SMED30002305

Overview
NameSMED30002305
Smed IDSMED30002305
Length (bp)1591
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002305 (SMED30002305) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002305 (SMED30002305) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002305 (SMED30002305) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002305 (SMED30002305) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002305

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30002305h1SMcG0000745 Contig1320GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30002305h1SMcG0000745 Contig2310GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30002305h1SMcG0005068 Contig2310GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
gutSMED30002305h1SMcG0000745 dd_Smed_v4_7461_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30002305h1SMcG0000745 dd_Smed_v4_7461_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30002305h1SMcG0000745 dd_Smed_v4_7461_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
dorsal ventral margin of the whole animalSMED30002305h1SMcG0000745 dd_Smed_v4_7461_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of SMED30002305 vs. Planmine SMEST
Match: SMESG000047320.1 (SMESG000047320.1)

HSP 1 Score: 952.97 bits (2462), Expect = 0.000e+0
Identity = 464/467 (99.36%), Postives = 466/467 (99.79%), Query Frame = 3 
Query:   96 MIYSNFILLLTIYFCACEDCPPNVNDQFTFASNMSVSQAAIYHKYAEITCCVVGFTDIKWEFRRNKSQNWSYTIPGNYDLSDENRKISFSAVEFEDTGYYKCTAKNYDDDIIYGETLLVVGCSKPHTKMIITNRTLETNFHELGTGRLCCSLSTINWAEAIFVFWIYKNSNSTIRTWQFNIMSEFITSKSGRISIYVDPKITKSPCEEYIKVDTCFEIERLQITDSGNCTCRFSSFDMVEQEFTLRVLSDVTNTILYIYCSVSIGSAIVLLFLLLWIFYYDLMIISTLIVGSKSKNFSIAVLIIYDDQDQLSFSTAVKISEILSRKGFSTYLFDRDKKFGCEFHSDYLSSMAESNQILFLCTEKFLENESMLFCLNNSTQYKNKRNFVFFNSRKALLQHFRNLKEINQIYQVIKFSTKYNVWKSNYDKDSFMRRRFISWIMSGFCYSTLKRNIKPKTIEGHVNDSYK 1496
            MIYSNFILLLTIYFCACEDCPPNVNDQFTFASNMSVSQAAIYHKYAEITCCVVGFTDIKWEFRRNKSQNWSYTIPGNYDLSDENRKISFSAVEFEDTGYYKCTAKNYDDDIIYGETLLVVGCSKP+TKMIITNRTLETNFHELGTGRLCCSLSTINWAEAIFVFWIYKNSNSTIRTWQFNIMSEFITSKSGRISIYV+PKITKSPCEEYIKVDTCFEIERLQ TDSGNCTCRFSSFDMVEQEFTLRVLSDVTNTILYIYCSVSIGSAIVLLFLLLWIFYYDLMIISTLIVGSKSKNFSIAVLIIYDDQDQLSFSTAVKISEILSRKGFSTYLFDRDKKFGCEFHSDYLSSMAESNQILFLCTEKFLENESMLFCLNNSTQYKNKRNFVFFNSRKALLQHFRNLKEINQIYQVIKFSTKYNVWKSNYDKDSFMRRRFISWIMSGFCYSTLKRNIKPKTIEGHVNDSYK
Sbjct:    1 MIYSNFILLLTIYFCACEDCPPNVNDQFTFASNMSVSQAAIYHKYAEITCCVVGFTDIKWEFRRNKSQNWSYTIPGNYDLSDENRKISFSAVEFEDTGYYKCTAKNYDDDIIYGETLLVVGCSKPNTKMIITNRTLETNFHELGTGRLCCSLSTINWAEAIFVFWIYKNSNSTIRTWQFNIMSEFITSKSGRISIYVNPKITKSPCEEYIKVDTCFEIERLQKTDSGNCTCRFSSFDMVEQEFTLRVLSDVTNTILYIYCSVSIGSAIVLLFLLLWIFYYDLMIISTLIVGSKSKNFSIAVLIIYDDQDQLSFSTAVKISEILSRKGFSTYLFDRDKKFGCEFHSDYLSSMAESNQILFLCTEKFLENESMLFCLNNSTQYKNKRNFVFFNSRKALLQHFRNLKEINQIYQVIKFSTKYNVWKSNYDKDSFMRRRFISWIMSGFCYSTLKRNIKPKTIEGHVNDSYK 467
