SMED30002307

Overview
NameSMED30002307
Smed IDSMED30002307
Length (bp)3640
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002307 (SMED30002307) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002307 (SMED30002307) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002307 (SMED30002307) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002307 (SMED30002307) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002307

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30002307SMESG000036134.1 SmedASXL_007690SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30002307SMESG000036134.1 SMESG000019910.1 SmedASXL_016345SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X2 cellSMED30002307SMESG000036134.1 SMESG000019910.1 SmedASXL_016345SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30002307SMESG000036134.1 SmedASXL_000259SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30002307 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000901:2245..7978 +13943
Homology
[Close]
BLAST of SMED30002307 vs. TrEMBL
Match: A0A4S2LZE2 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005180 PE=4 SV=1)

HSP 1 Score: 70.0922 bits (170), Expect = 3.621e-8
Identity = 76/286 (26.57%), Postives = 129/286 (45.10%), Query Frame = 2 
Query:   98 SLQNFIKKASDVNLNTAIAMSKSLKDLEKQNRKKAK---ELYLT----PFHLMKDPIEVKKVLDLRLSNIILQSFQ--------INLIDIVPRHTCKLWEKASLYCDIKPGDLSYDNINLSELMDYYVSALIPYIIPSTYREGDKMLNVSQIVGRERSIIYSNKNESSYQSPHKEV--TNDTFNVIEI----DDSI---QLSSKSPEIANVTESEIINEDLNHFSPTNDWENSNLFPENISATPENDLSQCRFLEMFNKDKFSDFIVFLKSETVLYLHKFVLAAWN 883
            +L+N      D +L TA+A+S S+ D +K+ R +A+   ++ LT    P HL+    + + +L  RL+ ++LQ+ +        +N   + P     LW  A+       GD+       S  + YYV AL+P + P+T   G+ +L++SQ+ GR      S+ + +   SP   +  T+ TF+  +     DDS+   Q S K+P   +      I +D                 E      EN +   R+L M  +  +SD ++ L     + +HKFV AAW 
Sbjct:   91 TLKNKTAGNVDDDLQTALALSLSVNDEDKRRRSEAEASSKVPLTELGPPSHLLLTKQQRQAILSDRLAALLLQAHRPIGNAITSMNDSSLPP-----LWVMAA-------GDVGGYVSESSPNVPYYVKALVPPLSPTTITWGENLLSLSQLPGRS-----SSHSPAHVPSPPNALLSTDPTFSQTQGFILDDDSMDCPQPSFKNPVCPHSPILIKIGDDSGS--------------EKSKPPSENPVYGNRYLSMVGESSWSDAVLILDDGQRVPVHKFVFAAWG 345
[Close]
BLAST of SMED30002307 vs. TrEMBL
Match: A0A4S2LY27 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005180 PE=4 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 4.009e-8
Identity = 76/286 (26.57%), Postives = 129/286 (45.10%), Query Frame = 2 
