SMED30002449

Overview
NameSMED30002449
Smed IDSMED30002449
Length (bp)2041
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002449 (SMED30002449) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002449 (SMED30002449) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002449 (SMED30002449) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002449 (SMED30002449) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002449

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30002449h1SMcG0009850 dd_Smed_v4_20805_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30002449h1SMcG0009850 dd_Smed_v4_20805_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30002449h1SMcG0009850 dd_Smed_v4_20805_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30002449h1SMcG0009850 dd_Smed_v4_20805_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30002449h1SMcG0009850 dd_Smed_v6_20805_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30002449 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.005126:4905..14172 -9455
Homology
[Close]
BLAST of SMED30002449 vs. TrEMBL
Match: R7UDU4 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_207377 PE=4 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 5.431e-27
Identity = 80/341 (23.46%), Postives = 149/341 (43.70%), Query Frame = 3 
Query: 1002 FSLEEITRLKLITRSTFGEIALSLLSSGIKHHHHIITKSVNIKDYP------CFITVDMSSNSQR-KCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFMRSYYRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAIKYQVAQLSKLLENRKVPGEKS-NSINLKNIKFGVDQSFPTSSLTDNQILNLLNDIQINMQN--------------EKNHSI--EEIEEMKLNFSVLFSALKEKKKNIGKFIEFEEDIENSSNSVNRRFSLPA 1952
            FSL   TR+K +TRSTF ++ L++ +  I+       K++  +  P      C + VD+ S+S   K G+Q      T  DI LP +TEG IP LW++K +M  LK+S+D +  +     ++ +    +  +   +   KS   +S+L G    +     +   +++W P    + +S+   +Y+DQ  + +    +   N +L+      ++ +L  LL +R+ PGE     + L   + G  Q       +  +I   + ++Q+ +Q               E N S     +E++K  F  L   L+ K+ + G  +   +D        +     PA
Sbjct:  467 FSLPCATRIKQVTRSTFNDVLLAVAAGNIR-------KALQAQGMPNPYDMLCTMPVDLRSDSSPIKMGVQ-----FTLNDISLPTNTEGTIPRLWEVKHRMDELKNSADPVIVHESVNVLANILPAFIWTKIHSAIINKSSCFISNLQGPDYSLTFASRRMKRIIYWMPTKGEVGLSISFLTYSDQLQMAVIADRALVPNPELITQDFTKEMEKLKNLLAHRRTPGEHPMRRMELAIKEDGYSQG---DGCSIEEIQKKICEVQMQLQEVKAELDVDGPTEDYEANRSALNNRVEQLKEEFRELLIELRRKRASDGTLL--SDDARGRGGRRDEALVPPA 790

HSP 2 Score: 54.299 bits (129), Expect = 5.431e-27
Identity = 46/221 (20.81%), Postives = 94/221 (42.53%), Query Frame = 1 
Query:  331 VLFMDKPVELSELKRVISERIGNLDSSSNKN-YQITKRLRQTAQKIDFHWRWVEECEFDLCHHVTLLTPKFTELSDI---LVEISNKPYDFSHPFWNLYLINSPISSNQGKSCSSTIKINEKKCKLCE----SNSTIVLKIHPSITDGMCIMKLLSEIFNDMPEPSQLGINNYRK------KFCFKAFTALFYSFYIFIKSWSFRQAQYRLLIVPIISLHR 951
            +  +D  ++++ +K ++  RI    S+ NKN +++  R  Q    +   + W  + +F + +HV  + P     SD+   +  ++++      P W L++                         LC+     ++  +L++HP ++DG+ +M++L     D     Q   NN  K       F F    A+F    +F++ W F +  + LL  P +S H+
Sbjct:  272 LFVLDSGLDVTRIKDLVHSRIV---SAENKNRHKVYPRFTQKLVPLYTGYAWENDIDFHMDNHVFAVPPTVRTESDLQEYIALMASQSLPNDKPLWELHV-------------------------LCDYGHLKDTACLLRVHPCMSDGISLMQILLRSLVD-----QTTSNNVIKPRFGQGAFIFNFIRAIFVGVLVFLQKWLFTRGDFNLLRGPHLSGHK 459
[Close]
BLAST of SMED30002449 vs. TrEMBL
Match: A0A210Q8G4 (Diacylglycerol O-acyltransferase OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT07826 PE=4 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.623e-26
Identity = 65/227 (28.63%), Postives = 113/227 (49.78%), Query Frame = 3 
Query: 1002 FSLEEITRLKLITRSTFGEIALSLLSSGIKHHHHIITKSV-NIKDYPCFITVDMSSNSQRKCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFM----RSYYRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAIKYQVAQLSKLLENRKVPGEK 1667
            +SLE+ TRL  +TRST  +I LS+ +  ++++  + T  + N  D    + V   S       +    ND  +  + +P +TEG IP LW++K  M  + S    L F+ + +   +L ST L +R++    RS Y K   ++S L G    + I   K  +++ W P    ++V++  F+Y DQ  + +    +   N ++L       +  LS LL NR++PGE+
Sbjct:  632 YSLEQATRLSQVTRSTLNDIFLSVTAGCLRNY--LQTNGIPNPYDMQALVPVHYHSGPS---PLHLQNNDCLFIPVSIPSNTEGAIPRLWRVKHIMDKIGS----LAFFSVTQGAHMLSSTILPQRWLYSMWRSLYSKCTFVISDLPGPETTLKIATKKIKSVICWMPPIHQVAVAISFFTYADQVRMSVMADRTVLPNPEMLTRDFIAIMDDLSSLLGNRRIPGEQ 849

