achaete-scute homolog 3

Overview
Nameachaete-scute homolog 3
Smed IDSMED30002500
Length (bp)972
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of achaete-scute homolog 3 (SMED30002500) t-SNE clustered cells

Violin plots show distribution of expression levels for achaete-scute homolog 3 (SMED30002500) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of achaete-scute homolog 3 (SMED30002500) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for achaete-scute homolog 3 (SMED30002500) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002500

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30002500h1SMcG0016534 dd_Smed_v4_14753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30002500h1SMcG0016534 dd_Smed_v4_14753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30002500h1SMcG0016534 dd_Smed_v4_14753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30002500h1SMcG0016534 dd_Smed_v4_14753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30002500h1SMcG0016534 dd_Smed_v4_14753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
progenitor cellSMED30002500h1SMcG0016534 KF487091smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
stem cellSMED30002500h1SMcG0016534 KF487091smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30002500h1SMcG0016534 KF487091smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of achaete-scute homolog 3 vs. Ensembl Human
Match: ASCL3 (achaete-scute family bHLH transcription factor 3 [Source:HGNC Symbol;Acc:HGNC:740])

HSP 1 Score: 53.5286 bits (127), Expect = 5.238e-8
Identity = 41/77 (53.25%), Postives = 56/77 (72.73%), Query Frame = 1 
Query:  310 PFNYPVPFPYHFSNHYNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKIL 540
            PF++P+P+P +    Y+   +F R+RNERER+RV+ VNEGY  LR  LP    EKR+SKVE LR+AI+YIN L+ +L
Sbjct:   73 PFSFPMPYPNYRGCEYSYGPAFTRKRNERERQRVKCVNEGYAQLRHHLPEEYLEKRLSKVETLRAAIKYINYLQSLL 149
[Close]
BLAST of achaete-scute homolog 3 vs. Ensembl Human
Match: ASCL5 (achaete-scute family bHLH transcription factor 5 [Source:HGNC Symbol;Acc:HGNC:33169])

HSP 1 Score: 48.521 bits (114), Expect = 3.395e-6
Identity = 42/74 (56.76%), Postives = 53/74 (71.62%), Query Frame = 1 
Query:  325 VPFPYHFSNH-YNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILS 543
            VPFP  F  + Y    +FI++RNERER+RV+ VNEGY  LR  LP    EKR+SKVE LR+AIRYI  L+++LS
Sbjct:   67 VPFPGAFGVYEYPFEPAFIQKRNERERQRVKCVNEGYARLRGHLPGALAEKRLSKVETLRAAIRYIKYLQELLS 140
[Close]
BLAST of achaete-scute homolog 3 vs. Ensembl Human
Match: ASCL5 (achaete-scute family bHLH transcription factor 5 [Source:HGNC Symbol;Acc:HGNC:33169])

HSP 1 Score: 48.521 bits (114), Expect = 3.395e-6
Identity = 42/74 (56.76%), Postives = 53/74 (71.62%), Query Frame = 1 
Query:  325 VPFPYHFSNH-YNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILS 543
            VPFP  F  + Y    +FI++RNERER+RV+ VNEGY  LR  LP    EKR+SKVE LR+AIRYI  L+++LS
Sbjct:   67 VPFPGAFGVYEYPFEPAFIQKRNERERQRVKCVNEGYARLRGHLPGALAEKRLSKVETLRAAIRYIKYLQELLS 140
[Close]
BLAST of achaete-scute homolog 3 vs. Ensembl Celegans
Match: hlh-6 (Helix-loop-helix protein 6 [Source:UniProtKB/Swiss-Prot;Acc:Q10007])

HSP 1 Score: 47.7506 bits (112), Expect = 4.642e-6
Identity = 38/75 (50.67%), Postives = 49/75 (65.33%), Query Frame = 1 
Query:  325 VPFPYHFSNHYNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQ 549
            VP P    + Y    S + +RNERER RVR VN+GY+ LR+ LP+   EKRISKV+ LR AIRYI  L+ +L  +
Sbjct:  158 VPLPVELEDQYGPYSSSVWKRNERERCRVRNVNDGYERLRKHLPVHFDEKRISKVDTLRLAIRYIKHLDNLLRSE 232
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BLAST of achaete-scute homolog 3 vs. Ensembl Xenopus
Match: cuzd1.2 (CUB and zona pellucida-like domains 1, gene 2 [Source:Xenbase;Acc:XB-GENE-5998436])

