Peptidase inhibitor 16

Overview
NamePeptidase inhibitor 16
Smed IDSMED30002471
Length (bp)1929
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Peptidase inhibitor 16 (SMED30002471) t-SNE clustered cells

Violin plots show distribution of expression levels for Peptidase inhibitor 16 (SMED30002471) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002471

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30002471h1SMcG0004643 SmedASXL_013567SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
parapharyngeal regionSMED30002471h1SMcG0004643 SmedASXL_013567SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
gutSMED30002471h1SMcG0004643 dd_Smed_v4_2021_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30002471h1SMcG0004643 dd_Smed_v6_2021_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 95.5153 bits (236), Expect = 3.279e-20
Identity = 61/190 (32.11%), Postives = 92/190 (48.42%), Query Frame = 2 
Query:   95 ILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDG-YGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSG---QKDQPYEIGKPCSECSSSYQFCRDDLCVP 646
            +  LT  E++  ++LHN  R  V P ASDM  + ++E +      Y  QC + H K  G  GEN    +D   +V   +  W  ER ++N+SA     CS             CGHY  +V +  + +GCG  FC+ +Q  G+  T+  +  C Y      +  +PY+ G PCS+C S Y  C++ LC P
Sbjct:   25 VGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSA---ATCSPGQ---------MCGHYTQVVWAKTERIGCGSHFCEKLQ--GVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYH-CKNSLCEP 199
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 95.5153 bits (236), Expect = 3.279e-20
Identity = 61/190 (32.11%), Postives = 92/190 (48.42%), Query Frame = 2 
Query:   95 ILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDG-YGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSG---QKDQPYEIGKPCSECSSSYQFCRDDLCVP 646
            +  LT  E++  ++LHN  R  V P ASDM  + ++E +      Y  QC + H K  G  GEN    +D   +V   +  W  ER ++N+SA     CS             CGHY  +V +  + +GCG  FC+ +Q  G+  T+  +  C Y      +  +PY+ G PCS+C S Y  C++ LC P
Sbjct:   25 VGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSA---ATCSPGQ---------MCGHYTQVVWAKTERIGCGSHFCEKLQ--GVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYH-CKNSLCEP 199
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Human
Match: PI16 (peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGNC:21245])

HSP 1 Score: 95.5153 bits (236), Expect = 3.279e-20
Identity = 61/190 (32.11%), Postives = 92/190 (48.42%), Query Frame = 2 
Query:   95 ILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDG-YGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSG---QKDQPYEIGKPCSECSSSYQFCRDDLCVP 646
            +  LT  E++  ++LHN  R  V P ASDM  + ++E +      Y  QC + H K  G  GEN    +D   +V   +  W  ER ++N+SA     CS             CGHY  +V +  + +GCG  FC+ +Q  G+  T+  +  C Y      +  +PY+ G PCS+C S Y  C++ LC P
Sbjct:   25 VGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSA---ATCSPGQ---------MCGHYTQVVWAKTERIGCGSHFCEKLQ--GVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYH-CKNSLCEP 199
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Human
Match: R3HDML (R3H domain containing like [Source:HGNC Symbol;Acc:HGNC:16249])

HSP 1 Score: 87.8113 bits (216), Expect = 8.742e-19
Identity = 53/186 (28.49%), Postives = 93/186 (50.00%), Query Frame = 2 
Query:  113 RERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHE-VQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFY---NSGQKDQPYEIGKPCSECSSSYQ-FCRDDLC 640
            R+    LD HNH+R SVYPPA++M+ + +++ + ++   +  QC + H   + +   G+N  + S    +V + + SW +E+ ++   A  +      N   P+  +   C HY  MV ++   LGC    C +I   G ++  +    C Y    +   + PY++GKPCS C  SYQ  C  ++C
Sbjct:   61 RDMNALLDYHNHIRASVYPPAANMEYMVWDKRLARAAEAWATQCIWAHGPSQLMRYVGQNLSIHSGQYRSVVDLMKSWSEEKWHYLFPAPRD-----CNPHCPWRCDGPTCSHYTQMVWASSNRLGCAIHTCSSISVWGNTWHRAAYLVCNYAIKGNWIGESPYKMGKPCSSCPPSYQGSCNSNMC 241
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Human
Match: PI15 (peptidase inhibitor 15 [Source:HGNC Symbol;Acc:HGNC:8946])

HSP 1 Score: 79.337 bits (194), Expect = 8.716e-16
Identity = 51/185 (27.57%), Postives = 79/185 (42.70%), Query Frame = 2 
Query:  125 EFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH-----RKLDGYGENGRLASDSNVTNTVMSWFQE--RIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFY---NSGQKDQPYEIGKPCSECSSSY-QFCRDDLCVP 646
              LD HN VR  V+PPA++M+ + ++EN+ KS   +   C ++H      +  G   + R     ++   V  W+ E     F    +   RC              C HY  MV +    +GC    C+N+   G  +  +    C Y    +   + PY++G PCS C  SY   C D+LC P
Sbjct:   69 AILDYHNQVRGKVFPPAANMEYMVWDENLAKSAEAWAATCIWDHGPSYLLRFLGQNLSVRTGRYRSILQLVKPWYDEVKDYAFPYPQDCNPRCPMR------CFGPMCTHYTQMVWATSNRIGCAIHTCQNMNVWGSVWRRAVYLVCNYAPKGNWIGEAPYKVGVPCSSCPPSYGGSCTDNLCFP 247
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Celegans
Match: lon-1 (pep chromosome:WBcel235:III:5474478:5477999:1 gene:WBGene00003055.1 transcript:F48E8.1c.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:lon-1)

HSP 1 Score: 62.3882 bits (150), Expect = 2.040e-10
Identity = 51/183 (27.87%), Postives = 80/183 (43.72%), Query Frame = 2 
Query:  119 RKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH-RKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQG-NGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYD 661
            +K     HN  RR V  PASDM  L +++ +  S   + + C F H R     GEN   A  SN ++ +  WF E            RC  +++     ++  CGHY  +V +    +GCG+  C+++QG  G  + + ++  C YN   K         CS C ++   C   LC     Y+
Sbjct:   81 KKWITHEHNRYRRMV--PASDMNMLYWSDELAASAQRHADTCDFRHSRGRINVGENIWAAPYSNYSDAISIWFNE--------VHNPRCGCNHA-----YKHCCGHYVQVVWAKTNLVGCGFSRCRDVQGVWGRGHRNVFV--CHYNPQGK---------CSNCPANAPACYQGLCYMPKNYE 237
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Celegans
Match: lon-1 (pep chromosome:WBcel235:III:5474478:5477999:1 gene:WBGene00003055.1 transcript:F48E8.1b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:lon-1)

HSP 1 Score: 62.3882 bits (150), Expect = 2.752e-10
Identity = 54/186 (29.03%), Postives = 84/186 (45.16%), Query Frame = 2 
Query:  119 RKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH-RKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQG-NGISYTDSYISFCFYNSGQKDQPY------EIGKPCSECSSSYQFCRDDLC-VPK 649
            +K     HN  RR V  PASDM  L +++ +  S   + + C F H R     GEN   A  SN ++ +  WF E            RC  +++     ++  CGHY  +V +    +GCG+  C+++QG  G  + + ++  C YN      P       + GK CS C ++   C   LC +PK
Sbjct:   81 KKWITHEHNRYRRMV--PASDMNMLYWSDELAASAQRHADTCDFRHSRGRINVGENIWAAPYSNYSDAISIWFNE--------VHNPRCGCNHA-----YKHCCGHYVQVVWAKTNLVGCGFSRCRDVQGVWGRGHRNVFV--CHYNPQGNTMPAFTWASGDNGK-CSNCPANAPACYQGLCYMPK 248
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Celegans
Match: lon-1 (pep chromosome:WBcel235:III:5474478:5477999:1 gene:WBGene00003055.1 transcript:F48E8.1a.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:lon-1)

HSP 1 Score: 58.151 bits (139), Expect = 7.366e-9
Identity = 54/200 (27.00%), Postives = 85/200 (42.50%), Query Frame = 2 
Query:  119 RKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH-RKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQG-NGISYTDSYISFCFYNS-------GQKDQPYEI----------GKPCSECSSSYQFCRDDLCVPKSRYD 661
            +K     HN  RR V  PASDM  L +++ +  S   + + C F H R     GEN   A  SN ++ +  WF E            RC  +++     ++  CGHY  +V +    +GCG+  C+++QG  G  + + ++  C YN          + Q Y +          GK CS C ++   C   LC     Y+
Sbjct:   81 KKWITHEHNRYRRMV--PASDMNMLYWSDELAASAQRHADTCDFRHSRGRINVGENIWAAPYSNYSDAISIWFNE--------VHNPRCGCNHA-----YKHCCGHYVQVVWAKTNLVGCGFSRCRDVQGVWGRGHRNVFV--CHYNPQGNTVFVTARGQLYAMPAFTWASGDNGK-CSNCPANAPACYQGLCYMPKNYE 262
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Celegans
Match: lon-1 (pep chromosome:WBcel235:III:5474478:5478005:1 gene:WBGene00003055.1 transcript:F48E8.1a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:lon-1)

HSP 1 Score: 58.151 bits (139), Expect = 7.366e-9
Identity = 54/200 (27.00%), Postives = 85/200 (42.50%), Query Frame = 2 
Query:  119 RKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH-RKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQG-NGISYTDSYISFCFYNS-------GQKDQPYEI----------GKPCSECSSSYQFCRDDLCVPKSRYD 661
            +K     HN  RR V  PASDM  L +++ +  S   + + C F H R     GEN   A  SN ++ +  WF E            RC  +++     ++  CGHY  +V +    +GCG+  C+++QG  G  + + ++  C YN          + Q Y +          GK CS C ++   C   LC     Y+
Sbjct:   81 KKWITHEHNRYRRMV--PASDMNMLYWSDELAASAQRHADTCDFRHSRGRINVGENIWAAPYSNYSDAISIWFNE--------VHNPRCGCNHA-----YKHCCGHYVQVVWAKTNLVGCGFSRCRDVQGVWGRGHRNVFV--CHYNPQGNTVFVTARGQLYAMPAFTWASGDNGK-CSNCPANAPACYQGLCYMPKNYE 262
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Celegans
Match: scl-1 (SCP-Like extracellular protein [Source:UniProtKB/TrEMBL;Acc:Q20608])

HSP 1 Score: 53.1434 bits (126), Expect = 9.941e-8
Identity = 37/140 (26.43%), Postives = 56/140 (40.00%), Query Frame = 2 
Query:  104 LTPRERKEFLDLHNHVRRSVYP-----------PASDMQELEYNENIEKSVNEYLEQCKFEHRKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFC 490
             T   +   +D HN +R ++             PA+DM+++  +  +  S   Y   C   H K  GYGEN   +  S    ++ S + E        E +    KSN+    L     GH   M  +N  S+GCG K C
Sbjct:   16 FTSTGQSSIVDAHNKLRSAIAKSTYVAKGTKKEPATDMRKMVVDSTVAASAQNYANTCPTGHSKGTGYGENLYWSWTSADVGSLDS-YGEIAAAAWEKEFQDFGWKSNAMDTTLFNSGIGHATQMAWANTSSIGCGVKNC 154
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Fly
Match: CG8483 (gene:FBgn0038126 transcript:FBtr0082782)

