NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3

Overview
NameNADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
Smed IDSMED30002592
Length (bp)637
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (SMED30002592) t-SNE clustered cells

Violin plots show distribution of expression levels for NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (SMED30002592) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (SMED30002592) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (SMED30002592) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002592

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30002592SMESG000016331.1 dd_Smed_v4_1078_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30002592SMESG000016331.1 dd_Smed_v4_1078_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30002592SMESG000016331.1 dd_Smed_v4_1078_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Celegans
Match: C18E9.4 (pep chromosome:WBcel235:II:8964238:8964901:-1 gene:WBGene00007684.1 transcript:C18E9.4.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C18E9.4)

HSP 1 Score: 55.0694 bits (131), Expect = 5.543e-10
Identity = 32/93 (34.41%), Postives = 49/93 (52.69%), Query Frame = 3 
Query:   24 MGGHGKSDWHCPDWRSY-KVNDSRDVRQHVERLAKLGLKDPWARNHVWKF---VPHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEALGYSS 290
            MGG     +  P++  Y    D   + QH +RLA++GLKDPW RN+V+ +    PH+V   A   +++  G   G+     LI +EEA  Y +
Sbjct:    1 MGGGHHEPFKIPNYSIYSNFRDFPQLAQHEKRLAQIGLKDPWIRNYVYLYDRKYPHVVGQWAHFKKLILPGWKAGVAFTAALIFVEEAYQYKT 93
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Celegans
Match: C18E9.4 (pep chromosome:WBcel235:II:8964242:8964890:-1 gene:WBGene00007684.1 transcript:C18E9.4.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:C18E9.4)

HSP 1 Score: 55.0694 bits (131), Expect = 5.543e-10
Identity = 32/93 (34.41%), Postives = 49/93 (52.69%), Query Frame = 3 
Query:   24 MGGHGKSDWHCPDWRSY-KVNDSRDVRQHVERLAKLGLKDPWARNHVWKF---VPHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEALGYSS 290
            MGG     +  P++  Y    D   + QH +RLA++GLKDPW RN+V+ +    PH+V   A   +++  G   G+     LI +EEA  Y +
Sbjct:    1 MGGGHHEPFKIPNYSIYSNFRDFPQLAQHEKRLAQIGLKDPWIRNYVYLYDRKYPHVVGQWAHFKKLILPGWKAGVAFTAALIFVEEAYQYKT 93
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Fly
Match: ND-B12 (gene:FBgn0034645 transcript:FBtr0300679)

HSP 1 Score: 53.1434 bits (126), Expect = 3.419e-9
Identity = 31/86 (36.05%), Postives = 40/86 (46.51%), Query Frame = 3 
Query:   24 MGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRV-LFSGLPQGIIAGFILIGLEEAL 278
            MGGH    +  P   +YKV     + +  E L + GLKDPW RN VW++ P         L   LF GL  G  A    + +E AL
Sbjct:    1 MGGHHGEPYTVPHASTYKVESVPQLVEVKEALGRQGLKDPWLRNEVWRYEPKAFGTHRSRLNTFLFRGLGVGFCAFLATVAVEYAL 86
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Fly
Match: ND-B12 (gene:FBgn0034645 transcript:FBtr0300680)

HSP 1 Score: 53.1434 bits (126), Expect = 3.419e-9
Identity = 31/86 (36.05%), Postives = 40/86 (46.51%), Query Frame = 3 
Query:   24 MGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRV-LFSGLPQGIIAGFILIGLEEAL 278
            MGGH    +  P   +YKV     + +  E L + GLKDPW RN VW++ P         L   LF GL  G  A    + +E AL
Sbjct:    1 MGGHHGEPYTVPHASTYKVESVPQLVEVKEALGRQGLKDPWLRNEVWRYEPKAFGTHRSRLNTFLFRGLGVGFCAFLATVAVEYAL 86
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Fly
Match: ND-B12 (gene:FBgn0034645 transcript:FBtr0113102)

HSP 1 Score: 53.1434 bits (126), Expect = 3.419e-9
Identity = 31/86 (36.05%), Postives = 40/86 (46.51%), Query Frame = 3 
Query:   24 MGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRV-LFSGLPQGIIAGFILIGLEEAL 278
            MGGH    +  P   +YKV     + +  E L + GLKDPW RN VW++ P         L   LF GL  G  A    + +E AL
Sbjct:    1 MGGHHGEPYTVPHASTYKVESVPQLVEVKEALGRQGLKDPWLRNEVWRYEPKAFGTHRSRLNTFLFRGLGVGFCAFLATVAVEYAL 86
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Fly
Match: ND-B12 (gene:FBgn0034645 transcript:FBtr0113101)

