Mediator of RNA polymerase II transcription subunit 22

Overview
NameMediator of RNA polymerase II transcription subunit 22
Smed IDSMED30002318
Length (bp)733
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Mediator of RNA polymerase II transcription subunit 22 (SMED30002318) t-SNE clustered cells

Violin plots show distribution of expression levels for Mediator of RNA polymerase II transcription subunit 22 (SMED30002318) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Mediator of RNA polymerase II transcription subunit 22 (SMED30002318) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Mediator of RNA polymerase II transcription subunit 22 (SMED30002318) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002318

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30002318SMESG000029018.1 SmedASXL_019748SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30002318SMESG000029018.1 dd_Smed_v4_8119_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Human
Match: MED22 (mediator complex subunit 22 [Source:HGNC Symbol;Acc:HGNC:11477])

HSP 1 Score: 70.4774 bits (171), Expect = 7.957e-15
Identity = 39/129 (30.23%), Postives = 79/129 (61.24%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKE 465
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E  ++  ++    ++  +R +  L+D +++DL  +++E
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------DETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Human
Match: MED22 (mediator complex subunit 22 [Source:HGNC Symbol;Acc:HGNC:11477])

HSP 1 Score: 70.4774 bits (171), Expect = 7.957e-15
Identity = 39/129 (30.23%), Postives = 79/129 (61.24%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKE 465
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E  ++  ++    ++  +R +  L+D +++DL  +++E
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------DETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Human
Match: MED22 (mediator complex subunit 22 [Source:HGNC Symbol;Acc:HGNC:11477])

HSP 1 Score: 70.4774 bits (171), Expect = 9.148e-15
Identity = 39/129 (30.23%), Postives = 79/129 (61.24%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKE 465
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E  ++  ++    ++  +R +  L+D +++DL  +++E
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------DETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Human
Match: MED22 (mediator complex subunit 22 [Source:HGNC Symbol;Acc:HGNC:11477])

HSP 1 Score: 70.4774 bits (171), Expect = 9.148e-15
Identity = 39/129 (30.23%), Postives = 79/129 (61.24%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKE 465
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E  ++  ++    ++  +R +  L+D +++DL  +++E
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------DETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Human
Match: MED22 (mediator complex subunit 22 [Source:HGNC Symbol;Acc:HGNC:11477])

HSP 1 Score: 70.4774 bits (171), Expect = 1.071e-14
Identity = 39/129 (30.23%), Postives = 79/129 (61.24%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKE 465
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E  ++  ++    ++  +R +  L+D +++DL  +++E
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------DETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Celegans
Match: mdt-22 (Mediator of RNA polymerase II transcription subunit 22 [Source:UniProtKB/Swiss-Prot;Acc:Q23679])

HSP 1 Score: 46.9802 bits (110), Expect = 1.818e-6
Identity = 30/120 (25.00%), Postives = 64/120 (53.33%), Query Frame = 1 
Query:  100 LKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVK---KENIMKRQRIERCICDLQDNVAMDLE 450
             K+R   NI++L +NF +I+  +K++  D +  +++    T+      EM VR+  MV  ++ LL++ +++K+ L L DF ++    ++ +   +E + ++     C+     N+  DLE
Sbjct:   27 FKRRLRDNIKSLNDNFFHIIQAAKVNPDDNA-YKNQTGKMTEFYTTKNEMAVRAQLMVRASDELLKLTADLKEFLILHDFHFLTHNIKQAEAQCEETLRQQSHQHNCLDSEVSNILFDLE 145
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Fly
Match: MED22 (gene:FBgn0040339 transcript:FBtr0070165)

HSP 1 Score: 66.6254 bits (161), Expect = 1.391e-13
Identity = 43/132 (32.58%), Postives = 73/132 (55.30%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERC---ICDLQDNVAMDLEAIDKE 465
            K++ L +   R   ++R++LENF+ IL  ++       +   +I+  TQ E D  EM VR+ANMV   ESL+++V+++KQ L L DF  + E    +   + + R     C   +  L+D +AMDL  +++E
Sbjct:   14 KEALLKSYNARLKDDVRSMLENFEEILKLAR------RESHSQISKTTQCEQDALEMQVRAANMVRAGESLMKLVADLKQYLILNDFHSVNEA---ITNNSQLFRNTQSECDKKLMKLRDEMAMDLYDLEEE 136
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Zebrafish
Match: med22 (mediator complex subunit 22 [Source:NCBI gene;Acc:415234])

