coiled-coil domain-containing protein 81

Overview
Namecoiled-coil domain-containing protein 81
Smed IDSMED30002256
Length (bp)539
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of coiled-coil domain-containing protein 81 (SMED30002256) t-SNE clustered cells

Violin plots show distribution of expression levels for coiled-coil domain-containing protein 81 (SMED30002256) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of coiled-coil domain-containing protein 81 (SMED30002256) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for coiled-coil domain-containing protein 81 (SMED30002256) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30002256

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30002256SMESG000036887.1 SmedASXL_008071SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30002256SMESG000036887.1 dd_Smed_v4_6753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30002256SMESG000036887.1 dd_Smed_v4_6753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30002256SMESG000036887.1 dd_Smed_v4_6753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30002256SMESG000036887.1 dd_Smed_v4_6753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30002256SMESG000036887.1 dd_Smed_v4_6753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30002256SMESG000036887.1 dd_Smed_v4_6753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30002256SMESG000036887.1 dd_Smed_v4_6753_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002256SMESG000036887.1 Contig49386newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30002256SMESG000036887.1 Contig49386uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30002256SMESG000036887.1 dd_Smed_v6_6753_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30002256SMESG000036887.1 dd_Smed_v4_6753_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Human
Match: CCDC81 (coiled-coil domain containing 81 [Source:HGNC Symbol;Acc:HGNC:26281])

HSP 1 Score: 110.153 bits (274), Expect = 8.714e-28
Identity = 53/131 (40.46%), Postives = 79/131 (60.31%), Query Frame = 2 
Query:  110 SLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            SL+  ++  IW  VS+FV   +   KG+ IP  GTF+F + KL++G NK++L+QRP+FI+ EK+ Q H LKQNK    G IP++ LN   + L+  F RD VE CV+  +   + +I+ +   E     IG
Sbjct:   22 SLSQEEVSIIWGNVSEFVRRQLTLHKGVQIPAFGTFTFIRQKLEVGNNKFILIQRPVFIMVEKLVQIHGLKQNKVYTPGEIPIVPLNFVMISLEGPFNRDVVEGCVKETLLFLSRSISMKQNVEFTFKGIG 152
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Human
Match: CCDC81 (coiled-coil domain containing 81 [Source:HGNC Symbol;Acc:HGNC:26281])

HSP 1 Score: 80.1073 bits (196), Expect = 2.166e-17
Identity = 36/74 (48.65%), Postives = 52/74 (70.27%), Query Frame = 2 
Query:  110 SLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNK 331
            SL+  ++  IW  VS+FV   +   KG+ IP  GTF+F + KL++G NK++L+QRP+FI+ EK+ Q H LKQNK
Sbjct:   22 SLSQEEVSIIWGNVSEFVRRQLTLHKGVQIPAFGTFTFIRQKLEVGNNKFILIQRPVFIMVEKLVQIHGLKQNK 95
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Xenopus
Match: cbwd1.1 (COBW domain containing 1, gene 1 [Source:Xenbase;Acc:XB-GENE-5878454])

HSP 1 Score: 121.324 bits (303), Expect = 5.363e-32
Identity = 57/130 (43.85%), Postives = 83/130 (63.85%), Query Frame = 2 
Query:  113 LNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            L + ++ +IW  VS+F+   M  QKG+ IPGLGTF+ ++  L++G NK ++LQRP+F+ SEK+ Q H L  NK   TG IPV+ LN  +L   SSF+RD +E CVR  ++A + +IA +   E     IG
Sbjct:   19 LTEDEVCSIWARVSEFIAHQMALQKGVQIPGLGTFTLSRHTLNVGNNKSIVLQRPVFLFSEKVAQLHGLSYNKIFTTGDIPVVPLNYMTLAFGSSFSRDIIEGCVRDTLSAFSRSIATKQNVEFDFKGIG 148
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Xenopus
Match: cbwd1.1 (COBW domain containing 1, gene 1 [Source:Xenbase;Acc:XB-GENE-5878454])

