Peptide chain release factor 1

Overview


Name	Peptide chain release factor 1
Smed ID	SMED30002534
Length (bp)	1298

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of Peptide chain release factor 1 (SMED30002534) t-SNE clustered cells

Violin plots show distribution of expression levels for Peptide chain release factor 1 (SMED30002534) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Peptide chain release factor 1 (SMED30002534) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Peptide chain release factor 1 (SMED30002534) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30002534

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
X1 cell	SMED30002534	SMESG000022027.1	SmedASXL_012179	SmedAsxl_ww_GCZZ01	PMID:26114597 Zhu et al., 2015

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Homology

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BLAST of Peptide chain release factor 1 vs. Ensembl Human
Match: MTRF1L (mitochondrial translational release factor 1 like [Source:HGNC Symbol;Acc:HGNC:21051])

HSP 1 Score: 231.106 bits (588), Expect = 2.578e-71
Identity = 119/316 (37.66%), Postives = 207/316 (65.51%), Query Frame = 1

Query:  307 DIFNDIELVNVDEQYLNNS--LLDSDTEPEMADLMRLESNQLSARRDA---LYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGY-FDEIIKSLQEFDEFDQL 1236
            ++   I+L+N  E+ L  +  LL  + E    DL +L  N+++  +     L  ++I +++P  + +E +++ +++ +G GGQE+MLF  ++ + Y+++A  K W FE +   + +E+GG+R    +  G++ Y  +K+E GVHRVQRVP T  QG++HTST+ V +LP   +  + I   D++ ++ ++SG GGQHVNTTDSAVR+VH+PT +V  CQ+E+ Q +NK+ AM +L+  L     EE++N+  + RKI IG++ RS+KIRTYN+ Q+R+T+HRI+K   +++  +   Y  DE+++SL+E+ +++ L
Sbjct:   63 ELLAVIKLLNEKERELRETEHLLHDENE----DLRKLAENEITLCQKEITQLKHQIILLLVPSEETDE-NDLILEVTAGVGGQEAMLFTSEIFDMYQQYAAFKRWHFETLEY-FPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESL 372

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BLAST of Peptide chain release factor 1 vs. Ensembl Human
Match: MTRF1L (mitochondrial translational release factor 1 like [Source:HGNC Symbol;Acc:HGNC:21051])

HSP 1 Score: 186.037 bits (471), Expect = 6.231e-56
Identity = 88/198 (44.44%), Postives = 139/198 (70.20%), Query Frame = 1

Query:  646 GGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGY-FDEIIKSLQEFDEFDQL 1236
            GG+R    +  G++ Y  +K+E GVHRVQRVP T  QG++HTST+ V +LP   +  + I   D++ ++ ++SG GGQHVNTTDSAVR+VH+PT +V  CQ+E+ Q +NK+ AM +L+  L     EE++N+  + RKI IG++ RS+KIRTYN+ Q+R+T+HRI+K   +++  +   Y  DE+++SL+E+ +++ L
Sbjct:   26 GGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESL 223

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BLAST of Peptide chain release factor 1 vs. Ensembl Human
Match: MTRF1 (mitochondrial translation release factor 1 [Source:HGNC Symbol;Acc:HGNC:7469])

HSP 1 Score: 188.734 bits (478), Expect = 2.124e-54
Identity = 115/329 (34.95%), Postives = 196/329 (59.57%), Query Frame = 1

Query:  265 RDLLEQYGRLSSLVDIFNDIELVN--VDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSG--AGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVP---ITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGY-FDEIIKS-LQEFDE 1224
            R L  ++  L+ L  I+ +I+     ++E       L+   E ++ +L   E   +  + + LY++L   ++P    ++ +++ +++ +G   GG     F  ++ + Y+ ++  K+W FE +N    A+ GG+       SG+ VY  LKYE G+HRVQR+P   ++S   +IHT T++VIVLP   +  V++   D++ ++F++ G GGQHVN TDSAVRLVHIPT +VV CQ+E+ Q +NK+ A + L+  L ++  E+   +  S RK+ +GTR++S++IRTYN+TQDR+++HRI     +I+  L  G   D++I+  LQ  DE
Sbjct:  104 RSLNRRHAELAPLAAIYQEIQETEQAIEELESMCKSLNKQDEKQLQELALEERQTIDQKINMLYNELFQSLVPKEKYDK-NDVILEVTAGRTTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTP-ADYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIPEVGLSSRMQRIHTGTMSVIVLPQPDEVDVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKDKRQQQSARKLQVGTRAQSERIRTYNFTQDRVSDHRIAYEVRDIKEFLCGGKGLDQLIQRLLQSADE 430

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Query:  265 RDLLEQYGRLSSLVDIFNDIELVN--VDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSG--AGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVP---ITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGY-FDEIIKS-LQEFDE 1224
            R L  ++  L+ L  I+ +I+     ++E       L+   E ++ +L   E   +  + + LY++L   ++P    ++ +++ +++ +G   GG     F  ++ + Y+ ++  K+W FE +N    A+ GG+       SG+ VY  LKYE G+HRVQR+P   ++S   +IHT T++VIVLP   +  V++   D++ ++F++ G GGQHVN TDSAVRLVHIPT +VV CQ+E+ Q +NK+ A + L+  L ++  E+   +  S RK+ +GTR++S++IRTYN+TQDR+++HRI     +I+  L  G   D++I+  LQ  DE
Sbjct:  104 RSLNRRHAELAPLAAIYQEIQETEQAIEELESMCKSLNKQDEKQLQELALEERQTIDQKINMLYNELFQSLVPKEKYDK-NDVILEVTAGRTTGGDICQQFTREIFDMYQNYSCYKHWQFELLNYTP-ADYGGLHHAAARISGDGVYKHLKYEGGIHRVQRIPEVGLSSRMQRIHTGTMSVIVLPQPDEVDVKLDPKDLRIDTFRAKGAGGQHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQQIIEKDKRQQQSARKLQVGTRAQSERIRTYNFTQDRVSDHRIAYEVRDIKEFLCGGKGLDQLIQRLLQSADE 430

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BLAST of Peptide chain release factor 1 vs. Ensembl Human
Match: MTRF1L (mitochondrial translational release factor 1 like [Source:HGNC Symbol;Acc:HGNC:21051])

HSP 1 Score: 145.976 bits (367), Expect = 4.057e-40
Identity = 78/212 (36.79%), Postives = 135/212 (63.68%), Query Frame = 1

Query:  307 DIFNDIELVNVDEQYLNNS--LLDSDTEPEMADLMRLESNQLSARRDA---LYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPT 927
            ++   I+L+N  E+ L  +  LL  + E    DL +L  N+++  +     L  ++I +++P  + +E +++ +++ +G GGQE+MLF  ++ + Y+++A  K W FE +   + +E+GG+R    +  G++ Y  +K+E GVHRVQRVP T  QG++HTST+ V +LP   +  + I   D++ ++ ++SG GGQHVNTTDSAVR+VH+PT
Sbjct:   63 ELLAVIKLLNEKERELRETEHLLHDENE----DLRKLAENEITLCQKEITQLKHQIILLLVPSEETDE-NDLILEVTAGVGGQEAMLFTSEIFDMYQQYAAFKRWHFETLEY-FPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPT 268

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BLAST of Peptide chain release factor 1 vs. Ensembl Celegans
Match: W03F8.3 (Probable peptide chain release factor 1, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:O44568])

HSP 1 Score: 192.971 bits (489), Expect = 4.996e-57
Identity = 103/296 (34.80%), Postives = 178/296 (60.14%), Query Frame = 1

Query:  370 DSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQG-YFDEIIKSLQEFDEFDQLELALRN 1254
            D     EM  L  ++   +    +    +L   ++P N+L+  S+  +++  GAGGQE+MLF  +LL+ Y+K A   +W ++ + ++    +GG+RS  +  SG +VY  +++E+GVHRVQRVP+  S  ++HTST ++ VLP   +  V +    +K E+ ++SGPGGQ+VN   +AVR+ H  T I V C  E+ Q  N + A ++L  +L++KQ +  + ++ S RK+ +G+++R++KIRTYN+  DR+T+HRI  + + +   L+ G     +I+ LQE    D+L+  + N
Sbjct:   93 DPKETEEMRKLAEVDVESIEENLETELQELAVKIVPLNNLDVLSKCQIELSCGAGGQEAMLFTGELLDMYQKLAAVNSWKWDPLQVDNVP-LGGVRSALIAVSGEKVYAKMRFEAGVHRVQRVPVNDS--RMHTSTASISVLPEPEEVSVVVPSDSVKIEAMRASGPGGQNVNKRSTAVRMTHKETGIAVHCMDERFQHLNIQIAYKRLAAILMQKQVDAMLEKIVSKRKLQVGSKARAEKIRTYNFQHDRVTDHRIQMSITGVAEFLSAGETLQTMIERLQEQHLEDRLDHIIEN 385

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BLAST of Peptide chain release factor 1 vs. Ensembl Celegans
Match: W03F8.3 (Probable peptide chain release factor 1, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:O44568])

HSP 1 Score: 57.7658 bits (138), Expect = 1.514e-10
Identity = 28/83 (33.73%), Postives = 53/83 (63.86%), Query Frame = 1

Query: 1009 IEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQG-YFDEIIKSLQEFDEFDQLELALRN 1254
            ++KQ +  + ++ S RK+ +G+++R++KIRTYN+  DR+T+HRI  + + +   L+ G     +I+ LQE    D+L+  + N
Sbjct:    1 MQKQVDAMLEKIVSKRKLQVGSKARAEKIRTYNFQHDRVTDHRIQMSITGVAEFLSAGETLQTMIERLQEQHLEDRLDHIIEN 83

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BLAST of Peptide chain release factor 1 vs. Ensembl Celegans
Match: T23B5.4 (pep chromosome:WBcel235:IV:12675207:12676000:1 gene:WBGene00011941.1 transcript:T23B5.4a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T23B5.4)

