SMED30002282

Overview
NameSMED30002282
Smed IDSMED30002282
Length (bp)1956
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002282 (SMED30002282) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002282 (SMED30002282) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30002282

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parapharyngeal regionSMED30002282h1SMcG0015055 SmedASXL_064638SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30002282h1SMcG0015055 SmedASXL_007827SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30002282 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.002457:37019..39250 -9732
Homology
BLAST of SMED30002282 vs. TrEMBL
Match: A0A1S3JYU6 (C5a anaphylatoxin chemotactic receptor 1 OS=Lingula unguis OX=7574 GN=LOC106177380 PE=3 SV=1)

HSP 1 Score: 122.479 bits (306), Expect = 1.351e-26
Identity = 87/293 (29.69%), Postives = 158/293 (53.92%), Query Frame = -3
Query:  149 FIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCR-GKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRMRERLKTMREDTIGIXXXXXXXXXXXXXXXXXLFVG--ILFLVFFTPSISIWTTMSLFTFYNVDINPN---LMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELINLI 1009
             +++  KS+   S ++Y R LAI D+  L+ G+ PV+I  ++  ++T ++ W+C++  +  +  GD A W+++ V+ +RF++V +P K K W TK +A++        SC+   + FV+L +K        G Q  +    C   +KE+  +E V+R  I  I + F+  L+I + N++I+  +  + +  R++  G SSS +  + +       + +   + F+V  TPSI    T+ L + Y V + P+   L   L A+TD L Y  +S NFF+Y ++G+ FR+E   LI
Sbjct:   52 LLVMRGKSLRPSSASIYLRYLAIADTSTLVLGVFPVWIRALTTVDVTALHPWTCKLHVYFLYSAGDCAVWVIVAVTVDRFLSVTFPFKAKLWCTKRRALI--------SCV--VITFVALLVKNSHLLWTRGFQYDATGAVCGVARKEFAFFETVIRSWIGFILYAFIPILSIIILNSLIAFGL-HKAQHQRQNLFGSSSSQAVTETANFRSSTYMLLALSLAFVVLVTPSI----TVLLLSPYWV-VTPHDRALEELLQAITDSLVYTNHSINFFLYCLTGKGFRREFFLLI 328          
BLAST of SMED30002282 vs. TrEMBL
Match: A0A0K0PVM1 (Orphan G-protein coupled receptor 1 OS=Platynereis dumerilii OX=6359 PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.132e-21
Identity = 79/291 (27.15%), Postives = 141/291 (48.45%), Query Frame = -3
Query:  143 IILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCR-GKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRM--RERLKTMREDTIGIXXXXXXXXXXXXXXXXXLFVGILFLVFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELINLIHC 1006
            I++SR  +   + A+Y  VLAI D+ AL +G+IP ++   +      ++  +CR ++ + +   D A WIL+  + +RFIAV +P +   W T  KAI+ +  L + + +KN        + V  ++    +++ + IK C   + EY  +E  VRP +  +    +  L I   N  I  ++    R+K + +             +      M + V  LFL+  TPS+ ++   S +   + D          A+T++L YL +S NFF+Y +SG+RFR+E +    C