HSP 2 Score: 53.9138 bits (128), Expect = 1.623e-26
Identity = 48/229 (20.96%), Postives = 102/229 (44.54%), Query Frame = 1 
Query:  268 GMSLTFEEARWLSEMSASMIG---VLFMDKPVELSELKRVISER-IGNLDSSSNKNY-QITKRLRQTAQKIDFHWRWVEECEFDLCHHVTLLTPKFTELSDI---LVEISNKPYDFSHPFWNLYLINSPISSNQGKSCSSTIKINEKKCKLCESNSTIVLKIHPSITDGMCIMKLLSEIFNDMPEPSQLGINNY--RKKFCFKAFTALFYSFYIFIKSWSFRQAQYRLL 924
            G  L+ +E  W   +  S +    V+ ++  +   +++ ++  R I   D + N+ Y + TK++  T         W+++  F + +HV  +      + D+   + E S+KP    HP W + ++                          + ++ ++L+IHP I DG+ +++LL +   DM E   + +  +  R++FC  A  A F    +F+K + F++  + +L
Sbjct:  412 GTLLSPKERAWAENLETSTVVYQCVVIVEYGLTTYKIRELVRNRLIKATDKNGNRLYPKFTKKIVNTFSGPS----WIQDEAFLINNHVFNMPSSIENMEDLQEYISESSSKPLSMHHPLWEIQVLTDFAD---------------------QRDTVVLLRIHPCIADGISLVQLLYKAIVDM-ESITIAMPKFSSRRRFC-NAVQAFFSGPLVFLKQYVFKKQDFNML 613
[Close]
BLAST of SMED30002449 vs. TrEMBL
Match: A0A0L8I925 (Uncharacterized protein (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22027692mg PE=4 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 9.328e-25
Identity = 60/224 (26.79%), Postives = 108/224 (48.21%), Query Frame = 3 
Query: 1002 FSLEEITRLKLITRSTFGEIALSLLSSGIKHH--HHIITKSVNIKDYPCFITVDMSSNSQRKCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFMRSYYRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAIKYQVAQLSKLLENRKVPGEK 1667
            FSL   TR+K I R T  ++ +SL++ G++ +   + I    N+K   C I V+M   +        + N   + +  +P +TEG IP LW +K  M   K SS+YL         S +   S   R   +       L+S++ G    + ++     ++++W P  + + +++  F+Y +Q  + + G  S   N + L     +Q+ QLS L+ +R+VPGE+
Sbjct:  442 FSLSVATRVKQIARCTLNDLFMSLVAGGLRSYLQQNGIENPFNLK---CIIPVNMQVRTN------NMSNHYVFINSTIPTNTEGAIPRLWHVKCSMAKFKKSSEYLVLNGAMWLSSCMLPESWHDRVWSTINNHCTCLISNMAGPENVLTLDTKVIRSIMYWMPTPDQVPIAISFFTYAEQIRMAVIGDRSVLPNPEFLTKYFVFQLRQLSNLVAHRRVPGEQ 656