HSP 1 Score: 48.521 bits (114), Expect = 7.912e-7
Identity = 26/80 (32.50%), Postives = 46/80 (57.50%), Query Frame = 1

Query:  820 EIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLL-----IEKQYEEKMNRL 1044
            ++++ D + +     GPGGQ VNT  +AV+L H+PT  V+   + ++  +N   A +++K +L      E  YEE++ RL
Sbjct:   26 DVRKEDCEQKYISGWGPGGQKVNTAQNAVQLTHLPTGTVLKVHESRLLPKNIDIAFERMKFVLDRQINGENCYEEQLKRL 105

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BLAST of Peptide chain release factor 1 vs. Ensembl Celegans
Match: T23B5.4 (pep chromosome:WBcel235:IV:12675216:12675966:1 gene:WBGene00011941.1 transcript:T23B5.4b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T23B5.4)

HSP 1 Score: 47.7506 bits (112), Expect = 2.045e-6
Identity = 27/73 (36.99%), Postives = 43/73 (58.90%), Query Frame = 1

Query:  844 FESFKSS-GPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLL-----IEKQYEEKMNRL 1044
            F+ + S  GPGGQ VNT  +AV+L H+PT  V+   + ++  +N   A +++K +L      E  YEE++ RL
Sbjct:   49 FQKYISGWGPGGQKVNTAQNAVQLTHLPTGTVLKVHESRLLPKNIDIAFERMKFVLDRQINGENCYEEQLKRL 121

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BLAST of Peptide chain release factor 1 vs. Ensembl Fly
Match: mRF1 (gene:FBgn0032486 transcript:FBtr0080444)

HSP 1 Score: 204.527 bits (519), Expect = 2.313e-61
Identity = 127/365 (34.79%), Postives = 209/365 (57.26%), Query Frame = 1

Query:  169 KYLKDELINFQNEKLNEF----------DRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSL-LDSDTEPEMADLMRLES----NQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKN-FSNIQNILNQG-YFDEIIKSLQ 1212
            ++   + ++ QNEKL  +           R+N+   S+S         Y RL+ L  + + +E   V E+++ ++  ++++ + +M +LMR E+    + L  +  AL  +L+++    +D  E   +   + +GAGGQE+MLFA++L   Y  +     W +EE   +   ++GG+R   +  SG   +  L+YE+GVHRVQRVP T   G++HTST ++ V+P   D  V I E D+K E+ ++SG GGQHVNTTDSAVR+VH+PT + V  Q E+ Q +N++ AM++L+  L+++Q E       + +K   G+ +R++KIRTYN+ QDRIT+HRI      N+   L  G     +I+ LQ
Sbjct:   17 RWASTDPLSIQNEKLQAYLESLRQEYYAVRVNAAGNSKS---------YARLAQLEGVVSALEQRRVLERHITSAKDMEAEKDEDMRELMREENEVYVDLLGKQDQALLQELLTL----SDDEEYPALIFGLNAGAGGQEAMLFAQELYEMYTGYFEHMGWEWEEF-ASEGTDIGGLRHASLMVSGEDAFRWLRYEAGVHRVQRVPATEKSGRMHTSTASITVIPRPADIQVHIAEKDLKIETKRASGAGGQHVNTTDSAVRIVHLPTGLAVEAQSERSQLKNRELAMKRLRSRLVQQQLESVEASKMATKKAQQGSLNRNEKIRTYNFVQDRITDHRIQGGTLHNLNGFLKGGDQLSGLIEKLQ 367

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BLAST of Peptide chain release factor 1 vs. Ensembl Fly
Match: CG30100 (gene:FBgn0050100 transcript:FBtr0340022)

HSP 1 Score: 53.5286 bits (127), Expect = 2.318e-8
Identity = 27/65 (41.54%), Postives = 39/65 (60.00%), Query Frame = 1

Query:  823 IKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEK 1017
            ++ESDI+    + SGPGGQ VN T + V L H+PT I V C   ++  +N+  A    + LL+EK
Sbjct:   30 LQESDIEETFMRGSGPGGQAVNKTSNCVFLRHLPTNITVKCHTHRLASKNRVEA----RKLLLEK 90

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BLAST of Peptide chain release factor 1 vs. Ensembl Fly
Match: CG30100 (gene:FBgn0050100 transcript:FBtr0087140)

HSP 1 Score: 53.5286 bits (127), Expect = 2.318e-8
Identity = 27/65 (41.54%), Postives = 39/65 (60.00%), Query Frame = 1

Query:  823 IKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEK 1017
            ++ESDI+    + SGPGGQ VN T + V L H+PT I V C   ++  +N+  A    + LL+EK
Sbjct:   30 LQESDIEETFMRGSGPGGQAVNKTSNCVFLRHLPTNITVKCHTHRLASKNRVEA----RKLLLEK 90

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BLAST of Peptide chain release factor 1 vs. Ensembl Zebrafish
Match: mtrf1l (mitochondrial translational release factor 1-like [Source:ZFIN;Acc:ZDB-GENE-030131-8198])

HSP 1 Score: 245.743 bits (626), Expect = 1.320e-76
Identity = 152/412 (36.89%), Postives = 231/412 (56.07%), Query Frame = 1

Query:   31 CFSIYITF*LIKMNF-------INKFLKTSQLFNNFRFIDYKTFTNISYINMPKYLKDELINFQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSL-LDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQG--YFDEIIKSLQEFDEFDQL 1236
            C ++Y T    K  F       I+K L    +F+   F DY                  L   Q E     + INS  Q+Q+L + D+  Q   ++ L  + ++I+ + V ++ + +   L  D +PE+ +L   E   + A    L  K++S++IP  + ++ SE+ +++ +G GGQE+MLF  ++   Y+ +A    W F+ +  N  +E+GG+R    + SG   Y  LK+E+GVHRVQRVP T S+G+ HTST+ V +LP   +    I   D+K E+ ++SG GGQHVNTTDSAVR+ H+PT  V  CQ+E+ Q +NK TAM+ L+  L   + EE+ +R    RK+ IGTR RS+KIRTYN+ QDRIT+HRI K   ++   L QG    +E+I  LQ+F E + L
Sbjct:   33 CKALYRTINNTKRTFHTSLTANISKILSVDDIFSKQSFQDY------------------LKKKQTEYNTSLNSINS--QNQTLEDADMKTQRTNMTILAHLVHNIKKLEVKQKEMEDMQDLLKDNDPELLELAETEKEAVLADIQDLRQKILSLLIPEEE-SDMSELVLEVSAGVGGQEAMLFTAEIFEMYQNYAAFNGWSFDVLEAN-ASELGGVRHASASVSGPLSYKKLKFEAGVHRVQRVPKTESKGRTHTSTMTVAILPQPTEISFTINPKDLKIETKRASGAGGQHVNTTDSAVRITHLPTGTVAECQQERSQIKNKDTAMKLLRAKLYSARLEEETSRRYQARKLQIGTRGRSEKIRTYNFQQDRITDHRIGKTVHDVHGFL-QGEELLEEMIVFLQQFSEQESL 421

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BLAST of Peptide chain release factor 1 vs. Ensembl Zebrafish
Match: mtrf1 (mitochondrial translational release factor 1 [Source:ZFIN;Acc:ZDB-GENE-050220-8])

HSP 1 Score: 133.265 bits (334), Expect = 5.961e-36
Identity = 75/188 (39.89%), Postives = 118/188 (62.77%), Query Frame = 1

Query:  565 LLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVP---ITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQD 1119
            + + Y+ FA+ KNW FE  N    AE GG+    V  SG  V+  LK+E G HRVQR+P   ++S   +IHT T+ VIVLP + +  ++I   D++ ++F+S G GGQHVNTTDSAVRLVH+PT  V  CQ+ + Q +N++TA++ L+  L +    ++  + ++ R++ + T+S   + RT+   Q+
Sbjct:    1 MFDMYQGFASYKNWDFELFNYTP-AEYGGLHHASVRVSGECVFRWLKFEGGTHRVQRIPEVGLSSRMQRIHTGTMTVIVLPQMEEVDIDIAPKDLRIDTFRSRGAGGQHVNTTDSAVRLVHLPTGTVSECQQFRSQLKNRETALRVLRARLHQCMMGQQREQTHTARRLQVRTKSNIHRQRTHCNCQN 187

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BLAST of Peptide chain release factor 1 vs. Ensembl Zebrafish
Match: mtrf1l (mitochondrial translational release factor 1-like [Source:ZFIN;Acc:ZDB-GENE-030131-8198])

HSP 1 Score: 107.071 bits (266), Expect = 6.431e-26
Identity = 75/257 (29.18%), Postives = 127/257 (49.42%), Query Frame = 1

Query:   31 CFSIYITF*LIKMNF-------INKFLKTSQLFNNFRFIDYKTFTNISYINMPKYLKDELINFQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSL-LDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTST 777
            C ++Y T    K  F       I+K L    +F+   F DY                  L   Q E     + INS  Q+Q+L + D+  Q   ++ L  + ++I+ + V ++ + +   L  D +PE+ +L   E       ++A+ + +  +    +D+   SE+ +++ +G GGQE+MLF  ++   Y+ +A    W F+ +  N  +E+GG+R    + SG   Y  LK+E+GVHRVQRVP T S+G+ HTST
Sbjct:   33 CKALYRTINNTKRTFHTSLTANISKILSVDDIFSKQSFQDY------------------LKKKQTEYNTSLNSINS--QNQTLEDADMKTQRTNMTILAHLVHNIKKLEVKQKEMEDMQDLLKDNDPELLELAETE-------KEAVLADIQDLRQKESDM---SELVLEVSAGVGGQEAMLFTAEIFEMYQNYAAFNGWSFDVLEAN-ASELGGVRHASASVSGPLSYKKLKFEAGVHRVQRVPKTESKGRTHTST 258

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BLAST of Peptide chain release factor 1 vs. Ensembl Zebrafish
Match: si:ch211-275j6.5 (si:ch211-275j6.5 [Source:NCBI gene;Acc:100004874])