HSP 1 Score: 72.4034 bits (176), Expect = 1.137e-15
Identity = 46/132 (34.85%), Postives = 76/132 (57.58%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERC---ICDLQDNVAMDLEAIDKE 465
            K++ L N  +R   +IR++L+NF  I+  +K++ +    R       TQAE D +EM+VR+AN+V   ESL+++VS++KQ L L DF  + E    +   N   R   E C   +  L+D +A+DL  +++E
Sbjct:   11 KETLLQNYNKRLKDDIRSILDNFTEIIKTAKVEDETQVSR------ATQAEQDHFEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEA---ISLRNQQLRTLQEECDKKLISLRDEIAIDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Zebrafish
Match: med22 (mediator complex subunit 22 [Source:NCBI gene;Acc:415234])

HSP 1 Score: 72.4034 bits (176), Expect = 1.137e-15
Identity = 46/132 (34.85%), Postives = 76/132 (57.58%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERC---ICDLQDNVAMDLEAIDKE 465
            K++ L N  +R   +IR++L+NF  I+  +K++ +    R       TQAE D +EM+VR+AN+V   ESL+++VS++KQ L L DF  + E    +   N   R   E C   +  L+D +A+DL  +++E
Sbjct:   11 KETLLQNYNKRLKDDIRSILDNFTEIIKTAKVEDETQVSR------ATQAEQDHFEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEA---ISLRNQQLRTLQEECDKKLISLRDEIAIDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Zebrafish
Match: med22 (mediator complex subunit 22 [Source:NCBI gene;Acc:415234])

HSP 1 Score: 72.4034 bits (176), Expect = 4.493e-15
Identity = 45/132 (34.09%), Postives = 78/132 (59.09%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERC---ICDLQDNVAMDLEAIDKE 465
            K++ L N  +R   +IR++L+NF  I+  +K++ +       +++  TQAE D +EM+VR+AN+V   ESL+++VS++KQ L L DF  +    E +   N   R   E C   +  L+D +A+DL  +++E
Sbjct:   11 KETLLQNYNKRLKDDIRSILDNFTEIIKTAKVEDET------QVSRATQAEQDHFEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSV---NEAISLRNQQLRTLQEECDKKLISLRDEIAIDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Xenopus
Match: shc1 (SHC (Src homology 2 domain containing) transforming protein 1 [Source:Xenbase;Acc:XB-GENE-494862])

HSP 1 Score: 70.4774 bits (171), Expect = 2.863e-14
Identity = 39/122 (31.97%), Postives = 74/122 (60.66%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMD 444
            K++ L +  +R   +I+++++NF  I+  +KID         +++  TQ E D YEM+VRSAN+V   ESL+++VS++KQ L L DF  + E+  +  ++    +   ++ +  L+D++A+D
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKID------EEHQVSRSTQGEQDNYEMHVRSANIVRAGESLMKLVSDLKQFLILNDFPSVNESINQRNQQLRALQDECDKKLIALRDDIAID 126
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Mouse
Match: Med22 (mediator complex subunit 22 [Source:MGI Symbol;Acc:MGI:98446])

HSP 1 Score: 70.8626 bits (172), Expect = 4.714e-15
Identity = 40/129 (31.01%), Postives = 79/129 (61.24%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKE 465
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E  ++  ++    ++  +R +  L+D V++DL  +++E
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------DETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRDEVSIDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Mouse
Match: Med22 (mediator complex subunit 22 [Source:MGI Symbol;Acc:MGI:98446])