HSP 2 Score: 48.1358 bits (113), Expect = 9.328e-25
Identity = 47/223 (21.08%), Postives = 97/223 (43.50%), Query Frame = 1 
Query:  277 LTFEEARWLSEM---SASMIGVLFMDKPVELSELKRVISERIGNLDSSSNKNYQITKRLRQTAQKIDFHWRWVEECEFDLCHHVTLLTP---KFTELSDILVEISNKPYDFSHPFWNLYLINSPISSNQGKSCSSTIKINEKKCKLCESNSTIVLKIHPSITDGMCIMKLLSEIFNDMPEPSQLGINNYRK-KFCFKAFTALFYSFYIFIKSWSFRQAQYRLL 924
            LT  E  WL      SA +  +L ++  ++   +K ++  R+  + +   +N     RL Q        + WV + +F+L +H+  L+       EL D + E+++K  + +HP W + +I+     N G S                 ++ I+ +IHP ++D   ++++  + F D  +P+     +  K + C      +F    +F++ W   ++   LL
Sbjct:  225 LTTHEKMWLRSPPLNSAVIQCLLVVEHGLDTPTIKDLLYTRL--IIAEKRRNQATFPRLVQKVVPFCTGYAWVYDEDFNLENHIHTLSDFIHTEEELQDFVSEMASKELNMNHPLWEVQIIH-----NFGPS----------------RDTLILFRIHPCMSDSTSLVRVFLKAFVDN-QPNLSPTRHLPKGRMCMNYIGGIFTGPLVFLQKWLCTKSDLNLL 423
[Close]
BLAST of SMED30002449 vs. TrEMBL
Match: A0A147BBB6 (Putative wax ester synthase-like acyl-coa acyltransferase domain protein (Fragment) OS=Ixodes ricinus OX=34613 PE=4 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 2.072e-23
Identity = 56/221 (25.34%), Postives = 106/221 (47.96%), Query Frame = 3 
Query: 1008 LEEITRLKLITRSTFGEIALSLLSSGIKHHHHIITKS--VNIKDYPCFITVDMSSNSQRKCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFMRSYYRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAIKYQVAQLSKLLENRKVPGE 1664
            + ++ R+K ITRSTF ++ ++ +S  +++      K   VN  D    + VD+           ++   L    + LP +TEG IP LW+++Q M+ LKSS D +  Y   + +  L    L+  F+  + RK   +L ++ G    +++  ++  +++ W      + V++   SY   F++ +  Q     +   +V     Q+  LS LL  R++PGE
Sbjct:  217 MSQVHRIKQITRSTFNDVLMTAMSGSLRN---FFKKRGIVNPPDIKVCLAVDLRYEPASGEPTPELGTQLATALVRLPTNTEGAIPRLWEVRQVMEELKSSPDPVVMYGASQLLFTLLPYRLAHWFLGLWQRKPSAVLCNVPGPENHVVLGLSRVRSVVAWAGWTPDVPVAVTVTSYAGAFSVAVSAQVDVVPDPDEIVRDFARQLNHLSDLLSKRRIPGE 434