HSP 1 Score: 52.373 bits (124), Expect = 9.369e-7
Identity = 53/201 (26.37%), Postives = 85/201 (42.29%), Query Frame = 2 
Query:  101 GLTPRERKEFLDLHNHVRRSVY-------PPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGY--GENGRL--------ASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKD---QPYEIGKP-CS----ECSSSYQ 619
            G+T  ER   L  HN +R+ V        P A +M+E+ +++ +     ++ + C+F H   R ++ +  G+N  +        A D +  + + SWF E   ++    G+    K+            GHY  +V      +GCGY   K+       Y   Y+  C Y  G       PYE+GKP CS    + SS YQ
Sbjct:   31 GITAEERSIILQEHNRLRQIVATGRYPGQPGAENMREIVWDDELAARAQKWADNCQFRHDPHRTINRFTMGQNLAIIWSTAPLDADDGDFPSRIQSWFNEVQKYSF---GDAWSPKT------------GHYSQLVWGETSLVGCGYAEYKDTS----KYNKLYV--CNYGPGGNVVGYNPYEVGKPSCSTYGMKPSSRYQ 210
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Zebrafish
Match: r3hdml (R3H domain containing-like [Source:ZFIN;Acc:ZDB-GENE-090313-275])

HSP 1 Score: 92.0485 bits (227), Expect = 2.169e-20
Identity = 63/199 (31.66%), Postives = 97/199 (48.74%), Query Frame = 2 
Query:  104 LTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRK---LDGYGENGRLASD--SNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFY---NSGQKDQPYEIGKPCSECSSSY-QFCRDDLC--VPKSRYDKN 667
            ++ ++    LD HN VR  V+PPA++M+ + ++E + KS   +  QC +EH     L   G+N  + S    ++ + V SW+ ER  F+  +    RCS S           C HY  MV +    +GC  K C +I   G  +  + +  C Y    +   + PY+IG+PCS C SSY   C  + C   PKSR + N
Sbjct:   61 ISGKDMTALLDYHNRVRSQVFPPAANMEYMVWDERLAKSAEFWASQCIWEHGPHHFLQHIGQNLSIISGRYKSIIDLVKSWYDERHSFSYPS----RCSGS----------VCTHYTQMVWAASNKIGCAIKKCSDIFVFGSMWKQATLLVCNYAIKGNWVGEAPYKIGRPCSACPSSYGGSCNKNQCDSRPKSRRNTN 245
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Zebrafish
Match: r3hdml (R3H domain containing-like [Source:ZFIN;Acc:ZDB-GENE-090313-275])

HSP 1 Score: 92.0485 bits (227), Expect = 2.169e-20
Identity = 63/199 (31.66%), Postives = 97/199 (48.74%), Query Frame = 2 
Query:  104 LTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRK---LDGYGENGRLASD--SNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFY---NSGQKDQPYEIGKPCSECSSSY-QFCRDDLC--VPKSRYDKN 667
            ++ ++    LD HN VR  V+PPA++M+ + ++E + KS   +  QC +EH     L   G+N  + S    ++ + V SW+ ER  F+  +    RCS S           C HY  MV +    +GC  K C +I   G  +  + +  C Y    +   + PY+IG+PCS C SSY   C  + C   PKSR + N
Sbjct:   61 ISGKDMTALLDYHNRVRSQVFPPAANMEYMVWDERLAKSAEFWASQCIWEHGPHHFLQHIGQNLSIISGRYKSIIDLVKSWYDERHSFSYPS----RCSGS----------VCTHYTQMVWAASNKIGCAIKKCSDIFVFGSMWKQATLLVCNYAIKGNWVGEAPYKIGRPCSACPSSYGGSCNKNQCDSRPKSRRNTN 245
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Zebrafish
Match: crispld2 (cysteine-rich secretory protein LCCL domain containing 2 [Source:ZFIN;Acc:ZDB-GENE-130131-1])

HSP 1 Score: 83.5741 bits (205), Expect = 2.194e-16
Identity = 57/193 (29.53%), Postives = 89/193 (46.11%), Query Frame = 2 
Query:  116 ERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLD---GYGEN-----GRLASDSNVTNTVMSWFQERIYFNISAEGE------GRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQK---DQPYEIGKPCSECSSSY-QFCRDDLC 640
            +R+E L LHN +R  VYP AS+M+ + +++ +E+S   + EQC++EH   D     G+N     GR  S +     V +W+ E   +      E       RCS             C HY  +V +    +GC    C  +   G  + ++    C Y+       + PY+ G+PCS+C  SY   CRD+LC
Sbjct:   64 DREEILKLHNKLRGEVYPTASNMEYMIWDDELERSATSWAEQCQWEHGPQDLLMSIGQNLAVHWGRYRSPA---YHVQAWYDEVKDYTYPYPHECNPWCPERCSGP----------MCTHYTQLVWATTNRVGCAVHVCPRMNVWGEIWENAVYLVCNYSPKGNWIGEAPYQHGRPCSQCPPSYGGVCRDNLC 243
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Zebrafish
Match: crispld1a (cysteine-rich secretory protein LCCL domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-090612-1])

HSP 1 Score: 82.8037 bits (203), Expect = 2.517e-16
Identity = 62/212 (29.25%), Postives = 94/212 (44.34%), Query Frame = 2 
Query:  101 GLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRK---LDGYGEN-----GRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPF-LHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYN---SGQKDQPYEIGKPCSECSSSY-QFCRDDLCVPKSRYDKNSRTCKLKCRN 697
             +T  + +  LDLHN +R  VYPPAS+M+ + ++  +E+S  E+ E C +EH     L   G+N     GR       T+ V +W+ E   ++     E      N   PF      C HY  +V +    +GC    C N+   G  +  +    C Y+   +     PY+ G  CS C  SY   CR++LC     Y   + TC  K R+
Sbjct:   61 AITASDMQAILDLHNKLRGQVYPPASNMEYMVWDNELERSAEEWAETCLWEHGPAGLLPQIGQNLGVHWGRYRPP---TSHVQAWYDEVKDYSFPYPQE-----CNPHCPFRCSGPVCTHYTQLVWATSSRIGCAINVCYNMNVWGQIWAKAVYLVCNYSPKGNWWGYAPYKHGTSCSACPPSYGGVCRENLC-----YKAQTVTCDTKLRD 259
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Zebrafish
Match: crispld1a (cysteine-rich secretory protein LCCL domain containing 1a [Source:ZFIN;Acc:ZDB-GENE-090612-1])

HSP 1 Score: 79.337 bits (194), Expect = 5.029e-15
Identity = 62/221 (28.05%), Postives = 97/221 (43.89%), Query Frame = 2 
Query:  101 GLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRK---LDGYGEN-----GRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFL-HEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYN---SGQKDQPYEIGKPCSECSSSY-QFCRDDLCVPKSRYDKNSRTCKLKCRNCAEYFDPKN 724
             +T  + +  LDLHN +R  VYPPAS+M+ + ++  +E+S  E+ E C +EH     L   G+N     GR       T+ V +W+ E   ++     E      N   PF      C HY  +V +    +GC    C N+   G  +  +    C Y+   +     PY+ G  CS C  SY   CR++LC      ++ S + +   RN  E   P +
Sbjct:   61 AITASDMQAILDLHNKLRGQVYPPASNMEYMVWDNELERSAEEWAETCLWEHGPAGLLPQIGQNLGVHWGRYRPP---TSHVQAWYDEVKDYSFPYPQE-----CNPHCPFRCSGPVCTHYTQLVWATSSRIGCAINVCYNMNVWGQIWAKAVYLVCNYSPKGNWWGYAPYKHGTSCSACPPSYGGVCRENLCYKGDGKNRQSPSEETHERNSIEPEAPHS 273
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Xenopus
Match: R3HDML (R3H domain containing-like [Source:NCBI gene;Acc:100492715])

HSP 1 Score: 93.5893 bits (231), Expect = 7.757e-21
Identity = 55/196 (28.06%), Postives = 97/196 (49.49%), Query Frame = 2 
Query:  104 LTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGENGRLASD--SNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYN---SGQKDQPYEIGKPCSECSSSY-QFCRDDLCVPKSRYDK 664
            ++PR+    LD HN VR  V+PPA++M+ + ++E + KS   +  QCK++H   + +   G+N  + S    ++ + V  W+ ER +++     E   S  N          C HY  MV ++   +GC    C NI   G ++  +    C Y+   +   + PY++G+PCS C  SY   C +++C    + +K
Sbjct:   53 ISPRDMSALLDYHNQVRSKVFPPAANMEYMVWDERLAKSAESWANQCKWDHGPNQLMRYIGQNLSVHSGRYRSIVDLVKGWYDERQHYSFPHPRECNPSCPNK----CTGAVCTHYTQMVWASSNRIGCAVNICTNINVWGSTWRQASYLVCNYSIKGNWIGEAPYKLGRPCSACPPSYGGVCSNNMCFSGVKSNK 244
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Xenopus
Match: R3HDML (R3H domain containing-like [Source:NCBI gene;Acc:100492715])

HSP 1 Score: 93.5893 bits (231), Expect = 1.037e-20
Identity = 55/196 (28.06%), Postives = 97/196 (49.49%), Query Frame = 2 
Query:  104 LTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGENGRLASD--SNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYN---SGQKDQPYEIGKPCSECSSSY-QFCRDDLCVPKSRYDK 664
            ++PR+    LD HN VR  V+PPA++M+ + ++E + KS   +  QCK++H   + +   G+N  + S    ++ + V  W+ ER +++     E   S  N          C HY  MV ++   +GC    C NI   G ++  +    C Y+   +   + PY++G+PCS C  SY   C +++C    + +K
Sbjct:   58 ISPRDMSALLDYHNQVRSKVFPPAANMEYMVWDERLAKSAESWANQCKWDHGPNQLMRYIGQNLSVHSGRYRSIVDLVKGWYDERQHYSFPHPRECNPSCPNK----CTGAVCTHYTQMVWASSNRIGCAVNICTNINVWGSTWRQASYLVCNYSIKGNWIGEAPYKLGRPCSACPPSYGGVCSNNMCFSGVKSNK 249
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Xenopus
Match: ENSXETT00000036788.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:KV464547.1:593:5826:1 gene:ENSXETG00000015856.1 transcript:ENSXETT00000036788.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 87.4261 bits (215), Expect = 7.635e-18
Identity = 60/187 (32.09%), Postives = 90/187 (48.13%), Query Frame = 2 
Query:   98 LGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDGY-GENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFY---NSGQKDQPYEIGKPCSECSSSYQFCRDDLC 640
            L L   E++  LD HN  R    P ASDM +L ++  +E     Y E+C +EH K  G+ GEN  + S S  +V   +  W +ER Y+N +    G C +            CGHY  MV +  + +GCG  FC  ++  G+   + Y+  C Y    + + + PY+ G  C+EC S +  C   LC
Sbjct:   28 LALNYEEKRIILDKHNFYRSQTEPSASDMIKLTWDSALEAMAKSYAEKCIWEHNKDRGFIGENLFVMSGSSLDVALGLDDWHKERGYYNFTT---GMCQEGQ---------MCGHYTQMVWAGTERVGCGQNFCPKLE--GVDDENMYLLVCNYEPPGNFEGESPYKEGSRCTECPSDH-VCIGSLC 199
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Xenopus
Match: CRISPLD1 (cysteine-rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:100490431])