Sbjct:   61 IVMSRNKMRGNTTAIYLPVLAIADAFALFSGIIPEWMEACNIIKFKELSPGTCRFEKIIFYTSADTAIWILVAFTCDRFIAVCFPFRKSRWCTSRKAIIISASLLVAAFIKN--------MHVFWTRGAQYDENGTFIKNCGYPRPEYKNFERNVRPWLVFVLVNLIPFLVILSCNLAIVYKLLAATRIKDISKPN----------SKYSQTTLMCVGVSFLFLLTTTPSVVMYIGRSEWDKSHTD----AYEVAKAVTNVLVYLNHSLNFFVYCMSGKRFRREFLMFCCC 329          
BLAST of SMED30002282 vs. TrEMBL
Match: A0A0K0PUJ9 (Orphan G-protein coupled receptor 49 OS=Platynereis dumerilii OX=6359 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.077e-19
Identity = 76/289 (26.30%), Postives = 143/289 (49.48%), Query Frame = -3
Query:  143 FIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRMRERLKTMREDTIGIXXXXXXXXXXXXXXXXXLFVGILFLVFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELINLIHC 1009
             I++ R+     +  +Y R++A+ D L LI G++P ++          ++  +C++++F+ +  GDV+ WIL+  + +RFIAV +P    ++    +A  + F++ + +  KN   F +     +    E  N S   +  C   + +  +E ++RP I       L  L I + N +I   +R  +++ R  T G    ++  K    +  M L     FLVF TPSI +        +++ D +  ++    A+ + L+Y+ +S NFF+Y +SG RFRKEL+  + C
Sbjct:   63 LIVMMRRKFQGTTTCIYLRLMALADLLVLIIGMVPEWLSANDIVIFKEVHPVTCKLEKFLQYTTGDVSIWILMCFTVDRFIAVCFPFSKTNFCKPGRARFYGFIVLLAAIFKNLCVFWT--RGPVYHSSEGDNSSLVLVSNCGRPQPFTYFEKLIRPWIVFALISVLPFLVIVICNVMI---IRALIES-RVVTSGNKIQSNMEKTYMQMTLMCLSASFAFLVFITPSIVLLIGKP---YWDNDSDSYIIA--KAVNNQLTYVNHSINFFLYCLSGARFRKELVRTLIC 340          
BLAST of SMED30002282 vs. TrEMBL
Match: R7U5J6 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_198894 PE=4 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 3.486e-18
Identity = 71/292 (24.32%), Postives = 140/292 (47.95%), Query Frame = -3
Query:  161 FIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRP-----MISLIGFYFLTALAIFVTNAIISCRMRE----RLKTMREDTIGIXXXXXXXXXXXXXXXXXLFVGILFLVFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKEL 1009
            F+ L+R+ +      +YFR L I DS AL+ GL+P++   ++ +++T  +   C+++ F+   F D + W++   + +R +AVV+PLK KS+ T+  A++   +++  + LKN    ++  ++      E  N S    K    + ++  YE  +RP      ++ I F F+TA  I + + +    + +    R++++   +  IS ++               V   F+V  +PSI I      +T    + +        +  ++  Y   + NF +Y+ SGR FR+EL
Sbjct:   39 FVTLARQILRHRVSTIYFRALCIADSGALLFGLLPIWPLYLAGYDVTAQHAVICKLRNFLFITFSDASIWLICAFTLDRLVAVVFPLKRKSYCTRRHALIAITVIWAVATLKNVPMLITWGIR------ETHNYSHHVSKCGVPEPDHAFYEEYIRPWLVFTTVTFIPFIFITACNIIIVHHVQRMGLSQATIARVRSITRQSSEISVASMSRMCLA--------VSFTFVVLVSPSIVILVGKPYWT--KTEQSEAKFHLARSFGNLCQYTNNAINFLLYLSSGREFRREL 314          
BLAST of SMED30002282 vs. TrEMBL
Match: R7UUD3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_169933 PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.279e-17
Identity = 83/293 (28.33%), Postives = 145/293 (49.49%), Query Frame = -3
Query:  149 FIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRMRERLKTMREDTIGIXXXXXXXXXXXXXXXXXLFVGILFLVFFTPSI------SIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELINLI 1009