HSP 1 Score: 50.8322 bits (120), Expect = 1.674e-6
Identity = 29/70 (41.43%), Postives = 41/70 (58.57%), Query Frame = 1 
Query:  427 GYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQEQDIDESF---LKHMNQDIGNEESKITCD 627
             ++TL+RC    N  +R+ KVEILR+AIRYI +L+ +L GQE    ESF   L+H + D      +  C 
Sbjct:  114 AFETLKRCTST-NPNQRLPKVEILRNAIRYIESLQSLLRGQE----ESFYPVLEHYSGDSDASSPRSNCS 178
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BLAST of achaete-scute homolog 3 vs. Ensembl Mouse
Match: Ascl5 (achaete-scute family bHLH transcription factor 5 [Source:MGI Symbol;Acc:MGI:2685043])

HSP 1 Score: 49.6766 bits (117), Expect = 9.913e-7
Identity = 42/74 (56.76%), Postives = 53/74 (71.62%), Query Frame = 1 
Query:  325 VPFPYHFSNH-YNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILS 543
            VPFP  F  + Y    +FI++RNERER+RV+ VNEGY  LR  LP    EKR+SKVE LR+AIRYI  L+++LS
Sbjct:   64 VPFPGAFGVYDYPFEPAFIQKRNERERQRVKCVNEGYARLRGHLPGALTEKRLSKVETLRAAIRYIKYLQELLS 137
[Close]
BLAST of achaete-scute homolog 3 vs. UniProt/SwissProt
Match: sp|Q9NQ33|ASCL3_HUMAN (Achaete-scute homolog 3 OS=Homo sapiens OX=9606 GN=ASCL3 PE=1 SV=2)

HSP 1 Score: 53.5286 bits (127), Expect = 2.386e-7
Identity = 41/77 (53.25%), Postives = 56/77 (72.73%), Query Frame = 1 
Query:  310 PFNYPVPFPYHFSNHYNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKIL 540
            PF++P+P+P +    Y+   +F R+RNERER+RV+ VNEGY  LR  LP    EKR+SKVE LR+AI+YIN L+ +L
Sbjct:   72 PFSFPMPYPNYRGCEYSYGPAFTRKRNERERQRVKCVNEGYAQLRHHLPEEYLEKRLSKVETLRAAIKYINYLQSLL 148
[Close]
BLAST of achaete-scute homolog 3 vs. TrEMBL
Match: A0A0A7M1Z3 (Achaete-scute class B transcription factor (Fragment) OS=Schmidtea polychroa OX=50054 GN=ASCb PE=2 SV=1)

HSP 1 Score: 291.582 bits (745), Expect = 4.002e-96
Identity = 167/176 (94.89%), Postives = 173/176 (98.30%), Query Frame = 1 
Query:  103 SELSPSPFINSDIHNTDTNLPYWVAYPEMSAIYNPLSAHSIPSGKTRKTAVNNQNIHLNGNSVSTNHKDPFNYPVPFPYHFSNHYNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQEQDIDESFLKHMNQDIGNEESKITCDQ 630
            SELSPSPFINSDIHNTDTNLPYWV YPEMS IYNPLSAHSIPSGKTRKTAVNNQNI+LNGNSVS+NHKDPFNYPVPFPYHFSNHYNCAQS IRRRNERERERVRYVNEGYDTLRRCLPLPNR+KRISKVEILRSAIRYINTLEKILSGQEQD+D++FLKHMNQDIGNEESKITCDQ
Sbjct:    1 SELSPSPFINSDIHNTDTNLPYWVTYPEMSTIYNPLSAHSIPSGKTRKTAVNNQNINLNGNSVSSNHKDPFNYPVPFPYHFSNHYNCAQSSIRRRNERERERVRYVNEGYDTLRRCLPLPNRDKRISKVEILRSAIRYINTLEKILSGQEQDVDDTFLKHMNQDIGNEESKITCDQ 176
[Close]
BLAST of achaete-scute homolog 3 vs. TrEMBL
Match: U5YR51 (Ascl-2 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=ascl-2 PE=2 SV=1)