Query:   98 SLQNFIKKASDVNLNTAIAMSKSLKDLEKQNRKKAK---ELYLT----PFHLMKDPIEVKKVLDLRLSNIILQSFQ--------INLIDIVPRHTCKLWEKASLYCDIKPGDLSYDNINLSELMDYYVSALIPYIIPSTYREGDKMLNVSQIVGRERSIIYSNKNESSYQSPHKEV--TNDTFNVIEI----DDSI---QLSSKSPEIANVTESEIINEDLNHFSPTNDWENSNLFPENISATPENDLSQCRFLEMFNKDKFSDFIVFLKSETVLYLHKFVLAAWN 883
            +L+N      D +L TA+A+S S+ D +K+ R +A+   ++ LT    P HL+    + + +L  RL+ ++LQ+ +        +N   + P     LW  A+       GD+       S  + YYV AL+P + P+T   G+ +L++SQ+ GR      S+ + +   SP   +  T+ TF+  +     DDS+   Q S K+P   +      I +D                 E      EN +   R+L M  +  +SD ++ L     + +HKFV AAW 
Sbjct:   91 TLKNKTAGNVDDDLQTALALSLSVNDEDKRRRSEAEASSKVPLTELGPPSHLLLTKQQRQAILSDRLAALLLQAHRPIGNAITSMNDSSLPP-----LWVMAA-------GDVGGYVSESSPNVPYYVKALVPPLSPTTITWGENLLSLSQLPGRS-----SSHSPAHVPSPPNALLSTDPTFSQTQGFILDDDSMDCPQPSFKNPVCPHSPILIKIGDDSGS--------------EKSKPPSENPVYGNRYLSMVGESSWSDAVLILDDGQRVPVHKFVFAAWG 345
[Close]
BLAST of SMED30002307 vs. TrEMBL
Match: A0A4S2LZ01 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_005180 PE=4 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 5.285e-8
Identity = 76/286 (26.57%), Postives = 129/286 (45.10%), Query Frame = 2 
Query:   98 SLQNFIKKASDVNLNTAIAMSKSLKDLEKQNRKKAK---ELYLT----PFHLMKDPIEVKKVLDLRLSNIILQSFQ--------INLIDIVPRHTCKLWEKASLYCDIKPGDLSYDNINLSELMDYYVSALIPYIIPSTYREGDKMLNVSQIVGRERSIIYSNKNESSYQSPHKEV--TNDTFNVIEI----DDSI---QLSSKSPEIANVTESEIINEDLNHFSPTNDWENSNLFPENISATPENDLSQCRFLEMFNKDKFSDFIVFLKSETVLYLHKFVLAAWN 883
            +L+N      D +L TA+A+S S+ D +K+ R +A+   ++ LT    P HL+    + + +L  RL+ ++LQ+ +        +N   + P     LW  A+       GD+       S  + YYV AL+P + P+T   G+ +L++SQ+ GR      S+ + +   SP   +  T+ TF+  +     DDS+   Q S K+P   +      I +D                 E      EN +   R+L M  +  +SD ++ L     + +HKFV AAW 
Sbjct:   91 TLKNKTAGNVDDDLQTALALSLSVNDEDKRRRSEAEASSKVPLTELGPPSHLLLTKQQRQAILSDRLAALLLQAHRPIGNAITSMNDSSLPP-----LWVMAA-------GDVGGYVSESSPNVPYYVKALVPPLSPTTITWGENLLSLSQLPGRS-----SSHSPAHVPSPPNALLSTDPTFSQTQGFILDDDSMDCPQPSFKNPVCPHSPILIKIGDDSGS--------------EKSKPPSENPVYGNRYLSMVGESSWSDAVLILDDGQRVPVHKFVFAAWG 345
[Close]
BLAST of SMED30002307 vs. TrEMBL
Match: A0A5K4EQD8 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 63.5438 bits (153), Expect = 3.607e-6
Identity = 88/358 (24.58%), Postives = 158/358 (44.13%), Query Frame = 2 
Query:   71 LIDIMTKKKSLQNFIKKASDVNLNTAIAMSKSLKDLEKQNRKKAKELYLT---------PFHLMKDPIEVKKVLDLRLSNIILQSFQINLID-----------IVPRHTCKLWEKASLYCDIKPGDLSYDNINLSELMDYYVSALIPYIIPSTYREGDKMLNVSQIVGRERSIIYSNKNESSYQSPHKEVTNDTFNVIEID----DSIQLSSK-----SPEIANVTESEIINEDLNHFSPTNDWENSNLFPENISATPENDLSQCRFLEMFNKDKFSDFIVFLKSETVLYLHKFVLAAWNCNAEYLQDSQQFILNIDDEIVIKLLKHLYSD--GKFNIDDIIDSDDCLA-LCKIFGLN 1048
