SMED30002579
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30002579 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 6
Alignments
SMED30002579 aligns in the following genomic locations:
Homology
BLAST of SMED30002579 vs. Ensembl Human
Match: ELF5 (E74 like ETS transcription factor 5 [Source:HGNC Symbol;Acc:HGNC:3320]) HSP 1 Score: 50.447 bits (119), Expect = 7.034e-7 Identity = 32/116 (27.59%), Postives = 59/116 (50.86%), Query Frame = 3 Query: 591 EYLQSNCKSDSRVANVRPRDAPRNQI---GMLTFILDLLNHPYFEPC--IKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 +Y SNC S + + R + + F+ DLL P E C ++WED++ F+++ S+ +A W K+ND ++E +R +RY G +++D ++ Y+F K + Sbjct: 67 DYADSNCLKTSGIKSQDCHSHSRTSLQSSHLWEFVRDLLLSPE-ENCGILEWEDREQGIFRVVKSEALAKMWGQRKKNDRMTYEKLSRALRYYYKTGILERVD--RRLVYKFGKNA 179
BLAST of SMED30002579 vs. Ensembl Human
Match: ELF3 (E74 like ETS transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:3318]) HSP 1 Score: 51.6026 bits (122), Expect = 1.251e-6 Identity = 30/96 (31.25%), Postives = 50/96 (52.08%), Query Frame = 3 Query: 639 RPRDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 + + APR + FI D+L HP E +KWE++ FK + S+ VA W K+N ++E +R +RY +++D ++ Y+F K S Sbjct: 265 KSKHAPRG-THLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRR-LVYKFGKNS 358
BLAST of SMED30002579 vs. Ensembl Human
Match: ELF3 (E74 like ETS transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:3318]) HSP 1 Score: 51.6026 bits (122), Expect = 1.251e-6 Identity = 30/96 (31.25%), Postives = 50/96 (52.08%), Query Frame = 3 Query: 639 RPRDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 + + APR + FI D+L HP E +KWE++ FK + S+ VA W K+N ++E +R +RY +++D ++ Y+F K S Sbjct: 265 KSKHAPRG-THLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRR-LVYKFGKNS 358
BLAST of SMED30002579 vs. Ensembl Human
Match: ELF3 (E74 like ETS transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:3318]) HSP 1 Score: 51.6026 bits (122), Expect = 1.251e-6 Identity = 30/96 (31.25%), Postives = 50/96 (52.08%), Query Frame = 3 Query: 639 RPRDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 + + APR + FI D+L HP E +KWE++ FK + S+ VA W K+N ++E +R +RY +++D ++ Y+F K S Sbjct: 265 KSKHAPRG-THLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRR-LVYKFGKNS 358
BLAST of SMED30002579 vs. Ensembl Human
Match: ELF5 (E74 like ETS transcription factor 5 [Source:HGNC Symbol;Acc:HGNC:3320]) HSP 1 Score: 50.447 bits (119), Expect = 1.494e-6 Identity = 32/116 (27.59%), Postives = 59/116 (50.86%), Query Frame = 3 Query: 591 EYLQSNCKSDSRVANVRPRDAPRNQI---GMLTFILDLLNHPYFEPC--IKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 +Y SNC S + + R + + F+ DLL P E C ++WED++ F+++ S+ +A W K+ND ++E +R +RY G +++D ++ Y+F K + Sbjct: 135 DYADSNCLKTSGIKSQDCHSHSRTSLQSSHLWEFVRDLLLSPE-ENCGILEWEDREQGIFRVVKSEALAKMWGQRKKNDRMTYEKLSRALRYYYKTGILERVD--RRLVYKFGKNA 247