HSP 1 Score: 47.3654 bits (111), Expect = 4.030e-6
Identity = 23/62 (37.10%), Postives = 37/62 (59.68%), Query Frame = 1

Query:  823 IKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLL 1008
            + E +++ +  + SGPGGQ  N T + V L HIP+ IVV C + +    N+K A + L++ L
Sbjct:   33 VHEEELEEQFVRGSGPGGQATNKTSNCVVLRHIPSGIVVKCHETRSVDLNRKRAREILREKL 94

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Query:  823 IKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLL 1008
            + E +++ +  + SGPGGQ  N T + V L HIP+ IVV C + +    N+K A + L++ L
Sbjct:   33 VHEEELEEQFVRGSGPGGQATNKTSNCVVLRHIPSGIVVKCHETRSVDLNRKRAREILREKL 94

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BLAST of Peptide chain release factor 1 vs. Ensembl Xenopus
Match: commd1 (copper metabolism domain containing 1 [Source:Xenbase;Acc:XB-GENE-961919])

HSP 1 Score: 241.121 bits (614), Expect = 1.447e-74
Identity = 126/287 (43.90%), Postives = 192/287 (66.90%), Query Frame = 1

Query:  388 EMADLMRLESNQLSARRDA---LYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNIL-NQGYFDEIIKSLQEFDEFDQL 1236
            E  DL  L S ++++ ++    L  K+ S++I   + +E +++ M++ +G GGQE+MLF  ++ + Y+ +A  KNW F+ +   + +E+GG+R   V+  G   Y CLK+E GVHRVQRVP T  QG+IHTST+ V +LP   +  + I   D++ E+ ++SG GGQHVNTTDSAVR+VHIPT +V  CQ+E+ Q +NK+ AMQ L+  L   + EE+ ++  + RKI IGTR RS+KIRTYNY QDR+T+HRI K+  NI+  L  +   D++I+SL E+ +++ L
Sbjct:  144 ECEDLKALASTEIASCQEEINHLKHKIASILIKREE-SEDNDLIMEVTAGVGGQEAMLFTAEMFDMYQHYAVHKNWSFDLLEY-FSSELGGLRHATVSIGGQDAYKCLKFEGGVHRVQRVPKTEKQGRIHTSTMTVAILPQPSEINLVINPKDLRIETKRASGAGGQHVNTTDSAVRIVHIPTGVVSECQQERSQIKNKEKAMQVLRAKLYTMKLEEETSKRYNARKIQIGTRGRSEKIRTYNYPQDRVTDHRIGKSLHNIEGFLFGEELLDDLIQSLSEYADYESL 428

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BLAST of Peptide chain release factor 1 vs. Ensembl Xenopus
Match: etv1 (ETS variant transcription factor 1 [Source:Xenbase;Acc:XB-GENE-489879])

HSP 1 Score: 209.92 bits (533), Expect = 1.434e-62
Identity = 131/341 (38.42%), Postives = 197/341 (57.77%), Query Frame = 1

Query:  217 EFDRINSLIQSQSLS---NRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHN--DLNEASEISMDILSG--AGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVP---ITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSL 1209
            E +R+  ++   S+     R    ++  LS L+ +  DI+    D + L     D+  E +M  L   E N L    + L  KL+  ++PH   D+N+A    +++ SG   GG     F +++ + Y+ +A  K+W FE +N +  AE GG+       SG  VY  LKYE G HRVQR+P   ++S   +IHT T+ VIVLP   +  V+I   D++ ++F+S G GGQHVNTTDSAVR+VHIPT I V CQ+E+ Q  NK+ A++ L+  L E+  E+++    S RK+ +GTR++S++IRTYN+TQDR+T+HRIH     ++NI   G    I  SL
Sbjct:   85 EHERLTEMLGQTSVREDDRRSASRRHSELSRLIGLLRDIKETEQDVRELELMCADNREEGQMLALALEEKNTLKQNINKLRGKLLQALLPHEKYDMNDAV---LEVTSGRTTGGDICQQFTKEIFDMYQNYAYYKSWSFEILNYS-PAEYGGLHHAAARISGEGVYKRLKYEGGTHRVQRIPEVGLSSRMQRIHTGTMTVIVLPQPDEVEVKIDSKDLRIDTFRSKGAGGQHVNTTDSAVRIVHIPTGIAVECQQERSQIANKEKALRILRMRLYEQTVEQELLERRSARKLQVGTRAQSERIRTYNFTQDRLTDHRIH---YEVRNIKTHGAISSIYLSL 418

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BLAST of Peptide chain release factor 1 vs. Ensembl Mouse
Match: Mtrf1l (mitochondrial translational release factor 1-like [Source:MGI Symbol;Acc:MGI:1918830])

HSP 1 Score: 223.787 bits (569), Expect = 8.874e-69
Identity = 107/266 (40.23%), Postives = 184/266 (69.17%), Query Frame = 1

Query:  445 LYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQG--YFDEIIKSLQEFDEFDQL 1236
            L  ++IS+++P  +++  S++ +++ +G GGQE+MLF  ++ + Y+++A  K W FE +   + +E+GG+R    +  G + Y  +K+E GVHRVQRVP T  QG+IHTST+ V +LP   +  + I   D++ ++ ++SG GGQHVNTTDSAVR+VH+PT I+  CQ+E+ Q +N++ AM++L+  L     EE+  +  + RKI +GT+ RS+KIRTYN+ Q+R+T+HRI+K+  ++++ + QG    D++I+SL++  +++ L
Sbjct:  103 LKHQIISLLVPSEEMD-GSDLILEVTAGVGGQEAMLFTSEMFDMYQQYAAFKRWHFETLEY-FPSELGGLRHASASVGGPEAYRHMKFEGGVHRVQRVPKTEKQGRIHTSTMTVAILPQPTEIKLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGIISECQQERSQLKNRELAMKKLRARLYSMHLEEETAKRYNARKIQVGTKGRSEKIRTYNFPQNRVTDHRINKSLHDLESFM-QGDCLLDDMIQSLKDCSDYEAL 365

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BLAST of Peptide chain release factor 1 vs. Ensembl Mouse
Match: Mtrf1 (mitochondrial translational release factor 1 [Source:MGI Symbol;Acc:MGI:2384815])

HSP 1 Score: 194.897 bits (494), Expect = 5.692e-57
Identity = 126/364 (34.62%), Postives = 214/364 (58.79%), Query Frame = 1

Query:  163 MPKYLKDELINFQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVN--VDE-QYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSG--AGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVP---ITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNIL-NQGYFDEII-KSLQEFDE 1224
            + KY++D      N++    D+    I       R L  ++ +L+ L  ++ +I+     ++E + L  SL   D E ++ +L+  E   +  +   LYS+L+  ++P    +  S++ +++ SG   GG     F  ++ + Y+ ++  K+W FE +N    A+ GG+       SG+ VY  LKYE G+HRVQR+P   ++S   +IHT T++VIVLP   +  V++   D++ ++F++ G GGQHVNTTDSAVRLVHIPT +VV CQ+E+ Q +NK+ A++ L+  L ++  E+   +  + RK+ +GTR++S++IRTYN+TQDR+T+HRI     +I+  L  +   D++I + LQ  DE
Sbjct:   76 LQKYMED-----LNKEYQTLDQCLQGISENEGDRRALHRRHAQLAPLAAVYQEIQEAEQAIEELESLCKSLNKQD-EKQLQELVSEERQIIDQKIHRLYSELLERLVPKEKYD-WSDVILEVTSGRTTGGDICQQFTREIFDMYQNYSYYKHWKFELLNYTP-ADYGGLHHAAARISGDSVYKHLKYEGGIHRVQRIPEVGLSSRMQRIHTGTMSVIVLPQPDEVDVKVDPKDLRVDTFRARGAGGQHVNTTDSAVRLVHIPTGLVVECQQERSQLKNKEIALRVLRARLYQQIIEKDRCQQQNARKLQVGTRAQSERIRTYNFTQDRVTDHRIAYEVRDIKEFLRGEKCLDQLIERLLQSADE 431

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BLAST of Peptide chain release factor 1 vs. Ensembl Mouse
Match: 2810006K23Rik (RIKEN cDNA 2810006K23 gene [Source:MGI Symbol;Acc:MGI:1919900])

HSP 1 Score: 48.521 bits (114), Expect = 2.771e-6
Identity = 23/60 (38.33%), Postives = 37/60 (61.67%), Query Frame = 1

Query:  823 IKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKD 1002
            + ES+++ +  K  GPGGQ  N T + V L H+P+ IVV C + +   QN+K A + L++
Sbjct:   58 LNESELEEQFVKGHGPGGQATNKTSNCVVLKHVPSGIVVKCHQTRSVDQNRKIARKVLQE 117

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BLAST of Peptide chain release factor 1 vs. UniProt/SwissProt
Match: sp|Q3A928|RF1_CARHZ (Peptide chain release factor 1 OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=prfA PE=3 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 2.864e-72
Identity = 138/353 (39.09%), Postives = 212/353 (60.06%), Query Frame = 1

Query:  220 FDRINSLIQ-----SQSLSNRDLLEQYGRLSSLVDIFNDIE-LVNVDEQYLNN--------SLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSLQEFDEFDQL 1236
            FD+++ L +     S+ +S+ +++    R    V    +IE +V V  +Y            +LD   +P+  +L++ E + L  RR+ L +KL  +++P  D N+   + M+I +GAGG+E+ LFA DL   Y K+A    W  E ++ ++  ++GG +       G  VY+ +K+ESGVHRVQRVP T S G+IHTST  V VLP   +  VEI  +D++ + F +SG GGQHVN T+SAVR+ HIPT IVV+CQ EK Q +NK+ AM+ L+  L++K  +E+   L S RK  +GT  RS++IRTYN+ Q+R+T+HRI      +  +L +G  DEII +L   D+ ++L
Sbjct:    2 FDKLDQLEEKYEELSRLISDPEVIADTQRWQGYVKAHAEIEEIVQVYREYKKTVKEIEDAERMLDEKLDPDFRELVQSELHDLKERREELEAKLKILLLP-KDPNDEKNVIMEIRAGAGGEEAALFAGDLFRMYSKYADRMGWKVEIMD-SHPTDLGGFKEVIFTIEGKGVYSRMKFESGVHRVQRVPTTESGGRIHTSTATVAVLPEAEEVEVEINPNDLRIDVFCASGAGGQHVNKTESAVRITHIPTGIVVTCQDEKSQHKNKERAMKILRARLLDKARQEQEAELASARKSQVGTGDRSERIRTYNFPQNRVTDHRIGLTLHRLDAVL-EGDLDEIIDALITTDQAERL 351