HSP 1 Score: 53.1434 bits (126), Expect = 3.419e-9
Identity = 31/86 (36.05%), Postives = 40/86 (46.51%), Query Frame = 3 
Query:   24 MGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRV-LFSGLPQGIIAGFILIGLEEAL 278
            MGGH    +  P   +YKV     + +  E L + GLKDPW RN VW++ P         L   LF GL  G  A    + +E AL
Sbjct:    1 MGGHHGEPYTVPHASTYKVESVPQLVEVKEALGRQGLKDPWLRNEVWRYEPKAFGTHRSRLNTFLFRGLGVGFCAFLATVAVEYAL 86
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Fly
Match: ND-B12 (gene:FBgn0034645 transcript:FBtr0071663)

HSP 1 Score: 53.1434 bits (126), Expect = 3.419e-9
Identity = 31/86 (36.05%), Postives = 40/86 (46.51%), Query Frame = 3 
Query:   24 MGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRV-LFSGLPQGIIAGFILIGLEEAL 278
            MGGH    +  P   +YKV     + +  E L + GLKDPW RN VW++ P         L   LF GL  G  A    + +E AL
Sbjct:    1 MGGHHGEPYTVPHASTYKVESVPQLVEVKEALGRQGLKDPWLRNEVWRYEPKAFGTHRSRLNTFLFRGLGVGFCAFLATVAVEYAL 86
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Xenopus
Match: mms19 (MMS19 homolog, cytosolic iron-sulfur assembly component [Source:Xenbase;Acc:XB-GENE-979258])

HSP 1 Score: 56.6102 bits (135), Expect = 3.096e-10
Identity = 29/82 (35.37%), Postives = 47/82 (57.32%), Query Frame = 3 
Query:   33 HGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEAL 278
            HG++  H PD+R +K+  +  + +  +RLA+ GL+DPW RN  W+++     P A    V+F G   G  A  + +G+E AL
Sbjct:    8 HGQAKVHLPDYRQWKIEGT-PLEEVQKRLARQGLRDPWLRNEAWRYMGGYAKP-ATFKDVVFRGFKWGFAAFVVALGVEYAL 87
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. UniProt/SwissProt
Match: sp|Q02365|NDUB3_BOVIN (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 OS=Bos taurus OX=9913 GN=NDUFB3 PE=1 SV=2)

HSP 1 Score: 47.3654 bits (111), Expect = 3.597e-6
Identity = 25/82 (30.49%), Postives = 41/82 (50.00%), Query Frame = 3 
Query:   33 HGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEAL 278
            HG S    PD++ +K+ +   +    E+LA  GL+DPW RN  W+++    + ++  +  L  G   G  A  + IG E  L
Sbjct:    9 HGPSKMELPDYKQWKI-EGTPLETVQEKLAARGLRDPWGRNEAWRYMGGFANNVS-FVGALLKGFKWGFAAFVVAIGAEYYL 88
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. UniProt/SwissProt
Match: sp|Q0MQD0|NDUB3_PONPY (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 OS=Pongo pygmaeus OX=9600 GN=NDUFB3 PE=3 SV=3)