BLAST of SMED30002579 vs. Ensembl Celegans
Match: ets-4 (Transcription factor ets-4 [Source:UniProtKB/Swiss-Prot;Acc:A8WFJ9]) HSP 1 Score: 47.3654 bits (111), Expect = 8.736e-6 Identity = 24/83 (28.92%), Postives = 45/83 (54.22%), Query Frame = 3 Query: 666 IGMLTFILDLLNHP-YFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQF 911 + + FI +LL+ P + C++W D+ TFKI +S L+A YW K +++ +R +R G ++ + K++ Y+F Sbjct: 288 VHLWQFIRELLDQPKQYSACVRWVDRDEGTFKIESSLLLARYWGQRKNRSQMNYDKLSRSLRQYYKKGIIQKPEKKQRLVYKF 370
BLAST of SMED30002579 vs. Ensembl Celegans
Match: ets-4 (Transcription factor ets-4 [Source:UniProtKB/Swiss-Prot;Acc:A8WFJ9]) HSP 1 Score: 47.3654 bits (111), Expect = 8.736e-6 Identity = 24/83 (28.92%), Postives = 45/83 (54.22%), Query Frame = 3 Query: 666 IGMLTFILDLLNHP-YFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQF 911 + + FI +LL+ P + C++W D+ TFKI +S L+A YW K +++ +R +R G ++ + K++ Y+F Sbjct: 288 VHLWQFIRELLDQPKQYSACVRWVDRDEGTFKIESSLLLARYWGQRKNRSQMNYDKLSRSLRQYYKKGIIQKPEKKQRLVYKF 370
BLAST of SMED30002579 vs. Ensembl Celegans
Match: ets-4 (Transcription factor ets-4 [Source:UniProtKB/Swiss-Prot;Acc:A8WFJ9]) HSP 1 Score: 47.3654 bits (111), Expect = 8.736e-6 Identity = 24/83 (28.92%), Postives = 45/83 (54.22%), Query Frame = 3 Query: 666 IGMLTFILDLLNHP-YFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQF 911 + + FI +LL+ P + C++W D+ TFKI +S L+A YW K +++ +R +R G ++ + K++ Y+F Sbjct: 288 VHLWQFIRELLDQPKQYSACVRWVDRDEGTFKIESSLLLARYWGQRKNRSQMNYDKLSRSLRQYYKKGIIQKPEKKQRLVYKF 370
BLAST of SMED30002579 vs. Ensembl Zebrafish
Match: elf3 (E74-like factor 3 (ets domain transcription factor, epithelial-specific ) [Source:ZFIN;Acc:ZDB-GENE-030131-8760]) HSP 1 Score: 53.1434 bits (126), Expect = 1.956e-7 Identity = 31/104 (29.81%), Postives = 55/104 (52.88%), Query Frame = 3 Query: 618 DSRVANVRPRDAPRNQIGMLTFILDLLNHP-YFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKSI 926 D + + + + APR + FI D+L HP + +KWED++ FK + S+ VA W K+N + ++E +R +RY +++D ++ Y+F K S Sbjct: 265 DHFIQSKKSKHAPRG-THLWEFIRDILIHPEQNQGLMKWEDRRDGVFKFLKSEAVAQLWGQKKKNSSMTYEKLSRAMRYYYKREILERVDGRR-LVYKFGKNST 366
BLAST of SMED30002579 vs. Ensembl Zebrafish
Match: elf3 (E74-like factor 3 (ets domain transcription factor, epithelial-specific ) [Source:ZFIN;Acc:ZDB-GENE-030131-8760]) HSP 1 Score: 53.1434 bits (126), Expect = 2.364e-7 Identity = 31/104 (29.81%), Postives = 55/104 (52.88%), Query Frame = 3 Query: 618 DSRVANVRPRDAPRNQIGMLTFILDLLNHP-YFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKSI 926 D + + + + APR + FI D+L HP + +KWED++ FK + S+ VA W K+N + ++E +R +RY +++D ++ Y+F K S Sbjct: 307 DHFIQSKKSKHAPRG-THLWEFIRDILIHPEQNQGLMKWEDRRDGVFKFLKSEAVAQLWGQKKKNSSMTYEKLSRAMRYYYKREILERVDGRR-LVYKFGKNST 408
