SMED30002314

Overview
NameSMED30002314
Smed IDSMED30002314
Length (bp)4670
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002314 (SMED30002314) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002314 (SMED30002314) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002314 (SMED30002314) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002314 (SMED30002314) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30002314

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30002314SMESG000049166.1 dd_Smed_v4_4059_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002314SMESG000049166.1 dd_Smed_v4_4059_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30002314SMESG000049166.1 dd_Smed_v4_3900_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30002314SMESG000049166.1 dd_Smed_v4_3900_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002314SMESG000049166.1 dd_Smed_v4_3900_1_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002314SMESG000049166.1 Contig28601uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002314SMESG000049166.1 Contig28601newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parenchymal cellSMED30002314SMESG000049166.1 dd_Smed_v6_3900_1dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pigment cellSMED30002314SMESG000049166.1 dd_Smed_v6_3900_1dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002314SMESG000049166.1 dd_Smed_v6_4059_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30002314SMESG000049166.1 dd_Smed_v4_3900_1_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30002314 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000535:34938..60045 +23153
Homology
BLAST of SMED30002314 vs. Planmine SMEST
Match: SMESG000049166.1 (SMESG000049166.1)

HSP 1 Score: 2396.31 bits (6209), Expect = 0.000e+0
Identity = 1418/1420 (99.86%), Postives = 1419/1420 (99.93%), Query Frame = 2
Query:  245 MTSIKDRLKLFEVNGXXXXXXXXXXXDIAPRNSSFTKSDINNELTNSQSEIKQENNLCRNSEILPSINPNDLCNESRHIVSVSQDSPPDIQGHSQTSNDNPLVTSVSNESAACLSVDCSRDRISEHQSDISDLNSNKEINLKANKDIGDDAMTDEDRIESYPSAYSIAGXXXXXXSSDKDSLYDFSYEYQGSIYHLPGLENFIFPIDREGSTELPTVSTFDHPDSRRPHQESPPGSGLPTIEIAKNILEDEPDVSEVANNLKRTASKRLIEELFNTSVWRNSVNYSDNFIFNPATTTESSVSSLRITDXXXXXXXXXXXXXXDEXXXXXXXXXXXXPNIRTLFSGMFRSKRGHKKSHEKISGQDQNNDIYDSDGHDKAANNSKMVEGDSDSVEQVTKKPLNSIFNRDWLNSLGEGIKKKANVMSELKKVFTHGXXXXXXXXXXXXXXXXXXVEAHRANIDKEKMXXXXXXXXXXXXXNSSAEINYPKTILPVQKSVDKIKLNNQRNSYKTKIQNNVTLKETGDKVDEIYDDDGNGSDSVYDDGGEDEDKVSTKSSCNESVYDDGDDGGIENANNLDINSCSKYSSHESVYDDGGEELQISSAQSDNSPQVSDVEMRPKKINKLINNPIFNLTIPLKTPHEIYVQKHFVKDVAKGSIAKAIEVNDSIXXXXXXXXXXXTNLNSNGKTNKVDEKKHKEFTDDNRSQQRSEVSSFGETKSEKKTNQSNNVKKSHKNTSDFRWLFPXXXXXXXXXXXXXXXXXXLKEAPSKIKTDKGKNKTNPPPQSKSLHQXXXXXXXXXXXXXXXXXXSKNELKDNVEEDMYDMDSDEIPKRMSNDKGVENSKPIMHQIFGNLKNPGKLPETNNKNQKTFDLVSNXXXXXXXXXXXXXXXXXXXIHKKDRIPTNKVNKTVLEEYQTEFDKHVKARNVSKEIIKGNQRVTDISKKILPTNKKKNVDEEYDEDALXXXXXXXXXXXXXXXXXXXTILQKSKTIPETIXXXXXXXXXXXXXXXXEKNSIFSGKPKKADNXXXXXXXXXXXXXXXXXYIYDEDAPNENEVYVSDVEIKEENKTDLPKFKTATETIKSKKVDDNNKLTIKTNEKTSIFSGKLKKVAKENKTKKSNGKSKPETYIYDEDTPNESENEVYVSDEEIRAEKEINLPTSKTIPETIKSKKVDDNNKLTIKTNEKTSIFSGKLKKVAKENKTKKSNGKSKPETYIYDEDTPNESENEVYVSDEEIRAEKEINLPTSKTIPETIKSKNEIDNKKLEIKKNEKTSIFSVKSKKAADDKERKKIKKSACKTKPETLIYDEDASENLGNEVQDYNYLDDEFFNTEDPDINKIIKKNLNQAKKVFKLNDNLMPVSLSKAVKTVYSQRKNHIQLNTGELVQVLEYKDDLALVRNAVGKCGWTTPGSLQIIKLYRDQQC 4504
            MTSIKDRLKLFEVNGPPVKPKPKAKPDIAPRNSSFTKSDINNELTNSQSEIKQENNLCRNSEILPSINPNDLCNESRHIVSVSQDSPPDIQGHSQTSNDNPLVTSVSNESAACLSVDCSRDRISEHQSDISDLNSNKEINLKANKDIGDDAMTDEDRIESYPSAYSIAGEEEVEESSDKDSLYDFSYEYQGSIYHLPGLENFIFPIDREGSTELPTVSTFDHPDSRRPHQESPPGSGLPTIEIAKNILEDEPDVSEVANNLKRTASKRLIEELFNTSVWRNSVNYSDNFIFNPATTTESSVSSLRITDISSINSINSSSPSNDERRKSTTSLSSSKPNIRTLFSGMFRSKRGHKKSHEKISGQDQNNDIYDSDGHDKAANNSKMVEGDSDSVEQVTKKPLNSIFNRDWLNSLGEGIKKKANVMSELKKVFTHGKTKHLKKKPKKSISVASSVEAHRANIDKEKMIEDEDIYDDGGDDNSSAEINYPKTILPVQKSVDKIKLNNQRNSYKTKIQNNVTLKETGDKVDEIYDDDGNGSDSVYDDGGEDEDKVSTKSSCNESVYDDGDDGGIENANNLDINSCSKYSSHESVYDDGGEELQISSAQSDNSPQVSDVEMRPKKINKLINNPIFNLTIPLKTPHEIYVQKHFVKDVAKGSIAKAIEVNDSIYDDGGEDYEEDTNLNSNGKTNKVDEKKHKEFTDDNRSQQRSEVSSFGETKSEKKTNQSNNVKKSHKNTSDFRWLFPKKSKSKTKSETTKDNEKKLKEAPSKIKTDKGKNKTNPPPQSKSLHQNEEYDNDDENLEEHNEPESKNELKDNVEEDMYDMDSDEIPKRMSNDKGVENSKPIMHQIFGNLKNPGKLPETNNKNQKTFDLVSNEKEDKTVSKKSKKPHKTSEIHKKDRIPTNKVNKTVLEEYQTEFDKHVKARNVSKEIIKGNQRVTDISKKILPTNKKKNVDEEYDEDALDENENEVYVLDEVDEEEKETILQKSKTIPETIKLKKDDDNNKLARKKNEKNSIFSGKPKKADNAKESKTKKSSAKSKPETYIYDEDAPNENEVYVSDVEIKEENKTDLPKFKTATETIKSKKVDDNNKLTIKTNEKTSIFSGKLKKVAKE KTKKSNGKSKPETYIYDEDTPNESENEVYVSDEEIRAEKEINLPTSKTIPETIKSKKVDDNNKLTIKTNEKT+IFSGKLKKVAKENKTKKSNGKSKPETYIYDEDTPNESENEVYVSDEEIRAEKEINLPTSKTIPETIKSKNEIDNKKLEIKKNEKTSIFSVKSKKAADDKERKKIKKSACKTKPETLIYDEDASENLGNEVQDYNYLDDEFFNTEDPDINKIIKKNLNQAKKVFKLNDNLMPVSLSKAVKTVYSQRKNHIQLNTGELVQVLEYKDDLALVRNAVGKCGWTTPGSLQIIKLYRDQQC