HSP 1 Score: 79.337 bits (194), Expect = 4.402e-15
Identity = 58/192 (30.21%), Postives = 84/192 (43.75%), Query Frame = 2 
Query:  101 GLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLD-----GYGENGRLASDSNVTNTVMSWFQE-RIY-FNISAEGEGRCSKSNSKIPFL-HEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYN---SGQKDQPYEIGKPCSECSSSY-QFCRDDLC 640
             +T  + K  LDLHN +R  VYPPAS+M+ + ++  +E+S   + E C +EH   D     G             T  V +W+ E R Y F    E +  C       PF      C HY  +V +    +GC    C N+   G  +  +    C Y+   +     PY+ G PCS C  SY   C+D+LC
Sbjct:   56 AITESDMKLILDLHNKLRGEVYPPASNMEFMIWDVELERSAEAWAETCLWEHGPADLLPVIGQNLGAHWGRYRPPTYHVQAWYDEVRDYTFPYPQECDPYC-------PFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMNVWGQIWPKAIYLVCNYSPKGNWWGHAPYKHGHPCSACPPSYGGGCKDNLC 240
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Xenopus
Match: R3HDML (R3H domain containing-like [Source:NCBI gene;Acc:100492715])

HSP 1 Score: 75.485 bits (184), Expect = 1.072e-14
Identity = 51/197 (25.89%), Postives = 94/197 (47.72%), Query Frame = 2 
Query:  104 LTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGENGRLASD--SNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQ----KDQPYEIGKPCSECSSSY-QFCRDDLCVPKSRYDK 664
            ++PR+    LD HN VR  V+PPA++M+ + ++E + KS   +  QCK++H   + +   G+N  + S    ++ + V  W+ ER +++     E   S  N          C HY  ++  +L S+              ++Y+ + I+    NS       + PY++G+PCS C  SY   C +++C    + +K
Sbjct:   58 ISPRDMSALLDYHNQVRSKVFPPAANMEYMVWDERLAKSAESWANQCKWDHGPNQLMRYIGQNLSVHSGRYRSIVDLVKGWYDERQHYSFPHPRECNPSCPNKCT----GAVCTHYTQVLDESLLSV--------------LAYSGNSINVRNVNSAHGNWIGEAPYKLGRPCSACPPSYGGVCSNNMCFSGVKSNK 236
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 94.7449 bits (234), Expect = 4.233e-21
Identity = 61/200 (30.50%), Postives = 94/200 (47.00%), Query Frame = 2 
Query:   83 ALYQILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDG-YGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSG---QKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDK 664
            A      LT  E++  +DLHN  R  V PPASDM ++ +++ +      Y ++C + H K  G  GEN    +D   +V   V +W +E  Y+N S      C  +           CGHY  +V S  + +GCG  FC+ +Q  G+   + ++  C Y      +  +PY+ G PCS+C   Y  C + LC P    +K
Sbjct:   23 ATGPTTALTEDEKQTMVDLHNQYRAQVSPPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRRGENLFAITDEGMDVPLAVGNWHEEHEYYNFST---ATCDPNQ---------MCGHYTQVVWSKTERIGCGSHFCETLQ--GVEEANIHLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYS-CENSLCEPMRNPEK 207
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 94.7449 bits (234), Expect = 4.233e-21
Identity = 61/200 (30.50%), Postives = 94/200 (47.00%), Query Frame = 2 
Query:   83 ALYQILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDG-YGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSG---QKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDK 664
            A      LT  E++  +DLHN  R  V PPASDM ++ +++ +      Y ++C + H K  G  GEN    +D   +V   V +W +E  Y+N S      C  +           CGHY  +V S  + +GCG  FC+ +Q  G+   + ++  C Y      +  +PY+ G PCS+C   Y  C + LC P    +K
Sbjct:   23 ATGPTTALTEDEKQTMVDLHNQYRAQVSPPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRRGENLFAITDEGMDVPLAVGNWHEEHEYYNFST---ATCDPNQ---------MCGHYTQVVWSKTERIGCGSHFCETLQ--GVEEANIHLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYS-CENSLCEPMRNPEK 207
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 92.4337 bits (228), Expect = 1.199e-20
Identity = 62/212 (29.25%), Postives = 97/212 (45.75%), Query Frame = 2 
Query:  101 GLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDG-YGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSG---QKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDKNSRTCKLKCRNCAEYFDP 718
             LT  E++  +DLHN  R  V PPASDM ++ +++ +      Y ++C + H K  G  GEN    +D   +V   V +W +E  Y+N S      C  +           CGHY  +V S  + +GCG  FC+ +Q  G+   + ++  C Y      +  +PY+ G PCS+C   Y  C + LC       +N        +N A  + P
Sbjct:   29 ALTEDEKQTMVDLHNQYRAQVSPPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRRGENLFAITDEGMDVPLAVGNWHEEHEYYNFST---ATCDPNQ---------MCGHYTQVVWSKTERIGCGSHFCETLQ--GVEEANIHLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYS-CENSLCGA-----ENPEKADWDLKNSAHVWGP 220
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Mouse
Match: R3hdml (R3H domain containing-like [Source:MGI Symbol;Acc:MGI:3650937])

HSP 1 Score: 92.4337 bits (228), Expect = 1.602e-20
Identity = 61/207 (29.47%), Postives = 103/207 (49.76%), Query Frame = 2 
Query:   83 ALYQILGL-TPRERKE----------FLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGENGRLASD--SNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFL-HEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFY---NSGQKDQPYEIGKPCSECSSSYQF-CRDDLC 640
            A++ + GL  PR R++           LD HNH+R SV+PPA++M+ + ++E + +S   +  QC + H   + +   G+N  + S    +V + V SW +E+ +++  A  +  C+      P+L     C HY  MV ++   LGC    C +I   G ++  +    C Y    +   + PY+ GKPCS C  SYQ  C  ++C
Sbjct:   40 AVWPLSGLGVPRHRRKRHISARDMSALLDYHNHIRASVHPPAANMEYMVWDEQLARSAEAWATQCIWTHGPSQLMKYVGQNLSIHSGRFRSVVDLVRSWSEEKRHYSFPAPKD--CTP---HCPWLCSGPVCSHYTQMVWASSSRLGCAINTCSSINVWGNTWQQAVYLVCNYAIKGNWIGEAPYKAGKPCSACPPSYQGNCNSNMC 241
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Mouse
Match: Pi16 (peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:1921366])

HSP 1 Score: 93.9745 bits (232), Expect = 8.877e-20
Identity = 61/200 (30.50%), Postives = 94/200 (47.00%), Query Frame = 2 
Query:   83 ALYQILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDG-YGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSG---QKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDK 664
            A      LT  E++  +DLHN  R  V PPASDM ++ +++ +      Y ++C + H K  G  GEN    +D   +V   V +W +E  Y+N S      C  +           CGHY  +V S  + +GCG  FC+ +Q  G+   + ++  C Y      +  +PY+ G PCS+C   Y  C + LC P    +K
Sbjct:   23 ATGPTTALTEDEKQTMVDLHNQYRAQVSPPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRRGENLFAITDEGMDVPLAVGNWHEEHEYYNFST---ATCDPNQ---------MCGHYTQVVWSKTERIGCGSHFCETLQ--GVEEANIHLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYS-CENSLCEPMRNPEK 207
[Close]
BLAST of Peptidase inhibitor 16 vs. UniProt/SwissProt
Match: sp|A2A5I3|CRSPL_MOUSE (Peptidase inhibitor R3HDML OS=Mus musculus OX=10090 GN=R3hdml PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.121e-19
Identity = 61/207 (29.47%), Postives = 103/207 (49.76%), Query Frame = 2 
Query:   83 ALYQILGL-TPRERKE----------FLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGENGRLASD--SNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFL-HEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFY---NSGQKDQPYEIGKPCSECSSSYQF-CRDDLC 640
            A++ + GL  PR R++           LD HNH+R SV+PPA++M+ + ++E + +S   +  QC + H   + +   G+N  + S    +V + V SW +E+ +++  A  +  C+      P+L     C HY  MV ++   LGC    C +I   G ++  +    C Y    +   + PY+ GKPCS C  SYQ  C  ++C
Sbjct:   40 AVWPLSGLGVPRHRRKRHISARDMSALLDYHNHIRASVHPPAANMEYMVWDEQLARSAEAWATQCIWTHGPSQLMKYVGQNLSIHSGRFRSVVDLVRSWSEEKRHYSFPAPKD--CTP---HCPWLCSGPVCSHYTQMVWASSSRLGCAINTCSSINVWGNTWQQAVYLVCNYAIKGNWIGEAPYKAGKPCSACPPSYQGNCNSNMC 241
[Close]
BLAST of Peptidase inhibitor 16 vs. UniProt/SwissProt
Match: sp|Q6UXB8|PI16_HUMAN (Peptidase inhibitor 16 OS=Homo sapiens OX=9606 GN=PI16 PE=1 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.574e-19
Identity = 61/190 (32.11%), Postives = 92/190 (48.42%), Query Frame = 2 
Query:   95 ILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDG-YGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSG---QKDQPYEIGKPCSECSSSYQFCRDDLCVP 646
            +  LT  E++  ++LHN  R  V P ASDM  + ++E +      Y  QC + H K  G  GEN    +D   +V   +  W  ER ++N+SA     CS             CGHY  +V +  + +GCG  FC+ +Q  G+  T+  +  C Y      +  +PY+ G PCS+C S Y  C++ LC P
Sbjct:   25 VGALTDEEKRLMVELHNLYRAQVSPTASDMLHMRWDEELAAFAKAYARQCVWGHNKERGRRGENLFAITDEGMDVPLAMEEWHHEREHYNLSA---ATCSPGQ---------MCGHYTQVVWAKTERIGCGSHFCEKLQ--GVEETNIELLVCNYEPPGNVKGKRPYQEGTPCSQCPSGYH-CKNSLCEP 199
[Close]
BLAST of Peptidase inhibitor 16 vs. UniProt/SwissProt
Match: sp|Q9ET66|PI16_MOUSE (Peptidase inhibitor 16 OS=Mus musculus OX=10090 GN=Pi16 PE=1 SV=2)