HSP 1 Score: 247.669 bits (631), Expect = 1.783e-79
Identity = 137/137 (100.00%), Postives = 137/137 (100.00%), Query Frame = 1 
Query:  160 LPYWVAYPEMSAIYNPLSAHSIPSGKTRKTAVNNQNIHLNGNSVSTNHKDPFNYPVPFPYHFSNHYNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQEQDIDES 570
            LPYWVAYPEMSAIYNPLSAHSIPSGKTRKTAVNNQNIHLNGNSVSTNHKDPFNYPVPFPYHFSNHYNCAQSFIRRRNERERERVRYVNEGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQEQDIDES
Sbjct:    1 LPYWVAYPEMSAIYNPLSAHSIPSGKTRKTAVNNQNIHLNGNSVSTNHKDPFNYPVPFPYHFSNHYNCAQSFIRRRNERERERVRYVNEGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQEQDIDES 137
[Close]
BLAST of achaete-scute homolog 3 vs. TrEMBL
Match: A0A2Y9RFQ5 (achaete-scute homolog 3 OS=Trichechus manatus latirostris OX=127582 GN=LOC101345933 PE=4 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 1.031e-6
Identity = 46/94 (48.94%), Postives = 66/94 (70.21%), Query Frame = 1 
Query:  277 NGNSVSTNHKDP--FNYPVPFPYHFSNHYNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQE 552
            +G+ +  N+ DP  F++P+P+P +    Y+   +FIR+RNERER+RV+ VNEGY  LR  LP    EKR+SKVE LR+AI+YIN L+ +L  +E
Sbjct:   60 SGSMIMENYGDPCTFSFPMPYPNYRRCEYSYGPAFIRKRNERERQRVKCVNEGYARLRHHLPEEYLEKRLSKVETLRAAIKYINYLQSLLYPEE 153
[Close]
BLAST of achaete-scute homolog 3 vs. TrEMBL
Match: H0XAA9 (Achaete-scute family bHLH transcription factor 3 OS=Otolemur garnettii OX=30611 GN=ASCL3 PE=4 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 3.871e-6
Identity = 42/77 (54.55%), Postives = 57/77 (74.03%), Query Frame = 1 
Query:  310 PFNYPVPFPYHFSNHYNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKIL 540
            P ++P+P+P +    Y+C  +FIR+RNERER+RV+ VNEGY  LR  LP    EKR+SKVE LR+AI+YIN L+ +L
Sbjct:   73 PISFPMPYPNYRRCDYSCGPAFIRKRNERERQRVKCVNEGYAQLRHHLPEEYLEKRLSKVETLRAAIKYINYLQSLL 149
[Close]
BLAST of achaete-scute homolog 3 vs. TrEMBL
Match: W5Q6R4 (Achaete-scute family bHLH transcription factor 3 OS=Ovis aries OX=9940 GN=ASCL3 PE=4 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 8.817e-6
Identity = 46/96 (47.92%), Postives = 64/96 (66.67%), Query Frame = 1 
Query:  289 VSTNHKDP--FNYPVPFPYHFSNHYNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQEQDIDES 570
            +  N+ +P  F++PVP+P +    Y+   +FIR+RNERER+RV+ VNEGY  LR  LP    EKR+SKVE LR+AI+YIN L+ +L   E +   S
Sbjct:   64 IMENYGEPCAFSFPVPYPNYRRCEYSYGPAFIRKRNERERQRVKCVNEGYAQLRHHLPEEYLEKRLSKVETLRAAIKYINYLQSLLYPDEAETKNS 159
[Close]
BLAST of achaete-scute homolog 3 vs. Ensembl Nematostella
Match: NVAS-C2 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1A5])

HSP 1 Score: 45.0542 bits (105), Expect = 1.072e-6
Identity = 39/65 (60.00%), Postives = 47/65 (72.31%), Query Frame = 1 
Query:  355 YNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQ 549
            YN   +FIR+RNERER RVR+VNEGY  LR  LP    +KR+SKVE LR+AIRYI  LE +L  +
Sbjct:    4 YNLEPAFIRKRNERERIRVRHVNEGYARLREHLPEEPSDKRMSKVETLRAAIRYIKHLESLLQAE 68
[Close]
BLAST of achaete-scute homolog 3 vs. Ensembl Nematostella
Match: NVATO2 (Atonal-related protein 2; Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJ22])