HSP 1 Score: 68.9366 bits (167), Expect = 7.881e-14
Identity = 38/122 (31.15%), Postives = 74/122 (60.66%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMD 444
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E  ++  ++    ++  +R +  L+D V++D
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------DETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRDEVSID 126
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. UniProt/SwissProt
Match: sp|A5WUL3|MED22_DANRE (Mediator of RNA polymerase II transcription subunit 22 OS=Danio rerio OX=7955 GN=med22 PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 3.461e-14
Identity = 45/132 (34.09%), Postives = 78/132 (59.09%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERC---ICDLQDNVAMDLEAIDKE 465
            K++ L N  +R   +IR++L+NF  I+  +K++ +       +++  TQAE D +EM+VR+AN+V   ESL+++VS++KQ L L DF  +    E +   N   R   E C   +  L+D +A+DL  +++E
Sbjct:   11 KETLLQNYNKRLKDDIRSILDNFTEIIKTAKVEDET------QVSRATQAEQDHFEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSV---NEAISLRNQQLRTLQEECDKKLISLRDEIAIDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. UniProt/SwissProt
Match: sp|Q6GQ95|MED22_XENLA (Mediator of RNA polymerase II transcription subunit 22 OS=Xenopus laevis OX=8355 GN=med22 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 6.797e-14
Identity = 39/122 (31.97%), Postives = 75/122 (61.48%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMD 444
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VRSAN+V   ESL+++VS++KQ L L DF  + E+  +  ++    R+  ++ +  L+D++A+D
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------EEHQVSRSTQGEQDNYEMHVRSANIVRAGESLMKLVSDLKQFLILNDFPSVNESINQRNQQLRTLREECDKKLIALRDDIAID 126
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. UniProt/SwissProt
Match: sp|A0JPN6|MED22_RAT (Mediator of RNA polymerase II transcription subunit 22 OS=Rattus norvegicus OX=10116 GN=Med22 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 5.241e-13
Identity = 38/122 (31.15%), Postives = 74/122 (60.66%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMD 444
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E  ++  ++    ++  +R +  L+D V++D
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------DETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRDEVSID 126
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. UniProt/SwissProt
Match: sp|Q62276|MED22_MOUSE (Mediator of RNA polymerase II transcription subunit 22 OS=Mus musculus OX=10090 GN=Med22 PE=1 SV=2)

HSP 1 Score: 68.9366 bits (167), Expect = 5.513e-13
Identity = 38/122 (31.15%), Postives = 74/122 (60.66%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMD 444
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E  ++  ++    ++  +R +  L+D V++D
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------DETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRALQEECDRKLITLRDEVSID 126
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. UniProt/SwissProt
Match: sp|Q15528|MED22_HUMAN (Mediator of RNA polymerase II transcription subunit 22 OS=Homo sapiens OX=9606 GN=MED22 PE=1 SV=2)

HSP 1 Score: 68.5514 bits (166), Expect = 8.015e-13
Identity = 37/122 (30.33%), Postives = 74/122 (60.66%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMD 444
            K++ L +  +R   +I+++++NF  I+  +KI+         +++  TQ E D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E  ++  ++    ++  +R +  L+D +++D
Sbjct:   11 KETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIE------DETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISID 126
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. TrEMBL
Match: A0A4E0RSD6 (Mediator of RNA polymerase II transcription subunit OS=Fasciola hepatica OX=6192 GN=D915_005352 PE=4 SV=1)