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BLAST of Peptide chain release factor 1 vs. UniProt/SwissProt
Match: sp|Q3A129|RF1_PELCD (Peptide chain release factor 1 OS=Pelobacter carbinolicus (strain DSM 2380 / NBRC 103641 / GraBd1) OX=338963 GN=prfA PE=3 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 4.246e-71
Identity = 125/314 (39.81%), Postives = 194/314 (61.78%), Query Frame = 1

Query:  268 DLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSL 1209
            +L  ++  LSS+V ++   E   V E    N  L  D++PEM ++ + E  +L A R+ L  +L  +++P  D N+   + ++I +G GG E+ LFA DL   Y +FA  + W  E ++++  +E GG +      SGN+VY+ LKYESG HRVQRVP T +QG+IHTS   V VLP   D  V+I  +D++ + +++SG GGQHVN T+SAVR+ H+PT +VVSCQ EK Q +NK  AM+ LK  ++++   ++  ++ + RK  +G+  RS +IRTYN+ Q R T+HRI      ++ I+ QG   E+++ L
Sbjct:   34 ELTREHAELSSVVAVYR--EYKRVSEDIEGNRELLQDSDPEMREMAKAELPELEAHREELAQQLKVLLLP-KDPNDDKNVILEIRAGTGGDEAALFAGDLFRMYSRFAEGQGWKVETMSVSD-SEAGGFKEIIAMISGNRVYSQLKYESGTHRVQRVPETEAQGRIHTSACTVAVLPEAEDVDVDIDPTDLRIDVYRASGAGGQHVNKTESAVRITHVPTGVVVSCQDEKSQHKNKAKAMKVLKSRILDQVMADQQAQMAADRKSQVGSGDRSQRIRTYNFPQGRCTDHRIGLTLYRLEGIM-QGNLSELVEPL 342

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BLAST of Peptide chain release factor 1 vs. UniProt/SwissProt
Match: sp|Q9K6F4|RF1_BACHD (Peptide chain release factor 1 OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=prfA PE=3 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 5.856e-71
Identity = 132/335 (39.40%), Postives = 205/335 (61.19%), Query Frame = 1

Query:  220 FDRINSLIQ-SQSLSNRDLLEQYGR----LSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSL 1209
            ++R+N L+   + +S+ + L +Y +    +   V ++ + + V +D+      +L+   + EM  +++ E ++L+ R++ L +KL  +++P  D N+   + +++   AGG E+ LFA DL   Y ++A A+ W  E +  +   E+GG +    + +G   Y+ LKYE+G HRVQRVP T S G+IHTST  V VLP   +  V+I E DI+ ++F SSGPGGQ VNTT SAVRL H+PT IVVSCQ EK Q +NK+ AM+ L+  + +K  +E     +  RK  +GT  RS++IRTYN+ Q R+T+HRI      ++ IL QG  DEII+SL
Sbjct:   12 YERLNELLSDPEVISDSNKLREYSKEQADMEETVQVYREYKTV-MDQYQDAKEMLEDKLDDEMYAMVKEEIDELATRKEDLEAKLKVLLLP-KDPNDDKNVIVEVRGAAGGDEAQLFAADLYKMYARYAEAQGWKIEVIEAS-STELGGYKEIIFSVNGKGAYSKLKYENGAHRVQRVPQTESGGRIHTSTATVAVLPEAEEVEVDIHEKDIRVDTFASSGPGGQSVNTTMSAVRLTHLPTGIVVSCQDEKSQIKNKEKAMKVLRARIYDKFQQEAQAEYDQTRKSAVGTGDRSERIRTYNFPQSRVTDHRIGLTLQKLEQIL-QGKLDEIIESL 342

[Close]

BLAST of Peptide chain release factor 1 vs. UniProt/SwissProt
Match: sp|Q4V7E5|RF1ML_RAT (Peptide chain release factor 1-like, mitochondrial OS=Rattus norvegicus OX=10116 GN=Mtrf1l PE=2 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 9.632e-71
Identity = 109/266 (40.98%), Postives = 186/266 (69.92%), Query Frame = 1

Query:  445 LYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQG--YFDEIIKSLQEFDEFDQL 1236
            L  ++IS+++P  D++  S++ +++ +G GGQE+MLF  ++ + Y+++A  K W FE +   + +E+GG+R    +  G + Y  +K+E GVHRVQRVP T  QG+IHTST+ V +LP   +  + I   D++ ++ ++SG GGQHVNTTDSAVR+VH+PT I+  CQ+E+ Q +N++ AM++L+  L   + EE+  +  S RKI +GT+ RS+KIRTYN+ Q+R+T+HRI+K+  ++++ + QG    D++I+SL+++ +++ L
Sbjct:  103 LKHRIISLLVPSEDMD-GSDLILEVTAGVGGQEAMLFTSEMFDMYQQYAAFKRWHFETLEY-FPSELGGLRHASASIGGPEAYRHMKFEGGVHRVQRVPKTERQGRIHTSTMTVAILPQPTEIKLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGIISECQQERSQLKNRELAMKKLRARLYSMRLEEETAKRYSARKIQVGTKGRSEKIRTYNFPQNRVTDHRINKSLHDLESFM-QGDCLLDDLIQSLKDYSDYESL 365

[Close]

BLAST of Peptide chain release factor 1 vs. UniProt/SwissProt
Match: sp|Q9UGC7|RF1ML_HUMAN (Peptide chain release factor 1-like, mitochondrial OS=Homo sapiens OX=9606 GN=MTRF1L PE=1 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 1.238e-70
Identity = 119/316 (37.66%), Postives = 207/316 (65.51%), Query Frame = 1

Query:  307 DIFNDIELVNVDEQYLNNS--LLDSDTEPEMADLMRLESNQLSARRDA---LYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGY-FDEIIKSLQEFDEFDQL 1236
            ++   I+L+N  E+ L  +  LL  + E    DL +L  N+++  +     L  ++I +++P  + +E +++ +++ +G GGQE+MLF  ++ + Y+++A  K W FE +   + +E+GG+R    +  G++ Y  +K+E GVHRVQRVP T  QG++HTST+ V +LP   +  + I   D++ ++ ++SG GGQHVNTTDSAVR+VH+PT +V  CQ+E+ Q +NK+ AM +L+  L     EE++N+  + RKI IG++ RS+KIRTYN+ Q+R+T+HRI+K   +++  +   Y  DE+++SL+E+ +++ L
Sbjct:   63 ELLAVIKLLNEKERELRETEHLLHDENE----DLRKLAENEITLCQKEITQLKHQIILLLVPSEETDE-NDLILEVTAGVGGQEAMLFTSEIFDMYQQYAAFKRWHFETLEY-FPSELGGLRHASASIGGSEAYRHMKFEGGVHRVQRVPKTEKQGRVHTSTMTVAILPQPTEINLVINPKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKRQNARKIQIGSKGRSEKIRTYNFPQNRVTDHRINKTLHDLETFMQGDYLLDELVQSLKEYADYESL 372

[Close]

BLAST of Peptide chain release factor 1 vs. TrEMBL
Match: V9KT85 (Peptide chain release factor 1-like, mitochondrial OS=Callorhinchus milii OX=7868 PE=2 SV=1)

HSP 1 Score: 261.151 bits (666), Expect = 5.114e-79
Identity = 147/348 (42.24%), Postives = 222/348 (63.79%), Query Frame = 1

Query:  241 IQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRL-ESNQLSARRD--ALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQN-ILNQGYFDEIIKSLQEFDEFDQLELALRNSTNCKQ 1272
            +QS   S  ++     R   L  +  ++  + V  Q L +  +++  + E ADL +L ES+  S + D  AL  K+IS+V P  + +E S++ +++L+G GGQE+MLF  D+ N Y+ +AT K+W FE +   + +E+GG+R    + SG +VY  LKYE GVHRVQRVP T  QG++HTST+ V +LP   +  + I   D++ E+ ++SG GGQHVNTTDSAVR++HIPT  V  CQ+E+ Q +NK  AMQ L+  L   + EE+  + +  RKI IGT+ R++KIRTYN+ QDRIT+HRI K+  ++++ +L +G  DE+I+ L EF + + L   L N T+ KQ
Sbjct:   88 LQSAQASEEEVKGAGKRARELAPLVTELHGLRVKVQELED--IETLMKDEDADLRKLAESDWNSCQEDIRALKHKIISLVTPSEETDE-SDLILEVLAGVGGQEAMLFTADVFNMYRSYATYKSWHFETLEY-FPSELGGLRHATASISGREVYRHLKYEGGVHRVQRVPKTERQGRLHTSTMTVALLPQPSEIHLTINPKDLRIETKRASGAGGQHVNTTDSAVRILHIPTGFVAECQQERSQLKNKSKAMQLLRAKLYSARLEEETTKRSYARKIQIGTKGRAEKIRTYNFPQDRITDHRIGKSLHDMRSFLLGEGSLDEMIQDLMEFADTESLLELLDNDTDSKQ 431

[Close]

BLAST of Peptide chain release factor 1 vs. TrEMBL
Match: A0A4W3I595 (RF_PROK_I domain-containing protein OS=Callorhinchus milii OX=7868 PE=4 SV=1)

HSP 1 Score: 261.151 bits (666), Expect = 6.042e-79
Identity = 147/348 (42.24%), Postives = 222/348 (63.79%), Query Frame = 1