HSP 2 Score: 47.7506 bits (112), Expect = 2.072e-23
Identity = 43/192 (22.40%), Postives = 85/192 (44.27%), Query Frame = 1 
Query:  331 VLFMDKPVELSELKRVISERIGNLDSSSNKNYQITKRLRQTAQKIDFHWRWVEECEFDLCHHVTLLTPKFT---ELSDILVEISNKPYDFSHPFWNLYLINSPISSNQGKSCSSTIKINEKKCKLCESNSTIVLKIHPSITDGMCIMKLLSEIFNDMPEPS-QLGINNYRKKFCFKAFTALFYSFYIFIKSW 894
            V F++  +  S L ++I+ERI  LD ++++  +I +R  +    +   + WVE+  F + HHV   T       +L   L+ + ++  + + P W+L+++    + ++G+                   + +V ++H  I+DG+ +M L      D P P  +L        F    F AL      F+ +W
Sbjct:   18 VFFVEPGLTASLLGQLIAERI--LDKTNDEGKRIFRRFTRKVVPVVGGFTWVEDDRFRIEHHVLEDTRSLKDSHQLRHYLMALMSRGMNVNRPLWDLHVLP---NFDKGR------------------ETVLVARVHQVISDGVSLMMLFCNHLCD-PGPGLRLKPRFGGSSFPLNVFRALVVGPLTFLVTW 185
[Close]
BLAST of SMED30002449 vs. TrEMBL
Match: V4AUH4 (DUF1298 domain-containing protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_239021 PE=4 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 8.363e-23
Identity = 82/324 (25.31%), Postives = 150/324 (46.30%), Query Frame = 3 
Query: 1002 FSLEEITRLKLITRSTFGEIALSLLSSGIKHHHHI--ITKSVNIKDYPCFITVDMSSNSQRKCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFMRSYYRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAIKYQVAQLSKLLENRKVPGE----KSNSINLKNI----KFGVDQSFPTSSLTDNQILNLLNDIQINMQNEKNHS----IEEIEEMKLNFSVLFSALKEKKKN-------IGKFIEFEEDIE 1910
            FSL   TR+K + R T  E+ +S++++ ++ +  I  I+   N+    C +  D ++N        K+ N  T+  + LP +TEG IP LW+ K  M+  K S +         F   +   S  + F R  YRK   ++S+L G    + +   +   +++W P  E I+VS+   +Y +Q  + +    S   N +LL      Q+  LS+LL NR++PGE    K+++  L +        +DQ     +L   +I ++   +  +  +  +HS    ++ IE++K  F  +   L+ K+         I   +  EE+I+
Sbjct:  118 FSLSAATRIKQVARCTLNELFISVVAANLRQYMQINGISNPFNMH---CAVPTDFNTNQSNI----KMGNKYTFVILPLPTNTEGAIPRLWETKISMENFKLSPEAAVVRGALWFTHAILPKSSCRNFWRRIYRKCSCVISNLVGPKTVLKLANREIKCIIYWLPPLEEIAVSISFITYGEQIRMAVVSDRSVLPNPELLTADFYQQLETLSQLLANRRIPGEQLHRKTDNTQLLSAFSIKDLSIDQMQLQMTLLQQEIHDIKTQLDASSSHRISHSEAHLMQRIEQLKEQFREIMVELRRKRSEENEAAVVISDDVSLEEEID 434

HSP 2 Score: 32.3426 bits (72), Expect = 8.363e-23
Identity = 19/68 (27.94%), Postives = 35/68 (51.47%), Query Frame = 1 
Query:  700 ESNSTIVL-KIHPSITDGMCIMKLLSEIFND--MPEPSQLGINNYRKKFCFKAFTALFYSFYIFIKSW 894
            ES  T++L ++HP +TDG+ ++++L +   D  M  P  L         C ++F A F     F++ +
Sbjct:   23 ESLDTVLLFRMHPCMTDGISLVRILEQALVDTHMITPHDLNFAGTMNT-CLQSFKAFFLGPITFLQKY 89
[Close]
BLAST of SMED30002449 vs. Ensembl Nematostella
Match: EDO30538 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T0H6])

HSP 1 Score: 63.5438 bits (153), Expect = 1.619e-10
Identity = 45/214 (21.03%), Postives = 97/214 (45.33%), Query Frame = 3 
Query: 1017 ITRLKLITRSTFGEIALSLLSSGIKHHHHIITKSVNIKDYPCFITVDMSSNSQRKCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFMRSYYRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAIKYQVAQLSKLLENRKVP 1658
            I ++K ITR+    + +S L+  ++   +     V      C  T   + +S+      K+ +  +   ++LP  TEG +P LW+ +++M T  S  +    +   KF+ ++   S ++RF+     KS   ++ + G   P+ +N   A  +    P      +S+   ++ +Q  + I        + ++L++  + ++ +L K L NR +P
Sbjct:  333 IKKIKDITRTKVDIVLMSCLAGALRSFLYKCGDEVPADVRACVQTDIRAQHSK-----LKLDSKFSAMFVDLPSGTEGAVPRLWETRRRMDTFSSVIERHALHAFLKFVLLILPISFARRFVDYTAGKSSCTVTYIAGPNTPVYLNGKMAKLMTILSPRITGQGLSLSLVTHANQVRLGIVAHQRLPCDPKILLNDFQREIDELHKHLSNRTLP 541
[Close]
BLAST of SMED30002449 vs. Ensembl Nematostella
Match: EDO40204 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S7Y9])

