SMED30002363

Overview
NameSMED30002363
Smed IDSMED30002363
Length (bp)3153
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002363 (SMED30002363) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002363 (SMED30002363) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002363 (SMED30002363) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002363 (SMED30002363) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002363

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30002363SMESG000050280.1 dd_Smed_v4_7330_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002363SMESG000050280.1 dd_Smed_v4_7330_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30002363 vs. TrEMBL
Match: A0A024U7W1 (Uncharacterized protein OS=Aphanomyces invadans OX=157072 GN=H310_06012 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 4.430e-9
Identity = 48/170 (28.24%), Postives = 77/170 (45.29%), Query Frame = 2 
Query: 2150 KTLSKELHDFVGLHYTEQWCQMHVQGHIADAVGDSITAKYLITNDILNDAQYSFLIHARNNLLKLNFTPYRLKYNIGAKCRLCHQDE-ETQAHVFNHCPAKPNARRIKHENVLISVVTFLEKIWFEIDL--EKSLKYISIPTKLKPDMVIRSKKNKDIPVFDLKIPYDSG 2650
            KT+ ++L   +   +   WC++  QG    A  + + +++L     L D +Y F I AR NL+       R   N  + CR C + E ET  HV  HCP    + R +H+  L  +   + K      L  E+ L   +    LKPD+++    NK   + DL + +D G
Sbjct:   38 KTVLRQLKTHMRTMHYNAWCELQDQGRTVGAH-EGLGSRFLSRGGGLLDCEYRFAIAARMNLIATRAVLKRTHGNSNSSCRHCGRTETETLPHVLQHCPHNEASIRARHDLSLRCIAAAIAKANPSCRLLVEQPLPEFT-DALLKPDIILVDDANKTAAICDLAVSFDDG 205
[Close]
BLAST of SMED30002363 vs. TrEMBL
Match: A0A418AIC4 (Uncharacterized protein OS=Aphanomyces invadans OX=157072 GN=DYB32_009474 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 4.595e-9
Identity = 48/170 (28.24%), Postives = 77/170 (45.29%), Query Frame = 2 
Query: 2150 KTLSKELHDFVGLHYTEQWCQMHVQGHIADAVGDSITAKYLITNDILNDAQYSFLIHARNNLLKLNFTPYRLKYNIGAKCRLCHQDE-ETQAHVFNHCPAKPNARRIKHENVLISVVTFLEKIWFEIDL--EKSLKYISIPTKLKPDMVIRSKKNKDIPVFDLKIPYDSG 2650
            KT+ ++L   +   +   WC++  QG    A  + + +++L     L D +Y F I AR NL+       R   N  + CR C + E ET  HV  HCP    + R +H+  L  +   + K      L  E+ L   +    LKPD+++    NK   + DL + +D G
Sbjct:   38 KTVLRQLKTHMRTMHYNAWCELQDQGRTVGAH-EGLGSRFLSRGGGLLDCEYRFAIAARMNLIATRAVLKRTHGNSNSSCRHCGRTETETLPHVLQHCPHNEASIRARHDLSLRCIAAAIAKANPSCRLLVEQPLPEFT-DALLKPDIILVDDANKTAAICDLAVSFDDG 205
[Close]
BLAST of SMED30002363 vs. TrEMBL
Match: K7JWY8 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.801e-7
Identity = 41/175 (23.43%), Postives = 80/175 (45.71%), Query Frame = 2 
Query: 2126 SYINIQNLKTLSKELHDFVGLHYTEQWCQMHVQGHIADAVGDSITAK-YLITNDILNDAQYSFLIHARNNLLKLNFTPYRLKYNIGAKCRLCHQDEETQAHVFNHCPAKPNARRIKHENVLISVVTFLEKIWFEIDLEKSLKYISIP-TKLKPDMVIRSKKNKDIPVFDLKIPYD 2644