Query:  241 IQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRL-ESNQLSARRD--ALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQN-ILNQGYFDEIIKSLQEFDEFDQLELALRNSTNCKQ 1272
            +QS   S  ++     R   L  +  ++  + V  Q L +  +++  + E ADL +L ES+  S + D  AL  K+IS+V P  + +E S++ +++L+G GGQE+MLF  D+ N Y+ +AT K+W FE +   + +E+GG+R    + SG +VY  LKYE GVHRVQRVP T  QG++HTST+ V +LP   +  + I   D++ E+ ++SG GGQHVNTTDSAVR++HIPT  V  CQ+E+ Q +NK  AMQ L+  L   + EE+  + +  RKI IGT+ R++KIRTYN+ QDRIT+HRI K+  ++++ +L +G  DE+I+ L EF + + L   L N T+ KQ
Sbjct:   99 LQSAQASEEEVKGAGKRARELAPLVTELHGLRVKVQELED--IETLMKDEDADLRKLAESDWNSCQEDIRALKHKIISLVTPSEETDE-SDLILEVLAGVGGQEAMLFTADVFNMYRSYATYKSWHFETLEY-FPSELGGLRHATASISGREVYRHLKYEGGVHRVQRVPKTERQGRLHTSTMTVALLPQPSEIHLTINPKDLRIETKRASGAGGQHVNTTDSAVRILHIPTGFVAECQQERSQLKNKSKAMQLLRAKLYSARLEEETTKRSYARKIQIGTKGRAEKIRTYNFPQDRITDHRIGKSLHDMRSFLLGEGSLDEMIQDLMEFADTESLLELLDNDTDSKQ 442

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BLAST of Peptide chain release factor 1 vs. TrEMBL
Match: A0A0G0IRH8 (Peptide chain release factor 1 OS=Parcubacteria group bacterium GW2011_GWD2_38_11 OX=1618941 GN=prfA PE=3 SV=1)

HSP 1 Score: 249.98 bits (637), Expect = 7.783e-76
Identity = 149/355 (41.97%), Postives = 214/355 (60.28%), Query Frame = 1

Query:  217 EFDRINSLIQSQSL-SNRDLLEQYGR----LSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSG---NQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSLQEFDEFDQLELALRNS 1257
            E+D I + + S  + +N   + Q G+    L  L+   N+ E +   E   N  ++++D + EM ++   E+ QL+ R+  +  +L  M++P  D N+   I ++I  GAGG ES LFA  L   Y KFA   NW  + +N N   E+GG +      SG   N VY  +K+ESGVHRVQR+P T  QG+IHTST  V VL    +  + I E+D++ ++F SSGPGGQ VNTT SAVR+ HIPT +VVSCQ EK Q +NK  AM+ L+  L++ + E K   L   RK  +GT  RS+KIRTYNY QDRIT+HRI +++SN++ IL+ G  D II +++E D    ++L + NS
Sbjct:   11 EYDEITAELSSPDIFNNAKKMAQLGKRQSELQGLMQKINEYEKIQ-QEMKGNAEIMNTDEDAEMKEMATTENIQLAQRKAEIEKELEIMLLPK-DPNDDKNIIVEIRGGAGGDESALFAASLFRMYSKFAEKNNWKLDVLNSNRT-ELGGFKEVVFEISGTPSNPVYQKMKFESGVHRVQRIPETEKQGRIHTSTATVAVLAEAEEVDLVISENDLRIDTFCSSGPGGQSVNTTKSAVRITHIPTGVVVSCQDEKSQLKNKDKAMKVLRSRLLQAEEERKAKELGDARKSQVGTGDRSEKIRTYNYPQDRITDHRIKQSWSNMEGILD-GNIDPIIDAIREED----IKLKMSNS 357

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BLAST of Peptide chain release factor 1 vs. TrEMBL
Match: A1ZI31 (Peptide chain release factor 1 OS=Microscilla marina ATCC 23134 OX=313606 GN=prfA PE=3 SV=1)

HSP 1 Score: 248.054 bits (632), Expect = 5.404e-75
Identity = 139/346 (40.17%), Postives = 219/346 (63.29%), Query Frame = 1

Query:  220 FDRINSLI-QSQSLSNRDL----LEQYGRLSSLVDIFNDIELV--NVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSLQEFDEFDQL 1236
            FD +  LI + +++ + DL     ++Y  L  +V+ +N+ ++V  N+D    +  +L ++ +PEM ++ ++E  +L+ +++ +  +L +++IP  D N++  + ++I  GAGG E+ +FA DLL  Y++FA  + W FE ++    +  GG +    + SG  VY  +KYESGVHRVQRVP T +QG++HTS  +V VLP + D  V I  SD++ + F SSGPGGQ VNTT SAVRL HIPT IVVSCQ EK Q +NK  AM+ L+  L E +  ++   L + RK ++G+  RSDKIRTYNY Q R+T+HRI     N+ N+++ G   + I+SL+  D  +++
Sbjct:   12 FDEVGQLIVKPETIQDMDLYTKLTKEYKDLEKVVEKYNEYKIVLENID---TSKEILSTEKDPEMKEMAKMELEELNPQKEKIEEELKTLLIP-KDPNDSKNVILEIRGGAGGDEAAIFAGDLLRMYQRFAEKRKWKFELMDFTDGSS-GGFKEVVCSISGEDVYGMMKYESGVHRVQRVPATETQGRVHTSAASVAVLPEMEDVEVNIDPSDVRSDYFCSSGPGGQSVNTTYSAVRLTHIPTGIVVSCQDEKSQIKNKAKAMKVLRSKLYEIELSKQQEELGAQRKSMVGSGDRSDKIRTYNYPQGRVTDHRIKYTVHNLPNVMD-GEIADFIESLRLADNAEKM 351

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BLAST of Peptide chain release factor 1 vs. TrEMBL
Match: K1ZC72 (PCRF domain-containing protein OS=uncultured bacterium OX=77133 GN=ACD_67C00091G0001 PE=4 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 7.759e-75
Identity = 135/292 (46.23%), Postives = 185/292 (63.36%), Query Frame = 1

Query:  355 NNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSG---NQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSLQEFD 1221
            N  ++++D +PEM ++   E+  LS R+  +  +L  M++P  D N+   I ++I  GAGG ES LFA  L   Y KFA   NW    +N N   E+GG +      +G   N VY  +K+ESGVHRVQR+P T  QG+IHTST  V VL    +  + I ++D++ ++F SSGPGGQ VNTT SAVR+ HIPT +VVSCQ EK Q +NK+ AM+ L+  L++ + E K   L   RK  +GT  RS+KIRTYNY QDRIT+HRI +++SNI+ IL  G  D II +L+E D
Sbjct:   17 NAEIMNADEDPEMKEMATEENIGLSKRKAEIEKELEIMLLPK-DPNDNKNIIVEIRGGAGGDESALFAASLFRMYSKFAEKNNWKLGVLNSNRT-ELGGFKEVVFEITGTASNPVYQKMKFESGVHRVQRIPETEKQGRIHTSTATVAVLAEAEEVDLTINDNDLRIDTFCSSGPGGQSVNTTKSAVRITHIPTGVVVSCQDEKSQLKNKEKAMKVLRSRLLQAEEERKAKELGDARKSQVGTGDRSEKIRTYNYPQDRITDHRIKQSWSNIEGILG-GDIDAIIDALKEED 305

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BLAST of Peptide chain release factor 1 vs. Ensembl Cavefish
Match: mtrf1l (mitochondrial translational release factor 1-like [Source:ZFIN;Acc:ZDB-GENE-030131-8198])

HSP 1 Score: 210.305 bits (534), Expect = 4.899e-63
Identity = 111/261 (42.53%), Postives = 170/261 (65.13%), Query Frame = 1

Query:  454 KLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQN-ILNQGYFDEIIKSLQEFDEFDQ 1233
            ++IS +IP  + ++ S++ +++ +G GGQE+MLF  ++   Y+ FA+   W FE +     +++GG+R    + SG   Y  +K+E+GVHRVQRVP T  QG++HTST+ V +LP   +    I   D++ E+ ++SG GGQHVNTT+SAVR+VH+PT IV   Q+E+ Q  NK+ AM+ L   L   + EE+ ++    RK+ IGT+ RS+KIRTYN+ QDRIT+HRI K   ++   +L +   DE+  SLQ+F  +DQ
Sbjct:  161 QIISALIPEEE-SDMSDLVLEVTAGIGGQEAMLFTAEIFTMYQNFASFHGWGFEILEF-MSSDIGGVRHATASISGPLSYKKMKFEAGVHRVQRVPKTEKQGRMHTSTMTVAILPQPAEISFTINPKDLRIETKRASGAGGQHVNTTNSAVRIVHLPTGIVSESQQERSQIINKEKAMKVLLAKLYSAKLEEETSKRLQARKLQIGTKGRSEKIRTYNFCQDRITDHRIGKTLHDLDAFLLGEDLLDEMSSSLQQF--YDQ 417

[Close]

BLAST of Peptide chain release factor 1 vs. Ensembl Cavefish
Match: mtrf1l (mitochondrial translational release factor 1-like [Source:ZFIN;Acc:ZDB-GENE-030131-8198])

HSP 1 Score: 189.889 bits (481), Expect = 2.173e-55
Identity = 104/260 (40.00%), Postives = 161/260 (61.92%), Query Frame = 1

Query:  454 KLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSLQEFDEFDQ 1233
            ++IS +IP  + ++ S++ +++ +G GGQE+MLF  ++   Y+ FA+   W FE +     +++GG+R    + SG   Y  +K+E+GVHRVQRVP T  QG++HTST+ V +          + +     E+ ++SG GGQHVNTT+SAVR+VH+PT IV   Q+E+ Q  NK+ AM+ L   L   + EE+ ++    RK+ IGT+ RS+KIRTYN+ QDRIT+HRI K   ++          ++  SLQ+F  +DQ
Sbjct:  168 QIISALIPEEE-SDMSDLVLEVTAGIGGQEAMLFTAEIFTMYQNFASFHGWGFEILEF-MSSDIGGVRHATASISGPLSYKKMKFEAGVHRVQRVPKTEKQGRMHTSTMTVAI----------LPQPAEVIETKRASGAGGQHVNTTNSAVRIVHLPTGIVSESQQERSQIINKEKAMKVLLAKLYSAKLEEETSKRLQARKLQIGTKGRSEKIRTYNFCQDRITDHRIGKTLHDL----------DMSSSLQQF--YDQ 403