HSP 1 Score: 121.709 bits (304), Expect = 6.339e-32
Identity = 57/130 (43.85%), Postives = 83/130 (63.85%), Query Frame = 2 
Query:  113 LNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            L + ++ +IW  VS+F+   M  QKG+ IPGLGTF+ ++  L++G NK ++LQRP+F+ SEK+ Q H L  NK   TG IPV+ LN  +L   SSF+RD +E CVR  ++A + +IA +   E     IG
Sbjct:   19 LTEDEVCSIWARVSEFIAHQMALQKGVQIPGLGTFTLSRHTLNVGNNKSIVLQRPVFLFSEKVAQLHGLSYNKIFTTGDIPVVPLNYMTLAFGSSFSRDIIEGCVRDTLSAFSRSIATKQNVEFDFKGIG 148
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Xenopus
Match: cbwd1.1 (COBW domain containing 1, gene 1 [Source:Xenbase;Acc:XB-GENE-5878454])

HSP 1 Score: 115.931 bits (289), Expect = 7.385e-30
Identity = 57/130 (43.85%), Postives = 83/130 (63.85%), Query Frame = 2 
Query:  113 LNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            L + ++ +IW  VS+F+   M  QKG+ IPGLGTF+ ++  L++G NK ++LQRP+F+ SEK+ Q H L  NK   TG IPV+ LN  +L   SSF+RD +E CVR  ++A + +IA +   E     IG
Sbjct:   19 LTEDEVCSIWARVSEFIAHQMALQKGVQIPGLGTFTLSRHTLNVGNNKSIVLQRPVFLFSEKVAQLHGLSYNKIFTTGDIPVVPLNYMTLAFGSSFSRDIIEGCVRDTLSAFSRSIATKQNVEFDFKGIG 148
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Mouse
Match: Ccdc81 (coiled-coil domain containing 81 [Source:MGI Symbol;Acc:MGI:1918134])

HSP 1 Score: 118.242 bits (295), Expect = 2.683e-33
Identity = 55/131 (41.98%), Postives = 83/131 (63.36%), Query Frame = 2 
Query:  110 SLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            +L+  ++ TIW  VSDFVE  +   KG+ I GLGTF+F++ +L++G  K+VL+QRP+FI++EK+ Q H LKQNK    G IPV+ LN   + L+  F RD +E CV+  +   + +I+ +   E     IG
Sbjct:   22 TLSQEEVSTIWANVSDFVERQLTMHKGVQISGLGTFTFSRQQLEVGNKKFVLVQRPVFIMAEKLVQTHGLKQNKVFSPGDIPVVPLNFVMISLEGPFNRDTIEGCVKETLLFLSRSISVKQKVEFTFKGIG 152
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Mouse
Match: Ccdc81 (coiled-coil domain containing 81 [Source:MGI Symbol;Acc:MGI:1918134])

HSP 1 Score: 119.013 bits (297), Expect = 5.347e-31
Identity = 55/131 (41.98%), Postives = 83/131 (63.36%), Query Frame = 2 
Query:  110 SLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            +L+  ++ TIW  VSDFVE  +   KG+ I GLGTF+F++ +L++G  K+VL+QRP+FI++EK+ Q H LKQNK    G IPV+ LN   + L+  F RD +E CV+  +   + +I+ +   E     IG
Sbjct:   22 TLSQEEVSTIWANVSDFVERQLTMHKGVQISGLGTFTFSRQQLEVGNKKFVLVQRPVFIMAEKLVQTHGLKQNKVFSPGDIPVVPLNFVMISLEGPFNRDTIEGCVKETLLFLSRSISVKQKVEFTFKGIG 152
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. UniProt/SwissProt
Match: sp|Q5XIN9|CCD81_RAT (Coiled-coil domain-containing protein 81 OS=Rattus norvegicus OX=10116 GN=Ccdc81 PE=1 SV=1)