            L  +M+       FI++ ++ +L+TA A+S S+ +  KQ  +K++ +  T         P HL+    + +++L  +L +I+L+  +IN ++                   LW  AS           +D  +++++  YYV+ LIP + P+       ++++SQI GR               S HK++ +++ NV  +D    D    S K     SP  +N  E +    +L+  SP +     + +  NI++ P         L M      SD  + L    ++  HKF+ AAWN N  Y Q     + N+  + +I LL+ LYS    K  I+ I  + + L  + + +GLN
Sbjct:   72 LFSLMSLSCRDHRFIRRENE-DLHTACALSLSIDEEMKQ--RKSEAILATKIDPIDLDSPSHLLLSSEQRERILAEKLESILLEVKRINKLEKEYSNTTTNNNSNSSVQSNLWNLASTKL--------HDVDSITQM--YYVNKLIPPLSPTLNSLNSNIISMSQIPGR--------------VSIHKKMKSES-NVTTMDEYKEDHCDFSEKECKQLSPRQSNKPEDKCSTSELSSVSP-DTLSKCSSYLTNINSNP--------LLSMIGNSLCSDLCLILDEGDIIPAHKFIFAAWNLNINYSQCDIIEVRNVSKDELINLLQILYSGDRSKLTIEYIDHASEALQNIIRNWGLN 392
[Close]
BLAST of SMED30002307 vs. Planmine SMEST
Match: SMESG000036134.1 (SMESG000036134.1)

HSP 1 Score: 1681 bits (4352), Expect = 0.000e+0
Identity = 907/911 (99.56%), Postives = 909/911 (99.78%), Query Frame = 2 
Query:   83 MTKKKSLQNFIKKASDVNLNTAIAMSKSLKDLEKQNRKKAKELYLTPFHLMKDPIEVKKVLDLRLSNIILQSFQINLIDIVPRHTCKLWEKASLYCDIKPGDLSYDNINLSELMDYYVSALIPYIIPSTYREGDKMLNVSQIVGRERSIIYSNKNESSYQSPHKEVTNDTFNVIEIDDSIQLSSKSPEIANVTESEIINEDLNHFSPTNDWENSNLFPENISATPENDLSQCRFLEMFNKDKFSDFIVFLKSETVLYLHKFVLAAWNCNAEYLQDSQQFILNIDDEIVIKLLKHLYSDGKFNIDDIIDSDDCLALCKIFGLNRSLLQKHSLPVPFEPLCKSINTNQIISSNEKELKSLSAIKLPENNCDFSIDNILSKSIINLAQSTPNRFTLTNKTSEVSIKRSSNYSEKDLFESYDIDFDETTNLVVNNTLEAISNPSVQKIEEPLNNYSLHKKLCSFTNEPYLTNYPDTLTKLSYNNIDQPPDKRNNMEDVLTALPAQPEKNHIIQLSDRNPKIIEENFLSPTFCKMPNNNRISNNEIMKVTEPDLIIPSRVGDSINSEMCEENSDRLFDEIYYQSPISMTFGEEINFNHRNETEITKNNYLQHNSILTVKDNIILEDSPLPLRQRLGLKSLNSDINAVINISSDEELTNHEVKKPNKRSRDKIETIQSNSRFSTENGQEIKPKRSKTKSITDKLQRKPKKTLSEINIENXXXXXXXXXXXXXXXXXXXXTLNGFITPIKQQFLADPSFTPVPDFSAMSTSVIKGKCMNFGIKPNLPKNYKIKILEKVHIYERIKXXXXXXXXXXXXXXXXFXXXXXXXXXXXXXXXVVDNLNDNIQLYNFLKATYFRDILTYTPLDIKKVLAELKENNIKTSLKKLNDYFDKQCILHSSEDNKKGTNFSRPIKASKM 2815
            MTKKKSLQNFIKKASDVNLNTAIAMSKSLKDLEKQNRKKAKELYLTPFHLMKDPIEVKKVLDLRLSNIILQSFQINLIDIVPRHTCKLW+KASLYCDIKPGDLSYDNINLSELMDYYVSALIPYIIPSTYREGDKMLNVSQIVGRERSIIYSNKNESSYQSPHKEVTNDTFNVIEIDDSIQLSSKSPEIANVTESEIINEDLNHFSPTNDWENSNLFPENISATPENDLSQCRFLEMFNKDKFSDFIVFLKSETVLYLHKFVLAAWNCN EYLQDSQQFILNIDDEIVIKLLKHLYSDGKFNIDDIIDSDDCLALCKIFGLNRSLLQKHSLPVPFEPLCKSINTNQIISSNEKELKSLSAIKLPENNCDFSIDNILSKSIINLAQSTPNRFTLTNKTSEVSIKRSSNYSEKDLFESYDIDFDETTNLVVNNTLEAISNPSVQKIEEPLNNYSLHKKLCSFTNEPYLTNYPDTLTKLS+NNIDQPPDKRNNMEDVLTALPAQPEKNHIIQLSDRNPKIIEENFLSPTFCKMPNNNRISNNEIMKVTEPDLIIPSRVGDSINSEMCEENSDRLFDEIYYQSPISMTFGEEINFNHRNETEITKNNYLQHNSILTVKDNIILEDSPLPLRQRLGLKSLNSDINAVINISSDEELTNHEVKKPNKRSRDKIETIQSNSRFSTENGQEIKPKRSKTKSITDKLQRKPKKTLSEINIENVPKEPKVKKDKKSRKRKSSETLNGFITPIKQQFLADPSFTPVPDFSAMSTSVIKGKCMNFGIKPNLPKNYKIKILEKVHIYERIKNHSLSSSSNSDSTNVSFKENEESESNDSKDENV DNLNDNIQLYNFLKATYFRDILTYTPLDIKKVLAELKENNIKTSLKKLNDYFDKQCILHSSEDNKKGTNFSRPIKASKM