[Close]

BLAST of Peptide chain release factor 1 vs. Ensembl Cavefish
Match: mtrf1 (mitochondrial translational release factor 1 [Source:ZFIN;Acc:ZDB-GENE-050220-8])

HSP 1 Score: 161.77 bits (408), Expect = 8.599e-47
Identity = 87/209 (41.63%), Postives = 133/209 (63.64%), Query Frame = 1

Query:  565 LLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVP---ITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQG 1182
            + + Y+ FA+ K+W FE  N    A+ GG+    V  SG  VY  LK+E G HRVQR+P   ++S   +IHT T+ VIVLP   D  + I   D++ ++F+S G GGQ VNTTDSAVR+VH+PT  V  CQ+ + Q QN++ AM+ LK  L +     ++   ++ RK  +GTR++S++IR+Y+++QDR+T+HRI     +I+  ++ G
Sbjct:    1 VFDMYQGFASYKDWDFEVFNYTE-ADYGGLHHAAVKISGENVYRWLKFEGGTHRVQRIPEVGLSSRMQRIHTGTMMVIVLPQPSDVDIAIDPKDLRIDTFRSRGAGGQSVNTTDSAVRIVHLPTGTVAECQQSRSQLQNRELAMRVLKARLYQSTVGRQVEESHTARKQQVGTRAQSERIRSYHFSQDRVTDHRIGYVSRDIKEFMSGG 208

[Close]

BLAST of Peptide chain release factor 1 vs. Ensembl Sea Lamprey
Match: mtrf1l (mitochondrial translational release factor 1-like [Source:ZFIN;Acc:ZDB-GENE-030131-8198])

HSP 1 Score: 208.379 bits (529), Expect = 2.491e-64
Identity = 113/294 (38.44%), Postives = 178/294 (60.54%), Query Frame = 1

Query:  358 NSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQG-YFDEIIKSLQEFDEFDQL 1236
            +SLL  D + EM  L   E+    A+   L  K++++ +   +  +  E+ +++ +G GGQE+MLF  ++   Y+ +A  +NW FE +   + +EMGG+R      +G   Y  L +E GVHRVQR+P T S+G++HTST+ V VL    +  + I   +++ E+ ++SG GGQ VNTTDS+VR+ H+PT +V  CQKE+ Q QN K AM  L+  L + Q EE++++  + RK+ +G+R RS+KIRTYN+ QDRIT+HRI +    +   L  G     ++ +LQE    + L
Sbjct:   23 DSLLH-DCDEEMRKLAEEENENCKAKIHELQEKVLAL-LLPGEEEDEGELLLEVYAGVGGQEAMLFTAEVFEMYENYALHQNWCFERLQYQW-SEMGGLRHATACITGEGAYRLLCFEGGVHRVQRIPRTESKGRVHTSTITVAVLRQPSEIELNISPKELRIETKRASGAGGQSVNTTDSSVRITHLPTGVVSECQKERSQIQNLKQAMTILRAKLYDLQLEERLSKQQTARKLQVGSRGRSEKIRTYNFMQDRITDHRIGRTVHGVAAFLEGGEALHHMVAALQEVARSEAL 313

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BLAST of Peptide chain release factor 1 vs. Ensembl Sea Lamprey
Match: mtrf1 (mitochondrial translational release factor 1 [Source:ZFIN;Acc:ZDB-GENE-050220-8])

HSP 1 Score: 159.844 bits (403), Expect = 1.598e-45
Identity = 97/276 (35.14%), Postives = 169/276 (61.23%), Query Frame = 1

Query:  367 LDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSG--AGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVP---ITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQH-----VNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQ 1164
            ++++ +  M  L   E  ++      L  K++ +++P  D ++  ++ +++ +G   GG     FA +L + Y+ FA  +NW FE ++    A+ GG+       +G+ V+  LK+E G HRVQR+P   ++S   +IHT T  VIVLP   D  ++++  D++ ++F++ G GGQH     VNTTDSAVR+VH+PT +   CQ+E+ Q +NK+ A+Q LK  + ++ +E ++ + +S R++ +GTRS+S++IRTY  TQDR+T+HRI     ++Q
Sbjct:   76 VEAEKDRHMQALAVEEKERIQGEAGNLERKVLDLLLP-QDPHDDRDVILEVTAGRTTGGDICQRFAAELFDMYQGFAKHRNWDFEIMSFAL-ADCGGLHHASARITGDAVFRHLKFEGGTHRVQRIPQTGLSSRMERIHTGTTTVIVLPQPRDIDIKLETKDLRIDTFRAKGAGGQHAGGQHVNTTDSAVRVVHLPTGLTAECQQERSQIRNKEMALQILKTKMYQQLFELEVQKRHSARRLQVGTRSQSERIRTYTLTQDRVTDHRISLTLHDVQ 349

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BLAST of Peptide chain release factor 1 vs. Ensembl Sea Lamprey
Match: si:ch211-275j6.5 (chromosome 12 open reading frame 65 [Source:HGNC Symbol;Acc:HGNC:26784])

HSP 1 Score: 50.8322 bits (120), Expect = 1.808e-8
Identity = 27/83 (32.53%), Postives = 46/83 (55.42%), Query Frame = 1

Query:  823 IKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEE----KMNRLNSHRK 1059
            ++E D++ +  + SGPGGQ  N T + V L H+P+ IVV C + +  + N+K A   L+  L E  + +    ++ RL   R+
Sbjct:    1 LREEDLREQFVRGSGPGGQATNKTSNCVVLKHVPSGIVVKCHQTRSLETNRKLARGILRGKLDEHMHGDASAVRLQRLEGQRQ 83

[Close]

BLAST of Peptide chain release factor 1 vs. Ensembl Yeast
Match: MRF1 (Mitochondrial translation release factor; involved in stop codon recognition and hydrolysis of the peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability [Source:SGD;Acc:S000003111])

HSP 1 Score: 174.481 bits (441), Expect = 1.459e-50
Identity = 113/328 (34.45%), Postives = 190/328 (57.93%), Query Frame = 1

Query:  277 EQYGRLSSLVDIFNDI-----ELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXE--------IKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSLQEFD 1221
            + Y +LS+L D F +      EL ++ E  +++  L ++ E E A+L+         + +   S+L++ ++P +   +   + +++  G GG E+M+F ++LL+ Y  +A  + W +  ++ N      GI    ++      Y+ L++E+GVHRVQR+P T ++G+ HTST AV+VLP + D+  +         K  +I+ +  ++SG GGQHVNTTDSAVRL HIP+ IVVS Q E+ Q +NK  A   L+  L EK+  EK  +    RK  + + +RSDKIRTYN+ Q+RIT+HR      ++  +L+    DE+I+++ ++D
Sbjct:   83 KHYAKLSALTDTFIEYKEKLNELKSLQEMIVSDPSLRAEAEQEYAELV--------PQYETTSSRLVNKLLPPHPFADKPSL-LELRPGVGGIEAMIFTQNLLDMYIGYANYRKWKYRIISKNENESGSGIIDAILSIEEAGSYDRLRFEAGVHRVQRIPSTETKGRTHTSTAAVVVLPQIGDESAKSIDAYERTFKPGEIRVDIMRASGKGGQHVNTTDSAVRLTHIPSGIVVSMQDERSQHKNKAKAFTILRARLAEKERLEKEEKERKARKSQVSSTNRSDKIRTYNFPQNRITDHRCGFTLLDLPGVLSGERLDEVIEAMSKYD 401

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BLAST of Peptide chain release factor 1 vs. Ensembl Yeast
Match: YLR281C_mRNA (Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YLR281C is not an essential gene [Source:SGD;Acc:S000004271])

HSP 1 Score: 50.0618 bits (118), Expect = 5.968e-8
Identity = 22/44 (50.00%), Postives = 31/44 (70.45%), Query Frame = 1

Query:  865 GPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQL 996
            GPGGQ +N  +S V+L H PT IVV CQ+ + ++QN+K A  +L
Sbjct:   47 GPGGQKINKCNSKVQLRHEPTGIVVECQETRSREQNRKLARLKL 90

[Close]

BLAST of Peptide chain release factor 1 vs. Ensembl Nematostella
Match: EDO42717 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S0R2])

HSP 1 Score: 209.92 bits (533), Expect = 3.414e-65
Identity = 116/274 (42.34%), Postives = 175/274 (63.87%), Query Frame = 1

Query:  457 LISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGY-FDEIIKSLQEFDEFDQL-ELALRNSTNCKQ 1272
            ++  +IP ++ +E   I +++ +G GGQE+ LFA ++   Y++FA  K W F+ ++I   ++ GG +    + SG  V+  +K+E GVHRVQRVP+T +QG++HTST+ V VLP   +  V I  +D+K ++F++SG GGQHVNTTDSAVR+ HIP+ +VVS Q+E+ Q +NK  AMQ L+  L + Q +E  +     R+  IGT  RS+KIRTYN+ Q R+T+HRI      +   L  G   +++I +L+  DE D L EL  R +TN  Q
Sbjct:    1 IMKALIPKDEADENDAI-LEVRAGTGGQEASLFASEMFIMYQRFAAFKKWKFDILDIAR-SDTGGYKEASASISGYGVFGKMKFEVGVHRVQRVPVTEAQGRVHTSTMTVAVLPQPQEVDVIISPNDLKIDTFRASGAGGQHVNTTDSAVRITHIPSGMVVSSQEERSQFKNKAKAMQVLRARLYDLQRQEADSARVHARRTQIGTAERSEKIRTYNFPQGRVTDHRIGMTSHTVDKFLEGGVLLEDMIDALRVQDERDALKELVDRYTTNINQ 272