BLAST of SMED30002579 vs. Ensembl Xenopus
Match: ENSXETT00000020719.1 (E74 like ETS transcription factor 3 [Source:NCBI gene;Acc:100144720]) HSP 1 Score: 50.0618 bits (118), Expect = 3.533e-6 Identity = 26/82 (31.71%), Postives = 45/82 (54.88%), Query Frame = 3 Query: 681 FILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 FI D+L HP + +KWED+ FK + S+ VA W K+N + ++E +R +RY +++D ++ Y+F K + Sbjct: 287 FIRDILLHPDLNQGLLKWEDRSEGVFKFLRSEAVAQLWGQKKKNSSMTYEKLSRAMRYYYKREILERVDGRRL-VYKFGKNA 367
BLAST of SMED30002579 vs. Ensembl Mouse
Match: Elf3 (E74-like factor 3 [Source:MGI Symbol;Acc:MGI:1101781]) HSP 1 Score: 51.6026 bits (122), Expect = 8.368e-7 Identity = 30/94 (31.91%), Postives = 49/94 (52.13%), Query Frame = 3 Query: 645 RDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 + APR + FI D+L HP E +KWE++ FK + S+ VA W K+N ++E +R +RY +++D ++ Y+F K S Sbjct: 287 KHAPRG-THLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRRL-VYKFGKNS 378
BLAST of SMED30002579 vs. Ensembl Mouse
Match: Elf3 (E74-like factor 3 [Source:MGI Symbol;Acc:MGI:1101781]) HSP 1 Score: 51.6026 bits (122), Expect = 8.438e-7 Identity = 30/94 (31.91%), Postives = 49/94 (52.13%), Query Frame = 3 Query: 645 RDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 + APR + FI D+L HP E +KWE++ FK + S+ VA W K+N ++E +R +RY +++D ++ Y+F K S Sbjct: 267 KHAPRG-THLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRRL-VYKFGKNS 358
BLAST of SMED30002579 vs. UniProt/SwissProt
Match: sp|Q3UPW2|ELF3_MOUSE (ETS-related transcription factor Elf-3 OS=Mus musculus OX=10090 GN=Elf3 PE=1 SV=1) HSP 1 Score: 51.6026 bits (122), Expect = 5.853e-6 Identity = 30/94 (31.91%), Postives = 49/94 (52.13%), Query Frame = 3 Query: 645 RDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 + APR + FI D+L HP E +KWE++ FK + S+ VA W K+N ++E +R +RY +++D ++ Y+F K S Sbjct: 287 KHAPRG-THLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRRL-VYKFGKNS 378
BLAST of SMED30002579 vs. UniProt/SwissProt
Match: sp|P78545|ELF3_HUMAN (ETS-related transcription factor Elf-3 OS=Homo sapiens OX=9606 GN=ELF3 PE=1 SV=1) HSP 1 Score: 51.6026 bits (122), Expect = 6.009e-6 Identity = 30/96 (31.25%), Postives = 50/96 (52.08%), Query Frame = 3 Query: 639 RPRDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 + + APR + FI D+L HP E +KWE++ FK + S+ VA W K+N ++E +R +RY +++D ++ Y+F K S Sbjct: 265 KSKHAPRG-THLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRR-LVYKFGKNS 358