Sbjct:    1 MTKKKSLQNFIKKASDVNLNTAIAMSKSLKDLEKQNRKKAKELYLTPFHLMKDPIEVKKVLDLRLSNIILQSFQINLIDIVPRHTCKLWDKASLYCDIKPGDLSYDNINLSELMDYYVSALIPYIIPSTYREGDKMLNVSQIVGRERSIIYSNKNESSYQSPHKEVTNDTFNVIEIDDSIQLSSKSPEIANVTESEIINEDLNHFSPTNDWENSNLFPENISATPENDLSQCRFLEMFNKDKFSDFIVFLKSETVLYLHKFVLAAWNCNVEYLQDSQQFILNIDDEIVIKLLKHLYSDGKFNIDDIIDSDDCLALCKIFGLNRSLLQKHSLPVPFEPLCKSINTNQIISSNEKELKSLSAIKLPENNCDFSIDNILSKSIINLAQSTPNRFTLTNKTSEVSIKRSSNYSEKDLFESYDIDFDETTNLVVNNTLEAISNPSVQKIEEPLNNYSLHKKLCSFTNEPYLTNYPDTLTKLSHNNIDQPPDKRNNMEDVLTALPAQPEKNHIIQLSDRNPKIIEENFLSPTFCKMPNNNRISNNEIMKVTEPDLIIPSRVGDSINSEMCEENSDRLFDEIYYQSPISMTFGEEINFNHRNETEITKNNYLQHNSILTVKDNIILEDSPLPLRQRLGLKSLNSDINAVINISSDEELTNHEVKKPNKRSRDKIETIQSNSRFSTENGQEIKPKRSKTKSITDKLQRKPKKTLSEINIENVPKEPKVKKDKKSRKRKSSETLNGFITPIKQQFLADPSFTPVPDFSAMSTSVIKGKCMNFGIKPNLPKNYKIKILEKVHIYERIKNHSLSSSSNSDSTNVSFKENEESESNDSKDENVADNLNDNIQLYNFLKATYFRDILTYTPLDIKKVLAELKENNIKTSLKKLNDYFDKQCILHSSEDNKKGTNFSRPIKASKM 911
The following BLAST results are available for this feature:
BLAST of SMED30002307 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 4
Match NameE-valueIdentityDescription
A0A4S2LZE23.621e-826.57Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4S2LY274.009e-826.57Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A4S2LZ015.285e-826.57Uncharacterized protein OS=Opisthorchis felineus O... [more]
A0A5K4EQD83.607e-624.58Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
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BLAST of SMED30002307 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002307 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000036134.10.000e+099.56SMESG000036134.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002307 ID=SMED30002307|Name=SMED30002307|organism=Schmidtea mediterranea sexual|type=transcript|length=3640bp
ATTAGTAATTTTGTTTGGTAACTAAACAAGATTCAAAATCATTCTGTATA
TTGTTTCTGTATAAAAAAAGCTTATCGATATAATGACTAAAAAGAAATCG
TTACAAAATTTTATAAAAAAAGCGTCAGATGTTAATCTGAATACTGCAAT
AGCAATGTCAAAATCATTAAAAGATTTAGAAAAACAGAATAGAAAGAAAG
CTAAAGAATTATATTTAACTCCGTTTCATTTAATGAAAGATCCAATAGAA
GTTAAAAAAGTTCTAGATTTACGCCTTAGTAACATAATATTACAATCTTT
TCAAATAAATTTAATAGATATTGTACCACGCCATACATGTAAATTATGGG