HSP 1 Score: 93.9745 bits (232), Expect = 6.210e-19
Identity = 61/200 (30.50%), Postives = 94/200 (47.00%), Query Frame = 2 
Query:   83 ALYQILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDG-YGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSG---QKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDK 664
            A      LT  E++  +DLHN  R  V PPASDM ++ +++ +      Y ++C + H K  G  GEN    +D   +V   V +W +E  Y+N S      C  +           CGHY  +V S  + +GCG  FC+ +Q  G+   + ++  C Y      +  +PY+ G PCS+C   Y  C + LC P    +K
Sbjct:   23 ATGPTTALTEDEKQTMVDLHNQYRAQVSPPASDMLQMRWDDELAAFAKAYAQKCVWGHNKERGRRGENLFAITDEGMDVPLAVGNWHEEHEYYNFST---ATCDPNQ---------MCGHYTQVVWSKTERIGCGSHFCETLQ--GVEEANIHLLVCNYEPPGNVKGRKPYQEGTPCSQCPLGYS-CENSLCEPMRNPEK 207
[Close]
BLAST of Peptidase inhibitor 16 vs. UniProt/SwissProt
Match: sp|Q9H3Y0|CRSPL_HUMAN (Peptidase inhibitor R3HDML OS=Homo sapiens OX=9606 GN=R3HDML PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 4.198e-18
Identity = 53/186 (28.49%), Postives = 93/186 (50.00%), Query Frame = 2 
Query:  113 RERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGENGRLASDS--NVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHE-VQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFY---NSGQKDQPYEIGKPCSECSSSYQ-FCRDDLC 640
            R+    LD HNH+R SVYPPA++M+ + +++ + ++   +  QC + H   + +   G+N  + S    +V + + SW +E+ ++   A  +      N   P+  +   C HY  MV ++   LGC    C +I   G ++  +    C Y    +   + PY++GKPCS C  SYQ  C  ++C
Sbjct:   61 RDMNALLDYHNHIRASVYPPAANMEYMVWDKRLARAAEAWATQCIWAHGPSQLMRYVGQNLSIHSGQYRSVVDLMKSWSEEKWHYLFPAPRD-----CNPHCPWRCDGPTCSHYTQMVWASSNRLGCAIHTCSSISVWGNTWHRAAYLVCNYAIKGNWIGESPYKMGKPCSSCPPSYQGSCNSNMC 241
[Close]
BLAST of Peptidase inhibitor 16 vs. UniProt/SwissProt
Match: sp|Q58D34|PI16_BOVIN (Peptidase inhibitor 16 OS=Bos taurus OX=9913 GN=PI16 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.110e-17
Identity = 57/185 (30.81%), Postives = 90/185 (48.65%), Query Frame = 2 
Query:  116 ERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDG-YGEN--GRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNS-----GQKDQPYEIGKPCSECSSSYQFCRDDLCVP 646
            E+   ++LHN  R  V PPA++M ++ ++E +      Y +QC + H K  G  GEN         +V   +  W  ER ++N+SA     C+             CGHY  +V +  + +GCG  FC+ +Q  G+  T+ ++  C Y       GQ+  PY+ G PCS+C   Y  C++ LC P
Sbjct:   32 EKHVMVELHNLYRTQVSPPATNMLQMRWDEELAAFAKAYAQQCVWGHNKERGRRGENLFAITGEGLDVPLAMEEWHHEREHYNLSAIS---CAAGQ---------MCGHYTQVVWAKTERIGCGSHFCEKLQ--GVEETNIHLLVCNYEPPGNVKGQR--PYQEGTPCSQCPLGYH-CKNSLCEP 199
[Close]
BLAST of Peptidase inhibitor 16 vs. TrEMBL
Match: A0A1I8HLS1 (SCP domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 4.075e-30
Identity = 98/331 (29.61%), Postives = 149/331 (45.02%), Query Frame = 2 
Query:  104 LTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHR---KLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSK--SNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDKNSRTCKLKCRNCAEYFDPKNSCLCDCPDGTEGNSCRGTPAVVDENCPSCLAQGLSC-NSGTEDSRS-GNCNCKCTPEYIGEYCEIRKDQFDSSFYYVVDGTDWRYNMMIPVNAIEFKKSFYKFQNAIARLINEFCVKNL 1075
            +T  E +  ++LHN +RRSV P A++M +L  +  +     +  + C+FEH       G GEN  +   SN +  VM WF ER  + +SA G   C     N + P  +   CGHY  +V+++++ +GCG K C          + S +  C YNSG    PY  G  CS CSS+   C D LCVP      +  TC L C++C +              G    SC  + + V   C  C +    C N+GT+   S  NC+C C  ++ G+ C+  + QF S+      G + R     PV    +   +   +  +   I +FC  N 
Sbjct:    1 MTDAEHQGIVELHNRLRRSVLPEAANMNQLIASATLRNLAQQAADTCRFEHNYGGDYSGLGENLYMG-GSNASLAVMLWFIERENYRLSASGT-LCGLEYGNGRYP-QYASSCGHYYQVVQASVREVGCGKKAC----------SGSSLIVCEYNSGSGSPPYTAGPSCSGCSSARPRCLDGLCVP---VGTSGSTCSLACKSCTKLV------------GNATGSCDCSGSYVP--CTKCDSDATQCLNNGTDVGASLTNCSCVCPTDFYGDRCQYNRAQFASALQLRFGG-ELRSRSYFPVPKSFWPSLWRALEPPLLTAIADFCRWNF 300
[Close]
BLAST of Peptidase inhibitor 16 vs. TrEMBL
Match: A0A1I8GC84 (SCP domain-containing protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 7.063e-30
Identity = 93/353 (26.35%), Postives = 152/353 (43.06%), Query Frame = 2 
Query:  101 GLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDK------------NSRTCKLKCRNC--AEYFDPKNSCLCDCPDGT-EGNSCRGTPAVVDENCPSCLAQGLSCNSGTEDSRSGNCNCKCTPEYIGEYCEIRKDQFDSSFYYVVDG---TDWRYNMMIPVNAIEFKKSFY-KFQNAIARLINEFCVKNLNSYPEC 1093
             L+  ++ EF+++HN +R +VYP A+ M  L +N  +E       E+CKFEH      +G GEN  +   +NV+   ++W  E+  +N +A          S  P      CGHYK ++   +  +GCG+  C          + S + FC Y+SG K      G  CS+C S    C + +CV  S   +            +S  CKL C NC  ++       C C C  G   G  C    A     C  C      C +G  D +  +C C+C   ++G  C++ +  + +     +       +   + +PV    FK S + + + A+A+ IN FC  +  ++  C
Sbjct:   33 ALSASQKTEFVNIHNRLREAVYPRATKMNFLTWNTRLESLAQAASEKCKFEHYYGGDYEGLGENIYMGYRTNVSEITLAWHIEKAIYNYTAA---------SCNPAGKLTTCGHYKQLISDGVLEVGCGHYVC----------SGSNLVFCEYSSGSKVYT-SGGSACSKCPSYRGLCLNGMCVNSSIVSQSDVQAYNPGAKVSSTECKLNCSNCGVSQSIPGSLDCYCKCASGIGTGAYCENRVADSKSTCSVCSNLTTPCLNGGTDLKGASCACQCPATFVGPRCQVYRQFYLNGMRLSIIAEQFCAFDRCLQVPVTRTMFKTSIWSRLRPAVAQAINAFCTASEANFALC 365
[Close]
BLAST of Peptidase inhibitor 16 vs. TrEMBL
Match: A0A267DUN5 (SCP domain-containing protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig033476g2 PE=4 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 1.016e-29
Identity = 93/353 (26.35%), Postives = 152/353 (43.06%), Query Frame = 2 
Query:  101 GLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDK------------NSRTCKLKCRNC--AEYFDPKNSCLCDCPDGT-EGNSCRGTPAVVDENCPSCLAQGLSCNSGTEDSRSGNCNCKCTPEYIGEYCEIRKDQFDSSFYYVVDGTD---WRYNMMIPVNAIEFKKSFY-KFQNAIARLINEFCVKNLNSYPEC 1093
             L+  ++ EF+++HN +R +VYP A+ M  L +N  +E       E+CKFEH      +G GEN  +   +NV+   ++W  E+  +N +A          S  P      CGHYK ++   +  +GCG+  C          + S + FC Y+SG K      G  CS+C S    C + +CV  S   +            +S  CKL C NC  ++       C C C  G   G  C    A     C  C      C +G  D +  +C C+C   ++G  C++ +  + +     +       +   + +PV    FK S + + + A+A+ IN FC  +  ++  C
Sbjct:   49 ALSASQKTEFVNIHNRLREAVYPRATKMNFLTWNTRLESLAQAASEKCKFEHYYGGDYEGLGENIYMGYRTNVSEITLAWHIEKAIYNYTAA---------SCNPAGKLTTCGHYKQLISDGVLEVGCGHYVC----------SGSNLVFCEYSSGSKVYT-SGGSACSKCPSYRGLCLNGMCVNSSIVSQSDVQAYNPGAKVSSTECKLNCSNCGVSQSIPGSLDCYCKCASGIGTGAYCENRVADSKSTCSVCSNLTTPCLNGGTDLKGASCACQCPATFVGPRCQVYRQFYLNGMRLSIIAEQFCAFDRCLQVPVTRTMFKTSIWSRLRPAVAQAINAFCTASEANFALC 381
[Close]
BLAST of Peptidase inhibitor 16 vs. TrEMBL
Match: A0A267H701 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000644g4 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 1.491e-28
Identity = 111/444 (25.00%), Postives = 190/444 (42.79%), Query Frame = 2 
Query:   62 ILAFVECALYQILG---LTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDGY---GENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQ-FCRDDLC----VPKSRYDKNSRTCKLKCRNCAEYFDPKNSCLCDCPDGTEGNSCRGTPAVVDENCPS-CLAQGLSCNSGTEDSRSG-NCNCKCTPEYIGEYCEIRKDQFDSSFYYVVDGTDWRYNMMIPVNAIEFKKSFYK--FQNAIARLINEFCVKNL------NSYPECKKIEENQIIYDNVLLIPGFPHLLENGSLAIRIFIIFNDVTDNV---KQERFVSTTLLREILTNSDDIKNLIENVRKVVL 1321
            + AF+  AL   +G   LT +ER + L+  NH+RR+  PPA+DM  L ++  +E         C FEH     Y   GE   L S  N +  ++  F ER  +N +    G C   + K PF     CGHY  +V + +  +GCG ++C          + +Y+  C ++      PY+ G+  ++C + Y  + +D LC    V ++        CKL C N          C C CP G  G  C           P+ C AQ ++C +G   + +G  C C C   + GE C+I K Q +S+   +  G + RY+   P+    +  +  K   +  +   IN +C  N       ++        ++    ++  +   +P    +GS+ + ++       DNV   K   +VS  +++   T +  +KN+   +  + L
Sbjct:    9 LCAFLAFALAIQIGRCKLTEQERNDTLNFINHLRRNAVPPAADMNGLIWDTRLENLAQRAANSCVFEHNYGGDYSSLGEILYLGSRQNFSEILVFMFLEREQYNYT---NGACVTGSGKYPFFS--TCGHYLQIVTAGVNKVGCGIQYC----------SGNYLGVCEFDREWYVPPYQTGEAGTKCGNGYATYFKDGLCYSILVGETPPIPGPFECKLSCLNYGTLMTSP-FCSCSCPAGYYGVRCETKNIYFASTIPNDCKAQAVTCKNGGSPTMTGTTCKCVCPANFEGESCQINKVQ-ESNSLAIYFGGEMRYSSYYPIKMSIWNDTLLKESTRTHLTDAINSYCNANFSTCCPSSTASSLPATIQHFATCEDFHVADSYPIQAIDGSMRVDVYYTMQPSMDNVCNGKTNSYVSKAIIKSAWTTA--MKNINAAISAIGL 433
[Close]
BLAST of Peptidase inhibitor 16 vs. TrEMBL
Match: A0A267FXP2 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig000644g6 PE=4 SV=1)

