NPHS1-5
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30002472 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of NPHS1-5 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 71.2478 bits (173), Expect = 1.104e-11 Identity = 62/247 (25.10%), Postives = 105/247 (42.51%), Query Frame = 2
Query: 245 IVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHP-LLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQ-DKVTVNDRDSLRVSCSLSPPGLPKALLEW 979
+ +V +RC V VQW + +G D IP PR+R+ GD +R E+ L I SD+ +YECQV + P L+ R L + P ++ + M + Q+ ++ C+S P P + ++ + + + N NE + + + P ++ L+ C S+ AL P V + F P + + P D+ V SL + C ++ G P A L+W
Sbjct: 44 VEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPK---LLLLTPEAGTMVTWVAGQEYVVN--CVSGDAKPAPDITILLSGQTISDISA--NVNEGSQQKLFTV-EATARVTPRSSDNRQLLVCEASSPAL---EAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPC-VARGGNPLATLQW 278 HSP 2 Score: 63.1586 bits (152), Expect = 4.034e-9 Identity = 58/241 (24.07%), Postives = 105/241 (43.57%), Query Frame = 2
Query: 2687 KCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQ--PEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADI--HWFKDG-----NDSRILYSTNITSNSSDVIETAIFFANISDAQ-FGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGES 3379
K S+ + L ++ G Y C VD + + +L ++++ P++ + K S S + G +I W K+G D R T T + V + + AN+S AQ + ++C +N G D +++V + PD P+ V S+ + W+ GFDGG Q F I++ + +H + P Q+ ++ + L P++ Y + + ASN G+S
Sbjct: 792 KISRGPTGRLRIHHAKLAQAGAYQC--IVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRY---TEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRY--EALGTPGFHYVDVVPP---QATTFTLTGLQPSTRYRVWLLASNALGDS 1022
BLAST of NPHS1-5 vs. Ensembl Human
Match: NPHS1 (NPHS1 adhesion molecule, nephrin [Source:HGNC Symbol;Acc:HGNC:7908]) HSP 1 Score: 71.2478 bits (173), Expect = 1.134e-11 Identity = 62/247 (25.10%), Postives = 105/247 (42.51%), Query Frame = 2
Query: 245 IVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHP-LLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQ-DKVTVNDRDSLRVSCSLSPPGLPKALLEW 979
+ +V +RC V VQW + +G D IP PR+R+ GD +R E+ L I SD+ +YECQV + P L+ R L + P ++ + M + Q+ ++ C+S P P + ++ + + + N NE + + + P ++ L+ C S+ AL P V + F P + + P D+ V SL + C ++ G P A L+W
Sbjct: 44 VEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPK---LLLLTPEAGTMVTWVAGQEYVVN--CVSGDAKPAPDITILLSGQTISDISA--NVNEGSQQKLFTV-EATARVTPRSSDNRQLLVCEASSPAL---EAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPC-VARGGNPLATLQW 278 HSP 2 Score: 63.1586 bits (152), Expect = 3.835e-9 Identity = 58/241 (24.07%), Postives = 105/241 (43.57%), Query Frame = 2
Query: 2687 KCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQ--PEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADI--HWFKDG-----NDSRILYSTNITSNSSDVIETAIFFANISDAQ-FGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGES 3379
K S+ + L ++ G Y C VD + + +L ++++ P++ + K S S + G +I W K+G D R T T + V + + AN+S AQ + ++C +N G D +++V + PD P+ V S+ + W+ GFDGG Q F I++ + +H + P Q+ ++ + L P++ Y + + ASN G+S
Sbjct: 792 KISRGPTGRLRIHHAKLAQAGAYQC--IVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRY---TEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRY--EALGTPGFHYVDVVPP---QATTFTLTGLQPSTRYRVWLLASNALGDS 1022
BLAST of NPHS1-5 vs. Ensembl Human
Match: KIRREL1 (kirre like nephrin family adhesion molecule 1 [Source:HGNC Symbol;Acc:HGNC:15734]) HSP 1 Score: 67.3958 bits (163), Expect = 1.463e-10 Identity = 54/220 (24.55%), Postives = 83/220 (37.73%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRP 871
F + P D+ + V+ C + N G VQW + +G + + A PR+R+ G A +Y L I + SD+ YECQ L RR L V P + +++ Q +L C + + P T W D E S E D ++ S NP+ L + C N A+ PS T +++ P
Sbjct: 23 FSQEPADQ-TVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQA---TEAALRSRRAKLTVLIPP-------EDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVA---STELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAI-----PSGKETSIELDVHHPP 223
BLAST of NPHS1-5 vs. Ensembl Human
Match: KIRREL2 (kirre like nephrin family adhesion molecule 2 [Source:HGNC Symbol;Acc:HGNC:18816]) HSP 1 Score: 57.7658 bits (138), Expect = 1.465e-8 Identity = 40/155 (25.81%), Postives = 65/155 (41.94%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKH-GFPIPTYSWIVDNKFLES 673
F + P+D ++ + + CA+ G VQW + +G R +P R+ I+G+A+ ++ L I P++ D YECQ L R L V P P ++ +VS + +L C S+ P P W D L+
Sbjct: 26 FLQQPEDLV-VLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQA---TQAGLRSRPAQLHVLVPPEAPQVL-GGPSVSLV------AGVPANLTCRSRGDARPTPELLWFRDGVLLDG 169
BLAST of NPHS1-5 vs. Ensembl Human
Match: KIRREL2 (kirre like nephrin family adhesion molecule 2 [Source:HGNC Symbol;Acc:HGNC:18816]) HSP 1 Score: 57.3806 bits (137), Expect = 1.628e-7 Identity = 51/221 (23.08%), Postives = 85/221 (38.46%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKH-GFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRP 871
F + P+D ++ + + CA+ G VQW + +G R +P R+ I+G+A+ ++ L I P++ D YECQ L R L V P P ++ +VS + + +L C S+ P P W D L+ + + S + S P D C ++AL P+ T + + Q P
Sbjct: 26 FLQQPEDLV-VLLGEEARLPCALGAYWGLVQWTKSGLALGGQRDLPGWSRYWISGNAANGQHDLHIRPVELEDEASYECQA---TQAGLRSRPAQLHVLVPPEAPQVL-GGPSVSLVAGVP------ANLTCRSRGDARPTPELLWFRDGVLLDGATFHQTLLKEGTPGSVE---STLTLTPFSHDDGATFVCRARSQAL-----PTGRDTAITLSLQYPP 227
BLAST of NPHS1-5 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 87.4261 bits (215), Expect = 4.946e-17 Identity = 126/562 (22.42%), Postives = 227/562 (40.39%), Query Frame = 2
Query: 1592 GDEIQVNCTVGDGNPIPDLAMY-----IDGIQIVKSKFSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTK-QFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFV--IDVIANPEEDLIC--VNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKD-----GNDSRILYSTNITSNSSDVIETAIFFANI-SDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQW 3229
GD + V C GNP+ +L+ Y ++G + FS + + + ++R + R+ C+A + +++S+Y LS+ PR V+++ + + G+ + C SNP ISW+ K ++ + +D + F+ N VL + P+ Y + V C + N+ + + S +NV++ PK+ N + +V EG +F + V NP L N I R+ F LS G + A+N++G T+ I++ + T ++ + T ++ C + N+ W + E + +RG + L + +++ S + Y + DNG V LLV S +L + K I G A + C V + T+ + W KD N S+ YS T E+ ++ NI D K C N G D ++ ++ PT PD+P + T+ + ++ W+ FDGG QIF++++
Sbjct: 447 GDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVAGFSSYS-TLALRVDRTMNTQRLKCEATNAALDEPLIESQY--LSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHLGDVSLNETTRD-----NGFNVEN--------VLSFTPTEEYDGTVVHC-IANHPEWKHSVNTSFPLNVMYPPKMLVN---DPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRV--FARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIH-W------YKNGEILAAQHRG-------HKKAILRLNATEQQSGE----YTCKADNGIGVPAEGQTFLLVNSAPRVLPVA---RYAKTAGI--LGGVAKARCKVYAVPTV----EFVWEKDEQLIKKNSSK--YSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKY 957
BLAST of NPHS1-5 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 87.0409 bits (214), Expect = 6.043e-17 Identity = 126/562 (22.42%), Postives = 227/562 (40.39%), Query Frame = 2
Query: 1592 GDEIQVNCTVGDGNPIPDLAMY-----IDGIQIVKSKFSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTK-QFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFV--IDVIANPEEDLIC--VNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKD-----GNDSRILYSTNITSNSSDVIETAIFFANI-SDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQW 3229
GD + V C GNP+ +L+ Y ++G + FS + + + ++R + R+ C+A + +++S+Y LS+ PR V+++ + + G+ + C SNP ISW+ K ++ + +D + F+ N VL + P+ Y + V C + N+ + + S +NV++ PK+ N + +V EG +F + V NP L N I R+ F LS G + A+N++G T+ I++ + T ++ + T ++ C + N+ W + E + +RG + L + +++ S + Y + DNG V LLV S +L + K I G A + C V + T+ + W KD N S+ YS T E+ ++ NI D K C N G D ++ ++ PT PD+P + T+ + ++ W+ FDGG QIF++++
Sbjct: 145 GDIVNVTCEAQGGNPLAELSWYRGSEKVNGAHNEVAGFSSYS-TLALRVDRTMNTQRLKCEATNAALDEPLIESQY--LSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHLGDVSLNETTRD-----NGFNVEN--------VLSFTPTEEYDGTVVHC-IANHPEWKHSVNTSFPLNVMYPPKMLVN---DPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRV--FARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIH-W------YKNGEILAAQHRG-------HKKAILRLNATEQQSGE----YTCKADNGIGVPAEGQTFLLVNSAPRVLPVA---RYAKTAGI--LGGVAKARCKVYAVPTV----EFVWEKDEQLIKKNSSK--YSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKY 655
BLAST of NPHS1-5 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 83.5741 bits (205), Expect = 8.149e-16 Identity = 128/574 (22.30%), Postives = 229/574 (39.90%), Query Frame = 2
Query: 1592 GDEIQVNCTVGDGNPIPDLAMYIDGIQ-IVKSKFSMR---------------AISTRII-IERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTK-QFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFV--IDVIANPEEDLIC--VNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKD-----GNDSRILYSTNITSNSSDVIETAIFFANI-SDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQW 3229
GD + V C GNP+ +L+ Y + +VK + M+ + ST + ++R + R+ C+A + +++S+Y LS+ PR V+++ + + G+ + C SNP ISW+ K ++ + +D + F+ N VL + P+ Y + V C + N+ + + S +NV++ PK+ N + +V EG +F + V NP L N I R+ F LS G + A+N++G T+ I++ + T ++ + T ++ C + N+ W + E + +RG + L + +++ S + Y + DNG V LLV S +L + K I G A + C V + T+ + W KD N S+ YS T E+ ++ NI D K C N G D ++ ++ PT PD+P + T+ + ++ W+ FDGG QIF++++
Sbjct: 447 GDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQY--LSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHLGDVSLNETTRD-----NGFNVEN--------VLSFTPTEEYDGTVVHC-IANHPEWKHSVNTSFPLNVMYPPKMLVN---DPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRV--FARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIH-W------YKNGEILAAQHRG-------HKKAILRLNATEQQSGE----YTCKADNGIGVPAEGQTFLLVNSAPRVLPVA---RYAKTAGI--LGGVAKARCKVYAVPTV----EFVWEKDEQLIKKNSSK--YSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKY 970
BLAST of NPHS1-5 vs. Ensembl Celegans
Match: syg-2 (Synaptogenesis protein syg-2 [Source:UniProtKB/Swiss-Prot;Acc:Q9U3P2]) HSP 1 Score: 83.1889 bits (204), Expect = 9.374e-16 Identity = 128/574 (22.30%), Postives = 229/574 (39.90%), Query Frame = 2
Query: 1592 GDEIQVNCTVGDGNPIPDLAMYIDGIQ-IVKSKFSMR---------------AISTRII-IERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTK-QFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFV--IDVIANPEEDLIC--VNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKD-----GNDSRILYSTNITSNSSDVIETAIFFANI-SDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQW 3229
GD + V C GNP+ +L+ Y + +VK + M+ + ST + ++R + R+ C+A + +++S+Y LS+ PR V+++ + + G+ + C SNP ISW+ K ++ + +D + F+ N VL + P+ Y + V C + N+ + + S +NV++ PK+ N + +V EG +F + V NP L N I R+ F LS G + A+N++G T+ I++ + T ++ + T ++ C + N+ W + E + +RG + L + +++ S + Y + DNG V LLV S +L + K I G A + C V + T+ + W KD N S+ YS T E+ ++ NI D K C N G D ++ ++ PT PD+P + T+ + ++ W+ FDGG QIF++++
Sbjct: 145 GDIVNVTCEAQGGNPLAELSWYRGSEKHLVKGRKKMKWSQVNGAHNEVAGFSSYSTLALRVDRTMNTQRLKCEATNAALDEPLIESQY--LSVYYPPRRVLIRPADSGDQRLLVGKSARLVCGTLSSNPAAHISWQFSRATDDNKVHLGDVSLNETTRD-----NGFNVEN--------VLSFTPTEEYDGTVVHC-IANHPEWKHSVNTSFPLNVMYPPKMLVN---DPVTVVMSEGDSFKENLTVRGNPAISLWQWRKNGVPFDHTIGRV--FARGAVLSGKQLLSTDAGVYTLTATNSVGSTNITIKLAVEYSARITSISTPVIAATGDTVLLECEADGEPSKANMIH-W------YKNGEILAAQHRG-------HKKAILRLNATEQQSGE----YTCKADNGIGVPAEGQTFLLVNSAPRVLPVA---RYAKTAGI--LGGVAKARCKVYAVPTV----EFVWEKDEQLIKKNSSK--YSIVNTQIDYSTYESTLWIKNIVPDDYTKKVKCIARNSFGTDHLSIPIIPPTHPDEPFNLTMTNSTLNTISVAWEPRFDGGSDQIFEVKY 668
BLAST of NPHS1-5 vs. Ensembl Celegans
Match: syg-1 (Synaptogenesis protein syg-1 [Source:UniProtKB/Swiss-Prot;Acc:B1Q236]) HSP 1 Score: 78.1814 bits (191), Expect = 2.868e-14 Identity = 70/304 (23.03%), Postives = 120/304 (39.47%), Query Frame = 2