AAAAAGCTTCTTTGTATTGTGACATAAAGCCAGGAGATTTGAGTTATGAC
AATATTAATTTATCTGAATTAATGGACTATTACGTATCAGCTCTAATTCC
TTATATAATTCCATCAACCTATCGAGAAGGAGATAAAATGCTTAATGTAT
CACAAATTGTCGGACGAGAAAGGTCAATTATTTATTCGAATAAAAATGAA
TCGAGTTACCAATCTCCACATAAAGAAGTCACCAACGATACTTTCAATGT
CATTGAAATTGACGATTCAATCCAGTTATCATCAAAATCCCCAGAAATTG
CAAATGTAACGGAGAGCGAAATTATAAATGAAGATTTAAATCATTTCTCA
CCTACAAATGATTGGGAAAATTCAAATTTATTCCCGGAAAATATTTCCGC
TACACCTGAAAATGATTTAAGCCAATGTCGATTTCTGGAAATGTTTAACA
AAGATAAATTTTCCGATTTTATAGTATTTTTAAAATCAGAAACTGTTTTG
TATTTGCACAAATTTGTATTGGCAGCTTGGAATTGCAATGCTGAATATTT
ACAGGATTCTCAGCAATTCATATTGAATATAGACGATGAAATTGTAATCA
AACTATTAAAGCATCTCTATTCAGACGGCAAATTTAATATTGATGATATT
ATTGACAGTGATGATTGTTTGGCGCTTTGTAAAATATTTGGACTCAATCG
GTCACTGTTACAGAAACATAGTTTACCTGTTCCCTTTGAACCACTTTGTA
AAAGTATTAATACAAATCAAATAATATCAAGCAATGAAAAGGAATTAAAA
TCTTTAAGTGCAATAAAACTTCCTGAAAACAACTGTGATTTTTCAATAGA
TAATATCCTATCTAAATCCATAATAAATCTCGCACAATCAACTCCAAATC
GCTTTACTTTAACCAATAAAACCTCCGAAGTAAGTATTAAAAGGAGTTCA
AATTATTCCGAAAAAGATTTATTTGAATCGTACGATATAGACTTTGATGA
AACGACAAATTTGGTGGTAAATAACACACTTGAAGCAATCTCAAATCCAA
GCGTTCAGAAGATCGAAGAACCTCTAAATAATTACAGTTTGCATAAGAAA
TTATGTTCATTCACCAATGAGCCTTATCTTACTAATTATCCAGATACTCT
TACTAAACTTTCCTATAACAATATAGACCAACCTCCCGATAAAAGAAACA
ACATGGAAGATGTTCTCACAGCTTTGCCAGCACAACCAGAAAAAAATCAT
ATAATTCAATTATCAGATCGAAATCCTAAAATTATCGAGGAAAATTTTCT
CAGTCCTACATTTTGTAAAATGCCTAACAATAATAGAATTTCTAATAATG
AGATAATGAAGGTTACAGAACCTGACTTGATAATACCATCTCGGGTAGGT
GATTCCATTAATTCAGAAATGTGTGAAGAGAACTCTGATAGGTTATTCGA
TGAAATTTATTATCAATCACCAATCTCAATGACATTTGGCGAAGAAATAA
ATTTTAATCACAGAAATGAAACTGAGATTACGAAAAACAATTACCTTCAA
CACAATTCAATTTTAACAGTAAAAGATAACATTATCCTAGAAGATTCACC
TCTTCCACTTCGCCAGAGGCTTGGATTGAAATCTCTTAATAGCGATATCA
ATGCTGTTATCAATATATCAAGCGATGAAGAATTGACCAATCATGAAGTC
AAGAAACCCAACAAACGTAGCAGAGATAAAATTGAAACTATTCAATCAAA
TTCAAGATTTTCTACTGAAAACGGTCAGGAAATAAAACCAAAGAGATCCA
AAACAAAAAGTATAACTGATAAACTTCAACGAAAACCTAAGAAAACTCTA
AGTGAAATTAATATCGAAAATGTACCAAAAGAACCGAAAGTGAAGAAGGA
TAAGAAATCTAGAAAGCGAAAATCATCAGAAACATTAAATGGATTTATTA
CACCTATAAAGCAACAATTTTTAGCAGATCCGTCGTTCACACCAGTCCCG
GATTTTTCTGCCATGTCAACTTCAGTTATTAAGGGAAAATGTATGAATTT
CGGTATTAAACCTAATTTACCAAAAAATTATAAAATAAAAATTCTTGAGA
AAGTGCATATTTATGAGCGTATTAAAAATCACAGTTTAAGTTCCAGTTCA
AACTCTGACTCGACGAATGTATCTTTTAAGGAAAATGAAGAAAGCGAGTC
AAATGATTCAAAAGATGAAAATGTTGTGGATAATTTAAATGACAATATCC
AATTGTATAACTTTCTCAAAGCTACATATTTCAGAGATATATTAACATAC
ACACCACTCGACATCAAGAAAGTTTTAGCGGAATTGAAAGAAAATAACAT
AAAAACATCCCTGAAAAAACTAAATGACTATTTTGATAAACAATGCATCT
TGCATTCTAGTGAAGATAATAAAAAAGGCACCAATTTTAGCAGGCCGATT
AAAGCGTCAAAAATGATTTTGTGAATATATATATATATATATTATTTCAT
