SMED30002274

Overview
NameSMED30002274
Smed IDSMED30002274
Length (bp)2168
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30002274 (SMED30002274) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30002274 (SMED30002274) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30002274 (SMED30002274) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30002274 (SMED30002274) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002274

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30002274SMESG000057505.1 SMESG000057504.1 SmedASXL_016590SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30002274SMESG000057505.1 SMESG000057504.1 dd_Smed_v4_8804_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30002274SMESG000057505.1 SMESG000057504.1 dd_Smed_v4_8804_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30002274SMESG000057505.1 SMESG000057504.1 dd_Smed_v4_8804_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30002274SMESG000057505.1 SMESG000057504.1 dd_Smed_v4_8804_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30002274SMESG000057505.1 SMESG000057504.1 dd_Smed_v4_8804_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30002274SMESG000057505.1 SMESG000057504.1 dd_Smed_v4_8804_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30002274SMESG000057505.1 SMESG000057504.1 dd_Smed_v4_8804_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002274SMESG000057504.1 Contig36868newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002274SMESG000057504.1 Contig36868uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
body wall musculatureSMED30002274SMESG000057505.1 SMESG000057504.1 dd_Smed_v4_8804_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30002274 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000043:129370..158846 -9365
Homology
[Close]
BLAST of SMED30002274 vs. Planmine SMEST
Match: SMESG000057505.1 (SMESG000057505.1)

HSP 1 Score: 696.812 bits (1797), Expect = 0.000e+0
Identity = 366/374 (97.86%), Postives = 368/374 (98.40%), Query Frame = 1 
Query:  754 IGKQSKRIPTIEPSTTHQLNSTFESPVVTDHTNLNSPNWIIQNLTRPEXXXXXXXXXXXXXXXXXXXXIRCVLLKSVIDNYNLGIRNGSCLMGCLCIEQYSHRRKYSQSINYSNQVGSDLYRCQALSPDNLALYDDLSAVSNSSARVKLRSNPCSDLDEETMLTNLMPNGGSQINSINVGSVSTSLPRRYTSPSHAAYFHPGLGNYVIVSGPIPSHDQYTIGAAPPGYALQMVSPIPSAPGSITGGHGTNISLQSGNSDDLLDTDHSQTTNSIANQSIKQNNPSVDMIDANRMHDLSQTINFRVNGFPTNRQILINNPINYNRSNGSVPRHPWSMEKVPFPPSSSMTSLPQYSASENGIYVQNKNQIKDPLITS 1875