Sbjct:    1 MTSIKDRLKLFEVNGPPVKPKPKAKPDIAPRNSSFTKSDINNELTNSQSEIKQENNLCRNSEILPSINPNDLCNESRHIVSVSQDSPPDIQGHSQTSNDNPLVTSVSNESAACLSVDCSRDRISEHQSDISDLNSNKEINLKANKDIGDDAMTDEDRIESYPSAYSIAGEEEVEESSDKDSLYDFSYEYQGSIYHLPGLENFIFPIDREGSTELPTVSTFDHPDSRRPHQESPPGSGLPTIEIAKNILEDEPDVSEVANNLKRTASKRLIEELFNTSVWRNSVNYSDNFIFNPATTTESSVSSLRITDISSINSINSSSPSNDERRKSTTSLSSSKPNIRTLFSGMFRSKRGHKKSHEKISGQDQNNDIYDSDGHDKAANNSKMVEGDSDSVEQVTKKPLNSIFNRDWLNSLGEGIKKKANVMSELKKVFTHGKTKHLKKKPKKSISVASSVEAHRANIDKEKMIEDEDIYDDGGDDNSSAEINYPKTILPVQKSVDKIKLNNQRNSYKTKIQNNVTLKETGDKVDEIYDDDGNGSDSVYDDGGEDEDKVSTKSSCNESVYDDGDDGGIENANNLDINSCSKYSSHESVYDDGGEELQISSAQSDNSPQVSDVEMRPKKINKLINNPIFNLTIPLKTPHEIYVQKHFVKDVAKGSIAKAIEVNDSIYDDGGEDYEEDTNLNSNGKTNKVDEKKHKEFTDDNRSQQRSEVSSFGETKSEKKTNQSNNVKKSHKNTSDFRWLFPKKSKSKTKSETTKDNEKKLKEAPSKIKTDKGKNKTNPPPQSKSLHQNEEYDNDDENLEEHNEPESKNELKDNVEEDMYDMDSDEIPKRMSNDKGVENSKPIMHQIFGNLKNPGKLPETNNKNQKTFDLVSNEKEDKTVSKKSKKPHKTSEIHKKDRIPTNKVNKTVLEEYQTEFDKHVKARNVSKEIIKGNQRVTDISKKILPTNKKKNVDEEYDEDALDENENEVYVLDEVDEEEKETILQKSKTIPETIKLKKDDDNNKLARKKNEKNSIFSGKPKKADNAKESKTKKSSAKSKPETYIYDEDAPNENEVYVSDVEIKEENKTDLPKFKTATETIKSKKVDDNNKLTIKTNEKTSIFSGKLKKVAKEYKTKKSNGKSKPETYIYDEDTPNESENEVYVSDEEIRAEKEINLPTSKTIPETIKSKKVDDNNKLTIKTNEKTNIFSGKLKKVAKENKTKKSNGKSKPETYIYDEDTPNESENEVYVSDEEIRAEKEINLPTSKTIPETIKSKNEIDNKKLEIKKNEKTSIFSVKSKKAADDKERKKIKKSACKTKPETLIYDEDASENLGNEVQDYNYLDDEFFNTEDPDINKIIKKNLNQAKKVFKLNDNLMPVSLSKAVKTVYSQRKNHIQLNTGELVQVLEYKDDLALVRNAVGKCGWTTPGSLQIIKLYRDQQC 1420          
The following BLAST results are available for this feature:
BLAST of SMED30002314 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002314 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000049166.10.000e+099.86SMESG000049166.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002314 ID=SMED30002314|Name=SMED30002314|organism=Schmidtea mediterranea sexual|type=transcript|length=4670bp
ACCGCATACACACTGAGTGCGTGCTAACATGCATGCAAGATGACTAATGC
TAGTCATGCAGTACATGCAGTATTATTCAAAGACACTACAATGCCATAAT
TCTGCAACCAATCAAATTGCGATATTTACAAGTTTGTTAATCAATGTCTC
AGCCGTGTTCAGTTTCATATTGATTTAAATTATTTCATATTAAATTATTC
AATGATTTATTAAAATTCTGTTATTTTGGAGTTAAATTTTCAAGATGACA
TCAATTAAAGATCGATTGAAATTATTTGAAGTAAACGGTCCACCTGTTAA
GCCAAAACCGAAAGCCAAACCAGATATTGCTCCAAGAAATTCATCATTCA
CTAAATCAGATATTAACAACGAACTCACCAATTCCCAGTCGGAAATAAAA
CAAGAGAATAATTTATGCAGGAACTCTGAAATTTTACCCTCAATTAATCC
AAACGACCTCTGTAATGAATCAAGGCATATTGTCAGTGTAAGCCAAGACA
GTCCGCCTGATATTCAGGGACATTCACAGACTTCTAATGATAATCCGTTA
GTAACCTCCGTCAGCAACGAGAGTGCCGCATGTCTATCAGTTGATTGCAG