HSP 1 Score: 130.954 bits (328), Expect = 1.501e-28
Identity = 111/444 (25.00%), Postives = 190/444 (42.79%), Query Frame = 2 
Query:   62 ILAFVECALYQILG---LTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDGY---GENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQ-FCRDDLC----VPKSRYDKNSRTCKLKCRNCAEYFDPKNSCLCDCPDGTEGNSCRGTPAVVDENCPS-CLAQGLSCNSGTEDSRSG-NCNCKCTPEYIGEYCEIRKDQFDSSFYYVVDGTDWRYNMMIPVNAIEFKKSFYK--FQNAIARLINEFCVKNL------NSYPECKKIEENQIIYDNVLLIPGFPHLLENGSLAIRIFIIFNDVTDNV---KQERFVSTTLLREILTNSDDIKNLIENVRKVVL 1321
            + AF+  AL   +G   LT +ER + L+  NH+RR+  PPA+DM  L ++  +E         C FEH     Y   GE   L S  N +  ++  F ER  +N +    G C   + K PF     CGHY  +V + +  +GCG ++C          + +Y+  C ++      PY+ G+  ++C + Y  + +D LC    V ++        CKL C N          C C CP G  G  C           P+ C AQ ++C +G   + +G  C C C   + GE C+I K Q +S+   +  G + RY+   P+    +  +  K   +  +   IN +C  N       ++        ++    ++  +   +P    +GS+ + ++       DNV   K   +VS  +++   T +  +KN+   +  + L
Sbjct:   80 LCAFLAFALAIQIGRCKLTEQERNDTLNFINHLRRNAVPPAADMNGLIWDTRLENLAQRAANSCVFEHNYGGDYSSLGEILYLGSRQNFSEILVFMFLEREQYNYT---NGACVTGSGKYPFFS--TCGHYLQIVTAGVNKVGCGIQYC----------SGNYLGVCEFDREWYVPPYQTGEAGTKCGNGYATYFKDGLCYSILVGETPPIPGPFECKLSCLNYGTLMTSP-FCSCSCPAGYYGVRCETKNIYFASTIPNDCKAQAVTCKNGGSPTMTGTTCKCVCPANFEGESCQINKVQ-ESNSLAIYFGGEMRYSSYYPIKMSIWNDTLLKESTRTHLTDAINSYCNANFSTCCPSSTASSLPATIQHFATCEDFHVADSYPIQAIDGSMRVDVYYTMQPSVDNVCNGKTNSYVSKAIIKSAWTTA--MKNINAAISAIGL 504
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Cavefish
Match: ENSAMXT00000036030.1 (peptidase inhibitor 16 [Source:NCBI gene;Acc:103029846])

HSP 1 Score: 84.7297 bits (208), Expect = 4.805e-18
Identity = 60/219 (27.40%), Postives = 100/219 (45.66%), Query Frame = 2 
Query:   38 QLAMNFVFILAFVECALYQILGLTPRE-RKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHR--KLDGYGENGRLASDS-NVTNTVMSWFQERIYF----NISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNS-----GQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSR 655
            ++A  +  I A +   L    G  P+E ++  ++LHNH R +V PPA+DM  ++++  +     EY  +C +EH     +  GEN  + +   ++   +  WF E  ++    N  AE E                 CGHY  MV +  K +GCG   C+ ++  G  + ++ +  C Y       GQK  PY++G+ CS+C    + C D  C    R
Sbjct:    2 KMAPRWTLICAGLWMLLAPGRGQFPKEDQQRIVNLHNHHRSTVEPPAADMIHMKWSGELALIAKEYATKCIWEHNPDSQNIMGENLFITTGPLDIEKALSDWFMEHKHYSYDNNTCAENE----------------MCGHYTQMVWAKTKEVGCGSHLCETVEALG--FKNATLLVCNYLPLGNYMGQK--PYQVGEQCSKCPDDLKNCVDQACDSTDR 200
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Cavefish
Match: ENSAMXT00000029824.1 (peptidase inhibitor 16 [Source:NCBI gene;Acc:103029846])

HSP 1 Score: 84.7297 bits (208), Expect = 9.262e-18
Identity = 59/214 (27.57%), Postives = 99/214 (46.26%), Query Frame = 2 
Query:   38 QLAMNFVFILAFVECALYQILGLTPRE-RKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHR--KLDGYGENGRLASDS-NVTNTVMSWFQERIYF----NISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNS-----GQKDQPYEIGKPCSECSSSYQFCRDDLC 640
            ++A  +  I A +   L    G  P+E ++  ++LHNH R +V PPA+DM  ++++  +     EY  +C +EH     +  GEN  + +   ++   +  WF E  ++    N  AE E                 CGHY  MV +  K +GCG   C+ ++  G  + ++ +  C Y       GQK  PY++G+ CS+C    + C D  C
Sbjct:    2 KMAPRWTLICAGLWMLLAPGRGQFPKEDQQRIVNLHNHHRSTVEPPAADMIHMKWSGELALIAKEYATKCIWEHNPDSQNIMGENLFITTGPLDIEKALSDWFMEHKHYSYDNNTCAENE----------------MCGHYTQMVWAKTKEVGCGSHLCETVEALG--FKNATLLVCNYLPLGNYMGQK--PYQVGEQCSKCPDDLKNCVDQAC 195
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Cavefish
Match: ENSAMXT00000031557.1 (peptidase inhibitor 16 [Source:NCBI gene;Acc:103029846])

HSP 1 Score: 83.9593 bits (206), Expect = 9.724e-18
Identity = 59/214 (27.57%), Postives = 99/214 (46.26%), Query Frame = 2 
Query:   38 QLAMNFVFILAFVECALYQILGLTPRE-RKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHR--KLDGYGENGRLASDS-NVTNTVMSWFQERIYF----NISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNS-----GQKDQPYEIGKPCSECSSSYQFCRDDLC 640
            ++A  +  I A +   L    G  P+E ++  ++LHNH R +V PPA+DM  ++++  +     EY  +C +EH     +  GEN  + +   ++   +  WF E  ++    N  AE E                 CGHY  MV +  K +GCG   C+ ++  G  + ++ +  C Y       GQK  PY++G+ CS+C    + C D  C
Sbjct:    2 KMAPRWTLICAGLWMLLAPGRGQFPKEDQQRIVNLHNHHRSTVEPPAADMIHMKWSGELALIAKEYATKCIWEHNPDSQNIMGENLFITTGPLDIEKALSDWFMEHKHYSYDNNTCAENE----------------MCGHYTQMVWAKTKEVGCGSHLCETVEALG--FKNATLLVCNYLPLGNYMGQK--PYQVGEQCSKCPDDLKNCVDQAC 195
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Cavefish
Match: r3hdml (R3H domain containing like [Source:NCBI gene;Acc:103046222])

HSP 1 Score: 82.8037 bits (203), Expect = 2.271e-17
Identity = 51/179 (28.49%), Postives = 86/179 (48.04%), Query Frame = 2 
Query:  104 LTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGENGRLASD--SNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYN---SGQKDQPYEIGKPCSECSSSY 616
            ++ R+    L+ HN VR  V+PPA++M+ + ++E + KS   +  QC ++H     +   G+N  ++S    +V   V SW  E+  F+       RC+ S           C HY  MV ++   +GC    C N+   G ++  + +  C Y+   +   + PY+ GKPCS C S Y
Sbjct:   62 VSSRDMTALLEYHNRVRSQVFPPAANMEYMVWDERLAKSAESWASQCIWDHGPSHVMKYMGQNLSISSGRYRSVIELVRSWHDEKNSFSY----PNRCTGS----------VCSHYTQMVWASTNRIGCAINRCSNMYVFGSTWKQATLLVCNYSIKGNWVGEAPYKTGKPCSACPSVY 226
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Cavefish
Match: crispld1b (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:103033853])

HSP 1 Score: 85.8853 bits (211), Expect = 2.717e-17
Identity = 60/200 (30.00%), Postives = 88/200 (44.00%), Query Frame = 2 
Query:  110 PRERKEF--------LDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGEN--GRLASDSNVTNTVMSWFQERIYFNISAEGE------GRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYN---SGQKDQPYEIGKPCSECSSSY-QFCRDDLC 640
            PR R+          LDLHN +R  VYPPAS+M+ + ++  +E+S  ++   C +EH     L   G+N       D   T  V +W+ E  YF+     E       RCS             C HY  +V +    +GC    C N+   G+ +  +    C Y+   +     PY+ G PCS C +SY   CRD+LC
Sbjct:   51 PRSRRAISASDMQLILDLHNKLRGQVYPPASNMEYMVWDTELERSAEDWAHTCLWEHGPPHLLRQIGQNLGAHWGRDRPPTFHVQAWYDEVQYFSYPYPQECDPYCPYRCSGP----------VCTHYTQLVWATSNKIGCAINVCYNMNVWGMIWAKAVYLVCNYSPPGNWWGHAPYKHGHPCSACPASYGGGCRDNLC 240
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000374.1 (pep scaffold:Pmarinus_7.0:GL476650:75849:87118:-1 gene:ENSPMAG00000000334.1 transcript:ENSPMAT00000000374.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 75.485 bits (184), Expect = 6.411e-15
Identity = 51/187 (27.27%), Postives = 82/187 (43.85%), Query Frame = 2 
Query:  116 ERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHR---KLDGYGENGR--LASDSNVTNTVMSWFQERIYFNISAEGEGRCSKS---NSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQK---DQPYEIGKPCSECSSSY-QFCRDDLC 640
            ER+   + HN +R SV+P A+DM+ + ++  +      + +QCK+ H    +LD  G+N    + S+  +   V +WF E  ++    E E  CS       + P      C HY  +V +  + +GC    C +I      +  +    C Y+       + PY  G PCS C   Y   CR  LC
Sbjct:    5 ERRALTEYHNRLRASVHPQAADMEHMSWDWELAGMAEAWAKQCKWGHGPSDQLDAIGQNLAVYVGSERPLAYHVQAWFDEEEWYQYPEEKE--CSPECPHTCRGPI-----CTHYTQLVWAESRRVGCAVHTCLDISVWDAVWPYALYLVCNYSPKGNWIGEAPYRQGTPCSRCPPRYGGSCRGGLC 184
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002250.1 (pep scaffold:Pmarinus_7.0:GL477299:1626:22672:-1 gene:ENSPMAG00000002049.1 transcript:ENSPMAT00000002250.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 58.151 bits (139), Expect = 3.693e-10
Identity = 45/176 (25.57%), Postives = 75/176 (42.61%), Query Frame = 2 
Query:  134 DLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQC--KFEHRKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPF-LHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKD---QPYEIGKPCSECSSSY-QFCRDDLC 640
            +LHN +R  V+PP+S+M+ + + + + K    Y  +C    +   +  +       S    +  V +W+ E   +    E E      N   PF  +   C HY  +V +    +GC    C N+   G  +  +    C Y+         PY+ G+ CSEC  SY   CR++LC
Sbjct:    1 ELHNKLRGEVHPPSSNMEYMAWVKKLIKEAARYPGRCLRLMQPMAMIHFLGQPHWLSHRPPSFHVQAWYDEAKDYQYPYEQE-----CNPWCPFRCNGAVCTHYTQLVWATTNRVGCAINTCYNMNVWGQIWEKAVYLVCNYSPKGNWIGLSPYKSGRACSECPPSYGGSCRNNLC 171
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Sea Lamprey
Match: CRISP3 (cysteine rich secretory protein 3 [Source:HGNC Symbol;Acc:HGNC:16904])