[Close]
BLAST of SMED30002305 vs. Planmine SMEST
Match: SMESG000047314.1 (SMESG000047314.1)

HSP 1 Score: 90.1225 bits (222), Expect = 5.001e-19
Identity = 96/467 (20.56%), Postives = 207/467 (44.33%), Query Frame = 3 
Query:  114 ILLLTIYFCACEDCPPNVNDQF--TFASNMSVSQAAIYHKYAEITCCVVGFTDIKWEFRRNKSQNWSYTIPGNYDLSDENRKISFSAV-EFEDTGYYKCTAKN-YDDDIIYGETLLVVGCSKPHTKMIITNRTLETNFHELGTG-RLCCSLSTINWAEAIFVFWIYKNSNSTIRTWQFNIMSEFITSKSGRISIYVDPKITKSPCEEYIKVDTCFEIERLQITDSGNCTCRFSSFDMVEQEFTLRVLSDVTNTILYIYCSVSIGSAIVLLFLL-----LWIFYYDLMIIST----LIVGSKSKNFSIAVLIIYDDQDQLSFSTAVKISEILSRKGFSTYLFDRDKKFGCEFHSDYLSSMAESNQILFLCTEKFLENESMLFCLNNSTQYKN--KRNFVFFNSRKALLQHFRNLKEINQIYQVIKFSTKYNVWKSNYDKDSFMRRRF---ISWIMSGFCYSTLKRNIK 1457
            + +L I F    +  PN +     T       + + I  KY  + CC  GFT I W+FR+ ++  W  +    +   + NR +      +  DTGYY+CTA +  ++ + +   +  +  ++     ++ ++++  + +++G      C   +    +A+ + W++   NS     + + +S  I +         +  +  +P  + ++V + F I  LQ+ D+G   C+        ++    ++ DV      I  ++ +   +V L LL     +W + +D++++      +   + +K + ++  IIY D D  S   A KI   +  +    +LFDRDK++G E   D    +  S+ +L L +   ++N+++L  L +S Q     +R +V+  S+KA+++  R      + Y   + + +       +D +S    +F    SWI+S      +++  +
Sbjct:   11 LFMLVIDFGVSANPCPNQSRSLNPTLLIEPPFTMSGIEGKYFCMECCFDGFTKILWDFRKLRTTVWD-SFYDAWRFENSNRTLCIKKPNQLYDTGYYRCTATDALNNGLNHSIFVYFLLINENRNLTLVPDKSIPKSDYKIGNDLSFSCEAKSFKPNKAVSIQWLHSKLNSYKNISRSHFISNIIQTG-------FNETLESNP--DAVRVFSNFSISHLQLVDNGTYKCKIEQDGTTVEQIYYALIIDVQ-----IKWTLLLAILVVFLVLLSSAAGIWHYRFDILVVYKTYFDMFPNNNTKEYDLS--IIYQDDDFSSAQVAFKIVSFMECQNLHCWLFDRDKQYGQEILIDLSKILTHSHTVLILVSLSSIQNDNLLSNL-SSIQLNPSVRRLYVWNPSKKAIVKTVRRFDSSAEKYLTRELNLQSFCHGFGFDWNSAENIKFYPLTSWIISSLPSLVIRKKFQ 459
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BLAST of SMED30002305 vs. Planmine SMEST
Match: SMESG000047314.1 (SMESG000047314.1)

HSP 1 Score: 89.3521 bits (220), Expect = 8.544e-19
Identity = 94/454 (20.70%), Postives = 201/454 (44.27%), Query Frame = 3 
Query:  120 LLTIYFCACEDCPPNVNDQF--TFASNMSVSQAAIYHKYAEITCCVVGFTDIKWEFRRNKSQNWSYTIPGNYDLSDENRKISFSAV-EFEDTGYYKCTAKN-YDDDIIYGETLLVVGCSKPHTKMIITNRTLETNFHELGTG-RLCCSLSTINWAEAIFVFWIYKNSNSTIRTWQFNIMSEFITSKSGRISIYVDPKITKSPCEEYIKVDTCFEIERLQITDSGNCTCRFSSFDMVEQEFTLRVLSDVTNTILYIYCSVSIGSAIVLLFLL-----LWIFYYDLMIIST----LIVGSKSKNFSIAVLIIYDDQDQLSFSTAVKISEILSRKGFSTYLFDRDKKFGCEFHSDYLSSMAESNQILFLCTEKFLENESMLFCLNN-STQYKNKRNFVFFNSRKALLQHFRNLKEINQIYQVIKFSTKYNVWKSNYDKDSFMRRRF---ISWIMSGF 1427