CAGAGATCGTATCAGTGAACACCAAAGTGATATATCTGATTTAAATTCAA
ACAAAGAAATCAATCTCAAAGCGAACAAAGATATTGGTGATGACGCAATG
ACTGATGAAGATCGAATTGAATCGTATCCAAGCGCGTATTCTATAGCTGG
CGAAGAAGAGGTTGAGGAATCCTCCGATAAAGATTCGCTCTATGATTTCA
GTTACGAATATCAGGGATCAATTTATCATTTACCGGGATTGGAGAATTTC
ATATTTCCCATCGATCGTGAAGGATCAACTGAGCTGCCGACTGTCAGTAC
GTTTGATCATCCTGATTCCAGGCGCCCTCACCAGGAAAGCCCTCCCGGTA
GTGGGCTGCCGACAATTGAAATTGCAAAGAATATCCTGGAGGACGAGCCG
GATGTGTCCGAGGTGGCAAACAACTTGAAACGGACTGCATCCAAACGGTT
GATAGAGGAATTGTTCAACACGTCCGTCTGGCGGAATTCCGTCAATTACA
GTGACAATTTCATATTCAATCCAGCCACGACAACCGAATCGAGCGTTAGT
TCGCTCAGAATCACAGACATCAGCTCAATTAATTCCATAAATTCCTCCAG
TCCGTCGAATGATGAGCGCAGAAAGTCAACTACCTCGCTTTCATCCTCGA
AACCGAACATTAGGACCCTGTTCAGTGGAATGTTCCGGTCGAAGCGAGGG
CATAAAAAATCCCATGAAAAGATTTCGGGCCAAGATCAAAATAATGATAT
CTACGATTCCGACGGACACGACAAGGCAGCCAATAACAGCAAAATGGTGG
AAGGGGATTCCGATTCCGTCGAGCAAGTCACAAAAAAGCCTCTGAATTCC
ATTTTCAACCGAGATTGGTTGAACAGTTTAGGCGAAGGCATCAAGAAGAA
AGCAAATGTTATGTCCGAATTAAAGAAAGTTTTCACTCATGGTAAAACAA
AACACCTCAAAAAAAAACCAAAGAAAAGCATATCAGTTGCCTCCTCAGTC
GAAGCGCACAGAGCAAATATTGATAAAGAGAAAATGATTGAAGATGAGGA
TATTTATGATGATGGCGGGGACGACAATTCATCCGCGGAAATAAATTATC
CTAAAACAATACTGCCAGTACAGAAATCGGTTGACAAAATAAAACTTAAC
AACCAAAGAAATTCCTATAAAACAAAAATTCAAAACAATGTAACCTTGAA
GGAAACTGGAGATAAAGTGGATGAGATTTATGATGATGATGGTAATGGGA
GTGATAGTGTCTATGATGATGGGGGAGAGGATGAAGACAAAGTAAGTACA
AAAAGCTCATGCAATGAGAGTGTTTATGATGATGGAGATGACGGAGGAAT
AGAGAATGCAAATAATCTTGATATAAATTCGTGCTCAAAATATTCAAGTC
ACGAAAGTGTTTATGATGATGGAGGCGAAGAATTACAAATCTCCTCAGCC
CAATCGGATAATTCACCTCAAGTTTCTGATGTTGAAATGCGTCCTAAGAA
GATAAATAAATTAATAAATAATCCTATATTTAATTTAACTATTCCCTTAA
AGACACCTCACGAAATCTATGTCCAAAAGCATTTCGTAAAGGACGTTGCT
AAAGGTTCAATTGCTAAAGCAATAGAAGTTAATGACAGCATTTACGATGA
TGGAGGAGAAGATTATGAAGAGGACACAAATTTAAATTCAAATGGAAAAA
CCAATAAAGTAGATGAAAAGAAACACAAAGAGTTTACAGATGACAATCGC
TCTCAACAAAGATCCGAAGTTTCTAGTTTCGGAGAAACTAAATCGGAAAA
GAAAACTAACCAATCAAATAACGTTAAAAAATCGCATAAAAACACTTCTG
ATTTCAGATGGTTATTCCCGAAAAAATCAAAGAGTAAAACAAAGTCTGAA
ACGACAAAAGATAATGAAAAGAAATTGAAAGAAGCTCCTTCGAAGATTAA
AACGGATAAAGGTAAGAACAAAACAAATCCTCCCCCGCAATCTAAATCTT
TACATCAAAATGAGGAATATGACAATGATGATGAAAATCTTGAGGAGCAT
AATGAACCTGAAAGTAAGAATGAATTAAAGGATAATGTAGAGGAAGATAT
GTATGATATGGATTCGGACGAGATTCCAAAGCGTATGTCAAATGATAAGG
GCGTAGAAAATAGTAAACCAATAATGCATCAAATTTTTGGAAATTTGAAG
AATCCGGGGAAGTTACCGGAAACAAATAACAAAAATCAAAAAACCTTCGA
CTTAGTGAGCAACGAAAAGGAGGATAAAACGGTCAGTAAGAAGTCTAAAA
AACCTCATAAAACATCTGAAATTCACAAAAAAGATAGGATACCGACCAAT