HSP 1 Score: 53.5286 bits (127), Expect = 3.452e-8
Identity = 47/201 (23.38%), Postives = 80/201 (39.80%), Query Frame = 2 
Query:  128 FLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH-----RKLDGY--GENGRLASDSNVTN-TVMSWFQERI----YFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFY-----NSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDKNSRTC 679
             +DLHN +RR+V P A +M ++ + +    +   +   C F H     RK   +  GEN  ++S     N  +  W+ E I     +++ +   G                 GHY  +V  N   LGC   +C    G        Y+  C Y      + + ++PY++G  C+ C  +   C ++LC    +Y      C
Sbjct:   47 IVDLHNDLRRNVTPSAGNMLQMMWKKEAYLNALSWANNCTFGHSPDARRKTTQWTCGENIFMSSAPFTWNYAIRDWYNEVISPGFQYDVGSTTTG---------------AVGHYTQLVWYNSFQLGCAVNYCPKNPG-----VAKYLYVCHYCPMGNLNTRINRPYDLGTSCASCPKN---CVNNLCDNPCKYKNGYANC 224
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Medaka
Match: ENSORLT00000020674.2 (cysteine-rich venom protein pseudecin-like [Source:NCBI gene;Acc:101157728])

HSP 1 Score: 80.4925 bits (197), Expect = 1.306e-16
Identity = 56/202 (27.72%), Postives = 95/202 (47.03%), Query Frame = 2 
Query:   77 ECALYQILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH-----RKLDGY--GENGRLASDSNVTNTVM-SWFQERIYF---NISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFC-FYNSG--QKDQPYEIGKPCSECSSSYQFCRDDLC 640
             C L  I       + E +D+HN  RR+V PPA+DM  + Y+E +  S   ++++C  +H     R L+GY  GEN   ++   +  +++ +W++E  +F   N S  G+                  GHY  +V ++   +GCG   C N         + Y+  C +Y +G  ++  PY++G PC+ C +    C D LC
Sbjct:   17 ACVLADICPEDTAVQAEIVDVHNAFRRAVEPPAADMLMMSYSEELAVSAQAWVDKCVLDHGPPSSRMLNGYELGENLFYSTSPMLWKSIINAWYKEVSHFAFPNASTNGK----------------PVGHYTQVVWNSSYKVGCGVTLCSN---------NIYLYGCHYYRAGNFKRWPPYKVGTPCASCPND---CVDKLC 190
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Medaka
Match: crispld1a (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:101172850])

HSP 1 Score: 83.5741 bits (205), Expect = 1.949e-16
Identity = 58/195 (29.74%), Postives = 86/195 (44.10%), Query Frame = 2 
Query:  101 GLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRK---LDGYGEN--GRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFL-HEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYN---SGQKDQPYEIGKPCSECSSSY-QFCRDDLCVPKSR 655
             +T  + +  LDLHN +R  VYPPAS+M+ + ++  +E+S  E+ E C +EH     L   G+N           T  V +W+ E   ++     E      N   PF      C HY  +V +    +GC    C N+   G  +  +    C Y+   +     PY+ G PCS C  SY   CRD+LC    R
Sbjct:   55 AITDSDAQLILDLHNKLRGQVYPPASNMEYMMWDTELERSAEEWAETCLWEHGPASLLPQIGQNLGAHWGRYQPPTFHVQAWYDEVKDYSFPYPQE-----CNPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMDVWGQIWAKAVYLVCNYSPKGNWWGHAPYKHGTPCSACPPSYGGGCRDNLCYKDDR 244
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Medaka
Match: crispld1a (cysteine rich secretory protein LCCL domain containing 1 [Source:NCBI gene;Acc:101172850])

HSP 1 Score: 83.1889 bits (204), Expect = 2.435e-16
Identity = 58/195 (29.74%), Postives = 86/195 (44.10%), Query Frame = 2 
Query:  101 GLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRK---LDGYGEN--GRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFL-HEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYN---SGQKDQPYEIGKPCSECSSSY-QFCRDDLCVPKSR 655
             +T  + +  LDLHN +R  VYPPAS+M+ + ++  +E+S  E+ E C +EH     L   G+N           T  V +W+ E   ++     E      N   PF      C HY  +V +    +GC    C N+   G  +  +    C Y+   +     PY+ G PCS C  SY   CRD+LC    R
Sbjct:   83 AITDSDAQLILDLHNKLRGQVYPPASNMEYMMWDTELERSAEEWAETCLWEHGPASLLPQIGQNLGAHWGRYQPPTFHVQAWYDEVKDYSFPYPQE-----CNPYCPFRCSGPVCTHYTQLVWATSSRIGCAINLCHNMDVWGQIWAKAVYLVCNYSPKGNWWGHAPYKHGTPCSACPPSYGGGCRDNLCYKDDR 272
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Medaka
Match: ENSORLT00000020676.2 (cysteine-rich venom protein pseudecin-like [Source:NCBI gene;Acc:101157728])

HSP 1 Score: 78.5666 bits (192), Expect = 3.773e-16
Identity = 53/189 (28.04%), Postives = 92/189 (48.68%), Query Frame = 2 
Query:  119 RKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH-----RKLDGY--GENGRLASDSNVTNTVM-SWFQERIYF---NISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFC-FYNSG--QKDQPYEIGKPCSECSSSYQFCRDDLCV 643
            + E +D+HN  RR+V PPA+DM  + Y+E +  S   ++++C  +H     R L+GY  GEN   ++   +  +++ +W++E  +F   N S  G+                  GHY  +V ++   +GCG   C N         + Y+  C +Y +G  ++  PY++G PC+ C +    C D LC 
Sbjct:   16 QAEIVDVHNAFRRAVEPPAADMLMMSYSEELAVSAQAWVDKCVLDHGPPSSRMLNGYELGENLFYSTSPMLWKSIINAWYKEVSHFAFPNASTNGK----------------PVGHYTQVVWNSSYKVGCGVTLCSN---------NIYLYGCHYYRAGNFKRWPPYKVGTPCASCPND---CVDKLCT 176
[Close]
BLAST of Peptidase inhibitor 16 vs. Ensembl Medaka
Match: crispld1b (cysteine-rich secretory protein LCCL domain-containing 1 [Source:NCBI gene;Acc:101169145])

HSP 1 Score: 82.4185 bits (202), Expect = 3.974e-16
Identity = 53/181 (29.28%), Postives = 83/181 (45.86%), Query Frame = 2 
Query:  128 FLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEH---RKLDGYGEN--GRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFL-HEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYN---SGQKDQPYEIGKPCSECSSSYQ-FCRDDLC 640
             +DLHN +R  VYPPAS+M+ + ++  +E+S   +   C++EH     L   G+N       D   T  V +W+ E  +++     E      N   P       C HY  +V +    +GC    C N+   G+ +  +    C Y+   +     PY+ G PCS C +SY   CRD+LC
Sbjct:   69 IVDLHNKLRGQVYPPASNMEYMMWDYELERSAEYWAHMCRWEHGPNHMLTQIGQNLGVHWGRDRPPTYHVQAWYDEVRHYSYPYSQE-----CNPHCPLRCSGPVCTHYTQLVWATSNRIGCAINVCYNMNVWGMIWAKAVYLVCNYSPPGNWWGHAPYKYGTPCSACPASYAGGCRDNLC 244
[Close]
BLAST of Peptidase inhibitor 16 vs. Planmine SMEST
Match: SMESG000066259.1 (SMESG000066259.1)

HSP 1 Score: 1242.25 bits (3213), Expect = 0.000e+0
Identity = 601/606 (99.17%), Postives = 602/606 (99.34%), Query Frame = 2 
Query:   47 MNFVFILAFVECALYQILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDKNSRTCKLKCRNCAEYFDPKNSCLCDCPDGTEGNSCRGTPAVVDENCPSCLAQGLSCNSGTEDSRSGNCNCKCTPEYIGEYCEIRKDQFDSSFYYVVDGTDWRYNMMIPVNAIEFKKSFYKFQNAIARLINEFCVKNLNSYPECKKIEENQIIYDNVLLIPGFPHLLENGSLAIRIFIIFNDVTDNVKQERFVSTTLLREILTNSDDIKNLIENVRKVVLIDNEIFNRSPFYAENDYQLNYEITKDKNMEYTPENTPNPTSFFEPISHTELLKTSTESPINTTVFMPTHFQESSYHHTKTSNSDHNSKETTKWKMSSEITVKTPTSTNVKPTDNDKTTTGPLTTSFQSFTNSNKLSTDDENSTSLLSTTFFPKTTTKCSNINKYASNGFFLLLMLYICGNL 1864
            MNFVFILAFVECALYQILGLTPRERKEFLDLHNHVRR VYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDKNSRTCKLKCRNCAEYFDPKNSCLCDCPDGTEGNSCRGTP VVDENCPSCLAQGLSCNSGTEDSRSGNCNCKC PEYIGEYCEIRKDQFDSSFYYVVDGTDWRYNMMIPVNAIEFKKSFYKFQNAIARLINEFCVKNLNSYPECKKIEENQIIYDNVLLIPGFPHLLENGSLAIRIFIIFNDVTDNVKQERFVST+LLREILTNSDDIKNLIENVRKVVLIDNEIFNRSPFYAENDYQLNYEITKDKNMEYTP NTPNPTSFFEPISHTELLKTSTESPINTTVFMPTHFQESSYHHTKTSNSDHNSKETTKWKMSSEITVKTPTSTNVKPTDNDKTTTGPLTTSFQSFTNSNKLSTDDENSTSLLSTTFFPKTTTKCSNINKYASNGFFLLLMLYICGNL
Sbjct:    1 MNFVFILAFVECALYQILGLTPRERKEFLDLHNHVRRLVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDKNSRTCKLKCRNCAEYFDPKNSCLCDCPDGTEGNSCRGTPVVVDENCPSCLAQGLSCNSGTEDSRSGNCNCKCPPEYIGEYCEIRKDQFDSSFYYVVDGTDWRYNMMIPVNAIEFKKSFYKFQNAIARLINEFCVKNLNSYPECKKIEENQIIYDNVLLIPGFPHLLENGSLAIRIFIIFNDVTDNVKQERFVSTSLLREILTNSDDIKNLIENVRKVVLIDNEIFNRSPFYAENDYQLNYEITKDKNMEYTPGNTPNPTSFFEPISHTELLKTSTESPINTTVFMPTHFQESSYHHTKTSNSDHNSKETTKWKMSSEITVKTPTSTNVKPTDNDKTTTGPLTTSFQSFTNSNKLSTDDENSTSLLSTTFFPKTTTKCSNINKYASNGFFLLLMLYICGNL 606
[Close]
BLAST of Peptidase inhibitor 16 vs. Planmine SMEST
Match: SMESG000031543.1 (SMESG000031543.1)