            +L I F    +  PN +     T       + + I  KY  + CC  GFT I W+FR+ ++  W  +    +   + NR +      +  DTGYY+CTA +  ++ + +   +  +  ++     ++ ++++  + +++G      C   +    +A+ + W++   NS     + + +S  I +         +  +  +P  + ++V + F I  LQ+ D+G   C+        ++    ++ DV      I  ++ +   +V L LL     +W + +D++++      +   + +K + ++  IIY D D  S   A KI   +  +    +LFDRDK++G E   D    +  S+ +L L +   ++N+++L  L++       +R +V+  S+KA+++  R      + Y   + + +       +D +S    +F    SWI+S  
Sbjct:    1 MLVIDFGVSANPCPNQSRSLNPTLLIEPPFTMSGIEGKYFCMECCFDGFTKILWDFRKLRTTVWD-SFYDAWRFENSNRTLCIKKPNQLYDTGYYRCTATDALNNGLNHSIFVYFLLINENRNLTLVPDKSIPKSDYKIGNDLSFSCEAKSFKPNKAVSIQWLHSKLNSYKNISRSHFISNIIQTG-------FNETLESNP--DAVRVFSNFSISHLQLVDNGTYKCKIEQDGTTVEQIYYALIIDVQ-----IKWTLLLAILVVFLVLLSSAAGIWHYRFDILVVYKTYFDMFPNNNTKEYDLS--IIYQDDDFSSAQVAFKIVSFMECQNLHCWLFDRDKQYGQEILIDLSKILTHSHTVLILVSLSSIQNDNLLSNLSSIQLNPSVRRLYVWNPSKKAIVKTVRRFDSSAEKYLTRELNLQSFCHGFGFDWNSAENIKFYPLTSWIISSL 437
[Close]
BLAST of SMED30002305 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.219e-14
Identity = 90/391 (23.02%), Postives = 162/391 (41.43%), Query Frame = 3 
Query:  114 ILLLTIYFCACEDCPPNVNDQFT-----FASNMSVSQAAIYHKYAEITCCVVGFTDIKWEFRRNKSQNWSYTIPG-NYDLSDENRKISFSAVEFEDTGYYKCTAKNYDDDIIYGETLLVVGCSKPHTKMIITNRT--LETNFHELGTGRLCCSLSTINWAEAIFVFWIYKNSNSTIRTWQFNIMSEFITSKSGRISIYVDPKITKSPCEEYIKVDT----CFE--IERLQITDSGNCTCRFSS-FDMVEQEFTLRVLSDVTNTIL---YIYCSVSIGSAIVLLFLLLWIFYYDLMII---STLIVGSKSKNF-SIAVLIIYDDQDQLSFSTAVKISEILSRKGFSTYLFDRDKKFGCEFHSDYLSSMAESNQILFLCTEKFLENESMLFCL 1220
            +L+LT+   +   CP   ++Q       F  +M  S          + CC+ G ++I W F+ + S++++  I G +Y L + NRK+    V     G YKC   N   + +  +T ++V  S  +  + I + +   E  F         C +     +   F +   KN   T      +I++    +K       V+     S  +E  K        FE  I  +Q +  G  +C     F+  E+ F + V       +L   +IY +  I    +L+F+L + F YD  I+   S   +  K KNF S     +YD  D+ +F T  K+ + +  +  + Y + RD+ +G     +    +  S+ I+F  ++K  +N    FC+
Sbjct:   14 VLVLTLGLESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRT------SILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSCLVEDRFEKDERVFNVYV------KLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCI 392
[Close]
BLAST of SMED30002305 vs. Planmine SMEST
Match: SMESG000016701.1 (SMESG000016701.1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.235e-14
Identity = 90/391 (23.02%), Postives = 161/391 (41.18%), Query Frame = 3 