BLAST of SMED30002579 vs. UniProt/SwissProt
Match: sp|Q4V7E1|ELF3_RAT (ETS-related transcription factor Elf-3 OS=Rattus norvegicus OX=10116 GN=Elf3 PE=2 SV=1) HSP 1 Score: 51.2174 bits (121), Expect = 7.375e-6 Identity = 30/94 (31.91%), Postives = 49/94 (52.13%), Query Frame = 3 Query: 645 RDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 + APR + FI D+L HP E +KWE++ FK + S+ VA W K+N ++E +R +RY +++D ++ Y+F K S Sbjct: 291 KHAPRG-THLWEFIRDILIHPELNEGLMKWENRHEGVFKFLRSEAVAQLWGQKKKNSNMTYEKLSRAMRYYYKREILERVDGRR-LVYKFGKNS 382
BLAST of SMED30002579 vs. UniProt/SwissProt
Match: sp|Q9UKW6|ELF5_HUMAN (ETS-related transcription factor Elf-5 OS=Homo sapiens OX=9606 GN=ELF5 PE=1 SV=2) HSP 1 Score: 50.447 bits (119), Expect = 8.796e-6 Identity = 32/116 (27.59%), Postives = 59/116 (50.86%), Query Frame = 3 Query: 591 EYLQSNCKSDSRVANVRPRDAPRNQI---GMLTFILDLLNHPYFEPC--IKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 +Y SNC S + + R + + F+ DLL P E C ++WED++ F+++ S+ +A W K+ND ++E +R +RY G +++D ++ Y+F K + Sbjct: 145 DYADSNCLKTSGIKSQDCHSHSRTSLQSSHLWEFVRDLLLSPE-ENCGILEWEDREQGIFRVVKSEALAKMWGQRKKNDRMTYEKLSRALRYYYKTGILERVD--RRLVYKFGKNA 257
BLAST of SMED30002579 vs. TrEMBL
Match: A0A1I8GPB9 (ETS domain-containing protein OS=Macrostomum lignano OX=282301 PE=3 SV=1) HSP 1 Score: 68.5514 bits (166), Expect = 3.759e-9 Identity = 32/83 (38.55%), Postives = 55/83 (66.27%), Query Frame = 3 Query: 681 FILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWG-YFKQIDMKKQRTYQFNKKSI 926 F+L LLN+P I+W D+++ FK+++ VA W +K N T ++E FARGIR+ R G YF+ + +K++ Y+F++K++ Sbjct: 240 FLLKLLNNPSCSSLIRWLDRENRLFKVLHPVTVARLWQFIKGNRTDNYECFARGIRHSRDIGKYFEAHEFRKKKVYKFSEKAL 322
BLAST of SMED30002579 vs. TrEMBL
Match: A0A267F0N9 (ETS domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006668g2 PE=3 SV=1) HSP 1 Score: 68.9366 bits (167), Expect = 3.967e-9 Identity = 32/83 (38.55%), Postives = 55/83 (66.27%), Query Frame = 3 Query: 681 FILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWG-YFKQIDMKKQRTYQFNKKSI 926 F+L LLN+P I+W D+++ FK+++ VA W +K N T ++E FARGIR+ R G YF+ + +K++ Y+F++K++ Sbjct: 240 FLLKLLNNPSCSSLIRWLDRENRLFKVLHPVTVARLWQFIKGNRTDNYECFARGIRHSRDIGKYFEAHEFRKKKVYKFSEKAL 322
BLAST of SMED30002579 vs. TrEMBL
Match: A0A267GQD5 (ETS domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006668g3 PE=3 SV=1) HSP 1 Score: 68.9366 bits (167), Expect = 4.113e-9 Identity = 32/83 (38.55%), Postives = 55/83 (66.27%), Query Frame = 3 Query: 681 FILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWG-YFKQIDMKKQRTYQFNKKSI 926 F+L LLN+P I+W D+++ FK+++ VA W +K N T ++E FARGIR+ R G YF+ + +K++ Y+F++K++ Sbjct: 240 FLLKLLNNPSCSSLIRWLDRENRLFKVLHPVTVARLWQFIKGNRTDNYECFARGIRHSRDIGKYFEAHEFRKKKVYKFSEKAL 322