            + ++ Q    ++  L   +  HY E       QG + +    S  +  +L +        + F+  AR ++L LN    R +  +  +CR C  D ET  HV +HC     AR+ +H N+   +V    +    I + +++  +  P   L+PD+V+R   N+ + + D+ +P++
Sbjct:   93 TRVSPQTKHLVTTSLRRCLNRHYAESLLAKKDQGKVFEVTRRSGQSNHFLRSGSFTRFCDWRFIHRARLDVLPLN-AAKRWQRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFE 266
[Close]
BLAST of SMED30002363 vs. TrEMBL
Match: K7JS44 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.833e-7
Identity = 41/175 (23.43%), Postives = 80/175 (45.71%), Query Frame = 2 
Query: 2126 SYINIQNLKTLSKELHDFVGLHYTEQWCQMHVQGHIADAVGDSITAK-YLITNDILNDAQYSFLIHARNNLLKLNFTPYRLKYNIGAKCRLCHQDEETQAHVFNHCPAKPNARRIKHENVLISVVTFLEKIWFEIDLEKSLKYISIP-TKLKPDMVIRSKKNKDIPVFDLKIPYD 2644
            + ++ Q    ++  L   +  HY E       QG + +    S  +  +L +        + F+  AR ++L LN    R +  +  +CR C  D ET  HV +HC     AR+ +H N+   +V    +    I + +++  +  P   L+PD+V+R   N+ + + D+ +P++
Sbjct:   93 TRVSPQTKHLVTTSLRRCLNRHYAESLLAKKDQGKVFEVTRRSGQSNHFLRSGSFTRFCDWRFIHRARLDVLPLN-AAKRWQRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFE 266
[Close]
BLAST of SMED30002363 vs. TrEMBL
Match: K7JS43 (C2H2-type domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 1.833e-7
Identity = 41/175 (23.43%), Postives = 80/175 (45.71%), Query Frame = 2 
Query: 2126 SYINIQNLKTLSKELHDFVGLHYTEQWCQMHVQGHIADAVGDSITAK-YLITNDILNDAQYSFLIHARNNLLKLNFTPYRLKYNIGAKCRLCHQDEETQAHVFNHCPAKPNARRIKHENVLISVVTFLEKIWFEIDLEKSLKYISIP-TKLKPDMVIRSKKNKDIPVFDLKIPYD 2644
            + ++ Q    ++  L   +  HY E       QG + +    S  +  +L +        + F+  AR ++L LN    R +  +  +CR C  D ET  HV +HC     AR+ +H N+   +V    +    I + +++  +  P   L+PD+V+R   N+ + + D+ +P++
Sbjct:   93 TRVSPQTKHLVTTSLRRCLNRHYAESLLAKKDQGKVFEVTRRSGQSNHFLRSGSFTRFCDWRFIHRARLDVLPLN-AAKRWQRGMDKRCRRCGSDLETLPHVLSHCGPHSAARQKRHNNIQDRLVKAASRCPGTISVNQTVVGVRGPDAALRPDIVVRDDVNRRVTIVDVAVPFE 266
[Close]
BLAST of SMED30002363 vs. Planmine SMEST
Match: SMESG000050280.1 (SMESG000050280.1)

HSP 1 Score: 928.702 bits (2399), Expect = 0.000e+0
Identity = 471/472 (99.79%), Postives = 471/472 (99.79%), Query Frame = 2 
Query:  137 MKSMISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTXXXXXXXXXXXXXXCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ 1552
            MK MISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTIGVSVVFIGIFILVCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ
Sbjct:    1 MKFMISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTIGVSVVFIGIFILVCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ 472
[Close]
BLAST of SMED30002363 vs. Planmine SMEST
Match: SMESG000050280.1 (SMESG000050280.1)