             +++++K +   + +VY R++A  D   LITG+IP ++ Q        I+  +C++++F  +   D A WIL++ + +RFIAV +PL+ K +   S++  F     + +  KNF  F +   + IT    V N +  +    R   E+  +E  VRP I+      L    I V N  I  R    +K +R +   +S+S+ Q+ Q   +   + F    FLV  TPSI        WT  + +     DI+         +++ L Y+ +S NFF+Y ++G+RFR  L++L+
Sbjct:   63 LLVMAKKRMRGTTTSVYLRLMAFADLCVLITGMIPEWLEQADILVFKYIHPATCKLEKFTFYTSADTAIWILVIFTVDRFIAVCFPLEKKDYCLPSRSKYFATGALLLAVTKNFHVFWTRGAEYIT----VDNSTVLKSNCGRPTPEFKYFEFYVRPWIAFTTVNALPFCVILVCNIFI-IRALIAVKKLRSEQAIVSNSDRQLFQMSLMCLSASFS---FLVCITPSIILLIGRPHWTGSTAY-----DIS-------KGISNQLVYVNHSINFFLYCVTGKRFRVVLVHLL 335          
BLAST of SMED30002282 vs. Planmine SMEST
Match: SMESG000011554.1 (SMESG000011554.1)

HSP 1 Score: 549.28 bits (1414), Expect = 0.000e+0
Identity = 315/330 (95.45%), Postives = 320/330 (96.97%), Query Frame = -3
Query:  140 DGQFYFFTL*LKMLNDISNFINKYXXXXXXXXXXXXXXXSFIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRMRERLKTMREDTIGIXXXXXXXXXXXXXXXXXLFVGILFLVFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELINLIHCR 1129
            +G    F + L+++    NFINKYCLSLLLIIGCLGNGLSFIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRMRERLKTMREDTIGISSSNSQVKQSKSLLKMSLFVGILFLVFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELINLIHCR
Sbjct:    7 NGILLIFKVHLEIIRLTDNFINKYCLSLLLIIGCLGNGLSFIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRMRERLKTMREDTIGISSSNSQVKQSKSLLKMSLFVGILFLVFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELINLIHCR 336          

HSP 2 Score: 50.447 bits (119), Expect = 2.614e-6
Identity = 25/28 (89.29%), Postives = 27/28 (96.43%), Query Frame = -2
Query: 1269 MRLIINNGILLIFKVHLEIIRLTESVIN 1352
            MRLIINNGILLIFKVHLEIIRLT++ IN
Sbjct:    1 MRLIINNGILLIFKVHLEIIRLTDNFIN 28          
BLAST of SMED30002282 vs. Planmine SMEST
Match: SMESG000011555.1 (SMESG000011555.1)

HSP 1 Score: 317.39 bits (812), Expect = 2.250e-103
Identity = 182/323 (56.35%), Postives = 242/323 (74.92%), Query Frame = -3
Query:  140 MLNDISNFINKYXXXXXXXXXXXXXXXSFIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKV--ITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRMRERLKTMR---EDTIGIXXXXXXXXXXXXXXXXXLFVGILFLVFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELINLIHCR 1093
            M + IS  INK+ L ++L +G +GN LSFI+  R SIS  SL++YFRVLA  DSL LITGL+P F+YQI  F +TTI+DWSCRIQR++ F  GDVACW LILVS ER IAVV+PLK + W TKS+A+  +  LFIP+ +KNFM F++LELK   I   D   N + +EI+ C  K +YF YEN+VRP +SL  +YF+TA  IF+TN +IS  M+ RLK+MR   ++ +   S  SQ  QSKSLLKMSLF+GIL+LVF TPS++IWTT+++FT+Y V+I+  ++ FL+++TDIL+YLQYSTNFF+YVISG+RFR E+ NL+  +