HSP 1 Score: 112.464 bits (280), Expect = 7.568e-27
Identity = 59/130 (45.38%), Postives = 86/130 (66.15%), Query Frame = 1 
Query:   76 KKDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKE 465
            K+DS++ +LK R   N+  + EN++ IL+R+K+D  D S     +N Y QAE D YEM+VR+AN+V+  E+L R+VSEIKQLL LGDF W+  TT    +E   +R  ++R    L+D +A DL AI++E
Sbjct:   18 KRDSHVQSLKTRLRKNMNAITENYELILSRAKVDTNDNSA---ILNAYAQAEQDTYEMSVRAANIVHACENLTRLVSEIKQLLILGDFCWLATTTATASEELRKRRADLDRVSTRLRDKLAADLYAIEEE 144
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. TrEMBL
Match: A0A504YZ59 (Mediator of RNA polymerase II transcription subunit OS=Fasciola gigantica OX=46835 GN=FGIG_07071 PE=4 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 4.576e-26
Identity = 58/130 (44.62%), Postives = 85/130 (65.38%), Query Frame = 1 
Query:   76 KKDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKE 465
            K+DS++ +LK R   N+  + EN++ IL+R+K+D  D S     +  Y QAE D YEM+VR+AN+V+  E+L R+VSEIKQLL LGDF W+  TT    +E   +R  ++R    L+D +A DL AI++E
Sbjct:   18 KRDSHVQSLKTRLRKNMNAITENYELILSRAKVDTNDNSA---ILGAYAQAEQDTYEMSVRAANIVHACENLTRLVSEIKQLLILGDFCWLATTTATASEELRKRRADLDRVSTRLRDKLAADLYAIEEE 144
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. TrEMBL
Match: A0A0X3PZ72 (Mediator of RNA polymerase II transcription subunit 22 OS=Schistocephalus solidus OX=70667 GN=MED22 PE=4 SV=1)

HSP 1 Score: 107.842 bits (268), Expect = 1.930e-25
Identity = 59/136 (43.38%), Postives = 88/136 (64.71%), Query Frame = 1 
Query:   76 KKDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKELSNRVD 483
            K+++YL +LK R    ++ + ENF+ ILAR+K++    SD   ++NPYTQAE D +EMNVRS N+V   E++ R+VSEIKQLL LGDF W+E+ T+   +E   +R  ++     L D +A DL  +D +L   V+
Sbjct:   17 KRETYLNSLKSRLRDYLKTITENFELILARAKVE---NSDVIVRLNPYTQAEQDNFEMNVRSCNIVSACENITRLVSEIKQLLILGDFCWLEKVTKMGAEERERRRAELDAVAIKLADELAADLFTLDDKLGTTVN 149
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. TrEMBL
Match: A0A183SF49 (Uncharacterized protein OS=Schistocephalus solidus OX=70667 PE=4 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 1.034e-23
Identity = 57/131 (43.51%), Postives = 85/131 (64.89%), Query Frame = 1 
Query:   91 LYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKELSNRVD 483
            L +LK R    ++ + ENF+ ILAR+K+ +++ SD   ++NPYTQAE D +EMNVRS N+V   E++ R+VSEIKQLL LGDF W+E+ T+   +E   +R  ++     L D +A DL  +D +L   V+
Sbjct:    1 LNSLKSRLRDYLKTITENFELILARAKVTVEN-SDVIVRLNPYTQAEQDNFEMNVRSCNIVSACENITRLVSEIKQLLILGDFCWLEKVTKMGAEERERRRAELDAVAIKLADELAADLFTLDDKLGTTVN 130
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. TrEMBL
Match: A0A5J4NEQ7 (Mediator of RNA polymerase II transcription subunit 22 OS=Paragonimus westermani OX=34504 GN=DEA37_0013529 PE=4 SV=1)

HSP 1 Score: 103.99 bits (258), Expect = 1.038e-23
Identity = 55/141 (39.01%), Postives = 89/141 (63.12%), Query Frame = 1 
Query:   52 MARVTSSAKKDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKELSN 474
            M ++ ++ K+DS++ +LK R   N+  ++EN++ IL R+KID    +     +  Y QAE D +EM+VR+AN+V+  E+L R+VSEIKQLL LGDF W+ + T    ++   +R  ++R    L+D +A DL AI++E   
Sbjct:   18 MLQLNAARKRDSHVQSLKSRLRMNMNAIVENYESILLRTKIDASTDATGTTGLVSYAQAEQDTFEMSVRAANIVHACENLTRMVSEIKQLLILGDFCWLAQVTSTNCEDLQRRRMDLDRVSLRLRDKLAGDLYAIEEECGG 158
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Cavefish
Match: med22 (mediator complex subunit 22 [Source:NCBI gene;Acc:415234])