HSP 1 Score: 51.9878 bits (123), Expect = 6.900e-7
Identity = 43/199 (21.61%), Postives = 89/199 (44.72%), Query Frame = 3 
Query: 1002 FSLEEITRLKLITRSTFGEIALSLLSSGIKHHHHIITKSVN-IKDYPCFITVDMSSNSQRKCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFMRSY---------YRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQN 1568
            F L  + ++K  T +T  ++ ++ LS  I+ +     + +N  +D+   I VD+   ++        +N      ++LP+S  G++  L++ K++M  LK S +          I++ +S ++S  F+  +           K+  +LS++ G    + ++ N    L FW P    I + +  FSY  Q  +   G +
Sbjct:  339 FDLNIVKQIKSKTGTTVNDVLMACLSLAIRRYFQ--KRGINNPEDFTSSIPVDVGKPTKETV----FQNKFAVVFMKLPVSHNGILETLFETKRRMDVLKMSGEP---------IAMAYSMAMSTEFLPEFLVKPLASFIASKASCVLSNVPGPQYHMSVSGNPVQALTFWPPQRSNIGIGLSIFSYAGQVVVGADGDH 522
[Close]
BLAST of SMED30002449 vs. Planmine SMEST
Match: SMESG000051066.1 (SMESG000051066.1)

HSP 1 Score: 747.658 bits (1929), Expect = 0.000e+0
Identity = 377/403 (93.55%), Postives = 381/403 (94.54%), Query Frame = 3 
Query:  828 KKVLFQSVHCTFLFVLYFHQIMVF*TSTISFVDSSDYKSPSEIPSQLEINSHQFVFCHFSLEEITRLKLITRSTFGEIALSLLSSGIKHHHHIITKSVNIKDYPCFITVDMSSNSQRKCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFMRSYYRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAIKYQVAQLSKLLENRKVPGEKSNSINLKNIKFGVDQSFPTSSLTDNQILNLLNDIQINMQNEKNHSIEEIEEMKLNFSVLFSALKEKKKNIGKFIEFEEDIENSSNSVNRRFSLPAMTKVLQKNSSKRFSFSKQQIKITDNNEN 2036
            KK  F++    F     F +   F  +     DSSDYKSPSEIPSQLEINSHQFVFCHFSLEEITRLKLITRSTFGEIALSLLSSGIKHHHHIITKSVNIKDYPCFITVDMSSNSQRKCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFMRSYYRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAIKYQVAQLSKLLENRKVPGEKSNSINLKNIKFGVDQSFPTSSLTDNQILNLLNDIQINMQNEKNHSIEEIEEMKLNFSVLFSALKEKKKNIGKFIEFEEDIENSSNSVNRRFSLPAMTKVLQKNSSKRFSFSKQQIKITDNNEN
Sbjct:  271 KKFCFKAFTALFYSFYIFIKSWSFRQAQYRLFDSSDYKSPSEIPSQLEINSHQFVFCHFSLEEITRLKLITRSTFGEIALSLLSSGIKHHHHIITKSVNIKDYPCFITVDMSSNSQRKCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFMRSYYRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAIKYQVAQLSKLLENRKVPGEKSNSINLKNIKFGVDQSFPTSSLTDNQILNLLNDIQINMQNEKNHSIEEIEEMKLNFSVLFSALKEKKKNIGKFIEFEEDIENSSNSVNRRFSLPAMTKVLQKNSSKRFSFSKQQIKITDNNEN 673