Sbjct:    1 MFSQISGTINKFILPIILAVGLVGNILSFIVFCRNSISKSSLSIYFRVLATFDSLTLITGLVPGFLYQICSFKITTISDWSCRIQRYLVFSSGDVACWTLILVSLERMIAVVFPLKAREWCTKSRALTLSLFLFIPASIKNFMTFLTLELKTFFIVGAD---NNTLTEIRQCISKTKYFYYENLVRPFLSLTLYYFVTAAIIFITNGVISLAMKSRLKSMRYITQEAVKADSGKSQKTQSKSLLKMSLFIGILYLVFLTPSMTIWTTINMFTYYQVEISGEMIDFLFSVTDILAYLQYSTNFFVYVISGKRFRLEIKNLLRIK 320          
BLAST of SMED30002282 vs. Planmine SMEST
Match: SMESG000079764.1 (SMESG000079764.1)

HSP 1 Score: 135.961 bits (341), Expect = 5.022e-35
Identity = 95/290 (32.76%), Postives = 155/290 (53.45%), Query Frame = -3
Query:  158 FIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWS--CRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRMRERLKTMR----EDTIGIXXXXXXXXXXXXXXXXXLFVGILFLVFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELI 1009
            F I  RK+  + ++ +Y R+LA+ DS+ L+   +P  IY+    +M  I+  S  C IQR + F  GD++ W L  ++ +R +AVVYP +   W +    +    ++F  S LKNF  F +L              +S     C  +  + +YE+ VRP+ISLI +Y +  + + V   +I   MR+ +  +R     +   +S+   +   SKSL KMS+ + ILF V  TPSI+IWT MS    Y  DIN + +   Y +T+IL +  +S NF+++  +G RFR+E++
Sbjct:   30 FAIFLRKNFRTSTITIYIRILAVADSVLLLAAAVPN-IYRGFHGDMGEIDRTSVACVIQRLIMFIAGDISVWTLTAITIDRLVAVVYPHQNSFWKSNKNTLFVLIVIFALSLLKNFPVFFTL--------------ASINGTRCAARTSFEIYEDSVRPIISLILYYLIPTITMTVCTVMILKEMRKIVGKIRAMSNPNRNSVSADYMKNHYSKSLTKMSIAINILFFVLITPSITIWTLMS----YAKDINADAIALCYDITNILVFSHHSLNFYLFFFTGPRFRREVL 300          
BLAST of SMED30002282 vs. Planmine SMEST
Match: SMESG000079764.1 (SMESG000079764.1)

HSP 1 Score: 135.576 bits (340), Expect = 5.046e-35
Identity = 95/290 (32.76%), Postives = 155/290 (53.45%), Query Frame = -3
Query:  158 FIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWS--CRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRMRERLKTMR----EDTIGIXXXXXXXXXXXXXXXXXLFVGILFLVFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELI 1009
            F I  RK+  + ++ +Y R+LA+ DS+ L+   +P  IY+    +M  I+  S  C IQR + F  GD++ W L  ++ +R +AVVYP +   W +    +    ++F  S LKNF  F +L              +S     C  +  + +YE+ VRP+ISLI +Y +  + + V   +I   MR+ +  +R     +   +S+   +   SKSL KMS+ + ILF V  TPSI+IWT MS    Y  DIN + +   Y +T+IL +  +S NF+++  +G RFR+E++
Sbjct:   23 FAIFLRKNFRTSTITIYIRILAVADSVLLLAAAVPN-IYRGFHGDMGEIDRTSVACVIQRLIMFIAGDISVWTLTAITIDRLVAVVYPHQNSFWKSNKNTLFVLIVIFALSLLKNFPVFFTL--------------ASINGTRCAARTSFEIYEDSVRPIISLILYYLIPTITMTVCTVMILKEMRKIVGKIRAMSNPNRNSVSADYMKNHYSKSLTKMSIAINILFFVLITPSITIWTLMS----YAKDINADAIALCYDITNILVFSHHSLNFYLFFFTGPRFRREVL 293          
BLAST of SMED30002282 vs. Planmine SMEST
Match: SMESG000079761.1 (SMESG000079761.1)

HSP 1 Score: 134.035 bits (336), Expect = 2.805e-34
Identity = 84/294 (28.57%), Postives = 156/294 (53.06%), Query Frame = -3
Query:  152 FIILSRKSISSGSLAVYFRVLAICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWILILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLELKVITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRPMISLIGFYFLTALAIFVTNAIISCRMRERLKTM-------REDTIGIXXXXXXXXXXXXXXXXXLF-VGILFLVFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIYVISGRRFRKELINL 1009
            F I  R +  S S+  Y R+LA+ D++ +  G++P      +  ++   +D  C +QRF+ FF GD++ WILI ++ +R +AVV+P++  +W ++  +I+   ++F  S +KNF  F +L +        V N + +  +FC  ++ +  YE  +RP ISL+ +Y +  + + +   +I  +M    K +       R  ++ I++ N         L      + ILF++  +PSI IWT +S   ++   I    +  LY  T+IL +  +S NF+++ +SG RFRKE++ +
Sbjct:   30 FYIFLRPNFKSSSITKYMRILAVTDTILIFVGIVPNVYEGFNRKSLLIRSDAGCILQRFIMFFIGDISVWILIAITIDRLVAVVFPIQNTTWKSQKNSIVILIVVFSLSLIKNFPIFFTLSV--------VTNTNGT--RFCDTRERFAFYERKIRPFISLMMYYLIPTIIMTICTVVIILKMNRVAKKLDVMTNPKRRISMEINNVNFIRPHYSKSLTQMSIAINILFVLLLSPSIFIWTIIS---YWPASIQSEKISILYDSTNILVFSHHSLNFYLFFLSGPRFRKEVLQI 310          
The following BLAST results are available for this feature:
BLAST of SMED30002282 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1S3JYU61.351e-2629.69C5a anaphylatoxin chemotactic receptor 1 OS=Lingul... [more]
A0A0K0PVM11.132e-2127.15Orphan G-protein coupled receptor 1 OS=Platynereis... [more]
A0A0K0PUJ92.077e-1926.30Orphan G-protein coupled receptor 49 OS=Platynerei... [more]
R7U5J63.486e-1824.32G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
R7UUD31.279e-1728.33G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
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BLAST of SMED30002282 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002282 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000011554.12.614e-695.45SMESG000011554.1[more]
SMESG000011555.12.250e-10356.35SMESG000011555.1[more]
SMESG000079764.15.022e-3532.76SMESG000079764.1[more]
SMESG000079764.15.046e-3532.76SMESG000079764.1[more]
SMESG000079761.12.805e-3428.57SMESG000079761.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002282 ID=SMED30002282|Name=SMED30002282|organism=Schmidtea mediterranea sexual|type=transcript|length=1956bp
ATTATATTCATTCATTCCTCACCATTCTCAACATTAATTAAAAATGAATT
AGTTTATTATTCATGTACTGAATTAAAACAAAATTTGATTAAAATAAAAA
AAACAAAATGAAGTGCGAAAATATGTTTTCTTACTATTATCTGCAATGAA
TCAAGTTTATCAGTTCTTTTCTGAATCTTCTTCCACTGATGACATAAATG
AAAAAGTTTGTAGAATATTGCAAATAAGACAAGATGTCCGTCAACGCATA
GAGAAACCCCATTAAGTTAGGATTAATATCAACATTGTAAAACGTAAACA
AACTCATTGTTGTCCATATTGATATCGATGGAGTGAAGAACACCAAAAAA
AGTATTCCGACAAAAAGCGACATTTTCAATAAACTTTTCGACTGTTTTAC
TTGAGAATTACTCGAACTTATTCCAATCGTGTCTTCTCGCATCGTTTTCA
ACCGTTCTCTCATTCGGCAACTAATAATTGCGTTTGTCACAAAAATTGCT
AGAGCCGTTAGAAAATAAAACCCTATCAAACTAATCATCGGTCGAACAAC
ATTTTCATAAAGAAAATATTCTTTTTTCCCTCTGCAAAACTTTATTTCAC
TACTCGATTGATTTCCAACCTCATCTTTGCTTGTTATCACTTTCAACTCC
AAAGAAACAAACGCCATGAAGTTTTTCAAACAAGACGGAATGAATAGAAG
AAAAGTAAACAAAATCGCTTTGGATTTTGTGCTCCATGATTTGAGTTTCA
GAGGATATACGACGGCGATAAATCTTTCAAATGACACCAAAATCAAAATC
CAACAAGCTACATCGCCGAAAAAAAAGTTCACAAACCTCTGGATCCGACA