HSP 1 Score: 468.389 bits (1204), Expect = 2.608e-160
Identity = 229/418 (54.78%), Postives = 301/418 (72.01%), Query Frame = 2 
Query:   80 CALYQILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDKNSRTCKLKCRNCAEYFDPKNSCLCDCPDGTEGNSCRGTPAVVDENCPSCLAQGLSCNSGTEDSRSGNCNCKCTPEYIGEYCEIRKDQFDSSFYYVVDGTDWRYNMMIPVNAIEFKKSFYKFQNAIARLINEFCVKNLNSYPECKKIEENQIIYDNVLLIPGFPHLLENGSLAIRIFIIFNDVTDNVKQERFVSTTLLREILTN-SDDIKNLIENVRKVVLIDN 1330
            C +     LT +ERKEFLD+HN++RRSV+P ASDMQEL Y+ENIEK V EYLE+C FEHR+LD YGENGRL+SD+N+TN+V+SWFQERIYFNI   G+ RCSK N+ I +L + QCGHYKMMVKS LK +GCG+KFC +      +Y++ +++FCFY+SGQKDQPYEIG  CS+C ++YQFC DDLCVPK RYDK SR+CK+ C+NCA YFDP NSCLCDCP  T+GNSC+G   +   NC  C  +GL+C +G E+ +  NC+C C   + G+ CEI K QF +S+Y+V+DG  WR+ M IPVN +EFK S+ +FQ  I   I  +C K+  S  +C+KI+   +  +NVL I GFP LLENGSLA+RIF   +       +E  VS+ LL  +L N S +++ LI+N +++  I N
Sbjct:   13 CLIAFCFALTLKERKEFLDIHNYLRRSVFPTASDMQELIYDENIEKHVTEYLEKCVFEHRRLDNYGENGRLSSDNNITNSVVSWFQERIYFNIFETGDKRCSKKNTIIDYLPDSQCGHYKMMVKSTLKGIGCGHKFCTSATSGDRNYSNVFLTFCFYDSGQKDQPYEIGNICSDCDNAYQFCHDDLCVPKKRYDKISRSCKVVCKNCAPYFDPMNSCLCDCPLDTDGNSCKGNSTIA--NCSFCKKKGLACINGIENQKDKNCSCICEESFEGKQCEINKRQFSASYYHVIDGLPWRHGMNIPVNLLEFKNSYSEFQRFIHSDILNYC-KHKQSNEDCQKIKNIPLNSNNVLPIQGFPLLLENGSLALRIFFNLSVSLQASYKESLVSSALLGYVLNNPSKELEKLIDNSKRIYPIRN 427
[Close]
BLAST of Peptidase inhibitor 16 vs. Planmine SMEST
Match: SMESG000066258.1 (SMESG000066258.1)

HSP 1 Score: 470.315 bits (1209), Expect = 5.545e-159
Identity = 222/422 (52.61%), Postives = 301/422 (71.33%), Query Frame = 2 
Query:   53 FVFILAFVECALYQILGLTPRERKEFLDLHNHVRRSVYPPASDMQELEYNENIEKSVNEYLEQCKFEHRKLDGYGENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDKNSRTCKLKCRNCAEYFDPKNSCLCDCPDGTEGNSCRGTPAVVDENCPSCLAQGLSCNSGTEDSRSGNCNCKCTPEYIGEYCEIRKDQFDSSFYYVVDGTDWRYNMMIPVNAIEFKKSFYKFQNAIARLINEFCVKNLNSYPECKKIEENQIIYDNVLLIPGFPHLLENGSLAIRIFIIFNDVTDNVKQERFVSTTLLREILTN-SDDIKNLIENVRKV 1315
            F  +L F+     + L LTP ERK+FLD+HNH RR+V P AS+MQEL YNE IE  VN YLEQC+FEHRKLDG+GENGRLA+D N+T+TVMSWFQER+YFN    G  RCS+ NSK  +L +V+CGHYKMMV+  L SLGCGYK CK+  G+GI+Y +SYI+FCFYN+GQ D+PYEIGKPC+ C   Y FC  +LCVP  +Y +N  +C+LKC NCA +F  K SCLCDCP  TE   CRG   + + +C +C  + LSCN+G E S   NC CKCT  Y GEYC +R D+ ++S+++V+DGT WRYNM +P+N  +FK S+ KFQ  IA  I  FC+ + +S  +C +I    I  ++VLL+P  P +LENGSL+I +F+ ++ + +  KQE+FV   +L +I  + S ++++L+EN++++
Sbjct:    5 FKSLLLFLIVFPLKTLLLTPEERKQFLDIHNHFRRAVTPQASNMQELVYNETIENVVNGYLEQCRFEHRKLDGFGENGRLATDKNITDTVMSWFQERVYFNYFKNGSSRCSQENSKFEYLSKVECGHYKMMVRGTLASLGCGYKLCKSPYGDGITYPESYITFCFYNAGQMDRPYEIGKPCTGCEKGYLFCYRELCVPADKYFRNQTSCELKCLNCATFFS-KKSCLCDCPSNTEAEVCRGN--INNPSCTTCQKKALSCNNGEEVSVDNNCTCKCTKAYQGEYCNVRIDKHEASYHHVIDGTKWRYNMSVPINIFQFKNSYEKFQTVIALDIFNFCLTS-SSTKQCSEIVTYSINQNDVLLLPESPIILENGSLSIEMFVNYSIMNEIGKQEKFVPPFILSQIWNSPSPNLQSLVENIKRI 422
[Close]
BLAST of Peptidase inhibitor 16 vs. Planmine SMEST
Match: SMESG000031543.1 (SMESG000031543.1)

HSP 1 Score: 294.278 bits (752), Expect = 1.925e-94
Identity = 148/297 (49.83%), Postives = 202/297 (68.01%), Query Frame = 2 
Query:  443 MVKSNLKSLGCGYKFCKNIQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVPKSRYDKNSRTCKLKCRNCAEYFDPKNSCLCDCPDGTEGNSCRGTPAVVDENCPSCLAQGLSCNSGTEDSRSGNCNCKCTPEYIGEYCEIRKDQFDSSFYYVVDGTDWRYNMMIPVNAIEFKKSFYKFQNAIARLINEFCVKNLNSYPECKKIEENQIIYDNVLLIPGFPHLLENGSLAIRIFIIFNDVTDNVKQERFVSTTLLREILTN-SDDIKNLIENVRKVVLIDN 1330
            MVKS LK +GCG+KFC +      +Y++ +++FCFY+SGQKDQPYEIG  CS+C ++YQFC DDLCVPK RYDK SR+CK+ C+NCA YFDP NSCLCDCP  T+GNSC+G   +   NC  C  +GL+C +G E+ +  NC+C C   + G+ CEI K QF +S+Y+V+DG  WR+ M IPVN +EFK S+ +FQ  I   I  +C K+  S  +C+KI+   +  +NVL I GFP LLENGSLA+RIF   +       +E  VS+ LL  +L N S +++ LI+N +++  I N
Sbjct:    1 MVKSTLKGIGCGHKFCTSATSGDRNYSNVFLTFCFYDSGQKDQPYEIGNICSDCDNAYQFCHDDLCVPKKRYDKISRSCKVVCKNCAPYFDPMNSCLCDCPLDTDGNSCKGNSTIA--NCSFCKKKGLACINGIENQKDKNCSCICEESFEGKQCEINKRQFSASYYHVIDGLPWRHGMNIPVNLLEFKNSYSEFQRFIHSDILNYC-KHKQSNEDCQKIKNIPLNSNNVLPIQGFPLLLENGSLALRIFFNLSVSLQASYKESLVSSALLGYVLNNPSKELEKLIDNSKRIYPIRN 294
[Close]
BLAST of Peptidase inhibitor 16 vs. Planmine SMEST
Match: SMESG000055797.1 (SMESG000055797.1)