            +GK S     IEPSTTHQLNSTFESPVVTDHTNLNSPNWIIQNLTRPELVLALVVLLGFSLLLNLSFLIRCVLLKSVIDNYNLGIRNGSCLMGCLCIEQYSHRRKYSQSINYSNQVGSDLYRCQALSPDNLALYDDLSAVSNSSARVKLRSNPCSDLDEETMLTNLMPNGGSQINSINVGSVSTSLPRRYTSPSHAAYFHPGLGNYVIVSGPIPSHDQYTIGAAPPGYALQMVSPIPSAPGSITGGHGTNISLQSGNSDDLLDTDHSQTTNSIANQSIKQNNPSVDMIDANRMHDLSQTINFRVNGFPTNRQILINNPINYNRSNGSVPRHPWSMEKVPFPPSSSMTSLPQYSASENGIYVQ+KNQIKDPLITS
Sbjct:   18 VGKISMFKFAIEPSTTHQLNSTFESPVVTDHTNLNSPNWIIQNLTRPELVLALVVLLGFSLLLNLSFLIRCVLLKSVIDNYNLGIRNGSCLMGCLCIEQYSHRRKYSQSINYSNQVGSDLYRCQALSPDNLALYDDLSAVSNSSARVKLRSNPCSDLDEETMLTNLMPNGGSQINSINVGSVSTSLPRRYTSPSHAAYFHPGLGNYVIVSGPIPSHDQYTIGAAPPGYALQMVSPIPSAPGSITGGHGTNISLQSGNSDDLLDTDHSQTTNSIANQSIKQNNPSVDMIDANRMHDLSQTINFRVNGFPTNRQILINNPINYNRSNGSVPRHPWSMEKVPFPPSSSMTSLPQYSASENGIYVQDKNQIKDPLITS 391
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BLAST of SMED30002274 vs. Planmine SMEST
Match: SMESG000057505.1 (SMESG000057505.1)

HSP 1 Score: 692.96 bits (1787), Expect = 0.000e+0
Identity = 363/364 (99.73%), Postives = 364/364 (100.00%), Query Frame = 1 
Query:  784 IEPSTTHQLNSTFESPVVTDHTNLNSPNWIIQNLTRPEXXXXXXXXXXXXXXXXXXXXIRCVLLKSVIDNYNLGIRNGSCLMGCLCIEQYSHRRKYSQSINYSNQVGSDLYRCQALSPDNLALYDDLSAVSNSSARVKLRSNPCSDLDEETMLTNLMPNGGSQINSINVGSVSTSLPRRYTSPSHAAYFHPGLGNYVIVSGPIPSHDQYTIGAAPPGYALQMVSPIPSAPGSITGGHGTNISLQSGNSDDLLDTDHSQTTNSIANQSIKQNNPSVDMIDANRMHDLSQTINFRVNGFPTNRQILINNPINYNRSNGSVPRHPWSMEKVPFPPSSSMTSLPQYSASENGIYVQNKNQIKDPLITS 1875
            IEPSTTHQLNSTFESPVVTDHTNLNSPNWIIQNLTRPELVLALVVLLGFSLLLNLSFLIRCVLLKSVIDNYNLGIRNGSCLMGCLCIEQYSHRRKYSQSINYSNQVGSDLYRCQALSPDNLALYDDLSAVSNSSARVKLRSNPCSDLDEETMLTNLMPNGGSQINSINVGSVSTSLPRRYTSPSHAAYFHPGLGNYVIVSGPIPSHDQYTIGAAPPGYALQMVSPIPSAPGSITGGHGTNISLQSGNSDDLLDTDHSQTTNSIANQSIKQNNPSVDMIDANRMHDLSQTINFRVNGFPTNRQILINNPINYNRSNGSVPRHPWSMEKVPFPPSSSMTSLPQYSASENGIYVQ+KNQIKDPLITS
Sbjct:   31 IEPSTTHQLNSTFESPVVTDHTNLNSPNWIIQNLTRPELVLALVVLLGFSLLLNLSFLIRCVLLKSVIDNYNLGIRNGSCLMGCLCIEQYSHRRKYSQSINYSNQVGSDLYRCQALSPDNLALYDDLSAVSNSSARVKLRSNPCSDLDEETMLTNLMPNGGSQINSINVGSVSTSLPRRYTSPSHAAYFHPGLGNYVIVSGPIPSHDQYTIGAAPPGYALQMVSPIPSAPGSITGGHGTNISLQSGNSDDLLDTDHSQTTNSIANQSIKQNNPSVDMIDANRMHDLSQTINFRVNGFPTNRQILINNPINYNRSNGSVPRHPWSMEKVPFPPSSSMTSLPQYSASENGIYVQDKNQIKDPLITS 394
[Close]
BLAST of SMED30002274 vs. Planmine SMEST