HSP 1 Score: 47.7506 bits (112), Expect = 1.708e-6
Identity = 20/54 (37.04%), Postives = 32/54 (59.26%), Query Frame = 1 
Query:  433 DTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQEQDIDESFLKHMNQD 594
            D+L++C+P+P  ++R++K+EILR A  YI +L   L G+       F K    D
Sbjct:  162 DSLKKCIPVPQSKRRVTKLEILRIACNYIKSLSDTLRGESSGPSSVFRKRARAD 215
[Close]
BLAST of achaete-scute homolog 3 vs. Planmine SMEST
Match: SMESG000032619.1 (SMESG000032619.1)

HSP 1 Score: 353.599 bits (906), Expect = 6.388e-124
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 1 
Query:   67 MQRYRNNSYNFLSELSPSPFINSDIHNTDTNLPYWVAYPEMSAIYNPLSAHSIPSGKTRKTAVNNQNIHLNGNSVSTNHKDPFNYPVPFPYHFSNHYNCAQSFIXXXXXXXXXXXXXXXXGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQEQDIDESFLKHMNQDIGNEESKITCDQS 633
            MQRYRNNSYNFLSELSPSPFINSDIHNTDTNLPYWVAYPEMSAIYNPLSAHSIPSGKTRKTAVNNQNIHLNGNSVSTNHKDPFNYPVPFPYHFSNHYNCAQSFIRRRNERERERVRYVNEGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQEQDIDESFLKHMNQDIGNEESKITCDQS
Sbjct:    1 MQRYRNNSYNFLSELSPSPFINSDIHNTDTNLPYWVAYPEMSAIYNPLSAHSIPSGKTRKTAVNNQNIHLNGNSVSTNHKDPFNYPVPFPYHFSNHYNCAQSFIRRRNERERERVRYVNEGYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKILSGQEQDIDESFLKHMNQDIGNEESKITCDQS 189
[Close]
BLAST of achaete-scute homolog 3 vs. Planmine SMEST
Match: SMESG000026861.1 (SMESG000026861.1)