HSP 1 Score: 88.9669 bits (219), Expect = 5.343e-20
Identity = 69/222 (31.08%), Postives = 100/222 (45.05%), Query Frame = 2 
Query:   38 QLAMNFVFILAFVECALYQILG-LTPRERKEFLDLHNHVRRSVY-------PPASDMQELEYNENIEKSVNEYLEQCKFEH-----RKLDGY---GENGRLASDSNVTNTVMSWFQERIYFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKNIQGNGISYTDSYISF--CFYNSG---QKDQPYEIGKPCSECSSSYQFCRDDLC 640
            Q+ MNF+  + FV  AL    G L+  ER + L++HN  R+SV        P A++MQ++ ++E          ++C F H     RK   Y   G+N  + +  N+   V  WF E   +  S   +  CS          E  CGHY  +V +  K LGCG   CK        Y+   ++F  C Y  G     + PYE G+PCS+C S    C + LC
Sbjct:    3 QITMNFIIKITFVLVALDFCSGDLSEEERNQILEIHNKFRKSVANGEVSNQPKAANMQQMVWSEEAASRAQATADKCSFNHDTINDRKYGNYEWVGQNIAMFTGGNIAKGVQLWFNEVSGYTFS---DNSCS----------ENTCGHYTQIVWAKSKELGCGLAKCK--------YSKYKMNFLVCNYGPGGNFNSELPYETGEPCSKCQSGSN-CSNGLC 202
The following BLAST results are available for this feature:
BLAST of Peptidase inhibitor 16 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PI163.279e-2032.11peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
PI163.279e-2032.11peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
PI163.279e-2032.11peptidase inhibitor 16 [Source:HGNC Symbol;Acc:HGN... [more]
R3HDML8.742e-1928.49R3H domain containing like [Source:HGNC Symbol;Acc... [more]
PI158.716e-1627.57peptidase inhibitor 15 [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of Peptidase inhibitor 16 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
lon-12.040e-1027.87pep chromosome:WBcel235:III:5474478:5477999:1 gene... [more]
lon-12.752e-1029.03pep chromosome:WBcel235:III:5474478:5477999:1 gene... [more]
lon-17.366e-927.00pep chromosome:WBcel235:III:5474478:5477999:1 gene... [more]
lon-17.366e-927.00pep chromosome:WBcel235:III:5474478:5478005:1 gene... [more]
scl-19.941e-826.43SCP-Like extracellular protein [Source:UniProtKB/... [more]
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BLAST of Peptidase inhibitor 16 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG84839.369e-726.37gene:FBgn0038126 transcript:FBtr0082782[more]
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BLAST of Peptidase inhibitor 16 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
r3hdml2.169e-2031.66R3H domain containing-like [Source:ZFIN;Acc:ZDB-GE... [more]
r3hdml2.169e-2031.66R3H domain containing-like [Source:ZFIN;Acc:ZDB-GE... [more]
crispld22.194e-1629.53cysteine-rich secretory protein LCCL domain contai... [more]
crispld1a2.517e-1629.25cysteine-rich secretory protein LCCL domain contai... [more]
crispld1a5.029e-1528.05cysteine-rich secretory protein LCCL domain contai... [more]
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BLAST of Peptidase inhibitor 16 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
R3HDML7.757e-2128.06R3H domain containing-like [Source:NCBI gene;Acc:1... [more]
R3HDML1.037e-2028.06R3H domain containing-like [Source:NCBI gene;Acc:1... [more]
ENSXETT00000036788.17.635e-1832.09pep primary_assembly:Xenopus_tropicalis_v9.1:KV464... [more]
CRISPLD14.402e-1530.21cysteine-rich secretory protein LCCL domain contai... [more]
R3HDML1.072e-1425.89R3H domain containing-like [Source:NCBI gene;Acc:1... [more]
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BLAST of Peptidase inhibitor 16 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pi164.233e-2130.50peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
Pi164.233e-2130.50peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
Pi161.199e-2029.25peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
R3hdml1.602e-2029.47R3H domain containing-like [Source:MGI Symbol;Acc:... [more]
Pi168.877e-2030.50peptidase inhibitor 16 [Source:MGI Symbol;Acc:MGI:... [more]
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BLAST of Peptidase inhibitor 16 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A2A5I3|CRSPL_MOUSE1.121e-1929.47Peptidase inhibitor R3HDML OS=Mus musculus OX=1009... [more]
sp|Q6UXB8|PI16_HUMAN1.574e-1932.11Peptidase inhibitor 16 OS=Homo sapiens OX=9606 GN=... [more]
sp|Q9ET66|PI16_MOUSE6.210e-1930.50Peptidase inhibitor 16 OS=Mus musculus OX=10090 GN... [more]
sp|Q9H3Y0|CRSPL_HUMAN4.198e-1828.49Peptidase inhibitor R3HDML OS=Homo sapiens OX=9606... [more]
sp|Q58D34|PI16_BOVIN1.110e-1730.81Peptidase inhibitor 16 OS=Bos taurus OX=9913 GN=PI... [more]
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BLAST of Peptidase inhibitor 16 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8HLS14.075e-3029.61SCP domain-containing protein OS=Macrostomum ligna... [more]
A0A1I8GC847.063e-3026.35SCP domain-containing protein OS=Macrostomum ligna... [more]
A0A267DUN51.016e-2926.35SCP domain-containing protein (Fragment) OS=Macros... [more]
A0A267H7011.491e-2825.00Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FXP21.501e-2825.00Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of Peptidase inhibitor 16 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000036030.14.805e-1827.40peptidase inhibitor 16 [Source:NCBI gene;Acc:10302... [more]
ENSAMXT00000029824.19.262e-1827.57peptidase inhibitor 16 [Source:NCBI gene;Acc:10302... [more]
ENSAMXT00000031557.19.724e-1827.57peptidase inhibitor 16 [Source:NCBI gene;Acc:10302... [more]
r3hdml2.271e-1728.49R3H domain containing like [Source:NCBI gene;Acc:1... [more]
crispld1b2.717e-1730.00cysteine rich secretory protein LCCL domain contai... [more]
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BLAST of Peptidase inhibitor 16 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSPMAT00000000374.16.411e-1527.27pep scaffold:Pmarinus_7.0:GL476650:75849:87118:-1 ... [more]
ENSPMAT00000002250.13.693e-1025.57pep scaffold:Pmarinus_7.0:GL477299:1626:22672:-1 g... [more]
CRISP33.452e-823.38cysteine rich secretory protein 3 [Source:HGNC Sym... [more]
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BLAST of Peptidase inhibitor 16 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase inhibitor 16 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Peptidase inhibitor 16 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000020674.21.306e-1627.72cysteine-rich venom protein pseudecin-like [Source... [more]
crispld1a1.949e-1629.74cysteine rich secretory protein LCCL domain contai... [more]
crispld1a2.435e-1629.74cysteine rich secretory protein LCCL domain contai... [more]
ENSORLT00000020676.23.773e-1628.04cysteine-rich venom protein pseudecin-like [Source... [more]
crispld1b3.974e-1629.28cysteine-rich secretory protein LCCL domain-contai... [more]
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BLAST of Peptidase inhibitor 16 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000066259.10.000e+099.17SMESG000066259.1[more]
SMESG000031543.12.608e-16054.78SMESG000031543.1[more]
SMESG000066258.15.545e-15952.61SMESG000066258.1[more]
SMESG000031543.11.925e-9449.83SMESG000031543.1[more]
SMESG000055797.15.343e-2031.08SMESG000055797.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002471 ID=SMED30002471|Name=Peptidase inhibitor 16|organism=Schmidtea mediterranea sexual|type=transcript|length=1929bp
AGTTTATTCGCTATAATAAAAATAAAGTTATTGAAAACAATTAGCAATGA
ATTTTGTATTTATTTTGGCATTTGTCGAGTGCGCTTTGTATCAAATCCTA
GGATTGACCCCCCGAGAAAGAAAAGAATTTCTTGACTTACACAATCATGT
CAGAAGGTCGGTTTATCCGCCGGCCTCTGACATGCAGGAGCTGGAATATA
ACGAAAATATAGAAAAGTCAGTAAATGAATATTTAGAACAATGCAAATTT
GAGCACAGAAAATTGGACGGATATGGAGAAAATGGCCGACTGGCCAGCGA
CAGCAACGTAACCAATACTGTAATGAGTTGGTTTCAAGAAAGAATTTATT
TCAATATTTCGGCCGAAGGTGAAGGCCGTTGTTCAAAATCCAACAGTAAA
ATTCCATTTCTTCACGAGGTTCAGTGTGGTCATTACAAAATGATGGTTAA
AAGTAATTTAAAATCACTTGGATGTGGTTACAAATTTTGCAAAAATATTC
AAGGAAACGGGATTAGTTACACAGATTCTTATATTAGTTTTTGTTTTTAT
AACTCAGGACAGAAAGATCAGCCTTATGAAATTGGAAAACCTTGCAGTGA
ATGCAGTTCAAGCTATCAATTTTGTCGAGATGATCTCTGCGTCCCAAAAT
CAAGATATGATAAAAATTCAAGGACGTGCAAACTGAAATGCAGGAATTGT
GCTGAATACTTTGATCCAAAAAATTCTTGTCTATGTGATTGTCCAGATGG
AACCGAAGGAAATTCTTGCAGAGGGACTCCTGCGGTTGTTGATGAAAATT
GTCCATCATGTCTTGCACAAGGTTTATCATGTAATTCCGGTACTGAAGAT
TCGAGGAGCGGAAATTGCAATTGCAAATGCACACCTGAATACATTGGTGA
ATATTGTGAAATCAGAAAAGATCAATTTGATTCCTCCTTTTATTATGTTG
TTGATGGAACAGACTGGCGTTATAATATGATGATTCCTGTGAATGCTATA
GAATTTAAAAAATCTTTTTACAAATTTCAAAATGCCATCGCCCGACTTAT
CAATGAGTTTTGTGTAAAAAATTTGAATTCTTATCCAGAGTGCAAAAAGA
TTGAAGAAAACCAAATTATTTATGATAATGTATTACTTATTCCGGGCTTT
CCACATTTGCTTGAAAACGGTTCATTGGCAATAAGAATTTTCATCATTTT
CAATGACGTCACCGATAATGTAAAGCAAGAGAGATTCGTTTCCACTACTT
TATTGCGAGAAATTCTGACAAATTCAGACGATATTAAAAACTTAATTGAA
AATGTGAGAAAGGTTGTCCTAATTGACAATGAAATATTCAATCGAAGTCC
ATTTTATGCAGAGAATGATTACCAACTAAATTATGAAATAACAAAAGATA
AAAACATGGAGTATACGCCTGAAAATACACCTAACCCTACTAGCTTTTTT
GAGCCAATTTCACATACTGAATTATTAAAGACTTCAACTGAAAGCCCGAT
TAATACCACTGTCTTTATGCCGACTCATTTTCAGGAGTCAAGTTACCATC
ATACTAAGACTTCAAATTCTGATCACAATTCCAAAGAAACAACAAAATGG
AAAATGAGTTCAGAAATTACAGTCAAAACACCAACATCAACAAACGTCAA
ACCGACAGATAATGACAAAACAACCACCGGTCCATTGACAACATCCTTCC
AAAGTTTTACCAATTCAAACAAATTGTCTACAGATGATGAAAATTCAACA
TCATTGTTGTCAACAACGTTTTTTCCAAAAACAACAACAAAATGTTCAAA
CATCAACAAATATGCGTCAAATGGCTTCTTTTTGCTTTTAATGTTGTACA
TTTGTGGGAATTTATGAAATCAAAAAAATGGTTTTGACTGAAAATTGTTT
TAATTAACTAAAATTAAAATTAGAATACA
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protein sequence of SMED30002471-orf-1

>SMED30002471-orf-1 ID=SMED30002471-orf-1|Name=SMED30002471-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=607bp
MNFVFILAFVECALYQILGLTPRERKEFLDLHNHVRRSVYPPASDMQELE
YNENIEKSVNEYLEQCKFEHRKLDGYGENGRLASDSNVTNTVMSWFQERI
YFNISAEGEGRCSKSNSKIPFLHEVQCGHYKMMVKSNLKSLGCGYKFCKN
IQGNGISYTDSYISFCFYNSGQKDQPYEIGKPCSECSSSYQFCRDDLCVP
KSRYDKNSRTCKLKCRNCAEYFDPKNSCLCDCPDGTEGNSCRGTPAVVDE
NCPSCLAQGLSCNSGTEDSRSGNCNCKCTPEYIGEYCEIRKDQFDSSFYY
VVDGTDWRYNMMIPVNAIEFKKSFYKFQNAIARLINEFCVKNLNSYPECK
KIEENQIIYDNVLLIPGFPHLLENGSLAIRIFIIFNDVTDNVKQERFVST
TLLREILTNSDDIKNLIENVRKVVLIDNEIFNRSPFYAENDYQLNYEITK
DKNMEYTPENTPNPTSFFEPISHTELLKTSTESPINTTVFMPTHFQESSY
HHTKTSNSDHNSKETTKWKMSSEITVKTPTSTNVKPTDNDKTTTGPLTTS
FQSFTNSNKLSTDDENSTSLLSTTFFPKTTTKCSNINKYASNGFFLLLML
YICGNL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000420parapharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR035940CAP_sf
IPR001283CRISP-related
IPR014044CAP_domain
IPR011528NERD
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014044CAP domainSMARTSM00198SCP_3coord: 23..176
e-value: 2.1E-10
score: 50.6
IPR014044CAP domainPFAMPF00188CAPcoord: 29..153
e-value: 5.1E-16
score: 59.7
IPR035940CAP superfamilyGENE3DG3DSA:3.40.33.10coord: 20..212
e-value: 1.7E-35
score: 124.3
IPR035940CAP superfamilySUPERFAMILYSSF55797PR-1-likecoord: 10..179
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 499..520
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 503..517
NoneNo IPR availablePANTHERPTHR10334:SF62PEPTIDASE INHIBITOR R3HDMLcoord: 22..202
NoneNo IPR availableCDDcd05559CAP_PI16_HrTT-1coord: 25..168
e-value: 1.21487E-23
score: 94.7918
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.613
IPR001283Cysteine-rich secretory protein-relatedPANTHERPTHR10334CYSTEINE-RICH SECRETORY PROTEIN-RELATEDcoord: 22..202