HSP 1 Score: 46.595 bits (109), Expect = 7.714e-6
Identity = 23/82 (28.05%), Postives = 39/82 (47.56%), Query Frame = 3 
Query:   33 HGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEAL 278
            HG      PD++ +K+ +   +    ++LA  GL+DPW RN  W+++      ++    V F G   G  A  + +G E  L
Sbjct:    5 HGHHKMELPDYKQWKI-EGTPLETIQKKLAAKGLRDPWGRNEAWRYMGGFTKSVS-FFDVFFKGFKWGFAAFVVAVGAEYYL 84
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. TrEMBL
Match: A0A183MVG3 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS20038 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.244e-15
Identity = 35/73 (47.95%), Postives = 51/73 (69.86%), Query Frame = 3 
Query:   39 KSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLF-SGLPQGIIAGFI 254
            K +W CPDW++Y + DS  +++HVE+LAKLGLKDPW RN+ W+++P I  P +   R +F S LP G+  G +
Sbjct:    2 KINWKCPDWKTYTL-DSPPLKEHVEKLAKLGLKDPWVRNYAWQYLPKIYKPRSQWYREIFLSRLPHGVALGIV 73
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. TrEMBL
Match: A0A183KFK0 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS13795 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.721e-15
Identity = 34/73 (46.58%), Postives = 51/73 (69.86%), Query Frame = 3 
Query:   39 KSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLR-VLFSGLPQGIIAGFI 254
            K +W CPDW++Y + D   +++HVE+LAKLGLKDPWARN+ W+++P I  P +   R +L + LP G+  G +
Sbjct:    2 KINWKCPDWKTYTL-DPPPLKEHVEKLAKLGLKDPWARNYAWQYLPKIYKPRSQRFREILLARLPHGVALGIV 73
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. TrEMBL
Match: A0A430QRG7 (NADH dehydrogenase (Ubiquinone) 1 beta subcomplex subunit 3 OS=Schistosoma bovis OX=6184 GN=DC041_0010654 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 9.646e-15
Identity = 34/73 (46.58%), Postives = 51/73 (69.86%), Query Frame = 3 
Query:   39 KSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLR-VLFSGLPQGIIAGFI 254
            K +W CPDW++Y + D   +++HVE+LAKLGLKDPWARN+ W+++P I  P +   R +L + LP G+  G +
Sbjct:    8 KINWKCPDWKTYTL-DPPPLKEHVEKLAKLGLKDPWARNYAWQYLPKIYKPRSQRFREILLARLPHGVALGIV 79
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. TrEMBL
Match: A0A267FVJ4 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig004930g4 PE=4 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.503e-14
Identity = 42/89 (47.19%), Postives = 54/89 (60.67%), Query Frame = 3 
Query:   21 DMGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLFS-GLPQGIIAGFILIGLEEALGY 284
            D  GHG + W  PDWR YK+ D+  V+QHV+RLA +GLKDPWARN VW++      P    +  L + G P GI A   +I LE+  GY
Sbjct:   16 DSHGHG-TKWIPPDWRQYKIQDAPRVQQHVDRLAAIGLKDPWARNEVWRYHSEFYKPPNRWIMWLITRGAPYGIAAFGTVIVLEKLYGY 103
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. TrEMBL
Match: A0A267F0R0 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009864g2 PE=4 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.636e-14
Identity = 42/89 (47.19%), Postives = 54/89 (60.67%), Query Frame = 3 
Query:   21 DMGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLFS-GLPQGIIAGFILIGLEEALGY 284
            D  GHG + W  PDWR YK+ D+  V+QHV+RLA +GLKDPWARN VW++      P    +  L + G P GI A   +I LE+  GY
Sbjct:   16 DSHGHG-TKWIPPDWRQYKIQDAPRVQQHVDRLAAIGLKDPWARNEVWRYHSEFYKPPNRWIMWLITRGAPYGIAAFGTVIVLEKLYGY 103
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Cavefish
Match: ndufb3 (NADH:ubiquinone oxidoreductase subunit B3 [Source:NCBI gene;Acc:103047320])

HSP 1 Score: 46.9802 bits (110), Expect = 5.647e-7
Identity = 28/89 (31.46%), Postives = 45/89 (50.56%), Query Frame = 3 
Query:   24 MGG-HGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEALGYS 287
            MGG HG S    PD++++K   +  +    +RLA+ GL+DPWARN  W++       +          L +G  AGF +  +  A+ Y+
Sbjct:    1 MGGDHGHSKLSMPDYKTWKWEGT-PLEATQQRLARRGLRDPWARNEAWRYTGSFGKAV-----TFKDVLMRGFKAGFAVFAVALAVEYA 83
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Medaka
Match: ndufb3 (NADH:ubiquinone oxidoreductase subunit B3 [Source:NCBI gene;Acc:101161088])

HSP 1 Score: 50.447 bits (119), Expect = 2.554e-8
Identity = 30/86 (34.88%), Postives = 42/86 (48.84%), Query Frame = 3 
Query:   24 MGG-HGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEAL 278
            MGG HG S    PDWR ++   +  +    +RLA  GLKDPWARN  W++       +     VL  G   G  A  + + +E A+
Sbjct:    1 MGGDHGHSKLSMPDWRQWETKGT-PLEFTQQRLAARGLKDPWARNEAWRYSGGFARAVT-FSDVLLRGFKWGFAAFTVALAIEYAV 84
[Close]
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Planmine SMEST
Match: SMESG000016331.1 (SMESG000016331.1)