HSP 1 Score: 925.62 bits (2391), Expect = 0.000e+0
Identity = 469/469 (100.00%), Postives = 469/469 (100.00%), Query Frame = 2 
Query:  146 MISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTXXXXXXXXXXXXXXCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ 1552
            MISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTIGVSVVFIGIFILVCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ
Sbjct:    1 MISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYAVDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAFVVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFWIGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCPNVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYEIKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAISHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFNERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNIIQLTIGVSVVFIGIFILVCLFRGNLKKLNDHVTINEYENGDHILVRCSSIKNISDPIRKNDTTEESIYETISQ 469
[Close]
BLAST of SMED30002363 vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 343.584 bits (880), Expect = 2.331e-124
Identity = 181/259 (69.88%), Postives = 213/259 (82.24%), Query Frame = 2 
Query: 1883 NDIPFGFILRIHLYSEARW*KNFEAGLKGMNGEVSKQFPNTSXXXXXXXXXXXXXXXXCICFKLNFVDDTFSLEMSYKKRISYINIQNLKTLSKELHDFVGLHYTEQWCQMHVQGHIADAVGDSITAKYLITNDILNDAQYSFLIHARNNLLKLNFTPYRLKYNIGAKCRLCHQDEETQAHVFNHCPAKPNARRIKHENVLISVVTFLEKIWFEIDLEKSLKYISIPTKLKPDMVIRSKKNKDIPVFDLKIPYDSGENF 2659
            ++I F  +L   L +++R   NFEAGL  MN E +K F  +SFF KFQK+A +LKRKFCI   L F++D F+LE++YKKRISYIN QNL TLSKELH+F+GLHY EQWC+M VQGHIA ++GDSITAKYLI +D LNDAQY FL+ ARNNLLKLN+  YRLKYNI  KCRLCH DEETQAHVFNHC AKPNARR+KHENVL+ +V+FLEKI FEID+EKS KY+SIPTKLKPDMVIRSK+N+DI V DLK+PYDS E F
Sbjct: 2190 SNISFRKMLIAEL-AQSRGNGNFEAGLSWMNCEFNKIFLKSSFFVKFQKSAQSLKRKFCIYVNLKFIEDNFTLEITYKKRISYINNQNLNTLSKELHNFIGLHYAEQWCKMRVQGHIAASIGDSITAKYLIASDTLNDAQYYFLVRARNNLLKLNYGAYRLKYNINTKCRLCHLDEETQAHVFNHCQAKPNARRVKHENVLLGIVSFLEKIGFEIDVEKSPKYVSIPTKLKPDMVIRSKRNRDIHVLDLKVPYDSIEGF 2447

HSP 2 Score: 123.635 bits (309), Expect = 2.331e-124
Identity = 61/84 (72.62%), Postives = 69/84 (82.14%), Query Frame = 1 
Query: 2662 KARERNYGKYKDLDLEIGHAFRKKETISALVIGCLGTRDKKKNQALSKIILTKAEIQSLARIACPNAVIACYHIYREHISYTKS 2913
            KARE NY KYKDL ++IG AF +  TISA+VIGCLGT DKK N  LSKI LTK EI +LA+IAC +AVIACYHIYREHIS+TKS
Sbjct: 2449 KAREDNYVKYKDLAMQIGKAFNQTATISAVVIGCLGTWDKKNNATLSKIGLTKTEIIALAKIACSDAVIACYHIYREHISFTKS 2532
[Close]
BLAST of SMED30002363 vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 343.584 bits (880), Expect = 2.533e-124
Identity = 181/259 (69.88%), Postives = 213/259 (82.24%), Query Frame = 2 