AAAGTTAATAAAACAGTTTTAGAAGAATATCAAACAGAGTTTGACAAGCA
TGTAAAGGCCAGAAATGTATCGAAAGAAATCATAAAAGGAAATCAGAGAG
TCACGGATATATCAAAAAAGATTTTACCAACTAATAAAAAGAAAAATGTT
GATGAAGAATATGATGAAGATGCTCTTGATGAAAATGAAAATGAAGTGTA
TGTTTTAGACGAAGTAGATGAGGAAGAAAAGGAAACAATTTTACAAAAAT
CCAAAACCATTCCCGAGACTATCAAATTAAAAAAGGATGACGACAATAAC
AAGTTAGCAAGAAAAAAAAATGAAAAGAATAGTATATTTTCTGGGAAACC
GAAAAAGGCTGACAATGCTAAAGAAAGTAAAACTAAGAAATCATCGGCCA
AATCAAAGCCGGAAACATATATTTACGATGAAGATGCACCAAATGAAAAC
GAAGTTTATGTATCCGATGTAGAAATAAAAGAGGAAAACAAGACCGATTT
ACCAAAATTCAAAACTGCCACGGAGACTATCAAATCAAAAAAGGTAGACG
ACAATAACAAGTTAACGATAAAAACAAATGAAAAGACAAGTATATTTTCG
GGGAAATTGAAAAAGGTTGCAAAGGAAAACAAAACAAAGAAGTCCAATGG
CAAGTCAAAACCCGAGACATATATTTACGATGAGGATACACCCAATGAAA
GTGAAAATGAAGTTTACGTTTCCGATGAAGAAATAAGGGCAGAGAAGGAG
ATTAATTTACCGACATCCAAAACCATTCCAGAGACTATCAAATCAAAAAA
GGTAGACGACAATAACAAGTTAACGATAAAAACAAATGAAAAGACAAGTA
TATTTTCGGGGAAATTGAAAAAGGTTGCAAAGGAAAACAAAACAAAGAAG
TCCAATGGCAAGTCAAAACCCGAGACATATATTTACGATGAGGATACACC
CAATGAAAGTGAAAATGAAGTTTACGTTTCCGATGAAGAAATAAGGGCAG
AGAAGGAGATTAATTTACCGACATCCAAAACCATTCCAGAGACTATCAAA
TCAAAAAATGAAATTGACAATAAAAAGTTAGAAATAAAAAAAAATGAAAA
AACAAGTATATTTTCTGTTAAATCGAAAAAAGCTGCCGATGATAAGGAAA
GAAAAAAAATAAAAAAGTCCGCTTGCAAGACAAAACCTGAAACTTTAATT
TATGACGAGGACGCTTCAGAAAATCTGGGCAATGAAGTACAAGATTATAA
CTATCTCGATGATGAATTTTTCAACACGGAGGATCCCGACATCAACAAAA
TTATAAAGAAAAATTTGAATCAGGCTAAAAAAGTATTCAAACTGAACGAC
AACCTCATGCCAGTTTCTCTTTCAAAGGCTGTCAAAACTGTTTATTCCCA
GCGCAAAAATCACATTCAATTAAACACTGGGGAATTGGTACAAGTTCTCG
AGTACAAGGATGATCTAGCCTTGGTCAGAAATGCAGTGGGGAAATGTGGG
TGGACTACACCAGGAAGCCTGCAGATCATCAAATTATACAGGGACCAGCA
GTGTTGACCACCATCAATATTACCTTATAAATTCATTATGACAATTATTT
AAATACATCTTATATTGACTTTGTATCACTAGCACTTATATTGATTATCA
ATTGTGCTAGCACTAATCAATAATTCTATTATAGTGCTAATATAATGGCA
GCGGCTCTCTTCTAAAAAAA
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protein sequence of SMED30002314-orf-1

>SMED30002314-orf-1 ID=SMED30002314-orf-1|Name=SMED30002314-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1421bp
MTSIKDRLKLFEVNGPPVKPKPKAKPDIAPRNSSFTKSDINNELTNSQSE
IKQENNLCRNSEILPSINPNDLCNESRHIVSVSQDSPPDIQGHSQTSNDN
PLVTSVSNESAACLSVDCSRDRISEHQSDISDLNSNKEINLKANKDIGDD
AMTDEDRIESYPSAYSIAGEEEVEESSDKDSLYDFSYEYQGSIYHLPGLE
NFIFPIDREGSTELPTVSTFDHPDSRRPHQESPPGSGLPTIEIAKNILED
EPDVSEVANNLKRTASKRLIEELFNTSVWRNSVNYSDNFIFNPATTTESS
VSSLRITDISSINSINSSSPSNDERRKSTTSLSSSKPNIRTLFSGMFRSK
RGHKKSHEKISGQDQNNDIYDSDGHDKAANNSKMVEGDSDSVEQVTKKPL
NSIFNRDWLNSLGEGIKKKANVMSELKKVFTHGKTKHLKKKPKKSISVAS