HSP 2 Score: 582.022 bits (1499), Expect = 0.000e+0
Identity = 301/301 (100.00%), Postives = 301/301 (100.00%), Query Frame = 1 
Query:   19 MSKSRISDFRYYSDQTPDVDYYTNDNLLGSCCSELAIFFICFXXXXXXXXXXXXXXXXXXXVRGIYYLYRLVFMKTKSTEGEIGMSLTFEEARWLSEMSASMIGVLFMDKPVELSELKRVISERIGNLDSSSNKNYQITKRLRQTAQKIDFHWRWVEECEFDLCHHVTLLTPKFTELSDILVEISNKPYDFSHPFWNLYLINSPISSNQGKSCSSTIKINEKKCKLCESNSTIVLKIHPSITDGMCIMKLLSEIFNDMPEPSQLGINNYRKKFCFKAFTALFYSFYIFIKSWSFRQAQYRL 921
            MSKSRISDFRYYSDQTPDVDYYTNDNLLGSCCSELAIFFICFILIGPLISIIGIIWLLSGIVRGIYYLYRLVFMKTKSTEGEIGMSLTFEEARWLSEMSASMIGVLFMDKPVELSELKRVISERIGNLDSSSNKNYQITKRLRQTAQKIDFHWRWVEECEFDLCHHVTLLTPKFTELSDILVEISNKPYDFSHPFWNLYLINSPISSNQGKSCSSTIKINEKKCKLCESNSTIVLKIHPSITDGMCIMKLLSEIFNDMPEPSQLGINNYRKKFCFKAFTALFYSFYIFIKSWSFRQAQYRL
Sbjct:    1 MSKSRISDFRYYSDQTPDVDYYTNDNLLGSCCSELAIFFICFILIGPLISIIGIIWLLSGIVRGIYYLYRLVFMKTKSTEGEIGMSLTFEEARWLSEMSASMIGVLFMDKPVELSELKRVISERIGNLDSSSNKNYQITKRLRQTAQKIDFHWRWVEECEFDLCHHVTLLTPKFTELSDILVEISNKPYDFSHPFWNLYLINSPISSNQGKSCSSTIKINEKKCKLCESNSTIVLKIHPSITDGMCIMKLLSEIFNDMPEPSQLGINNYRKKFCFKAFTALFYSFYIFIKSWSFRQAQYRL 301
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BLAST of SMED30002449 vs. Planmine SMEST
Match: SMESG000020958.1 (SMESG000020958.1)

HSP 1 Score: 169.859 bits (429), Expect = 1.263e-43
Identity = 111/388 (28.61%), Postives = 205/388 (52.84%), Query Frame = 3 
Query:  951 EIPSQLEINSHQFVFCHFSLEEITRLKLITRSTFGEIALSLLSSGIKHHHHIITKSVNIKDYPCFITVDM------------SSNSQRKCG--------IQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSSDYLFFYLLPKFISILFSTSLSKRFMRSYYRKSKVLLSSLNGTWQPILINENKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAIKYQVAQLSKLLENRKVPGEKSNSINL--------KNIKFG----VDQSFP----TSSLTDNQILNLLNDIQINMQNEKNHSIEEIEEMKLNFSVLFSALKEKK----KNIGKFIEFEEDIENSSN--SVNRRFSLPAMTKVLQKNSSKR 1988
            E P +   +   F +C + +  I+R+K +TRSTF E+ L+ ++  ++ +   I+   +  D  C +  D+            SS+   K          +  I N+ +  D  LP +TEGMIP LW++KQ+M+ LK+S+D +    L + +  LF   + +R M +YY K+ + +SSL G    + I     +++++WFP+   +S+S+ CF+Y D FT  I     +  N++L++D+I YQ+  L+KLLE+R++PGE      L         ++K G     D+       + S++ + + + + +IQ  +QN+K+ +I+E++ ++  FS L   L+ +K       G+  +F+E++E  SN  S +RR S   MT ++  ++ +R
Sbjct:  383 ENPEKASEDERIFHYCSYPMSVISRIKGVTRSTFTEVFLAAIAGALRTYQQQISNISHPFDILCLLPTDLTKEKHSTSLGRNSSSVASKTSNMSSITDTLPHIGNNYSLVDFGLPTNTEGMIPRLWEVKQRMEELKNSADSIILSGLSRILWHLFPKIVGQRVMNTYYNKNSLSISSLQGPDCGLSIGGKAVHSIIYWFPILCEVSLSISCFTYADSFTCSISANKLQLPNIRLILDSIVYQMDLLNKLLEHRRIPGENKTQRYLTPSGDEPKSSLKVGKFETTDKELRSQNLSPSVSSDHLYSRIANIQNQLQNDKDLTIDEMDALRSEFSSLMGILRRRKSCGIDTNGEAADFDEELEELSNTPSGSRRQS---MTGLMMLSTRRR 767