HSP 1 Score: 121.324 bits (303), Expect = 4.358e-31
Identity = 58/131 (44.27%), Postives = 82/131 (62.60%), Query Frame = 2 
Query:  110 SLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            SL+  ++ TIW  VSDFVE  +   KG+ I GLGTF+F + +L++G  K+VL+QRP+FI+SEK+ Q H LKQNK    G IPV+ LN   + L+  F RD +E CVR  +   + +I+ +   E     IG
Sbjct:   22 SLSQEEVSTIWGNVSDFVERQLTMHKGVQISGLGTFTFTRQQLEMGNKKFVLVQRPVFIMSEKLVQTHGLKQNKVFSPGDIPVVPLNFVMISLEGPFNRDTIEGCVRETLLFLSRSISIKQNVEFTFKGIG 152
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. UniProt/SwissProt
Match: sp|Q9D5W4|CCD81_MOUSE (Coiled-coil domain-containing protein 81 OS=Mus musculus OX=10090 GN=Ccdc81 PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.740e-30
Identity = 55/131 (41.98%), Postives = 83/131 (63.36%), Query Frame = 2 
Query:  110 SLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            +L+  ++ TIW  VSDFVE  +   KG+ I GLGTF+F++ +L++G  K+VL+QRP+FI++EK+ Q H LKQNK    G IPV+ LN   + L+  F RD +E CV+  +   + +I+ +   E     IG
Sbjct:   22 TLSQEEVSTIWANVSDFVERQLTMHKGVQISGLGTFTFSRQQLEVGNKKFVLVQRPVFIMAEKLVQTHGLKQNKVFSPGDIPVVPLNFVMISLEGPFNRDTIEGCVKETLLFLSRSISVKQKVEFTFKGIG 152
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. UniProt/SwissProt
Match: sp|Q6ZN84|CCD81_HUMAN (Coiled-coil domain-containing protein 81 OS=Homo sapiens OX=9606 GN=CCDC81 PE=1 SV=2)