Query: 218 ETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRA--IPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKH-HPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVD----------------NKFL------------------ESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRA----LALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKR 1006
E P+D + V +T ++ C V++Q G VQW + +G DR +P R+R+ G A+ EY L I + D+ D+ CQ+ H ++ + L V +P+ P IV + ++ + I C+S+ G P PT W + +KF E+ ++ N SR D S S S+ F P D + CI + + +DS S R QI +P + + S ++C+++ L + W N+ ++
Sbjct: 27 EAPKDTLAA-VGETAILTCRVEHQQGPVQWMKDDFGLGTDRDKPLPGNKRYRMVGSAANGEYNLEISNVTLFDDDDFACQISESDHAKAVVSSKAKLTVLVRPTPPKIVKSHHSLKAI------AGDPITQSCLSRKGKPPPTIGWAIASDEHGKHIVSWLGESRSKFGGIHAKPEISQETVIAHVNETTQVEEGGNNSREDSSIYSIMSNLSFIPRPEDDHKYLICISQHMTFPNKIEVDSVKLSLRYAPQINLTVASKLP--------LRENGSALLACNVNAKPLDNVKISWYKGNQKLRE 315
BLAST of NPHS1-5 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0339277) HSP 1 Score: 130.568 bits (327), Expect = 4.025e-30 Identity = 233/1080 (21.57%), Postives = 401/1080 (37.13%), Query Frame = 2
Query: 104 QWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKV--TVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISW-----EFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICH---VGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQI---VKSKFSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQV-----DIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFI-----ETILGCNRGLYFNNATSRYLK-------LFVKCSKEDSTDLVFYYISQG---DNGRYVCNVTVDGLLVASTKYILSIL-MQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRI--LYSTNITSNSSDVI--ETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQW 3229
Q W I L RL VIV +S P ++ Q FR TP D + + +MRC V N G VQW +GF IP PR+ + GD + Y L I +D+ DY+CQV P + + +R L V P+ I S N S +++ +Q + L+CI + P W N + +D ES + + S K P D C ++AL+ D R VQ+ PG P I T+ ++ + C S G P A L W + I+ +AY + S + +E N+ + A S ++Q+ V + + F P + + GP A + G+I C T+ I W + + + I+ + W T TS++ V S ++ICH + + V ++ + VLY P IS + + G ++ C GNP+ L Y + +I +++ + I+ +Q + C+A + + +S LS+ P V ++ I P+ + G TI C + SNP +SW + I + + S F +T + Q +Y C+ N Q+ ++ ++V++RPK F V+GE + ANP P KW + D +T + G I A G + F ++ I +C + Q ++++ + V + +++ + N + + +L C + T ++K + VK S + + +I D G + C V + + + + IL I+ PEI K ++ + + C S W +D D I Y + D + E+ + ++ A +G Y C NE G+ + TV + + PD P + ++ T+ W GFDGG +++++
Sbjct: 43 QHW-IKHLQLLRL--LAVIVLASAPVPSHAQQ--QKFRTTPHD-LQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPASIEIKGYSHN-SKVEVRENQD---LQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTT--SSLKLKPGPDDDYTEYTCQARHKALSPD---MPMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCR-SRGGNPPAQLIWYKNGSQIR---MAY----RTSGRLSE-----NIYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVM---GPTEA------------RVGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGW-------TTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYME--GQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINSVIRAADKSVSAEITILANVSDNQAQYRCEASNSATEIPLFQS--TTLSVHFAPETVKIR-IEPEE--LRPGMEATIICDSSSSNPPAKLSWWKDGI-----PIEGINNTSKPGLWGGTVSTLEF----RVNVTQEMNGQ---VY-----TCQSANEAL-QRSAHEAVSLDVLYRPK-FVPPPSSTAVGVEGESLQVSLQTRANP-------------------TPVTYKW--TKDGTTIPQDGDHRIFAD---GGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVV----------------VEYGTTIKSVSENIVVNPGEDAMLSCT--VEGKPLTEEHVKWERVGYDMTVKTSTTFANGTSYLHIKDAKREDVGNFRC-VADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRY 1003
BLAST of NPHS1-5 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0088635) HSP 1 Score: 129.413 bits (324), Expect = 1.045e-29 Identity = 233/1081 (21.55%), Postives = 403/1081 (37.28%), Query Frame = 2
Query: 104 QWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKV--TVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISW-----EFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICH---VGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQI---VKSKFSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVIS-WRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQV-----DIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFI-----ETILGCNRGLYFNNATSRYLK-------LFVKCSKEDSTDLVFYYISQG---DNGRYVCNVTVDGLLVASTKYILSIL-MQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRI--LYSTNITSNSSDVI--ETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQW 3229
Q W I L RL VIV +S P ++ Q FR TP D + + +MRC V N G VQW +GF IP PR+ + GD + Y L I +D+ DY+CQV P + + +R L V P+ I S N S +++ +Q + L+CI + P W N + +D ES + + S K P D C ++AL+ D R VQ+ PG P I T+ ++ + C S G P A L W + I+ +AY + S + +EN + + A S ++Q+ V + + F P + + GP A + G+I C T+ I W + + + I+ + W T TS++ V S ++ICH + + V ++ + VLY P IS + + G ++ C GNP+ L Y + +I +++ + I+ +Q + C+A + + +S LS+ P V ++ I P+ + G TI C + SNP +S W+ + + I + + S F +T + Q +Y C+ N Q+ ++ ++V++RPK F V+GE + ANP P KW + D +T + G I A G + F ++ I +C + Q ++++ + V + +++ + N + + +L C + T ++K + VK S + + +I D G + C V + + + + IL I+ PEI K ++ + + C S W +D D I Y + D + E+ + ++ A +G Y C NE G+ + TV + + PD P + ++ T+ W GFDGG +++++
Sbjct: 43 QHW-IKHLQLLRL--LAVIVLASAPVPSHAQQ--QKFRTTPHD-LQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPASIEIKGYSHN-SKVEVRENQD---LQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTT--SSLKLKPGPDDDYTEYTCQARHKALSPDM---PMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCR-SRGGNPPAQLIWYKNGSQIR---MAY----RTSGRLSEN-----IYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVM---GPTEA------------RVGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGW-------TTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYME--GQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINSVIRAADKSVSAEITILANVSDNQAQYRCEASNSATEIPLFQS--TTLSVHFAPETVKIR-IEPEE--LRPGMEATIICDSSSSNPPAKLSWWKDGI------PIEGINNTSKPGLWGGTVSTLEF----RVNVTQEMNGQ---VY-----TCQSANEAL-QRSAHEAVSLDVLYRPK-FVPPPSSTAVGVEGESLQVSLQTRANP-------------------TPVTYKW--TKDGTTIPQDGDHRIFAD---GGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVV----------------VEYGTTIKSVSENIVVNPGEDAMLSCT--VEGKPLTEEHVKWERVGYDMTVKTSTTFANGTSYLHIKDAKREDVGNFRC-VADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRY 1003
BLAST of NPHS1-5 vs. Ensembl Fly
Match: sns (gene:FBgn0024189 transcript:FBtr0111043) HSP 1 Score: 128.642 bits (322), Expect = 1.597e-29 Identity = 233/1081 (21.55%), Postives = 403/1081 (37.28%), Query Frame = 2
Query: 104 QWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKV--TVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISW-----EFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICH---VGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQI---VKSKFSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVIS-WRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQV-----DIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFI-----ETILGCNRGLYFNNATSRYLK-------LFVKCSKEDSTDLVFYYISQG---DNGRYVCNVTVDGLLVASTKYILSIL-MQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRI--LYSTNITSNSSDVI--ETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQW 3229
Q W I L RL VIV +S P ++ Q FR TP D + + +MRC V N G VQW +GF IP PR+ + GD + Y L I +D+ DY+CQV P + + +R L V P+ I S N S +++ +Q + L+CI + P W N + +D ES + + S K P D C ++AL+ D R VQ+ PG P I T+ ++ + C S G P A L W + I+ +AY + S + +EN + + A S ++Q+ V + + F P + + GP A + G+I C T+ I W + + + I+ + W T TS++ V S ++ICH + + V ++ + VLY P IS + + G ++ C GNP+ L Y + +I +++ + I+ +Q + C+A + + +S LS+ P V ++ I P+ + G TI C + SNP +S W+ + + I + + S F +T + Q +Y C+ N Q+ ++ ++V++RPK F V+GE + ANP P KW + D +T + G I A G + F ++ I +C + Q ++++ + V + +++ + N + + +L C + T ++K + VK S + + +I D G + C V + + + + IL I+ PEI K ++ + + C S W +D D I Y + D + E+ + ++ A +G Y C NE G+ + TV + + PD P + ++ T+ W GFDGG +++++
Sbjct: 43 QHW-IKHLQLLRL--LAVIVLASAPVPSHAQQ--QKFRTTPHD-LQVLEGAEAMMRCEVANVAGAVQWTKDGFALGFSAVIPGFPRYSVLGDRKQGIYNLRISNASINDDADYQCQVGPARLNSAIRANAKLTVISPPASIEIKGYSHN-SKVEVRENQD---LQLKCIVANAKPAAQIVWYRGNVEYKPEKREDTVEESTAKRFTTT--SSLKLKPGPDDDYTEYTCQARHKALSPDM---PMRATVQLSVLYPPGPPYIEGYSAGETLRRGQTVELMCR-SRGGNPPAQLIWYKNGSQIR---MAY----RTSGRLSEN-----IYTFTAEAGDNKARFRCEASNVMSQNPLKAEVELSVLFAPTHVTVM---GPTEA------------RVGDIVPLTCTTAPSNPPAEIKWMVGGRQVRNATSKTIVSPEGGW-------TTTSNITAVVEPNKRSLVVICHGLNMQLTENVVSTHTINVLYPPAPPLISGYME--GQIIPAGSVQKLLCVSSGGNPLATLTWYKNDKRINSVIRAADKSVSAEITILANVSDNQAQYRCEASNSATEIPLFQS--TTLSVHFAPETVKIR-IEPEE--LRPGMEATIICDSSSSNPPAKLSWWKDGI------PIEGINNTSKPGLWGGTVSTLEF----RVNVTQEMNGQ---VY-----TCQSANEAL-QRSAHEAVSLDVLYRPK-FVPPPSSTAVGVEGESLQVSLQTRANP-------------------TPVTYKW--TKDGTTIPQDGDHRIFAD---GGSLNFTRLHRDDAGIYSCSASNSQGGATLNITVV----------------VEYGTTIKSVSENIVVNPGEDAMLSCT--VEGKPLTEEHVKWERVGYDMTVKTSTTFANGTSYLHIKDAKREDVGNFRC-VADNRVDNPTNRDILLIVKFAPEIAKTPTLLRAASGTGERGRLPCRAQGSPKPQFIWRQDKKDLPINRTYKYEVEERKIDSLTYESTLIVDKVAPADYGAYECVARNELGEAVETVRLEITSQPDPPLSLNILNVTHDTVTLAWTPGFDGGLKASYRVRY 1003
BLAST of NPHS1-5 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087413) HSP 1 Score: 108.612 bits (270), Expect = 2.219e-23 Identity = 140/597 (23.45%), Postives = 240/597 (40.20%), Query Frame = 2
Query: 203 LQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWI-----VDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIP--QDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQMMILEDDIHWK---ENKNFFTQTSSVELKVNSAMHSSILICHVGEVD---KVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYI-DGIQIVKSKFSMRAISTRI-IIERKHDQCRIY-CQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISW 1945
+Q F TP D + + ++RC V N+ G+VQW +GF IP PR+ + DA ++ Y L I D+ +Y+CQV P +P +R L + PS I ++N + +++ ++++ + L CI+++ P W V + + N ++ P + S P D C ++AL D R VQ+ PG P T++ ++++C S G P A L W R +A S ++ S + +E NV E + NL+C++ L +A + +N T +Y L+ + Q K G+ C T+ ISW N + + D+ +K + + +S++ L ++S + I +CH + V S + VLY P ++ + L G +++ C+ GNP P L Y D I SK I++ + ++ D IY C+ + + +K + + P E + + PKN V G R + C + SNP ISW
Sbjct: 38 VQKFHLTPHD-LQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVIEGYARN-ARVEV---EERQNLTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVN--------QTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPPD---VPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACR-SRGGNPPAQLTW-------YRNGVAISSPQRTSGRLSE-----NVYKF-TAAAEDNGANLVCEAKNL---LATTPLRAELNLT----VLYAPKDVYLSGANQ-------AKVGDSVQLSCVTAPSNPQARISWSI--NGRPL---DNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTG--YNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKTLGVH---FPPETVKISVVPKNLV--PGIRAKLICDSSSSNPPAKISW 578 HSP 2 Score: 68.1662 bits (165), Expect = 5.464e-11 Identity = 55/233 (23.61%), Postives = 94/233 (40.34%), Query Frame = 2
Query: 2699 EDSTDLV-FYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDV----IETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESE 3382
E+ T L+ + + D G + C V A+ +L + PEI ++ + C S W + G D ++ S V E+A+ N S +G+Y C + N G+ T+E +P+ PD P V ++ T + W GFDGG F+++ + D + + KP ++ SL L P ++Y + A+N G S+
Sbjct: 790 ENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRL-KQHGEDKYKYVD-AKPGHQNISL----DGLKPGATYYFSVMAANEAGGSK 1016
BLAST of NPHS1-5 vs. Ensembl Fly
Match: hbs (gene:FBgn0029082 transcript:FBtr0087414) HSP 1 Score: 108.612 bits (270), Expect = 2.425e-23 Identity = 140/597 (23.45%), Postives = 240/597 (40.20%), Query Frame = 2
Query: 203 LQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWI-----VDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIP--QDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQMMILEDDIHWK---ENKNFFTQTSSVELKVNSAMHSSILICHVGEVD---KVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYI-DGIQIVKSKFSMRAISTRI-IIERKHDQCRIY-CQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISW 1945