HSP 2 Score: 59.6918 bits (143), Expect = 7.681e-9
Identity = 45/184 (24.46%), Postives = 83/184 (45.11%), Query Frame = 1 
Query:  412 SNKNYQITKRLRQTAQKIDFHWRWVEECEFDLCHHV----------TLLTPKFTELSDILVEISNKPYDFSHPFWNLYLINSPISSNQGKSCSSTIKINEKKCKLCESNST----IVLKIHPSITDGMCIMKLLSEIFNDMPEPSQLGINNYRKKFCFKAFTALFYSFYIFIKSWSFRQAQYRL 921
            S+++  I  +  Q   K    W+W  +  FD+ +HV          T   P   +LS ++  I++   D + P W++++I++  S  +  S S        K KL   N++    ++ +IHPS++DG+ ++ +L    +D   P +L    Y       A  ALF    +FI+ W   +  Y +
Sbjct:  151 SSRSDLIYAKFSQKITKSKLGWKWKFDENFDIGNHVFKWKNDVTMSTFSDPD--KLSQLISHINSLHLDMNRPPWDVHIISTTESQLKSNSNSENKPDKTLKEKLNSDNNSKKGLLIFRIHPSLSDGVSLVHILCASLSDEQCPVELKDRFYSSGKFLNAIRALFVGPVVFIQKWFLTRQDYNI 332
The following BLAST results are available for this feature:
BLAST of SMED30002449 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
R7UDU45.431e-2723.46Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A210Q8G41.623e-2628.63Diacylglycerol O-acyltransferase OS=Mizuhopecten y... [more]
A0A0L8I9259.328e-2526.79Uncharacterized protein (Fragment) OS=Octopus bima... [more]
A0A147BBB62.072e-2325.34Putative wax ester synthase-like acyl-coa acyltran... [more]
V4AUH48.363e-2325.31DUF1298 domain-containing protein OS=Lottia gigant... [more]
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BLAST of SMED30002449 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO305381.619e-1021.03Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO402046.900e-721.61Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30002449 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002449 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000051066.10.000e+093.55SMESG000051066.1[more]
SMESG000020958.11.263e-4328.61SMESG000020958.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002449 ID=SMED30002449|Name=SMED30002449|organism=Schmidtea mediterranea sexual|type=transcript|length=2041bp
AAATGATTTTTAGAAATAATGAGCAAATCCCGAATTTCCGATTTTCGATA
TTATTCGGATCAAACACCGGATGTTGACTATTATACTAACGATAATCTAT
TAGGATCATGCTGTTCGGAATTAGCAATATTTTTCATATGCTTCATTCTC
ATTGGTCCACTTATTTCTATTATTGGAATCATTTGGCTGCTATCAGGAAT
CGTTCGTGGAATTTATTATTTATATAGATTGGTATTTATGAAAACAAAGT
CAACTGAAGGAGAAATAGGGATGTCATTAACATTCGAAGAAGCTCGTTGG
CTCTCTGAAATGAGTGCATCAATGATTGGAGTTCTGTTCATGGATAAACC
AGTGGAATTGTCTGAATTGAAAAGAGTGATTTCGGAAAGAATTGGAAATC
TAGATTCAAGTTCGAATAAAAATTATCAAATTACAAAACGACTTCGCCAA
ACTGCTCAAAAGATTGATTTTCATTGGCGATGGGTTGAAGAATGTGAGTT
TGATTTGTGCCATCATGTCACTTTGTTGACTCCCAAATTCACGGAGCTAT
CGGATATTTTAGTTGAAATAAGCAATAAACCTTATGATTTTTCGCATCCA
TTTTGGAATTTATATTTAATTAACTCTCCAATATCGTCAAATCAAGGGAA
GTCTTGCTCATCAACTATAAAAATAAATGAAAAAAAGTGTAAATTATGCG
AATCGAATTCCACAATAGTCCTCAAAATCCATCCTAGCATCACAGATGGA
ATGTGCATAATGAAATTGCTAAGTGAAATATTCAACGATATGCCAGAACC
GTCTCAATTGGGAATAAATAATTATAGAAAAAAGTTTTGTTTCAAAGCGT
TCACTGCACTTTTCTATTCGTTTTATATTTTCATCAAATCATGGTCTTTT
AGACAAGCACAATATCGTTTGTTGATAGTTCCGATTATAAGTCTCCATCG
GAAATTCCTTCACAACTTGAAATAAACAGTCACCAATTTGTCTTTTGCCA
TTTTTCTTTGGAAGAAATAACTCGATTGAAACTCATCACTAGATCCACAT
TTGGGGAAATTGCATTGTCACTTCTGTCAAGTGGAATCAAACACCATCAT
CACATCATTACAAAATCTGTAAACATCAAGGACTATCCATGCTTCATAAC
CGTTGATATGAGTAGTAATTCTCAGAGGAAATGTGGAATTCAAAAAATCA
AAAACGACCTAACTTATTTTGACATCGAATTGCCTTTATCAACAGAAGGA
ATGATTCCAATTCTGTGGAAACTTAAACAAAAAATGAAGACTTTAAAATC
GTCTTCTGATTATTTATTTTTCTATTTATTACCGAAATTTATCTCCATTT
TATTTTCAACAAGTCTTTCTAAAAGATTCATGAGATCCTATTATAGAAAA
TCTAAAGTTTTGCTTTCATCTTTAAATGGAACATGGCAACCGATTTTGAT
CAACGAAAACAAAGCAAACAATCTTCTGTTCTGGTTTCCTTTATTTGAAT
TGATATCAGTTTCCATGTGTTGTTTTTCCTACAATGATCAGTTTACGATT
TGCATCCGAGGACAAAATTCCGAATATGTAAATCTTCAATTGTTAGTTGA
TGCGATTAAGTACCAGGTGGCACAACTTTCTAAACTGCTAGAAAACCGAA
AAGTTCCTGGTGAAAAGTCCAATTCAATTAATTTAAAAAATATTAAATTT
GGTGTCGATCAAAGTTTCCCAACCTCGAGTTTAACAGACAATCAAATTCT
TAATCTTTTAAACGACATTCAAATAAATATGCAAAATGAAAAAAACCATT
CAATCGAAGAAATAGAAGAAATGAAATTGAATTTCTCAGTGCTATTTTCA
GCACTTAAAGAAAAAAAGAAAAATATCGGCAAGTTTATAGAGTTCGAGGA
AGATATTGAAAATTCAAGCAATTCAGTAAATCGCAGGTTTTCTCTACCGG
CAATGACAAAAGTTCTCCAAAAGAATTCGTCCAAGCGATTCAGCTTTTCA
AAGCAACAAATAAAAATTACTGATAACAATGAAAATAAGAG
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protein sequence of SMED30002449-orf-1