HSP 1 Score: 110.153 bits (274), Expect = 4.185e-27
Identity = 53/131 (40.46%), Postives = 79/131 (60.31%), Query Frame = 2 
Query:  110 SLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            SL+  ++  IW  VS+FV   +   KG+ IP  GTF+F + KL++G NK++L+QRP+FI+ EK+ Q H LKQNK    G IP++ LN   + L+  F RD VE CV+  +   + +I+ +   E     IG
Sbjct:   22 SLSQEEVSIIWGNVSEFVRRQLTLHKGVQIPAFGTFTFIRQKLEVGNNKFILIQRPVFIMVEKLVQIHGLKQNKVYTPGEIPIVPLNFVMISLEGPFNRDVVEGCVKETLLFLSRSISMKQNVEFTFKGIG 152
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. TrEMBL
Match: C3XUW7 (Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_125375 PE=4 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 5.147e-38
Identity = 66/141 (46.81%), Postives = 89/141 (63.12%), Query Frame = 2 
Query:   95 KRNIFS----LNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIGR 505
            KRN FS    L D D+ ++WD VS FVE  M  QKG+HI GLGTF++   KLD+G NKY+L+QRP+F+L+EK  Q H L+  K   TG IPV+ LN  +L L++ F RD VE CVR ++ A +  +  +   E     IG+
Sbjct:   13 KRNKFSTIPTLTDEDVVSVWDNVSSFVEKQMSMQKGVHIAGLGTFTYVHKKLDVGNNKYILIQRPVFLLAEKFAQTHQLEATKHYTTGEIPVVQLNFAALSLETPFDRDTVEACVREVLGALSRAVGSRRNVEFTFNGIGK 153
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. TrEMBL
Match: A0A1S3HGJ9 (coiled-coil domain-containing protein 81 OS=Lingula unguis OX=7574 GN=LOC106155082 PE=4 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 5.760e-38
Identity = 68/142 (47.89%), Postives = 92/142 (64.79%), Query Frame = 2 
Query:   95 KRNIFS-----LNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIGR 505
            K+N FS     LND D+  IWD +S F+E  ML QK +H+PGLGTF+F Q KLDIG NK++L+QRP+F+LSEK  Q H L+  K  V+G IPV+ LN  ++  +S F RD VE CV+ ++ A   ++A +   E     IGR
Sbjct:   14 KKNKFSIIGAKLNDGDVVAIWDNISAFIEKQMLNQKAVHLPGLGTFTFTQKKLDIGNNKFILIQRPVFVLSEKFAQTHGLQYTKHHVSGQIPVVQLNFSAISFESPFDRDAVEICVQEVLGALARSVANKRNVEFTFAGIGR 155
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. TrEMBL
Match: H2ZFM5 (Uncharacterized protein OS=Ciona savignyi OX=51511 PE=4 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 5.409e-37
Identity = 64/140 (45.71%), Postives = 93/140 (66.43%), Query Frame = 2 
Query:   95 KRNIFS----LNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            ++N FS    L++ DI ++WD VS++VE  M  Q+G+HIP LGTF+F   K+DIG NK++L+QRP+F+LSEK  Q H L+  K   TG IPV+ LN  SL ++S F RD VETCVR ++ + + +++     E +   IG
Sbjct:   13 RKNRFSAIPNLSEEDIISVWDAVSEYVELQMCTQRGVHIPNLGTFTFTHKKMDIGNNKFILIQRPVFLLSEKFAQTHALQYTKHHTTGEIPVVQLNFTSLAVESPFDRDTVETCVREVLQSLSRSVSNLQNVEFLFRGIG 152
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. TrEMBL
Match: K1Q9X3 (Coiled-coil domain-containing protein 81 OS=Crassostrea gigas OX=29159 GN=CGI_10013577 PE=4 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 1.537e-35
Identity = 68/153 (44.44%), Postives = 99/153 (64.71%), Query Frame = 2 
Query:   47 ELENNNSDIITDRKLFKRNIFSLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIGR 505
            E ++N   +I D+         L++ D+ ++W+ VS+F+E  M +QKG+ IP LGTF+F Q KLDIG NK++++QRP+F+LSEK  Q H L+  K  V G+IPVI LN  +L  +S F RD VE CVR ++ A +  ++G+   EL  T IGR
Sbjct:   11 EAKSNKYSLIKDK---------LSEDDVASVWENVSNFIEKHMSQQKGVSIPMLGTFTFTQKKLDIGNNKFIMMQRPIFMLSEKFAQTHGLQSVKYHVPGNIPVIQLNYAALSFESPFDRDTVENCVREILGAVSRGVSGKKNVELTFTGIGR 154
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. TrEMBL
Match: A0A1I8I657 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 2.197e-35
Identity = 66/125 (52.80%), Postives = 85/125 (68.00%), Query Frame = 2 
Query:  137 IWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSS--FTRDQVETCVRXLITATNNTIAGQNGTELVXTRIGR 505
            IW+ VS F+E  +  QKG+HIP  GTFSF Q KLD+G  KY+L+QRP+F L E+I Q H L Q K  VTGS+PV+ LN  +L ++SS  F RD++ETCVR  + A N +IA Q   ELV   +GR
Sbjct:   35 IWEAVSRFIEKNLQNQKGVHIPNFGTFSFVQKKLDVGNAKYLLIQRPVFQLFERICQTHGLAQTKYHVTGSVPVVQLNYAALSVESSGHFDRDKIETCVRETLGALNRSIAMQRNVELVFCGVGR 159
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Cavefish
Match: CCDC81 (coiled-coil domain containing 81 [Source:NCBI gene;Acc:103040579])