Query:  114 ILLLTIYFCACEDCPPNVNDQFT-----FASNMSVSQAAIYHKYAEITCCVVGFTDIKWEFRRNKSQNWSYTIPG-NYDLSDENRKISFSAVEFEDTGYYKCTAKNYDDDIIYGETLLVVGCSKPHTKMIITNRT--LETNFHELGTGRLCCSLSTINWAEAIFVFWIYKNSNSTIRTWQFNIMSEFITSKSGRISIYVDPKITKSPCEEYIKVDT----CFE--IERLQITDSGNCTCRFSSFDMVEQEFTL--RVLSDVTNTI--LYIYCSVSIGSAIVLLFLLLWIFYYDLMII---STLIVGSKSKNF-SIAVLIIYDDQDQLSFSTAVKISEILSRKGFSTYLFDRDKKFGCEFHSDYLSSMAESNQILFLCTEKFLENESMLFCL 1220
            +L+LT+   +   CP   ++Q       F  +M  S          + CC+ G ++I W F+ + S++++  I G +Y L + NRK+    V     G YKC   N   + +  +T ++V  S  +  + I + +   E  F         C +     +   F +   KN   T      +I++    +K       V+     S  +E  K        FE  I  +Q +  G  +C      +VE  F    RV +     +   +IY +  I    +L+F+L + F YD  I+   S   +  K KNF S     +YD  D+ +F T  K+ + +  +  + Y + RD+ +G     +    +  S+ I+F  ++K  +N    FC+
Sbjct:   36 VLVLTLGLESDGKCPEPRSEQLNQNQPFFTRDMYESSVMSVSMIGYVHCCIYGVSNINWWFKNSDSKHFTQIIKGKDYQLENNNRKLIVLNVSKNSKGIYKCVGNNEKGNSLSNQTFVIVVQSTYNKDIEIVDASDITELTFVPYSNYTFYCLVKVFPGSICNFSWNRKKNGKRT------SILAHSNLTKKRTYDTLVEGYSGYSQLKELNKQGNFRNFLFELIIFNIQTSHEGEYSC------LVEDRFEKDERVFNVYVKLLGKEHIYIASGIVGCFILIFILAYEFQYDCKILLKKSLQFLTIKEKNFGSYNFFFLYDFDDEYTFQTIAKVVKKIESRYCTVYFYHRDQIYGKIMFEELYEKLQRSDVIVFWVSQKIWKNPISSFCI 414
The following BLAST results are available for this feature:
BLAST of SMED30002305 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002305 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000047320.10.000e+099.36SMESG000047320.1[more]
SMESG000047314.15.001e-1920.56SMESG000047314.1[more]
SMESG000047314.18.544e-1920.70SMESG000047314.1[more]
SMESG000016701.12.219e-1423.02SMESG000016701.1[more]
SMESG000016701.12.235e-1423.02SMESG000016701.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002305 ID=SMED30002305|Name=SMED30002305|organism=Schmidtea mediterranea sexual|type=transcript|length=1591bp
GATAATTTGCGGTAAATCCAATTCAAATGGAATATTTTACATTTTGATAT
GTAACTGAATTTATTTGATTATTTTTGATAAATTAAATTTGTTTAATGAT
TTATTCAAATTTTATTTTATTACTGACAATTTATTTTTGTGCCTGTGAAG
ATTGCCCACCGAATGTTAATGATCAGTTTACTTTCGCTTCTAATATGAGT
GTAAGCCAAGCAGCAATTTATCATAAGTATGCAGAAATTACTTGTTGTGT
TGTGGGATTTACAGATATAAAATGGGAGTTTCGCAGAAATAAATCTCAGA
ATTGGTCATATACAATACCTGGAAATTATGATTTAAGTGATGAAAACAGG
AAAATCTCATTTTCCGCAGTAGAATTCGAAGATACAGGATATTACAAGTG
TACTGCAAAAAACTATGATGATGATATAATCTACGGAGAAACGCTCTTGG
TTGTCGGATGCTCAAAACCCCATACAAAAATGATTATAACCAACAGAACT
TTGGAAACGAATTTTCACGAACTTGGCACCGGCAGGCTGTGCTGTTCTCT
CTCCACTATAAACTGGGCTGAGGCGATTTTTGTATTTTGGATTTATAAAA
ATTCAAATTCAACCATTAGAACATGGCAATTTAACATCATGAGCGAATTT
ATAACTAGTAAATCAGGCCGTATAAGTATTTATGTGGATCCTAAAATTAC
CAAATCACCATGTGAAGAATATATCAAAGTGGACACCTGCTTTGAAATTG
AACGTCTTCAAATAACTGATTCTGGGAATTGTACCTGCCGATTTTCCAGC
TTTGACATGGTAGAACAAGAATTTACTCTGCGAGTTTTATCAGATGTAAC
TAACACGATTTTATATATTTATTGTTCTGTGAGCATCGGTTCAGCAATAG