>SMED30002449-orf-1 ID=SMED30002449-orf-1|Name=SMED30002449-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=294bp
MSSNSQRKCGIQKIKNDLTYFDIELPLSTEGMIPILWKLKQKMKTLKSSS
DYLFFYLLPKFISILFSTSLSKRFMRSYYRKSKVLLSSLNGTWQPILINE
NKANNLLFWFPLFELISVSMCCFSYNDQFTICIRGQNSEYVNLQLLVDAI
KYQVAQLSKLLENRKVPGEKSNSINLKNIKFGVDQSFPTSSLTDNQILNL
LNDIQINMQNEKNHSIEEIEEMKLNFSVLFSALKEKKKNIGKFIEFEEDI
ENSSNSVNRRFSLPAMTKVLQKNSSKRFSFSKQQIKITDNNENK
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protein sequence of SMED30002449-orf-2

>SMED30002449-orf-2 ID=SMED30002449-orf-2|Name=SMED30002449-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=319bp
MSKSRISDFRYYSDQTPDVDYYTNDNLLGSCCSELAIFFICFILIGPLIS
IIGIIWLLSGIVRGIYYLYRLVFMKTKSTEGEIGMSLTFEEARWLSEMSA
SMIGVLFMDKPVELSELKRVISERIGNLDSSSNKNYQITKRLRQTAQKID
FHWRWVEECEFDLCHHVTLLTPKFTELSDILVEISNKPYDFSHPFWNLYL
INSPISSNQGKSCSSTIKINEKKCKLCESNSTIVLKIHPSITDGMCIMKL
LSEIFNDMPEPSQLGINNYRKKFCFKAFTALFYSFYIFIKSWSFRQAQYR
LLIVPIISLHRKFLHNLK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR004255O-acyltransferase_WSD1_N
IPR009721O-acyltransferase_WSD1_C
Vocabulary: molecular function
TermDefinition
GO:0004144diacylglycerol O-acyltransferase activity
Vocabulary: biological process
TermDefinition
GO:0045017glycerolipid biosynthetic process
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004255O-acyltransferase, WSD1, N-terminalPFAMPF03007WES_acyltransfcoord: 130..202
e-value: 1.9E-6
score: 27.9
NoneNo IPR availablePANTHERPTHR31650:SF1O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 109..263
NoneNo IPR availablePANTHERPTHR31650FAMILY NOT NAMEDcoord: 109..263
NoneNo IPR availableTMHMMTMhelixcoord: 35..57