLNLRNNDRVP---RLVKVSQE--DSPYFKIISPNDVGTKVAPGMASTFRIVFTPEQKKDYTHQVVCVTEREKFMVPVRAIGSRAILDFP-DVVAFSQRPVKLRSERTLLVRNVGNRHANFSL--------QTERPFSVEPVYGALV-----------IGESM-------------QVTVAFTPQTTGDHSAELWIHYDTGEDICVDLH--GAAVDVNVRLDKNSVVMERTFISMASQRSVLLSNRMDAIAHFQW 333
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Nematostella
Match: EDO49993 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7REY5]) HSP 1 Score: 433.335 bits (1113), Expect = 4.019e-135 Identity = 227/649 (34.98%), Postives = 369/649 (56.86%), Query Frame = 2
Query: 1508 EIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTNCSIKNMSEIDNETRKV---------------------VSREPNSAVNIDVQTDLLDT--------------------KPITT-------------SLDHL-KLWIEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLE 3289
EIM + L + + I+Q Q+ + + FKG +F SKP +I+F++ +V + Y++KV+LTN SY+ N C+ +S L DF+ + F PPG +S G+TC+F + F P LNE+L G+I F +Q+ F++P+KC+TKKC++S+D + F + V+GE+ + V L NSGA GT ++ N++ V V P + V D DT P+ T SLD + I P+ ++ + ++P+ +G + +F I F D + LR G IDVPV++E + V + ICM +RLYQD I+ ++RA +AL+L+F V E++++LE+LP +IQAG F AQ+K L R S+L + YFD ETG+L+ + +A+Q + V F+ ++++T++D++ V + LDFG CT+ E+ T+ L NK +LPQ +GF G P+ +++QPN G+GT+LP E+L++ ++F+ KAK Y F+L C+S I R F + C +GV P L+ S ++ TA+ D + + N+H S N +TH P+IG GP+V +GP +F+F++PD +P+ + P + + V F P L ++ EA ++ R+ E
Sbjct: 1 EIMEQSLMKQRAGIVQKQVAAGREFKGCAFYSKPEVIVFKDFDVGKSYRKKVLLTNISYTQNFCKYLGMSHVLKDFIEVAFDPPGVMSAGLTCDFSVTFNPMLNENLEGQIDFLAQTGPFSVPIKCMTKKCQLSVDAVEVDFGSQVVGETRKKVVVLTNSGALGTEFGFLKIT--GNQSSSVYHYYITCFECLLEGLRGDTPAVEAAPGTMVTFDEPPGSNDTPDQQEEAEAQPDESGPVAMGSPVPTEVTVMEGLRVGKVSLDSVVSGSIAPFSTVQLEVVFAPVKSGSTMADFEISFSDPLSPPIMLRGRGNGIDVPVWVERELVDLKICMYDRLYQDAILVHNRATSALRLKFEVCKELRNHLELLPKMAYIQAGSQFSAQLKFLPRLSLLEDCKEYFDPETGVLEAPMVIRVADQTRPVPFTVRSVVTSSDIEFSVKE-----LDFGFCTVYESVRHTVTLTNKSVLPQPYGFCGVPDTVDVQPNDGFGTLLPLESLDVDIIFNAKKAKEYTFELTCKSGIGREFKLPCRAVGVLPPLEFSHSVVEMKATAVGDTCSTHLEVMNSHTSANEFTHPVPRIGTGPIVDVGPTSFEFVVPDGAPLTVSPA--------RCIIHVTFTPVLDPDEVRKEAVKILEREAE 634
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Nematostella
Match: EDO49992 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7REY3]) HSP 1 Score: 97.0561 bits (240), Expect = 2.526e-21 Identity = 79/364 (21.70%), Postives = 155/364 (42.58%), Query Frame = 2
Query: 3848 ICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSS-----HFYGQ--------------TN---LTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPF--------------ETMKTDDN---NEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVTQSLEIRGFL 4822
+ NA+R LEP A +V+SF P + + K L I K + +T + ++ G + G L+N+ ++N S+L++ Y ++L S H Q TN + N N L + + P +G I AG+++ + V+F+PDH S+ + + M ++I++ E ++ G+ WP E++ D + +ED+K T ++ +YS A+ +G +R+ + KK+ ++ DN GFT DP K ++ ++ + W+PP ++ ++TS ++T+K + + + ++G +
Sbjct: 1 MLNALRVLEPQATHTVVMSFGPCAPSEYHEVLEI--RCPKNTLYIRLKGKGVTPVISLS-VEGGVLDLGDALVNDTVTTTFKMSNTSELSISYNLKLDSLSPLRHSRAQVLPRFLQPYNDLAETNQHVVGPSNFNGLAVIDCVPAQGTIGAGETKEITVTFTPDHPSELYAD------------------------EMAVEINNGERAHTVKLIGRAWPHIVYVRGWDELTPCVESLVPDGDEQEDEDQKAEQKTVLLTFKSYSTPEGFLAAERILEIGSIRS-STQTKKSSDFFFDNPKEANEKGFTFDPMKAGVDIGVKRDVVCKWLPPPGHDPNEPVNTSTMLTVKADVTTQYKILLKGLI 336
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Nematostella
Match: EDO28663 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T5U7]) HSP 1 Score: 83.5741 bits (205), Expect = 4.972e-17 Identity = 66/325 (20.31%), Postives = 136/325 (41.85%), Query Frame = 2