ACTCCAATCGTTGATTGTTGTCATGTTGAAATCAGAGATTTGGTAAATAA
AAACCGGAATCAAGCCGGTTATTAGCGCAAGACTGTCGCATATGGCCAAT
ACCCGAAAGTAAACCGCCAAGCTTCCACTGGAGATGCTTTTTCTCGAGAG
GATAATGAAGGACAGCCCATTACCGAGGCATCCTATAATGAGCAGCAGTG
ATAAACAATATTTGTTTATAAAATTGCTAATGTCATTTAGCATTTTTAAT
TAGAGAGTAAAAAAATAAAATTGCCCGTCTAATTAAAAGCTGAAATTTCG
TGCATTTTATCTTCCAATAGCATTGCGATTGGTAAATGCAGTTTCTCCTC
TTTTCTTTTGTGGGAATTTTATCATATTTCTTTTATCTCCGTTAAACGAC
TGAGATTTGATTAAAAATGTTTATTACTGACTCCGTCAGACGAATTATTT
CCAGATGCACTTTAAAAATCAATAAAATTCCGTTATTGATAATTAGCCTC
ATTCTTTACCCACCTTTTAGTTACCATTTAAAATACAGAGAACCCTCATG
GTAGCAATTTAGCTTAATAAGTAGCTAATCCTTGTTTTTTCAAAATGTTT
TCCAAATCAGACAGTGCTGAATTGGAGGAATAATAAGGTATTTGGCCTCT
GGCAACAGTATGCAGCTTCAAGGGGCTAATTAGCCGGGTATATAAGGGCT
CTAAAGAAAATTAATATCTTAATAAATTGTTATTATTTGTTGTCTTATTG
GTTCTTAAATTAAAATGTACTTATCGGAGAGTATACGGTCTTTGGATATG
AATCATAATTTTTAGTTTTTTTTATTTAATATTTTTGTATTTTCTCCGTT
TCTGTGAATCTTTTAAAGTTTTCTGTATCGATAAACAGGTTTGTTTTAAA
TTGGATAACTGTTAGTAAACTCGGAATTATCCTTATATTTATATCCCATT
TATTTTTGTAAATTTGCTTCATCCAAGTCGTGACAGGGTTATTGGATCTC
ATGACGGCTGTCACAACTCAGAGAATTAATAAATTAATCAAAAAGTACTA
AATTTTATTTTTACGAATTTTTTTATCAGTAAATTATTTATATATTATTT
ATTTAT
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protein sequence of SMED30002282-orf-1

>SMED30002282-orf-1 ID=SMED30002282-orf-1|Name=SMED30002282-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=319bp
MLNDISNFINKYCLSLLLIIGCLGNGLSFIILSRKSISSGSLAVYFRVLA
ICDSLALITGLIPVFIYQISDFNMTTINDWSCRIQRFVNFFFGDVACWIL
ILVSFERFIAVVYPLKLKSWSTKSKAILFTFLLFIPSCLKNFMAFVSLEL
KVITSKDEVGNQSSSEIKFCRGKKEYFLYENVVRPMISLIGFYFLTALAI
FVTNAIISCRMRERLKTMREDTIGISSSNSQVKQSKSLLKMSLFVGILFL
VFFTPSISIWTTMSLFTFYNVDINPNLMGFLYALTDILSYLQYSTNFFIY
VISGRRFRKELINLIHCR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 282..308
score: 25.32
coord: 9..33
score: 20.83
coord: 89..111
score: 20.24
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 24..300
e-value: 1.7E-19
score: 70.2
IPR000276G protein-coupled receptor, rhodopsin-likePROSITEPS00237G_PROTEIN_RECEP_F1_1coord: 95..111
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 3..318
e-value: 1.8E-44
score: 154.0
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 4..313
NoneNo IPR availablePANTHERPTHR46641:SF12CNMAMIDE RECEPTORcoord: 4..313
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 8..311
e-value: 3.4031E-34
score: 124.668
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 12..318
NoneNo IPR availableTMHMMTMhelixcoord: 186..208
NoneNo IPR availableTMHMMTMhelixcoord: 44..66
NoneNo IPR availableTMHMMTMhelixcoord: 243..265
NoneNo IPR availableTMHMMTMhelixcoord: 12..34
NoneNo IPR availableTMHMMTMhelixcoord: 87..109
NoneNo IPR availableTMHMMTMhelixcoord: 280..302
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 24..300
score: 24.624