[Close]

BLAST of Peptide chain release factor 1 vs. Ensembl Medaka
Match: mtrf1l (mitochondrial translational release factor 1 like [Source:NCBI gene;Acc:101157560])

HSP 1 Score: 220.32 bits (560), Expect = 6.711e-67
Identity = 125/352 (35.51%), Postives = 205/352 (58.24%), Query Frame = 1

Query:  169 KYLKDELINFQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDI-ELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQN-ILNQGYFDEIIKSLQEF 1218
            K L   L   Q E       I+S+      S  +L  +  ++S L  +   I EL    E+     +L  + +  + +L  LE          L +K++ ++IP   + E S++ +++ +G GGQE+MLF  ++   Y+ FA    W F+ +  +  +++GG+R    + SG Q Y  +K+E+GVHRVQRVP T  QG++HTST+ V VLP   +    I + D++ ++ ++SG GGQHVNTTDSAVR+VH+PT +V  CQ+E+ Q +N++ AM+ L+  L   + EE+ ++  + RKI +GT+ RS+KIRTYN+ QDRIT+HRI     ++++ +L +   +++   LQE 
Sbjct:   65 KSLHQHLTKVQLEYKECLQAISSITMEDQCSEEELRAKRTKISLLAPLIQSIRELDKKHEEMAETEMLLKEEDQALRELAELEKEGCLQDIQQLRAKILELLIPEERV-ELSDLVLEVTAGVGGQEAMLFTAEVFEMYQGFAQYHGWSFDILE-HMTSDLGGLRHASASISGPQSYKKMKFEAGVHRVQRVPKTEKQGRMHTSTMTVAVLPQPTEISFTINQKDLRIDTKRASGAGGQHVNTTDSAVRIVHLPTGVVAECQQERSQLKNREKAMKALRAKLYSMKLEEETSKRYNQRKIQVGTKGRSEKIRTYNFQQDRITDHRISITVHDVKSFLLGEDLLEDMNSLLQEL 414

[Close]

BLAST of Peptide chain release factor 1 vs. Ensembl Medaka
Match: mtrf1 (mitochondrial translation release factor 1 [Source:NCBI gene;Acc:101161709])

HSP 1 Score: 209.149 bits (531), Expect = 1.504e-62
Identity = 140/429 (32.63%), Postives = 231/429 (53.85%), Query Frame = 1

Query:   22 CPICFSI----------YITF*LIKMNFINKFLKTSQLFNNFRFIDYKTFTNISYINMPKYLKDELIN----FQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQ----YLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSG--AGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVP---ITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQG-YFDEIIKSLQEFDEFDQL 1236
            C +C ++          + T  L++   I     T + F++  F+D   + N S     K L DE  +     Q+  L+E DR            +  ++++  L  L ++F   E    D +     L+++    D +  +  L+R E  Q+++R  +L   LI +++P   L+ +S++ ++++SG   GG     F  ++ + Y+ FA  KNW F+ +N    AE GG+    V  +G  VY  LK+E G HRVQR+P   ++S   +IHT T+ VIVLP   D  + I+  D++ ++F+S G GGQ VNTTDSAVR+VH+PT I   CQ+ + Q QN++TAMQ L+  L +    ++  + ++ RK  +GTRS+S++IRTYN++QDR+T+HR   +  +I+  +  G   DE+I  L E  E + L
Sbjct:   10 CSLCSTLISRSGRSRGGWRTLLLLQQTSIRHSEVTPKRFSHGDFLDL--YENESVQKYVKQLMDEYTDISQKLQHAHLSESDR------------KAFIKKHAELVPLANVFKRTEQAKKDLEEVLSVLHSTTATEDEDEHLIQLLREEETQMTSRILSLKKDLIKVLVPTEPLD-SSDVVLEVVSGRTTGGDICQQFTREMFDMYQGFACYKNWDFKLLNYT-PAEYGGLHHAAVRIAGESVYRHLKHEGGTHRVQRIPEVGLSSRMQRIHTGTMTVIVLPQPKDLNLHIEPKDLRVDTFRSRGAGGQSVNTTDSAVRIVHLPTGITAECQQTRSQLQNRETAMQMLRVRLYQSMMGKETEQRHTARKQQVGTRSQSERIRTYNFSQDRVTDHRTGYSTRDIKEFMKGGEELDELIADLLEHVEREAL 422

[Close]

BLAST of Peptide chain release factor 1 vs. Ensembl Medaka
Match: si:ch211-275j6.5 (chromosome 9 C12orf65 homolog [Source:NCBI gene;Acc:101159678])

HSP 1 Score: 46.9802 bits (110), Expect = 6.979e-6
Identity = 23/60 (38.33%), Postives = 36/60 (60.00%), Query Frame = 1

Query:  829 ESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLL 1008
            E +++ +  + SGPGGQ  N T + V L HIPT IVV C + +    N+K A + +++ L
Sbjct:   54 EDELEEQFVRGSGPGGQATNKTSNCVVLKHIPTGIVVKCHQTRSVDINRKRAREIMREKL 113

[Close]

BLAST of Peptide chain release factor 1 vs. Planmine SMEST
Match: SMESG000022027.1 (SMESG000022027.1)

HSP 1 Score: 796.964 bits (2057), Expect = 0.000e+0
Identity = 404/405 (99.75%), Postives = 405/405 (100.00%), Query Frame = 1

Query:   67 MNFINKFLKTSQLFNNFRFIDYKTFTNISYINMPKYLKDELINFQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSLQEFDEFDQLELALRNSTNCKQFIQ 1281
            MNFINKFLKTSQLFNNFRFIDYKTFTNISYINMPKYLKDELINFQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTVAVIVLPVLPDDVVEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIK+LQEFDEFDQLELALRNSTNCKQFIQ
Sbjct:    1 MNFINKFLKTSQLFNNFRFIDYKTFTNISYINMPKYLKDELINFQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTVAVIVLPVLPDDVVEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKNLQEFDEFDQLELALRNSTNCKQFIQ 405

[Close]

BLAST of Peptide chain release factor 1 vs. Planmine SMEST
Match: SMESG000022027.1 (SMESG000022027.1)

HSP 1 Score: 732.635 bits (1890), Expect = 0.000e+0
Identity = 372/373 (99.73%), Postives = 373/373 (100.00%), Query Frame = 1

Query:  163 MPKYLKDELINFQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTXXXXXXXXXXXXXXEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKSLQEFDEFDQLELALRNSTNCKQFIQ 1281
            MPKYLKDELINFQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTVAVIVLPVLPDDVVEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIK+LQEFDEFDQLELALRNSTNCKQFIQ
Sbjct:    1 MPKYLKDELINFQNEKLNEFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSLLDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMDILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCKVNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTVAVIVLPVLPDDVVEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQQNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQDRITEHRIHKNFSNIQNILNQGYFDEIIKNLQEFDEFDQLELALRNSTNCKQFIQ 373

The following BLAST results are available for this feature:

BLAST of Peptide chain release factor 1 vs. Ensembl Human
BLAST of Peptide chain release factor 1 vs. Ensembl Celegans
BLAST of Peptide chain release factor 1 vs. Ensembl Fly
BLAST of Peptide chain release factor 1 vs. Ensembl Zebrafish
BLAST of Peptide chain release factor 1 vs. Ensembl Xenopus
BLAST of Peptide chain release factor 1 vs. Ensembl Mouse
BLAST of Peptide chain release factor 1 vs. UniProt/SwissProt
BLAST of Peptide chain release factor 1 vs. TrEMBL
BLAST of Peptide chain release factor 1 vs. Ensembl Cavefish
BLAST of Peptide chain release factor 1 vs. Ensembl Sea Lamprey
BLAST of Peptide chain release factor 1 vs. Ensembl Yeast
BLAST of Peptide chain release factor 1 vs. Ensembl Nematostella
BLAST of Peptide chain release factor 1 vs. Ensembl Medaka
BLAST of Peptide chain release factor 1 vs. Planmine SMEST

BLAST of Peptide chain release factor 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5


Match Name	E-value	Identity	Description
MTRF1L	2.578e-71	37.66	mitochondrial translational release factor 1 like ...	[more]
MTRF1L	6.231e-56	44.44	mitochondrial translational release factor 1 like ...	[more]
MTRF1	2.124e-54	34.95	mitochondrial translation release factor 1 [Source...	[more]
MTRF1	2.124e-54	34.95	mitochondrial translation release factor 1 [Source...	[more]
MTRF1L	4.057e-40	36.79	mitochondrial translational release factor 1 like ...	[more]

BLAST of Peptide chain release factor 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4


Match Name	E-value	Identity	Description
W03F8.3	4.996e-57	34.80	Probable peptide chain release factor 1, mitochond...	[more]
W03F8.3	1.514e-10	33.73	Probable peptide chain release factor 1, mitochond...	[more]
T23B5.4	7.912e-7	32.50	pep chromosome:WBcel235:IV:12675207:12676000:1 gen...	[more]
T23B5.4	2.045e-6	36.99	pep chromosome:WBcel235:IV:12675216:12675966:1 gen...	[more]

BLAST of Peptide chain release factor 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 3


Match Name	E-value	Identity	Description
mRF1	2.313e-61	34.79	gene:FBgn0032486 transcript:FBtr0080444	[more]
CG30100	2.318e-8	41.54	gene:FBgn0050100 transcript:FBtr0340022	[more]
CG30100	2.318e-8	41.54	gene:FBgn0050100 transcript:FBtr0087140	[more]

BLAST of Peptide chain release factor 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5


Match Name	E-value	Identity	Description
mtrf1l	1.320e-76	36.89	mitochondrial translational release factor 1-like ...	[more]
mtrf1	5.961e-36	39.89	mitochondrial translational release factor 1 [Sour...	[more]
mtrf1l	6.431e-26	29.18	mitochondrial translational release factor 1-like ...	[more]
si:ch211-275j6.5	4.030e-6	37.10	si:ch211-275j6.5 [Source:NCBI gene;Acc:100004874]	[more]
si:ch211-275j6.5	4.030e-6	37.10	si:ch211-275j6.5 [Source:NCBI gene;Acc:100004874]	[more]