Query: 3965 KKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSS-----HFYGQT---------NLTTKNK--------NSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPF--------------ETMKTDDN---NEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVTQSLEIRGFL 4822
K L I K + +T + ++ G + G L+N+ ++N S+L++ Y ++L S H Q +L N+ N L + + P +G I AG+++ + V+F+PDH S+ + + M + I++ E ++ G+ WP E++ D + +ED+K T ++ +YS A+ +G +R+ KK+ ++ DN GF+ DP K ++ ++ + W+PP ++ ++TS ++T+K + + + ++G +
Sbjct: 11 KNTLYIRLKGKGVTPVISLS-VEGGVLDLGDALVNDTVTTTFKMSNTSELSISYNLKLDSLSPLRHSRAQVLPRFLQPYNDLAETNQHVVGPSNFNGLAVIDCVPAQGTIGAGETKEITVTFTPDHPSEHYAD------------------------EMAVDINNGERAHTVKLIGRAWPHIVYVRGWDELTPCVESLVPDGDEQEDEDQKAEQKTVLLTFKSYSTPEGFLAAERILEIGSIRS-NTQTKKSSDFFFDNPKEANEKGFSFDPMKAGVDIGVKRDVVCKWLPPPGHDPNEPVNTSTMLTVKADVTTQYKILLKGLI 309
BLAST of Cilia-and flagella-associated protein 74 vs. Planmine SMEST
Match: SMESG000043377.1 (SMESG000043377.1) HSP 1 Score: 3110.86 bits (8064), Expect = 0.000e+0 Identity = 1592/1595 (99.81%), Postives = 1594/1595 (99.94%), Query Frame = 2
Query: 41 MTDNYESTMNNEEEYIYDNSIKXXXXXXXXXXXXXXXXTQDKETKDFDMKLRQSMINMRSILXXXXXXXXXXYEKLQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKAHKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTNCSIKNMSEIDNETRKVVSREPNSAVNIDVQTDLLDTKPITTSLDHLKLWIEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVTQSLEIRGFLL 4825
MTDNYESTMNNEEEYIYDNSIKDDLGLSLSESDESMELTQDKETKDFDMKLRQSMINMRSILNNIKVNVKNKYEKLQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDEKLYERKEKERQEKRLRKEKKLTKNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKAHKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKF+TSVIGESLVQNVKLKNSGAKGTNCSIKNMSEIDNETRKVVSREPNSAVNIDVQTDLLDTKPITTSLDHLKLWIEPYGEQEILIKWSPIFAG+CVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVH ALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAKSKKSDKKSNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVTQSLEIRGFLL
Sbjct: 1 MTDNYESTMNNEEEYIYDNSIKDDLGLSLSESDESMELTQDKETKDFDMKLRQSMINMRSILNNIKVNVKNKYEKLQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDEKLYERKEKERQEKRLRKEKKLTKNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKAHKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFDTSVIGESLVQNVKLKNSGAKGTNCSIKNMSEIDNETRKVVSREPNSAVNIDVQTDLLDTKPITTSLDHLKLWIEPYGEQEILIKWSPIFAGECVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHAALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAKSKKSDKKSNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVTQSLEIRGFLL 1595
BLAST of Cilia-and flagella-associated protein 74 vs. Planmine SMEST
Match: SMESG000043377.1 (SMESG000043377.1) HSP 1 Score: 3095.06 bits (8023), Expect = 0.000e+0 Identity = 1584/1587 (99.81%), Postives = 1586/1587 (99.94%), Query Frame = 2
Query: 65 MNNEEEYIYDNSIKXXXXXXXXXXXXXXXXTQDKETKDFDMKLRQSMINMRSILXXXXXXXXXXYEKLQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDXXXXXXXXXXXXXXXXXXXXXXXXNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKAHKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFETSVIGESLVQNVKLKNSGAKGTNCSIKNMSEIDNETRKVVSREPNSAVNIDVQTDLLDTKPITTSLDHLKLWIEPYGEQEILIKWSPIFAGDCVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHPALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAXXXXXXXXXNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVTQSLEIRGFLL 4825