Match: SMESG000057504.1 (SMESG000057504.1)

HSP 1 Score: 290.041 bits (741), Expect = 3.974e-92
Identity = 162/163 (99.39%), Postives = 162/163 (99.39%), Query Frame = 1 
Query:  292 MFVAEIAIVLVCGIIGRTSAEDKLLINATYSSAHIKLTCSTKIGTGERFQIHLKCPFTDSEICFTDCSGNLTTDGNSLKQMITKCDIISTYDTYSVTYQINIAQVTVTGDWYCIYRGNKSIRIPISKYEIPXXXXXXXXXXXXXXXXXXXXEIFIGKQSKRIP 780
            MFVAEIAIVLVCGIIGRTSAEDKLLINATYSSAHIKLTCSTKIGTGERFQIHLKCPFTDSEICFTDCSGNLTTDGNSLKQMITKCDIISTYDTYSVTYQINIAQVTVTGDWYCIYRGNKSIRIPISKYEIPTVETKTTTTVA KKYAVKTKEIFIGKQSKRIP
Sbjct:    1 MFVAEIAIVLVCGIIGRTSAEDKLLINATYSSAHIKLTCSTKIGTGERFQIHLKCPFTDSEICFTDCSGNLTTDGNSLKQMITKCDIISTYDTYSVTYQINIAQVTVTGDWYCIYRGNKSIRIPISKYEIPTVETKTTTTVATKKYAVKTKEIFIGKQSKRIP 163

HSP 2 Score: 223.016 bits (567), Expect = 1.223e-66
Identity = 130/148 (87.84%), Postives = 138/148 (93.24%), Query Frame = 1 
Query:  340 RTSAEDKLLINATYSSAHIKLTCSTKIGTGERFQIHLKCPFTDSEICFTDCSGNLTTDGNSLKQMITKCDIISTYDTYSVTYQINIAQVTVTGDWYCIYRGNKSIRIPISKYEIPXXXXXXXXXXXXXXXXXXXXEIFIGKQSKRIPT 783
            RTSAE+KLLI +TYSSAHIKLTCSTKI TGERFQIHLKCPFTDSE+CFTDC     TDGNSLKQ+ITKC+  STYDT+S+TYQINIAQVTVTGDWYCIYRGNKSIRIPISKYEIPTVETKTTTTVA KKYAVKTKEIFIGKQSKRIP+
Sbjct:  165 RTSAENKLLITSTYSSAHIKLTCSTKIETGERFQIHLKCPFTDSEMCFTDCDA---TDGNSLKQIITKCEKSSTYDTFSITYQINIAQVTVTGDWYCIYRGNKSIRIPISKYEIPTVETKTTTTVATKKYAVKTKEIFIGKQSKRIPS 309
[Close]
BLAST of SMED30002274 vs. Planmine SMEST
Match: SMESG000074733.1 (SMESG000074733.1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.315e-16
Identity = 110/372 (29.57%), Postives = 163/372 (43.82%), Query Frame = 1 
Query:  307 IAIVLVCGIIGRTSAED---KLL-----INATYSSAHIKLTCSTKIGTGER-FQIHLKCPFTDSEICFTDCSGNLTTDGNSLKQMITKCDIISTYDTYSVTYQINIAQVTVTGDWYCIYRGNKSIRIPISKYEIPXXXXXXXXXXXXXXXXXXXXEIFIGKQSKRIPTIEPSTTHQLNSTFESPVVTDHTNLNSPNWIIQNLTRPEXXXXXXXXXXXXXXXXXXXXIRCVLLKSVIDNYNLGIR-NGSCLMGCLCIEQYSHRRKYSQSINYSNQVGSDLYRCQALSPDNLALYDDLSAVSNSSARVKLRSNPCSDLDEETMLTNLMPNGGSQINSINVGSVSTSLPRRYTSPSHAAYFHPGLGNYVIVSGPI 1392
            I  + + G +  T A D   KLL        TYS   I L C  K    +  F   LKCPFTD+ +C+ DC   L    N L  MI+ C    T  T  +T+ I+I +V  TGDW C Y  + S    + +Y + T +T  +  +             I  +   IP +  +TTH +  T E           +PN     +T P +VL LV ++  SLL N+ FLIRC LL+S+ID +    + + SCL  C CI   +   K  Q+       G+       +      +YDD+SA S+ + R  + +    +L+++++L      G     SI        + R+   P       P   NYVIVSGP+