HSP 1 Score: 69.3218 bits (168), Expect = 1.944e-13
Identity = 40/123 (32.52%), Postives = 75/123 (60.98%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDL 447
            K++ L +  +R   +IR++L+NF  I+  +K++ +       +++  TQAE D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + E      ++    ++  ++ +  L+D +A+DL
Sbjct:  159 KETLLQSYNKRLKDDIRSILDNFTEIIKTAKVEDET------QVSRATQAEQDHYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAISLRNQQLRALQEECDKKLISLRDEIAVDL 275
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Nematostella
Match: EDO47193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RN15])

HSP 1 Score: 62.003 bits (149), Expect = 2.996e-12
Identity = 33/93 (35.48%), Postives = 58/93 (62.37%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEET 357
            K++ L +  +R   +++++L+NF  IL  SK++         ++   TQ+  D YE+NVR+AN+V   ESL+++VS+IK+ L L DF  + E+
Sbjct:   12 KETLLKSYTKRLKDDVKSILDNFTEILKCSKVE------EETQVARLTQSVQDQYEVNVRAANIVRAGESLMKLVSDIKEFLILNDFPSVNES 98
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Medaka
Match: med22 (mediator complex subunit 22 [Source:NCBI gene;Acc:101170849])

HSP 1 Score: 69.707 bits (169), Expect = 9.817e-15
Identity = 43/132 (32.58%), Postives = 77/132 (58.33%), Query Frame = 1 
Query:   79 KDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERC---ICDLQDNVAMDLEAIDKE 465
            K++ L N  +R   +I+++++NF  I+  +KI+         +++   QAE D YEM+VR+AN+V   ESL+++VS++KQ L L DF  + +    +  +N   R   E C   +  L+D +A+DL  +++E
Sbjct:   11 KETLLQNYNKRLKDDIKSIMDNFTEIIKTAKIE------EETQVSRPAQAEQDHYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNDA---ISLQNQQLRSLQEECDKKLMSLRDEIAVDLYELEEE 133
[Close]
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Planmine SMEST
Match: SMESG000029018.1 (SMESG000029018.1)