MNNEEEYIYDNSIKDDLGLSLSESDESMELTQDKETKDFDMKLRQSMINMRSILNNIKVNVKNKYEKLQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDEKLYERKEKERQEKRLRKEKKLTKNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKAHKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKF+TSVIGESLVQNVKLKNSGAKGTNCSIKNMSEIDNETRKVVSREPNSAVNIDVQTDLLDTKPITTSLDHLKLWIEPYGEQEILIKWSPIFAG+CVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVH ALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAKSKKSDKKSNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVTQSLEIRGFLL
Sbjct: 1 MNNEEEYIYDNSIKDDLGLSLSESDESMELTQDKETKDFDMKLRQSMINMRSILNNIKVNVKNKYEKLQQFKNELKLCDNHISELNKEGQTTLEEIETSRNNENIAALFRLQSQLKRIQIEIEGEKDLRILILEKINKAEQDYIHSQLEEGKLVFEEEKLKTDEKLYERKEKERQEKRLRKEKKLTKNALQSLEFDKRNSAKIMLETQQNSELTNRAVEKAHKKHSEFLRKTVEKERAVKNQIAQETAEKEMKKHISVQKLNSEIVQIKENLTAINSKRKSDKILENKKELELRKSVIEKGGNPNLEVKLQRKQLEIAKQKEQLMNAIKEKKLKIADKIVAEEDNFKKKIKMMPQLFQERIKPSKVSPVLGITETSLSKLYDKEQLKTIEKPEIWSTWEKRPKTGKSSSDEELENDFDFRNNVDNSIETKIKLQTNSNRKDDTNVITPEFQGLWDTTIDYKVSKTANNLPIKAKSASKMDQEIMSRVLHQLKENIIQPQIVSRKVFKGHSFQSKPNIIMFENLEVNEKYQQKVVLTNTSYSTNQCRLESVSENLLDFVTIKFMPPGPLSPGMTCEFDIYFEPKLNEDLLGEIKFTSQSDKFAIPVKCLTKKCRMSIDRQVIKFDTSVIGESLVQNVKLKNSGAKGTNCSIKNMSEIDNETRKVVSREPNSAVNIDVQTDLLDTKPITTSLDHLKLWIEPYGEQEILIKWSPIFAGECVENFRIQFEDEDNKDFDLRVSGRAIDVPVYIEDQTVSMDICMLERLYQDTIVFNSRAATALKLQFHVPSEIKDYLEILPSTGFIQAGQAFKAQIKLLARKSILTENCPYFDIETGILDVLIKTSIANQVKTVDFSFQAILTTTDLDIIVPDSKENLLDFGPCTIKETKIQTIQLVNKCILPQEFGFTGCPEFIEIQPNYGYGTILPHETLELKVLFSPPKAKNYNFQLKCQSLIDRAFTVSCTGIGVHAALDLSTCILSFPETAIDDQSKLDIFIQNNHLSTNIYTHEPPQIGDGPVVPIGPQTFQFIIPDNSPIFIHPKFGSLDPNEKQKVSVFFEPNLSIHSIKTEAATLVNRKLEFLANKKWESEIAILKDQIKDERVEPEKVDAKSKKSDKKSNRSATDKNKSGKSLEELMAGGPDSYELVKPESIEIDSNDFLKGKLSLLEKFDFNFEIVNVKCAIAAGHISDRSSKIVYNLYNSLHLELHCPSIKPDLIILSNSGKPIIDFGETVCEKRLKKIISIQNISKECFQVRSTILNPNGVFHICNAVRKLEPNAVANLVVSFISKDPGIFQSNWSLFGENRKKILNITFKAQAITADLEFKELQNGEFNCGSVLINEFNERPLTITNNSKLNVDYRIELSSHFYGQTNLTTKNKNSLCIFNITPERGVIAAGKSQTLAVSFSPDHVSDRFREIVKFFIFDQVEYSSFVIRGEGKIRTMYLKIDSPENMLSMDYFGQTWPFETMKTDDNNEDEKPSLNTPIILKSAYSDDGLIQEAKFCFTVGCVRTLAVSQKKNGEYSIDNLNVLTPLGFTVDPSKGQIEADTEKSINISWIPPSNYEIGSILSTSMLVTLKGETSVTQSLEIRGFLL 1587 The following BLAST results are available for this feature:
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 2
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of Cilia-and flagella-associated protein 74 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 3
BLAST of Cilia-and flagella-associated protein 74 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 1
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 3
BLAST of Cilia-and flagella-associated protein 74 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Cilia-and flagella-associated protein 74 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30002606 ID=SMED30002606|Name=Cilia-and flagella-associated protein 74|organism=Schmidtea mediterranea sexual|type=transcript|length=4841bpback to top protein sequence of SMED30002606-orf-1 >SMED30002606-orf-1 ID=SMED30002606-orf-1|Name=SMED30002606-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1596bp MTDNYESTMNNEEEYIYDNSIKDDLGLSLSESDESMELTQDKETKDFDMKback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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