Sbjct:    4 IQCIFLYGFLVNTLASDPTNKLLNVSPRFTVTYSYVKIVLECDVKYDIKDNNFSTVLKCPFTDNHLCYLDCDA-LCYAENCLP-MISSCSKNQTNTTIKLTWIIDITKVNKTGDWSCSYGSHGSKPAKVERYVMTTGQTDKSLEMITSTTKNV-----IPNKIVNIPNVISTTTHAMQQTQE-------IYQENPNEFWLFITNPNVVLVLVCVITVSLLFNIFFLIRCALLRSLIDQFVQSHKISESCLYRCFCIWINNGLPKSHQNFTSKPNPGNSF-----VGYHREDVYDDMSA-SSQADRHLINNYSQYELEQDSLLARKYWAGSDGSQSI--------MARQ---PQFIYATQPK-SNYVIVSGPV 343
[Close]
BLAST of SMED30002274 vs. Planmine SMEST
Match: SMESG000017122.1 (SMESG000017122.1)

HSP 1 Score: 58.5362 bits (140), Expect = 1.076e-8
Identity = 30/83 (36.14%), Postives = 45/83 (54.22%), Query Frame = 1 
Query:  439 QIHLKCPFTDSEICFTDCSGNLTTDGNSLKQMITKCDIISTYD-TYSVTYQINIAQVTVTGDWYCIYRGNKSIRIPISKYEIP 684
            +IHL CPF D   C+ +C           +++ T C+ +S  + T ++ YQ+++  V VTG W C Y   KS+ I ISKY  P
Sbjct:   62 KIHLFCPFLDGSSCYINCDNQCLYGVGPCRKVFTSCNYVSNNESTINLYYQLDMRFVNVTGQWICQYDEQKSLPIEISKYVQP 144
The following BLAST results are available for this feature:
BLAST of SMED30002274 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30002274 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000057505.10.000e+097.86SMESG000057505.1[more]
SMESG000057505.10.000e+099.73SMESG000057505.1[more]
SMESG000057504.13.974e-9299.39SMESG000057504.1[more]
SMESG000074733.12.315e-1629.57SMESG000074733.1[more]
SMESG000017122.11.076e-836.14SMESG000017122.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002274 ID=SMED30002274|Name=SMED30002274|organism=Schmidtea mediterranea sexual|type=transcript|length=2168bp
GATTTTTCCAATAGAACAACTGTAAATTTTGCTTTTACAATTTTATGATA
TTTCTCCCACTATTAGCATTAAAATAATCAAATATTTTCGAATGTTTTGA
TTAAATTCTTTCTCCAGTCGAATGAAATACATTTCGTTTCATTATTAGTC
CCGGGAAATGCTGAAACGAGTTTCACGAAACCGAAAAGACTTTACCCGAG
TTTTCGTGTCGTTTTTTTGTTCGACTGAATAAAAACATTTTATCTAGTTT
TTATTTACTATGAGACTCGAATAATCTGAGGAATTTTCTAAATGTTTGTA
GCTGAGATAGCCATCGTTCTTGTGTGTGGCATCATCGGGAGAACTTCAGC
AGAGGATAAATTACTCATAAACGCGACTTATTCATCAGCACATATCAAAC
TTACTTGTTCAACTAAAATAGGGACCGGGGAACGCTTCCAGATTCATCTC
AAGTGTCCTTTCACGGATTCCGAGATTTGTTTCACAGACTGCTCTGGGAA
TCTCACTACAGATGGAAACTCATTAAAACAAATGATTACCAAATGTGACA
TAATCTCAACCTATGATACATATTCAGTAACCTACCAAATCAACATAGCC
CAGGTTACTGTGACCGGAGATTGGTATTGTATCTACAGAGGGAATAAATC
TATAAGAATTCCAATCTCGAAATATGAAATCCCAACAGTGGAAACTAAAA
CGACAACCACAGTTGCAAGAAAGAAATATGCTGTGAAAACCAAAGAAATA
TTTATAGGGAAACAAAGCAAAAGAATCCCAACCATTGAACCTTCAACAAC