BLAST of Peptide chain release factor 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2


Match Name	E-value	Identity	Description
commd1	1.447e-74	43.90	copper metabolism domain containing 1 [Source:Xenb...	[more]
etv1	1.434e-62	38.42	ETS variant transcription factor 1 [Source:Xenbase...	[more]

BLAST of Peptide chain release factor 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3


Match Name	E-value	Identity	Description
Mtrf1l	8.874e-69	40.23	mitochondrial translational release factor 1-like ...	[more]
Mtrf1	5.692e-57	34.62	mitochondrial translational release factor 1 [Sour...	[more]
2810006K23Rik	2.771e-6	38.33	RIKEN cDNA 2810006K23 gene [Source:MGI Symbol;Acc:...	[more]

BLAST of Peptide chain release factor 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5


Match Name	E-value	Identity	Description
sp\|Q3A928\|RF1_CARHZ	2.864e-72	39.09	Peptide chain release factor 1 OS=Carboxydothermus...	[more]
sp\|Q3A129\|RF1_PELCD	4.246e-71	39.81	Peptide chain release factor 1 OS=Pelobacter carbi...	[more]
sp\|Q9K6F4\|RF1_BACHD	5.856e-71	39.40	Peptide chain release factor 1 OS=Bacillus halodur...	[more]
sp\|Q4V7E5\|RF1ML_RAT	9.632e-71	40.98	Peptide chain release factor 1-like, mitochondrial...	[more]
sp\|Q9UGC7\|RF1ML_HUMAN	1.238e-70	37.66	Peptide chain release factor 1-like, mitochondrial...	[more]

BLAST of Peptide chain release factor 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
V9KT85	5.114e-79	42.24	Peptide chain release factor 1-like, mitochondrial...	[more]
A0A4W3I595	6.042e-79	42.24	RF_PROK_I domain-containing protein OS=Callorhinch...	[more]
A0A0G0IRH8	7.783e-76	41.97	Peptide chain release factor 1 OS=Parcubacteria gr...	[more]
A1ZI31	5.404e-75	40.17	Peptide chain release factor 1 OS=Microscilla mari...	[more]
K1ZC72	7.759e-75	46.23	PCRF domain-containing protein OS=uncultured bacte...	[more]

BLAST of Peptide chain release factor 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3


Match Name	E-value	Identity	Description
mtrf1l	4.899e-63	42.53	mitochondrial translational release factor 1-like ...	[more]
mtrf1l	2.173e-55	40.00	mitochondrial translational release factor 1-like ...	[more]
mtrf1	8.599e-47	41.63	mitochondrial translational release factor 1 [Sour...	[more]

BLAST of Peptide chain release factor 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3


Match Name	E-value	Identity	Description
mtrf1l	2.491e-64	38.44	mitochondrial translational release factor 1-like ...	[more]
mtrf1	1.598e-45	35.14	mitochondrial translational release factor 1 [Sour...	[more]
si:ch211-275j6.5	1.808e-8	32.53	chromosome 12 open reading frame 65 [Source:HGNC S...	[more]

BLAST of Peptide chain release factor 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 2


Match Name	E-value	Identity	Description
MRF1	1.459e-50	34.45	Mitochondrial translation release factor; involved...	[more]
YLR281C_mRNA	5.968e-8	50.00	Putative protein of unknown function; green fluore...	[more]

BLAST of Peptide chain release factor 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1


Match Name	E-value	Identity	Description
EDO42717	3.414e-65	42.34	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of Peptide chain release factor 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3


Match Name	E-value	Identity	Description
mtrf1l	6.711e-67	35.51	mitochondrial translational release factor 1 like ...	[more]
mtrf1	1.504e-62	32.63	mitochondrial translation release factor 1 [Source...	[more]
si:ch211-275j6.5	6.979e-6	38.33	chromosome 9 C12orf65 homolog [Source:NCBI gene;Ac...	[more]

BLAST of Peptide chain release factor 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2


Match Name	E-value	Identity	Description
SMESG000022027.1	0.000e+0	99.75	SMESG000022027.1	[more]
SMESG000022027.1	0.000e+0	99.73	SMESG000022027.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30002534 ID=SMED30002534|Name=Peptide chain release factor 1|organism=Schmidtea mediterranea sexual|type=transcript|length=1298bp
GTTGCTTAGAATTAGGGTTTCTGTCCTATTTGTTTTTCAATTTATATTAC
ATTTTAGCTTATAAAAATGAATTTCATTAATAAATTCCTTAAAACATCAC
AATTGTTCAATAATTTTCGTTTTATCGACTATAAAACTTTTACAAACATA
TCATATATCAATATGCCTAAGTATTTAAAGGACGAATTAATAAATTTTCA
AAATGAAAAACTAAACGAATTTGATCGAATCAATAGTCTTATTCAATCAC
AGAGTCTAAGCAACAGAGATTTATTGGAACAATATGGACGTTTATCTTCA
CTGGTAGATATATTTAATGACATCGAATTAGTAAATGTAGATGAGCAATA
TTTGAATAACTCATTATTAGATTCTGACACGGAACCGGAAATGGCTGATC
TAATGCGATTAGAAAGTAATCAACTGTCTGCACGACGGGATGCTTTATAC
AGCAAACTCATTTCCATGGTTATTCCACACAACGATTTGAACGAGGCTTC
CGAAATCTCGATGGACATATTATCCGGAGCTGGAGGCCAGGAATCAATGT
TGTTTGCTGAAGATTTGTTAAATGCATATAAAAAATTTGCAACTGCTAAA
AATTGGATTTTTGAAGAAGTTAATATTAATTATTGCGCTGAAATGGGTGG
AATACGCTCATGCAAAGTCAATTTCAGCGGTAACCAAGTGTACAACTGTT
TAAAATACGAATCCGGCGTCCACAGGGTACAAAGAGTACCAATTACCAGT
TCCCAGGGTAAAATACATACGAGTACAGTGGCAGTTATTGTGCTCCCTGT
TTTACCGGATGATGTTGTCGAAATAAAAGAATCCGATATAAAATTCGAAT
CATTCAAATCTAGTGGACCAGGTGGTCAACACGTTAATACAACAGATAGT
GCAGTGAGGTTAGTCCATATTCCAACGAAAATAGTAGTTTCGTGCCAGAA
AGAAAAAATACAGCAGCAAAATAAAAAAACTGCAATGCAACAGTTGAAAG
ATTTACTTATCGAGAAACAATATGAAGAAAAAATGAATAGATTGAATTCT
CATCGAAAGATCGTAATTGGAACTAGGTCTAGGTCAGATAAAATTCGAAC
TTATAATTACACCCAAGATCGAATTACTGAGCATAGAATTCATAAAAACT
TTTCCAATATTCAAAATATTCTTAATCAGGGATATTTTGATGAAATCATC
AAAAGTTTACAAGAATTTGATGAATTCGACCAATTGGAATTAGCGCTGAG
GAATTCAACTAATTGTAAACAATTTATCCAATAAATTTTGACCAGTCC

protein sequence of SMED30002534-orf-1

>SMED30002534-orf-1 ID=SMED30002534-orf-1|Name=SMED30002534-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=406bp
MNFINKFLKTSQLFNNFRFIDYKTFTNISYINMPKYLKDELINFQNEKLN
EFDRINSLIQSQSLSNRDLLEQYGRLSSLVDIFNDIELVNVDEQYLNNSL
LDSDTEPEMADLMRLESNQLSARRDALYSKLISMVIPHNDLNEASEISMD
ILSGAGGQESMLFAEDLLNAYKKFATAKNWIFEEVNINYCAEMGGIRSCK
VNFSGNQVYNCLKYESGVHRVQRVPITSSQGKIHTSTVAVIVLPVLPDDV
VEIKESDIKFESFKSSGPGGQHVNTTDSAVRLVHIPTKIVVSCQKEKIQQ
QNKKTAMQQLKDLLIEKQYEEKMNRLNSHRKIVIGTRSRSDKIRTYNYTQ
DRITEHRIHKNFSNIQNILNQGYFDEIIKSLQEFDEFDQLELALRNSTNC
KQFIQ*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0002109	X1 cell

Vocabulary: **INTERPRO**
Term	Definition
IPR005139	PCRF
IPR000352	Pep_chain_release_fac_I
IPR014720	dsRBD_dom

Vocabulary: **cellular component**
Term	Definition
GO:0005737	cytoplasm

Vocabulary: **biological process**
Term	Definition
GO:0006415	translational termination
GO:0006412	translation

Vocabulary: **molecular function**
Term	Definition
GO:0016149	translation release factor activity, codon specific
GO:0003747	translation release factor activity

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005139	Peptide chain release factor	SMART	SM00937	PCRF_a_2	coord: 99..215 e-value: 6.0E-19 score: 79.0
IPR005139	Peptide chain release factor	PFAM	PF03462	PCRF	coord: 56..240 e-value: 3.7E-44 score: 150.9
IPR000352	Peptide chain release factor class I	PFAM	PF00472	RF-1	coord: 251..357 e-value: 2.6E-33 score: 114.4
None	No IPR available	GENE3D	G3DSA:3.30.70.1660		coord: 149..377 e-value: 5.1E-79 score: 266.5
None	No IPR available	GENE3D	G3DSA:3.30.160.20		coord: 247..327 e-value: 5.1E-79 score: 266.5
None	No IPR available	PANTHER	PTHR43804	FAMILY NOT NAMED	coord: 66..390
None	No IPR available	PANTHER	PTHR43804:SF3	PEPTIDE CHAIN RELEASE FACTOR 1-LIKE, MITOCHONDRIAL	coord: 66..390
None	No IPR available	SUPERFAMILY	SSF75620	Release factor	coord: 65..390