TTCTACTTTTTTTGTTATTATGGATATTTTATTATGATTTGATGATCATC
AGCACTCTTATTGTCGGTTCAAAATCCAAAAATTTCAGTATCGCAGTCCT
TATTATCTATGATGACCAAGACCAATTAAGTTTTTCGACTGCAGTAAAAA
TTAGTGAAATTCTCTCTAGAAAAGGATTTTCTACGTATCTCTTTGATCGG
GACAAAAAATTTGGATGTGAATTTCATAGCGATTATTTATCGAGCATGGC
TGAATCGAATCAAATTCTTTTTCTTTGCACGGAAAAATTCCTGGAAAATG
AATCAATGTTATTTTGTTTAAATAATTCGACTCAATATAAAAACAAAAGG
AATTTTGTGTTTTTTAATTCGAGAAAAGCACTTCTACAGCATTTTCGAAA
TCTAAAGGAAATAAATCAAATCTATCAAGTGATCAAGTTTTCTACCAAAT
ATAATGTTTGGAAATCTAATTATGACAAAGATTCGTTTATGAGGAGAAGA
TTTATAAGTTGGATTATGTCTGGGTTCTGTTACTCTACATTGAAACGAAA
TATTAAACCCAAAACAATTGAGGGTCATGTTAATGATTCTTACAAAATAG
ATATTTAAAACTTTTCCATTTCATCCATTTTTAACTCCACTGTTTCCTTG
TACAATTACAGAAAACTAATTGTTAAAATCTTATATATGTT
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protein sequence of SMED30002305-orf-1

>SMED30002305-orf-1 ID=SMED30002305-orf-1|Name=SMED30002305-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=471bp
MIYSNFILLLTIYFCACEDCPPNVNDQFTFASNMSVSQAAIYHKYAEITC
CVVGFTDIKWEFRRNKSQNWSYTIPGNYDLSDENRKISFSAVEFEDTGYY
KCTAKNYDDDIIYGETLLVVGCSKPHTKMIITNRTLETNFHELGTGRLCC
SLSTINWAEAIFVFWIYKNSNSTIRTWQFNIMSEFITSKSGRISIYVDPK
ITKSPCEEYIKVDTCFEIERLQITDSGNCTCRFSSFDMVEQEFTLRVLSD
VTNTILYIYCSVSIGSAIVLLFLLLWIFYYDLMIISTLIVGSKSKNFSIA
VLIIYDDQDQLSFSTAVKISEILSRKGFSTYLFDRDKKFGCEFHSDYLSS
MAESNQILFLCTEKFLENESMLFCLNNSTQYKNKRNFVFFNSRKALLQHF
RNLKEINQIYQVIKFSTKYNVWKSNYDKDSFMRRRFISWIMSGFCYSTLK
RNIKPKTIEGHVNDSYKIDI*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000043dorsal ventral margin of the whole animal
PLANA:0000070intestinal phagocyte
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR003599Ig_sub
IPR035897Toll_tir_struct_dom_sf
IPR036179Ig-like_dom_sf
IPR013783Ig-like_fold
IPR000157TIR_dom
IPR007110Ig-like_dom
Vocabulary: biological process
TermDefinition
GO:0007165signal transduction
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003599Immunoglobulin subtypeSMARTSM00409IG_3ccoord: 135..248
e-value: 18.0
score: 9.3
coord: 35..121
e-value: 2.7
score: 16.4
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 27..112
e-value: 9.2E-6
score: 27.8
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilyGENE3DG3DSA:3.40.50.10140coord: 300..435
e-value: 6.1E-6
score: 28.2
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilySUPERFAMILYSSF52200Toll/Interleukin receptor TIR domaincoord: 301..390
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 22..106
score: 8.067
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..17
score: 0.81
NoneNo IPR availableTMHMMTMhelixcoord: 255..277
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 38..120