HSP 1 Score: 108.997 bits (271), Expect = 1.184e-27
Identity = 54/105 (51.43%), Postives = 72/105 (68.57%), Query Frame = 2 
Query:  113 LNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCV 427
            LN  D+  IW  V+ F+E  M  QK + I GLGTF+F+Q+KLD+G +KYVL+QRP+F LSE++ Q H LK  KA   G IPV+ LN  +L ++S F RD VE C+
Sbjct:   23 LNKHDVDHIWANVASFIERQMALQKAVCISGLGTFTFSQLKLDVG-SKYVLIQRPIFQLSERLCQTHGLKHTKALAAGDIPVVPLNFTALSVESPFDRDVVEGCI 126
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001530.1 (pep scaffold:Pmarinus_7.0:GL476341:577018:598505:-1 gene:ENSPMAG00000001379.1 transcript:ENSPMAT00000001530.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 77.411 bits (189), Expect = 2.586e-17
Identity = 39/106 (36.79%), Postives = 58/106 (54.72%), Query Frame = 2 
Query:  185 KGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIG 502
            +G+HIP LGTF+F   +L +   + V ++RP+  +SEK+ Q H L  +K    G IPV+ LN  +L L+S  +R Q E  V  L+      +AG    EL    +G
Sbjct:    1 QGVHIPELGTFTFALHRLPMSGGRSVAVRRPVLRVSEKLIQTHGLTHSKPHPPGEIPVVQLNWTALALESGVSRAQAEGAVHCLLRTLERALAGGRSVELGLGAVG 106
[Close]
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Nematostella
Match: EDO38720 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC01])

HSP 1 Score: 128.642 bits (322), Expect = 5.370e-35
Identity = 64/143 (44.76%), Postives = 89/143 (62.24%), Query Frame = 2 
Query:   95 KRNIFS----LNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIGRXA 511
            K+N FS    L + DI ++WD +SD++   M  QK   +PGLG F+F+Q KLD+G NKY+L+QRP+F+LSEK    H L   K   TG IPV+ LN  +L   +SF RD VE+CV+ ++ A N +I  +   E   T IGR +
Sbjct:   13 KKNRFSAIPKLTEEDIVSVWDNLSDYIARQMGSQKASFVPGLGIFTFSQKKLDVGNNKYILIQRPVFVLSEKFAMTHGLSYTKHYTTGQIPVVQLNFVALSNNTSFDRDTVESCVKEVLQALNRSIQSKRNVEFQFTGIGRLS 155
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BLAST of coiled-coil domain-containing protein 81 vs. Planmine SMEST
Match: SMESG000036887.1 (SMESG000036887.1)