SVEAHRANIDKEKMIEDEDIYDDGGDDNSSAEINYPKTILPVQKSVDKIK
LNNQRNSYKTKIQNNVTLKETGDKVDEIYDDDGNGSDSVYDDGGEDEDKV
STKSSCNESVYDDGDDGGIENANNLDINSCSKYSSHESVYDDGGEELQIS
SAQSDNSPQVSDVEMRPKKINKLINNPIFNLTIPLKTPHEIYVQKHFVKD
VAKGSIAKAIEVNDSIYDDGGEDYEEDTNLNSNGKTNKVDEKKHKEFTDD
NRSQQRSEVSSFGETKSEKKTNQSNNVKKSHKNTSDFRWLFPKKSKSKTK
SETTKDNEKKLKEAPSKIKTDKGKNKTNPPPQSKSLHQNEEYDNDDENLE
EHNEPESKNELKDNVEEDMYDMDSDEIPKRMSNDKGVENSKPIMHQIFGN
LKNPGKLPETNNKNQKTFDLVSNEKEDKTVSKKSKKPHKTSEIHKKDRIP
TNKVNKTVLEEYQTEFDKHVKARNVSKEIIKGNQRVTDISKKILPTNKKK
NVDEEYDEDALDENENEVYVLDEVDEEEKETILQKSKTIPETIKLKKDDD
NNKLARKKNEKNSIFSGKPKKADNAKESKTKKSSAKSKPETYIYDEDAPN
ENEVYVSDVEIKEENKTDLPKFKTATETIKSKKVDDNNKLTIKTNEKTSI
FSGKLKKVAKENKTKKSNGKSKPETYIYDEDTPNESENEVYVSDEEIRAE
KEINLPTSKTIPETIKSKKVDDNNKLTIKTNEKTSIFSGKLKKVAKENKT
KKSNGKSKPETYIYDEDTPNESENEVYVSDEEIRAEKEINLPTSKTIPET
IKSKNEIDNKKLEIKKNEKTSIFSVKSKKAADDKERKKIKKSACKTKPET
LIYDEDASENLGNEVQDYNYLDDEFFNTEDPDINKIIKKNLNQAKKVFKL
NDNLMPVSLSKAVKTVYSQRKNHIQLNTGELVQVLEYKDDLALVRNAVGK
CGWTTPGSLQIIKLYRDQQC*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0002075pigment cell
PLANA:0003116parenchymal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 37..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 345..370
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 686..703
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 704..720
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 219..238
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 31..53
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 869..883
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 523..569
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 994..1049
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 316..335
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 721..774
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..100
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 801..815
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1195..1229
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1113..1143
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..53
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1113..1129
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1195..1215
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 852..904
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 994..1045
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 673..838