BLAST of SMED30002579 vs. TrEMBL
Match: A0A1I8FYC8 (ETS domain-containing protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006668g1 PE=3 SV=1) HSP 1 Score: 68.9366 bits (167), Expect = 4.162e-9 Identity = 32/83 (38.55%), Postives = 55/83 (66.27%), Query Frame = 3 Query: 681 FILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWG-YFKQIDMKKQRTYQFNKKSI 926 F+L LLN+P I+W D+++ FK+++ VA W +K N T ++E FARGIR+ R G YF+ + +K++ Y+F++K++ Sbjct: 241 FLLKLLNNPSCSSLIRWLDRENRLFKVLHPVTVARLWQFIKGNRTDNYECFARGIRHSRDIGKYFEAHEFRKKKVYKFSEKAL 323
BLAST of SMED30002579 vs. TrEMBL
Match: A0A267FLW3 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig005521g2 PE=3 SV=1) HSP 1 Score: 63.5438 bits (153), Expect = 4.398e-7 Identity = 31/86 (36.05%), Postives = 52/86 (60.47%), Query Frame = 3 Query: 681 FILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWG-YFKQIDMKKQRTYQFNKKSIQTY 935 F+L LL++ I+W DK + FK++ +A W +K N T ++E FARGIR+ R G YF+ ++++ Y+F+ K+I+ Y Sbjct: 606 FLLKLLDNSRCRANIEWVDKYNRLFKVVTPSAIAKLWKHIKGNKTDNYECFARGIRHSRDIGRYFEPHGFRQKKIYKFSPKAIEDY 691
BLAST of SMED30002579 vs. Ensembl Cavefish
Match: elf3 (E74 like ETS transcription factor 3 [Source:NCBI gene;Acc:103047838]) HSP 1 Score: 50.447 bits (119), Expect = 1.291e-6 Identity = 31/104 (29.81%), Postives = 55/104 (52.88%), Query Frame = 3 Query: 615 SDSRVANVRPRDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 S+ V + + +PR + FI D+L HP + +KWED++ FK + S+ VA W K+N + ++E +R +RY +++D ++ Y+F K S Sbjct: 255 SEHFVETKKNKHSPRG-THLWEFIRDILIHPEKNQGLMKWEDRRDGIFKFLKSEAVAQLWGQKKKNSSMTYEKLSRAMRYYYKREILERVDGRR-LVYKFGKNS 356
BLAST of SMED30002579 vs. Ensembl Cavefish
Match: elf3 (E74 like ETS transcription factor 3 [Source:NCBI gene;Acc:103047838]) HSP 1 Score: 50.447 bits (119), Expect = 1.317e-6 Identity = 31/104 (29.81%), Postives = 55/104 (52.88%), Query Frame = 3 Query: 615 SDSRVANVRPRDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 S+ V + + +PR + FI D+L HP + +KWED++ FK + S+ VA W K+N + ++E +R +RY +++D ++ Y+F K S Sbjct: 256 SEHFVETKKNKHSPRG-THLWEFIRDILIHPEKNQGLMKWEDRRDGIFKFLKSEAVAQLWGQKKKNSSMTYEKLSRAMRYYYKREILERVDGRR-LVYKFGKNS 357
BLAST of SMED30002579 vs. Ensembl Sea Lamprey
Match: ehf (ets homologous factor [Source:ZFIN;Acc:ZDB-GENE-060312-7]) HSP 1 Score: 46.9802 bits (110), Expect = 2.575e-7 Identity = 22/61 (36.07%), Postives = 33/61 (54.10%), Query Frame = 3 Query: 672 MLTFILDLLNHPYFEP-CIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRY 851 + FI DLL P P +KWED++ F+ + S VA W K N + ++E +R +RY Sbjct: 5 LWEFIRDLLLQPEQNPGLLKWEDRRQGVFRFVKSDAVARLWGKKKNNSSMTYEKLSRAMRY 65