Query: 1883 NDIPFGFILRIHLYSEARW*KNFEAGLKGMNGEVSKQFPNTSXXXXXXXXXXXXXXXXCICFKLNFVDDTFSLEMSYKKRISYINIQNLKTLSKELHDFVGLHYTEQWCQMHVQGHIADAVGDSITAKYLITNDILNDAQYSFLIHARNNLLKLNFTPYRLKYNIGAKCRLCHQDEETQAHVFNHCPAKPNARRIKHENVLISVVTFLEKIWFEIDLEKSLKYISIPTKLKPDMVIRSKKNKDIPVFDLKIPYDSGENF 2659
            ++I F  +L   L +++R   NFEAGL  MN E +K F  +SFF KFQK+A +LKRKFCI   L F++D F+LE++YKKRISYIN QNL TLSKELH+F+GLHY EQWC+M VQGHIA ++GDSITAKYLI +D LNDAQY FL+ ARNNLLKLN+  YRLKYNI  KCRLCH DEETQAHVFNHC AKPNARR+KHENVL+ +V+FLEKI FEID+EKS KY+SIPTKLKPDMVIRSK+N+DI V DLK+PYDS E F
Sbjct: 2169 SNISFRKMLIAEL-AQSRGNGNFEAGLSWMNCEFNKIFLKSSFFVKFQKSAQSLKRKFCIYVNLKFIEDNFTLEITYKKRISYINNQNLNTLSKELHNFIGLHYAEQWCKMRVQGHIAASIGDSITAKYLIASDTLNDAQYYFLVRARNNLLKLNYGAYRLKYNINTKCRLCHLDEETQAHVFNHCQAKPNARRVKHENVLLGIVSFLEKIGFEIDVEKSPKYVSIPTKLKPDMVIRSKRNRDIHVLDLKVPYDSIEGF 2426

HSP 2 Score: 123.635 bits (309), Expect = 2.533e-124
Identity = 61/84 (72.62%), Postives = 69/84 (82.14%), Query Frame = 1 
Query: 2662 KARERNYGKYKDLDLEIGHAFRKKETISALVIGCLGTRDKKKNQALSKIILTKAEIQSLARIACPNAVIACYHIYREHISYTKS 2913
            KARE NY KYKDL ++IG AF +  TISA+VIGCLGT DKK N  LSKI LTK EI +LA+IAC +AVIACYHIYREHIS+TKS
Sbjct: 2428 KAREDNYVKYKDLAMQIGKAFNQTATISAVVIGCLGTWDKKNNATLSKIGLTKTEIIALAKIACSDAVIACYHIYREHISFTKS 2511
[Close]
BLAST of SMED30002363 vs. Planmine SMEST
Match: SMESG000002829.1 (SMESG000002829.1)

HSP 1 Score: 343.584 bits (880), Expect = 2.555e-124
Identity = 181/259 (69.88%), Postives = 213/259 (82.24%), Query Frame = 2 
Query: 1883 NDIPFGFILRIHLYSEARW*KNFEAGLKGMNGEVSKQFPNTSXXXXXXXXXXXXXXXXCICFKLNFVDDTFSLEMSYKKRISYINIQNLKTLSKELHDFVGLHYTEQWCQMHVQGHIADAVGDSITAKYLITNDILNDAQYSFLIHARNNLLKLNFTPYRLKYNIGAKCRLCHQDEETQAHVFNHCPAKPNARRIKHENVLISVVTFLEKIWFEIDLEKSLKYISIPTKLKPDMVIRSKKNKDIPVFDLKIPYDSGENF 2659
            ++I F  +L   L +++R   NFEAGL  MN E +K F  +SFF KFQK+A +LKRKFCI   L F++D F+LE++YKKRISYIN QNL TLSKELH+F+GLHY EQWC+M VQGHIA ++GDSITAKYLI +D LNDAQY FL+ ARNNLLKLN+  YRLKYNI  KCRLCH DEETQAHVFNHC AKPNARR+KHENVL+ +V+FLEKI FEID+EKS KY+SIPTKLKPDMVIRSK+N+DI V DLK+PYDS E F
Sbjct: 2198 SNISFRKMLIAEL-AQSRGNGNFEAGLSWMNCEFNKIFLKSSFFVKFQKSAQSLKRKFCIYVNLKFIEDNFTLEITYKKRISYINNQNLNTLSKELHNFIGLHYAEQWCKMRVQGHIAASIGDSITAKYLIASDTLNDAQYYFLVRARNNLLKLNYGAYRLKYNINTKCRLCHLDEETQAHVFNHCQAKPNARRVKHENVLLGIVSFLEKIGFEIDVEKSPKYVSIPTKLKPDMVIRSKRNRDIHVLDLKVPYDSIEGF 2455

HSP 2 Score: 123.635 bits (309), Expect = 2.555e-124
Identity = 61/84 (72.62%), Postives = 69/84 (82.14%), Query Frame = 1 
Query: 2662 KARERNYGKYKDLDLEIGHAFRKKETISALVIGCLGTRDKKKNQALSKIILTKAEIQSLARIACPNAVIACYHIYREHISYTKS 2913
            KARE NY KYKDL ++IG AF +  TISA+VIGCLGT DKK N  LSKI LTK EI +LA+IAC +AVIACYHIYREHIS+TKS