+Q F TP D + + ++RC V N+ G+VQW +GF IP PR+ + DA ++ Y L I D+ +Y+CQV P +P +R L + PS I ++N + +++ ++++ + L CI+++ P W V + + N ++ P + S P D C ++AL D R VQ+ PG P T++ ++++C S G P A L W R +A S ++ S + +E NV E + NL+C++ L +A + +N T +Y L+ + Q K G+ C T+ ISW N + + D+ +K + + +S++ L ++S + I +CH + V S + VLY P ++ + L G +++ C+ GNP P L Y D I SK I++ + ++ D IY C+ + + +K + + P E + + PKN V G R + C + SNP ISW
Sbjct: 38 VQKFHLTPHD-LQILEGTDTLLRCEVSNRAGKVQWTKDGFALGFSAVIPGFPRYSVLVDAKQNAYNLQIKNATLEDDAEYQCQVGPAAGNPAIRANAKLSIVAAPSSIVIEGYARN-ARVEV---EERQNLTLHCIAENANPAAEIVWFQGEVPVSTAPIVTVN--------QTAPKRFTTSSTLHLQPRAEDDYKEFSCEARHKALPPD---VPMRAQVQLSVLYPPGAPFFEGYSQGETLHRGQEVQIACR-SRGGNPPAQLTW-------YRNGVAISSPQRTSGRLSE-----NVYKF-TAAAEDNGANLVCEAKNL---LATTPLRAELNLT----VLYAPKDVYLSGANQ-------AKVGDSVQLSCVTAPSNPQARISWSI--NGRPL---DNSTYKTTSSSDGGWVSSSNISLTIDSQSRTFIAVCHALNTELTQNVVGSHTVNVLYPPSPPLLTG--YNDGDILISGSILKLQCSSAGGNPPPTLQWYKNDKIINAPSKLVDSKITSELSLLVNASDNNAIYKCKVQNAAIDIPLFATKTLGVH---FPPETVKISVVPKNLV--PGIRAKLICDSSSSNPPAKISW 578 HSP 2 Score: 68.1662 bits (165), Expect = 5.336e-11 Identity = 55/233 (23.61%), Postives = 94/233 (40.34%), Query Frame = 2
Query: 2699 EDSTDLV-FYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDV----IETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESE 3382
E+ T L+ + + D G + C V A+ +L + PEI ++ + C S W + G D ++ S V E+A+ N S +G+Y C + N G+ T+E +P+ PD P V ++ T + W GFDGG F+++ + D + + KP ++ SL L P ++Y + A+N G S+
Sbjct: 790 ENGTALLQIASVERSDIGNFTCIVDNQRGAPAAQNVLLVVQTAPEIDHSPGFTRYAARLGVRAQLICRSLASPQPSFIWRRHGKDLKMQRRNKFKSVERQVDALNFESALLIENTSPDDYGQYECVVRNSLGQASTTLEFSKPSRPDAPLQLRVGNVSDTGVDLNWTPGFDGGMQTYFRLRL-KQHGEDKYKYVD-AKPGHQNISL----DGLKPGATYYFSVMAANEAGGSK 1016
BLAST of NPHS1-5 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 153.295 bits (386), Expect = 5.144e-37 Identity = 243/1089 (22.31%), Postives = 428/1089 (39.30%), Query Frame = 2
Query: 206 QCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQV-EPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDN-KFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKI-PQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSV-YLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISW-----EFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV---GEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIV----KSKFSMRAISTR-IIIERKHDQCRIY-CQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFY--SKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHS-DVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTD-LVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGC--DALSNCSVSCLFTLSGPADIHWFKDG---NDSRILYSTNITSNSSDVIETAIFFA-NISDA-QFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKI--QWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGES 3379
Q F+ P++ T +++C V G VQW + +G R++P PR+ +TGD + +Y L I + D+G YECQV P++ R L V S PY S+ + ++ ++ + CI+ P + D + LES ++ S S ++ + D + C N A P + T + ++ P P I + V L+V C +S G P A L W + E + F R R +VL + V + HA L CQS+ + +S +++ + + F P ++ LL S E +GE C TS I W E R +++ E D N T S++ KV+ + L C G + L V Y P+ + + + P+ +L G +++ C GNP L ++ ++V K S R +S ++I D Y C + ++ + ++K+ L P V VK + K + GQ + C SNP ISW L + K + K + M + S+L+ L+ + + C+ ++ + + N ++V+F P+ F + + ++++++ E T V ANP+E + C L+K P Y S W L I N + G +I SN G T +++D+ + ++ + V V++ +++ + + + + ++ W E S++D T L Y +++ G Y C S + L + P++ K RK D ++ +V C W K+G + Y T+ S+ I T+I N+S A + ++C N G+D ++++ PD P+D + ++ T T+ W GFDGG QIF++ QWA + + + P+ + Y + LNP++ Y+ ++A N GES
Sbjct: 72 QAFKTEPRN-VTVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPYFQLESE-----EPWVEGEQYTV--TCIAPDAKPPAEITMFRDGEELLES----ESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPA-----SPRALETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVC-MSYGGNPLATLHWTKNGEVLSTSWDVDFVSR----------RASSVLKMEVKPEDNHAV-LQCQSINQVTRSPMSLTRTLTVLFEPAEVT-------LLGSFE--------AVEGEEINLSCSTSSSNPPVHIRWWLGFKELNR-TEVTFAEGD-----NGGMMT-VSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAP--PPNVFLHSGSTLRLVCFSSGGNPTGQLT-WLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAKKVLSTQTKF----RVLFP-AVSVKIVA-KQKELRRGQTLDLDCLAGSSNPTANISW--SLGPVILKGVDQAPKRAEFGGMS-----------RTSKLSLTLESH----HWRKRITCQAFSNVLSEG-VNNFYSLDVLFPPE-FSDEQPDEVQVIEDETATLPAKVSANPDE-ITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEE------------------------SEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDLGNPRYEE--TTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSA---SYMYVDVFPPT----ATVYTVTGLNPSTMYNFSVNALNSIGES 1047
BLAST of NPHS1-5 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 152.91 bits (385), Expect = 6.270e-37 Identity = 243/1089 (22.31%), Postives = 428/1089 (39.30%), Query Frame = 2
Query: 206 QCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQV-EPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDN-KFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKI-PQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSV-YLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISW-----EFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV---GEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIV----KSKFSMRAISTR-IIIERKHDQCRIY-CQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFY--SKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHS-DVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTD-LVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGC--DALSNCSVSCLFTLSGPADIHWFKDG---NDSRILYSTNITSNSSDVIETAIFFA-NISDA-QFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKI--QWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGES 3379
Q F+ P++ T +++C V G VQW + +G R++P PR+ +TGD + +Y L I + D+G YECQV P++ R L V S PY S+ + ++ ++ + CI+ P + D + LES ++ S S ++ + D + C N A P + T + ++ P P I + V L+V C +S G P A L W + E + F R R +VL + V + HA L CQS+ + +S +++ + + F P ++ LL S E +GE C TS I W E R +++ E D N T S++ KV+ + L C G + L V Y P+ + + + P+ +L G +++ C GNP L ++ ++V K S R +S ++I D Y C + ++ + ++K+ L P V VK + K + GQ + C SNP ISW L + K + K + M + S+L+ L+ + + C+ ++ + + N ++V+F P+ F + + ++++++ E T V ANP+E + C L+K P Y S W L I N + G +I SN G T +++D+ + ++ + V V++ +++ + + + + ++ W E S++D T L Y +++ G Y C S + L + P++ K RK D ++ +V C W K+G + Y T+ S+ I T+I N+S A + ++C N G+D ++++ PD P+D + ++ T T+ W GFDGG QIF++ QWA + + + P+ + Y + LNP++ Y+ ++A N GES
Sbjct: 27 QAFKTEPRN-VTVRAGATALLKCEVLRASGAVQWVKDGLLLGPLRSLPGYPRYSMTGDQKKGQYHLQILDVTLEDDGPYECQVGRSESSRPIVSRTVWLNVQIPSSQPYFQLESE-----EPWVEGEQYTV--TCIAPDAKPPAEITMFRDGEELLES----ESYTMSGSQDKLQNTHAEVTIRARSSDDTRRLTCRAKNPA-----SPRALETGMTMKVYFPPQAPMIMGLENEEVKAGSFLKVVC-MSYGGNPLATLHWTKNGEVLSTSWDVDFVSR----------RASSVLKMEVKPEDNHAV-LQCQSINQVTRSPMSLTRTLTVLFEPAEVT-------LLGSFE--------AVEGEEINLSCSTSSSNPPVHIRWWLGFKELNR-TEVTFAEGD-----NGGMMT-VSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAP--PPNVFLHSGSTLRLVCFSSGGNPTGQLT-WLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAKKVLSTQTKF----RVLFP-AVSVKIVA-KQKELRRGQTLDLDCLAGSSNPTANISW--SLGPVILKGVDQAPKRAEFGGMS-----------RTSKLSLTLESH----HWRKRITCQAFSNVLSEG-VNNFYSLDVLFPPE-FSDEQPDEVQVIEDETATLPAKVSANPDE-ITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEE------------------------SEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDLGNPRYEE--TTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSA---SYMYVDVFPPT----ATVYTVTGLNPSTMYNFSVNALNSIGES 1002
BLAST of NPHS1-5 vs. Ensembl Zebrafish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 108.612 bits (270), Expect = 2.737e-23 Identity = 188/849 (22.14%), Postives = 334/849 (39.34%), Query Frame = 2
Query: 923 LRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSV-YLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISW-----EFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV---GEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIV----KSKFSMRAISTRII-IERKHDQCRIY-CQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFY--SKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHS-DVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTD-LVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGC--DALSNCSVSCLFTLSGPADIHWFKDG-----NDSRILYSTNITSNSSDVIETAIFFA-NISDA-QFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKI--QWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGES 3379
L+V C +S G P A L W + E + F R R +VL + V + HA L CQS+ + +S +++ + + F P ++ LL S E +GE C TS I W E R +++ E D N T S++ KV+ + L C G + L V Y P+ + + + P+ +L G +++ C GNP L ++ ++V K S R +S ++ I D Y C + ++ + ++K+ L P V VK + K + GQ + C SNP ISW L + K + K + M + S+L+ L+ + + C+ ++ + + N ++V+F P+ F + + ++++++ E T V ANP+E + C L+K P Y S W L I N + G +I SN G T +++D+ + ++ + V V++ +++ + + + + ++ W E S++D T L Y +++ G Y C S + L + P++ K RK D ++ +V C W K+G + R T+ S+ I T+I N+S A + ++C N G+D ++++ PD P+D + ++ T T+ W GFDGG QIF++ QWA + + + P+ + Y + LNP++ Y+ ++A N GES
Sbjct: 125 LKVVC-MSYGGNPLATLHWTKNGEVLSTSWDVDFVSR----------RASSVLKMEVKPEDNHAV-LQCQSINQVTRSPMSLTRTLTVLFEPAEVT-------LLGSFE--------AVEGEEINLSCSTSSSNPPVHIRWWLGFKELNR-TEVTFAEGD-----NGGMMT-VSNLTHKVSREDNGLSLQCEAFNKGTRFSSVRAAELIVYYPPQKVWLDAP--PPNVFLHSGSTLRLVCFSSGGNPTGQLT-WLKNNKVVLTASKQVSSERGVSRELLLILHPSDNLATYRCDSSNKAKKVLSTQTKF----RVLFP-AVSVKIVA-KQKELRRGQTLDLDCLAGSSNPTANISW--SLGPVILKGVDQAPKRAEFGGMS-----------RTSKLSLTLESH----HWRKRITCQAFSNVLSEGV-NNFYSLDVLFPPE-FSDEQPDEVQVIEDETATLPAKVSANPDE-ITCEWIFQGEKLVKERDPRYTFSDWTLEIVNVSRRDGGDYIIECSNAEGSNRTKLRLDVQYSPTVRMKSDPVFVNIGDTADLLCVADANPITSSMFSWKWMGEGERDELGEE------------------------SEDDGTGVLTIYEVTRDRAGVYQCTADNGISPTGSVEGQLIVRFAPDLQKGPQWRKVASRGDGSNDANVVCQAQGVPRVQFRWAKNGFPLDLGNPRY----EETTESTGTIHTSILTVINVSAALDYAIFTCTAHNSMGEDSLDIQLLSTNHPDPPSDLKLLRVDQTSVTLEWMPGFDGGLTQIFRVRYQWAGSA---SYMYVDVFPPT----ATVYTVTGLNPSTMYNFSVNALNSIGES 875
BLAST of NPHS1-5 vs. Ensembl Zebrafish
Match: kirrel1b (kirre like nephrin family adhesion molecule 1b [Source:ZFIN;Acc:ZDB-GENE-070912-173]) HSP 1 Score: 68.5514 bits (166), Expect = 4.465e-11 Identity = 63/264 (23.86%), Postives = 101/264 (38.26%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPG-MPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESI 1000
F + P D+ ++ + VV+ C V N G VQW + +G + A PR+R+ +Y L I +D+ YECQ L RR L V P P I + +IL+ +L C+S+ P+ T W D +E + S E SD + S + P C+ SN A P R+ V + P + I V +R + +PP ++ ++W+ +
Sbjct: 27 FSQEPADQ-SVVIGERVVLSCVVFNYTGIVQWTKDGLALGIGEDLRAWPRYRVLRIMDVGQYNLEITSADLTDDSLYECQA---TEAALRSRRAKLTVLIPPDGPVI------EGSPEILL-TAGTSFNLTCVSRGAKPMSTIEWYKDGIIVEGAH---TSTEVLSDRKRVTTKSFLEIQPMDTDTGRNFTCVASNLA-----APLGKRSTVTLNIHHPPTVILSIEPRSVLEGERVKFTCQATANPP-----IMGYRWAKGGV 266
BLAST of NPHS1-5 vs. Ensembl Zebrafish
Match: kirrel1b (kirre like nephrin family adhesion molecule 1b [Source:ZFIN;Acc:ZDB-GENE-070912-173]) HSP 1 Score: 68.5514 bits (166), Expect = 4.503e-11 Identity = 63/264 (23.86%), Postives = 101/264 (38.26%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPG-MPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESI 1000
F + P D+ ++ + VV+ C V N G VQW + +G + A PR+R+ +Y L I +D+ YECQ L RR L V P P I + +IL+ +L C+S+ P+ T W D +E + S E SD + S + P C+ SN A P R+ V + P + I V +R + +PP ++ ++W+ +
Sbjct: 27 FSQEPADQ-SVVIGERVVLSCVVFNYTGIVQWTKDGLALGIGEDLRAWPRYRVLRIMDVGQYNLEITSADLTDDSLYECQA---TEAALRSRRAKLTVLIPPDGPVI------EGSPEILL-TAGTSFNLTCVSRGAKPMSTIEWYKDGIIVEGAH---TSTEVLSDRKRVTTKSFLEIQPMDTDTGRNFTCVASNLA-----APLGKRSTVTLNIHHPPTVILSIEPRSVLEGERVKFTCQATANPP-----IMGYRWAKGGV 266
BLAST of NPHS1-5 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 70.4774 bits (171), Expect = 1.236e-11 Identity = 54/199 (27.14%), Postives = 81/199 (40.70%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRAL 808