BLAST of SMED30002579 vs. Ensembl Nematostella
Match: EDO45361 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSZ8]) HSP 1 Score: 48.1358 bits (113), Expect = 1.933e-7 Identity = 30/93 (32.26%), Postives = 47/93 (50.54%), Query Frame = 3 Query: 639 RPRDAPRNQIGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNK 917 RPR +N I + F+L+LL + I W K+ FK+ N + VA W I+K ++E +R IRY G K++ ++ Y+F K Sbjct: 6 RPR--YKNTIHLWEFLLELLADERYSSLIAWSRKEYGEFKLKNQEEVAKRWGILKHRAGMNYEKLSRAIRYYYQQGIIKKVP-GQRLVYKFGK 95
BLAST of SMED30002579 vs. Ensembl Nematostella
Match: EDO44339 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RW27]) HSP 1 Score: 43.1282 bits (100), Expect = 9.464e-6 Identity = 23/82 (28.05%), Postives = 43/82 (52.44%), Query Frame = 3 Query: 666 IGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQF 911 I + F+LDLL + I W +++S FK++N + VA W +K +++ +R +RY G K++ ++ Y+F Sbjct: 3 IHLWEFLLDLLADGRYSSIISWTNRESGEFKLLNQEEVARRWGHLKHRPGMNYDKLSRALRYYYQKGIIKKVHGQRL-VYKF 83
BLAST of SMED30002579 vs. Ensembl Medaka
Match: elf3 (ETS-related transcription factor Elf-3 [Source:NCBI gene;Acc:101162593]) HSP 1 Score: 52.373 bits (124), Expect = 3.748e-7 Identity = 30/94 (31.91%), Postives = 51/94 (54.26%), Query Frame = 3 Query: 645 RDAPRNQIGMLTFILDLLNHPYF-EPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKS 923 + APR + FI D+L HP + +KWED++ FK + S+ VA W K+N + ++E +R +RY +++D ++ Y+F K S Sbjct: 341 KHAPRG-THLWEFIRDILIHPEKNQGLMKWEDRREGVFKFLKSEAVAQMWGQKKKNSSMTYEKLSRAMRYYYKREILERVDGRR-LVYKFGKNS 432
BLAST of SMED30002579 vs. Planmine SMEST
Match: SMESG000069596.1 (SMESG000069596.1) HSP 1 Score: 671.774 bits (1732), Expect = 0.000e+0 Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 3 Query: 27 MDSEDFNHDIIFNDIFCGNCEEVDWEDLSDLIIANSECYHELEINSNYDIFQNSQNSSSSGLEVESNLENDMLLESINSYRDGMWSDSTSSIQKDFINLVKNYELKEFNKTFVEKYFILYNCPLDDILKKSRSQLWMDNCIYGLQLQVYICMVNDYPIPTVQHRNRKVPIQSKSGTSKKKGIVCDRLLEYLQSNCKSDSRVANVRPRDAPRNQIGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKSIQTYKSKRQNNNLTRVLIDNFELRFDS 1004 MDSEDFNHDIIFNDIFCGNCEEVDWEDLSDLIIANSECYHELEINSNYDIFQNSQNSSSSGLEVESNLENDMLLESINSYRDGMWSDSTSSIQKDFINLVKNYELKEFNKTFVEKYFILYNCPLDDILKKSRSQLWMDNCIYGLQLQVYICMVNDYPIPTVQHRNRKVPIQSKSGTSKKKGIVCDRLLEYLQSNCKSDSRVANVRPRDAPRNQIGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKSIQTYKSKRQNNNLTRVLIDNFELRFDS Sbjct: 1 MDSEDFNHDIIFNDIFCGNCEEVDWEDLSDLIIANSECYHELEINSNYDIFQNSQNSSSSGLEVESNLENDMLLESINSYRDGMWSDSTSSIQKDFINLVKNYELKEFNKTFVEKYFILYNCPLDDILKKSRSQLWMDNCIYGLQLQVYICMVNDYPIPTVQHRNRKVPIQSKSGTSKKKGIVCDRLLEYLQSNCKSDSRVANVRPRDAPRNQIGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKSIQTYKSKRQNNNLTRVLIDNFELRFDS 326
BLAST of SMED30002579 vs. Planmine SMEST