Sbjct: 2457 KAREDNYVKYKDLAMQIGKAFNQTATISAVVIGCLGTWDKKNNATLSKIGLTKTEIIALAKIACSDAVIACYHIYREHISFTKS 2540
The following BLAST results are available for this feature:
BLAST of SMED30002363 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A024U7W14.430e-928.24Uncharacterized protein OS=Aphanomyces invadans OX... [more]
A0A418AIC44.595e-928.24Uncharacterized protein OS=Aphanomyces invadans OX... [more]
K7JWY81.801e-723.43C2H2-type domain-containing protein OS=Nasonia vit... [more]
K7JS441.833e-723.43C2H2-type domain-containing protein OS=Nasonia vit... [more]
K7JS431.833e-723.43C2H2-type domain-containing protein OS=Nasonia vit... [more]
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BLAST of SMED30002363 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002363 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000050280.10.000e+099.79SMESG000050280.1[more]
SMESG000050280.10.000e+0100.00SMESG000050280.1[more]
SMESG000002829.12.331e-12469.88SMESG000002829.1[more]
SMESG000002829.12.533e-12469.88SMESG000002829.1[more]
SMESG000002829.12.555e-12469.88SMESG000002829.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002363 ID=SMED30002363|Name=SMED30002363|organism=Schmidtea mediterranea sexual|type=transcript|length=3153bp
TTTCAAACAAATCGTCAGATCTATGTTTAACAATAATTTAAACAAATCAT
AAAATGACATTAATATATCTTATTTGTTCGATGTATTAATTTAATTATAA
ATAAAAGTTTATTTAAACAAAAGTTAATATTTTATTATGAAGTCTATGAT
CTCATTTTTGTTAACAAATTTTCTCATTTCTTTTATCTCAGCAAACTATA
CTTACAGTGAAAGTCAGGGAGATCTTCAAAAGGAATTCAACTTTTCGCTG
TTCATTGAAACATTCAGTGGAATTACTCTCTATGCCGTCGATTCCAATAA
TAATAAATTTCTGATAGAAATGGAACTTGAAGAAAATTTGCAAATCGCGA
TAATAAAATACAACAAGTTGTTCAATCGAGTAAAATTAGAAAATTTATGC
GGGTGCCGATTTTGCTCCCATGCCAGCATGGCATTCGTTGTCCGGTTACG
TCGGTTGGAGGTGAATGCAAAATGTAAAGGAGTTGACAAATTGATAAGAT
TGGAAAATGTCAATGGTGACGGTCAATTTAACGGATACCCATTACCGAGG
AAACGTTCTAATAATACAAACAAAATATATTTCTGGATCGGAAATATTGG
TTATTACTTTGGATTGGGTAAAATTTTATATGTACCATTAAATAACCAAT
TAACGGGTTGCTTGGACAAATTTTATTTCAATAACCGATTTCTAACCTTT
GAACAGAAAGGTAAAATGATGTTGAACTCGTGCCCTAATGTGTTCAATGA
ATCGGTTTGCAATGAAACCTCTTGTATGGCTGATCAGATATCATACGGCA
TAAATGACTGCGCTCTTGCGTCCGTGAATCCAAGATATTGTAGAAAAGCT
TTGCCATTATATTCGAAATCAAAAGATTTATACGAAATCAAGATACGAGC
GAAATTCCCAATTACGATCCGATTAGAGTGGAAATCATTCTACTTGAAGA
ATTTCTGTTTAATTGACATCAAAATGGGAAAAAACAAGTTCATTACAATG
TATTTAAACAAAATGAATCAAATATCTTTTGCAATAAGTCACAACCGCTC
TATTGTTGTTGAGAGAAATTTAACGAAAATGAAAGAGCTGGACAATAATT
TGTTAATTATCATTTTTAACGTTACTCAATTCATGATTAGAGCGGCAAGT
GCGAATGAAATATACACGTTTATTGAACCGTTTAATGAACGACCAATGGA
AATGCGAATCGGAAGTGGTGGGAATATAAATATCTGTCAAACCTTTGAAG
GGGCCATTGATATTTTTTACGATCCAACTCAAATCAGCTTACGAACAGGT
CAAAACCATTTTCAACAATCAAAGTTTAATATAATTCAACTTACCATTGG