F + P DK + +V + C V G VQW + +G +R +P R+ + GD S E+ L I ++ SD+ YECQ L +R L V P P I V+N+ + + +L C++ P +W D K L+S + E SD + S PS C + N AL
Sbjct: 26 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQA---TQAALRSQRARLTVLLPPGDPMI--PGSPVANVLLNVPY-----NLTCLAPVAKPAAEITWYRDGKKLDS---AVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEAL 210
BLAST of NPHS1-5 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 70.4774 bits (171), Expect = 1.348e-11 Identity = 54/199 (27.14%), Postives = 81/199 (40.70%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRAL 808
F + P DK + +V + C V G VQW + +G +R +P R+ + GD S E+ L I ++ SD+ YECQ L +R L V P P I V+N+ + + +L C++ P +W D K L+S + E SD + S PS C + N AL
Sbjct: 17 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQA---TQAALRSQRARLTVLLPPGDPMI--PGSPVANVLLNVPY-----NLTCLAPVAKPAAEITWYRDGKKLDS---AVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEAL 201
BLAST of NPHS1-5 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 70.0922 bits (170), Expect = 1.851e-11 Identity = 54/199 (27.14%), Postives = 81/199 (40.70%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRAL 808
F + P DK + +V + C V G VQW + +G +R +P R+ + GD S E+ L I ++ SD+ YECQ L +R L V P P I V+N+ + + +L C++ P +W D K L+S + E SD + S PS C + N AL
Sbjct: 38 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQA---TQAALRSQRARLTVLLPPGDPMI--PGSPVANVLLNVPY-----NLTCLAPVAKPAAEITWYRDGKKLDS---AVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEAL 222
BLAST of NPHS1-5 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 70.0922 bits (170), Expect = 1.901e-11 Identity = 54/199 (27.14%), Postives = 81/199 (40.70%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRAL 808
F + P DK + +V + C V G VQW + +G +R +P R+ + GD S E+ L I ++ SD+ YECQ L +R L V P P I V+N+ + + +L C++ P +W D K L+S + E SD + S PS C + N AL
Sbjct: 30 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQA---TQAALRSQRARLTVLLPPGDPMI--PGSPVANVLLNVPY-----NLTCLAPVAKPAAEITWYRDGKKLDS---AVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEAL 214
BLAST of NPHS1-5 vs. Ensembl Xenopus
Match: eif4e (eukaryotic translation initiation factor 4E [Source:Xenbase;Acc:XB-GENE-855435]) HSP 1 Score: 69.707 bits (169), Expect = 2.308e-11 Identity = 54/199 (27.14%), Postives = 81/199 (40.70%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRAL 808
F + P DK + +V + C V G VQW + +G +R +P R+ + GD S E+ L I ++ SD+ YECQ L +R L V P P I V+N+ + + +L C++ P +W D K L+S + E SD + S PS C + N AL
Sbjct: 30 FSQQPSDKV-IVAGKSVTLPCVVIGYRGVVQWTMDGLALGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQA---TQAALRSQRARLTVLLPPGDPMI--PGSPVANVLLNVP-----YNLTCLAPVAKPAAEITWYRDGKKLDS---AVYTKELLSDGKSEGSASSLLITPSLADSGSSYTCRVRNEAL 214
BLAST of NPHS1-5 vs. Ensembl Mouse
Match: Kirrel (kirre like nephrin family adhesion molecule 1 [Source:MGI Symbol;Acc:MGI:1891396]) HSP 1 Score: 66.6254 bits (161), Expect = 1.428e-10 Identity = 49/199 (24.62%), Postives = 75/199 (37.69%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRAL 808
F + P D+ + V+ C + N G VQW + +G + + A PR+R+ G A +Y L I + SD+ YECQ L RR L V P + +++ Q +L C + + P T W D E S E D ++ S P+ L + C N A+
Sbjct: 55 FSQEPADQ-TVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQA---TEAALRSRRAKLTVLIPP-------EETRIDGGPVILLQAGTPYNLTCRAFNAKPAATIIWFRDGTQQEG---AVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEAI 239
BLAST of NPHS1-5 vs. Ensembl Mouse
Match: Kirrel (kirre like nephrin family adhesion molecule 1 [Source:MGI Symbol;Acc:MGI:1891396]) HSP 1 Score: 65.0846 bits (157), Expect = 5.888e-10 Identity = 72/330 (21.82%), Postives = 116/330 (35.15%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMP-KIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPR 1198
F + P D+ + V+ C + N G VQW + +G + + A PR+R+ G A +Y L I + SD+ YECQ L RR L V P + +++ Q +L C + + P T W D E S E D ++ S P+ L + C N A+ P+ T +++ P + I V +R + +P +L ++W+ F + E N + PV C+ VY NVS + ++F PR
Sbjct: 55 FSQEPADQ-TVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQA---TEAALRSRRAKLTVLIPP-------EETRIDGGPVILLQAGTPYNLTCRAFNAKPAATIIWFRDGTQQEG---AVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEAI-----PNGKETSIELDVHHPPTVTLSIEPQTVLEGERVIFTCQATANP-----EILGYRWAKGG--------FLIEDAHESRYETNVDYSFFTEPVS----------CE-VYNKVGSTNVSTLVNVHFAPR 341
BLAST of NPHS1-5 vs. Ensembl Mouse
Match: Kirrel (kirre like nephrin family adhesion molecule 1 [Source:MGI Symbol;Acc:MGI:1891396]) HSP 1 Score: 65.0846 bits (157), Expect = 5.888e-10 Identity = 72/330 (21.82%), Postives = 116/330 (35.15%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMP-KIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPR 1198
F + P D+ + V+ C + N G VQW + +G + + A PR+R+ G A +Y L I + SD+ YECQ L RR L V P + +++ Q +L C + + P T W D E S E D ++ S P+ L + C N A+ P+ T +++ P + I V +R + +P +L ++W+ F + E N + PV C+ VY NVS + ++F PR
Sbjct: 55 FSQEPADQ-TVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQA---TEAALRSRRAKLTVLIPP-------EETRIDGGPVILLQAGTPYNLTCRAFNAKPAATIIWFRDGTQQEG---AVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEAI-----PNGKETSIELDVHHPPTVTLSIEPQTVLEGERVIFTCQATANP-----EILGYRWAKGG--------FLIEDAHESRYETNVDYSFFTEPVS----------CE-VYNKVGSTNVSTLVNVHFAPR 341
BLAST of NPHS1-5 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 62.3882 bits (150), Expect = 4.387e-9 Identity = 75/343 (21.87%), Postives = 141/343 (41.11%), Query Frame = 2
Query: 245 IVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHP-LLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQ-DKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSV-YLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSI 1264
+ TV + C V VQW + +G + IP PR+ + GD+++ E+ L I SD+ +YECQV + P L+ L + P ++ + + + Q+ + C+S P P +I + +E + + NE + + + + + P + L+ C SN ALA P + I F P + P ++ V ++L + C ++ G P A L+W ++ K + +A+ T + + + + V E H L CQS ++ S+ + + F P + I + + SE K+ ++
Sbjct: 58 VEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVILSILVSPK---VLQLTPEAGSTVTWVAGQEYVV--TCVSGDAKPAPDIIFIQGGRTVE--DVSSSVNEGSEEKLFFT-EAEARVTPQSSDNGQLLVCEGSNPALAT---PIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPC-IARGGNPPATLQWL---KNGKPVSIAW--------GTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILGST----SQSENKNVTL 373 HSP 2 Score: 58.9214 bits (141), Expect = 5.144e-8 Identity = 64/266 (24.06%), Postives = 107/266 (40.23%), Query Frame = 2
Query: 2687 KCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYI-LSILMQPEIVKYQSIRKFGC--DALSNCSVSCLFTLSGPADIHWFKDG-----NDSRILYSTNITSNSSDVIETAIFFANISDAQ-FGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQ 3457
K SK + L G Y C V +G+ A+ + L + P++ + K D+ S+ ++ C D W K+G D R T + V + + AN+S AQ + + C +N G D +++V + PD P V S+ + W+ GFDGG Q F+I++ + + + L Q+ ++ + L P++ Y I + ASN G+S Q + T L DQ
Sbjct: 806 KMSKGSTGRLRIRQAKLSQAGAYQCIVD-NGVAPAARGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRY---TEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVGLEWKPGFDGGLPQRFQIRYEA-LESPGFLYMDVLP----AQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQV-SITTPGLDQAPEDTDQ 1061
BLAST of NPHS1-5 vs. Ensembl Mouse
Match: Nphs1 (nephrosis 1, nephrin [Source:MGI Symbol;Acc:MGI:1859637]) HSP 1 Score: 62.003 bits (149), Expect = 5.360e-9 Identity = 75/343 (21.87%), Postives = 141/343 (41.11%), Query Frame = 2
Query: 245 IVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHP-LLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQ-DKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSV-YLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSI 1264
+ TV + C V VQW + +G + IP PR+ + GD+++ E+ L I SD+ +YECQV + P L+ L + P ++ + + + Q+ + C+S P P +I + +E + + NE + + + + + P + L+ C SN ALA P + I F P + P ++ V ++L + C ++ G P A L+W ++ K + +A+ T + + + + V E H L CQS ++ S+ + + F P + I + + SE K+ ++
Sbjct: 44 VEGSTVKLWCGVRAPGSVVQWAKDGLLLGPNPKIPGFPRYSLEGDSAKGEFHLLIEACDLSDDAEYECQVGRSELGPELVSPSVILSILVSPK---VLQLTPEAGSTVTWVAGQEYVV--TCVSGDAKPAPDIIFIQGGRTVE--DVSSSVNEGSEEKLFFT-EAEARVTPQSSDNGQLLVCEGSNPALAT---PIKASFTMNILFPPGPPVIDWPGLNEGHVRAGENLELPC-IARGGNPPATLQWL---KNGKPVSIAW--------GTEHAQAVAHSVLVMTVRPEDHGARLSCQSYNSVSAETQERSITLQVTFPPSAVTILGST----SQSENKNVTL 359 HSP 2 Score: 58.5362 bits (140), Expect = 5.631e-8 Identity = 64/266 (24.06%), Postives = 107/266 (40.23%), Query Frame = 2
Query: 2687 KCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYI-LSILMQPEIVKYQSIRKFGC--DALSNCSVSCLFTLSGPADIHWFKDG-----NDSRILYSTNITSNSSDVIETAIFFANISDAQ-FGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQ 3457
K SK + L G Y C V +G+ A+ + L + P++ + K D+ S+ ++ C D W K+G D R T + V + + AN+S AQ + + C +N G D +++V + PD P V S+ + W+ GFDGG Q F+I++ + + + L Q+ ++ + L P++ Y I + ASN G+S Q + T L DQ
Sbjct: 792 KMSKGSTGRLRIRQAKLSQAGAYQCIVD-NGVAPAARGLVRLVVRFAPQVDHPTPLTKVAAAGDSTSSATLHCRARGVPNIDFTWTKNGVPLDLQDPRY---TEHKYHQGVVHSSLLTIANVSAAQDYALFKCTATNALGSDHTNIQLVSISRPDPPLGLKVVSVSPHSVGLEWKPGFDGGLPQRFQIRYEA-LESPGFLYMDVLP----AQATTFTLTGLKPSTRYRIWLLASNALGDSGLTDKGIQV-SITTPGLDQAPEDTDQ 1047
BLAST of NPHS1-5 vs. UniProt/SwissProt
Match: sp|B1Q236|SYG1_CAEEL (Synaptogenesis protein syg-1 OS=Caenorhabditis elegans OX=6239 GN=syg-1 PE=1 SV=1) HSP 1 Score: 78.1814 bits (191), Expect = 3.648e-13 Identity = 70/304 (23.03%), Postives = 120/304 (39.47%), Query Frame = 2
Query: 218 ETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRA--IPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKH-HPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVD----------------NKFL------------------ESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRA----LALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKR 1006
E P+D + V +T ++ C V++Q G VQW + +G DR +P R+R+ G A+ EY L I + D+ D+ CQ+ H ++ + L V +P+ P IV + ++ + I C+S+ G P PT W + +KF E+ ++ N SR D S S S+ F P D + CI + + +DS S R QI +P + + S ++C+++ L + W N+ ++
Sbjct: 27 EAPKDTLAA-VGETAILTCRVEHQQGPVQWMKDDFGLGTDRDKPLPGNKRYRMVGSAANGEYNLEISNVTLFDDDDFACQISESDHAKAVVSSKAKLTVLVRPTPPKIVKSHHSLKAI------AGDPITQSCLSRKGKPPPTIGWAIASDEHGKHIVSWLGESRSKFGGIHAKPEISQETVIAHVNETTQVEEGGNNSREDSSIYSIMSNLSFIPRPEDDHKYLICISQHMTFPNKIEVDSVKLSLRYAPQINLTVASKLP--------LRENGSALLACNVNAKPLDNVKISWYKGNQKLRE 315
BLAST of NPHS1-5 vs. UniProt/SwissProt
Match: sp|Q08180|ICCR_DROME (Irregular chiasm C-roughest protein OS=Drosophila melanogaster OX=7227 GN=rst PE=1 SV=2) HSP 1 Score: 75.8702 bits (185), Expect = 1.788e-12 Identity = 43/150 (28.67%), Postives = 69/150 (46.00%), Query Frame = 2
Query: 206 QCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPI-KHHPLLRRR-THLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWI 649
Q F PQD+ ++V V + C V N+ G +QW + +G R + R+ + G +Y L IYP+ D+ Y+CQV P + P +R L V P P I + D++ + + + +EC+S G P +WI
Sbjct: 28 QRFAMEPQDQ-TAVVGARVTLPCRVINKQGTLQWTKDDFGLGTSRDLSGFERYAMVGSDEEGDYSLDIYPVMLDDDARYQCQVSPGPEGQPAIRSTFAGLTVLVPPEAPKI-------TQGDVIYATEDRKVEIECVSVGGKPAAEITWI 169
BLAST of NPHS1-5 vs. UniProt/SwissProt
Match: sp|O60500|NPHN_HUMAN (Nephrin OS=Homo sapiens OX=9606 GN=NPHS1 PE=1 SV=1) HSP 1 Score: 71.2478 bits (173), Expect = 5.448e-11 Identity = 62/247 (25.10%), Postives = 105/247 (42.51%), Query Frame = 2
Query: 245 IVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHP-LLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQ-DKVTVNDRDSLRVSCSLSPPGLPKALLEW 979
+ +V +RC V VQW + +G D IP PR+R+ GD +R E+ L I SD+ +YECQV + P L+ R L + P ++ + M + Q+ ++ C+S P P + ++ + + + N NE + + + P ++ L+ C S+ AL P V + F P + + P D+ V SL + C ++ G P A L+W