Match: SMESG000069596.1 (SMESG000069596.1) HSP 1 Score: 529.25 bits (1362), Expect = 0.000e+0 Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 3 Query: 240 MLLESINSYRDGMWSDSTSSIQKDFINLVKNYELKEFNKTFVEKYFILYNCPLDDILKKSRSQLWMDNCIYGLQLQVYICMVNDYPIPTVQHRNRKVPIQSKSGTSKKKGIVCDRLLEYLQSNCKSDSRVANVRPRDAPRNQIGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKSIQTYKSKRQNNNLTRVLIDNFELRFDS 1004 MLLESINSYRDGMWSDSTSSIQKDFINLVKNYELKEFNKTFVEKYFILYNCPLDDILKKSRSQLWMDNCIYGLQLQVYICMVNDYPIPTVQHRNRKVPIQSKSGTSKKKGIVCDRLLEYLQSNCKSDSRVANVRPRDAPRNQIGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKSIQTYKSKRQNNNLTRVLIDNFELRFDS Sbjct: 1 MLLESINSYRDGMWSDSTSSIQKDFINLVKNYELKEFNKTFVEKYFILYNCPLDDILKKSRSQLWMDNCIYGLQLQVYICMVNDYPIPTVQHRNRKVPIQSKSGTSKKKGIVCDRLLEYLQSNCKSDSRVANVRPRDAPRNQIGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKSIQTYKSKRQNNNLTRVLIDNFELRFDS 255
BLAST of SMED30002579 vs. Planmine SMEST
Match: SMESG000069597.1 (SMESG000069597.1) HSP 1 Score: 300.827 bits (769), Expect = 3.213e-101 Identity = 155/299 (51.84%), Postives = 204/299 (68.23%), Query Frame = 3 Query: 54 IIFNDIFCGNCEEVDWEDLSDLIIANSECYHELEINSNYDIFQNSQNSSSSGLEVESNLENDMLLESINSYRDGMWSDSTSSIQKDFINLVKNYELKEFNKTFVEKYFILYNCPLDDILKKSRSQLWMDNCIYGLQLQVYICMVNDYPIPTVQHRNRKVPI-QSKSGTSKKKGIVCDRLLEYLQSNCKSDSRVANVRPRDAPRNQIGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRYLRHWGYFKQIDMKKQRTYQFNKKSIQTYKSKR 947 +IFNDIF G+ D ++ +I + N++ S L + NLE + L++ + D + + + IQ +F+N +K+ EL + +TF+EKYF Y CP D I++++R+QLWM+NCIYGLQLQ YICMV D P+P V RNRK PI + K+ T +KG+ D +LEYL C++ S + R RDAPRNQIG+L FILDLLNHPY++ CI+W DK SL FKIINSKLVAYYWM+VKENDTC+FENFARGIRYLR G+FKQI+ K QRTYQFNKK+I+ Y+ KR Sbjct: 1 MIFNDIFFGDI------DCTENVIGS-----------------NNEISDEDSLPQDFNLE-ETLIDETDICVDYICPEDSIIIQNNFVNFMKSQELHLYKETFIEKYFTSYKCPTDHIVQRTRTQLWMENCIYGLQLQAYICMVKDIPLPIVPQRNRKQPILKKKNLTQARKGVFQDVVLEYLFQKCRNVSEFVSFRHRDAPRNQIGLLIFILDLLNHPYYKGCIRWVDKGSLIFKIINSKLVAYYWMVVKENDTCNFENFARGIRYLRRVGFFKQINKKAQRTYQFNKKAIKEYEKKR 275
BLAST of SMED30002579 vs. Planmine SMEST
Match: SMESG000035130.1 (SMESG000035130.1) HSP 1 Score: 137.502 bits (345), Expect = 9.169e-38 Identity = 86/235 (36.60%), Postives = 131/235 (55.74%), Query Frame = 3 Query: 294 SSIQKDFINLVKNYELKEFNKTFVEKYF--ILYNCPLDDILKKSRSQLWMDNCIYGLQLQVYICMVNDYPIPTVQHRNRKVPI--QSKSGTSKKKGIV-CDRLLEYLQSNCKSDSRVANVRPRDAPRNQIGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRY-LRHWGYFKQIDMKKQRTYQFNKKSIQTYKSKRQNNNLTRVLID 980 SSI FI + + E + F++TF KYF ++ ++++ +R W + YGL Q +I + +P ++ RNRK+PI + + K KG D + + L NCK + R +PR+Q+ + FILDLL HPY+ CI W +K TF+I NS+ VA+ WM +K NDTC++ENFARGIR+ R+ F + + + YQ K +I YK KR N L + L++ Sbjct: 63 SSILSKFIRYMCSKENEFFSETFCHKYFKRSRFSEYPEELINLNRVDAWNIDWAYGLVYQAFIAQEYNLELPCLKKRNRKIPIIGEKLKRSYKSKGYYDKDPIYDELIKNCKLTEK--EDYKRKSPRDQVLLHIFILDLLMHPYYGFCITWSNKNDFTFEIKNSRAVAHIWMTIKGNDTCNYENFARGIRFKRRNSNMFTRTNKHNRCVYQLTKTAIHDYKVKRSKNELAKQLME 295