HSP 1 Score: 309.686 bits (792), Expect = 1.743e-108
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 1 
Query:   52 MARVTSSAKKDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKELSNRVDISFLFND 504
            MARVTSSAKKDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKELSNRVDISFLFND
Sbjct:    1 MARVTSSAKKDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRRDKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIEETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKELSNRVDISFLFND 151
The following BLAST results are available for this feature:
BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MED227.957e-1530.23mediator complex subunit 22 [Source:HGNC Symbol;Ac... [more]
MED227.957e-1530.23mediator complex subunit 22 [Source:HGNC Symbol;Ac... [more]
MED229.148e-1530.23mediator complex subunit 22 [Source:HGNC Symbol;Ac... [more]
MED229.148e-1530.23mediator complex subunit 22 [Source:HGNC Symbol;Ac... [more]
MED221.071e-1430.23mediator complex subunit 22 [Source:HGNC Symbol;Ac... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mdt-221.818e-625.00Mediator of RNA polymerase II transcription subuni... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MED221.391e-1332.58gene:FBgn0040339 transcript:FBtr0070165[more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
med221.137e-1534.85mediator complex subunit 22 [Source:NCBI gene;Acc:... [more]
med221.137e-1534.85mediator complex subunit 22 [Source:NCBI gene;Acc:... [more]
med224.493e-1534.09mediator complex subunit 22 [Source:NCBI gene;Acc:... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
shc12.863e-1431.97SHC (Src homology 2 domain containing) transformin... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Med224.714e-1531.01mediator complex subunit 22 [Source:MGI Symbol;Acc... [more]
Med227.881e-1431.15mediator complex subunit 22 [Source:MGI Symbol;Acc... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|A5WUL3|MED22_DANRE3.461e-1434.09Mediator of RNA polymerase II transcription subuni... [more]
sp|Q6GQ95|MED22_XENLA6.797e-1431.97Mediator of RNA polymerase II transcription subuni... [more]
sp|A0JPN6|MED22_RAT5.241e-1331.15Mediator of RNA polymerase II transcription subuni... [more]
sp|Q62276|MED22_MOUSE5.513e-1331.15Mediator of RNA polymerase II transcription subuni... [more]
sp|Q15528|MED22_HUMAN8.015e-1330.33Mediator of RNA polymerase II transcription subuni... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4E0RSD67.568e-2745.38Mediator of RNA polymerase II transcription subuni... [more]
A0A504YZ594.576e-2644.62Mediator of RNA polymerase II transcription subuni... [more]
A0A0X3PZ721.930e-2543.38Mediator of RNA polymerase II transcription subuni... [more]
A0A183SF491.034e-2343.51Uncharacterized protein OS=Schistocephalus solidus... [more]
A0A5J4NEQ71.038e-2339.01Mediator of RNA polymerase II transcription subuni... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
med221.944e-1332.52mediator complex subunit 22 [Source:NCBI gene;Acc:... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO471932.996e-1235.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
med229.817e-1532.58mediator complex subunit 22 [Source:NCBI gene;Acc:... [more]
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BLAST of Mediator of RNA polymerase II transcription subunit 22 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000029018.11.743e-108100.00SMESG000029018.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002318 ID=SMED30002318|Name=Mediator of RNA polymerase II transcription subunit 22|organism=Schmidtea mediterranea sexual|type=transcript|length=733bp
CTTTTACTAATATCAATGGACTATTTATATTGTTTAAATATATAGGGTTT
TATGGCGCGCGTTACCTCAAGTGCTAAGAAAGATTCTTATTTATACAATC
TTAAACAAAGATATAGTCATAATATTAGAAATTTACTTGAAAATTTCGAT
TACATTTTAGCACGATCTAAAATTGATATAAAAGATGGGAGCGATAGGCG
AGACAAAATAAATCCATATACTCAAGCAGAAATGGATGGTTACGAAATGA
ATGTCCGATCTGCTAACATGGTTTATCATGCTGAATCTTTATTGAGAATT
GTGTCTGAAATCAAACAATTATTAACACTAGGAGATTTCCAATGGATAGA
AGAAACGACAGAAAAAGTTAAAAAAGAAAACATAATGAAAAGACAAAGAA
TAGAGAGATGTATTTGTGATTTACAAGATAATGTTGCAATGGATTTAGAA
GCAATTGATAAGGAATTATCAAACAGAGTTGATATTAGTTTTCTTTTTAA
TGATTAACTTGTCCTTGATATGAATACAAGTGTATATATATTTGTATTAT
TATGTTTACATTAATTTGGTTACAAACTTTCATAAGTTCGATGTAATTGA
TATTTAAAGGATTAGGTCGAAAACGATTTATTTTAATAATTTCGACGAAA
TTAATTAAGTTAAAATATAAGGCTGTCATAATGAAAATCGTTTATTTGTC
GCTGCTAGTTAGCAAAGATCTATTACTTGTAAG
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protein sequence of SMED30002318-orf-1

>SMED30002318-orf-1 ID=SMED30002318-orf-1|Name=SMED30002318-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=152bp
MARVTSSAKKDSYLYNLKQRYSHNIRNLLENFDYILARSKIDIKDGSDRR
DKINPYTQAEMDGYEMNVRSANMVYHAESLLRIVSEIKQLLTLGDFQWIE
ETTEKVKKENIMKRQRIERCICDLQDNVAMDLEAIDKELSNRVDISFLFN
D*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0003712transcription cofactor activity
GO:0001104RNA polymerase II transcription cofactor activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR038427MED22_sf
IPR009332Med22
Vocabulary: cellular component
TermDefinition
GO:0016592mediator complex
Vocabulary: biological process
TermDefinition
GO:0006357regulation of transcription from RNA polymerase II promoter
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009332Mediator of RNA polymerase II transcription subunit 22PFAMPF06179Med22coord: 19..127
e-value: 4.0E-17
score: 62.3
IPR009332Mediator of RNA polymerase II transcription subunit 22PANTHERPTHR12434FAMILY NOT NAMEDcoord: 7..140
IPR038427MED22 superfamilyGENE3DG3DSA:1.20.58.1600coord: 8..144
e-value: 1.0E-17
score: 66.4