TGTTTCGGTTGTTTTTATTGGAATCTTTATTTTAGTATGTCTTTTCAGAG
GCAATCTTAAAAAATTAAATGACCATGTAACCATAAATGAGTATGAAAAT
GGCGATCACATTCTTGTAAGATGCTCTTCCATCAAAAATATATCAGACCC
GATTCGGAAAAATGACACAACGGAAGAATCTATTTACGAAACTATATCCC
AATAAATTCTTTGTCATTGGCTTAATTATTTAAAATTTTGAATGGTCAGC
TATGCGGTTAGCTTTACTTGAAATGGAGTTGATTAATGTTTTAACCTATT
CTTCAATATTTCTCCGATTAAATTATATACTATGTATATAATCTGCTATT
AACCAATGAGACATTTTTATGGTGCAAAGAAAATACACAAACGCTGAATT
AACTAATAATTGGCTGCCTATATTTTTCCTACTGAAATATATGAATTGAA
CCGGATGTTATTTGTAGTAAGTTAAAAAATTCTAAAACAAAACATGAATC
AGATTTGATATATTCCACAAACATAGAAATTAAATGATATTCCATTCGGT
TTTATTTTGAGGATTCATTTGTATTCTGAAGCACGATGGTAGAAAAACTT
TGAAGCAGGACTTAAAGGGATGAATGGCGAGGTATCCAAGCAATTTCCTA
ATACTTCTTTCTTCAAAAAATTTCAGAAAGCAGCACTTGCTCTTAAAAGA
AAATTCTGTATATGCTTTAAACTTAACTTTGTAGATGACACTTTTTCACT
TGAAATGTCCTACAAAAAACGCATCTCTTATATAAATATACAGAATTTAA
AAACTCTTTCTAAAGAACTTCACGACTTCGTGGGTCTTCATTATACTGAG
CAATGGTGTCAAATGCACGTGCAAGGACACATTGCAGATGCGGTTGGGGA
TAGCATTACGGCTAAATACCTCATAACTAATGACATTCTTAACGATGCAC
AATACAGCTTCTTGATACATGCAAGGAACAATCTTCTTAAACTTAACTTT
ACTCCTTATCGTCTTAAATATAATATCGGCGCAAAATGCAGATTGTGTCA
CCAAGACGAGGAGACTCAGGCCCATGTGTTCAATCACTGTCCTGCCAAGC
CTAACGCTAGAAGAATTAAACATGAAAATGTATTAATAAGCGTAGTTACC
TTCTTAGAGAAAATTTGGTTTGAGATTGATTTGGAAAAATCACTCAAATA
TATCTCAATACCAACAAAGTTAAAACCTGACATGGTAATCAGGTCTAAAA
AGAACAAAGATATACCTGTTTTTGACTTGAAAATACCATATGACTCAGGG
GAAAACTTTTAAAAGCGCGAGAACGTAACTACGGAAAATACAAAGATCTA
GATTTAGAAATTGGACATGCCTTCAGGAAGAAAGAAACAATATCTGCTTT
GGTGATTGGATGCCTGGGAACACGGGATAAAAAGAAAAACCAAGCTCTCT
CCAAGATTATATTAACTAAGGCTGAAATCCAATCTCTTGCACGGATAGCA
TGCCCAAATGCAGTAATCGCATGCTATCACATCTACCGTGAGCACATCTC
TTATACAAAAAGTGCCATGGCCCTTCCTTTCAGCCTGACCTGAGTTTAGA
ATGCTGGTAATTACATCGGCGTTGCAGACTTGTGTATGTAATCTAAAAAA
CAGTAGAAATAGATGCTGAGCCCAGGTCGCATAGTTTAGCCGAAAGGCCG
CTTTAGGTAAATTTAAAAAAACAATTATGAAAAAAAAGAAGGGGACCTTA
ACGAGTGGGACAATAAACCTTCTACGGTAGTCCATGAACCTATGGGACGT
TAA
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protein sequence of SMED30002363-orf-1

>SMED30002363-orf-1 ID=SMED30002363-orf-1|Name=SMED30002363-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=473bp
MKSMISFLLTNFLISFISANYTYSESQGDLQKEFNFSLFIETFSGITLYA
VDSNNNKFLIEMELEENLQIAIIKYNKLFNRVKLENLCGCRFCSHASMAF
VVRLRRLEVNAKCKGVDKLIRLENVNGDGQFNGYPLPRKRSNNTNKIYFW
IGNIGYYFGLGKILYVPLNNQLTGCLDKFYFNNRFLTFEQKGKMMLNSCP
NVFNESVCNETSCMADQISYGINDCALASVNPRYCRKALPLYSKSKDLYE
IKIRAKFPITIRLEWKSFYLKNFCLIDIKMGKNKFITMYLNKMNQISFAI
SHNRSIVVERNLTKMKELDNNLLIIIFNVTQFMIRAASANEIYTFIEPFN
ERPMEMRIGSGGNINICQTFEGAIDIFYDPTQISLRTGQNHFQQSKFNII
QLTIGVSVVFIGIFILVCLFRGNLKKLNDHVTINEYENGDHILVRCSSIK
NISDPIRKNDTTEESIYETISQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.866
NoneNo IPR availableTMHMMTMhelixcoord: 398..420