TCATCAACTGAACTCCACTTTTGAATCACCGGTTGTTACAGATCATACAA
ATCTGAACTCACCTAACTGGATCATTCAAAATTTGACTCGGCCAGAATTG
GTTCTAGCTCTAGTAGTATTGCTTGGGTTTTCTTTGCTACTTAATCTCTC
ATTTTTGATCCGTTGTGTTCTCTTAAAATCAGTTATCGACAATTACAATC
TAGGAATTCGTAACGGATCCTGTTTGATGGGCTGCTTGTGCATCGAACAA
TATTCCCACAGAAGAAAATACTCCCAAAGCATTAATTATTCCAATCAAGT
CGGAAGTGATCTTTATCGCTGTCAAGCATTATCGCCAGATAATTTGGCTC
TTTATGATGACTTGAGTGCAGTCTCAAACAGTTCAGCACGTGTTAAACTA
AGATCGAACCCTTGTTCAGATTTAGATGAGGAAACCATGCTAACCAATTT
AATGCCAAACGGAGGTTCTCAAATTAATTCAATAAATGTAGGATCAGTTT
CAACGTCACTTCCAAGGAGATATACAAGTCCGAGTCATGCAGCCTATTTT
CATCCAGGTCTGGGAAACTATGTTATCGTAAGTGGTCCGATACCTTCTCA
TGACCAGTACACAATTGGAGCTGCACCCCCAGGTTATGCTTTACAAATGG
TCTCACCAATTCCAAGTGCACCTGGCTCGATTACTGGAGGACATGGCACT
AATATATCACTTCAATCAGGCAACAGTGATGACTTGCTTGACACTGATCA
TTCTCAGACTACCAACAGTATTGCAAACCAATCTATCAAACAAAACAACC
CATCAGTTGATATGATTGATGCAAATCGAATGCATGACCTTTCTCAAACC
ATTAATTTCCGTGTCAATGGATTTCCTACAAACCGTCAAATACTGATAAA
TAATCCAATTAATTACAACAGATCAAATGGATCAGTACCTCGACATCCTT
GGTCTATGGAAAAAGTACCTTTTCCGCCTTCTTCTTCAATGACTTCACTA
CCTCAATATTCTGCATCTGAAAATGGTATTTATGTTCAAAACAAAAACCA
AATTAAAGACCCCCTAATAACAAGTTAATTTGAAGTTCTTCATCTCTTAC
ATTGACAAAAAACAAATTCGTTACTTGAAAATGAAAAACTTTTATTATCT
TTTTACAGATTTTATTGTATTTCTTATTTTTACCTTGTTCATTTAGATTT
TTTTATCTAACCAACATTTGCATGAGTGACATCATTTTACAGTCGGTGTT
GATGATTTACTTCGGCAGTTTTCATATTTTTCTTACAAATTAATTATGAT
TTTCACGGTTTTTCAACATCATTTTGTTTATATTGTTGAATTTTTATTTA
ATAAATAATAATGGTCGG
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protein sequence of SMED30002274-orf-1

>SMED30002274-orf-1 ID=SMED30002274-orf-1|Name=SMED30002274-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=529bp
MFVAEIAIVLVCGIIGRTSAEDKLLINATYSSAHIKLTCSTKIGTGERFQ
IHLKCPFTDSEICFTDCSGNLTTDGNSLKQMITKCDIISTYDTYSVTYQI
NIAQVTVTGDWYCIYRGNKSIRIPISKYEIPTVETKTTTTVARKKYAVKT
KEIFIGKQSKRIPTIEPSTTHQLNSTFESPVVTDHTNLNSPNWIIQNLTR
PELVLALVVLLGFSLLLNLSFLIRCVLLKSVIDNYNLGIRNGSCLMGCLC
IEQYSHRRKYSQSINYSNQVGSDLYRCQALSPDNLALYDDLSAVSNSSAR
VKLRSNPCSDLDEETMLTNLMPNGGSQINSINVGSVSTSLPRRYTSPSHA
AYFHPGLGNYVIVSGPIPSHDQYTIGAAPPGYALQMVSPIPSAPGSITGG
HGTNISLQSGNSDDLLDTDHSQTTNSIANQSIKQNNPSVDMIDANRMHDL
SQTINFRVNGFPTNRQILINNPINYNRSNGSVPRHPWSMEKVPFPPSSSM
TSLPQYSASENGIYVQNKNQIKDPLITS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000240body wall musculature
PLANA:0000418head
PLANA:0002032epidermal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..20
score: 0.547