HSP 1 Score: 313.153 bits (801), Expect = 9.081e-104
Identity = 151/156 (96.79%), Postives = 151/156 (96.79%), Query Frame = 2 
Query:   38 MSNELENNNSDIITDRKLFKRNIFSLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKIXQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATNNTIAGQNGTELVXTRIGR 505
            MSNELENNNSDIITDRKLFKRNIFSLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKI QNHNLKQNKAQVTGSIPVITLN  SLMLKSSFTRDQVETCVR LITATNNTIAGQNGTELV TRIGR
Sbjct:    1 MSNELENNNSDIITDRKLFKRNIFSLNDSDIQTIWDFVSDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKICQNHNLKQNKAQVTGSIPVITLNYSSLMLKSSFTRDQVETCVRELITATNNTIAGQNGTELVFTRIGR 156
The following BLAST results are available for this feature:
BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 2
Match NameE-valueIdentityDescription
CCDC818.714e-2840.46coiled-coil domain containing 81 [Source:HGNC Symb... [more]
CCDC812.166e-1748.65coiled-coil domain containing 81 [Source:HGNC Symb... [more]
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BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
cbwd1.15.363e-3243.85COBW domain containing 1, gene 1 [Source:Xenbase;A... [more]
cbwd1.16.339e-3243.85COBW domain containing 1, gene 1 [Source:Xenbase;A... [more]
cbwd1.17.385e-3043.85COBW domain containing 1, gene 1 [Source:Xenbase;A... [more]
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BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Ccdc812.683e-3341.98coiled-coil domain containing 81 [Source:MGI Symbo... [more]
Ccdc815.347e-3141.98coiled-coil domain containing 81 [Source:MGI Symbo... [more]
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BLAST of coiled-coil domain-containing protein 81 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q5XIN9|CCD81_RAT4.358e-3144.27Coiled-coil domain-containing protein 81 OS=Rattus... [more]
sp|Q9D5W4|CCD81_MOUSE3.740e-3041.98Coiled-coil domain-containing protein 81 OS=Mus mu... [more]
sp|Q6ZN84|CCD81_HUMAN4.185e-2740.46Coiled-coil domain-containing protein 81 OS=Homo s... [more]
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BLAST of coiled-coil domain-containing protein 81 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
C3XUW75.147e-3846.81Uncharacterized protein OS=Branchiostoma floridae ... [more]
A0A1S3HGJ95.760e-3847.89coiled-coil domain-containing protein 81 OS=Lingul... [more]
H2ZFM55.409e-3745.71Uncharacterized protein OS=Ciona savignyi OX=51511... [more]
K1Q9X31.537e-3544.44Coiled-coil domain-containing protein 81 OS=Crasso... [more]
A0A1I8I6572.197e-3552.80Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CCDC811.184e-2751.43coiled-coil domain containing 81 [Source:NCBI gene... [more]
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BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000001530.12.586e-1736.79pep scaffold:Pmarinus_7.0:GL476341:577018:598505:-... [more]
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BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO387205.370e-3544.76Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of coiled-coil domain-containing protein 81 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of coiled-coil domain-containing protein 81 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000036887.19.081e-10496.79SMESG000036887.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30002256 ID=SMED30002256|Name=coiled-coil domain-containing protein 81|organism=Schmidtea mediterranea sexual|type=transcript|length=539bp
GAGGGCAACATCGCAGTTCTCTGTGCAAGTCAAAAAAATGTCAAACGAGC
TGGAAAATAATAATAGTGACATTATTACTGATCGTAAACTATTCAAAAGA
AACATATTTTCATTAAATGATTCTGATATTCAAACCATTTGGGATTTTGT
ATCAGATTTTGTGGAATCGACTATGCTTAAGCAAAAAGGTATTCATATTC
CAGGTTTGGGAACCTTTTCATTTAATCAAGTAAAACTGGACATTGGACAG
AACAAATATGTTCTACTTCAGAGACCTCTATTTATATTATCGGAAAAAAT
TTGNCAAAACCACAATCTAAAACAAAACAAAGCTCAAGTCACAGGCTCGA
TTCCGGTGATAACTCTCAATTNTNCCTCCTTAATGCTGAAGTCATCATTC
ACAAGGGACCAAGTGGAAACTTGCGTCAGANAATTAATAACTGCTACTAA
CAATACCATTGCCGGTCAGAACGGCACAGAATTGGTGTNTACAAGAATTG
GNCGTTTNGCAAATTCGAAATCNACTANTANAATTCAAA
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protein sequence of SMED30002256-orf-1

>SMED30002256-orf-1 ID=SMED30002256-orf-1|Name=SMED30002256-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=179bp
RATSQFSVQVKKMSNELENNNSDIITDRKLFKRNIFSLNDSDIQTIWDFV
SDFVESTMLKQKGIHIPGLGTFSFNQVKLDIGQNKYVLLQRPLFILSEKI
XQNHNLKQNKAQVTGSIPVITLNXXSLMLKSSFTRDQVETCVRXLITATN
NTIAGQNGTELVXTRIXRXANSKXTXXIQ
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR040673CCDC81_HU_dom_2
IPR026295Coiled-coil_dom_cont_p_81
IPR028034HU-CCDC81
Vocabulary: molecular function
TermDefinition
GO:0003677DNA binding
Vocabulary: cellular component
TermDefinition
GO:0005737cytoplasm
GO:0005813centrosome
GO:0005815microtubule organizing center
GO:0005856cytoskeleton
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040673CCDC81, HU domain 2PFAMPF18289HU-CCDC81_euk_2coord: 117..177
e-value: 1.4E-11
score: 44.3
IPR028034CCDC81, HU domain 1PFAMPF14908HU-CCDC81_euk_1coord: 37..107
e-value: 2.1E-19
score: 69.4
IPR026295Coiled-coil domain-containing protein 81PANTHERPTHR14362FAMILY NOT NAMEDcoord: 32..177