HSP 1 Score: 46.2098 bits (108), Expect = 3.664e-6
Identity = 19/38 (50.00%), Postives = 27/38 (71.05%), Query Frame = 1 
Query:  427 GYDTLRRCLPLPNREKRISKVEILRSAIRYINTLEKIL 540
             +  LR+C+P+ NR KR+SKV  LR AI YI +L ++L
Sbjct:   88 AFAELRKCIPIENRRKRVSKVRTLRVAIDYIKSLSQLL 125
The following BLAST results are available for this feature:
BLAST of achaete-scute homolog 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ASCL35.238e-853.25achaete-scute family bHLH transcription factor 3 [... [more]
ASCL53.395e-656.76achaete-scute family bHLH transcription factor 5 [... [more]
ASCL53.395e-656.76achaete-scute family bHLH transcription factor 5 [... [more]
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BLAST of achaete-scute homolog 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
hlh-64.642e-650.67Helix-loop-helix protein 6 [Source:UniProtKB/Swis... [more]
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BLAST of achaete-scute homolog 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of achaete-scute homolog 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of achaete-scute homolog 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
cuzd1.21.674e-641.43CUB and zona pellucida-like domains 1, gene 2 [Sou... [more]
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BLAST of achaete-scute homolog 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Ascl59.913e-756.76achaete-scute family bHLH transcription factor 5 [... [more]
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BLAST of achaete-scute homolog 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q9NQ33|ASCL3_HUMAN2.386e-753.25Achaete-scute homolog 3 OS=Homo sapiens OX=9606 GN... [more]
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BLAST of achaete-scute homolog 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0A7M1Z34.002e-9694.89Achaete-scute class B transcription factor (Fragme... [more]
U5YR511.783e-79100.00Ascl-2 protein (Fragment) OS=Schmidtea mediterrane... [more]
A0A2Y9RFQ51.031e-648.94achaete-scute homolog 3 OS=Trichechus manatus lati... [more]
H0XAA93.871e-654.55Achaete-scute family bHLH transcription factor 3 O... [more]
W5Q6R48.817e-647.92Achaete-scute family bHLH transcription factor 3 O... [more]
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BLAST of achaete-scute homolog 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of achaete-scute homolog 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of achaete-scute homolog 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of achaete-scute homolog 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
NVAS-C21.072e-660.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
NVATO21.708e-637.04Atonal-related protein 2; Predicted protein [Sour... [more]
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BLAST of achaete-scute homolog 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of achaete-scute homolog 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000032619.16.388e-124100.00SMESG000032619.1[more]
SMESG000026861.13.664e-650.00SMESG000026861.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002500 ID=SMED30002500|Name=achaete-scute homolog 3|organism=Schmidtea mediterranea sexual|type=transcript|length=972bp
AACTAAACTATCAAATGGTAGATAATTGTTGTATAATTAATTTTATTAAT
TTCAATTTCAAGAAAAATGCAGCGGTATAGAAATAACTCGTACAATTTTC
TCTCAGAACTTTCTCCTTCACCATTTATAAACAGTGATATTCATAATACA
GATACAAATCTCCCATATTGGGTTGCTTATCCAGAAATGTCAGCAATTTA
TAATCCATTATCTGCTCATTCTATTCCATCTGGGAAAACCCGGAAAACGG
CAGTTAACAATCAAAATATACATCTAAACGGAAATTCAGTTTCTACAAAT
CATAAAGATCCTTTCAATTACCCAGTTCCCTTTCCTTATCACTTTTCCAA
TCATTATAATTGTGCTCAGAGCTTCATAAGGAGACGCAATGAAAGAGAAC
GTGAACGAGTTCGTTATGTCAATGAAGGCTACGATACACTGCGCAGATGC
TTACCGTTGCCGAATCGAGAAAAACGGATAAGTAAAGTAGAGATTTTACG
ATCAGCCATTCGATACATTAACACGTTGGAAAAAATTCTGTCAGGTCAAG
AACAAGATATCGACGAGTCTTTTCTTAAACATATGAACCAAGATATTGGT
AATGAAGAATCGAAAATAACTTGCGATCAATCAATAATTATAGATTTAAA
CAAATAAAAATATTAAATTTTAAAAAAAAATTTTGAAAATAAAAATGATC
AGAAAATAAAATTTGCTATGCATTGCCAACTGTTTCGTTGATATTTATAT
TTTACATAAAAATGTTTTGTCAAAATATTTCAACTTTCAAATTCATATAC
AGATACAACATAATTCTGTAAAACTGTTTGATACCCCCATTTTAGTAAAT
GCCAATGACCTTAAACTTACAAAGGAAAATAAACCTAAGCCTAGCTGCAA
CTTATACTTCAGTATAAAATCGTCAAGTCTATGACTTTTTATTTGTTTTA
TATTGTTTTATCGTTGTGAAGA
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protein sequence of SMED30002500-orf-1

>SMED30002500-orf-1 ID=SMED30002500-orf-1|Name=SMED30002500-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=197bp
MQRYRNNSYNFLSELSPSPFINSDIHNTDTNLPYWVAYPEMSAIYNPLSA
HSIPSGKTRKTAVNNQNIHLNGNSVSTNHKDPFNYPVPFPYHFSNHYNCA
QSFIRRRNERERERVRYVNEGYDTLRRCLPLPNREKRISKVEILRSAIRY
INTLEKILSGQEQDIDESFLKHMNQDIGNEESKITCDQSIIIDLNK*
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Aliases
The feature 'achaete-scute homolog 3' has the following synonyms
Synonym
ascl-2
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0006357regulation of transcription from RNA polymerase II promoter
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000120progenitor cell
PLANA:0000121stem cell
PLANA:0002142parenchyma
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR036638HLH_DNA-bd_sf
IPR015660MASH1/Ascl1a-like
IPR011598bHLH_dom
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
GO:0003677DNA binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 108..160
e-value: 2.6E-13
score: 60.2
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 106..155
e-value: 2.9E-14
score: 52.7
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 102..154
score: 14.107
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainCDDcd00083HLHcoord: 118..159
e-value: 2.32804E-10
score: 52.2163
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3DG3DSA:4.10.280.10coord: 105..160
e-value: 2.0E-18
score: 67.9
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILYSSF47459HLH, helix-loop-helix DNA-binding domaincoord: 105..169
NoneNo IPR availablePANTHERPTHR13935:SF68ACHAETE-SCUTE HOMOLOG 3coord: 88..165
IPR015660Achaete-scute transcription factor-relatedPANTHERPTHR13935ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATEDcoord: 88..165