TAAAAGAAAAGTTTAATTTTTGAATAAAACTTCTTGTATATTAAAATATG
CTCGTAATAGGTAAATTGTGTCAATTGTATTGTCACTAAGGCTGGTTCTA
ATTTTTGTGCAACTTAACCAGCTGCAGAATAAGCTCTCTCTGCTTCCACA
CTCGTCGGCTTTAATGTCAAGAGATGGTTGAACGCTATCGTCAGATACTT
TTCACGCCTTTATTCTTCTTCAAGCAGTGCGATTTCCTTTTTTACTAGTT
TCGTTAAAATTACAATATTGGTTATTGGTGCGTTGGAAATTTTATTTATT
TTGATTTGATCGTGATTTTTAAAAGGCCAATTGTCACCTGTCTTTCATCG
TTAACGGATTTACCTCAGCGATTCCCTCTACACCATCAGATGAATGATTT
GACCAGTGACTTTTCAGGTAGCATGTAAACCACTATTTGAACCTCCAGCA
GTTTTTGCATTTGAGCATATCTTTTCCATTTCGTTTATAGTAGTCCCATA
CCGTCTTCACTGATTCATAGTCAAATATTTTTTTGGGAATAATGCCATTT
TGAAAAAAATACATTTATAATTTTGAAAATTATAATGATCGCAATCAATA
GATCATTGTAATGGTCTTCAATTATTATTTATAAATTAACAAGTATGGTG
CCCGAACAGCCAATCAAATCCGAGTAAGTGCAAATTGTGCAAAATCAATA
TTTACTGCAATATTATGTACACATGCTATAATTTATACATCACACATCAT
GTAACTATGACGATGCAAGTTGTAACATGTATGAGTATGA
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protein sequence of SMED30002307-orf-1

>SMED30002307-orf-1 ID=SMED30002307-orf-1|Name=SMED30002307-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=914bp
MTKKKSLQNFIKKASDVNLNTAIAMSKSLKDLEKQNRKKAKELYLTPFHL
MKDPIEVKKVLDLRLSNIILQSFQINLIDIVPRHTCKLWEKASLYCDIKP
GDLSYDNINLSELMDYYVSALIPYIIPSTYREGDKMLNVSQIVGRERSII
YSNKNESSYQSPHKEVTNDTFNVIEIDDSIQLSSKSPEIANVTESEIINE
DLNHFSPTNDWENSNLFPENISATPENDLSQCRFLEMFNKDKFSDFIVFL
KSETVLYLHKFVLAAWNCNAEYLQDSQQFILNIDDEIVIKLLKHLYSDGK
FNIDDIIDSDDCLALCKIFGLNRSLLQKHSLPVPFEPLCKSINTNQIISS
NEKELKSLSAIKLPENNCDFSIDNILSKSIINLAQSTPNRFTLTNKTSEV
SIKRSSNYSEKDLFESYDIDFDETTNLVVNNTLEAISNPSVQKIEEPLNN
YSLHKKLCSFTNEPYLTNYPDTLTKLSYNNIDQPPDKRNNMEDVLTALPA
QPEKNHIIQLSDRNPKIIEENFLSPTFCKMPNNNRISNNEIMKVTEPDLI
IPSRVGDSINSEMCEENSDRLFDEIYYQSPISMTFGEEINFNHRNETEIT
KNNYLQHNSILTVKDNIILEDSPLPLRQRLGLKSLNSDINAVINISSDEE
LTNHEVKKPNKRSRDKIETIQSNSRFSTENGQEIKPKRSKTKSITDKLQR
KPKKTLSEINIENVPKEPKVKKDKKSRKRKSSETLNGFITPIKQQFLADP
SFTPVPDFSAMSTSVIKGKCMNFGIKPNLPKNYKIKILEKVHIYERIKNH
SLSSSSNSDSTNVSFKENEESESNDSKDENVVDNLNDNIQLYNFLKATYF
RDILTYTPLDIKKVLAELKENNIKTSLKKLNDYFDKQCILHSSEDNKKGT
NFSRPIKASKMIL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
PLANA:0002111X2 cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 815..830
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 652..669
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 652..736
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 805..830
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 681..707
NoneNo IPR availablePANTHERPTHR21541BTB POZ DOMAIN CONTAINING 12coord: 655..900
coord: 5..265
NoneNo IPR availableCDDcd18303BTB_POZ_Rank-5coord: 236..298
e-value: 0.0012983
score: 37.6879