Sbjct: 44 VEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPK---LLLLTPEAGTMVTWVAGQEYVVN--CVSGDAKPAPDITILLSGQTISDISA--NVNEGSQQKLFTV-EATARVTPRSSDNRQLLVCEASSPAL---EAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPC-VARGGNPLATLQW 278 HSP 2 Score: 63.1586 bits (152), Expect = 1.842e-8 Identity = 58/241 (24.07%), Postives = 105/241 (43.57%), Query Frame = 2
Query: 2687 KCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQ--PEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADI--HWFKDG-----NDSRILYSTNITSNSSDVIETAIFFANISDAQ-FGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGES 3379
K S+ + L ++ G Y C VD + + +L ++++ P++ + K S S + G +I W K+G D R T T + V + + AN+S AQ + ++C +N G D +++V + PD P+ V S+ + W+ GFDGG Q F I++ + +H + P Q+ ++ + L P++ Y + + ASN G+S
Sbjct: 792 KISRGPTGRLRIHHAKLAQAGAYQC--IVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRY---TEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRY--EALGTPGFHYVDVVPP---QATTFTLTGLQPSTRYRVWLLASNALGDS 1022
BLAST of NPHS1-5 vs. UniProt/SwissProt
Match: sp|Q96J84|KIRR1_HUMAN (Kin of IRRE-like protein 1 OS=Homo sapiens OX=9606 GN=KIRREL1 PE=1 SV=2) HSP 1 Score: 67.3958 bits (163), Expect = 7.024e-10 Identity = 54/220 (24.55%), Postives = 83/220 (37.73%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRP 871
F + P D+ + V+ C + N G VQW + +G + + A PR+R+ G A +Y L I + SD+ YECQ L RR L V P + +++ Q +L C + + P T W D E S E D ++ S NP+ L + C N A+ PS T +++ P
Sbjct: 23 FSQEPADQ-TVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQA---TEAALRSRRAKLTVLIPP-------EDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVA---STELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAI-----PSGKETSIELDVHHPP 223
BLAST of NPHS1-5 vs. UniProt/SwissProt
Match: sp|Q80W68|KIRR1_MOUSE (Kin of IRRE-like protein 1 OS=Mus musculus OX=10090 GN=Kirrel1 PE=1 SV=1) HSP 1 Score: 65.0846 bits (157), Expect = 4.118e-9 Identity = 72/330 (21.82%), Postives = 116/330 (35.15%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMP-KIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPR 1198
F + P D+ + V+ C + N G VQW + +G + + A PR+R+ G A +Y L I + SD+ YECQ L RR L V P + +++ Q +L C + + P T W D E S E D ++ S P+ L + C N A+ P+ T +++ P + I V +R + +P +L ++W+ F + E N + PV C+ VY NVS + ++F PR
Sbjct: 55 FSQEPADQ-TVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQA---TEAALRSRRAKLTVLIPP-------EETRIDGGPVILLQAGTPYNLTCRAFNAKPAATIIWFRDGTQQEG---AVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEAI-----PNGKETSIELDVHHPPTVTLSIEPQTVLEGERVIFTCQATANP-----EILGYRWAKGG--------FLIEDAHESRYETNVDYSFFTEPVS----------CE-VYNKVGSTNVSTLVNVHFAPR 341
BLAST of NPHS1-5 vs. TrEMBL
Match: A0A0H3YJK2 (NPHS1-5 OS=Schmidtea mediterranea OX=79327 GN=NPHS1-5 PE=2 SV=1) HSP 1 Score: 2485.29 bits (6440), Expect = 0.000e+0 Identity = 1224/1224 (100.00%), Postives = 1224/1224 (100.00%), Query Frame = 2
Query: 77 MPSRLAWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHVGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQYLKVLAYXXXXXXXXXXXXXXXXXXXKVNSSMKDRRSLHHKSMTHFISLNNRLYSSMIEDNKDISMIFTNRISSSPENSRLNDSGRDSLVNISDNA 3748
MPSRLAWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHVGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQYLKVLAYTLAICSLAILLIIIITGLLKVNSSMKDRRSLHHKSMTHFISLNNRLYSSMIEDNKDISMIFTNRISSSPENSRLNDSGRDSLVNISDNA
Sbjct: 1 MPSRLAWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHVGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQYLKVLAYTLAICSLAILLIIIITGLLKVNSSMKDRRSLHHKSMTHFISLNNRLYSSMIEDNKDISMIFTNRISSSPENSRLNDSGRDSLVNISDNA 1224
BLAST of NPHS1-5 vs. TrEMBL
Match: A0A0H3YFQ6 (NPHS1-6 OS=Schmidtea mediterranea OX=79327 GN=NPHS1-6 PE=2 SV=1) HSP 1 Score: 348.977 bits (894), Expect = 1.523e-95 Identity = 308/1176 (26.19%), Postives = 518/1176 (44.05%), Query Frame = 2
Query: 146 GAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPI-KHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLE--CISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPS--SYRTFVQIRFQKRPGMPKI----PQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFS-------LRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDF---SILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQM---MILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV-GEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAIST----RIIIER--KHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQF--KEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYN--YHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPE-EDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWS--LRRMDHNFIET-------ILGCNRGL-----YFNNATSRY-------------LKLFVKC----SKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYI-LSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRI----LYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTS-WHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQ 3460
GAK I QS +C+ E+I R+QCF+E P+ YE +RC V NQ+G+VQWRA V +G+DR IP +P +R+ G + E+FL I + D G YECQV+P+ + HPLLRR+T L V +P P I Y + ++I+ K+ + E C+S +G P + W ++N ++ DN N + + S S SK +D +++C +SNRA + P S T V I PG P+I P D++ ++C SP G P L W+W ++ + F QY +T N++V+ L +P + + H L C + S + +V + I P K+ I ++G L + + KD +++ + GE C T YY TI W F N + I + WKE+++ F + S++++K+N S L C + V + + L + Y P I V + + ++ C+ GNP P+L + D + ++S F + + +III ++ + +CQA S + S + L + P + +K + VE+G+ + CT SNP V+ W+L C + + + ++ +C + + SN S + S + PSW H V C+V+N Y ++++ I INV F P+ F + +G+ +T ++ IANP +D +S ++ SK L I N+ V +V N G + I ++I + + + + CS S+ N ++++ W+ + +N I + +GCN+ L Y N Y K F KC E ++ L+ Y + + D G Y C V +G+ K I L+ P+I+ K + S+ C + WF ++ + Y NIT + + N+ G Y C+ N G D +EV + PD P + V + + I W QGFDGG +Q F+++W K TS + E + + +LS++I L +++Y I I++ N S+ TS F +K +++S+ + + Q
Sbjct: 45 GAK-IKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQ-----DNENSALLEELNYS-RSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDES-FRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNV--GKPVKLNHNFEMTCSTIGGNPYPELQWFRDS-KPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSL--SGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQ-FEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRC-VVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKE--KSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRN----SKHGTSQF-SKTIMTNTMSADNEKENNQ 1198
BLAST of NPHS1-5 vs. TrEMBL
Match: G7YEL7 (Nephrin OS=Clonorchis sinensis OX=79923 GN=CLF_106049 PE=4 SV=1) HSP 1 Score: 343.584 bits (880), Expect = 1.134e-93 Identity = 297/1225 (24.24%), Postives = 521/1225 (42.53%), Query Frame = 2
Query: 128 NLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPI--KHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLE--CISKHGFPIPTYSWIVDNKFLESFNCK-------DNSNESRSDP----SYKSPPSHFKFNPSKLQDQLLVECIISNRALAL--DSGPSSYRTFVQIRFQKRPGMPKI--PQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIK----------------RIPLAYFSLRQYSVKTTEN--------------------------------NRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPL---LASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQ-------RNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV-GEVD-KVSASIHLEVLYFPRVINISS-SIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSK-----FSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTK--QFKEIKKS---------------CQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDV--IANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLL--------------IVASNTMGRTST--FIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLW------SLRRMDHNFIETILG----CNRGLYFNNATSRYLKLFVKCSKEDSTDLV----FYYISQGDNGRYVCNVTVDGLLVASTKYILSIL-MQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDG---NDSRILYSTNITSNSS--------DVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASN 3364
L ++ A ++QSS C+P++ + R+QCF PQD+Y + +TV M+C V NQHG+VQWRAK++ +G+DR++P PR+RI GD R ++ L I +Q D G+YECQV P+ +HPLLRR+T L V KPS P I+Y + ++I Q + + C++ G P P+++WI++N+ + + + D ++ DP + S + L+D + C ++N A L D + T V IR PG P+I P+ K + + L+ C SPPG P L W+W + + + +S + +KT E+ ++++N L +P + HA L+C++ + S S+ I + + P + I G + L S ++ S++ + GEI C+TS Y+ I W Q + + + + I + + Q S V++K++ + C V E D +V A + ++++Y P V I+ + P + + +++ C GNP P+LA + D Q+ S+ ++ +++ +R + +C A + ++ + S+ + L++ P+ V V + P VV SGQ ISC SNP +I+W C QFK K + C+ + ++ N + S L +P+W YH + C+ N + + + + +N+ F P+ F + +K +++ EG T +D+ +NP V S + I P + T + G LL +VA N + +S F+ V A + +E V + S +V C ++ +F W D N E L CN+ + +Y F+ C ++DS + Y I++ D G YVC + +GL K I + P IV+ K + S +++CL I W K G +DS+ S T + ++ + N+ FG Y C+ N G+ + V + + PD P + + + + W QGFDGG Q F+++W + + + ++ ++ YLI L + Y + +++ N
Sbjct: 32 GLLQVSDAARVIQSSACTPQERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIISQPDPIVRITVLCVTVGGSPPPSFTWIINNQEIPTKPLQTSAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCGVTNAATQLHHDIQQRNLTTQVMIRVHTPPGPPRIISPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPAQVGDQKTGRIIGFGGRGGARMNEYSAVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPVGSAHQTSIEIFVKYAPANVTISVEGGSVREELVSGRRE--SVVYGQAGEIKTLICRTSPYFKQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDVKISDEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRNLQP---VKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNSEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASEAVVLNVVFPPQGVSVNFHGPS--VVPSGQPLVISCQADSSNPVAIINWWHFRCGPAHAFSQFKASKTTVDEVLYERRTPERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRTENPEYGPPVWHDRLQLNITFAPQ-FLGLQTRKEHVIR-EGTTVNLDLGPYSNPP-----VTSVSWFVEGRPIPPETAPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAIFFLNVTFPASLAGAAEENVTQTTSDQVTLV-CQATANPSIAIQSFTWYRYVPPPGWPQDVNEAEVQLSAPMPCNQSDAGPSVDDKY---FIHCQRDDSVHMTSRFSIYRINRDDVGTYVCEIN-NGLGDPVRKNIHFVYHFAPGIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGWLKAGQIIHDSQREGSGASTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLEVSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLATGSNNMFKYADVAANDNHHAVEYLITGLESGAEYQVSVNSMN 1237
BLAST of NPHS1-5 vs. TrEMBL
Match: A0A4S2LBK4 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 340.502 bits (872), Expect = 1.047e-92 Identity = 300/1218 (24.63%), Postives = 515/1218 (42.28%), Query Frame = 2
Query: 149 AKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPI--KHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLE--CISKHGFPIPTYSWIVDN-----KFLESFNCK--DNSNESRSDP----SYKSPPSHFKFNPSKLQDQLLVECIISNRALAL--DSGPSSYRTFVQIRFQKRPGMPKI--PQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKW-----------SNESIKRIPLAYFSLRQYS-----VKTTEN--------------------------------NRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPL---LASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQ-------RNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV-GEVD-KVSASIHLEVLYFPRVINISS-SIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSK-----FSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTK--QFKEIKKS---------------CQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDV--IANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLL--------------IVASNTMGRTST--FIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLW------SLRRMDHNFIETILG----CNRGLYFNNATSRYLKLFVKCSKEDS----TDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSIL-MQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGN---DSRILYSTNITSNSS--------DVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASN 3364