HSP 1 Score: 214.542 bits (545), Expect = 3.186e-72
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 3 
Query:   24 MGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEALGYSSGSLLDETIEKLKSKN 335
            MGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEALGYSSGSLLDETIEKLKSKN
Sbjct:    1 MGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFVPHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEALGYSSGSLLDETIEKLKSKN 104
The following BLAST results are available for this feature:
BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
C18E9.45.543e-1034.41pep chromosome:WBcel235:II:8964238:8964901:-1 gene... [more]
C18E9.45.543e-1034.41pep chromosome:WBcel235:II:8964242:8964890:-1 gene... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ND-B123.419e-936.05gene:FBgn0034645 transcript:FBtr0300679[more]
ND-B123.419e-936.05gene:FBgn0034645 transcript:FBtr0300680[more]
ND-B123.419e-936.05gene:FBgn0034645 transcript:FBtr0113102[more]
ND-B123.419e-936.05gene:FBgn0034645 transcript:FBtr0113101[more]
ND-B123.419e-936.05gene:FBgn0034645 transcript:FBtr0071663[more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mms193.096e-1035.37MMS19 homolog, cytosolic iron-sulfur assembly comp... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q02365|NDUB3_BOVIN3.597e-630.49NADH dehydrogenase [ubiquinone] 1 beta subcomplex ... [more]
sp|Q0MQD0|NDUB3_PONPY7.714e-628.05NADH dehydrogenase [ubiquinone] 1 beta subcomplex ... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183MVG37.244e-1547.95Uncharacterized protein OS=Schistosoma margrebowie... [more]
A0A183KFK07.721e-1546.58Uncharacterized protein OS=Schistosoma curassoni O... [more]
A0A430QRG79.646e-1546.58NADH dehydrogenase (Ubiquinone) 1 beta subcomplex ... [more]
A0A267FVJ41.503e-1447.19Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267F0R01.636e-1447.19Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ndufb35.647e-731.46NADH:ubiquinone oxidoreductase subunit B3 [Source:... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ndufb32.554e-834.88NADH:ubiquinone oxidoreductase subunit B3 [Source:... [more]
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BLAST of NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000016331.13.186e-72100.00SMESG000016331.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002592 ID=SMED30002592|Name=NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3|organism=Schmidtea mediterranea sexual|type=transcript|length=637bp
AATCTATATAAATCTTATATGATATGGGTGGTCATGGTAAATCTGACTGG
CATTGTCCAGATTGGCGATCCTATAAAGTTAATGATTCCAGAGATGTAAG
ACAGCACGTAGAACGATTAGCGAAGCTCGGCTTAAAAGATCCTTGGGCAA
GAAATCACGTATGGAAATTTGTTCCTCATATTGTATCTCCAATTGCCCCA
ATGTTAAGAGTTCTTTTTTCTGGACTACCACAAGGAATTATTGCAGGTTT
TATTCTAATAGGATTGGAGGAGGCTTTGGGTTACAGTAGCGGAAGCTTGT
TGGATGAAACGATAGAAAAATTGAAATCGAAAAATTAGATTGTTTTAGCT
GTGTAGAGATAGAATAAATTTCTAGTTGATTTATTGATTGAATGCGATTG
GATAGGAGGAATCATTATTGAGTGATTTGCGGTTAATTGCGCATGAGATG
AATTATTGATTTATTTACGGATGTGTATTGGTTGGTTTGGTTGTTAATTA
GTGTGATATTGATTGGGTATGTGGAGCAACATTATTTATGCCAGTCTGGC
GGGCGTGATTTGCAATTACTTATTATTATATATTATTATCCAGTTGAGTT
TTGTGTCTGAATTAAGAATTTTTAAAGAATTTTTGGC
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protein sequence of SMED30002592-orf-1

>SMED30002592-orf-1 ID=SMED30002592-orf-1|Name=SMED30002592-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=105bp
MGGHGKSDWHCPDWRSYKVNDSRDVRQHVERLAKLGLKDPWARNHVWKFV
PHIVSPIAPMLRVLFSGLPQGIIAGFILIGLEEALGYSSGSLLDETIEKL
KSKN*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000429neoblast
Vocabulary: INTERPRO
TermDefinition
IPR012576NDUFB3
Vocabulary: biological process
TermDefinition
GO:0022900electron transport chain
Vocabulary: cellular component
TermDefinition
GO:0005739mitochondrion
GO:0005747mitochondrial respiratory chain complex I
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012576NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3PFAMPF08122NDUF_B12coord: 39..87
e-value: 4.0E-9
score: 36.4