BLAST of SMED30002579 vs. Planmine SMEST
Match: SMESG000035130.1 (SMESG000035130.1) HSP 1 Score: 137.502 bits (345), Expect = 1.043e-37 Identity = 86/235 (36.60%), Postives = 131/235 (55.74%), Query Frame = 3 Query: 294 SSIQKDFINLVKNYELKEFNKTFVEKYF--ILYNCPLDDILKKSRSQLWMDNCIYGLQLQVYICMVNDYPIPTVQHRNRKVPI--QSKSGTSKKKGIV-CDRLLEYLQSNCKSDSRVANVRPRDAPRNQIGMLTFILDLLNHPYFEPCIKWEDKKSLTFKIINSKLVAYYWMIVKENDTCSFENFARGIRY-LRHWGYFKQIDMKKQRTYQFNKKSIQTYKSKRQNNNLTRVLID 980 SSI FI + + E + F++TF KYF ++ ++++ +R W + YGL Q +I + +P ++ RNRK+PI + + K KG D + + L NCK + R +PR+Q+ + FILDLL HPY+ CI W +K TF+I NS+ VA+ WM +K NDTC++ENFARGIR+ R+ F + + + YQ K +I YK KR N L + L++ Sbjct: 69 SSILSKFIRYMCSKENEFFSETFCHKYFKRSRFSEYPEELINLNRVDAWNIDWAYGLVYQAFIAQEYNLELPCLKKRNRKIPIIGEKLKRSYKSKGYYDKDPIYDELIKNCKLTEK--EDYKRKSPRDQVLLHIFILDLLMHPYYGFCITWSNKNDFTFEIKNSRAVAHIWMTIKGNDTCNYENFARGIRFKRRNSNMFTRTNKHNRCVYQLTKTAIHDYKVKRSKNELAKQLME 301 The following BLAST results are available for this feature:
BLAST of SMED30002579 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMED30002579 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 3
BLAST of SMED30002579 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30002579 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 2
BLAST of SMED30002579 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of SMED30002579 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 2
BLAST of SMED30002579 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 4
BLAST of SMED30002579 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30002579 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 2
BLAST of SMED30002579 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 1
BLAST of SMED30002579 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30002579 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 2
BLAST of SMED30002579 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 1
BLAST of SMED30002579 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30002579 ID=SMED30002579|Name=SMED30002579|organism=Schmidtea mediterranea sexual|type=transcript|length=1071bpback to top protein sequence of SMED30002579-orf-1 >SMED30002579-orf-1 ID=SMED30002579-orf-1|Name=SMED30002579-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=327bp MDSEDFNHDIIFNDIFCGNCEEVDWEDLSDLIIANSECYHELEINSNYDIback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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