A ++QSS C+P + + R+QCF PQD+Y + +TV M+C V NQHG+VQWRAK++ +G+DR++P PR+RI GD R ++ L I +Q D G+YECQV P+ +HPLLRR+T L V KPS P I+Y + ++I Q + + C + G P P+++WI++N K L++ + D ++ DP + S + L+D + C ++N A L D + T V IR PG P+I P+ K + + L+ C SPPG P L W+W + I + +YS +KT E+ ++++N L +P + HA L+C++ + S S+ I + + P + I G + L S ++ S++ + GEI C+TS Y+ I W Q + + + + I + + Q S V++K++ + C V E D +V A + ++++Y P V I+ + P + + +++ C GNP P+LA + D Q+ ++ ++ +++ +R + +C A + ++ + S + L++ P+ V V + P VV SGQ ISC SNP I+W C QFK K + C+ + ++ N + S L +P+W YH + C+ N + + + + +N+ F P+ F + +K ++++ EG T +D+ +NP V S + I P T + G LL +VA N + +S F+ V A + +E V + S +V C ++ F W D N E L CN+ + +Y F+ C ++DS + L Y I++ D G YVC + +GL K I + P IV+ K + S +++CL I W K G DS+ S +T + ++ + N+ FG Y C+ N G+ + V + + PD P + + + + W QGFDGG Q F+++W + + + ++ ++ YLI L + Y + +++ N
Sbjct: 40 AARVIQSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIISQPDPIVRITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQRNLTTQVVIRVHTPPGPPRIISPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPVGSAHQTSIEIFVKYAPANVTISVDGGSVREELVSGRRE--SVVYGQAGEIKTLICRTSPYFKQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDVKISEEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRSLQP---VKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASDAVVLNVVFPPQGVSVNFHGPS--VVPSGQPLVISCQADSSNPVATINWWHFRCGPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRTENPEYGPPVWHDRLQLNITFAPQ-FLGLQTRKEQVIR-EGTTVNLDLGPYSNPP-----VTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVTQTTSDQVTLV-CQATANPSIATQAFTWYRYVPPPGWPQDVNEAEVQLSAPMPCNQSDASPSVDDKY---FIHCQRDDSIHMTSRLSIYRINRDDVGTYVCEIN-NGLGDPVRKNIHFVYHFTPSIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGWLKAGQMIYDSQREGSGALTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLEVSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLATGSNNMFKYADVAANDNHHAVEYLITGLESGAEYQVSVNSMN 1238
BLAST of NPHS1-5 vs. TrEMBL
Match: A0A4S2LB20 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008491 PE=4 SV=1) HSP 1 Score: 340.117 bits (871), Expect = 1.407e-92 Identity = 300/1218 (24.63%), Postives = 515/1218 (42.28%), Query Frame = 2
Query: 149 AKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPI--KHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLE--CISKHGFPIPTYSWIVDN-----KFLESFNCK--DNSNESRSDP----SYKSPPSHFKFNPSKLQDQLLVECIISNRALAL--DSGPSSYRTFVQIRFQKRPGMPKI--PQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKW-----------SNESIKRIPLAYFSLRQYS-----VKTTEN--------------------------------NRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPL---LASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQ-------RNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV-GEVD-KVSASIHLEVLYFPRVINISS-SIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSK-----FSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTK--QFKEIKKS---------------CQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDV--IANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLL--------------IVASNTMGRTST--FIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLW------SLRRMDHNFIETILG----CNRGLYFNNATSRYLKLFVKCSKEDS----TDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSIL-MQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGN---DSRILYSTNITSNSS--------DVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASN 3364
A ++QSS C+P + + R+QCF PQD+Y + +TV M+C V NQHG+VQWRAK++ +G+DR++P PR+RI GD R ++ L I +Q D G+YECQV P+ +HPLLRR+T L V KPS P I+Y + ++I Q + + C + G P P+++WI++N K L++ + D ++ DP + S + L+D + C ++N A L D + T V IR PG P+I P+ K + + L+ C SPPG P L W+W + I + +YS +KT E+ ++++N L +P + HA L+C++ + S S+ I + + P + I G + L S ++ S++ + GEI C+TS Y+ I W Q + + + + I + + Q S V++K++ + C V E D +V A + ++++Y P V I+ + P + + +++ C GNP P+LA + D Q+ ++ ++ +++ +R + +C A + ++ + S + L++ P+ V V + P VV SGQ ISC SNP I+W C QFK K + C+ + ++ N + S L +P+W YH + C+ N + + + + +N+ F P+ F + +K ++++ EG T +D+ +NP V S + I P T + G LL +VA N + +S F+ V A + +E V + S +V C ++ F W D N E L CN+ + +Y F+ C ++DS + L Y I++ D G YVC + +GL K I + P IV+ K + S +++CL I W K G DS+ S +T + ++ + N+ FG Y C+ N G+ + V + + PD P + + + + W QGFDGG Q F+++W + + + ++ ++ YLI L + Y + +++ N
Sbjct: 40 AARVIQSSACTPRERQDRIQCFDVLPQDQYSVPLGNTVNMQCVVLNQHGKVQWRAKKILLGYDRSLPGYPRYRIIGDVGRGQHTLQIMDVQLEDAGEYECQVTPVPANNHPLLRRKTELVVLVKPSVPRILYPDVPPPDNQLIISQPDPIVRITVLCATVGGSPAPSFTWIINNQEIPMKPLQASAGRHPDRGPWAKLDPIINTETEKQESRLSLLKAGLKDGDQLTCSVTNAATQLHHDIQQRNLTTQVVIRVHTPPGPPRIISPETKYEYLEGEELKAVCVASPPGNPFGGLFWRWLLQPSQVGDQKTGRIIGFGGRGGARMNEYSDVEAYLKTAEHLGSQSADEMSQGMSDLISEDVQPLHYTLNKEKDQLINTLVIPRITRRYHAAKLICETGHPVGSAHQTSIEIFVKYAPANVTISVDGGSVREELVSGRRE--SVVYGQAGEIKTLICRTSPYFKQANIKWITQSSDSGPMKPPRQLFGQTKIMRTPDGKSWLQESRVDVKISEEDDRGYVDCRVWSEGDLRVDARVRMDIIYPPGVPVITGYRSLQP---VKMAHSLEMTCVTSGGNPPPELAWFRDKQQLPNAEEVILLGRQSSLKLKLVPQRDDNGAHYHCAARNAATGNVGIASDAVVLNVVFPPQGVSVNFHGPS--VVPSGQPLVISCQADSSNPVATINWWHFRCGPAHAFSQFKASKTTIEEALYDRRTSERAKCKSEKLEGTEENPVPGTAGGLKAQSRLWLRPTWHYHMDYIECRTENPEYGPPVWHDRLQLNITFAPQ-FLGLQTRKEQVIR-EGTTVNLDLGPYSNPP-----VTSVSWFVEGRPIPPETPPAAARTGVHTSGRLGELLALHKISRTHMANYTVVARNDIAESSAVFFLNVTFPASLAGAAEENVTQTTSDQVTLV-CQATANPSIATQAFTWYRYVPPPGWPQDVNEAEVQLSAPMPCNQSDASPSVDDKY---FIHCQRDDSIHMTSRLSIYRINRDDVGTYVCEIN-NGLGDPVRKNIHFVYHFTPSIVRLPRYTKAAGEQGSTVTLTCLVRTEPTPQIGWLKAGQMIYDSQREGSGALTQKHQTQFLRIRPGLYKSTLTLTNLQKTDFGVYQCQAVNIKGEAMLEVSLSGTSTPDVPLNLRLLNSTANSLRVAWTQGFDGGLTQTFQVRWRLATGSNNMFKYADVAANDNHHAVEYLITGLESGAEYQVSVNSMN 1238
BLAST of NPHS1-5 vs. Ensembl Cavefish
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:ZFIN;Acc:ZDB-GENE-051102-1]) HSP 1 Score: 80.1073 bits (196), Expect = 1.246e-14 Identity = 97/417 (23.26%), Postives = 162/417 (38.85%), Query Frame = 2
Query: 2177 INVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICV----NSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTD--LVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIH--WFK-----DGNDSRILYSTNITSNSSDVIETAIFFANISDA-QFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKI--QWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGES 3379
+NV+F P+ F + ++++V+ + T I V ANPE D C V R F W L I N T G + SN G+ S+ I++D+ Q + V + + V +DL + D N I G++ + ED L Y +++ G Y C + S L + PE+ K RK + V L G + W K D + R L T + + + N+S A + ++C N G+D ++++ + PD P+D + S+ + T+ W GFDGG +Q F++ QWA + + + + P +S Y + L P ++Y+ ++A N GES
Sbjct: 646 LNVLFPPE-FAEDQPSEVQIVEDDTATLPIMVSANPE-DFTCEWLFHGEKVVKDRDHRYH-FPEGWALEIWNVTRRDAGNYRVECSNAEGKRSSIIKLDV--------QYSPTVKMKTDPVFVNVGDTADL----------MCEADANPI------TPGMFSWKWMGEGEVEELGEQSEDGATGLLTLYEVTRAQAGPYQCTADNEIAPPGSVVGQLVVRFAPELQKGPQWRKVASRGDGSTDVDVLCQAEGVPRVTFTWAKNRVPLDFPNPRYL---ERTVREGAIHTSTLTVVNVSAALDYAIFTCTARNSQGEDNLDIQLLSTSYPDPPSDLKLLSVASDSVTLEWMPGFDGGLIQNFRVRYQWA---ESASYLYVDVFPP----RSTVYTVTGLKPVTAYNFSVNAINSMGES 1025 HSP 2 Score: 70.0922 bits (170), Expect = 1.639e-11 Identity = 37/114 (32.46%), Postives = 58/114 (50.88%), Query Frame = 2
Query: 206 QCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIK-HHPLLRRRTHLFVYKKPSFPYIVYNS 544
Q FR P++ T +++C V G VQW + +G R++P PR+ +TGD R +Y L I ++ D+ YECQV K P++ R L V P+ PY +++
Sbjct: 43 QAFRTEPRN-LTVRTGATALLKCEVLRASGSVQWVKDGLLLGPQRSLPGHPRYSMTGDQDRGQYHLQIEDVRLEDDSPYECQVGRSKTSRPIVSRTVWLNVQIPPAEPYFEWDA 155
BLAST of NPHS1-5 vs. Ensembl Cavefish
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 66.2402 bits (160), Expect = 2.008e-10 Identity = 89/396 (22.47%), Postives = 155/396 (39.14%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCS--LSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGN--FTISWEFQRNSQMMILEDDI 1387
F + PQD + V + C++ HG V W + +G R + PR+ + GD S E+ L I ++ D+ +ECQ + R L V P P I I+ + ++L C + + P + WI + + L N S D +S S +PS ++ + C SN+A+ P+ T V I Q P + + + V + + ++ CS +PP + ++KW+ +++ S T E ++ + + TN L + N+S R +++YF GP +A+ Q L V G F C + GN TI W +R S +++ ++I
Sbjct: 2 FSQQPQDLV-VVAGQPVTLPCSIPGYHGVVLWIKDGLALGVGRDLSGYPRYTVVGDHSSGEHHLRIQRVELMDDAVFECQA---IQAAMRSRPARLTVLVPPEDPVI-------DGGPIVSLRAGDPLNLTCHADNAKPAASIIWIRNGEVL---NGAMYSKTLLRDGRRESTVSTLYISPSNIETGQKIICRASNKAI-----PNGKETSVTIDIQHLP-LVNLSVEPQPVLEGNLVKFHCSAKANPP-----VTQYKWAKGGT--------VIKEVSGDTYE---VIVDHSFFTEAVSCEVTNPLGST--------NIS---------RNVDVYF--GPRMAAEPQS----LQVDLGSDAVFNC---AWTGNPSLTIVW-MKRGSGVVLSNENI 334
BLAST of NPHS1-5 vs. Ensembl Cavefish
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:HGNC Symbol;Acc:HGNC:23204]) HSP 1 Score: 65.855 bits (159), Expect = 2.196e-10 Identity = 89/396 (22.47%), Postives = 155/396 (39.14%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCS--LSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGN--FTISWEFQRNSQMMILEDDI 1387
F + PQD + V + C++ HG V W + +G R + PR+ + GD S E+ L I ++ D+ +ECQ + R L V P P I I+ + ++L C + + P + WI + + L N S D +S S +PS ++ + C SN+A+ P+ T V I Q P + + + V + + ++ CS +PP + ++KW+ +++ S T E ++ + + TN L + N+S R +++YF GP +A+ Q L V G F C + GN TI W +R S +++ ++I
Sbjct: 2 FSQQPQDLV-VVAGQPVTLPCSIPGYHGVVLWIKDGLALGVGRDLSGYPRYTVVGDHSSGEHHLRIQRVELMDDAVFECQA---IQAAMRSRPARLTVLVPPEDPVI-------DGGPIVSLRAGDPLNLTCHADNAKPAASIIWIRNGEVL---NGAMYSKTLLRDGRRESTVSTLYISPSNIETGQKIICRASNKAI-----PNGKETSVTIDIQHLP-LVNLSVEPQPVLEGNLVKFHCSAKANPP-----VTQYKWAKGGT--------VIKEVSGDTYE---VIVDHSFFTEAVSCEVTNPLGST--------NIS---------RNVDVYF--GPRMAAEPQS----LQVDLGSDAVFNC---AWTGNPSLTIVW-MKRGSGVVLSNENI 334
BLAST of NPHS1-5 vs. Ensembl Cavefish
Match: kirrel3a (kirre like nephrin family adhesion molecule 3a [Source:ZFIN;Acc:ZDB-GENE-091117-44]) HSP 1 Score: 63.5438 bits (153), Expect = 1.329e-9 Identity = 53/220 (24.09%), Postives = 86/220 (39.09%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRP 871
F + P D + V + C++ HG V W + +G +R + PR+ + GD S+ EY L I + D+G +ECQ + R L V P P I+ I+ + ++L C + + P + WI + + L N S D +S S + S ++ + C SN+A P+ V I Q P
Sbjct: 31 FVQQPHDVV-VVAGQPVTLPCSITGFHGMVLWDG--LALGVNRDLSGYPRYDVIGDHSKGEYHLLIQRTELGDDGSFECQG---IQAGIRSRPARLTVLVPPDDPVII-------GAPIVSLRAGDPLNLTCHADNAKPAASIIWIRNGEVL---NGAMYSKTLLRDGQRESTVSTLYISASNIESGQRITCRASNKA-----APNGKEATVTIDIQHLP 229
BLAST of NPHS1-5 vs. Ensembl Cavefish
Match: kirrel3l (kirre like nephrin family adhesion molecule 3, like [Source:ZFIN;Acc:ZDB-GENE-070831-1]) HSP 1 Score: 58.5362 bits (140), Expect = 4.383e-8 Identity = 49/220 (22.27%), Postives = 84/220 (38.18%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIH-LECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKR 868
F + PQD+ + +V + C + G VQW + +G +R +P R+ + GD + E+ L I + +D+ YECQ L R L V PS P + + ++ + H L C + P +W D + + + + E D ++ S P C + N A P+ +T V I Q++
Sbjct: 18 FSQQPQDQV-VVAGQSVTLPCVIVGYRGMVQWTKDGLALGGERDLPGWTRYSLLGDPASGEHSLVIESSELADDAVYECQA---TQAGLRSHRARLTVLVPPSDPVV--------DGGPVVRLKAHTPHNLTCRATGAKPAAEITWYRDGEVMHTAIYSKSLME---DGKRETAVSLLPMTPEDRDSGRTYTCRVLNPA-----APAGRQTSVTISVQRK 217
BLAST of NPHS1-5 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008186.1 (pep scaffold:Pmarinus_7.0:GL476501:83598:168579:1 gene:ENSPMAG00000007342.1 transcript:ENSPMAT00000008186.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 55.8398 bits (133), Expect = 7.711e-8 Identity = 52/232 (22.41%), Postives = 96/232 (41.38%), Query Frame = 2
Query: 2714 LVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDG---NDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGK-LQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIK-----DLNPNSSYSIKISASNIFGESE 3382
L+ + + D G Y+C V+ +G+ +K +L + P +V Q+ + ++ C P I W K+ + R+ T ++ D + + + G ++C N G+D +++ PD P + V+ + T+ W GFDG + F I++ R +W + RT+ +S I DL+P + Y ++ A N G SE
Sbjct: 585 LLLRQVQEEDGGFYLCQVS-NGVGTDISKSMLLNVKIPALVTSQANVTLATRG-QHSNLQCSARGERPIIIRWEKEETLIDPERMFRYVVYTQDTGDELLSTLQILPTVRGDSGFFTCHAINSYGEDRGLIQLTVQEPPDPP-EVEVREVNARTITLRWTSGFDGNSPITAFDIEYKN---RSDTWEQK-----QRTKDISSTINQATIIDLHPATHYVFRVYAKNRIGASE 805
BLAST of NPHS1-5 vs. Ensembl Sea Lamprey
Match: nfasca (neurofascin homolog (chicken) a [Source:ZFIN;Acc:ZDB-GENE-080229-6]) HSP 1 Score: 54.299 bits (129), Expect = 2.023e-7 Identity = 56/238 (23.53%), Postives = 93/238 (39.08%), Query Frame = 2
Query: 2705 STDLVFYYISQGDNGRYVCNV--------TVDGLLVASTKYILSILMQPEIVKYQ--SIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGG-KLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESES 3385
S LV + +GD G Y C+V ++ LL+ K I+ P ++ Q + F C A + S+S +I WF DG Y S ++ + + N+++ G Y C S E + PD P D + + + + W G + ++ F +++ D+ WH P +R S L+ L+P SY ++ A N G SE
Sbjct: 454 SDTLVVPRVQKGDAGVYTCHVQNYLNKNQSLARLLI---KDPTRIVQPPSELRAQRFGVATFECHATHDPSLSL--------NISWFHDG------YPLP-ESPRHELEGSGLTIVNVTEEDVGHYKCRASTELDHAEAEARLTVIDRPDSPMDLELTNRQDLSVRLTWNPGSENNDPIKEFVVEYEEDWFEPGVWHRLSSFPGSRN---SVLLDSLSPYVSYRFRVVAVNSVGPSEP 670
BLAST of NPHS1-5 vs. Ensembl Nematostella
Match: EDO26116 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7TCU5]) HSP 1 Score: 53.9138 bits (128), Expect = 1.556e-7 Identity = 42/147 (28.57%), Postives = 69/147 (46.94%), Query Frame = 2
Query: 2996 VIETAIFFAN-ISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFW-QQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSL 3430
V +T+++ ++ ++ ++ G Y+C +NE G D TV+V P P TV +IE + + W DGG I DYK + P +I DL N+ Y++K++A N+ G E+ T + +TK SL
Sbjct: 52 VSQTSVYNSDSVTRSEAGTYTCTATNEMGSDTTTVQVTVNFAPGPPI-VTVSNIEASSIKVNWAPPSSDGGSPVI-------DYKVEV---DPAKPPREGVAGDQVVISDLTKNTEYTVKVTARNVVGYGEAGTKTIRTKKQGPPSL 187
BLAST of NPHS1-5 vs. Ensembl Medaka
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:NCBI gene;Acc:101155460]) HSP 1 Score: 83.5741 bits (205), Expect = 9.574e-16 Identity = 160/774 (20.67%), Postives = 289/774 (37.34%), Query Frame = 2
Query: 1145 NQSIANVSVFIMINFTP----RKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEF---QRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV---GEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIV----KSKFSMRAISTRIIIERKHDQCRIY-CQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKW----NLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDII-ACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGC--DALSNCSVSCLFTLSGPADIHWFKDG---NDSRILYSTNITSNSSDVIETAIFFANISDAQ-FGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLK--PSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESE 3382
NQ+ + F ++ +P RKL+++F L S L +G+ C + I W + N+ + I E+ + T S+V + + + +L C G + V Y P+ + + + D L G ++ + C GNP P L +G + ++ M A ++ D Y C A + + +K L+ + I V+ G+ T+ C SNP I W + + F++ +++ V N + + + SQ +H VIC+ YN ++ +NV+ P+ F + + +I++ + E T + V ANPE + CV TL+K + W +L I N+T G I N G + T I +D++ AC +++ V V+L +++ + + + + ++ W ET +++S+ L +++ +G Y C L S L + +PE+ K RK D + + C D W K G + + Y S T + N+S + +SC N G+D +++V PD P+ S T+ W GF+GG Q F+I+ Y+ D S +++ P + T ++ + L P + Y+ I+A N GES+
Sbjct: 300 NQAELSCESFNAVSLSPLSITRKLDVFFDPEELTLSGS------LEAIEGQEVVLSCFAASSNPPVDIRWWLGYKELNNTVFIREEG-----DNGGMTTVSNVTYRFSREDNGLMLNCEAFNKGTRFSKIKDAKISVYYPPQKVWLDAPA--QDVPLNAGTKVTLICHSTGGNPTPTLTWLKNGKAVAHAPKQTSSDMAAARELHLVLTPSDNMATYRCDATNNAKKTISAHAKL------LVYFTAVSMKITAHQEVLRQGEVLTLECLAESSNPTANIFWSVGAVR-----FQKAEQTHSKDKSGAVTVNSTLLLNLTSQ------------HHKQNVICQAYNPVL-EEGANTYYRLNVLHPPE-FSSAQPTEIQVEEDEMATIPLLVSANPE-GVSCVWLHRRETLVKG-NNLHHYWLEDGSLEIRNATRRDAGLYTIKCENEEGVSQTAITLDVLYACDVTAEKDPVFVNLGGTADLICVADANPIISDMFSWKWLGEEEVEMGEET------------------------QEDESSLLTIRNVTRAHSGLYQCTANNGIGLSGSVNMQLVVQFKPELQKGPQWRKVASRGDGSTTAELVCRAEGIPRIDFSWEKKGVFLDLANPRYEEKTVREGSFHTST-LRVVNVSAVMDYAVFSCYARNSLGEDKVDIQLVSTNHPDPPSMFRHVSSTHDSVTLEWIPGFNGGLQQKFRIR----YQWDQSVSFQYVDVFPPDAT---TFTVTGLQPVTVYNFSINALNAIGESD 1001 HSP 2 Score: 53.9138 bits (128), Expect = 1.284e-6 Identity = 40/151 (26.49%), Postives = 64/151 (42.38%), Query Frame = 2
Query: 92 AWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQV-EPIKHHPLLRRRTHLFVYKKPSFPYIVYN 541
AW++ + F LC + GA+ Q+ + P ++ R+ T V++C V G VQW + +G R++P PR+ + G R +Y L I Q D+ YECQ + ++ L V PS PY +
Sbjct: 6 AWIVSPSTLPF--LCLIWGAQA-QQAFRIEPRNLTVRMGA---------------TAVLKCEVLRASGTVQWVKDGLLLGPQRSLPGFPRYSMIG--KRGQYHLRIENAQLEDDTFYECQAGQSDTSEGIISNAVWLDVLIPPSNPYFTMD 136
BLAST of NPHS1-5 vs. Ensembl Medaka
Match: nphs1 (NPHS1 adhesion molecule, nephrin [Source:NCBI gene;Acc:101155460]) HSP 1 Score: 82.0333 bits (201), Expect = 3.601e-15 Identity = 164/770 (21.30%), Postives = 290/770 (37.66%), Query Frame = 2
Query: 1145 NQSIANVSVFIMINFTP----RKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEF---QRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV---GEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIV----KSKFSMRAISTRIIIERKHDQCRIY-CQAHSQHFPHMIVKSKYINLSLALLPREVIVKY-IHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQ-TVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDII-ACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGC--DALSNCSVSCLFTLSGPADIHWFKDG---NDSRILYSTNITSNSSDVIETAIFFANISDAQ-FGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESE 3382
NQ+ + F ++ +P RKL+++F L S L +G+ C + I W + N+ + I E+ + T S+V + + + +L C G + V Y P+ + + + D L G ++ + C GNP P L +G + ++ M A ++ D Y C A + K I+ LL V I V+ G+ T+ C SNP I W + + VT K V N + + + SQ +H VIC+ YN ++ +NV+ P+ F + + +I++ + E T + V ANPE + CV TL+K P S L I N+T G I N G + T I +D++ AC +++ V V+L +++ + + + ++ ++S + ++ +E + + +++S+ L +++ +G Y C L S L + +PE+ K RK D + + C D W K G + + Y S T + N+S + +SC N G+D +++V PD P+ S T+ W GF+GG Q F+I++ D + S+ + P + T ++ + L P + Y+ I+A N GES+
Sbjct: 307 NQAELSCESFNAVSLSPLSITRKLDVFFDPEELTLSGS------LEAIEGQEVVLSCFAASSNPPVDIRWWLGYKELNNTVFIREEG-----DNGGMTTVSNVTYRFSREDNGLMLNCEAFNKGTRFSKIKDAKISVYYPPQKVWLDAPA--QDVPLNAGTKVTLICHSTGGNPTPTLTWLKNGKAVAHAPKQTSSDMAAARELHLVLTPSDNMATYRCDATNN-------AKKTISAHAKLLVYFTAVSMKITAHQEVLRQGEVLTLECLAESSNPTANIFWSVGAVRFGVTLSTK--------HQSGAVTVNSTLLLNLTSQ------------HHKQNVICQAYNPVL-EEGANTYYRLNVLHPPE-FSSAQPTEIQVEEDEMATIPLLVSANPE-GVSCVWLHRRETLVKG-DPDGS---LEIRNATRRDAGLYTIKCENEEGVSQTAITLDVLYACDVTAEKDPVFVNLGGTADLICVADANPI----ISDMFSWKWLEGERVE--------------------MGEETQEDESSLLTIRNVTRAHSGLYQCTANNGIGLSGSVNMQLVVQFKPELQKGPQWRKVASRGDGSTTAELVCRAEGIPRIDFSWEKKGVFLDLANPRYEEKTVREGSFHTST-LRVVNVSAVMDYAVFSCYARNSLGEDKVDIQLVSTNHPDPPSMFRHVSSTHDSVTLEWIPGFNGGLQQKFRIRYQWD--QSVSFQYVDVFPPDAT---TFTVTGLQPVTVYNFSINALNAIGESD 999
BLAST of NPHS1-5 vs. Ensembl Medaka
Match: kirrel1a (kin of IRRE-like protein 1 [Source:NCBI gene;Acc:101168819]) HSP 1 Score: 66.6254 bits (161), Expect = 1.519e-10 Identity = 66/269 (24.54%), Postives = 104/269 (38.66%), Query Frame = 2
Query: 200 RLQCFRETPQDKYESIV-NDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMP-KIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESI 1000
R F + P D +S+V V++ C V N G VQW + +G + A PR+R+ +Y L I SD+ YECQ L RR L V P P I ++L++ + +L C+S+ P T W+ D +E + E +D + SH +P C+ SN A+ PS T V + P + I V DR + +PP ++ ++W+ +
Sbjct: 14 RTARFSQEPAD--QSVVRGQRVILSCVVFNYSGIVQWTKDGLALGIGEDLRAWPRYRVLRVQESGQYNLEILSADLSDDAVYECQA---PDAALRSRRAKLTVLIPPDDPVI------DGGPEVLLN-AGESYNLSCVSRGAKPPSTIEWLKDGLPVEG---AVSITEVLADRKRVTTQSHLPIHPVDTDTGRNYSCVTSNLAV-----PSGKSTTVTLNVHHPPVVTLSIEPRSVLEGDRVTFTCQAHANPP-----IMGYRWAKGGV 257
BLAST of NPHS1-5 vs. Ensembl Medaka
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:NCBI gene;Acc:101170290]) HSP 1 Score: 63.5438 bits (153), Expect = 1.189e-9 Identity = 59/267 (22.10%), Postives = 102/267 (38.20%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESR-SDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRI 1009
F + PQD + V + C++ HG V W + +G R + PR+ + GD E+ L I ++ D+ +ECQ + R L V P P I ++ + ++L C + + P + W+ + + L S E+ D +S S +PS ++ + C SN+A+ P+ V I Q P + + + V + +R CS + P L W IK +
Sbjct: 20 FSQQPQDLV-VVAGQPVTLPCSIPGYHGIVLWIKDGLALGVGRDLSGYPRYTVIGDHGSGEHHLRIQRVELMDDAVFECQA---VQAAMRSRPARLTVLVPPDDPVI-------GGGPVVSLRAGDPLNLTCHADNAKPAASIIWMRNGEVLNGAMYSKVSIEALVRDGRRESTVSTLYLSPSNIETGQQIICRASNKAV-----PNGKEASVTIDIQHLP-LVNLSVEPQAVLEGSLVRFQCS-AKANPPVTLYRWAKGGSIIKEV 268
BLAST of NPHS1-5 vs. Ensembl Medaka
Match: KIRREL3 (kirre like nephrin family adhesion molecule 3 [Source:NCBI gene;Acc:101170290]) HSP 1 Score: 63.5438 bits (153), Expect = 1.338e-9 Identity = 59/266 (22.18%), Postives = 101/266 (37.97%), Query Frame = 2
Query: 212 FRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRI 1009
F + PQD + V + C++ HG V W + +G R + PR+ + GD E+ L I ++ D+ +ECQ + R L V P P I ++ + ++L C + + P + W+ + + L N S D +S S +PS ++ + C SN+A+ P+ V I Q P + + + V + +R CS + P L W IK +
Sbjct: 48 FSQQPQDLV-VVAGQPVTLPCSIPGYHGIVLWIKDGLALGVGRDLSGYPRYTVIGDHGSGEHHLRIQRVELMDDAVFECQA---VQAAMRSRPARLTVLVPPDDPVI-------GGGPVVSLRAGDPLNLTCHADNAKPAASIIWMRNGEVL---NGAMYSKALVRDGRRESTVSTLYLSPSNIETGQQIICRASNKAV-----PNGKEASVTIDIQHLP-LVNLSVEPQAVLEGSLVRFQCS-AKANPPVTLYRWAKGGSIIKEV 292
BLAST of NPHS1-5 vs. Planmine SMEST
Match: SMESG000003549.1 (SMESG000003549.1) HSP 1 Score: 2474.12 bits (6411), Expect = 0.000e+0 Identity = 1217/1224 (99.43%), Postives = 1221/1224 (99.75%), Query Frame = 2
Query: 77 MPSRLAWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHVGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQYLKVLAYXXXXXXXXXXXXXXXXXXXKVNSSMKDRRSLHHKSMTHFISLNNRLYSSMIEDNKDISMIFTNRISSSPENSRLNDSGRDSLVNISDNA 3748
MPSRLAWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLC+SVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQ+MILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHVGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRA+STRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNS VICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDI ACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQ LSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQYLKVLAYTLAICSLAILLIIIITGLLKVNSSMKDRRSLHHKSMTHFISLNNRLYSSMIEDNKDISMIFTNRISSSP+NSRLNDSGRDSLVNISDNA
Sbjct: 1 MPSRLAWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCESVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQIMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHVGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAVSTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSAVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDITACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQILSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQYLKVLAYTLAICSLAILLIIIITGLLKVNSSMKDRRSLHHKSMTHFISLNNRLYSSMIEDNKDISMIFTNRISSSPDNSRLNDSGRDSLVNISDNA 1224
BLAST of NPHS1-5 vs. Planmine SMEST
Match: SMESG000003549.1 (SMESG000003549.1) HSP 1 Score: 2321.2 bits (6014), Expect = 0.000e+0 Identity = 1159/1224 (94.69%), Postives = 1169/1224 (95.51%), Query Frame = 2
Query: 77 MPSRLAWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQMMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHVGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAISTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTSWHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQYLKVLAYXXXXXXXXXXXXXXXXXXXKVNSSMKDRRSLHHKSMTHFISLNNRLYSSMIEDNKDISMIFTNRISSSPENSRLNDSGRDSLVNISDNA 3748
MPSRLAWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLC+SVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQ+MILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHVGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRA+STRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNS VICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDI ACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVE + P + G G + +K + +R TS LSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQYLKVLAYTLAICSLAILLIIIITGLLKVNSSMKDRRSLHHKSMTHFISLNNRLYSSMIEDNKDISMIFTNRISSSP+NSRLNDSGRDSLVNISDNA
Sbjct: 1 MPSRLAWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPIKHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLECISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPSSYRTFVQIRFQKRPGMPKIPQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFSLRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCESVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDFSILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQIMILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHVGEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAVSTRIIIERKHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQFKEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSAVICKVYNYHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPEEDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDITACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWSLRRMDHNFIETILGCNRGLYFNNATSRYLKLFVKCSKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYILSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRILYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVESNQLKQP-----------------LLRYSGSKGLMVGSYKYLNRFNGQRITSI-------------LSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQYLKVLAYTLAICSLAILLIIIITGLLKVNSSMKDRRSLHHKSMTHFISLNNRLYSSMIEDNKDISMIFTNRISSSPDNSRLNDSGRDSLVNISDNA 1194
BLAST of NPHS1-5 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 355.14 bits (910), Expect = 3.048e-101 Identity = 304/1158 (26.25%), Postives = 515/1158 (44.47%), Query Frame = 2
Query: 146 GAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPI-KHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLE--CISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPS--SYRTFVQIRFQKRPGMPKI----PQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFS-------LRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDF---SILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQM---MILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV-GEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAIST----RIIIER--KHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQF--KEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYN--YHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPE-EDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWS--LRRMDHNFIET-------ILGCNRGLYFNNATSRYLKLFVKC----SKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYI-LSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRI----LYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTS-WHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQ 3460
GAK I QS +C+ E+I R+QCF+E P+ YE +RC V NQ+G+VQWRA V +G+DR IP +P +R+ G + E+FL I + D G YECQV+P+ + HPLLRR+T L V +P P I Y + ++I+ K+ + E C+S +G P + W ++N ++ DN N + + S S SK +D +++C +SNRA + P S T V I PG P+I P D++ ++C SP G P L W+W ++ + F QY +T N++V+ L +P + + H L C + S + +V + I P K+ I ++G L + + KD +++ + GE C T YY TI W F N + I + WKE+++ F + S++++K+N S L C + V + + L + Y P I V + + ++ C+ GNP P+L + D + ++S F + + +III ++ + +CQA S + S + L + P + +K + VE+G+ + CT SNP V+ W+L C + + + ++ +C + + SN S + S + PSW H V C+V+N Y ++++ I INV F P+ F + +G+ +T ++ IANP +D +S ++ SK L I N+ V +V N G + I ++I + + + + CS S+ N ++++ W+ + +N I + +GCN+ + K F KC E ++ L+ Y + + D G Y C V +G+ K I L+ P+I+ K + S+ C + WF ++ + Y NIT + + N+ G Y C+ N G D +EV + PD P + V + + I W QGFDGG +Q F+++W K TS + E + + +LS++I L +++Y I I++ N S+ TS F +K +++S+ + + Q
Sbjct: 21 GAK-IKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQ-----DNENSALLEELNYS-RSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDES-FRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNV--GKPVKLNHNFEMTCSTIGGNPYPELQWFRDS-KPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSL--SGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQ-FEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRC-VVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKE--KSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRN----SKHGTSQF-SKTIMTNTMSADNEKENNQ 1156
BLAST of NPHS1-5 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 355.14 bits (910), Expect = 3.387e-101 Identity = 304/1158 (26.25%), Postives = 515/1158 (44.47%), Query Frame = 2
Query: 146 GAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPI-KHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLE--CISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPS--SYRTFVQIRFQKRPGMPKI----PQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFS-------LRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDF---SILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQM---MILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV-GEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAIST----RIIIER--KHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQF--KEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYN--YHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPE-EDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWS--LRRMDHNFIET-------ILGCNRGLYFNNATSRYLKLFVKC----SKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYI-LSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRI----LYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTS-WHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQ 3460
GAK I QS +C+ E+I R+QCF+E P+ YE +RC V NQ+G+VQWRA V +G+DR IP +P +R+ G + E+FL I + D G YECQV+P+ + HPLLRR+T L V +P P I Y + ++I+ K+ + E C+S +G P + W ++N ++ DN N + + S S SK +D +++C +SNRA + P S T V I PG P+I P D++ ++C SP G P L W+W ++ + F QY +T N++V+ L +P + + H L C + S + +V + I P K+ I ++G L + + KD +++ + GE C T YY TI W F N + I + WKE+++ F + S++++K+N S L C + V + + L + Y P I V + + ++ C+ GNP P+L + D + ++S F + + +III ++ + +CQA S + S + L + P + +K + VE+G+ + CT SNP V+ W+L C + + + ++ +C + + SN S + S + PSW H V C+V+N Y ++++ I INV F P+ F + +G+ +T ++ IANP +D +S ++ SK L I N+ V +V N G + I ++I + + + + CS S+ N ++++ W+ + +N I + +GCN+ + K F KC E ++ L+ Y + + D G Y C V +G+ K I L+ P+I+ K + S+ C + WF ++ + Y NIT + + N+ G Y C+ N G D +EV + PD P + V + + I W QGFDGG +Q F+++W K TS + E + + +LS++I L +++Y I I++ N S+ TS F +K +++S+ + + Q
Sbjct: 40 GAK-IKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQ-----DNENSALLEELNYS-RSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDES-FRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNV--GKPVKLNHNFEMTCSTIGGNPYPELQWFRDS-KPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSL--SGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQ-FEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRC-VVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKE--KSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRN----SKHGTSQF-SKTIMTNTMSADNEKENNQ 1175
BLAST of NPHS1-5 vs. Planmine SMEST
Match: SMESG000028689.1 (SMESG000028689.1) HSP 1 Score: 348.977 bits (894), Expect = 4.236e-99 Identity = 308/1176 (26.19%), Postives = 518/1176 (44.05%), Query Frame = 2
Query: 146 GAKVIVQSSQCSPEDIEKRLQCFRETPQDKYESIVNDTVVMRCAVDNQHGRVQWRAKEVTMGFDRAIPAKPRFRITGDASRSEYFLTIYPIQFSDNGDYECQVEPI-KHHPLLRRRTHLFVYKKPSFPYIVYNSKNVSNMDILIDQQKKCIHLE--CISKHGFPIPTYSWIVDNKFLESFNCKDNSNESRSDPSYKSPPSHFKFNPSKLQDQLLVECIISNRALALDSGPS--SYRTFVQIRFQKRPGMPKI----PQDKVTVNDRDSLRVSCSLSPPGLPKALLEWKWSNESIKRIPLAYFS-------LRQYSVKTTENNRIVNVLNLPVVGIEMHATNLLCQSVYLNQSIANVSVFIMINFTPRKLEIYFANGPLLASSEQKDF---SILPVKDGEIYFFKCQTSEYYGNFTISWEFQRNSQM---MILEDDIHWKENKNFFTQTSSVELKVNSAMHSSILICHV-GEVDKVSASIHLEVLYFPRVINISSSIVHPDSYLTVGDEIQVNCTVGDGNPIPDLAMYIDGIQIVKSKFSMRAIST----RIIIER--KHDQCRIYCQAHSQHFPHMIVKSKYINLSLALLPREVIVKYIHPKNGVVESGQRQTISCTVTGSNPEPVISWRLKLCQTVTKQF--KEIKKSCQLQDMKTVYSNFSFINHKESQLTSVLKWQPSWIYHNSTVICKVYN--YHFPQKIIQNSIDINVVFRPKIFFNHREKKIKLVKGEGKTFVIDVIANPE-EDLICVNSSVTITLIKRIKPFYSKWNLSIDNSTDVKRGRLLIVASNTMGRTSTFIQVDIIACHSDVQQETVHVSLSSITNIVTCSHPSDLDNRNVTFLWS--LRRMDHNFIET-------ILGCNRGL-----YFNNATSRY-------------LKLFVKC----SKEDSTDLVFYYISQGDNGRYVCNVTVDGLLVASTKYI-LSILMQPEIVKYQSIRKFGCDALSNCSVSCLFTLSGPADIHWFKDGNDSRI----LYSTNITSNSSDVIETAIFFANISDAQFGKYSCEISNEAGKDIWTVEVVEPTLPDKPTDCTVKSIETTFATIFWQQGFDGGKLQIFKIQWATDYKRDTS-WHSEFLKPSNRTQSLSYLIKDLNPNSSYSIKISASNIFGESESCTSSFQTKAFTMSSLSSTDRIIDQQ 3460
GAK I QS +C+ E+I R+QCF+E P+ YE +RC V NQ+G+VQWRA V +G+DR IP +P +R+ G + E+FL I + D G YECQV+P+ + HPLLRR+T L V +P P I Y + ++I+ K+ + E C+S +G P + W ++N ++ DN N + + S S SK +D +++C +SNRA + P S T V I PG P+I P D++ ++C SP G P L W+W ++ + F QY +T N++V+ L +P + + H L C + S + +V + I P K+ I ++G L + + KD +++ + GE C T YY TI W F N + I + WKE+++ F + S++++K+N S L C + V + + L + Y P I V + + ++ C+ GNP P+L + D + ++S F + + +III ++ + +CQA S + S + L + P + +K + VE+G+ + CT SNP V+ W+L C + + + ++ +C + + SN S + S + PSW H V C+V+N Y ++++ I INV F P+ F + +G+ +T ++ IANP +D +S ++ SK L I N+ V +V N G + I ++I + + + + CS S+ N ++++ W+ + +N I + +GCN+ L Y N Y K F KC E ++ L+ Y + + D G Y C V +G+ K I L+ P+I+ K + S+ C + WF ++ + Y NIT + + N+ G Y C+ N G D +EV + PD P + V + + I W QGFDGG +Q F+++W K TS + E + + +LS++I L +++Y I I++ N S+ TS F +K +++S+ + + Q
Sbjct: 45 GAK-IKQSIECTNEEIVNRIQCFQEIPKPVYEVAQGSAATLRCIVRNQYGKVQWRAVPVLLGYDRNIPGRPTYRVVGSVVKGEHFLEISNVTVKDAGIYECQVQPVGQEHPLLRRKTKLVVLVRPDQPQIFYKGFQPNGDQVIINSIKQDVRTEFTCVSDNGQPKVKFQWFLNNNLIQ-----DNENSALLEELNYS-RSRLSLKTSKFKDGDVIKCRVSNRATEVHQDPERRSLSTEVFIVIHTPPGQPQIVDMNPHKTTPYVVDDNIIMTCESSPSGKPAGSLLWRWKYPKVRELYNNRFENYTTKDISPQYYQQTISKNKMVSTLTIPALTSKEHGAQLFCVATNPVISYQSTAVKLYILHIPNKVVISVSDGSLSSRTIGKDGLNENVIYGEAGEEKTITCTTDPYYNEATIKWVFSPNPAINNDFISPVEKIWKEDES-FRRNSTIKIKINKQFDQSFLDCQAWSGIKSVQSRVKLHITYSPEKPTIQGYNV--GKPVKLNHNFEMTCSTIGGNPYPELQWFRDS-KPIQSSFIAQQVGNQKTLKIIITPSINDNKVKYFCQAKSISLKSSPLNSDEVTLIVLFPPMTIGLKSL--SGAEVENGKLLKLQCTAGESNPPAVVRWKLNRCGQMMSELGRRPVQTTCNEELLSASSSNTINGKFGGSLIQSYFEIYPSWKDHLDYVDCEVFNPDYGEEHQVLRKRIQINVTFSPQ-FEGIINYNFYIEEGQSQTLNLEPIANPPIKDYTWFRNSTKLSRKNVHSISTSKAKLIIRNAKPVDIDNYTLVVVNDRGESQITIYLNITYQPIIIGDKFERKEIIRQNAELECSVQSNPANPHMSYKWTKLVPGKGYNDISSSDLVPAFTIGCNQSLPFSKEYKNEKPKPYSKAEWKGTPEKSNFKYFSKCMVKSQFEMTSLLIIYDVDRNDVGLYRC-VVNNGIRGDVVKTIELTKEFTPQILSLPKFSKAAGNLGEEVSLFCYIRSHPSPKMTWFFGRYNTSVSANSKYRINITRFRTGFYVAQLKIVNLQRTDHGSYVCKAENGKGSDSRKIEVSGTSTPDVPLEIQVVNKSQSQIEISWIQGFDGGSIQTFQVRWKE--KSPTSLYRYEDVASDDTKITLSFIITGLKSDTAYLISINSRN----SKHGTSQF-SKTIMTNTMSADNEKENNQ 1198 The following BLAST results are available for this feature:
BLAST of NPHS1-5 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of NPHS1-5 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of NPHS1-5 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of NPHS1-5 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of NPHS1-5 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of NPHS1-5 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of NPHS1-5 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of NPHS1-5 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of NPHS1-5 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of NPHS1-5 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of NPHS1-5 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of NPHS1-5 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of NPHS1-5 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of NPHS1-5 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30002472 ID=SMED30002472|Name=NPHS1-5|organism=Schmidtea mediterranea sexual|type=transcript|length=3793bpback to top protein sequence of SMED30002472-orf-1 >SMED30002472-orf-1 ID=SMED30002472-orf-1|Name=SMED30002472-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1225bp MPSRLAWLIQWWLISFTNLCRLGGAKVIVQSSQCSPEDIEKRLQCFRETPback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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